LOCUS       DAOOQW010000001        55643 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_643_length_55643_cov_6.053267, whole genome shotgun sequence.
ACCESSION   DAOOQW010000001 DAOOQW010000000
VERSION     DAOOQW010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 55643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 55643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55643
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_643_length_55643_cov_6.053267"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(316..537)
                     /locus_tag="PK281_00005"
     CDS             complement(316..537)
                     /locus_tag="PK281_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007097026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY47744.1"
                     /translation="MIQSISDFFERQAFGVCTWLGDKLRMKSSQIRLFFIYASFLTLG
                     SPIIVYLHMAFILRIKNLIQSSKTRVWDL"
     gene            653..2284
                     /gene="pruA"
                     /locus_tag="PK281_00010"
     CDS             653..2284
                     /gene="pruA"
                     /locus_tag="PK281_00010"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HOY47745.1"
                     /translation="MIKGFFHLPKAVNEPVKSYAPGSAEKTELKAMLQHLQSVQLDIP
                     MEIGGRSVFTDQKIELRQPHKRKHVLGTFSRGTEQHVKDAIDAALAAKANWEKMSWEN
                     RSAIFLKAADLIAGPYRARLNAATMLGQSKSAYQAEIDSACEIIDFLRFNVQYMSQIY
                     SQQPESSAGIWNRVEQRPLEGFVFALTPFNFTAIAGNLPTSAAMMGNVVVWKPADTQI
                     YAAHVLMEIFKEAGVPAGVINLVFVSGPTAGNVIFSHPDFAGIHFTGSTGVFQNIWKT
                     IGDNIHKYKTYPRIVGETGGKDFIMVHSSAQPAVVATAIARGAFEYQGQKCSAASRCY
                     VPDNIWPAVKEQLVRDMASFKMGPVDDFSNFINAVIDEKSFDKLAAYIDAAKNDPNVE
                     IVAGGTYDKSEGYFIAPTVILAKDPKYTTMCEELFGPVVTIYVYEAAKFSETLKLVDE
                     TSIYALTGSIISQDRYAVDEATYALRNAAGNFYINDKPTGAVVGQQPFGGARGSGTND
                     KAGSMINLLRWVSPRTIKETFVPPTDYRYPFLKES"
     gene            2554..5373
                     /locus_tag="PK281_00015"
     CDS             2554..5373
                     /locus_tag="PK281_00015"
                     /inference="COORDINATES: protein motif:HMM:NF024972.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallo-peptidase"
                     /protein_id="HOY47746.1"
                     /translation="MKNYLLASLAFLCLILSVSAKDGEGSQLFQNVQRNFARTAPQSF
                     SLFKPSNRVYVPGSVKGIENATFLTIDQNPFYQLFTTQPETVTLQVPAPDGSGTWKLD
                     LYRSQILSSDFEVKTSDGKKFSAPEAIYYQGIFNNDPQSMAVVSVFENQLMGMLSNSH
                     GNYDLGVYGEDQLSEYVFYRSNEVGRQLPFDCHTSDVDEPVETALVAGDCRVVRVYLE
                     CDFDLYTKRTSVANVTTFVTGFFNQVAAIYTNENIQVQISQIYVWTTTDPFISQTTSS
                     GVLSAFRTTRTTFNGNIAHLISTRSTNMGGVAYLDVLCATTYRHAFSNIYNSYGSFPT
                     YSWTVNCFSHEMGHNFGSNHTQWCGWTGGALDNCYTTEGGCAAGPAPVNGGTIMSYCH
                     VTGYGVNLANGFGTQPGNKIRTKYLASTCLTGGVAISVVPAGATICSGSNITLTASGA
                     TSYTWTPTTGLNVPNTASVIATPSATSTYTATSVVNNCTVTASSIVTVLASVNRGTLA
                     SGNQTFTTSGDPAAISFSTAATGGSGTFAYQWYSKAGIQTAPTGTSTTGWTAISGATS
                     ATYNPGVQTASISYAVTVDATGTVDCGGAAWASGVRQITVNASTGFSAGTLTSSNQTF
                     CSTGGDPANIIFSVAPSGATSFSYQWYYKNGIAAAPTGTSTTGWTIINGATASTYDPA
                     SGLTQSRTYACRVTPSTGTATWASGARQITILPAFNPGTVASGDQSFCATGNPGAINM
                     SANPVGSGAYTWRWYYKESTSIACPTGNSITGWLTNTTSANISGTTLTGAGVTFDPIS
                     AGAINNGRTFAVLITPIANGSVPACGTAAWASNCRKTYVTTCTGAMPGEELVEGSQLD
                     LGQSFPNPTNSHSTIQYSLPDSYNGARLVFYDQLGRIMSEQVVPTGMANEYEFKGDFL
                     PSGTYYYSLEFAGTKLATKKLVLIH"
     gene            complement(5460..7532)
                     /locus_tag="PK281_00020"
     CDS             complement(5460..7532)
                     /locus_tag="PK281_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007650067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HOY47747.1"
                     /translation="MNKAFILMAALAFTTASQPILAQTTKPGKVRPDSKPEEFKVEAD
                     RFADVQVLRYQIPGFESLNIKQKELAYYLYEAALSGRDMIWDQKYRNNLKVRRTIEAI
                     WNSYKGPKSTPDFLKFEEYSKQVWFSNGIHHHYASDKIIPTFSFESFSAWVKASDSKQ
                     LPLAKDQTVDQFLAFLKPVMFDPTVDAKTVNLASGIDNITGSANNFYESVTQKEVEDY
                     YAAKSNPLDKTPIEYGLNSKLKKVDGKIVEIPWKVGGMYSASIEKIVYWLEKAVPLAE
                     TPEQRKSLELLVEYYKTGDLKKFNEYNIAWVSDVNSTIDNVNGFIEVYQDALGKRGSF
                     EAVVSLKDFEATKKIETIAKNAQWFEDNSPLDPKHKKKEVKGIIGKSIVVIVESGDSS
                     PSTPIGINLPNSDWIRAEHGSKSVALGNIVNAYNVASAKSPVTDEFSLNDETKERARK
                     YGNLASELHTDMHEVIGHASGQLEDGVATPDITLKNYQNALEEARADLVALYYITDPK
                     LVEIGVMPSTDCGKAEYDSYLLNGLMLQLNRVEIGKEIEEAHMRNRQLVAKWVYEKGK
                     ANNVVELIKRDGKTYVQINDYVKLRALFGDLLREIQRIKSQGDFAAGQDLIENYGVKV
                     DPAIHKEVKDRFSKLASAPYKGFIQPRLVPVIEGGKFVDVKVEYPASFSSQMLDYGKR
                     YSFLPNEN"
     gene            complement(7588..7956)
                     /locus_tag="PK281_00025"
     CDS             complement(7588..7956)
                     /locus_tag="PK281_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008239462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HOY47748.1"
                     /translation="MRGYYTLGLLVLSNVFMTLAWYGHLYMKKSSPLMKWGLLGIILL
                     SWGLAFFEYVFQVPANRIGHESNGGPFSLIQLKVIQEVISLTVFALMAVFVFRTEKFD
                     WNHVAAFICLVLAVWFTFRK"
     gene            complement(7956..9605)
                     /locus_tag="PK281_00030"
     CDS             complement(7956..9605)
                     /locus_tag="PK281_00030"
                     /inference="COORDINATES: protein motif:HMM:NF024500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OstA-like protein"
                     /protein_id="HOY47749.1"
                     /translation="MQVETAIDIDAKRSFQSFNILYICAILMNDSFARFFRGEFPVLL
                     MLMLLSTSMLSAQTGRKINIKSANTLEFDDRVDAQRLVGNVIFEHDGTLLYCDSAYLY
                     DKENRLEAYSNVRIVGDSVVVTGQNLTYDGKTKIADIVGNVRMTDPSSVLTTNRLQFD
                     NNTKQARYTTGGKIISEKNELVSQFGMYNSTNRTFYFRKNVKLTNKDYVMVCDTLQYS
                     TVNKTSYFFGPTTITSKNNLIYCENGYYNTKTDISEFGKNARLDSKKQQIRAEKLFYN
                     RKTGDGRARQNVRISDTSQAVVLTGHFADYNEFTGRILMTDSAVMEKEFGKDTLFLHG
                     DTLRSVLDTVTDKRTLFAYHKVKFFKKDFQGRCDSLTYSELDSMMRLYKDPVIWNEEN
                     QLTADTVRIQFANNNLETLYLRTNGFIASQEDSVHFNQISGRNVTGYFVKNELVKIEV
                     VANGESVYYAQGDGDGYMGVNKAICNDMNIYIDSNKVQSITFISDPNATLYPVNEPTK
                     EEIQLRGLRWLGEHRPAKRSDIFIWKNAPAPVKPARRRQKA"
     gene            9656..10708
                     /locus_tag="PK281_00035"
     CDS             9656..10708
                     /locus_tag="PK281_00035"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009415799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HOY47750.1"
                     /translation="MSFPVTRVTESRLPSINLDHLPFGKIFSDHMFIAEYDGKNWINP
                     RIEPYAPISLSPAASVFHYGQAIFEGMKVFRSDKDELLFFRPEENYKRMQQSAIRLCM
                     PPVPEHIFMDGLRQLVDLDAAWVPKGDENSLYVRPVLFATEPVLGVKPSETYSFIILT
                     SPTGAYYTEDLKVVIEKTYSRASRSGTGFAKAAGNYAGALYPTQLAQQKGYNQVIWTD
                     AATNTKVEESGTMNLMFVINGVLITPSLSPSKLAGITRDSVIELARHWGMLVQERDVS
                     VSEIIGAFRAGTLEEAFGTGTAANIAHISVIGHEGEDFKVPELHADLFSVKALRFLND
                     LKAGRTADPFGWITKL"
     gene            complement(10712..11980)
                     /locus_tag="PK281_00040"
     CDS             complement(10712..11980)
                     /locus_tag="PK281_00040"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002673242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HOY47751.1"
                     /translation="MKLDILLGLQWGDEGKGKIVDVLSPEYEVIARFQGGPNAGHSLE
                     FNGIKHVLHTIPSGIFHPECMNIVGNGVVIDPVIFKKEIDALSKLGVDAHKNLHLSRK
                     AHLILPTHRLLDAASEASKGTSKIGSTLKGIGPTYMDKTGRNGLRVGDIMRPDFMERY
                     RFLVQKHEEMLAHYQFNYDLSELEPAFFEGIEHLRGFKHIDSEHILNHQLKAGKKVLA
                     EGAQGSLLDIDFGSYPFVTSSNTISAGACTGLGVAPGRIGEVLGIFKAYCTRVGSGPF
                     PTELENETGEKIRQIGREYGATTGRPRRCGWLDLPALKYSILINGVTQLIMTKADVLS
                     GFETVKVCTSYLVDGVETDEFPFDIDPRYVQPVFTDLPGWNEDLTGIRSLDQFPESLK
                     AYIRYIEQALEVPVTIVSVGPDREQTIRLR"
     gene            complement(11970..12344)
                     /locus_tag="PK281_00045"
     CDS             complement(11970..12344)
                     /locus_tag="PK281_00045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOY47752.1"
                     /translation="MAFSFELDESLAFPVFSLEGSLMERHEAQELLDETAELFAENKA
                     RIILDLQALDYLNSSGLNVLINIFTRARNAGGEVIMCNINDKLLRLLVITKLDAVIQQ
                     TKTTDEAIQVLSVGKSEQKNEA"
     gene            complement(12344..12826)
                     /locus_tag="PK281_00050"
     CDS             complement(12344..12826)
                     /locus_tag="PK281_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HOY47753.1"
                     /translation="MSDQDTKDTVKQIFSDYLEQNGHRKTPERFAILDEIYSHNGHFD
                     IESLYVYMKNKKYRVSRATLYNTIELLLTCNLVTRHQFGKNQAQFEKSYKYKQHDHLI
                     CSDCDKVFEFCDPRIQQIQSMTGELLNFNISSHSLTFYGKCNKLSEKGFCENHPNQKS
                     "
     gene            complement(12896..>14446)
                     /locus_tag="PK281_00055"
     CDS             complement(12896..>14446)
                     /locus_tag="PK281_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HOY47754.1"
                     /translation="FVNPIRNSLTEQGFTFDIKGRPKSIYSIYNKIKIKGIPFEEIFD
                     IFAIRIIIDSPVETEKADCWRVYSIITDIYQPSPDRLRDWISTPRANGYESLHTTVMS
                     PTGKWVEVQIRSRRMDDIAERGYAAHWKYKERNDYESGIDEWLGRIREMLENSDTNAL
                     EFINDFKLNLFSEEIFVFTPKGELKTLPSGSSALDFAFDIHSEVGMACIGAKVNNKLV
                     PLSHKLKSGDQIEILSSSKQKPKEDWLNFVVTAKARSKIKSALKEEKRKVADEGKDIL
                     EKKLKANKIALDGNIINNLLKHFKVNSQLDLFYSIGEGIIKLENIAKIAKGEHNSWYK
                     VLRDKFKRSDSDSKEDQPVTLENIVKDIRGKTDMLVIGEDMDRIDYKLATCCNPIPGD
                     EVFGFVTINEGIKIHKTNCPNAISLMSNYAYRIVKARWMNQQQLAFMAGIRVNGIDDV
                     GIVNNITRIISNELNVNMRSISFDSLDGVFEGNIMVFVHDTNHLTSLMGKIKQVKGVT
                     SVVRIDSN"
     assembly_gap    14447..14546
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<14547..14959)
                     /locus_tag="PK281_00060"
     CDS             complement(<14547..14959)
                     /locus_tag="PK281_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOY47755.1"
                     /translation="MSAPVIDLEKEKKEILKRYTALLRPLADKIDKHEKQQIRKAFDL
                     ALDAHKDMRRKSGEPYIYHPIAVAQIAVSEMGLGATAVICALLHDTVEDTDVTLEDIR
                     SMFGETEAKIIDGLTKIENVFDQSSSIQAENFRKI"
     gene            complement(15053..17164)
                     /gene="ppk1"
                     /locus_tag="PK281_00065"
     CDS             complement(15053..17164)
                     /gene="ppk1"
                     /locus_tag="PK281_00065"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003917.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HOY47756.1"
                     /translation="MSVKRTFNRDLSWLSFNQRVLQEAKDLRVPLYERMKFLAIFSSN
                     LEEFYRIRVAEWKRLMELTKKTKKELREDPREVIKKINKTVKKHLNEFNDIFWNQIVV
                     ELEGRNINLVNEKNLTPEQVVFVQEYFREKVLPHIRPVMINTRRNTPELDEKSIYLAV
                     KLCDFDRPGPRVYDYAITEIPSRKISRFLILPDKNGQHYVMILGDVIRLCINELFPDY
                     ELIDAYSIKLTRDAEMMLEDEFSGSLLKKIKRGLSNRAKGTPTRLLYDSAMPADFLSH
                     LKKIFSIGNQDLLPGGRYHNFSDLMNFPRFDQKGIYYENLPCLKKKTLDESPSMFEAI
                     SKRDFLIHFPYHSYDYVIRFLNEAAEDVKVKAIKITLYRVAPHSKVVEALIKAAQNGK
                     NVTAFVELKARFDEESNISYSKELEEAGVKVLYSFPILKVHAKMVCIERMEDKLRRYV
                     YLSTGNFHENTSRLYCDSSLFTKDKYMGREVSAVFDILADTRIKREFKHLLLAPDHMR
                     HDLYTLIDQEIKNALKKKDAYIYLKLNSLQDAAIIDKLYEASQEGVEIRLIIRGICCL
                     IPGIKGLSENIKVISIVDRFLEHSRIYIFCNENKPKVYLSSADWMERNLSRRFEVGFP
                     IYQEDLRNEVLEIFRLQWYDNTKARIINKIQNNTYRKSVAKERGRAQIDIYQYLSDKS
                     DWKEEPTRNYQERPTTTVISK"
     gene            complement(17166..17660)
                     /locus_tag="PK281_00070"
     CDS             complement(17166..17660)
                     /locus_tag="PK281_00070"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOY47757.1"
                     /translation="MKTLYIVRHAKSSWEQEELKDFDRPLTDRGIKNAPEMAVRILTN
                     GAAPQLLLSSPAKRAISTARLMAIAFKLQEEKIKQDSSIYEAERNDLSRLISRQDPDI
                     DTILLVGHNPGVSDYINWLCGTEEAQIPTCAVARIRVNSNRWNGWEKGMGKLEDLDFP
                     KKKQ"
     gene            complement(17739..19565)
                     /gene="dacB"
                     /locus_tag="PK281_00075"
     CDS             complement(17739..19565)
                     /gene="dacB"
                     /locus_tag="PK281_00075"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HOY47758.1"
                     /translation="MKVFTRITILLICLVYSNRFDIQAQSNSFTEATRKMMQSNATKG
                     AVVSWCFRDVETGQILSTYQEQTYLMPASTAKLFSTAAALKILGADFTFKTQIVIQGE
                     IKDDVLRGDLCVIGGGDPTLGSGKAGAMTAENILEKIYQILHEKGIRKIKGNLIIDPY
                     FFRFDHTAVPRNWPWEDIGNYYGAGSYGLNWRENSFQMSFSPGDTIGAISKYSRPIPE
                     LYNVEFRNAIKAGNTPEEIYVYSAPLSSIIYLDGVQKPKGESQTERGSIPNPPAQFIG
                     ELDSYLKNKGIRISGKSQVAGIQNSSLVNCDTLGVIASPPLLNIVNEINHTSHNLFAE
                     TVGKMLGKQAKIGTRAEDGAQAIRGYLKNSLFLSDSLMILQDASGLSRTNHISTAFQT
                     SFLRSVASQSGFEDFIKTIPEAGKSGSMKSFPEIPNVRAKSGSIGGVRGYAGYLKDVN
                     DRKIAFSVLLNNFNAPSSEIKQLCAELIREAQSGGLPETFKFFPFHLISDTLYSLPEI
                     TWFDKSLKENPTWQDLNTTDREDLRLRMYLSGEPTRENPYKIAVFRSKKDPWNDRFRF
                     RIHAITRKIERWDESSKTFVPSGSDSNEQVEKTKTRKRQNKK"
     gene            19631..20719
                     /gene="aroC"
                     /locus_tag="PK281_00080"
     CDS             19631..20719
                     /gene="aroC"
                     /locus_tag="PK281_00080"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013072129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HOY47759.1"
                     /translation="MAGNSHGVLFRFSSFGESHGLAIGGLIDGCPAGLKLDLLAIQHQ
                     LDRRRPGQSNISTSRKESDTVQFLSGIFEEKTTGAPIGFTIPNSDQHSSDYDALKDVY
                     RPSHADYTYQAKYGIRDHRGGGRSSARETATRVVAGSVARQLLALQHIRIVAFVSQVG
                     EVILPLPTTIPSEKQVDESTVRCPDLEFAKSMEALILQAKEEGDSLGGVITCMIAGVP
                     AGWGEPVFDKLHADLGKAMLSINAVKGFEIGSGFSAAAMRGSVHNDAFIQNEQGEIRT
                     ITNHSGGIQGGISNGELIYFRVAFKPVASIRISQETVSQETGNTKIEIPGRHDPCVVP
                     RAVPIVEAMAALVLADHYLRSKAINSNL"
     gene            20744..23446
                     /locus_tag="PK281_00085"
     CDS             20744..23446
                     /locus_tag="PK281_00085"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HOY47760.1"
                     /translation="MLKKILKITGITFLLLFIMALILPFLFKDKIIAKVKEEANKNLN
                     AKLEFRDLSLGLISSFPNFNIELQGLSLSGINEFKSDTLISAESLAITTDIMSVIGGG
                     EIAIKSIDVDKARIHLKVLKDGKANWDITKPSATPETPSTEPSTFKAKLQEYSLKSSR
                     IWYEDQTMDFTALLDGINHSGSGDFTADVTNLETLTTIDSMDVVYGGIAYLKRANLEY
                     KADFNLDLKNSVYAFKDNELRLNNLLLKFAGSIAMPANDITMDITYEALKNDVKNFIS
                     IFPGAFTKDFEQVKSSGKLAFNGFVKGVYNEKSIPGFAFNLLIENGSIQYPSLPRGIS
                     NLQVKTSITCPGVDVNKTVIDVSKLHADLGQFPIDAHMIVKTPISDPDIDAAIKGKVE
                     LGTLKDMLPLEAGTNLTGEVAADVSLKGHMSAVESGKYEQFAAAGTMSVARFRYAAKD
                     LPQEIQINQAALNFNPKAAQLSQFDMQLGKSDIHATGSLENYLAYVFRDEAITGNLSL
                     TSNFLDLNPFMTTETGTTPPPSNASQPADVNGYIRVPQNIGFTLNSSIKTLLYDNMRL
                     ENVSGQLLVKDQSVRLNQLKMNTLGGAITMGGLYDTQSETGPSVALMLDMSNLNIRET
                     AKTFNTVKQLAPIAENTTGSASIKNFNFSCKADKAFNPDLKSINGSGTLSTSALEIEG
                     FEMVKRIAGALKIDKLKKWKMEPVNAGFTITNGKVTLKPFKTKIGNYATEIGGENGLD
                     QTINYAVHIDIPRAEFGGAANSVLNGLVSKANSNGLQTQVGDMIPVAILVTGTFKDPK
                     VKTDIRNQANNAMNDLKNQAEQRLKDEAERQKKELLDKANAEKERLKNEAENKINQEK
                     EKAKQELDKAKSEAERKAKEEADKAKKKAEEEAKKNLNKLLKGK"
     gene            23450..25408
                     /locus_tag="PK281_00090"
     CDS             23450..25408
                     /locus_tag="PK281_00090"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOY47761.1"
                     /translation="MSFRRFLIVWLLLLPALSLLAQEKEDPCPLSSNKKARKLYEKAI
                     DAARSNKNEARSILTEALELDPEFGRANYMMADLLIKAKKSSEAEPFLKQVAAICPDL
                     DPLTFYRLGSIQFGAKRYQEAKTNLSRFLESNGGKEKDRSDAREQLTAATFYLEGFAK
                     PVPFDPKPLASISSEADEYLPVITADNQRAYFTRRTMEKAKFTGGIEAEVKSIERFSW
                     SMAQAENQFELGQPMPPPFNKQSNEGGASLSADNRILFYTICKQEGGELLNCDLWYST
                     FGRDTWSEIKNAGPVINGKNTWESQPSLSSDGKTLYFASNRPGGIGELDIWKSVKDKD
                     GNWGAPINLGPEINTPGNEKSPFFHSDGQTLYFSSTGHLGYGGYDIFFAKLDKETGWQ
                     KPKNIGYPINSEKDDLGFFVSTDGKTGYFASDKLKGVGGWDVYAFGLYKEARPERVVF
                     LSGEIKSENNQSVADTRVEIQNIHTKEITTIDVDSVTGKYVAVMAFNEDHILTVKQPG
                     KAFTSEYLSVADSSLNKPRKLDVEVKEIQVGKAYKINNLNFNTNSADFNAQTVSIVLE
                     LAEFLKENPAIKIAIHGHTDNVGDPQTNMALSNDRARHVYELLLLEGIKSDRLTWKGF
                     GMTKPIASNLTEKGKALNRRTEFVITAK"
     gene            complement(25510..26433)
                     /locus_tag="PK281_00095"
     CDS             complement(25510..26433)
                     /locus_tag="PK281_00095"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HOY47762.1"
                     /translation="MKIVAKRIAQLKGHDGAVYTVNQAPESNAFLSAGSDKMVVEWSL
                     ETNKPIHVLARTPGVIYCTCLIPEYNLLLVGNDQGGIHVIDLVKKAEVRYLLGHTKGV
                     YSLSWNALTEQVYSSGGNGHWVSWSVHDFHAVKSMILSEKKIRTVAISENQKLVAIGT
                     GEGSIVLLNSSNLEVVHKWKAHEMSVNSLCFHPNGTSLLSGSKDAHLKSWDIEEDFRL
                     LVSIPAHNYAIYSIVFHPNKQLFATGSRDKTIKIWDANSCEFLLRIDAAQFQGHINSV
                     NSLHWSSHKNFLISGSDDRSVMVWEIGTGIE"
     gene            complement(26471..28210)
                     /locus_tag="PK281_00100"
     CDS             complement(26471..28210)
                     /locus_tag="PK281_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY47763.1"
                     /translation="MKNILTYFTFITLLVCTNQEIKSQTIEATYTPSFKVNDYQQLNI
                     LGKIDGKAVGRIENYKTMRNVYSKASYLVYLENLDNNNIITEKFTYPILPNQASTNNL
                     VLYDVFLMNDHLKFILGYDCEKEKENCVAIYSVEPSTLKPFDEDATSIDVFELDNSNQ
                     SEQANKQNITRYFKLSDDSSSLLVVSSYLSSENGINQSEIEKQYNINVREFDINSLDL
                     KSETELQTDVPVLNYELSQFNLTKSKQLILGFSRNPKFQIVSVDSEKIDIGPDKEDII
                     IYSGKVKSDKFERNGMNLFRPSRRLLEHHLQIDDSNIHLITLFRDTIREDKKVVMYIP
                     GYEIVTLNMEDLLETSSCELRPSPAQTAMCMPNKENITKQSSEMALATYYSRISDIKK
                     TDNGNWIICSSTDYGMDQTLFSGYILVARFDPKSKDNSWLRLVPKFQESTDGTLGLGY
                     SFEVLKEDVFLTMNDLQSNISVISDLDDLSINGKSLGESIPKINTKMIYKPYKRQPSN
                     VIRIDKLSEKGVISHSYIKVLPEIKNFLPTPTVSWTNNEGYLYILGLFEYDLMLDRFR
                     EFNFIKINLNTIK"
     gene            28334..30148
                     /gene="htpG"
                     /locus_tag="PK281_00105"
     CDS             28334..30148
                     /gene="htpG"
                     /locus_tag="PK281_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019670402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HOY47764.1"
                     /translation="MSTGKINVNTENIFPIIKKFLYSDHEIFLRELVSNAVDANMKLR
                     TLAATGEAKGELGELQIEVRIDSEKKTLTISDTGIGMTAEEIDKYINQIAFSGAEEFV
                     EKYKDKPEANAIIGHFGLGFYSSFMVASNVEIISKSYQEGAEAVRWECDGSPDFTLSA
                     AEKESRGTDIILHIAEDSLEFLETARIQTLLNKYCKFLPIPIQFNGEQINNVKPAWTQ
                     KPADLSDEDYLAFYKELYPYSEEPLFHIHLNVDYPFNLTGILYFPRLKKNFEIQKNKI
                     QLYSNQVFVTDSVEGIVPDFLTLLHGVLDSPDIPLNVSRSYLQSDGNVKKISSHITKK
                     VADKLEDLFKTNREDFEAKWDDIRMFIQYGMLSDEKFGDRALKFNLLKNVAEKCFTHE
                     EYLDHVCPLQTDKNEQVILLYAANKTEQHTFIDAAVQRGYDVLLMDSPLDSHLVNMLE
                     SKMEKVRFVRVDSDTVDKLIVKEDALPSKLSEEEQERLKPLIEKQVEGKMLSVQLESM
                     SESDAPVIVTRPEFMRRMKDMAQVGGGNPFMADMPDSANLVINTNHPLIGRILSTDDA
                     DKQANLIRQATDLALLAQNMLKGEELTRFIRRSVELID"
     gene            30252..31115
                     /locus_tag="PK281_00110"
     CDS             30252..31115
                     /locus_tag="PK281_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY47765.1"
                     /translation="MKHLVTCFLIVSGLSLSAQTPAADSKPTSNKRFSLSDYQLNVSF
                     NAYKPELTDYQTLKLIAANAADLNLPTNIDSFSRRPDSQEGSQGTVLVVLSASFSPYS
                     KKKEGYNKQQLWRVGLSFRDVNTYESSYNFAQTITQDSSTSRDYLVNANQKVLGLETM
                     YTFSTDVEKPVNVYVGLGLTAGYSISSNMEVNSNFNTIMKQPDGVNQTTFTPSSSIIK
                     GKNAMYMGVFIPAGFHVRVKKNIGVLAEFRYGMYSTTISSGPKFSRNSVFAGIGLRYT
                     LGVFEDKKPAY"
     gene            complement(31118..31357)
                     /locus_tag="PK281_00115"
     CDS             complement(31118..31357)
                     /locus_tag="PK281_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007647825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY47766.1"
                     /translation="MKSLRSVYLLIAIFFVAAQGPAMAQCAMCKATVESNIENGGKTV
                     GKGLNNGILYLMSIPYIVLGGIGFMVYRNWKGKST"
     gene            complement(31366..31908)
                     /locus_tag="PK281_00120"
     CDS             complement(31366..31908)
                     /locus_tag="PK281_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HOY47767.1"
                     /translation="MEVPVSKEKKFLPWIIAAGVAIPLVVGILAVAPKMEILGEFNYY
                     IFPLFNAIINGTTFLVLLLALYAIKQKNIALHKMLMTSAIALSLLFLVFYVLFHLSTE
                     TTKHPGSGTLLYVYYFVLASHILLSIAIIPLVLITYVRALSEKFDQHRKIARITLPLW
                     LYITLTGVIVYVMISPYYPQ"
     gene            complement(31908..32540)
                     /locus_tag="PK281_00125"
     CDS             complement(31908..32540)
                     /locus_tag="PK281_00125"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HOY47768.1"
                     /translation="MAYLVLTTGKHNIKKLGYFGTKIPGAKEGDTTYHSIPPFQFTDQ
                     DGKPFGDKDLQGKIYVANFFFTTCPSICPAMQTLMKKVQDTDDFKKLNDFRLVSFSVD
                     PDHDSVAVLKEYAQKIKADPGRWYFLTGDKKAIYDLAYQGFMVNAMEDPTAPGGFLHS
                     DMMLLIDRDKHIRGIYEGTSMKDVKRLIDEIKVLIAEYNSAKKENVNPIR"
     gene            complement(32587..32970)
                     /locus_tag="PK281_00130"
     CDS             complement(32587..32970)
                     /locus_tag="PK281_00130"
                     /inference="COORDINATES: protein motif:HMM:NF015582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HOY47769.1"
                     /translation="MEQTVDQSFEYSAGHGPRTPEQSRAVRKKILFVTLLLSVITIFE
                     VMVGIFYSQSHVSANTWTIIKWMYIVLTLIKAGYIVLVFMHLGDERKNLRTTILLPMV
                     LVVYLIFIALYEGEHLLQYFQDLMK"
     gene            complement(32991..33713)
                     /locus_tag="PK281_00135"
     CDS             complement(32991..33713)
                     /locus_tag="PK281_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HOY47770.1"
                     /translation="MAHSATIEIPSKQLWGGGTSPFGASYGKLMMWFFLLSDAFSFSS
                     LLIALGYARHVHADSWPLVEKVFHHFPFTHAHLPLLYVGMMTFILIISSVTMVLAVDA
                     GHTNNKNGVLFWLGLTVVGGLIFLGSQAWEWYTFIVGGEGVMKNVIVDGVPTDILVQG
                     ANLQYNEYGHTAFADFFFFITGFHGFHVFSGVVINAIIWKQAADGVFEKRGHYEMVEK
                     AGLYWHFVDLVWVFVFTFFYLL"
     gene            complement(33805..34392)
                     /locus_tag="PK281_00140"
     CDS             complement(33805..34392)
                     /locus_tag="PK281_00140"
                     /inference="COORDINATES: protein motif:HMM:NF012720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HOY47771.1"
                     /translation="MTTLSESNLEEKELRDKINRNLLYIGIFSSVMLFAGLTSAYIVR
                     QADGNWLQFTLPSMFWISTGVILASSASINWALIAAKNNRYSQVTQALGLTLLLGITF
                     CITQILGWQELTKGGVYFAGSSANPSGSFLYILSGVHLLHILSAILYAGLIFVKSLSQ
                     KYNSTNHAGVRHCGIFWHFLDGLWVYLFVFLLLMQ"
     gene            complement(34402..35295)
                     /gene="cyoE"
                     /locus_tag="PK281_00145"
     CDS             complement(34402..35295)
                     /gene="cyoE"
                     /locus_tag="PK281_00145"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013070105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HOY47772.1"
                     /translation="MAQDVSHTTESSAFASKVKDLMQLLKLRLASLVVFSAVVSYLMA
                     AENPDWTVAVLLTLGGFLVTGSSNAFNQIIEKDLDKLMARTENRPMPKERLTHTEALF
                     FSGLIGLIGVGILWYFINPLSGILGIAALVSYVAIYTPLKRITPFAVFVGAFPGAIPP
                     MLGWVAVSGEMSIQALILFGIQFMWQFPHFWALAWMLDDDYKKAGFHMLPASDGRSKT
                     SALQVMVYTASVIPFGLLPFVFKMAGPVSAVIITLTGIYFLMKAIKLHSTLEISDARK
                     LLYASFIYLPVVQLALLFDKL"
     gene            complement(35372..37009)
                     /locus_tag="PK281_00150"
     CDS             complement(35372..37009)
                     /locus_tag="PK281_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY47773.1"
                     /translation="MKAIPVCLFLLVWYTSVSAQQTNFVLDNFQYNLIDEAMNAKGLN
                     QHTTVKPWRMEDIRKIINPDTAAGVPIGKSRFSNRWIGRKLFRDNLFRLDSNDYQLAV
                     DPLFSFDIGKDQLTGKTTYQNSRGVQVFGNVGKKVSFYTSFWENQGSMVNYVDSFIQK
                     NHVVPGQGRVKPFGSNGWDYSMATGYVSYSPSKYFNFQFGNDKLFIGDGYRSLFLSDN
                     AYNQPYFRISTSFWKIRYTNLFVSYQNVGNASNSGIGGYLQKFATYHHLSLNATKWLN
                     IGLFESIIWQGGDTTGARRGFDVNYLNPIIFYRPVEFSRGSPDNVLIGLNFKAMILKK
                     YQLYGQILLDEFSLKEVKAGNGWWANKQAAQIGWKFYRFLGIKNLVWQNEFNLVRPYT
                     YGHFTGEQSYTHFAQPLAHPLGANFIENVQFLRYRYKRWGFQGKILMSQYGADTSSAS
                     GQTSNFGQNIFAGTAEIGSGPTMVPSIYGNYVLQGLKNTVLFTDLTVSWLVNTRTNMR
                     LELTFSRRMQENERITRNTNWFFVSFRTALPNRYYDF"
     gene            complement(37006..38040)
                     /locus_tag="PK281_00155"
     CDS             complement(37006..38040)
                     /locus_tag="PK281_00155"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HOY47774.1"
                     /translation="MEPFFAAFLFCLILTPAVIRFAATRRLFDIPDERKIHSAEIPRL
                     GGVAIMGALLISTLLIAGPDTLIHLRYFLAGLLILFFVGLWDDMQPVSPFIKLLGESI
                     PIVLMSWFNRMPLHELAPDIAWLPGFEFPVTLFLAFWIVNAFNLIDGINGLAGSIGMI
                     ALIGMGIMGEPDIYHLSFSFAGALLAFLFFNFLKPKIFMGDCGSLPIGYTLAFGMTQL
                     SFHNHCGTSTILPEEPLIAFSLMIVPLFDMVRVFFIRIRHQKNPFSGDRNHLHHLLLE
                     NGLSHVGATLQLVGLSLLSAGITTYLLTWCDKEVELVYILFLIPLPALLFTAVLWSRV
                     TKLRKQKNHL"
     gene            complement(38064..39035)
                     /locus_tag="PK281_00160"
     CDS             complement(38064..39035)
                     /locus_tag="PK281_00160"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOY47775.1"
                     /translation="MARLLITGGSGFIGRYFVERFKDREIVNLDLRAPDYTSHARYHA
                     GDIRNLNDVRNALQGVDMIIHLAAMHHDFGIADEAYFDTNVRGAEVLISEAEKAGVNT
                     IINFSSVAVYGGKGNPGPTNESTEPAPTNPYGVSKLEAEARFSKWANAAPGRKLIIVR
                     STVVFGPHNLANVLNLIKAIDEGLYFHVGKSNNIKSLAYVENIVDASLFALNKLNEGI
                     CLFNYVDEPQLSSREIANMQARFLGKKIRMILPRWMAMILAFPFDVAIKLSGKNLKIS
                     SKRVAKLITQTNHGADYIRELGFKPKYSIEFGMEKMIRWYKSTKKNR"
     gene            39094..40515
                     /locus_tag="PK281_00165"
     CDS             39094..40515
                     /locus_tag="PK281_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY47776.1"
                     /translation="MGSKNLLYYQSSILLILAIGILVVVNYFNQSTYDTGDSVMHFLY
                     ARHAMDHPMLYLEHWGKPLFTLFASPFAIFGWTGMKVFNLLVTLLSMFFLSATAEKLL
                     PNSRGAAALIFASAPQLLLMTASGLTEPLFGLLMSIAIWSESRKMYWLGAVIISLSLF
                     SRSEGQIILIIWLCWFVYQKRWKEIPLFFSAFIVYAIIGYFSKYHDFWWYFNKNPYEQ
                     PRFVYGSGPWNHFLVSFINITGPILLVLTFIGIIGFIYIVLPAFLIKKDKQKGVWVLA
                     FLSVAGFFFFHSYAWASGRFGSYGLLRVILGIYPLTVLLMVYAWQSLADKLNGNWKLI
                     VSNGVLLLALIYPFIGSKYSIHKVDLQPHPSLMQMQECLADMNQLTYPALVSAAPLPA
                     FVLGVDYFDDTKFRYLNTVELPYTQEGSIIVWDHHFAGSDLKLPANAFLQDTARFELI
                     KTCVPNEGQKGDSILVFKLLIAR"
     gene            complement(40493..41746)
                     /locus_tag="PK281_00170"
     CDS             complement(40493..41746)
                     /locus_tag="PK281_00170"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOY47777.1"
                     /translation="MLIRFIILFAVLLLIDIYFFQAVKTISSGWTDVRRMWTFRIYWG
                     FSAFSLLFGLFSMLTYQNPWFNKYIYLYVFCFIFIVVISKLIGSLFLMVDDLQRLVRW
                     VIGFFNQGVIPADGAKSGGISRAKFLSYTALTVAALPFTSMIYGMIKTAFDFTIRRNK
                     IPIANLPAGFEGLKIVQISDIHSGSFASDEPFKRAVEMIMSEKPDVIFFTGDLVNDKS
                     AEAERHIETWKKLSAPLGVYSILGNHDYGDYVEWDSREAKAANLQRLKEIHKEMGWDM
                     LNNEHRILERNGDQIGLLGVENWGEALRFPRKGDIVKAKAGMPEVPVKILLSHDPSHW
                     HAQVLKSHSDIDLTLSGHTHGFQFGIEIPGFKWSPSQYVYKQWAGLYSEGKQHIYVNR
                     GLGFLGYLGRVGIKPEITIIELSKA"
     gene            41745..42629
                     /locus_tag="PK281_00175"
     CDS             41745..42629
                     /locus_tag="PK281_00175"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOY47778.1"
                     /translation="MKVAKGSECGRVCQMACNSGNTSMQVPEQYTGEKVFAANIQDSK
                     LSVAKDTSLEDERRKVVFSAIFAGSLILVLWVVKIYEWIFDLDLHEFALRPRTLSGIP
                     GIFAEPLLHGDWSHLISNSVPLFLLLMATLYFYKGIGFKVLGYIWIITGVGVWLIARN
                     SYHIGASGIVYGLASFLALSGVLRNDTRLMSVSLLIIFLYGGMVWGVLPLFQHISWES
                     HLMGALAGIFCAIRYRKQGPPIRKYFEDEEEIPLSPSEEMGPFLPPDFHPGQPSQIHP
                     GGFYYRYVPKQPEEEAKP"
     gene            42639..44513
                     /locus_tag="PK281_00180"
     CDS             42639..44513
                     /locus_tag="PK281_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013551422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOY47779.1"
                     /translation="MNYLQVDRLSKSFGEKVLFENINFGLQQGQKTALVAKNGAGKST
                     LLRILMGKEIPDTGEVVFRNDCEVAYLDQHPPFEPEQTIVEAVFAWKDPRLEAIREYE
                     ELLETDPHSERLHEVIQKVDDLKAWDMEAKVKEILGKLDIHHLEQKMGQLSGGQRKRV
                     SLAALLIREPDLLLLDEPTNHLDVEMIEWLEGYLRQSNLTLLMVTHDRYFLDNVCTDI
                     LEIDRKSVLRYKGNYAYYLEKKAAAIEMFNSEVEKAKNTFTKELEWMRRMPKARGTKA
                     KSRIDSFYEVKEKASQRRNDDKVELSVKMNRLGGKILELIKVSKGYEGRTLIQPFTYT
                     FIKGEKIGIVGKNGCGKSTLLKLIMSQLEPDSGKIVVGETVIFGYYSQDGMKLAEDKR
                     VIEVVKDIGEYIPLANGTNLTASQLLQLFLFPPEIQYTYVSKLSGGEKRRLYLLTVLM
                     KNPNFLILDEPTNDLDIQTLNILQDFLENFSGCVLIVSHDRFFMDNLADHLFVFEGGG
                     VIRDYNGNYTDYRQEQAVLDKASKSEPAVVVPEVVVKQAAPKKEKSKPSFKEVHEHQQ
                     LEKEIPQLEERLANLTTKLNSGITDHVELQKIAQEITDLSNQIEEKSMRWLELSEIVG
                     "
     gene            complement(44510..44920)
                     /locus_tag="PK281_00185"
     CDS             complement(44510..44920)
                     /locus_tag="PK281_00185"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HOY47780.1"
                     /translation="MVEQYAAFWKRVVATFIDWILVSIITGLISWIITGEFAGDEAFR
                     KINTLFFFQVYGVYSILMESSRLGATVGKLALKIKICSVDHNQLSVPQAFFRYFARLF
                     SFFTAGFGCVMALFTLKRQGLHDQMTKTLVINKA"
     gene            45018..46055
                     /locus_tag="PK281_00190"
     CDS             45018..46055
                     /locus_tag="PK281_00190"
                     /inference="COORDINATES: protein motif:HMM:NF040069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6340 family protein"
                     /protein_id="HOY47781.1"
                     /translation="MKKLLPIVLFALFLSACQSTVSYTGLRPADVNLPTEIKSVVLVN
                     RYKADKKNSWINIVEGIFTGEVIFADKRGVEQALSGLQQRLQSGPKYTVTIANDQLTG
                     TGTGVLPPPLSQGDIQALCTQYNADAVITLEAFDSDIAVTHQQKINKRTENGKVIEDI
                     YFEAFENVRISMSWRLYLKSTGSIFDQHQMSTNRSFSSTGKTPDLARAALLFPVDAIM
                     QTGFQGGDSYGIRIAPSWVSYNRMIYTRAARRGGMKIAKRMAYRSEWAEAATIWEKLS
                     QSSNRKVAKRAMYNRAVAAEMLGNFDEALLWARKAANQHGLRAADNYIYQLNSRLNEL
                     QRLDQQMNPNP"
     gene            46061..46630
                     /locus_tag="PK281_00195"
     CDS             46061..46630
                     /locus_tag="PK281_00195"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOY47782.1"
                     /translation="MGKPVEPGYSVEQELSEIQASLKNPREFEKIYSRHFSTIYAYIK
                     SATGDKNLAADLCSDVFYSALINLAKYTTSEMGIRPWLFGIAINRLRMFFRQSGRMMY
                     VPIDDLKLQQLTEEDLSLEKKERRKQLSAYLSLLNEEEQEFVQLRFVAECSFREIALV
                     KGISEEACKMRLYRLLERMRKSMDKDTLL"
     gene            46627..46845
                     /locus_tag="PK281_00200"
     CDS             46627..46845
                     /locus_tag="PK281_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY47783.1"
                     /translation="MKEFNRKIQVTDSSQPDERELQEARDFSRIVTRLDEARKPLPKR
                     KLYQSKQRWFFLAILLIILILLILLEVF"
     gene            46907..47614
                     /locus_tag="PK281_00205"
     CDS             46907..47614
                     /locus_tag="PK281_00205"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOY47784.1"
                     /translation="MKKSPNSVLLACILYCMPFFVCAQPDIKDSRYLKSGFVHCTITS
                     MKGIPKQGETVVFEGEKSKKQVQSVSDSKGICDVWLPKGDVYLIKVLTVGKEEDYSSI
                     EVPDKPGAFRGNYSIQFDLPTTVTLDDVLFETGKSVLKPSSFPSLLKVAELLKHKKGL
                     VIEVVGHTDNRGDAAANMKLSLQRAQAVKAYLAEHSGAAERIKADGRGADEPIADNDS
                     EEGRKLNRRTEIHVLKE"
     gene            complement(47615..48586)
                     /locus_tag="PK281_00210"
     CDS             complement(47615..48586)
                     /locus_tag="PK281_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOY47785.1"
                     /translation="MATSTKATAKPNKVTKAVPAETKSAVSANGKADSNSTSSNRIKV
                     LKTYKIYIGGQFPRTESGRYYPLTLSDGEVVNICLSSRKDFRNAVVKARDAQDSWAGR
                     AALNRSQILYRIAEMLETRSAQFVEELIQQGSTKASAKAEVELSVDRLIYYAGWCDKY
                     NQVFSTVNPVSSSHFNFSSTESMGVVAMIAPQKSGLLGLISVIAPVIAGGNTCVVLAS
                     RDLPLCSVTFAEVLNSSDVPGGVVNILTGETKELLSHFSTHMDVNALYICTSDDAQKK
                     SAETNAALNVKRVRIEAEPNWSDAKQESPYRIMDYLEVKTTWHPIGI"
     gene            complement(48604..49464)
                     /locus_tag="PK281_00215"
     CDS             complement(48604..49464)
                     /locus_tag="PK281_00215"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOY47786.1"
                     /translation="MKLAEIIQESGLPAGVVNIVTGAGATGAAIVNHPGVDKIAFTGS
                     TDVGKIIQKAIAGTGKKLTLELGGKAANIIFEDAPLDQAVEGIVNGIFFNQGHVCCAG
                     SRLFVQEGVADKVIRKLKDRMESLIVGDPMDKNTDIGAINSKEQLKKINEYLKIGVNE
                     GAEIYQPNCSVPAKGYFCKPTLFLHTSQSHRVVQEEIFGPVLAVQTFRTVEEVIEKAN
                     NTPFGLSAGVWTDKGSKIFNMTTKLKAGVVWANTYNKFDPSSPFGGYKESGFGREGGL
                     HGLLPYLQFS"
     gene            complement(49470..50105)
                     /locus_tag="PK281_00220"
     CDS             complement(49470..50105)
                     /locus_tag="PK281_00220"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOY47787.1"
                     /translation="MSKKSHKSSKSADASELSLDFISKLAYAPAPESTGHIRLQKQYE
                     LFINGEWVKPVKGGYFETFSPSTEQSLARVAEATQEDVDKAVKAARNAYENVWSKMPA
                     KERGKYIYRIARLIQERAREFAVIETMDGGKSIRESRDIDVPLAANHFFYYAGWADKL
                     EYAFPGREVKSIGVAGQIIPWNFPLLMAAWKIAPALATGNTVVLKPAELLR"
     gene            complement(50132..51043)
                     /gene="deoC"
                     /locus_tag="PK281_00225"
     CDS             complement(50132..51043)
                     /gene="deoC"
                     /locus_tag="PK281_00225"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HOY47788.1"
                     /translation="MGTTIKSYNPALSPTIDQVGVEERCARFQTRSIKDETKIAGLKL
                     AMSMIDLTTLEGKDSVGKVQQMCYKAMHPHDAFPGIPQVAAVCVYPTMVKVARKALAG
                     SKIHVASVATAFPSGLAPLKVKLDDTRFAVEEGADEIDMVISRGKFLSGEFNFVFDEI
                     AAIKEACGKAHLKVILETGELSTLDNVRKASDLAILAGADFIKTSTGKIQPAATMPVT
                     LVMLEAIRDYYYATGKMIGMKPAGGIANSKLALHYLVMVKETLGADWLHPDWFRFGAS
                     KLTNDIIMQLVKQETGVYQSLNYFSND"
     gene            complement(51077..51433)
                     /locus_tag="PK281_00230"
     CDS             complement(51077..51433)
                     /locus_tag="PK281_00230"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HOY47789.1"
                     /translation="MRRYILPAIIWTIVVSTLTLLPGKDLPEIHVINFDKFAHLGVFG
                     LLNLLYLRWQRFGTNSGISSLWVTVFVVMYGGLIEFLQWAFYTDRTADFGDFAANTLG
                     CLLAWICLPLLPRKLR"
     gene            complement(51438..51818)
                     /gene="gcvH"
                     /locus_tag="PK281_00235"
     CDS             complement(51438..51818)
                     /gene="gcvH"
                     /locus_tag="PK281_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007647795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HOY47790.1"
                     /translation="MNFPDNLLYTKDHEWVRIEGDTAIIGITEFAQSELGDIVFVEVD
                     TVGETIEAEQVFGSVEAVKTVSDLYMPVAGEIIEFNNILDGSPETVNSDPYGDGWMIK
                     VKMSSPGDTSGLLSAADYKALIGH"
     gene            complement(51894..>52883)
                     /gene="sprA"
                     /locus_tag="PK281_00240"
     CDS             complement(51894..>52883)
                     /gene="sprA"
                     /locus_tag="PK281_00240"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HOY47791.1"
                     /translation="FQELSTLKTGTFSMSTNTWGTAFEKLDNKLHGSSNFQRFLDNRK
                     AVASRLTTNGIDPTTNYPTGFGSTQQDVLLYSFMSTYMGNDPTRQDLSLFPNIPKPNW
                     RITYDGLTKLEFFKKYFKTISLSHAYRSTFNIGSFTTNLLAIQDPNSTDQTGNIIPQL
                     NILMASLSEQFGPMVNVDMTWKNSLITKFEMRKTRDLSLSLQNSQITEVNGKEITIGS
                     GYIFNNVELPFKVVGSTKKIKSDLNVRCDVTIRTNKTVVRKIPIEGETLFDPILSAGQ
                     QNFTIKTTADYVVNSKFTIRLFFDRTSNKPFISNQFPNSNTSFGLSLRFTLNQ"
     assembly_gap    52884..52983
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<52984..>55643)
                     /gene="sprA"
                     /locus_tag="PK281_00245"
     CDS             complement(<52984..>55643)
                     /gene="sprA"
                     /locus_tag="PK281_00245"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HOY47792.1"
                     /translation="PLQDVGLDGLPNDSERVFFQDYLSEAQSVLNPDAYTQLQSDPSN
                     DDYRFFRSTEWDAQSADILTRYTLYNGLENNSRLGEDPIETYPTANNTIPNIEDINSD
                     NTLSTSEAYFQYRLSLRPQDMVVGQNYIIDKVPGKGELPNGKPIDVIWYQFRVPIRNY
                     SNKVGTISDFRSIRFMRMFFHNWQEQVVVRMARLELVRAEWRRFDYSLKEPGLFIGND
                     LNTQFQVSTVNIEENGDKTPVKYVLPPQIRREVDVTTTNLQQLNEQSLQMRICNLEDG
                     DARAVFKNTNLDIRAYRRIRMEVHAESMNNNQWPDKQLTAFIRLGTDFVNNYYEYEIP
                     LKRCADGTTNPEDIWNNSFNFLLETLTNAKIDRNTQMLNNPLITYSKPFYAQDPNQPE
                     NRITVVGVPQISGVKTMMIGIRNPQNDEYNPWPDDGSVGCAEVWVNELRVSDYFESGG
                     WAANARVTAKLADLGTIALAGSRTTFGFGSIEKKPADRARANTTSYSLISNLELGKFF
                     PKKWNLSIPLYWEFGENIANPQFDPLDADVKFERALDYQPSDGARDTLRQLSQVYQKR
                     KSVNFTNVKKNKAQGATKSHFYDVENLDFTYAFSDNFQRSPQVEFNYQKNIDVGLGYT
                     YAPQAKAWEPFKKAKIFEKTKAFALIKDFNIGLTPSRLAFRSDIARDFNSQKLRNTSD
                     IALLKIDTTFFKSFTWNRIYDIKYPLTKSLQLDFMATNQSRIDEEQGLINTSSKRDSV
                     VRNLKSGGRNTNYQHSLNASYTIPFSKVPILNWVSGNVSYAATYSWVAAPLVRDSSNL
                     NKFAANPFGNSITNSNSINSSAQANLMQLYNKVPYLKKINQKKGTKPKEKKKVKPLDP
                     KDPKAKKDSTEKKETAFSKILENAAKFLMML"
BASE COUNT        16591 a        11195 c        10926 g        16731 t
ORIGIN      
        1 tataataatt gccgctgtca agctcaaccg ctctggatgc attgcctgct aaattagccg
       61 tgtcattgta agtacgctca aaagttggaa cctgagctaa aagcgattga aatgaaataa
      121 gaagagcgaa tgcaaacaaa tttttcatag cgcctgtgat attttgcata tctattaact
      181 atttgtcctc gccacggaga tcaataaata cagtctttca ataattttcc gtgaaaaatt
      241 ctttttactc tcttctcact tctttctact ccttactctc tactctttac tctctacttt
      301 gtactttcta ctaatctaaa gatcccaaac ccgcgtcttg ctcgattgaa tcaagttctt
      361 gatgcgcaga ataaaggcca tgtgcaaata cacaataatg ggagaaccta aggtgaggaa
      421 ggaggcgtag atgaagaaca agcgaatttg tgatgacttc attcggagct tatcgcccag
      481 ccaagtgcaa acgccaaatg cttgtcgctc aaaaaaatcg gagatagact gaatcatggt
      541 ttggcgttgt tctatgtgtt ggctaatata gcgaaaagga tttctgatta caagcaagca
      601 aatcacattc agaaccgact ctttctctac ctttgcggtc caaaatcacg tcatgatcaa
      661 aggttttttt catctcccaa aagctgtaaa tgaaccggta aagagctatg ctcccggcag
      721 cgcagaaaaa acagaactga aagccatgtt gcaacatctg cagtcggtgc agctcgatat
      781 acccatggaa attggtggtc gttctgtatt caccgatcag aaaattgagt tgcgccaacc
      841 acataaacgt aagcatgtac ttggaacctt ttcgcgagga accgagcaac atgtgaaaga
      901 tgctatcgac gcggcattag ctgccaaagc caattgggaa aaaatgagct gggaaaaccg
      961 ttctgccatt tttctcaaag cggctgattt aattgcgggt ccttaccgtg cgcgtttgaa
     1021 tgcagctact atgttggggc aatcgaaaag tgcatatcaa gcagagatcg attcagcttg
     1081 cgaaatcatc gacttcttgc gctttaatgt gcagtacatg agtcagattt attctcaaca
     1141 acctgaatct tctgcaggaa tttggaaccg tgtggagcaa cgtcctttgg aaggatttgt
     1201 ctttgcgtta acgccattca acttcaccgc tattgcagga aatcttccaa cttctgctgc
     1261 catgatgggc aatgtggtgg tttggaaacc agccgatacg caaatttatg cagcacatgt
     1321 gttgatggaa attttcaaag aagcaggcgt gccagctggg gtaatcaacc tcgtttttgt
     1381 gagcggtcca acggctggaa atgtcatctt ctcgcatcct gattttgctg gtatccactt
     1441 taccggatca accggtgttt tccaaaacat ctggaaaacc attggagata atattcacaa
     1501 gtacaaaacc tatccacgca ttgttggcga aacaggcggg aaggatttta tcatggtgca
     1561 ttcttctgca caacctgctg tagtggctac tgccattgca cgtggtgcct tcgaatacca
     1621 aggacaaaaa tgttcagcgg cttctcgctg ctatgttccc gataatattt ggccagctgt
     1681 aaaagagcaa ttggttcgcg atatggcgag cttcaaaatg gggccagtag acgattttag
     1741 caatttcatc aatgcggtga tcgatgagaa atccttcgat aagttggctg cttatatcga
     1801 tgctgcgaag aacgatccga atgtcgaaat cgttgccggt ggaacctacg ataagagcga
     1861 aggctatttc attgctccaa ccgtcatctt ggcaaaagat ccgaagtaca cgactatgtg
     1921 tgaagagttg ttcggtcccg tggttactat atatgtatat gaagccgcta aattttccga
     1981 aactttaaag cttgtcgacg aaacttctat atacgctttg accggttcta ttatcagtca
     2041 ggaccgttat gctgtggacg aagctaccta tgcgcttcgc aatgctgcgg gtaatttcta
     2101 catcaacgat aagccaacag gagctgtcgt aggacaacag ccatttggtg gcgcacgcgg
     2161 ttctggtacg aacgataaag ctggctcgat gattaatttg ttacgttggg tttctccacg
     2221 tactataaaa gagaccttcg ttccaccaac cgattatcgc tatccatttt taaaggaaag
     2281 ctaaacgaat ccaatttttc aaacgcctca aaggactatt catttggggc gttttttttg
     2341 tgggcagctg aaccgggcta tacgtttcaa tcttttgctc gagggctcac aaaaggattt
     2401 tcactcctat ccctgctgcg ggtaatttcg atgtaagaaa tttattgcac tgattatgag
     2461 ctcaatacgc gcttgtaata atgccgctta atcctgcgtg tgattcaagt tgaaagagtc
     2521 gtgttttgcg attctcaacc aaccaaccat cacatgaaaa attatttact agcctctttg
     2581 gcttttcttt gcctgatatt gagcgtttcg gcaaaggacg gagaaggcag tcagctcttt
     2641 caaaatgttc aacgcaattt tgccagaacg gcaccacaaa gtttttcatt attcaaacca
     2701 tccaaccgag tgtatgttcc cggatcggtt aaaggaatcg agaatgctac attcttaact
     2761 atcgatcaaa atcctttcta tcaacttttt accactcaac ccgaaacagt aacccttcaa
     2821 gttcctgctc ccgatggttc tggtacatgg aaactagatt tgtatcgctc ccaaattctt
     2881 agcagcgatt tcgaagtgaa aacttccgat ggaaagaagt tctcagcacc tgaagcaatc
     2941 tattaccaag gaattttcaa taacgatcct caatcgatgg cggttgtatc ggtattcgaa
     3001 aaccaattaa tgggtatgtt gtcaaactca cacgggaatt acgatttagg tgtttatggc
     3061 gaagatcaac taagcgaata tgttttctac cgtagcaacg aagtcggtcg acagcttcct
     3121 ttcgattgtc atacgagcga tgtggacgaa cccgttgaaa cagctctagt agcgggcgat
     3181 tgtcgcgttg ttcgcgttta cttagaatgc gattttgatt tatacactaa gcgcacaagt
     3241 gtggcaaacg ttaccacatt cgtaaccgga ttttttaatc aagtagcagc aatttatacc
     3301 aacgaaaata tacaagttca aatatctcaa atctatgttt ggaccactac cgatccgttt
     3361 attagtcaaa ctacatcaag cggagtttta tctgcatttc gcacaacacg caccacattc
     3421 aatggtaaca tcgcccattt aatttctact cgatctacca atatgggagg tgtcgcatac
     3481 ctcgatgttt tgtgtgccac aacctatcgt cacgcattta gtaacatcta taattcgtac
     3541 ggtagttttc ctacctattc ttggacagta aactgtttct ctcacgaaat gggacataac
     3601 ttcggttcca atcatacgca atggtgcggc tggacaggtg gagcgctcga caattgttat
     3661 acaaccgaag gtggatgtgc agctggacca gcgcctgtga atggtggaac aattatgagt
     3721 tattgtcatg ttactggata cggagtaaat ctggcaaacg gttttggaac tcaaccagga
     3781 aataagattc gcaccaaata tttagcaagt acctgtctaa ctggcggtgt tgcaattagc
     3841 gttgtacctg caggagcgac gatttgctca ggttctaata tcactcttac tgcgtccgga
     3901 gcaacaagtt atacttggac gcctacaact ggattaaatg ttcccaatac agcttcggta
     3961 attgctacac catctgcaac aagtacatat actgccacgt cggtcgtgaa taattgtact
     4021 gtaactgcaa gcagtattgt aactgtttta gcttcagtaa atcgcggaac attagcaagc
     4081 ggaaatcaaa catttaccac atcgggcgat cctgctgcaa ttagtttttc aacagcagca
     4141 acgggaggtt caggaacatt tgcctaccaa tggtattcaa aagctggaat tcaaactgcg
     4201 ccaacaggaa catcaactac aggttggacg gccatttcag gagcaacatc ggctacctat
     4261 aatccaggag ttcaaactgc ttctatttca tacgctgtaa cagttgatgc aacaggaaca
     4321 gtagattgcg gtggagctgc atgggcaagt ggagttcgtc aaataacagt aaatgcttct
     4381 acaggcttta gtgctgggac tttgacaagt tctaatcaaa ctttttgcag tactggtgga
     4441 gatccagcga atatcatttt ttcggttgcc ccaagtggcg caacatcctt ttcgtaccaa
     4501 tggtattata aaaatgggat tgctgcagca ccaacaggta cttcaacaac cggctggacg
     4561 attatcaacg gagcaacagc cagcacgtat gatccagcat caggtttaac tcaaagcaga
     4621 acctacgcat gtagagttac gccttcaaca ggaacagcaa cttgggcaag tggagctcgt
     4681 caaataacta tacttccggc atttaatcct ggaaccgttg ctagtggcga tcaaagtttt
     4741 tgtgcaacag gtaatcctgg agcaattaat atgtctgcga atccggttgg atcgggcgct
     4801 tatacttggc gttggtatta taaagaaagc acttcgattg cttgtccgac aggtaattca
     4861 atcaccggat ggttgaccaa tacaaccagt gcaaacatta gcgggactac cttaactggc
     4921 gcaggagtta catttgatcc tatttcggct ggtgcaataa ataatggtcg gactttcgcc
     4981 gtgttaatta cacctattgc taatggaagt gttcctgcat gtggaacggc agcatgggca
     5041 agcaattgtc gtaaaacgta tgtgacaact tgtactggtg ctatgccagg tgaagaactg
     5101 gttgaaggca gccagcttga tttaggacag agttttccga atccaactaa ttcacattca
     5161 acaatccaat acagtttacc tgattcctat aatggagctc gattggtgtt ttacgatcag
     5221 ttaggtagaa ttatgtcgga acaagttgtc cctacaggaa tggcaaacga atatgagttt
     5281 aaaggagatt ttctcccttc aggaacctat tactacagtt tagaatttgc tggtactaaa
     5341 ctggcaacaa agaaattagt gctaatccac taatagttaa gtcaggtttt gggttaaaaa
     5401 aatcccggcc tttagagctt gatgctttaa ggtcgggatt ttagttttaa gtaattgcct
     5461 tagttttcgt ttggcaagaa agagtaacgt tttccgtaat ccaacatttg actagaaaat
     5521 gaagctggat attcaacttt cacatccaca aattttccgc cttcaattac tggaaccaag
     5581 cgcggttgga taaatccttt ataaggagca gaagctaatt tgctaaagcg atctttcact
     5641 tctttgtgaa tggcaggatc tactttaaca ccataatttt cgatcaaatc ctgtccagct
     5701 gcaaagtctc cttgcgattt tatacgttga atttcgcgaa gcaaatcacc aaatagcgca
     5761 cgtaatttta cgtaatcgtt gatttgaacg taggtttttc catcacgttt gatgagttca
     5821 actacgttgt tggctttccc tttttcataa acccatttag ccactaactg acgattacgc
     5881 atgtgtgctt cttcgatttc ttttccaatt tccacacgat ttaactgaag catcaaacca
     5941 tttagtaagt agctatcgta ttcggcttta ccgcaatcgg tgcttggcat aacaccaatt
     6001 tcaaccaatt tcggatcggt gatatagtat aatgctacta gatcggcacg ggcttcttct
     6061 agggcatttt ggtagttctt caaggtaata tctggagtag caacgccatc ttctaactga
     6121 ccgcttgcat gacctataac ttcgtgcata tcggtatgaa gctcagaagc tagattgcca
     6181 tatttacgag cacgttcctt tgtttcatca ttcaagctaa attcatccgt aaccggactt
     6241 ttcgcagaag caacattgta tgcgttaacg atatttccaa gagcaaccga tttagaacca
     6301 tgttccgcac gaatccagtc gctattggga aggttgattc caattggagt agaaggagaa
     6361 gaatcgccac tttcaacaat tacaacgatt gattttccga tgataccttt tacttctttc
     6421 tttttgtgtt tagggtccaa tggagaattg tcttcaaacc actgagcatt tttagcaatg
     6481 gtttcgattt tcttggtggc ttcgaaatct ttcaaagaaa ctaccgcctc aaaacttcca
     6541 cgtttaccta gtgcatcttg atacacctcg ataaagccat tcacattatc aatcgttgaa
     6601 ttgacgtcac ttacccaagc aatgttgtat tcattgaatt tcttgaggtc gcctgtttta
     6661 tagtattcca ccaaaagttc cagtgatttt ctttgttctg gagtttccgc aagaggaaca
     6721 gctttttcga gccagtaaac gattttttcg atcgaagcag aatacattcc accaaccttc
     6781 caaggaatct ccacgatttt accatctact ttcttgagtt tggaatttaa tccgtactca
     6841 attggagttt tatcaagtgg attactttta gcagcgtaat agtcttctac ttctttttgg
     6901 gtaacgcttt cgtagaagtt attggcagag ccagtaatgt tatcgattcc agatgctaag
     6961 ttcacggttt tcgcatccac agttggatcg aacatgacag gtttaaggaa ggcaagaaat
     7021 tgatcaacag tttgatcttt agcaagcggt aattgtttcg aatcggaagc tttcacccaa
     7081 gctgagaagc tttcgaacga aaatgttggg atgattttat cgctcgcata atggtgatga
     7141 attccgtttg agaaccaaac ttgtttcgag tattcttcaa acttcaggaa atcaggagtg
     7201 cttttaggtc ctttgtagct gttccaaata gcttcgattg tacgacgaac ttttaggttg
     7261 tttctatatt tctgatccca gatcatatct ctacccgaaa gtgctgcttc gtatagataa
     7321 tacgcgagtt ctttttgctt tatgtttaga ctttcgaaac ctgggatttg atagcgaagt
     7381 acttgcacat ctgcaaagcg atcggcttct actttgaatt cttcgggttt gctatcggga
     7441 cgaactttac ccggtttggt agtttgtgcc aggatgggtt gcgatgccgt tgtaaaggca
     7501 agtgcagcca tcagaataaa ggctttattc atgattgttt tattttagga aagagcgaca
     7561 aatttaaccg aatctgctta gcagcaatta ttttcgaaag gtgaaccaaa cggctaaaac
     7621 caaacagata aatgctgcta catgattcca atcgaatttc tccgttcgaa agacaaatac
     7681 agccatcaaa gcaaaaacag taagcgatat aacttcttga ataactttta gttgaatcaa
     7741 actaaatggt ccgccattac tttcgtgccc tatgcgattt gcaggaactt gaaatacgta
     7801 ttcaaaaaat gcaagtcccc agcttagcag aattatacct aataatcccc atttcattaa
     7861 gggagaagat tttttcatgt acaaatggcc ataccaagct agtgtcatga aaacattgga
     7921 aagtactagt agtccaagtg tgtaataccc gcgcattatg ctttttgtct tctacgtgct
     7981 ggtttaactg gagcaggagc gtttttccaa atgaaaatat cggaacgttt tgctgggcga
     8041 tgttctccta accagcgcaa tccacgcaat tggatttcct ctttggttgg ttcattcacc
     8101 ggataaagag tcgcatttgg atcgctaata aatgtgatgc tttgcacttt gttactatcg
     8161 atataaatat tcatatcgtt acaaattgcc ttattcacgc ccatgtatcc atctccatca
     8221 ccttgagcat aatagaccga ttctccatta gcgaccactt ctattttcac caattcattc
     8281 ttcacaaaat aaccagtcac atttctaccg ctaatctggt taaaatggac actatcttcc
     8341 tgcgaagcaa taaatccatt cgttcgtaaa taaagtgttt ccagattatt gttagcaaat
     8401 tgaatcctta ctgtatcggc tgttagctga ttttcttcgt tccaaataac tggatcttta
     8461 tacaatcgca tcatgctatc tagttccgaa taggtaagcg aatcgcagcg accttgaaaa
     8521 tcctttttga aaaatttcac tttgtgatac gcaaacaaag ttcgtttgtc ggtaaccgta
     8581 tctaaaaccg aacgcaaggt gtcgccatga agaaataagg tgtctttgcc aaattccttt
     8641 tccatcacag cgctgtcggt cataagaatt cgtcctgtaa attcattata atcagcaaaa
     8701 tgtccagtga gaacaacagc ttgcgatgta tcacttatac gaacattttg tcgcgcacgt
     8761 ccatcaccag ttttacggtt ataaaaaagc ttctcggctc taatttgttg tttcttacta
     8821 tcgagtcgag cgttctttcc aaattctgaa atatcggttt tcgtgttgta gtatccgttt
     8881 tcgcaataaa tcaggttgtt cttggaagtg atcgtagttg gaccaaaaaa gtaggacgtt
     8941 ttatttaccg ttgaatactg caacgtatca cacaccatca cataatcttt gttggtgagc
     9001 tttacatttt tacgaaagta gaatgttcga ttggtgctat tatacatccc aaactgacta
     9061 accaactcgt ttttctcgct gatgattttt ccacctgttg tgtatcgagc ttgcttggtg
     9121 ttattatcga attgcaaacg gttggtggtt aaaaccgagg atggatcagt cattctcaca
     9181 tttcccacga tatcagctat tttggttttg ccatcgtagg tgagattttg tccggttact
     9241 accacgctat caccaacaat tcgcacattg gagtaggctt ctaagcggtt ttctttatcg
     9301 tagagataag cactatcgca atacagcaaa gtgccatcgt gttcgaaaat cacattccca
     9361 acaagacgtt gcgcatcaac acggtcgtcg aattcgagtg tattcgcgct tttgatatta
     9421 atttttctac ccgtttgcgc cgaaagcata ctcgtgctga gcagcatgag catgagaaga
     9481 acaggaaatt ctccccggaa aaaacgagca aatgaatcat tcataagtat cgcgcaaatg
     9541 tacaggatat taaacgattg aaaagagcgt ttcgcatcta tatcaatggc tgtttctacc
     9601 tgcattttcg agagtgaatg ccaaaaacac taagtttgcc acctaatttt agctcatgag
     9661 tttccctgtt acgcgcgtta ctgaatcccg tttgccatct atcaatctgg atcatcttcc
     9721 atttggaaag attttttctg atcatatgtt tatcgcagag tatgatggga aaaattggat
     9781 taacccacgc atcgagcctt atgctcccat ttctttaagt ccggctgctt ctgtatttca
     9841 ctacggacaa gccattttcg aaggaatgaa agtgtttcgc agtgacaagg acgagctttt
     9901 atttttccgc ccggaggaga attacaaacg catgcagcaa tcggcgattc gcttatgtat
     9961 gccacctgtt cctgaacata ttttcatgga tggtttgcgt caattggttg atttagatgc
    10021 tgcctgggtt cctaaagggg atgaaaactc actttatgtt cgccccgttt tatttgctac
    10081 tgaaccagtt ttaggggtaa agccatcaga aacatattct tttattattc tcacttctcc
    10141 aacaggtgct tattatacag aggatttgaa ggtggtgatt gagaaaacat attcacgtgc
    10201 aagccgcagc gggactggtt ttgccaaagc tgctggaaat tatgcaggtg ctttatatcc
    10261 aactcaactc gctcagcaaa aaggctacaa tcaagtaatc tggaccgatg ccgctacaaa
    10321 taccaaagta gaagaatcgg gtacaatgaa cttgatgttt gtgattaacg gagtgttgat
    10381 tacaccatcg ttgagtccga gtaaactggc tggaattacc cgtgatagcg taattgaatt
    10441 agcgcgtcat tggggaatgt tggtgcaaga acgtgatgtt tcggtgagtg agattattgg
    10501 tgctttccgc gctggaacgt tagaagaagc tttcggaact ggaacagctg ctaacattgc
    10561 acatatctca gtcattggtc acgaaggaga agacttcaaa gtgccagaat tacatgctga
    10621 tttgttttcc gttaaagctt tgagattctt aaacgatctg aaagcaggtc gcacagctga
    10681 tccatttggt tggatcacga aactctaatt attaacgtaa gcgaatggtt tgttcgcgat
    10741 ctggtccaac agaaacaatc gttaccggaa cttctagtgc ttgttcaata taacggatgt
    10801 aggcttttaa gctttctggg aattgatcaa gggaacgaat tccagtaagg tcttcattcc
    10861 atcctggcaa gtcggtgaaa actggctgaa cgtaacgtgg atcaatgtcg aaagggaatt
    10921 catcggtttc cactccatcc actagatatg aagtacaaac tttcaccgtc tcaaagccac
    10981 tcagcacatc ggctttggtc atgatcaact gcgttacacc gttgattaga atcgaatatt
    11041 tcaaagctgg taaatctaac caaccacagc ggcgcggacg accggttgtt gctccatatt
    11101 cgcgacctat ctgacggatt ttctcacccg tttcattttc taattcggta ggaaatggtc
    11161 cacttccaac gcgtgtacaa tatgctttga aaataccaag cacttcacca attctacccg
    11221 gtgcaacacc taaacctgtg catgctccgg cactgatagt atttgaggaa gttacaaatg
    11281 gatacgaacc gaaatcgatg tcgagtaggg aaccttgtgc accttcggct agtacttttt
    11341 taccggcttt aagttgatga ttgaggatgt gttcgctatc gatgtgttta aaaccacgca
    11401 gatgttcgat tccttcaaaa aatgctggtt cgagttcgga taaatcgtag ttaaactgat
    11461 aatgagcgag catttcctcg tgcttctgaa ctaagaaacg gtagcgttcc atgaaatcag
    11521 gacgcatgat atcgcccaca cgaagtccgt tacgaccggt tttatccatg taagttggac
    11581 caataccttt tagcgttgat ccaatttttg atgttccttt tgaagcttcc gaagcggcat
    11641 caagcaaacg atgagtagga agaatcaaat gagctttacg agaaaggtgc aggtttttat
    11701 gtgcatccac cccaagttta ctcaaagcat caatttcttt tttgaaaatc actgggtcga
    11761 taacaacacc gttacccacg atattcatac attcgggatg aaaaataccg gatggaatgg
    11821 tatgtaaaac gtgcttgatt ccgttgaatt cgagcgagtg accggcattt ggaccacctt
    11881 gaaaacgagc aattacttcg tattcgggtg ataaaacatc aacaatttta ccttttcctt
    11941 catcgcccca ttgcaggccc aacagaatat caagcttcat ttttttgttc agattttccg
    12001 acagaaagca cttgaatggc ttcgtcggta gttttagttt gttgtataac tgcgtcgagt
    12061 ttggtaatca ctagcagtcg gagaagttta tcgttaatgt tgcacataat cacttctcca
    12121 ccggcattcc gagcacgggt gaaaatattg atcaaaacat ttaatccact actgttgagg
    12181 taatcgagtg cttgtaaatc taaaatgatt cgtgctttat tctcagcaaa taactcggca
    12241 gtttcatcta gcagttcttg tgcttcatgt ctttccatca agctaccttc gaggctaaat
    12301 accggaaatg ctagcgattc atctagctca aacgaaaatg ccatcaggat ttttgattag
    12361 ggtgattttc acaaaaacct ttctccgaca gcttattaca ctttccataa aaagttagcg
    12421 agtgactgct aatgttaaaa ttgagcaatt cgcctgtcat actttgaatt tgttgaatcc
    12481 ttggatcaca aaactcaaat accttatcac aatcggagca aattaggtga tcgtgttgct
    12541 tgtatttata tgatttctca aactgtgctt ggtttttacc aaattgatgg cgagtgacca
    12601 agttgcaagt gagcaataat tcaatggtat tgtacaaagt agcgcgacta acacggtact
    12661 tcttattttt catgtacacg tacaaggact cgatatcgaa gtgtccgttg tgggaataaa
    12721 tctcatccag gattgcaaat cgttcgggtg ttttacggtg cccgttttgt tccagataat
    12781 cagagaaaat ctgtttgacg gtatcttttg tatcctggtc tgacatatta attcgaacag
    12841 gcgcaaaagt agtaaaaagc cagtcttacg cgataaatta caagctttct ttcaatcagt
    12901 tggaatcaat tcgtacaaca gaggttacgc cctttacctg cttgattttt cccatcaaag
    12961 aagtaaggtg attcgtgtcg tgaacgaata ccatgatatt accttcgaac acaccatcga
    13021 gcgaatcgaa acttatcgaa cgcatattca cgtttagctc attggaaatg attcgggtga
    13081 tgttatttac aattcccaca tcatcaattc cattcacgcg aataccagcc ataaatgcta
    13141 attgctgctg attcatccag cgcgctttca caattcggta agcgtaattc gacattaaac
    13201 taatcgcatt cgggcaattg gttttatgaa ttttgatgcc ttcgttgatg gtaacaaaac
    13261 caaatacttc atcacctgga attggattgc aacatgttgc caatttgtaa tcgatgcgat
    13321 ccatatcctc gccaataacc agcatgtcgg ttttgccgcg gatgtcttta acgatatttt
    13381 caagcgtaac gggttgatct tctttgctgt cggaatcgct tcgcttaaac ttatcccgaa
    13441 gtactttata ccagctatta tgttctcctt tagcaatctt ggcaatgttt tccagtttga
    13501 tgattccctc accaatgctg taaaataaat caagctgact atttactttg aagtgcttca
    13561 acaaattgtt gatgatattc ccatcgagcg caatcttgtt ggctttgagc tttttctcta
    13621 aaatgtcttt tccttcatca gctactttcc gtttttcttc tttaagggct gattttattt
    13681 tcgaacgagc ctttgctgtg acaacaaagt tgagccagtc ttctttcggt ttttgtttac
    13741 tagaactgag aatttcgatc tgatcaccac ttttcaactt gtggctaagt ggaacgagtt
    13801 tattattcac cttcgctcca atacacgcca ttcctacttc ggagtgaatg tcaaaagcaa
    13861 aatcgagcgc tgatgaacca cttggaagcg ttttcaattc tcctttagga gtaaatacaa
    13921 atatttcttc cgagaacaga ttgagtttga agtcgttaat gaactccaaa gcattggtat
    13981 cggaattttc caacatctca cgaatacgtc ctaaccactc atcaattcca ctttcgtaat
    14041 cgttgcgttc tttatatttc cagtgtgcag catatccgcg ctcagcaata tcatccattc
    14101 tgcgcgaacg aatctgtact tctacccatt taccagtcgg gctcatcaca gttgtatgca
    14161 aggattcgta accgtttgca cgcggggtag aaatccagtc acgcaaacga tctggacttg
    14221 gttgatagat atcggttatg atggaatata cacgccagca atcggctttt tcggtttcaa
    14281 ccggagaatc gataatgata cgaatggcga agatgtcgaa gatttcctca aagggaattc
    14341 ccttaatctt aatcttattg tagatggaat aaatcgattt ggggcgacct ttaatatcga
    14401 atgtgaatcc ttgctccgtt agactattcc gaatcgggtt gacgaannnn nnnnnnnnnn
    14461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14521 nnnnnnnnnn nnnnnnnnnn nnnnnnaata ttttcctgaa attctcagct tggatggatg
    14581 agctttggtc aaagacattc tcaattttag taagtccgtc gatgattttg gcttcagttt
    14641 caccaaacat ggagcggata tcttcgagcg taacgtcggt atcttcaact gtatcgtgaa
    14701 gtagggcaca aataacggct gtagctccca atcccatttc acttacagca atttgagcaa
    14761 cagcaattgg gtgatagata tacggctcac ccgattttcg acgcatgtcc ttgtgggcat
    14821 ccagcgctaa atcgaaagct ttccgaattt gttgtttttc gtgtttatcg atcttatcag
    14881 ctaaaggacg cagcaaagcc gtgtaccttt taagtatctc tttcttttct ttttccagat
    14941 cgatcacagg ggcagacatg ttcattcaga tatactgtaa aaatagggaa cacgagtgct
    15001 ctacatcaag cacctaaaaa aattaccagc ggtagtatgt tgaagaaaaa aattacttcg
    15061 agatgaccgt tgtcgttggt cgttcctgat aatttcgagt tggttcttct ttccaatcac
    15121 ttttgtcaga caagtactga tagatatcga tttgagctct gccgcgttct ttagcaacag
    15181 atttacgata cgtattgttc tgaattttat tgataatccg agctttggta ttatcatacc
    15241 attgaagacg gaaaatctcc aatacttcgt tgcgcaaatc ttcctgatag attgggaaac
    15301 ctacttcgaa acggcgactt aaattacgtt ccatccagtc ggccgacgat agatatacct
    15361 taggtttatt ttcgttgcag aagatgtaaa tcctggaatg ttccagaaag cgatcgacaa
    15421 tgctaatgac tttgatgttt tcgcttaagc ctttaattcc gggaattaaa caacatatcc
    15481 cacgaataat caatcgtatt tccacacctt cctgacttgc ttcgtagagc ttatctatga
    15541 tagcagcgtc ttgaagactg ttgagtttta gatagatata tgcgtctttt ttcttcaacg
    15601 cattttttat ttcctgatca ataagcgtgt ataaatcgtg acgcatgtga tcgggtgcca
    15661 aaagaaggtg cttgaattcc cgtttgatgc gtgtatcagc taaaatatca aacaccgcag
    15721 aaacctcgcg ccccatgtat ttatctttcg taaacaaaga agaatcacaa tacaagcgag
    15781 aagtattttc atggaaattt cccgtgctta ggtaaacata ccgtctgagt ttatcttcca
    15841 tccgttcgat acaaaccatt ttagcgtgca ctttaagaat tggaaaacta tacagcactt
    15901 taactccagc ctcttctaat tctttggaat aactgatgtt cgattcttca tcaaatcgag
    15961 cttttaattc cacaaatgca gtaacgtttt taccgttctg agcagccttg ataagtgctt
    16021 caacaacctt tgaatgagga gcaactcgat acaaagtaat tttgattgcc tttactttta
    16081 catcttcagc cgcttcattc aaaaaacgaa tcacgtaatc atacgaatga tagggaaaat
    16141 ggatgaggaa atcacgtttg ctaattgcct caaacatgga tggcgattca tccaaggttt
    16201 ttttcttcaa gcaaggcaga ttctcgtaat aaattccctt ttgatcgaat cgagggaaat
    16261 tcattaaatc ggagaaattg tgatagcgtc cgccaggcaa taaatcctga ttaccgatgc
    16321 taaatatttt tttcaaatgc gacaagaaat ctgccggcat ggcactgtca taaagcaatc
    16381 gagtaggtgt gcccttcgca cgattgctaa gtcctcgctt gattttcttg agcaaacttc
    16441 cagagaattc atcttctaac atcatttccg catcgcgcgt caatttgatg ctgtatgcgt
    16501 cgatcaattc ataatcggga aataattcgt tgatgcataa acgaatcaca tcacctaaaa
    16561 tcatcacata atgttgtccg tttttatcag gtagaattaa aaaacggcta atttttctgg
    16621 atggaatttc agtgatagca tagtcgtaaa cgcgcggacc gggacgatcg aaatcgcaga
    16681 gttttacggc tagatagata gatttttcgt ccaactcagg tgtgttgcga cgcgtattta
    16741 tcatcactgg acgaatatga ggaagaacct tttcgcgaaa atattcttgt acaaaaacaa
    16801 cttgttcggg agttaaattt ttctcgttta ctaagttgat attgcgtcct tctaattcaa
    16861 caactatctg attccagaat atatcgttga attcgttgag gtgttttttt actgttttat
    16921 ttatcttctt aatgacttct cttggatctt cccgaagttc ctttttggtc ttcttggtca
    16981 gttccattag tcgcttccat tcagctaccc gaatgcggta aaattcctcc agatttgaag
    17041 agaaaattgc taaaaatttc atgcgctcat aaagtggcac acgtaaatct ttagcttcct
    17101 gtaatacccg ttgattaaat gaaagccagc ttaaatcacg attaaatgta cgcttaacac
    17161 tcattttatt gtttcttttt cggaaaatct aaatcttcga gttttcccat ccctttttcc
    17221 catccgttcc aacgattact atttacacga attcgagcaa cagcacaagt tggaatttgc
    17281 gcttcttcgg ttccacatag ccaattaata taatcgctaa cacccggatt atgcccaacc
    17341 aatagtattg tatctatatc aggatcttgg cgtgaaatca aacgagataa atcattccgt
    17401 tctgcttcgt agatagaaga atcttgtttg attttttctt cctgaagttt aaaagcaatg
    17461 gccattaaac gtgcagtgct gatagctcgc ttagccggac tacttaacag aagttgaggt
    17521 gcagcaccat tcgtaagaat acgaacagcc atttcagggg cgttttttat tcctcgatct
    17581 gttaaaggcc gatcaaaatc ttttaactct tcctgctccc agctagattt ggcatggcgt
    17641 acaatgtaga gtgttttcat ctgtatcttt aatgtgactg caatgttaag tataaattaa
    17701 gccttgcttg gttggtattg aaaaatttaa cattggaatt atttcttgtt ttgccttttt
    17761 cgggttttag ttttctctac ttgttcattc gaatcacttc ctgatggaac aaatgtttta
    17821 gagctttcat cccagcgttc tattttacgt gtgatagcat gaattctaaa acgaaaacga
    17881 tcattccaag gatctttctt agagcgaaaa acggcaatct tataaggatt ttcgcgagtt
    17941 ggttctccgc ttaaatacat acgtagtcgt aaatcttctc gatcagtcgt atttaaatcc
    18001 tgccatgtag gattctcttt cagcgactta tcaaaccaag taatttcagg caaagagtat
    18061 agtgtgtcgg aaattaaatg gaatggaaaa aacttaaatg tctctggcaa tccacctgat
    18121 tgtgcttcac gaattaattc ggcacatagc tgttttatct ctgaactagg agcattgaaa
    18181 ttattcagga gcaccgaaaa agctattttt ctatcattta catctttcaa ataacctgca
    18241 tatcctctta caccacctat actacccgat tttgctcgca cattcggaat ttccggaaag
    18301 gatttcatgg aaccactttt tcctgcttct ggtatcgttt taatgaaatc ttcaaatcct
    18361 gattgagatg ctacagaacg tagaaaactg gtttgaaaag cagtactaat gtggtttgta
    18421 cgggaaagtc cgctggcatc ttgcaaaatc attagactat cggataaaaa caaggaattc
    18481 tttagataac cacgaatggc ttgagctcca tcttcagcac gagtgcctat tttagcttgc
    18541 tttcctaaca tttttccaac tgtttcagca aacaaattat gactcgtatg attaatttca
    18601 tttacaatat tcaataaagg tggtgacgca atcactccaa gcgtatcaca attgactaat
    18661 gacgaatttt gaataccggc aacttgactt ttaccactaa ttcgtatccc tttatttttc
    18721 aaataggaat ctaattcacc aataaattga gcaggcggat taggaataga accacgttct
    18781 gtttgacttt caccttttgg tttttgaaca ccatccaaat aaatgataga actcaaagga
    18841 gccgaataca cataaatttc ttcaggagta tttccagctt ttattgcatt acggaattca
    18901 acattatata actctggaat tggtcgagaa tacttggaga tagcaccaat cgtatcgccc
    18961 ggactaaaac tcatttggaa actattttct ctccaattta atccatacga acctgcaccg
    19021 taataatttc ctatgtcttc ccaaggccag ttgcgaggaa ctgcagtatg atcaaatcga
    19081 aaaaaatatg gatcaataat taaattccct tttatttttc ggataccttt ttcgtgtaaa
    19141 atctgataga ttttttctag aatattttca gcagtcattg cacctgcttt cccgctacct
    19201 aaagttggat caccaccacc aatcacacat aaatctcctc gaagaacatc atcttttatt
    19261 tcaccctgaa tgacgatttg ggttttgaaa gtgaaatcgg ctcctaatat tttcaatgca
    19321 gcagctgtgc tgaataattt ggctgttgat gccggcatca gatacgtttg ctcttggtaa
    19381 gtacttaaaa tctgccccgt ttctacatcc cgaaaacacc aacttaccac agctcctttt
    19441 gttgcattcg attgcatcat ttttcgagtg gcttcagtaa aagaattcga ctgtgcttga
    19501 atgtcaaaac gattgctata aaccaaacat atcagcagaa tggtgattcg ggtaaacact
    19561 ttcatcttcc aaaagtaagt tttcaaacga ttcttcccca atacaagcta cgaaggctta
    19621 tttttgcccc atggctggaa actcccacgg tgttttattt cgatttagta gcttcgggga
    19681 atcgcacgga ttagcgattg gtgggcttat tgacggatgt cctgccggtt tgaaactcga
    19741 tttgctagcc atacaacatc aactcgatcg tcgtcgtcct ggacaatcga atatcagcac
    19801 tagtcgaaaa gaatcagaca cagttcaatt tttatcgggc atttttgagg agaaaaccac
    19861 cggagctcca attggattca ccattccaaa ttctgatcag cattcgagcg attacgatgc
    19921 gttgaaagat gtatatcgcc cttcacatgc cgattatacc tatcaagcca aatatggaat
    19981 tcgggatcat cgtggtggtg gacgatcatc agctcgcgaa acggcaacac gcgtggttgc
    20041 tggctctgtt gctcgtcagt tattagccct tcagcacatt agaattgttg catttgtaag
    20101 tcaagtgggt gaggtgattc ttcctttgcc tacaactatt ccaagtgaga aacaggttga
    20161 tgaaagtacc gttcgttgtc ccgatttaga atttgcaaag tcgatggaag ccttgatttt
    20221 gcaagcgaaa gaagaaggcg attcgcttgg tggcgtgata acatgtatga ttgctggagt
    20281 tcctgcaggt tggggcgaac cggttttcga taaacttcat gctgatttag gaaaagcaat
    20341 gttgagtatc aatgcggtga agggatttga gattggtagt ggcttttcgg ctgctgccat
    20401 gcgaggttcg gttcataacg atgcttttat ccaaaacgaa cagggcgaga tccgaactat
    20461 aactaatcac tctggaggta ttcaaggtgg aatttccaat ggagaactca tctactttcg
    20521 agtagcattt aaacctgttg cttcgattcg aatatctcaa gaaaccgttt cccaagaaac
    20581 tggaaacact aaaattgaaa ttcctggtcg ccacgatcct tgtgttgttc cgcgtgctgt
    20641 tcctattgtg gaagcgatgg ctgcacttgt acttgccgac cattacctac gatctaaagc
    20701 aataaattca aacctttaag gttaattctt cagctcatct cctatgctca aaaagattct
    20761 taaaatcacc ggaattactt ttcttcttct ctttattatg gctttgattt taccttttct
    20821 gtttaaggat aaaatcattg caaaagtgaa ggaggaagcg aacaagaatc tcaatgctaa
    20881 gctcgaattc cgcgatttaa gtttagggct aatcagcagc tttcctaatt tcaatatcga
    20941 attacaagga ttgtcattat ctggaataaa tgagttcaaa tccgacactc ttatttcggc
    21001 tgaatcactt gctatcacta ccgatatcat gagtgttatt ggtggaggag aaattgccat
    21061 taaatccatc gatgtagata aagctcgaat tcacctgaaa gttttaaaag acggaaaagc
    21121 caattgggac attacaaaac catcagctac gcccgaaact ccatctacag aaccttctac
    21181 tttcaaagct aaattgcaag agtattcctt aaaatcgagc cgtatttggt atgaagatca
    21241 aacaatggat tttaccgctt tgcttgatgg aataaatcac tctggatcgg gcgattttac
    21301 tgccgatgtg actaatctag aaaccctaac tacaatcgat tcgatggatg tagtttatgg
    21361 aggaatagct tatttgaaac gtgccaattt agagtacaaa gccgatttca atctcgacct
    21421 aaaaaactcg gtttatgcct ttaaggataa tgaactccga ttgaataatc ttttactcaa
    21481 atttgccggt tctattgcaa tgccggccaa tgatatcacc atggatatca cgtatgaagc
    21541 attgaaaaat gatgtaaaga attttatttc tatcttcccg ggtgctttta cgaaagattt
    21601 tgaacaggtt aaatcatctg ggaagcttgc attcaatggg tttgtaaaag gcgtttataa
    21661 tgagaaaagt attccagggt ttgcctttaa tctgctcatc gaaaacggat caattcaata
    21721 tccatcatta cctcgaggta tttctaattt gcaagtgaaa acttcgatta cttgtccggg
    21781 tgtggatgtc aacaaaaccg tgattgatgt atctaaactt cacgccgatt tagggcaatt
    21841 tccaattgat gcgcacatga ttgttaaaac acctatttct gatccggata ttgatgcagc
    21901 aatcaaaggc aaggttgaat tggggacatt gaaagatatg ttaccacttg aagcaggtac
    21961 aaatttaact ggtgaagttg ctgctgatgt tagcttaaaa ggccatatgt cagctgttga
    22021 gagtggtaaa tatgagcaat ttgctgcagc tggaactatg tctgtagctc gattccgtta
    22081 tgctgcaaag gatttacctc aggaaattca aattaatcaa gcggcgctta atttcaatcc
    22141 taaagctgca cagctaagtc aatttgacat gcaattgggt aagagcgata ttcacgcaac
    22201 aggaagcctc gagaattatt tagcttatgt tttccgggat gaagcaatta ctggaaatct
    22261 atctctaacg agtaattttc ttgatctaaa cccattcatg actaccgaaa ccggaacaac
    22321 acctccacct agcaatgctt ctcagcctgc tgatgtgaat gggtatatca gagttcccca
    22381 aaacattgga tttaccttga attcatccat aaaaaccttg ctgtatgata acatgcgctt
    22441 ggaaaacgta agcggacaat tattggtgaa agatcaaagt gtacgattga atcaattaaa
    22501 aatgaatacg ctaggtggtg caataaccat gggaggttta tacgatacgc aaagcgaaac
    22561 tggaccatcc gtagcgctca tgcttgatat gagtaatttg aatattcgtg aaactgctaa
    22621 aacatttaat acagtaaaac aactggctcc gattgcagag aatacaaccg gaagtgcatc
    22681 tattaaaaat ttcaatttct cttgtaaagc tgataaagca tttaatccag atttgaaatc
    22741 cattaatgga agtggaactt tgagtacatc agcactcgaa attgaaggtt ttgaaatggt
    22801 gaaacgcatt gcaggagcct tgaaaatcga caagcttaaa aaatggaaaa tggagcctgt
    22861 gaatgctgga tttactatta ccaacgggaa agtaacgctc aagccattta aaactaaaat
    22921 tggaaattat gctaccgaaa ttggtggaga gaatggatta gatcaaacaa tcaattacgc
    22981 tgttcatatc gatatacctc gtgctgaatt tggtggtgct gcgaattcgg tgttaaatgg
    23041 tttggtttca aaagcgaata gtaacggact tcaaactcaa gttggagata tgattccagt
    23101 tgcgatatta gtgaccggaa cttttaaaga tccaaaagta aaaaccgaca ttcggaatca
    23161 agcaaataat gcgatgaatg acttgaaaaa tcaagcagag cagcgtttga aagatgaggc
    23221 agaacgtcag aaaaaagaat tgcttgataa agcaaatgct gaaaaagagc gtttgaaaaa
    23281 cgaggctgag aataaaatta atcaagaaaa agaaaaagct aaacaagaat tagataaagc
    23341 aaaatcagaa gctgaacgta aagcgaaaga agaggccgac aaagccaaga aaaaagcgga
    23401 agaagaagcg aagaaaaatc tgaataaatt attaaaaggc aagtaatacg tgagttttag
    23461 aagatttcta attgtatggc tcttacttct tcctgcgcta agtttattgg cacaggaaaa
    23521 agaagatcct tgcccattat cttccaataa aaaggcacgc aaactttacg aaaaagctat
    23581 tgatgccgca cgttctaata aaaatgaagc gagaagtatc ttaacagaag ctctcgaact
    23641 cgatcccgaa tttggcagag ctaactacat gatggccgat ttactcatca aggctaaaaa
    23701 aagtagcgaa gcggagccat ttttaaagca agtagctgct atatgtcccg atttggatcc
    23761 gcttactttt tatcgtttag gtagtattca atttggtgct aaacgatacc aagaagctaa
    23821 aacgaatctt tctcgctttc tggaatcgaa tggtggaaag gaaaaagatc gtagcgatgc
    23881 acgcgaacaa ctcactgccg caactttcta cctcgaaggt tttgctaagc ctgtaccttt
    23941 tgatcccaaa cccttggcat ccatttcatc cgaagccgat gaatatctac cggttattac
    24001 tgccgataat caacgtgctt actttacccg ccgaaccatg gaaaaggcaa agtttaccgg
    24061 tggaatagaa gcggaagtga aatccattga gcgttttagc tggagcatgg cacaagccga
    24121 aaatcagttt gaactgggtc aacctatgcc tccacctttc aataaacaat caaacgaagg
    24181 aggagcaagc ctatcagccg ataaccgaat tctattctat accatttgta agcaagaagg
    24241 cggggaattg ctcaattgtg atttgtggta ttctacgttc ggtcgtgata cttggtcgga
    24301 gattaagaat gctggaccag tcattaatgg caagaacact tgggaatctc aaccatcgct
    24361 atcctctgat ggaaaaacgc tctatttcgc tagcaatcgt cccggtggaa taggggagtt
    24421 ggacatttgg aaatctgtaa aagataaaga tgggaattgg ggtgcaccta tcaatcttgg
    24481 accagaaatt aacacacccg gaaacgagaa atctccattt ttccattccg atggacaaac
    24541 cttatatttc tcttcaaccg gacatttagg atatggtggt tacgacattt ttttcgcgaa
    24601 gcttgataaa gaaaccggat ggcaaaaacc taaaaacatt ggttatccga tcaattcgga
    24661 gaaagacgat ttaggctttt ttgttagcac ggatgggaaa accggatatt ttgcttccga
    24721 taagctcaaa ggagttggtg gctgggatgt gtatgcattt ggattatata aagaagcccg
    24781 tcctgagcgc gttgtattcc tttctggaga aataaaaagt gagaacaatc agtcggttgc
    24841 tgatacacgg gttgagattc aaaacatcca caccaaagaa atcaccacaa tcgatgtaga
    24901 ttcagttaca ggaaagtatg tggcggtaat ggcttttaat gaagatcata ttttaacagt
    24961 taaacaaccg ggaaaagcat ttacgagtga atatttatct gttgctgatt cttcgctgaa
    25021 caaaccacgc aagctggatg tagaagtgaa agaaattcaa gtggggaagg catacaaaat
    25081 caacaacctg aactttaaca ccaattcagc tgatttcaat gcacaaacgg tgagtatcgt
    25141 tctcgaatta gccgagtttt tgaaagaaaa tccagcaatt aaaatcgcca tccatggaca
    25201 caccgataat gtaggcgatc cgcaaaccaa tatggcttta tcgaacgacc gcgcccgcca
    25261 tgtatacgaa ctgctacttt tagaaggcat caaatctgat cgccttactt ggaaaggctt
    25321 cggcatgacc aaacccattg catctaacct aaccgaaaag ggtaaagctt taaatcgcag
    25381 gacggagttt gtgattacag caaagtagat agtattaagt aaagtgtatt aagtattaag
    25441 atttatagag aatgctagtt taaagcaatt cgtaatactc tgtactctgt actttttact
    25501 aagtactttc tactctatac ctgttccaat ttcccacacc atcaccgaac gatcatcact
    25561 tcccgaaatc aggaagtttt tatgagatga ccaatgcagc gaattgaccg aatttatgtg
    25621 tccttgaaat tgggcagcat cgatgcgaag tagaaattca caggaattcg catcccaaat
    25681 tttgatggtt ttatctcggc tgccagttgc gaatagttgc ttgtttggat ggaaaacaat
    25741 ggaataaata gcatagttat gagctggaat gctcactagt aaacggaaat cttcttcaat
    25801 atcccagctt ttcaaatgtg catcttttga accgctcaat aagcttgtac cattgggatg
    25861 aaagcaaagt gaatttaccg acatctcgtg tgctttccac ttatgtacaa cttctagatt
    25921 gctactgttt aacagtacaa tagaaccttc accagttcca atggcaacta atttttgatt
    25981 ttccgaaata gctaccgttc tgattttctt ttccgaaaga atcatcgact tcactgcatg
    26041 aaaatcatgc acactccaac taacccaatg tccattacca ccactactat aaacctgttc
    26101 tgtgagtgca ttccaggaaa gcgaatacac accttttgta tgaccaagca gatagcgcac
    26161 ttcagctttt tttactaaat caattacatg tattccccct tggtcattcc caactaaaag
    26221 caggttgtat tcaggaatta aacaggtgca ataaattact cccggagttc gggctagcac
    26281 atgaatgggc ttatttgtct caagcgacca ttcaactacc attttgtcgc ttcctgctga
    26341 taaaaaagca ttgctttcag gcgcttgatt gacagtataa acagcaccat catgaccttt
    26401 taattgagcg attcgtttag caacgatttt catatagcaa aggtaagagc aaatgcaagt
    26461 ttgatatcct ttacttaatt gtattcagat ttattttaat gaaattaaat tctctaaaac
    26521 gatctaacat taaatcgtac tcaaatagtc cgagtatgta taggtatcct tcattatttg
    26581 tccaagatac agttggtgtt ggcaggaaat ttttaatctc aggtagtact ttaatataag
    26641 aatgagaaat cactcctttt tcggataact tatcaatacg tatcacatta gaaggctgtc
    26701 tcttataagg tttataaatc attttggtat ttattttagg aatggattct cctaaagatt
    26761 ttccatttat tgataaatca tctaaatcag atataacact tatgttcgat tgtaagtcat
    26821 tcattgttag gaatacatct tcttttagaa cctcaaatga atatcctaat cccaatgtgc
    26881 catcagtact ttcttgaaat ttgggtacca atcttaacca tgaattatct ttcgatttgg
    26941 ggtcaaatcg tgcaactaaa atatatccag aaaatagagt ttggtccatt ccataatcgg
    27001 ttgaactaca tattatccaa ttgccattat ctgttttttt tatgtcagag atcctggaat
    27061 aatatgttgc taatgccatt tctgaacttt gcttggtaat gttttccttg ttaggcatgc
    27121 acattgcagt ttgagcaggg cttggtcgta attcacaaga agaagtttcc aacaaatctt
    27181 ccatgtttaa agtgactatt tcataacccg gaatatacat tactactttc ttgtcttctc
    27241 gaattgtatc tctgaataag gttattaaat ggatattcga atcatctatt tgcaaatgat
    27301 gttcaagcaa ccttctgcta gggcggaata aattcattcc gtttctttcg aatttatctg
    27361 atttgacttt tcctgagtaa ataataatat cttctttgtc aggaccaata tcaatttttt
    27421 cagaatccac gcttacaatt tgaaatttcg gatttctcga aaatccaaga atcaactgtt
    27481 ttgatttagt taaattgaat tgtgacaatt catagttcaa aactggtaca tctgtttgga
    27541 gttccgtttc tgattttaaa tccagagaat taatatcaaa ttctcgaaca ttaatattat
    27601 actgtttttc aatttcactt tgattaattc cattttctga tgacaaatat gaactaacga
    27661 caagcaagga agagctgtca tcactgagtt taaaatatct cgttatattt tgcttattag
    27721 cttgttcaga ttgatttgaa ttgtctaatt cgaaaacatc aattgatgtt gcgtcttcat
    27781 caaatggttt aagtgtggaa ggttctactg aataaatagc gacacaattt tccttttctt
    27841 tttcacagtc atatccaaga ataaatttta aatgatcatt catcaaaaag acatcatata
    27901 agactagatt atttgttgac gcttgattag gtaaaattgg ataggtgaat ttttcagtta
    27961 tgatattatt attatctaaa ttttctaagt atacgaggta agaagcttta ctataaacat
    28021 tcctcatagt cttataattt tcaatacgac caacggcttt cccgtctatt tttcctaaaa
    28081 tatttagttg ttgataatca tttaccttaa atgaaggcgt ataagttgct tcaattgttt
    28141 gcgatttaat ttcttggtta gtacaaacta gaagtgtaat aaaagtgaaa tacgttaaaa
    28201 tatttttcat ccgatagtaa tttctcaaat ataacttctc gtcattaata gcgcaaaata
    28261 tttgtcattt tgtcaccttt ctcttactgg cacttacttt gaaaacggcg gtagtcctaa
    28321 ttaaaattga attatgtcaa ccggaaaaat caacgtaaac accgaaaaca ttttccctat
    28381 tattaagaaa ttcctgtatt cagatcatga gatctttttg cgggaattag tttccaacgc
    28441 agtcgatgct aacatgaaac tgcgcaccct tgctgcaacc ggagaagcca aaggtgaatt
    28501 gggagaactt caaattgaag tacgtatcga ttcagaaaag aaaacactta ccatttcgga
    28561 caccggtatc ggtatgactg ccgaagaaat tgataaatac atcaatcaaa ttgctttttc
    28621 aggcgcagaa gaatttgtag aaaaatacaa agacaaacct gaagcaaatg ccataatcgg
    28681 acattttgga cttggatttt attcttcgtt catggttgca tccaatgtag aaattatttc
    28741 caaatcctat caagaaggag ctgaagctgt tcgttgggaa tgtgatggaa gtcccgactt
    28801 tacactatct gctgctgaaa aagaaagtcg cggaacggat atcattcttc acattgctga
    28861 agattcgctc gagttcttgg aaactgctcg tattcaaaca ctcttgaata aatattgcaa
    28921 gtttctacct attccaatcc agttcaacgg cgagcaaatt aataatgtaa aaccagcttg
    28981 gactcagaaa cctgccgatt tgagcgatga agattattta gctttttaca aagagcttta
    29041 tccgtattca gaagagcctt tattccacat tcatctcaat gtagattacc catttaactt
    29101 gactgggata ttgtatttcc cacgtttgaa gaagaacttc gagatacaga aaaacaaaat
    29161 tcaactttat agcaatcagg tttttgtgac cgattcggtt gaaggaattg tacccgattt
    29221 cctcacttta ttgcatggtg tattagattc acctgatatt cctttgaacg tttctcgttc
    29281 ttatttgcag agcgatggaa atgtgaagaa aatcagcagc cacatcacta aaaaagtagc
    29341 cgataaactt gaggatttat tcaaaacaaa tcgcgaagat ttcgaagcta aatgggatga
    29401 tatccgcatg ttcatccaat acggaatgct tagtgatgag aaatttggag atcgtgcact
    29461 gaaatttaat ttgcttaaga atgtagctga aaaatgcttc acacatgaag aatacctcga
    29521 tcatgtttgt cctttgcaaa ccgataaaaa cgaacaagtc attcttctat atgctgccaa
    29581 caaaaccgaa caacacactt ttattgatgc tgctgttcag cgtggatatg atgtgttgtt
    29641 aatggatagt ccgcttgatt cgcatttggt gaatatgctt gaaagcaaaa tggaaaaagt
    29701 tcgatttgtt cgagttgatt ccgatactgt tgacaaacta atagtaaaag aggatgcgct
    29761 tccttcaaaa ttatcagaag aagaacaaga gcgtttaaaa ccactaattg agaaacaggt
    29821 ggaaggtaaa atgctttctg tacaattaga atcaatgagt gaatcggatg cacctgtgat
    29881 tgtgactcgt cccgaattca tgcgacgtat gaaagacatg gcacaagttg gaggtggaaa
    29941 tccatttatg gccgatatgc ctgacagtgc aaacttggtc ataaatacca accatccact
    30001 aattggtcga attttatcta ccgatgatgc tgataagcaa gcaaatctca tccgccaggc
    30061 taccgatctt gccttgttag ctcaaaatat gctgaaaggt gaagagctca ctcggtttat
    30121 ccggcgaagc gtcgaactaa tcgactaatc ttaaactttc tcaggccctg aatttctcga
    30181 ttcagggctt tctttttttt ctgccgcggt gtgtatcaga tttttggtat tttgcctctt
    30241 caaccagcgc tatgaaacac cttgttactt gttttttgat tgtaagtgga ttaagccttt
    30301 cagcacaaac tcctgctgca gattctaaac ccacatccaa taaacgattc agcttatccg
    30361 attatcaatt aaatgtatcg ttcaatgctt acaagcctga attgaccgac tatcaaactt
    30421 tgaaattgat tgctgcaaac gctgcggatt taaatctacc aacaaacatc gattcgttta
    30481 gtcgccgccc cgatagccaa gaaggaagtc aaggaacagt tttggttgtt ttatctgctt
    30541 cattttcgcc ttacagtaag aaaaaagaag gatacaacaa gcaacaatta tggcgagttg
    30601 ggctttcatt tcgcgatgtg aatacgtatg aaagctctta caattttgct caaacgataa
    30661 ctcaagatag cagcacttca cgcgattatt tggtaaatgc taaccaaaaa gtacttggac
    30721 ttgaaacgat gtacacgttt agtacagatg ttgaaaagcc cgttaatgta tacgttggat
    30781 taggattaac tgcaggatat tcgatttcat ctaacatgga ggtgaattcg aatttcaata
    30841 ccatcatgaa acagcctgat ggtgtgaatc aaaccacttt caccccaagc agttcgatta
    30901 taaaaggaaa aaacgccatg tacatgggag tatttattcc agcaggcttt cacgtgagag
    30961 tgaagaaaaa tattggagtg ttagctgaat ttagatatgg tatgtattcc actacaattt
    31021 ctagtggtcc taaattctca agaaattctg tatttgctgg gattggactt cgttatacat
    31081 taggtgtttt cgaagataaa aaaccagctt actaagatta agtgctttta cctttccagt
    31141 tgcggtaaac cataaaccca attccaccta aaacgatata tggaatagac attaggtaaa
    31201 gaatgccatt attaaggcct tttcctactg ttttaccacc gttttcgatg ttcgactcaa
    31261 ctgttgcttt acacattgca cattgagcca ttgctggacc ttgtgcagct acaaaaaaga
    31321 ttgcaatcaa caggtaaaca gaacgcaggc ttttcatcgt gtattttatt gaggataata
    31381 aggcgaaatc atcacgtaaa caattacccc ggtaagagtg atgtataacc acaaaggtag
    31441 ggtgatacga gcaattttgc gatgttggtc gaatttctct gaaagcgctc gtacataggt
    31501 gatgagaaca agcggaataa ttgcaatcga aagaagtatg tgagaagcta agacgaagta
    31561 atagacatat agcaaagttc cactacccgg atgtttggtt gtttcggtag aaaggtgaaa
    31621 caagacataa aagaccaaga acaaaagcga taaggcaatt gctgaggtca tcaacatttt
    31681 gtgaagggca atattttttt gtttgattgc gtaaagtgcc aaaagaagaa caagaaatgt
    31741 tgttccattt ataatggcat tgaaaagagg gaatatgtaa taattgaatt cccctaaaat
    31801 ttccatttta ggagcaaccg ccaaaatccc aacgaccaat ggaattgcta ctcctgcagc
    31861 tataatccaa ggtaaaaact tcttttcttt tgaaacagga acttccatta tcgaatcgga
    31921 ttcacatttt ctttcttggc agaattgtat tcagcaatca agacttttat ttcatcaatt
    31981 aaacgcttca cgtctttcat actcgtgcct tcataaattc cacgaatatg tttatcgcga
    32041 tcgattaaca acatcatatc gctatgtaaa aagccacctg gtgccgttgg gtcttccatg
    32101 gcatttacca tgaaaccttg gtaggctaaa tcataaatgg cttttttatc acccgttaaa
    32161 aagtaccagc gacctggatc tgccttaatc ttttgagcat attcttttag aacagccacc
    32221 gaatcgtgat ccggatcaac cgaaaaagaa accaatcgaa aatcgttcag ctttttgaaa
    32281 tcatccgtgt cttgaacctt tttcattaag gtttgcatag ccggacaaat actagggcaa
    32341 gtggtaaaaa agaaattggc gacatagatc ttgccttgaa gatctttgtc gccaaaaggt
    32401 tttccatcct gatcggtaaa ctgaaaaggg ggaatagaat ggtaagttgt atctccttct
    32461 ttggcgccgg ggattttggt tccaaaatat cccagtttct tgatgttatg ttttcctgtt
    32521 gtgagaacca ggtatgccac ggatggcact aacaagatca tcagaaggat gacagctttt
    32581 ttcactttat ttcattaagt cctgaaagta ttgaagaaga tgttctcctt catatagtgc
    32641 aataaaaatc aagtaaacaa ctaacaccat aggcagtaga attgttgtac gaagattttt
    32701 acgttcatca cccaagtgca taaatactag gacaatgtat cctgctttaa tcaaagtaag
    32761 aacaatgtac atccacttga tgattgtcca tgtattagca gatacatgtg attgagaata
    32821 gaaaatccca accattactt cgaaaatggt gataaccgaa agaagtaagg taacgaaaag
    32881 tatttttttg cgaactgcgc gactttgctc tggtgtacga ggaccgtggc cggcgctgta
    32941 ttcgaatgat tgatcaacag tttgttccat agtagtgttg aagttttaga tcagagcagg
    33001 tagaagaagg taaatacgaa aacccataca aggtctacaa agtgccagta tagaccagct
    33061 ttttcgacca tttcataatg accgcgtttt tcgaaaactc cgtcggcagc ttgtttccaa
    33121 atgatggcat taattactac tcctgaaaat acgtggaagc cgtggaatcc agtaataaag
    33181 aagaagaaat cggcaaaagc tgtatggcca tattcgttgt attgaagatt agctccttga
    33241 acaagaatgt cggtaggaac accatcaaca attacatttt tcatcacacc ttcaccccca
    33301 acgatgaatg tgtaccactc ccaagcttga gaaccaagga atattaaacc tccaacaaca
    33361 gtaagaccta accagaataa tacaccgttt ttgttattgg tatgcccagc atctactgcc
    33421 aataccattg taacactgga gataatcaag ataaaggtca tcatcccaac atacaacaac
    33481 ggaagatgag catgtgtgaa cgggaagtga tggaaaactt tctctaccaa aggccagcta
    33541 tcggcatgca cgtgacgtgc gtagcctagc gcaattagca gtgacgaaaa ggaaaaggca
    33601 tctgatagta ggaaaaacca catcatcagt tttccgtaac tagcgccaaa tggagaagtt
    33661 ccacctcccc aaagttgttt tgatggaatt tcgattgttg ctgagtgagc catatcttga
    33721 ttcagaattt tgctgcaagt ttacccaaaa atttcatttg ggaattgcct ttacgcagga
    33781 aatcgtgttc tgttttaatt ttttttattg catcaggagt aaaaacacaa aaagatagac
    33841 ccaaagtcca tccaagaaat gccagaaaat cccgcaatga cgcactccag catgatttgt
    33901 tgagttgtat ttttgactta atgatttgac gaaaattaat ccggcataaa gtatcgcaga
    33961 aagtatatga agcaaatgaa ctcccgaaag gatgtataaa aagcttccag atggatttgc
    34021 cgagcttccc gcaaagtaaa ctccgccttt agtcaattct tgccaaccta gaatttgagt
    34081 gatgcagaat gttattccta gcaataaggt caagcctaaa gcctgagtaa cttgactata
    34141 acgattgttc ttggcagcaa ttaatgccca attgatagaa gcactggaag ctaaaatcac
    34201 tcctgtacta atccaaaaca tggaaggaag tgtgaactgt aaccaattgc catctgcttg
    34261 gcgaacaata taggctgatg ttagtccagc aaatagcatc acactactga aaatacctat
    34321 ataaagcagg tttctgttta ttttatcccg aagctctttt tcttctagat tggattccga
    34381 tagagttgtc atcgaacgta attaaagttt gtcgaacagt aatgctaact gaacaacagg
    34441 gagatagata aaggatgcat acaggagttt ccgtgcatcg ctaatttcta atgttgaatg
    34501 aagcttgatg gctttcatta aaaagtaaat tccagtcagt gtgatgataa ccgccgaaac
    34561 tggaccagcc attttaaaga caaaaggaag taatccaaat gggatcacac tagctgtata
    34621 caccattact tgcaaagccg atgttttaga acgaccatcg gatgcaggta acatgtgaaa
    34681 accagctttt ttgtagtcat catccaacat ccaagctagc gcccagaaat gtggaaactg
    34741 ccacatgaac tgaatcccaa ataatatcaa agcttgaatg ctcatttcac ccgaaacggc
    34801 aacccatcct aacataggag gaattgctcc tggaaatgct cctacaaaaa ctgcaaatgg
    34861 tgtaattcgc ttaagcggag tgtaaatagc aacataagaa accaatgcag ctatacccaa
    34921 aataccactt aaaggattga tgaaatacca caaaattcca acacctatca atccgattaa
    34981 tcccgaaaag aataatgctt cggtatgtgt taatcgctct ttgggcatag gacgattctc
    35041 agttcttgcc atgagtttat ccaaatcttt ctcgatgatc tggttaaatg cattggatga
    35101 accggtaacg agaaatcccc ctaaggtgag taaaacagct acagtccaat caggattttc
    35161 tgcggccatc agataggaaa ccacagccga aaacacaact aatgaagcta gtctgagctt
    35221 tagtaattgc atcaaatctt tcactttaga agcaaaagca gatgattcgg tagtatggga
    35281 aacgtcttga gccaacacct aattttagaa ttgcggcaaa aataggtaaa acctatgctc
    35341 tgcaaacgta ttgttccttt atttgttcaa ttcaaaaatc ataataccga ttcggtaagg
    35401 ctgttcgaaa actcacaaaa aaccaattgg tatttcgagt gatacgctca ttctcctgca
    35461 ttctcctcga aaaagttagt tccaaacgca tattggttcg agtattcact aaccaagaaa
    35521 cagtcaaatc ggtaaagaga acggtgtttt tcaatccttg aaggacataa tttccataaa
    35581 tcgaaggaac cattgtaggt ccgcttccaa tttccgctgt gcctgcaaaa atgttctgtc
    35641 caaaattgga tgtttgccca gatgctgaac ttgtatcagc cccatattgc gacatcaaaa
    35701 tcttcccctg aaaaccccaa cgtttatatc gatatcttaa aaactgaacg ttttcgatga
    35761 agttggcacc tagtggatga gctaatggtt gagcaaagtg cgtatagctt tgttctcctg
    35821 taaagtgacc atatgtatat ggacgaacga gattgaattc gttttgccaa accaagtttt
    35881 taattcccaa gaatcgatag aatttccatc caatttgagc cgcttgttta ttagcccacc
    35941 atccgtttcc ggcttttact tctttcaacg aaaactcatc taataatatt tgtccgtata
    36001 gctggtactt tttcaaaatc atagctttga aattcaatcc tatcaagaca ttatcgggtg
    36061 atccgcgtga aaattcaact ggacgataaa agataatagg gttgaggtaa ttcacatcga
    36121 aacctcttcg agcaccagta gtatcgccac cttgccaaat aatcgattcg aacaatccga
    36181 tattcaacca tttagtagca tttaaactta aatgatgata ggtggcaaac ttctgtaaat
    36241 atccaccaat gccactattg gaggcatttc ctacgttttg gtagctgaca aataaattgg
    36301 tataccgaat cttccaaaag ctggtgctta ttcggaaata aggctggttg taggcattgt
    36361 cggaaagaaa caaagaacgg tatccatcac caataaacag cttatcattc ccaaattgaa
    36421 aattgaagta ttttgatggt gaatacgaaa cataaccagt tgccatcgaa taatcccagc
    36481 cattgctacc aaagggtttt acgcggcctt gtccgggtac tacatgattc ttttgaataa
    36541 aactatccac ataattcacc atactacctt ggttttccca aaagctggta tagaaagaga
    36601 ctttcttacc cacattcccg aaaacttgta caccgcgtga gttttggtaa gtagtttttc
    36661 cggtaagctg atcttttccg atatcgaacg aaaacaaggg atcaactgct aactgataat
    36721 cattcgaatc taaacggaac agattatctc gaaacagttt tcgtccaatc caacgattcg
    36781 aaaaacgtga tttgccaata ggaacacctg ctgccgtatc aggattaatg attttacgga
    36841 tatcctccat ccgccaaggt tttaccgtag tatgttggtt caagcctttg gcattcatgg
    36901 cttcatcaat caaattgtat tgaaagttat ccaacacaaa attggtttgc tgcgccgaaa
    36961 cggaggtata ccaaaccaac aagaatagac aaaccggaat agccttcata ggtgattttt
    37021 ttgttttctg agcttggtta cccgtgacca aaggactgcc gtgaaaagta aagcgggtag
    37081 gggaataagg aatagaatgt aaactaactc aacttcttta tcacaccaag ttagtaagta
    37141 tgtggtgatt ccagcactta gcaaggaaag tccaactaat tgcaaagtag cacccacatg
    37201 acttaatcca ttttctaaga gcaaatgatg caggtgattt cgatctccgc taaatggatt
    37261 tttttgatga cggatgcgta taaagaagac ccgtaccata tcgaaaagcg gcacaatcat
    37321 taaggaaaaa gcgatcaaag gttcttcggg cagaatggtg cttgttccgc agtgattatg
    37381 gaaactcaat tgcgtcatcc caaaagctaa ggtgtaaccg atgggcaatg agccgcaatc
    37441 gcccatgaaa attttgggtt ttaggaaatt gaagaataga aaagccaata atgctccggc
    37501 aaacgaaaaa cttaagtgat agatatcggg ttcgcccatg attcccatgc ctatcaaagc
    37561 tatcattcca atacttcctg ccaagccgtt gatgccatca atgagattaa atgcattgac
    37621 gatccaaaaa gctagaaata gcgttacagg aaattcaaaa ccgggtaacc aagcaatatc
    37681 gggtgctaat tcatgaagtg gcatgcggtt aaaccacgac atgagtacaa ttggaatact
    37741 ctcgcccaat aatttgataa atgggctaac cggttgcata tcatcccata atccaacgaa
    37801 aaaaaggatt agtaatccgg caagaaaata gcgaagatga attaacgtat ctgggccggc
    37861 aataagcaaa gtgctgatga gcaatgcgcc catgattgct accccaccta atcggggaat
    37921 ttcagcagag tgaatcttac gttcgtcggg aatatcaaat aacctgcggg tagctgcaaa
    37981 acgaatgaca gcaggggtaa gaattaagca aaatagaaaa gctgcaaaaa agggctccac
    38041 gctcggggtt gttccgggcg aaattaacgg tttttctttg tgctcttata ccagcgtatc
    38101 atcttctcca ttccaaactc aatactatac tttggtttga agcccaattc acggatgtaa
    38161 tctgcaccat gattggtttg ggtaattaat ttagcaacac gtttggatga gattttcagg
    38221 tttttaccgc tcagtttaat cgccacatca aagggaaatg ccaaaatcat cgccatccaa
    38281 cgtggtaaaa tcattcggat tttcttgcct aagaatcgtg cttgcatgtt tgcaatttct
    38341 cgactactca gttgaggctc atctacataa ttgaacaaac aaatgccttc atttaattta
    38401 ttaagagcga aaaggcttgc atccacaatg ttctccacgt atgcaagtga ttttatatta
    38461 ttcgatttac ctacatgaaa atacaaacct tcatcaattg ctttaatcaa attgagcacg
    38521 tttgccaaat tgtgcggtcc aaaaaccact gtcgaacgca cgataatgag ttttctaccg
    38581 ggagctgcat ttgcccattt actaaaacgg gcttctgctt ctaacttaga aacaccatat
    38641 ggattggtag gagctggttc ggttgattcg tttgttgggc ctggattacc ttttcctcca
    38701 tagactgcta ctgaggagaa attgatgatg gtgttgactc cagctttttc tgcttcagag
    38761 attagcactt ccgctccgcg aacattcgta tcaaaatagg cttcatcggc aataccaaaa
    38821 tcgtgatgca tggcagccaa atgaatgatc atatcgactc cttgaagcgc atttcgaaca
    38881 tcattcaaat tccggatatc gcctgcatga tagcgtgcat gtgaagtata atctggcgcg
    38941 cgtaaatcga gattgactat ctctcgatct ttaaaacgtt ccacaaaata ccgtccgata
    39001 aatcccgaac ctccggtaat aagcaatcta gccatcggcg cgaaggtaca ttcatttttt
    39061 ttccatctgc ttagaaggaa tatatttgcg catatgggca gtaaaaatct actttattac
    39121 cagtctagca tattactcat cttagcaata gggatacttg ttgtagttaa ttacttcaat
    39181 caaagcacct acgacacagg cgattcggtc atgcattttt tgtatgctcg ccatgcgatg
    39241 gatcatccaa tgctttacct agaacattgg ggaaaacctc tttttacctt gttcgcttct
    39301 ccatttgcca ttttcggctg gacaggaatg aaagttttta atttgctcgt aacgttattg
    39361 agtatgttct ttttgtcggc aacggcagaa aaacttctcc ccaatagtcg tggagcagct
    39421 gcattaattt ttgcatctgc cccacaactt ttattaatga cagccagcgg acttaccgaa
    39481 ccattatttg gtttgttaat gagtatcgct atttggtccg aatcaagaaa aatgtattgg
    39541 ctaggagctg taattatttc tttgagtctt ttttctcgtt ccgaaggtca aatcattctt
    39601 attatttggc tgtgttggtt tgtctatcaa aaacgttgga aagaaatccc tctatttttt
    39661 agtgcattta tcgtgtacgc aatcattgga tatttttcta aataccatga tttctggtgg
    39721 tattttaaca agaatccata tgagcaacct cgttttgttt acggttcagg gccatggaat
    39781 catttcttag tcagtttcat caatattact ggtccaattt tacttgttct gaccttcatt
    39841 ggaattattg gctttattta tatagtacta ccggcattct tgattaaaaa ggataaacaa
    39901 aaaggtgttt gggtattagc atttctgagt gttgctggat tcttcttttt ccattcgtat
    39961 gcttgggcaa gtggacgatt tggttcttat ggcttactcc gagttatttt gggaatttac
    40021 ccattaaccg ttttactcat ggtttatgca tggcaaagtt tagcggataa actaaatgga
    40081 aattggaagt taatcgtgtc caatggtgtt ttgctattgg ctttgatata tccttttatt
    40141 ggttcaaaat attcaattca taaagttgat ttgcaaccac atccctcttt aatgcaaatg
    40201 caagaatgtt tggctgatat gaatcaactt acttatcctg ctctagtttc agcagcgccg
    40261 cttcctgcat ttgttttagg ggttgattat tttgatgata ccaaattcag gtacctcaat
    40321 acagttgaat tgccttatac tcaggaaggt tcaataatcg tttgggatca tcattttgcc
    40381 ggttctgatt tgaaactacc tgcaaatgct tttttgcaag acactgctcg ttttgaactc
    40441 atcaaaactt gtgttccaaa cgagggacaa aaaggagatt caatcctagt ttttaagctt
    40501 ttgatagctc gataatcgta atctccggtt taattccaac acggccgaga taacctaaaa
    40561 agcctaatcc acggttcaca tatatatgtt gctttccttc ggaatataat ccagcccatt
    40621 gcttatatac atattgcgat ggactccatt tgaatcccgg gatttcaatc ccaaactgga
    40681 atccatgcgt atgtcccgat aaggttaaat caatatcgga atgacttttt aatacttgcg
    40741 catgccaatg tgatggatca tgtgaaagca agatttttac aggaacttcg ggcattccag
    40801 cttttgcttt tacgatatct ccttttcgtg ggaaacgtaa agcctcgccc caattttcta
    40861 ctcctaacaa cccaatctga tcaccatttc gctctagaat ccggtgttcg ttgttgagca
    40921 tatcccagcc catttctttg tgaatttcct ttagacgttg caggttggct gcttttgctt
    40981 ctctcgaatc ccattccaca taatcgccat aatcgtgatt acctaaaatc gaataaacac
    41041 ctaaaggagc acttagcttt ttccaagttt caatgtgtcg ttcagcctca gccgatttat
    41101 cattgactaa atcacccgtg aagaaaatca catcaggttt ctcactcatg atcatctcaa
    41161 ctgcccgttt gaaaggctca tcactcgcaa aagaaccact gtggatgtct gaaatctgaa
    41221 caatcttcaa tccttcaaat ccagctggta gattggcaat tggaatttta tttcggcgga
    41281 tggtaaagtc gaaggctgtt tttatcatcc cataaatcat cgaggtaaat ggaagtgcag
    41341 caaccgttaa tgctgtataa ctgagaaatt ttgctcgact aattccacca gattttgctc
    41401 catcggccgg tataactcct tgattgaaaa aaccaattac ccaacgaacc aagcgttgta
    41461 aatcatccac catcaaaaat aaactaccaa tgagcttgct gattactacg atgaaaatga
    41521 agcagaatac atacaaatag atgtacttat tgaaccaagg attttggtaa gtaagcatcg
    41581 aaaagagacc aaacaacaag ctaaatgcac taaagcccca gtaaatacga aaagtccaca
    41641 ttctgcgaac atctgtccat ccgcttgata tggtttttac tgcctggaaa aaataaatgt
    41701 cgattagcag taatactgca aagaggatga taaaacgaat aagcatgaaa gtagccaaag
    41761 gttcagaatg tgggcgcgtt tgtcaaatgg catgcaattc gggcaataca agcatgcaag
    41821 tacccgaaca atatactggt gaaaaggttt tcgccgcaaa catacaggat tccaagctat
    41881 ctgttgccaa ggacacgagt ctggaagacg agcgacggaa agtcgtattt agtgccattt
    41941 ttgccggatc actcatttta gtgctctggg tggtgaaaat ctacgaatgg attttcgatc
    42001 tcgatttaca cgaattcgcc cttcgcccaa gaacattgtc tggaattcca ggaatctttg
    42061 cagaaccgct actacatggc gattggagcc acctaatttc gaacagtgtt cccttatttc
    42121 tgcttttgat ggctactttg tatttttata agggtatcgg tttcaaagtt ttaggttata
    42181 tctggattat caccggagtt ggagtttggc tcattgcccg caacagttac cacattggtg
    42241 caagtggaat cgtctatggc ttagcttcct tccttgcttt aagtggggtt ctccgtaacg
    42301 atactcgatt aatgtcggtc tcgctgctaa tcatctttct atatggtgga atggtttggg
    42361 gcgttttacc tttatttcaa catatttcct gggaatcaca tctcatgggc gcactcgccg
    42421 gaatattttg tgcaattcgc taccggaaac aaggtccacc tattcgtaaa tattttgagg
    42481 atgaagaaga aattccgctc agtccttccg aagaaatggg gccttttctc ccgccagatt
    42541 tccatccggg gcaaccttct caaattcatc ccggaggttt ctattaccga tatgtaccga
    42601 aacagccaga agaggaagca aaaccctaac ttcgcggcgt gaattattta caagtagatc
    42661 gtctctccaa atcgttcgga gaaaaagtat tgttcgaaaa catcaatttc ggacttcaac
    42721 aaggccagaa aacagcactc gtagctaaga acggagctgg aaaatcaact ctcttgcgca
    42781 tcctgatggg aaaagaaatt cctgatacgg gtgaagtagt ttttcggaac gactgtgaag
    42841 ttgcatatct agatcaacat ccgccatttg agccggaaca aactattgtt gaagctgtat
    42901 ttgcttggaa agatccgcgt ttggaagcca ttcgagaata tgaggaatta ttggaaacag
    42961 atcctcacag tgaacgtctg catgaggtaa ttcaaaaggt tgacgatctc aaagcttggg
    43021 atatggaagc caaagtgaag gaaatcttag ggaaactcga tatccatcac ctcgaacaga
    43081 aaatggggca actttcgggc gggcaacgga aacgtgtgtc attagcggca ttgctcattc
    43141 gtgaacccga tttgcttttg ctcgatgagc ctaccaacca cttggatgtg gaaatgatcg
    43201 aatggctcga aggttacctg cgacaaagca atcttacttt gttgatggtt acccacgacc
    43261 ggtattttct agataatgtg tgtaccgata ttttggagat tgatcgtaag tcggtattgc
    43321 gttacaaagg aaattacgcg tattacttag agaaaaaagc ggctgctatt gagatgttca
    43381 acagcgaagt ggagaaggca aaaaacacat tcaccaagga attagagtgg atgcgaagaa
    43441 tgccgaaagc tcgcggtacc aaagcaaaat cgcgtatcga cagcttttat gaagtaaaag
    43501 aaaaagcttc tcaaagacgt aatgacgata aggttgaatt aagtgtgaag atgaatcgtt
    43561 tgggaggaaa aattctcgaa ctcatcaagg tttcaaaagg atatgaagga cgtaccttga
    43621 ttcagccttt tacttatacg tttatcaagg gagagaaaat tgggattgta ggcaagaacg
    43681 gttgtggaaa atcgacctta ttaaagttga ttatgagcca actggaacca gattccggta
    43741 aaattgtggt aggcgaaacg gtcatcttcg gttactattc gcaggatgga atgaagctag
    43801 ccgaagataa gcgggtgatt gaagtagtaa aggacattgg tgaatatata ccgttggcta
    43861 acggaacaaa cctcacggca tcacagcttt tgcaattatt tctttttccg cctgagattc
    43921 aatacaccta tgtttctaag ctgagcggtg gagaaaaacg gcgtctatat ttgctcacgg
    43981 tcttgatgaa gaatccgaac ttcctgattc tcgatgagcc aacgaacgac ctcgatattc
    44041 aaacactcaa tattcttcag gatttcctag agaatttcag tggttgtgta ttgattgtat
    44101 cgcatgaccg tttcttcatg gataatctag ccgaccatct ttttgtattt gaaggtggcg
    44161 gagtgattcg cgattataac ggtaactaca ccgattatcg tcaggagcag gctgttttag
    44221 ataaagcgag taaatcggaa ccggcagttg ttgttcctga agtagttgta aagcaagccg
    44281 cacctaagaa agagaaatcg aaaccgagtt ttaaagaagt tcacgagcat caacaactcg
    44341 aaaaggaaat tccacagctt gaagaacgtt tggcaaactt aacaaccaag cttaacagcg
    44401 gaattaccga tcatgtggaa cttcagaaaa tagctcagga aataacagat ttatctaacc
    44461 aaatcgagga aaagtcgatg cgatggttag agctttccga aattgtaggt taagccttat
    44521 ttatcaccaa ggttttggtc atttggtcat gtaatccttg tcttttcaag gtgaacaaag
    44581 ccatcacgca accaaatcca gctgtaaaaa aggaaaacaa tcgggcgaag tagcggaaga
    44641 aagcttgtgg aacagaaagt tgattgtgat caactgagca gattttgatt ttgagagcta
    44701 atttccctac agttgcgcct aatcgactac tttccatgag tatcgaatac acaccatata
    44761 cttgaaagaa aaataaagtg ttgatttttc gaaaggcttc atcaccagcg aattctcctg
    44821 tgattatcca agaaattaat cctgtaatta tacttacaag aatccaatcg ataaaagtgg
    44881 caactactcg tttccaaaag gctgcgtact gttctaccat agcttgagtt gaactacaaa
    44941 ttaatgacaa atcgaacggt tcttttctat ccttttcact attctatcag aatcctatct
    45001 tcaccaaaaa aatacgcatg aaaaaattgt tgcccattgt actgtttgca ctgtttttaa
    45061 gtgcttgcca atcgaccgtt tcgtacactg gattgcgacc tgcagatgtg aatcttccaa
    45121 ctgaaataaa atcagttgta ttagtgaatc gctacaaagc cgataaaaag aactcttgga
    45181 taaatattgt tgaaggtatt tttacgggtg aagtcatttt tgctgataaa cgtggtgttg
    45241 aacaagcgtt atcaggcctg caacaacgtc ttcagagtgg tccaaaatat acggttacaa
    45301 ttgctaatga tcaactaact ggaaccggca caggtgtttt gcctccacca ttgagtcaag
    45361 gtgatattca agcgttatgt acgcaataca atgctgacgc ggttattaca ctagaagctt
    45421 tcgattcgga tattgcagtt actcatcagc aaaagattaa taaacgcacc gaaaatggga
    45481 aagtgattga agatatttat tttgaagctt ttgaaaatgt gcgaatttct atgtcttggc
    45541 gtttgtacct taaaagtaca ggatccattt tcgatcagca tcaaatgagc accaatcgtt
    45601 cattcagcag tacaggtaaa actcctgatt tagctcgagc tgctttatta tttcctgttg
    45661 atgccattat gcaaacagga tttcagggtg gagattcgta tggaattcgt atcgctccat
    45721 cgtgggtgag ttataatcga atgatatata cccgtgcagc acgtcgaggt ggaatgaaga
    45781 tcgctaaacg catggcttat cgctccgagt gggctgaagc tgctacaata tgggagaaat
    45841 tgagccagtc gtcgaatcga aaagtagcta aacgtgcaat gtataatcgt gcagttgctg
    45901 ccgaaatgtt ggggaatttt gatgaagcat tactatgggc tcgtaaagca gctaaccaac
    45961 atggtcttcg cgcagccgat aattacatct atcagctcaa cagccgattg aacgaattac
    46021 aacgtttgga tcaacaaatg aatccgaatc cctgaagtac atgggtaaac cagtagaacc
    46081 ggggtattca gtcgaacagg aattaagcga aattcaagct tcgctgaaaa atccacggga
    46141 attcgagaaa atctattctc gtcatttttc tacaatttat gcctacatca aaagtgccac
    46201 tggagataaa aatctagcag ctgacttatg ttcggatgta ttttattcag cattaatcaa
    46261 tcttgctaaa tacactacca gtgaaatggg aatcagacct tggttatttg gtattgccat
    46321 aaatcgtctg cggatgttct ttcgtcaatc gggacgaatg atgtatgtgc caattgatga
    46381 tttaaaattg caacaactaa cagaggagga cttgtcgctc gagaagaaag aaaggaggaa
    46441 gcaattatca gcctatttat ctttacttaa cgaagaagag caagagtttg ttcaacttag
    46501 atttgtagcc gaatgctcct ttcgcgagat tgcacttgtc aaaggaatta gtgaggaagc
    46561 ctgtaaaatg aggctttatc gcttattgga aagaatgcga aaatcaatgg ataaggatac
    46621 cttattatga aagagttcaa tcgtaaaatt caggtaaccg attccagtca gcctgatgaa
    46681 agagagcttc aggaagcacg tgatttttct cgcattgtca cccgattgga tgaagcgcgt
    46741 aaaccactac cgaaacgtaa attatatcag tcgaaacaac gttggttttt tctcgctata
    46801 cttttaatca tactgatact gttgatttta ctcgaagtat tctaaaaacc gttcttctta
    46861 ccgttacttt ttttctgcgc gtgcatttag ctaataaatc ctcgctatga aaaaatcacc
    46921 taattcagtt ttgctagcat gtattcttta ttgcatgccg ttttttgtat gcgcacaacc
    46981 tgatatcaaa gattcccggt atttaaaatc gggttttgtt cattgcacca taacttccat
    47041 gaaaggcatc cctaagcaag gcgagaccgt tgtatttgag ggagaaaaat ccaaaaagca
    47101 agttcaatcg gtttccgact caaagggaat ttgtgatgta tggctaccca aaggggatgt
    47161 gtatttaata aaagtgctaa ctgttgggaa ggaagaagat tattcgagca ttgaagtgcc
    47221 cgataaacct ggagcgtttc gaggaaacta ttccatacag tttgatttac ccacaacagt
    47281 tacactcgat gacgtgcttt ttgaaacagg aaaatcggtg cttaaacctt catcttttcc
    47341 atccttgctc aaagtagccg aattactcaa gcataagaaa ggactcgtga tcgaagtggt
    47401 tggacatacc gataatcgtg gtgatgctgc tgccaatatg aaactgtcgc ttcaacgagc
    47461 tcaagctgta aaagcatact tagccgaaca ttcgggtgct gcagagcgga ttaaagcaga
    47521 tgggcgtgga gccgatgagc caattgctga taatgattcg gaagaaggac gtaagctaaa
    47581 tcgccgaacc gaaattcatg tattgaaaga gtaatcagat tcctatcgga tgccaggtcg
    47641 ttttcacttc caaataatcc ataattcggt aaggtgattc ctgcttggca tctgaccaat
    47701 ttggctctgc ttcgatacga actcgtttca cattcaaagc agcattcgtt tcggccgatt
    47761 ttttctgtgc atcatcggag gtgcagatgt acaaggcatt cacatccatg tgcgtggaga
    47821 agtgcgacaa taattctttc gtttccccag taagtatgtt gactactccg cccggaacat
    47881 cagaagaatt aagcacttca gcaaatgtta ctgaacacaa aggaaggtcg cgggaagcta
    47941 aaacaacaca ggtatttcct ccagcgataa caggagcaat aacagagatt aacccaagca
    48001 atccactttt ttgaggtgca atcatagcaa caacgcccat tgattcggtg gatgagaagt
    48061 tgaagtgtga actactcacc ggatttactg tactgaaaac ctgattatac ttatcgcacc
    48121 agccggcata ataaatcaag cgatcaactg ataattccac ttctgctttt gccgaagctt
    48181 ttgtgcttcc ttgttggatt aattcttcaa caaattgagc cgaacgcgtt tctaacattt
    48241 ctgctatacg atacagaatc tgactgcgat ttaaagctgc tctacctgcc caactatctt
    48301 gtgcgtcgcg tgctttcaca acggcatttc taaaatcctt gcgggaagaa agacagatgt
    48361 tgactacttc accatcgctt aaagtcaatg gataatagcg acccgattct gttctaggaa
    48421 attgtccgcc tatataaatt ttataggttt tcaaaacctt gattcggttg cttgatgttg
    48481 aattggaatc agctttacca ttggcactca cagcagattt agtttctgct ggaactgctt
    48541 tggttacttt attcggttta gctgttgctt ttgttgatgt tgccatgaga aaattctttg
    48601 aaatcaggaa aattggagat agggtagaag tccgtgaagg cctccttcgc gaccaaagcc
    48661 actttccttg tatccgccaa atggtgatga cggatcaaat ttgttatagg tatttgccca
    48721 aacaacaccc gctttgagct tggttgtcat attgaaaatc ttggaacctt tatctgtcca
    48781 tactccagca ctcaatccaa atggtgtatt gttcgctttc tcaatcactt cttctactgt
    48841 tctaaacgtt tgaacagcta gtacaggtcc gaaaatttct tcctgaacta cgcgatgact
    48901 ttgagatgta tgtaaaaaca aggttggctt gcagaaatat cctttagctg gaacggaaca
    48961 attcggctga taaatctcag caccttcatt cacaccaatt ttcaggtatt cgttgatttt
    49021 cttgagttgt tctttggagt taattgctcc gatatcggtg tttttatcca tcggatcgcc
    49081 tacaattaag ctttccatcc gatctttcaa tttgcgaatt accttatctg ccacgccttc
    49141 ttgaacaaac aaacgagaac cagcacagca aacatgacct tgattgaaga aaatcccatt
    49201 tacgattcct tcaaccgctt gatctaaagg agcatcttca aaaatgatat tggcagcttt
    49261 tcctcccaac tccaacgtga gtttcttccc agtacctgca attgctttct gaatgatttt
    49321 acccacatcg gtcgaaccgg taaaagcaat tttgtctaca cctggatgat ttacaatagc
    49381 agctccggtt gcaccagcgc ctgtaacaat attcactact ccagctggta atcctgactc
    49441 ttgaatgatc tcagctaatt tcaaagctgt tagcggagta gttcagctgg ttttagaacc
    49501 acggtgtttc ctgttgctaa tgctggagca attttccatg ctgccattaa taaagggaaa
    49561 ttccatggga tgatttgtcc ggctacgcca atcgatttta cttctctacc cggaaatgca
    49621 tattctaatt tatctgccca accagcataa tagaagaagt gattagctgc taaaggaaca
    49681 tctatgtcgc ggctttcacg aattgatttt ccaccatcca tggtttcgat cactgcaaat
    49741 tcacgagcgc gttcttgaat caaacgagca atgcgataaa tatactttcc acgttccttt
    49801 gcgggcattt tgctccacac attttcgtat gcgtttcgag cagctttaac agccttatcg
    49861 acgtcttctt gagttgcttc tgctacacga gcaagcgact gttcggtgga tgggctgaat
    49921 gtttcaaaat agccaccttt aacaggtttc acccattcac cgttgatgaa aagctcgtac
    49981 tgcttttgca aacgaatgtg tccggttgat tccggagccg gagcataagc aagcttagaa
    50041 atgaaatcga gcgacaattc tgacgcgtcg gctgatttcg atgatttatg cgattttttc
    50101 gacatggtat gattggattg gggcaatgcc cttagtcgtt cgagaaatag ttcagacttt
    50161 gataaacacc tgtttcctgt ttaaccagtt gcatgatgat atcgtttgtc aatttagagg
    50221 ctccaaagcg gaaccaatca ggatgaagcc aatcagcacc aagggtttct ttcaccataa
    50281 ctagataatg cagtgcgagt ttagaattgg caattccacc agcaggtttc attcctatca
    50341 ttttacccgt tgcgtagtag taatctcgta tagcttcaag cataaccaag gtaacaggca
    50401 tggtagcagc aggttgaatt tttcctgttg aggttttgat aaaatctgcg ccagctaata
    50461 tggctaagtc ggaagcttta cgaacgttat caagtgtgct gagctctcct gtttcgagga
    50521 taactttcaa gtgagctttt ccgcaagctt ctttgattgc agcaatttca tcaaatacga
    50581 aattgaattc accggaaaga aatttacctc ggcttatcac catatcaatc tcatcggcac
    50641 cttcttcaac cgcaaaacgg gtatcgtcca acttaacttt tagtggcgct aaaccactag
    50701 gaaatgctgt agcaacagag gcaacatgaa ttttacttcc tgccaaagct ttacgggcaa
    50761 ctttcaccat ggttggataa acacaaactg ccgccacctg aggaattccc ggaaaagcat
    50821 catgtggatg catagccttg taacacattt gctgaacttt cccaaccgaa tctttccctt
    50881 cgagggtagt taggtcaatc atgctcatgg cgagttttaa gccggcaatc ttagtttcgt
    50941 cttttatgct tcgtgtttgg aagcgtgcac atcgttcttc aacgccaacc tgatctatgg
    51001 tcggactgag tgcaggattg tatgatttta tagttgttcc catctgcggt tcttgctcaa
    51061 aagtaggaaa tttgaatcaa cgtaacttac gtggtaataa tggcaaacaa atccaagcta
    51121 acaaacaccc taaagtattt gcagcaaagt cgccaaaatc ggctgttcta tcggtataaa
    51181 atgcccattg aagaaattca attaagccac catacatcac cacaaaaacg gtaacccaaa
    51241 gacttgaaat tcctgaattt gttccaaagc gttgccagcg taaatacaag agattcaata
    51301 atccaaacac acctaaatgg gcgaatttgt caaaattgat cacatgtatt tccggcaagt
    51361 ctttaccggg caataaggtt agcgtactaa caacgattgt ccagattatg gcaggcagaa
    51421 tatatctgcg catggaatta atgtccgatc aatgccttgt aatcggcagc tgaaagaagt
    51481 ccgctagtat caccaggaga gctcattttc actttgatca tccagccatc accataagga
    51541 tcgctgttta ccgtttctgg tgaaccatcc aagatgttgt taaactcaat gatttcgcca
    51601 gcaactggca tatataaatc ggatacagtt ttaaccgctt ctactgaacc aaaaacttgt
    51661 tctgcttcaa ttgtttcacc aactgtatca acttctacga aaacgatatc acctaattcg
    51721 ctttgagcaa attctgtaat gccgattata gcagtgtcac cttcgatgcg aacccattcg
    51781 tgatctttag tataaagaag attatcagga aaattcatgc gtgttgattt tgtttccaaa
    51841 cctacgtgtt ttgttcacat acagcaagga aaccgagtaa ataaaataag agattattga
    51901 tttaaagtaa agcgcaaact cagaccaaaa ctggtgtttg aatttggaaa ctgattcgaa
    51961 atgaatggct tatttgaagt acgatcgaag aataagcgaa ttgtaaactt cgaattcacc
    52021 acgtaatcgg ctgtagtttt aatcgtgaaa ttctgctgcc cggctgatag aattggatca
    52081 aacaaggttt caccttcaat tggaatttta cgtaccactg ttttatttgt acgaatagtc
    52141 acatcgcaac gtacattcaa atcgctctta atcttcttcg tagatccaac cactttaaat
    52201 ggtaactcta cattattgaa gatataaccc gaaccaatcg taatttcctt accgtttacc
    52261 tctgtgatct gagagttttg taagctcagt gataaatcgc gtgtcttacg catctcaaac
    52321 ttggtaatca agctattttt ccaagtcata tccacattca ccatcggtcc aaattgttcc
    52381 gataaagaag ccatcagaat gttgagttga ggaatgatgt tccctgtttg atcggttgaa
    52441 ttgggatctt gtattgcgag caaattagtc gtaaacgaac cgatattgaa tgttgatcgg
    52501 taagcatgac tcagggaaat tgtcttgaaa tatttcttga agaactcaag tttagtcaaa
    52561 ccatcatatg tgattcgcca gtttggtttc ggaatattcg ggaagagaga aagatcttga
    52621 cgggttggat cattacccat gtatgtcgac ataaacgaat acaacaatac atcctgttgc
    52681 gtactaccaa aacctgtcgg ataattggtt gttggatcta taccattggt tgttaatcta
    52741 cttgctactg ctttacgatt atctaagaag cgctggaaat tactcgatcc gtgaagctta
    52801 ttatccaatt tttcaaatgc agttccccaa gtatttgtcg acatactgaa tgttccagtt
    52861 ttgagtgtac tcaattcttg gaannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    52921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    52981 nnnagcatca ttaggaactt agctgcattt tcaagaatct tcgagaaggc agtttctttc
    53041 ttctctgtgg agtctttttt agcttttgga tctttcggat ctaaaggttt tacctttttc
    53101 ttttctttag gtttagtacc ttttttctga ttaatctttt tcagataagg cactttatta
    53161 tacaactgca tcagattggc ctgagcactt gaattgatgc tattcgaatt ggtgattgaa
    53221 ttcccaaaag ggttagctgc aaacttgttc aaattagaac tgtctcgaac taatggtgct
    53281 gctacccagg aataagttgc agcataagat acattaccag aaacccaatt aagaatagga
    53341 actttggaga aaggaatggt ataggatgca ttaagcgaat gctgataatt ggtatttcgt
    53401 ccaccagatt ttaggtttct tactaccgaa tctcgcttag atgatgtatt tatcaaacct
    53461 tgttcttcat caatcctact ttgattcgtc gccatgaaat cgagttgtag cgacttggtt
    53521 agaggatatt taatatcata aatccgattc caagtaaagg atttaaagaa tgttgtatca
    53581 attttaagca gagcgatatc agaggtatta cgcagttttt gtgaattgaa atcacgtgcg
    53641 atatctgaac ggaaagctaa tcgcgaaggc gttaatccaa tattgaaatc cttaattaga
    53701 gcaaaagctt tcgttttctc aaaaatctta gcttttttaa atggttccca agcttttgct
    53761 tgaggtgcgt atgtgtagcc tagacctaca tcaatgtttt tttggtagtt aaattctacc
    53821 tgaggagaac gttggaaatt atcagagaaa gcataggtaa aatctaaatt ctcaacatcg
    53881 tagaaatgcg atttagttgc tccttgtgcc ttattctttt tcacattggt gaaattgaca
    53941 ctctttcttt tctgataaac ttgcgataat tgtcttaatg tatctcgagc tccatctgaa
    54001 ggctgataat ctaatgcacg ttcgaatttt acatccgcat cgagtggatc aaattgtgga
    54061 ttagcaatgt tttcgccaaa ttcccagtat aaggggatag ataaattcca ttttttaggg
    54121 aagaatttac ctaattccaa attactaatc aaactgtatg aagttgtatt agcacgagca
    54181 cgatcagcag gttttttctc gatgcttccg aaaccgaatg tagtacgact accagcaagt
    54241 gcaatggtac ctaaatcagc aagcttagct gtaacacgag cattggcagc ccatccacca
    54301 gattcaaaat agtcggatac gcgcaactca ttcacccaaa cttcagcaca tccaaccgaa
    54361 ccatcatccg gccatggatt atattcgtcg ttttgtggat tacgaatacc aatcatcatg
    54421 gtcttaactc ctgaaatctg cggaacacct acaacagtaa ttcggttttc tggttgattt
    54481 ggatcctgag catagaaagg tttgctgtat gtgattaatg gattattcaa catttgagta
    54541 ttccgatcaa tcttcgcgtt ggtaagcgtt tcgagcaaga aattgaaact gttattccag
    54601 atgtcttctg gattagttgt accatcagca catcgtttca atgggatttc atattcgtaa
    54661 tagttattta cgaaatcggt tcccaatcga ataaaagctg ttaattgttt atcaggccat
    54721 tgattattgt tcatcgactc agcgtgaact tccatgcgaa tgcgacgata agcacgaata
    54781 tccaaattcg tgtttttaaa tacagcacgc gcatcgccat cttccaagtt acaaattcgc
    54841 atttgtaacg actgctcgtt taattgttga agattggttg tggttacatc tacttctcga
    54901 cggatttgag gaggtaacac atatttaaca ggtgttttat ctccattctc ttcaatattt
    54961 acagtagaaa cctgaaattg agtattcaaa tcgttaccaa taaataaccc cggttctttt
    55021 aagctgtaat caaaccttct ccattcagca cgaactaatt ctaaacgagc catccgaact
    55081 acaacttgtt cttgccaatt atggaagaac atccgcataa aacgtattga acggaaatca
    55141 cttatagttc ctactttgtt tgaataattg cgaatcggaa cacggaattg ataccatata
    55201 acatcaatcg gtttcccgtt gggtaattca ccttttcctg gtactttatc gatgatgtaa
    55261 ttttgtccaa ctaccatgtc ttggggacga agactcaaac gatattggaa atacgcttca
    55321 gaggtgctta aagtattatc actattgata tcctcaatat taggaatggt attattggca
    55381 gtaggatacg tttcaattgg atcttctcct aaacgagagt tgttttctaa accgttatat
    55441 aaggtgtaac gggttaaaat atctgccgat tgagcatccc attctgtact tctgaagaat
    55501 cgataatcat catttgatgg gtcagattgc aattgtgtat atgcatctgg attcaatacc
    55561 gattgcgctt cacttaaata atcttggaag aatacacgtt ccgaatcatt cggtaatcca
    55621 tctaaaccta catcttgtag tgg
//
LOCUS       DAOOQW010000002        44991 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_917_length_44991_cov_6.031200, whole genome shotgun sequence.
ACCESSION   DAOOQW010000002 DAOOQW010000000
VERSION     DAOOQW010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 44991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 44991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44991
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_917_length_44991_cov_6.031200"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="PK281_00250"
     CDS             complement(<1..569)
                     /locus_tag="PK281_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013684976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HOY47793.1"
                     /translation="MEFLTSLLDFILHINSHLDQLVLDYGSAIYAILFLIIFVETGLV
                     VMPFLPGDSLLFAAGAICARPGSELNVIVLIALLIFAAVLGDNVNYTIGRFAGSKVVG
                     WKFRGKNIVKQAYIDQTHSFFEKYGTKAIIMARFVPIVRTFTPFVAGVGQMNYKKKFL
                     PFDLAGGFLWITTMTLAGFFLGNLEFFQK"
     gene            complement(587..1171)
                     /gene="rdgB"
                     /locus_tag="PK281_00255"
     CDS             complement(587..1171)
                     /gene="rdgB"
                     /locus_tag="PK281_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004571074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HOY47794.1"
                     /translation="MELVFASNNAHKLTEIQAILPSQIILKSLSDIGFTQELPETTGT
                     ISGNALQKAKTLFQLTGHPCFADDTGLLVKALDDKPGVDTAHYAGIERNALANMQKVL
                     KELKGHSNREARFITVIAYVDGNREHVFEGEVKGRIAEQITGDGGFGYDPIFIPEGFE
                     LSFAELPVTTKNSISHRARAMALFVDWLKDLPVN"
     gene            1233..2330
                     /gene="gmd"
                     /locus_tag="PK281_00260"
     CDS             1233..2330
                     /gene="gmd"
                     /locus_tag="PK281_00260"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013549933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOY47795.1"
                     /translation="MKTALITGITGQDGAYLAEFLLHKGYTVHGIKRRSSLFNTDRID
                     HLYEDPHINNAKFFLHYGDLTDSSNLIRIIQEVQPDEIYNLGAMSHVKVSFDEPEYTA
                     NVDGLGTLRILEAVRLLNLTQKTRIYQASTSELYGLVQQVPQSEKTPFYPRSPYAVAK
                     LYGYWITVNYREAYNMFAVNGILFNHESPNRGETFVTRKVTRGVAQIALGMQDKLYMG
                     NIDAKRDWGHAKDYIEAMWRMLQVDEPEDFVIATGETRTVREFLIRAFAEAGIQIEFR
                     GQGVDEQAFVVSCSHPDYQLPIGKEVVSIDPTYFRPTEVELLIGDATKAQQKLGWTPK
                     ISFDELVKDMVASDLQLFRNQKYINEYNSGN"
     gene            2338..3264
                     /locus_tag="PK281_00265"
     CDS             2338..3264
                     /locus_tag="PK281_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011708476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HOY47796.1"
                     /translation="MEKSDKIYIAGHRGMVGSAIWRALENKGYTNLIGASSKEVDLRR
                     QSEVEAFFEREKPDVVVLAAAKVGGILANNIYRAEFLYDNLAIQNHVIHSSWKNDVKK
                     LLFLGSSCIYPKLAEQPLKEESLLSGFLEQTNEPYAIAKIAGIKMCESFRRQYGCNYI
                     AAMPTNLYGPNDNYDLKNSHVLPALIRKMHTAKVNQDAQVEIWGTGSPLREFLHVDDL
                     ADACVFLLENYSEELFVNVGYGSDISIKDLAYLVKDIVGFAGELRFDPEKPDGTPKKL
                     MDSGRLRSFGWVPKISLEEGIKRTYEEVKQQF"
     gene            3336..4193
                     /locus_tag="PK281_00270"
     CDS             3336..4193
                     /locus_tag="PK281_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012781970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOY47797.1"
                     /translation="MSQKSTHEWNMIIRPSGGLFDLRLKELWEYRDLLYLLVRRDIIT
                     VYKQTLLGAFWFVLPPILNTVIYFVLFGVIAKVSTDGTNPILFYLTGLIAWGYFSDCI
                     SRTASTFRTNASIFGKVYFPRLIVPLSSVVSALVKFGVQLILLIGTLVFFLIRGENVH
                     PQMTYVWLLPILLLMMMFTGLAFGIIISSLTTKYRDLSNMVPFAVQLLMYATPVIYPA
                     NVIPEKFQWILQINPLTPIIESIRYVFLGSDKISWPMLGYSFAFTLVTLFIGLILFNK
                     TEKNFMDTV"
     gene            4225..5508
                     /locus_tag="PK281_00275"
     CDS             4225..5508
                     /locus_tag="PK281_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007648647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOY47798.1"
                     /translation="MSVVVQADQVSKVFRLGDVGSSTITEDIRATWYKLRGKENPYLN
                     QASENDRTGGVGSDYVWALKDINFEVNQGEIVGLVGRNGAGKSTLLKILSRTTTPSHG
                     EIRIRGKVASLLEVGTGFHPDLTGRENIYLNGAILGMRRAEIRAKFNDIVEFAGVQRY
                     IDTPVKRYSSGMYVRLAFAVAAHLEPDILIIDEVLAVGDAEFQNKCLGKMKDVSDQGR
                     TVLFVSHNMGAVADLCSRAILLNQGKIVQDGDTNEIIRSYVHQNMHVKLSDASALSDP
                     TVRRGLGHVRFEQIRMLNRAGEEVNEFQKGDYMCIEMTARIINPVKELLASILLRSGK
                     TRDIVTSTRRNTVDISHLKPGDTVNLRYEFPSLSLRPGVYETYYWLGDKNAETAFDVV
                     DNLLPPLVIQMPPDSSELFVSGYFDEPFIFEQFTR"
     gene            5525..6535
                     /gene="pseB"
                     /locus_tag="PK281_00280"
     CDS             5525..6535
                     /gene="pseB"
                     /locus_tag="PK281_00280"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase
                     (inverting)"
                     /protein_id="HOY47799.1"
                     /translation="MPLNEKRILITGGTGSLGKKLVEIILKKYPDIKRLVILSRDELK
                     QFEMAQDYPESKYPCIRYFLGDIRDASRLQRAFEGIDIVIHAAALKQVPAAEYNPFEF
                     IKTNVLGAQNIIDAALDCGVKAVVALSTDKAAAPINLYGATKLCSDKLFVAANNIKGK
                     HDVRFSVVRYGNVMGSRGSVIPFFLKKKGEGTLPVTDPDMTRFNISLEEGVDMVLYAV
                     EHAFGGEIFVPKIPSYRITDVVKAIDPTAKIEVVGIRPGEKLHEEMITSSDSYNTVDL
                     GKYYAILPQANNRIQQFYMDQGGKMVEPGFCYNSGTNDSWLNETQLRELIDQFVSVTE
                     KA"
     gene            6557..7099
                     /locus_tag="PK281_00285"
     CDS             6557..7099
                     /locus_tag="PK281_00285"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HOY47800.1"
                     /translation="MSFSEIETERLILKPMDESFISEEYLSWMNDPEVYLYLETGGNY
                     SMDELQEYVKQSVKKKTLFWAITIKDNGKHIGNIKIDPISQRHGTGEYGILLGDRASW
                     GKGYASEATSGLLNYCFETLGIRKINLGVVKDNVSAVRLYEKLGFKTEGEFEAHGFYN
                     GKYCNTLRMAVFNPAFTHEK"
     gene            7089..7949
                     /locus_tag="PK281_00290"
     CDS             7089..7949
                     /locus_tag="PK281_00290"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOY47801.1"
                     /translation="MRSKLALGTVQFGIPYGINNTTGIPDQDELNSIFSTAQLNGIQT
                     FDTAIAYGNAEERIRPFLFEGAQVISKFPPASQAHTLAQELDESLKRLGQNHLYAFMA
                     HDADLLLKQPDLWAELIRLQADKLIIKKGYSLYHPEQLEKLLEADFIPEIVQVPYNLL
                     DRRFGPAMKQLHDMGVEIHIRSVFLQGLYLMELSKLPEKLQTISSELKQLHDLALSEN
                     VSMSALALNFAIQNPHIDQVVIGVDSSEQLRMNLKVLNSWDDKKNTIFSQVMQLTCSH
                     PELLIPSNWK"
     gene            7946..10042
                     /locus_tag="PK281_00295"
     CDS             7946..10042
                     /locus_tag="PK281_00295"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: protein motif:HMM:NF004856.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HOY47802.1"
                     /translation="MKVIGITQARMGSSRLPGKVMLEAGGKTLLEHHLSRAKQSKKVD
                     SWILATTQEPGSEAIAAIGNKLNIPVYHGSSDNVLDRFYQSVKDLRPDYVVRITSDCP
                     LIDPKLIDLAVETTIQNKLTYYSNSNGDTLPDGMDAEVFTFESLENAWKHAELASDLE
                     HVTQYIRRNAEVNFRSSYPKEWKKYESLRLTVDEPVDYELILKLIEKYGDDQEWLTYA
                     DAVLANADFAAINQHIIRNEGFMKSVFQELELRSITNFTKSDEYRAEIHSIIPGGAHT
                     YSKGDDQFPIKSPAAFTHGKGAHVWDLDGNEYIDCSMGLGSVSLGHAFEPVLEKVRAE
                     LLNGVNFQRPSSLERVIAKKFLSLVPQHDMIKFAKNGSIVTTAAVKLARAKTGRKLVA
                     FPGDHPFYSYDDWFIGKTGCNKGVPEEFSNLSVTFKACNIESLRELFRQYPGQIACVI
                     TEPEKQTCVTGCACNGRPDLFLKEAIELTHAEGALFIADEMVSGFKTGFPGSFTKYGL
                     EPDMATWGKGIANGFSFCALTGKRDVMELGGITRTGEEKVFLISTTHGGETHALAAAL
                     ATIDFYEENKVVDQMHEKGRRLLELTQHIVKKHELEHAIQILPCEWTPIFLFKDASGQ
                     VSPGYRTLFMQEMISRGVHFQGFFVPCYSHTEEDLFYFAKAFEMSLPVYKKALEEGYE
                     KYLVGEPAKAVFRKIL"
     gene            10063..11250
                     /gene="pseC"
                     /locus_tag="PK281_00300"
     CDS             10063..11250
                     /gene="pseC"
                     /locus_tag="PK281_00300"
                     /EC_number="2.6.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011709895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4,
                     6-dideoxy-N-acetyl-beta-L-altrosamine transaminase"
                     /protein_id="HOY47803.1"
                     /translation="MASDQVKKQAIPYGRQTITDEDLRIVSEVLQSEYLTQGPRIAEL
                     EQSFAAYTHSKYAVAISNGTAALHLCAMALDVKEGTRVITSPITFVASANCVKYCGGE
                     VWFADIDPKTFTLDLQATRKLLESKPKGFFHGIIPVDFAGYAVDLQAFRQLADEFGCW
                     IIEDACHAPGGSFTTSTGAKAHCGSGEFADLAIFSFHPVKHIAAGEGGMITTNSLELY
                     NRLLKLRTHGITKDESLLIDNHGGWYYEMQELGYNYRLTDFQAALASSQLQRADAGLA
                     RRQEIADRYQQELAGLPIELPQVATGVTHAWHLYVIKTEKRKELYDYLRTQHIFTQVH
                     YIPVHTMPYYKQFGWKEGDFEVAEKYYSGCLSLPMFPGLTDEEQGYVIRCIREFFKSE
                     AGA"
     gene            11315..12421
                     /locus_tag="PK281_00305"
     CDS             11315..12421
                     /locus_tag="PK281_00305"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOY47804.1"
                     /translation="MMKESASMEITRIAKSYSLQGYKVFTLSIGDTHFGLPDPIKLRL
                     NKAIEENKTHYLDSMGLPALRMDISRFEFQEAYLQDEILIVPGVKQGLFYFLEAFEGK
                     KIAILEPAWLGYHSICKMTGKEIIPINMKKEGWLDKLASISFDALLVCTPNNPDGTVF
                     SESEIKQLASYCDKNQACLIIDEIYAEYSFDHSVKSMLKPYYSQENVVVMNGFSKAYA
                     ATGLRIGYVATHNPVWNKRMNAVHQNTATCANSLAQFAFLNYHESHSIVQQYASYYRE
                     NRDLVCEIIPELEEMKPQGGFYFFIDLNRFGISDAEEFSRRILTDIKIALVPGSAYGT
                     GFDSYVRLSYSLDRDELREGIQLLKKYLQDYESE"
     gene            12435..13115
                     /locus_tag="PK281_00310"
     CDS             12435..13115
                     /locus_tag="PK281_00310"
                     /inference="COORDINATES: protein motif:HMM:NF015682.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HOY47805.1"
                     /translation="MISQIERNRISSVEVADALGKTGVLNGPQILTPGKFCVGEVQYI
                     YGHSETNWPIHEQGEHLKDGGILYVDTFNCNDKAAFGDLVSKYFMLYKRIGGMVVNGL
                     LRDAHTLRKENYAIWCLGTTPLGCVNVNIPVPPDVEAEAMKRKAYFDGSILVCDDSGC
                     TMIAKELITEATLKKLEFMELQEDIWYYCLDTLKWSTYETVCQKKYLSNKSVLPTVLV
                     EKLKEYDL"
     gene            13105..13911
                     /locus_tag="PK281_00315"
     CDS             13105..13911
                     /locus_tag="PK281_00315"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOY47806.1"
                     /translation="MTYKRIPVFDTLSHPTLNGNWFGKDCGNTFENLKKEFHANDIIG
                     TCAVSLPPMTNEELQSFYTRCTSEKEIVLYPVAAFNFSEENYEDKIRFIRDTGFKAVK
                     IHIRLSGLKLDEDFDKLAEVFRLCEQFGLVVFFCTYCHASIDYTPPHPLSYYLISLLK
                     QAPKLKVILLHGGDVSLLQISQLARFNPNILIDLSYTMLKFAGSSVDQDIVFLMNNLD
                     RKICLGSDYPDYTIGQFRERVDGLLNQVIDPEKIERICSGNIQSFLEIGI"
     gene            13989..14780
                     /locus_tag="PK281_00320"
     CDS             13989..14780
                     /locus_tag="PK281_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY47807.1"
                     /translation="MQKFGIPSQLLAETDFNASDWHQAIVVFDLFDTAKTTKFLRDTS
                     NENVKICFDYFSNEEQPDLNISVLEQFHEKRSHPNYTGLDYCVLRPDFLKNPVLKEKL
                     NEVFVYIGGNGFPEVVRTIADKFSDSPFHFNLIRNQNSLDCGPLPDNFSVHFLPENLI
                     ELMNRSTFAITSPGLATMELLYLQVPSVLCPLIDFHEYFSDFLLQEDLAISRLDDFDS
                     RLLENLPRMRESARKKVDGKGVERIIHLMLNAYEEKMGSRYPLWA"
     gene            14740..15921
                     /locus_tag="PK281_00325"
     CDS             14740..15921
                     /locus_tag="PK281_00325"
                     /inference="COORDINATES: protein motif:HMM:NF014686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOY47808.1"
                     /translation="MKKKWVVVIPCGLEVIPTIEAAKKQGYSVAGVDQNPEAPAMKFC
                     DLGIVSPLNDFEHILYVLQTNKIVPECVIPIVSDKAVFPAYMLNLQFGKTPINPNILA
                     FYSKSVLRNTLNTLGFDNPFYFVVDKEGDLKHVPLKSKLIIKPDDSSGSRGITILEKP
                     NAKNLKEAYQFALGFSANKKAVIEEYIPGEEYMVDCFVHEKEVKALLVSRKEKIQDKV
                     SILIQTVNEQEFPYAKLKKLITKLSKALTYPGGPLHIEVKLQDGKFYILDLAARGGGF
                     SVYNFYVKKCLGFDFVQATLDVFLQKKLSQKCGTFREGLIYFITPEKAGTLNNLTTSF
                     QVNPKKDVLINYYFTKGQSVSLDVTDGNRLAGIYCFAKNKQALEDLFLEVKSSFHQTY
                     A"
     gene            15921..16592
                     /locus_tag="PK281_00330"
     CDS             15921..16592
                     /locus_tag="PK281_00330"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOY47809.1"
                     /translation="MNKNEIQQTIDRYNKRLQEYGETEKALGWGDKGRSRLRYHALCN
                     EFDLKNTTVLDFGCGFGDLYAYIRDNISPDFKYIGIDINPNLIEVAQKRYGSQAEFHV
                     VPYEVSEYLEQHQIKADYILSSGIFNFKLDDNIGFIESTLHVFDRFSTRGFASNFLSD
                     KVSFQAESNFHSNPGQILEMHYKYSNNVTLRNNYMPFEFSVMVNKHDAVNKDMNVYES
                     HLQYI"
     gene            16625..17665
                     /gene="pseI"
                     /locus_tag="PK281_00335"
     CDS             16625..17665
                     /gene="pseI"
                     /locus_tag="PK281_00335"
                     /EC_number="2.5.1.97"
                     /inference="COORDINATES: protein motif:HMM:TIGR03586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudaminic acid synthase"
                     /protein_id="HOY47810.1"
                     /translation="MEDIRIGKHLIGDNQPPYIIAEMSGNHNQSIERAFEIVDAAAAA
                     GAHAIKLQTYTADTITMKGVLTINDKTSLWDGYELYDLYKEAYTPWEWHKAIFDRAHE
                     KGLDAFSSPFDETAVDFLEELNVPAYKIASFENSHIPLIRKVAQTGKPVIVSTGVATI
                     AEIDECVRMLKKHGCKDIILLKCTSTYPATPENTNLNTIPHLRNLHNCVVGLSDHTMG
                     IGTSVAAVALGARVIEKHFTLRRADGGVDSAFSLEPEELASLVTETERAYLSLGRIQY
                     GVQDAEVNSVKGKRSIYVSSPIKKGEKLSEQNLRIIRPAFGLDTKYWDIVIGREAKTD
                     LEPGTPLSWDLI"
     gene            17715..19202
                     /locus_tag="PK281_00340"
     CDS             17715..19202
                     /locus_tag="PK281_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY47811.1"
                     /translation="MKVVIFNSNLLWPSHYETELELISTHLAHGDEVTQIYCDAQLPN
                     CDLNPFFVPSVCDECVHFRKQGYQAISGDFTKIRLPELSEADKTRIAQLPLEFKSLEE
                     IQALELDGFDLGYCVASSLISIIRDPKPDFKLYAPTIKSFITGSAAIFLIFKKWLIEN
                     KPDLVYTFNGRLAHTKPVLRACQQVGIECKIHERGSIKDKYSLTLNTSPHDRKYVKAK
                     IIETWNQANPEERLPIAEDFYKSKFAGTDTNWYSYTKEQIDELPENWDPNKKNVVIFN
                     SSEDEFASLGKEWKNVIYPSQADGIKQIAQELSNDSNVHFYLRVHPNLRKVDNADKKR
                     LYGLAAPNLTIIPAESPISSYKLMMMASVVLTFGSSVGIEATYWGKVSILAGKTLYKG
                     LGSTYEPESHSEVIELLRSELEPKDRTGALMFGYYYKSHGIPYKYYQPEDIANGTING
                     LRLVRTKNWKGKFKDWFFKTPALQFISERVYYKLRYERLRNYRND"
     gene            19195..20130
                     /locus_tag="PK281_00345"
     CDS             19195..20130
                     /locus_tag="PK281_00345"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOY47812.1"
                     /translation="MTELVSIVTPVYNCVAYLRETVKSVQEQSYSNWEMLLVDDGSTD
                     GSAELVDQLAREDSRIKAIHQPNAKQGKARNNGISQATGNWIAFLDADDLWPANKLEF
                     QLQKTLNTSFGLSFTDGFICLNNDMNLRAHRFGVVDTTYEGESGIRAFHEQNRIPTST
                     VIVKKELLIRTGGFPEHLSVQNCEDYLLWVKLLKEGATFISIGEALLYYRVHPTSSTS
                     QETKALFPVIEALMSMASTGTPELKNHLGEKFRQLIFRLNELGELNQAAGIIPRVIPQ
                     LYQGVQRALLLSSWKLSPAIFIRVFWRMTGGRIKN"
     gene            20369..21283
                     /locus_tag="PK281_00350"
     CDS             20369..21283
                     /locus_tag="PK281_00350"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOY47813.1"
                     /translation="MATQGLREQIVKVLSGNPITENLILKPVRNYRIRKHYEELMLLK
                     DLSPEQRQHWADRIRITKLSTDNQKINKIPTSGKLIDGKLHMHNGLVIDPLSYYGPPM
                     LRLLMENHAVHEPQEEYVFQEVLKNMKPGAVMLELGCYWGFYSMWFASKVKNAKNFLI
                     DNGDGIARAKGNFAMNKLNATFMVGYIGKDVAGSEPPVTNVDRICEQQKIDFVDILHA
                     DIQGFEMEMLETMPILTGKKGIGYIFISTHSEELHSGCIAWLKDHGYIIICDANLEHT
                     FSEDGLIVARDPSYPGIEPMAISQYQRT"
     gene            21302..22231
                     /locus_tag="PK281_00355"
     CDS             21302..22231
                     /locus_tag="PK281_00355"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOY47814.1"
                     /translation="MLISICIPTYRQLPILFNLLKTIDFQTFRDFEVIISDDSPDDEL
                     EQAILSANWTFPIRYYRNNPAKGSPQNWNHVISLAQGEWIKIMHHDDWFYDADSLKYF
                     AEAISQNPETDFFFCQSWVSNGKKNEDYIYRPNSDKLRNVSKFPADLFEANLIGAPSA
                     TMYKKTMKLQYDSKLMWLVDIEFFCRVIQAYKIQPIERGLIVTSEFQAHQLTYTLRDN
                     KATEIREYLYCYEQLCGIFNPVNRAILRRRIIHLFLQFEVKSIKEIRDAGYIGRIPGF
                     VRLYTSLMNFNAGLAQKVIGKWTYLQLKHYDYN"
     gene            22218..23108
                     /locus_tag="PK281_00360"
     CDS             22218..23108
                     /locus_tag="PK281_00360"
                     /inference="COORDINATES: protein motif:HMM:NF013681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,2-fucosyltransferase"
                     /protein_id="HOY47815.1"
                     /translation="MIITELISGLGNQLFQYAAGKSLALKKDTILKIDRTWFDLEFEK
                     QTHRTYGLDPFAIQSEIASQAEIDLYLRPQSSNILDRIQNKIKSYQPYYKQRVYLEPH
                     FEFDTNFFKTSEDAYLSGYWQSEKYFKDYASIIRQEFQFKLPLQDKNLHWQQLISSSN
                     AISLHVRRTDMVNNPDVVKTHGSCGLDYYQAAASKIAEGLEQPVFFIFSDDPEWCKAN
                     LILDWPVHYIDNNQGDFAYIDMQLMSMCQHHVTANSSFSWWGAWLNPSPSKRVISPIN
                     WFADPSKNTSDLIPATWQRI"
     gene            23167..24177
                     /locus_tag="PK281_00365"
     CDS             23167..24177
                     /locus_tag="PK281_00365"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HOY47816.1"
                     /translation="MSQPLVSVLMPVYNAGKDLAEAIQSILDQTYTNFEFIIVNDGST
                     DASRDVISNFNDSRIHLIDNEVNKGLIATLNLGIDACKGEYIVRMDADDISLPERIEH
                     QIAFLEAHSHYGLVGCWFEDFGDQIQSKVVRYSDDDTYIRIRHLYQTHIAHPTAVWRA
                     SIIRAHQLKFDPAFVHGEDYEFWVRMSAYCKMSNVKEVLVRKRDHPHNVTNKYAQIMQ
                     DTCARVKQRQFSLMGINCSTDEVNLYSRFADGEWRFSPPQMDHLETLLESIFKANKHS
                     KFIPVEAYNRYLAEKYYHLCYNNPDISKEGWKRFRKSSFSRLFKGKFQDNLKFRIKTL
                     LR"
     gene            24178..>24884
                     /locus_tag="PK281_00370"
     CDS             24178..>24884
                     /locus_tag="PK281_00370"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOY47817.1"
                     /translation="MEQEKPFFSITIPAYNRGYILPETIGSIQSQSFQNWEVIVVDDG
                     STDNTQEIIAQLSLADSRIRYVYQQNAERSAARNNGAQNALGTYLMFLDSDDKYADGH
                     LEKLAAYIESKKNPVALFFTNLSYLTENGIEIPEIPVMQLGKEFDYLLLNPITPSRVC
                     IHRDIFNDFKFDPKIVIVEDMVLWVCIATKYPVFQHTENSLIYRIHLGNSVDLSRNSY
                     YDRYKGLLRLFNDEEYQ"
     assembly_gap    24885..24894
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            25111..26052
                     /locus_tag="PK281_00375"
     CDS             25111..26052
                     /locus_tag="PK281_00375"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOY47818.1"
                     /translation="MEELNQEYRIVRASEDNIADLSRLFKESRGIHVPVDYLKRKYNT
                     AYTSKSYFAHFAYSADGTPAAFFCLFPCFVRINGEKKLAGQSADIITHVDHQRKGLFG
                     LLGRATEALAKQEGMNYLFAFPNSNSFPGFYRSLNWHHPGNFKIYSFPISGIPIYRIL
                     HKLKLGKIYSLWKSFVISMFRSKEETFIGADFESGENVCWRDESFFSYKKYNDNFVCE
                     LNGFRIWAKVEGALIVGDISRSNMPIEKLINKISRLCKLLGLDSFQIEVSEGSFYDKL
                     LRDKHAFLEGVPIVFKNLDIPEQKLSLSHTGADADVF"
     gene            26074..27054
                     /locus_tag="PK281_00380"
     CDS             26074..27054
                     /locus_tag="PK281_00380"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOY47819.1"
                     /translation="MLARIQNKLGRIGNDFIFNTGLFASNRLKETDHTILMYHGVDTV
                     GGTDFNSRHTSQADFYKHVQFLKKYCHIISLSDFFEKRFISGKPNIALTFDDGYRNNF
                     LYAKPVLEAAKVNGTFYITGLNTSNSDILWADFLNIASKLTEQVISIDGEKFEQKNGI
                     YHSLDSGKNLYSIIKDEKAGTAYKQLMTESFIDLYDFKKDLAYDDYWKLMTDLEIRNC
                     GDSKYIEVGSHGFLHNNLGTISIEDATSELQNSKEYLENIIQSKIVSVGYPDGSYTRT
                     TLDASEDLGFLYQTAAEGFRFLEDETDNRIRDRKGMYTCDTCANQLLVNL"
     gene            27339..28505
                     /locus_tag="PK281_00385"
     CDS             27339..28505
                     /locus_tag="PK281_00385"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOY47820.1"
                     /translation="MKILYVIPTMGCGGAEILLGAIARNLVKKGHEVHLVCLEPHHET
                     WPNYPDKEAMLAEVPVTIIGGSVRFRFLRKPQVDTGAFVNYVNDFKPDIIHSHLYLSE
                     LVSRSVVFSHIKYFSHGHDNMPQLRGLHFNTLWSKPLLANYWERTWLIKQYKACNNHF
                     IAISNDVKAYLESELPAFRNSVIYHPNAIDIKRFYFARTFEPVQGVFHMVSIANLVEK
                     KNHILLIDVMDVLIRKGYRVSMEVLGAGPLMEMLQQKTREKGLENHLFFRGSVGDVPE
                     RLKNAHLYVHPAWYEPFGLVLLEAMASALPVVSLDGFGNRELMKENQNGFMLPTKASA
                     EEFAAKIAYFIDHPEERQRMGLFAQEFSKHFDIDHYTDGLIEIYQNNSKATTTH"
     gene            complement(28492..29256)
                     /locus_tag="PK281_00390"
     CDS             complement(28492..29256)
                     /locus_tag="PK281_00390"
                     /inference="COORDINATES: protein motif:HMM:NF038364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AglZ/HisF2 family acetamidino modification
                     protein"
                     /protein_id="HOY47821.1"
                     /translation="MYQSRVIPVLLCNLEGSLVKTQKFKKPVYVGDPVNAVRIFNDKE
                     VDELIFLDITATAQGRRPNFKYISEIATEAFMPLCYGGGLSNLEDIRAVIKAGIEKVA
                     INSALEKDPDLITRTADVLGSSSTVAAIDVKKDIWGKYRVAVRNASKTLNIPLIEFAQ
                     MLEQKGAGELVLNSVDRDGMMEGFDLKTISEVAHAVSIPVIASGGAGNLQHLKQAIQA
                     GASAVAAGSMFVFQGKHRAVLISYPSPSELREINEL"
     gene            complement(29259..29873)
                     /gene="hisH"
                     /locus_tag="PK281_00395"
     CDS             complement(29259..29873)
                     /gene="hisH"
                     /locus_tag="PK281_00395"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HOY47822.1"
                     /translation="MIAIVDYDMGNIGSVSNMLKKIGRKSEITRDPAKIRDADHIILP
                     GVGSFDSGMEQLEKLNLVELIREKALKDKTPILGICLGAQLMLERSEEGQKAGLGLVA
                     GDVQRFKLDGDPKWRIPNMGWNQIRQVKQSPLVDVLPENPRFYFVHSYHFHLKNEADQ
                     LLLSDYGYTFCSAFEHENIAGVQFHPEKSHKYGMSLLHNFSEPR"
     gene            29952..31220
                     /locus_tag="PK281_00400"
     CDS             29952..31220
                     /locus_tag="PK281_00400"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOY47823.1"
                     /translation="MKRPYLYILVTDYPYGIGEPFLENELLENSAHFEHIYLVIPEIH
                     LIDQSIVRYSLPSNATLVPLQTRVSLTHKLKAMSQWLKQSIRKEIAYINGSYKLKFGL
                     NHFKTITGFMASGIAFSEQFQQVLRQHNHPPEKASIYSYWFTFATWGLTLIKQGNSAY
                     NIGTRIHGWDCFYYRNSANYLPLRPITISTLDWICPISETGKTHLIEKIPDVQLNKIK
                     VHYLGVDIRPSEFPICYKQGVLRIVSVSFIHHVKRIHLIIDALAKIESIQVEWTHIGS
                     WSSQTAWLQDAAREKLGGKENIQYTFTGEKTIEEVRSFLEHHQADFLICTSESEGIPV
                     SMMEALAAGTPVISTAVGGVPEIVQDQETGYLLPANPTEAEIADTLNKIVLMDELEYR
                     RLSENAKNTYLNKFQAKVNYKKFAKEILQA"
     gene            31260..32387
                     /locus_tag="PK281_00405"
     CDS             31260..32387
                     /locus_tag="PK281_00405"
                     /inference="COORDINATES: protein motif:HMM:TIGR03573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl sugar amidotransferase"
                     /protein_id="HOY47824.1"
                     /translation="MEYKICSRCIIDTPTYPDIVFDENGVCNVCHTYDQMAEKFRIEQ
                     DVLGENYLFQKIARIKEEGKNKEYDCILGISGGVDSSYLAIKLKEWGLRPLALHIDNG
                     WNTELAVSNIEKLIKQLGLDLVTYVVNWEELRDLELAYMAASVVDIDIPNEMCSFAII
                     NRKAAELGLKYIITGHNYSSEGWLPPTFSHYKYDTLNLNDIHKKFGKVKLKTYPKIGY
                     FRTLYYSKIKKIDFFSPLNYMHYDKEEAKKILIDQYNWRDYGGKHFENSYTRFYQSYI
                     LPEKFKLDKRRSHLSSLICAGQLTREEALKIIETPNYSSEELLASDRDYFIKKLRITE
                     KEFENYIHTPPKKHTDYRSYINVQAFVKKHFSFLKKFIPAP"
     gene            32384..32956
                     /locus_tag="PK281_00410"
     CDS             32384..32956
                     /locus_tag="PK281_00410"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOY47825.1"
                     /translation="MISRIIAKLIYMLQKRLKPEMIGGFPYSKSKDVRISNHTHISYP
                     KNLTVGEQVFIAHFSYIDCLQPITLGRNVYIGSYVSILTHSAHNSVRLHGKHYLNDAP
                     DLVALQTGPVTIGDYCSIGPYTIIMPGTTLGKGTMVTANSYVNPGVYPDFAILKGNPA
                     KIVGNTRDYDELLLERNPEMRSKYYLNDYD"
     gene            32949..33896
                     /locus_tag="PK281_00415"
     CDS             32949..33896
                     /locus_tag="PK281_00415"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HOY47826.1"
                     /translation="MIEILQSNQLSDTSNLPLFFTKEWLSYSIAEGGEFFYFKFSDGT
                     LIPFVTRKRYFTCIGFLIYKPYRIGKSLSADEESSCIQEMIAYIKKHKICDVILPPMH
                     VCVLQKEVPNASNYKLGLILVDINKSQEDVFALFSKTYRTQIRGCERDGFTISHSLEH
                     FDFFFRMYRINQEKNKKYPGQASHIKGILDNMPNQAKLFTVLNPEGEPEGSILLIFDR
                     EKAYYLMGAKHEEKPTHNGSQKFLQWSVMRYCQEMGCAQYNLGGFRYNLDEQDKFSKI
                     QDFKLKFGALIEEGFHFSLKISWKYSLYEKLIALKQKIK"
     gene            33893..34939
                     /locus_tag="PK281_00420"
     CDS             33893..34939
                     /locus_tag="PK281_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY47827.1"
                     /translation="MNPSLLITFDYEVFLGSKSGTIQNCLIDPTNRVLTILAKHNFKS
                     LFFVDALYLCRLQESSSPQNQADLKTIFLQLEDIVKQGHQIGVHIHPHWLDAEYQEDS
                     NNWNGTNKNHFALSCLDDSLRAETIQKSVEILSPYMDMTRPITYRAGGFYCQPFSLLK
                     DAFAQNNIWIDFSVMRDFTSTGYKNLFAFDFTNPPQELIYRFDDNPLVPNKAGKFIEV
                     SVNSFRLGGLSRIRNSIYFRLNRTSPKMNRMGDGSGSGNFIAHQGDVDYSRYFRSHQN
                     YAVELLNPVLAHSYNQQLKEQKLIHLVSHPKSFTPIGLEGFDLFCQLAAKQKPETDYR
                     TIIRNYLNEPQLNL"
     gene            35037..36773
                     /locus_tag="PK281_00425"
     CDS             35037..36773
                     /locus_tag="PK281_00425"
                     /inference="COORDINATES: protein motif:HMM:TIGR01686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIC family phosphatase"
                     /protein_id="HOY47828.1"
                     /translation="MLSFSELKRNLKKDTSSFKKLEIAILGDSSTQFLTQAIKGYGIE
                     HKLNLSIYDAPYNQLERQLSNKASELYTRKPSFIFLFLSPQKIISSFYSLSKSEQASF
                     ADDYVSRLEQHINIANSELSANFIITNIPEINDQVFGNYGNKNPYSLIFQIRKLNYEL
                     MRLCSSSDNLFINDVASLSNRYGYDQAFSSSLYINGDIIYHLDFLPTLAKNSVDILRA
                     LSGSVIKCVVLDLDNTMWGGIIGDDGIDNIQLGELGIGKAFTEFQRWIKQLKNRGILL
                     AVCSKNNDSTAREVFEKHPDMVLRLEDITIFIANWENKAANISAIRENLNIGFDSMVF
                     LDDSAFERNLVRELIPDIIVPELPEDPSEYLAYLQHLNLFESISFTAEDENRTQMYRE
                     DFNRTALKKVFLDEDDYLRNLELKMNVETVNDFNLPRIAQLIQRSNQFNFRTMRYSPE
                     EIKTISTSPTARCYSFSLEDKHGKYGLISTIILRLDKTQGTAFIDSWVMSCRVLNRGV
                     EDFILNFMMECLEQEGLNTLQGEFIPTAKNSIVKELYAQKGFQPDGNLWIRKLSDYQP
                     ITHFIARTETVA"
     gene            complement(36855..37097)
                     /locus_tag="PK281_00430"
     CDS             complement(36855..37097)
                     /locus_tag="PK281_00430"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOY47829.1"
                     /translation="MTQEDLLVELKPIFTEIMEEDGDKITLATSTNDLENWDSLNHLQ
                     FVVAVEKKYSIRFKSNEIESFRTVGQICEAILAKLN"
     gene            37147..37284
                     /locus_tag="PK281_00435"
     CDS             37147..37284
                     /locus_tag="PK281_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY47830.1"
                     /translation="MNAKLYFSKGKHLKSEKVIRRNTYSIVSIEKLQITHASIGFYKD
                     I"
     gene            37472..38287
                     /locus_tag="PK281_00440"
     CDS             37472..38287
                     /locus_tag="PK281_00440"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOY47831.1"
                     /translation="MNFIQQVIDSSRLLNVLYDNLDKEITGNPNYQKLCDYLSNYLEF
                     NKLSIEEAVEIYTRFISSYNKDCRQFIKTNKYPYELNPELATLSREEYDIILLFSVLF
                     STHRFRIMELIQEKKGSGKALYIGLGPGLELLYTQADFTEKIAYDLAVNPFLNKQFPE
                     VKLNHELYSGQNPDYFDSIYMIELLEHVPDPFQLLKIAYASLKTGGKVLFTTASNIPQ
                     FDHVFNFSPDHTEFESKLQEMGFNILFKEKIQHNYITVDLNSSNHFYVIEKTK"
     gene            38284..39024
                     /locus_tag="PK281_00445"
     CDS             38284..39024
                     /locus_tag="PK281_00445"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HOY47832.1"
                     /translation="MSKKQEKAFQEERSLIIDILRKTRFHQFDPAVHHKQVIPYATYS
                     PWEGDAEFLELFSTIENNTLVDIYRCYELWDLVKNSANIEGDILEVGVWRGGTGAVMC
                     KAAENSNSKVYLADTFEGVVKATEEDTVYRGGEHSDTSDQIVKDLLAKVNVKNAHILK
                     GIFPDDFPEVQIDKLKLCHIDVDTYQSAKDIFDYAWPKMQVGGVVVFDDYGFWTCEGV
                     TKYFNALELADGRKIHNLNGHGMIIKCK"
     gene            39079..39990
                     /locus_tag="PK281_00450"
     CDS             39079..39990
                     /locus_tag="PK281_00450"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOY47833.1"
                     /translation="MAHQNTHTHCILCNAEKLKPLVGFEKAFLTRCSNCGFVFSARIP
                     TNQELITYYEGYGRNDYLSPITIKRYNEILDQFESVRKTNRLLDVGCGIGYFLEVAKK
                     RGWEVYGTEYTDEAIRICQSKGIQMNQGVLDPANYQPESFDVITSFEVIEHINNPQSE
                     ITNISSLLRKGGLFYVTTPNFNSLLRYHLGEKYNVICWPEHLSYYTPSTLNRLMQANN
                     LHKLYIQTTGISITRLITSRKPAPAPAVESNNNEGTEFVSENTQDEKLRNRFESNPLL
                     GLTKKAINGMLTWTGKGDALKGLFRKV"
     gene            40238..41929
                     /gene="ftcD"
                     /locus_tag="PK281_00455"
     CDS             40238..41929
                     /gene="ftcD"
                     /locus_tag="PK281_00455"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018944375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HOY47834.1"
                     /translation="MQQLIECVPNFSEGNNLQIIKQITDEIEAIEGVRLLNVDPGKAT
                     NRTVVTFVGTPAQVIEAAFQAIKKASELIDMSKHTGEHPRMGATDVCPLIPISGISME
                     ETAEYARKLGKRVGEELNIPVYLYESAAVIPGRNNLSEIRAGEYEGFFKKITQPEWKP
                     DFGPAVMNERAGASVIGARDFLIAFNVNLNTTSVRRANSIAFDVRERGRVKREGDPVT
                     GKIVKDEQGNQVHIPGSLKSVKAIGWFIEEYGVAQISMNLTNIRVTPVHIAFDEVCKK
                     ADSRGIRVTGSELVGLLPLSCLLDAGRYFLEKQQRSTGVSEAELIKIAVKSMGLDELG
                     PFEPEKRVIEYLLRDASEEKLVSMSLKAFADETSSESPAPGGGSISAYVGALGIALAG
                     MVANLSSHKRGWDVRWKEFSDVADQAEELKKQLIRMVDEDTRAFNRIMDAFGLANGSP
                     EEKAARKAAIQEATRYAIEVPYKVMQLSASTLTIIHQMADQGNPNSVSDAGVGALCAR
                     TAVMGAWLNVKINCAGYEDKAFVDQTLNTCESIVQGVNQKEASILQLVHQKIANG"
     gene            complement(41983..>44991)
                     /locus_tag="PK281_00460"
     CDS             complement(41983..>44991)
                     /locus_tag="PK281_00460"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOY47835.1"
                     /translation="SYGWDDGNATAIDTGLSAGIYVVHLVDNFGCLADTSFTISEPAP
                     ITTTLSIQPATCNQNNGTITVNAPTGGTSPYEYAIDGGVYSTLSQFNSLAIGTHTISV
                     RDANGCLFQVNGSITMLSYPTQLMMSLTDATCGEDNGVVAPLQVIGGIGPFQIWVNGS
                     DMGVVPVPVTFDSLPAGPFNVELTDVNGCIIDTNVTLQQINGPGSLQYTVQPASCGLN
                     NAIVTLGTINGGTPAFEYSFNGSPFSNQTTYQQQAIGIVTLIVRDQNQCVLDTLIDIT
                     LIPDVALTAQVIQNAQCFNGSDGQAVATIQAGTAPFTFLWTNSETDSLASSLAAGTWD
                     VYVTDALNCRDTAQVVITQPALLTLQISYQNPHCAVSNGIIQADTAFGGTTPYQYQLN
                     GGALQQADMFTQLDVGSYTLQIVDAHGCTSARQAQLAMPSYPTFMTLQSQDAVCSQAN
                     GNARLMGVQGGIAPFQVQWIDSSFTTITDYPVIRSDLDAGNYPLIVRDANGCMIDSVV
                     VLQQFPGPDQMQITVDSSTCDLANGQLAILQVNGGSPAYQYSFNGGAFGSQVIYTNLA
                     PDFYSVQVRDANGCTLDSVMTIAAIPNVQIAPFITHPITCFGFSDGALQADVQAGTLP
                     YAITWLNGPNGASLDSIPAGTYQVSVTDGNGCVQTSSIVLTQPDPVVIDVTGPSYVCE
                     NDEAHLIAQASGGTSHIEVTWPAFNFQGDTLVDTPTATRTYSAVATDVLGCAATDSQQ
                     VTLRLRPQGNITTDVAEGCSPICVNFEVNSTGSAAIQSFNWSFSNGSQGVNAIQKICF
                     TESGLQDVSVDLTDEFGCTATLSAPGAVTVYGLPDARFSYNPDNADIQNPQYQFINES
                     TLASTYLWTFGDGQTSTLESPLHTFPDTGHFTACLRVTTSHGCVDQICKMVEIDPFPT
                     IFAPNAFTPNGDGNNEVFMIRVTYATKFLLEIFDRWGELIYEGTDPLEGWDGTYMGNR
                     VQEDVYVWRATITNTEHIAKQMIGRVTLID"
BASE COUNT        14155 a         8647 c         8912 g        13267 t
ORIGIN      
        1 tgtttttgga agaactcgag atttcctaaa aagaagccag ccaaagtcat ggtagttatc
       61 cataagaacc ctccagctaa atcaaatgga agaaatttct ttttataatt catctgacca
      121 acaccggcaa caaaaggtgt aaatgttcga acaatcggga caaaacgagc catgatgata
      181 gctttggtgc cgtatttttc aaaaaagcta tgtgtttgat cgatgtaagc ttgtttcaca
      241 atgttctttc cacggaattt ccagcctacg actttagaac cagcgaagcg gccaatggta
      301 taattaacgt tatcacccaa tacagcggca aatattagaa gagcaataag tacaatcaca
      361 ttgagttcag aacccggacg agcgcatatt gcaccagcgg caaataaaag tgaatcgccc
      421 ggcaaaaaag gcatcacgac taaacccgtt tcaacaaaaa tgatcaagaa tagaatcgcg
      481 taaatagctg aaccataatc caaaacgagt tgatcgagat gggaattgat atgcaagata
      541 aaatcgagca gagaagttag aaattccata gggcggcaaa attagcttaa ttgacaggca
      601 aatccttcaa ccaatcaaca aataaagcca tagctctagc ccgatgactt atcgaattct
      661 ttgtggtaac gggcaattcg gcaaaactaa gttcaaatcc ttcgggaata aatatgggat
      721 catatccaaa accaccatca ccagtaatct gctccgcaat ccttcctttc acttcacctt
      781 caaatacatg ttctctattt ccatccacat aagcaattac ggtaataaac ctagcttcac
      841 gattggaatg accttttagt tctttaagta ccttttgcat attcgctaaa gcatttcttt
      901 ctatacctgc ataatgtgca gtatcaacac ccggtttatc atctaatgct ttcactaata
      961 aaccagtatc gtcggcaaaa caaggatgac cggttagctg aaaaagtgtt tttgcttttt
     1021 gaagtgcgtt gcccgaaatt gtacctgtag tttcaggaag ttcttgagta aaacctatat
     1081 cagataagct tttcagaatt atttgagaag gcagaattgc ctgtatttct gttagtttat
     1141 gagcattatt actagcaaag acaagttcca tggtggcaat ataccacaca aacacatgaa
     1201 aaagcctatt tttacccgcc aatgcccatc atgtgaaaac agctctcatc actggaatta
     1261 ccggacaaga cggtgcttat cttgccgaat tcctgctaca taaaggatat accgttcatg
     1321 gtattaaacg tcgaagttcc ctttttaaca ccgaccgtat tgatcatttg tatgaagatc
     1381 cgcacattaa taatgcgaag ttttttctcc actatggaga tttgaccgat tcatccaact
     1441 tgattcgaat cattcaggaa gttcaaccgg atgaaattta caatctaggt gcaatgagtc
     1501 atgttaaggt aagtttcgac gaacccgaat acacggctaa tgtggatggt ttaggtacac
     1561 ttcgaattct tgaagctgtt cgactactca atctcaccca gaaaacacgt atttatcaag
     1621 catcaacatc cgaactttat ggattggttc agcaggttcc tcaatcggaa aaaacgcctt
     1681 tctacccaag aagtccgtat gctgtagcta aactttatgg gtattggata acagtcaatt
     1741 accgagaagc atataatatg tttgcggtaa atggaatttt gttcaatcat gagagtccta
     1801 accgaggcga aacttttgtt acgcgaaaag ttacgcgcgg agttgcgcaa attgctttgg
     1861 gtatgcagga taaactctat atgggtaata ttgatgcaaa acgcgactgg ggacatgcga
     1921 aagattatat cgaagctatg tggcggatgt tgcaagttga cgaaccagaa gatttcgtta
     1981 ttgcaactgg agaaactcga accgtacgtg aatttttaat tcgtgcattt gcggaagcag
     2041 gtattcagat tgaattccgc ggacaaggag ttgatgagca agcatttgtg gtgtcatgtt
     2101 cgcaccccga ttatcaatta cctattggaa aagaggttgt tagtatcgat ccaacatatt
     2161 tccgtccaac agaagttgaa ttgcttattg gtgatgcaac taaagctcaa cagaagttag
     2221 gttggacacc taaaatcagt tttgatgaat tggtaaaaga catggttgca tctgatcttc
     2281 agctattccg gaatcagaaa tacattaatg aatacaattc cggcaattaa gaacacggtg
     2341 gaaaaatcgg ataaaatata catagctgga caccgtggaa tggttggttc ggccatttgg
     2401 cgggcattag agaataaagg atacacaaat cttattggag caagttcgaa agaggttgac
     2461 ttacgtagac aatcggaggt tgaagcattt tttgaacgag aaaaaccgga tgtagttgtt
     2521 cttgctgctg caaaagtggg tggaatctta gccaataata tttatcgggc agaatttctt
     2581 tacgataatc tggctattca aaaccatgtg attcatagct cttggaaaaa tgatgtaaag
     2641 aagcttttgt tcttgggctc ttcctgtatt tatcccaaat tagcagagca accattgaaa
     2701 gaggagagtt tgctttctgg ttttttagag caaacaaatg agccttatgc cattgctaaa
     2761 atagctggta tcaagatgtg tgagagtttt cgtcggcaat atggttgtaa ctacattgct
     2821 gcgatgccta ctaatttgta cgggccaaac gataattacg atttaaagaa ttcgcatgtt
     2881 cttcctgcat taattaggaa gatgcataca gcaaaagtca atcaggacgc tcaagttgag
     2941 atttggggaa ccgggtctcc attgcgtgaa tttttacatg tagatgattt agcagatgct
     3001 tgtgttttct tgctagaaaa ttattccgaa gagctttttg taaatgttgg atatggttct
     3061 gatatcagca ttaaagattt agcttatctc gtaaaagata tagttggatt tgcaggagaa
     3121 ttaagatttg accctgaaaa accggatgga acgccaaaga aattgatgga tagcggaagg
     3181 ttgcgttcat ttggctgggt tcctaaaatt agcttggaag aaggaatcaa aagaacttac
     3241 gaagaggtta aacaacagtt ttagaattga tcaagtagtt cttgatacta agaccttcgg
     3301 aaaaacaatt agatttgcgc cttcaaaaaa aaactgtgag ccaaaaatca acccatgaat
     3361 ggaatatgat catccggcct tccggaggat tattcgactt gcggctgaaa gagctttggg
     3421 aataccgtga tttgctgtat ttgcttgtta gacgtgatat tattaccgta tataagcaaa
     3481 ccttgttagg agcattttgg tttgtacttc ctcccatttt aaatacagta atctattttg
     3541 tattgtttgg ggtaattgcc aaggtatcga ctgatggaac aaatcctatt ttattctatt
     3601 taacaggatt gattgcatgg ggatatttct ctgattgcat tagccgcact gcatcgacct
     3661 ttcgtacaaa tgccagtatt tttggaaagg tctatttccc tcgattaatt gttcctcttt
     3721 catctgttgt ttctgcactt gtgaagtttg gagttcaatt aattttgttg attggcactt
     3781 tagttttttt cctcattcga ggcgaaaatg tgcatccaca aatgacgtac gtgtggttat
     3841 taccaattct acttcttatg atgatgttta ctggattagc tttcggtatt attatctctt
     3901 ctttgacaac taaatatcgc gatttaagta atatggttcc ttttgcggtt cagttactta
     3961 tgtatgcaac tccggttatt tatccggcta acgtcattcc tgaaaaattc caatggattt
     4021 tacagattaa tccattaaca ccaattattg agtcaattcg ctatgtattt cttggcagtg
     4081 ataaaataag ctggccgatg ttgggctaca gctttgcttt tacacttgtt actttgttta
     4141 ttggattaat cttattcaat aaaacagaga agaatttcat ggacactgtt taagaaatct
     4201 attcctcaat ccattcccaa gcctatgtct gtagtggtac aagccgatca ggtatcgaaa
     4261 gttttccgat tgggagacgt gggatcttct accataacag aagatatccg ggctacatgg
     4321 tataaactaa gaggtaaaga aaacccatat ttgaatcaag caagtgaaaa cgatcgcacg
     4381 ggtggtgtag gttctgatta tgtttgggca ttgaaggata ttaatttcga agtcaatcag
     4441 ggggaaattg ttggtttagt tggtcgaaat ggagctggaa aatctacgct acttaagata
     4501 ctttcacgca caacaacccc ttcacatgga gagattcgaa ttcgcgggaa agttgctagt
     4561 ttattggaag taggaaccgg atttcacccc gatttaactg gacgtgaaaa catctatctc
     4621 aacggtgcca tattaggtat gcgtcgagct gagatacgag ctaagtttaa cgatattgtt
     4681 gaatttgctg gcgtacaacg ctacatcgac acacccgtaa aacggtattc ttctgggatg
     4741 tatgttaggt tggcatttgc agttgcagcc catctcgaac ccgacatttt gattattgat
     4801 gaggtgttag ccgtgggcga tgctgaattt caaaacaaat gtttgggtaa aatgaaagat
     4861 gtttcggatc aaggtcgaac cgtcttattt gttagccata acatgggagc tgtggccgat
     4921 ttatgttcac gtgccattct tctaaatcaa ggaaagattg ttcaagatgg cgatacaaat
     4981 gaaatcattc gaagttatgt gcaccaaaac atgcatgtaa aactgagtga tgccagtgct
     5041 ttgagcgacc caactgttcg tagaggttta ggacatgttc gttttgaaca aatacgcatg
     5101 ttgaatcgtg ctggagaaga agtaaatgag tttcaaaaag gcgattatat gtgcatcgaa
     5161 atgacagcac gtatcatcaa tccagtaaaa gaacttcttg cttctatcct actccgttca
     5221 ggtaaaacac gcgatattgt tacctcaact cgaagaaata cggttgatat aagtcacttg
     5281 aaaccgggtg atacagtaaa tctccgctac gaatttccat ctctttcact tcgtccagga
     5341 gtgtatgaaa cctattattg gttaggagat aaaaatgcag aaacggcttt tgatgtggta
     5401 gataatttac ttccgccatt agttattcaa atgccaccag actcttcgga actttttgtt
     5461 tccggttatt tcgacgaacc ctttatcttt gagcaattca ctcgctgagt aagcttttca
     5521 cactatgcca ttaaacgaaa aaagaattct aattaccgga ggaaccggat cactggggaa
     5581 aaaactagtg gagataatcc tgaaaaaata tccggatatc aagcgactgg taattctttc
     5641 cagagatgag cttaagcaat tcgaaatggc tcaggattac ccagaaagca aatatccgtg
     5701 tattcgatat ttcttaggag atattcgtga tgcgagtcgc ttacaacgtg ctttcgaggg
     5761 gatcgatatt gtaattcacg cagctgcact taagcaagta ccagcagccg aatacaatcc
     5821 attcgaattc attaaaacaa atgttcttgg agctcaaaat atcattgatg ctgcactcga
     5881 ttgtggcgta aaagccgttg tagcactatc aactgataaa gctgctgctc caatcaattt
     5941 atatggtgca actaaactgt gttccgataa attatttgtt gcagccaata acatcaaagg
     6001 aaaacatgat gttcgctttt cagtagttcg ctacggaaat gtaatgggtt cacgtggttc
     6061 ggtaattcca ttcttcttga agaaaaaagg agaaggcact ttacctgtta ccgatcctga
     6121 tatgacacgc ttcaatattt ctcttgaaga aggtgttgat atggtattat atgcggtaga
     6181 acatgctttt ggaggtgaaa tatttgtccc taaaattcct tcttaccgca ttactgatgt
     6241 agttaaagcc atcgacccaa ctgctaagat tgaagttgta ggaattcgtc caggtgaaaa
     6301 actgcacgaa gaaatgatca ccagttccga ttcgtacaac acagtagatt taggaaaata
     6361 ctacgcgatt ttacctcaag caaacaaccg tattcaacag ttctatatgg accaaggtgg
     6421 taaaatggta gaacctggat tctgctacaa ttcaggaacg aatgattcat ggctcaatga
     6481 gactcaactc cgagaactaa ttgatcaatt tgtttcggtt accgaaaaag cctgattcag
     6541 gtttaaaact aaacccatgt cgttttccga aattgagaca gagaggttga ttcttaagcc
     6601 aatggacgaa tcgttcatta gcgaggaata cctttcatgg atgaatgatc cggaagttta
     6661 tctctacctc gaaaccgggg gaaactactc catggatgaa ttacaggagt atgtaaaaca
     6721 gagtgttaag aagaaaacct tattctgggc tattaccatt aaggataacg gaaagcatat
     6781 tgggaacatt aagattgatc ccatttctca acgacatggt acgggtgaat atggtatttt
     6841 gctaggcgat cgagcatctt ggggaaaagg ttatgcttct gaagctactt caggattact
     6901 caattactgt tttgaaacac ttggaattag gaagataaac ttaggtgtag tcaaggataa
     6961 tgtaagtgct gtgcgcttat acgagaaact tggttttaaa accgaaggtg aatttgaggc
     7021 gcatggcttt tataacggca agtattgcaa cacgttgaga atggccgttt tcaaccccgc
     7081 atttacacat gagaagtaag cttgcccttg gtactgttca gttcggtatc ccttatggaa
     7141 tcaacaacac aacaggaatt cctgatcaag atgaattgaa ttcgatattt tcaactgccc
     7201 agttgaatgg tatccaaaca ttcgatacag ccatcgcata cggcaatgca gaagaacgaa
     7261 ttcgtccatt tttattcgaa ggagctcagg ttatcagcaa gttccctcct gcaagtcaag
     7321 ctcatactct tgcacaagaa ttagatgaat cactcaaacg tttagggcaa aatcatctgt
     7381 atgcctttat ggcacatgat gcagatttat tgctcaaaca gcctgattta tgggcggaat
     7441 taattcgctt acaggctgat aaattgataa tcaaaaaagg ctattcgctc tatcatcctg
     7501 agcaattaga aaaactactc gaagctgatt tcatccccga aatcgttcaa gttccttata
     7561 atttgctcga tcgccgattt ggtcctgcaa tgaagcaact ccacgatatg ggcgtagaaa
     7621 ttcatattcg ttcggtgttt ttacaaggat tgtatttgat ggaactttct aaattgcctg
     7681 aaaaactgca aacaatctcc tccgaattaa aacaacttca cgatttagct ctttccgaaa
     7741 acgtgagtat gtcggcatta gcgttgaatt ttgctattca aaatccacat attgaccaag
     7801 tcgtgattgg cgtagattca tctgagcaat tacgtatgaa tctaaaggtt ctaaattcct
     7861 gggatgataa gaaaaacact atttttagcc aggtaatgca gctaacctgt tcgcacccgg
     7921 agctgctcat cccttccaac tggaaatgaa agttataggt attactcaag cacgaatggg
     7981 ctcttcccga ttacccggga aagtgatgct agaagctggt ggaaaaacac tgctggagca
     8041 ccatttaagt cgagccaaac aatccaagaa agttgactct tggattttag ccactactca
     8101 agagcccggt tctgaagcta ttgctgcaat tggtaataaa ctcaatattc cggtttatca
     8161 tggtagttct gacaatgtac ttgatcgctt ctaccaaagt gtgaaggatt tacgtcctga
     8221 ttatgtggtt agaatcactt ctgattgtcc gttgattgat cctaaattaa tcgacctcgc
     8281 agttgaaacg acgatacaga ataaactaac ctattactcg aattccaatg gtgatacttt
     8341 accagatgga atggatgcag aggtttttac atttgaaagc ctcgaaaatg cttggaagca
     8401 tgccgaatta gctagtgatc ttgagcatgt tactcagtat attcgaagaa atgctgaagt
     8461 caacttccgt tcgtcctatc caaaagaatg gaagaaatat gaatcacttc gtttaacggt
     8521 cgatgaaccc gttgattacg aattgattct gaaattaatt gaaaaatatg gtgatgatca
     8581 agaatggctc acctatgcag atgcagtatt agcaaacgct gattttgctg caataaatca
     8641 acacatcata agaaacgaag gctttatgaa aagcgttttt caggagttag aactccgctc
     8701 aatcacgaac tttacaaaat cggatgaata tcgtgctgaa attcattcaa tcatacccgg
     8761 aggtgcacat acttattcga aaggtgacga ccagtttccg attaaatcac cagctgcatt
     8821 tacccatgga aaaggcgctc atgtatggga tttagatgga aatgaataca tcgactgttc
     8881 gatgggttta ggttctgttt cgcttggaca tgcctttgaa cctgttttag aaaaagtacg
     8941 agcagagtta ctcaatggtg taaacttcca acgaccatcc tcactcgaac gagtaatagc
     9001 caaaaaattc ttatcgcttg ttcctcagca tgatatgatc aagtttgcaa aaaacggatc
     9061 gattgtaact accgcagctg taaaattagc acgtgctaaa acaggtagaa aattagttgc
     9121 ctttccgggc gatcacccat tttacagtta cgatgattgg ttcattggca aaacgggctg
     9181 taacaaagga gttcccgaag aatttagtaa tctatcggta acgttcaagg cttgcaatat
     9241 tgagtcatta agagaattat tccgtcaata tcctggacaa attgcctgtg ttattacgga
     9301 acccgaaaaa caaacttgtg taacgggttg tgcttgtaat ggtcgtcccg atttgttctt
     9361 gaaagaggca atcgaactta cacatgccga aggagcttta ttcattgccg atgagatggt
     9421 ttcaggattc aaaacagggt ttcctggatc gttcactaaa tatggattag aacccgatat
     9481 ggctacttgg ggaaaaggta tcgcaaatgg attttcattt tgtgccttga caggaaagcg
     9541 tgatgtaatg gaattgggag gaattacccg aacgggtgaa gaaaaagtat tcttgatttc
     9601 taccactcat ggaggcgaaa cacatgcgct agcagcagct ttagcaacaa tcgactttta
     9661 cgaggaaaac aaagtggttg atcaaatgca tgaaaaaggt cgccgcttac tcgaacttac
     9721 tcagcatatt gtaaagaaac atgagctaga acacgccatt cagattttac catgtgaatg
     9781 gactccgata ttcttgttta aagatgcaag cggacaggtt tctcctggtt atcgtacctt
     9841 gtttatgcag gaaatgatta gtcgtggagt gcattttcaa ggattctttg ttccatgtta
     9901 ttcccatacc gaagaagacc ttttctattt tgctaaagca ttcgagatgt ctttaccggt
     9961 ttacaaaaaa gcattagaag aaggctatga gaaatattta gttggagagc ctgcaaaagc
    10021 agtattccgt aagattctct gaccttaaca caattatcca gcatggcatc cgatcaggtt
    10081 aaaaaacaag caattccata cggaagacaa accattacag acgaagattt acgcattgta
    10141 tcggaggttc tacaaagtga atatcttact caaggaccgc gtattgctga acttgagcaa
    10201 tcgtttgcgg catacacaca ttcaaaatat gcagtagcta tttcgaatgg aacggctgcc
    10261 ttgcatttat gtgccatggc acttgatgta aaagaaggaa ctcgcgtgat aacctcacca
    10321 atcacctttg tagcatcagc taattgtgtg aaatattgtg gtggtgaagt ttggtttgcc
    10381 gatatcgatc ctaaaacatt cacgctcgac cttcaagcta ctcgtaagct actcgaatcg
    10441 aagcctaaag gattttttca tggaataatc cctgttgatt ttgcaggata tgcagttgat
    10501 cttcaggcat tccgtcaatt ggccgatgaa tttggatgtt ggataattga agatgcttgt
    10561 catgcacctg gcggaagttt cacaacctca actggagcaa aagcacattg tggaagtggc
    10621 gaatttgctg atttggccat tttttctttt cacccggtaa aacatatcgc tgctggtgaa
    10681 ggtggaatga taacaaccaa cagcttagaa ttatacaacc gactgctcaa gcttcgcacg
    10741 catggaatca ctaaagacga aagcttgttg attgataatc acggaggttg gtattatgaa
    10801 atgcaggagt taggctacaa ttatcgctta acagatttcc aggcagcatt agcttcaagt
    10861 cagttacaac gtgcagatgc cggattggct agacgacaag agattgcaga tcgctatcaa
    10921 caagaattag ctggattgcc aatcgagcta cctcaagttg caaccggagt gacgcatgct
    10981 tggcatttgt atgtgattaa gacggagaag cgaaaagaat tatacgatta cctaagaact
    11041 cagcatattt tcacccaagt tcattatata cctgttcata caatgccgta ttacaagcaa
    11101 tttgggtgga aagaaggtga ttttgaagtg gcagagaaat actattccgg ttgtttgagc
    11161 ttacccatgt ttccaggatt gactgatgaa gaacagggat atgttattcg atgtattcgg
    11221 gagtttttta agagtgaagc aggagcataa gcttataaat cattgcaaat aagctcagaa
    11281 ctaacgagca ttcaggaatc agaaaagaat agaaatgatg aaggaatcag cttcgatgga
    11341 aattaccaga atagcgaaat cctattcctt gcaaggctat aaggtgttta cactgagcat
    11401 tggtgatacg cattttggtt tacctgatcc tatcaagcta cgcttaaata aagccattga
    11461 agagaataaa acacattacc tcgattcgat gggacttcca gctttgcgca tggacatttc
    11521 ccgtttcgaa tttcaggaag catatcttca agatgaaatc ctgattgttc caggtgttaa
    11581 acaaggctta ttctattttc tagaagcatt tgaagggaag aaaattgcca ttttagaacc
    11641 agcttggtta ggctaccatt ctatttgcaa aatgactggg aaagaaatca tcccaataaa
    11701 catgaagaaa gagggctggt tagataagct tgccagtatt tctttcgatg cattgttggt
    11761 ttgtacgcct aataatcccg atggaactgt tttctctgag tcagaaataa aacaacttgc
    11821 ttcatattgc gataaaaatc aagcttgctt aatcattgat gagatatacg cggagtacag
    11881 ttttgatcat tccgtgaaat cgatgttaaa accttattac tcccaagaaa atgtggtagt
    11941 aatgaatggg ttttcgaaag cctatgctgc aacaggattg agaattggct acgtggcaac
    12001 acataatccg gtttggaata agcgaatgaa tgcggtacat cagaatactg caacatgtgc
    12061 taattctttg gctcaatttg catttctgaa ttatcacgaa agtcatagta ttgttcagca
    12121 gtatgctagc tattaccgag aaaacagaga tttagtttgt gagattattc ctgagttaga
    12181 agaaatgaaa cctcagggag gattttactt ttttattgat ttgaatcgat tcggtatttc
    12241 ggatgcggaa gaattttcgc gcagaatact gactgatata aaaattgctt tagttccagg
    12301 atcagcatac ggtaccggtt tcgattcgta tgttcgtctc tcctattcgc tcgaccgaga
    12361 tgaattaaga gaaggaattc agctactaaa aaaataccta caggattatg aatcagaatg
    12421 acattaaatc ccggatgatc agccagatcg aacggaacag aatttctagt gtggaagttg
    12481 ccgatgcgct tggtaaaaca ggtgtactca atggtccaca gattcttact cccggaaaat
    12541 tttgtgtggg tgaggttcaa tacatctacg gtcacagtga aacaaactgg cccattcacg
    12601 aacaaggcga gcatttgaaa gatggtggaa ttttgtacgt cgacacgttc aattgcaacg
    12661 ataaggctgc atttggcgat ttggtgtcga agtactttat gctttataag cgcattggcg
    12721 gaatggttgt caatggatta ttgcgcgatg ctcatacgct ccgaaaagag aattatgcta
    12781 tttggtgttt aggcacaaca ccgctaggat gcgtaaacgt taatattcca gttccacccg
    12841 atgtagaagc tgaagcaatg aaacgcaaag cttacttcga cggctccatt ttggtttgcg
    12901 acgattcggg atgtaccatg attgccaaag aactcatcac tgaagcaact ctaaagaagt
    12961 tggaattcat ggagttacag gaagacatct ggtattattg tctcgacact ctaaaatggt
    13021 cgacatacga aactgtgtgt cagaagaaat acctcagtaa taaatcagta cttccaactg
    13081 tacttgtcga aaagctgaag gaatatgacc tataagcgaa ttcccgtatt tgacacgctt
    13141 tcacacccaa ccctaaacgg taattggttt gggaaagatt gtgggaatac gtttgagaat
    13201 ttgaaaaagg aatttcatgc taacgacatc attggcactt gcgcagtttc tttgccgcct
    13261 atgaccaatg aggaattgca atcgttttac actcgctgca catccgaaaa agagattgtt
    13321 ttataccctg ttgcggcatt caacttctcg gaagaaaact acgaggacaa gattcgtttt
    13381 atccgcgata ctggctttaa agcagtaaaa atccatattc gactttcggg tttaaaactc
    13441 gatgaggatt ttgataaact agctgaagtc tttcgtttat gtgagcaatt tggtttagtc
    13501 gtgtttttct gcacctattg tcacgcttcg attgattata caccaccaca tccgctctcg
    13561 tattacctta tttctctgtt gaaacaagct cccaaactca aggtaatctt gttacatggc
    13621 ggtgatgtga gtttgctaca aatcagtcaa cttgcccgtt tcaacccgaa catactcatt
    13681 gatttatcct acaccatgct caagtttgcg ggttcgtcgg ttgatcagga cattgttttt
    13741 ttgatgaata atctcgaccg gaaaatctgt ttaggttcgg attatccaga ttatacgatt
    13801 gggcagttta gggaacgagt ggatgggtta ctaaatcaag taattgaccc agaaaaaatc
    13861 gaacgaatct gttcgggaaa tatccaatca tttttagaga tagggatatg atttgtttcg
    13921 tgtatgagct aaatcccagt attggaagtg ggcattttat gcgttgcaaa agtgttcaag
    13981 aaacgctgat gcaaaaattt gggattcctt ctcaactcct ggctgaaaca gatttcaatg
    14041 catccgattg gcatcaggca attgtcgtgt ttgatttatt cgacacagca aaaaccacta
    14101 agtttcttcg tgacacttcg aatgaaaacg ttaaaatctg cttcgattat ttctccaacg
    14161 aagagcaacc cgatttaaac attagtgtcc tggaacaatt tcatgaaaag cgttctcacc
    14221 cgaattatac tgggctggat tactgtgtgc ttcgccctga ttttctgaaa aatcccgttc
    14281 taaaagagaa gttaaacgaa gtgtttgttt acatcggtgg aaatggattt cctgaagtgg
    14341 taagaaccat cgcggataaa ttttctgatt cgccctttca ttttaatttg attagaaatc
    14401 agaactcgct cgattgtggt ccattgccag ataatttctc ggtgcatttt ttaccagaaa
    14461 acctgataga actgatgaac cgaagtacat ttgccatcac atcaccggga ttggcaacta
    14521 tggaacttct gtatttgcag gttccttctg ttttatgtcc tttgatcgac tttcacgaat
    14581 acttcagcga ttttctactt caagaagatt tagctatttc gcgtttagat gatttcgata
    14641 gtcgattact ggaaaatcta ccaagaatgc gcgaaagtgc tcgtaaaaaa gtagatggca
    14701 aaggcgtaga acggattata catttaatgc tcaatgcata tgaagaaaaa atgggtagtc
    14761 gttatccctt gtgggcttga ggttattcca accatcgaag cggctaaaaa acaaggatat
    14821 tcagttgccg gagtagatca aaacccagag gcaccagcta tgaagttttg tgacctcgga
    14881 attgtttctc ctttgaacga ttttgagcac atcttatatg tgttgcaaac caataagatt
    14941 gttccagagt gcgtcattcc aattgtttcc gacaaagcgg tattccctgc ttatatgttg
    15001 aatttgcaat ttggaaaaac gcctatcaac ccaaatattc tagcatttta ttctaaatcg
    15061 gttttaagaa acaccctgaa tacattgggt ttcgacaatc ctttttattt tgtagttgat
    15121 aaagaaggag atttaaagca tgttcctttg aaatctaaac tgattatcaa accagatgat
    15181 tcctctggaa gtcgtggaat tacgatttta gaaaagccca atgcaaaaaa tctgaaagaa
    15241 gcttatcagt ttgcattagg attcagtgcc aataaaaaag cggtaatcga agaatatatt
    15301 ccgggagaag aatatatggt tgattgcttt gttcatgaaa aggaagtaaa ggcattgctg
    15361 gtaagtcgta aagaaaagat tcaggataaa gtatccatct taattcaaac ggtaaatgag
    15421 caagagtttc cttatgctaa actgaagaaa ctaattacta aactttccaa ggcattaact
    15481 tatccgggcg gaccattaca cattgaggta aaacttcaag atgggaaatt ctacatcctc
    15541 gatttagcgg cacgtggagg aggatttagt gtgtacaatt tctacgttaa aaagtgcctc
    15601 ggatttgatt ttgttcaagc aactttggat gttttccttc agaagaaatt gagtcagaaa
    15661 tgcggcacgt ttcgcgaagg cttgatttat tttatcactc ctgaaaaggc tggaacgctt
    15721 aataatttga ctacttcctt ccaggtgaat ccgaaaaaag atgtgcttat caactattac
    15781 tttactaaag gacaatcggt tagtttagat gtaacagatg gcaatcgttt ggcgggtatt
    15841 tattgctttg ccaagaataa acaagctttg gaggatttgt tcctcgaagt taaatcgtcc
    15901 tttcaccaaa catatgcgtg atgaataaaa atgaaattca gcaaacgata gatcgctaca
    15961 acaagcgact tcaagaatat ggtgaaaccg aaaaagcttt aggctggggc gacaaaggtc
    16021 gttctcgact tcgttatcat gctttgtgta atgagttcga cttaaagaac acaactgtat
    16081 tagatttcgg ttgcggattc ggagatttat acgcttacat ccgcgataac attagtcccg
    16141 attttaaata tattggaatc gatatcaatc caaacctaat tgaagtagct caaaagcgct
    16201 acggaagtca ggccgaattt catgttgttc cttatgaagt aagcgaatac cttgaacaac
    16261 accaaattaa ggcagattac attctaagct caggaatttt caattttaaa ttggacgata
    16321 acattggctt tatcgaatcg acgctacatg tatttgatcg attttccaca cgcggttttg
    16381 cttcaaattt cctgtcggat aaagtgagtt ttcaagcaga atctaatttc cattccaatc
    16441 caggtcaaat actggagatg cattataaat acagtaacaa tgtaacgtta cgaaacaact
    16501 acatgccttt tgaatttagc gtaatggtga ataaacacga tgcggtaaat aaagatatga
    16561 atgtgtatga atcgcatctg caatacattt gacacccaat tttcacctca ttgaatcaac
    16621 aactatggaa gatattcgaa tcggaaaaca tttaattggc gacaatcaac ctccgtatat
    16681 cattgcagaa atgagcggaa atcataacca gtcaattgag cgtgccttcg aaatagttga
    16741 tgctgctgct gctgctggcg ctcatgcgat caaactacaa acctacacag ccgatactat
    16801 tacaatgaaa ggcgtgttaa ccatcaatga taaaacatca ttatgggatg gatatgaatt
    16861 gtatgatttg tacaaagaag catacactcc ttgggaatgg cataaagcta ttttcgatcg
    16921 tgcacacgaa aaaggtttag atgcttttag ttcaccattc gatgagacag ctgttgattt
    16981 cctcgaagaa ttaaatgttc ctgcttataa gattgcatct ttcgaaaaca gtcatattcc
    17041 actcatacgt aaagtagcac aaacaggtaa gccagtcatt gtttcaacgg gtgtagctac
    17101 gattgcagaa attgatgaat gcgttcgtat gcttaagaaa cacggatgta aagacatcat
    17161 cttgcttaaa tgcacgagca cctatccggc cacacccgaa aatactaacc tcaacaccat
    17221 tccacatttg agaaacctgc acaattgcgt ggtaggactt tcggatcata cgatgggaat
    17281 cggaacatca gtagctgcgg tggctttagg agcaagagtg atagaaaaac acttcacctt
    17341 gcgtcgagct gatggaggag ttgattcagc attttcactc gaaccggaag aattagccag
    17401 tttagtaacc gaaacagaac gtgcatattt gtcgcttgga agaatccaat atggtgtaca
    17461 ggatgcggaa gttaattcgg tgaaaggcaa gcgatctata tatgtttctt cgccaattaa
    17521 gaaaggtgaa aaactaagcg aacaaaattt gcgaatcatt cgaccagctt tcggactaga
    17581 cactaaatac tgggatattg taataggtcg cgaagcgaaa acggatttgg aacctggaac
    17641 tccattatct tgggatttaa tctgatttcc aataatcaga aacagtacaa tgctcaacaa
    17701 ttgaaaggga aactatgaaa gtggttattt ttaattcgaa cctgctttgg ccctcgcatt
    17761 atgaaacaga gctggaactg atttcaacac atctagcaca tggagatgag gtaactcaaa
    17821 tttattgtga tgctcaattg cccaactgtg accttaatcc cttttttgtc ccttctgttt
    17881 gcgacgaatg cgttcatttt aggaaacaag gttaccaagc gatttcgggc gattttacta
    17941 aaatacgact tcccgaatta tccgaagctg acaaaactcg aatagcccaa cttccccttg
    18001 aattcaagtc attagaagaa attcaagctt tagaattaga tggctttgac ttaggatatt
    18061 gtgtagcttc ttcgctaatc agcattattc gtgaccctaa acctgatttc aaactttatg
    18121 cacccacaat aaaatcgttt ataaccggct cagctgctat attcttaatc ttcaaaaaat
    18181 ggttgattga gaataaaccc gatttagttt acacgttcaa cggacgtttg gcacatacta
    18241 aacccgtgct tagggcttgt caacaagtag gaattgagtg taaaattcac gagcgaggtt
    18301 ccataaaaga taaatattcc cttacgctaa acacatctcc ccacgaccgt aagtatgtga
    18361 aagctaagat tatagaaacg tggaatcaag caaatccaga ggagcgactt ccgattgcgg
    18421 aagatttcta caaatcgaaa ttcgctggaa cagataccaa ttggtattca tacaccaagg
    18481 aacaaataga tgaattgcca gaaaattggg atcccaacaa gaagaatgtg gtgattttca
    18541 attcttcaga ggatgaattt gcatcattag ggaaagaatg gaaaaatgtt atttatcctt
    18601 cacaagctga tggtattaaa caaatagctc aggaattatc gaatgattcg aatgtgcatt
    18661 tttatttacg ggtgcatcca aatttgagga aagttgataa tgcagataag aaacgtttat
    18721 acggtttagc tgccccaaat ttgacaatca ttccagccga atcacctatt agttcctata
    18781 agttgatgat gatggccagt gttgttttaa catttggctc aagtgtagga attgaggcaa
    18841 cttattgggg gaaagtatcg attttggcag gaaaaacttt gtacaaggga cttggttcca
    18901 cgtacgaacc agaaagtcat tccgaagtaa ttgagttgct tcgttcagaa ttagaaccca
    18961 aagatcgaac gggagctttg atgtttggat actattataa atcacacgga ataccatata
    19021 aatactatca acccgaagat atcgccaatg gtacaataaa cgggttaaga ttagttcgaa
    19081 ctaaaaattg gaaaggtaaa ttcaaagatt ggttttttaa aacacctgcc ttgcaattca
    19141 tttccgaacg tgtttactat aagctgcgtt acgagcgatt aagaaattac cgaaatgact
    19201 gaactggttt cgattgtcac gccagtatat aattgtgttg cctatttgcg cgaaacagtg
    19261 aaaagtgtgc aagagcaaag ctattccaat tgggaaatgc tcttagtgga cgatggttca
    19321 actgatggtt cagctgaatt ggttgatcaa ctcgctcgag aagattctcg tataaaagcc
    19381 atccatcaac ccaatgctaa acaaggaaag gctcgaaata acggaattag tcaagctact
    19441 gggaattgga tagccttttt agatgccgat gatttatggc cagctaacaa attggaattc
    19501 cagttacaaa aaacgttgaa tacctcgttt ggactaagtt ttacggatgg attcatttgt
    19561 ttgaataatg atatgaacct tcgagcgcat cgttttggtg tggttgatac aacgtatgaa
    19621 ggagaatcgg gaattcgagc atttcatgaa caaaatagaa ttccgacttc tactgttatc
    19681 gtaaaaaaag aattgcttat ccgtacagga ggttttccag aacatttatc cgttcaaaat
    19741 tgcgaggatt atttgctttg ggtgaagttg ttaaaagaag gcgctacgtt tattagcata
    19801 ggagaagcat tgctttatta tcgggttcat ccaacttcat ccacatcaca ggaaacaaaa
    19861 gcactattcc ctgtaattga agcgttgatg agtatggcta gcactggtac tccagaactt
    19921 aaaaatcatc taggtgagaa gtttcgtcaa ctaatctttc gattaaacga attaggtgaa
    19981 ttgaatcagg cagctggaat tattccacgg gtaattcctc aattgtacca aggtgttcag
    20041 agagctcttt tgctttcttc ttggaaatta agtccggcaa tttttatacg agtcttttgg
    20101 agaatgacag gtgggcgtat aaaaaattga ggcaggcacc tgaaagatgt cctgccccaa
    20161 tacctaaatc aaactaacgc tacgctaaac taacaggttt gataataaat ggttgagcag
    20221 cgccactaaa tttatcgcaa cacactataa ttcagccgat tatttttttg aatgattctt
    20281 atcaaggatt tctgcaagtc agcaattaag gttctgtaaa aaaaagaaaa gggaaatacc
    20341 tacttttgag cagaatcaac acagagaaat ggcaacacaa ggacttcggg aacaaattgt
    20401 taaggtactt tcaggtaatc cgataactga gaatctgata ttgaaacctg tcagaaacta
    20461 tcgaatccgt aagcattacg aggagttgat gttgttgaag gatttaagtc ctgaacaaag
    20521 acagcattgg gcagatcgga ttcggattac aaaactgagt accgacaatc agaagataaa
    20581 taagattcca acttcaggaa aactgataga tgggaaatta cacatgcata atggattggt
    20641 catagatcca ctcagttatt atggcccgcc aatgttgcgt ttgttgatgg agaatcatgc
    20701 tgttcatgag ccacaggagg aatatgtgtt tcaagaagtt ttgaaaaaca tgaaacctgg
    20761 agcggtaatg cttgagcttg gctgttattg gggattttat tcgatgtggt ttgcgagtaa
    20821 agtaaaaaac gcgaagaatt tcttaattga caatggcgat ggaatagctc gtgcaaaagg
    20881 aaactttgcc atgaacaagc tcaatgcaac atttatggtt ggatacattg gtaaagatgt
    20941 agcaggttca gagccacccg ttactaatgt tgaccgaata tgcgagcaac aaaaaattga
    21001 tttcgtagat attcttcatg ccgatatcca aggatttgaa atggagatgt tggaaaccat
    21061 gccgatcctt accggtaaaa aaggcattgg atatatcttt atatcaacac attccgaaga
    21121 attgcatagt ggatgtattg cttggttgaa agatcatggc tacatcatta tttgtgatgc
    21181 caatttggaa cacacgttct ccgaagatgg attaatcgta gcgcgcgacc cttcttaccc
    21241 aggaattgaa ccaatggcga tttcacaata ccaacgaacc tgatcttaaa aaataagatt
    21301 cgtgctcatt tccatttgca ttcctaccta tcgccaatta ccgatcttat tcaatctatt
    21361 aaaaaccata gattttcaga cgtttcgcga tttcgaagtc attatctccg atgatagtcc
    21421 tgatgatgaa ctcgagcaag ctatattatc ggccaattgg actttcccga ttcgttatta
    21481 cagaaataat ccggcaaaag gttcacctca aaactggaat catgttattt cattagctca
    21541 gggagaatgg attaaaatca tgcatcacga cgattggttt tatgatgccg attcattgaa
    21601 atactttgct gaggctatca gccaaaatcc cgaaacagac ttcttttttt gtcaaagttg
    21661 ggttagtaat ggaaagaaaa acgaagatta catctatcga cctaattcag acaaactgag
    21721 gaatgtctcg aagtttcctg ccgatttatt cgaagccaat ctaattggag caccttctgc
    21781 aactatgtat aaaaaaacca tgaagttgca gtacgatagc aaactcatgt ggcttgtaga
    21841 tattgagttt ttttgtcggg ttattcaagc ttacaaaatt cagccaattg agcgtggatt
    21901 gattgtcaca agcgaatttc aggcgcatca acttacttat acacttcgcg ataataaagc
    21961 aaccgaaata cgagaatatc tttattgcta cgaacaatta tgtggcattt ttaatcccgt
    22021 taatcgagca atattaagac gacgaataat ccacttgttt ttacaatttg aggtgaaatc
    22081 aattaaagag ataagagatg cgggatatat tggaagaata cctggatttg ttaggcttta
    22141 tacgtcgcta atgaatttta atgcaggatt agcacagaaa gtaataggta aatggactta
    22201 tctacaattg aaacattatg attataactg agctaattag cggtttaggg aatcagcttt
    22261 ttcaatatgc agcaggaaaa tcacttgcgt taaaaaagga caccattctt aaaattgacc
    22321 gcacttggtt tgatttagaa tttgaaaaac aaacccatcg gacttacggt ttagatcctt
    22381 ttgcaattca atcggagata gcttcacaag cagaaattga tttgtattta cgaccacaat
    22441 cgagtaatat attagatcgc attcagaata aaataaaatc gtaccagcct tattataagc
    22501 aacgtgttta tttagaacct cattttgaat tcgacactaa tttttttaaa acaagcgagg
    22561 atgcttattt gagtggatac tggcaatcgg aaaagtattt taaagattat gcttccatca
    22621 tccgccagga attccaattt aaacttccac ttcaggataa aaacctgcat tggcagcaat
    22681 taattagttc ttctaatgct atttcgcttc atgttcgacg aaccgatatg gtgaataatc
    22741 ctgatgtggt taaaactcat ggctcatgtg gtctcgatta ttaccaagca gctgcatcaa
    22801 aaattgcaga agggcttgaa cagcctgtat tctttatctt ttcggatgat cccgaatggt
    22861 gtaaagccaa tttaattctc gactggccgg tacattatat cgataataat caaggagatt
    22921 ttgcctatat cgacatgcaa ttaatgagta tgtgtcagca tcatgtaacg gcaaatagta
    22981 gttttagttg gtggggcgct tggttaaatc cttctcctag taaacgggtt atcagcccta
    23041 taaactggtt tgctgaccca agcaaaaaca catccgacct gattcctgct acttggcaac
    23101 gcatataggc tcttgtagct tttcgtgatg tttcatttgt ttgtactttt aaccctttcc
    23161 taatctatgt cacagccttt agtttcggtc cttatgcctg tttataacgc aggaaaagat
    23221 ctagctgagg ctatccaaag tattctcgat caaacctata caaactttga gtttatcatc
    23281 gtaaatgatg gatcaactga tgcgagtcga gatgttattt cgaatttcaa tgattcccga
    23341 attcacctta tcgacaatga ggttaacaaa ggattgattg ctactctaaa ccttgggatt
    23401 gatgcatgta agggcgaata tatagttcgc atggatgctg atgacattag tttgccagag
    23461 cgtattgaac atcaaatagc gtttttagaa gcgcattctc attatggtct agttggttgc
    23521 tggtttgagg actttgggga tcaaattcaa tcgaaagttg ttcgatacag cgatgatgat
    23581 acgtacattc gaattcgtca tttatatcaa acccatattg cacatccaac ggcagtttgg
    23641 cgtgcatcta tcataagagc acatcagtta aagtttgatc ccgcatttgt tcatggtgaa
    23701 gattatgaat tttgggtgcg aatgagtgct tattgtaaaa tgtcgaatgt gaaggaagta
    23761 cttgttcgaa aacgcgacca tcctcataat gtgacaaaca agtatgcgca aatcatgcaa
    23821 gacacgtgtg ctcgtgtgaa gcaaagacag ttttcactta tgggcattaa ctgctctaca
    23881 gatgaagtga atttgtatag tcgttttgct gatggtgaat ggcgcttttc tcctccacaa
    23941 atggatcatt tagagacctt gctggaatca attttcaaag cgaataagca cagtaaattt
    24001 atccccgttg aagcatacaa tcgatactta gcagaaaagt attatcattt atgctataac
    24061 aatcccgata tttccaaaga aggatggaaa cgtttccgaa aatcttcttt cagccgctta
    24121 tttaaaggta aatttcagga taatttaaaa ttccggatta aaacgttgct tcgctgaatg
    24181 gaacaggaaa aacctttttt cagtattacc attcctgctt ataatcgcgg ctatattttg
    24241 cctgaaacta ttggtagcat tcagtcacaa tcatttcaga attgggaagt cattgttgtt
    24301 gatgatggct ctacagataa tacacaagaa attattgctc aattgtcgct agccgattca
    24361 cgtatacggt atgtgtatca acaaaatgcg gaaagaagtg ctgctcgtaa taatggagcc
    24421 caaaatgctt taggaacata tttgatgttt ttagatagtg atgataaata tgcagatggt
    24481 catctcgaaa aattagcagc ttatattgaa tccaaaaaga atcctgttgc cttattcttt
    24541 accaacctaa gttatctaac cgaaaatggg attgaaattc ctgaaattcc ggtgatgcaa
    24601 ctaggcaagg aattcgacta tttgcttttg aatcctatta ctccgtcgcg tgtttgtatt
    24661 catcgcgata tttttaacga tttcaaattc gaccctaaga ttgtaattgt cgaagacatg
    24721 gttctttggg tttgtatagc aactaaatac cctgtttttc aacatacaga aaactcactt
    24781 atatatagaa ttcacctagg aaattcggtc gatttgagtc gtaattccta ctatgatcgt
    24841 tataaaggac tacttcgact ttttaatgat gaagaatacc aagannnnnn nnnnccaaga
    24901 aaatagctac tcatataaaa aagcacctat tggccgaatg ctccttcaat atggccaggc
    24961 attttgagta cattaaaaaa ttcggggaaa tgaatcatat gcttttgcta tcctttaggc
    25021 atcgccccgg ataccggaat aaagaacgct tgtatatgtt tttgagccac ttcccattaa
    25081 ctgcacctat tctaaaaaaa ggtgatacta atggaagaac tcaatcaaga ataccgaata
    25141 gttagggcaa gtgaagataa cattgccgac ctctcccgtt tattcaaaga atctcgcgga
    25201 atacatgttc ctgtagacta tttgaaacgt aaatacaata ctgcctacac aagtaagtcg
    25261 tatttcgccc actttgccta ttctgcagat ggaactccag cagctttctt ttgtctattt
    25321 ccctgctttg tcagaattaa tggggagaaa aaattagccg gacaatcagc cgatattatc
    25381 acacacgttg atcatcaacg aaaaggatta ttcggtttgt taggtagagc cactgaagct
    25441 ttagccaaac aagaaggcat gaattacttg ttcgcctttc ctaatagcaa ttcattccct
    25501 ggattttata gaagtctgaa ttggcatcat ccgggaaact ttaagattta ctcattcccc
    25561 atatcaggaa ttcctattta tcgaattctt cataaactga aattaggcaa gatatactcc
    25621 ctctggaaga gttttgtaat aagtatgttt cggtcaaaag aagaaacgtt tattggtgct
    25681 gatttcgaaa gtggcgaaaa tgtttgctgg cgcgatgaat ccttcttttc ttataaaaaa
    25741 tataacgaca atttcgtttg tgagttaaat ggatttagga tttgggcgaa ggtagaagga
    25801 gcattaattg ttggagatat tagtcgatcc aatatgccga ttgaaaaact tataaataag
    25861 atctctcggt tgtgtaagtt gttaggactt gattcctttc aaattgaagt gagtgaaggc
    25921 agtttttacg acaagctact tcgcgataaa catgcgtttt tagagggagt tccgatagtt
    25981 ttcaaaaact tggatatacc tgagcaaaaa ctatctttgt cgcatacagg cgctgatgca
    26041 gacgtgtttt aaaacctgtg ttctacagga aacatgttgg ccagaattca gaataagctg
    26101 ggccgtattg ggaatgattt cattttcaac actgggctat ttgcctctaa caggctaaag
    26161 gaaacagatc ataccatatt gatgtatcat ggagttgata cggttggtgg taccgatttt
    26221 aatagtagac atacttcaca ggctgatttt tacaagcatg ttcagtttct aaagaaatac
    26281 tgccatatca tttcgttatc tgattttttc gagaagcgat ttataagtgg aaaaccgaat
    26341 attgccctta cgttcgatga tggttacagg aataattttc tgtatgcaaa acctgtctta
    26401 gaagctgcta aagtcaatgg cacattttac ataacaggac ttaacactag taattcggat
    26461 atactttggg ctgatttctt aaacatcgcg tccaaattaa cagagcaggt aatttcaatc
    26521 gatggagaga aattcgaaca gaaaaatggt atttaccaca gtttagattc gggtaaaaat
    26581 ctttattcca ttatcaaaga cgaaaaggct ggaacagcct acaagcaatt aatgaccgag
    26641 tcgtttattg atttgtacga tttcaagaaa gatctggctt acgatgatta ttggaaacta
    26701 atgactgatt tagaaatccg gaattgtgga gattccaagt atattgaagt tggctcacat
    26761 ggatttcttc ataataattt gggaaccatt tcaattgaag acgctacttc agaattgcaa
    26821 aattcaaagg aatacctcga aaatatcatc caaagtaaaa ttgtttcggt aggataccca
    26881 gatggttcat atacccgtac aacattagat gccagtgaag atttaggttt tctttatcag
    26941 acagctgccg aaggctttcg ctttttagaa gatgaaacag acaaccgcat tcgtgaccgc
    27001 aaaggaatgt acacctgtga tacctgcgcg aatcaattgc tagttaatct gtaatctttt
    27061 ctgttcatag aatagacaat gagtaaggag atttagcaat ttctaataat gaatttctta
    27121 atcagaataa actagcttta gtaaataact caagtttaga tttgctgtac acgctgaaat
    27181 taaacgactt atgcctcttt accatttata cagaattaaa agattgaaac atttgattta
    27241 aaataaatca gtagtgatat cgatatttat taatcgtctc attgtaaaaa tttgaaaaac
    27301 atatatttgc cgaatcgtcg aaatgacctg atatcaggat gaaaatactg tatgtaattc
    27361 ctacaatggg ttgtggagga gcagaaattc tcctcggggc aatagcacgt aatttagtca
    27421 agaaggggca tgaagttcac ttagtatgcc tcgaacctca tcacgaaact tggcccaatt
    27481 accctgataa agaagccatg ttagccgaag ttccggtgac cattattggt ggcagtgttc
    27541 gattccgatt cttaagaaaa cctcaagttg ataccggtgc tttcgtaaat tatgtaaacg
    27601 atttcaagcc cgatattatc cattcgcatt tgtacctatc tgaactagtt tctcgttcag
    27661 ttgtatttag ccatatcaag tatttttccc atgggcacga caatatgcca caactgcgtg
    27721 ggcttcattt taatacactt tggtctaaac cactacttgc caattattgg gaaagaacat
    27781 ggttaataaa gcaatacaag gcttgtaaca atcattttat tgctatttcc aatgatgtaa
    27841 aagcctattt ggaatctgaa cttcctgcat ttcgaaattc agtaatctat catcccaatg
    27901 caatagatat aaaacgcttc tattttgctc gcacatttga gcctgttcaa ggtgtgttcc
    27961 atatggtttc gatagctaat ctggtcgaaa aaaagaatca cattttattg atagatgtga
    28021 tggatgtttt aatccgcaaa ggttatcgag ttagtatgga agtattgggt gcagggcctt
    28081 tgatggaaat gctacaacaa aaaacacgag agaaagggct tgaaaaccac ttgtttttta
    28141 gaggaagtgt gggtgatgtt cctgaacgtt tgaaaaatgc acatttgtat gtgcatcctg
    28201 cttggtacga accattcgga ttggttttat tggaagcaat ggcctcggct ttaccggtag
    28261 tatctcttga tggatttggt aaccgcgaat tgatgaaaga aaatcagaat ggttttatgc
    28321 taccaactaa ggctagtgct gaggagtttg ctgctaaaat cgcctatttc attgatcatc
    28381 ccgaagagcg ccaacgaatg ggcctttttg ctcaagagtt ttctaagcat ttcgatatcg
    28441 accactatac cgatggctta attgaaattt atcagaacaa ctcaaaagct actacaactc
    28501 attaatttca cgcagttcag aaggagaagg ataggaaatg agcaccgctc tatgtttacc
    28561 ctgaaacaca aacatacttc ctgcagccac agccgaagca cctgcttgaa tagcttgttt
    28621 gagatgttgt aaatttcctg ctccgcccga agctattaca ggaatactta ctgcatgtgc
    28681 tacttctgaa atcgttttca agtcaaaacc ttccatcatc ccatcccgat caaccgaatt
    28741 caacactagt tcacctgcac ctttttgctc taacatttga gcaaactcga tgagtggaat
    28801 atttagcgtt ttagaagcat tccgaacagc aacccggtat tttccccaaa tatctttctt
    28861 cacatctatt gctgctacag tagaggaact tcctaaaaca tcggcagttc gagtgattaa
    28921 atctggatct ttttccaatg ctgaattaat agccaccttc tcaatcccag ctttgataac
    28981 agctcgaata tcttcaagat ttgacaagcc tccaccataa cataagggca taaaagcctc
    29041 cgtagctatt tcagaaatgt atttgaaatt aggtcttctt ccttgtgcag ttgccgtgat
    29101 atctaagaat atcaactcat ccacttcttt atcattaaat atcctgactg cattcacagg
    29161 atcacctacg taaacaggct ttttgaattt ctgtgtttta accagacttc cctctagatt
    29221 acacaacaat acaggaataa cacgggattg atacatagtt atcgcggctc tgaaaaattg
    29281 tgtagtaaag acatcccgta tttatgactt ttctcaggat ggaattgaac gccagctatg
    29341 ttttcatgct caaatgctga gcaaaacgta taaccataat ccgaaagaag taattgatcc
    29401 gcttcgtttt tcaaatggaa atgataggaa tgaacaaagt aaaatcgagg attctcgggt
    29461 aatacatcta caagtggact ttgttttacc tgccggattt gattccaacc catattagga
    29521 attctccatt tggggtcgcc atcaagttta aaacgctgta catctcctgc cactaaacca
    29581 agtccagcct tttgcccttc ttcgctacgt tctaacataa gctgagcacc taagcaaatt
    29641 ccaagaattg gcgttttatc cttcaaggct ttttcccgaa tcaactccac taagttcagc
    29701 ttctccaact gttccattcc actatcaaat gagcctacac cgggtaaaat aatatgatcg
    29761 gcatctctga tttttgcagg atcacgagta atttcactct tgcgacctat ctttttaagc
    29821 atattggaaa cggaaccaat attgcccata tcgtaatcga ctattgctat catggtgtga
    29881 ttaagcctca aatgtagcaa attcaggatt tataagtcgg aattgctaat attgcctcaa
    29941 agggaacaca cgtgaaaagg ccttatctgt atatacttgt aaccgactac ccatacggga
    30001 ttggcgagcc ttttttggaa aatgaactgc ttgaaaatag tgctcacttt gagcatattt
    30061 atctcgttat tccggaaatt cacttgattg atcaatctat tgttcgatat agccttcctt
    30121 ccaatgcgac tttagttccc ttacaaactc gggttagttt aacccataaa ctaaaggcaa
    30181 tgtcgcagtg gttgaagcaa tcaatccgaa aagaaattgc ttacataaat ggttcgtata
    30241 aattaaaatt cgggctgaac cattttaaaa ccattactgg atttatggct tctggaatag
    30301 ccttttcgga gcaatttcaa caagttctaa ggcaacataa tcatccgcca gaaaaagcaa
    30361 gcatttatag ttactggttc acctttgcta cttggggttt aacgctaatt aaacagggaa
    30421 attctgctta taatatcgga accagaattc atggctggga ttgtttttat tatcggaatt
    30481 cggcgaatta tcttccgctc cgccccatta cgatttcaac attagattgg atttgcccta
    30541 tttcagaaac aggaaaaacc catttaattg agaagatacc agatgttcaa ttaaataaaa
    30601 tcaaggttca ctatttaggg gtggatattc gtcctagtga atttccaatc tgctacaaac
    30661 aaggagtttt aagaattgta tcggtttctt ttatacatca tgttaagcgc atccatctaa
    30721 taattgatgc attagctaaa atcgaatcga ttcaggttga atggactcat ataggaagtt
    30781 ggtcttctca aactgcttgg ttacaagatg cggcgagaga aaaactagga ggaaaagaga
    30841 acattcaata tacattcaca ggtgaaaaaa ccattgaaga ggttcgttcc tttttagaac
    30901 atcatcaagc cgattttctt atttgcacta gtgaatcaga aggaatccca gtttccatga
    30961 tggaagcttt agctgcagga actcccgtta tttcaactgc agttggtggt gttcccgaga
    31021 ttgttcaaga tcaagaaact ggttatttac ttcctgccaa tccaacagaa gccgaaattg
    31081 ctgatacttt gaacaaaatc gtccttatgg atgaattgga ataccgccgt ttgtcggaaa
    31141 atgcaaaaaa tacatacctc aataagtttc aagctaaggt gaattataag aaatttgcca
    31201 aagaaatatt gcaggcttaa tcaatagcct aattcacact tacagatgca tatctgacta
    31261 tggaatataa aatttgctcg cgttgtatta tcgatactcc cacgtatcca gatatagtat
    31321 ttgatgaaaa tggggtgtgc aatgtgtgtc acacatatga tcagatggcg gagaaattcc
    31381 gaatcgaaca agatgttcta ggtgaaaatt acctgtttca gaaaattgct cgaatcaagg
    31441 aagaaggaaa aaacaaagaa tacgactgca tattaggaat tagtggagga gttgacagct
    31501 cctaccttgc tatcaaatta aaagaatggg gtttacgtcc actcgcactt catattgata
    31561 acggttggaa taccgaatta gctgtttcaa acattgaaaa actaatcaag caactcggct
    31621 tagatttagt aacctatgtg gtgaattggg aagaactacg cgatttggaa ttagcctaca
    31681 tggcggcttc agtagttgat attgacattc caaatgagat gtgcagcttt gctatcatca
    31741 atcggaaagc tgctgaatta ggacttaaat acatcattac cggtcataat tactcgagcg
    31801 aaggttggct gccacctact ttttcgcact acaaatacga tacactcaac ctaaacgata
    31861 ttcataaaaa gtttgggaaa gtgaagctca aaacatatcc taaaattggg tatttcagaa
    31921 cactctatta ttccaagatt aagaaaatcg acttctttag tccgcttaat tacatgcact
    31981 atgacaaaga ggaagcaaag aaaatcttga tcgatcagta taactggcgt gattacggag
    32041 gaaagcactt cgaaaattct tacacccgtt tttaccaatc gtacattctt cctgagaagt
    32101 tcaaattaga taaacgtcga tcacatttgt cttcacttat ttgtgctgga caactaacac
    32161 gagaagaggc actcaaaata attgaaacac ccaattattc aagtgaagaa ttgctagcat
    32221 ccgatcgtga ttatttcatt aagaaattga gaatcaccga gaaggaattc gaaaactaca
    32281 tccacacacc tcctaagaaa catacagatt atcgctctta cataaatgtc caagcattcg
    32341 tgaagaaaca tttttcattt cttaagaagt ttattccggc tccatgatat ccagaataat
    32401 cgcgaagctg atttatatgc ttcaaaaaag gcttaaaccc gaaatgatcg ggggctttcc
    32461 ttatagtaaa agcaaagatg ttcggataag taaccacaca catatttcct atccgaaaaa
    32521 tctgactgtg ggtgaacaag tttttattgc tcattttagc tacatcgatt gtttacagcc
    32581 tattacattg ggtcgtaatg tatacattgg aagttatgtg agtatcctta ctcattcggc
    32641 tcacaattct gttcgcttgc atggaaagca ttacctgaac gatgctccag atctagttgc
    32701 tttacaaaca ggtccggtta caataggaga ttactgctct attggacctt acacgatcat
    32761 tatgccgggc accacattag gaaaaggtac tatggtaaca gctaattcct atgtaaatcc
    32821 gggagtttat cctgatttcg caatcttaaa aggcaatcca gctaaaatcg ttgggaacac
    32881 gcgcgattac gatgaattat tgctcgaacg gaatcctgaa atgaggagca aatactattt
    32941 gaacgattat gattgaaatc cttcagtcaa atcaattatc cgatacatcg aatcttccgc
    33001 tttttttcac caaggaatgg cttagctatt caattgcaga aggcggtgag tttttttatt
    33061 tcaagttttc ggatggcaca cttatcccgt ttgtaacacg aaaacgctac ttcacgtgta
    33121 ttggctttct aatctataag ccctatcgaa tcggaaagtc cttgagtgct gatgaagaat
    33181 cttcttgtat tcaagagatg attgcctaca tcaaaaagca caaaatctgc gatgttattc
    33241 ttcctcccat gcacgtgtgc gtgttgcaga aagaagtacc taatgcctcc aactacaagc
    33301 tcgggttgat tttggtggat attaataaat cgcaagagga tgtgtttgcc ctgttctcaa
    33361 aaacctatcg aactcaaatt cgaggctgtg aaagagatgg atttacaatc agtcattctc
    33421 tagagcattt tgatttcttc tttcggatgt accgcatcaa ccaagagaaa aacaagaaat
    33481 atcccggtca agcatcccac atcaaaggca tacttgataa tatgcccaat caagcaaaac
    33541 tgtttactgt tctcaatccc gaaggtgagc ctgaaggttc aattttgttg atttttgacc
    33601 gtgaaaaagc ttactatttg atgggggcga aacacgaaga aaaacccact cataatggtt
    33661 cacagaaatt cctccaatgg agtgtgatgc gatactgtca agaaatgggt tgtgctcaat
    33721 acaatcttgg tggttttcgc tataacttgg atgaacaaga caaattcagc aaaatccaag
    33781 atttcaaatt aaagtttgga gctcttatcg aagaaggatt tcattttagt ttgaagatat
    33841 cttggaaata tagtctgtac gagaaactaa tagccttgaa acaaaaaatt aaatgaatcc
    33901 atcgctttta atcacattcg actacgaggt gtttctcggt tccaaaagcg gtacgattca
    33961 aaattgcttg atcgatccta ccaatcgggt tttaactatt ctcgctaaac acaattttaa
    34021 atcgcttttt tttgtagatg ccctctattt gtgccgttta caggaaagct cctcacctca
    34081 aaatcaagcc gacctaaaaa caattttcct tcaattagaa gatatcgtta aacaaggtca
    34141 tcaaattggt gtgcatatcc atcctcattg gctagatgct gaatatcaag aggattctaa
    34201 caactggaat ggcacgaaca agaatcactt tgccttgtcg tgtttagatg attctcttcg
    34261 cgctgaaaca attcagaaat cggtcgaaat attaagtccc tatatggaca tgactcgtcc
    34321 aattacctat cgtgcaggag gtttttattg tcagccattt agtttgctca aagacgcttt
    34381 tgcgcaaaat aatatttgga ttgatttcag tgtgatgcgt gattttacaa gtaccggata
    34441 taaaaatctt tttgctttcg atttcaccaa tcctccgcaa gaattaattt atcgatttga
    34501 tgacaatccg cttgtaccta ataaagcagg taaattcatt gaggtttcgg taaatagttt
    34561 tcgcttaggt ggattgtcaa gaattcgaaa ttctatttac ttccgattga atcgtactag
    34621 tccaaagatg aatcgaatgg gagatggctc gggctcgggg aattttatcg cacatcaagg
    34681 tgatgtagat tacagccgtt attttagatc gcatcagaat tatgcggtcg aattactaaa
    34741 ccctgttttg gctcatagtt acaatcagca actcaaggaa caaaagctaa ttcacttggt
    34801 ttcacaccca aaatctttta ctcctattgg attagaaggc tttgatttat tctgccaatt
    34861 agctgcgaaa caaaaacccg aaaccgatta ccgaactatt atccggaact acttaaatga
    34921 accacagtta aatctgtagc tgtttaaaaa agttaagcca gagtcattgg tttaattcaa
    34981 gatttctcaa gtagtttttc ctatttttcg ccaattaatt ccacaaaaac tcagttatgc
    35041 tttcattttc agagctgaaa cgcaatctca aaaaagacac ctcatctttt aagaaattgg
    35101 aaattgcaat cctgggagat tcatccactc aattcttaac tcaggctatc aaaggttatg
    35161 gcattgagca taaattgaat ctttctatat acgacgcacc atacaatcag ctcgaaagac
    35221 aactctccaa taaagcttcc gaattatata cacgcaaacc gtctttcata tttctatttc
    35281 tttctcctca aaaaataatt tcttcctttt actcgctttc taaatccgaa caagcatcct
    35341 ttgccgacga ttatgtgagt cgcctagaac aacatataaa tattgccaat agtgagttat
    35401 cggcgaattt catcatcaca aatattcctg aaatcaatga tcaggttttt ggaaattatg
    35461 gaaacaaaaa tccgtattca cttatttttc aaatcagaaa attgaattac gaattgatgc
    35521 gattatgcag cagttccgat aatttattca tcaacgatgt agcttcttta tcgaatcgtt
    35581 atggatatga ccaagcattt tcatcttccc tttacatcaa tggagatatc atttatcacc
    35641 tggatttcct accgacatta gcaaaaaact cagtcgatat tttacgtgca ttaagtggct
    35701 cagtaattaa atgtgttgtg ctggatctcg acaataccat gtggggagga ataattggag
    35761 atgatggaat tgacaatatt caattaggag aattagggat tggtaaagca tttactgagt
    35821 ttcaacgttg gataaagcaa ttaaaaaaca gaggaattct tttagcggtt tgcagtaaaa
    35881 acaacgatag tacagcacgt gaggtgtttg aaaagcatcc cgacatggtg ctaagattag
    35941 aagacatcac catttttatc gctaactggg aaaacaaagc tgccaacatt agtgcgattc
    36001 gagaaaactt gaatatcggt ttcgattcga tggtattctt agacgatagt gcatttgaac
    36061 ggaatttagt tcgcgaactt attcccgata ttatcgttcc cgaactcccc gaagatcctt
    36121 ctgaatacct agcatatttg caacatttga atttgtttga gtcgatttcg tttactgccg
    36181 aagatgaaaa cagaactcaa atgtatcggg aggattttaa tcgtactgcg ttgaaaaaag
    36241 tattcctcga cgaagacgat tatttgcgaa atctcgaatt gaagatgaat gttgaaacgg
    36301 ttaatgattt caatcttcct cgaattgccc aacttattca acgctcgaat caatttaatt
    36361 tccggaccat gcgctatagt ccggaagaaa tcaaaactat ttctactagt ccaaccgccc
    36421 gttgttattc gttttcactt gaagataaac atgggaaata cggattaatt tctaccatca
    36481 tccttcgttt agataaaaca caaggaactg catttattga ttcttgggtg atgagttgcc
    36541 gagttctaaa ccgtggtgta gaggatttca tccttaattt tatgatggaa tgccttgaac
    36601 aagaaggttt aaatacactt cagggagaat tcatcccaac agcaaaaaac agtatagtca
    36661 aagaattata cgcccaaaaa ggatttcagc cagatggtaa cctgtggatt cgcaaattga
    36721 gtgactatca accaatcacc catttcatcg cacgcaccga aactgttgct tagctttaga
    36781 atttctcatg cgctgtatag catagcagta ctgcattgga attaccaatg tgtattacat
    36841 atgctgagaa aatattaatt aagcttagct aaaatagctt cgcaaatttg tccaactgtt
    36901 ctgaatgact caatctcgtt ggatttaaag cggatgctat atttcttttc taccgcaacc
    36961 acaaattgca ggtgatttaa ggaatcccaa ttttctagat cgttcgtaga agtggcaagg
    37021 gtgattttat ctccatcttc ttccattatt tcagtaaaaa tgggtttcaa ttcaacgagt
    37081 agatcttctt gggtcatggc aaaattttaa aaaacaaaga taggtaaaat gtaaaatcag
    37141 ctaagcttga atgccaagct gtatttctct aaaggcaaac acctaaaatc ggaaaaagta
    37201 attcgtcgaa atacatattc aattgtatcc atcgaaaagc ttcaaattac acatgcttct
    37261 ataggattct acaaagacat ataacgttga tttacaaagc aattccgaaa ttcccaaagc
    37321 ggcaaattgt tcaattattc aaagagttta gcactaaaac acatcattaa gatttaataa
    37381 attatcttcg cggagttctg caagaagtgt aaaggatttc aacctttaac tagcttgtat
    37441 ttgtaggtta aacagcagat tttcatcacg catgaacttt attcagcaag ttattgattc
    37501 tagtaggttg ctaaatgtac tctacgacaa tctggataaa gaaataaccg gcaacccaaa
    37561 ctatcaaaag ctctgcgatt atctaagtaa ttacctagaa ttcaataagc ttagcattga
    37621 agaagccgtg gagatttaca cacgttttat ctcttcctac aacaaagatt gtcgccaatt
    37681 cattaaaaca aataaatatc catacgagct taaccctgaa ttagcaacac tttcacgaga
    37741 agaatacgac ataattctac ttttttccgt gttatttagt acccatcgtt ttcgaattat
    37801 ggagctgatt caagaaaaaa aaggttctgg aaaagctcta tatatcggac ttggtcccgg
    37861 actggaattg ctttacacac aagccgattt tacggagaaa attgcttacg atttagctgt
    37921 taacccattc ttaaacaagc agtttcctga ggttaaatta aatcacgagt tatatagcgg
    37981 acaaaatcct gattacttcg actccattta tatgatcgaa ttattagaac atgttccaga
    38041 tccattccaa ctgcttaaaa ttgcttacgc ttcgctaaaa acaggcggaa aagtactttt
    38101 cactacagct tcgaatattc cacaattcga tcacgtcttt aacttttcgc ccgaccacac
    38161 cgaatttgaa agcaaactcc aagaaatggg attcaacatc ctgtttaaag agaaaattca
    38221 acataactac ataactgtag acctgaattc gtcaaaccac ttttacgtaa ttgaaaaaac
    38281 gaaatgagca aaaaacaaga aaaagcattt caggaagagc gaagcttgat tattgacatt
    38341 ctgagaaaaa ctcgttttca tcagtttgat ccagctgtgc atcataagca agtaatccct
    38401 tatgcaacct attctccatg ggaaggcgat gcagaatttc ttgaactttt ttcaactatc
    38461 gaaaacaaca cactagtcga tatttaccgt tgctatgagc tttgggattt agtaaaaaac
    38521 tctgctaata tcgaaggtga tattctcgaa gtaggtgtgt ggcgtggtgg aacaggagct
    38581 gtaatgtgca aagctgccga aaactcaaat agcaaagtat acttagccga cacattcgaa
    38641 ggcgttgtta aagctaccga agaagatacc gtttaccgtg gtggcgagca ttccgacaca
    38701 tccgatcaaa ttgtgaaaga cttgctagca aaggtaaacg tgaaaaatgc tcacatccta
    38761 aaaggcattt tcccggatga tttccccgaa gttcaaatcg acaaattgaa attgtgccac
    38821 atcgatgtgg atacctacca atcagctaaa gatattttcg actatgcttg gcctaaaatg
    38881 caagtcggag gagttgttgt gtttgatgat tatggcttct ggacttgtga aggtgtaacc
    38941 aaatatttca atgccttgga gttagctgat ggacgtaaaa tccacaacct caatggtcat
    39001 ggcatgatca tcaagtgcaa ataaatcacc gattcacatt cgccaacgaa attaatcccg
    39061 tcaacaccag ttcacgaaat ggctcatcaa aacacgcata cccattgtat tctgtgtaat
    39121 gctgaaaagc tcaaacctct agttggtttt gagaaagcct ttctaactcg ctgttcaaac
    39181 tgtggtttcg ttttttctgc acgcattcca acaaatcagg aacttatcac ctattacgaa
    39241 ggatacggac gaaacgatta cctcagtccg attaccatca aacgatacaa cgaaatctta
    39301 gatcagtttg aatcggttcg caaaaccaat cgattactgg atgtaggttg tggaattggc
    39361 tatttcctcg aagtagctaa aaaacgtggc tgggaagttt atggcaccga atataccgac
    39421 gaagccattc ggatttgcca atctaaaggc attcaaatga atcaaggcgt gctcgatcca
    39481 gctaattatc aacccgaaag cttcgatgtc atcacctctt tcgaagtaat cgaacacatc
    39541 aataatccgc aaagcgaaat tactaacatc agcagtttac taagaaaagg tggcttgttt
    39601 tacgttacaa cccccaattt caactccctg cttcgctacc acttaggcga aaaatacaat
    39661 gtcatttgct ggcccgaaca cctctcctat tacactcctt cgactctcaa tcgattgatg
    39721 caggcaaata acttgcataa actctacatt caaactaccg gcatcagtat tactcgtctc
    39781 attaccagcc gaaaacctgc tccagcacct gcagtagaaa gcaataataa cgaaggaacc
    39841 gaattcgtat ccgaaaatac ccaagacgaa aaactccgca accgctttga atccaatcct
    39901 ttactcggct tgacaaaaaa agcaatcaac ggcatgctca cctggacagg aaaaggcgat
    39961 gcattaaaag gattatttcg aaaagtataa gtcgaattgg gcggcagaac acgctttacg
    40021 cctatatcct tcttcgcaag ctcatcaggg atgccggctt caatcgttgc cgctcacagc
    40081 taattgtagc tatcgttttt cctatatcga tcttctaatt ctatccattt ttcccgttct
    40141 tttgtcccaa tttcgtactt aagaatttcc aattcgtaat tcgtaattcg taattcgtaa
    40201 ttcgtaattg ataattcgta attctaaaaa cttcaccgtg caacaactta tcgaatgcgt
    40261 tcctaatttc tccgaaggga acaatctcca aatcatcaaa caaattaccg acgaaattga
    40321 agccatcgaa ggcgttcgtt tactcaacgt agatccggga aaagcaacca atcgtacagt
    40381 tgtcacattt gttggaacac ctgcacaggt tatcgaagct gcttttcaag ctatcaagaa
    40441 agcttccgag cttatcgata tgagcaaaca taccggcgaa catcctcgaa tgggtgcaac
    40501 cgatgtttgt ccgcttattc ctatctcggg aattagcatg gaagaaaccg ccgaatatgc
    40561 ccgtaaactc ggcaaaaggg tgggagaaga attaaacatc ccagtttatc tttacgaatc
    40621 ggctgctgtt attcccggac gaaacaacct cagcgaaatt cgcgcaggtg agtatgaagg
    40681 ttttttcaag aaaatcacgc aacccgaatg gaaacccgat tttggtcctg ccgtaatgaa
    40741 cgaacgtgct ggcgccagtg taattggtgc gcgcgatttc ctcattgcat ttaacgtcaa
    40801 cctcaacacc acttcggtaa gaagagccaa ttccattgct ttcgatgtac gtgaacgtgg
    40861 ccgtgtaaaa cgagaaggtg atccagttac cggtaaaatc gtaaaagacg aacagggcaa
    40921 tcaagtccac atcccgggaa gcttaaaatc agtaaaagct atcggttggt ttatcgaaga
    40981 atatggcgtg gctcaaattt ccatgaacct caccaacatt cgtgtaacac cagtgcatat
    41041 tgcattcgac gaagtttgca aaaaagccga ttctcgtgga attcgtgtta ccggttctga
    41101 gttagttggt ttgttgcctc taagttgttt gctagacgcg ggaagatatt tcctcgaaaa
    41161 acaacaacgc tctacgggtg tttccgaagc agaactcatc aaaattgctg taaaatcaat
    41221 gggtctcgat gaacttggtc catttgaacc cgaaaaacgc gtgatcgaat acctattacg
    41281 cgatgcatcc gaagagaaat tagttagcat gagtttgaaa gcttttgccg atgaaacttc
    41341 atccgaatct ccagctccgg gtggcggttc aatctcagct tacgtgggag ctttaggaat
    41401 tgcattagca ggcatggttg ctaatcttag ttcacataaa cgcggctggg atgtacgatg
    41461 gaaagagttt tctgatgtag cagatcaagc cgaagaactt aaaaaacaac ttatccgcat
    41521 ggtcgacgag gacactcgag ccttcaaccg tatcatggat gcatttggat tggcaaatgg
    41581 aagtcccgaa gaaaaagcag cccgtaaagc agccattcaa gaagcaacac gatacgcgat
    41641 tgaagttcca tataaagtaa tgcaactcag tgcttcaaca cttactatca tccaccaaat
    41701 ggccgatcag ggaaatccaa attcagtttc tgatgctggt gtgggtgctt tatgtgctcg
    41761 aactgccgta atgggagctt ggctaaacgt aaaaattaac tgtgcgggat acgaggataa
    41821 agcatttgta gatcaaactt taaacacctg cgaatccatt gtacaaggag ttaatcaaaa
    41881 agaagcgagc atactacaat tggtacatca gaaaattgcc aatggttaat ccttgtctaa
    41941 gaaaacagaa agcccggtat taaatcgggc ttttttatgc acttaatcga tcaaagtgac
    42001 tcgaccaatc atttgtttag caatatgttc tgtgttggta atagtagccc tccacacata
    42061 aacatcttcc tgaactcggt tacccatata agttccatcc caaccttcaa gtggatcggt
    42121 tccttcataa ataagctctc cccaacgatc gaaaatctcc agtaagaact tcgttgcata
    42181 cgttacccga atcatgaaaa cctcgttgtt tccatctcca ttcggtgtaa aagcattcgg
    42241 agcgaaaatg gttgggaatg gatcaatctc taccatttta caaatttgat ccacacatcc
    42301 atgcgaagta gtaacacgca ggcatgctgt aaaatgccca gtatcaggaa acgtatgaag
    42361 aggtgattca agtgtagacg tttgtccgtc gccgaaagtc cataaatacg tactagctaa
    42421 agtactttcg ttgataaact gatactgtgg attctgtata tcagcattat cgggattgta
    42481 actaaatcgc gcatcaggca atccgtaaac agtaactgct ccgggagccg aaagtgtggc
    42541 agtacaacca aactcatctg ttaaatcaac cgaaacatct tgcaatccac tttctgtgaa
    42601 gcatattttt tgaatggcat tcacaccttg actaccattc gagaagctcc aattaaacga
    42661 ttgaatagct gccgaaccag tcgagttcac ttcaaaattc acacagatag ggctacaacc
    42721 ttcggccaca tcagttgtga tgtttccttg aggacgaaga cgtaatgtaa cttgctgtga
    42781 atcggtagca gcacatccaa ggacatccgt ggcaacagct gaatacgttc gtgttgcagt
    42841 tggtgtatct actaatgtat caccttgaaa attgaatgct ggccaagtta cttctatgtg
    42901 gctagttcct cccgaagctt gagcaattag atgagcttca tcgttttcac acacatacga
    42961 aggacctgta acatctataa caacaggatc gggctgagtg agaactatgg atgaagtttg
    43021 aacacaccca tttccatccg taacacttac ctgatatgtt ccagctggta tgctgtcgag
    43081 cgaagctcca tttggtccat ttagccaagt gatggcataa ggcaaagtac ctgcttgtac
    43141 atcagcttga agggctccat cactaaatcc aaagcatgta attggatgtg taataaatgg
    43201 tgcaatttga acattcggaa tagctgctat agtcatgacc gaatctaacg tacaaccatt
    43261 cgcatcgcgt acttgaacag aatagaaatc aggcgctaga ttagtataaa ttacctgtga
    43321 tccaaaagca ccaccattaa aactatattg atatgcgggc gaaccaccat tcacttgtag
    43381 aatcgctagt tgtccattgg ctaaatcaca cgttgacgaa tcgactgtaa tctgcatttg
    43441 atctggccct ggaaattgtt gtaatacaac taccgaatca atcatacaac cattcgcatc
    43501 ccgaactatt aaaggataat tccccgcatc caaatcagag cggattactg gatagtcggt
    43561 tatcgtagtg aaactggagt caatccattg aacttggaat ggagcaatac caccttgaac
    43621 acccatcaaa cgggcattac cgttagcttg agaacaaaca gcatcctgac tttgcagcgt
    43681 catgaatgtt ggatacgaag gcatggctaa ttgagcttgt cgagcagatg tacatccatg
    43741 agcatcaaca atttgcagag tataagaccc aacatctaat tgagtaaaca tatccgcttg
    43801 ttgaagagct ccgccattca gttgatattg ataaggtgtt gtaccaccaa aagcagtatc
    43861 cgcttgaatt ataccattgc taacagcaca atgagggttc tgatagctta tttgtaaagt
    43921 caataaagct ggttgagtga taactacttg agcagtatct cgacaattta atgcatccgt
    43981 aacataaaca tcccatgttc ctgcagctaa actacttgcc agagaatctg tttcactgtt
    44041 agtccacaga aaagtaaatg gtgctgtacc cgcttgtatt gttgcaacag cctgtccatc
    44101 ggatccatta aaacattgag cattttgtat cacctgagca gtaagtgcta catcaggaat
    44161 aagcgtaata tctatgagcg tatctaatac acattgattt tgatctcgca caataagcgt
    44221 tacgattccg atagcttgtt gttgataggt tgtttggtta ctaaatggac ttccgttaaa
    44281 actgtattca aaagctggag ttccaccgtt aatcgttcct aatgttacaa tggcattgtt
    44341 taatccgcaa ctagctggct gcacagtata ctgaagtgat ccgggaccat ttatttgctg
    44401 taaggtgacg ttagtatcta ttatacatcc attaacatcg gtcaactcca cattaaaagg
    44461 tccggcaggt aacgaatcga atgtaacggg aacgggtacg actcccatat cagagccatt
    44521 cacccaaatt tgaaaaggtc caatcccacc aattacttga agtggagcaa caacaccatt
    44581 gtcttcacca caagttgcat cagtaagact catcataagc tgagtaggat aactcaacat
    44641 agtaatggag ccatttactt ggaataaaca tccattcgca tctcttaccg aaattgtatg
    44701 cgttcctata gctagtgaat taaattgtga gagtgtcgaa taaactccac catctatcgc
    44761 atattcataa ggagaagtac caccagttgg agcattaacg gttattgttc cattattctg
    44821 gttacacgtt gcgggttgaa tagatagtgt tgttgttatt ggagcaggtt cggatattgt
    44881 gaaactagta tcagccaaac acccaaaatt atctaccaaa tgaactacat atattcctgc
    44941 tgataggcca gtatcaatag ctgtagcatt tccatcatcc catccataac t
//
LOCUS       DAOOQW010000003        40379 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_1113_length_40379_cov_5.740899, whole genome shotgun sequence.
ACCESSION   DAOOQW010000003 DAOOQW010000000
VERSION     DAOOQW010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 40379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 40379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40379
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1113_length_40379_cov_5.740899"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2623
                     /gene="uvrA"
                     /locus_tag="PK281_00465"
     CDS             <1..2623
                     /gene="uvrA"
                     /locus_tag="PK281_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011709665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOY47836.1"
                     /translation="SSYARQFLGRINKPDVDFIQGISPAVAIEQKVNSTNPRSTVGTS
                     TEIYDYMKLLFARIGKTISPVSGEQVFRSTPADVVDFINTQPKGAKVSIYAPLNNDRF
                     DNKPANLSLLRQLGFSRMRIGQELKRIEDWENDGKTLKGKTLELLIDRFVVNQEDEDN
                     NSRISDSVQTTFYEGNNECIVEVVLEDGTILTKRMNNRFEADGIVFEEPSVHLFSFNN
                     PFGACKTCEGFGTVIGIDEGLVVPDKSLSVYEGCVAPWKGEKMSEWRDNFVKYAMKVD
                     FPVHRAYYDLDENQLDQLWNGTPHAEGINAFFHYVEEQAYKIQYRVMLSRYRGKTSCP
                     DCKGTRLRKDAGYVQVGGKSIIELVLMSIDRVKAFFDQVELDEYQAQVAKRVLVEIQS
                     RLGFLCDVGLGYLTLNRLSSTLSGGESQRINLATSIGSALEGSLYVLDEPSIGLHSRD
                     TDRLITILKRLRDLGNTVIVVEHDEEIMKAADVLIDMGPEAGTHGGNVVFQGVHADLI
                     TAGSSLTADYLTGRKEIALPAKSRKWNQSIQIKGARHNNLKSVDATFPLGVMTVISGV
                     SGSGKSSLVNKILYPALKKMFEGYSEQTGQFDRLEGDYRSIGAVEYIDQNPIGKSSRS
                     NPVTYVKAYDEIRTLYSIQPVAKTRGYKPSAFSFNVEGGRCEECEGEGFVTISMQFMA
                     DIKLQCDVCNGKRFKDEILEVEYNGKNIYNILEMTVEDAINFFSNGKNVATDQRIVAK
                     LQPLADVGLGYVHLGQASSTLSGGEAQRIKLASFLTKGSSAVHTLFIFDEPTTGLHFH
                     DIRKLLDAFNALIAQGHSLVIIEHNLDVIKCADYVIDLGPEGGDTGGQVVFEGTPKDL
                     AKREDLWTGRYLKEKFS"
     gene            2682..4055
                     /gene="hisS"
                     /locus_tag="PK281_00470"
     CDS             2682..4055
                     /gene="hisS"
                     /locus_tag="PK281_00470"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019388299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HOY47837.1"
                     /translation="MKPSLPKGTRDFSPLETARRRYIFETIRESFELYGYQSLETPAM
                     ENLSTLTGKYGEEGDRLIFKILNSGDFLKEVREQQSDLEAVNKVLPLISEKALRYDLT
                     VPFARYVAMNKDKLAFPFRRYQMQPVWRADRPQKGRYREFWQCDADVVGSASLINEAE
                     LIQIYDRVFSAFRLPVKILINNRKILSGLAEVIGEPERLIDITVAIDKLDKIGIEKVE
                     QEMRDKGVSELAIEKLKPLFTISGGIQEKTSFLRDFLSSSETGKKGIGELETLLNYFQ
                     GEDGFKLETSSLELDLTLARGLNYYTGTILEVKSKDVEFGSIGGGGRYDDLTGIFGLP
                     GVPGVGISFGADRIYDVLLMQDLFPASAITGTEVLIANFTKEDERAAIPVLQLLRGEG
                     IKAEIYPEISKLKKQFEYAEKKGIPFLMIYGEGERADNCILTKELSSGKQISVPLEKL
                     SFFRELF"
     gene            4115..5614
                     /gene="hutH"
                     /locus_tag="PK281_00475"
     CDS             4115..5614
                     /gene="hutH"
                     /locus_tag="PK281_00475"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020214224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HOY47838.1"
                     /translation="MSGQAIDPSHCYLERIHHLLKSGERIFLSDEAKASIQKGRNYLD
                     ERLAKSDAPIYGINTGFGSLCDVQISHDQLEQLQENLVKSHACGQGEPVPPAIVKAML
                     LLKVQSLTYGLSGVQEITVQRLIDFYNENVLPVVYQQGSLGASGDLAPLAHLCLPLLG
                     LGEVWVNGIRQPAASVLERFGWEPIKLQSKEGLALLNGTQFMNAYGVSLSAEAMRLFN
                     WATVIACISIDAFDGRPDPFHPRIHEIRHHHGQQVIAATVRRVLEGSEILNQDKVHVQ
                     DPYSFRCIPQVLGASFDVLRHAESVFTNELNAVTDNPTIFPDEDLIISGGNFHGQPLA
                     MALDYLCLAMHEVGSISERRTYQLIGGKRSLPPFLIAKPGLNSGLMIPQYTQASIVSQ
                     NKHLCMPASADTIDSSNGQEDHVSMGANAATKAWRVMENLEKILAIELLNAAQAIEFR
                     RPKKSSPIIEDLLVAYRKVVPFVDEDRLLYTDIENSRQFLKNHHLPLFI"
     gene            complement(5593..6243)
                     /locus_tag="PK281_00480"
     CDS             complement(5593..6243)
                     /locus_tag="PK281_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOY47839.1"
                     /translation="MKKVWNPLYIGVISYFCLVLPAVILWGINFRKLGKPNLTWLTFL
                     VGGLLFLLLVLGWIFLPQSWDWALEVFHIILSIAGAAFQYSYFHKFLEADEENHQRES
                     LIKPALLSILFVLTVVSLFIGWNWYLQHQTEQELLLAKEKYDIGDYPASVKILNSIIE
                     EDPGQRTAYINLSIAYETMGKSDSAIYVLDNWLIQMPEDSEVKDRIYQLRYMNSGR"
     gene            complement(6257..7291)
                     /locus_tag="PK281_00485"
     CDS             complement(6257..7291)
                     /locus_tag="PK281_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY47840.1"
                     /translation="MRKLLLLIGVLMFVVSCKKDDPEPTPIVPVEVSKTALILNEGNF
                     QWGNASLTRIDLKSGTVSADVFQQANQRPLGDVFQSLAMVGDELWLVVNNSQKIERVN
                     KNTFVATSPVTGIQSPRYLLPYSNRVYVSDLYANTVHVINSTSGTLMSEIPFPGWSEG
                     MIKDPQNLIWISNVTRGKLMLLNPETNLFVDSITVGDAPKDLIMDKNHRIWVLCEGHI
                     PPAVETGGSLWCIDPVSRSVLKSFQLSTLDHPSKLNSILDASSLIYLNKGVFKLDVNA
                     LELPTSPFIAQGSRLFYGLGYNAIDSTIWASDARDYQQAGEALVYKAISGEHLRSWTV
                     GIIPGAFYFY"
     gene            complement(7288..8283)
                     /locus_tag="PK281_00490"
     CDS             complement(7288..8283)
                     /locus_tag="PK281_00490"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY47841.1"
                     /translation="MRTILFFLLFAIPLLTKAQFDPPAGQPGSLAIHVSSISFVAWAN
                     SAVVQRSWMQMGVDSLGYVSSGIEAYALGKAGENPVVSLGDGGSATLQFAHSIRNGAG
                     PDFAVFENSFDGSFLELGFVEVSSDGENFFRFPAASLTDTSQQIAGFSYIDAKNLNNL
                     AGKYVMLYGTPFDLQELDSISGLDINSITHVRIVDVVGSILPQFCTRDSEGRMVNDPF
                     PTPFMSSGFDLDAVGVIHNNDPAAIDFSTHSEPPIVLYPQPASTFLQVKCDKSISGNW
                     MASVYSSDGRTCSNQAIEKQDDGFSIDVHSLKAGIYILLLSNNQQVIRKRFIIQK"
     gene            complement(8283..9263)
                     /locus_tag="PK281_00495"
     CDS             complement(8283..9263)
                     /locus_tag="PK281_00495"
                     /inference="COORDINATES: protein motif:HMM:NF026067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4465 domain-containing protein"
                     /protein_id="HOY47842.1"
                     /translation="MKIIFLTTLIFISNMIGFTQTTVDFEDLTLSPDSWWVGTDGVST
                     GFTSHNAFFPTRWDTAFGGYWLDGFAYSNMTDSVTSGYLNMYSCKAASGNDGSANYAI
                     AYDDGRFVPGNANGLYAFEPIELYITNNTFAFNNMRDGDGFSKKFGGVSGNDPDFFKV
                     TFTGIRNGEATGTDVVVHLADFTFEDNSLDYIQREWLRVDLTAMGVVDSIVFSFESSD
                     VGEFGINTPKYFCLDDFKVNVVPNSINTIAKSTLSVFPNPVAEILSIRQVNPERIDYS
                     IYSSSGMLVKNGSSNSVTEHVNVSELASGLYLIHLHADGVADTRTFVVQH"
     gene            complement(9260..11176)
                     /locus_tag="PK281_00500"
     CDS             complement(9260..11176)
                     /locus_tag="PK281_00500"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOY47843.1"
                     /translation="MKNRIFTCIFFLLIGLRSIGQTAAKDSVHQLPAFQVQDNRIPSS
                     NPGLQQSVFSTQQAGKNIQSLAELLSRNSASFIRQYSPGTLSSPSIRGTGAAHTALVW
                     NGLNLQSSMNGQMDLSLVPAILFDHFSLQSGAGAASWGTGAIGGTIHLGSEHSNNHLL
                     IQQSIGSFGFKQTAVDVAYTLGRISFRTRLFQSQVENDFHFINNAVFGHPKQKQLNAA
                     QRMKGWMQDLYWVTGKYSQLQLSVWGQTSIRQIPPIETIPIAAASQQDETWRGMLSWK
                     RVSSKFDIQLKTGYLQEGILFNDSLLSLHAANHAKTYLAETEFGKNFPSIHSRFQLGG
                     NFSYSSAHAQGYGSSAVHQERRAGFTSFRSELFKGKLVTLIQGRQEWFNRQFVPFIPS
                     ISASYPLFKTLTIRGQVARSFRVPTLNDLYWQPGGNLSLRPESGIASEFGLNYYVEKL
                     PFVLLGKASVFSNQVKNWIVWLPSNSFWTPENVPQVHSRGLDAGIEIHRHFKKKQSLH
                     FFSNAQYVWVTAEKSDIGSIVESGKQLVYVPHFTCNARMSYRWKGFQFELSSTYTGER
                     FITSDNEKSLPGFFLLNAFVSKQLELKEHHFSFFLNAFNLLQKSYQVIAWRPMPLLSF
                     QTGISFTFQPNPTK"
     regulatory      complement(11282..11463)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            11573..14422
                     /locus_tag="PK281_00505"
     CDS             11573..14422
                     /locus_tag="PK281_00505"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOY47844.1"
                     /translation="MKNTLMLKRISFLSGALSLMLLLPATAQKLVEKVEKKGDEIVIP
                     YEKYKLDNGMTIIIHVDKSDPIVHTDVTYHVGSAREQIGRSGFAHFFEHMMFQGSDNV
                     ADEEHFAIVTEAGGTMNGTTNLDRTNYFETLPSNQLETSLWLEADRMGFLLDAVTQQK
                     FEIQRATVKNERGQNYDNRPYGLVGERTNEALYPYGHPYSWPTIGYLKDLDRVTVDDL
                     KRFFLRWYGPNNAVLTVAGNVDPAEVVRLAQKYFGSIPRGPEVKPMDKMPVTLDKDRY
                     ISMEDNIRFPMIQMTWPAVSSGSDDELPLDLLAELIGGDKTSILYQNLIKTNLAVQAR
                     AYNPAAELAGTFVVNVLPFPGKTLADMEKIIRDAFVEFEKRGVKDEDLIRIKNSREAQ
                     MIQSLESVSGKASKLAYNETFKGNPNYIKKEIDQLQKLTKEDVIRVYEKYIKGKPAVI
                     TSVVPKGQKELIAHEDNFTPKRATEAESAEGSEYKNLSYVKAKDVFDRSKHPAPGANP
                     VIRLPEFWKETSANGLQIIGTQDNDLPIVSMLISFPAGHAAENPAKAGLSNLLTDVMS
                     ESTLKYSSEEITSELEKLGSEISISSDREYVYVNVFSLKKNLARTIELLEEKLFHPKF
                     DEEEFNTAKAQEIAAIDNNATRAATIADNVYSKLLYGDHIFGIPVIGTKASLEGITLQ
                     DVKDFYAAQIIPAKAKLVAVGQITSKELNTAVPFLLNWKASASPVEIKQPATPAIAAT
                     TIYFVNKDKAPQSEIRIGYMALPYDADGEYYRAGVMNYTLGGNFNSRINLMLREKKGY
                     TYGARTGFSGTLYRGSFTASAGVKAEKTDSSLMDFFSEMNAFRASGITPEELNFTKNS
                     LGQRDALKYETPFQKALFLQNLLVYNLDKNYIDKQSAILGSLTKNDIDGLAKRYLPTD
                     KMIVVVVGDKASNWDKIKALGYPVVELDTEGNIVK"
     gene            complement(14467..15273)
                     /gene="murI"
                     /locus_tag="PK281_00510"
     CDS             complement(14467..15273)
                     /gene="murI"
                     /locus_tag="PK281_00510"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HOY47845.1"
                     /translation="MDKNAAIGIFDSGIGGLTVAAAVHQLLPHESLIYFGDTAHFPYG
                     DKSPARIESYGLGIAEYLLSQGCKMIVIACNTASAHAFKAVTKFVGNKAIVVNVIDPA
                     SQVVAERYPNGRVGVIATRGTIQSGIYPRKIKKYSAHTEVTSLATPLLAPMIEEGFFN
                     NRISRTVIHSYLEKKSLQNIEAIILGCTHYPLIRKEIEEFYKGSVDVVDSAEMVALTV
                     KNLLAESDLLSTQNNPHLRFLVSDFTESFESSATYFFPEKVNLEEQDIWK"
     gene            complement(15333..15845)
                     /locus_tag="PK281_00515"
     CDS             complement(15333..15845)
                     /locus_tag="PK281_00515"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOY47846.1"
                     /translation="MKQLIALALFATALIGTPALAQKSNKFGHINSAELLSLMPERKT
                     AAAKMDSITKDVEKQLQEMMTEYRTKQEKYTNEAPKLSELVKKDKEEELTSLGTRIQN
                     FQQQAQQSLEQQEQALIEPIVSKAKKAIETVAKENGYTYIFDTSAGSLLFWEESDNIL
                     VMVKKKLNLP"
     gene            complement(15860..16459)
                     /locus_tag="PK281_00520"
     CDS             complement(15860..16459)
                     /locus_tag="PK281_00520"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOY47847.1"
                     /translation="MKKLILALLVFFAVSSSVSAQKFAYVDTQYILENLPEYKSAQQQ
                     LDRISIQWQKEIEVKFAEIDKMYKDFQAESILLTDDMKKKREEEIIDKEQAAKELQKQ
                     RFGKGGDLLKKRQDLIKPVQDKIYNAIKEIATTKNYAVVFDKSSDLTMLFTNPKYDIS
                     DQVLESLGFTPGQKGDDQDSNDKGGGSSPSSPTPPTNKK"
     gene            complement(16588..19119)
                     /gene="bamA"
                     /locus_tag="PK281_00525"
     CDS             complement(16588..19119)
                     /gene="bamA"
                     /locus_tag="PK281_00525"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HOY47848.1"
                     /translation="MTRLYFRLFLLFCFLTSQQIVFAQVIPDNNPIGISYSAPKEYEI
                     ADISFKTKDNSNLDQNILRLLSGLNKGDKIMIPGDRISSAIEALWKQGLFTGIEVKTA
                     KIEGDKISLIILLEERPRLSRFRFVGVKKNEADDLREQIKLIRGKPVNENLMVNTRNT
                     VREFFIDKGYMNVAVDVNTTNDTIPGGGVMMNILVNKNKRVKIHQININGNQFVTDRK
                     LKKKMKNTKENPWYSLFTTSKYMEDNYVEDKSKILAVYFDKGFKDARIVKDTVYRYDA
                     KHINIDIQIDEGRQYFFRNINWVGNVKYTSKQLSDILGIKKGEIYNQATLEQNLFMNA
                     SESDVSSLYMNDGHLFFQVTPVETFVDNDSVDIEMRIYEGKQARINRVTVTGNDKTND
                     HVVMREIRTRPGQLFRRSDIIRTQRELSQLGYFDPEKMGVNPVPNPQDGTVDIEYKVE
                     EKPSDQLQLSGGWGAGRLIGNLGLAFNNFSARRFFKKDAWRPLPSGDGQRLSLNASST
                     GRAFQSYNLSFTEPWLGGKKPNSLTITAYHSIYGNGAAKNDPTRSLTKNTGIILGYGR
                     RLRWPDDFFTLQQSVSFQYYELVNSTAFELANGFANNLSFTTTLSRNSIDGPIYPKSG
                     SNISLSIQFTPPYSVINNTPASELVTLEQRYKWVEFHKWKFDAQWFTPLSRNKNTLVL
                     MTRANFGFVGLYNQNKGLSPFERFRYGGTGLSGLTWGAQFLGAEIVGLRGYQDGSISK
                     GATGGVGDAPIYSRYTMELRYPISLNPSATVFALGFLEGGNAWTNFKDFNPFSIKRSA
                     GVGVRVFLPMFGLLGLDYGFRFDEVPGFSGMPKSQFHFIIGQNFN"
     gene            complement(19116..19880)
                     /locus_tag="PK281_00530"
     CDS             complement(19116..19880)
                     /locus_tag="PK281_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007095232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HOY47849.1"
                     /translation="MAQAKKVNKEDINLHTLPSHIAVIMDGNGRWAKMQGKLRTFGHE
                     NGVKAVRETVEACAELGISYLTMYAFSTENWNRPKYEINALMTLLVSTISKETATLMK
                     NNIRLESIGDIDSLPGKCRRELEEAKKKTAGNSRMTLVLALSYSSRWEITQAVKQITS
                     DVQIGKIKTEDISEKLIANYLNTSGMPDPELLIRTSGEHRISNFLLWQISYAELYFTD
                     KLWPDFRKEDLYAAIVSYQQRERRFGKTSEQLNTPS"
     gene            complement(19918..20754)
                     /locus_tag="PK281_00535"
     CDS             complement(19918..20754)
                     /locus_tag="PK281_00535"
                     /inference="COORDINATES: protein motif:HMM:NF039345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HOY47850.1"
                     /translation="MRKWIFLSLIGLATSFSANSQVFNRYSELGLFGGVSYYMGELNP
                     GKQFFMVRPGGGLVYRYNTSRRFAWEIHGLYGSVQAEDARSGEDNQILRNLSFRSQII
                     EFAGLLHFNFFNYEIGNPETPATPYIFGGLGLFRFNPKADFQGEWIALQPLGTEGQGS
                     PVYADRKQYSLMGANIPFGFGVKFHAFGRLGFSLEWGLRKTFTDYLDDVSTTYADPDV
                     LFATHGELAALLADRSLVNPGKINAGRQRGNSTNKDWYSFAGLTITYKIVPRKAECAA
                     YK"
     gene            complement(20788..21714)
                     /locus_tag="PK281_00540"
     CDS             complement(20788..21714)
                     /locus_tag="PK281_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY47851.1"
                     /translation="MRKLSILTVLLLFISISAQAQRKGKGRFLDRCESTYGIGFTNFL
                     GELGGADQFGTNGIKDLEMILTRPAVHLGFRYRKNPVLSFKFNLNYGRVAGRDSLTRE
                     EFRRNRNLSFRSPIFESSLQVELSIVQLKSSKSIYRLQGVRGKSGIDLNVYLFAGVGG
                     FYFNPKALLGGRWWALQPLGTEGQGIAASRAPYKRFQMCFPIGFGFRVPMQDKRWTIG
                     FEYGMRITTTDYLDDVSTTYFSPTLIADKNGPVAAALSNRANPFSPDFIKGSAATGQQ
                     RGDPTDRDAYMFGIVTMTYRLKSGKSILPKFR"
     gene            complement(21807..22685)
                     /locus_tag="PK281_00545"
     CDS             complement(21807..22685)
                     /locus_tag="PK281_00545"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF002521.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HOY47852.1"
                     /translation="MQIAVFGRSFTESRAPVIKELYFLLHQSGVQVQILEPFFKASQS
                     LMPIGANFSRFKNQDDLDNRTSCMITIGGDGTLLESVTLIRNTEIPLVGINTGRLGFL
                     TGYSQDEIAKAVEAALNQNYLLEKRSVLQLKMNGGPFADFPYALNDITLHKKESSTMI
                     TVHARLNGEHLNSYWADGLIVASPTGSTGYSLSCGGPILMPESPSFVITPIAPHNLNV
                     RPLVITDEHSLSIDVEVRGKNFLLALDSRSASIPAKTEITINKAPFSIRLARKPEQPF
                     LKTLREKLMWGIDRRN"
     gene            complement(22760..23422)
                     /locus_tag="PK281_00550"
     CDS             complement(22760..23422)
                     /locus_tag="PK281_00550"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOY47853.1"
                     /translation="MLAQDLISDTIPPLKTSDSGIKALHWMDEFRVAHLPIVNNSEFL
                     GLISDTDILDLNAPEEALGNHPLSLIRPFVYESEHIYEVLKLAARMHLTLIPVLDAEN
                     KYLGNISLRSLVEKFAATVSVQNPGGIIVLELNQNDYSLSQIARIVEENDTKILSLSV
                     SNNPDSTQIELTIKVNREDIRGILASFARYNITVKATFQQTEFSEDLRNRFDLFMNYI
                     NM"
     gene            23561..24283
                     /locus_tag="PK281_00555"
     CDS             23561..24283
                     /locus_tag="PK281_00555"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014938323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HOY47854.1"
                     /translation="MTLLSVNINKIALLRNSRGSNYPNVADTALNLERFGAQGITIHP
                     RPDQRHATYEDVFTLKPILKTEFNVEGYPSRTFLDVVLKAKPHQVTLVPDPPDALTSN
                     AGWDTIKEFSFLQEVVTELHQQGIRVSLFMDTNLDLYDQAAKTGTDRIELYTEAYARH
                     YSSAPEQAIEPYIKAALRAKQLGLGLNAGHDLNSQNLKYFAQHIPDLLEVSIGHALIS
                     EALYLGLENTVQIYLRQLHEAI"
     gene            24270..25040
                     /locus_tag="PK281_00560"
     CDS             24270..25040
                     /locus_tag="PK281_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOY47855.1"
                     /translation="MKLFERHFGTGETVVILHGLFGQSDNWTSIARKLGEKYCVYTFD
                     LRNHGQSGHSDEMNYRVMASDVLETIDGLGLDKIHLIGHSMGGKVAMLFGLTYPERLN
                     SLVIADIGPRYYKPHHSDIITGLSNIDLSSLKSRQEAENQVLDFIPDFGTRQFLLKNL
                     YRLGENQFAWRFNLPVISRTIENIGEAIDSGLCQIATLFYRGSTSSYIKPEDIPAILN
                     QFPHAQFEVMQDAGHWLHAEKPVEFIETIENWISSQST"
     gene            25221..25583
                     /locus_tag="PK281_00565"
     CDS             25221..25583
                     /locus_tag="PK281_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY47856.1"
                     /translation="MIHIGEEIHAQLKSKRMKVVEFSRQINTNRNNVYDIFTRQSIDS
                     GLLCKISEALEFDFFRLYSLPLSQNKNIQQIPDPQEKLKEIEDIKFQNEKLLKENTIL
                     KERIKDKDLIIELLRSSL"
     gene            25621..26613
                     /gene="trpS"
                     /locus_tag="PK281_00570"
     CDS             25621..26613
                     /gene="trpS"
                     /locus_tag="PK281_00570"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOY47857.1"
                     /translation="METVVSGIRSTGNLHIGNYFGAVKNFLKMQHEQRCFFFIADMHA
                     LTTHPKPGDFKQSVKNVLVEYLAAGIDPEVSTIYIQSDVPQIAELYLLFNMYAYVGEL
                     ERCTSFKEKIRKHSDNVNAGLLTYPVLMAADIIIHKATKVPVGKDQEQHLEMARNFVN
                     RFNHLYQQDYFPEPYAYNFGEQLVKVPGLDGSGKMGKSEGEGNAIFLGEEPESIRKKV
                     MRAVTDSGPTEPFQAKAEPIANLFTLMSLVSEPETLAFFEESYNACSIRYGDMKKQLA
                     EDMIRFTAPIRERIKDIHANEDYLKKVMKSGGEKARESASATLKDVREIIGFMH"
     gene            26618..27115
                     /locus_tag="PK281_00575"
     CDS             26618..27115
                     /locus_tag="PK281_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070191 - methionine-R-sulfoxide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOY47858.1"
                     /translation="MAETLFFESSPTDRLKRYQELNTQLAALLSGEKDEIACLANTSA
                     AITEAFQFHWVGFYRVDGNNLVLGPFQGPVACSRIEHGKGVCGKAWKNNQTVCVENVH
                     EFPGHIACSLLSQSEIVVPIRNQSGVVCAVLDIDSSELADFSEVDQAGLEILCKMLGE
                     HLYSA"
     gene            27112..28782
                     /locus_tag="PK281_00580"
     CDS             27112..28782
                     /locus_tag="PK281_00580"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOY47859.1"
                     /translation="MRRKLSQVFEYSFMGLMLASCASRVPPNGGAKDTEAPVIVSSEP
                     DSAALNFQGKKVRFTFDEFIDLKDGGTGIVISPPLSIPPKYVVKGKTLEITFEEKLDS
                     ATTYSIILGKSITDISENNPFPTKTFAFSTGPVIDSLQMSGNVTDAYTNKAVKDAVVM
                     LYPTLDDSLPYKKLPRYFARTDASGNYQIEAIRAGEYLAFALSDANSNFLYDQPDEQI
                     GFLANPIRIDSNKTSGISFRMSPEKAKRQRLIKKEFELPGKITLKYALPVESLELKDY
                     SGNAQTIYPEFNIDRDSIVLWLKPAQTDSLNLICISKSLGQEKIDTLNYLPGQLRRKA
                     TVKKRNTSKVDTLLHFTNNLETGKIRPTENLELISNHPSKLKNRDQVYWVIGKDTTKA
                     QFIESEDRFTFKTQVKVPSELGNKAVLVMMPGAFEDIFSIQNDTTKTAVNRFVEDDLG
                     LLDFRIKSADSTSTFILELFTSKGKKLYSKQVKPGTPVVIEGLVPGSYSARIIEDLDQ
                     DGSWSKGRFSDRKQPERIRPYLGEITIRAGWDLELEWDLNAEKKLSVK"
     gene            complement(28784..32158)
                     /locus_tag="PK281_00585"
     CDS             complement(28784..32158)
                     /locus_tag="PK281_00585"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOY47860.1"
                     /translation="MKKLSFLFTCILFSSFVSLLHAQESNVTWISGDGINQPIHLRFQ
                     LGDYALQSVSTPNGPQSIVVAEGCTPILRKGAPDLPLFSASVIIPDLATLQAEVLSSH
                     QTELTNINIAPSKGNIKRTIDPNSISRIAGEVYSSNSLFPEAQVALRNPHILSDFRGQ
                     TVLFYPFQYNAQTHTLHVTDQIDVILRPVSGHTPINPLYRNADIRVNSVRQELYTNHF
                     VNFGTDRYTAITEQTNMLIITDPAFQTELEPLVQWKRESGMNVEVITLAEAGGNVAGI
                     TNAVHSRYTSNGLTYLLLVGDISQIPSPQSFGGKSDPTYGFILGNDTYPEVIVGRISA
                     ENSTHVATQVSKIVQYEKALLPTSNLDQVLVMASDQGPGDDNELDWEHERVMRDKLTS
                     YTYAEAFEQYDGSQGELDPDGNPSPSDVSTILNQGVGLINYTGHGSAVSFGTSGFSNS
                     EINQLQNNGLLPFIWSVGCVNGEFDNGTCFGEAWLRATRNGQLTGVVATFMSSINQSW
                     DPPMAAQDEMVDLLTGNVLTTTTRTFGGLSLNGCLKMNDEYGDAGDEMTATWHVFGDP
                     SLMVRTSIPLAMNVTHAATTALGSNGFMVSVDVENARVAITQNGILLGSSLVSGGQAW
                     VEFEPLTLMDPLLVTVTAFNRIPYQGPVQIENPSDAFILVSNHSFSEASGNGNQLADF
                     GETIYLNTTIQNVGGISANDVMGTLTSNDSYIIILQNQCPIGLVDAGNSIQSSDCFQF
                     QVSSSIQDQTLAHFIITYSDDAGQEWSQPITVMINAPELHITSVVITEVSGNGNGLPD
                     AGESIQIEFINNNAGHAATLDGNGTLTGSTNQFTVDQPSQSILSVDAANQLASNYTLT
                     ISSSVLAGSYIPATYTWMAGDYSITKDVIIHVGAKIEDAETNDFTHFAWQNADESPWT
                     IDQDIKYEGATSFKSGTISNNMTSSLIINWNVSVADTLRFMKKVSSEEGYDFLKFYVD
                     GNEIQSWSGVVDWSQEQFPITSGLHEFMWVYAKDEMISSNEDAAWIDYVVFPAGDDVP
                     EGFQASLNLSENSFQLWPNPSTDGQFILSGNQLISGKYSIRILDIGGRCIQTHSETLF
                     NSNSQWSMNLQAAAGLYFIEIRNPIGELSILKFTRN"
     gene            complement(32249..32929)
                     /locus_tag="PK281_00590"
     CDS             complement(32249..32929)
                     /locus_tag="PK281_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HOY47861.1"
                     /translation="MGGIYVIMILVMAASWFVSYRLKSKFKQYSEDFLQVGLSGKEIA
                     EKMLRDNGITDVKVTSVEGFLSDHYNPLEKTVNLSPEVYNGRSISSAAVAAHECGHAL
                     QHAKGYAWLSMRSALVPAVQFSSNIVQWILLAGVLLLNVFPFLLLAGVVLFALTTLFS
                     LITLPVEIDASRRALIWLESTRMTVGTEHDKAKDALQWAAMTYVVAAIGSIATLLYYV
                     SILNSRRN"
     gene            33173..33619
                     /locus_tag="PK281_00595"
     CDS             33173..33619
                     /locus_tag="PK281_00595"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOY47862.1"
                     /translation="MTLIKFNKPAQRPVTPMFSNLFQDLFDQELPGFRLGGNTNGLPA
                     VNISETEQNFHLELSSPGFTKEEIELSIDQDTLIVSGEKKSENEQTEKRYSRKEFSYQ
                     NFKRSFTLPENVDQEKISARFEHGILLIDLPKREQEAKTVKKISLN"
     gene            33706..34632
                     /locus_tag="PK281_00600"
     CDS             33706..34632
                     /locus_tag="PK281_00600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HOY47863.1"
                     /translation="MTEEAWFLIVNPQAGGGKGLKKKASIIAALQHAGLSHFVYETKC
                     PGDASTSVEEAVSKGYRRFVVAGGDGTLNEVANGILNQKYVPSDQLLLAQIPVGTGND
                     WRKSWNIPNSIEGSVALLKNPKSAKQDVASITFQANGKEEKLWFMNVAGCGFDAEVTL
                     SANAAKAKGKSGFFTYIGQLVKTLASYQEPEVQFRVDNQTHSAHLFSLLAGICKYAGN
                     AMKLVPQANPSDGLFHLTIASKISRLKVILNLPRLFSGSFTQLKEVKSLTCTEIEIQT
                     SGLPIQADGESIGHTPAIFVVHPLKLHIVIAP"
     gene            34633..34995
                     /locus_tag="PK281_00605"
     CDS             34633..34995
                     /locus_tag="PK281_00605"
                     /inference="COORDINATES: protein motif:HMM:NF025178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HOY47864.1"
                     /translation="MFSKKVTNWFQVFSSVEKAKEQVPLNKTIAVELGDKRICLARTE
                     KGFFALDEKCPHQNLPITHGGICENGTIVCPFHRYAWDLKTGREVERREGNIQLYPVE
                     ERAEGLFVGIETKKGWFS"
     gene            complement(34988..35938)
                     /locus_tag="PK281_00610"
     CDS             complement(34988..35938)
                     /locus_tag="PK281_00610"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HOY47865.1"
                     /translation="MISPTRIAVRKTGEKGFRLYTSTLKLLGDNQVNQILSEQEHLIP
                     FAFGTQWGLADTNGHVRIKPRFDEIIQIKDGKILVRQDSKYGVVSSNGKWIHKPDSDP
                     EKAKIHLLSTKEEKAKNRFPDFEKFEFQDGDSIIRVYKKGKVGFLDLRSNQLIAYPSF
                     EEASMFCYGKAVVKNENGYTLMDANGQLCPMQMFDYIQADRVPNLVFYSGIGAEQWMG
                     LLGTNCEILVPGSYKEIGHFYGDFAICSRSGEGYNYFDQKGDEVFPKQNFSKVENPAN
                     GFGILYDNSKWKIVNLNLADPRPILEFTDRSEFQLIYWAN"
     gene            complement(36064..36903)
                     /locus_tag="PK281_00615"
     CDS             complement(36064..36903)
                     /locus_tag="PK281_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018674659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOY47866.1"
                     /translation="MQLFPINTGHFKLDGGAMFGVVPKSLWSKSYPADANNLCSWAMR
                     CLLVQEGNRLVLIDNGMGNKQDEKFFSHYDLHGDDSLDASLKKAGFHRDDITDVFLTH
                     LHFDHCGGSIEWDDARSGYRPAFKNAVYWSNQEHWKWATEPNPRERASFLKENILPIQ
                     ESGQLKFLESNELFPGFSVEKVSGHTESMMLPVLSSNGHKIMYMADLLPSPVHVPVAW
                     VMAYDTRPLLTMQEKSSVLATCVSENRVLFFEHDKSIECATLQQTEKGIRVLDTFPLS
                     ALF"
     gene            37017..38381
                     /locus_tag="PK281_00620"
     CDS             37017..38381
                     /locus_tag="PK281_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013305212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HOY47867.1"
                     /translation="MKVHLIAIGGSAMHNLAIAMYKKGYEVSGSDDEVFEPSKSRLSA
                     LGLLPKEEGWFPEKIHAGLDAVILGMHARVDNPELIRAQELGLKIYSYPGYIYSQSTD
                     KTRVVIGGSHGKTSITAMILHALHTNQMDSDYLVGAQLKGFDTMVRITAEAPRILLEG
                     DEYLASPIERVPKFHLYYPHIALLSGVAWDHINVFPTFANYVEQFQIFTEKIEPNGTL
                     IFYDGDEELVKIAAQCRPDIRLQPYHEHASEIRDGVTYLVAGEERIPLKIFGKHNLQN
                     LNGAMLVCEELGMKRVDFYHAIQSFTGAAKRLELVYGGDGTSIFKDFAHSPSKLKATT
                     DAVREQFPNRKLIACMELHTFSSLNEEFLLEYKNSMAKADKAFIYFNPHTVAHKKLKP
                     ITPEQVRAAFGSENVTVYDDSARLLEDLKQENWSNSNLLMMSSGNFDGLNFEQLPSIL
                     GLKS"
     gene            complement(38378..38884)
                     /locus_tag="PK281_00625"
     CDS             complement(38378..38884)
                     /locus_tag="PK281_00625"
                     /inference="COORDINATES: protein motif:HMM:NF025227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HOY47868.1"
                     /translation="MKPETRGTLLILALSIIWKTLLLLTGWVDGPLGKYPFLPITAFM
                     LIGMYRGMEERRKLSYVESIPFKEMFKSGASIAALFAVLYSLFIYYYLFYLDTMFKVR
                     FAGERIEEFKSKGQNPDNFPFEMPWVLFTFIGTLVLGVFYSGAITRMIIKRNAMLEKR
                     SAKPSSKS"
     gene            complement(38887..>40379)
                     /gene="rho"
                     /locus_tag="PK281_00630"
     CDS             complement(38887..>40379)
                     /gene="rho"
                     /locus_tag="PK281_00630"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010177488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOY47869.1"
                     /translation="NERRDRTNRSLFDKRSDNLANYQPPAGQTPPAAAPQQQQTPPAQ
                     QYPQQNQQQNQNQQQNQGQNPQQQRGNWQNQNPNQNQQRQQMQRPQHQQRNPNQNSNP
                     NQNPNQQQPNVESNTPIDPLLNLDLTVPTEGVLEMMPDGYGFLRSSDYNYLASPDDVY
                     VSIAQVKLYGLKTGDTVDGGVRAPREGEKYFPLTRIDKINGRDPSYVRDRVPFDFLTP
                     MFPDEKLKLTGHKQGTMSTRVMDLFTPIGKGQRGLIVAQPKTGKTVLLKEVANAIADN
                     HPEVYLIILLIDERPEEVTDMARSVKAEVISSTFDEPAERHVKIANIVLEKAKRMVEC
                     GQDVVILLDSITRLARAYNTVQPASGKVLSGGVDANALHKPKRFFGAARKIEKGGSLT
                     ILATALIDTGSKMDEVIFEEFKGTGNMELQLDRKLANKRVFPAIDLTASSTRRDDLLV
                     DKETLQRMWILRNHLSDMNTVEAMEFIRQQMNGTISNEEFLIAMNG"
BASE COUNT        12728 a         7744 c         8046 g        11861 t
ORIGIN      
        1 tagctcttac gcacgtcagt ttttgggcag aattaataag cctgatgttg attttattca
       61 aggcatttct ccagcagttg caattgaaca aaaagtaaac tctaccaatc ctagatctac
      121 cgtcggaacc tcaacagaga tttacgacta catgaaactg ctttttgctc gaatcgggaa
      181 aaccatttct cctgtctccg gtgaacaagt gtttcgatct actccagctg atgtggtcga
      241 tttcatcaac acgcaaccca aaggagccaa agtctcgatc tacgcacctc tcaacaatga
      301 tcgcttcgac aataaaccgg ccaacctttc tttactccgg caattaggtt tttcgcgtat
      361 gcggattggt caagaactca agcgcattga agattgggaa aacgatggaa aaaccctgaa
      421 aggaaaaaca ttagaactcc tcatcgaccg ctttgtggtt aaccaagagg atgaagataa
      481 taatagccgc atctccgatt ctgttcaaac taccttctat gaaggtaata atgagtgcat
      541 tgtagaagtt gtcctggaag atggcaccat cctgaccaaa cgcatgaata atcggttcga
      601 agccgatgga attgtattcg aagaaccaag tgtacatctc tttagcttca acaacccttt
      661 tggtgcctgc aaaacgtgcg aaggttttgg aaccgttatt gggattgatg aaggactcgt
      721 tgttcccgat aaaagcttat ccgtttacga aggctgtgtg gctccatgga aaggtgaaaa
      781 aatgagcgaa tggcgcgaca attttgtcaa atatgccatg aaagttgatt ttccagtgca
      841 tcgcgcttat tatgacttag atgaaaatca actagatcaa ctttggaacg ggacgcccca
      901 tgccgaagga attaacgcat tcttccatta tgtagaagaa caagcataca aaattcaata
      961 tcgggtaatg ttatcgcgct accgcggaaa aacaagttgc cccgattgta aaggaacacg
     1021 attgagaaaa gatgccggat acgttcaagt gggtggcaaa agcatcatcg agttggtact
     1081 aatgtcaatc gatcgcgtaa aagcattctt cgatcaagtt gaattagatg aataccaggc
     1141 ccaagttgct aaacgtgttt tagttgaaat tcaaagccga cttggattct tatgtgatgt
     1201 tggattagga tacttaacgc taaaccgcct ctcgtctact ctttcgggtg gagaaagtca
     1261 acgtataaac ctagcaactt ctatcggctc tgctttagaa ggctcacttt acgtgttaga
     1321 tgagccaagt atcggacttc attctcgcga taccgatcgt ttaatcacca ttcttaaacg
     1381 attgagagat ttaggtaata ccgttatcgt ggttgaacac gatgaggaaa tcatgaaagc
     1441 ggctgatgtt cttatcgata tggggcctga agctggcaca cacggtggaa acgttgtgtt
     1501 ccaaggtgtt catgccgatc tcataactgc tggaagcagc ttaactgccg actacttaac
     1561 aggcagaaaa gaaattgcac ttcctgctaa aagtcgcaaa tggaatcagt ccattcaaat
     1621 taaaggagca cgccacaaca acctcaaaag tgtagatgca actttcccac ttggagtgat
     1681 gacggtcatt tcgggtgttt caggctcagg aaaaagttct ttggttaaca agattcttta
     1741 tccagcacta aaaaaaatgt tcgaaggtta ttccgagcaa accggacaat ttgatcgact
     1801 cgaaggtgat tacagaagca ttggagctgt agaatacatc gatcaaaacc caattgggaa
     1861 atcaagtcgg agtaatcctg ttacttatgt aaaggcttac gatgagattc gcacactcta
     1921 ttccatccaa cctgttgcaa aaacccgagg ttacaaacct tctgcttttt cctttaatgt
     1981 agagggcgga cgatgtgaag aatgcgaagg agaaggattt gttaccatct cgatgcagtt
     2041 catggccgac atcaaacttc aatgtgatgt atgcaatggg aaacgattca aagatgaaat
     2101 tctggaagta gaatacaacg gaaagaacat ctacaacatc cttgaaatga ctgttgaaga
     2161 tgccattaac ttcttcagca atggcaaaaa tgtagcaacc gatcaacgta ttgttgctaa
     2221 acttcaaccg cttgctgatg taggtttagg ttatgttcat ttaggacagg cgtcttccac
     2281 tctttcggga ggagaagctc aacgtatcaa gcttgcatcc tttctcacca aaggaagttc
     2341 tgcagttcat acccttttca tattcgacga accaacaact ggtttgcatt tccacgacat
     2401 ccgtaaactg ctggatgcct tcaatgcact cattgcacaa ggacattcac tcgttatcat
     2461 tgaacataat ctggatgtaa tcaaatgcgc agattatgtt attgatcttg gtccagaagg
     2521 tggcgatact ggtggacaag tagttttcga gggaacacct aaagatttag ctaagcgaga
     2581 agatttgtgg acaggtcgat atttgaaaga gaaattcagt tgattaatat tcgcttttat
     2641 ccctgctcga aagctaattc ccttgctacc tttgcatcat tatgaaacct tctcttccta
     2701 aaggaacccg cgatttctca ccacttgaaa ctgctcgtcg tcgttatatt ttcgaaacta
     2761 ttcgtgaatc gttcgagctt tatggctatc aatcgctcga aacacctgca atggagaacc
     2821 tttcaactct tacaggtaag tacggagaag aaggcgatcg cttgattttt aagattctca
     2881 attcaggtga ttttctgaaa gaagtaagag aacaacaaag tgatttagaa gctgtcaata
     2941 aagtgcttcc tctcataagt gaaaaagctc tccgctacga tttaacagtt ccttttgccc
     3001 gctatgttgc gatgaacaag gacaaactgg cttttccgtt tcgtcgctac caaatgcaac
     3061 ccgtttggag agccgatcgc ccgcaaaaag gacgttaccg agaattttgg caatgtgatg
     3121 ctgatgtggt tggaagtgct tcgcttatta atgaagcgga gttgattcaa atctacgacc
     3181 gggtattttc tgcattccga ttaccggtta aaattctcat taataaccgg aagattttaa
     3241 gtggtttggc tgaagttatt ggcgagcctg agagactgat cgatattact gtcgccatcg
     3301 ataaactcga taagatagga atagaaaaag tggagcagga aatgcgcgat aaaggggtga
     3361 gtgaactagc tatcgaaaag ctaaaaccac tgtttactat atcaggtgga atccaagaaa
     3421 aaacaagctt tctaagagat ttcctttctt catctgaaac aggtaaaaaa ggaattggag
     3481 aactcgaaac cttactcaat tatttccaag gtgaagatgg tttcaaatta gaaacatctt
     3541 cgctcgaatt ggatttaacc ttggctcgcg gattaaatta ttacaccgga actatcctgg
     3601 aagtaaaatc aaaagatgtg gaattcggaa gtatcggtgg cggcggtcgt tacgatgatt
     3661 taacaggcat tttcggactt cccggtgttc ccggagttgg aatttccttt ggtgcagacc
     3721 gaatttatga tgtgctactg atgcaggatt tatttcccgc ttcagccatt actggaacag
     3781 aagtattgat tgccaatttc accaaagaag atgagcgcgc tgctattccc gtgttacaat
     3841 tgttgcgagg agaaggcatc aaagcagaaa tctatcccga aatttctaaa ctgaagaagc
     3901 aattcgaata tgccgaaaag aaaggcattc ctttcttaat gatttatgga gaaggggaac
     3961 gagctgataa ttgcatcctc acgaaagaac tttcttctgg taaacaaatt tcagttccgc
     4021 tcgaaaaact gtcttttttc agagaactct tttgacacac gcagggattt tccaataatt
     4081 tcccaccttg cgattaccaa aatcaaccgc tactatgtca ggtcaagcta ttgatccatc
     4141 ccattgttat ttggaaagga ttcatcattt gctcaaatca ggagaacgaa tttttctttc
     4201 ggatgaagca aaagcaagta ttcaaaaagg tagaaattat ctcgatgaac gtttagcaaa
     4261 atcagatgca ccaatttatg gtatcaatac tggttttggc tccttgtgtg atgttcaaat
     4321 tagtcatgac caactggaac aacttcagga aaacctagta aaatcgcatg cttgcggaca
     4381 aggagaacct gttcctccag ctatagttaa ggcgatgtta ttgctcaaag ttcaatcgct
     4441 cacctatggt ttatcgggag ttcaagaaat taccgttcaa cgtttgatcg acttttacaa
     4501 cgagaatgtt ttaccggtag tttaccagca aggttcatta ggcgcatcgg gcgatttagc
     4561 acctttggcg catttgtgtt tacccttact aggcttaggt gaagtctggg tgaatggtat
     4621 tcgtcaacct gctgctagcg ttttagaacg ttttggctgg gaaccgatta aacttcaatc
     4681 caaagaaggt ttagcacttc tcaacggaac tcaatttatg aatgcgtatg gcgtgagtct
     4741 aagtgctgaa gccatgcgac ttttcaactg ggctaccgtt attgcttgta tttccataga
     4801 tgcttttgat ggtcgtccag atccgtttca tccgcgcatt catgaaattc gccaccatca
     4861 cggacaacaa gtcattgccg ctacagtaag acgtgtttta gaaggaagtg agattctaaa
     4921 tcaagacaaa gtacacgttc aagatccata ttcatttcgc tgcattcctc aggttttagg
     4981 tgcttcattc gatgtgttac gtcatgccga atcggttttc acgaacgaac taaatgctgt
     5041 aacagataac ccaactattt tccccgacga agatttgatt atctctgggg gaaatttcca
     5101 cggtcaacca ctagctatgg cgctcgatta tttatgcctt gcaatgcacg aagtgggaag
     5161 catttccgaa cgcagaactt atcaacttat tggtggaaaa cgcagcttgc ctccttttct
     5221 gattgctaag cccggcttga attcaggctt gatgattcca caatacacac aagcttctat
     5281 tgtgagtcag aacaaacatt tatgcatgcc tgcttctgcc gataccattg actcatccaa
     5341 cggacaagaa gatcacgtga gtatgggcgc aaatgctgca actaaggcat ggcgagtgat
     5401 ggaaaatctg gaaaaaattc tggcaattga gttactcaat gctgctcagg caatcgaatt
     5461 ccgtcgacct aaaaaatcat ctccaatcat cgaagatctg cttgttgcgt atcgtaaagt
     5521 agttccattt gtggatgaag atcgtttact ctacaccgac atcgaaaaca gtagacaatt
     5581 tcttaagaat catcatcttc cgctattcat atagcgaagc tgatagattc gatctttaac
     5641 ttctgagtct tcgggcattt gaattagcca attatccaaa acatagatag ctgaatcgga
     5701 tttgcccatg gtttcatagg caatggaaag atttatgtaa gctgttcgtt gtccgggatc
     5761 ttcttcgata atcgaattta atattttcac cgaagcggga taatcaccaa tgtcgtactt
     5821 ctcttttgcg agtaaaagct cttgttcggt ttgatgctgg agataccagt tccatccaat
     5881 gaaaagagaa actacagtca atacaaacag aatggataac aaagcaggct ttattaggct
     5941 ttcgcgttga tgattttctt cgtcagcttc tagaaattta tggaagtatg aatattgaaa
     6001 agcagcgcct gcaatggaaa ggatgatatg aaatacttct aatgcccaat cccacgattg
     6061 aggtaaaaaa atccacccca aaactaaaag cagaaatagc aaacctccaa ctagaaacgt
     6121 tagccaagtt agattgggct ttccaagttt tctaaaatta attccccata gaattactgc
     6181 cggtagaact aaacagaaat aacttataac tccaatatag agcggattcc agaccttttt
     6241 cacagctata aattactcag taaaaataaa aagctccggg aatgattcca actgtccaag
     6301 aacgcagatg ctcacccgaa atagctttat aaactaaggc ttcaccagct tgttggtaat
     6361 cgcgtgcatc acttgcccaa attgtcgaat cgattgcatt atatcctaaa ccgtaaaaca
     6421 aacgagaacc ttgtgctata aaaggacttg taggtaattc taaggcatta acatccaact
     6481 taaaaacacc tttgttcaga tagatcaaag aagaagcatc caaaatggag ttcaacttac
     6541 ttggatgatc cagagttgat aactgaaaac tcttcagcac cgaacgactt accggatcaa
     6601 tacaccataa gcttcctccc gtttcaacag caggaggaat atgtccttca cacaaaaccc
     6661 aaatacgatg attcttatcc attatcaggt ctttaggagc atcaccaaca gtaatagaat
     6721 caacaaataa attcgtttct ggattaagca gcataagctt acctcgcgtt acattactaa
     6781 tccaaattag gttttgagga tcttttatca tgccttcgct ccaaccagga aaaggaattt
     6841 cactcataag tgttccggag gtcgaattta ttacgtgaac tgtgtttgca tataaatcac
     6901 tcacataaac ccgattcgaa taaggaagca aataccgtgg tgattgtata cccgttacag
     6961 gacttgtggc tacaaacgtg ttcttgttaa cgcgttcaat cttttgggaa ttattcacta
     7021 ctaaccataa ctcatctccc accatagcaa gtgattgaaa cacgtctcct aaaggacgct
     7081 ggttagcctg ctgaaataca tcagcactga cagttcccga tttaagatcg attcgtgtga
     7141 gtgatgcatt tccccattga aaattccctt cgttcaaaat gagagctgtt ttacttactt
     7201 caactggaac aataggagtc ggctctggat catccttttt gcatgatacc acaaacatca
     7261 gcaccccaat caacaataac aactttctca tttctgaatg atgaaacgtt tacgaattac
     7321 ttgttgattg ttgctgagaa gcaaaatgta aattcctgct ttcaacgaat ggacatcaat
     7381 cgaaaagccg tcatcctgct tttcaattgc ttgattggag caagttcgtc cgtcggaaga
     7441 ataaacactt gccatccaat ttcccgaaat tgacttatcg catttcactt gcaaaaaagt
     7501 tgaagcgggc tgaggatata aaactatcgg aggctccgaa tgtgttgaga aatcaatggc
     7561 tgcaggatca ttattgtgta tgactccaac cgcatccaaa tcaaaacctg atgacataaa
     7621 tggagttggg aaaggatcgt ttaccattct tccttcggaa tcgcgcgtgc aaaattgagg
     7681 aagaatacta cccaccacat ctacaattcg aacatgagtt atggaattga tatccaaacc
     7741 cgaaatgcta tctaattctt gtaagtcgaa tggagttcca tataacatga catatttccc
     7801 agccagatta ttcaagttct ttgcatcaat atagctgaaa ccagcaatct gctgagaagt
     7861 atcggttagc gaggctgctg gaaaacgaaa gaaattttca ccatccgaac tcacttcaac
     7921 aaaacctaat tcgagaaatg aaccatcaaa actattttca aaaaccgcaa aatccggtcc
     7981 cgcgccattt cgaatactat gagcaaattg taaagtagca gaacctccat cacccaaact
     8041 tactactgga ttttcacctg ctttccctaa ggcataagct tctattccac tacttacata
     8101 acccaaagaa tcaacaccca tttgcatcca actacgctgc acaactgccg aatttgccca
     8161 ggcaacaaaa gatatactac tcacatgaat agccaacgaa cctggttgtc ctgctggtgg
     8221 atcaaattgc gctttcgtaa gtagcggaat tgcaaatagt aaaaagaaga gtatcgttct
     8281 cattagtgct gaactacaaa agtacgtgta tcagctacgc catcagcatg taaatgaatg
     8341 agataaagcc cgctagctaa ttctgaaaca ttaacatgtt ccgtaacaga attacttgaa
     8401 ccattcttca ccaacattcc agaagatgaa taaatactgt aatcaattcg ttccggattt
     8461 acttgacgaa tactcaatat ttcagcaacc ggattaggga aaacggaaag cgtcgatttt
     8521 gctattgtgt tgatggagtt aggtacaaca ttcactttaa aatcatctaa acagaaatat
     8581 ttgggtgtat tgattccaaa ttctccaaca tcgctcgatt caaatgaaaa cacaatcgaa
     8641 tcgactacac ccatggctgt taaatctaca cgtaaccact ctcgctgaat ataatccaag
     8701 ctattatctt caaatgtgaa atctgctaga tgaacaacta catctgttcc tgtagcttcg
     8761 ccattacgaa ttccagtaaa agtgactttg aaaaaatctg gatcattccc agaaacacca
     8821 ccaaacttct tagaaaaccc atcaccatcg cgcatgttat tgaaagcaaa tgtgttattg
     8881 gtaatataga gttcgatggg ttcgaatgca tagagtccgt ttgcatttcc aggcacgaat
     8941 ctgccatcat cgtatgcaat tgcataatta gctgaaccat cattgcctga tgcagcttta
     9001 cacgaataca tattcaagta ccctgatgta accgaatcgg tcatattact ataggcaaat
     9061 ccatccaacc aatatccacc aaatgctgta tcccagcgag ttggaaagaa agcattatga
     9121 cttgtaaatc cggttgatac accatcggtt ccaacccacc aagaatcggg acttagtgtt
     9181 aaatcttcaa aatcgacggt agtttgtgta aaacctatca tgtttgaaat aaagatgaga
     9241 gtcgtaagaa atataatttt cattttgttg gattgggttg aaaagtgaaa gagatgccgg
     9301 tttgaaacga taacagaggc attgggcgcc aagcaataac ttgatatgat ttttgtagta
     9361 gattaaatgc atttaaaaag aaagaaaaat ggtgctcttt cagttcgagc tgcttcgata
     9421 caaatgcatt taataagaaa aaaccgggta gtgatttttc attatcactg gtgataaagc
     9481 gttctccggt ataggttgaa cttaattcga attgaaagcc tttccaccga taagacattc
     9541 gtgcattaca agtaaaatgc ggaacataaa ctaattgctt acccgattct actatacttc
     9601 ctatatctga tttttcggct gttacccaaa catattgggc attactgaaa aaatgaagcg
     9661 attgtttttt cttaaagtgg cgatggattt ctatcccagc atctagtcct ctgctatgaa
     9721 cttgtggcac attttccggt gtccaaaatg agttagacgg taaccaaaca atccaattct
     9781 tcacctgatt actaaacacg gaagccttac caagtaatac aaatgggagt ttctctacat
     9841 aatagtttag tccaaattca gatgctattc cactttctgg tcttaaagat aaattccctc
     9901 caggttgcca atacaaatca ttcaacgttg gcactcgaaa actacgagca acttggccgc
     9961 gaatagtaag cgttttaaat agtggatatg atgcagatat agatggaata aatggcacaa
    10021 attgtcgatt gaaccactcc tgccggcctt gtattaaggt gactaatttc cctttaaata
    10081 gctccgatcg aaaagaagta aaaccggctc ttcgttcttg atgaacagct gagcttccat
    10141 aaccttgtgc atgagcactt gaatagctga aatttcctcc tagctgaaat cgggaatgga
    10201 ttgatgggaa attctttcca aattcagtct cggctaaata agttttagca tgattggcgg
    10261 catgaagact taatagcgaa tcgttgaata agattccctc ctgaagatat cccgttttta
    10321 gttggatgtc gaatttactg ctcactcttt tccacgacaa cattccacgc caagtttcat
    10381 cttgttgtga agcagctgca ataggaatcg tttctattgg aggaatttgt cggattgaag
    10441 tctgccccca aactgaaagt tgcaattgac tgtatttacc tgttacccaa tacaaatctt
    10501 gcatccagcc tttcatcctt tgtgcagcgt ttaactgctt ctgtttagga tgaccaaata
    10561 ctgcattatt aataaagtga aaatcgttct caacctgtga ttggaacaat cttgtacgaa
    10621 aagaaatacg accaagcgta taggcaacat caacagctgt ttgcttaaat ccaaaacttc
    10681 caattgattg ctgaataagt aaatgattgt ttgaatgctc agagccgaga tgaatcgtcc
    10741 cgcctattgc acctgttccc caggaagcag caccagcacc cgattgcaaa ctaaaatggt
    10801 caaacaaaat agccggcacc agacttaaat ccatttgccc attcatacta ctttgtaagt
    10861 tcaaaccatt ccaaactaag gctgtatgag ctgcaccagt tccacgaatg gaaggagaag
    10921 agagtgttcc aggactatat tgacggatga aggaagccga atttcgactg agaagttctg
    10981 ctaaggattg tatgttcttt cccgcttgtt gagtggaaaa aacactttgc tgtaaaccag
    11041 gatttgaact tgggatacgg ttgtcttgaa cttgaaaagc aggaagctga tgaacagaat
    11101 ctttcgcagc agtttgtcca atcgacctaa ggccaatgag caaaaaaaag atgcatgtga
    11161 aaattctatt tttcatcgtt cttgtttatc cgcaggggaa aaacaaaaca ggaatggacg
    11221 aacagaattc ctccattggc tgaaaaagcc ctcctgttca ttgcctttct tcccgaaagc
    11281 acggaacata ttggattctg gcaggtcttc tgacttacct cacattcagc gccttcccat
    11341 aataacagtg gcattgagct gaatgctttt gatgaggatt acagcagcgg gtactgttcc
    11401 tgagtttcac aggattccct tttaatcata ccaaaggcat gaaccaaaaa gcggtcaaag
    11461 ttaagtcgta tatctgattt ttagaattaa aaaattgatg gataaaaatt catccttaaa
    11521 tcctttaccg ttaatgtaat tgagtccgga aatctttgta taaacttgca acttgaaaaa
    11581 cacacttatg ttaaaacgca tttcgttcct ttcaggagcc ctatcactca tgcttcttct
    11641 tcctgctact gcccaaaaac tggtagaaaa ggtggagaaa aaaggagatg agattgtcat
    11701 cccttatgaa aaatacaaac tcgataatgg gatgaccatc attatccacg tggataaaag
    11761 tgatccgatt gtccacactg atgtaacata tcatgttggt tcggcacgtg aacaaattgg
    11821 tcgttccgga tttgcgcatt tctttgaaca catgatgttt caaggttccg ataacgtggc
    11881 tgatgaagag cattttgcta tcgtaacaga agctggtgga acaatgaatg gtacgactaa
    11941 tctagatcgc actaattatt ttgaaacctt accaagcaac caacttgaaa cctcactttg
    12001 gttagaagct gatcgtatgg gattcttgtt agatgctgta acgcagcaaa aatttgaaat
    12061 ccaacgtgcg actgtaaaaa acgaacgtgg acagaattac gataaccgtc cttatggatt
    12121 ggttggagaa cgcacaaacg aagcgttata tccttatggt catccatact cttggccaac
    12181 aattggttat ttgaaagatc ttgaccgagt tacggttgat gatttaaaac gattcttttt
    12241 acgttggtat ggtccgaaca acgcggtttt aactgttgca gggaatgtag atccagcaga
    12301 agttgttcgt ttggctcaaa aatactttgg tagcattcca cgcggaccag aagtaaaacc
    12361 aatggataaa atgccggtaa cgttagataa agaccgttac atttcaatgg aagacaacat
    12421 tcgttttccg atgattcaaa tgacttggcc agcagtttct tctggaagtg atgatgagct
    12481 tcctttggat ttattggcag aattgattgg aggagataaa acatcaattc tttatcagaa
    12541 tctaatcaaa acaaatttag ctgttcaggc tcgtgcatat aatccagcag ctgaattagc
    12601 cggaacgttt gtcgtgaacg tattgccttt cccaggtaaa acactagccg atatggagaa
    12661 aattattcgc gatgcatttg ttgaatttga aaaacgtgga gtaaaagatg aggatctaat
    12721 tcgaatcaaa aattctcgtg aagctcaaat gattcaaagt ttagaaagtg ttagtggtaa
    12781 agcttccaaa ttagcttata acgaaacatt taaaggaaat ccgaattaca tcaaaaagga
    12841 aattgatcaa cttcaaaaac ttacaaaaga agatgtaatc cgcgtttacg agaaatacat
    12901 caaaggcaaa ccagcagtta ttactagcgt tgtacctaaa ggtcaaaaag aactaattgc
    12961 tcacgaagat aacttcacac ctaaacgtgc aacagaagca gaaagcgcag aaggtagcga
    13021 atacaaaaac ttgagttatg taaaagcaaa agatgttttc gatcgtagca aacacccagc
    13081 accgggagct aatccagtaa ttcgccttcc agaattctgg aaagaaacaa gtgctaatgg
    13141 actacagatt atcggaacac aggacaacga tttacctatc gtttctatgt taatttcatt
    13201 ccctgcaggt cacgcagctg aaaatccagc aaaagcaggt ttgtcgaacc ttttgacaga
    13261 tgtgatgagt gaatcaacgc tcaaatattc ttctgaagaa attacttctg aacttgaaaa
    13321 attaggttcc gaaatcagca tttcatccga tcgcgaatat gtgtatgtaa acgtcttttc
    13381 tctgaagaaa aaccttgctc gtacgattga attattggaa gaaaaacttt ttcatccaaa
    13441 atttgatgaa gaggaattca acactgcaaa agcacaggaa attgctgcaa ttgataacaa
    13501 tgcaacacgt gcagctacca tcgcggataa tgtttactct aagctattat atggtgacca
    13561 tatttttgga attccagtta ttggtacaaa agctagcttg gaaggaatca cattgcagga
    13621 tgtgaaagat ttttatgcag ctcaaatcat tccagctaaa gctaaattgg tagcagttgg
    13681 acaaatcact agcaaagaat tgaatactgc tgttccattt ttgttgaatt ggaaagcatc
    13741 agcatcgcct gttgaaatta aacaaccagc aacgcctgca attgcagcta ctactattta
    13801 tttcgtaaat aaagacaaag caccacaatc agaaatccgt atcggttata tggctttacc
    13861 ttatgatgca gatggcgaat actatcgtgc tggtgtgatg aattacacgt tgggtggcaa
    13921 ctttaatagt cgaattaact tgatgttacg tgagaaaaag ggatatacct atggtgctcg
    13981 tacaggattt agcggaacac tttaccgtgg ttcattcacc gcatctgccg gagtaaaagc
    14041 tgaaaaaaca gatagttcat tgatggattt cttctccgaa atgaatgcat tccgtgcttc
    14101 tggtattact cctgaagagt tgaatttcac taagaattct cttggtcaac gtgatgcttt
    14161 gaaatacgaa actcctttcc aaaaggcatt attccttcaa aatttgttag tgtataacct
    14221 agacaaaaac tacatcgata aacaaagtgc cattttaggt agtttgacta aaaatgacat
    14281 cgatggattg gcgaaacgct atttacctac tgataaaatg attgttgtag ttgttggtga
    14341 taaagccagt aactgggata aaattaaagc cttaggatat ccagttgtag aattggatac
    14401 agaaggaaat atcgtcaagt aagaagtatt aaatcataaa aaaggcagcc tgaaagctgc
    14461 ctttttttat ttccagatat cttgttcttc cagatttact ttttccggaa agaagtatgt
    14521 ggcactactt tcaaatgatt cagtaaaatc cgaaactaag aaacgtagat gcggattgtt
    14581 ttgagtagaa agtaaatctg attcggctag taaattcttt acagttaaag ccaccatttc
    14641 tgccgagtca actacatcaa cagagccttt atagaactct tcaatttctt tacgaataag
    14701 aggataatgg gtgcaaccta gaataattgc ttcaatattt tgtagtgatt tcttttctaa
    14761 gtaggaatga attactgttc gagaaatacg gttattgaaa aacccttctt cgatcattgg
    14821 agcaagaagt ggagttgcaa gagaagtaac ttctgtatga gcactgtact tctttatttt
    14881 cctaggataa atacccgatt gaatagttcc gcgagtagca ataacgccta ctcgtccatt
    14941 aggatagcgt tctgcaacaa cttgagatgc aggatcgatc acattaacta caatggcttt
    15001 attccctacg aattttgtga ctgctttgaa agcatgagcg gatgctgtat tacaggcaat
    15061 tacaatcatt ttacagcctt gcgagagcaa atattctgcg atacctaaac cataagattc
    15121 gatacgtgca ggtgatttat caccataagg gaaatgtgct gtatcaccaa aatagataag
    15181 tgattcgtga ggaagcagtt gatgaacagc cgcagctaca gttaatcctc caataccaga
    15241 atcgaatatg ccaattgctg cgttcttatc catatacaag aataaaaaaa gcaccccgat
    15301 tggagtgctt taaagaagtg ttttttttat tctcaaggaa gattgagttt tttcttcacc
    15361 ataacaagaa tgttatcgct ttcttcccag aacaataaag aacctgctga agtatcaaaa
    15421 atgtaggtat atccattttc cttagcaact gtctcaattg ctttcttggc ttttgaaaca
    15481 attggttcaa tgagagcttg ttcttgttgc tctaaagatt gctgagcttg ttgctgaaaa
    15541 ttttgaatac gagtacctaa actagtaagc tcttcttctt tatccttctt tactaattca
    15601 gataatttag gagcttcgtt ggtgtatttt tcttgtttag tacgatactc ggtcatcatt
    15661 tcttgaagtt gtttttcaac gtctttcgtg atggaatcca ttttcgctgc agcagtttta
    15721 cgctcaggca taagacttaa taattcggcc gaattaatat gtccaaattt atttgatttc
    15781 tgcgccaatg caggcgttcc gatcaaggca gtggcaaata atgccagggc gatcaattgc
    15841 ttcattcgtt tttaattagt tattttttat tcgttggtgg agttggggaa gaaggactag
    15901 atcctcctcc tttatcgtta ctatcctgat catctccttt ttgacctgga gtaaacccta
    15961 acgattccaa gacttggtcg ctgatatcat atttaggatt tgtgaaaagc attgtcaaat
    16021 cacttgattt atcaaatact acagcgtaat ttttagttgt agcaatttct ttaatcgcgt
    16081 tatagatttt atcttgaaca ggcttaatca aatcttgacg ttttttaaga agatctcctc
    16141 ctttaccaaa acgttgcttt tgtaattctt tagcagcttg ttctttatcg ataatttctt
    16201 cttcacgttt cttcttcata tcgtcagtca gaagaataga ttctgcctga aaatctttgt
    16261 acattttatc aatctcagca aattttactt caatttcttt ttgccattgg attgatatac
    16321 gatctaattg ctgttgcgca gatttatatt ctggaaggtt ttccaaaata tattgagtgt
    16381 cgacgtaagc aaatttttgt gccgaaacgc ttgaagatac agcgaaaaat accagaagcg
    16441 ctaagatgag ctttttcata accggctgga ttgagggtgc aaaagtaatc atttctataa
    16501 ggtttgttgg ccgcaaaaca acaatcgtgc taagcagcaa tttcgaagtc cttcatttgg
    16561 attaattctg acgcaataga acattctcta attgaagttt tgtcctatta tgaagtgaaa
    16621 ttggctttta ggcattcccg aaaaacctgg aacttcatcg aatcggaatc cataatcaag
    16681 tccaagtaat ccaaacatgg gcaaaaatac acgcacacca acacctgccg aacgtttgat
    16741 actaaaagga ttgaaatctt tgaagtttgt ccaagcattc ccaccttcta gaaatcctaa
    16801 agcaaaaaca gtagcggagg gatttaatga aatgggatat ctcaattcca tcgtatatct
    16861 actataaatt ggtgcatcac ctactcctcc agttgctcct tttgaaatag agccgtcctg
    16921 atagccacgt aatccaacga tttcagcacc taagaattgt gctccccaag tcaatccact
    16981 taaacctgtt cctccgtatc ggaagcgctc gaatggtgac aagcctttgt tctgattata
    17041 taagccaaca aaaccaaagt ttgcacgagt cattaatact aatgtattct tatttcgaga
    17101 aagcggcgta aaccactgag catcaaactt ccatttgtgg aactcaaccc atttatatcg
    17161 ttgttcgagc gttactaact cactagcagg agtattatta ataaccgaat atggtggagt
    17221 gaattgtata gataggctga tgttagagcc cgattttgga taaataggtc catcaatcga
    17281 gtttctagat aaagtggttg tgaaacttaa gttattagca aacccattag ctagttcaaa
    17341 tgcagttgaa ttgactaatt catagtattg gaaactaacc gattgctgca aagtgaaaaa
    17401 gtcatcaggc caacgtaatc ttctaccata cccaaggatg atacctgtat ttttagtaag
    17461 actacgagtt ggatcattct tagctgcacc atttccataa attgaatgat aggcagtaat
    17521 tgttaaagaa ttaggttttt ttccacctaa ccaaggctct gtaaaagaca aattataact
    17581 ctggaatgca cgacctgtag aagaagcatt taaagataag cgttgaccat caccagaagg
    17641 taaaggacgc caagcatctt tcttgaagaa tcttcttgca ctaaaattat tgaaagcaag
    17701 accaaggttt ccaattaatc ttcctgcacc ccatcctcca gaaagctgta attggtcgga
    17761 aggtttttcc tcgactttgt attcaatatc aacggttcca tcttgcggat ttggaacagg
    17821 attaacgccc attttttcag gatcgaaata tcctagttga gataactcac gttgggtacg
    17881 aataatatca gaacgtcgaa ataattgccc aggtcgagtt ctaatttctc gcattactac
    17941 atggtcattc gttttatcat ttccagttac tgttacacga tttattcggg cttgttttcc
    18001 ttcataaata cgcatctcaa tatcgaccga atcattgtcg acaaaggttt ctacgggagt
    18061 tacttggaaa aataaatgcc catcattcat gtataaagaa gagacatccg attcagatgc
    18121 attcatgaaa agattttgct ccaaagtagc ttggttgtat atctcccctt ttttaattcc
    18181 taaaatatca ctgagttgtt tggatgtgta tttgacatta ccaacccaat tgatatttct
    18241 gaaaaaatat tgacgccctt catcaatttg aatatcaata ttgatgtgtt ttgcatcgta
    18301 acgataaacg gtgtctttta caatgcgagc atctttgaaa cctttatcaa agtaaacagc
    18361 tagaattttc gatttgtctt ctacatagtt gtcttccatg tatttagacg ttgtaaacaa
    18421 actataccaa ggattttctt tggtattttt cattttcttt ttcagctttc tatcagtcac
    18481 gaattgattg ccattaatgt ttatttgatg aatcttaact cgtttgttct tattcaccaa
    18541 aatattcatc ataactccac cacctggtat tgtatcgttg gttgtattta catcaacagc
    18601 aacattcata taccctttat caataaagaa ttcccgaaca gtatttcgag tattcaccat
    18661 caaattttca ttaactggct ttcctcgaat gagcttaatc tgctcacgta aatcgtcagc
    18721 ttcattcttt tttacaccca caaaacggaa tctagatagt cgaggtcttt cttctagtag
    18781 aataatcaaa gagattttat ccccttctat tttagcagtt ttaacttcaa ttccagtaaa
    18841 aagcccttgt ttccatagtg cttcaattgc actactaatc cgatcacccg gaatcataat
    18901 cttatcacct ttattcaagc cggataataa gcgcagtata ttctggtcaa gattactgtt
    18961 gtcctttgtt ttaaaggata tatcagcaat ctcatattct ttaggggcgc tataacttat
    19021 gccaataggg ttattgtcgg gaataacctg agcaaacaca atttgctgag aagtgagaaa
    19081 acagaaaagg agaaataatc ggaaataaag acgcgtcatg atggggtatt caattgttcg
    19141 cttgttttgc caaagcggcg ttccctttgt tgatagctga caatggctgc atagaggtct
    19201 tcttttctga aatctggcca tagcttatcc gtaaaataca attcagcata agagatttgc
    19261 cagagtaaaa agttactaat gcggtgttct cctgatgtac gaatcagcaa ttctggatca
    19321 ggcattccag aagtatttag gtaattagca ataagtttct ctgaaatatc ttccgttttt
    19381 atttttccaa tttgaacatc ggaagtaatt tgttttacag cttgggtaat ttcccatctt
    19441 gaactatagc taagtgctaa tacaagtgtc attcgagaat taccagctgt tttcttttta
    19501 gcttcttcga gttcccgacg acattttccc ggtaaggagt caatatcacc aatagattcg
    19561 agtcgaatat tgttcttcat caatgtggct gtttccttac tgattgtgct aaccaataaa
    19621 gtcatcaaag cattgatttc atatttagga cgattccaat tctcggttga aaatgcatac
    19681 atagttaagt atgaaatacc taactcggca catgcttcca cagtttcgcg aactgctttc
    19741 acaccatttt cgtgaccaaa tgttctgagt ttaccttgca tttttgccca acggccattt
    19801 ccgtccatga taacggcaat atggcttggt aacgtatgca gattgatatc ttctttattc
    19861 actttctttg cttgggccat atgattgcat acagcattaa cgcgcaaaga taatggatta
    19921 cttatatgcc gcgcattcgg cttttcgagg gactatttta taagtaattg tcagtccagc
    19981 aaacgagtac caatctttat tcgttgaatt tcctcgttgt cgtccagcat taattttacc
    20041 gggattgaca agagatctat ctgcaagcaa ggcagctaat tcaccatgag tagcaaaaag
    20101 tacatctgga tcagcgtaag ttgtacttac atcgtctaag taatcggtga acgttttacg
    20161 taatccccac tctaagctaa atcctaaacg accaaacgca tgaaatttaa cgccaaatcc
    20221 aaatggaata ttggcaccca tgagtgagta ttgtttacga tcggcataaa caggagaacc
    20281 ttgtccttcg gtaccaagtg gttgaagagc aatccattct ccttgaaaat cagctttagg
    20341 attgaagcgg aaaagaccta acccaccaaa aatatatgga gttgctggag tttcgggatt
    20401 tccaatttcg taattaaaaa agttaaagtg gagtaatcca gcaaattcaa tgatttgaga
    20461 acgaaacgaa agatttctca aaatttgatt gtcttcccca gaacgagcat cttcagcctg
    20521 aacactgcca tataaaccat gaatttccca agcaaagcga cgactagtat tatatcggta
    20581 tactaatcca ccaccaggac gaaccataaa aaattgcttt ccgggattta attcacccat
    20641 gtaataggaa actccaccaa aaagcccaag ttcagaatat ctattaaata cctgagaatt
    20701 agcagaaaac gaagttgcta atccaattaa acttagaaaa atccatttac gcatacatag
    20761 aggtatacag agaataacgt aaatgagtta ccggaatttt ggaaggattg attttcctga
    20821 tttcagtcgg taagtcatgg ttacaattcc aaacatgtag gcatcgcgat ctgttggatc
    20881 tccacgttgt tgaccagttg cagccgatcc tttaataaaa tcgggtgaga acgggtttgc
    20941 tctgttagat aatgcagcag caacaggacc gtttttatcg gcaattaatg ttggagagaa
    21001 ataagttgta cttacgtcat caagataatc ggtagttgtg atacgcattc catattcaaa
    21061 accgattgtc catcttttat cttgcattgg aacacggaaa ccgaatccaa ttgggaaaca
    21121 catttgaaag cgtttgtaag gagcacgaga tgcagcaatc ccttgtcctt cggtgcctag
    21181 tggctgaagt gcccaccaac gacctccgag gagtgcttta ggattaaagt aaaaacctcc
    21241 aacgccagca aacaagtata cgttcaagtc aattccactt tttcctctta ccccttgaag
    21301 tctatagata cttttagatg acttcaactg tacaattgat agctcaactt gcaaggatga
    21361 ttcaaaaatt ggagaacgaa aactcaagtt tctgtttcta cggaactctt cacgtgttaa
    21421 gctatctcta cctgcaacac gtccataatt caaattaaat ttgaaagaga gaacaggatt
    21481 tttacgataa cgaaaaccta agtgaactgc aggacgagta aggatcattt ccaaatcctt
    21541 tatcccatta gtaccaaatt ggtcggcacc accaagttct cctaagaagt tagtaaaccc
    21601 gataccgtat gtgctttcgc aacgatcgag aaaacgtcct tttccttttc gctgagcttg
    21661 agcagaaatc gaaatgaaaa gcagtaaaac ggtaaggatg gatagcttac gcattggatt
    21721 agtgttattg gcaattaggg caaatatacc aaaattgaaa tgctattagt acgaagaaat
    21781 gaaaaccttt ttgagtatgg taattattaa tttcttcgat cgattcccca catgagtttt
    21841 tctctcaatg tttttaaaaa tggttgttct ggtttgcgtg ctaatcgaat tgaaaaaggt
    21901 gctttattga tagttatttc tgttttcgct ggaattgatg cagaacgtga atcgagcgct
    21961 aataggaaat tttttccacg aacttccaca tcaattgata agctgtgttc atctgtaata
    22021 actaatggcc taacattaag attatgagga gcaataggag tgattacaaa actgggtgat
    22081 tcgggcatta atataggacc accacaactt aatgaatatc ctgtagatcc agtaggagaa
    22141 gctacgatca atccatctgc ccagtatgaa ttcaagtgtt ctccatttaa tcgggcatga
    22201 acagtaatca tagtagaact ctctttctta tgaagagtaa tatcattcaa ggcataaggg
    22261 aaatccgcaa aggggccacc attcattttt agttgaagaa cagagcgttt ttcaagcaaa
    22321 taattctggt ttaaagctgc ttcaacagcc ttggcaattt catcttgaga atagccggta
    22381 agaaatccta atctaccagt attgataccg acaagaggta tttcggtgtt tctaattaat
    22441 gttactgatt cgagtaatgt tccatcgcca ccgatagtaa tcatgcagga tgttcgatta
    22501 tctaaatcgt cttgattctt aaaccgtgaa aaattagcac ctattggcat taaactttga
    22561 gatgctttga aaaaaggctc gagtatttga acttgaacac ctgattgatg caacagaaaa
    22621 tataattcct taataacagg agctctgctt tcggtaaatg aacggccaaa aacggctatt
    22681 tgcattttgc ttaattgaat aaagcgaatg taggtatcct aattcgaaaa gaatgacaaa
    22741 acgaattaga aaattgaaat tacatgttga tgtaattcat aaacaagtcg aacctgtttc
    22801 taagatcttc cgaaaactct gtttgctgga aagtcgcttt aacggtaatg ttgtagcgag
    22861 caaaggaagc aagaatacca cgaatgtctt cgcgattcac ttttatggtt aactcaattt
    22921 gagtagaatc tggattattg ctaacagaaa gacttagaat cttcgtgtcg ttttcctcta
    22981 caattcgagc aatctgagat aaagaatagt cgttttgatt gagttcaaga acgataatcc
    23041 cacctggatt ttgtacagaa acagtagctg caaatttttc taccaaagaa cgcagactaa
    23101 tatttccgag gtatttgttt tcggcatcca aaacaggaat cagagttaaa tgcatgcgtg
    23161 cagctaactt caacacttca tagatgtgct ccgattcgta aacaaaaggg cgaataagcg
    23221 aaagtggatg gtttccaaga gcttcctcag gagcattaag gtcaaggata tcagtatccg
    23281 agatcaatcc taagaattcc gaattattga caataggcaa atgtgctaca cggaactcat
    23341 ccatccaatg aagcgcttta atgccactgt cggatgtttt aagtggagga atagtatctg
    23401 atattagatc ctgtgcaagc atgaatggaa actaaatctt gttgttttga aaacgtttgt
    23461 agttgaaaat tgtttcacct tgacggcaaa cgtagcgaat agaaatatct tctccaacgt
    23521 gcaacaggtg tttcgtagat ttgcccctaa ataataacac atgactctac taagtgttaa
    23581 catcaataag attgcgcttt taaggaattc gagaggttca aattatccca atgttgctga
    23641 tacagcttta aatttggaac gtttcggagc tcaaggaatt acgatacatc ctaggccaga
    23701 tcaacggcat gcaacatacg aggatgtttt tacacttaag ccgattctaa aaacagaatt
    23761 taatgtggaa ggttatcctt ctcgtacttt tctggatgtg gtattaaagg caaagccaca
    23821 tcaagttaca ctagtccctg accctccaga tgctttgact tcaaacgccg gttgggacac
    23881 gattaaagaa ttttcttttt tgcaagaagt tgtgacagaa ttacatcagc aaggcattcg
    23941 agtttcccta ttcatggata cgaatctcga tttatacgat caagctgcta aaacaggaac
    24001 agaccgaata gaattgtata ccgaagcata tgcgagacat tattcttcag caccagagca
    24061 agcgatcgaa ccctatataa aagctgcatt aagagcaaaa caattaggat taggattgaa
    24121 tgctggacat gatttgaata gccaaaatct aaaatacttt gcccaacaca ttccagattt
    24181 attggaggta tcaatcggtc acgccttaat ttctgaagca ctttatttag gattagaaaa
    24241 tacagttcaa atttatcttc gccagctaca tgaagctatt tgagcgacat ttcggaacag
    24301 gagaaacggt agttattctt catggattgt ttggacaaag tgataattgg accagcatag
    24361 caagaaaact aggagagaaa tattgtgtat acacgtttga tttaaggaat catggacagt
    24421 cggggcattc agatgaaatg aattaccgtg tcatggcatc tgatgtatta gaaacgattg
    24481 atggtttagg cttggataaa attcatttga ttggacatag catgggagga aaagtggcga
    24541 tgctgtttgg cttaacctac ccagaaaggt tgaattcatt ggtcatagct gatattggac
    24601 ctcgatatta taaaccacat cacagtgata taataactgg actatcaaat attgatttgt
    24661 cgagtttaaa atcgagacaa gaagcagaga atcaagtact ggatttcatt cccgacttcg
    24721 gaacacgaca atttttattg aagaatttat atcgcttggg agaaaatcaa tttgcttggc
    24781 gatttaactt accagttatc agtagaacta ttgaaaatat tggcgaggca atagattcgg
    24841 gactttgtca aatagctact ttattttatc gtggttcaac aagctcttat ataaaacccg
    24901 aagatattcc tgctatttta aatcaattcc cacatgctca atttgaagta atgcaggatg
    24961 cagggcattg gctgcatgca gaaaaaccag tagaatttat tgaaacgatt gagaactgga
    25021 tttcgtccca atccacatga attaagctct agtttccaac ctcatattta agtcgataaa
    25081 gttacatatt tgtaaataaa agtacatttt gccaaataat taatggcaat taagtgaaca
    25141 atttttcgaa ttttcatttt cccttacata atattttgca aatttgaatt caaactttat
    25201 ttaactgatt atttactgat atgattcaca ttggggaaga aatacacgcg cagctgaaaa
    25261 gcaagcgaat gaaagttgtc gagttttcga gacaaattaa tacgaataga aataatgttt
    25321 atgatatttt cacccggcag agtatagatt ctggcttatt atgtaaaata tcggaagcat
    25381 tagaatttga tttctttcgc ttgtatagtt tgcctttatc tcaaaataaa aacattcaac
    25441 aaataccaga tccacaagaa aaattgaaag aaatagagga tattaaattt caaaacgaga
    25501 agctattaaa agagaataca atcttaaaag agcgaataaa ggataaggat ttaataatag
    25561 agcttctcag atcatcgtta taatcgtttg cgctgcaaca atttcgatta gttttgcagc
    25621 atggaaacgg ttgttagcgg tattaggagt accggaaatc ttcacattgg aaattacttt
    25681 ggagcggtaa agaacttctt aaagatgcag cacgagcagc gctgtttttt ctttatagca
    25741 gatatgcatg cgctcactac acatcctaaa ccgggtgatt tcaagcagag tgtaaagaat
    25801 gttttggttg agtatttagc tgctggaatt gatccggaag taagtactat ttatatccaa
    25861 agtgatgtac ctcagattgc agaattgtat ttgttattta acatgtatgc ctatgtgggt
    25921 gaattagagc ggtgcacatc gtttaaggaa aaaatcagaa agcactccga caatgttaat
    25981 gcgggtttac tcacatatcc tgtattgatg gcggcagata tcatcattca taaagccacg
    26041 aaagttcctg ttggaaaaga tcaggagcaa cacttagaaa tggcgagaaa tttcgtcaat
    26101 cgctttaacc atttatatca gcaagattat ttccctgaac cgtatgccta taattttgga
    26161 gagcaattgg taaaagttcc aggattggat ggaagtggga agatgggaaa aagtgaaggt
    26221 gaaggaaatg caatattttt aggagaggag cctgaaagta ttcgtaaaaa agtaatgcga
    26281 gcagttaccg atagtggacc aaccgaacca ttccaagcga aagcggaacc gatagccaat
    26341 ttatttacgt tgatgtcgtt agtttctgaa cccgaaactt tggcgttttt tgaagaaagc
    26401 tataatgctt gttccattcg gtatggtgat atgaaaaaac aattggccga agatatgatt
    26461 cgtttcacag cacctattcg tgaacgaatt aaagacatcc atgccaatga ggattacttg
    26521 aaaaaagtga tgaaatcggg cggagagaaa gcaagagaaa gtgcttctgc cacgttgaaa
    26581 gatgtgcgag aaataattgg tttcatgcat taaaattatg gccgaaaccc tgttttttga
    26641 aagtagtcca acagatcgac tgaagcgtta ccaagagcta aacactcaac tagctgcact
    26701 actatcggga gaaaaagatg aaatagcttg cttggctaat acgagtgcgg ctattactga
    26761 agcttttcaa tttcattggg ttggatttta tcgtgttgat gggaataatc tggttttagg
    26821 accatttcaa ggaccagttg cctgttctag aattgagcat ggtaaaggag tttgtggcaa
    26881 agcttggaag aataatcaaa ccgtttgcgt agaaaatgtg catgaatttc cgggacatat
    26941 tgcttgtagt ttattatcgc aatcggaaat tgtagtgccc ataagaaatc aaagcggtgt
    27001 tgtttgcgcc gtgttagata ttgatagttc tgaactagca gatttctctg aagtagatca
    27061 agctggatta gagatacttt gtaaaatgct tggggaacat ttgtattcgg catgagaaga
    27121 aagctaagcc aagtgtttga gtattccttt atgggactga tgttagcttc atgcgcaagt
    27181 cgtgttccgc ccaacggagg tgcgaaagac acggaagcac ctgtaatagt ttccagtgaa
    27241 ccggatagtg ctgctttaaa ttttcaagga aagaaagttc gatttacgtt tgatgaattc
    27301 attgatttaa aagatggagg aacgggaatc gttatatctc cgccattatc tattccacct
    27361 aagtatgtgg tgaagggtaa aacattagaa ataactttcg aagagaaatt agatagtgca
    27421 actacctatt ctattattct ggggaaatcg ataacagata tcagcgagaa caatccattc
    27481 ccaacaaaga catttgcttt tagtacaggt cctgttattg actccttaca aatgtcgggg
    27541 aatgtaacag atgcatacac gaataaggca gttaaagatg ctgttgtgat gttatatcca
    27601 actttagatg attcgcttcc ttataaaaaa cttcctcgct attttgctcg aaccgatgct
    27661 tcaggaaatt atcaaataga ggcgattcga gctggtgaat acttagcttt tgctttatcg
    27721 gatgctaatt cgaatttctt atatgatcaa ccagatgagc aaattggttt tttagcaaat
    27781 ccgattcgaa ttgattctaa taaaacgagt ggtattagtt ttagaatgag tccagaaaag
    27841 gctaaacgtc aaagacttat taaaaaggag tttgaacttc ccgggaaaat cacgttgaaa
    27901 tatgcattac cagttgagtc gctcgaacta aaggattata gtggtaatgc tcaaaccatt
    27961 tatcctgaat ttaatataga cagagattct attgtcctat ggttgaaacc agctcagacc
    28021 gatagcttga atttgatatg catttccaag tctttgggac aagaaaagat tgacacctta
    28081 aattatctac ccggtcaact cagacgaaaa gctacagtaa aaaagcgaaa taccagtaaa
    28141 gtagatacgt tgctgcattt tacaaacaac ttagaaaccg gcaagattcg acctacagag
    28201 aatttagagt tgatatctaa tcatccatca aaattgaaaa accgggatca agtttattgg
    28261 gtgatcggaa aagacacaac taaagctcag tttatagaat cggaggatcg ttttactttt
    28321 aaaacacagg taaaagttcc atctgaacta ggaaataaag cagttttagt gatgatgccg
    28381 ggtgcatttg aagatatttt ttcaatccaa aacgacacga caaaaacggc agtcaataga
    28441 tttgtagagg atgatttagg cttgttggat ttcagaatta aatcggcaga ttcaactagc
    28501 acatttattt tggaattatt taccagtaaa ggcaagaaac tctattcaaa acaagtaaaa
    28561 cctggaactc ctgttgtcat tgaaggatta gttcctggct cttatagtgc ccgaattatt
    28621 gaagatttag atcaagatgg ctcttggtcg aaaggacgat tttcagatcg aaaacagcct
    28681 gaacggattc gaccatattt aggtgaaata accattcggg caggatggga tttagagctt
    28741 gaatgggatc taaatgcaga aaaaaagtta tcagtcaaat agcctaatta cgcgtgaatt
    28801 ttaggattga caattctcct atcggattac gaatctcgat gaaatatagc cctgctgcag
    28861 cttgaaggtt catgctccac tggctattag aattgaagag cgtttcagaa tgtgtttgga
    28921 tacaacgtcc tccaatatct aaaattcgaa tagagtattt ccccgaaatt aactggttcc
    28981 cgcttaaaat aaactgccca tctgtagaag gatttggcca aagctgaaat gagttctcac
    29041 ttagattcaa acttgcttga aaaccttctg gaacatcatc tccggcaggg aaaacaacat
    29101 aatcaatcca tgcagcatct tcatttgacg aaatcatttc atctttagca tatacccaca
    29161 taaactcgtg caaacctgat gtaataggga attgctcttg agaccaatca accacacctg
    29221 accaagattg gatttcattt ccatccacat aaaattttag aaaatcatat ccttcctcag
    29281 aagaaacttt cttcataaaa cgaagtgtat ctgcaacaga aacgttccag ttgataatca
    29341 aactagatgt catattatta gaaatggtgc ccgacttaaa gctagttgcg ccttcatatt
    29401 tgatatcctg atcaatcgtc caaggtgatt catcggcatt ctgccaagca aaatgagtga
    29461 aatcgtttgt ttcagcatct tcaatcttag cacctacatg gataataaca tctttggtga
    29521 ttgaataatc tcctgccatc catgtataag tagcaggaat ataagaacca gcaagaacac
    29581 tagatgaaat ggtaagtgta tagttactag ctagttgatt tgcagcatca accgacaaaa
    29641 tcgattgcga tggttgatct actgtaaatt gattggttga acctgtaagt gttccgtttc
    29701 catcaagagt agcagcgtgt cccgcgttat tattgataaa ttcgatttgg atagattcac
    29761 ctgcatctgg taatccattg ccattacccg aaacttcagt gattacaaca gaagtaatat
    29821 gaagttctgg agcatttatc attactgtaa ttggctgaga ccattcttgt ccggcatcat
    29881 cggaataggt gatgataaaa tgagccaagg tttgatcctg aattgagctg cttacctgaa
    29941 actgaaaaca atcacttgac tgaattgaat ttcctgcatc cactaagcca atcggacatt
    30001 gattttgaag aatgatgata taggaatcgt tagatgttaa agttcccatt acatcgtttg
    30061 cagaaattcc acctacattt tgaatggtcg tattgaggta aatcgtttca ccaaagtcgg
    30121 ctagctgatt tccatttcca gaagcttcac taaaggagtg attactcact agaataaaag
    30181 catccgaagg attttcaatt tgaacagggc cttgatatgg gatacgatta aaagctgtaa
    30241 cagtaaccaa taaaggatcc atgagagtaa gtggttcaaa ttcaacccat gcttgtccac
    30301 cactcactaa agaagaaccg agtaaaattc cattctgagt gatagccact cgtgcatttt
    30361 cgacatcaac cgaaaccatg aaaccattcg aacctaatgc agttgttgca gcatgagtaa
    30421 cattcatggc aagaggaatg gaagtacgaa ccattaatga aggatcaccg aagacatgcc
    30481 aagtagcagt catttcatca ccggcatcgc catattcatc gttcattttg agacaaccat
    30541 ttaggcttag tccaccaaaa gtgcgagtag ttgtagttag aacatttccg gttagcagat
    30601 caaccatttc atcttgagca gccattggag gatcccaact ttgattgata gatgacataa
    30661 atgtagcaac gacaccagta agctgaccat ttcgagttgc tcttagccaa gcttcaccga
    30721 aacacgttcc attatcgaat tcaccattta cacagccaac agaccagata aatggtagca
    30781 atccattgtt ttggagttga ttgatttcgg agttggagaa tccgcttgtt ccaaagctaa
    30841 cagcagaacc atgaccagta taatttatca aacctacacc ttggttaaga atggtagaaa
    30901 catctgatgg ggaagggttt ccatccggat cgagttcccc ttgagagcca tcgtattgct
    30961 caaatgcttc tgcataagtg tacgaagtaa gcttatcgcg catcacccgt tcatgttccc
    31021 aatctaattc attgtcatcc cctggacctt gatctgaagc catcacgagc acttggtcga
    31081 gattggaagt aggaagaaga gctttctcgt attggacaat tttacttacc tgagtagcaa
    31141 catgagtgga attttcggca gagattcgac ctacaattac ttcgggatag gtatcatttc
    31201 caaggatgaa gccataggtt ggatccgatt tccctccaaa tgattgagga gaaggaattt
    31261 gactgatatc acccaccaaa agcaagtatg ttaaaccatt agatgtataa cgtgaatgaa
    31321 ccgcattggt gattcctgct acatttcctc cagcttcggc caatgtgatt acttcgacat
    31381 tcatacctga ttcacgtttc cattgaacaa gtggttcgag ttccgtttgg aaagccggat
    31441 cagtaataat caacatgttc gtttgctcgg taatcgcagt atagcgatcg gttccgaaat
    31501 tgacgaagtg attcgtatat agttcttggc ggacagaatt tactcgaatg tctgcatttc
    31561 ggtataaagg atttatagga gtatgaccag atacaggccg aagaattaca tcaatttgat
    31621 cggtaacatg taatgtatgc gtttgagcat tgtattgaaa tggatagaag agaactgttt
    31681 gtccgcggaa atcggaaagg atgtgtggat ttcgaagagc gacttgtgct tccgggaaaa
    31741 gagaattgct agaataaact tctccagcaa tacgtgaaat ggaattgggg tcgatggtac
    31801 gtttgatatt gcctttggaa ggagcgatat tgatattagt cagttcggtt tgatgagaac
    31861 taaggacttc ggcttgaagt gtagcaagat ctggaatgat gacagaagca gagaaaagag
    31921 gaagatctgg agctcctttt ctaaggattg gtgtacaacc ttcggcaacg acaatcgatt
    31981 gaggtccatt cggtgttgaa actgattgaa gtgcataatc acctaattga aaacgaagat
    32041 gtataggttg atttataccg tctccactta tccaagtaac gttgctttct tgtgcgtgaa
    32101 gcaatgaaac aaagcttgag aaaaggatgc aggtaaataa aaaggagagc ttcttcataa
    32161 aaaacaatgt tcagcaagat agaaaatact gagcatcaaa taaagaagcc agcttaaagc
    32221 tggcttaaaa ttatcgaaac gactgaaatt agtttcttcg actattcaag atagaaacat
    32281 aatacaacaa agtagcaatt gaaccaatgg cggcaaccac gtaagtcata gcagcccatt
    32341 gcaaagcatc tttcgcttta tcgtgttcgg ttccaacagt cattcgcgta ctttctagcc
    32401 aaattaaagc tctacgagaa gcatcaattt ctactggaag tgtgatgaga ctaaaaagcg
    32461 ttgtgagcgc gaaaagtaca actccagcaa gaagaagaaa cggaaatacg ttcaacaaaa
    32521 ggacaccagc aagtaaaatc cattgtacaa tattggaaga aaactgaaca gctggaacta
    32581 aagcagagcg catcgaaagc caagcatagc cttttgcatg ttgcaaagcg tgtccgcact
    32641 cgtgagccgc aactgcagca gaagagatac ttcttccatt atacacttca ggactcagat
    32701 ttactgtttt ttcgagcgga ttataatggt cagataaaaa gccttcaacc gaagtaacct
    32761 ttacatcagt aattccatta tcccgaagca ttttttcagc gatttccttt ccactaagtc
    32821 ctacttgtag gaaatcttct gaatactgtt tgaacttact ttttagtcgg tagctcacaa
    32881 accaagaagc tgccatgact aaaatcatta tcacatatat accacccatt tttttggttt
    32941 taagttgttg ctactggttt acaaacagta ttccgattga tttatttcag acagaaagtc
    33001 gaattgcctg tcagtcatgc ttaaatgaaa tgaaaatgtg tcaatgcagg aagaaataac
    33061 aaacccgagc gccattatgt cattcgacag aaaataaaaa tgccattatg gcttatttta
    33121 gtcgatggaa tgcaagttga aaaggggctg gaaacttaaa aacaataaca tcatgacact
    33181 tataaaattt aacaaaccag ctcaacgtcc tgttacacct atgttttcga atcttttcca
    33241 agatttattt gaccaagaac ttccaggatt tcgtttagga ggaaacacga atggtttacc
    33301 tgcggtgaat atttcagaaa cggaacaaaa cttccattta gaattgtcat cgccaggttt
    33361 tacgaaagaa gaaattgagc tttcgattga tcaggataca ttaatcgtaa gtggagaaaa
    33421 gaaatcggag aatgagcaaa cagagaaacg ttatagccgc aaagaattca gttatcagaa
    33481 tttcaagcgc agttttacgt tacccgaaaa tgtagatcaa gaaaagattt cggctcgctt
    33541 tgaacatggt attttactta ttgatttgcc gaaacgtgaa caagaagcta aaacggtaaa
    33601 aaagatatcg ttgaactaag attgggattg gttgattgag gggaaagcgt tggatattct
    33661 atccagcgct ttatttattt aaaataatcc cattatgttt gaggtatgac agaagaagca
    33721 tggtttctta ttgtaaatcc ccaagctggt ggtggaaaag ggcttaagaa aaaagcatcc
    33781 attatagctg ctttgcaaca tgctggactg agtcattttg tttacgaaac taaatgtccg
    33841 ggagatgctt ctacaagtgt ggaagaagcc gtatcaaagg gatatagaag atttgttgta
    33901 gctggaggag atgggacatt aaatgaggtg gcaaatggga tattaaatca gaaatatgtt
    33961 ccatccgacc aattattact agcacaaata ccagttggga cgggaaatga ttggagaaaa
    34021 agttggaaca ttcctaattc cattgaaggt tcagtagcct tattaaagaa cccaaaatca
    34081 gctaaacaag atgtggcatc gataactttt caagctaatg gaaaggaaga aaagttgtgg
    34141 tttatgaatg ttgcaggatg tggttttgat gcagaggtta ctttatcggc aaatgctgca
    34201 aaggcaaaag gcaagagtgg atttttcact tatatcggac aattggttaa aacattagca
    34261 agctatcaag aaccagaagt ccaatttcgg gttgataatc aaacgcattc agctcattta
    34321 ttttctttat tagcgggaat ctgcaagtat gccggaaatg caatgaagct tgttcctcaa
    34381 gcaaatccaa gtgacggatt atttcatttg acaattgctt caaaaatcag tcgattgaaa
    34441 gtcattttaa atttacctcg cctatttagt ggaagtttca cgcaactaaa agaggtaaaa
    34501 tctttaacct gcaccgaaat cgaaattcag acatcaggtt tacctattca ggcagatggt
    34561 gaatcaatcg gccatactcc agctatcttt gtcgttcatc ctctaaaact tcacatagta
    34621 atagcaccct gaatgttttc aaagaaagta acgaactggt ttcaagtatt tagctcagta
    34681 gaaaaggcga aagagcaggt tccgcttaac aaaacaatag ctgttgaatt aggtgacaag
    34741 cgcatctgtt tagcaagaac agaaaaaggt ttttttgctt tggatgaaaa atgtccccat
    34801 caaaatttac ctatcactca cggtggaatt tgtgaaaatg gaacaattgt atgtccgttt
    34861 caccgctatg catgggattt aaaaacaggt agagaagtgg aacgccgtga gggaaatatt
    34921 caattatacc cagttgaaga acgtgctgaa ggcttatttg tgggaattga aacgaagaaa
    34981 ggctggttta gttagcccaa taaataagct gaaattctga acgatcagta aattcaagaa
    35041 tagggcgagg atcggcaaga tttagattta ctattttcca tttcgaatta tcgtatagta
    35101 ttccaaagcc attcgccggg ttttcgactt tagaaaaatt ctgcttggga aatacctcat
    35161 cacccttttg atcaaagtaa ttgtagcctt cgcccgaacg tgaacatata gcaaaatcac
    35221 cataaaagtg accgatttct ttataggatc ccggaactaa aatttcgcaa tttgtgccta
    35281 atagtcccat ccattgttca gctccgatac ccgaatagaa aactagatta ggtacccgat
    35341 cggcttgaat ataatcgaac atctgcatgg gacaaagttg tccgttagca tccatcagcg
    35401 tatatccatt ttcgtttttt acaactgctt ttccatagca aaacatcgat gcttcttcga
    35461 aactgggata cgcaatgagc tgattcgaac gaagatctaa aaatccaact ttaccttttt
    35521 tgtaaactcg aataatagaa tctccatctt gaaattcgaa cttttcaaaa tcagggaaac
    35581 ggtttttcgc tttttcttct ttggttgaaa gcagatggat tttagctttt tctggatctg
    35641 aatcgggttt atgaatccat ttaccattag aagaaacgac tccatatttg gaatcctgtc
    35701 ggactaatat tttaccgtct ttaatttgaa taatctcatc aaaccgaggt ttaatacgca
    35761 catgaccgtt tgtatcagcc aatccccatt gagttccgaa tgcaaaaggg attagatgtt
    35821 cttgttcaga taggatttgg ttaacctgat tatcgcctaa taatttcagg gtagaagtat
    35881 ataatcgaaa gcccttctcg cccgtttttc gaacagcgat tctagttggc gatatcatta
    35941 cacacgaatc tacattttcg agctgtccaa aaggttgtac taactccttg agcgaatacg
    36001 gagcttgttt tcccacacga actaacgctt gggaaacaag aaaattcgtc ggccctaaaa
    36061 agcttaaaat aaagcagata aaggaaatgt atccaatact cggattcctt tttctgtttg
    36121 ttgcaaggtg gcgcattcga tggatttatc gtgttcgaaa aagagcactc tgttttctga
    36181 cacacaggta gcaagtacac ttgatttttc ctgcatcgtt aacaaaggac gtgtatcgta
    36241 tgccatcacc caagcaactg gaacgtgaac tggagatgga agtaagtcgg ccatgtacat
    36301 gattttgtgt ccgttgctcg acaaaacggg taacatcatc gattcggtat gtccgctaac
    36361 tttttcgacc gagaaacctg gaaatagctc gtttgattct agaaatttca gctgtccgga
    36421 ttcttggata ggaagaatat tttcttttag gaatgatgct ctttcacgag gattgggttc
    36481 ggttgcccat ttccaatgtt cttggttaga ccaatacacg gcatttttaa atgcaggacg
    36541 ataacctgaa cgagcatcat cccattcgat actaccgcca caatgatcaa aatgcaagtg
    36601 agtaagaaac acatcggtaa tatcatctcg atggaagcca gcttttttta aggaagcatc
    36661 gagcgaatca tcgccatgca aatcgtaatg agaaaagaat ttttcatcct gcttgtttcc
    36721 cattccgttg tcgattaata cgagccggtt tccttcttga acgagcaaac aacgcattgc
    36781 ccaagaacaa agattattgg catcggctgg ataactttta ctccataaag atttaggaac
    36841 aacgccaaac atagcaccac catcgagttt aaaatggccg gtattaatgg gaaataactg
    36901 catacttgag agattaaaaa tcgtaattaa ttgaccacag caggatgttc acaaattgag
    36961 aagaaaacac tatcctgcca aatgacaggg gctaaattta ccgtaaaatt tgaggaatga
    37021 aggttcattt gattgcaatt ggaggaagtg cgatgcataa tctggcaata gccatgtaca
    37081 aaaagggtta tgaagttagc ggatcggatg atgaggtgtt tgaaccttcc aaatcgaggt
    37141 tgtcagcttt agggctttta cccaaagaag aaggctggtt tcctgagaaa attcatgcag
    37201 gtttagatgc agttatctta ggaatgcatg cgagagttga taatcctgaa ttgattcgtg
    37261 cgcaggaatt aggcttgaag atttattctt atcctggata tatttatagt cagagtaccg
    37321 ataaaacccg tgtggtaata ggtggcagtc atggtaaaac atcgattact gccatgattt
    37381 tacacgcctt gcataccaat caaatggaca gtgattattt ggttggtgcg caattgaagg
    37441 gatttgatac gatggtgaga attacagcag aagcgcctcg cattttattg gaaggcgatg
    37501 aatatctggc ttctccaatc gagcgagttc ctaagtttca cctgtattat ccacatattg
    37561 ctttgttgag tggagtagct tgggatcaca ttaacgtatt cccaactttc gcgaattatg
    37621 tagagcaatt ccagattttc acggagaaga ttgagcccaa tggaacttta attttttacg
    37681 atggtgatga agaattggtg aagattgcgg cgcagtgtag accagatatt cgattgcaac
    37741 cttatcatga acatgcttcc gaaatacgtg atggtgttac ttatctagtt gcgggtgaag
    37801 agcgaattcc gttgaagatt ttcggtaaac acaatcttca gaatttgaat ggggcgatgt
    37861 tggtttgtga ggaattagga atgaagcggg tagattttta ccacgctatt caatcattta
    37921 cgggcgcagc caagcgactt gagttagtgt atggtggtga tgggacgagc attttcaaag
    37981 actttgcaca ttctccttca aaactaaaag cgactacaga tgcggtaaga gagcaattcc
    38041 ctaatcggaa actcattgct tgtatggagt tacatacgtt tagtagcttg aatgaggagt
    38101 ttctgttaga atacaagaat tccatggcga aagccgataa agcgttcatc tacttcaatc
    38161 cacacacagt agcacataaa aaattaaagc cgatcacacc tgaacaggta agagcggctt
    38221 ttggaagtga aaatgtgact gtttatgacg attcagctcg tttgttggaa gatttaaaac
    38281 aagaaaattg gtcgaattcc aatttgctga tgatgagttc aggaaatttt gacggattaa
    38341 atttcgagca gttaccgagt attttggggt taaagagcta agattttgaa ctaggtttag
    38401 cacttctctt ttccaacata gcattgcgct ttataatcat tcgcgtaatg gctcctgaat
    38461 aaaaaacacc aagaactaaa gtgccgatga acgtgaataa aacccatggc atttcaaatg
    38521 ggaagttatc gggattttga cctttacttt taaattcttc gatacgttcc ccagcaaatc
    38581 ttactttgaa catcgtatct aagtaaaaaa gatagtaata gatgaaaaga gaatagagca
    38641 ctgcaaataa agctgcaata gatgccccgc tcttaaacat ttctttaaaa ggaatgcttt
    38701 ccacatagct aagttttctc ctctcctcca ttcctctata catccctatt aacataaatg
    38761 cagttatagg taaaaatgga tatttaccaa gcgggccatc cacccaacct gttaaaagaa
    38821 gtaacgtttt ccaaattata gaaagcgcta aaatcagcaa agtccctcta gtttccggtt
    38881 tcatgcttag ccattcattg caatcaggaa ctcttcattg ctgattgtac cattcatttg
    38941 ttggcggatg aattccatcg cttcgactgt gttcatatcg cttaggtgat tacgcagaat
    39001 ccacatacgt tgaagggttt ctttatccac taaaagatca tcgcgacgag tagaagaagc
    39061 agttaaatcg atagcaggga acacacgttt gttggccagt ttacgatcca actgaagttc
    39121 catgttacct gttcctttga attcctcaaa gattacctcg tccattttag aaccagtatc
    39181 aatcaaggca gttgcgagaa tagttaatga accacctttt tcaatttttc gggcagcgcc
    39241 gaagaaacgt ttaggtttat gcagtgcgtt cgcatcaacc ccacccgaca atacttttcc
    39301 tgaagcaggt tgaaccgtgt tataagcacg agctaaacgg gtgatggaat cgagaagaat
    39361 aactacatct tgtccgcatt ctaccattcg ttttgctttt tcaagaacga tgttggcgat
    39421 ttttacgtga cgttctgcag gctcatcgaa cgtagaagaa ataacttctg ctttaacgct
    39481 acgagccata tcagtaacct cttctggacg ttcatcaatc aataaaatga tcaaataaac
    39541 ttccggatga ttatcagcaa tagcgtttgc aacttctttt aagagaactg ttttacccgt
    39601 tttaggttga gccacgataa gtccacgttg accttttcca ataggtgtaa ataggtccat
    39661 cacgcgagtc gacatggttc cttgtttgtg acctgtgagc tttaatttct cgtcagggaa
    39721 cataggagta aggaaatcga aaggaacacg atcacgcaca taagaagggt cgcgaccatt
    39781 gattttatcg attcgggtaa gcgggaaata tttttcgcct tcacgaggag cgcgaacacc
    39841 gccatcgacc gtatctcctg tttttagacc atagagttta acctgtgcaa ttgaaacata
    39901 cacgtcatcg ggtgatgcga ggtaattgta atcggatgaa cgcaagaatc cataaccatc
    39961 tggcatcatt tcgagcacgc cctccgttgg aacagtcaga tccagattca acaaaggatc
    40021 gataggcgta ttgctctcga catttggttg ttgttgattt ggattctgat taggattgga
    40081 gttctgattt gggttgcgtt gttgatgctg aggacgttgc atttgctgcc tctgctggtt
    40141 ttgatttgga ttctggtttt gccagtttcc gcgttgttgt tgtggatttt gaccttgatt
    40201 ctgctgctga ttctgatttt gttgttgatt ctgctgcgga tattgctgag caggcggtgt
    40261 ttgttgttgt tgcggagcag cagctggagg tgtttgtccg gctggtggct gataattagc
    40321 taggttatca gaacgtttat cgaaaagtga tctattagta cgatcacgac gttcgttac
//
LOCUS       DAOOQW010000004        37442 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_1274_length_37442_cov_7.289459, whole genome shotgun sequence.
ACCESSION   DAOOQW010000004 DAOOQW010000000
VERSION     DAOOQW010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 37442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37442
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1274_length_37442_cov_7.289459"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1822
                     /locus_tag="PK281_00635"
     CDS             <1..1822
                     /locus_tag="PK281_00635"
                     /inference="COORDINATES: protein motif:HMM:NF020678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptide-N-glycosidase F-related protein"
                     /protein_id="HOY47870.1"
                     /translation="TNVLIRTNPNVGSTTYKNWVVFPDASIGIHKVLMKLSFKCPTGE
                     NCGEWDYLNYIYLRRKGSVNSPSQDIELARYITPYGNTFNSVWSAEWEQDITDFEGLL
                     KDSIEIDYVHTGYETNVGRGWVINLSFQLIEGTPIRPYRDIHKLWNSSFIYGNANTPF
                     NTLVETKEIQLGASTESMRMRIMQSGHGAEPLELCSEFCPKMRTLSRNGEAYSNELVW
                     RDNCGLNPNYPQGGTWIYDRGNWCPGDLVYPSQYDMSTSASSTQTFSMSMEEFVSTSN
                     GNYMIESYLIEYGAPSFQTDASIEDILAPSTKYYYKRFNPICGRPKILIRNNGVQALT
                     SAQIEYGPAGGIKSSYTWNGNLAFMQSVEVELPANVIWGSSSGIFEAELKSVNGISDE
                     YSLNNRYQSSYIAPYVAQEQFVVNLLTNTMPQENSWRITDQSGNVLYSGDNFEANTEY
                     RDTVSLPEGCYEFIIEDSGKDGLSFWANEAGTGFIRFRRLGNNSILKSFNADFGTRVT
                     FPFTTGSTLGIESFTETEPVMQIFPNPSAGNSHLNIELNTPEDVQVQVFNSAGQVLVQ
                     LNPEKKSAHLLEMPDLVPGFYLVRVVAGTYEKTLRWLVVK"
     gene            complement(1823..4264)
                     /locus_tag="PK281_00640"
     CDS             complement(1823..4264)
                     /locus_tag="PK281_00640"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOY47871.1"
                     /translation="MRVLFISLISLLSLSLSSLAQNSITGTISDSNGQPIPGAIISIS
                     GTYLATSSNAQGKYNLQALKSEEVLVEVSMLGYVSSSKRITPSAKTTADFTLQRKVFL
                     ADEVVISATRAGDKSAMTFSSLSKKEIAEQNLGQDMPMLLNSQPSVVVNSDAGTGIGY
                     TGIRVRGSDPTRTNVTVNGIPMNDAESHGLFWVNMPDFASSVSSVQLQRGVGTSTNGA
                     GAFGATLNMQTNEFKGSPYASLTSSAGSFNTLRNTVEAGTGIINGFTFDLRLSKINSD
                     GYIDRAASDLKSYYLSAAWYKGKTSLRANVFSGIEKTYQAWYGVPQDSLISNPKYNPA
                     GSYVGTDGKEAFYSNQTDNYQQDHYQFFFNHQASKNVLLNAAVHYTRGRGYYEEYKED
                     QSFSNYGVEDLFVGQKQIIFENDTFYVPVDTISSTNLIRRRWLDNHFYGTVFSATFTP
                     TNRLRAIVGGGWNQYNGTHYGEVIWATYANAFPINYHYYQDTARKTDFNIYAKANYEI
                     STQLGVFLDIQYRTINYSFLGFNNSLVPFQQEITLRFLNPKGGVTLDINNQNRLFASV
                     SSGNREPVRDDYTNSSAKSRPTPEHLTDFEFGYRFKGKKIQAGLTGYYMNYINQLVLT
                     GAVNDVGAYNRINTPESFRAGIELELGWQILRKLNWQANLTLSQNKIRSFTEFVDNYD
                     TGLQDSILYTNTDISFSPSTIASSTLLYEPFRKLKIALISKYVGKQFLDNTQSDLRKL
                     DAFTTQSIRINWTPELIKAPGLELAFQINNLLDVKYNPNGYTYSGFSGNVRYDYNYYF
                     PQAGRNVMAMIKLKF"
     regulatory      complement(4319..4439)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(4478..4999)
                     /locus_tag="PK281_00645"
     CDS             complement(4478..4999)
                     /locus_tag="PK281_00645"
                     /inference="COORDINATES: protein motif:HMM:NF013231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOY47872.1"
                     /translation="MKKRLVPTFDLAFEDPSDSTGFLVWQVTNLWQRKMKLCLDPLGL
                     THVQFLLLNALATLNKTSSTAITQMMVANHANCDKMMTSKVLRTLEDRKLIMRKPHHL
                     DTRSRSLLITPRGMELIEKATPVFAAAEAEFFKSLKTKQKSIDKRMKKVIKINRKALK
                     SLADDASSLSSEE"
     gene            complement(5011..5343)
                     /locus_tag="PK281_00650"
     CDS             complement(5011..5343)
                     /locus_tag="PK281_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006988250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HOY47873.1"
                     /translation="MEADFSQFTALDLRVGTILEAKFFDKAIKPAYQLKINFGEAGIL
                     QSSAQLTLRYTVEDLIGMQIIAVINFPVKRIAGFRSDCLVLGATDEHGDVVLLRPEQP
                     VPDGWKIA"
     gene            complement(5345..6646)
                     /locus_tag="PK281_00655"
     CDS             complement(5345..6646)
                     /locus_tag="PK281_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY47874.1"
                     /translation="MRNWIRKPSAFILLLSACVLLLVSSNLNWPKDYWKGILRSDARG
                     YYAWLPAVFIYQDLSFEFQNTVENPQTDPRHQAVYRIPKENSITNKYFCGTALAELPF
                     FLTAHILTLLSSGNADGYSFLYQLFINLAGIFYALCGLYFLRRLLLQFKFSESTITWS
                     LIATLFATNLFYYSIIEPGMSHVFSFAFISAFLFYTHAWFRNPQTRLLLLASVCLGMI
                     ILIRPVNGFILLATPLLASSWDDFLTRIRLLLNSPKIVFPILITSSIIAIQPILWKIQ
                     TGNFFLDSYPGEDFHFLQPHLIDFLFSYKKGAFLYTPILALCLVGYLFLPKEKRPQLI
                     YGSAWGLALVYILSSWWNWWYGGSFSSRVLVEYLPVFAIGLALFLHQKRSWIKFVSRT
                     LVVVLLLVCQVQIYQYRYYQIHWEEMTVQRYWDVFLRPDLK"
     gene            complement(6646..7776)
                     /locus_tag="PK281_00660"
     CDS             complement(6646..7776)
                     /locus_tag="PK281_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015783087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HOY47875.1"
                     /translation="MEVVNKTDADVDLGAEQLNAYLPLLKGKRVALVANHTSLIGKTH
                     LVDTLRSSKVNLVKVFAPEHGFRGSADAGEKVKSATDAKTGLPLISLYGDNKKPKPEQ
                     LQDVDVVIYDIQDVGARFYTYISTLSLVMEACAEKGIPLIVLDRPNPNGHFTDGPVLD
                     PAFSSFVGMHPVPIVHGMTVGEYATMVNGEGWLKGKIKCTLTVITCKGWAHSNYYQIT
                     TAPSPNLQTMNAIYLYPTLCLFEGTNVSVGRGTPKPFEQIGVPGSTVGQYTFTPKSGP
                     GSKSPLYENKECKGFDLSGFGENFIRDTKMIYLFWLQSLYEANGKKPEYFNSFFDKLA
                     GTDTLRKQIIEGKSEAEIRKSWEPGLKAYREMRKKYLLYPES"
     gene            7902..9128
                     /locus_tag="PK281_00665"
     CDS             7902..9128
                     /locus_tag="PK281_00665"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOY47876.1"
                     /translation="MSTSAFIARRLLRSDAGSRLSTPIVRIAIAGIAIGISVMIVAIA
                     IVTGFQQEIRAKVIGFGSHIQISKYDTNRSFESTPISNTDSYLKEVGQLSGVQHIQSF
                     AIKAGIVKAGDAIEGVVYKGIGKDYESTFFQSHLKEGKMPVLPDSGRSNEVLISKTQA
                     QLLHLKVGDGLLMYFVQDPPKARKFTVSGIYDTGLGDNDFDKLYVIGDIRVVQQLNKW
                     SPDQVSGIEIQLEDFSKMDEMEQVVYQTIPNDLDAKSIRTRYPQLFSWLDLMDTNVYI
                     IIALMMIVGIINMVTSLLIMILERTQLIGILKALGADNRLVISLFLRQAATLIGRGLL
                     IGNVLGIGLCLLQLKTGMITLNQETYYLSVVPIQLHVSDILLLNAATFGICLVSLMLP
                     ALLVTRISPSRVMRFD"
     gene            complement(9097..10227)
                     /locus_tag="PK281_00670"
     CDS             complement(9097..10227)
                     /locus_tag="PK281_00670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY47877.1"
                     /translation="MSHSDSPRILVAPLDWGLGHAARCIPVIRELQNQGAQVIILGSP
                     RVLAYLNVHISNLEQIPSNDTGVKYSSWLPAWLKILLQANIIKRSIQDEYTNLQSLIK
                     IHQIDAIVSDNRYGLYAKSIPSILITHQVQPLTPRFLRFTQAFSNKRFLQLLKPFSEI
                     WIPDLDSNKISGRLAHVFSDSISVKNVGLLSRFGANLPPIKSSNSILAIISGPEPQRS
                     IFFQEIVRLQNAFFADTSIICENFPDKVSAASLENIRIILQPNDEQFIQEIERTQHLI
                     CRSGYSSIMDFIRLNRTALLIPTPGQTEQEYLSERCQKLGFTQATQRQLKDIKTNAEL
                     EVLFPSAKSPDLIISNEPEYSKAALKKAIESLLSRIASHAKD"
     gene            10275..10973
                     /locus_tag="PK281_00675"
     CDS             10275..10973
                     /locus_tag="PK281_00675"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOY47878.1"
                     /translation="MKAIVTGASSGIGHAIALKLAAAGWQLAVISRSEKRVDELLALL
                     GTLGMQEHIFMTGDLTHPGVVESFCSTVQERWGDVDLIVNNAGEYVPGDVGTLTLAQL
                     DEQMNVNFRSGFKLVQHFVPVMKLAGKGRIVFISSQVVREPRPFAAAYTLSKSLNDMY
                     ARLLGEELRESGVGVTRIVPGSVNTATWGDADVPRSQFVQPEEIAQLVSDICHMPNTT
                     WVEEIRIRPLDKNW"
     gene            10982..11644
                     /gene="trmB"
                     /locus_tag="PK281_00680"
     CDS             10982..11644
                     /gene="trmB"
                     /locus_tag="PK281_00680"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002677913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HOY47879.1"
                     /translation="MAKRKLRNFAELETFTNVIQHPYYKEQFDHPLKGKWHQEFFRNS
                     LPITLELGCGKGEYSVGLAIRHPERNYLGVDIKGNRIWRGAKTGIEDNIPNIAFLRTE
                     IEHLNNFFLPGEVDEIWITFPDPQPNKSKAHKRLTSAEFLDRYRKILRPGGLVHLKTD
                     SAFLFESTMAVIESQNLPVLRISRDIDTDFPGDELLQIRTYYELKFRQTGVPINYVAY
                     TL"
     gene            11641..11988
                     /locus_tag="PK281_00685"
     CDS             11641..11988
                     /locus_tag="PK281_00685"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004569584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HOY47880.1"
                     /translation="MNDPRDKEDFFEKVFAVVREIPAGRVTSYGAIARFLGTGGSART
                     VGWAMNLSHSAAIPVPAHRVVNRNGQLTGKHHFPNPDAMQEQLEAEGIVVKDDKIVGF
                     SKVFWDPQIELAL"
     gene            complement(11985..12260)
                     /locus_tag="PK281_00690"
     CDS             complement(11985..12260)
                     /locus_tag="PK281_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008636484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03643 family protein"
                     /protein_id="HOY47881.1"
                     /translation="MTASFQPIDIDRIIEMAWEDRTPFDAIFLQFGLREDDVKALMKR
                     ELHPNNWRKWRARVAGRHTKHVALRDMDVSRFKCKRQRSTGNRISKR"
     gene            complement(12297..12824)
                     /locus_tag="PK281_00695"
     CDS             complement(12297..12824)
                     /locus_tag="PK281_00695"
                     /inference="COORDINATES: protein motif:HMM:NF024911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOY47882.1"
                     /translation="MIRIAVTGPESTGKSTLAHQLAAHYSCPVVPEYAREYLMQLNRK
                     YAAYDVEAIARGQMDAENVLEKNHRLIICDTDLLVCRIWMEVVFGSCPDWLLKASRIP
                     RYTHTLLMNIDLPWVNDPLREHPTRRQELFDRYQIALKEDGRSFSIISGKDDKRFKTA
                     CEKVDLVLAKKKITS"
     gene            complement(12821..13390)
                     /gene="pnuC"
                     /locus_tag="PK281_00700"
     CDS             complement(12821..13390)
                     /gene="pnuC"
                     /locus_tag="PK281_00700"
                     /inference="COORDINATES: protein motif:HMM:NF016837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HOY47883.1"
                     /translation="MLGAQLFELSAFLLALLGVILTARGHIAGWAFGIFGSALYVPVF
                     YQSLLYAETGLQITYVLIGFFGWWNWNRLGKKASDRPVRNIPLNTAFALSILFLLASY
                     LLGLTLVHYSNTDVPFFDASMGVAGLIITWMMARKFIENWYCWVLVDLANSGLFIYKT
                     LHLTALLYIIMAALAVYGAFRWKKDLVLS"
     gene            13488..14990
                     /locus_tag="PK281_00705"
     CDS             13488..14990
                     /locus_tag="PK281_00705"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY47884.1"
                     /translation="MKFKFLLLFIWVFQFASAQRESYSWIYGNCDTTNNCQGVYGTAI
                     IKFNDDSIESVRRVEFPVPFGFLSSSISNAQGDLILATSRNYVFDSLGNTLLDFRTND
                     FNNLQLGYLRHSALFFKLKENTNDYYFINFKVAWIDSAENPLMNNRISRIYCSKIRVD
                     ENGYEVIKVDTLFRSDTILGSGIQACRHANGRDWWIFSNTFNQKQYLRGLLTPYGLSF
                     DIYDGPGPNVFQNSGQNHFSSDGTKLFHYITTSFRKMQIYDFDRCTGELSNFREMDFS
                     SYIPYPPYDFTPYVLSPDGSKIYMGKSNSDYTNYETIQVDIETGQVAVVADSVFVPLL
                     TPNLKWVISGYQDVFSTGIDDISAIKNPNNIGTACNRVILPNCLTVNGAISEPIEYAN
                     HILGPIDGSSCDTLGLDDETGIQKLKDYSFILFPNPGGNQLTIKTDLPLPVKLILRDS
                     QGKNVFEKKFNKNSFSIQQELVELNQGIYFVELMNVAQHKRLVRKWMKVE"
     gene            15290..15535
                     /locus_tag="PK281_00710"
     CDS             15290..15535
                     /locus_tag="PK281_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6364 family protein"
                     /protein_id="HOY47885.1"
                     /translation="MDAKLTLKLEQSVIEKAKDFAKKQQTSLSRLIENYLLSITNEEN
                     SEEKITPLVKSLSGIIDLPNDIDYKKGYADYLSNKYK"
     gene            15535..15945
                     /locus_tag="PK281_00715"
     CDS             15535..15945
                     /locus_tag="PK281_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004920723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HOY47886.1"
                     /translation="MERVFVDSDIILDLLGGREPFYKYSAELFSLADKGKVELHVSSL
                     SFANLNYILSKQFSAEQARKKLLKFKTLVTVLSVTDKIVELSLASDFKDFEDGLQYFT
                     AIENGIKLLLTRNLKDYKTAQISVMTAEQFLKGN"
     gene            complement(16031..16399)
                     /locus_tag="PK281_00720"
     CDS             complement(16031..16399)
                     /locus_tag="PK281_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY47887.1"
                     /translation="MKNLKPSLTKYFGIFMILGGIMHFINPSFYDAFIPDTLPKLAIN
                     IAGGIIELSLGIGLFIAKYKQKAALGILVLMILFLPLHIWDVFRESPAIGSHTAALIR
                     LPLQFVLIAWAWFISRNDVR"
     gene            complement(16438..17727)
                     /locus_tag="PK281_00725"
     CDS             complement(16438..17727)
                     /locus_tag="PK281_00725"
                     /inference="COORDINATES: protein motif:HMM:TIGR02765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003913 - DNA photolyase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASH family cryptochrome"
                     /protein_id="HOY47888.1"
                     /translation="MNVLLHWYRTDLRIQDHFALAQAADNGWSVLPVYFLPEEETFPL
                     SGLPRKGKFRKQFLYQTLQDLSNSWKQQGTELLIFSGKAEEILPQICSKYAIQQLSFT
                     ADCASEEMAQEIEVSHALKDLNVTIYKAFDDFLMHPEDLFCSIHEVPNVFTQFRQQCE
                     SNWHIRAPFKYGSPVGFSHLEKGILIEKLVSENRLIPDSRTAFPFSGGEHAALQRIED
                     YFFLSQQMLRYKETRNGLIGADYSTKFSPWLANGSVSPRTIYAQLKSLEEKLGANKST
                     YWLQFELLWRDYFRYMLLKFGAKLFFKNGLGIYHPPREMVQSETDFQNWINGNTGNNW
                     VDANMQELALTGFMSNRGRQNVASYLVHQLKLDWRLGAEYFESQLIDYDPANNWGNWA
                     YLAGVGNDPRQRVFNMQKQAEQYDTEGEFRNRWSRSE"
     gene            complement(17724..17864)
                     /locus_tag="PK281_00730"
     CDS             complement(17724..17864)
                     /locus_tag="PK281_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013073153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2256 domain-containing protein"
                     /protein_id="HOY47889.1"
                     /translation="MKGVKKANLPQKICPSCNRPLTWRKKWERSWDQVIYCSERCRNS
                     KS"
     gene            complement(17861..19180)
                     /locus_tag="PK281_00735"
     CDS             complement(17861..19180)
                     /locus_tag="PK281_00735"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015025176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HOY47890.1"
                     /translation="MKNKALFWFRRDLRLSDNAGLYHALRSGLEVQPIFIFDKNILDR
                     LEDDDDGRVSFIYQEISRLSEELQAIGSALNVYYSTPDEVFQQVAKEGNVQAVYTNHD
                     YEPYAKDRDESIRVLLNSQGIDFHTFKDQVIFEKGEVLKDNGTPYTVFTPYSKKWKEK
                     LSAFYLKSYPTEKYFAHFCKVQQKPIPTLEEMGFRPSAIKAFPRSVQTEIIRRYGTQR
                     DLPARLGTSRLSVHLRFGTVSIRQLASQAKELSDVWLNELIWRDFYHMILWHFPHAAV
                     SAFKPAYNHIEWRNNPEEFLKWCEGKTGYPIVDAGMRELNATGFMHNRVRMIVASFLT
                     KHLLIDWRWGEAWFARKLMDFDLAANNGGWQWAAGSGCDAAPYFRVFNPALQTERFDP
                     QAEYIRRWVPEFDDPFHYPRPIVDHKMARERALATYKAGLQVSGVEE"
     gene            complement(19188..19820)
                     /locus_tag="PK281_00740"
     CDS             complement(19188..19820)
                     /locus_tag="PK281_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HOY47891.1"
                     /translation="MNYQLDDILTLPERFRRNLINSVHGFKSVALIGTINPDGVTNLA
                     IFSQLFHIGANPPLVGMLVRPDSVGRHTLQNIRAIGWFTINHIQESFITAAHQTSARY
                     PDDVSEFDEVGLTPEFLPNILVPFVKESQVKYAVRKLEETYLINGTVLVTGKIEFLQL
                     PDHCLGEDGFIDLHQAGTITSAGLDAYYLTHPVSRLTYAKPGIIPQEIPR"
     gene            complement(19825..20535)
                     /locus_tag="PK281_00745"
     CDS             complement(19825..20535)
                     /locus_tag="PK281_00745"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOY47892.1"
                     /translation="MSEPKNYVILGGTSGIGEALVKLLAQEGHQVFVYARNIRNVQVS
                     SQIHFNEADVSQEFSLAGLPEVIHGLAYCPGSITLQPFHRIKTEQFQSDFQLNVMGAI
                     SAIQQCLSGLKASGSAAVVLFSTVAVQTGMSFHTSVAVSKGAVEGLTRALAAEYASSR
                     IRINCIAPSLTETPLAEKLLNTIEKKEAGAKRHPLGRIGQAEDSARVAQFLLNENEGW
                     ITGQILGVDGGMGSLRLI"
     gene            complement(20532..21224)
                     /gene="folE"
                     /locus_tag="PK281_00750"
     CDS             complement(20532..21224)
                     /gene="folE"
                     /locus_tag="PK281_00750"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019037262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HOY47893.1"
                     /translation="MKQNETLLNTTEPDISLEDLIGDEHISTAAETPLRADAFDIDDK
                     TKMIRIEEHFREIMNILGLDLTDDSLRGTPKRVAKMYVKEIFAGLDPKNKPEIALFEN
                     KYKYKEMLVEKDITFYSNCEHHFVPIIGKAHVAYISNGHVIGLSKINRIVQYFAHRPQ
                     VQERLTVQIANELKRVLKTDDVAVVIDAVHLCVSSRGVQDNGSKTVTSHFGGKFNQDE
                     TRKEFFTYVNLK"
     gene            complement(21184..21594)
                     /locus_tag="PK281_00755"
     CDS             complement(21184..21594)
                     /locus_tag="PK281_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008464474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HOY47894.1"
                     /translation="MNVTVIRKEHFNAAHRLHNPNWDDETNKRIFGKCNNSNYHGHNY
                     ELDVHVTGEIDSDTGYVIDMKILSDIIHEEVIERYDHRNFNLDIPEFATLNPTAENIA
                     VVIWNRIRERLNDRYALTVRLYETERNFVEYNGA"
     gene            21719..23224
                     /locus_tag="PK281_00760"
     CDS             21719..23224
                     /locus_tag="PK281_00760"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOY47895.1"
                     /translation="MSILNPIYLFKRASQANLPDSADVVILGSGLGSLSAAVVLAKQG
                     KRVVVLEQNYLPGGCVSSYFRHGVIFETGATTLVGLDEGMPLWEVIRQTGISIEAQRL
                     DLPMQVVLKDGTIVRRFNDPELWIAEAERVFGVQGQRAFWETCIRLSTQVWDISTRQL
                     SFPPASLTDILGCIKSARMKDLSALPYAFKTIQDLLFENGLDSNETFVAFVNEQLMIT
                     AQNTSQEVNALFGAASLCYTLVGNYYVPGGMIRLIAPFVEFIEQNRGDVLLKTPVSSI
                     KRVANQWEIHTGKGIIRSRAVISGLPVNNLVELVQDEKSIRKLQRKLMPIKSLNGAIQ
                     VSFVYRQRKEVSCLHQQIHLSESFEFEGSGSIFISKSDVGDRLRSELGEGILSVSMHY
                     RLGQKSNLDMERVKLVIQSILIREGYFEEDDIIRISASTPASWEDWTMRKGGFVGGYP
                     QRKNIKPWQMIQARVGSGLYVCGDSVYPGQGIPGVALSGLIAAKKLVQDGY"
     gene            23339..23851
                     /locus_tag="PK281_00765"
     CDS             23339..23851
                     /locus_tag="PK281_00765"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY47896.1"
                     /translation="MKKPLLLFALSAIPVLSWGQTQLSPQVFSSQGGDFSNAQFGVSY
                     TIGEMSSVSTISNGNFTLTQGFHQPDKYQIALVESIDASWNAEVYPNPADEQLTLKLS
                     SEKMLDLTLDLYDAEGRKVMASRKLNQVPGTQTYTVETSDLAAGAYLLRMTSVDGKHQ
                     RSFRITKMNS"
     gene            23871..26132
                     /locus_tag="PK281_00770"
     CDS             23871..26132
                     /locus_tag="PK281_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY47897.1"
                     /translation="MKRNFRSLLLTPALGLMLLAGTVQAQTPVPNGINYQAIARNSSG
                     SVFVNQTISVRISVLQGSATGPVQYSEKHTATTNSFGLFSFKIGGGTALTGTFEDITW
                     GTANQYVKVEVDPTGGTTYTTVGTNELLSVPYALYSESSGNGGTPGPQGPAGPAGATG
                     PAGPAGPAGPAGADGQDGQDGATGPAGATGPAGPAGPQGIQGPAGPGSGLNCWDTDGD
                     GVQDPSEDVNSDGFWNALDCRGAQGPAGPTGVAGPAGADGADGADGATGPAGATGPQG
                     PQGIAGVAGPQGPAGPAGATGPAGAQGATGATGATGLTGPAGATGATGLTGPAGPTGP
                     QGPIGLTGATGATGPAGPAGPTGLTGATGPAGPAGPAGATGATGPAGPQGPAGAGGTL
                     DQSYDFGGAGAGKTITADSGPVTINASGTGLTGIGLQINESGTATSGLAVVLSGTGTA
                     IQGASTNGANTFAAIQGTTNSTSVANSAILGLSTSAASGVSGQINAAGTAYAGVYGNN
                     LRTGAGVGVGGIGETGIGGESSSATGTGVFGIGNNIAFTSGTVLADGQGGAFNGFNFG
                     LYARNLNFAPGVDQAAIYTNDGDPSNTGTGVINCWTAADVHYKILGGGTVSTIVKDVN
                     DNAVVMHAPEAPEVLFEDFGSGQLVNGKAHITIDPTFAMNITVNEKHPLRVYIQVEGD
                     CKGVYITNKSVNGFDVVELAGGNSSTVFQWHIVANRSDKKEGSRVARYEDLRFESTPK
                     LQTLKNVDPLRKK"
     gene            complement(26334..27287)
                     /locus_tag="PK281_00775"
     CDS             complement(26334..27287)
                     /locus_tag="PK281_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY47898.1"
                     /translation="MIPFFRFTAISGILMAVCCFAACQSDVDHWKSAQTEWEQKDYLG
                     AIIELNTCISLDQQSDSIFLLRARCYQKIQNDSQAMGDFKQALLLNPNCDAARLDLAR
                     LQIIYTDTTNAGKSLRQLIISPTSRIRSDAWIEMGRMDYFSDRFPLAIQAMNQAIQAD
                     SSNHLAWYYRGLLFSRFFTPQGETRPATYPFLDFPAAINDFGQCIRLKPDFADAWYQR
                     AVVYFNQFDEQHGMPDINQAIRLEPKYSYYYSARAHQHSVSGRLQDALNDYNTSVNLN
                     ALDPEALLGRAEVWEKLGQKSNAEKDRHAAAIIALSSKNKD"
     gene            complement(27303..28730)
                     /locus_tag="PK281_00780"
     CDS             complement(27303..28730)
                     /locus_tag="PK281_00780"
                     /inference="COORDINATES: protein motif:HMM:NF016946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008488 - gamma-glutamyl carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0017187 - peptidyl-glutamic acid
                     carboxylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTTM domain-containing protein"
                     /protein_id="HOY47899.1"
                     /translation="MASVFAKIAYLPPLLKRMNMMEPEKDEKAGWFAQRTTFLFQPVA
                     LDPLIAFRVAFGVAMALWALYMLFSGNVYDFFIQPNYYFSYPGLSWIKPLSAPGMYGV
                     FILLFASAVMIGAGWFFRASTLIFTIAFAYVSLIDQANYLSYYYYVLLLSIMLSISPA
                     HRLFSIDLLRKPSLRVDFIPRWSILAFQLQVALVFIFAGMAKLNADWLFEGRPMNIWL
                     ENLSLNMGVDFPAWMLAGWFPVVISWFLILFDFIIPHFLLDKSTSTGAFRMVILIQLS
                     ALILFPAGFFPILTILSCTVFLPAEKIHRLISGISYLLYDLFSFKGEVFQPGGSYMLQ
                     YRKKRLFPSILLIFLSAQILIPVTLFLYWGNGRWTDAAFHFSWDIRLHEKEAQVQIWR
                     TNTENGQEEEVNINNYLAPHQQRRMAESPDMIRQFIHHLRYDGIDTSARDFEQVRALA
                     TISLNGGAPQPLLDNTSNEEQVEEN"
     gene            28777..29487
                     /locus_tag="PK281_00785"
     CDS             28777..29487
                     /locus_tag="PK281_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY47900.1"
                     /translation="MKRYFFLLLLVLPFSASIAQSAKMRLGFTAGPTLAWTSSNTNIT
                     TNFFTVGRAGVQLGVTNSLELGDHFFSSVSALYSLQSFGLKQTGIPSFGIDARFKSNN
                     LEFPLMFGTCGYLGSLRHREFVGAGLQINLSNVQTVKLSGDSSTVLTYTTQTNSLSNI
                     NPVFIAGFEVGSIFKSDAALYFGATFRYATTDVYQGQFTSNRFPVQLPTYNGTYLSFN
                     ITYYLPRYSYWFKREFIY"
     gene            complement(29488..30768)
                     /locus_tag="PK281_00790"
     CDS             complement(29488..30768)
                     /locus_tag="PK281_00790"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOY47901.1"
                     /translation="MNLVARFFCIILLLVLGSQVQAQTSRSELERKKKSLQKEIDLTN
                     KLIKETKKNKNLSLGQLKILNKKLDDRKRLINAIQQEVGMLNSTITTNGQKIQELQIE
                     LDRLKLSYGKLIRQAYLTRNQQSSLVLLFSAKDFQQAYKRLYYLRKYNNYRREQALLI
                     QASQDELNGRVKQLKTDLSTKRDLLGSEEREKKVLSEEKKEQEQTLTALQKKEKSLKK
                     DLDKKRADAKKLDGEIQRIIEREINRERDRAMAKSKSEAEAARKKAEASGKPVPAPVK
                     EPEMRVTSETRALSGKFEANKGRLPWPVDRGVITESFGTHPHPVLKGITTNNNGINIA
                     TPQGSNVKAIYDGEVTGVISIPGANMAVILKHGEYLTVYSNLASSSVSKGQKVHTGQA
                     IGVADKDDSGGKSEAHLEIWKGKVKLNPSEWISR"
     gene            complement(30752..31600)
                     /locus_tag="PK281_00795"
     CDS             complement(30752..31600)
                     /locus_tag="PK281_00795"
                     /inference="COORDINATES: protein motif:HMM:NF025490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4292 domain-containing protein"
                     /protein_id="HOY47902.1"
                     /translation="MKKAILPAIWLLVCVFTINSVACKSKKVAQVTPLPEQTTPKPTG
                     TFSSNPDSLSRALYAADVPLKWFSAKVSANTDIDKQTNSFSANLRLKKDSAIWMSISP
                     ALGIEVARALITPDSLKFINRINGTYFKGNYQYLNSLLQIEVNFKMVQSILLGNAYLH
                     YGVENYISDRENDLLVLSTLKKRRIRRESELDIPQILTQEIWFSALKNKIVQMEMQDY
                     RPVRKFTVSYKGFEDLEGFRVPNALSVHAQADKQVKIELEYSRITVNKELNLPFNIPE
                     NYEPSR"
     gene            complement(31601..33343)
                     /locus_tag="PK281_00800"
     CDS             complement(31601..33343)
                     /locus_tag="PK281_00800"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOY47903.1"
                     /translation="MRPINLILFSCIIIGSLSSCKNKQVASADKNPSTASALTEKERL
                     DFEVLFFDAEKEKLLGNFSQAQTKFQQAIRIDPRAAAAHFELSQVYLQTGNKDLAEIS
                     GKNAVRFDEKNKWYKLALADIYARNDKYAEVVPLMESLHKKEPNNEDYLYGLGAALVQ
                     TGKFDEAAKVYNKLEALVGINEELILQKKNLYMRLGKTDKAIAEINRLMAAFPEEVGY
                     RGFLAEIYEATNQPEKALAEYNEILRLDPENPNVRFSLAEYYRSQGDKEKSYQELKKA
                     FANPESSLELKMQVLSSYFEITAQYPELKYQAMELCRELVAAHPEEPQGHAVYGDFLL
                     REDKLNEAREQYMLVLAADKSRYNVWNQILLIDSELKNYQSMFDLSKEAIELFPYQPS
                     LFLYNGIAAMQLKQYDKAIESLKGGADVTIGNSALSSQFYASMGDSYNSMTKFRESND
                     AYEKALKYDAANTYVLNNYAYYLSLRKENLPRAKELAEKCNELQPGNASFQDTYAWIL
                     FQMNDLENASIWIDKAIENGGIRSGTIMEHKGDILIKQGKTTDALDFWNRAKELGGTS
                     DKLEQKISQKKYSE"
     gene            complement(33353..34363)
                     /locus_tag="PK281_00805"
     CDS             complement(33353..34363)
                     /locus_tag="PK281_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOY47904.1"
                     /translation="MRIIIPMAGMGKRMRPHTLVTPKPLIEFAGKPMVHHIVEELAKT
                     CHEPIEEIAFVIGNFGKEAEKSLIAVAEKVGAKGSIYYQEEALGTAHAVLCAAESMQG
                     KLIIAFADTLFKANFVLDESKDAVIWVQQVEDPRPFGVVKVNEEGNITDFVEKPAEYV
                     SDLAIIGIYYFRDGENLRAELQYLIDNDLKEKGEYQITNALENMKQKGLKFAPGKVIE
                     WLDCGNKNATVYTHSRWLELKKGEQLIDASLVKENALVIEPCYIGKGVKIIGSVVGPH
                     VSIADGSLIENSVIQNSIIQKNTRIQNSVIANSMIGNSVEIMNQKLDLSVGDYTVFST
                     GN"
     gene            complement(34388..34822)
                     /gene="dut"
                     /locus_tag="PK281_00810"
     CDS             complement(34388..34822)
                     /gene="dut"
                     /locus_tag="PK281_00810"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014032722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HOY47905.1"
                     /translation="MKIRIINQSAHALPEYGTEHAAGCDLRANLTEPIVLAPLGRALI
                     PTGLFLEIPVGMEGQVRPRSGLAAKHGVTVLNSPGTIDADYRGEIKVILVNLSDTAFT
                     INNGERIAQLVVAKHERAEWEESSSLADSERGAGGFGHTGVH"
     gene            complement(34837..36321)
                     /locus_tag="PK281_00815"
     CDS             complement(34837..36321)
                     /locus_tag="PK281_00815"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HOY47906.1"
                     /translation="MNPLRKLAGQTAIYGLSSIVGRLLNYLLVPLHTRVFLPEAYGVV
                     TEMYSYVAFLMIVLTYGFETSFFRFASNQEDRPRIYATSLISLLVSSLGFFILCWVFV
                     GPIADYIQYPEYPEYIVWFALILATDAATSIPFAKLRLENRPLKFAILKLVNIGVNVL
                     INVFFLMLCPWLLENGSTSIQDAVRDIYDPNMGVGYVFIANLVASLVTFLLLIPDMWK
                     MSWKFDSELWRRMMRYGFPLLIGGLAGITNEMFSRMSMKYQLPKSIAMHDLGVFGACY
                     KVAILMTLFIQTFRFAAEPFFFTQAREKNAKKLYAEVMHWFVIACSLIYLGVLIFMNI
                     VKEFIGEAYHEGLVVVPILLMAQLFLGVFFNLSIWFKLNDRTYIGAWLAIFGSVLTLI
                     LNYLWVPEFGYVGASWATFVVYLTMLIASYLLGQKFYPIEYDLRRVIGYPVSVALMVW
                     LYTKYLPMEGAISWVLKCLILLAFVGAVWLLEVRKKTVISSPST"
     gene            complement(36419..37198)
                     /locus_tag="PK281_00820"
     CDS             complement(36419..37198)
                     /locus_tag="PK281_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOY47907.1"
                     /translation="MSFQNILVSVNEGIQTITVNRPDKLNALNYQTIQEIGQAVAQGN
                     KNPDVFGMILTGSGEKAFVAGADISEFASYSLIEAEKLSRDGGDVFTGIERSAKPVIA
                     AVNGFALGGGCELAMSCQIRVAAENARFGQPEVNLGLIPGYAGTQRLVQLIGKGKALE
                     LLMTADMIGAEEAHRLGLVNHVVSQAELINKATEILKKIGTKAPLAIAAVIRCVNAHY
                     QYDENGFDTEIREFAGCFGTEDFKEGTEAFLGKRKAEFKGK"
     gene            complement(37195..>37442)
                     /locus_tag="PK281_00825"
     CDS             complement(37195..>37442)
                     /locus_tag="PK281_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010230827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY47908.1"
                     /translation="DTHTNCANVDCNLLFIQCENCQKEFNGCCSAECMEIIALPEETQ
                     KELRKNKNYQYSNMLYRKGRLRPSGPQLQQYTLKSKS"
BASE COUNT        10721 a         7391 c         8250 g        11080 t
ORIGIN      
        1 taccaatgtg ctaatacgaa cgaatcccaa tgtgggttct acgacgtata aaaattgggt
       61 tgtttttcct gatgcttcaa ttggtattca taaagtactg atgaaacttt cgttcaaatg
      121 tccgacaggg gagaattgcg gagaatggga ttatctaaat tatatctatc tccgaagaaa
      181 aggaagtgtg aacagccctt ctcaagacat tgagctggca cgttatatca ctccatatgg
      241 aaatacattt aattctgttt ggtcggccga atgggaacag gacattactg actttgaagg
      301 actactaaaa gattctatcg aaatcgatta tgtgcatact ggatatgaaa cgaatgttgg
      361 acgaggctgg gttataaatt tgtcatttca attaatagaa ggaactccca ttagaccata
      421 tcgtgacatt cataaattat ggaacagctc atttatttat ggaaatgcga atacaccttt
      481 taacacactt gttgagacta aggaaattca attaggagct tcaacagaat ccatgcggat
      541 gcgcattatg caaagtggtc atggtgctga gccattagaa ttatgctcgg aattttgccc
      601 taaaatgaga acgctttctc gcaatggaga agcatattcc aatgaattag tttggagaga
      661 taactgtgga ctaaatccca actatcctca aggaggaaca tggatttacg acagaggaaa
      721 ttggtgtccg ggcgatttag tatatcctag tcaatatgac atgagtactt cggcgagctc
      781 aacacaaaca ttttcgatga gtatggaaga gtttgtttct acctcaaatg ggaattatat
      841 gattgagtcc tatttaattg agtatggagc accaagtttc caaactgatg catcgatcga
      901 ggatatttta gctcctagca cgaagtatta ttataaacgc ttcaatccaa tttgcggaag
      961 acccaaaatt ttgattcgaa acaatggagt tcaagccttg acttcggctc aaatagaata
     1021 tggacctgcg ggaggaataa aaagcagtta tacttggaat ggaaatcttg cttttatgca
     1081 atcggttgag gtagaattac ctgcaaatgt aatttggggg agttcttcag gaatattcga
     1141 ggctgaattg aaatcggtga atgggatttc ggatgaatat tcattgaata atcgctacca
     1201 atcgagttat atcgcgcctt atgtggcaca ggagcaattt gtggtgaatt tgttaactaa
     1261 tacgatgcct caagaaaact cctggaggat tactgatcag tcgggaaatg tgctctacag
     1321 cggagataat ttcgaagcga ataccgaata ccgtgataca gttagtttgc ctgaaggctg
     1381 ctacgaattt atcattgaag attcaggaaa ggatggatta agcttctggg caaatgaagc
     1441 cggaactggt tttatccgat ttagaagact tggaaataat tctatcctca aatcattcaa
     1501 tgcagatttt ggaacacgtg taacatttcc atttaccacc ggatcaacat tgggaataga
     1561 atcgtttact gaaacagagc cagtcatgca gatttttccc aatccatcag ctggaaattc
     1621 acatttaaac attgaattga atacgcccga agatgttcag gtgcaggtat ttaattcggc
     1681 tggacaagtt ttggttcaat taaatccgga gaagaaatca gcacatttat tagagatgcc
     1741 tgacctcgtt cctggttttt acttagttcg tgttgttgcc ggaacgtatg aaaaaacctt
     1801 gcgttggttg gtcgtgaagt aattagaatt tgagttttat catcgccatc acgtttctgc
     1861 ctgcttgagg aaagtagtaa ttgtagtcgt aacgcacatt tccggagaat cctgaatagg
     1921 tataaccatt cgggttgtat ttcacatcga gcaggttgtt gatttgaaaa gccaattcca
     1981 aaccgggtgc tttaatgagt tcgggtgtcc agtttattcg gatggattgt gtggtaaagg
     2041 catccaattt tcgcaaatct gattgagtat tatcgaggaa ttgtttccct acgtatttgc
     2101 tgattagcgc aattttcagt ttgcggaatg gttcgtacaa gagggtagaa gatgcaatag
     2161 ttgaaggaga gaatgaaata tcggtattcg tataaaggat gctgtcttga agtccggtat
     2221 cgtaattatc tacaaactca gtgaacgagc ggattttatt ttggctgagg gttagattcg
     2281 cttgccaatt gagtttgcga agaatttgcc aaccaagttc taattcaatt ccggcgcgga
     2341 aactttcagg tgtgttgata cgattataag caccaacatc attcacggct ccggttagca
     2401 ccaattgatt gatgtaattc atgtaatatc ctgttaatcc agcttgtatc tttttccctt
     2461 taaaacgata tccaaattcg aagtcagtta gatgttctgg tgtagggcga ctttttgccg
     2521 aagaattggt atagtcatcc cgaacgggtt ctctattacc agatgaaaca gaagcaaaga
     2581 gtcggttttg attattaata tccaaagtaa caccgccttt aggatttaaa aaccggagtg
     2641 ttatttcctg ttggaaaggc actaatgaat tattgaaccc taaaaacgag tagttaatag
     2701 tacggtattg aatatcgagg aaaacgccaa gctgggtact aatctcataa tttgcttttg
     2761 cgtatatatt gaaatcagtt ttgcgagcag tgtcttggta gtaatggtag ttaatcggga
     2821 aagcattggc atacgtagcc caaattactt ccccataatg tgttccattg tattgattcc
     2881 atcctccacc aacaatggct cttagtcgat tggtaggagt gaaagtggca gagaaaacag
     2941 ttccgtaaaa atgattgtcg agccaacgac gacgaatgag gttggtactt gaaatggtat
     3001 cgacaggaac ataaaaagtg tcgttttcaa aaataatttg cttttgacct acaaataaat
     3061 cctccacacc ataattcgaa aacgactgat cttccttgta ttcttcgtaa tatcctcgtc
     3121 cacgcgtata atggacagct gcatttaaca gtacattttt acttgcttgg tggttgaaga
     3181 aaaactggta atgatcctgc tgataattat cggtttgatt ggaatagaag gcttctttcc
     3241 catccgttcc cacatagctt ccagcaggat tatactttgg gttgcttatc aacgaatctt
     3301 gtggaacacc ataccaagcc tgatacgttt tctcgattcc cgaaaacaca tttgcccgca
     3361 agctagtttt acccttatac caagcggcac ttaggtaata ggatttaaga tcggacgctg
     3421 cacggtcgat atatccatca ctattgattt tagagaggcg caaatcgaag gtgaacccgt
     3481 tgatgattcc tgtcccagct tcaacagtgt tccgtaaggt gttaaaggat ccggcactgc
     3541 tggttagtga agcgtatggg ctccctttaa attcattcgt ttgcatattt aacgttgccc
     3601 cgaaagcacc agctccattg gtggatgttc ctacgccacg ctgcaattgc acactgctaa
     3661 cagatgaagc aaaatcgggc atgttcaccc aaaataatcc atgcgattcg gcatcattca
     3721 ttgggatgcc attcacggta acgtttgtac gagtaggatc acttccgcga acgcgaatac
     3781 ccgtataacc aatgcctgtt cctgcatctg aattcaccac aaccgagggc tgactattca
     3841 aaagcatagg catatcctga cctaagtttt gttctgctat ttctttctta gaaagactgc
     3901 taaacgtcat ggcagattta tcacctgcac gggttgcgct aatcacaact tcgtcggcta
     3961 gaaagacttt gcgttgaagc gtaaaatcgg cggtagtttt ggctgatgga gtgattcgtt
     4021 tactactaga cacatagcct aacatactca cttctactag tacttcttcg gatttgagcg
     4081 cttgtagatt gtatttccct tgggcgtttg atgaagtagc caaataggtt cccgaaatgc
     4141 tgatgatagc acccggaatg ggttgtccat tgctgtcgga aatggttcct gtaatggaat
     4201 tttgggctaa actgcttaag gaaaggctaa gcaatgagat cagagaaatg aataaaacgc
     4261 gcatcgctgt tgtattaaac aaacaacgga tcgcaggggt cgacgaatcc gactagtagt
     4321 aaaacaatac catcctccct acgccggcat tacccggatc aggttcgatc gctcaaaagg
     4381 ctagagcgat aagggtataa tctcagccat gcttatgcac agcacccctt gtaaacttct
     4441 gcaaatcaac gtaaggaagt tgaatcctgc aaataaatta ttcttcgcta cttaaggaag
     4501 aagcgtcatc agccagtgat ttcaaggctt tgcgatttat tttgatgact ttcttcatgc
     4561 gtttatcgat cgatttctgt ttagtcttaa gacttttgaa gaattcggcc tcagctgctg
     4621 caaaaacagg tgtagctttt tcgatcaatt ccattccgcg aggcgtgata agcaaggaac
     4681 gagagcgagt atctaggtga tgcggtttgc gcataatgag ttttcggtct tcgagtgtac
     4741 gaaggacttt tgacgtcatc attttatcgc aattagcatg attagccacc atcatttgag
     4801 tgatagcggt tgatgatgtt ttattgagtg ttgctaaggc gtttaagagc agaaattgaa
     4861 catgcgtgag cccaagtgga tcgagacaca acttcatttt gcgttgccaa aggtttgtta
     4921 cctgccaaac aagaaagccg gtactatcgg aaggatcttc aaatgcaagg tcaaaagttg
     4981 ggaccagtcg tttcttcatg ttttaggtat tcaagctatt ttccaaccat cgggaacagg
     5041 ttgttcggga cgaagcagca ccacatcccc atgttcatcg gtagcgccca aaactaagca
     5101 atcggagcgg aatcccgcga ttcgttttac cggaaaattg ataacagcaa taatctgcat
     5161 accgataaga tcttctaccg tgtaacgaag tgtgagctga gcactgcttt gtaatattcc
     5221 ggcttcgcca aagttaattt tcagttgata agctggtttg atggctttat cgaaaaattt
     5281 tgcttcgaga atggttccca ctcgcaaatc gagcgcagta aactgagaga aatctgcttc
     5341 catcttattt caaatcgggg cgaagaaata catcccaata ccgctgaact gtcatttctt
     5401 cccagtgaat ttggtaataa cgatattgat aaatctgaac ctgacaaaca agcagcaaca
     5461 ccacgactag agtgcgcgaa acgaatttta tccagcttct tttttggtgt agaaaaagtg
     5521 ccaatccgat tgcgaatacg ggtaaatatt ctaccagtac gcgtgatgaa aaacttcctc
     5581 cataccacca attccaccaa gaagataaga tgtaaacgag cgccaagccc caagcggaac
     5641 catatattag ttgaggtctt ttttctttag gtagaaacag gtatccgact aaacagaggg
     5701 ctaatattgg tgtatagaga aatgctcctt ttttatagct aaataaaaag tcgattaagt
     5761 gtggttgaag aaaatgaaag tcctcaccgg gataagaatc gaggaagaaa ttacctgttt
     5821 gaatcttcca gagaataggc tgaatggcta tgatagaaga tgtaatcaga attgggaaaa
     5881 cgatttttgg ggaattcagc aacaatcgta ttcgtgtcaa aaaatcatcc caagaacttg
     5941 ctagcaaagg tgttgcaaga aggatgaatc catttactgg acgaatcaga ataatcattc
     6001 ctaaacatac agaagctaat aggagcaagc gagtttgggg atttctaaac caagcgtgcg
     6061 tataaaacag gaatgcagag ataaaagcaa acgagaatac atgcgacatt cccggttcta
     6121 taatgctgta atagaacagg tttgttgcaa aaagtgtggc gatgagcgac caagttattg
     6181 tgctttcgga gaacttgaat tggagtagca atcggcgaag aaaatacaat ccgcagagtg
     6241 cataaaatat tcctgccagg ttgatgaata actgatagag aaacgaatag ccatcagcat
     6301 ttccgctgct taataatgtt aaaatgtgag ctgttaagaa gaatggtagt tcggccaatg
     6361 ccgttccgca gaagtattta ttcgttatag aattttcctt tggaatgcga taaacagcct
     6421 gatgacgcgg atcagtttga ggattctcga cagtattttg gaattcgaaa cttaagtctt
     6481 gatagatgaa aacagcggga agccaagcat aatatccacg tgcatcggag cgaagaattc
     6541 ctttccaata atcttttggc cagttgaggt tagaagaaac caagagcaaa acgcaagcgc
     6601 tcagcagcag aataaaagct gaaggtttac gaatccaatt acgcatcaag actcaggata
     6661 aagcaggtat ttttttcgca tctcgcgata tgctttcaaa cccggctccc agctttttcg
     6721 gatttcagct tcagatttac cttctataat ttgttttctc agcgtatccg tcccagctag
     6781 cttatcaaag aaactgttga aatactccgg cttcttgcca ttagcttcat ataaactctg
     6841 aagccaaaaa aggtagatca ttttcgtatc gcgaatgaaa ttctcaccaa atccacttaa
     6901 gtcgaaccct ttgcattctt tgttttcgta gagtggcgat ttgcttcctg gtcctgattt
     6961 gggagtaaat gtatactgtc ctacagtcga tcccgggacc ccgatttgtt cgaaaggttt
     7021 cggagtacct cttcccacgc ttacgttagt tccttcgaac aaacacaaag ttggatacaa
     7081 gtaaatggca ttcatcgttt gcaaattggg cgatggcgca gtagtaattt ggtaatagtt
     7141 gctgtgtgcc cagcctttgc atgtaataac tgtcagcgtg catttgattt ttcctttcag
     7201 ccaaccttca ccatttacca tggtagcata ttcacccact gtcataccgt gaacgattgg
     7261 aaccggatgc ataccaacaa aagatgaaaa tgctggatcg agcacaggac catcagtaaa
     7321 atgtccgttg ggatttggtc ggtcgagcac aattaatgga attccttttt cggcgcaggc
     7381 ttccattacg agtgataacg ttgagatgta agtgtagaaa cgcgctccca catcttggat
     7441 atcataaatc accacatcta catcttgtaa ttgctcgggc tttggttttt tattgtcgcc
     7501 ataaagcgaa atgagcggaa gtccggtttt agcatctgtt gctgatttaa ctttttctcc
     7561 tgcatcagca gagccacgaa agccatgttc gggggcaaaa actttgacca gatttacttt
     7621 gctggaacgt aaagtatcta ccagatgtgt ttttccaatt aatgaagtgt ggttggctac
     7681 caatgcaact cgttttcctt tgagtaatgg caggtaggca ttcaattgct cagcgcctaa
     7741 atccacatca gcatctgttt tattgaccac ttccacgcgg gagaatattt gtggcaagat
     7801 gttatgtgga aacaggattg cgcaaaggag catcaggctg aaggccttaa aattcctaac
     7861 ttcgggcact cgttttatca gattcattca gtttatcagg attgagcacc tccgccttta
     7921 ttgcccgtcg ccttttacgt tcggatgccg gtagccgttt gtcaacaccc attgttcgta
     7981 ttgccattgc aggcattgcg atcggaataa gtgtgatgat tgtggctata gcaatcgtga
     8041 ctgggtttca gcaagagatc agggcgaaag ttattggttt cgggtcgcat attcaaatca
     8101 gcaaatacga caccaaccgt tccttcgaaa gtactccaat tagcaatact gacagctatt
     8161 tgaaagaagt tgggcaactg tcgggagttc aacacatcca gagttttgct attaaagcag
     8221 gcattgtaaa agcgggagat gcgatagaag gggttgttta caaaggcatc gggaaagatt
     8281 atgaatctac atttttccaa tcacatttga aagaaggaaa gatgccagta ttgcctgatt
     8341 cggggcgttc gaatgaggta ttaatttcga aaacgcaagc tcaattattg catttgaaag
     8401 tgggtgatgg cttgttaatg tattttgttc aagatcctcc caaagctcga aaattcaccg
     8461 tttcgggaat ttatgatacc ggattgggcg ataatgattt tgacaaactt tatgtaattg
     8521 gcgatatacg tgttgttcag cagttgaata aatggtcgcc tgatcaagtg agcggaattg
     8581 aaattcagtt ggaagatttt tccaaaatgg atgaaatgga acaggtagtt tatcaaacca
     8641 ttccaaacga tttggatgca aaaagtattc gaacgcgcta tccgcagttg tttagttggc
     8701 tcgatttgat ggatacaaac gtgtacatca ttatcgcact catgatgata gttggaatca
     8761 ttaacatggt tacttccttg ctcatcatga ttcttgaacg cacgcagcta attggaattt
     8821 tgaaagcttt gggagccgat aatcgactcg ttatttcgtt gtttctaaga caagctgcca
     8881 ccttgattgg acgaggtttg cttataggaa atgtgctagg aatcggattg tgtttgctac
     8941 aattaaaaac cggaatgatt acgcttaatc aggaaaccta ttatttatcg gttgttccca
     9001 ttcaactgca tgtaagcgac atacttttat taaacgccgc aacctttggg atttgtttgg
     9061 tttcgctgat gttgccagct ttgttggtga cacgaattag tccttcgcgt gtgatgcgat
     9121 tcgactaagc aaggattcaa ttgctttttt aagtgcggct ttactgtatt ccggctcgtt
     9181 tgaaataatc aaatcaggag atttagctga tgggaaaagt acttccaatt cggcattcgt
     9241 tttaatgtct ttcagttgtc tttgggtagc ttgagtaaag cccaatttct gacacctttc
     9301 tgacaagtat tcttgttcgg tttgtccggg tgttgggatg agtaatgcag ttcgatttaa
     9361 gcggatgaaa tccataatac tggaataacc tgaacgacaa atgagatgct gcgtacgttc
     9421 gatttcttgg ataaattgtt catcgtttgg ctgaagaatt atccggatat tttctaaaga
     9481 agccgcactt accttatcgg gaaaattctc acatataata gaagtatcgg cgaaaaatgc
     9541 attttgtaaa cgcacaattt cttggaagaa gatacttcgt tgaggttcgg gaccagagat
     9601 aattgccaaa atactattgg atgattttat tggcggaaga ttcgctccaa accgacttaa
     9661 tagtcccaca ttttttactg aaatcgaatc agaaaaaaca tgagctaatc gaccactgat
     9721 tttgtttgaa tctagatcag gaatccagat ttcggagaag ggtttaagaa gttgtaaaaa
     9781 tcgtttattg ctgaatgctt gtgtgaaacg aagaaaacga ggagttagcg gttgaacttg
     9841 gtgggtgatg aggatactcg gaatggattt ggcatacaac ccataacggt tatcggaaac
     9901 gattgcatcg atttgatgaa ttttaatcag cgattgtaag ttcgtatatt catcttgtat
     9961 ggaacgcttt attatgttgg cttgtagcag tatttttaac caagcaggaa gccaggatga
    10021 atacttcaca ccagtatcat ttgatggaat ttgttccaga ttagaaatgt gcacatttag
    10081 gtaagctaag acgcgaggag aacctaagat aattacctgt gctccttgat tctgaagttc
    10141 tcgtatgact ggaatgcatc gagcagcgtg acccaagccc caatcgagag gagcaacaag
    10201 aatacgtgga gagtctgaat gcgacatgtt gcaaaataac cggtatttcc tccacaaacc
    10261 gctacttttg cagaatgaaa gcaatcgtta caggagcaag tagtggaatt gggcatgcta
    10321 tcgcgttgaa attagctgca gcaggctggc aattagctgt catttcgaga agtgagaaac
    10381 gcgtggatga actactggct ttgttaggaa cacttggcat gcaggaacat attttcatga
    10441 cgggcgactt aacgcatccc ggtgtagttg aatcattttg ctccaccgta caagaacgtt
    10501 ggggcgatgt agatttaatc gtgaataatg ctggagaata tgttccgggt gatgtgggaa
    10561 cattaacatt ggctcagtta gatgagcaga tgaatgtcaa cttccgaagt ggttttaagc
    10621 tggtgcagca ttttgttcct gtgatgaaac ttgcaggaaa gggcaggatt gtatttatca
    10681 gctctcaagt ggtgcgagaa cctcgtcctt ttgcagcagc ttatacactt tccaaatccc
    10741 taaacgacat gtatgctcgt ttgctgggag aagaattgcg agaaagcggc gttggtgtaa
    10801 cacgcattgt tcccggatcg gtgaatactg cgacttgggg cgatgcagat gtgccgagaa
    10861 gtcagtttgt tcagcctgaa gaaattgctc aactcgtatc cgatatttgt cacatgccta
    10921 atacaacttg ggtagaagaa attagaattc gtccattgga caaaaactgg taatagctaa
    10981 gatggccaag agaaagctga gaaattttgc tgagttagaa acatttacca atgtgattca
    11041 gcatccgtat tacaaagaac aattcgatca tccactaaag ggcaaatggc atcaggaatt
    11101 cttccgcaat tcattgccca ttactttgga gttaggctgt ggcaaaggag aatacagtgt
    11161 tggattagcc attcgtcacc cggagcgaaa ttacttgggt gtggatataa aaggcaatcg
    11221 catttggcgt ggtgctaaaa cgggaattga agataacatt cccaatattg ctttcctgag
    11281 aaccgaaatt gagcacctga ataatttctt tttaccgggt gaagtagatg aaatctggat
    11341 tacgtttcca gatcctcaac ccaataaatc aaaagcacac aagcgactta cttcggccga
    11401 atttttagac cgttaccgca agattttacg tccgggcgga ttggtgcatt tgaaaaccga
    11461 ttcagcattt ttgtttgagt cgacgatggc agttattgaa tctcagaatt taccggtatt
    11521 gcgcattagt cgggatattg acaccgattt tccgggtgat gaattattac agattcggac
    11581 gtattatgaa ttgaaattcc gtcaaaccgg agtgcctatc aattacgtgg cttatacttt
    11641 atgaacgatc cacgcgacaa ggaagatttc tttgaaaagg tatttgccgt tgtgcgcgaa
    11701 attccagctg gtcgggtaac gagttatggt gcgattgcta gatttctagg gacaggagga
    11761 tcggccagaa cggtaggttg ggcaatgaat ttgtcgcatt cggctgccat tccagttccc
    11821 gcacatcggg tggttaatcg caacgggcag cttactggca agcatcattt tccgaatcca
    11881 gatgcgatgc aagaacaatt agaagctgaa ggaattgttg tgaaagacga taagattgta
    11941 ggtttttcta aagtattctg ggatcctcaa attgaacttg cgctttagcg tttgctaata
    12001 cgattgcctg tacttcgctg acgcttgcat ttgaagcgcg acacatccat gtctcttaat
    12061 gcaacatgtt tggtgtgacg acctgcaact cgagctctcc attttcgcca gttattggga
    12121 tgaagttcgc gtttcatcaa ggctttcacg tcgtcttcgc gtaatccaaa ttgaagaaag
    12181 atagcatcga aaggtgtgcg atcttcccag gccatttcga tgatgcgatc aatatcgata
    12241 ggttgaaacg aggctgtcat gcgaagagaa ccacaagaag cacgaaaagt tcatgactag
    12301 gatgtgattt tctttttagc caagacaagg tctacttttt cgcatgccgt tttgaagcgc
    12361 ttatcgtctt ttccggaaat gatgctgaaa gagcgcccat cttcctttaa agctatttga
    12421 taacgatcga ataattcttg tcgacgggtt ggatgttcac gcaaaggatc atttacccat
    12481 ggaagatcga tattcatgag taaagtatgc gtataacgtg gaatacgaga ggctttgagc
    12541 aaccaatcgg gacaggagcc aaagaccact tccatccaaa tgcggcacac gagtaaatcg
    12601 gtatcgcaga tgatgagtcg gtgatttttt tcgagtacgt tttcggcgtc catttgtccg
    12661 cgagcaatgg cttccacatc gtaagccgca tacttgcgat tgagttgcat caagtattcg
    12721 cgcgcatatt caggaactac cggacaacta taatgagccg ctaattggtg tgcaagggta
    12781 gattttccgg ttgattcagg gccggtaaca gcaattcgga tcatgaaagg acgaggtctt
    12841 ttttccagcg gaatgcgccg tacacagcca aagcagccat gatgatgtat aacaatgcag
    12901 ttaggtgcaa tgttttataa atgaacaatc cgctatttgc gagatcgacg agaacccagc
    12961 aataccagtt ttcgatgaat ttacgagcca tcatccaagt gatgattaat ccggcaacgc
    13021 ccatgctagc atcaaaaaaa ggcacatcgg tattggaata atgaacgagt gttaagccta
    13081 gcaaatagga ggcaaggaga aagagaatgg aaagtgcaaa cgcagtgttg agcggaatat
    13141 ttcgaacggg acgatcgctt gcttttttac ctaaccgatt ccaattccac cagccaaaga
    13201 aacctatcaa gacgtaggta atttgcaaac ctgtttcggc atagagaaga ctttgataga
    13261 aaaccggaac atagagcgcc gatccaaaaa taccgaaagc ccaaccggca atatgcccgc
    13321 gtgccgtgag gataacacct aagagcgcga gaagaaaagc actaagttcg aacagttgag
    13381 ctccgagcat gcatgggaat gagattcaca aacatcggtt aattcgtgtg attttaaggg
    13441 tgatttacac ggaagaatca gaataaaaaa atgttgaaat tggatgcatg aaatttaagt
    13501 ttctactcct ctttatttgg gtgtttcagt ttgcctctgc ccaacgtgag tcgtatagtt
    13561 ggatttatgg caattgtgat acaacaaaca attgtcaggg agtttatggt acagctatca
    13621 tcaagttcaa tgatgatagt attgaaagtg ttagaagagt tgaatttcca gtccccttcg
    13681 gatttttatc atcatcaata tcgaatgctc aaggcgattt aattcttgct actagtagaa
    13741 attatgtttt tgactcactt ggcaatacat tattagactt taggacaaat gactttaaca
    13801 atttgcaatt aggctattta aggcattcag ctttgttttt taaattgaaa gaaaacacaa
    13861 atgattatta ctttataaat tttaaagtag cgtggattga ttccgctgag aatccactaa
    13921 tgaataatcg tattagcaga atttactgta gcaaaattag ggttgatgaa aacggttacg
    13981 aagtcattaa agttgacaca ttgttcagaa gtgacacaat tttgggatct ggaattcaag
    14041 cctgtcgcca cgccaatggt cgagattggt ggatttttag caatacgttt aatcaaaaac
    14101 aatatttacg tggtctttta acgccctatg gattaagctt tgacatttat gatgggcctg
    14161 ggccaaatgt atttcagaac agtggtcaga atcattttag ctccgacggt acaaaacttt
    14221 tccattacat aactacatcc ttccgcaaaa tgcagatcta cgattttgac cgctgcacgg
    14281 gtgagttgag taattttcgg gagatggatt ttagttccta tattccttat ccaccctatg
    14341 attttacccc ttatgtatta agtccggatg gaagtaaaat atatatgggg aaatcgaatt
    14401 ctgattatac taattatgaa acaattcagg tagatatcga aacaggtcaa gtagctgttg
    14461 tcgcggatag tgtatttgtt cctttgctaa ctcctaattt aaaatgggtt atttctggtt
    14521 atcaagatgt attttctaca ggaattgatg atataagtgc tattaagaat cctaacaaca
    14581 ttggtactgc atgtaatcgt gttatattac ctaattgctt aacagttaat ggtgctatta
    14641 gcgaacccat cgaatacgcc aaccatatac ttggtccaat tgacggaagt agttgcgaca
    14701 ccttgggatt ggacgatgaa acgggaattc aaaaactaaa agattacagc tttattcttt
    14761 ttcctaatcc tgggggaaat caattaacta ttaaaaccga tttaccattg cctgtcaagc
    14821 ttattcttcg tgattcacag ggaaaaaatg tattcgaaaa aaaattcaat aaaaattcat
    14881 tttcaattca acaggaatta gtggaattaa atcaaggaat ttattttgtt gaattaatga
    14941 atgtggctca acataaacga ctggttagga agtggatgaa ggtggaatga actaattgat
    15001 attacccgtt gtgcggttga atcatcaatt taatatttta tttcaatcaa ttaaataaag
    15061 ctgttaaatc atcctgtcaa ttaatcattc atgtttgtct gctacgtgtt aattcttgtc
    15121 ttgaaacacg cttagacaca cttcgattaa ctcagcgtaa aagctcagag tgcattttat
    15181 gaagttcaaa tctggacgat gttgctcgaa cttttttcaa aacgattttg aaacaagcac
    15241 tcggattgta cgtacagatt aattatattt gcacgtaaac atttagaata tggacgccaa
    15301 attaacgctc aagcttgagc aatcggtgat tgagaaagca aaagactttg ctaagaaaca
    15361 gcaaactagt ttgtcgagat tgattgaaaa ctatctgttg agcattacca atgaagaaaa
    15421 tagtgaggag aagattaccc cattggttaa gagtttatcg ggcataattg atcttccgaa
    15481 tgatatagat tataaaaagg gatatgctga ttatctcagt aataaataca aataatggaa
    15541 cgtgtatttg ttgattctga tattattcta gatttattag gagggcgaga accattttat
    15601 aaatactctg ccgaactatt ttctttagcc gacaaaggaa aggttgaact acatgtttcc
    15661 tctttatcct ttgctaattt gaactacatt ttatctaagc aattttctgc tgaacaagcg
    15721 cgaaaaaaat tattgaaatt taaaacgctc gtaacagttt tgtctgtcac agataaaata
    15781 gtcgagttgt ctttagcttc tgattttaaa gactttgagg atggtttgca atactttacg
    15841 gctatagaaa atggaattaa actcttacta actcgaaatc taaaagatta taagactgct
    15901 caaattagtg taatgaccgc agagcaattt ttaaagggca attaaaaatc gatttagtaa
    15961 agagaaatac aactaatcca taattattaa cagcgtttct atagatgtaa aaagtctgtt
    16021 gaataaaaat ctacctcaca tcatttctac taataaacca agcccaagca attaaaacaa
    16081 actgtagcgg caagcgaatg agagcagcgg tatgtgagcc aatggcgggc gattcgcgaa
    16141 agacatccca aatatgaagg ggaagaaaaa gtatcattaa aactaaaatt cctaatgctg
    16201 ctttttgctt gtatttggca atgaataatc caattcctaa actgagttca attattcctc
    16261 cggcaatgtt tatcgccagt ttgggaagtg tgtcagggat aaatgcatcg taaaaagaag
    16321 gattaatgaa atgcataatt ccacccaaaa tcataaaaat tccgaaatac ttggttaacg
    16381 atggttttag gtttttcatg atttagaatt tgtactggaa tgatacatca atttaactta
    16441 ttctgaacga ctccaacgat ttcgaaattc cccttctgta tcatactgtt ctgcttgttt
    16501 ttgcatgttg aaaacacgtt gtcgtggatc gtttccaaca cctgctaaat atgcccaatt
    16561 gccccaatta ttggctggat cataatcgat taattgtgat tcgaagtatt cggctcctaa
    16621 tcgccaatcg agttttaatt gatgcaccag ataactcgca acattttgtc ggccacgatt
    16681 actcataaag cctgttaaag ctagttcttg catgttcgca tcaacccaat tattaccggt
    16741 atttccgttt atccaatttt ggaaatctgt ttcagattgc accatttctc gaggcggatg
    16801 ataaattccc aatccgtttt tgaagaatag tttagcgcca aattttagca gcatgtagcg
    16861 aaaatagtcg cgccagagta attcaaattg aagccagtaa gtcgatttat ttgctccaag
    16921 tttttcttct agtgatttta actgtgcgta aatagttcta ggagaaaccg agccatttgc
    16981 caaccaagga ctaaatttcg ttgaataatc agcaccgatt aatccatttc gcgtttcctt
    17041 gtagcgcaac atctgttgtg aaaggaagaa ataatcttcg attcgttgca gagcagcatg
    17101 ttctccaccg ctaaatggaa aggccgttcg agaatcggga attaggcggt tttctgaaac
    17161 cagtttttct atcaaaattc ctttttcaag atgcgaaaat ccgacaggtg aaccatactt
    17221 aaatggagcg cgaatgtgcc agtttgattc acattgctga cgaaactgag taaatacatt
    17281 gggaacttca tgtatcgaac aaaacaagtc ctcaggatgc atcaaaaaat cgtcgaaggc
    17341 tttataaatt gtaacgttca agtctttaag tgcatgcgaa acttctattt cctgcgccat
    17401 ttcttcactc gcacaatcag cagtaaaact tagctgttga atggcgtatt tgctgcagat
    17461 ttgtggcaga atttcctctg cttttccaga aaagattagc aattcagttc cttgttgttt
    17521 ccaagaattg gataaatcct gaagggtttg atatagaaac tgcttgcgga attttccttt
    17581 tcgcggtaat cctgaaaggg gaaacgtttc ttcttccggc aggaaatata ccggcaaaac
    17641 cgaccatcca ttatcggctg cttgagctag tgcaaaatga tcttgtattc gtaaatcggt
    17701 tcgataccag tgaagaagaa cgttcatgat tttgaatttc tacatcgctc gctgcaataa
    17761 ataacctgat cccaactgcg ttcccatttc ttccgccagg taagaggtcg attacaagac
    17821 ggacaaattt tttgaggcag gttcgctttt ttgacgcctt tcattcttcc acacccgaaa
    17881 cctgcaatcc agctttgtat gtggctaatg cacgttctct agccatttta tggtcaacta
    17941 ttggtcgcgg atagtgaaat ggatcgtcga attcgggaac ccaacgacga atgtattccg
    18001 cttgaggatc gaaacgttcg gtttgcaacg ctggattaaa aacacggaaa tatggagcag
    18061 catcgcaacc cgaaccagca gcccattgcc agccaccatt gttagcagct aaatcgaaat
    18121 ccatcagttt tcgagcaaac caagcttctc cccaacgcca atcgatgagc aaatgtttgg
    18181 tgagaaaact agctacaatc atccgcaccc gattatgcat aaatccagta gcatttaact
    18241 ccctcattcc tgcatccaca atcggataac cggttttacc ttcgcaccat tttaggaatt
    18301 cttcgggatt atttctccat tcaatgtgat tataagctgg cttgaatgca gaaactgcag
    18361 catgaggaaa atgccacaaa atcatgtggt agaaatcgcg ccagataagc tcgttgagcc
    18421 aaacatcact caattcttta gcttgtgaag ctaattggcg gatagatact gtaccaaatc
    18481 gtaaatgcac acttaagcga gaagttccta aacgtgctgg taaatcgcgc tgagtgccgt
    18541 atcgacgaat gatttcggtt tgaacagaac gagggaaagc tttgatagca gaaggtcgaa
    18601 agcccatttc ttccaaagtc ggaataggct tttgttggac tttgcagaaa tgagcgaaat
    18661 acttttcggt gggatacgat ttcaagtaaa aagccgagag tttttccttc catttcttgg
    18721 aatagggcgt aaacacggtg tatggcgttc cgttatcctt gagcacttcc cccttttcga
    18781 aaatcacttg atccttgaat gtatggaagt cgataccttg ggaattcagt aaaactcgaa
    18841 tggattcatc ccgatctttt gcatacggct cataatcgtg attggtgtaa acggcttgaa
    18901 catttccttc tttcgccact tgctgaaaaa cttcatccgg cgtgctgtaa tacacattca
    18961 gcgcagaacc tatagcttgc aactcctcac ttaatcggga aatttcttga tagataaaac
    19021 tcactcgtcc atcgtcatcg tcttccaaac gatcgaggat atttttatcg aaaatgaaaa
    19081 tgggttgaac ttccaatcca gatcggagtg catgataaag tccggcatta tcgctcaagc
    19141 gcaaatcgcg tctaaaccag aataatgctt tgtttttcat gaggttatta tctaggaatt
    19201 tcctgtggaa tgatacccgg tttagcgtag gtgagacggg aaactggatg tgttaaataa
    19261 tacgcatcta agccagcaga agtgattgtt ccagcttgat gaaggtcgat aaatccatct
    19321 tcacccaagc aatggtcagg cagttgaaga aattctattt tgcctgttac aagcacagtt
    19381 ccgttaatta gataggtttc ttctagtttt cgaacagcgt atttaacttg actttctttt
    19441 acaaacggaa ctagaatatt tggtaaaaat tcaggtgtta atcctacttc atcaaactcc
    19501 gaaacatcat ctggataacg cgcacttgtt tgatgggctg ctgtaataaa cgattcttga
    19561 atgtggttta tcgtgaacca gccaattgct cgaatgtttt gaagtgtatg tcgtccaact
    19621 gaatcggggc gaacaagcat accaaccaaa ggtggattgg caccaatgtg aaacaattga
    19681 gaaaatatgg caaggttagt tacaccatcg ggattaatgg ttccgatgag tgccacactt
    19741 ttaaatccat gcaccgaatt aatcagattt ctacgaaatc tttcgggaag tgtgaggata
    19801 tcgtcgagtt gatagttcat ggtttcaaat caaacgtaaa ctgcccatac ctccatcaac
    19861 tcctaaaatc tgccccgtaa tccagccttc gttttcgttc aataagaatt gtgctacacg
    19921 agcagagtct tcagcttgtc cgatacgacc gagcggatgt cgtttagctc cggcttcttt
    19981 cttttcgatt gtattgagga gtttttcagc tagaggagtt tcggttaatg aaggagcgat
    20041 gcaattaatt cggatgcgag aagaagcata ttcggcggct agggctcgag ttaaaccttc
    20101 tactgcacct ttcgaaacag cgacagaagt atgaaacgac attcctgttt gaacagccac
    20161 agtactaaaa agaacaaccg cagcacttcc tgatgctttc aaacctgaca aacattgctg
    20221 aatggctgat atagctccca tcacgttcaa ttgaaaatca ctttgaaatt gttccgtttt
    20281 gatgcgatgg aaaggttgta gcgtaataga tccgggacaa taagccagtc catgtatcac
    20341 ttctggcaat ccagctagag agaattcctg actcacatct gcttcgttaa agtggatttg
    20401 agaagaaacc tgcacatttc ggatgttacg tgcatacacg aaaacctgat gtccttcttg
    20461 agccaatagc tttaccaaag cttcgccaat gcccgatgta ccaccgagta ttacataatt
    20521 tttgggttca ctcatttcag gttgacgtaa gtgaaaaatt ccttgcgtgt ttcgtcttga
    20581 ttaaattttc caccaaaatg ggaagtgaca gttttagagc cattatcctg aactccacgt
    20641 gaggatacac acaaatgcac agcatcaatt acgactgcaa catcatcggt tttcaataca
    20701 cgttttaatt cgttggcaat ttgtaccgtt agtcgttcct gcacttgcgg acgatgggca
    20761 aaatattgga caatgcggtt aattttcgac aagccaataa catgaccgtt ggaaatataa
    20821 gctacatgcg cctttccaat gattgggaca aagtgatgtt cacaattgga gtagaatgta
    20881 atatccttct ccacgagcat ttctttgtat ttgtatttgt tttcgaagag cgcgatttcg
    20941 ggtttattct tgggatcgag tcctgcaaaa atctccttca catacatctt agccacacgt
    21001 ttgggagttc ctctaaggct gtcatctgtg aggtctaagc ccaaaatatt catgatttcc
    21061 cggaaatgct cttctatccg gatcattttg gttttatcgt ctatgtcaaa agcatccgca
    21121 cgcaatggtg tttcggctgc tgttgaaata tgttcatctc caatgaggtc ctcaagagag
    21181 atatcaggct ccgttgtatt caacaaagtt tcgttctgtt tcataaagtc tgacagttaa
    21241 agcatatcgg tcgttcaatc tctcacgtat tcgattccag attacgactg ctatgttttc
    21301 ggcggttggg tttagggttg caaattcggg tatgtccaag ttgaagttcc gatggtcgta
    21361 acgttcgatt acttcctcgt ggataatatc gcttagaatc ttcatgtcga tcacataacc
    21421 ggtatcggaa tcaatttctc cggtaacatg cacatcaagt tcataattat gaccgtggta
    21481 attggaattg ttacacttac cgaaaatccg tttattggtt tcgtcatccc aattgggatt
    21541 atgaagtcgg tgtgcagcat tgaaatgttc tttgcgaatt acggtaacgt tcatagttag
    21601 ggaaggaaac ggaggtttgt atattttgtt cattcgggaa gatatttaca tctaatccga
    21661 actgttttga aagatacagg aaatctcaac cttttcgaaa catgcttgtt cgaggcacat
    21721 gtcaatcctt aatcctatct atttatttaa gcgagctagc caagcaaatc ttccagatag
    21781 tgccgatgtg gtgattttgg gttctggatt aggtagctta agtgcagccg tggttttggc
    21841 taagcaggga aaacgggttg ttgttttaga gcagaattat ttacctggcg gatgtgttag
    21901 ttcgtatttc cgacacggag tgattttcga aacaggagca acgactttag ttggtttgga
    21961 tgaaggaatg ccactttggg aagtgattcg gcaaacggga atttcgattg aagcgcaacg
    22021 attggattta cccatgcaag tggtattgaa ggatgggacg attgttcggc ggtttaatga
    22081 tccagaatta tggattgccg aggcagaacg agttttcgga gtgcaaggac aacgtgcatt
    22141 ttgggaaacg tgcattcgtt tgagtacgca ggtttgggat atttcaaccc gtcaactcag
    22201 ctttcctcct gcatctttaa ccgatatttt aggttgcatt aaaagtgcgc gaatgaagga
    22261 tttaagcgca ttgccttatg cttttaaaac cattcaggat ttgttatttg aaaacggctt
    22321 ggattcgaat gaaacatttg ttgcttttgt gaatgagcaa ttgatgatta cagctcagaa
    22381 tacatcgcag gaagtgaatg ccttatttgg tgcagcatcg ctctgttaca cactcgtggg
    22441 aaattattat gtgcctggcg gaatgattcg tttgattgca ccttttgtgg agtttatcga
    22501 gcaaaatcgg ggtgatgtgt tattgaaaac tcctgttagc tcgatcaaac gagttgctaa
    22561 tcagtgggaa atccacacgg gtaaaggaat tattcggagt cgagcagtta tttcgggttt
    22621 acctgtgaat aatcttgtgg aattggtgca ggatgagaaa tcgattcgaa aactgcaacg
    22681 gaaattaatg cctattaaaa gcctgaatgg agcgatacaa gtgagtttcg tttaccgcca
    22741 acgcaaggaa gtgtcgtgtt tacaccaaca aattcatctt agtgaatcgt tcgaatttga
    22801 gggttctggg agtattttta ttagtaaaag tgatgtaggt gatcgcttgc gatctgaact
    22861 gggagagggt attttgtcgg taagcatgca ttatcgatta gggcagaaaa gcaaccttga
    22921 tatggaacga gtgaaattgg taattcagtc aattttgatt cgggaaggtt attttgaaga
    22981 agatgatatt attcggataa gcgcgtcaac accagcttct tgggaagatt ggacgatgcg
    23041 aaaaggtggg tttgtaggag gatatccaca gcggaaaaac attaagcctt ggcagatgat
    23101 tcaagctcgc gttggaagtg gtttgtatgt atgtggcgac agtgtatatc ccggacaggg
    23161 aattcccggt gttgcattga gtgggctgat agcggcaaaa aagctggttc aagacgggta
    23221 ttgagatatc ttattcatac aaaataatgg ggaagtgttt tacctgttaa agcgaaagga
    23281 tgaatactat atttgttatt ctgcgcttcg caggtattca cctcttttta gattgcatat
    23341 gaaaaaaccc cttttacttt ttgctttgag cgctattcct gtgcttagct ggggacaaac
    23401 ccaactgagt ccacaggttt tttcgtctca gggaggagat ttcagcaatg cccagtttgg
    23461 tgtgtcttat acgattggag agatgtcgtc agtttcgact atttctaatg gtaatttcac
    23521 cctaacacag ggatttcacc agccggataa atatcagatt gctttagtgg aatcgataga
    23581 tgctagttgg aatgccgaag tttatcctaa cccagcagac gagcagttaa cgttgaaact
    23641 ctcttcggag aagatgcttg atttgacact tgatttatat gatgctgaag gccgtaaagt
    23701 gatggcaagt cgcaagttga atcaggttcc tggtacacag acttacaccg tagaaacatc
    23761 cgatttagcc gctggagctt atttgcttcg catgacaagt gttgatggaa aacaccagcg
    23821 cagtttccgg attacaaaaa tgaattcctg accaattttt accataaacc atgaaaagaa
    23881 atttccgttc acttctctta actcctgctt tagggcttat gctcttagct ggaactgtgc
    23941 aggcacaaac gcctgtgcct aatggaatta attaccaagc tattgctcga aattcgtcag
    24001 gtagcgtttt tgtaaatcaa accattagcg ttcgaatttc agttttacaa ggatctgcaa
    24061 ctggtcctgt tcaatattcc gaaaaacaca cagctactac caattcattt gggttatttt
    24121 cctttaaaat tggtggtggt actgctctaa ctggaacatt tgaagacatt acttggggta
    24181 cagctaacca atatgtaaaa gttgaggttg accctacagg tggcacaact tatacaacag
    24241 ttggaacgaa cgaattacta tcagttcctt acgctcttta ttctgaatca tctggtaacg
    24301 gtggaactcc tggccctcaa ggtcctgctg gtcctgccgg agcaactggc cctgctggtc
    24361 ctgctggccc cgccggccct gctggtgctg atggacaaga cggccaagat ggtgctaccg
    24421 gtcctgctgg agcaactggt cctgccggtc ctgctggtcc acaaggtatt caaggtcctg
    24481 ctggtcctgg aagtggttta aattgctggg atacggatgg tgatggtgtt caagatcctt
    24541 ctgaagatgt aaacagcgat ggattctgga atgctctaga ttgccgtgga gctcaaggtc
    24601 ctgctggtcc aactggagtt gctggtcctg ccggtgctga tggagccgat ggtgctgacg
    24661 gtgcaactgg tcctgctggt gcaactggcc ctcaaggtcc acaaggtatt gctggagttg
    24721 ctggtcctca aggtcctgct ggccctgctg gagcaactgg tcctgctggc gcacaaggag
    24781 caactggcgc tacaggtgca actggattaa caggtcctgc tggtgcaact ggagctactg
    24841 gattaactgg ccctgctggt ccaacaggtc ctcaaggtcc aatcggattg acaggtgcaa
    24901 caggcgcaac tggtcctgcc ggtcctgctg gcccaactgg tttaacaggc gcaactggtc
    24961 ctgccggtcc tgctggccct gctggtgcaa caggagcaac tggtcctgct ggtccacaag
    25021 gtcctgctgg agctggagga acattggatc aatcgtatga ttttggtgga gctggagctg
    25081 gaaaaacaat tactgctgat tctggtcctg ttactattaa tgcttctggt actggtttaa
    25141 caggtattgg tttacaaata aacgagagtg gaacagcaac ctcaggatta gctgttgtgc
    25201 tatcgggaac tgggactgca attcaagggg catctacaaa tggggcaaat acattcgcag
    25261 caattcaagg gacaacaaac agtacatccg ttgccaattc ggcaattctt ggtttgtcga
    25321 cttctgcagc atctggtgtt tcaggtcaaa taaatgcagc aggaactgca tatgcaggtg
    25381 tttatggaaa taacttaaga actggtgcag gtgtaggtgt tggaggaatt ggtgaaactg
    25441 gtattggtgg agaaagttct tcagctacag gaactggtgt atttggtata ggaaacaata
    25501 ttgcttttac ttcaggaaca gtcttggcag acggacaagg tggagcattt aatgggttca
    25561 attttggact ttatgctaga aacctaaact tcgctccagg tgttgatcaa gctgcgattt
    25621 atacaaatga tggagaccca tctaatactg gaacgggtgt aattaattgt tggactgctg
    25681 cagatgtaca ctacaaaatt cttggtggtg gtactgtctc aactatagta aaagatgtta
    25741 atgacaatgc cgtagtaatg catgctccag aagctccaga agtattattt gaagattttg
    25801 gaagcggaca acttgtaaat gggaaagcac atattacgat tgatcctaca tttgcgatga
    25861 acattactgt gaatgaaaaa catcctttac gtgtttatat tcaagttgaa ggtgattgta
    25921 aaggtgttta cattacaaat aaaagtgtga atggttttga tgtagttgaa ttggcaggag
    25981 gaaattcttc tactgttttt cagtggcaca ttgttgcaaa ccgttctgat aaaaaagaag
    26041 gaagccgtgt tgctcgttac gaagaccttc gtttcgaatc aactcctaaa cttcaaacat
    26101 tgaaaaatgt tgatcctctt cgtaaaaaat aggaattgat atttattctt taaaaagggc
    26161 gtttgtttaa tagcagacgc cctttttgtt tttaatagaa gattgaatag cggtataaaa
    26221 tcgaagattc attattcgtt tttatccttc gcatttgaac ttagaagaag tggtaaggat
    26281 tgagcaaaga gcccgcacga aggaggactc gtcgcgaaag cctggtcacc accctaatct
    26341 ttattcttgg agcttaatgc gatgattgca gctgcgtgtc ggtccttttc agcattggat
    26401 ttttgaccta gtttttccca tacttcggca cgtccgagaa gtgcttcggg atcgagtgca
    26461 tttaagttga ccgaagtgtt ataatcgttg agcgcatcct gcaaacgacc cgaaacgctg
    26521 tgttggtgag cacgtgcgga ataataatat gagtacttag gttcaagacg aatggcttga
    26581 ttgatatcgg gcattccatg ttgctcgtcg aattgattga aatacacgac agcacgttga
    26641 taccaagcat cggcaaaatc gggttttagg cgaatacact gcccaaagtc gttgatagca
    26701 gccggaaaat cgaggaaagg ataggttgct ggacgcgttt caccttgcgg agtgaaaaat
    26761 cgggagaaga gtaaacctcg gtaataccaa gcgagatgat tgctcgaatc ggcttgaatg
    26821 gcttgattca ttgcttggat agctagtgga aaacggtcgg agaagtaatc catgcgtccc
    26881 atttcgatcc aggcgtcgga acggattcga cttgtaggag aaataatgag ttggcggaga
    26941 gacttgcctg catttgtggt gtcggtgtag atgatttgca agcgggcaag atcgagacgg
    27001 gcggcgtcac agttgggatt taggagaagc gcttgtttaa aatcgcccat cgcctgagag
    27061 tcgttttgga tcttttggta gcagcgtgca cgaagtagaa aaatgctatc gctttgctga
    27121 tctagactga tacaagtatt gagttcgatg attgcaccga ggtaatcttt ttgctcccat
    27181 tcggtttgag ccgatttcca atgatctaca tccgattgac aggctgcaaa gcaacaaaca
    27241 gccataagaa ttccggaaat agcagtaaat cggaaaaaag gaatcatgct gattatagca
    27301 acttaatttt cttcgacttg ctcttcatta ctggtattgt cgagaagcgg ctgaggagca
    27361 ccaccattga gagaaatagt agctaaggct ctaacttgtt caaaatctcg ggcagacgta
    27421 tcaatgccat cgtaacgcag gtgatgaata aactgccgaa tcatatcggg actttcggcc
    27481 atacgacgtt gctgatgtgg agcgaggtag ttgttaatgt tgacttcttc ttcttgtccg
    27541 ttttcggtat tcgtacgcca aatttgaact tgagcttctt tttcgtgcag gcgaatatcc
    27601 cagctaaaat ggaaagcagc atcagtccaa cgaccatttc cccaataaag gaaaagcgtt
    27661 accggaatga ggatttgcgc acttaagaaa atgagtaaaa tgcttggaaa gagtcgtttt
    27721 ttgcggtatt gcaacatgta tgaaccgccc ggctggaaca cttctccttt gaaactaaat
    27781 aggtcgtaaa gcaggtaaga aatgcctgaa atgaggcggt ggattttttc ggcgggaaga
    27841 aaaaccgtgc aggaaaggat agtgaggata gggaagaagc ctgcaggaaa aaggattaaa
    27901 gctgacagtt gaataagaat taccattcgg aaagctccgg tactggtgga tttatccaat
    27961 agaaaatgcg gaataatgaa atcgaaaaga atcagaaacc acgaaatgac aacaggaaac
    28021 caaccagcca acatccaagc aggaaaatca acacccatgt tcagggataa gttttcgagc
    28081 cagatattca tagggcgacc ttcgaagagc cagtcggcat tcagtttcgc cattccagca
    28141 aaaatgaaaa cgagtgctac ttgtaactga aaagcaagaa tgctccaacg aggaataaaa
    28201 tcgacacgaa gcgatggttt tctaagcaag tcgatggaaa atagtcggtg agcgggagaa
    28261 atgctcaaca tgatcgacaa aagcagaacg taataatagt agctcaaata atttgcctga
    28321 tcgataagcg aaacgtaagc aaatgcaatc gtaaagatga gcgttgaggc gcgaaagaac
    28381 caaccggcac caatcattac tgccgaagcg aatagtagga taaacacgcc atacatgccg
    28441 ggagccgaaa gcggtttaat ccaagaaagg ccgggataac tgaaatagta attgggttgg
    28501 atgaaaaagt cgtaaacgtt tccgctgaac aacatataca aagcccaaag tgccatagct
    28561 acaccaaagg caactcgaaa agcaattaag ggatctaacg caactggctg aaacagaaag
    28621 gttgtgcgtt gagcaaacca accagctttt tcatcttttt ctggttccat catgttcatc
    28681 ctctttagga gaggtggaag atacgcgatt ttagcaaata ccgaagccat aaaggataat
    28741 ctccttgcat gaatttcgtt tctttgctaa aaaattatga agcgctattt cttccttctg
    28801 ctattagtat tgcctttttc ggcttctatt gctcaatcgg ccaagatgcg cctaggcttt
    28861 actgctggac caacactagc ttggacttct tccaacacga atattactac taattttttt
    28921 actgtaggtc gagcaggtgt gcagttgggc gtaactaatt cgcttgaatt aggagatcat
    28981 ttttttagtt ctgtttcagc attgtattca cttcaatcgt ttggattaaa acaaacagga
    29041 attccaagtt ttgggattga tgcccgattt aaatcgaata accttgagtt tcctttaatg
    29101 ttcggaactt gtgggtattt gggctcgctt cgtcaccgtg agtttgtggg agcaggcttg
    29161 caaatcaatc tgtcgaatgt gcaaaccgtt aaactaagtg gcgatagttc tactgtacta
    29221 acttatacca cacaaacgaa ttctctgagc aatataaatc cggtatttat tgcaggtttc
    29281 gaagtaggtt ctatttttaa aagtgatgcc gccttgtatt tcggagcaac tttccgctat
    29341 gcaactaccg atgtgtatca aggtcaattt accagcaacc gttttcctgt tcagctccca
    29401 acttataacg gtacctactt gagcttcaac attacgtatt acctgccgcg ttattcgtac
    29461 tggtttaaac gggagtttat ttactaatca acgggaaatc cactccgatg gattaagctt
    29521 cactttccct ttccagattt cgaggtgagc ctccgatttt cctcctgaat catccttatc
    29581 ggcaacaccg attgcttgtc ctgtatgcac tttttgtccc ttcgaaacgg agctagaagc
    29641 cagattacta tatacagtta ggtactctcc gtgtttgaga attaccgcca tattagcacc
    29701 tggaatgcta attacaccag taacttcccc atcgtaaata gcttttacgt tagatccttg
    29761 tggcgtcgca atgttgattc cgttgttgtt ggtggtgatg ccttttaaaa ccggatgtgg
    29821 atgcgtacca aacgattcgg taattacacc acgatctact ggccaaggta gtcgtccttt
    29881 gttggcttcg aattttccag ataaggcacg agtttcagat gtaacacgca tttcgggttc
    29941 tttcaccggc gctggtactg gtttgcctga cgcttcggct tttttacgag cagcttcagc
    30001 ttccgactta gatttagcca ttgcccgatc tcgttcccga tttatctcac gttcaattat
    30061 tcgttgaatt tctccatcga gtttcttagc atccgctcgt ttcttatcga ggtctttctt
    30121 gagcgatttt tcctttttct gaagagccgt aagtgtttgt tcctgctctt tcttttcctc
    30181 acttaatacc ttcttttcgc gctcttcact acccaataaa tcgcgtttag tagataaatc
    30241 ggtctttagc tgttttaccc gaccatttag ctcatcctgt gaagcttgaa taagcaaagc
    30301 ctgttcgcga cgataattgt tgtatttgcg caaataatac aggcgtttat atgcttgctg
    30361 gaagtctttt gctgagaata ataataccag cgatgattgc tgatttcggg ttagataagc
    30421 ctgtcggatg agtttaccgt aggataattt gagtcggtcg agctcgattt gtagctcttg
    30481 gattttttgt ccgttcgtgg tgatagtcga gttgagcatg cccacttcct gttgaatggc
    30541 attgatcaaa cgtttacgat cgtcgagttt tttattgagg attttaagtt gaccgaggct
    30601 gagattttta ttcttcttgg tttccttgat gagtttattg gtaagatcaa tctctttttg
    30661 gaggctcttt ttttttcgtt ccaattcgga gcgactggtt tgagcttgaa cttgggatcc
    30721 aagaaccaac agaaggataa tacagaagaa tctagcgact aggttcatag ttttcaggaa
    30781 tgttgaatgg taagttcaac tccttgttaa cggtaattcg ggaatattcc agctcaatct
    30841 tcacttgttt atcggcctga gcatgtaccg ataaagcatt gggtacgcga aatccttcca
    30901 gatcttcaaa gcctttgtag gaaactgtaa acttccgaac cgggcggtag tcctgcattt
    30961 ccatctgtac gattttgttt tttaacgccg aaaaccaaat ctcctgcgtt aaaatctgag
    31021 ggatatcgag ctcgctctcg cgacgtatcc tacgcttctt caaggtgctg agaacaagca
    31081 aatcattttc tcggtcgctg atataatttt caacaccgta atgtagatag gcgttaccca
    31141 acaaaattga ctgtaccatt ttgaagttta cttcgatctg taacagactg ttgaggtatt
    31201 gataattccc tttgaaatac gtgccgttaa tacgattgat gaattttaac gaatcgggag
    31261 tgattaaagc tcgtgctact tcaatcccta atgcgggtga aatcgacatc caaatggcac
    31321 tgtctttttt caaacgcaaa ttggctgaga aggaattcgt ttgtttgtcg atatcggtat
    31381 tggcagaaac cttagcgcta aaccatttta gtggaacgtc ggcagcatat aatgcacgag
    31441 aaagactgtc gggatttgaa ctaaaagtac ctgtcggctt gggtgttgtt tgctccggta
    31501 gaggtgttac ttgagcaact ttttttgatt tacaagcaac gctgttaatc gtgaatacac
    31561 atactaaaag ccagattgcc ggcagaattg ctttcttcac ttactccgaa tattttttct
    31621 gacttatttt ctgttctagt ttatcgcttg ttccaccgag ttcttttgca cgattccaaa
    31681 aatctagtgc atccgtagtt ttcccttgtt tgattagaat gtctcctttg tgctccatga
    31741 tggtgccact tcgaattcca ccattttcga tggctttgtc aatccaaata gaagcattct
    31801 ctaaatcatt catttgaaac aatatccaag cgtatgtgtc ttgaaaagaa gcatttccgg
    31861 gttgaagttc attgcatttt tctgccaatt ctttcgcacg aggaaggttt tctttgcgta
    31921 atgacaagta atacgcgtaa ttgtttaata cgtatgtatt tgctgcatca tattttaatg
    31981 ctttctcata cgcatcgttg ctttcgcgga atttagtcat ggaattataa ctatctccca
    32041 ttgaagcata aaactgcgac gaaagtgccg aattaccaat cgttacatcg gctcctccct
    32101 taagtgattc tatcgcttta tcgtactgtt tcaattgcat tgcggcaatt ccgttataaa
    32161 gaaatagcga aggctgatag ggaaacaact caatcgcttc tttactcaaa tcgaacatgc
    32221 tttgataatt cttcaattcc gaatcaatca gcaaaatttg attccacacg ttataccgac
    32281 ttttatcagc cgctaaaacc aacatgtatt gttcgcgtgc ttcgtttagt ttgtcttcgc
    32341 gaagcagaaa atcaccataa acagcatgtc cttgaggttc ttcgggatga gccgcaacta
    32401 attcgcgaca taattccatt gcctgatatt tcaactccgg atattgtgct gtgatttcga
    32461 aataggagga aagcacctgc atcttcaact ccaaagaact ctccgggttt gcaaaagctt
    32521 tcttcaattc ttgatacgat ttctctttgt cgccttgtga acggtagtat tcggctaatg
    32581 aaaaacgaac gttgggattt tccgggtcga gacgtaaaat ttcgttgtat tcggctagag
    32641 ctttttcggg ctgattagtt gcttcgtata tttctgctaa aaatccacga tacccaactt
    32701 cttcggggaa agcagccatc aagcggttaa tctcagcaat ggctttatcg gttttaccca
    32761 aacgcatgta gaggtttttc ttttgaagga ttaactcttc gttaattcct actaatgcct
    32821 ctaatttgtt gtagactttt gcagcctcat caaatttacc cgtttggaca agcgctgcgc
    32881 ctaatccgta caaatagtct tcgttgttag gctctttttt atgcagactt tccatgagcg
    32941 gaacaacttc ggcgtatttg tcattgcggg cataaatatc cgccagggca agcttgtacc
    33001 atttgttttt ctcatcaaac cgcaccgcat ttttcccact tatttcagct aagtctttgt
    33061 tacctgtttg caagtaaact tgacttaatt caaaatgagc cgcagcagca cgagggtcaa
    33121 tccggatggc ctgttggaat tttgtttgtg cctgactgaa gtttccgagc agcttctctt
    33181 tttcggcatc aaaaaacaac acttcaaaat cgaggcgttc cttttcggtt aatgccgaag
    33241 cggtacttgg attcttatcg gccgaagcaa cctgtttgtt tttacaggag gataaggatc
    33301 caattataat gcaggaaaaa agtatcaggt taatcgggcg catgcttcag ttttagttcc
    33361 cggttgagaa aacggtatag tcaccaacac tcaaatcgag tttttggttc atgatttcaa
    33421 ccgaatttcc aatcatcgag ttagctataa cgctgttttg gatgcgagta tttttctgga
    33481 tgatggaatt ctggattacc gaattttcaa tcaggcttcc atcagcaatc gaaacatgtg
    33541 gaccaaccac cgatccgata atcttaacac cttttccaat gtagcaaggc tcaattacta
    33601 atgcattttc ttttaccaaa gatgcatcaa tcaattgttc gccttttttc aattccaacc
    33661 agcgagaatg tgtgtaaaca gtagcgtttt tatttccaca gtcgagccat tcgattactt
    33721 tgccaggggc gaatttgagc cctttttgct tcatgttttc gagggcgttg gtgatttgat
    33781 attcgccttt ttctttcagg tcgttatcaa tcaaatactg caattcggca cgtaaattct
    33841 cgccatcgcg gaaatagtaa ataccaataa tcgcaaggtc ggaaacgtat tctgcgggtt
    33901 tttctacgaa atcggtaatg ttgccttctt cgttcacttt caccacacca aatgggcgag
    33961 gatcttctac ttgctgaacc caaatcactg catctttcga ttcgtcgaga acgaaattcg
    34021 ctttgaaaag tgtgtcggca aaagcgatga tgagtttacc ttgcatcgat tcggctgcac
    34081 acaaaacggc atgagctgtt ccgagcgctt cttcttgata ataaatgctg ccttttgcgc
    34141 ctactttttc ggcaactgca atcaggcttt tttcggcttc tttaccaaag tttccaatta
    34201 caaacgcaat ctcttcgata ggttcgtggc aggttttggc gagttcttcc acaatgtggt
    34261 gaaccatcgg tttgccagca aattcgatta aaggttttgg ggtgacaagt gtgtgtgggc
    34321 gcatgcgttt gcccatgcct gccattggta taatgattct cattcctgaa aattagggta
    34381 ggttttttta atgaactccg gtatgaccaa atcctccagc accgcgctct gaatcggcaa
    34441 ggctagaact ttcttcccat tcggcacgtt cgtgtttggc tacaaccaac tgggcgattc
    34501 gctctccatt gttgattgta aaagcagtat ccgataagtt taccaggatt acttttattt
    34561 ctccgcggta atctgcatca atggttccgg gtgaattgag caccgttact ccatgcttag
    34621 cagctaatcc gctacgtggg cggacttgtc cttccattcc gacagggatt tctaaaaata
    34681 aaccagtagg aattaatgcg cgtccaagag gtgcgagaac aataggttcg gttaagttgg
    34741 ctcttaaatc acatccggcg gcatgttcag ttccatattc cggcagcgcg tgtgccgact
    34801 ggttgatgat tctgattttc ataacgggat gttttcttag gttgaagggg acgaaattac
    34861 ggtttttttc cttacttcaa gaagccacac agcgccaaca aatgctaaca aaatcaagca
    34921 tttcagcacc catgagatag cgccttccat gggaagatac ttggtgtaaa gccaaaccat
    34981 aagagcaaca gagacaggat acccaattac tcgacgtaaa tcgtattcga ttgggtaaaa
    35041 tttttgtccc aagagatacg aggcaattaa catcgtcaaa tacacaacaa acgtagccca
    35101 agaagcgcct acatagccaa attcaggcac ccagagatag ttcaaaatca aggtaagtac
    35161 agaaccgaaa attgctagcc aagcgccaat atacgtgcgg tcgttaagct taaaccaaat
    35221 acttaagttg aaaaacacac ctaaaaacag ttgcgccatg agcagaatgg gtacaaccac
    35281 caatccttcg tggtaggctt ctccgatgaa ttctttgaca atattcatga agatgagtac
    35341 acccaaataa atcaaactac aagcgattac aaaccaatgc atcacttcgg cgtaaagctt
    35401 tttagcgttt ttctctcgtg cttgtgtgaa gaaaaaaggt tcagctgcaa agcggaaagt
    35461 ttggataaaa agggtcatca gaattgccac tttgtagcag gctccaaaaa ctcctaaatc
    35521 gtgcatagca atggatttag gaagctggta tttcatgctc atccgactaa acatttcgtt
    35581 ggttattccc gccaatccac caatcaataa gggaaatcca tagcgcatca tacgtcgcca
    35641 aagttcgctg tcgaatttcc agctcatttt ccacatatcg ggaataagta gcagaaacgt
    35701 gaccaaactc gccaccagat tggcaatgaa cacatatcct acacccatgt tcgggtcgta
    35761 gatatcacgc acagcatcct gaatagatgt actgccgttt tcgagcaacc acggacaaag
    35821 catcaagaaa aacacgttga taagcacatt cacaccaatg tttaccaatt tgagaatggc
    35881 gaatttgagt gggcggtttt cgagtcggag tttggcgaat ggaatgctgg tggcggcatc
    35941 ggtggctaag atgagtgcaa accaaacgat gtattcggga tattcgggat attggatgta
    36001 atcagctatt ggtccgacaa aaacccaaca aaggatgaaa aatccaagtg agctaactaa
    36061 aagtgaaatg agcgatgtcg cataaatacg cggacgatct tcttgattac tggcgaaacg
    36121 aaagaaggac gtttcgaaac catacgtaag cacaatcatt aggaaagcaa cgtaggagta
    36181 catttcagta actacaccat atgcttcggg aaggaaaaca cgcgtgtgta gcggaaccag
    36241 caggtaattc agcagtcgac caacaatgga actaagtcca taaatagccg tttgaccagc
    36301 aagtttacgc agtggattca cgctttatca atcatttgct ttagcaacaa aggacgtttt
    36361 tttcctatca atctccatca catttcgaac atccgaatat ttcggattta agagtgagtt
    36421 atttcccttt gaattcagct ttacgctttc ctaaaaaggc ttcggttcct tctttaaaat
    36481 cttcggttcc aaaacatcct gcaaactcac gaatttcggt gtcgaaacca ttttcatcgt
    36541 actgataatg agcattcacg caacgaatta ccgcagcaat tgctaatggt gcttttgtgc
    36601 caatcttttt aagtatttcg gtggctttgt taatcaattc ggcttgcgaa actacatggt
    36661 tgactaaacc taaacgatgc gcttcttccg cgccaatcat atcggcggtc atcaacaatt
    36721 cgagcgcttt tccttttcca ataagctgca ccaaacgttg tgttccggca tatccgggaa
    36781 tcaaacctag atttacttcg ggttgtccaa agcgagcatt ttcagctgcc acacgaattt
    36841 gacacgacat cgctaattca catcctccac ctaatgcaaa tccgtttacc gcagcaatga
    36901 caggcttagc cgagcgttcg atgccagtaa aaacatctcc accgtcgcga cttaatttct
    36961 ccgcttcgat aagtgaatac gaagcaaact cactgatatc ggcacctgca acaaaggctt
    37021 tttcacccga accggtaagt atcattccga acacatcggg gtttttattt ccttgtgcaa
    37081 ccgcttgacc aatctcctga atagtttggt aattcagggc attgagttta tcgggacgat
    37141 taacggtgat ggtttgaatg ccttcgttta cagaaacgag aatattttgg aagctcatga
    37201 tttggattta agcgtgtatt gttgaagttg tggaccactt gggcgaagtc ggcctttgcg
    37261 gtacaacata ttgctgtatt ggtagttttt atttttgcgg agttctttct gagtttcttc
    37321 aggtagggca ataatttcca tgcactcagc actgcagcag ccgttgaatt ctttttggca
    37381 attctcacat tgaataaaaa gcaggttgca atctacgttc gcacaattcg tgtgcgtatc
    37441 ac
//
LOCUS       DAOOQW010000005        36041 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_1363_length_36041_cov_5.716668, whole genome shotgun sequence.
ACCESSION   DAOOQW010000005 DAOOQW010000000
VERSION     DAOOQW010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 36041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36041
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1363_length_36041_cov_5.716668"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            42..5456
                     /locus_tag="PK281_00830"
     CDS             42..5456
                     /locus_tag="PK281_00830"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HOY47909.1"
                     /translation="MDTTDLDSDGDKDLISGGLYCLSWEENRGNGLFVNHVISQLQPE
                     VQAVVGIDLDQDGYQDIISASLSNNAIYWNRNNGMQTFSSTTLNSTAAGPAGLAAADF
                     DNDGDIDLAVASYTGNKIFWLRNNGSQSFTQVDLLSNFNGPTKVRTGDMDGDGDQDIL
                     ASARTGNQIVWLRNNGSGVFSTVNISNSLTAPREVKLRDYDQDGDLDIIYASDLGWGW
                     FRNDATIFNIFPYSTGTGIYSVELADLNNDGHLDFVTANYSSELINYSLNSGNQTFSS
                     GGTIDNRTSYPNQLVCADYNSDGKVDVMCGGGLDIRYCQNSATLSFTAKQVMQYAGNN
                     HGACHGDFDNDGDVDLMEVGFTFMYWYRNESNGEFTPIQLQDNGFSYITTNYGVNIRT
                     ADMDGDGDEDAIYTENDNNKISWIENKGNGLFAYHFAASVMGPYSLEPIDFDDDGDMD
                     FVSGSTADDAVYWYENNGSQTFTEHMVSGMYWDPFSVRVFDYDQDGDMDVFSAQGNPT
                     NKLIVFDNDGNNLDFTDYIIDNSAPGINSIDVKDVNGDGDLDLLSASSTDDKISWYES
                     SGGSFPSFTKKTVSTGVDGATYVFGGDYNGDGEMDVISTSTVDKKVSVYINDGSQSFN
                     RINIGNQVDGADFVESGDLDGDGLEEIYATGSTHGIVQIFKSTPFVLPPAVDLTPCGD
                     IFISEYVEGSSYNKAIEIYNPTPNPIDLAPYRILFYANGATTATQTIVPSGILQPNDV
                     WIIAHSLSDLEFFLAADIDATLNFNGDDAIALTKNGDIIDLIGVIGQDPGTAWTGTSG
                     ASTLDKTLVRKPEITKGNNIQGSSFDASVEWIVYPIDAYQYIGNHTNVCNGACIPSIS
                     ISASSTDICAGTSVTFTASVTNEGAAPTYQWKKNGSVVGTNASTFSTSSLADNDIITC
                     ELTSNAACAIPGAISSNSIVMNVNVSSASSISISASATSICAGTSVTFTATPINGGTT
                     PIYQWKKNGGNVGTNSSTFNVSTLVNGDIITCVLTSNAACVSNTSSTSNAITMIVQSS
                     VTPSISITASTTTICAGTSVTFTTNAVNGGTTPSFQWKKNGGNVGTNSTTYSSSTLVN
                     GDIITCELTSNATCVSSAVVSSNSISIIVNPIVTPSISISASQTTICASTSVTFTATP
                     VNAGSSPSYQWKKNGGNVGTNSATYTASSWVNGDVITCQLTGSATCGISTVNSNSVTL
                     TVTSPQTPSVSISASSTSICSGTSVTFTASPVNGGSTPTYQWKKNGSNVGTNSATYIN
                     SSLVNGDIISLTMTSALACVTQSSANSNSITMTVNSSVTPSVSISTAQTSICSGTQVT
                     FTATPTNGGSAPTYQWKKNGLNAASTTSSFTTSLLNNGDVISCVMTGNASCGSGTVTS
                     NLITMTVTQTVTPSISISTFIPSTCSGLALSISANVQNGGSSPVLNWMINGNPTFTGP
                     SPFSWSNYQDGDVVSCQLTSNATCASSTTANSNNITISITETVTPAISISSSTLSSCE
                     NAPVDFTASYQNGGTTPSFLWKVNGSPSGTNSAQFSSSSLANGDVVTCQLTSSFSCVT
                     SSTATSNSLTIAVTPSITPSISISSNATQICSGETVQFTAVATNAGSSPSYAWLLNGN
                     NTGITASNYDNFNLQDNDVVSCQISGNENCIGTAVSTGITMDVTTLITLTINEVGGLL
                     ICDEIPGAIYTWMFNTDIIPDAVNSSFQPTQSGLYSVVYELNDCQSATSAEFNYLLLN
                     VHAVQSSEFTVIPNPASQLVEVKSSSAIDWIIIRDAQGREVLKSSQTINNVSHLASGI
                     YWIQAQNGYELKSAKLVIVR"
     gene            5471..7396
                     /locus_tag="PK281_00835"
     CDS             5471..7396
                     /locus_tag="PK281_00835"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOY47910.1"
                     /translation="MSIQTLLKFCVFVMMSLVLSFPVKAQSKAFLDSLTQALQQSKSS
                     CDKAKTYNELVRAYVSVDLKKAGDFASQGIHFAKTQHCQKELGNLYNSMGIVCITEGN
                     NNQAFAFIDSSLEIHRKLNDKVGIAAALGNKGTFKIYVGDYTDALQLQLECLRIYEEL
                     KDTSAIATTLTNVFGVHYARKDYPHSLETGYQAFSMFQKLKEKDGEALMCFNLASVYL
                     DMKNLDSCIHYINQSDVLFRQIENKEGIADCYRMRSDVKREQHEYAESVALINRAILT
                     YDSIGSVFKQVQSFSYLASVYYDMKLYEKSIVAAEQFLNLSHKYQYKQFERDAAKIMM
                     NNYRALGQYEKAFQYNDLFMKLKDEVMNEQSISEINRLKSDYEFEMKEKEVISVNQQK
                     QILEYELNRKNGLIVGILLLVLLISTIAFFLYQQKKLKSESKTVQLEQRLLRSQMNPH
                     FIFNSLTAIQSFVYKSEPKEAGKFLSSFATLIRTILDNSREEQLSLSKELHWMENYLK
                     LQLLRFENRFSYTIETDENIDLDHTLIPPMLIQPFIENALEHGFRDIDYPGEIHISFK
                     SDGTTLLVSVSDNGLGYSNTISNHPPDHVSHATNITQERLHLLNSNRNQPIKFSISDL
                     PERGTRVEFSIPLIHVR"
     gene            7393..8142
                     /locus_tag="PK281_00840"
     CDS             7393..8142
                     /locus_tag="PK281_00840"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOY47911.1"
                     /translation="MNILVVDDEKRARESVVEVLRMYSKGLRNVYEADGVKSAKEIIG
                     KTSLDLVLLDIHLKDGSGFDLIQDIQAANLPVIFITAHQDYAIRAFKASALNYLLKPI
                     DPDELLHSVTKASILRQQAGFEEKLSVLLEQSMHKNEPPRRIVLRTAESIFVVLVEDI
                     IYCEADKNYTTFHISNRPPILVSGNLGEYEELLSDPRFLRIHQSVLLNLHYIERYEKG
                     DGGFVITRSGQQLPVSSRKKDQLMTYLNNIA"
     gene            8185..8967
                     /locus_tag="PK281_00845"
     CDS             8185..8967
                     /locus_tag="PK281_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY47912.1"
                     /translation="MFSLFKRTKIGCPFCLHQDATELYLSEEERQAYPLPKIFTGNTK
                     KTRIKVSLNIDWTVIYSCTCCKTSFYTVVSHWTKVTDSQKELLKRWGTTSTEIPQEIA
                     AELAKIGFSNWGNDDEILPCKIFTRTSEEIDFVTVQYCDDFPRILCFGDMFVYQKYFF
                     IEDIQRIEASTFAVSKSIRDESVSIKEEVEKNFYPTIVANPAGKEFLFEGMTLFMNHP
                     DAKGSELHLSENKWQKRKQYINDYTPQRQHLLILKKGKKGLK"
     gene            9062..10516
                     /gene="bchE"
                     /locus_tag="PK281_00850"
     CDS             9062..10516
                     /gene="bchE"
                     /locus_tag="PK281_00850"
                     /inference="COORDINATES: protein motif:HMM:TIGR02026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-protoporphyrin IX monomethyl ester
                     anaerobic oxidative cyclase"
                     /protein_id="HOY47913.1"
                     /translation="MYLLIINPPHLSIGSRLAHEHLPPLGLLSIAGPLIDDGHQVELL
                     DADFHNTPISSIVEEVLKHKPDAILLGHSGSTSAQPIINEITSGIRSKAPEIKCIIGG
                     VFPTYHWEEILSTQAQIDYIVCGEGEATCLALIQALENNSDLELVKGIAFRNKGEIHI
                     TPNAETLASLDDYRIAWELMKGYNYTYWGKRKAVVIQFSRGCPYPCTYCGQSLFWKKW
                     RHRDPQKLADEIEMLHREYGIEVINFADENPSSNPKAWRAFLEALVAKNLNLILVGSI
                     RADNIVRDAEFLHLYKQAGFERFLLGIENYDEVVLEKIKKAGTISKDKEAIQLLRKHG
                     ILSMATYVVGFGEEKTRDFYNSLKQLLSYDPDQVQLLYVTPHKWTPYFEEVKDKEIIL
                     TDQRKWDYKHQVISTRYLAPWLVIIYVKLIEIIMQTRPIAIYRLLFHKEARLRSAMRW
                     YTRIGRRVWFWELYQFFFVTRLSKEKMKMGEFWK"
     gene            10750..11145
                     /locus_tag="PK281_00855"
     CDS             10750..11145
                     /locus_tag="PK281_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY47914.1"
                     /translation="MNSTLKLIHWAPRIISILAICFIGMFATDAFNSHESFTNQLKDF
                     FIHLIPTFILLAILLLAWKKELVGGIIFATLGLGLAPFIYQHNYTLNHSILTSLSVIL
                     VINGPFILTGFLFILSHFTKKKNTAFYSN"
     gene            11206..12171
                     /locus_tag="PK281_00860"
     CDS             11206..12171
                     /locus_tag="PK281_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006989857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HOY47915.1"
                     /translation="MKAAIHKVYGPPEVVTIQDVPIPEIGEDEVLIRVHYSTVNRTDC
                     GFRSAEYFISRFFSGLLKPKQQILGNEFSGQIVSIGSLVTKFKIGDRVFGYNDQKFGT
                     HAEFMSMSANSTLALIPESISFAEAAPTLEGGHYAWCDILAAKVKPGQQVLVYGASGA
                     IGSAAVQLLSYLNAKVIAVCGAENIEIVKKLGAIQVIDYKTTDYTTLGVQFDFVFDAV
                     GKTSFGKCKPVLTKDGVYISTELGKNGQNIWLALLSKIKKGKRVLFPLPTINNQDVEF
                     FRELIETGKFKPVIDRTFSLDEIVNAYKYVETGQKVGNVLIKVFD"
     gene            12301..12711
                     /locus_tag="PK281_00865"
     CDS             12301..12711
                     /locus_tag="PK281_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HOY47916.1"
                     /translation="MNPITVQVQINSSIQKVWQFWTDPEKVMLWNFASDDWCCPAAKN
                     DLREGGEFNFTMAAKDGSMQFDFCGTYTSVNTLHHISYILGDGRTVQIDFKEKDGLVE
                     LTETFDPESVNSLELQRSGWQAILNNFKVQVEKA"
     gene            12954..13394
                     /locus_tag="PK281_00870"
     CDS             12954..13394
                     /locus_tag="PK281_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007648200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY47917.1"
                     /translation="MRNSLTLFLLMCLIVTTQTACKRDDNSSNDQSVPSQVLNNVQQG
                     NWIITLFNDSGSDELYHFTGKTFAFSNGTVTTTHNGATISGTYSSGTDDSMNKFLLNF
                     GSVSPFDELNDDWHIIEQTKTKLRLQDVSGGNGGTDLLIFEKAN"
     gene            complement(13551..14249)
                     /locus_tag="PK281_00875"
     CDS             complement(13551..14249)
                     /locus_tag="PK281_00875"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOY47918.1"
                     /translation="MKKILLFLGICCSFSIVSAQDSTKTNVTINTNSSTTTNNLPTDN
                     REKYQFGLKAGMCIADVFAGQGSQFHAESKYALTAGAMVHFPITKYFGFQPEISVSQK
                     GFKGSGELLASSYNFTRTSFIMDIPLQMVFKPTEFISLLTGFQYSYLIKQRNEFTTSF
                     SSYSQEQVFRNETLRRSNAGFICGADINLKHMVLGARASWDFFRNTTDDSSLTPRYKN
                     VCFLGTVGYKFYTD"
     gene            complement(14397..16043)
                     /locus_tag="PK281_00880"
     CDS             complement(14397..16043)
                     /locus_tag="PK281_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY47919.1"
                     /translation="MKEWLKKYICHRTKLFVVGVILILTGLMIHLLIINSIRNKLSLL
                     VWKSSAGKYELNLAEIKINYLSRSIFLNHVECFPKQGLKTKGDLIYSRFSSIELAHID
                     LWDLTFHDKIKINEINFNHPLIVYFKADSIEHNPIKKPDEKLSIPYSVEVGGISFSSF
                     PFYWMENKQNPRLILISQGSTLAIHDFKLEQLGLNNQLDVSVGPTTLKIPSFSFPQVN
                     KLYRIVGNNLAYEGIDSTLSIEAIHLIPLYNTKDFADSARMQITRMDIRSFEWIVNGF
                     SLNTLFQEQELRIKTLKIRNAKIDAYRNNQFAGAIRYCASMQDLIRQIPFKLRVDTIQ
                     ADSIFVRFRVLAPKSRLPGSIFLTQMNVRALGIYTINQQQVLQPFKIDMSGLVMNQGK
                     LKIQFEFPFDSSQNVFYCKGSLGKMPFVSFNTLLENSKGIRIKAGIIDSVSFWFKADE
                     KYSNGKMNFQYHNLSIENLKVNPKESLMHLRLRSLLLNNFIAIAENPSNSNQLRISPI
                     YAKRDTSRYFLHYASQSIMSGIIHSLIGEKKMKLQKQLLK"
     gene            complement(16189..17538)
                     /locus_tag="PK281_00885"
     CDS             complement(16189..17538)
                     /locus_tag="PK281_00885"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HOY47920.1"
                     /translation="MNKQLLFIATCIITPGLALAQWNTVASMSTSRAGHAMTAYGEGH
                     VLAAGGWDYTTNLKSAEYYDASSNSWTSTPDMASEHYNGAAVTLNSGKVLVIAGFTGA
                     FNTEACDLYDPTMNMWAATGELTQGRSYFTATKLQNGKVLVVGGFDGTNNLSSCELYD
                     PTTEEWTTIASLTTGRSYHTATLLSSGQVLIAGGFNPNAGFQLNSTELFDPTNDTWSP
                     GPNMSIGRDFHAASILANGKVLVTGGRYFNGSINFAYNGLTDTELYDPTTNSWSAASA
                     LPVGLSYHQQVTLGDGNVLVIAGVDSSNYSTGSGFTTFASSTYLYHSTMDMWVAASMN
                     HDSRYEHAAVVMSDGNALVTGGFDASSEIYGTIAGIEIATTKPVMNMYPNPGKGLIRL
                     DLEKADQTIRVFDSMGKLVNIPMEKTANGFILQTAGIQAGCYIVLAGSEKQTLVVLN"
     gene            17757..18317
                     /locus_tag="PK281_00890"
     CDS             17757..18317
                     /locus_tag="PK281_00890"
                     /inference="COORDINATES: protein motif:HMM:NF019614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HOY47921.1"
                     /translation="MKTYISILRGINVSGQKMIKMESLRELYSGMGFQRIQTYIQSGN
                     VVFQTDDADVDYLQVNIQNNISEIFGFDVPVMVFEFDQFKQMLNQNPFVNDSEKEVNH
                     LHVTFLSAIPETERVAKMDEFDYQNDRFQLIDKAVFLYCPNNYGNTKLSNNFLETKLK
                     VKATTRNWKTSQELIRIAEAISMQED"
     gene            18383..19102
                     /locus_tag="PK281_00895"
     CDS             18383..19102
                     /locus_tag="PK281_00895"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY47922.1"
                     /translation="MKFLKQSPLKLTLFFALNLFFLVENTQAQALLNSEWRSSGTFQT
                     VLQSNFFVDSVYMKFLSNDEFQFVYFEGSGQTIAKGLWYQDDNSHFRFVDTSETYLGL
                     PCPQDTFSIVYSVSGNTLQFDQVENGCTTVSSILIGSSWINTDLTAGINVLNFNKDKA
                     ICFPNPAQGGIFYHLNQDSNLTDGLTLEVYNSVGQSILKDVNSNLNGRINLEKVPVGL
                     YFIRFSNHAKSINWVESIILQ"
     gene            complement(19189..20541)
                     /locus_tag="PK281_00900"
     CDS             complement(19189..20541)
                     /locus_tag="PK281_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOY47923.1"
                     /translation="MGPRTENRKLSFRQIWNMSFGFLGIQFGFALQNGNASSILQNFG
                     AHVEELSWFWLVAPFTGMIVQPIIGHYSDRTWNRLGRRKPYFLVGTLICCCALLLLPN
                     SGVFFVGKMALFIGAMFLMLMDASINVAMEPFRALVADNLPDNQRTLGFSIQTFLIGV
                     GAVVGSWLPWILKNWFDVSASAPAGQVADNVIYSFYFGAAVFLFAILVTIIRTKEYPP
                     AEYAEIHGESEVEKAGLNQIFKDFAKMPKTMRQLGLVQFFSWFALFSMWVFTTPAIAQ
                     HIYHLPIEDTSSDQFRDAGSWVGIIFGVYNLVSALYALLLPSIAARFGRRNTHAFSLV
                     CGGLGLISMYFAPNKEFLIFSMIGVGLAWASILAMPYAILAGSIPAGKMGVYMGIFNF
                     FITIPQIVNGIFGGPIVKHFYNSEAIYAIILAGIFMILGAISSFYVQDATTINMNDKR
                     "
     gene            complement(20937..21926)
                     /locus_tag="PK281_00905"
     CDS             complement(20937..21926)
                     /locus_tag="PK281_00905"
                     /inference="COORDINATES: protein motif:HMM:TIGR01840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HOY47924.1"
                     /translation="MTFNQQSRNPLLFFNLLMVVCFLLISNYLSAQIEQVEKFGSNPG
                     ELRCYIYKPKTLLNSDAPLLMVLHGCSQTAEEVSNQSGWNKLADSLGMLVVYPQQHLM
                     NNPSRCFNWFRKEDITRGGGEVESLHQMIQYCMAHYPIDNKRVFVFGLSAGAVMGTIL
                     MAAYPEEITAGCSLAGPAIGTWEGSLSQMKDLYDTDEYVPQSQLEKLRACYPGYTGTY
                     PLLLTVQGSNDVLVTIEDQTKQITQWTAANGSDAVPDKIELINNDPNVERSVYTNSQG
                     RTVVIAYQISGMGHVLPINTGNRIDEGGKTGLFSKELNFHLPYWVMREFGMVK"
     gene            22039..24684
                     /locus_tag="PK281_00910"
     CDS             22039..24684
                     /locus_tag="PK281_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008990601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase/topoisomerase IV subunit A"
                     /protein_id="HOY47925.1"
                     /translation="MAEETPEDLNEQLESPENGGSEERITHVSGMYENWFLDYASYVI
                     LERAVPYIEDGLKPVQRRILHAMKELDDGRYNKVANVIGHTMKYHPHGDASIGDALVQ
                     LGQKDLLIDCQGNWGNIYTGDSAAAPRYIEARLSKLAGEILFNAKTTHWQLSYDGRNK
                     EPITLPVKFPLLLAQGVEGIAVGLACKILPHNFIELIDASISVLKKKPFEIYPDFVTG
                     GMADFTKYNDGARGGKVRIRAKISQLDKNTLVITDLPFGTTTGSLIDSILAANDKEKI
                     KIKKIEDNTSDVVEILVHLPPGISPDKTIDALYAITSCEISVSPNAVIIENDKPRFVG
                     VSEILRLSVENTVDLLRRELEIEKSELEEQWQFSSLEKIFIEERIYRDIEECETWEAV
                     IEAIDKGLKPFRKKLRRDVTHEDIVRLTEIRIKRISKFDAFKADEYIKGLEDKIANCE
                     HHLANLTDYAIAWYQNLKKKYGTGRERKTEIRMFDTIEASKVVINNEKLYVNREEGFA
                     GYGIKKDEYVCECSDIDDIVVMRADGKMIISKISDKAFVGKDILHVNVFRKNDDRTTY
                     NVVYQDGKTGPYYVKRFAISGVIRDKEYDMTKGTKGSRVVYLSANPNGEVETLQIFLK
                     PRPKLKKTVFEFDFGTLAIKGRGAGGNIMTKIPIRKIILKEKGQSTLSARQIWWDDAV
                     KRLNTDGRGDLLGSFAAGDKILALMQSGTYKLYEPDPSTHFDEDLIQLGFFDQRQPVS
                     AIYYDGESKEYMVKRFLIETTDKKTTFISEHPNSRLEFVSTDTFPRVQLEFGKMGKFE
                     RNAQEILLEEFIAVKGLKAKGKRLSTHEPKKIIALESLPEPELEEVEEIEHEDSDSEQ
                     TPSDKPNVIIDLEGDEPQMRLFEEP"
     gene            24843..26060
                     /locus_tag="PK281_00915"
     CDS             24843..26060
                     /locus_tag="PK281_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY47926.1"
                     /translation="MKRISSLLLALCSLGASAQIITREDSLHAGLTPSTNPTVISGYG
                     QFKAEYDTRYQTAKASLTRNVLFFGHKFNKRIAFFSEMELEDAKVVGGSPSGEISMEQ
                     MFLKFGINNDIYLTAGLFIPRIGLVNENHLPTIFNGNDRPFVERLLIPSTWRELGVSL
                     YGRSRRIPGLNYSAGIMNGLNSQGFGNGEGIREGRFEGNNATAGNLAVTGALLYYRQG
                     FRFQASAYYGGSAGLVKRVADSLQLDSRPFGTPVFLTEANVQYQSQSGFEFKVLGTLS
                     TIKDAASINRAYSNNTPGKMWGAYAEIGYNILKAFKPNTERSLVLFARAEAMDLNAEV
                     PFNGIHNPEQNKQFFITGITYTPVRGVVVKVDYVHRMTGTPNPALILTPYPSALPYFN
                     SNGFLNIGAGYSF"
     gene            26063..26491
                     /locus_tag="PK281_00920"
     CDS             26063..26491
                     /locus_tag="PK281_00920"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HOY47927.1"
                     /translation="MNRRNFIGKTARLGACSLIGVSLLTHSGCASYPLVKVKPVNNLL
                     KVDLTAFGEGKRLLVRTPDLEFDVFVHQFQTGQFRATLLQCSHRNEPISVSDTQIYCP
                     SHGSTFSLEGAVTKAPATEPLKHYPVKLSDDGTSLLIDIS"
     gene            26517..27593
                     /locus_tag="PK281_00925"
     CDS             26517..27593
                     /locus_tag="PK281_00925"
                     /inference="COORDINATES: protein motif:HMM:NF020932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imelysin family protein"
                     /protein_id="HOY47928.1"
                     /translation="MLLPNKFLLTSLTVLALVACNKKDDTPELSDTFASRKVILSDIS
                     QHVIAATYADMQTQANTLNTEITELQTSTTDANLTECRNSWRNVRSAWEQSEGFLFGP
                     VASENIDPRIDTWPVNHIDLDSILASSVEFTPQYIDELQDALKGFHPVEYLLFGLNGD
                     KTAAQFTDRELSFLKALADNLQILCSQVSGRWNSSSSDSYLSKFVNAGSGSDEYPTQL
                     AAFEEVVNAMIGICEEVAGGKISEPFAAQDPSLEESPYAQNSLKDFISNMKSVKNIYY
                     GSYKTSGQGIKDYVRLYNLSLDARIKQDIDQAIAALEAISDPFGLAISTQGTLVQQAI
                     TRIETLKETLETQLLPLIQQHIKE"
     gene            27596..28981
                     /locus_tag="PK281_00930"
     CDS             27596..28981
                     /locus_tag="PK281_00930"
                     /inference="COORDINATES: protein motif:HMM:NF018269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HOY47929.1"
                     /translation="MIRVKVHSVPFFTLLLLGSFASCTSKEDFPETSHNEYYAGGNQT
                     AFISGAGAFSSAFPVLNASQSRVHEIGDIGFEATFVSGSQVDLGGLGPVYNNVSCGSC
                     HIADGRGKAPEAGESLASLLIRISVPGTNEHGGPLGVPGYGGQFQPRSIYGVAKEGDV
                     NYSYEYLHGSFADGETYELRQPVIELNNTYSTLPSDVMLSPRLASPVFGLGLLEAIPE
                     SSILAHEDALDANNDGISGKANRVWEVQKNRFSLGRFGWKAGQPSVIQQSAGAYVEDM
                     GITNTIFTLESVYGQPQQNAGNTHVELSDSTLHAVAFYVQTLAVPARRDVEKPQVQLG
                     KTLFTQIGCANCHTPQQRTGVDVVFRAVSNQVIFPYTDLLLHDMGEELADNRPEFDAD
                     GKEWRTAPLWGIGLTGVVNGHTNFLHDGRARNFTEAILWHGGEGNNSRQAFKNLASND
                     RQALLAFLKSL"
     gene            complement(29136..31364)
                     /locus_tag="PK281_00935"
     CDS             complement(29136..31364)
                     /locus_tag="PK281_00935"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOY47930.1"
                     /translation="MKTQLMLFLMLCALEGTSQTINGQVLEENDEGITLPLTGVSVYW
                     LGTNIAVQSDSLGNFQIADEHLHSKLILSFTGYRPDTLTLTGSEFVRVTLHQRTQLRT
                     LEIAGKQSSTYLSKLGAIKTEVITTAELCKAACCNLSESFETNNSIDVSTTDAVTGTK
                     KITMLGLSGIYTQITQENLPAMRGLASSSGLQQLPGTWIESIQISKGVGSVANGFESI
                     TGQINLELKKPFTDETLLLNGYVNQMGRMEVNLSKAIALNEKWGTVLLVHGDQMKKPI
                     DRNKDKFMDVTDGNQWNILNRWMYRSGKQLSGQFGIQALGDNRRAGTAHQGNEFDVLH
                     DWRFNKDVLRFDAFGKLGYIFQGRSLTSIGILGNAQMNQQRTILGNRWYDADQQSAYG
                     SLIFQSFIGNSNHTYRTGFNVQYDSMKEYFTGQPFGRKELVAGAFFEYTWKSGMSTLV
                     AGIRADHNFLYGNFATPRLHYKLAFSEKTALHVSGGRGQRTATILDENISYLVSSRLL
                     LVKANAAAQGKGYGLLPEIGWTGGIGFSTQFELAGQEGSLSADAYMTWFERQVVVDAD
                     FSPQQLRIYNLDGNSQSVSTQVDYNQALGKRFDLRLSYKFTQTQTQYLSGTFQRPFVS
                     PHRALLNLAFHSRKDKWLVDATANFIGSKRLPSSVLNPVAYQADSHSPDYVMVHAQVT
                     RNLNRWAVYVGVENLLDVYQQNQIISSDDPHSKYFDASYSWGPGFGRMVYAGFRYKIM
                     KS"
     gene            complement(31399..31758)
                     /locus_tag="PK281_00940"
     CDS             complement(31399..31758)
                     /locus_tag="PK281_00940"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HOY47931.1"
                     /translation="MKSLFLSGIFALFIFPVFAQTTSTILVAGNCDMCKKRIESAVDL
                     KGVKKASWDAKTKVLTVNYDPKKITLDKIIEEISLAGHDSEWLKADSAAYEKLHNCCH
                     YERMEPSIPEPKMLDSY"
     gene            complement(31808..32191)
                     /locus_tag="PK281_00945"
     CDS             complement(31808..32191)
                     /locus_tag="PK281_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY47932.1"
                     /translation="MLNRLLLFPILFLYLFSTVGIRMQAHYCHGEFSSVSLNPFHQLS
                     CACESEVEESNCCTDAQFLLQVKDSYANSTSVSIPNLQAFTLLSECYSLINITSTSDK
                     VQLVDNRKVDLPPPDTLVGYGQWRI"
     gene            complement(32193..32414)
                     /locus_tag="PK281_00950"
     CDS             complement(32193..32414)
                     /locus_tag="PK281_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019973643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HOY47933.1"
                     /translation="MKTLEFSTSINCGSCVRAVTPTLDALVGANNWKVNTEDARKILS
                     VYLENMDAGQICDALKSIGYSAEPLQVQV"
     gene            32498..34132
                     /locus_tag="PK281_00955"
     CDS             32498..34132
                     /locus_tag="PK281_00955"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOY47934.1"
                     /translation="MKHKNRISKASIFLLLVLIQLPTFSFSQNARKFRVFFADKPEQN
                     FDPYSYFDPATIQRRKQQNLPIYDWYDLPVAQSYLNQIGQNCERIKMHSRWLNAAVIM
                     ANDEQIEKIRSFSYVKTIEEADPMQIQLASIDEVEQNLQQSDSNEDEVLNTQIKRMGA
                     DLLAEKGLTGKGIRIAIFDVGFSGADKHTAFQHIRSRNGVFKTWDFVKNEENVWKGGW
                     HGTAVWSCIAGKLKGRWSGLATDADFLLARTEIGSKEPFAEEEYWVAAAEWADKNGAD
                     IINSSLGYTDSRYFPRQMDGKTTFITRGANLAAKKGLLVVNAAGNEGDSNWRIIAAPA
                     DADSVLTIGGVEPCCDYHISFSSYGPTRDMRLKPNVCAQGRAFCAKENGESNADGTSF
                     ASPLAAGFAACAWQAKRSLSNMQLFDELQKCASLYPYFDYAHGYGVLSADRFLNSIVA
                     TPTFVAKKANITSETEDSGEYIQIEVDDKYTTVNPEGMDYLLYYSIVNTSGGLDSYTV
                     IKVHEKIPVQFKTKAYSTGTKLRISFQGYMSEIQFQ"
     gene            34132..34830
                     /locus_tag="PK281_00960"
     CDS             34132..34830
                     /locus_tag="PK281_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY47935.1"
                     /translation="MKKLLFSFAVIIAGMNQLAAQDVVFKMDPAVDTTESKSGPNLKN
                     YWHPFMSYGFCIDPGSAGATVKQPHLDQFSFGYRYKNRLAEHLAVGLDISYNVNDYSL
                     KQNASKTLPDTVQYKKQRMIFYNAQVGAYVRINFGKRGNTLGNYLDLGGYGDYVMSHV
                     QFTKFNLPDGSIVRARRTQLDYYQKINYGLLARIGFKKLVIYGQYRISDMFYPSLKMA
                     ELPRIQAGIQFVLR"
     gene            34836..35975
                     /locus_tag="PK281_00965"
     CDS             34836..35975
                     /locus_tag="PK281_00965"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HOY47936.1"
                     /translation="MRIAVNTRLLQSGKLEGIGWFTFETLRRITLAHPEHEFLFLFDR
                     PFSEEFVFAPNVRAVYAGPPTRHIFLFFPWFEWQIPRLLKKHQVDLFLSPDGTLSLRS
                     DVPQIAVIHDLNFHHHPEQLPRIVRWYYNTFFPRYAAKAARIATVSEYTKQDIIKSYG
                     IAPNKIDVTWNGYNTRYKPLGDEINDQTRAQYTNGKSYFLFVGLIIPRKNLIRLMLAF
                     DRFKEKSKSDVQLLVVGEKKWWDQEHENAFQNLQFKNEIHFAGRLQPEELHKVLSAAQ
                     ALTFVPTFEGFGIPILEAFSAGIPVITSNVTSMPEVAGNAALLANPFDVDDITEKMIE
                     LNQSPELQKVLIEKGNKRKEEFSWDKTAQLLWNCIQKEIDYINRS"
BASE COUNT        11447 a         7008 c         6707 g        10879 t
ORIGIN      
        1 aaaacttcac cagcacttta gttgcttaca acaacttaca catggacaca accgacctcg
       61 attcggatgg tgataaagac ttgatttcag gtggattata ttgtctaagt tgggaggaaa
      121 atcgcggtaa cggattattc gtcaaccacg ttatatcgca gcttcagcct gaagttcaag
      181 cagttgttgg tatcgacttg gatcaggatg gttatcaaga tatcatctca gcatcgttga
      241 gtaataatgc tatctattgg aaccgcaata atggaatgca aacttttagt agcaccacat
      301 taaattcgac tgctgctggt cctgctggat tagctgctgc cgattttgat aatgatggag
      361 atattgattt ggccgttgct tcttatacgg gaaataaaat attctggcta cgtaataacg
      421 gttcacaaag cttcactcaa gttgatttac tcagcaattt caacggtcct accaaagtca
      481 gaacgggcga tatggatggt gatggtgatc aagatatttt agcttctgcc cgcacaggaa
      541 atcaaattgt ttggctgaga aataatggat caggtgtttt ttctaccgtt aatatctcca
      601 atagccttac agcgcctcgt gaagtaaaac taagagatta cgatcaagat ggtgatttag
      661 atattattta tgcttccgat ttaggttggg gttggtttag aaatgatgca accattttta
      721 atattttccc ttattctaca ggcacaggaa tttattctgt tgaattagct gatctcaata
      781 atgacggaca tctcgacttc gtcacagcaa attattcaag tgaattaatc aactatagtt
      841 tgaattcagg gaaccaaaca tttagctcgg gtggaaccat cgataatcgt acttcttacc
      901 ccaatcaatt agtttgtgct gattataatt cggatggaaa agtagatgta atgtgcggcg
      961 gaggattaga tattcgttat tgtcaaaaca gcgccacgct tagctttacg gctaaacaag
     1021 tcatgcaata tgcaggaaat aatcatggtg cttgtcatgg tgattttgat aatgatggtg
     1081 atgtggattt aatggaagtt ggcttcacgt ttatgtattg gtatcgtaac gagagtaatg
     1141 gcgagttcac cccaattcag ctccaagaca atggtttctc ctacataact accaattatg
     1201 gagtcaatat tcgtactgca gatatggatg gagatggtga tgaggatgcg atttataccg
     1261 aaaatgacaa caataaaatt agctggattg aaaataaagg aaatggatta tttgcctatc
     1321 attttgcagc aagcgtgatg gggccataca gcctcgaacc cattgatttt gatgatgatg
     1381 gcgatatgga ttttgtcagt ggaagtactg ctgatgatgc ggtttattgg tacgaaaata
     1441 atggcagcca aaccttcacc gaacacatgg tgagtggaat gtattgggat ccattttcgg
     1501 tgcgtgtttt tgattacgat caagatggtg atatggatgt tttttccgca caaggaaatc
     1561 caaccaataa attaattgta ttcgataatg atggaaataa cctcgatttc accgattata
     1621 tcatcgataa ctcggcacct ggaattaatt caatcgatgt aaaagatgta aatggtgatg
     1681 gtgatttgga tttattgagt gcatcatcga ccgacgataa aatttcttgg tacgaaagct
     1741 caggtggaag ttttccttca ttcactaaaa aaacggtaag tactggcgtg gatggtgcca
     1801 cttatgtttt tggtggagat tataatggtg atggtgaaat ggatgtcatt tctacttcaa
     1861 ccgtcgataa aaaagtaagc gtttatataa atgatggatc tcaaagtttt aatcgcatca
     1921 atattggaaa tcaggttgat ggtgccgact ttgttgaatc gggcgattta gatggagatg
     1981 gattagaaga aatttatgca actggatcca cacacggaat tgtacagatt tttaaatcta
     2041 caccattcgt attaccacca gccgttgatt taactccatg tggtgatatt tttatttcgg
     2101 aatatgtgga aggctcatcc tataacaaag ccatcgaaat ctataatccg actcccaatc
     2161 ctattgattt agcaccatac cggattttat tttatgccaa tggagctacc actgcgactc
     2221 aaactattgt cccttcaggt attttacaac ccaacgatgt atggattata gcacattctt
     2281 tatcagatct cgaatttttc cttgcagccg atattgatgc aacgttgaac ttcaatggtg
     2341 atgatgccat cgcattaact aaaaatggag atataatcga tttaattgga gtaatcggac
     2401 aagatcccgg aactgcatgg acaggcactt ctggagcttc cacactcgat aaaacactcg
     2461 tgcgtaaacc cgaaatcacc aaaggaaata acattcaggg ttctagtttc gatgcttcgg
     2521 ttgaatggat tgtttaccca atagatgcct atcaatatat tggaaatcac accaatgttt
     2581 gtaatggcgc ctgcattcca agcataagca ttagtgccag ctcaactgat atttgtgcag
     2641 gaacatcggt tacgttcaca gcaagcgtga caaatgaagg ggctgctcct acctatcaat
     2701 ggaagaaaaa tggttcagtt gtcggaacaa atgctagcac attcagtact agctcactcg
     2761 ccgataatga tatcatcacc tgcgaattaa caagcaatgc tgcctgtgct attccgggag
     2821 ctatttcatc caattccatt gtgatgaatg tgaatgtttc ttctgcttcc agcattagta
     2881 ttagcgcttc ggctacgagc atttgtgcgg gaacatcagt cacctttact gcaacaccaa
     2941 ttaatggtgg aacaacacct atttatcaat ggaagaaaaa tggtggaaat gtgggaacga
     3001 actcaagtac cttcaatgtg agcacacttg ttaatggtga cattattacc tgcgttttaa
     3061 ctagtaatgc ggcatgtgtg agtaatacat catcaacgag taatgctatc acgatgattg
     3121 tccaatcaag cgtcacacca agcattagca tcacggcatc gacaacaaca atttgtgctg
     3181 gaacttcggt tacattcaca acaaatgcag taaatggcgg aacaacacct agctttcaat
     3241 ggaagaaaaa tggtgggaat gtgggtacta attcaactac ctatagcagc tctacattag
     3301 tcaatggaga tatcattaca tgtgaactta ctagcaatgc aacttgcgtt tcaagtgcgg
     3361 tagttagcag taattcgatc agcatcatcg tcaacccaat cgttacacca agcatcagca
     3421 tttctgctag tcaaactaca atctgtgctt ctaccagtgt gacattcacc gcaacgccag
     3481 taaatgctgg tagctcacct tcataccaat ggaagaaaaa tggtggaaat gtggggacaa
     3541 attcagccac ctatactgcc tcttcttggg tgaatggaga tgtgattacg tgtcagctta
     3601 caggcagcgc aacctgcgga attagtacag taaatagtaa ttcagttaca ctcacagtaa
     3661 cttccccaca aacaccatca gtgagtattt cggcaagtag tacaagcatt tgcagtggca
     3721 cttcagttac tttcaccgct agtcctgtaa atggtggttc aactccaact tatcaatgga
     3781 aaaagaatgg ttctaacgta ggaacaaatt cggcaaccta tatcaactct agcttggtca
     3841 atggtgatat tatcagccta accatgacaa gcgctcttgc atgcgtaacg cagagttcag
     3901 caaatagtaa tagcatcacc atgacagtta attcatctgt aacaccatcg gtgagtatta
     3961 gcacggctca aacaagcatc tgctctggaa cacaagtcac ttttactgca acaccaacaa
     4021 atggaggtag tgcaccaact tatcaatgga aaaagaatgg attaaatgca gctagcacca
     4081 catcttcgtt tacaacgagc cttttaaata atggtgatgt gatatcttgt gtaatgacag
     4141 gtaatgcatc atgcggctct gggacagtta ccagtaatct tatcacaatg actgtaactc
     4201 aaacagtaac accatctatt tccataagta cattcattcc ttcaacctgc agtggattag
     4261 cattgtctat atctgctaat gttcaaaacg gaggaagttc acctgtttta aattggatga
     4321 ttaatggaaa tccaactttc actggacctt caccttttag ttggtcgaat taccaagatg
     4381 gagatgttgt ttcctgtcaa ttaacaagca atgctacatg tgcaagtagt actacagcga
     4441 atagtaataa cattacaatt tctatcaccg aaacagtaac acctgcaatt agcatttcta
     4501 gtagcacatt gtcgagttgc gaaaatgcac ccgttgattt tactgcgagc tatcaaaatg
     4561 gaggaacaac accgagcttt ctatggaaag taaatggttc tccgagcggg acaaattcag
     4621 cacaattcag cagttcttca ttagccaatg gtgatgttgt tacgtgtcaa ttaacgagtt
     4681 catttagctg tgtgacaagt tcaacggcta ctagtaattc cttaactatt gcagttacac
     4741 cttcaattac accaagtatt tcaatttcga gtaatgcaac tcaaatttgt tcaggtgaaa
     4801 cagttcaatt tactgctgtt gctaccaatg caggttcatc gccaagttat gcatggttgt
     4861 taaatggaaa taatacaggt attacagcaa gcaattatga caactttaat ttacaagata
     4921 atgatgtggt aagttgtcaa atttcaggaa acgaaaattg cattggaaca gctgtatcta
     4981 caggcattac aatggatgta acgacactca ttactctaac aattaatgaa gttggtggac
     5041 tacttatttg tgatgaaatt ccaggagcaa tatatacgtg gatgttcaat acagatatca
     5101 tccctgatgc agtaaattca agctttcagc ctacacaaag tggtttgtat tcggtggtgt
     5161 atgaattaaa tgattgtcaa tcggcaactt ctgctgaatt taattatttg ttgttaaatg
     5221 ttcatgctgt tcaatcctcc gaatttactg tgatacctaa tcctgcaagt cagttggtcg
     5281 aagtaaaaag tagttcagca attgattgga ttattattcg tgatgctcaa gggagagaag
     5341 tattaaaatc atctcaaacg attaataatg tgagccattt agcttcggga atttactgga
     5401 tacaagcaca aaacggttat gaattgaaaa gtgctaaatt ggtcattgta cgttaatctg
     5461 aaataagcga atgagcattc aaacgctgct gaaattttgt gtgtttgtga tgatgagtct
     5521 tgttctgtct ttccctgtga aagctcagag caaggctttt cttgatagtt tgactcaagc
     5581 attgcagcaa tcaaaatcat cctgcgataa agcaaaaacc tacaatgaat tagtaagggc
     5641 ttatgtttct gtcgatttaa aaaaagcggg agactttgct tctcaaggaa ttcattttgc
     5701 taaaactcag cattgccaaa aagaattagg gaatttatat aattcgatgg gaattgtatg
     5761 tattactgaa ggcaacaata atcaagcgtt tgcctttatt gatagttctt tagaaataca
     5821 tcgcaaactc aacgataaag taggaatagc ggcagcttta ggaaataaag gaacatttaa
     5881 aatttatgta ggtgattata ccgatgcctt acaattgcaa ttagaatgtt tgcgtattta
     5941 tgaagaatta aaagacacga gcgcaatagc aacaacactt accaatgttt ttggagtgca
     6001 ttatgcacgt aaagattatc cgcattcttt ggaaactggc tatcaagctt tcagtatgtt
     6061 tcaaaaactg aaagaaaaag atggtgaagc cttgatgtgc ttcaatttgg catcggtgta
     6121 tttagacatg aaaaatctcg attcatgtat tcattatata aatcaatcgg atgtattatt
     6181 ccgacaaata gaaaataaag aaggaattgc cgattgctat cgaatgagaa gtgatgttaa
     6241 acgcgagcaa catgaatatg cagaatcggt cgctttaatt aatcgtgcta tacttactta
     6301 tgattcgata ggctctgttt ttaagcaggt tcaatcgttt tcgtatttag cttcggtgta
     6361 ttatgacatg aaattatatg agaaaagtat tgtcgcagca gaacaatttt taaatctatc
     6421 tcataaatac caatacaagc aatttgagcg cgatgcagcc aaaatcatga tgaataatta
     6481 tcgtgcttta ggacaatacg aaaaggcatt tcaatacaac gatttattca tgaagctcaa
     6541 agatgaagtc atgaatgaac aatccatttc ggaaatcaat cgcttaaaaa gtgattacga
     6601 atttgaaatg aaagaaaaag aagtgatttc tgtaaatcaa caaaagcaga ttttggaata
     6661 tgaattaaat cgaaaaaatg gattaattgt cggaattctt ttattggttt tgcttatttc
     6721 aactattgcc ttttttctct atcagcaaaa gaaacttaag agtgaaagca agacggttca
     6781 attggaacag cgcttattac gttctcaaat gaatccgcat tttatcttta attcactcac
     6841 agccattcag agttttgttt ataaaagtga acccaaagaa gcggggaaat ttctatcctc
     6901 attcgccacg ctaatccgca ccattctaga taattcgaga gaagaacaat tatctctttc
     6961 gaaagaatta cattggatgg agaattattt gaaattgcaa ttactccgtt tcgaaaatcg
     7021 tttttcctac acaatcgaaa cggatgaaaa tattgatcta gatcacacgc ttatcccgcc
     7081 aatgctcatt cagccattta ttgaaaatgc tttggaacat ggatttcgtg atattgatta
     7141 tccgggtgaa attcatattt catttaaatc cgatggaaca actttattag tcagtgtgag
     7201 cgataatggg ctcggatatt caaacactat atcgaatcat ccaccagatc atgtttctca
     7261 tgcaactaac ataacccagg agcgtttaca tttgctgaat tccaatcgga atcaacccat
     7321 aaaattcagc attagcgatt tacctgaacg tggtacacga gtagaattca gtattccttt
     7381 aattcatgtg cgatgaatat tttagttgtt gacgatgaga aaagggcacg cgaaagtgtg
     7441 gtggaagtcc tacgtatgta tagtaaaggt ctacgtaatg tgtatgaagc cgatggtgta
     7501 aaaagtgcca aagaaattat aggtaaaaca tctttagatt tagttctact tgatatccac
     7561 ttaaaagatg gtagtggatt tgacctcatt caagatattc aagcagcgaa tcttccagtg
     7621 attttcataa cagctcatca ggattatgcg atacgggcat tcaaagcatc agcactgaat
     7681 tatctgctca aacctatcga tccagacgaa ttattacatt ccgtcaccaa ggcatctatc
     7741 cttcgtcaac aagctggttt tgaagagaag ctctcggttc tgcttgagca atccatgcat
     7801 aaaaatgaac ctccgcgacg aatcgtttta cgcactgctg agagcatttt tgtcgtgttg
     7861 gtggaagata ttatttactg tgaagcagat aaaaactaca ccacatttca tattagcaat
     7921 cgcccgccca ttctcgtttc gggcaattta ggtgaatatg aagaactgct ttccgatcct
     7981 cgatttttac gcatccatca atcggtttta ctcaatcttc attacatcga acggtatgaa
     8041 aaaggcgatg gtggatttgt cataactcgt agtggacagc aattgccggt ttcgagtcgc
     8101 aaaaaagacc aactcatgac ttacttaaat aatatcgctt aacattctag cactttttcg
     8161 attcgcattt gcgatattgc tagcatgttt tcacttttca aacgcactaa aattggttgt
     8221 ccattttgtc tgcaccaaga tgcgactgaa ctttatctaa gtgaagaaga gcgacaggca
     8281 tatcccctac ccaagatttt tacaggaaat acgaaaaaga cgcgcatcaa agtaagtctg
     8341 aacatcgatt ggacagtaat ctatagttgt acttgctgta aaacgtcatt ttatacagtt
     8401 gtttcacatt ggaccaaagt aaccgattca caaaaagagc tattaaaaag atggggaact
     8461 acttccaccg aaattcctca agaaattgct gctgaattag cgaaaatcgg ttttagtaac
     8521 tggggaaatg atgatgaaat attaccctgc aaaattttca ctcgaacaag tgaagaaatt
     8581 gatttcgtga ctgttcaata ctgtgatgat tttcctagaa ttttgtgttt tggagatatg
     8641 ttcgtgtatc aaaagtattt ctttattgaa gacatacagc gcattgaagc gagcactttt
     8701 gctgtctcaa aatccattag ggatgaatca gtgagcatta aagaagaagt agaaaagaat
     8761 ttctatccta ctattgttgc gaatcctgca ggaaaggaat ttctatttga aggcatgact
     8821 ttattcatga atcaccccga tgccaaagga agcgaacttc atctttctga aaataaatgg
     8881 caaaaaagaa aacagtatat caacgattat acaccacaac gtcagcattt attaatcttg
     8941 aaaaaaggaa aaaagggtct aaaatgaaat tcttaaatat tcctaattag tgattttatc
     9001 agcaataagc aatcattcct ctcgtttcac atcggcttaa gaaacagtct caatctataa
     9061 catgtattta ctcattatta atccacctca tttatccatt ggaagtcgct tagcacatga
     9121 acatttacct cctttaggat tattatccat tgcaggtcca ttgatagatg acggacatca
     9181 ggttgaatta ttagatgccg attttcacaa tactcccatc tcctccattg ttgaagaagt
     9241 attaaagcac aagccagatg cgattctttt aggacattcg ggttccactt ctgctcaacc
     9301 tatcatcaat gaaataactt caggtattcg ttcaaaagcg cccgaaataa aatgcatcat
     9361 tggaggcgtt ttccctacct atcattggga agaaattcta tccactcaag ctcaaattga
     9421 ttatatcgtt tgtggtgaag gggaagcaac ttgtcttgca ttaattcaag ctttggaaaa
     9481 taattcagac ctcgaattag tgaaaggaat tgcctttaga aataaaggag aaattcatat
     9541 aacacccaat gccgaaaccc ttgcaagtct ggatgattac cgcattgctt gggaattaat
     9601 gaagggctac aattacacct attggggcaa acgaaaagct gtggttattc agttttcgcg
     9661 tggttgtcct tacccgtgta catactgcgg acaaagtctg ttttggaaaa aatggcgaca
     9721 ccgtgatcca cagaaattag ctgatgaaat agaaatgctt caccgtgaat atggcattga
     9781 agtaattaat tttgcggatg aaaatccatc ttccaatcca aaagcctggc gagccttttt
     9841 agaagcactt gttgcgaaga atttaaattt gattttagtt ggatctattc gtgctgataa
     9901 tatagtacga gatgcggaat tcctacatct atacaaacaa gctggctttg agcgcttcct
     9961 tttaggaatt gagaattacg atgaagtagt ccttgaaaaa attaaaaaag caggcactat
    10021 ttccaaagac aaagaagcca ttcaattatt gcgtaaacac ggaatattat ccatggctac
    10081 ttatgtggtt ggatttggag aagaaaaaac acgtgatttt tacaatagtt taaaacaact
    10141 attatcctac gatccagatc aggtgcagct tttatatgtg acaccgcata aatggacgcc
    10201 ttattttgaa gaggtaaaag acaaagaaat catccttact gatcaacgaa aatgggatta
    10261 taaacatcaa gttatttcga ctcgatattt agcaccttgg ttggtaatta tttatgtaaa
    10321 gcttatcgaa atcatcatgc aaacacgacc aattgcaatt tatcgattgc tatttcacaa
    10381 agaagcacga ttgcgaagtg caatgcggtg gtatacccga attggtcgta gagtgtggtt
    10441 ttgggaatta taccagttct ttttcgtgac gcgtttatcg aaagaaaaaa tgaaaatggg
    10501 tgagttttgg aaatgaattc aactactgta taattttaac ccaattaatt ttaagctaca
    10561 tgaattctgt ttactcatca aataaactgt attaaaggaa cgcatatatt aataaattat
    10621 cccctaaata ttattttagt gcaaaattag catgttaaat aaaaccttca tcttgtatta
    10681 aaaatactat ttaaatcact tgaacagatg aaataatatt tagtaattta ggtaaactat
    10741 ttctttcaca tgaattcaac tttaaaacta attcattggg cacccagaat catttcgata
    10801 ttagcaatat gttttattgg aatgtttgca actgatgcat ttaattcaca cgaatctttt
    10861 acaaatcaat taaaagattt ttttatccat ttaattccaa catttatttt attggccatc
    10921 ctacttttag catggaaaaa agagcttgtt ggaggaatta tctttgctac tctaggactt
    10981 ggtttagctc cattcattta ccagcacaat tacactctta atcattcaat acttactagt
    11041 ttatcagtga tactagtaat aaatggacca tttattttaa ctggctttct ttttatctta
    11101 agtcatttca ctaagaaaaa aaacaccgct ttttattcca attaaagtat tctatcagaa
    11161 atactaatct gactaattaa gaatcaaaaa ataaaattca aacatatgaa agctgccatt
    11221 cataaagtat acggtccgcc cgaagttgtt accattcaag atgtacctat accagaaata
    11281 ggtgaagatg aagtattaat tcgtgttcat tattcaactg taaatcgtac tgattgtggt
    11341 tttcgaagtg ctgaatattt tatatcccga ttttttagtg gccttttaaa acccaagcaa
    11401 caaatattag gaaatgaatt ctccggacaa atagtctcta ttggctctct tgtaacaaag
    11461 tttaaaatag gcgatcgagt atttggatat aacgatcaaa agtttggtac acatgctgaa
    11521 tttatgagta tgagcgctaa tagtacacta gcattaattc cagaatcaat atcattcgct
    11581 gaagcagcac caactttgga aggaggacat tatgcttggt gtgatattct ggctgcaaaa
    11641 gtaaaacccg gacaacaggt tttagtttat ggtgcatcag gagcaattgg ctctgctgct
    11701 gttcaactac ttagttattt gaatgctaag gttatagctg tttgtggtgc tgagaacata
    11761 gaaatagtga aaaaattagg tgcgattcaa gtgatcgatt ataaaacaac cgactataca
    11821 acacttggag tccaatttga ttttgttttc gacgctgttg gcaaaacttc ctttgggaaa
    11881 tgtaaaccag tacttactaa agacggagtt tatatatcca ccgaattagg taaaaacggt
    11941 caaaatattt ggttggcgct tttatctaaa ataaaaaaag ggaaacgtgt actcttccca
    12001 cttcctacca ttaataatca agatgttgaa ttctttagag aattgattga gacaggaaag
    12061 ttcaagccag ttattgatcg aactttttca ctggatgaaa ttgtgaatgc ctataaatat
    12121 gtagaaactg gacagaaagt tggaaatgtt ttaattaaag tctttgatta aaattccaat
    12181 tttgacggat catatcttta tttgcgtgtt agatttttgt agtccttcat gaattttctt
    12241 aacaaaaatt aaaacaagac tcatatattt atcatcctaa acccaactct taatttcgat
    12301 atgaacccta tcactgttca agtacaaata aacagttcta ttcaaaaagt atggcaattc
    12361 tggactgatc ctgaaaaagt catgctttgg aattttgctt cggatgattg gtgttgtccg
    12421 gcggcaaaaa atgacttacg tgaaggtgga gaatttaact tcacaatggc agcaaaggat
    12481 ggttccatgc agttcgactt ctgcggaaca tacacatcgg taaatacact tcatcatatt
    12541 tcctatattt taggtgatgg acgaactgtt caaattgact ttaaagaaaa agatggcttg
    12601 gttgaactaa ccgaaacatt tgacccagaa tccgtaaact cattagaatt acaaagatcg
    12661 gggtggcaag ctattttgaa taatttcaag gttcaagtcg aaaaagccta atacaaataa
    12721 ttctattttt taaaatctgc tttcagaggt ctactttgat agcagatttt tttttgaaat
    12781 taagccttca gcaaattata cttctgctga atgggaatat tttagttctc agtgggtaaa
    12841 atgtgggacc caaaatactg gcattcgata gaactttgac tagttcatta ttcaagaaat
    12901 ctacccgata ttcttataaa tgaacaactt gttcaaatct aaaatcgaat cgcatgagaa
    12961 attcacttac tctattttta ttgatgtgct taattgttac aacacaaaca gcatgtaaac
    13021 gagatgataa ttcttccaac gatcaatccg tcccttcaca agtattaaat aatgtacaac
    13081 aagggaattg gataattact ttgtttaatg attcgggatc agacgaattg tatcacttta
    13141 caggaaagac ttttgcattc agcaatggca ctgtaactac gactcataat ggcgcaacta
    13201 tatctggtac atattctagc ggtacagatg atagcatgaa taaattcctg ttgaatttcg
    13261 gatcagtttc accttttgat gaactaaacg atgattggca tatcatagaa caaactaaaa
    13321 ctaaattaag actgcaagat gtaagtggag gaaatggagg aacagatcta ctcatatttg
    13381 aaaaagcaaa ctaagcataa gcactaacat tcaaccaaaa actcgcacac ccaaacccag
    13441 cctaaaaact gggtttcttt atatcctata cttccaataa aaaaaccaaa atcaaaagac
    13501 ttagtgctca tgattctggt attctgatcg ggttaggaaa attgcttttt ttaatccgta
    13561 taaaatttat aaccaacagt tccaagaaaa caaacgtttt tataacgagg cgttaatgat
    13621 gaatcatcgg ttgtgttacg aaagaaatcc cagcttgctc gcgcacccaa aaccatgtgt
    13681 ttcaagttga tatctgcacc acaaataaaa ccagcattgc tccttcttaa tgtttcattt
    13741 ctaaaaactt gctcttgcga ataactagaa aatgaagtag taaattcatt tcgctgttta
    13801 attaaatacg agtattggaa accagttaat aatgagatga attctgttgg tttgaaaacc
    13861 atctgcaagg gaatatccat aatgaatgat gttcgagtaa aattgtaaga actcgctaat
    13921 aattcacctg atcctttaaa acccttttgt gaaaccgata tttcaggctg aaaaccaaaa
    13981 tatttggtaa ttggaaaatg taccatagca cctgccgtta aagcatattt cgattcagca
    14041 tgaaattgag atccctgtcc agcaaataca tctgcaatac acattcccgc ctttaaacca
    14101 aactgatact tttcccgatt atctgtgggt aaattattag ttgttgtgct tgaattggta
    14161 ttaatggtca cgtttgtttt agtgctatcc tgcgcagaaa caattgaaaa tgagcaacaa
    14221 atccccaaga ataataaaat ctttttcatg atatctgtac taaatgaatt aagcttcgaa
    14281 atctgattaa tcaaaaaatt aactgatctg cttaacgtta ggacaaaatt gagaagacta
    14341 tagaatctat ttgttacata aaaaactgaa tcacttacat aattctctta gttgaattat
    14401 ttaagtagtt gcttttgcaa tttcattttc ttctcaccga ttaatgaatg aataattcca
    14461 ctcattatcg attggcttgc gtaatgtaaa aaatatcgac tggtgtcacg tttagcataa
    14521 attggactaa ttcgtaattg attagaatta cttgggtttt cagctatagc aataaaatta
    14581 ttaagtaata aggaacgcaa tcgaagatgc atcaacgatt ctttgggatt tacttttaaa
    14641 ttctcaattg ataaattgtg atattgaaaa ttcattttac cattggagta tttttcatct
    14701 gccttaaacc aaaaagagac agaatcaatg attccagctt ttatccggat gccttttgaa
    14761 ttttctaaca aagtattaaa tgaaacaaag ggcatcttgc caagtgagcc tttacaataa
    14821 aatacatttt gacttgaatc gaacggaaat tcgaattgaa ttttcaattt accttgattc
    14881 attaccaatc cactcatatc aatcttgaat ggctgtaata cttgctgctg atttattgta
    14941 taaatcccta gtgccctcac attcatttga gtaagaaaga tagaaccagg taaacgactt
    15001 ttaggtgcga gtacacgaaa ccgcacaaaa atagaatctg cttgaattgt atccactcga
    15061 agtttaaaag gtatttgacg tatcaaatcc tgcattgaag cgcaatatcg aatggctcca
    15121 gcaaactgat tattccgata tgcatctatt ttagcgtttc taattttcag tgttttaatt
    15181 cgtaattctt gttcttggaa aagtgtattt aaactaaaac cattgactat ccattcaaag
    15241 cttcgtatat ccatacgggt aatttgcatt ctagctgaat cggcaaaatc ttttgtatta
    15301 tatagtggaa ttaaatggat tgcttcaatt gataatgttg aatcgattcc ctcatatgct
    15361 aagttatttc caacaatacg atataattta ttcacttgag gaaagctaaa acttgggatt
    15421 ttcaatgttg ttggtccaac tgatacatcc aattgattat tcaagcctaa ttgttccaat
    15481 ttaaaatcat gtatggctaa agttgatcct tgtgaaatca gaatgagtcg tggattttgt
    15541 ttgttctcca tccaataaaa tgggaaggaa gaaaatgaaa ttcccccaac ctcaacagaa
    15601 taaggaattg atagcttttc atcgggcttt ttaattggat tatgttcaat cgaatcagcc
    15661 ttaaaataga caatcaatgg atggttaaaa ttaatctcat tgattttaat tttatcgtga
    15721 aatgttagat cccataaatc aatatgagct aattcaatac ttgagaatcg agaataaatt
    15781 aaatcacctt tagttttcaa tccttgtttt gggaagcatt caacatgatt caggaaaata
    15841 gaacgactta aataattgat tttaatttca gctaaattta attcgtattt gccagcacta
    15901 cttttccaaa ctaagagaga taacttattt cgaatgctgt tgattatcaa taaatgaatc
    15961 attaatcctg ttaaaatcag aattactcca acaacaaaca gcttcgttcg atggcaaatg
    16021 tattttttca gccattcctt cactaaccga aattaatgaa tcatttcatt taatctgtta
    16081 tagttatact atcattttgt acagaattca cacattttca aatacttgtt taaataaaaa
    16141 aacgccccat cgaatgaacc atggagcgtt ttattattta attaaatatc agtttaaaac
    16201 aaccaatgtt tgtttttctg aaccagcaag aacgatataa catcctgcct gaattccagc
    16261 tgtttgaagt ataaaaccat tggcagtttt ctccattggg atatttacca gtttacccat
    16321 actatcaaat acacgaatag tttgatcagc cttttctaaa tccaaacgaa tcaaaccttt
    16381 acccggattg ggatacatgt tcattaccgg ttttgttgta gctatttcta tcccagctat
    16441 tgttccataa atctctgaag aagcatcgaa acctcctgtt accaatgcat ttccatccga
    16501 cataactaca gctgcatgtt cataacgaga atcatgattc atggaagcgg ctacccacat
    16561 atccatggta ctatgatata aataagtaga ggaagcaaat gtggtaaatc cgctgccagt
    16621 tgagtagtta gaactatcta cacctgcaat taccaataca tttccgtcgc ccaaggtaac
    16681 ttgttgatgg taacttaaac caactggtag tgctgatgct gctgaccaag aatttgtggt
    16741 aggatcgtac aattcagtgt cggttaaacc attataggcg aagttgatag atccattaaa
    16801 ataacgtcct cctgttacta atacttttcc gtttgctaaa attgaagcag catgaaaatc
    16861 cctaccaata ctcatgttag gaccaggtga ccaagtatca tttgttggat caaataattc
    16921 agtggaattc aattgaaatc ctgcatttgg gttaaatcct ccggcaatta atacttgccc
    16981 cgaactcaac aaagtagcag tatgataaga acgtcctgtt gttaatgaag caatggttgt
    17041 ccattcttct gttgtaggat cataaagttc acaagaagaa agattattcg ttccatcaaa
    17101 acctcctacc actaatactt ttccattttg caatttggtt gctgtaaaat aagaacgacc
    17161 ttgagttaat tcgccagttg cagcccacat attcatagtt ggatcatata gatcacacgc
    17221 ttcggtatta aatgctcctg tgaaaccagc aattacaagt actttacccg aattcaacgt
    17281 tacagcagca ccattgtaat gctcactagc catatcaggt gttgaagtcc aagaatttga
    17341 acttgcatca taatattcgg cagatttgag gtttgttgtg taatcccaac ctccagctgc
    17401 taatacatgt ccttctccat aagcagtcat ggcatgacct gctcttgaag ttgacatcga
    17461 tgctacagta ttccattgag caagtgctag cccaggtgta atgatgcatg tggcaataaa
    17521 aagtaattgt ttattcatgt agctatgtgt taagaaatga attggaaagg gtttgattta
    17581 gtactgattg gacaaataaa gtaaaaataa ataatcagaa tgaccgtttc aggtgattga
    17641 atttgaacaa tgttattcaa tggatttgca ttcttaaaga aatgatttga ttcttcgaag
    17701 attaagatag ctttaccaaa taaataattc acattcagat tatcaaagag aaattgatga
    17761 aaacatatat ttcaattttg cgtggtatca acgtaagtgg acaaaaaatg ataaaaatgg
    17821 aatctctccg ggaattatac tcgggcatgg gatttcagcg catccaaaca tacattcaaa
    17881 gtggtaatgt agtttttcaa accgatgatg ctgatgttga ttatttgcaa gtaaacattc
    17941 aaaataatat ctctgaaatt tttggatttg atgttccagt aatggtattc gaatttgatc
    18001 agttcaagca aatgctcaat caaaatccct ttgtgaatga ttcggaaaaa gaagtcaatc
    18061 acttacatgt gacttttcta tcagccattc cagaaacaga gcgtgtggca aaaatggacg
    18121 aatttgatta tcaaaacgac cgttttcaac tcatcgataa agcagtattt ctctattgtc
    18181 cgaataatta cggaaatacg aaactctcca acaatttctt agaaactaaa ctgaaagtta
    18241 aagctaccac acgcaattgg aagacttctc aggaactcat tcgcattgca gaagctattt
    18301 cgatgcagga agattaattg ggattcttca ttgataaaaa ctattctaaa tttgccacga
    18361 atagtaaaaa acttatccat tcatgaaatt cctcaaacaa tcaccactaa aactcacatt
    18421 attctttgca cttaatttat ttttccttgt cgaaaacact caggctcaag ccttactaaa
    18481 ttcagagtgg agatcttctg gcacatttca aacggtactt caatccaact tttttgttga
    18541 ttcggtttac atgaaattcc tttcaaatga tgaatttcaa tttgtctatt ttgaaggatc
    18601 ggggcaaact atcgcaaaag ggctttggta ccaagatgat aattcacatt ttagatttgt
    18661 agatacttct gaaacatatc ttggacttcc ttgtcctcaa gacacattta gtatcgtata
    18721 ttctgttagt ggaaacacat tacaatttga tcaagtagaa aatggatgta caaccgtttc
    18781 ttcaattttg attggtagta gttggataaa cacagattta acagctggaa tcaatgtact
    18841 aaatttcaat aaagacaaag ccatctgctt tccaaatcct gcacaaggag gcatatttta
    18901 tcatttgaat caagattcta acctaacaga tggattaaca cttgaagttt acaattctgt
    18961 tggccaatcg attctaaaag atgtgaattc taacttaaat ggtaggatta atttggagaa
    19021 agttcctgtg ggactttact ttattcgatt cagtaatcat gctaaatcga taaattgggt
    19081 agaatctatt attctccaat aaacacttca aatacttttt taattaagta tttcagctat
    19141 taattccaat tgtatgttta gcaaattcaa gctattctgc aattggaatt atcgtttatc
    19201 attcatatta atggtggtag catcctgcac gtaaaatgag ctaattgccc cgagtatcat
    19261 aaatatgccc gctaagatga tggcataaat tgcttcggaa ttgtaaaaat gcttcacaat
    19321 tggaccacca aaaattccat ttacaatctg tggaatggtg atgaaaaagt taaatattcc
    19381 catataaacg cccattttcc cagctggaat tgatcccgct aaaatggcat acggcatagc
    19441 taaaatactg gcccatgcta aaccaactcc aatcatggag aaaatcaaga attctttatt
    19501 aggagcgaaa tacatagaaa tcaagcctaa tccaccacaa acaagtgaaa atgcgtgtgt
    19561 atttcttcgc ccaaaacgcg ctgcaataga gggtagtaaa agcgcatata atgctgaaac
    19621 cagattgtaa actccgaaaa taattcctac ccaagatccg gcatctctaa attgatcgga
    19681 agaagtgtct tcaattggta aatggtaaat atgctgtgca atagcaggtg tagtaaatac
    19741 ccacatgctg aacaatgcaa accagctaaa aaattgcacc aagcccaact gacgcatggt
    19801 tttgggcatt ttagcgaaat ctttaaatat ttgatttaaa cccgcttttt ctacttctga
    19861 ttctccatga atttcggcat actctgctgg tggatattct ttcgtgcgaa tgatcgtcac
    19921 caaaatggca aacaagaata ctgcagctcc aaagtagaag gaataaatca cgttatcagc
    19981 cacttgtcca gcaggagccg aagcagaaac atcaaaccaa ttctttaaaa tccagggtaa
    20041 ccaagaaccc accactgctc caactccaat gagaaaggtc tgtatagaaa atccaagtgt
    20101 acgttggtta tctggtaaat tatctgccac taatgcacgg aatggctcca ttgccacatt
    20161 aatactcgca tccatcaaca tcaaaaacat cgctccaata aacagcgcca ttttgccgac
    20221 gaaaaacaca cccgagttag gcaacaataa aagcgcacaa caacaaatta acgtaccgac
    20281 taaaaagtaa ggtttccttc tacctaatct gttccaagta cgatcgctat aatgtccaat
    20341 gataggttgt acaatcattc ctgtaaaggg cgccactagc caaaaccaag aaagttcttc
    20401 cacatgtgct ccaaaatttt gaaggattga agaagcattc ccattctgaa gtgcaaatcc
    20461 aaattgaatc cctaaaaagc caaaactcat attccaaatc tggcggaatg aaagttttct
    20521 gttctcagta cgtggtccca tgaaatgata ttagtgtcaa tttaacaccg caaaatacac
    20581 aattcgaaac atccttgaca tctaaggaga aaaatgaagg agcatatcaa tctgatgtgc
    20641 tgatgtgcta atgtgctggt gtgctaattc aggattctgc tgatgcttga atttgcaggt
    20701 agattagcaa tttgtcccct gagcttgtcg aagggcaaat cttcaaatca ttaaaaaaac
    20761 agatctaaaa accagaacgt taacttccaa cctaacgtaa tccccatttt caaattttcc
    20821 cgcctgtttg gcgggcaagc aaattttcaa atcattcaac cactctatca aacagaacga
    20881 actgctccat cctccgacaa accatcagca caccagcaca ttagcacacc agcacatcat
    20941 ttaaccatac caaactcccg cataacccaa taaggaaggt gaaaattcaa ttcttttgaa
    21001 aacaatccag ttttcccacc ttcatcaatt cgattacctg tatttattgg taaaacatgt
    21061 cccatacctg aaatctgata ggcaatcacc accgttcttc cttgtgaatt ggtgtaaaca
    21121 ctgcgttcca cattcggatc attatttatc aattctattt tatcgggaac tgcatccgat
    21181 ccatttgcag ctgtccactg agtaatttgc ttcgtttgat cctcaatagt aactaataca
    21241 tcattgcttc cctggacagt tagtagcaat ggatacgttc ctgtataacc tgggtaacac
    21301 gcgcgaagtt tctcgagttg agattgggga acatattcat cagtatcgta taaatctttc
    21361 atctgtgata aactcccttc ccaagttcca atggctggtc cagctaaaga acaacctgct
    21421 gtgatttctt ctggataagc tgccataaga atggttccca tgaccgctcc tgctgataat
    21481 ccaaatacaa aaacacgttt gttgtcgatt ggataatgcg ccatacagta ttgaatcatt
    21541 tgatgtaacg attctacttc acctccgcct cgagtaatat cttctttcct aaaccagtta
    21601 aagcaacgcg aaggattatt catcaaatgc tgctgtgggt aaacgaccaa cattcccaat
    21661 gaatcggcta atttgttcca tcccgattga ttagacactt cctcagccgt ttgactacat
    21721 ccatgcaaga ccattaacaa gggtgcatcg gaatttagaa gcgtcttagg tttatagata
    21781 taacaccgca attcacccgg attcgaacca aatttttcga cttgttcaat ttgggccgac
    21841 aaataattgg aaatcagcaa aaaacacacg accatcaata gattgaagaa aagaagtggg
    21901 tttcttgatt gctggttgaa tgtcatggag tgtcgatatg gaattcggac gaaatatagc
    21961 gtaaatcacc gccattgtta acaactcata cccaaacact aggtaaaaaa gctagcttgt
    22021 tttctatttt tgccacccat ggccgaagaa acaccagaag acttaaacga acagctcgaa
    22081 tcgcccgaaa acggtggttc agaggaacgt atcacgcatg tctcaggcat gtatgagaat
    22141 tggttcctcg attacgcttc ctatgttatt cttgagcgtg ctgttccata cattgaagac
    22201 ggtttaaaac ccgtgcagcg acgaatcctc catgcaatga aggagctcga cgatggtcgc
    22261 tacaacaaag tggctaacgt gattggtcat accatgaagt atcacccgca tggagacgct
    22321 tcaattgggg atgcattggt tcagttagga caaaaggatt tattgattga ttgtcaggga
    22381 aactggggaa atatttacac gggcgactcg gcagctgcac ctcgttatat cgaggctcgt
    22441 ttgtcgaaac tagctggtga aatccttttc aacgcgaaaa ccactcactg gcaattaagt
    22501 tacgatgggc gtaataaaga acccatcact ttacccgtaa aattcccttt actacttgct
    22561 caaggtgttg aaggtatcgc ggttggttta gcctgtaaaa ttctgcccca taactttatc
    22621 gagctaatcg atgcaagcat ttcggtgctc aagaaaaagc ctttcgaaat ctatcctgat
    22681 tttgtgacgg gtggaatggc cgattttacc aaatacaacg atggtgcgcg tggcggaaag
    22741 gttcgtattc gagctaaaat atcacagctc gataagaaca cactggtcat aaccgatctt
    22801 ccatttggaa caacaaccgg ttctttgatt gattcgatac ttgctgccaa cgacaaggaa
    22861 aaaatcaaaa tcaagaagat tgaggataat acttccgatg tagttgaaat tctagtccat
    22921 ctaccaccgg gaatttctcc ggataaaact atcgatgcac tttatgccat cacttcgtgt
    22981 gaaatatcgg tttcgccaaa tgcggtaatc attgaaaatg acaagcctcg ttttgtcggt
    23041 gtatcggaga ttttacgcct ttcggttgaa aacacggttg atttacttcg tcgagaatta
    23101 gagattgaga aatctgaact cgaggagcaa tggcagttca gttcgttgga aaaaatcttc
    23161 atcgaagaac gcatctaccg cgacattgag gaatgcgaaa catgggaagc ggtaattgaa
    23221 gccatcgaca aaggattaaa gcctttccgc aagaaacttc gtcgggatgt gacacatgag
    23281 gatattgttc gattgaccga aatccgaatc aaacgtatct ctaagttcga tgcttttaag
    23341 gcagatgagt acataaaagg attagaggat aaaatcgcga attgcgagca tcatctagct
    23401 aacctcaccg attatgcaat tgcttggtat cagaatctga agaagaaata cggaactgga
    23461 agagaacgca aaaccgaaat ccggatgttc gataccattg aggcttcgaa agtggtaatc
    23521 aacaacgaga agttgtatgt aaaccgagag gaaggctttg ctggatacgg aattaagaag
    23581 gatgaatacg tatgcgagtg ttccgacatc gacgatattg ttgtgatgcg tgccgatggt
    23641 aaaatgatca tctccaaaat cagcgataaa gcttttgttg gaaaggatat tttgcacgtg
    23701 aatgtattcc gtaaaaacga tgatcgaact acgtataatg tggtttatca ggacggaaaa
    23761 acgggtccat attacgtaaa acgctttgct attagcggtg ttattcgtga taaggaatac
    23821 gacatgacca aaggtaccaa aggcagtcgt gtggtttatt tgagtgcaaa cccgaatggg
    23881 gaagttgaaa cgcttcagat tttcctaaaa cctcgtccga agctgaaaaa gaccgtgttt
    23941 gagttcgact ttggcacctt ggcaattaaa ggacgtggtg ctggtgggaa tatcatgacc
    24001 aaaattccta tccgtaagat catcttaaaa gagaaaggtc aatcgacttt aagtgcaaga
    24061 caaatttggt gggatgatgc cgtgaaacgt ttaaatacag atggtcgtgg cgatttactc
    24121 ggttcgtttg ctgcaggtga taagattttg gctttgatgc aaagtggtac atataagttg
    24181 tatgaaccag atccaagtac acactttgat gaagacttga ttcaattagg cttttttgat
    24241 cagcgtcaac ctgtttctgc catttattat gatggtgaaa gcaaggaata catggtgaag
    24301 cgtttcttaa ttgagactac cgataaaaag acaaccttca tcagcgaaca ccctaattca
    24361 cgtttggaat ttgtctcaac cgatacattt ccgcgtgttc aacttgagtt cggtaaaatg
    24421 gggaaattcg aacggaatgc ccaggaaatc ctgcttgaag aatttattgc agtcaaaggt
    24481 ctaaaagcca aaggcaaacg tttatcgacg catgagccta aaaaaatcat tgcattggag
    24541 tctttgcctg aacccgaatt agaagaagtg gaagaaatag agcatgaaga ttccgattca
    24601 gaacaaacac caagtgataa acccaatgtg atcatcgacc tagaaggaga tgaaccacaa
    24661 atgcgtttat tcgaagagcc ctgatatttt ttactccttt taaaatcctg attgataaac
    24721 aaatgcacgt ttatcaacag gatttttttt atttattttc attctattct tatttagacc
    24781 aaatctaaat agactcattt tatatatttg ccccacctaa acgctaacac attattcttc
    24841 tcatgaaacg catatcaagt ttactcttgg cactttgctc attaggagcc agtgcacaaa
    24901 ttatcacccg tgaggattca ttacatgcag gtttaactcc tagcaccaat ccaacagtta
    24961 tttcgggcta tggacaattc aaagccgaat acgatactcg ctatcaaaca gcaaaagcaa
    25021 gtcttactcg aaacgtactt ttcttcggac ataagttcaa taagcgtatc gcctttttct
    25081 cggaaatgga attagaagat gctaaagttg ttggaggaag tccctcagga gaaatcagca
    25141 tggaacagat gttcctgaaa tttggcatca ataacgacat ttacttaacg gctggattat
    25201 tcatcccacg tattggccta gtaaacgaaa accacttacc tactatcttc aacggaaatg
    25261 atcgtccatt cgttgaacgt cttcttattc cttccacttg gcgtgaactc ggtgttagtc
    25321 tttacggtcg tagccgcaga attcccgggt tgaactattc cgctggaatc atgaatggac
    25381 ttaattctca aggttttggt aatggtgaag gtattagaga aggtcgattt gaaggaaaca
    25441 atgctacagc aggcaatcta gctgtaactg gagcactact atactaccgt caaggatttc
    25501 gttttcaagc ctctgcttat tacggaggaa gtgctggatt agtaaagcgt gtagcagata
    25561 gtctacaatt agattcaaga ccatttggaa ctcctgtttt ccttactgaa gctaatgttc
    25621 aatatcaaag tcagagtggt ttcgaattta aagttctagg aacgctcagt acgattaaag
    25681 atgctgcttc gatcaatcgt gcatactcca ataatactcc tggaaaaatg tggggcgctt
    25741 atgctgaaat tggatacaac atcctcaaag cttttaaacc gaacaccgaa cgtagcctag
    25801 ttctatttgc ccgtgcagaa gcaatggatt tgaatgcaga agttccattt aatggcattc
    25861 ataatccgga gcaaaacaaa caattcttta tcactggaat cacttacaca cctgttcgtg
    25921 gagtagtagt taaagtggat tatgtacatc gaatgactgg cacacccaat ccagctttga
    25981 tcctcactcc atacccttct gctttaccgt atttcaacag taatggcttc ctaaatattg
    26041 gagctggata ttctttctaa acatgaaccg gagaaatttc atcggaaaaa ctgctcgact
    26101 aggtgcctgt tccttaattg gagtatcact actaacgcac agcggatgtg cctcatatcc
    26161 acttgttaag gtaaagccag ttaacaattt acttaaagtt gatttaacag cttttggtga
    26221 aggtaaacgc cttcttgtta gaacaccaga tttagagttt gatgtatttg ttcatcaatt
    26281 tcaaaccgga caattccgag caactcttct ccaatgttcg catcgtaacg agcctatttc
    26341 tgtttccgat acgcagattt attgtccaag tcatggaagc acattttcac tcgaaggtgc
    26401 tgttactaaa gctccagcta ctgaaccctt gaaacattat ccagtcaaat tatcggatga
    26461 tggaacttct ctcttaattg atatttcctg atttgttcac ccaacccaac ccgaacatgt
    26521 tgttacctaa taaattcctc ctcacctcat taactgtact cgcactcgtt gcttgcaata
    26581 aaaaagacga tacgcccgaa ctttccgaca catttgcttc tcgaaaagtg attctttcgg
    26641 atatcagtca gcacgttatt gctgccactt atgctgatat gcaaacccaa gccaatacgc
    26701 ttaatactga aattaccgag cttcaaacct caacaacaga tgctaatcta actgaatgtc
    26761 gaaatagctg gagaaatgtt cgttcagcct gggaacaatc cgaaggtttc ctttttggtc
    26821 cggtagcttc tgaaaatatc gacccaagaa ttgatacttg gcctgttaat cacattgatc
    26881 tagatagcat tttagcttct tcggttgaat ttactccaca atacatcgac gaacttcaag
    26941 atgctttgaa aggcttccat cctgttgaat acttgttatt tggattgaat ggcgataaaa
    27001 cagcagctca atttaccgac cgcgaactta gcttccttaa agcccttgct gacaatttac
    27061 aaatcctttg ctcgcaagtt tctggtcgtt ggaattcatc aagctctgat agctacttaa
    27121 gtaaattcgt taatgctggt tctggaagcg acgaataccc cacccagctt gcagctttcg
    27181 aggaagttgt caatgctatg attggcattt gtgaagaagt tgctggaggt aaaatctccg
    27241 aaccttttgc tgcgcaagat cctagtttgg aagaatctcc ttatgcacaa aactcgctta
    27301 aggacttcat ttccaatatg aagagtgtga aaaacattta ctatggctcc tacaaaacaa
    27361 gtggtcaggg aattaaagac tacgtgcgtt tgtataacct ttctcttgat gctagaatca
    27421 aacaagacat tgatcaagca attgctgcat tagaagctat ttctgatcca ttcggactag
    27481 caattagtac acaaggaact ttagttcagc aagctatcac tcgtattgaa acactcaaag
    27541 aaacgctcga aacacaattg ttaccgctta ttcagcaaca catcaaagaa taatcatgat
    27601 tcgcgttaaa gttcattcag ttcctttctt taccctgttg ctacttggct cttttgcttc
    27661 gtgtaccagc aaagaagatt tccctgaaac atcacacaat gagtattatg ccggtggaaa
    27721 tcaaaccgct tttatttctg gtgcaggtgc ttttagttct gcatttcctg tcttaaatgc
    27781 tagccaaagc cgtgttcatg aaattggcga tattggattt gaagcaactt tcgtgagcgg
    27841 ttcgcaagta gatttaggtg gacttggtcc ggtttataac aacgtttctt gtggaagctg
    27901 ccacattgcc gatggtcgtg gaaaagcacc tgaggctggc gaatcgctgg cttctttgtt
    27961 aattcgtata agcgttcccg gaactaacga acatggcggt ccacttggtg ttccaggtta
    28021 tggcggacaa tttcaaccac gctctattta cggtgtggcc aaagaaggtg atgtgaacta
    28081 ttcctatgaa tatttacatg gatcatttgc tgatggcgaa acgtatgaat tacgccaacc
    28141 tgttatagaa ctgaataata cctactctac tttaccttca gatgtgatgc tatctccccg
    28201 acttgcctca ccagtattcg gacttggttt gttagaagct attcctgaaa gtagcatttt
    28261 agcacatgaa gatgcgcttg atgctaacaa cgacggtatt tcgggtaaag ctaaccgtgt
    28321 ttgggaagtg caaaaaaatc gcttctcatt aggacgattt ggttggaaag ctggacaacc
    28381 gagtgtaatc caacaaagcg caggtgctta tgtagaagac atgggcatta ccaataccat
    28441 tttcacgttg gaaagtgtgt atggacaacc tcaacaaaat gccggaaata cacacgttga
    28501 gctcagtgat agtactttac atgctgttgc attttatgtt caaacattag ctgttccagc
    28561 tcgtcgtgat gtagaaaaac cgcaagtaca acttggtaaa accctattta cacaaattgg
    28621 atgtgccaat tgccatacac ctcaacaaag aacaggtgtc gatgtagttt tcagagctgt
    28681 ttccaatcag gttatttttc cttataccga tttactttta cacgatatgg gggaagaact
    28741 tgccgataat cgtcccgaat tcgatgccga tggaaaagaa tggcgtactg caccactttg
    28801 gggaattggc ctaacgggag ttgtaaatgg gcacacaaat ttcctacacg acggacgagc
    28861 gcgtaacttt accgaagcca ttctttggca cggtggagaa ggcaacaact cgcgccaagc
    28921 atttaagaat ctcgcgtcaa acgaccgaca agcattgctc gcttttctta agtcacttta
    28981 acccaacccg agcaattaaa agccggaatt agcaatgatt ccggctattt ttttaggtaa
    29041 ttcccaggtt ttccatgctt gttcccatgg agcttatcgt atgccgaagc cttggcgaag
    29101 gcttaaatcc ctaagctcac gtttagttca aacatttagc tcttcataat cttatagcgg
    29161 aaacctgcat aaaccattcg cccaaaaccc ggtccccagc tataagaagc atcaaagtat
    29221 ttcgaatgag gatcatcact cgaaataatc tgattttgct gatacacatc taacaaattc
    29281 tcaacgccta cataaacagc ccaacgattc aaattacgtg tcacctgagc atgaaccatc
    29341 acataatcgg gtgaatgtga atcagcttga taagcaaccg gatttaaaac acttgaaggc
    29401 aaacgcttac ttccaatgaa attggctgta gcatcaacta accatttatc ttttcgagaa
    29461 tgaaaagcca aattcaacaa agctctatgt ggcgatacaa atggtcgttg aaatgttcca
    29521 ctcaaatact gagtttgggt ttgagtaaac ttatacgaga gacgcaaatc gaaacgcttt
    29581 ccaagtgctt ggttataatc tacttgtgtg cttactgact gagaattccc atccaaatta
    29641 tagatacgca attgttgtgg cgagaaatca gcatccacca caacttgtcg ctcaaaccaa
    29701 gtcatataag catctgccga taaacttccc tcctgtccgg ctagttcaaa ctgcgtacta
    29761 aatcctattc ctcctgtcca tccaatttcg ggaagtaaac catagccttt cccttgcgct
    29821 gcagcatttg ctttaactag caacaaacgt gaactcacca aatacgagat attctcatcc
    29881 aaaattgtcg ctgttcgctg tcctcgccca cccgacacat gcagagcagt cttctcactg
    29941 aaagctaact tataatgcaa acgcggtgtg gcgaagtttc catataagaa attatgatct
    30001 gcacgaattc cagctaccaa agtcgacatg cccgatttcc aggtgtattc gaaaaatgcg
    30061 cctgcgacta gttctttacg accaaatggt tgacccgtaa aatactcttt catcgaatca
    30121 tattgaacat tgaaacccgt tcggtaggta tgattggaat ttccgataaa gctttgaaaa
    30181 ataaggctac cataagcact ctgctgatct gcatcgtacc aacgattacc caaaattgtc
    30241 ctttgctgat tcatctgagc attccccaaa ataccaatac tcgttaggga tcttccctga
    30301 aaaatgtatc ccagctttcc aaaagcatcg aaacgaagca catccttgtt aaagcgccaa
    30361 tcgtgtaata catcaaactc gtttccttga tgtgctgtac ctgctctccg attatcaccc
    30421 aaagcctgta ttccgaattg ccccgataac tgcttcccac tgcgatacat ccatctatta
    30481 agtatgttcc actgattgcc gtccgtcaca tccataaact tatccttgtt tcgatcaatg
    30541 ggttttttca tttgatctcc atgaaccaac aacacagttc cccatttttc attcaaggca
    30601 attgctttac tcagattcac ttccattcgc cccatttgat tcacataccc attcaacaga
    30661 agtgtttcat cggtaaacgg tttcttcaat tccagattta tttgtccggt aatcgattca
    30721 aacccatttg ctaccgaacc aacacctttg ctaatctgta tcgattcaat ccaagttccg
    30781 ggcaattgtt gcaaccccga agaactcgcc aatcctcgca tagcgggtaa attctcctga
    30841 gttatttggg tataaattcc cgacaagccc aacatggtaa tcttcttcgt tccagtaacg
    30901 gcatcggtcg tacttacatc aatcgaatta ttcgtctcaa aactctccga taaattgcaa
    30961 caggcagcct tacacaactc ggcagttgtt atgacttctg ttttaattgc accaagctta
    31021 gataaatagg ttgatgattg tttaccagct atttccagcg ttcttaattg agtgcgttga
    31081 tgtagggtta ctcgaacaaa ttcagatccc gtaagtgtaa gtgtatctgg tctgtatcca
    31141 gtaaatgaaa gtatcagttt cgaatgtaga tgctcatcag caatttggaa atttcctaaa
    31201 ctgtcgcttt gtaccgctat gtttgttcct aaccaataaa ccgacacgcc agttaaagga
    31261 agagtaattc cttcatcatt ttcttctaat acttgaccgt taattgtttg tgacgttcct
    31321 tccaatgcac aaagcatgag gaacaacatg agctgagttt tcatgaatct ttctgaatga
    31381 agtgaatact atattgattc agtaagagtc taacattttg ggctccggta tacttggttc
    31441 cattcgctca taatgacagc aattatgcaa tttctcatag gcggcgctat cagctttgag
    31501 ccattcagaa tcgtgtcctg caagtgaaat ttcttcaata attttatcga gcgtgatttt
    31561 cttgggatcg tagttaacgg tcaatacttt cgttttcgcg tcccagctag cttttttcac
    31621 tcctttcaaa tcgacagccg attcgatacg ctttttacac atatcacaat taccagctac
    31681 aagaatggta gaagtagttt gagcaaatac aggaaatata aataaggcaa aaatgcctga
    31741 aagaaaaaga gatttcatgg tttttaggtt tgagttaatg aaagaaaaca atgtttcaat
    31801 cagaagctca aatcctccat tgaccatatc caactaatgt atctggtggt ggtaaatcaa
    31861 ctttacggtt gtctaccaac tgaactttat cactagttga agttatattt attaacgaat
    31921 agcattccga tagaagtgta aaagcttgta aattaggtat gcttacactg gttgaattgg
    31981 cataactgtc tttaacctgc aataggaatt gcgcatcggt acaacaattt gattcttcta
    32041 cttccgattc acaagcacag cttaactgat gaaaaggatt taagctaacc gaactaaatt
    32101 ctccgtgaca ataatgtgct tgcatacgaa ttcccaccgt actaaataag tacagaaata
    32161 aaatgggaaa aaggagcaaa cgatttaaca tactatactt gaacttgaag cggttcggcc
    32221 gaataaccta ttgatttcaa tgcatcacat atctgccctg catccatatt ttcgagataa
    32281 acactcaata tttttcgtgc atcttctgtg ttcactttcc aattgtttgc tcctactaat
    32341 gcatccaaag tcggagtcac tgctctaaca caacttccac agttaatact agtactaaac
    32401 tcaagtgttt tcatctctta cacaacgatc taagtgtagc tttgttgtaa atggttgcct
    32461 atccaaacga attcgagtag gtttgacctt tatgcttatg aaacacaaaa atagaataag
    32521 taaagccagt atattccttt tgctggtttt aattcagctc ccaactttca gtttttctca
    32581 aaatgcccga aaattcaggg ttttctttgc tgataaaccg gaacagaatt ttgatccata
    32641 ctcctacttc gatccggcaa caattcaacg tcgaaagcaa caaaatctac ctatttatga
    32701 ttggtacgat ttgcctgttg cccaatccta tctgaatcaa atcggacaaa actgtgaacg
    32761 tatcaaaatg cactcacgtt ggctcaatgc agctgttatt atggcgaatg atgagcaaat
    32821 cgagaagatt aggtcattct cctatgtaaa aacgatcgaa gaagccgatc caatgcagat
    32881 tcagcttgct tctattgatg aggtagaaca aaatcttcaa caatccgatt caaatgaaga
    32941 tgaagtccta aatacccaaa tcaaacgcat gggtgctgat cttcttgctg aaaagggatt
    33001 aaccggtaaa ggaattcgaa tcgccatttt tgatgtagga ttttcgggtg ccgacaaaca
    33061 cactgcattt caacatattc ggagtcgaaa cggagtgttt aaaacttggg actttgtcaa
    33121 aaatgaagaa aatgtttgga aaggtggatg gcatggaacg gctgtttggt cgtgtatagc
    33181 tggaaaacta aaggggcgct ggtcgggctt agcaactgat gctgattttc tccttgctcg
    33241 taccgaaatt ggtagtaaag aaccatttgc tgaagaagag tattgggttg ctgccgccga
    33301 atgggctgat aaaaatggtg cagatatcat caatagctca ttaggctata ccgattcacg
    33361 ttatttccct cgtcaaatgg atggaaaaac gaccttcatt acacgaggtg ctaatcttgc
    33421 agctaaaaaa ggattgttag tagttaatgc agctggaaac gaaggggata gcaactggag
    33481 aattattgct gctcctgccg atgccgattc tgttttaacc atcggtggcg tggaaccttg
    33541 ttgcgattat cacatctcat ttagttccta tggaccaaca cgcgatatgc gtctaaaacc
    33601 taatgtttgc gcccaaggcc gtgcattttg tgcgaaagaa aatggagaaa gtaatgccga
    33661 tggaacttct ttcgcgagtc cgcttgctgc tggtttcgct gcatgtgcat ggcaagccaa
    33721 acgttcgctc agtaacatgc aactatttga cgaactgcaa aaatgcgcta gtctctaccc
    33781 ctattttgat tatgctcatg gctatggtgt tctttcggca gatcgttttc tcaatagtat
    33841 agttgcaacg ccaacctttg ttgctaaaaa agctaatata actagtgaaa cagaagattc
    33901 tggcgagtac atccaaattg aagtggatga taagtacaca accgtaaatc cagaaggaat
    33961 ggattatctt ctttactata gtatcgttaa tacctcgggt ggattagatt cttacacagt
    34021 tataaaagtt cacgaaaaaa tccctgttca gttcaagact aaagcctatt caactggaac
    34081 taaacttcgt atctctttcc aaggctatat gtctgaaatt caattccaat aatgaaaaaa
    34141 ctcctttttt cttttgctgt tattattgcc ggcatgaatc aattagctgc acaagatgta
    34201 gtttttaaaa tggatcctgc agttgatacg accgaatcta aatccggtcc gaacctcaaa
    34261 aactactggc atccgtttat gagttatgga ttctgtattg atccaggaag cgcaggtgcg
    34321 accgtaaagc aacctcatct tgatcagttt agctttggtt atcgttacaa aaatcgttta
    34381 gctgaacact tagcagttgg tttggatatc tcgtataacg taaacgatta ttcgctcaaa
    34441 caaaatgcat ccaaaacgct tcccgatact gttcaatata aaaagcaacg gatgatattt
    34501 tataatgccc aggttggagc gtatgtgcgt attaactttg ggaaaagagg taatacatta
    34561 ggaaattatc tcgacctagg tggatatggt gattatgtga tgtcgcacgt tcagttcact
    34621 aaattcaact tgcctgatgg ttccatcgtt cgagctcgta gaactcaact ggattattat
    34681 caaaaaatca attacggttt gctcgcccgc attggattta agaaacttgt catttatggt
    34741 caatatcgta tttccgatat gttttatcca tcattaaaaa tggctgagct gcctcgaatt
    34801 caagcaggta ttcaattcgt tcttcgctaa ctaaggtgcg catagctgtt aatactcgtt
    34861 tgttgcagtc gggaaaactc gaaggaatcg gttggtttac tttcgaaacg ctccgacgca
    34921 tcacacttgc gcatcccgaa cacgagtttc tgtttctatt cgaccgtcca tttagtgagg
    34981 agtttgtctt tgcacccaat gttcgagcgg tttatgctgg tccgcctact cgtcatattt
    35041 tccttttctt tccttggttt gaatggcaga ttccacgcct gctgaaaaaa catcaagtcg
    35101 atttattttt aagccccgat ggtactttat cacttcgtag tgatgtccct cagattgctg
    35161 ttatacacga tttgaatttt catcatcatc ccgaacaact gccacgcata gttcgttggt
    35221 attacaatac tttttttcct cgttatgctg caaaagcagc tcgaattgct accgtttcgg
    35281 aatacaccaa acaagacatc atcaaaagct acggtatagc tccaaataaa attgatgtta
    35341 cttggaatgg ctacaacaca cgttataagc ctttaggaga tgaaattaat gaccaaactc
    35401 gagcacaata cacgaacggc aaatcctatt tcctttttgt aggattaatc attcctagaa
    35461 aaaatctgat tcgtttaatg ctggcatttg atcgttttaa agaaaaatcg aaatcggatg
    35521 ttcaattact tgtagttgga gaaaaaaaat ggtgggatca agaacatgaa aatgctttcc
    35581 aaaatctcca gtttaaaaac gaaattcatt ttgccggacg actacaaccg gaagaattac
    35641 ataaagtact ttctgctgct caagctttaa cgtttgtccc cacatttgaa ggattcggaa
    35701 ttcccatttt agaagctttc tctgctggaa ttcctgtaat tacatctaat gttacttcta
    35761 tgcccgaagt tgcaggaaat gcagcattat tggccaatcc ttttgatgtg gatgatataa
    35821 cggaaaaaat gattgaatta aatcaatcgc ccgaattaca aaaagtgtta attgaaaaag
    35881 gcaataaaag aaaagaagaa ttttcttggg ataaaactgc ccaacttctt tggaattgta
    35941 ttcaaaaaga aatcgattat atcaatcgat cataattttc cttttggaca aaaaaaatcc
    36001 ggtcccgcaa gcgagaccgg caaaacaaaa aaattaacct a
//
LOCUS       DAOOQW010000006        30055 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_1775_length_30055_cov_5.313967, whole genome shotgun sequence.
ACCESSION   DAOOQW010000006 DAOOQW010000000
VERSION     DAOOQW010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 30055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30055
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1775_length_30055_cov_5.313967"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="PK281_00970"
     CDS             complement(<1..192)
                     /locus_tag="PK281_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013749778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HOY47937.1"
                     /translation="MQVIHISAECYPAAKAGGLGDVVGALPKYLSQAGHPALVIIPKY
                     KQKWILNQPFERIFSTLVRL"
     gene            complement(268..846)
                     /locus_tag="PK281_00975"
     CDS             complement(268..846)
                     /locus_tag="PK281_00975"
                     /inference="COORDINATES: protein motif:HMM:NF016270.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HOY47938.1"
                     /translation="MNPLSKFFRHIFRRFTSYFLKGLVLIVPLGITFYVVYKVFMFLD
                     GLIPYEVPGVSILIIIAGTTFLGIIGTALIQTPLKSLFNKTIDRVPLIKVIYTSVRDM
                     LAAFVGNEKRFNKPVMVKVGRDADLYKLGFVTQKDLTYLGIKEGKSAVYLPHSYNFSG
                     NLFIVDNDLITPIDAPSDEIMKFIVSGGITRI"
     gene            complement(891..1217)
                     /locus_tag="PK281_00980"
     CDS             complement(891..1217)
                     /locus_tag="PK281_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HOY47939.1"
                     /translation="MNWIILIIAGLFEVGFATCLGKAKESTGQTATWWMLGFFVCLTI
                     SMLLLYKATQTLPIGTAYAVWTGVGAVGTVLVGLIVFREPAEFWRIFFLGTLICSIIG
                     LKVVSH"
     gene            complement(1214..1558)
                     /locus_tag="PK281_00985"
     CDS             complement(1214..1558)
                     /locus_tag="PK281_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014033064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HOY47940.1"
                     /translation="MNIEEVREYCLNLPHVTEDFPFDEFTLVMRIGGKIFTLIPLEKG
                     GQMNLKCDPERAISLREEYSAIEAGYHMSKTNWNTLHFEHLPAELVKELIHYSYDLVY
                     AGLTRKVKESLK"
     gene            complement(1635..2117)
                     /locus_tag="PK281_00990"
     CDS             complement(1635..2117)
                     /locus_tag="PK281_00990"
                     /inference="COORDINATES: protein motif:HMM:NF013224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HOY47941.1"
                     /translation="MSSPADKLDAIDYKILKLLQEDGRMTNLELSNQIGLSPAPTLER
                     VKKLEKQKFIDGYHARLNRSKLKLGVSVFIEISLARQVENVINKFRDRIMGIPEIMEC
                     YQITGNSDYLLKVLVQDIPAFEKLIADQFSKMEEVRHVETMLIISEMKQSKTLPLKYD
                     "
     gene            complement(2173..3285)
                     /gene="hemW"
                     /locus_tag="PK281_00995"
     CDS             complement(2173..3285)
                     /gene="hemW"
                     /locus_tag="PK281_00995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HOY47942.1"
                     /translation="MLYIHIPFCKQACTYCDFHFTTSTHYKSHMVEALIQEMEFRKSE
                     FILPLKSVYFGGGTPSQLSNSELSRLMDYIHSNLGIATNAEVTLEANPDDLSREKLLQ
                     LKDQGINRLSIGVQSFFEEDLRFMNRAHSANEALNCIALATEIGFKEFSIDLIYGTPG
                     LSDERWISNLRQAASLPVNHLSCYALTVEPNTPLAKGIRKGNYPNPDEEQAARQFEIL
                     QSLAPELGFLHYEISNLARGEHVAVHNSAYWSGKSYVGIGPSAHSFNGKIRRENVANN
                     NRYIRDLAQGDCEHTLEVIDEKTRYNELVLTGLRTLNGIDKAALIKFGKEAVQYFSSA
                     RKQLPNPEHIIETETSFRLNPDAWLLADGIASRLFQ"
     gene            complement(3310..4779)
                     /locus_tag="PK281_01000"
     CDS             complement(3310..4779)
                     /locus_tag="PK281_01000"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOY47943.1"
                     /translation="MAVIIISVSLWYTNVLVKKVSTEERKKVELWADAVRNRAELVKY
                     TESLFDRLKTEERKRAEIWAEATQNLSITEDDDARSFYLKVVSENNTIPAIIVDQQNR
                     VNYITNADTTGFGKAEFFTGKVKAAFSAHRPIVIDDGFSNTFFIYYQDSRVFSELREV
                     LDNIIKSFISEVVVNSASVPVIVVDKKKTRVIASGNLDSTIIADTTAMLKLTTSMASE
                     NTPLEIELPDYGKCSIYYQGSFLLTQLKYYPVIQFTVIALFMILAYFLFNFSRRAEQN
                     QVWVGMSKETAHQLGTPLSSLIAWIEILRMKGIDEETLGEMGKDIKRLEIITERFSKI
                     GSKPELKTQDLQQVLEESISYMRPRTSRKINIDLVNLLPEDERMNVKLNVPLFDWVIE
                     NLFRNAIDAIETSGSLEVQFGDKGKYAYIDVIDTGKGIPKSKLKTVFQPGYTSKKRGW
                     GLGLSLAKRIIEDYHKGKIFVRRSEVGKGTTFRILLPKA"
     gene            complement(4829..5704)
                     /locus_tag="PK281_01005"
     CDS             complement(4829..5704)
                     /locus_tag="PK281_01005"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF020140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOY47944.1"
                     /translation="MSQARILWSTSGAGVRLIIINVIAFVLVFVPISILHLLNRDADG
                     VLILSILELSGDPLKLLFKPWTIITHLFVHRGLMHILFNMLTLFFTYQLFNRYFDDRR
                     FINVYFVSGFAGAFVFLLSVNLFPLFNVLPINYTAVGASAAVMGVLIAICTYRPDDEV
                     FLFGAFKMKLRWLALLFVLLDLISISNGNEGGHLAHLGGALFGFIWAMNLKKGRDLAS
                     FFGKIQDLLSFQKSRKSNLKVAHKRPMKDEDWNLNSKIRQKRIDEILDKISRSGYDSL
                     SKEEKALLFELSKDK"
     gene            complement(5701..6537)
                     /locus_tag="PK281_01010"
     CDS             complement(5701..6537)
                     /locus_tag="PK281_01010"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOY47945.1"
                     /translation="MNSGSPYSITPVVRTLIIICVALWLLSIGAGESGIDLSRYLGLF
                     NFDSPFFQPWQLVTHLFMHDTKEPFHLFFNMLGLWMFGSSLERFWGSKRFLIYFLITG
                     IGAGTVQTTVTNFQFRPMLEAANAYNMHPNYISFDRFTTNYLPGSYDNEQLNEFMKAW
                     YYDLKNPGYVVESKKLVAEVVEFRQNTVNVGASGSLYGLLLAFGMIFPNVMIMMLIPP
                     IPMKAKYFVMVFGAIELFSGLRNNIEDNVAHFAHLGGMLFGIILILLWKKQDRKRNVF
                     MP"
     gene            complement(6589..8403)
                     /gene="mutL"
                     /locus_tag="PK281_01015"
     CDS             complement(6589..8403)
                     /gene="mutL"
                     /locus_tag="PK281_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HOY47946.1"
                     /translation="MADIIQLLPEHVANQIAAGEVIQRPASAVKELMENAVDAGAKSI
                     QLIIKDGGRTLVQVIDNGCGMSEPDARMAFARHATSKIRQADDLWAIRTMGFRGEAMA
                     SIAAVAQVEMRTRLTGQELGTLLEIEGSEVKRQEAVACPNGTSIAIKNLFFNVPARRN
                     FLKSNPVETKHIFDEFQRVALAFPDIAFSLNNNGQEVYRLMPGTFKQRIVGLMGQSWN
                     ERLIAVKEETSVVTITGFVGKPDASRKTRGEQFFFINNRFIKSTYLNHAVQGAFQGLI
                     AADSFPVYFLRFDVDPKTIDINIHPTKTEIKFEDERSVYAILRSAVRKAIGAFSLSPT
                     LDFEMEQAFEIPLPNKREIKAPEIQVNPDYNPFSNPRPAPVEKQDQLRKLSQARWEDL
                     AGPPTELTFPPKKELSKPAETPEKTQPWQLHGKYICTQVRSGLMLIEQTGAFERIHFE
                     RLLKQKAESGHHGVSQQLLFPVVKEFSSPDFELIRALEPELRALGFDFENFGKTAIAV
                     NGVPPEVQELDIKNLFDSLLEQFKRSQQDLQLNRAEQLAKTLAANAARSASGSLSPEK
                     MLVLIDQLFACESPAYTPSGRSIISIIPLEELDARFGR"
     gene            8704..9870
                     /locus_tag="PK281_01020"
     CDS             8704..9870
                     /locus_tag="PK281_01020"
                     /EC_number="3.1.-.-"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: protein motif:HMM:NF013755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HOY47947.1"
                     /translation="MEQFSSYQYIQNQASMLQAAVVLRSCKEIAIDLEFDRDRYAYGF
                     TLCLIQIHGKGISYLFDPFVGLDFGPVFDILEDETIVKVLHSPGEDLQLLHKMGCFPK
                     NMFDTERSARILNYSAFSLSNLLNDILGIQLDKSQQKSNWTRSPLSETQLKYAASDVA
                     HLISLKEELQMQAMQKDVMDWLKEENAAWDDYRVEEKPDGHFANKEDSKKLPPFQLHV
                     YNAVLGLRDKIAQELNKPGYQVIPKELAMDVVFKPELVEDWMNQKGLNPRVKTLDFKR
                     QLKQIIDIAQQEATDRNLLKRNPGSHLSPAQREELANERVRIAALVDSGYRKVWQVIA
                     DRYGEFTAAYFLNERTMTDLTTGALKLEDLPYRYRAKLIREISLELELSELIKN"
     gene            9928..10221
                     /locus_tag="PK281_01025"
     CDS             9928..10221
                     /locus_tag="PK281_01025"
                     /inference="COORDINATES: protein motif:HMM:NF024232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3817 domain-containing protein"
                     /protein_id="HOY47948.1"
                     /translation="MQLNNSLNRFRWVALAEGWSFLFLLFIAMPLKYVAHMPMPVKIA
                     GWIHGLLFVLYGFTLIHVWADRSWKFSKALLAFLVSFIPLGTFWFDKQLKAES"
     gene            10267..12444
                     /locus_tag="PK281_01030"
     CDS             10267..12444
                     /locus_tag="PK281_01030"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HOY47949.1"
                     /translation="MKYIFTFLILFIGFNCFSARWYVHANASGANTGLSWTDAYTDLQ
                     SAIGSSTFGDEIWVAAGTYKPTSTTSATIYFNLVNGVAIYGGFNGSETLLTERDFVNN
                     ISILSGNIGSGSSTTDNSNHIVYGDNVGSQTRLDGFRISDGHASSLAGGVYLSSSSPT
                     ISNCTFINNFALDAAGALTHNGGTLTLTNCVFLSNFTDGTGGALQIRSGTARITDCRF
                     TSNQAGDNGGAMYLSGGVSYIDRCIFDGNSAQNNSGIIYHLYDNSMYLSNSLVVGNYA
                     PQYSIMYMPPISNSQMHGIVNCTFANNRQTQNSGQAYPLAVNSNTEIANSIFWDNGGA
                     AAMYPNADPHDCLIQGGFSSGTAIISGNPSFIQAGLTTLAPFELDGFDYHVSATSPVI
                     DKGVNNLVVAPADKDLDLADRIFGPRADIGAYELSYCNLPVTIETESTIPFCPQTTQT
                     LTASSGDNYTWYQGTNSIGTGNSISVTTGGTYVLQAADAEGCRGGDTIQVTFSSISFL
                     ITGTTTFCSGESTTLSLSGDYSNPVWNNSVNADQFVATNSGNVSVTAQTSDGCDVQLS
                     TNVTAVALPNPNINWVSNHLNAGSGFSSYQWFLDGTSISGATSANYTPTANGDYTVLV
                     SNVSGCENTSNVYTFSSLGIESVNSNWKMYPQPAGDLLFIEGLKPESNWKLINLCGQV
                     VLSGISDANSIFSITLTNLPAGVYFILFEQAGAEVITAKVVHQ"
     gene            12484..13287
                     /gene="proC"
                     /locus_tag="PK281_01035"
     CDS             12484..13287
                     /gene="proC"
                     /locus_tag="PK281_01035"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HOY47950.1"
                     /translation="MKITLVGCGNMGLIYARAFLKYAVVSADELLLVEKNETRVEELV
                     KMNLGRVVTPNNPEISKSDLLLLAVKPQDFPSLAAVLKHQLKPEISVISIMAGVTLSF
                     LEEQLQHVTLIRAMPNSPAGLGMGVTAMIAHPSVSSAHIRKAEQVLSTTGRTVFLDQE
                     SQMDAVTALSDSGPAYFFYLVKHMIEAGKAMGLEDSISAMLVKQTMLGAFHLINQADE
                     SLDNLIKAVASKGGTTEAAFKVFDDRALGQSLQEAIFAAEKRAGELSKS"
     gene            13384..13848
                     /locus_tag="PK281_01040"
     CDS             13384..13848
                     /locus_tag="PK281_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013186101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HOY47951.1"
                     /translation="MSKGADIDNLDRKILTILMNDAVVPYTEIAKQLDVSAGTIHVRM
                     KKLSELGVVLGSQLIINPSVIGFDICAFIGIFLDKGSAYNDARQQMMDIPEIVELHYT
                     TGVYSIFAKVLCRDTSHLREVLNEKIQPIVGIQRTETFITLEQGFSRQINII"
     gene            13951..14850
                     /locus_tag="PK281_01045"
     CDS             13951..14850
                     /locus_tag="PK281_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY47952.1"
                     /translation="MKKSLLFAALNLGIGTANAQLVSKKGEAYLPEPGDWSVGVDATP
                     FLNYAGNFLSSAGNSAPTQNFLNATQTIIGKYFVSEKMAYRGILRIGMGSQHWSENIA
                     QHGATAPVYPALPALVEDTYKEKSTNVGLGGGIEWRRGSTRLQGYYGADLMLTYTNSS
                     REYTYGNALNDSTSVTAISTSWYGQSSYPQNFVNDTYGNTARVTEEKDGSMLAFTLRG
                     FGGVEYFIFPKISLAGEFGWGISFANSSGGSQSIESVNFQSIDPSVGTQTIESNKSGR
                     FSLDTDRNLTGTSTGALRINFHF"
     gene            14986..15777
                     /locus_tag="PK281_01050"
     CDS             14986..15777
                     /locus_tag="PK281_01050"
                     /inference="COORDINATES: protein motif:HMM:NF025024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HOY47953.1"
                     /translation="MSSIIPSAFFEPSNLQALRNQFDAGKPFRHLVIDNFLDPEFANT
                     LYENFPGFDLMSRNYHGLNEHKSEGSGFENFHPAFNQLRAELNKPEFYKTLSTITGID
                     NLYSVEDALGMGVHQGGKDSYLDVHIDFNIHTARNIHRRCNLLIFLNKNWQESYGGHV
                     ELWNKDVSKLEQKVLPILNRCVIFETNEISYHGYSKINVPEGETRKSFYSYFYTDLRE
                     DAARFHDTVFKARPEEGIIKKVKTDVKETLKNTIKRNLKKVGINI"
     gene            15777..16538
                     /locus_tag="PK281_01055"
     CDS             15777..16538
                     /locus_tag="PK281_01055"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOY47954.1"
                     /translation="MKQEAIQYTQYTEYPCHLSRLEWIYDTIDQGRKPSSKVQVLDVG
                     AGTGNVTIPLGTIENSDILGIDIHQPTLDIAIEANKLPNVHFKFEYLQDSPLAGKEYI
                     ILTEVLEHIGPYPEILDYLGKNSDKNMKLLITVPNGFGPFEIAMQPLYFMRKIGLNNF
                     ILKVKRILGKKEPYSQNYETPHVNFFTVGRMRRELKQYGLKITEVKKAYVFAPIIETY
                     LPFIPLKGIARLDNKLASILPAFMASGFYFKIEHE"
     gene            16802..17719
                     /locus_tag="PK281_01060"
     CDS             16802..17719
                     /locus_tag="PK281_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY47955.1"
                     /translation="MQAKQSIFFFLLILLLSASCKKASSDLYTDNDPPAFSAVPAVKV
                     RNFVNRAFIDLIGREPTDAEMEANTAAIQSGNSNMEAREALIDKLMSDTTFRDGDTSY
                     TLAYHKRIYDLGKIRFMDGVSEAQLIQQAGIYRSNAISDSLSGNVPGYEEDMMRYNRM
                     KDVLLSARRYMQGEITIQQQFAAMINNAIYDIINMNSFNFVNACFNDLFYRFPSNAEF
                     QIAYSMVEDNQPGVLFNATAQNKDEYVTILTQSQECRQGIIIWSYQTILARNPVSAEI
                     YDEMLLFGQDGDFKALQKRLLKSAEYANF"
     gene            17763..18221
                     /locus_tag="PK281_01065"
     CDS             17763..18221
                     /locus_tag="PK281_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY47956.1"
                     /translation="MQFGLSSCKKEIEPDQYELNQVVLYPSISAKNKLKSQEQFVSIL
                     YTNLFQQALSGNKLVEIIDCLESIGDKELGREVLISNFMNDPAVQLPSDSTMRANPEA
                     FVAETYERFFVREPSQAELTWFSNFINAHPQLSAEMIYFSFALSNEYLYY"
     gene            18228..19571
                     /locus_tag="PK281_01070"
     CDS             18228..19571
                     /locus_tag="PK281_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY47957.1"
                     /translation="MNRRRFIRAAGLTTAGAFAAPYILPSGRLFASSGQPLAGHVVYV
                     LFAGGVRQQESVLQRYLDDSQGLPWEGNIMYNMLNGDAPQNKIVYGTDQGGGAEGGTP
                     IPRILNQTLQTQGLLFKEVSSQSNGHYGGLNNLLQGSTVTTQGLKQKPVNPTIFEYLR
                     RHGGYKATDAWFVGNGIGNSIPLLNYSNHPDYGSKYGANFFAPSVTFGNLGFEHLSNA
                     KVYHPENELDPMYQMKYFLDQSFSSVGQGGDSLGNTEEEKQRIKQFMRDTYERVTQGL
                     IAFPPVTDNSDSLNIGFACEVMREFQPAITVVNLSDVDGCHSDFTGYLRALHRADHAV
                     GHLWNYIQTQIPGMANDTVIIASPECGRNLQHNPIKDVNDWYAYDHSDFNSSRVFTLM
                     AGPSVPSNLVLGSENNSIGFTSDNVPTIAEILGVKDEVMNAGFLQSGARSLFERI"
     gene            19568..20506
                     /locus_tag="PK281_01075"
     CDS             19568..20506
                     /locus_tag="PK281_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY47958.1"
                     /translation="MRKALILFLCIGFFSCKKEQEENPYDLIQNPNDTSGTHHTVPDP
                     NSLAGIHQNIFEPTCANSGCHDGTFEPDFRTPESTYNTLVLRPIIKNDPAGTYTYRVV
                     PGDYLNSVLYQRLIIDIDGQSGIMPLVLEPSSDWNSKQAQYIENVKNWIQNGAKDIFG
                     NSAANFNLPPQLLGIFISETGSTQAFPRNATSGSIQIPIGTSSIDIWLAIEDDQTTID
                     QLSYLKAKVGASMNDFSAVSEQNLQLVNEVMETGYSGNQVSFRFKFTLPLSGLTPVQP
                     WFIRAYIQDATASPSELPSTGSAEYIKHFYSFQLGE"
     gene            20503..20949
                     /locus_tag="PK281_01080"
     CDS             20503..20949
                     /locus_tag="PK281_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY47959.1"
                     /translation="MMRNMLFLFFLVVLWSSCTEKERVPAPAPPAIGIISITPDSILQ
                     FQDSAFVTIQYEDVNGDVGSSDPDAKDLEVKDSRLAQPDLYSIQPLTPDAIELFIHGQ
                     IKIKLPTLFILGNGDYETVTFSIRLRDQSGEWSQTVTTDPIVIKRQ"
     gene            20946..24458
                     /locus_tag="PK281_01085"
     CDS             20946..24458
                     /locus_tag="PK281_01085"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOY47960.1"
                     /translation="MSFIRKYGFLFLLLVCGFNPVCAQLEFNMSDTLVTECKGILYDD
                     GGDGVNYFHNANETFTICLDAPGTLNLAFDFLCIELGSDSLMFHAGSSILDPQIGPAY
                     SGTTPPPVHTINSGCITLHFVSDANVACTGWEAHWTTQIVPPVPPLIAAIIPVPACSS
                     STAIINLSKNIACDSVYAAAFELIGPLNVPILSASPISCTNDSTQQVQITFASGIDLG
                     GNYQIEFTTHYWDACDSLWTFTTSDNFSVFDCPIVVTLTPQNDSICFGDCTIIDVLVT
                     GGDGNYTYSWNTGMSYAGPAQTVCPLNDITYTLTVDDTSPALASSGSTSITVFQPATV
                     PAPSSHCQSDAPFDLSASPTGGWWIGSGITDSLAGTFNGDSAVLGLNQPGYYLPITPS
                     FGCTTLTNITIIPINAGIAQAACPGSAPFQIIGFSPIGGTWSGLSVSPSGVFDPVAAG
                     VYTVTYSVNGCSEDLTIYVDDISNLPVLTDTICQSAASILYALNPPGGRWTGEGIIDS
                     LTGLFDPGETDAGLIPITYNLNGCSQTIEVYVKEIFAGWDQTACPSQDTFLLADFSPI
                     GGTWSGTGIVDSLLGIFEPGTNTNQNFTTDLIYSHPNGCTDTTTIYVVYSRIYRDTIV
                     FCSGDQGIILDHFSMETSPWSGNWSGNGVIPGADQDSSFFDPNIAGTGVHLLTFDINT
                     CADTAYMIVQKAFLPDIPLTCEQAPAFQITVPEYALGGTFSGDGITDSIGMFSPSDAG
                     TGLHEIYYESPQGCLDTLIAEIDSYQEASIDGPAGVLCYTDSLYSVTILPLDALYYGT
                     GSVLPGSFNPIISGEGDHWLYAETGQGYCRSIDSVMVSVGPAIGYSLVVSTDSICYGD
                     YVSMQANAFGGNGNLITYTWNQGLQPLQQQVVSPLVSTQYTLEISDGCSRLRDTVDIT
                     VSDPITYSISTSDSLCYGRPGFAQIDGGHSSSYQVVWDNQLYITGEQLPGLASNTYSV
                     SIRDTVSGCSVDTLITIPGFPLVKAQFSVNPEYDCIPEEIKDINFIDLSSGAFQGSWN
                     FGDGTVIPYVSGENPTHSYPIYGEYVVGLEVADSNGCSDKTDRTICLKEPFKVYLPNA
                     FTINEDGLNEVFLAKGTGILTFHMWVYERSGKVLFESDALDKGWDGKLNGRIVPMGVY
                     GWIVEVQWTDKQWFTTAGTVTLIR"
     gene            24489..25919
                     /locus_tag="PK281_01090"
     CDS             24489..25919
                     /locus_tag="PK281_01090"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOY47961.1"
                     /translation="MLPAQHFPFIGNQYRTQVSGLDLLVYHKHTNELLARIPQIGLNQ
                     VEEAIASTISGFQEMRKLDISERAAILQKIRDLLDERKEEFIQLIVAEAGKPISFARQ
                     ELDRCLLTIDTGIREVWSLNGEQIPMNAGSGKGKIAFTQQFPTGPVLGISPFNFPLNL
                     ALHKIVPALAVGTSILLKAPPQAPLSVLGLAGLIAEAGAPAGAVNVIVCDIPVAEKLV
                     SDSRFSVLSFTGSARVGWHLKQIAGNKKVLLELGGNAPVIVDETADLAEAARQIARGA
                     FLFAGQICISTQRIYVVESVADEFRDLLLSETSRIRSGDPSDEYTINGPIIDANTIQR
                     IQEWLQEANRSGAEILAGGNLIDAECRIFSPTILTRTTPEMKLVKEEVFGPLAIFEII
                     RNLEQGVDLANASEYGLQCGVFTNHLERIKYAYQKLEYGAVIINSAPGFRVDQMPYGG
                     IKSSGLGREGIRYAMEAYTEKKLLVF"
     gene            26032..27930
                     /gene="acs"
                     /locus_tag="PK281_01095"
     CDS             26032..27930
                     /gene="acs"
                     /locus_tag="PK281_01095"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HOY47962.1"
                     /translation="MSFHRIRTLEEYQHAYEESVKNPEGFWADIASRLTWKKKWDTVL
                     EWNFDEPSVKWFSGGKLNITENCLDRHLDTEGNKIALIWEPNDPKERAKKFKYRELYV
                     LVCRFANVLKRNGVRKGDRVCLYMPMIPELAIATLACARIGAVHSVVFAGFSAQSIAD
                     RINDSACSVVITADGMSRGPKQLPLKRVVDEALEQCASVNRVIVHNQLNWDPEMIEGR
                     DVWWHEEMEMVDSDCPAESMDSEDMLFILYTSGSTGKPKGVVHTCGGYMTYIDYTFRN
                     VFQVESNDVFWCTADIGWVTGHSYLLYGPLMSGTTTLMFEGIPTYPDAGRFWSVIDKH
                     SVSVFYTAPTAIRSLMAAGVDHVLAYSLNSLRVLGSVGEPINEEAWQWYNVHVGKEKC
                     PIVDTWWQTETGGMMISAMAGITPSKPSFAGFPLPGIQPVLLNAEGQEIQGNGEEGYL
                     AIKFPWPGMIRTTYGDHERCRVTYFSTYKNYYFTGDGARRDEEGMYRIIGRVDDVINV
                     SGHRFGTAEIENAINQNKHVIESAVVGFPHDIKGQGIYAFVICDKLPDHPVQLREEIY
                     ETVSEQIGRIAKPDKIQFVSGLPKTRSGKIMRRILRKVAENEIGNLGDVTTLLDPLIV
                     DEIIAGKL"
     gene            28283..29449
                     /locus_tag="PK281_01100"
     CDS             28283..29449
                     /locus_tag="PK281_01100"
                     /inference="COORDINATES: protein motif:HMM:NF019268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOY47963.1"
                     /translation="MKNKVVAAALMVILCNTVGWSQVKNDTISPTVKDLTFTGTRINP
                     YDAETDTSGKIEFSGYVDAYYACYTDTASVSTFQKFPTIAPRSNSFGLNIAQASARYS
                     SPRFRGVITVFAGDVARSAWSPEFSFIQEANAGFRIGKKVWIDAGFFRTHLGLESIQP
                     RENITLSIATTTYFEPYYLSGAKLTWELNSKLTLQANVFNSFNGFVENNRNKALGFSM
                     AWQPIKGLSFTYGNILCEETPGELYGKKTRFYNNLFAVYKRRRITLGAEVNYGTQNHT
                     GINDSQHLAYMFSSLLAFKYRLTPQLALYTRGEYFADPDEMLTGPVQNEYHQLVGLRV
                     GGITQGIEYKPIPNSYLRLEGRWLDARKDEHIFLLRNAPSHNRYELLGGLGLWF"
     gene            complement(29505..29930)
                     /locus_tag="PK281_01105"
     CDS             complement(29505..29930)
                     /locus_tag="PK281_01105"
                     /inference="COORDINATES: protein motif:HMM:NF024275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY47964.1"
                     /translation="MKKSIKIILAVLLVGGAIGGIVAYRMWNKPHADASEMEGVKVTA
                     DVLYKAFESNEQQANSTYVGKVVEVTGTVGDIEKSDTISRVMLTFPDAMMGAVRVTLD
                     TRHLKDADAVKSGEQATFKGFCNGYLMDVEVKDGVLVKE"
BASE COUNT         8813 a         5814 c         6290 g         9138 t
ORIGIN      
        1 caaacgcaca agagtagaaa agatacgttc aaaaggctga ttaagaatcc acttttgctt
       61 gtatttgggt atgataacta atgcaggatg gccagcttga ctgagatatt taggcagcgc
      121 gccaaccaca tcacctaatc cgccagcttt ggcagcagga taacattctg cacttatgtg
      181 tattacttgc atagaatagg ctaagcggta aagttaaacg gcaaaaggga attacaaata
      241 cccgaaaatc aaattattat taaggtatta aattcgagtt attcctcccg aaacaataaa
      301 cttcatgatt tcatcggaag gtgcatcaat aggggtgatt agatcattgt cgacaataaa
      361 aagattaccc gaaaaattat agctgtgcgg taaataaaca gccgattttc cttctttaat
      421 tcctaaataa gtgaggtctt tttgtgtgac aaagcccaat ttgtataaat cggcatcacg
      481 tccaactttc accatgactg gtttattgaa acgtttttca ttcccaacaa aagccgcaag
      541 catatctcta accgaagtat aaattacttt gattaaagga actcgatcaa tggttttatt
      601 aaagagtgat tttagtggtg tttgaatgag agctgtacca ataattccga ggaaagttgt
      661 tcctgcaatg atgatcagaa tactaactcc tggaacttcg tatggaatta gtccatccaa
      721 gaacataaat accttataaa ctacataaaa ggtaatacct aaagggacaa tcagtacaag
      781 tcctttcagg aaataactgg taaaccggcg aaaaatatgt cggaaaaatt tactgagcgg
      841 attcattttt caaggaataa gctgcaaagg taaattaatg caagcgatag ttagtgacta
      901 actaccttca atccaatgat ggagcaaatt agtgttccga gaaagaaaat cctccaaaat
      961 tcagctggtt cgcgaaaaac aattaatcca actagaacag ttccaactgc gccaacgccc
     1021 gtccatactg cataagcggt tccaataggt aacgtttgtg ttgctttgta tagtaatagc
     1081 atactgatgg ttaggcacac aaaaaatcca agcatccacc aagtagctgt ttgaccggtg
     1141 ctttctttcg cttttccaag gcaagttgcg aatccaactt caaataatcc agcaataatt
     1201 aagataatcc aattcattta aggctctcct tcacttttcg agtaagtcca gcatacacta
     1261 aatcgtagga ataatgtatg agttctttca ccaattcggc aggtaaatgc tcaaaatgaa
     1321 gtgtattcca attggtttta ctcatgtgat aaccggcttc gatggctgaa tattcttccc
     1381 gtaaactgat ggcacgttct ggatcacatt tcaaattcat ttgtccacct ttttccaatg
     1441 gaattaatgt aaaaattttt ccgccaatgc gcatcaccag cgtaaactca tcaaatggaa
     1501 aatcttccgt cacatgaggt agattgaggc aatattcacg gacttcttcg atgttcattt
     1561 ttttgatgtg ctgatttgct aatgtgctaa tgtgctggtg tgctaatgtg ctggtattct
     1621 gatgagttga agtattaatc gtactttaag ggaagtgttt tggattgttt catttcggag
     1681 atgatgagca tggtttctac atggcggact tcttccattt tagagaattg gtcggcgatg
     1741 agtttttcga atgcgggaat atcttgaacg agcactttca atagataatc ggaattaccg
     1801 gtaatctgat agcattccat gatttcggga attcccatga tgcgatcgcg gaatttattg
     1861 atcacatttt cgacttgacg ggcgaggctg atttcaataa acacgctaac acccaatttc
     1921 aacttggagc gattcaagcg agcatggtag ccatcgatga atttttgttt ctccaatttc
     1981 ttaactcgtt cgagagttgg ggcaggggaa aggccaattt gattactcaa ttctaggttg
     2041 gtcattcgac catcttcctg aagtagtttc aggattttat aatcgattgc gtcaagttta
     2101 tctgcgggac tgctcatgtg ctaaaattac aaaaaggaaa atggaatgag catgcattcg
     2161 catcaaattt acttattgaa ataagcgtga agcaattcca tctgccaata accaagcatc
     2221 aggatttaat cgaaatgagg tttcggtttc aatgatatgc tcaggatttg gtagctgttt
     2281 tcgagccgat gaaaagtact gaaccgcttc ttttccaaac ttaattaagg ctgctttatc
     2341 aattccattg agcgtgcgta gtccggttaa taccagttcg ttgtaacgag ttttttcatc
     2401 tattacttcg agcgtatgtt cgcaatctcc ttgagctaaa tcgcgaatat atcggttgtt
     2461 gtttgccaca ttttcgcgtc gaatttttcc gttaaatgaa tgagctgaag gaccaattcc
     2521 cacatacgat tttcctgacc aataggctga attgtgaacc gcaacatgtt cgccccgcgc
     2581 cagattactt atttcgtaat gcaagaatcc taattcggga gctaacgatt gtagaatctc
     2641 aaattgtcgc gccgcttgtt cttcatctgg attagggtaa ttaccttttc gaattccttt
     2701 agctaaaggc gtgttaggct caacagttag tgcataacat gataaatgat taacgggtaa
     2761 gctagccgct tgtcgtaaat tggaaatcca acgttcatcg cttaatcccg gtgttccata
     2821 aattaaatcg atggaaaatt ctttaaatcc aatttctgtt gctaatgcta tacaattgag
     2881 tgcttcgttt gcagaatgag cgcgattcat aaaacgaaga tcttcctcaa aaaacgactg
     2941 aactccaatg ctcaatcggt ttattccctg atctttaagt tgaagtagtt tttcgcgact
     3001 taaatcatcc ggattagctt ctaatgttac ttctgcattg gttgctattc ctagatttga
     3061 gtgaatataa tccatcaatc gactcagttc cgaattcgaa agttgagaag gagttccgcc
     3121 accgaaatac acacttttga gtggaagaat aaattcactt ttccggaatt ccatttcttg
     3181 tattaatgcc tctaccatgt gtgatttgta atgtgtagaa gtagtgaaat ggaaatcgca
     3241 atacgtgcaa gcctgtttgc aaaatgggat gtggatgtag agcacgtaaa actgattgga
     3301 agaaaatact taagcttttg gaagtaaaat gcggaatgtt gttccttttc ctacttccga
     3361 acggcgtacg aaaattttgc ccttatgata atcttcgatg atacgttttg ctaaggataa
     3421 acctaatccc caaccacgtt tcttactggt ataacccggt tggaaaactg ttttaagttt
     3481 tgatttagga ataccttttc cagtgtcgat tacgtcgatg taagcgtatt tccctttgtc
     3541 gccaaattga acttctaaag aaccacttgt ttcgatggca tcaatagcgt tgcgaaagag
     3601 gttttcgatc acccaatcga aaagcggaac attgagttta acgttcatgc gctcatcttc
     3661 aggtaataaa ttcaccaaat cgatgttgat tttacgtgaa gttcttggac gcatataact
     3721 gatactttct tcaagaactt gctgtagatc ctgtgtttta agttctggct tagaaccaat
     3781 tttggagaat cgttcggtaa tgatttcgag gcgcttgatg tctttcccca tctctccaag
     3841 cgtttcctcg tcgatgcctt tcatgcgaag gatttcgatc caggcaatca aactcgaaag
     3901 gggtgtacct aactgatgcg ctgtttcctt cgacattccc acccaaacct gattctgctc
     3961 tgctcttcgg gagaaattga acaggaaata agcgagaatc atgaataggg caattaccgt
     4021 gaactgaatc accgggtaat acttcaactg cgttaacaaa aatgagcctt ggtagtatat
     4081 gctgcatttt ccgtaatcag gaagttcaat ttcgagcgga gtattttctg aagccatgct
     4141 agtggttagc tttagcatag ctgtggtgtc tgcgatgatg gtcgaatcga ggttacccga
     4201 agctataacg cgtgtttttt tcttatcaac cacaattact ggaacactcg ctgagttgac
     4261 aactacctca gatataaaag atttgatgat gttatcgagt acttctctta actcactgaa
     4321 tacgcgtgaa tcctggtagt aaatgaaaaa tgtattggaa aaaccatcgt caattacaat
     4381 cggacgatgt gctgaaaatg ctgcttttac ttttccggtg aagaattctg cttttccaaa
     4441 tccggttgta tcggcattag tgatgtagtt gacccggttt tgttggtcta ctattatagc
     4501 tggaatcgta ttgttttcgc tgaccacttt taggtagaac gaacgtgcat catcgtcttc
     4561 ggtgatactt aggttttggg tggcttcggc ccaaatttcg gcacgtttgc gttcttcggt
     4621 tttaagtcgg tcgaataaac tctcggtgta ttttaccagt tcggcgcggt tgcggacggc
     4681 atcagcccag agttctacct ttttgcgttc ttcggtagat acttttttga caagcacatt
     4741 ggtgtaccaa agtgatacag aaataatgat gacagccatt cctgccagcc ataatttcca
     4801 ccgatatttt ttagcgtaga tattcaagtt acttgtcctt gctgagttcg aaaagtaggg
     4861 ctttttcttc tttgctcaga ctgtcgtacc ccgaacgaga aatcttgtct aaaatttcat
     4921 caatacgttt ctggcgaatt ttagagttga ggttccaatc ttcatctttc attggacgct
     4981 tgtgcgcaac tttcagattg gatttcctgc ttttttgaaa agaaagtaaa tcctgtattt
     5041 tgccaaaaaa ggaggctaaa tctcttcctt ttttcaaatt catcgcccaa ataaatccga
     5101 aaagagcgcc accaagatgt gctagatgtc cgccttcgtt accgttactg atggaaatta
     5161 aatcgagtaa gacaaataat aaagctagcc aacgaagttt catcttgaat gcaccaaaca
     5221 agaacacttc atcatcgggg cgataggtgc aaatggcaat caaaacgccc attacagctg
     5281 ccgaagctcc aacagccgta tagttaatcg gtaaaacatt gaaaagtgga aatagattta
     5341 ccgaaagcag gaatacgaat gctccagcaa aaccacttac aaaatagaca ttgataaatc
     5401 ttcggtcatc gaaatacctg ttgaacaatt gataggtaaa aaacagggtg agcatattga
     5461 atagaatatg catcaatcct cgatgaacaa agaggtgagt gataatcgtc caaggtttga
     5521 agagcagctt tagcggatct ccggatagtt cgagaatact taaaattagt acgccatcag
     5581 catctcgatt aagtaaatgt aaaatcgaaa tggggacgaa tactaaaaca aaagcaatta
     5641 cattaataat gatcaaccga acaccagcgc ccgaagttga ccaaagaata cgtgcctgac
     5701 tcatggcatg aaaacgttac gcttgcggtc ttgttttttc cagagtaaaa taagtatgat
     5761 tccaaacaac attcctccaa ggtgagcaaa atgcgctacg ttatcttcga tgttgttacg
     5821 taagcccgag aataattcaa tagctccgaa aaccataacg aaatacttgg ctttcattgg
     5881 aataggagga atgagcatca tgatcatcac attagggaag atcattccaa aggcaagtag
     5941 caaaccatac aaagaacctg aagctcctac attgaccgtg ttctggcgaa attcaacaac
     6001 ttcggcaacc agctttttgg attctactac ataacctgga ttttttaaat cataatacca
     6061 agccttcatg aattcgttca gttgctcgtt atcgtatgaa ccgggaagat aattcgtagt
     6121 aaaacgatcg aaactgatgt aattcggatg catgttataa gcatttgctg cttctaacat
     6181 gggacgaaat tgaaaattag ttacagttgt ttgaacggtt cctgcaccaa ttccagtaat
     6241 aaggaaatat attaaaaagc gtttcgaacc ccagaaacgt tcgagacttg aaccaaacat
     6301 ccacaatcca agcatgttaa agaacaagtg gaatggttct ttggtatcgt gcatgaatag
     6361 gtgcgtaaca agctgccaag gctgaaaaaa gggtgagtcg aaattaaaca gtccgaggta
     6421 gcgacttaaa tcaattcccg attcaccagc tccaatggac aaaagccaaa gtgctacaca
     6481 aataattatt aacgtacgta caacgggagt aatcgaataa ggagatcctg aattcatttg
     6541 gcaaaagtag ggaaatgcgc atttgggaat aaggaaagaa ttcggaaatc aacgaccaaa
     6601 tcgtgcatcg agttcttcaa gcggaataat ggaaatgatg gatcgtcccg atggtgtgta
     6661 agcgggtgat tcacaagcaa ataattgatc gattagcacc aacatttttt caggacttaa
     6721 gctgcctgaa gcagaacgag ccgcgtttgc cgcaagagtt ttagctaatt gttcggcgcg
     6781 gttgagttgt aaatcttgtt gactacgttt gaattgctct aaaagagaat cgaacaggtt
     6841 tttgatatcc aattcttgaa cttcgggcgg aacgccgttc acagcgatgg cagttttgcc
     6901 aaagttctca aaatcaaaac ccaatgctcg aagttcgggt tcgagtgcac ggatgagttc
     6961 aaaatcggga gatgaaaatt ctttcaccac cggaaaaagc aattgctgcg atacgccatg
     7021 gtggcccgat tcggcttttt gcttaagtaa acgttcaaaa tgaatgcgtt cgaatgcacc
     7081 agtttgttcg attaacatga gtccactacg aacctgcgta caaatgtatt ttccgtgaag
     7141 ctgccaaggt tgagttttct cgggagtttc ggcaggtttg ctcagttcct ttttgggtgg
     7201 aaaggtgagt tcggtgggcg gtccagctaa atcttcccaa cgtgcctgac tcaattttcg
     7261 caactgatct tgcttttcga ctggcgcagg acgcggattg ctaaacggat tgtaatcggg
     7321 atttacctga atctccggag ctttgatttc gcgtttattg ggaagtggaa tttcgaacgc
     7381 ttgttccatc tcaaaatcga gcgttggaga aagcgagaat gctccaattg ccttgcgaac
     7441 agccgaacgt agaatggcat aaaccgaacg ttcgtcttcg aatttaattt cggttttggt
     7501 cggatgaatg ttgatgtcga tagttttggg atcgacatcg aaacgcagaa aataaactgg
     7561 aaatgaatca gcagcaatta aaccttgaaa agcaccttga accgcgtgat tgaggtaagt
     7621 actttttata aaacggttgt tgatgaaaaa gaactgctct ccacgcgttt tgcgagatgc
     7681 atcgggctta ccaacgaaac cagtaatggt aacaactgag gtttcttctt taactgcgat
     7741 aagacgttcg ttccaggatt gtcccatgag tccgactata cgttgtttaa acgtaccggg
     7801 catgagtcga tacacttctt gaccgttgtt gttgagcgaa aaagcgatat cgggaaaggc
     7861 aagagcaaca cgttggaatt cgtcgaaaat gtgtttggtt tcgactgggt tacttttgag
     7921 gaaatttcga cgagcgggaa cgttaaagaa caggtttttt atggcgatgg atgtgccgtt
     7981 tggacaggca actgcttctt gtcgttttac ttcggagcct tctatttcga gtaaggtgcc
     8041 gagttcttgg ccggtgagac gcgtacgcat ttctacttga gcaacagctg cgatggatgc
     8101 cattgcttca ccacgaaaac ccatggtgcg aatggcccaa aggtcgtcgg cttgacgaat
     8161 tttagaagta gcatgtcgcg caaaagccat acgagcatcg ggttcggaca tgccgcaacc
     8221 gttgtcgatg acttgtacga gcgtacgacc gccatctttg atgatgagtt gaatggattt
     8281 ggcgccagca tcgacggcat tttccatgag ttccttaacc gcacttgcgg gacgttgaat
     8341 tacttcacca gcagcaatct ggttagcaac gtgttcggga agaagctgga taatatctgc
     8401 cataagcggg cggcaaaggt aagcaggata gcagtttttt ggagcataat ctaaagagct
     8461 gagattagaa agtgcttgag tgatgcctga atgatggaaa tgaagcaaga agtggtaagg
     8521 atagagcgtg caggcaggga agataatttt atgaaccggc ttgttgaaaa tttgtacttg
     8581 aacagcttga atgtattaac tgcctgatag cgatagcctg ggcaccaccc aaaaaatgaa
     8641 cttgaatcag tcgaattcga gatggaaatg aattacgggt atattgcgaa ataaattgaa
     8701 tttgtggagc agttttcatc ttatcagtac atacagaatc aggcttcgat gctgcaagca
     8761 gcagttgttt tgcggtcgtg taaggagatt gcaattgatt tagaatttga tcgtgaccgg
     8821 tatgcgtatg gttttacctt gtgtttgatt caaattcacg ggaaggggat tagttatttg
     8881 tttgatccat ttgtgggact tgattttggg ccggtgttcg atattttaga agatgaaacg
     8941 atcgtaaaag ttttgcattc gcccggagaa gatttgcagt tgttgcataa aatgggatgc
     9001 tttccgaaaa acatgtttga cacggagcga agtgctcgaa tcctcaatta ttcggctttt
     9061 tcactttcga atttattgaa tgatatactt ggaattcaat tagataaatc gcagcagaaa
     9121 tcgaattgga cgaggagtcc gttaagtgaa acacaattaa aatatgctgc aagtgatgtg
     9181 gcacatttga tttcgttgaa ggaagaattg cagatgcagg cgatgcagaa agacgtgatg
     9241 gattggttaa aagaggaaaa tgctgcttgg gatgattatc gggtagaaga gaagccggat
     9301 ggacattttg cgaataaaga agattcgaaa aaactgccac catttcagtt gcatgtgtac
     9361 aatgctgttt tgggtttgcg agataaaatt gcgcaagagt taaacaaacc cggttatcag
     9421 gtaattccaa aggagttggc tatggatgtt gtgtttaaac ccgagttggt agaagattgg
     9481 atgaatcaga aaggtttgaa tccacgagta aaaacgctcg attttaaaag gcaattgaag
     9541 caaataattg acatagcgca gcaagaagct actgatcgaa atttgttgaa gcgaaatccg
     9601 ggaagtcatt tatcgcctgc tcagcgtgaa gaattagcca atgagcgcgt tcgaattgct
     9661 gcattggttg attcgggata tcgaaaagta tggcaagtga ttgcagatcg atatggggaa
     9721 tttacggctg cgtatttttt aaatgagcga acgatgaccg atttgactac aggtgcactg
     9781 aaattagaag atttgccgta tcgatatcga gctaaattaa tacgagaaat tagtttggaa
     9841 cttgaattat cggaattaat taaaaattga atttaaatct gacaaacgaa tgacattatt
     9901 taaccagtat aattcgaggt ttgcaacatg caactaaata attcgttgaa ccgttttcgc
     9961 tgggtggctt tggccgaagg ttggtcattt ttatttctac tttttattgc catgccatta
    10021 aaatacgtgg cacacatgcc tatgcctgtt aagattgctg gctggattca tggattacta
    10081 ttcgtgcttt atggttttac attaattcac gtttgggctg atcgaagctg gaaattttcc
    10141 aaagcgctgc ttgcttttct ggtctcgttc attcccttag gaacattctg gttcgacaaa
    10201 caactgaaag ctgaatcata gattcattgg gtttttaggt attttcgctt aagaaaccta
    10261 atacccatga aatacatttt tacttttctt atcctattta taggcttcaa ctgcttttca
    10321 gctcgctggt atgttcatgc caatgcatca ggcgccaata ctggattatc ttggacagat
    10381 gcctacaccg atttacaatc tgccatagga agttctacgt ttggggatga aatttgggtt
    10441 gcagctggaa catataaacc aacaagtaca acttcggcga ctatctattt taacttggtt
    10501 aatggtgtcg ctatttatgg tggatttaat ggttctgaaa ccctgttgac cgaacgagat
    10561 tttgtcaata atatcagtat tctcagtgga aatattggta gtggttcttc tactaccgat
    10621 aatagtaatc acattgttta tggtgacaat gtgggaagtc aaacacgttt agatggcttt
    10681 cgaattagtg atggacatgc cagttcatta gcgggaggag tttatttgag ttcaagttct
    10741 ccgactattt ccaattgtac gtttatcaac aacttcgcat tagatgcagc tggggcactt
    10801 actcataacg gaggtacgct cactttaaca aattgtgttt tcctttcgaa ttttaccgat
    10861 ggaacaggag gtgcattaca aattcgtagc ggaactgctc gcataaccga ttgtagattt
    10921 acatcaaacc aagctggtga caatggtgga gccatgtatt tgtcgggcgg tgtgagttat
    10981 atcgatcgat gcatttttga tgggaattca gcacaaaata atagtggtat catttatcac
    11041 ttgtatgata attccatgta tttgagtaat tcactagtgg taggaaatta tgccccacaa
    11101 tattcgatca tgtacatgcc gcccatttcg aactcacaaa tgcatggtat tgtcaattgt
    11161 acgtttgcaa ataatcgaca aacacaaaat tcaggacagg cttatccact agcagtaaat
    11221 tctaataccg aaattgctaa ttccattttt tgggataatg ggggtgcagc tgctatgtat
    11281 ccgaatgccg atcctcacga ttgtttaatt caaggaggtt tttcaagtgg aacagctata
    11341 atttctggta acccctcttt tattcaagca ggattaacta cgcttgctcc gttcgagtta
    11401 gacggtttcg actatcacgt ttcagcaact tcgcccgtga ttgataaagg tgtaaataat
    11461 ctagttgtgg ctccagctga taaagatttg gatttagcag atcgcatttt tggaccacgc
    11521 gcagatattg gtgcttatga attaagctat tgcaatcttc ctgttactat tgaaaccgaa
    11581 tctaccattc cattttgtcc gcaaacaact caaactttaa cggcaagttc aggtgataat
    11641 tatacttggt atcaaggcac aaattcaatt ggaactggta attctatttc tgttaccact
    11701 ggcggtacat atgtgcttca agcggctgat gcagaaggtt gtcgcggtgg tgataccata
    11761 caagttacct tttcgtcaat tagttttctc atcactggaa ctactacttt ttgctcaggt
    11821 gaatcgacca cattgagttt gagtggtgat tattcaaatc cagtatggaa taattcagtt
    11881 aatgccgatc aatttgtagc aaccaattca gggaatgtga gtgtaacggc tcaaacttct
    11941 gatggatgcg atgttcaatt gtcgaccaat gtaactgctg tggctttacc aaacccgaat
    12001 attaattggg tgagtaatca tttgaatgca ggttctggat tttcgagtta tcaatggttt
    12061 ttagatggaa cgtcaatttc tggggcgacc tcagctaatt atactccaac tgctaatggt
    12121 gattataccg tattggtatc gaatgtttcg ggatgtgaaa acacgtccaa cgtatatact
    12181 ttcagttctt tgggaataga atcggttaat tcgaattgga agatgtatcc tcaaccagca
    12241 ggtgatttac tctttatcga aggactcaaa cccgaatcca attggaaatt gataaatcta
    12301 tgtggtcaag ttgttttaag tggtatttca gatgctaatt caatcttttc aatcacatta
    12361 acaaacctac cagctggcgt atattttatt ctatttgagc aagcgggtgc tgaagtcatt
    12421 acagccaaag ttgtccatca gtaatttgtt tagctgatta attaaaatct cgagtacaat
    12481 cctatgaaga ttacgttagt tggttgtgga aacatgggtt tgatatatgc ccgtgcattt
    12541 ttgaaatatg cagttgttag tgcggacgaa ttgctcttgg ttgagaagaa tgaaacgaga
    12601 gttgaagaat tagtgaaaat gaatttgggg cgagttgtca cacccaacaa tcctgaaatt
    12661 agcaaaagtg atctcttatt attagccgta aaacctcagg attttccatc actagcagcg
    12721 gttttaaaac atcaattaaa gccagaaatt agcgtgattt cgattatggc aggagtaaca
    12781 ctttcctttt tggaagagca attacaacat gtcacattaa tacgagcaat gcctaattca
    12841 ccagccggat tagggatggg tgtaacagca atgattgcac atccatccgt ttcatcagct
    12901 cacattcgta aagcagaaca agttttatcc accactggtc ggactgtttt tctagatcaa
    12961 gaatcgcaaa tggatgctgt tacagccttg agtgatagtg gtccagccta tttcttttat
    13021 ttggtcaagc acatgattga agccgggaaa gcgatgggat tagaggatag catttcggcc
    13081 atgttggtaa aacaaacgat gttaggagca tttcatttga ttaatcaagc tgacgaaagc
    13141 ttagataatt tgataaaagc tgttgcttca aaaggcggga cgaccgaagc tgcctttaaa
    13201 gtgttcgatg atagagcatt agggcaatct ttacaggaag caatatttgc agcggaaaaa
    13261 cgcgctggtg aattatcaaa aagttaagaa agcgagcgtt tcgttaaaca taattcaata
    13321 tttatttata tttgcccgct tttataagga ggcattaccc aaattaattc atactaacag
    13381 aagatgagta aaggtgcaga tatagacaat ttggacagga aaattctaac cattttgatg
    13441 aatgatgccg tagttccata caccgaaatt gccaagcaat tggatgtctc agcaggaacc
    13501 attcacgttc ggatgaagaa attgagtgaa cttggggttg ttttaggctc acaattaatc
    13561 atcaatcctt ctgttattgg cttcgatatt tgtgctttta ttggaatctt cttggataaa
    13621 ggttcagcct acaatgatgc ccgtcagcaa atgatggata ttcctgaaat cgtggaactt
    13681 cattatacaa caggtgttta cagcattttt gctaaggttc tttgtcgcga tacaagccat
    13741 ttacgtgaag tgttaaatga gaaaattcaa ccaattgtag gaattcaacg tacggaaaca
    13801 tttatcacgt tggagcaagg attctcgcgt cagatcaata tcatttgatc attagttttc
    13861 tcacattctc ctgtttataa tctaagagtt gtagccgatt aacagaacat taatcggcta
    13921 aaatttgtgt taaaccaaaa tcgatttaac atgaaaaaaa gcttactctt cgcagctcta
    13981 aatcttggaa taggaactgc aaatgctcag ttagtttcaa aaaaaggtga ggcatattta
    14041 ccagagccag gtgattggtc tgttggagta gatgcaacac catttcttaa ttatgcaggt
    14101 aatttcttaa gctctgcggg aaatagtgct ccaactcaga atttcttaaa tgctacacaa
    14161 acaataattg gtaagtactt cgtatccgaa aaaatggctt atcgcggtat tcttcgtatt
    14221 ggtatgggtt ctcaacattg gagtgaaaat atcgcccaac atggtgctac cgctcctgtt
    14281 tatccggcct taccagcttt agtggaagat acttacaaag aaaaaagtac gaatgttgga
    14341 ttaggtggag gtattgaatg gcgtcgtggt tctactcgtt tacaaggcta ttatggtgct
    14401 gatttaatgt taacatacac taattcgagc cgcgaataca cgtatggtaa tgcgttgaac
    14461 gattcaactt cagttacagc tatatctact agttggtacg gtcaaagttc ttatcctcaa
    14521 aattttgtta atgatactta tggtaatact gctcgtgtta cagaggaaaa agacggtagc
    14581 atgttagcat ttacccttcg tggttttggc ggtgttgaat acttcatttt cccgaaaatc
    14641 tctctcgcag gtgaatttgg atggggcatc tctttcgcaa attcttcagg aggatctcaa
    14701 tcaattgagt cagttaattt ccaaagtatt gatcctagtg taggtactca aactattgaa
    14761 agcaataaaa gcggacgttt ctctttggat accgatcgta atttaactgg tacaagtact
    14821 ggtgctttac gtatcaattt ccatttctaa gctcgttaca atactttaca aaactcccgt
    14881 taatcgaaag gttaacggga gttttctttt gggaatattt ctccttaaaa tcatcgtaaa
    14941 tcgtctgcat tcaaacccta tatttgccaa ctaacgctaa ctaatatgtc atccatcatc
    15001 ccttccgcat tttttgagcc tagtaatttg caggctttgc gcaatcagtt tgatgccgga
    15061 aaaccatttc gacatctggt tattgacaat ttccttgatc ccgaatttgc caacaccctt
    15121 tatgagaatt tccctggatt tgatttaatg tcaaggaatt atcatggttt aaatgaacat
    15181 aaatctgaag gttcgggctt tgaaaatttt catcctgcct ttaatcaact tcgtgccgaa
    15241 ttaaacaaac cggaatttta caaaacactc agcaccatta ccggtatcga taatttatat
    15301 tcagttgaag atgctctagg aatgggtgtt catcaaggtg gaaaagattc atacctcgat
    15361 gtacatatcg atttcaatat tcatactgct cgtaatattc atcgtcgctg taatctactc
    15421 atcttcctga ataaaaactg gcaagaaagc tatggcggac atgtagaact ttggaataaa
    15481 gacgtatcca aattggagca aaaagtatta ccaattttaa accgttgtgt gattttcgaa
    15541 acaaacgaaa tctcctatca cggttacagt aaaatcaacg tccctgaagg cgaaacgcgt
    15601 aaatcgtttt attcatattt ctataccgat ttacgcgaag acgcagctcg tttccacgac
    15661 accgttttca aagctcgtcc cgaagaaggc atcatcaaaa aagtaaaaac cgacgtgaaa
    15721 gaaacgttga agaataccat caaacgtaac ctcaaaaaag taggaatcaa tatctgatga
    15781 aacaggaagc aatccagtac acccaataca ccgaatatcc ttgtcactta agtcgactcg
    15841 agtggattta cgatacaatt gatcagggca gaaaacccag ttcaaaagtt caagttcttg
    15901 atgttggtgc cggaacaggt aatgtcacca ttccactagg cacaatcgaa aattccgata
    15961 ttcttggcat cgatattcat caaccaacac tggatattgc tattgaagcc aataagcttc
    16021 caaatgttca tttcaagttt gaataccttc aggatagccc tttagctgga aaagaataca
    16081 ttattctaac tgaagtttta gagcatattg gaccgtatcc cgagattcta gattatttag
    16141 ggaagaactc cgataagaat atgaagctac tcatcactgt tcccaatggt ttcggaccgt
    16201 ttgaaattgc gatgcaacct ttgtatttca tgcggaaaat cgggttgaat aatttcatcc
    16261 ttaaagttaa acggatactg ggtaaaaaag aaccctattc tcaaaactac gaaacgcctc
    16321 acgtgaattt tttcacggtt gggcgcatgc gtagagaatt gaaacagtac ggtttgaaaa
    16381 ttactgaagt aaagaaagcc tatgttttcg ctccaataat cgaaacttat cttcccttca
    16441 ttcctttgaa aggaattgct cgtttggata ataaattagc ttccattttg cctgcattca
    16501 tggcttctgg cttttatttt aaaatagaac acgagtaaat tctgactttt tttgtcattt
    16561 cagaacaaaa tgcttctctt attttaatta ggagaagcat ttattttttt atttaaaatc
    16621 atcagatttc aataaagtcc cacagaatac tggcaattga gagttttaac aattaacgga
    16681 ttaatttagt ggagcactga tttcttacac aaactttttg agttgatttc aaatcgaatt
    16741 tggaggaagt gcaattcaca actaacttgt accaatcttc cactgaaaca tctggaatta
    16801 agtgcaagca aaacaatcca tctttttctt tttactcatc ctgctacttt ctgcttcttg
    16861 caagaaagca agttctgatt tgtataccga taatgatcct ccagcttttt cggcagttcc
    16921 agctgtaaaa gtccggaatt ttgttaatcg tgcatttatc gatttaattg gtcgggaacc
    16981 taccgatgcc gaaatggaag caaataccgc cgccattcaa tcgggaaatt cgaacatgga
    17041 agctcgcgaa gctttgattg ataaattgat gtccgacaca actttccgtg atggagatac
    17101 ttcctacaca ttagcctatc acaaacgaat ctatgattta gggaaaattc gttttatgga
    17161 tggtgtttcc gaagcacaac tcattcaaca agcgggcatt tatcgaagca acgccatctc
    17221 cgattcatta agcggaaatg ttcctggtta cgaagaagat atgatgcgat ataatcgaat
    17281 gaaagatgta ctgctttcag ctcgacgata tatgcaaggc gaaatcacca ttcagcaaca
    17341 gtttgctgcc atgattaaca acgctattta cgacatcatt aacatgaact ccttcaattt
    17401 cgtaaatgcc tgtttcaacg atttgtttta tcgttttcct agtaatgctg aatttcaaat
    17461 cgcctacagc atggtagaag acaatcaacc gggcgtgcta ttcaatgcta ccgctcaaaa
    17521 taaagatgag tacgtaacta tcctcactca aagtcaagaa tgccgacaag gaatcatcat
    17581 ttggtcatat caaaccatcc ttgctcgtaa tcctgtatca gccgaaatct atgatgaaat
    17641 gctcttattt ggtcaagacg gcgatttcaa agctctacaa aaacgtttac taaaaagcgc
    17701 ggaatatgcc aatttctgat ttcaagcccc gttcgctttc tctcgtattg ctgatttcga
    17761 ttttgcagtt cggactttca tcctgcaaaa aagaaattga acccgatcaa tatgaattga
    17821 atcaagtagt gctttatcct tctattagcg ctaaaaacaa actcaaatca caggagcaat
    17881 tcgtgtctat tctttacacg aatttgtttc aacaagcttt atcgggtaac aaactagtcg
    17941 agatcatcga ctgcctcgaa tcaattggtg ataaagaact tggaagagaa gtactcattt
    18001 ccaacttcat gaacgatcct gctgttcaac ttccatccga ttcaaccatg cgggcaaatc
    18061 ccgaagcgtt tgtggcagaa acctacgaac gtttttttgt acgcgagcct tctcaagcag
    18121 aactcacttg gtttagcaac tttatcaatg cccatcctca attgagtgcc gaaatgatct
    18181 atttctcctt cgcactttct aacgaatatt tgtattacta gctgcccatg aaccgtcgtc
    18241 gatttatccg ggctgccgga ctcacaaccg ctggagcatt tgctgctcct tacattcttc
    18301 catcagggcg tttgtttgct tcttctggac aaccacttgc tggacatgta gtgtatgtgc
    18361 tttttgctgg cggtgttcgt caacaagaat cagttttgca acgctatttg gatgattctc
    18421 aaggattacc ttgggaagga aacatcatgt acaacatgct taatggcgat gctccacaga
    18481 ataagattgt gtatggaact gatcaaggtg gcggggcaga aggcggtact cccattcctc
    18541 gaattctcaa tcaaacgctt caaacgcaag gattactttt caaagaagtt agcagccaat
    18601 cgaacggaca ttatggtggc ctaaacaacc tccttcaagg aagtacagtc accactcaag
    18661 gattgaagca aaagcccgta aatcctacca ttttcgagta tttacgtcgt catggaggat
    18721 ataaagcaac cgatgcttgg tttgtgggaa atggtattgg gaattctatt ccattgctta
    18781 attacagcaa tcatcccgat tatggatcta agtatggagc aaacttcttt gcaccgtcgg
    18841 taacctttgg aaatctaggg tttgagcatt tgtcaaatgc taaagtgtat catcccgaaa
    18901 acgagctcga tcccatgtac caaatgaagt actttctcga tcaaagcttt agtagtgtag
    18961 ggcagggggg cgattcctta ggaaataccg aggaagaaaa gcaacgcatc aaacagttca
    19021 tgcgcgatac atacgagcgt gtcacacaag gattgattgc atttccgcca gttacggata
    19081 acagcgattc gttaaacata ggatttgcct gcgaagtgat gcgcgaattt caaccggcaa
    19141 ttacggttgt caacctaagt gatgtagatg gttgccatag cgacttcacc ggctatctaa
    19201 gagctttaca ccgcgctgat catgccgttg gacatttgtg gaattacatt caaacgcaaa
    19261 ttccgggaat ggcaaacgac acagttatta ttgcttctcc tgaatgtggt agaaatctgc
    19321 aacataatcc aatcaaggat gtgaacgatt ggtacgcata tgaccacagc gattttaatt
    19381 cttcacgtgt gtttacgctc atggctggac catcggttcc ttcgaatttg gttttaggtt
    19441 cagagaataa ctcgattgga tttacttctg ataatgttcc aacaatagcc gaaattcttg
    19501 gggtgaaaga tgaagtgatg aatgctggat ttcttcaatc gggcgctcga tcattatttg
    19561 aacggatatg agaaaggcgc tgattctatt tctctgcatc ggattcttct cctgtaaaaa
    19621 ggaacaagaa gaaaatccct atgacttaat tcaaaatccg aacgatactt ctggaacgca
    19681 tcataccgtt ccagacccta attcgctagc cggaattcac cagaacatat ttgaacctac
    19741 atgtgctaac tcgggttgtc atgatggaac ttttgaaccc gattttcgaa ctcccgaaag
    19801 cacttacaac accttagttc ttcgcccaat tatcaaaaac gatccggcag gaacctatac
    19861 ctatcgtgtg gttccaggcg attatctaaa tagtgttttg taccaaagat taatcatcga
    19921 tattgatggt caatcaggca ttatgccttt agttctggaa ccttcatccg actggaattc
    19981 gaagcaagct cagtacatcg agaatgtcaa aaattggata caaaatggag caaaggacat
    20041 tttcgggaat tcagctgcaa actttaatct tcctcctcag ttattaggga tatttatttc
    20101 cgaaacgggc agcacgcaag cttttccacg aaatgcaaca agtggttcca ttcaaattcc
    20161 aattggaact tcttcgattg atatttggtt agctattgag gatgatcaaa ctaccattga
    20221 tcaattgtct tatttgaaag caaaagtggg tgcttctatg aacgattttt cagctgtttc
    20281 ggagcaaaat cttcagttag taaacgaagt gatggaaaca ggatattcag gaaatcaagt
    20341 aagcttccga tttaaattca cacttccgtt atctggtcta acaccggttc aaccgtggtt
    20401 tatacgagcc tacattcaag atgcaacggc ctcaccttcc gaattacctt ctactggaag
    20461 tgccgaatac atcaaacatt tttactcttt tcaattaggt gaatgatgag aaatatgctc
    20521 ttcctattct tccttgttgt tttgtggtca tcctgtaccg aaaaggaacg tgtgcctgca
    20581 ccagcacctc ctgcaattgg aatcatttct atcacacccg attcaattct acaattccaa
    20641 gattctgcat ttgttaccat tcaatatgaa gatgtaaatg gcgatgtggg aagttctgat
    20701 ccggatgcaa aagacttaga agtgaaagat tcccgattgg ctcaacccga tttatattcc
    20761 atccaacctt tgactcccga tgctattgag ttattcattc acgggcaaat aaaaattaag
    20821 cttcccacct tatttatact cggaaatgga gattacgaaa cagttacttt ctccatacgt
    20881 ttgcgcgatc aaagtggaga atggagtcaa actgttacta ccgatccaat tgtgattaaa
    20941 cgtcaatgag ttttattcgg aaatatggat ttctcttttt gctgctcgta tgcggattca
    21001 atccagtatg tgctcagttg gagtttaaca tgtccgacac gctcgtaacc gaatgcaaag
    21061 ggattttata tgatgatgga ggcgatggag tgaattactt tcacaatgca aacgaaacat
    21121 tcaccatttg tttagatgca ccgggaacct taaatctcgc atttgatttt ttatgcattg
    21181 aattgggttc cgattctctc atgtttcatg ctggaagctc gattcttgat cctcaaattg
    21241 gtccagcgta ttcaggaaca actccgcctc ctgttcacac gataaattcg ggatgtatca
    21301 ccttacattt tgtttccgat gctaatgtag cttgtaccgg ttgggaagct cattggacaa
    21361 cccaaattgt tcctcccgtt cctcctctaa ttgctgcgat tattcccgtt ccggcatgtt
    21421 cgagtagtac agccatcatc aacctaagta aaaatattgc ctgcgattcg gtctatgctg
    21481 ctgcatttga actcattgga ccattaaatg ttcctattct ttctgcttcg ccaatatctt
    21541 gtacaaacga tagcacgcag caagttcaaa tcacttttgc atccggaatt gacctaggcg
    21601 gaaattatca aatcgaattt accactcatt attgggatgc ttgcgatagc ctctggacat
    21661 tcacaacgag cgataatttc agcgttttcg attgcccgat cgtggttacc ttaactcctc
    21721 aaaacgatag tatttgtttt ggagattgta ccatcatcga tgtgttagtt accggtggtg
    21781 atggaaatta cacctattcc tggaacaccg gaatgagcta cgccggacct gctcaaacag
    21841 tttgtccgct caatgatatt acctacacac ttaccgttga cgatacttct ccagcactag
    21901 catcatcggg ttctacaagc attactgttt ttcaaccagc tactgttccg gcaccatcgt
    21961 ctcattgtca gtcggatgca ccattcgatt taagtgcaag tccaacaggt ggttggtgga
    22021 ttggctctgg aattaccgat tcgctagcag gtacattcaa tggtgattct gctgttttag
    22081 gattaaatca accgggatat tatttaccaa ttacacccag tttcggttgc acaaccttga
    22141 ctaatattac tatcatacct atcaatgcag gtattgccca agctgcttgt cccggttcgg
    22201 caccgtttca aattattggt tttagtccta ttggtggaac ttggtcgggg ctatctgttt
    22261 cgccttctgg tgttttcgat cctgttgctg caggtgttta cacagttacc tattcggtga
    22321 atggttgttc cgaagacctg acaatttatg tggatgacat ttctaatctg cctgtattaa
    22381 ccgatacgat ttgtcaaagt gcagcttcta tattgtatgc attaaatcca cctggcggaa
    22441 ggtggacagg cgaaggaatt attgattcac ttactggttt atttgatccg ggagaaaccg
    22501 atgctggact tattcctata acctataacc tcaatggttg ctcacaaacc atagaagtat
    22561 atgtgaaaga aatttttgct ggttgggatc aaactgcctg tccttcccaa gacacttttt
    22621 tactagccga tttttctccc attggcggaa cttggagtgg aaccggaatc gtcgattctt
    22681 tacttgggat ttttgaaccg ggtacaaata ctaatcaaaa tttcacaacc gatctcattt
    22741 actcccatcc aaacggttgt accgatacaa ctaccatcta tgtagtgtat tcaagaatat
    22801 atcgcgatac cattgtgttt tgctcgggtg atcaaggtat tatactcgat catttcagta
    22861 tggaaacaag tccatggtcg ggcaattgga gtggaaatgg cgttattcct ggagctgatc
    22921 aagattcctc attcttcgat cctaacattg ctggaacagg cgttcatctc ttaacattcg
    22981 acataaacac ctgtgccgat actgcataca tgattgtgca aaaagcattt cttcccgata
    23041 ttccattaac ctgcgaacaa gctcctgctt tccaaattac agttcccgaa tatgctttag
    23101 gtggtacttt ttcgggtgat ggaattactg attccattgg aatgttttca cctagcgatg
    23161 caggaactgg tttacatgaa atctattatg aaagtccaca aggttgctta gacacgctca
    23221 ttgctgaaat agattcctat caagaagcta gcatcgacgg acctgctgga gttttatgtt
    23281 ataccgattc gctctattcc gttaccattc taccgcttga tgcgttatat tatggaacgg
    23341 gttcggtctt gccgggtagt tttaatccaa tcatttcggg cgaaggcgat cattggctat
    23401 atgccgaaac aggacaagga tactgtcgca gcatcgactc ggtcatggta agtgttggtc
    23461 cagcaatcgg ctattcctta gtcgttagta ccgattcgat ctgttatggt gattatgttt
    23521 cgatgcaggc gaatgctttt ggaggaaacg gaaatctcat cacttataca tggaatcaag
    23581 gtttgcaacc attacagcaa caagtagtta gtccacttgt ttcaactcaa tataccttag
    23641 agatttccga tggatgttct cgattgcgcg atacggttga tattactgta agtgatccaa
    23701 ttacatattc aatcagcaca agcgattcac tttgttatgg tcgtcccgga tttgctcaaa
    23761 ttgatggagg tcattcaagt tcctatcaag tagtttggga taatcagctt tacattacag
    23821 gtgaacaatt gcctggttta gcatccaata cctattcagt ttctattcga gatacagtat
    23881 cgggttgttc ggttgatact ttgattacca ttcctggttt tccattggta aaagctcagt
    23941 tctcggttaa tccagaatac gattgtattc cagaggaaat aaaagacatc aatttcatcg
    24001 atttaagttc aggtgctttt caaggatctt ggaatttcgg cgatggtact gtaattccgt
    24061 atgtctcagg agaaaatcca acacattcgt atcctattta tggtgaatat gtagttggtt
    24121 tagaagtcgc tgacagcaat ggttgcagcg ataaaactga ccgtacaata tgcttgaaag
    24181 agccttttaa agtttatttg cccaatgctt ttaccatcaa tgaagatgga ctaaatgaag
    24241 tatttctagc taagggaact ggaattctaa cttttcacat gtgggtttat gaacgaagtg
    24301 gaaaagtgtt gttcgaatcg gatgcgttag ataaaggttg ggatggtaaa ttgaatggac
    24361 gaattgtacc aatgggtgtt tatgggtgga tagttgaagt tcaatggacc gataaacaat
    24421 ggtttacaac tgcaggaacg gtaaccttaa ttcgttaatt gaaggctttt cttttttaga
    24481 ttagagccat gctacctgct caacactttc cttttattgg caatcaatac cgaacccaag
    24541 tttctggatt ggatttgctg gtttatcata aacataccaa cgaattactt gcacgtattc
    24601 ctcaaattgg tttaaatcaa gtggaagaag caatcgcctc taccatcagt ggttttcagg
    24661 aaatgcgcaa actcgatatt tccgaacgtg ccgccattct tcaaaagatt cgtgatttat
    24721 tggatgaacg taaagaagaa ttcatccaat tgatcgttgc cgaagcagga aaacctatat
    24781 cctttgctcg acaagaacta gatcgctgtt tactgaccat cgataccgga attcgggaag
    24841 tttggtcttt gaatggagaa caaatcccaa tgaatgcagg aagcggaaaa ggtaaaattg
    24901 cattcactca acagtttcca accggaccag ttttaggaat ttcacctttt aactttccct
    24961 taaatttagc attacataaa attgttcctg cgctagctgt agggacgagc attcttctaa
    25021 aagctccacc tcaagctcct ttatcggttt taggtttagc cggtttgata gccgaagctg
    25081 gagcacctgc tggagctgta aatgtgattg tttgcgatat tccagttgcc gaaaagctag
    25141 taagtgattc ccgttttagt gttctatcat ttactggaag cgctcgtgtt gggtggcact
    25201 tgaagcaaat tgcaggaaat aaaaaagtac tactcgaatt gggcggaaat gctcccgtta
    25261 ttgtggatga aacggctgat ttagccgaag ctgcccgaca aattgcccgt ggtgcatttt
    25321 tatttgccgg acaaatctgc atttcaactc agcgaatcta tgtggttgaa tcggtggcag
    25381 atgagtttcg tgatctgtta ctttccgaaa cttcgcgtat ccgtagtggt gatccttccg
    25441 atgaatacac catcaacgga ccaatcatcg atgctaatac gatacagcga attcaggaat
    25501 ggcttcaaga agcaaatcgt tcgggagcag aaattcttgc gggtggaaat ttgattgatg
    25561 ccgaatgccg gattttttct cctacgattc taactcgtac aactcccgaa atgaaactgg
    25621 tgaaagaaga agttttcggt ccattagcca ttttcgaaat cattcgaaat ctggaacaag
    25681 gcgtcgattt agctaatgca agcgaatatg gtttgcaatg cggagttttt accaaccact
    25741 tggaacgaat aaaatacgcc tatcaaaagt tggagtatgg agctgttatc atcaatagtg
    25801 ctccgggttt tagggttgat caaatgcctt atggcggaat caaatctagc ggcttagggc
    25861 gggaaggaat ccgctacgct atggaagctt atacagaaaa gaaactactc gtattctaat
    25921 cagtccttgg ttattgtatc agcctttcgc atttttgata gaatcgtcaa ctaaattctt
    25981 taactagaat ctttacattg ttccacctaa attcaaaatt caatcatcca tatgagtttt
    26041 caccgcatcc ggacgctgga agaatatcag catgcatacg aagagagtgt taagaatccc
    26101 gaaggttttt gggctgatat tgccagccga ttgacttgga aaaagaaatg ggataccgtt
    26161 ttagaatgga acttcgatga accatctgtg aaatggtttt ctggcggaaa gctcaacatc
    26221 accgaaaatt gcctcgaccg tcacctcgat acagaaggaa ataaaatcgc cctcatctgg
    26281 gagccaaacg atccaaaaga acgtgctaag aaattcaagt atcgtgaatt atacgtttta
    26341 gtttgtcggt ttgctaacgt acttaagcgc aatggcgtgc gaaaaggaga ccgagtttgc
    26401 ttgtatatgc ccatgatacc ggaactcgca attgctacac ttgcttgtgc tcgaattggt
    26461 gcagtgcatt cggtagtttt tgctggtttt tctgctcaat cgatagctga tcgcattaac
    26521 gattcggcct gttctgttgt tatcacagcc gatggaatga gtcgtggtcc taagcaatta
    26581 cctttaaaac gtgttgttga cgaagcgctt gaacaatgtg cttctgtgaa tcgggttatt
    26641 gttcataatc aactgaattg ggatcccgaa atgattgaag gtcgcgatgt gtggtggcat
    26701 gaagaaatgg aaatggtcga tagcgattgt ccagccgaaa gcatggattc ggaggatatg
    26761 ctgtttatcc tatacacatc aggctctaca ggtaaaccaa aaggtgtggt tcacacgtgt
    26821 ggtggctaca tgacctatat cgactataca ttccgaaatg tttttcaggt agaaagtaat
    26881 gatgtgtttt ggtgcacagc cgatattggt tgggtaaccg gacattcgta tttgctatat
    26941 ggaccattaa tgtcgggtac aactaccttg atgtttgaag gtattccaac ctatccagat
    27001 gcgggtagat tttggagtgt aattgataaa catagcgtga gtgtgtttta cacggctcca
    27061 acagcaattc gatccttaat ggcagcgggt gtcgatcacg ttcttgctta tagtttgaat
    27121 tccttacgag tattaggttc tgtgggagaa cccattaacg aagaagcttg gcaatggtat
    27181 aacgtgcacg tgggcaagga aaaatgtcca attgtagata cttggtggca aaccgaaaca
    27241 ggaggaatga tgattagtgc gatggctgga atcactccat ctaaaccaag tttcgcagga
    27301 tttccacttc ctggaattca acctgtatta ttgaatgccg aaggacaaga aattcaaggc
    27361 aatggtgaag aaggctattt ggcgattaag ttcccttggc caggaatgat acgaacgacg
    27421 tatggcgatc atgaacgttg tcgagttacg tatttctcaa cctataagaa ctactatttt
    27481 acgggtgatg gagcgcgtcg cgacgaagaa ggcatgtatc gaattattgg tcgggtagat
    27541 gatgtaatca atgtatctgg gcatcgtttt ggaacggcag aaatagaaaa cgcgatcaac
    27601 caaaacaagc atgtaatcga aagtgctgta gtaggatttc cgcatgatat aaaagggcag
    27661 gggatatatg cttttgtcat ttgcgataaa ctacccgatc atcctgttca attaagagaa
    27721 gagatttacg aaacggtaag tgagcaaatt ggtcgaattg caaaacccga taagattcag
    27781 tttgtaagcg gtttacctaa aacacgttcg ggtaaaatca tgcgtcgaat acttcgaaaa
    27841 gtggcagaga atgaaattgg taatttgggt gatgtaacta cattgctaga tcctttaatt
    27901 gtggatgaaa tcattgctgg caaattgtaa ttactaacat attaattcaa atggctgata
    27961 aagagttatt tgagaaagtg tgtttgtgta attatatttt cgaattttcg ttttttgatt
    28021 tgatagttta ataattttga cctgttctaa tggatggtag tagaaacaga tttcgaactg
    28081 caggcatgaa aacatgttga agaaattcgc aacctaacct ctgtctcact cagagaattt
    28141 ccattggttg cccgacccat atcgggaaga tggtttgtga gaaccccgta tgttcattcg
    28201 tgcggggtta ttttttgtct aattctagaa gtcaagacaa gccttgtgtt tttatatctt
    28261 gccgtttcct aaaacattca tcatgaagaa caaggttgtt gcagcagccc tgatggtcat
    28321 tttatgcaat acagttggat ggtctcaagt gaaaaacgat actatttcac ctactgttaa
    28381 ggatctaact tttactggta ctcgtataaa tccgtacgat gcagaaacag atactagcgg
    28441 taaaattgaa tttagtggat atgtagatgc ttattatgca tgttataccg atactgcttc
    28501 ggttagtaca tttcaaaaat ttccaacaat agcgccgcga agtaattcat ttggtttgaa
    28561 tatagctcaa gctagtgcac gttattcaag tcctcgattt cgtggagtga taacagtttt
    28621 tgcaggagat gtagctagaa gtgcttggtc gcctgaattc tccttcattc aagaagccaa
    28681 tgctggattt cgtattggaa agaaggtctg gattgatgca ggattttttc gaactcattt
    28741 aggtttggaa tctattcagc ctcgcgaaaa tattacctta agcattgcta ctactactta
    28801 ttttgaacct tattacttaa gtggagcaaa gcttacttgg gagttaaatt caaaactaac
    28861 tcttcaagca aatgtgttta acagcttcaa tggttttgtg gaaaataaca gaaataaagc
    28921 acttggtttt tccatggcat ggcaacctat aaaaggattg agttttactt acggaaatat
    28981 actgtgtgaa gaaactcctg gagaattata tggtaagaag acacgcttct acaataactt
    29041 atttgctgtg tataaacggc gacgcatcac tcttggtgca gaagtaaatt atgggacaca
    29101 aaaccatact ggcattaatg attcgcaaca tttggcctat atgtttagct ctttgctagc
    29161 ctttaaatat cgacttacac ctcaattagc tttgtataca cgaggtgaat attttgccga
    29221 tccagatgaa atgttaactg gaccagttca aaacgaatat catcaattag ttggattacg
    29281 tgttggtggt atcacacaag gaatagaata caaaccaatt cccaattctt acctaagatt
    29341 ggaaggtcgt tggttagatg cacgaaaaga tgaacatatc tttttattac gaaacgcacc
    29401 gtctcacaat cggtacgaat tattaggtgg actgggactt tggttctaaa aaaaatgcgg
    29461 attccacata atgaaatccg cataatcagc tagttatata ttgtttattc ttttaccaga
    29521 actccatctt ttacttccac atccatcaag taaccattgc aaaaaccttt gaacgttgct
    29581 tgttcacccg atttaacagc atcggcatct tttaaatgac gtgtgtctaa tgttacacgt
    29641 accgctccca tcatggcatc aggaaatgta agcataacac gagaaatggt gtccgacttc
    29701 tctatatcgc ccacagtacc agtaacttca acaacttttc caacataagt tgagtttgct
    29761 tgttgctcat tcgattcaaa agccttgtaa agtacatccg ctgtaacctt tactccttcc
    29821 atttcggaag catcagcatg aggtttattc cacatacgat atgcaacaat acctccaatt
    29881 gctccgccta caagcaatac agctaaaatt atcttgatcg attttttcat tgagttgagt
    29941 ttgaatgagc aaatgtactg ttcacttttt aatttatacc aacataatag ttaactgaat
    30001 ttatatgtaa gtgtaaataa tttttcgatt ttaattaatc ggggttactt acttg
//
LOCUS       DAOOQW010000007        29318 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_1844_length_29318_cov_5.601750, whole genome shotgun sequence.
ACCESSION   DAOOQW010000007 DAOOQW010000000
VERSION     DAOOQW010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 29318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29318
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1844_length_29318_cov_5.601750"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            89..565
                     /locus_tag="PK281_01110"
     CDS             89..565
                     /locus_tag="PK281_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002665596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HOY47965.1"
                     /translation="MAIETEFKFLVHKDLWNQVIPNKSLDIRQGYLYSDENKTVRVRT
                     KGEKGYLTIKGKTYGASRPEYEYEIPLDEAIELLNLFCPERIEKTRHEILFAGNKWEV
                     DVFQGANEGLIVAELEVESETDAFEKPSWIGENVTDDSRYANSNLIKNPFVKWKST"
     gene            616..2256
                     /locus_tag="PK281_01115"
     CDS             616..2256
                     /locus_tag="PK281_01115"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOY47966.1"
                     /translation="MKQSIINRFNKAQYYLLLIAFIFLFKTEHSKAQKHVILIIADDL
                     GVDYCGFQEGFADTISLPNLRSLVSRGIVFNNAMSNPLCSPTRAGIYTGRYSFRTGVG
                     DVVTSPTASVALDTSEMCISKILGLADSAIKKAQFGKWHLQAGTPANLLNPNYMGYDK
                     YAGNFSGALTSFYDWTKITDGISSTSNTYATTEVTNDAIAWIQEQNALSSYFVWLGYN
                     APHTPYHLPPAELITTTGLSGTVNDINTHPIPYFKASMEALDHELGRLFDSLRVLNEL
                     DNTDFIFIGDNGNGLRVSQIADSSRAKGTIYQYGVHVPMIISGPSVISPGRASDALVN
                     TVDLFATINELMGVNNWNQFISQERPVDSKSIVPILRNEIDSIRPWSFTELFKVVSDS
                     ADGKAIRNKEYKLIRFDYGAEEFYKVNGDPMELTNLLASSLDSASQWNYYYLCNELNN
                     LVGSGPECPVITSLADDDHLHESTIYPNPADEYFNLISDHTKQAIILSISGHVCESIS
                     LKNGINKISVKSLHDGIYIVLDENGKAQKLIVNHSSEK"
     gene            2345..3190
                     /locus_tag="PK281_01120"
     CDS             2345..3190
                     /locus_tag="PK281_01120"
                     /inference="COORDINATES: protein motif:HMM:NF039776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY47967.1"
                     /translation="MKRNLLLIAAFVLAFQIQTIAQTTDPAPYCSAQFDDVDGTFDVP
                     DQIKSMSFGTLENITNAQCAYPHYVLYNNLTAPNLSIGSSFPLSITFEVHGGSGYGVW
                     IDYNQNSVFEPTEKVAGSTFDSFLEISDNTVVTGDITISPIAMAGQTRMRVRIVEDDM
                     YTAVNGAAISACNASASAEDIMDWGETEDYIVNIISTVGISPVEVSSSISVYPNPTQN
                     TLFVQSAKPYSYQIYNTTGAFIQQGNNTGGNSISIAELPNGIYFIRLIENNQITTKRF
                     VKAAN"
     gene            3364..4077
                     /locus_tag="PK281_01125"
     CDS             3364..4077
                     /locus_tag="PK281_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008238855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HOY47968.1"
                     /translation="MKVEVWSDIMCPFCYIGKRHYEAALKQFADSNRIEIVWKSFQLD
                     PTIPKNPENKNNVYKYLADRKGMSEAQSRKMHDNVVEMAKQAGLNYNFDHAVVANSFD
                     AHKMIQLAKTKGLGDAAEERLFRAYFIEGMDFGDAATLVKIGQEIGLQAAEIEAALVS
                     EEYAKKVNADVAEAAQLGIQGVPFFVFNRKYAVSGAQPTEAFLQTLQKSFAEWEKANP
                     KSAFEVLEGPVCKPDGKCE"
     gene            complement(4535..6313)
                     /locus_tag="PK281_01130"
     CDS             complement(4535..6313)
                     /locus_tag="PK281_01130"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY47969.1"
                     /translation="MNIKLQIKILQLIILRSILILFLGISSNAFSQSFYTNWAYPIGG
                     PSYDFGLNNCIDLNNNVYTLGVFSDSLVLGDGQTQFKSNGRTDFYIIKHDTTGAILWA
                     KSFGGTEYDGAGAVVVIEFQVVPFPCGVKTDSSGNVYVFGNYAGTVDFDPGADDYFLT
                     ADEFTYGFILKLNPEGDFVFAKSYESTYFQSFDVSESSIYLGGTLYQTVDFDQGPGEH
                     ILTNTGQDAFLLKLSNDGDFQNAMQLFSSGYAGVNQIEVNDENQAVYISGAYKSDLDL
                     DSSMISDPLQQLTSQANTDGFVAKLGVDLDVVEWVKKMNCSSDGILNELSINSSEDII
                     LSGRFKDTMTVILNGIVNQIFTQGLFDSFILKVNPEGHFEKILVIGDSNSNSISAQTL
                     DPQNRIYISGSLKSPLNITFPDETILIEQTGLRNSYVLIIDSDLNYINHFTAIGGNYI
                     CDIEDIGIGLSNEINLTGTFTDNPTMSYDPTSILATYQGDYDGIQIQVIFDFLENSTD
                     SEDRTGIKIYPNPVTDCINFDIKTTENLVSVQLLDATGRLILQQMFTSIGTQTLSLRG
                     LAKGIYYCRLSKDGIPIQTAKVVKVN"
     gene            complement(6799..9282)
                     /gene="hrpB"
                     /locus_tag="PK281_01135"
     CDS             complement(6799..9282)
                     /gene="hrpB"
                     /locus_tag="PK281_01135"
                     /inference="COORDINATES: protein motif:HMM:TIGR01970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HOY47970.1"
                     /translation="MPIDPIATGLPVADIIHEVQQQLQERTTLIVTAPPGAGKSTLLP
                     LTLLDAVWLNGQKILMLEPRRLAAKTIAQRMADLMGEEVGKTIGYRVRFENRVSANTQ
                     LEVLTEGILTRMLHDDNALEGVGLVIFDEFHERSIHADVALALCREAQLVLRPDLRIL
                     IMSATLNLPVLSGLLQAPVIESKGRQYPVEIRYTGTQDEMMLVELTARVVLKASQENE
                     GDLLVFLPGEGEIRSCAELLSKSMPGVAIHPLYGQLPPHEQFLAIMPNKQGRRKVVLA
                     TSIAETSLTIEGIRIVVDTGFGRVSRFDPSSGLSKLQTVKISKDAADQRAGRAGRLSA
                     GVCYRMWSMADQGRMAEHRIPEILDADLAPLVLDMANWGVKDISQLTWLSQPPKAAVA
                     SGTETLHQLTALDEGKITAHGKALHRLACHPRIAHMLLMAEGEAAQQLATDIAAILEE
                     RDPLGREAGVDIHLRVEALRRDRSRNSIGKKMYRISQIAESYRRMLSIPESNAPADSF
                     ETGLLLAYAYPERIASARPGNNAQFQLANGKLAMMGHQDDLAHQAWIAVAHMDMREGM
                     GKIFLAAPLNPRDLMPLVKEKEILTWDTRKGGIIATKDLRIGSIVLQSKPLRDPNPAL
                     IRKAIAKAVQSEGLSLLNFDESMTQLQNRISSLHSWNAEQNWPDVSTAALLENNEIWL
                     GDFYDGIKKPEDLQRISLTQALLQFLGWDKQQILEQLAPSKIAVPTGNNIRIEYLPNG
                     ATPILAVRLQELFGMSDTPRINQGKTAVVLHLLSPGYKPVQVTSDLRSFWNNTYFEVK
                     KELKRRYPKHAWPEDPWTAAPVAKGRSMK"
     gene            complement(9396..10718)
                     /locus_tag="PK281_01140"
     CDS             complement(9396..10718)
                     /locus_tag="PK281_01140"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY47971.1"
                     /translation="MKKIYLLCSFVALCSVAYSQINGSTFGVVRKNYYSYVIDPIDTN
                     FNYQQLDSASIRLGKIDHATGLVTSLGEEQMNEAVNLTGAALNPYDSTYIFMGASQML
                     VISLTTGEVLNRVTISNPIAESYFDNFRFNNSDSTLYGLARRYNAATSTGEVYLAKIN
                     TTTGVITQISPTSVAQGFALAGSAINPYEMVFYFSIGSNILGLDLYSGEVYSNTPIQN
                     TDGIIFDNFTYSCSDTSLYGLVRRNYYTIEIDSISGFESQYFDSATVRLGKIDIATGN
                     VSVISNASLLGGGYSLNAGATIDPSTDTYYFSNGDQLIGVDIHTGVIVSNPNFSFTDG
                     MYFDLMRNFGDCRNAERMRQSPVITAIPSKQSTSTFEVFPNPANDLVQLKLSPENHFI
                     SITDLSGKSILRMNSNAVNSLQIPVSDWAKGLYIVSVDGAHHTKLMLN"
     assembly_gap    10932..11031
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11032..12344
                     /locus_tag="PK281_01145"
     CDS             <11032..12344
                     /locus_tag="PK281_01145"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HOY47972.1"
                     /translation="FCLSMMFYFRFLIAFILLHSTIQAQGPVIIGYWQNWISSEAPYI
                     PLDQIDGRYDIVCVSFAVLTSPADMNMQFVPESVSQSTFIQQMNTIQAQGKKVLLSIG
                     GANTSISMPDQTSHDAFINSMNSLLDTYPFDGIDIDIEHGNSILASGIISNPTSVDCV
                     YLIDAITQIKAHYAATHSNTMMLTFAPETAYVQGGMSAYGGIWGGYLPILDALRDDIN
                     YIHVQLYNSGTVYGIDGEIYAQGTADFIVAMTEAMIQGFSTSGGEFAGFPEEKIVVGL
                     PACTAAAGGGFCSPVVVASAIDYLHGNGNAPGNYTLVHSQGYPQLGGMMTWSINWDAV
                     STCNASPYEYVSNYEQLFETILSTEENGKEIKPVLFPNPANTFIHISNLTDSDYQISD
                     ITGKLIKAGKIDSNSTIEIDSLNDGFYWIYVNQSWIKFVKQSSN"
     gene            complement(12363..13118)
                     /locus_tag="PK281_01150"
     CDS             complement(12363..13118)
                     /locus_tag="PK281_01150"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOY47973.1"
                     /translation="MIKAILIDDERPSREALQNKIVMHCQDVDLVCMCNSAQEGLDAI
                     IKYNPDLVFLDIEMPLMNGFSFLEQVIDRSFQVIFTTAYDHYAIQAIRFSAIDYLVKP
                     IDVDDLKAAVERVKSSLANQSQDKRLEFLLKNLQQGQTPKTLAITTGQGLLFLNFSDI
                     TYLAADGNYTYIHTLNQKILSSKTLREFEDILPSQLFFRIHHSYIIHLEHLKEYHRGE
                     GGEVVMRDGNHLPVARRRKDELLETIKRFSSKV"
     gene            complement(13125..16193)
                     /locus_tag="PK281_01155"
     CDS             complement(13125..16193)
                     /locus_tag="PK281_01155"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOY47974.1"
                     /translation="MQSIRQLICLISGIIIYLSTCQHARAQGFSNRMYAYSTRDGLPS
                     NVVSKVLTDKDGFTWVLTTNGLARFDGNRFNSILQNNNKKLSIQSGAIKDIILDEAGI
                     LWVVQGNQLSKHLNQGFDFESYHLPDSLISNQIISCYVDSRKSQAIIGAMGDFLLLDL
                     KTGKYRSTGWRQYVRNQIPLFKPLLQGNSIIPKSGDEYWLLATNGLFSYSTKTHLFNY
                     YPSDLIREFQGLSLYISAFNKLWMGSYNNGIVAFDIESKTWKSWLLPDSLLDSHHLQS
                     VYSLQVISPDKIIGISRNHVIVFNPINEQFTSLSINGLEGEPLPISPQALFIDKKANR
                     LWIACQHGVLRSLDEPVFDFYPIQIPGVNRFYTQFIDNEITDEFQALSIYTNELISLN
                     KQLLKTHSTQEIAQFPINFISAIRKDPKGNLWVCMRNEVIVRDAISAQWIRISLPESP
                     VNSKNRVFWDVVFDDNGFAYTCSWTDGVFQIGPDLKFKKIIPIPSTNTDCRYQAIKFD
                     TNFNRLWVATENQGVYCLNLSSNSSFWLKYQQNKPNSLPGYPIIDLELATDGSIWICT
                     ASNGLAHFSVEQNTMTHFNQQNGISSNFTYWCSFDKKGRMWVSCPSSINLFDPQTQSF
                     SSFGMESGWTDQEVFKPSRMRNGELLFGTMNGYFQIREKHFTTGIKYPEVYIRGVLLG
                     DSSMSITQKAMLNSDQNSITIQFGAIDYTLPDKLQFQYLINSDTAWKNTNERELNLIN
                     LPPGDYTIQIRAANIHHTWSKPVTWKFLIQTPFWKKGWFLILCISLIAVIAYLIIRRR
                     LIAIRSKAQLKQRIAETEMAALRAQMNPHFIFNCLNSIDNLIQQNDSLNAAKYLHRFA
                     RLIRDVLEHSRSTEIPFRKDWETIRIYLELEQLRQDGSINLTFEADAELLQGDYRVPP
                     LLIQPFIENALHHGLLHKMSGEKYLKIIAQVKGEELIYTIEDNGIGRDAAMKIKSAHS
                     KDRPSLGVQISSERIAFYTSRLGSSSIETIDLYQPDGSAAGTRVIVRLQT"
     gene            complement(16268..17791)
                     /locus_tag="PK281_01160"
     CDS             complement(16268..17791)
                     /locus_tag="PK281_01160"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY47975.1"
                     /translation="MNKIFFTGIIFSLFFSFQLSAQELDPTFGNSGKAFASFGFTSYY
                     FEEMEFQSSNKIIAVGDNENILNPIVVCRLKNDGSLDSTFQGTGILTFGFGFTYEFCD
                     ALVIDSQDRILLGGSSNGDLVLARLLPDGDFDTTFSTDGKVKLSFGLGNGSKAHKILI
                     QNDGKIVAIGEAYGGSNFNFAAARFHANGVVDSTFGVNGLITHDVNGFNDFGYDALLL
                     EDGKILIGGTAYNSDGNSAFAWIRLLEDGTIDTGFASNGKLILNINSNYNEVVEVMEL
                     HPNGKIISAGYTGGDFAVLCMNIDGVSDASFGSNGHITTDFDDYQDKAYALKIDAQGK
                     IILAGHGYDGSVNTFLDFAIARYTANGSLDNTFSSDGKLVMKMGTYSSGIADILIQDD
                     GKLLFGGQSFETQGGFAEFGFLQLDVSAITNIPTNDYSNFQLYPNPVVDKLTIRNDTS
                     DEDFELIQLYSITGQLLNEYKSQAQFVHLDVSTLNTGIYIVNVQTSKSSKTFKFIKQ"
     gene            complement(17812..19365)
                     /locus_tag="PK281_01165"
     CDS             complement(17812..19365)
                     /locus_tag="PK281_01165"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY47976.1"
                     /translation="MRYSVSILFFGFFFQAFSQAPDTLWTRCYGGASYENAKSVRPTA
                     DGGLIVIGSTTSYDGEVEGNHGSSDIWVVKLDESGEPEWTKTLGGTYQDEGFDIVQLP
                     DESYVLAGHTISQDGDVSMNHGLTYDAWIVKLSVDGDLVWDLSIGGSDNEELFGMTIS
                     GQNIIVVGNAYSTDGDISGNHGLSDVFIARISFFGAVENIVLLGGTSAETAYAVANSN
                     DQGFIIAGISNSDDGDVPTNLGQQDIWVIKTDYTGAMEWSKVFGGSAMDRPRGIIPTQ
                     DGGYLLLAESSSADGDLTSNSGFYDCWVAKLSQLGIVEWQQCFGGISFDGSFSGIQLS
                     DGSYVTANEIYAPESPGELTPHGGTDMVLKAFSADGTPTWESWYGGSGLERPYCLRRM
                     NDSSWVMVGETNSIDGDVTGNHGDDDFWVVKFGIESLPLGIKNSSSTHMKIYPQPATE
                     QITIEMDTELSDAHYTLFDVLGNKVQTGIVNLISTFSISVNSLPSGIYFLQVNTNTDQ
                     YSRSVQVLH"
     gene            complement(19463..21829)
                     /gene="pbpC"
                     /locus_tag="PK281_01170"
     CDS             complement(19463..21829)
                     /gene="pbpC"
                     /locus_tag="PK281_01170"
                     /inference="COORDINATES: protein motif:HMM:TIGR02073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HOY47977.1"
                     /translation="MKHFRFRFCPMKRVKRYLKFILPELFFVLLGMYTFCLPNQLFAD
                     PACTVLEDRNGNLLSARIAADGQWRFPYNSQVPYRFKQALIQFEDRDFYDHLGVSPKA
                     VGRAVWQNSQSMKVRSGGSTLTMQLARMSRKNSDRGIWDKLIESIWATRIELSYTKEE
                     ILSLYASNAPFGGNVVGLDAASWRYFGRPADKLSWAESATLAVLPNAPSLIFPGKNQH
                     RLLAKRNRLLNRLHTANFLDDTDLALALEEPLPQQPFPIPQLATQLLDRAIAEGHGAT
                     VIYSTVNGELQQQIQQVMMMHTMRQKSNDVHHMATLVLDLKTNEVISYVGNISDYSDG
                     HGMGDVVDVIRAPRSSGSILKPLLYASALDEGLITPISLLPDIPTHLSGFSPKNFNHT
                     YDGAVPAKDALARSLNIPMVRLLGDFGVEKFHQKLRRLGLSTINRPASHYGLALILGG
                     AEVSLWDLCNAYARIARELRADERKQSNDPHVASYIQGKSNAKNTLTSGFSPASAWLM
                     LDAMVEVNRPGEEAQWKDLGRGRKIAWKTGTSYGFRDAWSIGVTPRYVVGVWVGNASG
                     EGRPGLTGINCAAPVLFDIFDKLPYDGWFTKPVDKMKTLPICAQSGFRLTEYCSDSVF
                     LKLPESAMYSKACPYHQRIHLDAREPLRVNSSCADVGSMRHVNWFVLPPVMENYYAQH
                     TPTYAPLPAWKPGCEPEPEQNIALVYPRKNSRISVPIGLEGIPTATIFEANHRRSDAV
                     IYWHLDDEFIGSTRDIHQLQLQPMPGKHKLTLVDDQGNSISCGFEVLS"
     gene            22338..25355
                     /locus_tag="PK281_01175"
     CDS             22338..25355
                     /locus_tag="PK281_01175"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HOY47978.1"
                     /translation="MLRLFKILLLLLLFPLGIRAQQFHFRNYSLSEGLAQSQVYAIFQ
                     AKNGTIWMGTRGGGLSAFDGIRFQNYTTRDGLSDNYILSLAEDSLGKLWIGTNNGICT
                     FNGRIFQKETLPDSGTIVVESILLNRPNEILIGTQKGIYSYKSNKWTKFRADIPSIQE
                     NISCLYNDHSNRLWIGNNSGITCIDKKTVFHLGLKEGLPSEAIRCITEDAGGTIWVGT
                     YGSGIAWITKNGIRIINSTNVLPDPIVQSLFRDVQNRIWIGTQEAGAGFWNPSDSLFR
                     FINERDGLSNNHIRCIIDDYWGNIWLGTSGGGVSKYFGQQFTHFDQRSGLAGRNVYAI
                     AEDTSGVIWFSTSAGGLGRVEGNSIKDLSRADGFPTVKIKKLMTDLQNRLWIGTEGEG
                     LYMYSGQDFKHFGNENGLKGNWIRDIIQDLAGNIWIAKAGGGIAKLTEENTQFKFKQF
                     SLEEGLIEDRINALLQDKSGRIWFATTSTGCGFIQNDSIINFSTKDGLSSQKLRSLAL
                     APNGTIWIGTADRGLCAISMNGNKPVCQVISSKQGLTSDNIYLLNFDSDGKLWAGSEK
                     GLDRLTFSKEGKLEEIKHFGYSEGFIGIETSQNATLRDKQGKLWFGTINGLTRFNPAL
                     TDINKIAPILQLRDIRLFFKPLTETPFASFWETLSQDTSQILKLPFNQNHLSFDFFGV
                     NHRNPEQVKYQWKLVGLDESWTPPGFQHNITYSNLPPGDYTFLYRAANEDGIWSDAKA
                     IHFQIAIPIWKRWWFIGVCAGLVVILVWLYFRWRLRRIKRKAKEAQEKLKLETDVLKL
                     EQKALQLQMNPHFIFNALNSIQALIGTQDEKTARYYLSKFSRLMRQILESSREQIIPL
                     ELEISILENYLSIEQFSHGVKFTWEITYDESLSPSNTGILPMLIHPFVENAVIHGLVP
                     KGAAGHIHIQFSKVDEQLIVRVTDNGIGREKARERRSQQDQHHKSTALLVVQERLELL
                     EGKTHVQIIDLTNSDGTSSGTEIKVILPFTSLD"
     gene            25482..26219
                     /locus_tag="PK281_01180"
     CDS             25482..26219
                     /locus_tag="PK281_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOY47979.1"
                     /translation="MKLRALLIDDITEARNNLRRDIADWCPDIEIIGEADGVISGAKE
                     IRKQKPDLVFLDIQMQDGSGFDLLDILGEMKLHVIFTTASDAFAVKAFRYAAVDYLLK
                     PIDPDELKNAVAKINNQSNSNENLSLLKQQLSSKKQTDRIALHTLEKIHVVTVDEIIH
                     CESDDNYTRFYFQDKSKLLVTKTLKEFDELLSPLGFLRVHQSHLINLKQVKEFIKGEG
                     GYLIMKDGSNIPVSTRKKQEVIEKLID"
     gene            26244..26756
                     /locus_tag="PK281_01185"
     CDS             26244..26756
                     /locus_tag="PK281_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOY47980.1"
                     /translation="MINLQPTLENELLRLRPLMESDYEELYQCASDPLIWELHPNKNR
                     YKREVFDVYFQEAIEGKMAFAVIDLKTNKIIGSTRFYDYKPEQKSIAIGYTFLIRNYW
                     GGLYNKSMKGLLIDYAFQFSDEIILHIGANNFRSQKATEKLGAVKIAEELIPFPGEAN
                     ALNFVYQLKK"
     gene            26805..27320
                     /locus_tag="PK281_01190"
     CDS             26805..27320
                     /locus_tag="PK281_01190"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HOY47981.1"
                     /translation="MKQPLPSEYKPYFHRYISLVEGENFFEQYDKINQDFIQFLNSIP
                     ADKHAYRYAPGKWTIKQILRHLIDCDRVFAYRALVAARGDNSPLHSFDEDSYALAASE
                     ETCSLNYLIEEFKAVRFANRCMFEQLSEKQSQALANTEEHPMSVRAIAYILIGHVIHH
                     TSVIKERYLNE"
     gene            27429..27902
                     /locus_tag="PK281_01195"
     CDS             27429..27902
                     /locus_tag="PK281_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015025486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HOY47982.1"
                     /translation="MNFRNAIYSTFFLLFIAITSNAQESIKEYIPLDKFLYHQIDSMD
                     KVFFDAYNSCNIEKQESIYSDSIEFFHDKVGLINSKSEIIQGTRNNICGKVTRYIIPG
                     SIEVYPINNYGAVEIGFHRFYNNQEPDAESKPSKFIIIWKKEDVNWRITKVVSLH"
     gene            28165..29265
                     /locus_tag="PK281_01200"
     CDS             28165..29265
                     /locus_tag="PK281_01200"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HOY47983.1"
                     /translation="MKTVKFISADINQKQFAAEVRKNVLAYFKDNKISTKGDAGLYFR
                     TFLMIACYVVPFILIFSLTIPNWLAFVFCILMGVGIAGTGMCTMHDAVHGAYSNKKWV
                     NNLMGGTLYLLGSNVFNWKIQHNVLHHTYTNINGLDQDIESKGPIRLSDNAPLKKFHK
                     YQFIHAFLFYGLMTLAKLTKDFTQLATYNKAGITKNYKFNPTLEYIKMVVFKLAYISV
                     IIGLPILYTQFTWWQVILGFFIMHWTAGAILSTVFQMAHVVEGAEQPLPDKNGVVNCD
                     WTVHELNTTSDFAPKNKLLNWYVGGLNFQIEHHIFPNICHIHYPKIAPIVEATAKKYG
                     YQYNLKPSLWDAFKSHVIRLKELGQPLPAIAN"
BASE COUNT         9371 a         5512 c         5362 g         8973 t
ORIGIN      
        1 ccgaggctcc gaacacatac gggaaattgc tgcgaaggtt cttatttatc agcaatttac
       61 ttatcagtat taacccccat ttttcagtat ggcaattgaa acagaattca agtttttagt
      121 tcacaaagac ctttggaatc aggtcattcc taacaaaagc ctagacataa gacagggata
      181 tctttatagt gatgagaata aaacggtccg tgtgcggaca aaaggagaaa agggttattt
      241 gaccattaag ggaaaaacgt atggcgcctc tcgtcccgaa tatgagtatg aaattcccct
      301 tgacgaagca attgagctat taaacttatt ctgtcccgaa cgcatcgaaa aaacaagaca
      361 cgaaatatta tttgctggaa ataaatggga agtagatgtg tttcagggtg caaatgaagg
      421 attaattgtg gctgaattgg aagtagaatc ggaaactgat gcatttgaaa agcccagttg
      481 gattggagaa aatgtgacgg atgattctag atatgctaat tccaatttga taaaaaatcc
      541 tttcgttaaa tggaaatcta cttaacaaac taaaccattg tattaggaat gactcatagc
      601 gaataatttg aaaaaatgaa acaatcaatt ataaatcgat ttaataaagc ccaatattat
      661 ttgctattaa ttgcatttat ttttcttttc aaaactgaac attcaaaagc acagaagcat
      721 gtaatactta ttattgctga tgatctgggt gtagattatt gtggattcca agaaggtttt
      781 gctgatacta tttcgcttcc taatttacgt tcattagtca gtagaggaat tgtatttaat
      841 aatgctatgt cgaatcctct ttgctctcca acacgagcag gaatttatac aggacgttat
      901 agttttagaa cgggagttgg agatgttgtt acaagtccaa ctgcttctgt agcgctagat
      961 acaagtgaaa tgtgtatttc gaaaatatta ggacttgctg attcagctat aaaaaaagcg
     1021 cagtttggga aatggcattt acaagcaggt actccagcaa acttgcttaa tcccaattac
     1081 atgggttatg ataaatatgc tgggaatttt agtggagcat tgactagttt ttacgattgg
     1141 actaaaatta ctgatggaat ttcaagcact tcaaacacgt atgcaacaac agaagtaacg
     1201 aatgatgcca tcgcgtggat tcaggagcaa aatgcacttt cctcctattt tgtttggtta
     1261 ggttacaatg caccacatac accttatcat ttaccacctg ccgaattaat tacaacaaca
     1321 ggattaagtg gaacagtaaa cgatataaat actcatccaa tcccctactt caaagcatcc
     1381 atggaggcgc tcgatcatga attaggacgt ttgtttgatt cgcttcgagt actgaatgaa
     1441 ctggataata ccgattttat ctttattggt gataatggaa atggtttacg cgtttcacaa
     1501 attgctgatt cttctagagc aaaaggaacc atttatcaat acggtgttca tgttcctatg
     1561 attatttctg gaccatccgt aatttcgcct ggaagggcta gtgatgcatt ggtgaatacg
     1621 gttgatttat ttgcgacaat taatgaatta atgggagtaa ataattggaa tcaatttatt
     1681 tcacaggaac gaccggtaga tagcaaaagt attgtaccca ttttacgaaa tgaaattgat
     1741 tctattcgac cttggtcatt cacagaatta ttcaaagttg tgagtgattc tgccgatggt
     1801 aaagccatac gtaataagga gtataaactc attcgatttg attatggtgc tgaagaattt
     1861 tataaagtaa atggagatcc gatggaatta actaacctat tagcaagctc tctggattct
     1921 gcatcacaat ggaattatta ttatttgtgt aatgagttga ataacttagt tggttctggt
     1981 ccggaatgtc ctgttattac aagccttgca gatgatgatc atcttcacga atcaactatt
     2041 tatcctaatc ctgcagatga atattttaat ttaattagcg atcatactaa acaagcaatt
     2101 attttatcta tatcggggca cgtttgcgag agtatttcat taaaaaatgg aataaataaa
     2161 atctctgtta agtctctaca tgatgggatt tatatagttt tagatgaaaa tggaaaagct
     2221 caaaaactta tcgttaatca ttcatctgaa aaatgagaaa taaatacatc tgtatttaat
     2281 tgcctaaaat tagcaaatta aaatctagta tattcgcttt tcaactgcct caaaaactac
     2341 ccgcatgaaa agaaatctac tccttattgc agcatttgtc ttagcatttc aaatccaaac
     2401 aatagctcaa actaccgatc ctgctccata ttgcagtgct caattcgatg atgtagatgg
     2461 tacttttgat gtgcctgatc aaataaaaag catgtcattt ggaacgttag agaatatcac
     2521 aaatgcccaa tgtgcttatc cacattatgt gttatataat aatttaactg ctccaaattt
     2581 atcaattggt tcatcttttc ccttaagcat aacatttgaa gttcacggtg gatcaggata
     2641 cggtgtttgg attgattata accaaaattc cgtttttgaa ccgactgaaa aagttgctgg
     2701 ttctactttc gattcctttc tagaaatttc tgataataca gtggtaacag gagatattac
     2761 tatttctcct atagcaatgg ctggacaaac tcgaatgcgc gttcgtattg ttgaagatga
     2821 tatgtatact gcggttaatg gtgctgcaat ttcagcttgt aatgcaagtg cttcagctga
     2881 agatattatg gattggggcg aaacagaaga ttatatcgtg aatattattt ctaccgttgg
     2941 tatttcacca gtagaagttt catcttccat ttcggtttat ccaaatccaa ctcagaatac
     3001 attatttgtt cagtctgcta aaccatacag ctatcaaatt tataacacga ctggagcatt
     3061 cattcaacaa ggaaataata caggtggaaa ttccatttca attgctgaat taccaaatgg
     3121 aatttacttc attcgtttga ttgaaaacaa tcaaattact acaaaacgtt ttgttaaagc
     3181 tgctaattag actcaacata ttttataaaa gtttgtttca ttcaaagtaa ttttgattcc
     3241 ttataaaaca tgggatttaa aatccgattc ttcttttttt tactaatatt tagtcaagcc
     3301 tgttggggac aaatttcacc caatcaaacc acactatttc tcatgcaatc agatacactt
     3361 aaaatgaaag ttgaagtatg gagcgacatt atgtgtccgt tctgctatat cggaaaacgc
     3421 cattacgaag cagcattaaa gcagtttgcc gatagtaacc gcattgaaat tgtgtggaag
     3481 agttttcaat tggatccaac cattcctaaa aatccggaga ataaaaacaa cgtgtataaa
     3541 tacctcgccg accgcaaagg aatgagtgaa gctcaatcgc gcaaaatgca cgacaatgtg
     3601 gtagaaatgg ctaaacaagc tgggttaaat tataatttcg accatgcagt agttgccaat
     3661 tcgtttgatg cgcataaaat gattcaatta gctaaaacga aaggcttagg tgatgcagca
     3721 gaagaacgtt tattccgtgc ctattttatt gaaggaatgg atttcgggga cgcagctacg
     3781 ttggtgaaaa ttggacaaga aatcggtctc caagcagctg aaattgaagc agctttagta
     3841 agtgaagaat atgcaaagaa agtcaatgcc gatgtagctg aagcagcaca attaggcatt
     3901 caaggtgttc cgttttttgt ctttaaccgc aagtatgctg taagtggtgc acagccgaca
     3961 gaagcctttt tgcaaactct tcaaaaatcc tttgctgaat gggaaaaagc caatcctaaa
     4021 tctgcattcg aagtattgga aggtcctgtg tgtaaaccgg atggaaagtg tgagtgaaaa
     4081 ttcaattacg aattacgaat tacgaattac gaattacgaa ttatcaatta cgaattacga
     4141 attacgaatt aaaaatttga attcgttgaa aattgtgggc tttgtgaggt atccataaac
     4201 tactgaatat tgcttttgtc gtaattctca aagtatgatg ctctttcttg aactctattc
     4261 ctgcgccatg ggattgcagc cattaccgct tttgaaccga taggtgaaaa agtagtagag
     4321 gcgtaaagcc cggtgctttt gaagcaaagc ggaaaaagca ggcgccaaga aaagtacaac
     4381 gatatacgta ttacgtatta cgattttttc ggaggacggg cattcgtttt caaatgaaat
     4441 tcacgtaacg gaatctttct tcctagcagt ttttctcgta tgtcgtaaag tgaagtgatg
     4501 gtaaatcgta ttatccttaa gggacgcttg taattcaatt caccttcacc actttggcag
     4561 tttgtatcgg aatgccgtct ttactcaggc gacaataata aatacctttt gcaagtcctc
     4621 taagagaaag cgtttgtgtt ccaattgatg taaacatttg ttgtagaatc aaacgacctg
     4681 tcgcatctaa taattgaact gacactaggt tctcagtggt ttttatatcg aagttaatac
     4741 aatcagttac tgggtttgga taaattttga ttcctgttct atcttctgaa tcagtggagt
     4801 tttctagaaa atcaaaaatg acctgaattt gaattccatc ataatctcct tggtaggttg
     4861 ccagaatgga agttgggtcg taactcattg ttggattgtc ggtgaaggtg ccggttaaat
     4921 taatttcatt gctcaaacca atgcctatat cttcaatatc acagatataa tttccaccaa
     4981 ttgcagtaaa atgattaatg taatttaaat cactatcaat tattaaaaca taggaatttc
     5041 ttaaaccagt ttgctcaatt aaaatcgttt catcaggaaa tgtgatattt aatggagatt
     5101 ttaatgaccc tgaaatataa attctgtttt gcgggtctaa tgtttgagca gaaatcgagt
     5161 tggaattgga atccccaatt actaaaatct tttcgaaatg cccctctgga tttaccttca
     5221 aaataaaact gtcaaaaagc ccttgtgtaa aaatttgatt aacaattcca tttaaaataa
     5281 cagtcatagt atccttgaaa cggccagata aaatgatatc ttctgaggaa ttaattgaca
     5341 attcatttaa aataccatca ctcgaacagt tcattttttt aacccattca acaacatcta
     5401 aatcgacccc aagcttggcc acaaaaccat ctgtattcgc ttgtgaagtc aattgttgaa
     5461 gtgggtcaga aatcatgctt gaatctaaat ccaaatcact cttatatgct cccgaaatat
     5521 aaacggcctg attttcatcg ttcacttcaa tctgattaac accagcataa ccggaagaaa
     5581 acagctgcat agcattttga aaatcaccat catttgatag tttcaagagg aaggcatcct
     5641 gtccggtgtt ggttaaaatg tgttctccag gaccctgatc aaaatcaacg gtttgataaa
     5701 gtgtaccgcc caaatagata cttgattcag agacatcgaa agattggaag taggtgcttt
     5761 cataagattt tgcaaataca aaatctcctt ctggattgag ttttagtata aatccatatg
     5821 taaattcatc agcagttaga aaataatcat ccgcaccagg atcaaaatcc acagtaccgg
     5881 catagtttcc aaaaacatat acattacctg aggaatcggt ttttacgcca catggaaacg
     5941 gtacaacctg aaattcaatg acgacgactg cgccagctcc atcgtattcc gttccaccaa
     6001 atgattttgc ccaaagaata gcgccagtag tatcgtgttt aattatataa aaatcagttc
     6061 gtccattgct tttaaattgc gtttgtccgt cgccaaggac taatgaatcc gaaaaaacac
     6121 ctagtgtata tacattatta tttaagtcaa tgcagttatt tagtccaaaa tcatatgatg
     6181 gtccaccaat aggataagcc caattggtat aaaagctttg actaaatgca ttggacgaaa
     6241 ttcccaaaaa aagaatcaaa atactcctca aaataattaa ctgtaatatt ttaatttgaa
     6301 gtttaatatt catttcaatt tgattatgaa tttactaaag catttgtatc aaagtagaat
     6361 aagtatatag aaagagaatt tctaacaaca attactcatt taactaagta tagaattaca
     6421 gtgagagtaa atataataag attagtactt ccaactattt ttttcaacct actcaattca
     6481 cccaatttaa acttattcct gcgccatggg attgaagcca ttaccgcttt tgaaccgata
     6541 ggtgagaaag tagtagaggc gtaaagcccg gtgcttttga agcatagcgg aaaaagcagg
     6601 tgcccaagct cgtattacga tgtacgtatt acgtattacg attttaggcg gatatacact
     6661 cgtttcccag tgaaattcgc gaaacggaat ctttcttcct agcagttttt ctcgtatgtc
     6721 gtaaagcgaa gcgatcgtaa atcgaattat ccttaagtga cgctcgtaat tcgtaattga
     6781 acattcgtaa ttaatttatt atttcatact tctccctttt gccacaggtg ctgctgtcca
     6841 tggatcttcg ggccaggcgt gtttggggta acggcgtttc aactctttct tgacttcgaa
     6901 ataggtgttg ttccagaaac tgcgtaagtc gctggtgacc tgaacgggct tgtatccggg
     6961 cgataataaa tgtaggacta cagcggtttt tccttgattg atgcgcggtg tatcgctcat
     7021 accaaacaat tcctgcaaac gtaccgctaa tatgggtgtg gcgccattgg gtaagtattc
     7081 gatacggatg ttatttcccg ttggtacagc tattttgctt ggagctaatt gctccaggat
     7141 ttgctgtttg tcccaaccta gaaattgcaa aagcgcttgt gtgagtgaaa tacgttgcaa
     7201 gtcctcgggt ttcttaatgc cgtcgtaaaa gtcgcccagc catatttcat tgttttcgag
     7261 caaggcagca gtggatacat cgggccaatt ttgttcggcg ttccagctgt gtagactcga
     7321 aattcggttt tgcaactgcg tcatggattc gtcgaaattc aacaatgaca aaccttccga
     7381 ctgaacagct ttcgcaatgg cttttcgaat caacgcagga ttgggatctc tcagtggttt
     7441 cgattgcaat acgatgctcc ctatgcgcag gtcttttgtt gcaatgattc cacctttacg
     7501 cgtatcccaa gtgaggattt ctttttcctt cactaaaggc attaaatcgc gcggattgag
     7561 tggtgcggca agaaaaatct ttcccatgcc ttcgcgcata tccatgtgag ccacagcaat
     7621 ccatgcttga tgagctaaat cgtcttgatg acccatcatg gctagctttc cgttggccag
     7681 ctgaaattgt gcattatttc ccggacgagc agatgcaatt cgctccggat aagcatatgc
     7741 cagtaataat ccagtttcga atgaatcggc tggtgcattg ctttcgggaa tggaaagcat
     7801 acgacgatac gattctgcaa tttgtgaaat gcgatacatt ttcttcccaa tgctattgcg
     7861 tgaacgatcg cgacgaagcg cttctacacg aaggtgaata tccacgccag cttctcgtcc
     7921 caaaggatcg cgttcttcca aaatggcggc aatatcggtg gctaattgtt gcgctgcttc
     7981 accctctgcc atgagtaaca tgtgtgcaat tcgcgggtga caagccaaac gatgcaaggc
     8041 ttttccatgt gccgtgattt ttccttcgtc caaagccgtt aactgatgca atgtttcggt
     8101 tccactggct actgcagctt taggtggctg acttaaccaa gtaagttgcg aaatgtcctt
     8161 tacgccccag tttgccatat ccaaaactaa gggagctaaa tcggcatcga gaatttcggg
     8221 aatgcgatgt tctgccattc gtccctgatc agccatactc cacatgcgat aacacacgcc
     8281 agcactcaaa cgtccagcgc gtccagcacg ttgatcggcg gcatctttag agattttcac
     8341 tgtttgcaat ttcgacaatc ccgaagacgg atcaaaacgt gaaacacgtc cgaaacctgt
     8401 atctaccact atgcgaattc cttcaatcgt aaggctcgtt tctgcaatag aagtagctaa
     8461 aacaaccttc cttctcccct gcttgttcgg cataattgca aggaattgtt cgtgcggagg
     8521 taattgtccg tatagtggat gaatagctac gcctggcatc gatttactta gcaattccgc
     8581 acagctccga atctcgcctt ctccaggaag aaagaccaat aaatctcctt cgttttcctg
     8641 actggctttg agcaccacac gggcagttag ttcgactagc atcatctcat cttgtgtgcc
     8701 ggtgtagcga atttcaacgg gatattgtcg ccctttactt tcaatgacgg gcgcttgcag
     8761 caatccactt aagacgggca aattgagcgt tgccgacatg ataagaatgc gcaaatcggg
     8821 acgtaaaact agttgtgctt cacggcataa agccaaggcc acatcggcat gaatgctacg
     8881 ttcgtgaaac tcatcaaaaa tgactaatcc aacgccttcg agcgcattat catcgtgcaa
     8941 catgcgggta aggattcctt ctgtaagcac ttctagttgc gtgtttgctg acacacggtt
     9001 ttcgaaacgc actcgataac caatcgtttt tccaacttct tcacccatca aatcggccat
     9061 acgttgggca atagttttag cagccaaacg gcgaggctcc aacatcaaga ttttttgtcc
     9121 gtttagccat actgcatcga gcaaggtgag cggcaagagc gtacttttcc ctgcacctgg
     9181 aggagctgta acaattagcg ttgtgcgttc ttgtaactgc tgttgaactt cgtgaatgat
     9241 atcggctacg ggcaatccag tagcaatggg atctattggc atgttgaaat tgggaggcga
     9301 aattagtcat tgaatagcct ttccacacga ataaacagca ataaaaaaag ggcatctgaa
     9361 aacaaatgcc ctttgattga tagagtgaat tgtttttaat tcagcataag cttcgtgtga
     9421 tgagcaccat ccaccgaaac gatataaagt cctttagccc aatcgctaac tggaatttgt
     9481 agtgaattaa cagcatttga attcatgcgg aggattgatt ttccagacaa atcggtaata
     9541 gaaatgaaat gattttcggg gctaagtttg agctgtacta aatcatttgc tggattggga
     9601 aatacttcaa atgttgatgt tgattgtttt gatgggatag ccgtaattac aggactttgt
     9661 cgcatccgtt ctgcatttct acaatcacca aaattgcgca ttaaatcgaa atacattcca
     9721 tccgtaaaac tgaaattagg attggaaaca ataactcctg tatgaatatc tacgccaatt
     9781 aattgatcac cattactgaa ataatacgtg tcggtagaag ggtcaattgt tgccccagca
     9841 ttgagcgaat aaccgccacc taataatgaa gcatttgaaa taaccgaaac gtttccagta
     9901 gcaatatcaa tcttaccaag gcgaacagtt gcagaatcaa aatattgact ttcaaatccg
     9961 cttatagaat cgatttcaat ggtgtagtag tttctcctaa caagtccgta aagagatgta
    10021 tcggagcaac tgtaagtgaa attatcgaaa ataattccat cagtattttg gataggtgtg
    10081 ttgctgtata cttctccgga gtataggtca agtccgagta tgttggaacc tattgagaaa
    10141 taaaacacca tttcataagg attaatagca cttccagcta atgcgaaacc ttgagcaaca
    10201 gaagtaggtg aaatttgagt tataacacct gttgttgtat tgattttggc gagatatact
    10261 tcaccagtgg atgttgcagc gttgtagcga cgcgctaagc catacaaagt tgaatcggaa
    10321 ttattgaaac ggaaattatc gaaatagctt tctgcgattg gattcgaaat ggtaacccga
    10381 ttgaggactt ctccagttgt aagagaaatg acaagcattt gagaagcacc catgaaaata
    10441 taagtactgt cgtatggatt caatgccgca ccggttagat tcaccgcttc attcatttgc
    10501 tcctcgccta aactggtcac taaaccagta gcatgatcaa ttttccccaa acggatgcta
    10561 gcagaatcta attgctggta gttaaaatta gtatcaattg gatcaatcac ataactatag
    10621 taattcttgc gtacgacacc gaaagtgctc ccatttattt gagaataagc cacagagcaa
    10681 agagctacaa aggagcataa taaatagatt ttcttcatgc aaatagggtt aattttctca
    10741 taaacacgtg tgtgaaattt ttgtttaaaa taatcaataa ttctgtgctt caaattgttg
    10801 ttagcgaaat aatcgagtaa gtttctgact tagtttggat aatttggtta gagtcattat
    10861 ttacttttaa catcccaatt ctgtttgtct atgatgtttt atttccgatt tctaattgca
    10921 tttattttac tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn naattctgtt
    11041 tgtctatgat gttttatttc cgatttctaa ttgcatttat tttacttcac tcaacaatcc
    11101 aagcacaagg tcctgttata attggttatt ggcaaaattg gatcagttct gaagccccct
    11161 atattccact ggatcaaata gatggcagat atgatatcgt ttgtgtatcg tttgctgttc
    11221 ttacttcacc agcggatatg aatatgcaat ttgtccccga atctgttagt cagagtacat
    11281 ttattcagca aatgaatacc attcaagcac aaggaaaaaa agtcttgctc agcattggtg
    11341 gtgctaatac gagtatttct atgcccgacc aaacgagcca tgatgcgttt attaatagca
    11401 tgaattcatt attagatacc tatccatttg atggcatcga catcgatatt gaacatggaa
    11461 atagcatact cgcaagtggc attattagca atcctacctc agtcgattgt gtgtatttaa
    11521 ttgatgccat cactcaaata aaagctcact atgctgcgac acattcaaat actatgatgc
    11581 taacatttgc tccagaaact gcttacgtac aaggtggtat gtcggcttat ggtggaattt
    11641 ggggtggata tttacctatt cttgatgccc tccgagatga cataaattat attcatgttc
    11701 aattgtataa ttcggggacg gtttatggaa ttgatggaga aatatatgca caaggaactg
    11761 ccgattttat tgtcgctatg acagaagcca tgattcaagg attttctact tcaggtggag
    11821 aatttgcagg atttccagag gagaaaatag tagttggatt accggcatgt acagctgcag
    11881 ctggcggtgg attttgtagt ccagtcgttg tagcatcagc cattgactat ctccacggaa
    11941 acggaaatgc gcctggaaat tacaccttag ttcattcgca aggatatcct caattaggtg
    12001 gaatgatgac ttggtcgatt aattgggatg cggtttctac ttgtaatgca agtccttacg
    12061 aatacgtgag taattacgag caattatttg agacgatatt atcaaccgaa gaaaatggaa
    12121 aagaaataaa accggtactt tttccaaatc cagcaaacac tttcatacat atttcgaatt
    12181 taactgatag cgattatcaa atttctgata tcactggaaa gcttataaaa gcaggaaaaa
    12241 ttgattcaaa ttcaactatt gaaattgatt cacttaatga cggtttttat tggatatacg
    12301 taaatcaatc ttggataaaa ttcgtgaagc agtcgtcaaa ttaaatatgg aataattaag
    12361 ctttaaactt tggaagaaaa tcgttttata gtttctaata attcgtcttt ccttctacgg
    12421 gcaactggaa gatgatttcc atcacgcatt acaacttctc cgccttctcc acgatgatat
    12481 tcctttaaat gttccaagtg aataatgtag gaatgatgta ttcggaaaaa taattggcta
    12541 ggtaaaatat cttcaaattc acgtaatgtt ttagaagaca atattttttg atttaaggtg
    12601 tgaatataag tatagtttcc atcagccgct aaataagtaa tatcagagaa attcagaaac
    12661 aataatcctt gtccggtagt aatagcgagt gttttaggtg tttgtccttg ttgtagattc
    12721 ttgagtaaaa actctaaacg tttatcctga gattgatttg ctaaactaga ttttactcgt
    12781 tcaacagctg cttttaaatc atctacatca attggtttaa ctaaataatc aatcgcactg
    12841 aaacgaatgg cttgaatagc ataatgatcg taggcggtgg taaatatcac ttgaaaggaa
    12901 cgatctatta cttgttctag aaaggagaat ccattcatca aaggcatttc aatatccaga
    12961 aaaactaaat ctggattata ttttataata gcatctaaac cttcttgagc agagttgcac
    13021 atgcaaacta aatccacatc ctgacaatgc attacgattt tattctgtaa tgcttcacga
    13081 ctgggacgtt cgtcatcgat aagtatagct ttaatcatga atttctatgt ttgtagacga
    13141 acaattacac gcgtgccggc agcacttcca tcaggttgat aaagatcgat tgtttcaata
    13201 gaactacttc ctaatcggga agtataaaag gcaattcgct ccgaactaat ttgaacacca
    13261 agagaaggtc gatctttcga atgagctgat tttattttca tagcagcatc acgaccaatt
    13321 ccattatctt caatcgtgta aatcagctcc tctcctttta cttgagctat gatttttaaa
    13381 tacttctcgc ccgacatttt atgaagcaaa ccatgatgca atgcattctc aataaaaggc
    13441 tgaattaaca atggaggtac tcgataatca ccttgtaaca attcagcgtc tgcttcaaat
    13501 gtcaaattaa tagaaccatc ctgtcgaagt tgttctaatt ccagatatat acgaatagtt
    13561 tcccaatctt ttcgaaatgg aatttcagtt gaacgcgagt gttcgagcac atctcgaatt
    13621 aatcgagcaa atcgatgtaa atatttggct gcatttaaag aatcattttg ttgaattaga
    13681 ttatcaatag aattcagaca attaaatata aaatgcggat tcatttgagc gcgaagtgct
    13741 gccatttcgg tttcagcaat acgctgtttt aattgagctt ttgaacgaat tgcaattagc
    13801 cttctacgaa taattaaata agcaataact gcaattaaac taatacataa aatcaggaac
    13861 cagccttttt tccaaaacgg agtttgaatt agaaatttcc acgtcactgg tttgctccat
    13921 gtatggtgaa tgttggcagc acgtatttga atggtgtagt cacccggcgg tagattaatc
    13981 aaattcaact cccgttcatt ggtatttttc caagctgtat ccgaattaat taaatattga
    14041 aattgtagtt tatctggcaa tgtataatcg attgctccaa attgaatggt gatagaattc
    14101 tggtctgaat tcaacattgc tttttgagtg atagacattg aactatcacc caataataca
    14161 cctcgaatat atacttcagg atatttaatt cccgttgtaa aatgcttttc acgaatctga
    14221 aaataaccat tcatggttcc aaaaagtagt tctccatttc gcattcgaga aggcttaaac
    14281 acttcttgat cagtccatcc cgactccatt ccaaatgatg aaaatgattg tgtttgggga
    14341 tcaaataaat taatcgagct tggacaagaa acccacattc ttcctttctt atcaaatgaa
    14401 caccaatagg taaaattgga tgaaatccca ttttgctgat tgaaatgtgt cattgtattt
    14461 tgctcaactg aaaaatgagc caagccattg ctagcagtac aaatccatat tgaaccatct
    14521 gttgctaatt ccaaatctat aatcggatat ccgggtaatg aattaggctt attctgctgg
    14581 tattttaacc aaaaagagga attgctactg agatttaaac aatatacacc ttgattttcg
    14641 gttgctaccc agagtcgatt gaaatttgta tcaaatttta ttgcttgata cctacaatct
    14701 gtattagtgc ttggaatagg aatgattttc ttaaacttta aatcgggtcc aatttgaaaa
    14761 accccatctg tccaactaca ggtataggca aacccattgt catcaaaaac aacatcccag
    14821 aaaactcgat ttttactatt aacgggcgat tctggcagac taattcgaat ccattgagcg
    14881 ctaattgcat cccgaacgat tacttcattc ctcatacaaa cccataaatt ccctttggga
    14941 tcttttcgaa tggctgaaat aaaattgata ggaaactgag caatttcttg ggttgaatgc
    15001 gttttcagca attgcttatt taaagaaatt agttcatttg tatatatgga taaagcttga
    15061 aattcatctg taatttcatt gtcgataaac tgcgtataaa atcgatttac accgggaatt
    15121 tggataggat aaaaatcaaa aacgggttca tctaaagaac gtaaaacacc atgttgacag
    15181 gcaatccata agcgatttgc tttcttatcg ataaaaagag cttgaggaga aattggcaat
    15241 ggttcacctt ctaaaccatt gatggataaa ctagtaaact gttcatttat tggattaaaa
    15301 actataacat gatttcggct aattcctatg attttatcgg gtgaaattac ttgaagtgaa
    15361 tataccgatt gcaggtgatg agaatcgagt aatgaatcgg gtaataacca agatttccaa
    15421 gttttacttt ctatatcaaa ggctacaatt ccattattat aggagcccat ccaaagttta
    15481 ttaaatgcac ttatgtatag acttaaacct tggaactctc gaattaaatc gcttggataa
    15541 taattaaata aatgggtttt ggttgaataa ctaaataatc cattggttgc gagtagccaa
    15601 tattcatcac ccgatttagg aataattgaa tttccttgta acaaaggttt aaaaagtgga
    15661 atttgatttc taacatactg acgccaacca gttgaacgat atttccccgt ttttaaatct
    15721 aacaatagaa aatcacccat tgctccaatg atggcttgcg attttcgcga atctacataa
    15781 caggaaataa tctgattaga tatgagtgaa tcgggcaaat gataggattc aaaatcaaaa
    15841 ccttgattaa ggtgtttgga taattgattg ccttgtacaa cccataaaat accagcttca
    15901 tctaaaataa tatccttaat ggcaccagat tgtatggata gctttttgtt attattttga
    15961 agtatagaat taaatcgatt gccatcaaaa cgtgctagtc cgtttgtagt taaaacccaa
    16021 gtaaatccat ctttatcggt caatacttta ctgaccacat tcgaaggtaa accatcgcgt
    16081 gttgaatagg catacatccg gttgctaaat ccttgagcgc gtgcatgctg acaagtggat
    16141 aaataaatta ttatccccga gattaagcat atcaactgtc taatggactg cattggctaa
    16201 atataccatt tcttcatgcc aaaattcaca tgtacaaatt cacttataat gaagaaagta
    16261 attggtgtta ttgcttgata aacttgaatg ttttactaga cttacttgtt tgaacattaa
    16321 cgatataaat tccagtattc aaagtcgaaa catctaagtg cacaaattga gcttgggatt
    16381 tgtattcgtt aagcaattga ccagtaattg agtaaagctg aattaattca aaatcttcat
    16441 cggatgtatc atttcgaatg gtaagtttat caactactgg atttgggtat aattgaaaat
    16501 ttgaataatc attagttgga atgtttgtaa tggctgatac atctaattgt aaaaaaccaa
    16561 attccgcaaa tccaccttgc gtttcaaaag attgtccgcc aaatagtaat ttaccatcat
    16621 cttgtatcag tatatcggca attccactgg aataggttcc cattttcatt actaatttac
    16681 catccgagct aaaggtatta tcaaggcttc catttgcagt atatctagca atagcgaaat
    16741 ctaaaaacgt attaacagaa ccatcgtaac catgtccagc aagaataatt tttccttgtg
    16801 catcaatttt caaggcataa gccttatctt gataatcatc aaaatcggtg gttatatgcc
    16861 cattggagcc aaaagaagca tctgaaacac catcgatatt catacataat actgcaaaat
    16921 caccacctgt gtaacctgcc gaaatgattt taccatttgg atgtaattcc atgacttcaa
    16981 ccacttcgtt gtaatttgaa ttaatattta aaatgagctt tccattgcta gcaaaaccgg
    17041 tatctattgt tccatcttca agtaatcgaa tccaagcaaa tgcgctattt ccatcactat
    17101 tataagcagt tccaccaatg agtattttac catcctccaa caaaagtgca tcgtatccaa
    17161 aatcgttaaa tccatttaca tcgtgggtga ttaatccatt tacaccaaat gtactatcta
    17221 caacaccgtt agcatggaat cgagctgcag caaaattgaa atttgatcct ccatatgctt
    17281 caccaatggc tacaatttta ccgtcatttt gaatgagtat tttgtgtgct tttgaaccat
    17341 taccaagccc aaacgagagt tttacttttc catctgtact aaacgtcgta tcgaaatctc
    17401 catcgggtaa aagtcgagct agaaccaaat ctccattgga agatccacca agtaaaattc
    17461 gatcttgact atcgattaca agtgcatcac aaaactcata ggtgaatccg aatccaaatg
    17521 tcaaaatacc tgttccttgg aatgtagaat caagcgaacc atcgtttttt aatcgacaaa
    17581 caacaattgg atttaatata ttctcattgt cacctactgc aataatttta ttcgaagatt
    17641 ggaattccat ttcttcaaaa taataagaag taaaaccaaa tgaagcaaag gctttaccgc
    17701 tatttccaaa tgttggatct aattcttgag cagacagttg aaatgaaaaa aacagggaaa
    17761 aaataattcc ggtgaaaaag attttattca taaaaatgct tgatttaata attagtgtaa
    17821 cacttgaacg ctacgtgaat actggtctgt attggtattt acttgaagaa aataaatacc
    17881 tgaaggcaaa gaattaacgg atattgaaaa cgtagaaatt aaattcacga ttcctgtttg
    17941 aactttattt ccaagtacat caaatagcgt ataatgagca tcggagagct cagtatccat
    18001 ttcgattgtg atctgttcag ttgcgggttg agggtatatt ttcatgtgag ttgaagaact
    18061 attttttatt cctaaaggca aactctctat tccgaattta actacccaaa aatcatcatc
    18121 accatgattt ccagtaacat ccccatcaat tgaattggtt tctcctacca tcacccaaga
    18181 cgaatcattc atccttctca ggcaatacgg acgttctaat ccggagccac cataccagct
    18241 ttcccatgta ggagttccat ctgcagaaaa tgctttcaaa accatatccg ttccaccatg
    18301 gggcgtcaat tctcccggcg attcaggtgc ataaatttca ttggctgtca cataggaacc
    18361 atcgctcaac tgaattccag aaaaactacc atcgaaacta attccaccaa aacattgctg
    18421 ccattcaaca attcctaact gacttaattt cgccacccag caatcgtaaa acccgctatt
    18481 actagtcaaa tcgccatcag ccgaagagct ttctgctaat aataaatatc ctccatcttg
    18541 agtaggaata attcctcggg gacgatccat ggctgatccg ccaaaaactt tagaccattc
    18601 cattgcacct gtataatcgg ttttaataac ccagatatct tgttgtccaa gatttgttgg
    18661 aacatctcca tcatccgaat tcgaaatacc tgcaataata aaaccttgat cgttcgaatt
    18721 tgcaacagca taggcagttt cagcacttgt tcctcctaat aaaacaatat tctcaactgc
    18781 accaaaaaaa cttattcggg caataaaaac atcactcaat ccatgatttc cacttatatc
    18841 gccatcggtt gaataagcat ttccaactac aataatattc tgccccgaaa ttgtcatccc
    18901 aaatagttct tcgttatccg atcctccaat ggaaagatcc caaactaaat caccatcaac
    18961 cgataatttc acaatccatg catcgtaagt aagtccgtga ttcatactca catccccatc
    19021 ttgcgaaatg gtatgtccgg ctaaaacgta cgattcgtcg ggcaattgaa caatatcaaa
    19081 gccttcatct tgataggtac cgccaagtgt tttagtccat tctggctcgc ccgattcatc
    19141 taatttcact acccaaatat cactgctgcc atgatttcct tccacttctc catcgtaaga
    19201 agtagtggat ccaattacaa ttaaaccacc atcagcagtt ggtcttaccg atttagcatt
    19261 ttcataggaa gctccaccat agcaacgtgt ccagagggta tcaggtgctt gagaaaatgc
    19321 ttgaaaaaag aaaccgaaaa ataaaatcga cactgaataa cgcattcgaa aaatgaattt
    19381 agatgcgcta aattgaatcg accttgttta attaaaatgt cagaatgagc ctgtggttaa
    19441 attgaatgat taagtggtag aattagctca atacttcgaa tccacaacta atcgaatttc
    19501 cttgatcatc cactaaagtg agtttgtgtt taccaggcat gggttgcaat tgaagctgat
    19561 ggatatcgcg tgttgaacca ataaattcgt catctaaatg ccaataaatt acagcatctg
    19621 aacgacgatg attagcctcg aaaatggtag ctgtcggaat accttctaat ccaattggaa
    19681 ccgaaatccg gctatttttc cttggataaa ctaatgcgat attttgttct ggttcaggct
    19741 cgcaacccgg tttccaagca ggtagtggag catacgtagg tgtatgttga gcgtaataat
    19801 tttccatcac cggaggcaat acaaaccaat tcacatgtcg catcgaacca acatcagcac
    19861 aggaactatt tacgcgcaat ggctcacgag catctaaatg tatgcgctga tgatatggac
    19921 aagctttgct atacatggcc gattcaggta atttaagaaa tacagaatcg gagcaatatt
    19981 ctgtcaagcg gaaaccactt tgagcacaaa taggaagtgt tttcatttta tcgacaggtt
    20041 tggtaaacca tccatcgtat ggaagtttgt cgaaaatatc aaacaataca ggtgcggcac
    20101 aattaattcc cgtaagtccg ggacgccctt caccacttgc atttccaacc caaacaccaa
    20161 ctacatatcg tggggtaaca ccaatgctcc aagcatcccg aaagccatag cttgttccgg
    20221 ttttccaggc aattttcctt cctcttccta agtctttcca ttgagcttct tcacccggac
    20281 gatttacttc gaccatcgca tccaacatca accaagcaga tgcgggagaa aagccagatg
    20341 taagtgtgtt tttagcatta gatttccctt gaatataaga agctacatgt ggatcgttgg
    20401 actgtttacg ctcatcggca cggagttcac gagcaattcg agcataggca ttacacaaat
    20461 cccataaact cacttctgca ccaccgagaa ttaaagctaa accataatgc gaagcaggac
    20521 gattgattgt actaagtcct aatcgacgta atttctgatg aaatttttcc acgccaaaat
    20581 cacccagtaa acgaaccatc ggaatgttta aagatcgcgc caatgcgtct tttgcaggta
    20641 cagcaccatc ataggtatga ttaaaattct taggcgaaaa acccgaaaga tgggttggaa
    20701 tatcgggcaa taaactgatt ggagtaatga gtccttcatc caaagccgat gcgtataaaa
    20761 gtggcttcaa aatactaccc gaacttcttg gagctcttat cacatctacc acatcgccca
    20821 ttccgtgtcc atccgaataa tcactaatat tccccacata cgatatgact tcattggttt
    20881 tcaaatccag aaccagcgta gccatgtgat gcacatcatt tgacttttgt cgcatggtgt
    20941 gcatcatcat aacctgctga atctgctgct gtaattcacc attcacggtc gaatagatca
    21001 cagtagcacc atgaccttcg gcaattgctc tatccaaaag ttgggtagct aattgtggaa
    21061 ttggaaaggg ttgttgaggc aatggttctt cgagtgctaa tgctaaatct gtatcatcca
    21121 agaaattcgc ggtatgaagt cgatttaata atcggttccg cttagccaat aatcgatgtt
    21181 gatttttacc aggaaatatc aaactgggag cattgggcaa tactgctaaa gtcgccgatt
    21241 ctgcccaact taacttatct gccggtcgac caaaatatcg ccacgatgcg gcatccaatc
    21301 ctactacatt ccccccaaaa ggtgcattag atgcgtaaag cgatagaatt tcttccttcg
    21361 tatagctcag ttcaattcgg gttgcccaaa tactttcaat cagtttatcc catatgccgc
    21421 gatccgaatt tttccgcgac atacgagcta attgcatcgt caacgtactt ccaccgcttc
    21481 taactttcat gctctgagaa ttctgccaaa cagctctccc aactgctttt ggactaactc
    21541 ctaaatgatc ataaaaatcg cggtcctcaa actgtattaa tgcctgttta aatcgatagg
    21601 gcacttgcga attataggga aaacgccatt gtccatcggc tgcaatcctt gccgatagca
    21661 gatttccatt tctatcctcc aaaaccgtac aagctggatc ggcaaataat tgattaggca
    21721 aacaaaatgt gtacatccca agcaggacaa agaaaagctc gggcaaaatg aatttcaaat
    21781 agcgttttac tcgtttcatc ggacaaaatc tgaaacgaaa atgcttcatc caattttaca
    21841 tcaatgtatg tttcgagaat ctgcaactat ccaattagaa ttcgcttgta tatgactgcg
    21901 tatgccatag cctcggcgaa ggcataaggc ataaggcata aggcatagtt ccggctcaac
    21961 cgctcatgca aatgccgtta taattttcta aatcagtgca tgatgtattt agcattaatc
    22021 tatcaacagc tatgttaata tttcgaaaaa ttctgattta acagtacttt cagcaagatt
    22081 cttagagtga atttttatga acaggcttgt caaaaactaa ccgatcttaa atgtttaaca
    22141 gacgaatcat gtgctgcgga ttttgtttct ttcagccggt gggtggttag tggtcggccc
    22201 ccgcgtgcgc gagaagtatt tcttgattaa caaggtttat agtaaattct cgttttacaa
    22261 attccaaacg ttttccctaa aaaagacttt tcccccatca cttccctcct gctatgttcc
    22321 tacatttgtc ggaatacatg ctgcgccttt tcaaaattct acttcttctt ctgctctttc
    22381 ccttaggaat tagagctcag cagtttcatt ttcgtaatta ctcgcttagt gaaggattag
    22441 cccaaagtca ggtgtatgct atctttcagg ctaaaaatgg aaccatttgg atgggcaccc
    22501 gtggtggtgg attgagtgct ttcgatggta tacgatttca aaattataca actcgtgatg
    22561 gcttgtcaga caattacatt cttagcctag cagaagattc tcttggaaag ctctggattg
    22621 gtactaacaa tgggatttgt acattcaacg gtagaatctt tcaaaaagaa actctacccg
    22681 atagcggaac tatcgtagta gaaagtattt tattgaatcg cccaaatgag attttaatcg
    22741 gaactcagaa aggaatttat tcatacaaat caaataaatg gacaaagttt agagctgaca
    22801 ttcctagtat ccaagaaaac attagttgcc tatacaacga tcattcaaac cgattgtgga
    22861 taggtaacaa tagcggaatt acatgcatcg ataaaaaaac ggtctttcat ctgggattga
    22921 aagaaggctt gccaagtgaa gcaatacgtt gcataaccga agatgccggt gggacaattt
    22981 gggtaggtac gtatggaagc ggaattgctt ggattactaa aaatggtatt cggataataa
    23041 attctactaa tgtcctacct gatccaatag ttcaaagtct ttttcgtgat gtacaaaata
    23101 gaatctggat tggtactcaa gaagcaggag ccggattttg gaatccttcc gatagtttat
    23161 ttcgatttat taatgaacga gatggattaa gtaataatca tattcgctgt atcatagatg
    23221 attactgggg aaatatctgg ctcggaacat ctggaggtgg tgtaagtaaa tatttcggtc
    23281 agcaattcac tcattttgat caacgcagcg gactagctgg aagaaatgtt tatgcaatcg
    23341 cagaagatac ttcaggtgta atctggttta gcacttctgc tggtggcttg gggcgtgtag
    23401 aaggaaatag tatcaaagat ctaagtcgtg ccgatggatt tccaacagta aaaatcaaaa
    23461 agttaatgac cgatttacaa aaccgtttgt ggattggtac cgagggcgaa ggtttgtaca
    23521 tgtattcagg ccaagacttt aaacattttg gaaatgaaaa tggattaaaa ggaaattgga
    23581 tacgtgatat tattcaagat ttagctggga atatttggat tgcaaaagcc ggtggcggaa
    23641 tagcgaaact aacagaagaa aatacccagt ttaaattcaa acaatttagt ctagaagaag
    23701 gcttaattga agatcgcatt aatgcattat tacaagataa atctggaaga atctggtttg
    23761 ctactacttc taccggatgt ggatttattc aaaatgattc tattattaat ttcagcacta
    23821 aagatggatt aagtagtcaa aaattacgct ctttagcttt agctcctaat ggtacaattt
    23881 ggataggaac tgctgatcgc ggcttgtgtg cgattagtat gaatggcaat aaacctgtct
    23941 gtcaagttat ttcatccaaa caaggactta catccgataa tatttattta ctcaatttcg
    24001 atagtgatgg aaagctttgg gctggttcag aaaaaggact tgatcgttta acatttagca
    24061 aggaaggcaa gctcgaagaa atcaagcatt ttggttattc agagggtttt attggcattg
    24121 aaacatctca aaacgctacg ttacgcgata aacaaggtaa gctctggttt ggtacaatca
    24181 acggattaac acgctttaat ccagcactta ccgacattaa caaaatagca cccattcttc
    24241 aattacgaga tattcggtta ttcttcaaac cactaactga aacacctttt gcttcatttt
    24301 gggagacttt aagtcaagat acttctcaaa ttctgaaact tccatttaat cagaatcatt
    24361 taagttttga ttttttcggg gtaaatcatc gaaatccaga acaagtgaaa taccaatgga
    24421 aactggttgg gttagatgaa agttggacac ctcctggatt tcaacataat attacctatt
    24481 ctaatcttcc ccctggagac tatacatttc tataccgagc agcaaatgaa gatggaatct
    24541 ggagtgatgc aaaagcaatt cattttcaaa ttgctatacc catctggaaa cgctggtggt
    24601 ttattggagt ttgtgcaggt ttagtagtaa tccttgtctg gttatatttc cgttggagat
    24661 taagaagaat aaagcgtaaa gcgaaggaag cacaagaaaa actgaagctc gaaaccgatg
    24721 ttctcaaact agaacaaaag gctttgcagc tgcaaatgaa tccgcatttt attttcaatg
    24781 cgctcaattc cattcaagct ttaattggca cacaagacga aaaaacagct cgttattatt
    24841 tatccaagtt ctcccgcttg atgcgtcaaa tactagaaag tagcagagaa caaatcattc
    24901 cgcttgaatt agaaattagc attttagaaa attatttgag tatcgagcaa ttcagtcacg
    24961 gtgttaaatt cacgtgggaa ataacgtatg atgaatcact ctccccatca aacacaggta
    25021 ttttacctat gctcatccat ccatttgtag aaaatgcggt gatacatggt cttgtgccca
    25081 aaggagccgc aggacatatt cacattcaat ttagcaaagt tgatgagcaa ctaattgtta
    25141 gagttacaga caatggtatt ggtcgcgaaa aagcccgaga acgtcgctcc caacaagacc
    25201 aacatcataa atcaactgca ttattagtgg ttcaagaacg cctcgaatta ttagaaggaa
    25261 aaacgcatgt tcaaatcatc gatttaacaa acagtgacgg aacttcatca ggaacagaaa
    25321 tcaaagtgat cctacctttt acatctttag attaaagaat tatttgcagc tatttttaac
    25381 tattttcgct caaatgtttc taaataaagt tgtaattcgt aattcgtaat tcgtaattcg
    25441 taattcgtaa ttcgtaaatc gtatatcgca aatcgtaaaa aatgaaactc cgcgctctcc
    25501 taatcgacga tataactgaa gctcgcaaca atctgcgccg agatattgct gattggtgcc
    25561 cagatattga aataattggt gaagctgatg gagtaatttc tggtgctaaa gaaatccgta
    25621 aacaaaaacc tgatttagta tttctagata ttcaaatgca ggacggtagc ggatttgatt
    25681 tactggacat tttaggagaa atgaaattgc atgtcatttt tactaccgcc tcggatgcat
    25741 ttgctgtcaa agcttttcgt tatgctgctg tagattattt actaaaacca atagatcctg
    25801 acgaactgaa aaatgctgtt gccaaaataa ataatcaatc aaattctaac gaaaacctaa
    25861 gcttattaaa gcagcaatta tcatctaaaa aacaaaccga tcgtattgcc ctacatacac
    25921 ttgagaaaat tcatgtggtg acagtcgatg aaataattca ctgtgaatcg gatgataatt
    25981 acacgcgttt ttattttcaa gataaatcca aattattggt aacaaaaaca ttaaaagaat
    26041 ttgatgaact tcttagtccg cttggatttt tgcgagtcca tcaaagtcat ttgattaacc
    26101 tgaaacaagt aaaagaattc atcaaaggcg aaggaggtta tttaattatg aaagatggta
    26161 gtaacattcc ggtttcaacc cgtaaaaaac aagaagtaat tgaaaaatta attgattaaa
    26221 cttcattttt aataacacct tttgtgatta atctacaacc tacacttgaa aatgaattac
    26281 ttcgccttcg tcccttaatg gagagcgatt atgaagaatt atatcaatgt gcttccgacc
    26341 cattaatctg ggaacttcat cccaataaaa atcgatacaa aagagaagtg ttcgacgtgt
    26401 atttccaaga agcgattgaa ggtaaaatgg catttgcagt tatcgaccta aaaacaaata
    26461 aaataattgg tagcacacgt ttctacgatt ataaaccaga acaaaaatca atagctattg
    26521 gctatacttt cttaatccgc aattattggg gaggattgta taataaatcc atgaaaggtt
    26581 tattgattga ttatgccttt cagttttcgg atgaaataat attgcacatt ggagcgaata
    26641 attttcgctc acaaaaagcc acagaaaaat tgggagcagt taagattgca gaagaattaa
    26701 tccctttccc tggtgaagct aatgcgctca attttgttta ccaattaaaa aaatgatata
    26761 ctcgtttttc accttttgat gtcaactttt cacactatca aattatgaaa caaccacttc
    26821 cttccgaata caaaccctat ttccatcggt atattagttt agttgaaggt gaaaacttct
    26881 tcgaacaata cgataaaata aatcaagatt tcatccagtt tttgaattct atcccagcag
    26941 ataaacacgc ttatcgttat gctcctggta aatggactat caaacaaatc ctacgtcatt
    27001 taattgattg tgatcgtgtg tttgcttatc gagctctagt agctgcccga ggtgataatt
    27061 ccccacttca ttcgtttgac gaagattcat atgctttagc tgcatcagag gaaacctgta
    27121 gtttgaatta cctgattgaa gaatttaaag cagttcgttt tgctaaccgt tgcatgtttg
    27181 aacaactaag cgaaaaacaa tcgcaagctc ttgcaaatac agaagaacat ccaatgtcgg
    27241 taagagcaat tgcttatata ttaatcggac acgtaattca tcacactagt gttattaaag
    27301 aacgttattt gaacgaataa attccaaatt atcttaaata cccagttcag gaaaatggat
    27361 gagaaagcgg gatttgattt ggttttcagc tggttggtgt tctcaaaaat aaatctagaa
    27421 atattgatat gaactttaga aatgcgatct attcaacatt ctttttatta ttcatagcta
    27481 ttacttcgaa tgcccaagaa agtataaagg aatatatccc attagataaa tttctttatc
    27541 accaaattga ttcaatggat aaagtgtttt ttgatgctta caattcgtgt aacatagaaa
    27601 aacaggaatc tatttattcg gatagtattg agttttttca cgataaagtt ggattgataa
    27661 attctaagtc cgaaattatt caaggaacta gaaataatat atgtggtaaa gtaactagat
    27721 acattattcc gggcagtatt gaagtatatc caattaataa ttatggtgct gttgaaattg
    27781 gatttcatag attttataat aatcaagaac cagatgcgga gtcaaaacca agtaagttta
    27841 tcattatctg gaaaaaagaa gatgtaaatt ggagaattac taaagttgta agtctacatt
    27901 gaagagtatg ttaaaaatat gaatagcaaa gtcgggtatt tcaggatttt aaggtattcg
    27961 ttcattgaat aaactctcca atcctgatgc tttgagttta gctaacttgg atctgcacct
    28021 atgttcgaat agtaaactat ctgaaatgga ttgattttat ctattaacga ataataatca
    28081 agttgttatc tgatcaatat taactatgat ttctatcagg taaactaata atttgacagg
    28141 taattttgct tcaaatttca agcaatgaaa acggttaaat tcataagtgc tgatataaat
    28201 cagaaacaat ttgcagcgga agtccgaaaa aatgttttag cctatttcaa ggataataaa
    28261 atctccacca aaggtgatgc aggtctttat ttcaggacat tcctgatgat tgcttgttat
    28321 gttgttccgt tcattttgat tttctcttta acaattccta attggcttgc attcgtgttt
    28381 tgtatactca tgggtgtagg tattgcaggt actggaatgt gtacaatgca tgatgccgtt
    28441 catggtgcat attcgaataa aaaatgggta aataatttaa tgggtggaac tttatattta
    28501 cttggcagca atgtgtttaa ttggaaaatc caacataatg tattacacca tacttataca
    28561 aatatcaatg gcttagatca agatatagaa tcaaaaggac caattcgttt atcagataat
    28621 gcaccattga aaaagtttca taaatatcag ttcattcatg cattcctttt ctatggattg
    28681 atgacattag ctaagttaac taaagacttt actcagcttg caacatacaa taaagctgga
    28741 atcaccaaga attacaaatt caacccaact ttagaataca tcaaaatggt tgtattcaaa
    28801 ttagcctata tttcagttat tattggtttg cccattcttt atacccaatt tacttggtgg
    28861 caagttattc taggattttt cattatgcat tggactgcag gagctatttt aagcacagtc
    28921 ttccaaatgg ctcacgtagt agaaggtgcc gaacagcctt tacctgataa aaatggtgtt
    28981 gttaattgcg actggaccgt tcatgaattg aataccacat ccgattttgc accaaaaaat
    29041 aaattactaa actggtatgt tggtggacta aatttccaaa ttgaacacca catattcccc
    29101 aatatttgcc acatccacta ccctaaaatt gccccaattg tagaagcaac cgctaagaaa
    29161 tatgggtatc aatataatct gaagccaagc ttgtgggatg catttaaatc gcacgttatc
    29221 agattaaaag aactaggtca accattgcca gctatcgcta actgattcat ttaaagagat
    29281 cagctagtaa tttctgaaac gttatttttg ttcttaag
//
LOCUS       DAOOQW010000008        28439 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_1929_length_28439_cov_6.737810, whole genome shotgun sequence.
ACCESSION   DAOOQW010000008 DAOOQW010000000
VERSION     DAOOQW010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 28439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28439
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1929_length_28439_cov_6.737810"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /locus_tag="PK281_01205"
     CDS             <1..559
                     /locus_tag="PK281_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOY47984.1"
                     /translation="LCIFDVMMPQMDGFTLAKEIRNHNKDIPIIFLTAKSMKEDTIEG
                     FKAGGDDYMTKPFSMEELLLRIKAILKRTERVVDDGPVQEFIIGKYSFNPTHQQLTLG
                     DHIQKLTSRESDLLKLLCMNLNDTLERNFALRAIWNDDNYFNARSMDVYIARLRKYLT
                     GDPALEIINVHGKGFKLVDGHTPSS"
     gene            complement(560..1837)
                     /locus_tag="PK281_01210"
     CDS             complement(560..1837)
                     /locus_tag="PK281_01210"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOY47985.1"
                     /translation="MKNARAYRLLFIANAVSGLSQGICMLAIPWYFSKILGKPELFGM
                     MYAATTFIMLFWGLYAGSLIDRFSRRHVFMAVNAFGLLLLGSVSAIGFYQGSVPAWGA
                     MLVFAGTILVYNIHYPALYAFGQEITEKQYYGRFTSWTEIIGQSTSILSGSAAALLLS
                     GVSEGEHHFAGLTINFPFEIKPWTLQEVFLTDAITYLIGFFLIALIRYEAIAIRHQEA
                     GSALDRIRTGMKFLKDNPLIFQFGNASYAIFVVLLVTFQQLLPQYIDQHLHSGAGTYA
                     FAEVMYSIGAMLAGVFMVWTFRKSGFILGITVMMGITVLIYALFASSSNLLLFSILCL
                     LVGITNAGTRVLRTTWLFQHVPNQSIGRVSSVFQTINILFRAGISSLFAIPFFNRNDQ
                     IVYAFAVCAVFVGFWMIPILRNYKKLANFKMSS"
     assembly_gap    2098..2197
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2198..3208
                     /locus_tag="PK281_01215"
     CDS             <2198..3208
                     /locus_tag="PK281_01215"
                     /inference="COORDINATES: protein motif:HMM:NF012544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HOY47986.1"
                     /translation="KNILLDLGFLTVSTQQLTAILVIVFLSLLNLRDVKNSAIIQNFF
                     TVAKIAALLGVVAVGIYFAGQGKGNWQNMEPVWPENMGLGTIGIFLAALTGSLFSADA
                     WNNITFTAGEIKNPGKNLPLSLAIGTGIVLLIYILVNVIYLYMMPLADIQHAPEDRVG
                     TALMNSMLGSNGQFVMAAIIMVSTFGCLNGLIFTAPRAYYAMAKDGLFLKRATQLNKH
                     GVPAFAILIQLVWSSALCLSGKYGDLLDYLMFVVMLFYILTVGAVFILRRKMPDAPRP
                     YKAIGYPIIPILYILLATCICGVMLCFKTTFALYGLAIVGVGIIVYQFSSKKEIEDKT
                     AQ"
     gene            3210..3677
                     /locus_tag="PK281_01220"
     CDS             3210..3677
                     /locus_tag="PK281_01220"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HOY47987.1"
                     /translation="MPVIFTVTEAVEKMKKFCAYQERSQQEVRQKLYQSSIKGEEAEF
                     VIGELIRENFLNEERFARAFARGKFRMKQWGKIRIRMELQHKGVSEPCIRKGLSEISD
                     RDYRKTAEVVLKKLSGGVLPDSKKEAWKLLSKMRSKGFEPELLQSIMNDFSEE"
     gene            complement(3681..5060)
                     /locus_tag="PK281_01225"
     CDS             complement(3681..5060)
                     /locus_tag="PK281_01225"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HOY47988.1"
                     /translation="MSTLVLLSLLGFLVLFLGAFHQKKLLLPVAILGLVAAFITNALD
                     WGTNHLWYNMMLYDNYAVSFTGLMIVTLILILLIASYHFRNEDSHVADIYALMIFVLV
                     GALVMTSYSSLVMLFVGIEILSIALYILAGSRRLNLASNEASLKYFLMGAFASGFLLF
                     GITLMYGVTGSFDLAAISDYANGTDNLPLIFNTGVVLILIGFAFKIGAVPFHFWTPDV
                     YQGAPTLITAFMATVVKTAGIAAFYRLFDTCFSGIDGVWYNILWVIAVATILLGNISA
                     VMQSEAKRMLAWSSVAHAGYLLLPVLALNELSSSSIFYYTAAYSVASVTAFGVLIAIS
                     QFNNEMPVDNYKGLAKNNPMMTLALVVSMFSLAGIPPLAGFFGKYFVFLTAMESNLNI
                     AVIIAIAGSLIGIYYYFRPVIFAFNNDGQPMERIQTSWTFNFVLALGVFLTLLLGIFP
                     DLLANLPGN"
     gene            complement(5062..6540)
                     /locus_tag="PK281_01230"
     CDS             complement(5062..6540)
                     /locus_tag="PK281_01230"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HOY47989.1"
                     /translation="MITAALIAFPFLAALMLFLLPANAAKKGALVATLIQAAFTAYVI
                     FLFDINEHRSLLSNEQFILSENWISTMGIRFFVGLDGLSMLMVILTNFLLPLIVLTTW
                     NREFRNPRLFYFLMLMMQSALVGVFVSLDAFLYYIFWELALIPIYFIVLIWGGENRVK
                     ITFKFFIYTLLGSLLMLAAIIWLYLQTTDRSFGIDAFYALRLDPVAQRWIFWAFFIAY
                     AIKIPLLPFHSWQPDTYTTAPNAGSMLLSGIMLKMGIYSIFRWILPITPMAVSSYTDV
                     VLIMCVVGIIYASWIAIGQKDLKRLFAWSSIAHVGLITAGIFTLSRTGFQGAMIQMLA
                     HGVNVVGLFFVAEIIQQRTGTNEIAKLGGIRQLAPVFASCFMVITFASVALPLTNGFI
                     GEFLLLSSLYDYSYVFAAFAGLAVIFGAVYMLRAYKTSMLGENPAITVFADLTISEKL
                     VLFPIVGLIVFFGLFPQFIFNLTDESVKYLLTEISNHMVPNL"
     gene            complement(6543..8465)
                     /gene="nuoL"
                     /locus_tag="PK281_01235"
     CDS             complement(6543..8465)
                     /gene="nuoL"
                     /locus_tag="PK281_01235"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014083212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HOY47990.1"
                     /translation="MKDLAWLIPALPLIGFLFSIFFGKRLPKNLVASVASVAVLVPFI
                     LTVSIFIPFVNTLDAEPIRILLGEWIQIGKLSVNYSFQLDQLSLMMMLVITGVGFLIH
                     VYSAGYMHDDPGFSRFFAYLNLFIFFMLILVMGSNYIMLFIGWEGVGLCSYLLIGFWY
                     KNPEYNKAANKAFIMNRIGDLGLLLGIFLLYLRFSTLEYNRISLEALDIMSGDPIIRN
                     ATLLLFVGAIGKSAQLPLFTWLPDAMAGPTPVSALIHAATMVTAGVYLIIRSNVLFTL
                     APFTQEIIMIVGLCTALFAASIGLKQNDIKKVLAYSTVSQLGYMFFALGLGAYSAAYF
                     HLFTHAFFKALLFLGSGSVIHAMGGEQDIRKMGGLKKALPITHFTFLIGTIAIAGIPP
                     LAGFFSKDQILASAFAHNPVLWILGVCGALMTAFYMFRLYYLTFFGSFRGTHEQEHHL
                     HESPKTMTIPLVILAICSCLAGFVNIPEVLGGTHWLDRFFEPILAGSSLILEDFGVEH
                     HLSHSLEIILMVISVSAVLGVIGYAWIRYKKNGHIPAAEGEHITAFEKLLVNKYYIDE
                     LYDSVVANPVVSASKGIYKFLEVNVIDRIVNGVGQLVVLGSRTARFLQTGNVGFYIFA
                     MVIGVLVILAANSFLN"
     gene            complement(8488..8817)
                     /gene="nuoK"
                     /locus_tag="PK281_01240"
     CDS             complement(8488..8817)
                     /gene="nuoK"
                     /locus_tag="PK281_01240"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004918357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HOY47991.1"
                     /translation="MEELQNSFREIGIHHYLFLSAILFSIGVMGVLLRRNFIIIFMSI
                     ELMLNAVNILLAAFSRFHGDQSGQVFVFFIMVVAAAEVAVGLGILVMIYRNIRSTDIN
                     ALRNLKW"
     gene            complement(8820..9317)
                     /locus_tag="PK281_01245"
     CDS             complement(8820..9317)
                     /locus_tag="PK281_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020213325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HOY47992.1"
                     /translation="MTPIAFLILAALTIGSALMVVFSKNPIYSVLWLVICFFSITGHY
                     ILLDAGFLGIVNLIVYAGAIMVLFLFVIMLLNLNKETEPHKPMLLKVAAVFSACLLMI
                     ILAASLREYDTRPATMITEEFGTAKGVGKVLFKDFLLPFEVASILFISAMVGAVILGK
                     KHVND"
     gene            complement(9372..9899)
                     /locus_tag="PK281_01250"
     CDS             complement(9372..9899)
                     /locus_tag="PK281_01250"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HOY47993.1"
                     /translation="MQLTNRSKVVSDKNMTLAEGSYLPAVLKGMAVTLKHFFKRPATI
                     KYPEQTREFSEVYRGKHVLKRDENGAERCTACGLCAVSCPAEAITMVADERKKGEEKL
                     YCEEKYAAIYEINMLRCIFCGLCEEACPKQAIFLTKEIVPSDYDRDDFIFGKDRLVEG
                     VDASKRIDITKRQDR"
     gene            complement(9932..10960)
                     /gene="nuoH"
                     /locus_tag="PK281_01255"
     CDS             complement(9932..10960)
                     /gene="nuoH"
                     /locus_tag="PK281_01255"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HOY47994.1"
                     /translation="MDMLFLLDKTVIAFIAFAITLLIATYSTYAERKIAAFMQDRVGP
                     DRAGPFGILQPLADAGKFFFKEEIIPNASDKFLFIAGPGIAMLTALMTSVVIPWGDTL
                     NIAGREISLQVSDLNIGVLYIFGVVSLGVYGIMIGGWASNNKFALLGAIRASSQMISY
                     ELSMGLALIALIMMTGTLSLKEIVDQQHGMNWNIFYQPFGFLLFVVCAFAECNRTPFD
                     LPECETELVGGYHTEYSSMKLGFFMFAEYINMFISSAFMASLYFGGYNFPFMDSLGLD
                     QNIITIIGVFVFFLKIFAFIFLFMWIRWTLPRFRYDQLMRLGWQYMIPLALIHLFATG
                     AVLLWADK"
     gene            complement(10962..11939)
                     /locus_tag="PK281_01260"
     CDS             complement(10962..11939)
                     /locus_tag="PK281_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018675835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOY47995.1"
                     /translation="MDLLKVTIDNQTIEVPPGTTILQAARMIGKEVAPPAMCYYSKLK
                     TSGGYCRTCIVKVSKGSEKDPRPMPKLVASCRTTVMDGMEVQNYTSPEVVDARKGVVE
                     FLLINHPLDCPVCDQAGECHLQDLSYENGKSKSRYEFERRTFERIDIGEKIQLHMNRC
                     IMCFRCVKVADQLTPGRVHGVINRGDVAEISTYIQNSIENEFSGNMVDVCPVGALTDK
                     TFRFKSRVWFLKPFDAHRNCDNEKCTGKAVIWKYGNAIYRVTGRKDQWGEVEDFICNT
                     CRYDKKELSDWTEEGPRMIDRHSVISANHYEKGTKYVASPESLPTKQLS"
     gene            complement(11956..13317)
                     /gene="nuoF"
                     /locus_tag="PK281_01265"
     CDS             complement(11956..13317)
                     /gene="nuoF"
                     /locus_tag="PK281_01265"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HOY47996.1"
                     /translation="MGRKLLLTHENVPGIQTYEVYRSKGGYASVEKAIKTMTPEAIVE
                     EVKTSGLRGRGGAGFPTGMKWSFLAKPEGVPRYLVCNADESEPGTFKDRYLMEHIPHL
                     LIEGMITSSFALGANTSFIYIRGEYFYVSRILENAIAEAYANGWLGKNILGSGYDLDL
                     YVQVGGGAYICGEETALLESLEGKRGNPRIKPPFPAIAGLYGCPTVVNNVETIAAVVP
                     IVNDGGEAYSKIGVGRSTGTKLISASGNINKPGVYEIELGLPVEEFIFSDEYCGGIAN
                     GKRMKACVPGGSSVPILPDNLVCKTAAGEPRLMTYESLSDGGFATGSMLGSGGFIVYD
                     ENQCIVRNTWNFSRFYHHESCGQCSPCREGTGWMEKVLHRLEKGLGNMDDIDLLVDVA
                     RRIEGNTICPLGDAAAWPVAAAVRHFRHEFEYHVNFPDKVKDPKHGQREPIMPVAPAP
                     VNA"
     gene            complement(13329..13910)
                     /gene="nuoE"
                     /locus_tag="PK281_01270"
     CDS             complement(13329..13910)
                     /gene="nuoE"
                     /locus_tag="PK281_01270"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HOY47997.1"
                     /translation="MSAHPTGTHSFDPALRSQLKFSEDAMATIQKIISRYPEGKSKSA
                     LLPILHIAQAENEGWLSPEAMDLVAQILGIQAIEVFEVASFYSMYNLQPVGKCHLEVC
                     RTSSCWSRGAEDIVDYISKKLNIKVGETTPDGMFTLKTVECLGSCGSAPMLQCGAEYH
                     ENLTEAKVDALLDRFRSENKRHNYTDLDYKNSK"
     gene            complement(13921..15132)
                     /locus_tag="PK281_01275"
     CDS             complement(13921..15132)
                     /locus_tag="PK281_01275"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018675843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HOY47998.1"
                     /translation="MAEQSIFGEENIQSENKQYNTLNLGPTHPATHGVFQNILQMDGE
                     IIMSSEATIGYIHRAFEKLAEHRPFYQVTPITDRLNYCSSPINNIGWHLTVEKLLGVE
                     TPKKVDYMRVIVMELSRIADHIVCNTVIGVDTGALTGFTYMFQERERIYDIFEQMCGA
                     RLTTNIGRIGGFERDFSAKTIQMIRDFLSGFPKRFDEFEGMLLRNRIFMDRIINVGPI
                     SGERALEYSFSGPNLRAAGVDYDVRVMNPYCSYNDFDFTIPVGTSGDTYDRFCVRQAE
                     IRESLKIIEAALNNMPEGKFVADCPEFYLPAKKDVYSSMEALIWHFKIVMGEVDLPKG
                     EVYHSVEGGNGEVGYYLISDGGRTPYRLHFRRPCFIYYQAYPEMIKGSMLSDAILTMS
                     SMNVIAGELDA"
     gene            complement(15143..15646)
                     /locus_tag="PK281_01280"
     CDS             complement(15143..15646)
                     /locus_tag="PK281_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HOY47999.1"
                     /translation="MALTKEITLSRLQDKFGDAILSHDEPYGLLTIEVPADRNLDVIG
                     FLKNESDLAFGYLTDLCAVHMPDQKGREIAVVYHLHSLTNNVRIRIKCWLPESNTEIA
                     SATGLYAGANWMERETYDFFGVRFLGHPNLKRILNVDHMEIFPMLKQYPLEDSSREDK
                     DDRFFGR"
     gene            complement(15648..16190)
                     /locus_tag="PK281_01285"
     CDS             complement(15648..16190)
                     /locus_tag="PK281_01285"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014083224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HOY48000.1"
                     /translation="MSDLKMVEAPAGVEGQGFFATSLDSVIGLARANSIWPLPFATSC
                     CGIEFMATMASNYDLARFGSERLSFSPRQADLLMVMGTIAKKMAPVLKHVYEQMAEPR
                     WVISVGACASSGGIFDTYSVLQGIDRVIPVDVYVPGCPPRPEQIIDGIMKIQELVKNE
                     SLRRRNQPEYKALLASYGIE"
     gene            complement(16212..16574)
                     /locus_tag="PK281_01290"
     CDS             complement(16212..16574)
                     /locus_tag="PK281_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004918335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HOY48001.1"
                     /translation="MNGTSEYIPLLIQLVFAAGFVLLVLVVTHLIGPKRKGDRKLESF
                     ECGIESKGNARLPFSIKYFLTAILFVLFDVEVIFFYPWATNFRDFGMDGFISMLIFVA
                     FFLLGFLYLWKKDAFKWE"
     gene            16819..17199
                     /locus_tag="PK281_01295"
     CDS             16819..17199
                     /locus_tag="PK281_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48002.1"
                     /translation="MMNDPDKSRFFADLRDLLVDYIQNRLDLTRAIAFEKIARIVAYL
                     VIGVILALLFFFGLLFLSFMLGFYLSDLLHSQLAGFAIVAGLYFLALAIVFVFREKLF
                     ARTIVDSIIRILYERQQTDEDEEA"
     gene            17186..17515
                     /locus_tag="PK281_01300"
     CDS             17186..17515
                     /locus_tag="PK281_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48003.1"
                     /translation="MKKRKIENLNDLHLEQSRLRMEVRNSEKAIGDRMEYLRRNYPSI
                     LLMQVLPFEDRKKDLIISGLGLAASLLMGRIAESGKELLEKNLQKLLQWVSKKFGGST
                     TEPQAED"
     gene            complement(17512..18870)
                     /locus_tag="PK281_01305"
     CDS             complement(17512..18870)
                     /locus_tag="PK281_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007094865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOY48004.1"
                     /translation="MYYKNILETIGNTPLVKINRITKDIPATVLAKIETTNPGNSIKD
                     RMALKMIEDAEKDGRLQPGGTIIEGTSGNTGMGLAIAAVIKGYKCIFTTTDKQSKEKV
                     DALRAFGAEVIVCPTNVEPEDPSSYYSVSSRLIAEVPNSWKPNQYDNLSNSQAHYEQT
                     GPEIWEQTEGKITHLVVGVGTGGTICGTGRYLKEKNPNIKILGIDTYGSVFKKYKETG
                     IFDKNEIYPYITEGIGEDFLPQNVDFNVIDHFEKVTDKDAAVMTRRIPREEGIFAGNS
                     AGSAMAGLLQMKDMFKEGDVVVVIFHDHGTRYLGKMFNDDWMRDRGFLEEKSPKAIDL
                     IERHKHLKLVTVDAEDSVGEAFAIMRKFDVSQIPVKSGAEFVGSLSDSHLYASICDNP
                     ELKQARVSDLMQKSFPFVNPQSKLEEVSKQINRENEAVLVRDMLGAVHIITKYDIIEA
                     LG"
     gene            18943..19749
                     /locus_tag="PK281_01310"
     CDS             18943..19749
                     /locus_tag="PK281_01310"
                     /inference="COORDINATES: protein motif:HMM:NF038402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HOY48005.1"
                     /translation="MIQPENTMITDMLGRTITLSKTPTRIVSVVPSQTELLYDLGLAE
                     EVVGITKYCIHPDDWFHSKTRVGGTKKINQEIILSLKPDIVIANMEENTQSELEELAK
                     HVPVWISDVRNLNDALRMIELLGDLVDKSEEAEKIILKIRSDFDLLKPISKPRKALYL
                     IWREPFMAINSDTFIHDMMMRCGLINVSSEAQTRYPQLSPEQVHALNPEYVLLSSEPF
                     PFSEKHIAEIQRLLPKARIGLVDGEAFSWYGSRLTQSAPYFKQLIRFWDK"
     gene            19845..22136
                     /locus_tag="PK281_01315"
     CDS             19845..22136
                     /locus_tag="PK281_01315"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOY48006.1"
                     /translation="MLILRILFSSFLLLVSTCVFGQKVGVVLSGGGSSGLAHVGVLKA
                     LEEAHIPIDFIAGTSMGALVGGMYAMGYSPAQIELLVKSEEFEGWVFGKPVDRYVYFF
                     REREPDANWVGIKFRIDSVIQTRFPTNIISPANMDFAFLERTSAISAAAGYNFDSLFV
                     PYRCVAADIYNKAEVVFKDGDIGQAMRASSTYPFFYKPIVVDDKLLFDGGLYNNFPAD
                     VMYRDFLPDVIIGSTVASEIRPPQEDDILSQIKNMLMDKTSYGEVCETDHMIIIKPLI
                     PKVAILDFNQTDAMIEGGYQSTLARLPQVKTMIYRRQDSLELANRRIAFKEKQKPVVI
                     DDINIEGLSKNQVNYIKRAFGEKAFPLPIENIRPYFYRVAYDERIKKIYPTARYDSKD
                     GHFLLNLKMKVDKDLQAQFGGLISSRPINTGFVALHLKRINRIGLALDANSYFGKFYG
                     SLQVKATLDYPFKLPFQVETDFTLNNFDYFYSSSTFFEDVKPSYLIQYDRSITGALVF
                     PWKNKSRIKIGLTGARLFDDYYQTQSFTTADTADRTNFNHGSFWMNFERNTLNRKMYA
                     NSGSQFFVKFRYLAGQEFNNPGSTSLQNIEYRRIHDFWRMKAVYDVYFNKRGRLHIGF
                     YGEAVVSNQPLFNNYTASLLQSAAFSPIPESRTLFQSQFRAHNYAAAGLKTVLSFTSR
                     LDLRLEGYIYQPHRVILENPDLTASYGDAFSKRYFMASAVLVFHSPIAPIAVSANFYD
                     QSKNPISILFTTGFLIFNRRAFD"
     gene            complement(22137..23003)
                     /locus_tag="PK281_01320"
     CDS             complement(22137..23003)
                     /locus_tag="PK281_01320"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOY48007.1"
                     /translation="MISLAPLSHYDQVTCFSLPGKPEMELTVRRFDKLHPEISGNKWF
                     KLIHWLKDLPAYEAVVSFGGAYSNHLLALSAAGRETGIRTIGIVRGDIVENPWLQAMK
                     QNGMEIHFVSRESYRQKTDHGFLQNWTDKLGSCRIVPEGGAGLPGILGAAEMVQSDEP
                     FDWIVLPGGTGTSAAGIAQKLEKSLSKVLCFQVLKGEQILQQELRKHGFDSSYYPNLV
                     FNADFHFGGYAKSKPELIAFQQQWIEQTGIPIDLVYGAKALFGLYALFLEQKLGNKKR
                     IIYIHTGGLGPN"
     gene            23076..23273
                     /locus_tag="PK281_01325"
     CDS             23076..23273
                     /locus_tag="PK281_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010518528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5522 domain-containing protein"
                     /protein_id="HOY48008.1"
                     /translation="MENEGFSRLSNLDKDEYYYSPEGYVVFTAKYLLKRGYCCKNGCR
                     HCPYNYNIRTGKFDKDAHGNS"
     gene            23260..25305
                     /locus_tag="PK281_01330"
     CDS             23260..25305
                     /locus_tag="PK281_01330"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007646469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HOY48009.1"
                     /translation="MATLSTTELEAFQHDLLAGIPTELPPTRKPNPAISHAPRRKDIL
                     SADEKKLALRNALRYFEPRHHAVLAPEFMEELKQYGRIYMYRFLPDFHMHARPVKHYP
                     AKCKQAAAIMLMIQNNLDHAVAQHPEELITYGGNGAVFQNWAQYRLTMQYLCQMEEDQ
                     TLVMYSGHPMGLFPSHKDAPRVVVTNGMMIPNYSKPDDWEKFNALGVTQYGQMTAGSY
                     MYIGPQGIVHGTTITVMNAARMCLRKRGDLQSDVIEGKGLLFVTAGLGGMSGAQPKAA
                     KIAGLVSITAEINPAAARKRHAQGWVDELHTDLDVLISRCEEAREKGEALSLAYEGNT
                     IDLWEALVRRGIRVDIGSDQTSLHNPFAGGYYPAGFSLEESNRMMADEPKRFHDEVYK
                     SLVRHAAAVNKLSDTGMYFFDYGNAFLLESSRAGADVKGATAEFRYPSYVQDILGPMC
                     FDYGFGPFRWVCCSGEAEDLDLTDQLAAEVLEELLQNAPDDIRGQIQDNLNWIRAAKK
                     NQLVVGSQARILYADSQGRIQIALRFNQAIADGTIRGPIVLGRDHHDVSGTDSPYRET
                     SNIYDGSRFTADMAVQNFAGDAFRGATWISLHNGGGVGWGEVINGGFGMVIDGTEDSA
                     RRIKSMLHWDVNNGIARRAWARNKEAVTAIKRAMELEPKLKITVASEVDNNIIDSLD"
     gene            25395..26561
                     /locus_tag="PK281_01335"
     CDS             25395..26561
                     /locus_tag="PK281_01335"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOY48010.1"
                     /translation="MIIRRAIHEIHELLEDFPVVAIIGPRQVGKSTLAKALEGDREIL
                     YIDLENPADRVRLGDPVLFFESNRDKLVVLDEIQFVPDLFPVIRSMADKYPENGRFLV
                     LGSASPDLLKQSSETLAGRIAYVRLYPFSYAELENEPDFTWQQLLLRGGFPKSYLARN
                     DSASLTWRNQFIQTFLQRDLATLGFNLNPAGMYNLWMMCAHYHGRILNHSSIGNSLGL
                     SNNTIRNHLEILKGTFMVDVLQPWLPNLSKRLVKSPKLYISDCGILNALLSVRNFSDL
                     LGNPIAGFAWEGLVLQTMRAQLPADWNIYYLSTAAQAEIDFIAIRGNQVLGIECKFST
                     SPALSRGAKELIKDLNLEEVIVVSPVENDFLMSTGVDVLSLKSFSARLVNVKYQ"
     gene            complement(26656..27621)
                     /locus_tag="PK281_01340"
     CDS             complement(26656..27621)
                     /locus_tag="PK281_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018676799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HOY48011.1"
                     /translation="MIRFFLFTLLALPVLASAQRKQTASEYIAAYKDIAITEMERYGI
                     PASIKLGQGLLESSAGNSDLATEANNHFGIKCKKDWTGPSFHKDDDAKDECFRKYETV
                     LASYEDHSQFLKNSTRYAELFTLEITDYKGWAHGLKKAGYATNPQYAQLLIKTIEDNR
                     LYEFDQKGGAVVESKPVRNRDKKPTQISNPRTPRGKSNETDLPDFELKKQGGRPIMVR
                     NRVQYVVAKQGDTFEALTTELDMLRWQLPKYNDLPPSAKLAAGDVIYIQPKRRKAFTD
                     SHTVKSGETMQSISQEHAIKLSRLYHLNQLEEGAQPSEGQQLKLR"
     gene            27770..>28439
                     /locus_tag="PK281_01345"
     CDS             27770..>28439
                     /locus_tag="PK281_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HOY48012.1"
                     /translation="MKLSQFKFKLPPELIAERPTDNRDESRLMVLNRKTKTIEHRMFR
                     DILEYFDDGDTFIINNTRVFPARMYGNKEKTGAKIEVFLLRELNAESRLWDVLVDPAR
                     KIRIGNKLYFGDDDSLVAEVIDNTTSRGRTLRFLFDGPHEEFKKVLYSLGETPIPKYI
                     NRKPDEEDELRFQTVYAKNEGAVAAPTAGLHFSRELMKRMELKGVNFAEITLHVGLGS
                     FRTVE"
BASE COUNT         8339 a         5925 c         6183 g         7892 t
ORIGIN      
        1 cctatgcatt ttcgatgtga tgatgccgca aatggacggt tttactctcg ctaaagagat
       61 tcgaaatcac aataaagaca ttcccattat tttcctcact gcgaaatcga tgaaggaaga
      121 taccatcgaa ggatttaaag ccggaggaga tgattacatg actaagccat ttagtatgga
      181 agaactcttg cttcgcataa aagcaatcct caaacgtacc gaacgcgtgg tggatgatgg
      241 tcctgttcaa gaattcatca ttgggaaata cagtttcaat ccaacgcatc agcaactaac
      301 attaggtgac cacattcaga aacttacttc gcgtgaatcg gatttgctta agttgttgtg
      361 tatgaatttg aacgacactt tagagcgaaa ctttgccctt cgtgcgattt ggaatgatga
      421 taattacttc aatgctcgaa gcatggatgt ttacattgct cgtttgcgta aatacctaac
      481 tggtgatcca gcactcgaaa tcatcaatgt tcacgggaaa ggattcaaac tggtagacgg
      541 acatactcca tcatcctagt taactcgaca tcttgaagtt tgccagcttt ttatagttgc
      601 gcaaaatggg aatcatccaa aagccaacaa atacggcaca taccgcaaac gcataaacaa
      661 tttgatcgtt ccgattgaaa aatgggattg cgaaaagaga gctgattccc gctcgaaaca
      721 agatgtttat ggtttgaaac acgctgctta cccgaccaat cgattgattc ggcacatgct
      781 gaaacaacca agttgttcgt aatacgcgcg ttccagcatt ggtaattcct accaataagc
      841 aaaggataga aaataatagt aagttagaac tgctcgcaaa aagcgcataa attagcactg
      901 taatgcccat cattactgta attccgagta taaaaccaga ttttcggaat gtccagacca
      961 taaaaacacc tgctagcata gctcctatcg agtacatgac ttccgcaaac gcataagtac
     1021 cagcgcctga atgtaaatgc tgatcaatgt attgaggaag taattgctga aatgtaacca
     1081 gcaaaacaac gaagattgcg taggatgcat ttccaaactg gaaaatcagt ggattgtctt
     1141 ttagaaattt catacccgtt cgaattcgat cgagcgcaga accagcttct tgatgacgaa
     1201 tggcaatcgc ttcataacga atcaaggcga ttagaaagaa acctattaga taagtaatcg
     1261 catccgtcag gaaaacttct tgcaaagtcc aaggcttaat ttcaaaagga aagttgatcg
     1321 ttaagccggc aaaatgatgt tctccctctg aaacacctga tagtaaaagc gccgcagctg
     1381 atccagataa gatactcgtt gactgtccta tgatttctgt ccaggaagtg aatcgaccgt
     1441 aatattgctt ctcggtaatt tcttgtccga atgcatacaa ggctggataa tgaatgttat
     1501 acacgaggat ggttccagca aatacgagca ttgctcccca agctggcaca gagccttgat
     1561 aaaatccgat tgctgaaaca gagcctagta atagcaaacc aaaagcattg acagccataa
     1621 atacatggcg ccgagagaat cgatcgatca gagagccggc atacaatccc caaaataaca
     1681 tgatgaatgt ggtggctgca tacatcatcc caaaaagctc gggcttacct agaattttac
     1741 tgaagtacca aggaatagct aacatgcata ttccctgcga caggcccgat acagcattgg
     1801 caataaaaag caagcgataa gccctagcgt tcttcacgga gcaaagatag aaaggcaatt
     1861 gggggtaatt ggatgggcgc aaaatctttc atccacaatg cttgtgaaat aaatggcgct
     1921 tactacctat tgcttgcagg tatattccat ctatcttgtt ttcgaaataa acggagagat
     1981 tcgcatgagt gcacacaaaa aattatcgct gttcgacagt tcaaccatca tcatgggttc
     2041 gatgattggc tcgggaatat ttatcgtaag tgccgatatc gctcgccagg taaattcnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaag aatattctgc tcgatttagg
     2221 ttttctaact gtaagcacgc agcaattgac cgccattttg gtgattgttt ttctttcctt
     2281 gttgaatctt cgggatgtga aaaattcagc aatcattcaa aatttcttca cggttgcaaa
     2341 gattgccgca ctgttgggtg ttgttgctgt aggaatttac ttcgcaggac agggaaaagg
     2401 aaactggcaa aatatggaac ccgtttggcc cgagaacatg ggcttaggaa caatcgggat
     2461 attcttggct gctttaactg gaagtctgtt ttcggctgat gcgtggaata atatcacgtt
     2521 tacagcaggt gaaatcaaaa atcctggaaa aaacctaccc ctttccttag ctatcgggac
     2581 tggtattgta ttgctgattt acatcttggt aaatgtgatt tacctgtata tgatgccttt
     2641 agcggacatt caacatgcac ccgaagatcg agttgggact gcgttgatga attccatgtt
     2701 gggaagtaat ggacagtttg taatggctgc tatcattatg gtttcgacat ttgggtgttt
     2761 gaacgggttg atattcactg caccacgagc atattatgcc atggcgaagg atggattgtt
     2821 tttgaaacgc gctacacaac tgaacaagca tggtgtaccg gcatttgcta ttttaataca
     2881 gctggtttgg agcagtgcgt tgtgcctatc gggcaagtat ggcgatttgc tcgactattt
     2941 gatgtttgtg gtgatgttgt tttacatttt aacggtagga gctgtgttta ttctgcgacg
     3001 aaaaatgcct gatgcacctc gtccttacaa agctattggc tatccgatta ttcccatttt
     3061 atacattctt ttggcgactt gtatttgtgg tgtgatgctt tgcttcaaga ccactttcgc
     3121 tttgtatgga ctggcaatcg taggtgttgg aattattgtc tatcagttta gttccaagaa
     3181 agaaatcgaa gacaagactg ctcaataaaa tgcctgtaat ttttactgtt accgaagctg
     3241 ttgagaaaat gaaaaagttc tgtgcttatc aggagcgcag tcagcaagag gtgcggcaga
     3301 aattgtatca aagctccatc aaaggcgaag aagccgaatt tgtgattggg gaattaattc
     3361 gggagaattt cctaaacgaa gaacgttttg ctcgtgcatt tgcccgaggg aaattccgga
     3421 tgaaacaatg gggaaagatt cggattcgca tggaactgca acacaaaggc gtgtcggaac
     3481 cctgcattcg aaaaggcttg tcggaaatca gcgatcgcga ttatcgtaaa acagccgaag
     3541 ttgtattgaa gaaactttcg ggcggagtac ttccagattc taaaaaagaa gcatggaaac
     3601 tcctgagtaa aatgcgttca aaaggcttcg agcctgaatt acttcagtca atcatgaatg
     3661 atttctccga agaataaaga ttaattacct ggaagattag ctagcaaatc agggaagatt
     3721 cccaacaaga gcgtaaggaa aacacccaat gccagcacaa aattgaaggt ccagctagtt
     3781 tgaatacgct ccattggttg tccatcgttg ttgaatgcaa aaatcactgg acggaaatag
     3841 tagtaaatac caatcagcga acctgcaata gcaatgatga ctgcaatgtt caggttgctt
     3901 tccatcgctg ttaggaaaac gaaatacttt ccgaagaaac ctgccaatgg aggaatacct
     3961 gccaaggaga acatggaaac aacaagagca agggtcatca tcggattgtt cttagccaag
     4021 cctttgtagt tatctaccgg catttcgtta ttaaactgac taatcgcgat tagcacaccg
     4081 aaagcagtta cagaagctac cgagtaagca gcagtgtaat agaaaatcga agaagaagaa
     4141 agctcattca acgctaaaac aggcaataat aaatatcctg catgagcaac cgaagaccaa
     4201 gcaagcatac gtttcgcttc gctctgcata acagcagaaa tgttcccaag taaaatggtc
     4261 gcaacagcaa tcacccaaag gatattgtac cacacgccat caattccgct gaaacacgta
     4321 tcgaacaaac gataaaatgc tgcaatacca gccgttttaa ccacagtagc catgaaagct
     4381 gtgataagcg ttggagctcc ttgataaaca tctggagtcc agaaatggaa cggaactgca
     4441 ccaattttaa aggcgaaacc aattagaatc aagacaactc ctgtgttgaa aatcaaagga
     4501 agattgtcgg tgccgttagc gtaatcggag attgctgcta aatcgaaact tccggtaaca
     4561 ccatacatca aagtaattcc aaacaataag aatccggatg cgaatgctcc catgaggaag
     4621 tatttcaaag aagcttcatt agaggcaaga ttcaatcgac gacttcctgc caagatatac
     4681 aaggcaatgg aaaggatttc gatacctacg aatagcatga ccaaacttga atacgaagtc
     4741 atgactaaag cacctaccag tacaaatatc atcaacgcat aaatatcggc cacatggctg
     4801 tcttcgttac gaaagtgata gctggcaata agcagaatca agatgagtgt tacgatcatc
     4861 aatccggtaa atgaaaccgc atagttatcg tacagcatca tgttatacca aaggtgattg
     4921 gttccccaat caagcgcatt ggtaataaat gctgccacta agcctaaaat ggctaccggc
     4981 agaagcaatt ttttctgatg aaaggctcca agaaataaaa cgaggaagcc aagaagcgac
     5041 aggagtacca gtgtagacat atcagagatt cggtaccatg tgattagaaa tctcggtgag
     5101 taagtacttt accgactcat ctgtgagatt gaaaataaac tgagggaaca atccgaagaa
     5161 aacgatgagt ccaacgattg ggaacaacac cagtttttcg gaaatggtaa ggtcggcaaa
     5221 caccgtgatt gcaggattct caccaagcat cgatgttttg taagcacgta acatataaac
     5281 agctccaaaa atgacggcta aaccagcaaa tgcggcaaac acataactgt aatcgtacaa
     5341 agagctaagc agcaagaatt ctccaataaa tccattggtt agcggtaaag ccacactagc
     5401 aaaggttatc accataaagc aggaagcaaa cactggagct aactgacgaa taccgcccaa
     5461 cttggcgatt tcgtttgtcc cagtccgctg ttgaatgatt tcggctacga agaacaatcc
     5521 aactacgttg acaccatgtg ccagcatttg aatcattgcc ccttggaaac ctgtgcgtga
     5581 aagtgtgaaa atacctgctg ttattagtcc aacgtgagca atcgacgacc aagcaaacaa
     5641 acgcttaaga tctttttgcc cgatggcaat ccaagaagcg tagattattc ctaccacaca
     5701 catgattaaa accacatcgg tgtaggaact aacagccatt ggtgtgatag gtagaatcca
     5761 acggaagatg gagtaaatac ccatttttaa catgatacct gatagtaaca tcgaaccagc
     5821 attgggtgcg gtagtatagg tatcgggctg ccaagagtga aatggtagta aaggaatttt
     5881 gatcgcgtag gcgatgaaga atgcccagaa aatccaacgt tgagcaactg gatcaaggcg
     5941 taatgcatag aaagcatcaa ttccaaagct acgatcggtt gtttgaagat acagccagat
     6001 aattgccgcc aacatgagta gcgatccaag taaggtgtag ataaagaatt tgaaggtgat
     6061 tttcacccga ttttctccac cccaaatgag gacgatgaaa tagattggaa taagcgctaa
     6121 ttcccagaag atgtagtaaa ggaaggcgtc taatgatacg aacacgccaa ctagtgcact
     6181 ttgcatcatc aacatgagga aatagaaaag gcgcggattg cggaattctc ggttccaagt
     6241 tgtgaggacg ataagtggta gaaggaaatt ggttaaaata accatgagca tacttagtcc
     6301 gtcgagcccg acgaagaagc gaattcccat ggtagagatc cagttttcgg acagaataaa
     6361 ctgttcgtta gaaagcaagc ttcggtgttc gttgatgtcg aacaggaaga tgacataggc
     6421 cgtaaaggct gcctgaataa gggtggcaac gagggcgcct ttttttgccg catttgcagg
     6481 aagcaaaaac agcattaggg cagccaggaa cggaaacgcg atgagggcag ctgttatcat
     6541 gctcagttca gaaagctgtt agccgcgagg atgaccaaaa ctccgatcac cattgcgaaa
     6601 atgtaaaaac cgacattccc cgtttgtaag aaccgagctg tgcgggaacc caacacaacg
     6661 agttgtccaa caccgttcac aatgcgatcg atgacgttta cttcgaggaa tttatatatg
     6721 ccttttgatg cagaaactac cggattagct acgacagagt cataaagctc atcgatgtag
     6781 tatttattca cgagtagctt ctcaaatgcc gtaatgtgtt cgccttcggc agcaggaata
     6841 tgtccgttct ttttataccg aatccaagca tacccaataa caccaagaac tgcagaaaca
     6901 gatatcacca ttaagatgat ttcgagcgaa tgagaaagat gatgctctac accaaaatct
     6961 tccaaaataa gagatgatcc agctaaaatt ggttcgaaga aacgatctaa ccaatgtgtg
     7021 ccacccaata cttcagggat attgacaaat ccagcaaggc aagaacagat ggctaaaatc
     7081 accaaaggaa tggtcatcgt tttaggcgac tcatgcaggt gatgttcttg ttcgtgcgtt
     7141 ccgcggaatg aaccaaagaa tgtgaggtaa tacaaacgga acatgtagaa tgcagtcatc
     7201 aatgctccac aaacacctag aatccatagc actggattgt gtgcaaatgc agaagccaag
     7261 atttggtctt tcgaaaagaa tccggcaaga ggaggaatac cagcaatggc gatagttcca
     7321 atgaggaagg tgaaatgtgt tattggaaga gcctttttca atccgcccat tttgcgaatg
     7381 tcttgttcgc cgcccattgc gtgaataacc gaaccagatc caaggaacaa caaggcttta
     7441 aaaaaggcgt gggtaaacaa atggaaatag gcagcagaat aagctcctaa tccaagtgcg
     7501 aaaaacatat aacccaattg tgaaaccgtt gaataggcta gcactttctt gatgtcgttc
     7561 tgcttaaggc cgatactcgc agcaaaaagc gccgtgcata agccaacaat catgatgatt
     7621 tcttgcgtga atggtgctaa cgtaaatagc acattgctac gaataatcaa gtaaacacct
     7681 gctgttacca tggttgcagc gtggatgagt gctgaaacag gcgttggacc agccatcgca
     7741 tccggaagcc aagtgaataa tggcaattga gcagatttac caatagctcc tacaaacaat
     7801 aataatgttg cgttgcggat aattggatca cccgacataa tatctaaagc ttcaagcgaa
     7861 atgcggttgt attctaaagt agaaaaacgc agatacagta agaagatacc taatagcaat
     7921 cccaagtcac caatacggtt catgatgaaa gccttgttcg cagctttgtt gtattcgggg
     7981 tttttatacc agaaaccgat gagtaagtag gaacacaatc caactccttc ccaaccgatg
     8041 aatagcatga tgtagttgct tcccatcacc aaaatcaaca tgaagaatat gaagagattg
     8101 aggtaggcaa agaatcggct aaaacctgga tcgtcatgca tgtatccagc agaatacacg
     8161 tgtatgagga atccaactcc ggtaataacc agcatcatca ttaaagagag ctgatcgagt
     8221 tggaagctgt agttaacaga tagttttccg atttgaatcc attcacctag caggatgcgg
     8281 ataggctctg cgtcaagtgt attgacaaac ggaatgaaga tcgatacagt aagtatgaat
     8341 ggcactaaaa cggccacaga agcaacagat gctacaagat tcttcggaag acgttttcca
     8401 aagaagatgc tgaacaggaa tccgatcagc gggagtgcgg gaatcaacca ggctaagtct
     8461 ttcatattca agcgtgctgc cggtgcgtta ccatttcaag tttctcaatg cattaatgtc
     8521 ggtcgatcgg atgttccgat atatcattac caagatgcct aaaccaacag caacttcggc
     8581 tgcggcaacc accatgataa agaagacaaa cacttgtccc gactgatctc cgtgaaaacg
     8641 agagaaagca gcaaggagaa tatttacagc attcaacatg agctcaatag acatgaagat
     8701 gatgataaag tttcggcgga gaagaacgcc catcacaccg atcgagaata gaatcgcgct
     8761 caggaagagg tagtgatgaa tgccgatttc gcggaaggag ttctgtagtt cttccatgat
     8821 cagtcgttaa cgtgtttttt acccaaaata actgcaccta ccatggctga gatgaacagg
     8881 atagaagcta cttcgaaagg aaggagaaaa tctttgaaca gcactttacc gacacctttt
     8941 gctgtaccaa attcttcggt aatcattgtt gcaggtcgag tgtcgtattc acgaagtgat
     9001 gcagccaaaa taatcatgag caagcatgct gaaaagacag ctgccacttt aagcaacatg
     9061 ggtttatgtg gctctgtttc tttattgaga ttgagcaaca tgattacgaa aaggaagagc
     9121 accatgatgg ctcctgcata aacgatcaag ttgacaatcc cgagaaatcc ggcgtcaagc
     9181 aggatgtaat gtccggtgat ggaaaagaaa cagatcacca gccaaagcac gctgtagatc
     9241 ggattcttgg agaataccac catcagcgcg gagccaatgg taagtgctgc aagaataagg
     9301 aaggcgatcg gtgtcatagc ttagtatcgg gcgcgaaaat agcggaattt tatgatttag
     9361 gggaggaatg attagcggtc ctggcgtttc gtgatatcaa ttcgtttaga agcgtcaaca
     9421 ccttctacca aacggtcttt tccaaagatg aaatcatccc gatcataatc agatggaaca
     9481 atctctttgg ttaggaaaat ggcttgtttc gggcaggctt cttcacacaa tccacagaaa
     9541 atacaacgca acatgttgat ttcataaatc gctgcgtatt tctcttcgca gtaaagtttc
     9601 tcttcgcctt tcttgcgttc atccgcaacc atcgtaatgg cttctgccgg gcaagaaact
     9661 gcacataaac cacaagcagt gcaacgttca gctccatttt catcgcgctt caacacgtgt
     9721 tttccacgat aaacttcgct gaattcacgt gtttgttcgg gatatttaat ggtggcagga
     9781 cgtttaaaaa agtgtttcag tgtaaccgcc attcccttta aaaccgcagg taggtagcta
     9841 ccttcggcta gggtcatgtt tttatcggat acgactttcg atcggttggt gagttgcatg
     9901 tttttctgtc ttcagaaata aatgaacagt cttatttatc agcccaaagc aaaactgctc
     9961 cggtagcaaa taggtgaatc aaagcgagtg gaatcatgta ttgccaaccc aaacgcatca
    10021 attgatcgta acggaaacga ggtaaagtcc agcgaatcca catgaacaag aagatgaatg
    10081 cgaaaatttt gaggaagaac acgaatacac ctattatggt gatgatgttt tgatctaatc
    10141 ccaagctatc catgaacggg aagttatatc ctccaaagta caagctagcc atgaacgccg
    10201 atgaaatgaa catgttgatg tactcagcaa acatgaagaa tcccaatttc atggatgaat
    10261 attcggtgtg atatccacct accaattccg tttcacattc aggtaaatcg aaaggagtac
    10321 gattacattc tgcaaaagca cacaccacaa aaagcaagaa accgaatggt tggtagaaga
    10381 tgttccaatt cattccgtgt tgttgatcta caatttcctt aagacttaaa gtcccagtca
    10441 tcataatcaa ggcaatcaac gccaagccca tactcaactc gtagctgatc atttgcgaag
    10501 aagcacgaat agctccgagt agcgcgaact tattattgga agcccatccg ccaatcatga
    10561 ttccataaac ccctaaggat acaacaccaa aaatgtagag tacaccaatg tttaaatctg
    10621 aaacttgaag cgaaatttca cgtccggcaa tgttcaatgt atcaccccaa ggaataacaa
    10681 cactggtcat taatgccgtt aacatggcga ttccaggacc tgcgatgaac aagaatttgt
    10741 cggaagcatt cgggataatt tcctctttga agaagaattt acccgcatca gctaatggtt
    10801 gaaggattcc gaatggcccc gcacgatcag gtcctacacg gtcttgcatg aaagctgcaa
    10861 ttttacgttc agcataagtg ctgtaagtag cgatgagcag cgtgatggca aaagcgataa
    10921 acgcaatgac agttttatcg agtaaaaaca gcatgtccat atcagctcag ttgtttggtt
    10981 ggaagggatt ccggagaagc cacatactta gttccttttt catagtgatt ggccgaaata
    11041 accgaatgac gatcaatcat gcgcggtcct tcttctgtcc aatcgcttag ttctttctta
    11101 tcgtaacgac aggtgttgca gatgaaatct tccacttctc cccattggtc tttacgtccc
    11161 gttacccggt aaatagcgtt gccatatttc cagataactg ctttaccggt gcatttttca
    11221 ttgtcgcaat tgcggtgcgc atcaaatggt ttaaggaacc acacacgact tttaaaacgg
    11281 aaagttttat cagtaagcgc tcctaccgga cacacatcca ccatattacc ggagaattcg
    11341 ttttcgatgg aattttggat gtaagtactg atttcagcca catcacctcg gttgattaca
    11401 ccatgaacgc gacccggagt gagttgatcg gccactttca cacaacggaa gcacataatg
    11461 caacggttca tgtgaagttg gattttctca ccaatatcaa tacgttcaaa cgtacgacgc
    11521 tcaaactcgt aacgactttt actttttccg ttttcgtagg aaagatcttg caagtgacat
    11581 tcgccagctt gatcgcaaac agggcaatcg agcgggtgat taatcaatag aaattcaaca
    11641 acacctttac gtgcatcaac tacttcaggt gaagtataat tctgaacttc cattccgtcc
    11701 attacagtgg tacggcaaga agcaaccagt tttggcatcg gacgcggatc tttttccgag
    11761 cctttgctca cttttacaat acaggtacgg cagtatcctc ccgaagtttt tagcttcgaa
    11821 taatagcaca tagcaggcgg cgccacttct ttcccaatca tgcgggcagc ctgaagaatc
    11881 gttgttcccg gaggaacctc gatggtttga ttgtcgatcg ttactttcag caagtccata
    11941 gatacatgcg tcaaattaag cgttgaccgg agccggagct acgggcataa taggttcacg
    12001 ttgtccgtgt ttcggatctt tcactttatc agggaaattc acgtggtatt cgaattcgtg
    12061 gcggaagtga cgaacggcag cagcaacagg ccaagcagca gcatcaccca gcggacaaat
    12121 ggtatttcct tcgattcggc gagctacatc aaccaaaagg tcgatatcgt ccatgttgcc
    12181 taaacctttt tccaagcggt gaagcacttt ttccatccaa cccgttcctt cgcggcaagg
    12241 gctgcactgt ccacaacttt cgtggtggta gaaacggctg aagttccacg tgttacgaac
    12301 gatacattga ttttcgtcgt acacgataaa tcctccagaa cccaacatcg aacctgttgc
    12361 gaaaccacca tccgaaagtg attcgtaggt cattaagcga ggttcgccag ctgctgtttt
    12421 gcacaccagg ttatcgggta gaattggaac agatgatccg cccggaacac atgctttcat
    12481 gcgttttcca ttggcaatac ctccgcagta ttcgtcagag aaaataaatt cttctacagg
    12541 taaaccgagt tcaatttcgt aaacgcccgg tttgttaatg tttccagaag cagaaatcaa
    12601 tttagttccg gttgaacgac caactccaat tttcgaatag gcttctcccc catcgttcac
    12661 aattggaaca acggcagcaa tggtttctac gttatttaca actgttggac aaccatacaa
    12721 tccagcaata gcgggaaaag gaggtttgat acgcggattt ccacgtttcc cttcgagtga
    12781 ttcgagtaat gcggtttctt caccacagat gtatgctccg ccacctactt gaacgtataa
    12841 gtcgaggtcg taacctgaac ccaaaatgtt ttttcctagc cagccattcg catatgcttc
    12901 ggcgatggcg ttttcgagga tgcgagaaac atagaaatat tctccgcgga tatagataaa
    12961 tgaggtgttt gcacctaaag cgaagctaga agtgatcatt ccttcgatga gcaaatgagg
    13021 aatatgctcc atgaggtaac gatccttgaa tgttcccggt tcggattcgt cagcattgca
    13081 aacgaggtaa cgaggaacac cttcgggttt agcgaggaag ctccatttca ttcctgtagg
    13141 gaaaccagca ccaccgcggc cgcgtaatcc agaagttttc acttcttcta cgatggcttc
    13201 aggagtcatg gttttgatgg ctttctccac cgaagcatat ccgcctttgg agcgatatac
    13261 ctcataggtt tgaatacctg gtacgttttc gtgtgttaac agcagttttc tacccatgat
    13321 gcgcgctatt acttactgtt tttgtaatca agatcggtgt agttgtggcg cttgttttcg
    13381 cttctaaaac gatcgagtaa agcatccact tttgcttcgg tgagattttc gtggtattcg
    13441 gcaccacact gaagcatcgg tgcacttcca caagaaccta aacattccac agttttaagt
    13501 gtgaacatgc catcgggtgt ggtttctcca acttttatgt tgagtttttt gctgatgtaa
    13561 tcaacgatgt cttccgcccc gcgtgaccaa caagaagaag tacgacatac ttcgaggtga
    13621 cattttccta ctggttgtag gttgtacatc gaatagaacg aagcaacttc aaacacttca
    13681 atggcttgaa ttcctagaat ttgagcaacc aaatccattg cctcaggact taaccaacct
    13741 tcattctctg cttgtgcaat atgaagaata ggtaataagg ctgatttgct tttcccttcg
    13801 ggatagcgac tgatgatttt ctgaatagta gccatcgcgt cctcgctgaa tttcagctga
    13861 gagcggaggg ctgggtcgaa ggaatgcgtt cctgttggat gtgctgacat tcgataaatt
    13921 ttaagcgtcg agttcgccgg caatgacgtt catactcgac atagtaagga tggcatcgga
    13981 aagcatcgaa cctttgatca tttccgggta agcctgatag taaatgaaac aaggacggcg
    14041 gaagtgaagt cgatatggag ttcggccacc atcagaaatg aggtaataac caacttctcc
    14101 atttcctcct tcaaccgaat ggtacacttc ccccttagga agatctactt cgcccatcac
    14161 aattttgaaa tgccaaatga gtgcttccat gctggaatac acgtctttct tagcaggaag
    14221 atagaattcg gggcagtcgg caacaaattt cccttcgggc atattgttca acgcagcctc
    14281 gataattttc aagctttcgc ggatttcggc ttgacgcaca caaaaacgat cgtatgtgtc
    14341 tccactcgtt ccaaccggaa tggtgaaatc gaaatcgttg tatgagcaat atggattcat
    14401 cacccgcaca tcataatcaa cgccagctgc acgtaaattc ggaccgctga agctgtattc
    14461 taaagcacgt tcacccgaaa taggaccaac attgataata cgatccatga aaatccggtt
    14521 gcgtaaaagc atcccttcaa attcatcgaa acgtttcgga aaaccagaaa ggaaatcacg
    14581 aatcatttga atggtcttcg cactgaaatc gcgttcgaaa ccaccaatac gaccaatgtt
    14641 tgttgttaaa cgtgcaccac acatttgctc gaaaatatcg tagatgcgtt cgcgctcttg
    14701 gaacatgtaa gtgaagcctg ttaaagctcc agtatcaaca ccaattacgg tgttgcaaac
    14761 gatgtgatcg gcgatacgtg aaagttccat cacaatcaca cgcatgtaat caactttctt
    14821 tggcgtttct acaccaagaa gtttttctac agtcaagtgc caacctatgt tattgatcgg
    14881 tgaagagcaa tagttaaggc ggtcggtaat aggagttact tgataaaaag ggcggtgctc
    14941 ggccagtttt tcaaatgcac gatggatata gccaatagta gcttcggagc tcatgatgat
    15001 ctctccgtcc atttgcaaaa tattctgaaa aactccgtga gttgccgggt gagtaggtcc
    15061 gagattaagc gtgttgtatt gcttattctc cgattggatg ttctcttcgc cgaaaatcga
    15121 ttgttctgcc atgaatttcg tgttaacgtc caaagaaacg gtcgtcttta tcttcgcggg
    15181 aagaatcttc cagcgggtat tgtttcaaca tggggaaaat ctccatgtga tctacattga
    15241 gaatgcgctt taggttcggg tgaccgagga aacgaacacc aaagaaatcg tatgtttcgc
    15301 gctccatcca attagctcca gcatataatc cagtagccga agcgatttca gtattggatt
    15361 caggaagcca gcatttgatg cggatgcgaa cgttgttggt taggctgtgc aggtgatata
    15421 ccactgcgat ttcacgtcct ttttgatcag gcatgtgaac agcacataaa tcggtgaggt
    15481 acccgaaagc taaatcggac tcatttttca gaaaaccgat tacatccaga ttacgatcag
    15541 caggcacttc gatggtaagt aaaccataag gctcgtcgtg actgagaatc gcgtctccaa
    15601 atttatcctg aagtcgactc agggttattt ctttggtcag tgccatatta ttcgatgccg
    15661 taagaggcaa gtaaagcttt gtattcgggt tgattgcgac gacgcaggct ttcgtttttc
    15721 acgagttcct gaattttcat gataccatcg atgatttgtt ctggacgagg agggcatcct
    15781 ggtacatata catcaaccgg aattactcgg tcgatacctt gaagaacgct atatgtatcg
    15841 aaaattccac cacttgaggc acaagctcca actgaaatca cccaacgtgg ttcagccatt
    15901 tgctcgtaaa cgtgttttag aactggggcc atttttttag caatggttcc catcaccatc
    15961 aataaatcgg cttggcgagg agagaaactc aaacgctctg atccaaaacg agctaaatcg
    16021 taattggaag ccatggtagc catgaactca attccacaac aagaagttgc gaagggcaaa
    16081 ggccaaatcg aatttgcacg agcaagacct ataaccgaat ccagactagt agcaaagaaa
    16141 ccttgtcctt ctacacctgc tggtgcttcc accatcttca aatcactcat attgctttgt
    16201 gtattgttgg attattccca tttgaaagcg tctttcttcc atagatatag aaaaccaagt
    16261 aggaagaaag caacaaaaat tagcatgctt ataaagccat ccattccgaa atcgcggaaa
    16321 ttggttgccc atggatagaa aaagatcacc tctacatcga acaaaacgaa aagaatagcg
    16381 gtgaggaaat acttaatgga gaaaggcaaa cgtgcgtttc ctttagattc gataccgcac
    16441 tcaaagcttt cgagtttgcg atctccttta cgttttggac cgataaggtg agtaactacg
    16501 agcaccaata gtacaaagcc cgcggcaaaa accaattgta tcagtagcgg gatgtattcg
    16561 cttgttccgt tcatgagcgt tagttttggc gaatagatgt aacacagttt taatgaatgt
    16621 gttcagcgat tccgcccgaa ttttgcgccc aaatgtacaa acatttgtat ttggaaatga
    16681 ggtagaatga ttacaaataa atcccttctt aattgtggat tctcaatcct gtaaaattca
    16741 accgaaagtc acgattctaa taacatacat tgaaacttct caaacgcatc aaatccatta
    16801 atataatacc tttatagcat gatgaatgac ccagacaaat cccgattttt cgccgatttg
    16861 cgcgatttat tggttgacta tattcaaaac cgactcgatt taacacgagc aattgccttt
    16921 gaaaaaattg cccgaatcgt tgcctactta gtgattggag ttattctagc gctccttttc
    16981 ttttttggct tgttgttctt gagctttatg ttaggattct acctaagcga tttgcttcac
    17041 agtcagctag caggttttgc gattgtggca ggattatatt tcctagcatt ggcaatcgtt
    17101 ttcgtattca gagaaaagtt atttgcccgc acgattgtcg attcgataat ccggatttta
    17161 tatgaacgtc agcaaacgga tgaagatgaa gaagcgtaaa atcgaaaacc tcaacgattt
    17221 gcatttggag caatcacgtt tacgaatgga agtgcgtaat tccgaaaaag caattggcga
    17281 tcggatggag tatttaagac gaaactatcc ttccattttg ctcatgcaag tcttgccatt
    17341 tgaggacaga aaaaaagacc taattataag cgggttggga cttgcagcga gcttgttgat
    17401 gggacgaatt gccgaatcag gaaaagagct cctggaaaag aatctccaga agctcctaca
    17461 atgggtaagt aaaaaatttg gtggttcaac aactgaacct caagccgagg attaacctag
    17521 tgcttcgatg atatcgtact tggtaataat atgaactgcg ccgagcatat cacgcactaa
    17581 aacagcttcg ttttcgcggt taatttgctt cgatacttcc tccagtttag attgaggatt
    17641 tacgaatgga aatgatttct gcattaaatc acttacacgc gcttgcttca attcgggatt
    17701 atcgcaaata cttgcatata agtgactgtc gcttaaagaa cccacgaact cggctcccga
    17761 tttcacagga atttgagaca cgtcaaattt gcgcatgata gcaaaggctt cacctactga
    17821 atcttcagca tcaactgtga ctaatttcaa atgtttatgg cgttcaatca ggtcgattgc
    17881 tttcggagat ttctcctcca agaaaccgcg atcacgcatc caatcgtcgt tgaacatttt
    17941 gcctaagtag cgtgtaccat gatcgtggaa aataaccaca accacatcgc cttccttaaa
    18001 catatctttc atctgaagta atcctgccat agctgaacca gccgagtttc ctgcgaaaat
    18061 tccttcttca cgcggaatac gacgagtcat caccgctgca tctttatcgg ttactttttc
    18121 aaaatggtcg atcacattaa aatcgacatt ctgtggtaga aaatcttccc caattccttc
    18181 ggtgatgtat ggatagatct cgtttttatc aaaaatgccg gtttctttat atttcttgaa
    18241 aaccgaacca tacgtatcga tacccagaat tttgatgttc gggtttttct ctttcaggta
    18301 gcgacctgtt ccacaaatag ttccgccagt tccaacacca acaactaaat gcgtgatttt
    18361 tccttcggtt tgctcccaga tttcgggacc ggtttgctcg taatgtgctt gagaattact
    18421 aaggttgtcg tactgattag gcttccaaga atttggaact tctgcaataa gtcgactaga
    18481 aacagaatag tatgaacttg gatcttccgg ttctacgttg gttggacaaa caattacttc
    18541 cgctccaaaa gcgcgtaaag catccacttt ttctttggat tgtttgtcgg tagtggtgaa
    18601 gatgcatttg tatcctttga taacagcagc aatggcgaga cccattccgg tgtttccgct
    18661 tgtgccttcg attatggttc cgccaggttg aagtcggcca tctttttcgg catcctcaat
    18721 catcttcaag gccatacgat ccttgatgga attgcccgga ttggttgttt cgattttagc
    18781 gagaacggta gccggaatat ctttagtaat tcgattaatc tttaccaaag gcgtattgcc
    18841 gatagtttcg aggatgtttt tgtaatacat gcggcaaagg tagaaattac ggaaattgcg
    18901 gaactatcct accccatatt ctctgaatta atttcatcct gaatgattca acctgaaaac
    18961 accatgatta ccgacatgct cggtcgaacc attaccctgt cgaaaactcc tacccggata
    19021 gtttcggttg ttccttctca aacagagttg ctgtatgatt tagggttagc tgaggaagtt
    19081 gtgggaatta ccaaatactg cattcatcct gatgattggt ttcattccaa aacacgcgtg
    19141 ggcggaacga aaaaaatcaa tcaggaaatc atcttaagcc tcaagcctga tatcgtgatt
    19201 gcaaacatgg aagaaaatac gcaatcggaa ctggaagaac tggctaaaca tgttcccgta
    19261 tggatttcgg atgtgcgtaa tttgaatgat gcgttacgga tgattgaact tttaggtgat
    19321 ttggtagaca aaagcgaaga ggcggaaaaa atcatcctca aaattcgaag cgactttgat
    19381 ttattaaagc cgatatcaaa acctcggaaa gccttgtacc tcatttggcg agaacctttt
    19441 atggctataa attccgatac atttatacat gatatgatga tgcgttgcgg attgattaat
    19501 gtgtcgtcgg aagcacaaac acgctatccc cagttaagtc cggaacaagt tcatgcgcta
    19561 aatcccgaat atgttttgct gtcatcggaa ccatttccat tttcagaaaa acacatagcc
    19621 gaaatacaac gactactacc gaaagcacga attggattgg ttgatgggga agcattttct
    19681 tggtatggta gtcgacttac ccaatcggcg ccctatttca aacaactcat tcgattttgg
    19741 gacaaataaa ttctgtattt ctttattagg tgcttatttt ccgtttctct gaaataatgt
    19801 aggtttgaag aataagaact cgcatttttc cgttttcgat agttgtgctg atacttcgta
    19861 tacttttttc cagttttctg cttctcgttt ctacttgtgt tttcggacaa aaagtgggcg
    19921 tagtgttgag tggtggcggg tcgagcggat tagcgcacgt gggtgtactt aaagcacttg
    19981 aagaagctca tattcctatt gattttattg ctggtacttc aatgggagct cttgttggtg
    20041 gaatgtatgc catgggatat tctcctgctc aaattgaact tttagttaag agcgaggagt
    20101 ttgaaggttg ggtgtttggt aaacccgtcg atcgctatgt ttattttttc agagaacgag
    20161 aacccgacgc taactgggta ggcatcaaat tccggattga ttcggtaatt caaacgcgct
    20221 ttccaaccaa tatcatttct ccagcgaata tggattttgc ttttcttgag cgaacgtccg
    20281 ccattagcgc cgcagcagga tataatttcg atagtttgtt tgttccctat cgttgcgtag
    20341 ctgccgacat ttataataag gctgaagtcg tatttaagga tggagatatt ggacaagcga
    20401 tgcgcgcttc atccacttat ccgtttttct ataaacccat tgtagttgat gataaactat
    20461 tgttcgacgg aggtttgtac aacaattttc cggctgatgt gatgtatcgt gatttcttgc
    20521 cggatgttat tattggaagc acggttgcat ctgaaattag acctcctcaa gaagatgata
    20581 ttctttctca aataaagaat atgttgatgg ataaaaccag ctatggcgag gtgtgtgaaa
    20641 ccgatcacat gatcatcata aaaccactca ttcctaaggt tgcgatttta gattttaatc
    20701 aaaccgatgc catgatagaa ggtggttatc aaagtacgct tgctcgttta ccccaagtga
    20761 aaacaatgat ttaccgcagg caagattcgc tcgaattggc taatcgaaga attgctttca
    20821 aggaaaaaca aaagcctgtt gtgattgacg atattaacat cgaaggcttg agtaaaaatc
    20881 aggttaacta tataaaacga gcttttggag agaaggcatt ccctttacca attgaaaata
    20941 ttcgtcctta tttctaccga gttgcatacg atgaacgcat taaaaaaata tatccaacag
    21001 cccgttatga ttcaaaagac ggacattttc tgttgaatct aaaaatgaag gttgacaaag
    21061 atttacaagc acaatttggg ggcttgattt cttctcgtcc gattaatact gggtttgtag
    21121 ccttgcattt aaaacgaatc aatcggattg gtttagcctt agatgccaat tcctactttg
    21181 gaaaatttta cggttcatta caggttaaag caacgctcga ctatccgttt aaacttccct
    21241 ttcaagttga aaccgatttt accttaaaca actttgatta tttctacagt tcgagtacat
    21301 ttttcgagga tgtaaaaccg tcctacctta ttcaatacga ccgttccata actggagcat
    21361 tagttttccc ttggaaaaat aaatctcgca tcaagattgg acttactggc gctcgtttgt
    21421 ttgatgatta ctaccaaact caatctttca cgactgccga cacagctgat cgaaccaact
    21481 ttaaccacgg ttcgttttgg atgaatttcg agcgtaatac gctaaaccga aaaatgtacg
    21541 ctaacagtgg ttcccagttt tttgtgaagt ttaggtattt ggccggacaa gaatttaaca
    21601 atccgggtag tacatcattg caaaacattg aataccgaag gatacacgat ttctggagaa
    21661 tgaaagctgt ttacgacgtg tatttcaaca aacgcggtcg gttacatatc ggtttttatg
    21721 gtgaggctgt cgtttcgaat cagccattgt tcaataatta tactgctagt ttattgcaat
    21781 cggcagcatt ttctcccatt ccggaaagta gaacattgtt ccaatctcag tttcgtgcac
    21841 ataattatgc agcggcagga ttaaaaacag tgctcagttt cacctctcga ctcgacttaa
    21901 ggctcgaagg atatatttat cagcctcatc gagttatatt agaaaaccca gacctcacag
    21961 cgagttatgg agatgctttt tcgaagcgat actttatggc atcggcggtt ttagtgtttc
    22021 attcaccaat tgcacccata gcggttagtg caaactttta tgatcaaagt aagaatccaa
    22081 tatccatttt gtttactact ggattcctca ttttcaatcg tagagctttc gattaattag
    22141 ttgggaccca atcctcccgt gtggatataa atgatacgtt ttttattccc gagcttctgt
    22201 tctaagaaga gagcatacaa tccaaacaat gcttttgctc cgtaaactag atctattgga
    22261 attcctgttt gttcaatcca ctgttgctga aatgctatta attcaggttt cgacttggca
    22321 tatccaccaa aatgaaagtc ggcgttaaac accaaattcg gatagtaact tgaatcaaat
    22381 ccgtgtttcc ttagttcttg ttgtaaaatc tgttctcctt tcaacacctg gaaacacaac
    22441 actttactga gacttttttc aagtttctga gctattccag ctgcacttgt tcctgttcct
    22501 ccaggaagta ctatccaatc gaatggctca tccgactgaa ccatttctgc agcacctaaa
    22561 attccaggca atccagctcc accttctggt acaattcgac acgaacccaa tttatcggtc
    22621 cagttttgca gaaatccatg atcggttttc tgtcgatagg attcacgact aacgaaatga
    22681 atttccattc cattctgctt catagcttgt aaccacgggt tttctacgat gtcgccccgt
    22741 acaattccaa tggtgcgaat tcccgtttct cttccagctg ctgataatgc caataaatga
    22801 tttgaatacg caccaccaaa ggaaacgaca gcttcgtagg cgggtaaatc tttcaaccaa
    22861 tgaatcaatt taaaccactt attaccacta atttcagggt gaagcttgtc gaatcgcctc
    22921 actgttaatt ccatttctgg cttacctggt aaagaaaagc aagtgacttg atcgtaatgt
    22981 gatagcggtg ctaatgaaat catcctgcta agcaaacaat ttctgccgtt cgagcattct
    23041 atgccttctt taaattttgc gaactttgaa gcccaatgga aaacgaagga ttctcaagac
    23101 tatctaatct cgataaagac gaatactatt attcgcccga aggatacgtc gttttcactg
    23161 ccaaatatct gctcaaacgc ggctactgct gcaaaaatgg atgcagacat tgtccctata
    23221 actacaacat ccgcacagga aaattcgata aagacgcaca tggcaactct tagcacaacc
    23281 gaattagaag catttcaaca cgatttactt gctggaattc ctaccgaact tcctcctact
    23341 cgtaaaccca atccagccat cagtcatgcg cctaggagaa aagacattct ttctgccgac
    23401 gagaaaaaac tagcattacg gaacgcattg cgttatttcg aaccacgtca tcatgccgtt
    23461 ttagctcccg aattcatgga ggaattgaaa caatacggtc gcatttacat gtatcgtttc
    23521 cttcccgatt ttcacatgca tgcgcgacca gtcaagcatt atccggcaaa atgcaaacaa
    23581 gctgctgcca ttatgctcat gattcaaaat aacttggatc acgccgtggc acaacatccc
    23641 gaagaactca ttacctacgg tggaaacgga gctgtttttc aaaactgggc acagtaccgc
    23701 ttaacgatgc aatacctctg tcagatggaa gaagatcaga ctttggtgat gtattcggga
    23761 catccgatgg ggctttttcc ttctcataaa gatgcgcctc gtgttgtggt aacaaatggg
    23821 atgatgattc ccaactacag caagcccgac gattgggaga aattcaatgc gttgggcgtt
    23881 acgcaatacg gacaaatgac cgctggatca tatatgtata ttggtccgca aggaattgtt
    23941 catggcacaa ctataactgt gatgaacgcg gcgcggatgt gtttacgtaa acgtggtgat
    24001 ttgcagtcgg atgtgattga aggaaaagga ctactattcg tgactgccgg tttgggtgga
    24061 atgagcggag cacaacctaa ggctgcaaaa attgctggat tggtgagtat tactgcggag
    24121 ataaatcctg cagctgcccg aaaacgacat gctcaaggtt gggttgatga gttacatacc
    24181 gatttggatg ttttgatttc tcgttgcgaa gaagcacgtg aaaaaggaga agctctttcg
    24241 ctagcttacg aaggaaacac gattgatttg tgggaagctt tggttcgacg cggaattcga
    24301 gtggatattg gttcggatca aacgtcattg cacaatcctt ttgcgggtgg atattatcct
    24361 gctggattta gtttagaaga gtcgaatcgt atgatggctg atgagccgaa acgctttcat
    24421 gatgaggtgt ataagagttt ggttcgtcat gcagcagctg tgaataagct ttcggatacg
    24481 ggcatgtact ttttcgatta cggaaatgcc tttttgttgg aatcttcccg tgctggtgct
    24541 gatgtgaaag gagcaacagc tgagttccgc tatccatcgt atgttcagga tatattagga
    24601 ccgatgtgtt tcgattatgg attcggtccg tttcgttggg tttgttgttc gggcgaggca
    24661 gaagatctcg atttgactga tcaacttgct gctgaagtat tggaagagtt gctacaaaat
    24721 gctcccgatg atattcgtgg acaaattcaa gataacctca actggattcg tgcagctaag
    24781 aaaaatcaat tggtagttgg ctctcaagca cgtatcctat atgccgattc tcaaggtcga
    24841 attcaaattg ctttacgttt taaccaagct attgctgatg gaacgattcg cggaccgatt
    24901 gttttaggtc gtgatcatca cgatgtttcg ggcaccgatt ctccttaccg cgaaacctct
    24961 aatatttatg atggaagtcg ctttactgct gacatggctg ttcagaattt tgccggtgat
    25021 gcctttcgtg gtgccacctg gatttcgttg cacaatggcg gcggtgttgg ttggggcgaa
    25081 gtaataaacg gtggatttgg gatggtgatc gatggcacgg aagacagtgc tcgtcgaatt
    25141 aaatcgatgc tccattggga tgtaaataac ggaattgctc gtcgcgcttg ggctcgaaac
    25201 aaagaagctg ttacagccat aaaacgagcg atggaactcg aacccaaact taaaattact
    25261 gttgcaagcg aagttgataa taatattatt gattcgcttg attaacaaat agatatcata
    25321 aattattata tatattaacc ttgcaatttg gaatttacgt ttccaatttg aaaggttatt
    25381 tatatatttg ttgcatgatt ataagaaggg caattcatga gattcacgaa ttgctggaag
    25441 attttccggt agttgcgatt attggtcctc gacaagttgg gaagtcaacc ttggcgaaag
    25501 cgcttgaggg cgatcgcgaa atcctctata ttgatttaga aaatcccgca gaccgagttc
    25561 gattaggcga tcctgttttg ttttttgaaa gcaatcgaga taagttggtc gttttggatg
    25621 aaattcagtt tgtgccagat ttgtttcctg tcattcgaag catggcagat aaatatccgg
    25681 agaacggacg gtttttggtg ttaggttctg catctcccga tttgttgaag caaagctccg
    25741 aaaccttggc tggacgaatt gcctatgttc gcttgtatcc cttttcgtat gctgaattgg
    25801 agaatgagcc ggattttact tggcaacaat tgttgcttcg gggcgggttt ccaaaaagct
    25861 atttagcacg taatgatagc gctagtttaa cttggagaaa tcagtttatt caaacgtttt
    25921 tgcagcgtga tttagccaca ttgggattta atttgaatcc agctgggatg tacaatttat
    25981 ggatgatgtg cgcgcattat catggtcgga ttcttaacca tagttccata ggaaattcgc
    26041 tgggcttaag caataacacc attcgaaacc atttggaaat tctcaaagga accttcatgg
    26101 ttgatgtctt gcaaccttgg cttccgaatc tttcgaaacg actggtaaaa tcgcccaagc
    26161 tttacatttc cgattgtggg atattaaatg ctcttctgtc ggtgcgaaat ttttcagatt
    26221 tactagggaa tccaattgcg ggttttgcct gggaaggact ggttttacaa acgatgcggg
    26281 cgcaattacc tgccgattgg aacatctatt atttatctac tgcagctcag gctgaaattg
    26341 attttatcgc cattcgtggc aatcaagtgc taggaataga atgtaaattt tcgacaagtc
    26401 cggctttgag tcgtggtgca aaagagttga ttaaggattt gaatctggaa gaggtgattg
    26461 ttgtaagtcc ggtggaaaat gatttcctaa tgagcacggg tgttgatgtg ctgtcgctga
    26521 agagtttttc ggctcgattg gtaaatgtaa agtatcagta aagcagaacg tcgcgccagc
    26581 gattgaagcc attaccggtt ttgaaccgaa ggtgaaaaag ctgtagaggc gtaaagcgcg
    26641 gttcaggcgc ccaaatcaac gaagttttaa ttgttgtcct tcgctcggtt gagctccttc
    26701 ttcgagttga tttaggtgat ataaacgact cagctttatg gcgtgttcct gtgaaatgga
    26761 ttgcatggtt tcgccagatt taacggtatg tgaatcggta aaagccttgc gacgcttggg
    26821 ttgtatgtaa attacatcgc ccgctgcaag tttagccgaa ggaggcaaat cgttgtattt
    26881 gggaagctgc caacgaagca tatccaattc ggtggtgagt gcttcgaacg tgtcgccttg
    26941 cttagctacc acatattgca cccgattacg caccatgatt ggccttccgc cttgtttttt
    27001 caattcaaaa tcgggcaaat cggtttcgtt agattttcct cttggtgttc gcggattgga
    27061 tatttgggtt ggttttttat ctcggtttcg aactggtttt gactccacga cagctccacc
    27121 tttctgatca aactcataca agcgattatc ctcaatcgtt ttgattaaca attgcgcata
    27181 ttgtggattg gtagcatatc ctgctttttt taaaccgtgt gcccagcctt tgtaatcggt
    27241 tatttcgagt gtgaatagtt cggcgtaacg cgtgctgttt ttaaggaatt gtgaatgatc
    27301 ctcatacgat gctagaaccg tttcgtattt acggaaacac tcatcttttg catcatcgtc
    27361 tttgtgaaag gatgggccag tccaatcttt tttgcattta attccaaagt gattgtttgc
    27421 ttcagtggcc aagtcactat tcccggcaga tgattctaaa agaccttgtc cgagtttgat
    27481 agatgcagga attccatacc gttccatttc ggttatggcg atatctttat aagctgcaat
    27541 atattcggaa gctgtttgtt tacgctgtgc agaggctaaa acgggcaaag cgagcagcgt
    27601 aaaaaggaaa aatctaatca tgtggcaaag aacgtatttg ttctgttttt tcgtttgggt
    27661 gcgacatgga gtttttaacg attgagcgtt tacagcgaag ctttatcaat tcggcacatg
    27721 ctatcccttt ttttctacct ttgcccgcta tttttacttt gccccttcta tgaagttatc
    27781 ccagtttaaa ttcaaactgc caccagaact gattgcagaa cgccctacgg ataatcgtga
    27841 tgagtcgcgt ttgatggttc taaatcgtaa aacaaaaact atcgaacatc gcatgttccg
    27901 tgatattctg gaatattttg acgacggcga taccttcatc atcaataaca cccgtgtttt
    27961 tcctgctcgt atgtacggga acaaagaaaa aaccggagct aaaatcgagg ttttccttct
    28021 tcgtgaactc aatgccgaaa gtcgcctttg ggatgtgttg gtagatccag cacgtaaaat
    28081 ccgtattggt aacaaacttt attttggcga tgatgattca ttagttgctg aagtaattga
    28141 taacacgact tctcgtggtc gtaccttacg tttcttgttt gacggtccgc acgaagaatt
    28201 caaaaaagta ttgtattcat taggagaaac tcctattcca aaatacatca atcgcaagcc
    28261 ggatgaagaa gacgaattgc gttttcaaac cgtatatgcg aaaaacgaag gtgctgtagc
    28321 ggctccaact gctggacttc actttagccg tgagctaatg aagcgcatgg agttaaaagg
    28381 cgtgaacttc gcagaaatca ccttacatgt tggtttggga agcttccgca cagttgaag
//
LOCUS       DAOOQW010000009        27640 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_2030_length_27640_cov_5.383107, whole genome shotgun sequence.
ACCESSION   DAOOQW010000009 DAOOQW010000000
VERSION     DAOOQW010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 27640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27640
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2030_length_27640_cov_5.383107"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2962)
                     /locus_tag="PK281_01350"
     CDS             complement(<1..2962)
                     /locus_tag="PK281_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48013.1"
                     /translation="MFRFLTSLFICLFILNCSSNAQVSPCNQVVADPQGAGVSYLPNL
                     VFLDTLSGDANQTLTLDFSLIDLAEGDTLRIYDGVFLNELAVFTAGSPLDSIQSLSSS
                     LILNFSSDQAFEAEGWEATVNCQPANFIQLTFPTDTICAGWEGTTDFTLLANFPLDLY
                     IEAILSDANGDFSSPIPIGETSNSDTTLFLAFAANLAASESYKIRIQINLSDSVLIYQ
                     DIPLHINTVPLQPSISGLSTFCGDSITLSTEDQFRIDFQWFLNSDSIPSATNINLNTN
                     QAGTYVITASNSCGTLSSSSFILTQIQLPATAVLQTTDSQLCPGEEASISYTADGDTS
                     DFTWIRSGLQLSFTSEEIQTTIPGEYVLSRSNQCGSSLSDTLLIEAIPLPPAVLIQAA
                     GPLSFCESGVVQLNIPNADNNQIQWFRDSILIQTGAESFEASLSGTYSVSLSNLCGTT
                     PSINTTEISVQLNPVPALLSAAGPTTLCEGNSVLLLAEVANGQQVSWLFNGQPTGNSS
                     LQLSADQTGVYSLTTSNSCGSVTSQNNIPVTINLLPFVPTLYSLSTPALCNGSSVTIA
                     VEAQTGVTYAWKRNGSAYTGTTNSITTSQQGVYTVSVSNGCGTLLSTNTISVISGSAP
                     VNPTITAGGTTTFCQGLSVTLTTPPQQGVIYRWLLNGIPTGDESFSMQATQGGAYTVE
                     VSNACDTLVSTASLPVNVLPLPIQVEVNPIGEYSICQGDSLELSIPVVPGVSYQWRRN
                     NQPVGLNTNSIDAFQEGSYTIVLGNSCGTTPASNSVYLNIDSIQPALSAIVAQPGTAL
                     CPGGYVLLNAAPVPFQLYHWYLDGIQIPNQTSAVLQATEWGTYTLQTNNACGTSAESP
                     SVTLGPGDPPSDFEIYITGDPIFCSNDSLAITAQAEFGVSIRWFLNDVFLVEGPAQIY
                     VHQAGVYTANCWNGCGEASGTNQLDISTIPAPEIPIITLSGNSLTTNASGTLQWLDAT
                     YTAIPGETSALFTPE"
     gene            3059..4333
                     /gene="serS"
                     /locus_tag="PK281_01355"
     CDS             3059..4333
                     /gene="serS"
                     /locus_tag="PK281_01355"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006260406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOY48014.1"
                     /translation="MLQVPYLRENKEAVIAGLAKRNNLDARERVDQIIALDDMRKSVQ
                     TELDSKLARANQIAREIGELFKSGKAAEANVLKEETATIKEESKNLEGKLAEAEEAIR
                     QILYTIPNVPATAIPFGRGSEENVTVESHGEIPVLHADAQPHWDLAKKYDIIDFELGV
                     KVTGAGFPFYKGKGARLQRALINFFLERATAAGYTEYQAPLMVNADSGFGTGQLPDKE
                     GQMYYMQVDDFYMIPTAEVPITNIFRDTIQKAEDFPIKACGYTPCFRREAGSYGKDVR
                     GLNRLHQFDKVEIVQIAHPDQSYTLLEEMRNHVTGLLKELELPYRVLQLCGGDMGFAS
                     AFTYDMEVYSAAQGKWLEVSSTSNFEAFQSNRLKLRFREGDSKPRLAHTLNGSALALP
                     RIVAALLENHQTPEGIRIPKALVPYCGFDLLN"
     gene            complement(4407..5297)
                     /locus_tag="PK281_01360"
     CDS             complement(4407..5297)
                     /locus_tag="PK281_01360"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOY48015.1"
                     /translation="MKLNTIKPIDSEVILVPYDFTEAAQNAVAHANLLARLFHKKIVL
                     LHVVEKGLFKAGTPNTLSEKETDKKLKGIAATNTADQKIETSHQLRSGNIFDTIADAA
                     GDLNAALVVMGTHGVTGMQHVTGSKALKVITNSNRPFVVVQKKDVRLHGYKNIVLPFD
                     FSKETKQKLVWAVELSKVFNSTFHILAAYESDEYTAKAVSNNIAYAKNYLKSRDCDFK
                     VTTAEKGDFSKETIRFASYVEADLIIIMTNQGKSLTDFIIGPYEQNVIANDAQIPVMT
                     LNPVDNMNSVNGHLFNFGNF"
     gene            complement(5449..6180)
                     /locus_tag="PK281_01365"
     CDS             complement(5449..6180)
                     /locus_tag="PK281_01365"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOY48016.1"
                     /translation="MKHELFSWYSPALNAEMPLAIYGHYGFSILLIPTAAADFLEYQR
                     FQMMDSLAPFLESGKCKIYSINSINKESWLNNDMEPAHKAIRHNQFNDYVINEVIPFI
                     KQTSSEETPIYTCGASFGALHAMNLFMKYPDKINGAISMSGVYNLAEYTKGFWDDQVY
                     FNSPEHYVQNINDPVYLDQICSSKHIHIYTGSGDYEDPNANIRFSNVLASKGIPHDLD
                     VWGPDITHDWPTWRKMLPYILDTKF"
     gene            complement(6312..7229)
                     /locus_tag="PK281_01370"
     CDS             complement(6312..7229)
                     /locus_tag="PK281_01370"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HOY48017.1"
                     /translation="MKTVLLTGATGLIGSHILPILKQQGFRIHLFSRSANQVAHADAV
                     FLWNLESGELDEQAFDGVSHIIHLAGAGIAEKHWTDERKAEILSSRVKPAAMLLKALL
                     KRNQQLEAYVSASAVGWYGAITDNHLHAEVESAAEDFLGETCRLWEQSADAFESISNR
                     VVKIRTGVVLARESGALPQMMKPFKYYSGSPLGSGKQQIPWIHIEDIARVFAEACMNT
                     NWTGAYNASATTSCSNRDFSKALAKTMNRPLLPISVPSFVLKLILGEMSVVVLEGSRI
                     SNQKIKNAGFEFNYTKLESALENLISIQN"
     gene            7358..8365
                     /locus_tag="PK281_01375"
     CDS             7358..8365
                     /locus_tag="PK281_01375"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HOY48018.1"
                     /translation="MNQLLFIETFVLGSAVIAAIYHLVLFIQQRDKFLLFYSLYLFSL
                     SVYIGFKLISNNYDPFLPTDNVWYYILEEVLQVAMVTVYVLFAAQTLEVVQEENIVRS
                     FMYAFFALSAFSICYHVFDAIQNGPGVKSTFEYAFSRISLVGIATIALISAWRIRTTT
                     FQRTIIIGSLVYDSSGLLSIISFTQKTDILGLNGVEPYLAGCLLDIIIFSSALGYRLK
                     TIAVQKNELLIKESQARLAIEHTRTQIAVNLHDDVGSILSSMSIYSEAAKQSVRENNL
                     PRVTELIDKIGISARETMGNMSDIVWTINPINDNGIKLFNKMEDFASPLFHSKQVNFS
                     F"
     gene            8399..8674
                     /locus_tag="PK281_01380"
     CDS             8399..8674
                     /locus_tag="PK281_01380"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOY48019.1"
                     /translation="MPVRQHVYFIFKEAINNCIKHAEAQHVKVNIDKIQNDYVMRIED
                     DGKGFDNEKDYNGNGYKSMKQRASELNGTILFKSANPGTILVLMFKL"
     gene            8743..9405
                     /locus_tag="PK281_01385"
     CDS             8743..9405
                     /locus_tag="PK281_01385"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOY48020.1"
                     /translation="MNIPMAVRVTIFDDNQRLLDSLDALIGGSPGFQIAGIFSDCSDI
                     QSKIEKSQPDVILMDIEMPGINGIEAVKLVKRYFPRINVLMQTAFENDELVFEAICAG
                     AQGYILKNTPPARILECIVEVYQGGSPMSPSVARKVLGFLQRPDKKTEETISPNNYNL
                     SPREKEVLGSLVNGMSYKMIADQHGISYETVRSHMKNIYEKLHVASMTEAVAKAINQK
                     LV"
     gene            9495..11186
                     /locus_tag="PK281_01390"
     CDS             9495..11186
                     /locus_tag="PK281_01390"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY48021.1"
                     /translation="MKNLFALFLILFYHVSFSQTLITDTLFGESGYVSALNVGNSEVM
                     TILQQPDNKIIVGGHNYDFGCNCHYIDMIRTDACGMIDSTFGINGIVHYTFEQRNLGF
                     NYKLQPDGKILVVGVQSDGNNGSQQYPFIARFNSNGTPDTTFGDHGSRKINSIGACEF
                     LNVYLLDEGKLLCTGGPTNYSNVIMQFDSTGNVDPTFGNNGVVQHSIPAGINFFNSFF
                     SMMRTDGKIISVAPAWMGGDFRQAVLSCFDTQGELDTTFGNQGFFIDYDFYIANGFKY
                     AIQSDDKVIIAQQNTTETAMNIRRYNTNGSVDTTYGNGGTVSIPAPNSPSRIQFLTLL
                     ANDDVIIGYQENGDVPKYKKVDVNGLEVTDFTLNGGSSFQFPTNDDKGRVALTTSSGE
                     MVIAGSQNSFSLTRFIANTSIPNITISGVTLNSNVGNLESTFQWFLNGNLIANETSSS
                     IEISQNGMYTVEVTNAWGCNTSDQLEVINLGEESISSIKFSVFPNPAEQILTIQSKDN
                     TLISIRIFDVSGKLIKSETSNATFTNIKVDEFTSGLYFLEIKSENQITQTKFIKK"
     gene            11333..13036
                     /locus_tag="PK281_01395"
     CDS             11333..13036
                     /locus_tag="PK281_01395"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY48022.1"
                     /translation="MKKIILYLFLINAPQVFSQALVQDQLFGTNAESFSLPTGTTSET
                     NVLFQYPDKKVVACGYTYDIGCNCFYNIMFRVDACGQIDSSFGVNGLVRHTFDQRNAG
                     YGYTLLPNGKMIVVGMQSDGNSGSQQFPFIARYFYNGQPDTTFGDHGTRKISTIGPAD
                     LTSVYQLDTGKLLCTNGPLMMRFDSLGVLDPTFGNNGILIQSVPSPFNFYYQSNSVMR
                     SDGKIISVSSVYTGVDNDKYVTQMCYDAEGLIDSSFGVNGFSIDYNFVVGSAPPRLIL
                     QSDDKVIAIRQNITETAIIIARYNTNGSLDTSFGSDGYLSLPSNRLVYVSNFNDDSFL
                     VGVSQGGVPIQYFRVSANGIVDPSFTLNGSVYFQTMGNNGPSPQAGLAFGNNEIVMAG
                     CNSGGNGSTAIAFTKFALTSGIATITQIDNTLNANLPEDQFTFQWYRDGVLVAGDTTA
                     AITVFQNGEYRVVASNLAGCSSTDTITVLSTGGQTLRQASDVKIYPNPSTGYFQLKFN
                     SEIKSGIISVIDISGKLILEKQFNHSSTLNVDLTSLSKGIYFLKINTDNGIQIHKIII
                     Q"
     gene            13046..14710
                     /locus_tag="PK281_01400"
     CDS             13046..14710
                     /locus_tag="PK281_01400"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY48023.1"
                     /translation="MRIFLFFIAICLTTGLNAQFTSDSLFGINGTVSATQVGAKATAI
                     LRQTDDKIIVCGNLDYNFNDANQMFRVTACGEIDSSFGVNGSVQHTFEFQNLGFDYVL
                     QPDGKILCCGIQSSDNAGSQQFPFIARYNSDGSVDTTFGTHGSTKISNYGSNSFGNIF
                     LMPDGKIICSSGPFAMRFLADGSVDFSFGNNGILSMPTAPNGYYESRGLSNVLRSDGI
                     IVGANTVLDFNFSYPSLGVVAYDTSGVVDSTFGNNGYLQDFAGGIAAYSVRTILQSDN
                     KVIIAGNNPNGIHITRVTTSGQIDTTFGISGYLDFSPGGELFALIKLNNNHFFVGYRL
                     AGTGNFFRVFDSNGVLDTAISLNGSNNNMGDLGILNVALVESNGDITFGGTSLGFPYR
                     ISRLVKSSAIPTITLNGDTLFSNVADNSCTYQWYMNGNLIEGANNSNYSITANGNYSV
                     QVANIWGCSISDEFNVTTVGIDRVEDYSVSVFPIPATNTLTIQYSKPLMQASLFITDL
                     AGKIVLEKRENNTSTTLLNIAGFAKGIYILQITSDQGNSSHKIILE"
     gene            14829..16910
                     /locus_tag="PK281_01405"
     CDS             14829..16910
                     /locus_tag="PK281_01405"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HOY48024.1"
                     /translation="MKKIKYTFLLLAIFSLSISVTSQTFQWAAQYGATLNSDGKAIAT
                     DSDGNVYTTGSFLGTADLNPGSGTFNLTSAGRDIFISKLDPTGNFIWAKKLGGSSIDD
                     VYAIALDDAGNVYTTGTFSGTADFDPGTGTFNLTGGAQNYGVFISKLDPSGNFVWAKK
                     IELTDGGNSFENTPSSIKVDAIGNVLIIGNFNIPADFDPDEGVFLLDPDITTSAISDI
                     FILKLDASGNFVWAKQMGGTSFDYGNSISTDADGNVYTTGYFNATADFDPGVATFNFT
                     SVSNDIFISKLDAAGNFVWAKQISGPGADRGTELTIDESGNCYVAGFFSGVTDFDPGI
                     EVVNLTSAGLYDVFILKLDSSGDFLWAKNIGGAGYDEVYALAIDDSNNVYTTGIFNYS
                     ADFNPGSGVFNLTSADVEDIFISKLDSSGNFIGAKRIGGPDSQKANSLVLDNTGKICL
                     TGYFLGGGTDFDPDEGEFIFTSNVNRTAFTLKLNDFSSSCSSSSNSIFQSSCGTYLFN
                     NQLLTESGTYTDTLVNGAGCDSILTLNLTINAIDNTVVQDGNTLTANGSGTYQWVDCN
                     SNFSAISGETNPSFTISSSGSFAVIVNNEGCIDTSACFLALGIIDFNHLQSVSVYPMP
                     VSNMLTVEFSKKLTNASIVLIDLAGEKVFEGKQSNTSVIVLDISGLASGIYVLHVNSD
                     QGNSTKKIVIQ"
     gene            complement(17464..19050)
                     /locus_tag="PK281_01410"
     CDS             complement(17464..19050)
                     /locus_tag="PK281_01410"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY48025.1"
                     /translation="MAIVLTSNFCFSQSGYLDHTFNNTGFNLSISADYPMLIAYDMLV
                     QPDQKSVVVCEGFTSSGRDLVVVRYNVDGSLDESFGTGGFVSYDQQGMVERVTSVALQ
                     SDGKLIAGGNMYISEGDINPTKWFAVRFLQDGTLDTSYGVSGAALIGSAEFVPLKTYD
                     SAVQSDDKLLLVGETNTTGYDRGTTVRLDINGQLDLTFGINGVFYPYPQEVYNGIMGV
                     KIQPDGMIVVCGDLLVQPSLSENDYLIMRFDQNGVMDNSFGNGGVVTLDAGPLYGSLM
                     YISFQGDGKIVCSGLYYAGLATGGYCTSVIRISEQGEIDPSFGANGLVKIEYNPIHEE
                     LPYGVEIKSDGEIVVAFATPQLPPAQNSFFMIKKLNSDGTPDQSFGVDGLIQLNMTFG
                     DAIHAMAIQSDDKIVISGIGTNQNDNMQTVLIARILPDELISSISEKAFVVNSSVYPN
                     PAQDILQLSFELPNAEIGSIKMFDVSGKLIRTYIEKQLLFQGINNFTLDISHLTSPGV
                     YILELNTADGRKSTVQFVKE"
     gene            complement(19171..20775)
                     /locus_tag="PK281_01415"
     CDS             complement(19171..20775)
                     /locus_tag="PK281_01415"
                     /inference="COORDINATES: protein motif:HMM:TIGR02608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48026.1"
                     /translation="MNSFLALLIISSRAFGQNELVDLSFNQSGLVITPEGLGNEAQFY
                     SCVIQNDGKIIAAGGTNITISSPSFQGNELLLIRYHSDGVVDSTFGIDGVVRVNLAEA
                     ATFARTIRLRPDGKIAVSGTYRLYENNPFYTNYAFMAVYNDDGTPDLTYGINGMSVII
                     PFEGQAFIPEEMVIQPDGKSIQVGISYYLGQTDLVMARYNTDGTPDVTFGNNGLVIQD
                     YNQQLQTGRCLTLQEDGKILAAGTLIISSDESPATIFRFLPDGQLDPTFADAGIFILN
                     QAPLLAEFFGVEVQPDGKILAAGNNNTNSSNSVFMCIRLDANGNLDNTFSSDGIFRMD
                     ELGGLHNDLLWDMKLNSNGKILLAGQGTATPFNDFPSKATLVQLKEDGTLDPTFGTNG
                     LHFTDLTSNDEVCSHVEFQDDGKILLAGIAADTGGYKGSLVRLLPSSTVNMDKLDVQT
                     SLALFPNPLDERAYVNISLKSKGLRSLFLVDFKGQLVETIFLDKAMNCGEHTVPFEVN
                     SKIVPGMYKLVLVSEDQTHSTSIIIK"
     gene            complement(21217..23316)
                     /locus_tag="PK281_01420"
     CDS             complement(21217..23316)
                     /locus_tag="PK281_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48027.1"
                     /translation="MKATRINIQILSLLLLGICSSTFAQKDSTFIRIDTCRIKIISQV
                     KSDSIVLRWAPDHAYTWEMQLANGFIVERVEIPDDTSKPVVKVLLTNAPLKPLAKEEW
                     LKNFGKDNAMAMIAAAQMYPELTPYANPDPILIQQNQNKLQTRHGFALFAADNSPKVA
                     DASGLRYVDRSFEKGKNYLYRVFYSKSIEGLFSDTALVYINSSELSKIQIARAPAIIG
                     GDRKVELRWDASQNSGFSAFHIERAPSGTKQFIRLNKNPFVSFKPEFTKENNAYFTDS
                     VENYILYDYRIIGVTAFGELSSPSAVTTVMGRDLTPPSQAVITGVNSENKTTIRIKWK
                     IQQIDPDLKSLFVARGYDTDGPFKTISGELAKDVDSFLDSIPNPNGGNFYVVISKDTA
                     GNEIKSLPFYGIVEDNYAPETPIGLAGYIDSSSVVHLTWNLGKEPDLQGYRVYVANAS
                     DHEFSNITPAIWGDTIFLDTITKRTLTKYIYYKIVAVDNNYNHSAYSDWIKVRRLDVI
                     PPVAPVFGKVSVTDTTVHLNWVNSSSDDVKEQILYRKPAKDSVWLELKRWKFLDAETS
                     FTDRHLPKKQFFEYLLIAVDSSDLQSDSSSLVSIRTYDSGLRERIAELTVEFNKEKKS
                     TDLTWKYKNSGDFSFLIYRAMPGKPLTKYKMLKGTDRSFEDKTVSLSGEYSYAVKVIY
                     TDGGESPMSEKKVVQVP"
     gene            complement(23316..>27640)
                     /locus_tag="PK281_01425"
     CDS             complement(23316..>27640)
                     /locus_tag="PK281_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48028.1"
                     /translation="FRLNNTRVTAGVVADSGTYVSWDCQGFRILHLSGKLTFDESVLK
                     PATANGSIALKPVTASFGFNVRRSGNWLAALDFSPFMIPGVDDWTFKVDQATLDFSDL
                     ENPENIVYPDNYVGERSVLWNGFHLKSLRVGLPKKFKIRFPDTDVALADSVLKDRVVV
                     AINDLIIDRTGISGKLEALRVLEMENGLLGDWSFAIDTMSLRFVSNSFVSGGFNGRIK
                     TPLSPTLLKYESVLSQGAIATGIVYQFNVRSLDTLTVPIWAAQLQLLPTSRIIVNVDA
                     TNFRPELNLNGSVSITTTIGKVPVNFPGLQFQALRLTSAEPYLTCSAFTFASPQKGMA
                     GFPVTIGEIELGVQQDHGFFSWDDTPGSRIALKFTVEVNFTGEANSFSGSTTMAILGR
                     LNPGNLLTAADGEPVVYPSLNLTGIDLKSIYIDGDLGFVSAKGFVSFYAEDPLYGKGF
                     IGGIDATFIKTVNVSVIGSFGEINNMRYWYVDAMAKFVPGIPMGIAPYQIPMDIYGFG
                     GGAFYKMKFMSIPPPASTLTNASASELPVPGTTLSNVRLVPDASSRFGLKATVVLGTT
                     GGGTAFNADLTLGATFSNSGGLSQVAFSGSGYFMTSVMDRSFTNVRADVVLQYDFPNK
                     ILTGNFGVMVDVPMTLKGTQAKNMAGNAFLYISKPKWQLKLGEPTPQTAQVGLLIGGV
                     FNAKGYLMTGMDLPAPAPVPSKVTDLLGMVPSQRTSKIGTGQGYAFGASFIPPKINQD
                     YSPFYCNLDAEIGFDMTHFDHGSERCDGMLANEKMGVNGWYSTGTGWGYMRGSLGVVA
                     SLFEDNTHYPILDVNAAVLLQQGTPNPKWFKGTLGGNYSILGGLIEGVCRFEVQQGDF
                     CEPPPESPIGGFVLISDVSPNPSQTNVDCGVKPTVVFNIEIDKLISFQETGANGEKRT
                     RRFKFVITEFTLFDRTRNRTVLDVYDDYLLSPDGSQAMHNTSNLLSPFNQYRIIIAVQ
                     AREYLPNGEVIPATRNDGSLITERLVYSFETGELPNVLREEYIKFSYPLNRQRYFIDK
                     GGLGIRLNTNVPNLFDKNARQGYERSFYAQFQPVAGGQIIETPVSYTNGTFEVDNRTP
                     RSLGFISIGSPALLAPKTIYRLRIIHRDIRVSGTTATSGNLLVQNAGFQTVSLNKISF
                     NNGAVLLRDRQLEGAPVLRSNEHLIYEYFFRTSEFGTISAKINSLQVDNVEREYVDPR
                     ENLILNFKGPEGFERFEIDGFRYLDGFTTNIVRSMDIIDSYSNSWHSSFLMDKIYNTY
                     SAICDAQVYNHLSIYRPTPDDIGLPPNRISGTIRMKGPITNDEIYPSSNSSSSTSYIL
                     ANDPYAFADIHTADYVPQQQLILTTSYYAQMDYERMKVIIADMKARFGNNLRGYDPNE
                     RETYSNDYHRTGVPLAVRTKVTSFLNTPYQFMYYGPYTISMSGTFKYFNYPQVVTLLF
                     LRTN"
BASE COUNT         8589 a         5174 c         5371 g         8506 t
ORIGIN      
        1 gttcgggagt aaatagtgca gaagtttctc caggaatggc tgtgtacgta gcatcgagcc
       61 attgtaatgt accggaagca ttagttgtta ggctatttcc gcttagggtg atgatgggaa
      121 tttcgggagc tggaatcgtt gatatgtcca actgattggt tccagaagct tcgccacatc
      181 cattccaaca atttgctgtg taaactcctg cttgatgcac ataaatctga gccggacctt
      241 ctaccaagaa cacatcattc aaaaaccaac gaatggatac tccaaactca gcttgtgcag
      301 tgatggcaag actatcgttc gagcaaaata taggatcacc agttatataa atttcgaaat
      361 cggatggagg atcgcccgga ccgagtgtta ccgaaggaga ttctgccgat gtgccacagg
      421 cattgttagt ttgtagcgtg taggttcccc attcggtggc ttgtaaaaca gcagatgtct
      481 gattagggat ttgaattcca tctaagtacc aatgatacaa ctgaaaagga acaggagcag
      541 cattgagtaa cacataacca cccggacaaa gagccgttcc cggttgagct acaatggcag
      601 aaagagctgg ctgaatagaa tcgatattga ggtaaaccga attggaagct ggagttgtgc
      661 cgcatgaatt gcctagaacg atggtataac ttccttcctg aaaagcatct atgctattgg
      721 tgtttaatcc aaccggttga ttattccggc gccactgata actcacaccc ggaacaacag
      781 gaatgctgag ttctaaacta tcaccctgac aaatgctgta ttcgccaata gggttaactt
      841 cgacttgaat aggaagaggt agaacgttga cgggtaagct tgccgtggaa accaaggtgt
      901 cgcatgcgtt agaaacttca acagtataag caccaccttg agttgcctgc atggagaaag
      961 attcatcgcc agttggaatt ccattgagta gccaacgata aataactcct tgttgtggcg
     1021 gtgtagtaag cgttaccgac aatccttgac aaaacgttgt agtccctccc gcggtaatag
     1081 taggattcac aggtgctgaa ccagaaatta cgctgatcgt gtttgtgctt agtaatgtgc
     1141 cacatccatt actcaccgaa acggtgtaaa ctccttgttg tgaagtggtg atgctattag
     1201 ttgttccggt ataagccgaa ccattccgtt tccaagcata ggtaacaccc gtttgtgctt
     1261 cgacagcaat ggtaactgaa cttccattac aaagtgcagg agtactcaag ctataaagtg
     1321 ttggaacaaa tggcagcaga ttgatggtaa ctggtatgtt attttgggaa gtaaccgatc
     1381 cacaggaatt gctcgttgtt aaactataaa cacccgtttg atcggcactc aattgcagac
     1441 ttgaattgcc agttggttga ccattgaata gccaagaaac ttgttgtcca ttagctactt
     1501 cagctaaaag caaaacgctg tttccttcac ataaggtagt tggacctgca gcactcaaca
     1561 aagcgggaac tggattgagc tgtacactta tctcagtggt attgatggaa ggcgttgtac
     1621 cgcagagatt actcaaggaa accgaatagg ttcccgacaa actagcttcg aaagattctg
     1681 cgcccgtttg aattaagatc gaatcccgaa accattgaat ttggttattg tcggcatttg
     1741 gtatgttcaa ctgcacaact ccactttcac aaaaactgag cggaccggca gcttggatga
     1801 gcacagctgg aggaagtgga atggcttcaa tcaagagcgt gtcggataag ctgctgccac
     1861 attgattgct gcgagataac acatattcac ctggaatggt agtttgaatt tcttccgaag
     1921 taaaactcaa ttgcaaaccg gatcgaatcc aagtaaaatc cgaagtatcg ccatcggctg
     1981 tatagctaat ggaggcctct tctcccggac aaagttgact atctgtcgtt tgcaaaacag
     2041 ccgttgcagg taattggatt tgagttaaaa tgaaagagga agaggaaaga gttccacaac
     2101 tatttgaagc tgtaatcacg taagtccccg cttgatttgt gtttaggttg atgttggtag
     2161 cagatggaat ggaatcggaa ttaagaaacc actgaaaatc aattcgaaat tgatcttcgg
     2221 tgctgagtgt tattgagtca ccgcaaaaag ttgataagcc tgatatactt ggctgaagtg
     2281 ggacagtatt gatgtgtagt ggaatatctt gataaattaa tacactatcg gaaaggttta
     2341 tttgaatccg aattttgtag ctctcgctag ctgctaaatt ggctgcaaaa gccaagaata
     2401 gcgttgtatc ggaattagat gtttctccta tgggtattgg acttgaaaag tcgccattag
     2461 catcactaag aatcgcttcg atgtataaat ctagaggaaa gtttgcaaga agggtgaaat
     2521 ctgttgtccc ttcccatccg gcacaaatcg tatcggtagg aaacgtaagt tgaataaaat
     2581 tagctggttg acaatttaca gttgcttccc atccttctgc ctcaaaagct tgatcactgc
     2641 taaagtttag aatcaaggat gagctgagag attgaatact atcgagcgga cttccggcag
     2701 taaaaacggc gagttcattc aaaaaaactc catcataaat acgcaaggtg tcgccctcgg
     2761 ctaaatcgat aagtgaaaag tcaagtgtaa gtgtttggtt ggcatccccc gaaagtgtgt
     2821 ctaaaaaaac aagattgggt aaataagaaa cacctgctcc ttgtggatcg gcaacaactt
     2881 gattacaagg agaaacctga gcattactag aacaattcag gataaaaagg cagataaaaa
     2941 gactagtcag aaagcgaaac atagcagaaa gaagacgacg aaactacaaa gaactacaaa
     3001 aggagtgtgt taatttgtgg caaggcttaa ctttgccgcc gcttaaatag aaaatactat
     3061 gcttcaggtt ccttatctcc gtgaaaataa agaggccgtc attgctggat tggctaaacg
     3121 aaataatctg gatgcacgcg aacgtgtgga tcagattatc gcattggatg atatgcgcaa
     3181 gtcggtacaa accgagttgg acagcaaatt agcacgtgcg aatcagattg cacgtgagat
     3241 tggtgaatta ttcaaatcgg gaaaagctgc cgaagccaat gtattgaaag aagaaacagc
     3301 aacgattaag gaagaaagca agaatttgga agggaaatta gcagaagctg aagaagctat
     3361 ccgccagatt ttatacacca tcccgaacgt acctgctaca gctattccat ttggtcgcgg
     3421 ttcggaagaa aacgttaccg ttgaatcgca tggagaaata cctgtactgc acgctgatgc
     3481 acaaccacac tgggatttag ccaagaaata cgacattatc gatttcgaat taggggttaa
     3541 agtaacagga gctggctttc cgttttacaa aggaaaaggt gctcgtttac aacgtgcgtt
     3601 gattaatttc ttccttgaac gagctacagc agctggttat accgaatacc aagcaccact
     3661 catggtaaat gctgattccg gttttggaac gggtcaattg ccagataaag agggacagat
     3721 gtattatatg caagtggatg atttttatat gattccaaca gccgaggttc ctattaccaa
     3781 tattttccgc gacaccattc agaaagccga agattttccg attaaagcat gtggatatac
     3841 gccatgtttt agaagagaag caggttcata cgggaaagat gttcgtgggt tgaatcgttt
     3901 gcatcaattc gataaagtag aaatcgttca aattgcccat ccagaccaat cgtatacttt
     3961 gctagaagag atgcgaaatc acgttaccgg cttgttgaaa gagttggaac ttccttaccg
     4021 agtattacaa ttatgtggtg gtgatatggg atttgcttct gcgttcacct acgacatgga
     4081 agtatattct gcagctcaag gcaaatggtt agaagtgagc tcaacatcca atttcgaggc
     4141 attccaaagt aatcgtttga aattacgttt ccgtgaagga gattcaaaac cacgtttggc
     4201 gcatacactt aatggatcgg cactggcttt accacgtatt gtggctgcct tgctcgaaaa
     4261 tcatcaaaca ccagaaggaa ttcgaatccc gaaagcatta gttccatact gcggattcga
     4321 tttattgaac tgatcagata gaaaacaaga aaaccgtctt ttcctagtag tttaggagaa
     4381 gacggtttcg tttttttagg ggatgattag aaattcccga agttaaacag gtgaccgtta
     4441 actgagttca tattatccac tggattcaag gtcatcacag gaatttgagc atcatttgcg
     4501 attacatttt gctcatatgg cccaatgatg aaatcggtta atgatttgcc ttggttggtc
     4561 ataatgatga tcaggtcggc ttccacatat gaagcaaagc ggatggtttc ttttgagaaa
     4621 tcaccttttt cggccgtagt aactttgaaa tcacaatcgc gacttttaag gtaattcttc
     4681 gcgtaggcga tgttgttaga taccgctttt gctgtgtatt cgtcgctctc gtaagctgct
     4741 aaaatgtgaa atgtagaatt aaacacttta gaaagttcta cagcccaaac gagtttttgc
     4801 ttggtttctt tggagaaatc gaatggcaga acaatgtttt tatatccatg aaggcgtaca
     4861 tcctttttct gaaccaccac aaatgggcga tttgaattgg tgattacttt caaggctttg
     4921 cttcctgtaa catgttgcat acctgtaaca ccgtgtgttc ccatcacgac aagcgcagca
     4981 tttaaatctc ccgcagcatc tgcaatcgtg tcgaaaatat ttccggaacg aagttgatga
     5041 ctcgtttcga ttttctggtc tgctgtattg gtagcagcta ttcccttaag tttcttatcg
     5101 gtttcctttt cgctgagcgt attaggcgtt cctgctttga aaagtccttt ttcgactacg
     5161 tgaagcaaga cgattttttt atgaaatagc cgagcgagca aattggcgtg agcgacagca
     5221 ttctgggcag cttcggtaaa atcatacggt actaaaatga cttccgaatc gatgggttta
     5281 atggtattga gcttcaaggt atcgcgtttt aaaaaaatcg gttacgaaat taagatagta
     5341 atttaatatt ctggtacagg ccaataatta ttcgcatatg catgtaatac agcaagatgc
     5401 gacaatttag atgattcaat ttagctaatt gaaacggaga aaaaagcact aaaattttgt
     5461 gtccagaata tagggtaaca ttttccgcca ggttggccaa tcgtgagtta tatctgggcc
     5521 ccaaacgtca aggtcgtgtg gaattccttt gctcgctagc acatttgaga aacgaatatt
     5581 ggcattagga tcttcataat cgccacttcc ggtatagatg tgaatatgtt tgctggaaca
     5641 gatttgatct agataaacag gatcgtttat gttttgaaca tagtgttccg gactgttaaa
     5701 gtaaacttgg tcatcccaaa atcctttggt gtattcggct aagttataca cgccactcat
     5761 gcttattgcc ccgttaattt tgtcgggata tttcataaac aagttcatcg catgaagcgc
     5821 tccgaaacta gcaccacagg tgtaaatagg agtttcttcg ctactagttt gcttaatgaa
     5881 tgggatgact tcatttatca cataatcgtt aaattgattg tgacggatag ctttatgagc
     5941 tggttccata tcgttattga gccagctttc tttattgata ctattgatgg agtagatttt
     6001 acattttcca ctttctagaa atggcgcaag tgaatccatc atctgaaaac gctggtattc
     6061 aagaaaatcg gcagctgctg taggaattaa aagaatagaa aatccataat ggccatatat
     6121 agcaagaggc atctcggcat ttagagccgg actataccag gagaataatt cgtgtttcat
     6181 gaagatgggt tagcaaactt caataataag aaaggaaatt gattttattc taaaagattg
     6241 cccaatcatt caattgaacc attctagttc tagaaaccct atagagctat ttaatattct
     6301 ccgtttgaga gttagttctg aattgaaatt agattttcta gtgccgattc tagtttagtg
     6361 taattgaatt cgaaacctgc attcttgatt ttttgattcg aaattcgact tccttccaac
     6421 accacaacgc tcatttctcc taaaatcagt tttaacacaa aagaaggaac gcttataggc
     6481 aataatggac gattcatggt tttggctaag gctttagaaa aatcacgatt cgaacatgaa
     6541 gttgttgctg aagcgttata agcgccagtc caattagtat tcatgcatgc ttcagcaaat
     6601 acgcgtgcaa tatcttctat gtgaatccag gggatttgtt gcttacctga acctaatgga
     6661 gaacctgagt aatatttaaa cggtttcatc atctgtggca atgctcccga ttcgcgagct
     6721 aaaacaacac cagtccgaat ctttacaact cgattactaa tcgattcaaa agcatcagcc
     6781 gattgttccc aaagtcgaca cgtttcacct aagaaatcct cagctgcaga ttcaacttct
     6841 gcatgcaaat gattatcagt aatagctcca taccagccaa cagccgaagc gctaacataa
     6901 gcttccaatt gctggttacg ttttagtagt gctttcaaaa gcattgcagc cggtttaaca
     6961 cgactcgata aaatctcagc ctttcgctcg tcagtccagt gtttttcggc aattccagct
     7021 ccggctaaat gtataatatg cgatacacca tcgaatgctt gttcatctag ttcaccagat
     7081 tcgagattcc atagaaatac agcatctgca tgagcaacct gattggctga gcgactaaat
     7141 aaatgaattc gaaaaccctg ttgcttaaga ataggtaaaa tatgtgagcc gataagtccg
     7201 gtagcaccag tgaggagaac tgttttcata gaagttaaac tccacatggg caggaatgtt
     7261 cgataattca caagtagaat ttacctaatt cttgacgatt catttattta agtattttac
     7321 cctaatgtat tttacctaaa taaaaacctc cactggagtg aatcaattac tctttatcga
     7381 aacttttgtg cttggatcgg ctgtgattgc cgcaatctat cacttggtgt tgtttattca
     7441 gcaacgcgat aaattcttgc ttttttacag tttatacctt ttttcgcttt cggtatatat
     7501 tggcttcaag ctcattagca acaattacga tccgttttta cctaccgata atgtttggta
     7561 ttacatacta gaagaagttc ttcaggtggc gatggttacc gtatatgtat tattcgctgc
     7621 acaaacattg gaagttgttc aggaagagaa cattgtaagg tcgtttatgt atgcattttt
     7681 tgctttgtcg gctttttcca tttgttatca tgtgtttgat gcaatacaaa atggaccggg
     7741 tgtaaaatcg acttttgagt atgctttttc acgtatttca ttagtcggaa ttgcaacaat
     7801 tgcattgatt tcagcctggc ggatacgaac gactactttt caacgcacca tcattattgg
     7861 atctttagta tatgattctt cgggattgct tagtatcata tccttcaccc aaaaaacaga
     7921 tatcttagga ttgaatggtg tagaacctta tttagctggt tgtttattgg atattatcat
     7981 attctcgagt gcacttggtt accgattaaa aactattgct gttcaaaaaa atgaattact
     8041 cataaaagag tcacaagctc gcttagctat tgagcataca cgcactcaga ttgcggttaa
     8101 tttacatgat gatgtaggct caatcttaag cagtatgagc atttacagtg aagcagcgaa
     8161 acaatcggta cgagaaaaca atttaccgcg tgtaactgaa ttgattgata agattggaat
     8221 tagtgcacgc gaaacgatgg ggaatatgag tgatatcgta tggaccatca atcccattaa
     8281 tgataatgga attaaattat ttaataaaat ggaggatttc gcaagtccgc tatttcactc
     8341 gaaacaggtt aattttagct tttaggttgc ctcggaatta tttgatgttg attttccaat
     8401 gcctgttcga caacatgttt atttcatctt caaagaagcc ataaataatt gcattaaaca
     8461 cgcagaagca caacacgtta aagtgaacat tgacaaaatc caaaacgatt atgttatgcg
     8521 aattgaggat gatgggaaag gctttgataa tgaaaaagat tacaatggaa atggctataa
     8581 gagtatgaaa cagagggctt ctgagttaaa tgggactatt ttatttaagt cggccaaccc
     8641 gggaactata ttagttctca tgtttaagtt gtaataaaaa caagttcacc attttaggtg
     8701 atagttgtac aaatactaat tctctttatt tgaactctta aattgaatat tcctatggca
     8761 gttagagtga cgatatttga tgataatcag cgattgttag attcgctaga tgcattaatt
     8821 gggggaagtc ctggatttca aattgccgga attttttcgg attgttcaga tatacaatca
     8881 aaaattgaga aaagtcagcc ggatgtaatt ttaatggaca tagaaatgcc cgggataaat
     8941 gggattgagg cagttaaact ggtgaagcgg tattttcctc gaataaatgt attgatgcaa
     9001 actgcttttg aaaatgacga attggttttt gaagccatct gcgcaggagc tcagggatat
     9061 attttaaaga atacgcctcc agcgaggatt ttagagtgta ttgtggaagt ttatcaagga
     9121 ggaagcccga tgagtccaag tgtcgctaga aaagtgcttg gttttttaca gaggcctgat
     9181 aaaaaaacgg aggaaactat ttcccccaat aattacaatt tatctccgag agagaaagaa
     9241 gttttaggaa gtttagtgaa tggaatgagt tataaaatga tagcagatca acatggtatt
     9301 tcttatgaaa cggttcgttc gcatatgaag aacatttatg aaaagttgca tgtagcaagc
     9361 atgacagaag cagttgcaaa agctattaat cagaaattag tttaaaatta atcagtagta
     9421 ggctatcccc ctttttaggg atttacattg gggtctattt tattgaattt tgaattcaat
     9481 aatctaaata tcctatgaaa aacctatttg ccctttttct aattctcttt tatcacgttt
     9541 ccttttctca aacactcatt accgacacct tatttggtga aagtggatat gtatccgctt
     9601 tgaatgtggg taattcagag gtcatgacca ttctacaaca gcctgataat aaaattatag
     9661 taggtggaca taattatgac ttcggttgta attgtcatta tatcgacatg attcgcacgg
     9721 atgcatgcgg tatgatagat agtacatttg gaattaatgg tatagtccat tacacctttg
     9781 aacaaagaaa tttaggtttt aattataagc tacagcccga tggtaaaatc ttggttgttg
     9841 gtgtacaatc ggatggaaat aatggctctc agcagtatcc atttattgct cgttttaact
     9901 caaacggcac acctgataca acatttggag atcatggtag caggaaaatc aattccatag
     9961 gagcatgtga atttttaaat gtatatctat tagatgaagg aaaactgctt tgtacgggag
    10021 gtccaacaaa ttactcaaac gtaataatgc aatttgattc aacaggaaat gtggatccaa
    10081 cttttggtaa caatggagtt gtacaacata gtatacctgc aggaattaat ttcttcaata
    10141 gtttcttcag tatgatgaga accgatggta aaatcatttc cgttgcacca gcgtggatgg
    10201 gaggtgattt tagacaagca gtattaagtt gctttgatac acaaggtgaa ctagatacca
    10261 catttggaaa ccaagggttc tttatcgatt acgactttta tattgcaaat ggttttaaat
    10321 acgctattca aagtgatgac aaagtaataa ttgcccagca aaacactact gaaacagcta
    10381 tgaatattcg tagatataat acaaacggaa gtgttgatac cacatacgga aacggaggaa
    10441 cagtatcaat tccagcacct aacagtcctt caagaattca atttcttaca ctattagcga
    10501 atgatgatgt aattattggt tatcaagaaa atggagatgt gcccaaatac aaaaaagttg
    10561 atgttaacgg attagaagtg actgatttca ccttgaacgg tggaagctca tttcaatttc
    10621 caacaaacga tgataaagga agggttgctc ttactacctc aagtggtgaa atggtgatag
    10681 ctggtagtca aaactctttt tcccttacac gttttattgc aaatacttct attcccaaca
    10741 ttactatttc gggtgttaca ctaaattcaa atgtggggaa tttagaatcc acatttcaat
    10801 ggtttctaaa tgggaattta attgctaacg aaacatcaag ttctattgaa attagtcaga
    10861 atggtatgta tactgttgaa gttacaaacg cctggggctg taacacatcc gatcaacttg
    10921 aagttataaa tcttggggaa gagtcaataa gctcaattaa attttcggtc ttccctaatc
    10981 ctgcggagca aatactaaca attcaatcaa aagataatac tcttatttca attcgaattt
    11041 tcgacgtttc agggaaatta attaaatcag aaacatctaa tgcaaccttt actaatatta
    11101 aggtagatga gtttacaagt ggtttgtatt tcttggaaat aaaatctgaa aatcaaatta
    11161 cccaaactaa attcatcaag aaataaagga attaatttca ttccattata ataagcacga
    11221 cgtttaaaaa tgtcgtgctt attttattta atacatgctt taatctttat tcctccccag
    11281 tttaggggat atctattata gtttttaaga ctgaattttg aaaataaaaa tcatgaaaaa
    11341 aataattctg tatttattcc ttataaatgc acctcaagta ttttcccaag cacttgtcca
    11401 ggatcaactt tttggtacaa atgctgaatc cttttcactt cctacaggaa ccacgagtga
    11461 aacaaatgtt ctgtttcaat atccggataa aaaggtagtt gcttgtggtt atacctacga
    11521 tatcggatgt aattgtttct acaatattat gtttcgagta gatgcctgcg gtcaaataga
    11581 ttcctctttt ggagtaaatg gtttagttcg acacacgttt gaccaacgaa atgcaggtta
    11641 tggctatacg ctattaccaa atggaaaaat gattgttgtc ggtatgcaaa gcgatggcaa
    11701 ttcgggttct cagcaatttc catttattgc gcgttacttc tataatggac agcccgatac
    11761 cacttttggt gatcatggaa caagaaaaat tagcaccata ggacctgccg atttaaccag
    11821 tgtttatcaa ttagacaccg gtaaactttt atgcaccaat ggaccattaa tgatgcggtt
    11881 cgacagttta ggtgtattag atccaacctt tggcaacaat gggattttaa ttcaatcggt
    11941 tccttcacca tttaatttct attatcagtc gaacagtgtg atgcgaagtg atgggaaaat
    12001 tatttctgtc tcgagtgtat atactggagt tgataatgat aaatatgtaa cacaaatgtg
    12061 ttatgatgca gaaggattaa ttgactctag ctttggagta aatgggttta gtattgatta
    12121 caattttgtg gttggttctg caccacctag gttgatttta caaagtgacg ataaagtaat
    12181 tgccatccgc cagaatataa cagaaacggc cattataata gctcgatata acactaatgg
    12241 ttcattagat acctcatttg gctccgatgg ttatttaagt cttcctagca atcgattggt
    12301 atatgtttcg aattttaatg atgattcatt tttagttggg gtttcacaag gtggagttcc
    12361 cattcaatac ttccgtgtat ctgcaaacgg tattgttgat ccaagtttta ccctgaatgg
    12421 aagtgtttac tttcaaacga tggggaacaa tggtccttca ccgcaagcag gattggcttt
    12481 tggaaataat gaaatcgtga tggcaggatg caacagcggc ggaaatggat ctacagcaat
    12541 tgcgtttaca aaatttgctt tgacctccgg aattgcaact ataacacaaa tcgataacac
    12601 cctgaatgcg aatcttccag aagatcagtt tacgtttcaa tggtatcgtg atggtgtttt
    12661 agttgcgggt gatactacag cagctattac cgttttccaa aatggagaat atcgggttgt
    12721 tgcaagcaat ttagctggtt gtagtagtac tgatacaatc actgttttat caactggtgg
    12781 tcagacactt cgtcaagctt cggatgtaaa aatatatcca aatccaagta ctggttattt
    12841 tcagttgaaa tttaattcag aaataaaatc aggtattata tcggtaattg atatttcagg
    12901 gaaactaatt cttgaaaaac aattcaatca ttcctccaca ttaaatgtcg atttgacaag
    12961 cctttcgaag ggaatttatt ttttaaaaat aaatactgat aatggaatac aaattcacaa
    13021 aataattatt caataaatct aatccatgag aatatttctc ttttttattg ctatttgctt
    13081 gacaaccgga ttaaatgctc aattcactag tgacagtttg tttggtataa atggaacagt
    13141 aagtgcaaca caagtgggag ctaaggctac tgcaatactt agacaaacgg acgataaaat
    13201 aattgtttgc ggaaatctcg attataattt caacgatgcc aatcaaatgt ttagagttac
    13261 ggcatgtgga gagattgatt caagctttgg agtaaacggt tctgtgcagc atacatttga
    13321 atttcaaaac ttaggttttg attatgtatt acagcctgat gggaaaatcc tttgttgcgg
    13381 aattcaatcc tccgacaatg caggttcaca gcagttccct tttattgccc gctataatag
    13441 tgatggctct gttgacacga catttggaac tcatggcagt acaaagattt ccaattatgg
    13501 atctaattca tttggaaata tttttttgat gccagatggt aaaattattt gcagcagtgg
    13561 tccatttgcc atgcgcttct tagctgacgg aagcgttgac ttctcctttg gaaacaatgg
    13621 catcttatca atgcctacag ctcctaatgg ttattatgaa tcgcgaggct taagtaatgt
    13681 tttaagatca gatggaatta ttgtaggagc gaacaccgtt ttggacttta atttcagtta
    13741 cccatcatta ggagttgttg cttacgatac atcaggtgtt gttgacagca cttttggaaa
    13801 taatggttac ttgcaagatt ttgcaggggg aatagcagct tattcggtaa gaaccatatt
    13861 acaaagtgat aataaagtaa tcatcgcagg aaacaatcct aatggcattc acattactcg
    13921 agtaaccaca tcaggacaaa tagataccac ctttggaata tctggttatt tggatttcag
    13981 cccgggagga gaattatttg ccttaattaa attaaacaac aatcattttt ttgtaggata
    14041 tcgcttagca gggacaggga atttctttcg agtgtttgat agtaacggtg ttttagatac
    14101 cgccattagc ctcaatggca gcaataacaa catgggtgat ttgggtatcc tcaatgttgc
    14161 gcttgttgaa tcaaatggtg atataacttt tggtggcact tccttaggtt ttccttatcg
    14221 gatttctagg ttggtaaaat catctgcaat cccaactatt actctaaatg gagacaccct
    14281 tttttcgaat gtagcagata atagttgtac ctatcagtgg tatatgaatg gcaatctgat
    14341 tgaaggggcc aataacagca actattccat tacagctaat ggtaattatt ccgttcaggt
    14401 agcgaatatc tggggttgtt cgatatctga tgaatttaat gtgacaactg ttggtattga
    14461 tagagtggaa gattattcag tctccgtttt tccaatacca gcaacgaata cgctaacaat
    14521 tcagtattcg aaacccctca tgcaagcctc cctatttata actgatttag cagggaaaat
    14581 tgttttagaa aaacgcgaaa acaacacctc aacgaccttg cttaatatcg ctggttttgc
    14641 caaaggaatt tatatcctac agataacttc agatcagggt aattccagcc ataaaattat
    14701 tcttgagtaa ttctagccct tcctatttga acaaatttat ttccgtgttg atttgaatta
    14761 acttatctaa ttcgaataag ggagcataaa ttcagaatat tcaaaacctt tttaaataat
    14821 gaattaaaat gaaaaaaata aaatacacat ttcttttact cgctattttt agtttgagta
    14881 tatctgtcac atcacaaaca tttcagtggg ctgctcaata tggagccaca ttaaactctg
    14941 atggaaaagc catagcaact gattctgatg gtaatgtgta tactacagga agctttttag
    15001 gcacagcaga tttaaacccg ggatcaggaa catttaattt aacctctgca ggtcgagata
    15061 ttttcatttc taaattggat ccaacaggta attttatctg ggcaaagaag ttgggcggtt
    15121 ctagtattga tgatgtgtat gctatagcat tagatgatgc aggtaatgtt tatacaacgg
    15181 gtactttttc tggtacagca gactttgatc caggtacagg tacatttaat ttgactggag
    15241 gagctcaaaa ctatggagtc tttatttcga aattagaccc ctcaggaaat tttgtttggg
    15301 ctaaaaaaat tgaattaaca gacggcggaa atagttttga gaatacgcct tcatctatta
    15361 aagtagatgc tataggaaat gtcttaatca tcggtaactt taatatccca gcagattttg
    15421 acccagacga aggtgttttt cttttagatc cagacattac aacaagtgcc atttcggata
    15481 tttttattct taaactagac gcttcgggta attttgtttg ggctaagcag atgggtggaa
    15541 cttctttcga ttatggaaat tccatctcaa ccgatgcgga tggcaatgta tacactactg
    15601 gatattttaa tgccacagcc gactttgacc caggtgtggc tacttttaat tttacctcgg
    15661 tgagtaacga tatatttata tcaaaattag atgcagcggg taattttgtt tgggctaagc
    15721 aaatatcagg accaggagcc gacagaggca ctgagttaac tattgacgaa tcaggcaatt
    15781 gttatgttgc cggttttttt agcggtgtaa cagactttga cccgggaatt gaagtggtta
    15841 atttaacttc agcaggattg tatgatgttt tcattttaaa attagattca tcaggagatt
    15901 ttctttgggc aaaaaatata ggaggggctg gttatgatga agtatacgca cttgccatag
    15961 acgattcaaa taatgtttac accaccggta tttttaatta ttcggccgac tttaatcctg
    16021 gttcaggtgt atttaattta acttcagcag atgttgaaga tatattcatt tctaaattag
    16081 attcttccgg aaattttata ggcgctaaga gaattggtgg ccccgactct caaaaggcta
    16141 attcgcttgt gttagataac acaggtaaaa tatgtcttac gggatatttt ctggggggag
    16201 gaaccgattt tgatccggat gagggagagt ttatttttac ttcgaatgta aacagaactg
    16261 cttttacatt aaaattaaat gatttttcat ccagctgttc ctcaagttcc aattcaatat
    16321 ttcaatcctc atgtggaacc tatctgttta ataatcaact attaacagaa agcggaacct
    16381 atactgatac tttggtaaat ggagcgggct gcgacagtat cctaacgtta aacttaacta
    16441 ttaatgcaat tgataatacg gttgtacaag atgggaatac gttaacagcg aatggatctg
    16501 ggacgtatca atgggttgat tgcaattcta atttttctgc aatttcaggc gaaacaaatc
    16561 caagctttac gatttcctct tccggcagct ttgctgttat cgtaaataac gaaggatgca
    16621 tagacacctc tgcctgtttt ttggctttag gaataattga ttttaatcac ctccagagtg
    16681 tatctgttta tccgatgcct gtttctaata tgctaaccgt agaattttcg aagaaattaa
    16741 cgaacgcctc aattgtgtta attgatttag cgggggaaaa ggtttttgaa gggaaacaaa
    16801 gtaacacctc agtgattgtg ctggatatta gcggattagc gtcggggatt tatgtgcttc
    16861 atgtaaattc tgatcaaggt aattcaacta agaaaattgt cattcaataa atggaaattt
    16921 cagattgatg acaaatccga ttgcttgaga atatcagatt catccaaatt caacttaaac
    16981 tttgtcgaca gattcaaatt ataattcaag tgaaatgaac tccaatgtta attatcttaa
    17041 attgttattg tccaggcttt tgatggcatt atatggtgca atttttcttg caattcaata
    17101 aatatttact tgaattctga taaaataatt caacgtttta gtagcgattt aacttaacaa
    17161 aatagtggtt tgtgatttgt gttgcacaga ttaattctca ggattagtct gtgctttttt
    17221 aatttaaact caccattaaa tttaattatg attgaggtat aaaaattagc acatcacatt
    17281 tttgctcctt agttaactag cgtcgatgta aataaaatct tgttctcgag gcttttgaga
    17341 tatagaataa aaaaggtgga agcctgctga tctccacctt aaatcttact tatatatgaa
    17401 cttctatcca agatttgaac cctaataagg gatcaatatc aagtcttata cgtgttatag
    17461 atattattcc ttgacgaatt gtaccgttga ttttctacca tcggcagtgt ttaactcaag
    17521 aatgtaaact ccgggactgg ttaaatgcga aatatctagc gtgaagttat ttatcccttg
    17581 aaataaaagt tgtttttcaa tataggttct gatcaatttt ccacttacat caaacatttt
    17641 aattgaccct atttctgcgt ttggaagttc aaatgatagt tgaagtatgt cttgagcagg
    17701 atttggataa acgcttgaat tgactacaaa agctttttcg gaaatactac taatcaattc
    17761 atcgggcaat atacgagcaa tcaaaacagt ctgcatattg tcgttttgat tcgttccaat
    17821 gccacttata acaattttat catctgattg tattgccata gcatgaattg catcaccgaa
    17881 tgtcatgttc aattgaatga gaccatctac accaaatgac tgatcaggag tgccatcgga
    17941 gttcaatttt ttgatcataa aaaagctatt ctgagctggg ggtaattgcg gtgtagcaaa
    18001 ggcaactaca atttctccat ccgatttaat ttcaactcca taaggtagct cttcatgaat
    18061 tggattatat tcaattttta ccaagccatt agcgccaaaa ctcgggtcta tttcaccctg
    18121 ctcactaatt cggataacac tagtgcaata acctcctgtg gcaagccctg cataatagag
    18181 accagaacaa actattttac catcgccttg aaatgaaatg tacatcaatg agccataaag
    18241 tggccccgca tcgagtgtca caactccacc atttccgaac gaattatcca taactccatt
    18301 ttggtcaaag cgcatgatta aataatcatt ctcagataag ctaggttgaa caagcaaatc
    18361 accacagact acaatcatcc catcaggctg aattttaacc cccataattc cattatatac
    18421 ttcttgtgga taaggataaa aaacaccatt gataccaaac gttaaatcca actgcccatt
    18481 gatatctaaa cgtaccgtgg ttcccctgtc atatccagtt gtattagttt cgcccaccag
    18541 cagcaactta tcgtcagatt gcacggcgct gtcataggtc ttcaagggta cgaattcagc
    18601 agagccaatt aatgccgcgc ctgaaactcc gtaggacgta tccaatgtgc cgtcctgaag
    18661 aaaacgcaca gcaaaccact tcgtaggatt gatatctccc tctgagatat acatatttcc
    18721 tcctgcaatc aacttaccat ccgattgcaa ggcgactgat gtaacacgtt ccaccatacc
    18781 ttgttggtca taagacacaa atcctccagt gccaaatgat tcatcaagtg atccgtcaac
    18841 attatatcta accactacta aatctcttcc cgatgaggta aaaccttcac acacgacaac
    18901 cgacttttga tctggctgaa caagcatatc gtatgcaatg agcattggat aatctgcaga
    18961 tatagaaaga ttaaatcctg tattgttgaa ggtatgatca agatatccgc tttgtgaaaa
    19021 gcaaaaattt gatgtcagta ctatcgccaa aagactcagg caagatttcg aaatcatatt
    19081 actagttaac ctgttcataa tgaaatgttt tgatgaatca cgagaattaa atacatggaa
    19141 cttgtattaa aaatgtgatt gataagaacc ctatttaata atgattgaag ttgaatgggt
    19201 ttggtcttca gaaacaagaa caagtttgta catcccaggg actatctttg aatttacctc
    19261 aaatggaaca gtgtgttctc cacaattcat tgctttgtcg aggaaaatgg tttcaacaag
    19321 ttgtccctta aaatcaacta ggaagagtga tcgtagacct ttgcttttta gtgaaatatt
    19381 gacataagcc ctttcgtcca atggattggg aaataatgcc aatgaagttt gtacatcaag
    19441 cttatccatg ttcacggttg acgaaggcaa taatcgcaca agagaacctt tgtaacctcc
    19501 tgtatcagca gcaattccgg ccaagaggat tttaccatca tcttgaaatt caacatgaga
    19561 acacacctca tcattcgatg ttaaatcggt gaagtggagt ccattcgttc caaaagttgg
    19621 gtcaagtgtt ccatcttctt tcaattgaac caaggtagcc tttgaaggga aatcattaaa
    19681 tggggtagca gttccttgtc ctgcaagaag tattttacca ttcgaattga gtttcatgtc
    19741 ccatagtaaa tcattgtgta gtccacccaa ttcatccatt ctgaagattc catccgagct
    19801 gaaggtattg tcaagattac cattcgcatc caaacgaata cacatgaaaa cactattgct
    19861 cgagttggta ttgttattcc ctgccgctaa aattttacca tcgggctgta cttcaacacc
    19921 aaaaaactct gctaataagg gcgcttggtt taaaataaat attccagcat cagcaaaagt
    19981 aggatctaat tgcccatcag gtaaaaagcg aaaaatagta gcgggagatt catcagaact
    20041 gataattaat gtcccagcag ctagtatttt cccgtcttct tgcaatgtca aacatcttcc
    20101 cgtttgtagt tgctgattgt aatcttgaat aactaatcca ttgtttccga atgttacatc
    20161 gggagtaccg tcggtattgt atctcgccat taccaaatca gtttggccta aatagtaaga
    20221 aatgcccact tgaatcgact tcccatcagg ttgtattacc atttcttctg gaataaatgc
    20281 ttgtccttca aaaggaataa taacagacat tccgtttatg ccataagtta aatcaggagt
    20341 tccatcgtca ttatatacag ccataaaggc gtaattggtg taaaatggat tgttttcgta
    20401 aagcctatat gttcccgata cggcaatttt cccatcaggt ctaagacgaa tggtgcgagc
    20461 aaatgttgca gcttctgcca gattaacccg cacgactcca tctattccaa atgtactatc
    20521 cacaacccca tccgaatgat aacggataag taagagctca tttccctgaa atgaaggaga
    20581 ggaaatagta atgttagttc ctcctgctgc aatgattttt ccatcgtttt gaatgacaca
    20641 agaatagaat tgagcttcat tacctaaacc ttcgggagtt ataactaatc cactctgatt
    20701 aaagcttaaa tcaaccaatt cattttgtcc gaaagccctt gatgaaatta tcaataatgc
    20761 taaaaaagaa ttcaaatagc gaaatctaga aactcctgcg ttcaatcgat gtgaagtaaa
    20821 gagaaagctt cttatccatt tactattccc gaatttttcc atttcaaatc tgtttttatt
    20881 cttattagat gagggaaata atctctcgaa ataaacaaga cataataatt tagtttaatg
    20941 ccaaagatta gctattcaac tattggcatc aataacctat gttggtgatt ttagtagatt
    21001 gtctctcacc aattatggtg gtttttcaat tattcattag ctattaacta ttcgcataaa
    21061 tcgatatgat aaagctgaat tgctgaattg actaaaaaac ctatgaaata taattacagg
    21121 ggttatccta ataaaacacc atttccatct tgatttctat gtgtttagat gagattgtgt
    21181 ttattagagg atttttaata ttcttcttta aattaactaa ggaacctgaa ccactttctt
    21241 ttccgacatg ggagattctc ctccatcggt ataaataact tttacagcat agctatattc
    21301 tcctgataaa gagacagttt tatcttcaaa gctgcggtcg gttcctttca acattttgta
    21361 tttggtgagt ggttttcctg gcatagcccg ataaattaaa aaggaaaaat ctcctgaatt
    21421 cttgtatttc caagttaaat ccgttgactt tttctcttta ttaaattcaa ccgttaattc
    21481 tgcaattctt tctcgcaaac ctgaatcata tgtgcgaata gagactagtg aagagctatc
    21541 agactgcaaa tcactactat cgactgcaat taacaggtat tcaaaaaact gtttcttagg
    21601 aagatggcgg tcggtaaaag atgtttctgc gtctagaaat ttccagcgtt ttaattccaa
    21661 ccaaacggaa tctttagctg gttttcgata aagaatctgc tctttcacat catcactgct
    21721 actgttaacc caattcaaat gtacggttgt atcagtcacg cttaccttac caaatacagg
    21781 tgctacagga ggaataacat ccaatcggcg gactttgatc caatcagaat aggccgagtg
    21841 attatagtta ttatcgactg ctacaatttt gtaataaatg tatttagtca aggtgcgttt
    21901 ggtaattgta tctaagaaaa tagtgtcgcc ccaaatggca ggtgtaatat tgctgaattc
    21961 gtgatcagaa gcatttgcta cataaacccg atagccttgt aagtcgggct ctttccctaa
    22021 attccaagtc agatggacaa ccgaagaaga atcaatataa ccagctaatc caattggagt
    22081 ttctggcgca taattatctt ccacaattcc ataaaatggg agcgatttaa tttcatttcc
    22141 agccgtgtct ttactaatta ccacataaaa attacctcca tttggattgg gaattgaatc
    22201 taaaaatgag tctacatcct tagctaattc gccagatatg gttttgaatg ggccatccgt
    22261 atcatatccg cgcgcaacaa ataggctctt taaatcagga tcaatttgtt ggattttcca
    22321 tttaattcga attgttgttt tattctccga attaactcca gtaataacag cttgagaagg
    22381 tggcgttaaa tctcggccca tgactgtagt aacagccgaa ggagagctta attcaccaaa
    22441 agcggttact ccaataattc gataatcata taaaatataa ttctcaactg aatcggtgaa
    22501 ataagcatta ttttcttttg tgaattctgg tttaaaactg acaaatggat ttttatttaa
    22561 tcgaataaat tgtttcgttc ctgaaggagc acgttcgata tgaaaagcgc taaaaccact
    22621 attctgacta gcatcccaac gaagttcaac ttttctatct ccacctataa tggcaggtgc
    22681 tcgagcaatt tgtattttag ataattcact ggaattaata tatactaatg cggtgtcaga
    22741 aaataatcct tcaatggatt tagaataaaa tactcgatat aaataattct ttcctttttc
    22801 aaaagaacga tctacgtaac gaagtccaga agcatcggcc actttaggtg aattatcggc
    22861 tgcaaacaag gcaaagccat gtcgcgtttg aagcttattc tggttttgtt gaattaaaat
    22921 cggatcagga ttagcatagg gcgtaagttc aggatacatc tgggctgcag caatcatagc
    22981 cattgcatta tctttcccaa aattcttaag ccattcttct ttagctaagg gttttaatgg
    23041 agcatttgtt agtagaactt taactactgg tttacttgta tcatctggta tttcaacacg
    23101 ttcaacaatg aatccattag ctagttgcat ttcccaagta taagcatgat cgggtgccca
    23161 acggaggaca atgctatctg atttaacctg tgaaattatc tttatacgac acgtgtcaat
    23221 tcggataaac gtcgaatctt tttgagcaaa tgttgatgaa caaatgccca atagcaaaag
    23281 acttagaatc tgtatgttaa ttcgagttgc tttcatcaat ttgttcttag aaataatagg
    23341 gtcactactt gaggataatt gaaatacttg aatgttcctg acatggaaat agtgtatggt
    23401 ccataataca taaattgata aggagtattc agaaatgaag tgactttagt acgaaccgca
    23461 agtggaacac cagtacggtg ataatcattt gaatacgttt ctctttcatt tggatcgtaa
    23521 ccccttaaat tattcccaaa cctcgctttc atatcagcaa taatgacttt catcctttca
    23581 tagtccattt gagcataata ggatgttgtc aaaataagtt gttgttgcgg aacataatca
    23641 gcagtatgaa tatctgcaaa tgcgtatgga tcatttgcaa gtatatagct agtagaagaa
    23701 gagctatttg aagatgggta aatctcatca ttcgttattg gtcccttcat tcttatggtt
    23761 ccactaattc ggttgggagg caaaccaata tcatcgggag ttggtctata aatagacaag
    23821 tgattataaa cttgtgcatc acagatagca gaataggtat tgtatatttt gtccattaga
    23881 aatgagctgt gccatgaatt tgaataggag tctataatat ccatagacct cacaatattg
    23941 gttgtaaaac cgtccaaata cctgaagccg tctatttcaa acctttcaaa tccttcaggc
    24001 cctttgaaat tcagaattag attttcacga ggatcaacat attctctctc tacgttatcg
    24061 acttgtaagg aattgatttt tgctgatatc gttccaaatt cagacgttct gaaaaagtat
    24121 tcgtaaatca aatgttcatt tgaacgaaga actggggcac cttctaattg cctgtctcta
    24181 agaagtacag caccattgtt aaatgatatt ttatttaatg atactgtttg aaagcccgcg
    24241 ttttgtacca gtaaattacc agatgtagca gtagttccag agactctaat gtctctatga
    24301 ataatcctta gtctgtatat tgttttagga gccaaaagag ctggacttcc aattgaaata
    24361 aaacctaatg atcttggagt tcggttatcg acttcaaatg ttccattggt ataagaaaca
    24421 ggtgtttcaa ttatttgccc tccggcaaca ggctggaact gtgcataaaa tgaacgttca
    24481 taaccttgtc gggcattttt atcgaataaa ttaggaacat tggtgttcaa acgaattcca
    24541 agtcctcctt tatcaatgaa ataccgttga cggtttaatg gatatgagaa tttgatatat
    24601 tcttctctaa gaacatttgg aagctctcct gtttcgaaac tataaactaa acgttcggta
    24661 attaagcttc catcattcct tgtagcagga attacttcac catttggcaa atattcacgc
    24721 gcctgaacag ctataattat tctatattga ttgaaaggtg ataataaatt actcgtgtta
    24781 tgcatcgcct gagatccatc aggacttagt aaataatcat catatacatc taaaacagtt
    24841 ctgtttctgg ttcgatcaaa gagtgtaaat tcagtgatta caaacttgaa acgtcgtgtg
    24901 cgtttttcac cattggcccc tgtttcttgg aaagaaatca gtttgtcaat ttcaatattg
    24961 aatactacag tgggtttaac tccacaatca acattcgttt gagaaggatt cggacttaca
    25021 tccgaaatca gcacaaaccc tcctattggg ctttcgggtg gtggttcaca gaaatctcct
    25081 tgttgaactt caaaacgaca aaccccttca attaatcccc caagaatgct atagtttcca
    25141 cctagggttc ctttaaacca tttaggattg ggagtgccct gttgcaagag aacagctgca
    25201 tttacatcaa gaatagggta atgagtattg tcttcaaata aactcgcaac tacacctaag
    25261 cttcctctca tgtatcccca accagttcca gtagaatacc agccattaac tcccattttt
    25321 tcgttggcta acatgccatc gcatcgctcc gatccatgat caaaatgggt catgtcgaat
    25381 cctatctctg catcgagatt acagtagaaa ggagaatagt cctgattaat tttaggagga
    25441 ataaaagatg ccccaaaagc atatccttgt ccggtaccaa tttttgatgt tcgctgggaa
    25501 ggaaccattc caagtaaatc ggtcactttt gaaggaacag gagctggcgc aggtaaatcc
    25561 atacctgtca tcaaatatcc ttttgcatta aacactccac caatcaacaa tcccacttga
    25621 gccgtttgag gtgttggttc tcccaacttc aactgccatt taggcttact tatatacaaa
    25681 aaagcatttc ccgccatatt cttggcttga gtgcctttca aagtcattgg cacatccacc
    25741 ataactccaa aatttcctgt caatatctta tttgggaaat catattgaag aacaacatct
    25801 gctcgaacat tagtaaagct ccggtccatc acggaagtca taaaataccc acttccacta
    25861 aatgcaacct gactaagtcc accactatta ctgaatgttg ctcctaaagt aaggtcggca
    25921 ttaaatgctg ttcctcctcc agtcgtacca agtactacgg ttgctttcaa accaaatctt
    25981 gaactagcat caggtaccaa tctaacattc gacaaggtcg ttccaggcac tggcagttcg
    26041 cttgcagagg cattggttag cgtgcttgcc ggaggtggaa tactcatgaa tttcatcttg
    26101 taaaatgcgc caccaccaaa accataaatg tccataggta tttgataagg cgctattccc
    26161 ataggaattc ccggaacaaa cttcgccatc gcatctacat accaataacg catgttattg
    26221 atttctccaa aagaaccgat taccgatacg tttactgttt taataaatgt ggcatcaatt
    26281 cctccaataa aaccctttcc ataaagcgga tcttcggcat aaaaactcac aaacccctta
    26341 gcagaaacaa atcctaaatc accatcgatg tatatgcttt tgagatcgat accagttaag
    26401 ttcaaacttg gataaaccac tggctctccg tctgctgcag ttagcaaatt acctggattc
    26461 aagcgaccta aaatagccat cgttgtgcta ccgctgaaag agttcgcctc tccagtaaag
    26521 ttgacttcta ctgtaaattt taacgcaatg cgtgaaccgg gagtatcgtc ccaactaaag
    26581 aatccatgat cctgttgaac acctaattcg atctccccta ttgttactgg aaatcccgcc
    26641 attccctttt gtggactagc aaacgtaaaa gctgaacaag tcaagtaagg ttctgcggaa
    26701 gtaagtctga gagcttggaa ttgcaaccct ggaaaattta caggcacttt ccctatggtg
    26761 gttgtgatac tcacacttcc gttcaaattc aattcggggc ggaaattagt agcgtccaca
    26821 ttgacaatga ttcgtgaagt gggcaagagc tgaagttgag cagcccaaat aggaaccgta
    26881 agtgtatcta aggatcgcac attaaattga tatacaattc cagtagctat cgcaccttgt
    26941 gaaagaaccg attcgtactt taacaaagta ggagaaagtg gtgttttgat ccttccatta
    27001 aatcctccac ttacaaagct attagagaca aatctcaaac tcatcgtgtc gattgcaaac
    27061 gaccaatcac ccaataaacc attctccatt tctaaaactc gaagcgcttc taattttcca
    27121 gagattccag ttcggtcgat aatcaggtca ttaatcgcaa ctactacccg atcttttagc
    27181 acactatcgg caagggctac atcggtatca gggaaacgga ttttgaattt tttcggtaag
    27241 ccaacacgta aactttttaa atgaaaacca ttccacaaga cagaacgttc acccacataa
    27301 ttgtcgggat atacaatgtt ttcggggttt tcaaggtcgc taaaatcgag tgtggcttga
    27361 tcaactttga aagtccaatc atccactccc ggtatcataa atgggctaaa atcaagtgct
    27421 gctaaccaat ttcctgagcg gcgtacatta aatccaaaag atgccgttac cggcttgaga
    27481 gcaatgcttc cattggctgt agcaggcttg agaacggatt cgtcaaaagt aagtttccca
    27541 ctcaaatgca gaattctaaa tccttgacaa tcccagctta cataagtacc cgaatcggca
    27601 actacaccag ctgttacacg tgtattattt aaacggaaat
//
LOCUS       DAOOQW010000010        27322 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_2073_length_27322_cov_5.334030, whole genome shotgun sequence.
ACCESSION   DAOOQW010000010 DAOOQW010000000
VERSION     DAOOQW010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 27322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27322
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2073_length_27322_cov_5.334030"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1056)
                     /locus_tag="PK281_01430"
     CDS             complement(<1..1056)
                     /locus_tag="PK281_01430"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HOY48029.1"
                     /translation="MILALSGLIIIQIYWINNAIDLERQRFESTVNQALHNVVEMVER
                     QEVANKVRTRFDASKQGKMFFLGIDSLIRKSIDRKDTTTSGLVFWNEVSPGELQTEFR
                     QLNQDGSIEFVGETRTDSGGQVEYKKLRKARQSSDASSFGAITSDLNPLERRKDPRLE
                     RLMKKSGLVTDIFEELFDLNVNSGVEDRIDPILIDSLLKVELKTAGVNTPFEFGLYDF
                     INNKMFVDHPTDYTEQLMKTRFRVRLFPHDIFYHPDYLMIYFPEQNRYVFNNLWVMFA
                     SSGFFILIIIATFFYTISTIIRQKKLSDIKNDFINNMTHELKTPISTISLACEALNDP
                     DVNKSASMAGNYTRMIQE"
     gene            complement(1092..1874)
                     /gene="rsmA"
                     /locus_tag="PK281_01435"
     CDS             complement(1092..1874)
                     /gene="rsmA"
                     /locus_tag="PK281_01435"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013997669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HOY48030.1"
                     /translation="MALFRKPVTAKKALGQHFLKDLSIASQIADSIPAMDSTRKVIEV
                     GPGMGVLTNFLLEKNFDLKVVEIDRESVKYLKANVPALEGRIIEGDFLRMNLDQYLDH
                     TGAVIGNFPYNISSQILFQCLDQKEHVQIIVGMFQKEVAERVAAKHGNKTYGILSVLL
                     QTYFECEYLFTVHEHVFSPPPKVKSAVIRLTRKENVELPVPYPFLRNVVKTGFNQRRK
                     TLRNALKPIIAGREFQHPFLDLRAEQLSIQDFADLSVALINL"
     gene            complement(1874..2176)
                     /locus_tag="PK281_01440"
     CDS             complement(1874..2176)
                     /locus_tag="PK281_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4286 family protein"
                     /protein_id="HOY48031.1"
                     /translation="MILYNVTVNIDPELHLEWVNWMKSTHIPEVLSTGLFVENRFFRV
                     LNTEEGEGFTYSVQYFLDNMAKYEEYKELHAPRLQADFASRYKDRFVAFRTLLETV"
     gene            2311..3309
                     /locus_tag="PK281_01445"
     CDS             2311..3309
                     /locus_tag="PK281_01445"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY48032.1"
                     /translation="MKHIVLFALSCIALNGYADPGDTLGVSLGSTPSFSPANQVTLPP
                     YQGGYAFGVNYDPNNNFIGIGQGYINEEPANIVGVLSLIGAKSKGVNNVPNTKLTFGL
                     HNMSNSGAFNIITTTNPPSLEATNGPSVTLLGSKSLFFEEIDTTLGAYNYVAFDDTIA
                     VNGNLVVSVDLAELKTAGDIVGFYSDVPGNAFGINFAFHKANVAGDIMWFTSNSVFQG
                     ALDVSLAIFPVIAADVPDAILETSKAFQGVRALAMPNPANDVLNIQLEISETANYTVQ
                     LVNVQGQLVKELSLGVKTPGVHTVETTVAELESGTYFYSIISDTGIRFSKTIVLAH"
     gene            complement(3363..5222)
                     /locus_tag="PK281_01450"
     CDS             complement(3363..5222)
                     /locus_tag="PK281_01450"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOY48033.1"
                     /translation="MSFLRYLFFILLLSPFVLLRAQPGNDAPGQGLPSTEEQLAGQYY
                     TEGEWQKAADVYEKLFEQAPAGFYYSQLVSCYLNLKDIKSAEKLVSKQIRKNPRQLTF
                     LIDLAYVYQVSGEEDKAKKQYEKALKELEPGDDRQVTDLANALQQRKQIPMAIRVYEL
                     ASRIPRAYNFNLELAALYVQQGETGKMLEQYMNLLNTTGGEYLEQIQGSLQDMMASDV
                     SGEKTELLRERFLKEVQRNPDNLVFSDLLVWMFVQKKDFESAFIQAKAIDKRLKERGE
                     RVYNLARICMSNDAFETGKKAFQYVIEKGPISSLYVMAKKELSVDMYRKLSTSSSYSP
                     AELQELESLLTETYRELGIGEQSYAVAIRLAHLKAFYKNQPQEALAILEPLTQANSGL
                     SNRSMNEVKLEVADIQLLTGDVWESTLTYSQVEKSMKTDTLGQEAKFRNARLAYYKGE
                     FDWAKAQLDVLKAATSKLIANDALELSLLIGDNLVFDTTGEGLRMFSRADLWIYQGKL
                     SAALTTLDSLEEAFPASSLSDDILYRKANIAIRQSRFADAESLLDKIVTTYQKDILAD
                     NALFKLAELNEKQLGNQAKAMELYKELMTDYPGSLFVVEARKRFRTLRGDVIQ"
     gene            complement(5302..6474)
                     /locus_tag="PK281_01455"
     CDS             complement(5302..6474)
                     /locus_tag="PK281_01455"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HOY48034.1"
                     /translation="MNESLPLSVILNELGENREHYFNAVSPAIAQSSNFCYPSVSSMR
                     NALQKEFENPFYTRGYNPTVGILRQKLATLEGAEDALVCGSGSAAVSVAVIGNLKSGD
                     HVICVQKPYSWTYKLLDVLLARYGVETTFIDGSSISHFEQAKRPNTRMVFIESPNSMT
                     FELQDIRAVAAFAKENGYISIIDNSYSSPLFQQPHVMGIDLVVHSATKYLNGHSDVVA
                     GAICGSREMIQRLFASDWMTLGPAISPHDAWLLMRGLRTLDIRANRSADTAEFILPVL
                     EKHPKLSRVIWPFSDQHPQNELARKQMKRCAGMFSLVINTDSAAGVERFCDSLKHFLI
                     ACSWGGYESLVFPVCGLASTPSYDNPLMPWNLVRVYVGLEDPQLLLEDLLQALDQV"
     gene            6593..6925
                     /locus_tag="PK281_01460"
     CDS             6593..6925
                     /locus_tag="PK281_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48035.1"
                     /translation="MQHRGELLESAVRQSGIPLARITKKLNRSRRWLYNQFAKPDVSV
                     DVLMEVGKLIHHDFLPELHDLSLGKAARNISMDNTVTDYGEQNAQYWKDKYMHLLEEY
                     NTLLKKHQ"
     gene            6993..7829
                     /gene="tatC"
                     /locus_tag="PK281_01465"
     CDS             6993..7829
                     /gene="tatC"
                     /locus_tag="PK281_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HOY48036.1"
                     /translation="MALDQPQEETGHEMSFLDHLEALRWHLVRSAIAILAFGTLAFIY
                     KDFIFDGILLAPKYHDFPTYRFFCWLSQVLFHDDSICLGDLNFELISISMSGQFTAHI
                     TVAIVTGVILAFPYLLWEIWRFIKPALYQNERKYANGLVFWGTFLFLLGVAFGYYVIT
                     PLSVFFFGNYQVSTLVSNQIGLNSFISTVVTTTFGSGLVFELPIVIYFLAKIGLVGPE
                     FLRNTRKVSYVIILIVAAVITPPDVTSQIIVSIPLFILYEISIVIASRVEKNRLAELK
                     SR"
     gene            7886..8071
                     /locus_tag="PK281_01470"
     CDS             7886..8071
                     /locus_tag="PK281_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013996246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HOY48037.1"
                     /translation="MLSVLLTLLSPGHIILIVAAVLLLFGGKKIPELMKGIGQGIREF
                     KDAKEGKNDENLPEKKN"
     gene            8093..9526
                     /gene="gatA"
                     /locus_tag="PK281_01475"
     CDS             8093..9526
                     /gene="gatA"
                     /locus_tag="PK281_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HOY48038.1"
                     /translation="MYTSLAETQAAIAAGTTTIEAVTRHYLKTIAEKTDLNAFLEVFD
                     EDALKQAAAADQKWKSGNPGKLAGMVIGLKDNLCYKGHSVTASSKILEGFESLYTATV
                     VQRLIDEDAVIIGRLNCDEFAMGSSNENSAFGPVKNPFDPNRVPGGSSGGSAAAVAAG
                     LCLATLGSDTGGSIRQPASFCGVVGLKPTYGQVSRWGCIAYASSFDQVGPLANNVEDC
                     AIILEVIAGPDEFDSTMLQKPAPEFSKHLQAEGKFRIAYMPDYINNEGLDPEIRQYMN
                     SMIEQLRADGHTVNAVEFPYLDYVIPAYYVLTTAEASSNLSRYDGIHYGYRSKNAHDL
                     ESTYKLSRSEGFGKEVKRRIMLGTFVLSSGYYDAYYSKAQKVRRILRNRTNEIFAEND
                     FILLPSTPGTAFPLGDRSNDPIKMYLEDIYTVQANLCGIPAISLPLGKHSNGLPFGVQ
                     LLAPSFNDQQLLAFSARLMSDKAEPVR"
     gene            9688..11283
                     /locus_tag="PK281_01480"
     CDS             9688..11283
                     /locus_tag="PK281_01480"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOY48039.1"
                     /translation="MRLKVLFSGLFLLLASLAFPQKKTFVSARDTTVRLLADDPIAAQ
                     LDSLAYLKLFETTRYGGEFGRPGNFGFTPDSVPVYSPQVLAERIAKMDAQSPFKLIYN
                     DEVKAYINMYSIRKRNQVSRMIGLSELYFPLFEETLAKYKMPLELKYLAIVESALNPA
                     ARSRVGAQGLWQFMYGTGKLFKLNINSYVDERCDPIKSTEAACLYMKYLYSIYKDWSL
                     VLAAYNSGPGNVNKAIRRSGGKTNYWDLRPYLPRETAGYVPAFIAAVYVMNYSKEHNL
                     YPIMPKAIFSEIDTLHIHRRLTFNQLSAVLDMPVEDIAFLNPSYLLGVIPETEGHHIL
                     TLPKSKVATFINNEETIYSYKSPRESRMDSLMSIKRALPIASKTHVVRSGESISAVAR
                     KYGVTVNEIKKWNKLKSAKLKRGQRLKVSPPYTAQVEQSVSSSGGNTEIKPAEESKSN
                     TQATEEKTEQASESATPTETSKTTTKEKKTPGISTYKVRPGDTLWKVAVSHGMTLAEI
                     RKLNGFTSKTVLRTGMKIKVKTK"
     gene            complement(11570..11833)
                     /gene="rpmA"
                     /locus_tag="PK281_01485"
     CDS             complement(11570..11833)
                     /gene="rpmA"
                     /locus_tag="PK281_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009388778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOY48040.1"
                     /translation="MAHKKGAGSSKNGRESHSKRLGVKIFGGQLAIAGNIIIRQRGTK
                     HHPGLNVGIGKDHTLFALVDGTVEFKRRGDDKSFVSIVPFVAE"
     gene            complement(11849..12160)
                     /gene="rplU"
                     /locus_tag="PK281_01490"
     CDS             complement(11849..12160)
                     /gene="rplU"
                     /locus_tag="PK281_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010136145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HOY48041.1"
                     /translation="MYAIVDIAGQQFKVEKDQELFVHRLDNNEGDSVKFDNVLLIDNN
                     GSVKVGAPTVSGASVSAKVLTHLKGDKVIVFKKKRRKGYQKSNGHRQSFTKIQISGIN
                     A"
     gene            12375..13049
                     /locus_tag="PK281_01495"
     CDS             12375..13049
                     /locus_tag="PK281_01495"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HOY48042.1"
                     /translation="MTFFLALCGTGLFAQGNSGTLQGKITDDATGKGIEMAVVKLQAG
                     TQTFGGYTDVNGDYAIKGIPVGKYEVEISFVGYQGLKLSEVLIKNSSITFLNKTLALK
                     DGNGYEFIFEKWKDDLLGAGTPGTMKRLGPEDISFSANSRGVGDIIATSVPKVYMKDS
                     GGDLNFSGSRSGSTLYMIDGVKVIGEPQLPNQGIAEMVVITGGLPAQFGDTTSGVVLI
                     TTKSFH"
     gene            13163..14452
                     /gene="hemL"
                     /locus_tag="PK281_01500"
     CDS             13163..14452
                     /gene="hemL"
                     /locus_tag="PK281_01500"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013598980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HOY48043.1"
                     /translation="MNRDRSAALFETAKTYFPGGVNSPVRAFKSVGGTPVFIKKGDGC
                     RLWDEDGNEFIDFCFSWGPMILGHNNSKIREKVWDAIQNGTSFGAPTQLENELAHLIL
                     SNNRFIEKLRFVSSGTEAVMSAIRLARGVTGRDKILKFEGCYHGHVDSLLVKAGSGLI
                     TFGNSSSAGVPKGLVNDTIVVPLNRIDLVEKAFEEYADQLAAVIIEPIPANNGLLQVS
                     ADFLRALRTITQKNKALLIFDEVISGFRVGFEGAAGLYDISPDIITYGKIIGGGFPVG
                     AYGASKEIMGRVSPDGDVYQAGTLSGNPVAMAGGIAALTECLKPGFYEQLQLKTERFT
                     AKILQHTQERNYPFRIFQTGSIFWLSFASEDIHAAEEIDPNGMSKFKVLHAFLLENGV
                     YFGPSGYEVGFISSAHNEQDLEYAAQKICEGLDLVYS"
     gene            complement(14449..15333)
                     /locus_tag="PK281_01505"
     CDS             complement(14449..15333)
                     /locus_tag="PK281_01505"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HOY48044.1"
                     /translation="MKPSAWLSEWIRLVVFGNIWVAFGAASMYGCTTRLHNLPFSPLL
                     FISIFGATLFFYNYHRLFRKEVIYALQVSDRHQWIIDNHRKLQVFALVGLLMGIGGIY
                     PFLTLSFLARFSPFILLAVFYVIPVWKENGHWFRLRDLPYIKIFLVSAVWAFVTVFLP
                     FMTQSNTWLPDTSVWLTTIQRFIFIFAITLPFDIRDLEHDRKNQVKTFASLWGIPKLK
                     FISRSLLVLTTVLGFVSFQLGFYTISLSVALLINCASTGYLISQADEGLDEWFYAGWL
                     DGAMLDQFFWILLLGSLF"
     gene            complement(15335..15952)
                     /locus_tag="PK281_01510"
     CDS             complement(15335..15952)
                     /locus_tag="PK281_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48045.1"
                     /translation="MMPTWKSFWFLYFCLILSSSNLFGQDTLVLIDRKPILISSSRVE
                     NDYVYYVKAGRKREKKIEQDKVYSHRSKGKPDQIYYKQDTLENNWYTAEQMAFYINGQ
                     VDARNGYLKQARKTGSGGMMFGFAGAAAGMFYGPLFVIGYTAWKGYTLPKFTAENGYN
                     QAYIDNPYYKEGFGTTAKRMVTKRSAAGSAAGYILGVVTLTLVLN"
     gene            16107..18977
                     /gene="gcvP"
                     /locus_tag="PK281_01515"
     CDS             16107..18977
                     /gene="gcvP"
                     /locus_tag="PK281_01515"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HOY48046.1"
                     /translation="MIRDFESRHIGPNEAETAKMLQVIGVNSLDELIDKTVPASIRRT
                     DKMQIAPALSEFEYLQHIRQIAAKNKVFKTYIGLGYYNVITPSVIQRNIFENPGWYTA
                     YTPYQAEIAQGRLEALLNYQTMVMDLTGMELANASLLDEATAAAEAMHMFYAMRNRDQ
                     VKRNANVLFVSDKCFPQTIELLQTRSAPIGIELQIGNASELVWNDQIFGAVIQYPDVD
                     GEINDYREFTAAAKSNHSFVCVCADLMSLVLLTPPGEWGADCVVGSTQRFGVPMGYGG
                     PHAAYFATHEEFKRSIPGRIIGVSIDSHGNPALRMALQTREQHIKRDKATSNICTAQA
                     LLAIMASMYAVYHGPDGLKRIAGKIHSATRHLATRLSSLGYTVKTQAYFDTIRIGLNN
                     LCSQELLNQRAIQREINLRYESNGDVSLTIDETTSQVDIKALLDLFAGAAGKDAGSID
                     SYSTIPFWENFSRSSAILKERVFNSYHSETEMMRYIKKLENKDLALNHSMISLGSCTM
                     KLNAASELMPLSLPEFANLHPFIPSDQAEGYLEIFRELSSDLCQSTGFAAVSLQPNSG
                     AQGEYAGLMTIRAYHLSRGDGHRNVALIPSSAHGTNPASAVMAGMEVVVVKCDENGNV
                     DISDLKAKAAQYRDQLSALMVTYPSTHGVFEEGIREITSIIHEHGGLVYMDGANMNAQ
                     VGLTSPGTIGADVCHLNLHKTFAIPHGGGGPGMGPIGVNSKLAPFLPGHGLVKTGGDQ
                     AITAVSASPFGSSLILLVSYAYIKMLGPDGLTNSTRYAILNANYLKAKLEKYYPVLYK
                     GSHGFVAHEMILDCREFKRTGHVEVGDIAKRLMDYGYHAPTISFPVPGTIMIEPTESE
                     GIAELDQFAEAMISIRNEVAEIEAGKADPENNVIVNAPHTAVSVISDSWNKPYSREKA
                     AYPLSWVADNKFWPSVGKVDNAYGDRNLVCSCAPIESYAQPA"
     gene            19173..20993
                     /locus_tag="PK281_01520"
     CDS             19173..20993
                     /locus_tag="PK281_01520"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY48047.1"
                     /translation="MNSKLLLAMFLGASATLSAQNASTQSVPANTTKKNNYVHQHAAP
                     ASNSFSNRVDIWTNDFSNAADWVLTNEVGNNSNWVIGTAVPAGNFPITAIASTSAANG
                     YALFDSDLDCSGNQVPNLTTANSIDCSLFPAVVLEFEQQYRRFDDSTFVFVSNDGGTN
                     WVKYTVNGTLTNNDATATNPDVAKVNITATAGGQANVKIRFQFWSPATYTTGIGGAPG
                     CAYAWMIDDVKLVTPPDYELLVSTVYHGDPYNNFEYGTYPLSQVDTVRVVAIVSNEGG
                     QTQQATVNYTVTRGATTVNNGSSATFDITPFTSDTIIVSTGYVPDAVGNYSVAATITS
                     ANEDFDPSNNSGSSDFVVSDYIYSGLTGLAGTSALTFSGAGPTFDASKIAHQFIMINN
                     ASLKAIDIAVSSLSDDVDLIIELFDAADLTVALGVGDFSITSSHPTTAQYYSIIIDEQ
                     SLTAGNVYVASVGSEATDKKFVFYGVDGDPDDATLVNVPDDNGVLTWFRTDLTPAVNL
                     NFNPSVGIADLNASPVNVSVYPNPATEQITWNVKLQNSTSVNVSLIDINGKVVFNKTV
                     KGKLASYSDTLNVSEFANGVYTLQVKSNDVTNSQKVVIAH"
     gene            21063..21980
                     /locus_tag="PK281_01525"
     CDS             21063..21980
                     /locus_tag="PK281_01525"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY48048.1"
                     /translation="MRFLFILLFPVLFSNSVKAQTATDTIMDFFNMNGSTIVESPNGG
                     FVSGTNGYGDSEKLQAFFPLRSYSVLGAFVWTGKKVYNSGNSNSRICLKVKNFDTSAV
                     STYPYFKGIGATIDSAWFSLSDSNPELSFDAGLKYYSFSSPVLVSSYYALGITFDGLA
                     KDSSGNLLDSVAVFSSAIDSVSVPNMSWEKWNGNYKRISDTWGFDIDLALFPVIDSTL
                     NSASGITNLDFNIYPNPAIDFIQIQANIPANSIVEVIDVFGKTVLKQTVEFNEIGLTL
                     NVHSILSGYYLMKITGNNKIGIRPFLIKK"
     gene            22046..23893
                     /locus_tag="PK281_01530"
     CDS             22046..23893
                     /locus_tag="PK281_01530"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY48049.1"
                     /translation="MKNKLLLLLTCFVGLSLSAQHATRTGNTPVYAKIADTPNTTLPE
                     TPSSASIAITFLDTLYSNDFSDSTVFTQDDASGASSQWVFGTNATIGNGFFSAAFASS
                     TAANGYAMFDYFGFNSDPLFANSSITVGPMDLSTAVAPLTVNFEQYYAKFRDSAQVWF
                     SLDGVNFSLLGDNTDLPLFGVNAQGQTIGAPTANGELKSIFASQLAGQSTVWLRFRYA
                     SDFGGYSWLVDDLTVTTVSVPSIDIELVKTYVTNTTLIDYGITPISQADSVVFGALIK
                     NNGLDVSSYTINFDIQFNGNSVASGSEIVVGTASFDADTLYFNTGYLPDQIGQYTVNL
                     TVDATGDYTPDNNAGSSDFVIDEFVYSPNINLTGGYAESLSGSAAPYAEYSVGQFFYL
                     KNAGTIYAANIAIPKPVGVTNYPLDITIEVREASDLTTLINLTDYVLDNTHPNGLQYK
                     TVLFDPPIDLEAEKFYLLDMSTLDTEKKFNFYAIPGDDDDATRCYGPFGVASAVNWFR
                     GWDFTPCLQMTFNPEIAGLNKTDAGLKISAYPNPANQNVNLVISSDNAVKTSIKLMDL
                     QGKIIMTRNLSFSQIVTESLPVGDLSNGIYILQVATEKGVHTQKITIAH"
     gene            complement(23927..25165)
                     /locus_tag="PK281_01535"
     CDS             complement(23927..25165)
                     /locus_tag="PK281_01535"
                     /inference="COORDINATES: protein motif:HMM:NF014592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HOY48050.1"
                     /translation="MLHPKELSISDFTYHLPDEKIARFPLSERDASRLLIYQNQNIRD
                     GYFNQLPDLFNAGDLLVFNQSRVVQARLHFKTSTGATIEVFCLEPEGQITIPEAMLQQ
                     GRATWFCLVGNAKKWKADQVLTIERLSYQNVRLNASLIARKEDGYIVEFSWEDASLYF
                     AEVLEIVGLLPLPPYLKREVSDRDKGTYQTVYATENGSVAAPTAGLHFSSKVLDSLKE
                     KGVNCQNLTLHVGAGTFKPVKAERMNDHVMHSEEVIATLELIDTLANCEDKVVAVGTT
                     SFRSLESLYWTAVYLKENPNAPLVIEVSQWQPYEKRTSVLPTRKEAFTFLSNALRNAG
                     LKTIQGRTQIIIAPGYSVKVINGLITNFHQPNSTLLLLVSACIGDAWKAVYEHALNTG
                     YRFLSYGDSSYLEIQTKNPG"
     gene            complement(25168..25923)
                     /locus_tag="PK281_01540"
     CDS             complement(25168..25923)
                     /locus_tag="PK281_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006259574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOY48051.1"
                     /translation="MIEVKNICKSFDGRQILDHINVTFEEGKTNLVIGGSGSGKTVLI
                     KCLVALYHIDEGNILYDGRDFSLLDEDQRRDIRKEIGMVFQGGALFDSQTVEENVAFP
                     LQMFGNMSGEERRERVNFCLNRVNLVNANKLFPSQLSGGMKKRVAIARAIALNPRYLF
                     CDEPNSGLDPKTSIIIDNLIHEITTEYNITTVINTHDMNSVLEIGEKIIYLNKGKKWW
                     EGNKQEILETDNQEINDFVYASELFKRIRKASR"
     gene            complement(25920..26660)
                     /locus_tag="PK281_01545"
     CDS             complement(25920..26660)
                     /locus_tag="PK281_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007646497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOY48052.1"
                     /translation="MKIFYFPGHYYLFIRNVFRKPQRFAWFRRQLLYEINSIGINSLP
                     IITIISLFMGAVITIQTAYNIDSPFIPLYAVGLATRDSIILEFSPTIISLILAGKVGS
                     SIASEIGTMRVTEQIDALEIMGINSSSYLVFPKILAAVLINPFLIILSMFLGIAGGWF
                     FGVIAGSVTTTEYLYGIQYVFKPFYVFYALIKTLFFAFIITSISSYFGYYTSGGALEV
                     GSSSTKAVVYSSIFILVANYILTQLLLA"
     gene            26790..>27322
                     /locus_tag="PK281_01550"
     CDS             26790..>27322
                     /locus_tag="PK281_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48053.1"
                     /translation="MNQNDRLELFQTLSNKETCIYYLESNGYDDPYGKFNWILLAGST
                     NHITANADSAKPWEDLKDFLNAPSSAPIIPGFLSYDLKNYLENLKSKNPDLTAFPLLS
                     FLKPECIIGELRNGQSFNYGYSVELEENSIDWEKPIPPIEMQVSMSEESYTQRFDQLK
                     NHILRGDIYEINFCMHFG"
BASE COUNT         8122 a         5560 c         5304 g         8336 t
ORIGIN      
        1 ctcctgaatc atccgtgtgt aattacccgc catactggct gatttattca catctggatc
       61 atttaacgct tcacacgcaa gtgaaatagt tgaaataggt gttttcaact catgcgtcat
      121 attgttgatg aagtcgttct taatgtcgga caactttttc tgacggatga ttgtgcttat
      181 cgtataaaag aacgtagcga tgatgatgag gataaagaag cccgaagaag caaacatcac
      241 ccaaagattg ttaaacacat accgattctg ctctgggaaa taaatcatca gataatcggg
      301 atggtagaat atatcgtgtg gaaaaagtcg aacccgaaag cgcgtcttca tgagctgttc
      361 ggtatagtcg gttgggtgat ccacaaacat tttattgttg atgaagtcat ataaaccaaa
      421 ctcaaatgga gtatttaccc cagctgtttt aagctcaact tttaaaaggg aatcaataag
      481 aattgggtca attcgatctt ccacgccact gttcacattt agatcgaata attcctcgaa
      541 aatatctgtt actaaaccac ttttcttcat caaacgctct aaacgcggat ccttgcgacg
      601 ttcaagcgga ttcaaatcgg acgtaatagc tccaaaagaa gaagcatcac tcgattgacg
      661 agccttcctc agttttttat attcaacttg tcctcccgaa tcggtgcgtg tttcgccaac
      721 aaattcaata cttccgtctt gattcaactg gcggaattcg gtttgtaatt cacccggact
      781 tacttcattc caaaacacca aaccagatgt ggttgtgtct ttacgatcga tgcttttacg
      841 aatgagggaa tcaatcccca gaaagaacat tttcccttgt ttggaagcat caaatcgggt
      901 tcgaacttta ttagccactt cttgtcgctc caccatttcc accacattgt gaagagcttg
      961 gttaacggtc gactcgaaac gttggcgttc caaatcaatg gcattattaa tccagtaaat
     1021 ctgaatgatg atgagaccgc tcagggcgag aatcattaac ccgataatga gccgtatgtt
     1081 acctcggtta atcataggtt gattaatgca acagaaagat cagcaaaatc ttggatgctg
     1141 agttgttcgg cacgcagatc taaaaaagga tgttggaatt ctcttcctgc gataatcggt
     1201 tttagagcat tacgcaaagt ttttcgacgc tgattaaatc ctgttttcac cacattcctt
     1261 aagaaagggt agggaacagg tagttccaca ttttcctttc gggtaagacg aataaccgcc
     1321 gattttactt tcggaggagg cgaaaacacg tgttcgtgaa ccgtaaataa gtactcacac
     1381 tcgaaatacg tttgaagaag tacacttaaa atcccatagg ttttattgcc atgtttcgcg
     1441 gcaactcgtt cggctacttc tttttgaaac atgcccacga tgatttgcac atgctctttc
     1501 tgatcgaggc attgaaacaa aatctgggag gaaatattgt acgggaaatt ccctataaca
     1561 gctcctgtat gatccaaata ttgatctaga ttcatccgaa ggaaatctcc ttcaatgatt
     1621 cttccttcta aagctggaac attcgctttc aggtatttaa ccgattcccg gtcaatttcc
     1681 acgactttta aatcgaagtt tttctccaaa aggaaattcg ttagcacacc cattcccggc
     1741 ccaacttcaa taacctttcg agtagaatcc atggctggaa tgctatcggc aatttgagaa
     1801 gcgattgata aatctttaag gaaatgctgt ccgagtgctt ttttggctgt aacgggtttg
     1861 cgaaatagtg ccatcagacg gtttcgagta aggtgcgaaa agctacaaat cgatctttgt
     1921 atcgggatgc aaaatcggct tgtaaacgag gggcgtgtaa ttccttgtat tcctcgtatt
     1981 tagccatatt gtcaaggaaa tattgaacag agtatgtaaa tccctcacct tcttcggtgt
     2041 ttaatacgcg gaaaaagcgg ttttcaacaa atagtccggt gcttaacact tctggaatat
     2101 gagtggattt catccagttt acccattcga ggtggagttc cggatcgatg ttaacggtaa
     2161 cattataaag gatcatgcag caaagatacg ggaagcaaac cgcttttagg aatcccagtt
     2221 tttgctctta ttttagcaca tacatcagcc cattttaagt tgctgctgca tactatcttt
     2281 gagcatcatc ccaattttac taaaacccgt atgaaacaca ttgtactttt tgcactttcc
     2341 tgtatagcct taaatggata tgctgatcct ggagacactt taggggtatc tctcggttca
     2401 accccatcgt tttctccagc taatcaagta acactcccgc catatcaagg tggttatgca
     2461 tttggggtga attatgatcc gaataacaac tttataggaa ttggacaagg atatatcaac
     2521 gaagaaccag caaatattgt tggtgtgctt agtttgattg gagcaaaatc taaaggagta
     2581 aacaatgttc ccaacactaa attgacgttt ggtttacata atatgtcaaa tagcggtgct
     2641 tttaacatca ttactactac aaatcctccg tcacttgaag caacaaatgg tccctctgtt
     2701 acactcttag gttccaaatc cttattcttc gaagaaatcg atacaacatt aggtgcttat
     2761 aattatgtgg catttgatga tacaattgct gtcaatggca atttggttgt ttcggtcgat
     2821 ttagcggaac taaaaacagc aggtgacata gttggttttt atagtgatgt acccggaaat
     2881 gctttcggaa tcaacttcgc atttcacaag gcaaatgtgg caggagatat catgtggttt
     2941 acttccaact cggttttcca aggtgcccta gatgtgagtt tggcaatttt cccagtgatt
     3001 gctgcagatg ttccggatgc tattttagaa acaagcaaag catttcaagg tgttcgcgct
     3061 ctagcaatgc ccaatccagc taacgatgtt ttgaatattc aacttgaaat aagtgaaacg
     3121 gcgaactata cagttcaact ggttaatgtt caagggcaac ttgtaaaaga gttgtcttta
     3181 ggtgtgaaaa ctcccggggt gcataccgtg gaaacaaccg ttgcggagct tgaatcaggt
     3241 acatactttt actctattat tagtgacaca ggaatacggt ttagtaaaac tatcgtatta
     3301 gcacattaaa atagaatggt ttgtgattga aaacgcgtgt tctttcgggg atgcgcgttt
     3361 ttttattgaa ttacatctcc acgtaatgtc cggaaacgct tacgagcctc aaccacaaat
     3421 aaggaaccgg ggtagtcggt catcaactct ttatataatt ccatcgcctt agcttgattc
     3481 cccaactgtt tctcgtttag ttcagctaat ttgaacaaag cattatcggc caaaatatct
     3541 ttctgatatg tagtaactat tttgtctaat aagctctctg catctgcaaa acgactttga
     3601 cgaatagcga tgttcgcttt gcggtataaa atatcgtccg acaaactact agcaggaaaa
     3661 gcttcttcca aagaatctaa tgtggttagt gccgcagaaa gttttccttg ataaatccac
     3721 aaatcagctc gcgaaaacat gcgtaatcct tcacctgttg tatcaaaaac taaattgtca
     3781 ccaattaata ggctcaattc taacgcgtca ttagcaatta gttttgatgt agcagctttc
     3841 aatacatcta actgcgcttt agcccaatca aattctcctt tataataagc caatcgggca
     3901 tttcggaatt tagcttcttg tccaagtgta tcagtcttca tcgacttttc cacttgagaa
     3961 taagtcaggg tagattccca cacatcaccc gttagcaatt gaatatcggc aacttccaat
     4021 ttaacttcat tcatacttct attcgacaag ccagaattag cctgagtaag cggttccaaa
     4081 atagctaatg cctcttgagg ttgattttta tagaatgctt tcaagtgtgc aagacgaatg
     4141 gctacagcat acgattgttc tcctattcca agttcgcgat aggtttcggt tagcagggat
     4201 tctaattctt gtaattcggc aggagaatag gaagaagaag tgctgagttt tcgatacata
     4261 tcaaccgaaa gctccttttt tgccatcaca tacaagctcg aaattggacc tttttcaatt
     4321 acgtattgaa atgctttttt tccagtctcg aacgcatcgt tactcatgca aatccgagct
     4381 aaattatata cacgttcacc tcgttccttg agtcgtttat cgattgcctt ggcttggatg
     4441 aatgctgatt caaaatcctt tttctgtaca aacatccaaa ctaacaaatc ggagaaaacg
     4501 agattatccg gattacgttg tacttctttc aaaaagcgct ctcgaagcaa ctcggttttt
     4561 tctcccgaaa catccgaagc catcatatct tgtaaactac cctgaatctg ttccaaatac
     4621 tcaccgccgg tagtattgag caaattcatg tattgttcca acatttttcc agtctcgcct
     4681 tgttgcacat acaaagcagc cagctctaaa ttgaaattat atgctcgagg aatccgactt
     4741 gccaattcat acacgcgaat agccattgga atttgtttcc gttgttgaag ggcattcgct
     4801 aagtctgtta cctgacgatc atcgcctggt tctaattctt ttaacgcctt ctcatactgc
     4861 tttttagctt tatcctcttc tccacttact tgatatacat aagctaggtc gattagaaag
     4921 gttaactgac gagggttttt gcgaatttgc ttactcacca gtttttcggc cgatttgatg
     4981 tctttgagat tcaaataaca gctcaccagt tgagaatagt aaaatccggc aggcgcttgt
     5041 tcaaatagtt tctcgtatac atcagcagct ttttgccatt ctccttcggt ataatattgt
     5101 ccagctaatt gctcttctgt agacggcaaa ccttgtccgg gtgcatcatt tccaggttgc
     5161 gctctaagca acacaaatgg tgatagtaga agaataaaaa acaggtaacg caggaaactc
     5221 atggcacgaa tatacaacag gatgttcaac ccctttgacc ttagaaacgc agattaacgc
     5281 atgttgttac atcaaacaaa atcaaacctg atctagagct tgcaataggt cttctaataa
     5341 aagctgagga tcttctaatc caacataaac acgcaccaaa ttccaaggca tgagcggatt
     5401 gtcgtaagaa ggagtgcttg ctaaaccaca cacagggaat acaagcgatt catatcctcc
     5461 ccatgaacag gcaatgagaa aatgcttgag tgaatcacaa aaacgctcta caccagcggc
     5521 cgaatcagta tttatgacca aactgaacat tcctgcgcaa cgtttcattt gcttacgagc
     5581 taattcattc tgtggatgtt gatccgaaaa gggccagatt acacgcgata atttggggtg
     5641 cttttctaaa acaggaagaa taaattcagc tgtatcagcc gaacgattcg cacgaatatc
     5701 caacgtgcgt aatccgcgca ttaacaacca agcgtcgtgt ggcgaaatag caggaccaag
     5761 tgtcatccag tcgctagcaa atagccgctg aatcatttct ctgcttccac aaattgcacc
     5821 tgcaaccaca tccgaatgcc catttaaata cttggtagcc gaatgaacca ccaggtcaat
     5881 tcccatcaca tgtggctgtt gaaatagggg agaagaatag gaattatcga taatgctgat
     5941 gtatccgttt tctttcgcaa aagccgccac agcccgaata tcttgtaact caaaggtcat
     6001 cgagttggga ctttcaataa aaaccatccg tgtattcggt cgcttagctt gttcaaaatg
     6061 cgaaatggaa ctaccgtcga tgaaagtcgt ttcaactcca tagcgcgcta ataaaacatc
     6121 gagtaattta tacgtccagc tatacggttt ctgcacacaa ataacatgat cgcccgactt
     6181 cagatttcca ataacagcaa ccgatacagc cgcacttccc gaaccgcaaa cgagcgcatc
     6241 ttcagcacct tctaatgtag caagtttttg tcgaaggatt cctaccgttg gattatatcc
     6301 acgcgtgtaa aacgggttct cgaattcctt ttgaagcgca tttcgcatcg acgaaacact
     6361 cgggtagcaa aagttggaag attgagcaat agcaggcgac acagcattaa aatagtgttc
     6421 gcgattttct cctaattcat tcagaattac agataatggc agtgattcat tcataaacgc
     6481 gaaggtagct gattagcgaa tcggatggat gaagatcaca actgcgaagg gttgcaaatg
     6541 tgtgaacaat gtgcgcaaaa acatatacac acttcgactg agatttgtgg caatgcagca
     6601 ccgaggtgaa ctattagaat cagcagttag gcagagcgga attccactag ctcgaataac
     6661 taaaaagctc aatcgtagca ggagatggct atataatcaa tttgccaaac ccgatgtttc
     6721 agtagatgtt ttaatggaag ttgggaaatt aattcatcat gattttctac cagaattaca
     6781 cgatttaagt ctcgggaaag cagcgagaaa tataagtatg gacaatactg taacggatta
     6841 tggtgaacaa aatgcacagt attggaaaga taaatacatg cacttgctcg aagagtacaa
     6901 caccttgcta aaaaagcatc aataatcctt ttttaaacga tacacagttc ttggcttcgt
     6961 agctcgccgg agcttgttat ctttgccgcc ccatggcact cgatcagccc caagaagaaa
     7021 ccggtcacga aatgagtttt cttgatcacc tagaagccct ccgttggcat ctggtgcggt
     7081 cggctattgc tattctagca tttgggaccc ttgcatttat ctacaaggat tttatttttg
     7141 acggaatcct gcttgcccct aaataccacg atttccctac ctatcgtttc ttttgctggt
     7201 tatctcaagt gctgtttcac gacgatagca tttgtttggg agacctcaat tttgagctga
     7261 ttagcattag catgtcgggt caatttacgg ctcatataac tgtggcaatt gtaacgggtg
     7321 taattctagc ttttccttat ctactctggg agatttggcg tttcattaaa ccggctcttt
     7381 accaaaacga acgtaaatat gccaacggtt tagttttctg gggaaccttt ctttttttac
     7441 taggtgttgc ttttggatac tatgttatta ccccactaag cgttttcttt tttggaaatt
     7501 atcaggtaag tactttagta agcaatcaaa tcggactaaa cagctttatt tctacggttg
     7561 taaccaccac ttttggaagc gggctcgttt tcgaacttcc aattgtcata tatttcctag
     7621 caaagattgg cttggttggt cctgaattct tgagaaacac acgcaaagtt tcttatgtga
     7681 ttattttgat tgttgctgct gttatcacac ctcctgatgt aacaagtcag attattgttt
     7741 ccattccgct attcatcctt tacgaaatca gtattgtgat tgctagtcgt gtcgaaaaaa
     7801 accgattggc agagttgaag tctagataaa aactgtcgat gcaggactag aaaaacctac
     7861 ctttgtcact tattcaactg ccatgatgtt atctgttttg ttaactcttc tcagcccagg
     7921 gcatatcata ctaattgttg ctgctgtact tcttttattt ggaggaaaaa aaattcctga
     7981 actgatgaaa ggaattgggc aaggcattcg agaatttaag gatgccaaag aaggtaagaa
     8041 cgacgagaat ttaccggaga agaaaaactg attgtcacga ttattatagc ctatgtacac
     8101 atctctggcc gaaacccagg ctgctattgc tgccggaaca acgaccatcg aggcggttac
     8161 tcgccattat cttaaaacca ttgctgaaaa aacggatttg aatgcctttt tggaggtgtt
     8221 tgatgaagat gctttgaaac aagctgctgc agccgatcaa aagtggaaat caggaaatcc
     8281 cggaaaattg gctgggatgg ttattggatt gaaggataat ctgtgttaca aaggtcattc
     8341 agttactgct tcaagcaaaa ttcttgaagg atttgaatcg ctttacaccg ctactgttgt
     8401 tcagcgtttg attgacgaag atgctgttat cattggccga ctcaattgtg acgaatttgc
     8461 catgggcagt tccaacgaga attctgcttt tggtccagta aaaaatccat ttgacccgaa
     8521 tcgtgttccg ggtggatctt caggaggttc tgctgctgct gtagctgcag gtttgtgctt
     8581 agctacgctt gggtcagata ctggaggttc cattcgccaa ccagcttctt tttgtggtgt
     8641 tgtaggtttg aaaccaacct acggacaagt ttcccgatgg ggatgtattg cttatgcatc
     8701 ttcattcgat caagttggcc cattggctaa caatgtagaa gattgtgcta ttattttgga
     8761 agtaattgcc ggtccggatg aattcgattc gaccatgctt caaaaaccag caccagaatt
     8821 ttcaaaacac ttacaggctg aagggaagtt tcgtattgcc tacatgcccg actacatcaa
     8881 taacgaaggg ctagatccgg aaattcgtca atacatgaat tccatgattg aacaacttcg
     8941 ggctgatgga cataccgtaa atgctgttga attcccatat ctcgattatg tgattcctgc
     9001 atattatgtg ttaacgactg ctgaggcttc ttctaatctt tctcgttacg acggtattca
     9061 ctatggatat cgaagtaaaa atgcgcacga tttagaaagc acttataagt tatctcgttc
     9121 cgaaggtttt ggaaaagaag taaaacgtcg gattatgctt ggaactttcg ttttgagctc
     9181 gggatattac gatgcgtatt attccaaagc tcaaaaagtc cgtcgcattt tgcgaaatcg
     9241 taccaatgag atttttgctg aaaacgattt tattctttta cctagtactc ccggaacggc
     9301 tttcccatta ggcgacagaa gtaacgatcc aattaaaatg tacttggaag atatctatac
     9361 cgttcaggca aatttgtgtg gaattccagc tatatctctt cctttaggca aacacagtaa
     9421 cggattacca tttggtgttc agctacttgc gccttcattt aacgaccaac aacttcttgc
     9481 cttctctgca agattgatgt ctgataaggc agaacctgtt cgataatttc ttcgtttatt
     9541 gtcaacaaac ttgtgttgaa tttacatctc aacaccccca ttcttttaga aatttcagtt
     9601 aggtttggtg cttaccacta tctgtttttt tatttcaaca gattgctcag gtactaagtt
     9661 gaagttgaat tcgtttttga tctacgcatg cgtttaaaag ttctcttttc cggattattc
     9721 ctgctgttgg caagccttgc tttcccgcaa aagaagactt ttgtctctgc gcgcgataca
     9781 accgttcgcc ttctggccga tgatcctatt gctgctcaat tggatagtct ggcatacctc
     9841 aaactattcg aaaccactcg atacggtgga gaatttggtc gccctggaaa ttttggtttt
     9901 acgcccgatt ctgttcctgt gtattcgcca caagttttag ctgaacgaat tgctaaaatg
     9961 gatgcccagt cgcctttcaa gctcatatat aatgatgagg taaaggcgta cattaatatg
    10021 tactcgattc gtaaacgcaa tcaggtttcg cgcatgattg gtttatctga attgtatttc
    10081 cctttatttg aagaaacact ggcgaagtac aaaatgcctt tagagctaaa atatttagca
    10141 attgttgaat cagcccttaa tcctgctgct cgctcccgag taggcgcaca aggactttgg
    10201 cagttcatgt atggaacagg taaactgttt aagctgaaca tcaacagcta tgtagatgaa
    10261 cgctgcgacc caataaaatc aaccgaagca gcttgtttgt acatgaaata cttgtattcc
    10321 atttacaaag actggtcatt ggttttagct gcatacaaca gcggtcccgg aaatgtgaat
    10381 aaagctatcc gccgttctgg agggaaaaca aactactggg atcttcgtcc atatttacca
    10441 cgcgaaacag ctggttatgt tcctgcattt attgctgctg tttatgtgat gaattactcg
    10501 aaagagcaca atctgtatcc gattatgccg aaagcaattt tcagcgaaat tgatacgctc
    10561 cacattcatc gccgactcac gtttaaccag ctatcagcag tgttggatat gccggttgaa
    10621 gacatagcct ttctcaatcc ttcctattta ttaggagtga ttcctgaaac tgaaggccat
    10681 catattctca cccttcctaa aagcaaggtg gctaccttta tcaataacga agaaaccatc
    10741 tactcatata aatctccacg cgaaagccgc atggacagct taatgagtat caaacgtgct
    10801 ttaccaattg catctaaaac acacgtggtt cgttcgggag aaagtatctc tgctgtagct
    10861 cgtaaatatg gtgttacggt taacgaaatt aagaagtgga acaaacttaa aagcgctaag
    10921 ctaaaacgtg gacaacgact caaagttagt cctccgtata cggctcaagt cgagcaatcg
    10981 gttagttcaa gcggaggaaa tacagaaata aaaccagctg aagagtcaaa gtctaacacg
    11041 caggcaaccg aagaaaaaac tgaacaggca tccgaatcgg ccacgccaac cgaaacaagt
    11101 aagactacaa cgaaggaaaa gaaaactcct ggaatttcaa cttataaggt tcgtccaggt
    11161 gatacgctat ggaaagtagc tgttagtcac ggaatgacct tggcagagat tcgtaaatta
    11221 aatggcttta cttctaaaac tgttttacga acaggcatga agatcaaagt gaaaacgaaa
    11281 tagacaattt gttactcaca ttaggccgca gaagaacatt ctcttttgcg gcttttttat
    11341 gctaggatgt ttgtatttcc ttgcaaaata catgtatgta agcgaaaaga aagcatgtcc
    11401 gacagaacca tcacaaccga tgtttggatt attctgttgc atctttttgt gtcatgaata
    11461 aaacggaaca ggatttatga cacaactagc tattgtggtt tagtgattaa cccaacgcaa
    11521 aaagcctctg tacgtttcca tacagaggct ttgttataat ttaagcagat tactcagcta
    11581 cgaaaggaac aatcgacacg aacgatttat cgtcgccgcg acgtttgaat tctaccgtgc
    11641 cgtctacaag agcgaacaag gtgtgatctt ttccaatacc tacgttaagg cctggatgat
    11701 gcttggttcc gcgttggcgg atgatgatgt ttccagcaat agccagctga ccaccaaaaa
    11761 ttttcacacc aagacgtttg ctatgtgatt cgcgaccgtt tttggagcta cccgcccctt
    11821 tcttatgtgc catgttttct tgttgggatt aggcgttgat tcctgaaatc tgaattttgg
    11881 tgaacgattg acggtgaccg tttgactttt gatagccttt acgacgtttc tttttgaaaa
    11941 cgatcacttt atcaccctta aggtgtgtga gtactttggc actcacgctt gcaccgctta
    12001 cggtgggagc tccgaccttc actgatccgt tattgtcgat caacaaaacg ttgtcgaact
    12061 taacagagtc accttcattg ttgtcaagac ggtgaacaaa cagctcttgg tctttctcca
    12121 ctttaaattg ctgacctgcg atatctacaa ttgcgtacat agtaagaaag atttgttatc
    12181 ctcttcttcg aaaagggacg caaaagtaat caatcatcac gaatgtgcaa atggttttta
    12241 acaaatctct ccaagaaatg ttaagaaaat tttagaacga tgctccgata catgcctgaa
    12301 catgttcttg aataccaatt ttactccaga tatctttggt ttaaccaatt gataaagaga
    12361 tgaaaaatat cctcatgacc ttctttcttg cgctctgcgg aaccggtctt tttgcacaag
    12421 gaaacagtgg tacactgcaa ggtaaaatta ccgacgatgc cactggtaaa ggaatcgaga
    12481 tggctgttgt aaaactacaa gccggtacgc aaacatttgg tggttacacc gatgtaaatg
    12541 gagattatgc gattaaagga attcctgtag gtaaatacga agttgaaatc agctttgtag
    12601 gatatcaagg attgaaactt tcggaagtac tcataaaaaa ctcaagtatt actttcctta
    12661 ataaaacgct cgccctgaaa gatggaaatg gatacgaatt catttttgaa aaatggaagg
    12721 atgatttgct aggtgcagga acacctggaa ctatgaaacg cctcgggcca gaagacatat
    12781 cattctctgc aaactcgcgt ggtgtaggag atattatcgc gacaagtgtt cctaaagtat
    12841 atatgaaaga tagtggtgga gatttaaatt tctcaggctc tcgctctggt tcaactttat
    12901 atatgattga tggtgttaaa gttattggtg aaccacaact acccaaccaa ggcattgccg
    12961 aaatggttgt tattacaggt ggtttacctg ctcagtttgg tgataccacc agtggtgtgg
    13021 ttttaatcac aacgaagtct tttcattaag ttctgtttag gttgtttttg gtgtgtgacc
    13081 cttgtagcat attgctgcag gggttttttg tttaaacaaa acagcacctt taattgatat
    13141 atttgacgcc cattcttcaa ttatgaatcg cgacagatct gctgctttat ttgaaactgc
    13201 taaaacttat tttccgggtg gagtgaattc tcctgttcgg gcttttaaat cggttggagg
    13261 aactcctgta tttattaaaa aaggcgatgg ttgccggtta tgggatgaag atggaaatga
    13321 attcatcgat ttctgcttta gttggggacc tatgattctt ggtcacaaca acagtaaaat
    13381 ccgtgaaaaa gtgtgggacg caatccaaaa tggaacatca ttcggagctc ctacgcaact
    13441 tgaaaatgaa ctagctcacc tgattctttc caataatcgt tttattgaaa agttgaggtt
    13501 cgttagctca ggtacagaag cggttatgtc ggccattcgt ttggctagag gtgttacagg
    13561 acgcgataaa attctaaaat tcgaaggctg ttaccatgga catgttgatt cacttctcgt
    13621 aaaagccggt tcaggtctca tcacctttgg aaactcatca tctgcaggcg ttcctaaagg
    13681 attggtgaat gatacaatcg ttgtcccttt aaaccgaatc gacctagttg aaaaagcatt
    13741 tgaagaatat gctgatcaac ttgctgctgt tatcattgag ccaattcctg ctaataatgg
    13801 tttgcttcaa gtgagtgccg attttctcag agcacttcgt accatcactc aaaagaacaa
    13861 agcacttctc atattcgatg aagtaatatc tggtttccgt gttggattcg aaggcgcagc
    13921 aggcctatac gatatctctc ccgatatcat aacttacggg aaaattattg gtggtggatt
    13981 ccctgttggg gcttatggtg ccagcaaaga aattatgggt cgtgtttctc ccgatgggga
    14041 tgtgtatcaa gctggaacat tatctggaaa tcccgttgcc atggctggtg gaattgcagc
    14101 tcttaccgaa tgcttaaaac caggcttcta cgaacagctt caattaaaaa cagagcgctt
    14161 tacagccaaa attcttcaac acacgcaaga acgcaattac ccatttagaa tatttcaaac
    14221 cggctccatt ttctggttat cgtttgctag tgaagatatt cacgctgccg aagaaatcga
    14281 cccgaacggc atgagtaaat tcaaagtttt gcatgctttc ctattagaaa acggtgtgta
    14341 tttcggtcct tcaggatatg aagttggttt tatttcgtct gctcataatg aacaagactt
    14401 ggaatatgcg gcacaaaaga tttgtgaagg cctcgattta gtttattcct aaaacaaact
    14461 accgagcaga agtatccaaa agaattggtc tagcatagca ccatctagcc atccggcata
    14521 aaaccattca tcaagccctt catcagcttg agaaatgaga tatccggttg atgcacagtt
    14581 tatcaatagc gcaacggaaa gactaatagt gtagaagccg agttgaaagg aaacaaatcc
    14641 cagaacggtc gtcaatacca ataaacttct gctaataaat ttcagcttgg gtattcccca
    14701 gagcgaggca aacgttttaa cctgattttt ccgatcgtgc tctaaatccc gaatatcaaa
    14761 agggagcgta atggcaaaaa tgaaaatgaa acgttgaatg gttgttaacc aaacagatgt
    14821 gtcgggaagc caagtgtttg actgtgtcat gaagggcaaa aaaaccgtca caaatgccca
    14881 aacagccgaa actaaaaata ttttgatgta gggtaaatcg cgcagtcgaa accaatgtcc
    14941 gttttccttc caaacaggga tgacataaaa aactgcgagt aaaataaacg gactaaatcg
    15001 ggcaagaaac gaaagtgtga ggaaaggata aatcccgcca attcccataa gtaatccaac
    15061 taaggcaaaa acttggagtt ttcgatgatt gtcaattatc cattgatgac gatccgaaac
    15121 ttgaagagca taaataactt cttttcgaaa aagccggtga taattgtaga agaacaaagt
    15181 cgcgccgaaa atgctgataa ataaaagggg tgaaaaaggt agattatgca atcgagttgt
    15241 gcagccatac atggatgcag ctccaaaagc aacccagatg tttccaaaaa ccaccaatcg
    15301 tatccattcc gataaccagg cggaaggttt catgttaatt caaaaccaaa gttaatgtaa
    15361 cgactcccaa aatatatcca gcggcagatc cggcagcact tcgtttggta accatacgtt
    15421 tagctgttgt accaaaacct tctttataat atggattgtc gatatatgct tggttatagc
    15481 cgttttcagc agtaaactta gggagcgtat aacctttcca agcagtataa ccgatcacaa
    15541 acagagggcc gtaaaacata cccgcagcag caccagcaaa accaaacatc atcccgccag
    15601 agccagtttt tctcgcttgt tttaaatagc cattacgtgc atctacttgt ccatttatat
    15661 aaaaggccat ttgttctgct gtgtaccaat tattttctaa tgtgtcttgc ttgtaataaa
    15721 tctgatcagg ttttccttta cttctatgag aatatacctt gtcttgttcg attttcttct
    15781 cccgcttacg gcctgctttt acataataaa cataatcatt ctcaactcgt gaagaactaa
    15841 tcaaaatagg tttgcgatca attagcacaa gtgtatcttg tccaaataga tttgagctag
    15901 atagaattaa gcagaaatat aaaaaccaga atgacttcca agtgggcatc ataattctaa
    15961 aattgcatca aacctaattc attttaggtg tcaacccaaa caaacatcat tttcatatgt
    16021 tttcaaaatg aactgcctta aatgtttgta ttttcgcacg ccattccggc aaattattta
    16081 caaacgctaa cggacaatcc ttctccatga tccgcgattt cgaatcccgt cacatcggcc
    16141 caaatgaagc cgaaacagca aaaatgcttc aggtaatagg tgttaacagc ctggacgaat
    16201 taattgataa aacagtacct gcttctattc gccgaacgga taagatgcag attgctccag
    16261 ctttatcaga attcgaatac ttgcaacata ttcgtcaaat tgctgcgaag aacaaggttt
    16321 tcaaaaccta tatcggattg ggatattaca atgtcatcac cccatcagta attcaaagaa
    16381 acattttcga aaatccaggt tggtacacgg cttatactcc ttaccaagct gaaattgcac
    16441 aaggtcgatt agaagcattg ttaaattatc agacaatggt catggacctt accggaatgg
    16501 aattggcaaa tgcttcttta ttggacgaag caacagctgc tgctgaggcc atgcacatgt
    16561 tctatgctat gcgtaatcgc gatcaagtga aacgcaatgc aaatgtgcta ttcgtatcgg
    16621 ataaatgctt tcctcaaacg atcgaactcc ttcaaacccg ttctgcccca attggaattg
    16681 aacttcaaat tggtaatgcg agtgaattag tttggaacga tcaaatattt ggtgcagtaa
    16741 ttcaatatcc cgatgtggat ggagaaatta acgactatcg cgagttcaca gccgcagcga
    16801 aaagcaatca ttcatttgtt tgtgtatgtg ccgatttaat gagtctagtt ttacttacac
    16861 ctcctggtga atggggcgct gattgtgttg taggttctac acaacgtttt ggtgttccaa
    16921 tgggttatgg tggtccacac gcagcctact ttgctacaca cgaagaattt aaacgcagta
    16981 ttccaggacg aatcatcggt gtttcgatcg attctcatgg gaatccggct cttcgtatgg
    17041 ctttacaaac ccgcgagcag cacatcaaac gcgataaagc aacttccaac atctgtacag
    17101 ctcaagcatt attagctatc atggcttcta tgtatgctgt ttatcacggt ccagatggat
    17161 tgaaacgcat tgccggaaaa atccactctg caacgcgtca tttagcaaca cgattaagca
    17221 gccttggata cacggttaaa acccaagcct atttcgatac cattcgtatt ggattgaaca
    17281 acttatgttc tcaggaattg ttgaatcaac gtgctattca acgtgaaatc aacctgcgtt
    17341 acgaaagcaa tggagatgtt tctttaacga tcgatgaaac aacttctcaa gtcgacatta
    17401 aagccttgtt ggatttattt gcaggtgctg ccgggaaaga tgctggatca attgattcct
    17461 attctactat tccattctgg gaaaacttct cacgcagttc cgcaatttta aaggaacgtg
    17521 tttttaatag ctatcattcc gaaacggaga tgatgcgcta catcaaaaag cttgagaata
    17581 aagatcttgc tttaaatcac tcgatgattt ccttaggatc ctgcaccatg aaactcaatg
    17641 cagcatccga gttaatgcct cttagcttac ccgaatttgc taatcttcac ccatttattc
    17701 catctgatca agcagaagga tacttggaaa ttttccgtga attaagcagc gacctttgtc
    17761 aatctactgg ttttgcagcc gtttcacttc agcctaattc tggcgcccaa ggagaatacg
    17821 caggcttaat gactattcgt gcttatcact taagtcgggg tgatggacat cggaatgttg
    17881 ccttaattcc ttcatcagca catggcacaa atccagcttc agcagttatg gctggaatgg
    17941 aggtagttgt tgttaagtgt gatgaaaatg ggaatgtaga catttcagat ttgaaagcta
    18001 aagcagcaca atatcgcgat caactttcgg ctctcatggt tacttatcct tcaacacatg
    18061 gtgtgttcga agaaggtatc cgcgaaatca cctctattat tcatgagcac ggcggtttag
    18121 tttacatgga tggtgcaaac atgaacgctc aagttggatt aacaagtccg ggtactattg
    18181 gtgctgatgt ttgtcacttg aatctgcata aaacctttgc tattcctcat ggcggcggtg
    18241 gacctggaat ggggccaatt ggagtgaatt cgaaattagc accatttctt cccggtcacg
    18301 gattagtaaa aacaggcgga gatcaagcaa taacagctgt ttcagcttcg ccatttggta
    18361 gctcgttgat tttattggtg tcgtatgcgt acatcaaaat gttaggtcct gatgggctaa
    18421 caaactcaac tcgttacgcg atcctcaatg ccaattacct caaagcaaaa ctggagaaat
    18481 attacccagt gctttataag ggaagtcatg gttttgttgc tcatgaaatg attttggatt
    18541 gccgtgagtt taaacgtact ggtcatgttg aagtaggaga tattgccaaa cgtctgatgg
    18601 attacgggta tcatgctcca acgatttcgt ttccagtacc gggtacaatc atgattgagc
    18661 caaccgaaag cgagggaatt gctgaattag atcagtttgc agaagcaatg atttccattc
    18721 gtaatgaagt agcagaaatc gaagcaggca aagcagatcc agagaacaat gtgattgtaa
    18781 atgctccaca tacggcagtt tctgtcatta gcgactcttg gaataagcca tatagtcgag
    18841 aaaaagcggc gtatccattg agttgggttg ccgataataa gttctggcca agtgtaggaa
    18901 aggttgataa tgcatacggc gacagaaatt tggtatgtag ttgtgcgcca atagaatcct
    18961 atgcccaacc agcttaattg atagaatgat ttaactgaaa ttcaggttaa aacccgtttg
    19021 ttgtaacagc gacaaacggg tttttttgta ttgtgaaagt tattggtatt tgaacataat
    19081 aattctattt ttgcccattg ctatcttccg aatgttctat aacggaacat ggaaataaat
    19141 tggattcaca atcaacacat aaacaaccac acatgaactc taaactttta cttgccatgt
    19201 tcttgggtgc gtctgcaact ctgagcgctc aaaacgccag tacacagtca gtgccagcga
    19261 acacaacaaa aaagaacaat tatgttcacc agcatgcagc tcctgcaagc aattcatttt
    19321 ctaatcgtgt agacatttgg acgaatgact tttcaaatgc tgcagattgg gttcttacta
    19381 acgaagtagg aaataacagt aactgggtaa ttggaacagc agttcctgct ggaaactttc
    19441 ctattacagc aatcgcttct acatcagcag caaatggata tgcacttttc gattcagatt
    19501 tggattgctc tggtaatcaa gtgccaaatt tgactactgc aaactctatc gactgttcac
    19561 ttttccctgc agttgttctt gaatttgaac aacagtatcg tcgttttgat gattctactt
    19621 tcgtattcgt ttctaacgat ggcggaacaa actgggtaaa atatacagtt aatggaacat
    19681 tgacaaacaa tgatgcaaca gctacaaacc cggatgttgc taaagtaaac atcactgcaa
    19741 cagcaggtgg tcaagcaaac gttaaaatcc gtttccaatt ctggagtcca gctacttata
    19801 ctactggtat tggtggagca ccaggatgtg cttatgcttg gatgattgat gatgttaaat
    19861 tagtaactcc tccagattat gaattattgg tatctactgt ttatcatgga gatccataca
    19921 ataatttcga atacggtact tacccacttt ctcaagttga tacagttcgt gttgttgcta
    19981 tcgttagtaa cgaaggaggt caaactcaac aagctacagt taactacaca gtaactcgtg
    20041 gtgctacaac tgtaaataat ggttcttctg caactttcga tatcactcca tttacaagcg
    20101 atactattat tgtaagtact ggttatgttc cagatgcagt tggtaactac tctgtagcag
    20161 caactattac ttctgctaac gaagatttcg atccttcaaa caactcaggt tcttctgatt
    20221 tcgtagtatc tgattatatc tattcaggtc taactggtct tgcaggaaca agtgcactta
    20281 cattctctgg tgctggacca actttcgatg ctagcaaaat tgctcatcag tttattatga
    20341 ttaacaatgc tagtttgaaa gcaatcgata tcgcggtttc ttcattatct gatgatgtag
    20401 atttgattat tgaacttttc gatgctgctg atttaactgt tgctcttggt gttggtgatt
    20461 ttagcatcac ttcttctcat ccaactactg ctcaatacta ttcaattatt attgatgagc
    20521 aatctctaac tgcaggtaat gtttacgttg catcagttgg ttctgaagct actgataaga
    20581 aatttgtatt ctacggtgtt gatggagatc cagatgacgc tacgctagta aatgttccag
    20641 atgataacgg tgttttaact tggttccgta ctgatttaac tccagctgtt aacttgaact
    20701 tcaacccatc tgttggtatt gctgatttga acgcttctcc agttaatgta agtgtttatc
    20761 caaacccagc tactgagcaa attacttgga atgttaaact tcagaattca acttctgtaa
    20821 atgtgagctt aatcgacatc aacggtaaag ttgtattcaa caaaacagta aaaggtaaac
    20881 ttgcttctta ctctgataca cttaacgttt ctgaatttgc taatggtgtt tacacacttc
    20941 aagtaaaatc taacgatgta actaactctc agaaagtagt gattgctcac taatctttct
    21001 tagctcaaaa taaaacccct taagtttgcg cttaaggggt tttttaattt atccttctac
    21061 tcatgcgttt tctgtttatt cttctttttc ctgttctgtt tagtaattcc gtaaaagctc
    21121 aaacagctac cgataccatc atggattttt ttaacatgaa tggttcaacc atagttgaga
    21181 gccctaatgg tggatttgtt tcgggaacaa atggatatgg agattcagaa aaactacaag
    21241 ctttctttcc gcttagatca tatagcgtat taggtgcatt tgtctggact ggcaaaaaag
    21301 tatataattc aggaaattcc aattcacgga tatgtttgaa agttaaaaat ttcgacacat
    21361 ctgccgttag tacttatccc tattttaaag gaattggagc aacaattgat tctgcttggt
    21421 tttcactttc cgattcgaat cctgagcttt catttgatgc tggtttaaaa tattactcat
    21481 tctcatcgcc tgttttagtg agtagttact atgctctagg aattactttc gatggcttag
    21541 ctaaagactc atctggaaat ttattagata gtgtggctgt ctttagctct gcaattgaca
    21601 gtgtttctgt tcccaatatg agttgggaaa aatggaatgg taattataag cgcatatccg
    21661 acacttgggg tttcgatatt gacctcgctc tattccctgt tatagattct acattaaatt
    21721 cagcttctgg aataactaat ttggacttca atatttaccc aaatccagca attgatttta
    21781 ttcaaatcca agcgaatatt cctgcaaatt ctattgttga agttatagat gtttttggca
    21841 aaactgttct aaaacagact gttgaattca acgaaatagg tctaacgtta aatgtccatt
    21901 ctattctttc cggatattat ttaatgaaga taacggggaa taataaaatt ggaataagac
    21961 catttttgat taaaaagtaa aaacaagaag gaatccttct tctaaccatt ttcttttcct
    22021 tatttttgat ttaaaaccca gaaacatgaa gaacaaatta ctccttttac tcacgtgttt
    22081 tgtaggtttg tcgctctccg ctcagcatgc aacacgtact ggaaacactc cagtttatgc
    22141 taagattgcc gacactccca acacgacact tcctgaaacc ccttcaagtg catctatagc
    22201 tattacattc ttggatactt tgtattccaa tgattttagc gatagcactg tcttcactca
    22261 agatgatgct tcaggagcat ctagtcagtg ggtatttgga acaaatgcta caataggaaa
    22321 tggatttttc tctgcagcat tcgcttcatc tactgcagca aatggatatg ctatgtttga
    22381 ctattttgga tttaattcag atccattatt tgccaattca agcattactg ttggaccaat
    22441 ggatctttca actgctgttg ctccattaac cgttaatttt gaacagtatt acgctaaatt
    22501 ccgcgactct gctcaggttt ggttttctct tgatggagta aatttctctt tgttaggtga
    22561 taatactgac cttcctcttt ttggagttaa tgcacaaggt caaacaattg gagctcctac
    22621 tgcaaacgga gaacttaaat caatttttgc ttcacaactt gccggtcaat ctactgtttg
    22681 gcttcgtttc cgttatgctt ctgattttgg tggatattct tggttggtag atgatttaac
    22741 tgtaacaaca gttagtgttc cttctataga tatcgaatta gttaaaacat acgtaactaa
    22801 tacaaccctt atcgattacg gcattacacc aatctcacaa gctgattctg ttgtatttgg
    22861 tgcattgata aagaataatg gattggatgt atcttcttat acaatcaatt tcgatattca
    22921 atttaatgga aacagcgttg caagtggaag cgagattgtt gttggaacag cttcttttga
    22981 tgccgatacc ttgtatttca acaccggtta tttacctgat caaattggtc aatacacagt
    23041 aaatttaact gtagatgcta ctggtgatta cactcctgat aacaatgcag gttctagtga
    23101 ttttgtgatt gatgaatttg tttattctcc aaacattaac cttactggtg gatatgctga
    23161 atcattaagt ggttctgctg ctccttacgc tgaatattca gttggacagt ttttctactt
    23221 gaaaaatgct gggactatct acgcagccaa catcgctatt cctaagcctg ttggagtaac
    23281 aaactatccc ttagatatta ctattgaagt tcgtgaagct tctgatttga caacgttgat
    23341 taatctaacc gattacgttc ttgacaacac tcacccgaat ggattgcaat acaaaacagt
    23401 ccttttcgat cctccaatcg atcttgaagc tgagaagttt tatttgttag atatgtcaac
    23461 tcttgacaca gaaaagaaat ttaatttcta tgctatccca ggtgatgatg atgatgcaac
    23521 tcgttgttat ggtccttttg gtgttgcttc tgctgttaac tggttccgtg gatgggattt
    23581 tactccatgt cttcaaatga catttaaccc agaaattgct ggtttaaata aaacagatgc
    23641 tggcttgaaa atctctgctt atccaaaccc agctaatcaa aatgttaatt tggttatctc
    23701 ttcagacaat gctgttaaaa caagcattaa attgatggat cttcaaggaa aaattattat
    23761 gacacgtaac ttgagtttct ctcaaattgt tactgagtca cttcctgtgg gagatctttc
    23821 taatggtatt tacattcttc aagtagctac cgaaaaagga gttcacactc aaaaaattac
    23881 tattgctcac taatattgag cgattcataa aacaaaaaac cccggttcat ccggggtttt
    23941 ttgtttgtat ttcgagataa gaactatcgc catagcttaa aaaacgatag cccgtattta
    24001 atgcatgctc ataaacagct ttccaagcat cgccaataca agccgatact aataagagta
    24061 aagtactatt gggttgatga aaattggtga ttaaaccatt tattacttta accgaataac
    24121 ccggagcaat aattatctga gttcttccct gaatggtttt taaaccagca tttcttaagg
    24181 cattcgataa aaatgtaaac gcctcttttc tagttggaag tacacttgtt cgtttttcat
    24241 aaggttgcca ctgactaact tctataacaa gaggtgcatt tgggttttcc ttcaaatata
    24301 cagccgtcca atacaagctt tctaaacttc ggaatgatgt agtcccaacg gcaacaacct
    24361 tgtcttcaca attggctaga gtatcaatta attctagtgt agcaattact tcctccgaat
    24421 gcatcacatg atcattcatt cgctctgctt ttacaggctt aaatgttcct gctcccacat
    24481 gaagcgttaa gttttggcag ttaacaccct tttccttcag agaatccaaa acctttgagc
    24541 taaaatgcag tcctgcagtt ggggcggcaa cagaaccgtt ttctgttgca taaacggttt
    24601 gataggttcc tttatctcga tcagaaactt ctcgttttaa atagggcggc aatggtaata
    24661 aacccacgat ttccagaacc tctgcgaaat ataaactcgc atcttcccat gaaaactcga
    24721 cgatataacc atcttctttt ctagctatca aactagcatt taatcgtacg ttttggtaag
    24781 ataaacgctc aatcgttaaa acttgatccg ccttccattt ttttgcatta ccgaccaaac
    24841 aaaaccaagt tgcacgacct tgttgaagca tcgcctcagg aatggttatt tgtccttctg
    24901 gttctaaaca aaacacttcg atagtggcac ctgtgcttgt tttgaagtgc aatcgagctt
    24961 gcacaacacg cgattgatta aataccaata aatcacccgc gttgaacaaa tctggcaatt
    25021 gattgaaata tccatctcgg atattttgat tttgataaat caataaacga gaagcatctc
    25081 tttcagataa agggaaccgg gcaatttttt catccggtaa atgatacgtg aagtcgctta
    25141 tggaaagctc tttcgggtgt agcatactta acgagatgct tttcgaatcc gtttaaacaa
    25201 ctccgaagcg tatacaaagt cgtttatttc ttgattgtcg gtttctaata tttcttgttt
    25261 atttccttcc caccacttct ttcctttatt gaggtaaata atcttttccc caatttctaa
    25321 aaccgaattc atgtcatgtg tattaatcac cgtggtaata ttatactcgg tagtgatttc
    25381 atgaatcagg ttgtcgatta taattgatgt tttaggatct aatccggaat ttggttcgtc
    25441 acaaaataaa tacctcggat ttaaagcaat tgcacgagcg atagctacac gctttttcat
    25501 tcctccactt aattgtgacg ggaataattt attggcattt accaagttta ctcgattcaa
    25561 gcaaaaattt acgcgctctc ttcgctcttc tccactcata ttaccaaaca tctgaagcgg
    25621 aaatgccaca ttttcttcta ctgtttggga atcaaataaa gctcctccct gaaacaccat
    25681 cccaatttct tttcgaatgt ctcttcgctg atcttcatcc aacagggaaa agtcacgacc
    25741 atcgtacaag atgtttcctt catctatgtg atataaagcg actaaacact tgattaaaac
    25801 tgttttgcca gaaccacttc ctccaattac taaattagtt tttccttctt caaacgtaac
    25861 attgatgtgg tcgagaattt gtcgcccgtc aaagctctta cagatattct taacctcaat
    25921 catgctagta ataattgagt gagtatataa ttagccacca gtataaaaat actgctatac
    25981 acaaccgctt ttgtacttga gctgcccact tctaaagctc ctccagaagt gtagtaaccg
    26041 aaatacgaag aaatagaagt aatgataaaa gcaaaaaaca aggttttaat taatgcataa
    26101 aacacataaa atggcttgaa cacatattga attccatata aatattcggt ggtagtaaca
    26161 cttcccgcaa taactccaaa aaaccatccg ccagcaattc ctaggaacat actgagaata
    26221 atcaaaaagg gattaataag tacagcagct aagatctttg gaaaaactaa atacgaggat
    26281 gagtttatcc ccataatctc caaagcatct atttgttcgg taactcgcat ggttcctatt
    26341 tcagatgcga tacttgaccc aacttttcca gcaaggatta gactaataat agtcggagaa
    26401 aattctagga tgatagaatc acgtgtagct aaaccaactg catacaatgg aatgaacgga
    26461 ctatcgatgt tataagccgt ttgaatcgta ataactgcac ccataaacaa cgaaataatt
    26521 gtaattatgg gaagcgagtt tattccaata gaattaatct catacagcaa ttgccttcta
    26581 aaccaagcaa atcgctgtgg ctttcggaaa acattccgaa tgaaaagata gtagtgtccg
    26641 gggaaataaa atatcttcat gtcgctctcc aaacctaagc aaaatacagg atggctttgg
    26701 gtggaataaa ttgattttta aagtctgcct tgttgatagt ttaaatcaga aattaccctt
    26761 cgtaccttgc tcaacgtttt ttaatgtaga tgaaccaaaa cgacaggttg gaattattcc
    26821 aaacgcttag caataaggaa acgtgtattt attacttaga gtcgaatggc tacgatgatc
    26881 cctatggaaa atttaattgg attttattag caggatctac taatcatata acagcaaatg
    26941 ctgattctgc aaagccttgg gaagacttga aagatttcct aaatgctcca tcatctgctc
    27001 caataattcc gggttttctt tcctacgatt taaaaaatta cctcgaaaat ttaaaatcta
    27061 aaaaccccga cttaactgct tttccactcc tttcattctt aaaaccagag tgtattattg
    27121 gtgaattaag gaacggacaa tcttttaatt atggttattc tgttgaatta gaagaaaact
    27181 caatcgattg ggaaaagcca attcctccca ttgaaatgca ggttagcatg tcggaagagt
    27241 cctacacaca acgatttgat caacttaaaa accacatttt acgcggagat atttatgaaa
    27301 taaatttctg catgcatttc gg
//
LOCUS       DAOOQW010000011        27024 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_2123_length_27024_cov_4.838073, whole genome shotgun sequence.
ACCESSION   DAOOQW010000011 DAOOQW010000000
VERSION     DAOOQW010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 27024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27024
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2123_length_27024_cov_4.838073"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1105
                     /locus_tag="PK281_01555"
     CDS             <1..1105
                     /locus_tag="PK281_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007651429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOY48054.1"
                     /translation="ILLDEPTNHIDIESVMWLEDFLVNKANAVLVISHDKAFIDNITN
                     RTIEITMGRMYDYKANYTHYLQLREERRAHQVKAWQEQQKIIADNMAFIERFKGTYSK
                     TNQVSSRERMLEKLDIIEIDEVETAALRLRFPPSPRSGEHPVTVRDVSKSYGDQVIFN
                     QANMSITRGEKVAFVGRNGEGKSTMIKAILGELEVEGQVHLGHNVKVGYFAQNQAALL
                     DPTLTVFETVDQVAEGDIRTQVKNMLGRFMFSGDDINKKVSVLSGGERTRLAMVKLLL
                     EPVNLLILDEPTNHLDLKSKDVLKEALLDFDGTLILVSHDRAFLMGLAQKVFEFKDKR
                     VIEHLETIDEFLERNRIKNIAELNLTNHPSSRS"
     gene            complement(1109..2785)
                     /locus_tag="PK281_01560"
     CDS             complement(1109..2785)
                     /locus_tag="PK281_01560"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOY48055.1"
                     /translation="MNLQASHYRIILFLIGAALFLPFLGAAPLFDWDEINFAESAREM
                     LVTRNYSRVQINFQPFWEKPPLFFWMQVLSMKVFGINEYAARFPNAVFGILTLLTLFE
                     MGRNLRSERFGFLWALCMAGSFLPLVYFKSGIIDPVFNYFIFTSIWFISRAVRHYGTK
                     TATRQSLYAGLCIGLAILTKGPVGLVLACLTFGVFWTFKRFKPVVSFKNLLLFTVSAL
                     FVTLAWFGPETYQHGTWFFNEFITYQIRLFSTPDAGHAQPIYYHFVVVLLGCFPMSAL
                     ALKGLFRRPQFRVDDPDFLLWMKILLWVVLILFSIVTTKIVHYSSMAYLPLSAIAAMS
                     IEEYLRGRQAWKFWQVIGVFFIGFPIGLVMLAIPYVGLNIQQLVPLMKDPFAVQNLQA
                     DVHWSILDLLPGLIWIIGTLVGAYWLWKGTNRTAIVSFFTGTVLATAIFLMTFPKRIA
                     GYTQQAAVEFYQGLQGKDVYVETLHFKSFAQYFYTRKQGFSALETANCKDATGYYHID
                     ALREWYLHGAIDKPVYFVVKCVHANEYLNVPGIEKVGEKNGFVFLLRKPN"
     gene            complement(2782..3438)
                     /locus_tag="PK281_01565"
     CDS             complement(2782..3438)
                     /locus_tag="PK281_01565"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOY48056.1"
                     /translation="MRLMLNRTVSFWIVLGLGLLILLGILLFQDKASGFLAIQLPHSP
                     NLNLVFGILTFPGDGLFAVGIVLLLLIFNTYRNALTLLVAYLSGGILVQLCKLLIFHP
                     TPRPVKWFELNHYPFELPAGLSPHDWHSFPSGHSASAATLFLFIATLTNSKPVHALCG
                     IMALVTGYSRIYLYMHFPEDVFVGLIVGALSVVFTRMWMDNRFRAKHPAWANKNFLKR
                     "
     gene            3463..4380
                     /locus_tag="PK281_01570"
     CDS             3463..4380
                     /locus_tag="PK281_01570"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOY48057.1"
                     /translation="MNAAFICLVFLRFVHVSMSKQGPSVFQISQFAHPAALKPDVYIR
                     TFQEHKQEHPFVMKPHGHDFYLLMFFTKGQGSHTIELETYPVHSGSVFFMSPSEVHEW
                     NLSDDSDGYVVFFNASFYRMEANPQHLSELPFYQSGGKLRYAEVPENARKDLSDVFNL
                     MMHAYRHESTYRERILRAFLDVILFQLSALIAPQQKQLTHAVSIIPALEHLIEKHYAE
                     HQPVTFYAEHLHVSVSQLNTSTRQQLNKTVTELLQERLIAEARRLLAYSNKTVSEIAW
                     QLNFSDNSYFSRFFKKHIGLTPEAFRKNL"
     gene            4418..4855
                     /locus_tag="PK281_01575"
     CDS             4418..4855
                     /locus_tag="PK281_01575"
                     /inference="COORDINATES: protein motif:HMM:NF017935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HOY48058.1"
                     /translation="MFKAIDICITNLNNMSLKDSKLYSIASNKCPHCHEGNFFTFSNP
                     YKLEHFMEMNQRCEVCNEDFRREPGYYFGAMYVSYALTVGLGVLLYLLMCVGFSATVT
                     QYFVSFIVLLIVLIPIFYRTARIIWINFFVRYEKPIQVQKISI"
     gene            4892..5788
                     /locus_tag="PK281_01580"
     CDS             4892..5788
                     /locus_tag="PK281_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48059.1"
                     /translation="MKHRLLILFLCLSFISINAQTYLNETARWNQTYSWSGFDAHTSC
                     TSVYYIAGDSVINDTSYYKVNLQSLCYYTHTVYDSLGNSSQVTDTNATDGFSILIRET
                     NKQFIQRNGETENLLYNFELNDFSAIESATPNPSCGIGSVLLMSHDTVCIGSIGRKRW
                     MVSMSNYPLAYYFIEGVGPSSGFRAPICRNGCPECGYGLTSFVLNGDTLYHGNCTITG
                     IEKTNDLNDFLFQYGMNNLSFQAKNLQWVELYSAAGLRTHILRAEAGKVELNTNQLVS
                     GVYIYRAWIDGEMRSGKVVISQ"
     gene            5923..6123
                     /locus_tag="PK281_01585"
     CDS             5923..6123
                     /locus_tag="PK281_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48060.1"
                     /translation="MNTPSTLEDQLKAMLEARQIRFNQMLKVLDAIPGDTSLSTLTNE
                     QVALINLKLASSGEDECETCSG"
     gene            6183..6256
                     /locus_tag="PK281_01590"
     tRNA            6183..6256
                     /locus_tag="PK281_01590"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6217..6219,aa:Met,seq:cat)
     gene            complement(6353..8680)
                     /locus_tag="PK281_01595"
     CDS             complement(6353..8680)
                     /locus_tag="PK281_01595"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HOY48061.1"
                     /translation="MKKIYLLFLCSFLFFSNAFSQCCTYSINMHDSYGDGWNGATLEI
                     FRNNQSLGIFSASNNGTVDTFQVCTGDSIRLFYTAADYEEENSYALIDAAYNVVYSAG
                     PNPATGIVFNNVGDCNTLPAPGTSACTAILIDTGACVQTSNTNFPGSGLNPGCANYAG
                     ADIWYKLIVPPSGNVYFNTSNGGLNDTGLALWVPTDSSCFTLQAISCDDDGGGGYYSL
                     VAGYDLIPGQTLYIQAFGYGGGTGEFQLCAHDLGIIQFDGSELPIVVINTQNQSIVTE
                     TKIDALMDIKYNGDGNYTLLNQEPNIYSGHVGIEIRGATSAGYPQRPYGFETRDNLGE
                     NLDVELLGMPAENDWVLLSNYNDRSFIRNTLAFKLANWMGQYASRTKLCEVMIDSSYK
                     GIYAFGEKIKRNTNRVDIAKLYSYENAGDDLTGGYIFSQSYWDDNNSFLSNYSPIDHP
                     EMEVHFVYEYPKLDSITLNQRNYLRDYVDSLETALYSSNFADTATGYRKYLDTKSFID
                     YFLINELSRNNDGFKKSEFFFKDKFSNGGKVHAGPVWDFDWAWKNMATCDIFSATDGS
                     GWAHEINDCEPDNPSCGWYIRLMQDTSFQNELRCTYESYRDQFFNTEYLFGYIDSVGT
                     LVQNAQARHFKKWPILGVSGPAPEIGIIPTTYTAELDTLKMWIMKRLAWLEAAIPGHC
                     WPDQTGIMDVENGDLTVYPNPSKGLFELSGWNANNAPVNIQVYDASGRLVLKENCQTK
                     NLQKHSIQLSQPGLYLIQIQLQNRMIRTSKILVEE"
     gene            8984..9484
                     /locus_tag="PK281_01600"
     CDS             8984..9484
                     /locus_tag="PK281_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2384 domain-containing protein"
                     /protein_id="HOY48062.1"
                     /translation="MKKVGNKTQSFNTRRSLKSKAAQTGSVNKSLVYEAGVVYGSSNK
                     MERVNAIRAGIPYDFIEVISKRLNNPIKNVLALIGIPQTTYNKKKSEHLLLDSRNSEL
                     IILMNDLIDFGLEVFNQEEEKFQRWLKKPNLSLGGNSPESMLDTITGINEVKFSLNRI
                     EFGNFA"
     gene            9484..9957
                     /locus_tag="PK281_01605"
     CDS             9484..9957
                     /locus_tag="PK281_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HOY48063.1"
                     /translation="MVVYRIEREKYIDTTLSGVGASLSEGVRWNSLNTRIVYTSESRA
                     LALLEVSVHLDLSEDLPNDRYLIEIEIPDDVLIQEVMLADLPKDWNAQPPSLITQVIG
                     DDFVRYNESAILKVPSTIIPQEFNFLINPNHQDAAKLRVLNAFNMQFDSRLKLKG"
     gene            complement(10355..11410)
                     /locus_tag="PK281_01610"
     CDS             complement(10355..11410)
                     /locus_tag="PK281_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006264440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HOY48064.1"
                     /translation="MNKFLLFPMLAISVGMVACSGSHQPAEQDNAKNLAADKELLTKA
                     QTFFQALPSTAEVLENPMSPEKIALGKLLYFDTRLSATGNNSCNSCHNLSTFGVDNKA
                     FSTGDAGKLGGRNSPTSFNAAIHSMQFWDGRAKTVEEQAGMPILNPVEMAIPNEAALE
                     KKLAAVPMYVEKFKKVFPVDKNPISFKNVALAIGAFERTLLTPSRFDKFLGGETTAMN
                     AEEKAGLSLFMETGCTTCHSGPSLGGTMMMKFGVNTDYRPLTGSTNGDNGLMDLTKKE
                     SDKDIFKVCGLRNVEKTSPYFHDGSVKSLNDAVRIMAKTQNNKDLNPEQVTSIVKFLG
                     ALTHDIPDDVKAKPVEL"
     gene            11719..12480
                     /locus_tag="PK281_01615"
     CDS             11719..12480
                     /locus_tag="PK281_01615"
                     /inference="COORDINATES: protein motif:HMM:NF019013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HOY48065.1"
                     /translation="MFQQSVFTQSFHLNQSRNNHSKFLSFIIGIFFYLGFSIIAQLSA
                     QNLAKFSVVDMPEASTDLSNREVAIAVSRSILQANWEELDKLLDDKFTYTGDGYVFTK
                     DQYIGFMQDMRAAFSNFEMILDKSVMQGDHVSFKFVSHAVNTGKFMGAPANKKHLEVN
                     GIFMRNVKNGKVIQEWQTTDLLGTMKQIGGGALFFYSVFVGGFNVQSAPPVRKPNDFL
                     NINGNANTYDQMTSKQKNKYVKKYLKDWKKATNKK"
     gene            complement(12563..14959)
                     /locus_tag="PK281_01620"
     CDS             complement(12563..14959)
                     /locus_tag="PK281_01620"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HOY48066.1"
                     /translation="MRFILSALLVSLFPTVQAQVASIEHPSGGPSVADAATLDTRSDS
                     IDISHTTINLDFSALPTANLIGACQVNFSARVDNINQITLDLQALTVDSVKQFGEAVT
                     FNHSGTKLRIDLLSPLQTGNNSSVEVFYHGTPVEDASGFGGFSFSGGYAYNIGVGFAA
                     DPHNFGRVWFPCFDNFVERCTFSLIVKTPENLKAYCGGILEVDTVENGFRTRHWTLNQ
                     EVPSYLASVAVAPYTEVNYTYTSMLNPQLNVKLAAVPGDTTAMKNAFVSLEAIFHEFE
                     SHFGPYRWDRIGYVMTPFQAGAMEHASNIGYPRDLLATGAAGNQHIMAHELSHHWFGD
                     LATCRTASEMWLNEGFASYCERLFDEWLISRATYDAQVRANHRTMVNLCHVKDGGYWP
                     LSNVPQAYTYTTSTYERPSDIIHTLRTYMGDSLFYVGLKHYFNTYTFRDANSNDLRDA
                     LEAATNVPLHDFFADWVDTPGWGQFEIDSMIITGGIGNRFYYSQKSAGNTHTYTNVPF
                     ELTCRGSNFELFTAQVVLNGPNGFVDVTVPFEPVYASLNRNEAISHAVTAEERFIKTT
                     GNANWANALLEINTSTVQDSVLIRVEHNWVAPDPFKQAFAMYRLSPNRYWHVDGVLRD
                     GWKASAKFTYNGRTSSTSSGWLDNELITDETKLVLLYRKDNGSDWTVLDATKNTLSST
                     TDKYGSFTLDSLMLGEYTFAEIDSSLGIQPITIEENSSLKVFPNPAKTQVSLTWDKED
                     MRYAELFDMSGRSMGRLMIPSGMRGWVFPVEHLAAGTYLMRLETKRGKFVQSKVIIEK
                     "
     gene            15082..15816
                     /locus_tag="PK281_01625"
     CDS             15082..15816
                     /locus_tag="PK281_01625"
                     /inference="COORDINATES: protein motif:HMM:NF026758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4625 domain-containing protein"
                     /protein_id="HOY48067.1"
                     /translation="MSKRFPLGWMMLVLLAAACKKDPVDKIAPQVNDFSVPADSSSFN
                     GNALTFEATLSDDMELSQCKVIIKPTSGAYITSDTLALPYWNFTRVYDVHSFNDIISE
                     TISIPDTIASAWYNILLSIVDASGNLSQTDTNVVFIRNNADSIPPTLILQWPADLQFI
                     SKTGSLHITSDASDNQELSAIVWRIFEGSTQIHTETKYLNANPESVEFDFSLSGLSNG
                     LYTFELTLYDRMLNSSKEQRNFVVID"
     gene            complement(15819..16328)
                     /gene="ribH"
                     /locus_tag="PK281_01630"
     CDS             complement(15819..16328)
                     /gene="ribH"
                     /locus_tag="PK281_01630"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009745292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HOY48068.1"
                     /translation="MSSAHQNLSSRKMVNITNIDHFRFTVIVAQWNKEVTGELEKGCV
                     ETLLLNGIPQQNIRVIAVPGSWELISAARLAVTHSQTDAVICIGAVIKGDTPHFEYIC
                     QGVTNGLATLCSTQDIPVIFGVLTVNTLQQALDRCGGIHGNKGDEAAITAIELAALSR
                     DIRSKEPLF"
     gene            complement(16328..17026)
                     /locus_tag="PK281_01635"
     CDS             complement(16328..17026)
                     /locus_tag="PK281_01635"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOY48069.1"
                     /translation="MADKTTPKHDTLQDIESSLTNTEMFIERNQKKLLMVLAAIIVVV
                     GGVLAYKQFIVKPKEAEAQNAMFKAEYYFEKDSFNLALNGKGEVEGFLAIMDNYSGTK
                     SSNLAHYYAGVCYLQTGKFQEAIDQFDAYDGADMMTSAMAMGAKGDALMELGNTQEAI
                     DQYVKAAGKNENGFSTPMFLMKAGYAYEDLKNYDKALEIYTKVHKEYFQTSEGREAEK
                     YIARLEGMKENGGK"
     gene            17209..18300
                     /locus_tag="PK281_01640"
     CDS             17209..18300
                     /locus_tag="PK281_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013749965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HOY48070.1"
                     /translation="MQLSELHLVNFRNYPEADLAFGQSIVVFTGNNGSGKTNLLDAIH
                     YLAFCKSFLNPIDSQNIRNEEPFFVVQGSFQNEESKDAVYCGIKRGQKKQFKRNSKEY
                     ERLSEHIGLFPLVVISPSDIALITEGSEERRRFMDSVISQYDKPYLEHLIRYGKILQQ
                     RNAYLRQGNSQADMLEIWDEQLAQYARPVFEARKLFLARIKPLFQQVYSFISNSTETV
                     ELTYESKLFDADPYQLLQQTREKDQILQYTTAGIHKDDLGMLINGFPLRKYASQGQQK
                     SFLIALKLAQFDVLSEVKQMKPLLLLDDIFEKLDQDRITALMTLVSQQHFGQIFITDS
                     HPERIAEILRGIQATFDHFTVHQGTVQSA"
     gene            18297..18590
                     /locus_tag="PK281_01645"
     CDS             18297..18590
                     /locus_tag="PK281_01645"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HOY48071.1"
                     /translation="MKRKSNDSSVTELIDLWLKQYGLNGKYREFRMLQSWSELMGPMI
                     ANRTDDIRIYEGILYIQLSSATLRNELSFAKSKIIQSMNEKAGEDLIKDIVFR"
     gene            complement(18733..20502)
                     /locus_tag="PK281_01650"
     CDS             complement(18733..20502)
                     /locus_tag="PK281_01650"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HOY48072.1"
                     /translation="MRLTKKYCLLVLILLTQVVSSQTYLPPTLAWKGKSERIMRSNKD
                     PLVTPFELSGGMNSATKQELGDWFEKLQKTFPDVKQESIGKSAQNRDIWMYSIRRAGK
                     EKPTFLFQAGIHAGEIDGKDAGMLFFRDVLEIQPQLLQNVNVLFIPILNVDGHERASE
                     FNRINQRGPTVMGWRSNALNQNLNRDYTKLDTKEIQSVLAVINRENPVLYMDIHVTDG
                     ADYQYDITYGFQGEHAYSPAISTWLKTKFRSQVDVALTSNNHIPGPLLFAVNDDDFKD
                     GNNEYTFSPRYSHGYGDARHLPSILVENHSLKPFRQRVLGTYVLLFETLRVLSLEGTS
                     LNTAIASDQKRKPEALPLTWDFPKTASDSMKFAGIASKKVKSEVTGVEYVEWSGKAEN
                     QHIPVRKNNVATSHVARPSYYVIPVQFEEVISRLKQHGLEMQSIQTDSTIQLSMYRLS
                     NPQIQGKLPNQGRCRLKADAKEESMQVVFPKGSMLVSTNQALGDLAVLLLEPEFTDSY
                     FQWGFFPECLERTEYFETYVLEPYAADQLNKNPTLKSAFESKKSSDAVFAKDQQAQLR
                     WFYEQSPYADKTYLIYPVGKIVK"
     gene            20694..27002
                     /locus_tag="PK281_01655"
     CDS             20694..27002
                     /locus_tag="PK281_01655"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOY48073.1"
                     /translation="MLKIAKSLLLLFIFILLQIPVFAQLTLGFNSNGNTAGCAPYTLT
                     FPITNIAGNPANTTYVVNFGDGSPVLNFTQGNIPASVTHTYTTNSCGSAFQTTNNVFG
                     ATITASSPTNSPFTAAVSPIVISSPPDAIISPLTSTVCVGSSVSINNNSSQGVYIDPS
                     AGYTCSTTGAISYWTISPAAGWSASAASMGSNNGFPNASDFDVWSQGATPLSVTFNTA
                     GSYRVKIWIANTCGLDTMSVPVCVVPPPNPQFNLNPSFGCIPPNLSVTANNTTAVTPS
                     SCYTPTYSYNWSISPSSNWAFNGASNASSTSPSFSFLSAGTYTVTLTASISALSGCSA
                     SINHPVTVNQAPTVNAGLDQSVCVGSPAIQLTGSPTGGTWSGTGVNASGLFTPTTTGN
                     FTLTYTIPASAGTGCPAVTDQVIVSVITPTAANAGADQSLCPGTPAIQLNATPTGGTW
                     TGTGVTSGGVFTPTTPGSYTLTYSFGTGPCAVSDQKVITVYSPPTVSVNSPVVCAGFN
                     ATLTAVGAAGLGPYSYAWSPSAGLSSGSGASVTTSPAPLSSTSYTVIVTDAHTCTASA
                     VSSITVNPLPSVAAGSDISVCNNAQVTQLTGTPTGGTWSGTGVTAGGAFTPPGLGNYT
                     LTYSYTNANTCSSSDQIIVSVVNPSPAGAGSDQQICVGATPLQLNGTPAGGTWSGAGV
                     STSGLFTPTTPGNVNLTYSFGSGTCASTDQMQMTVYAAPTVNVNNPTVCEGTPATLTA
                     VGAGGLGPYSYSWNPSAGLSAAVGASVVANLTTTTSITVTLTDAHTCLATDVALVTVN
                     PVPVVNAGADQTICLNPSTIQMNGTPAGGTWTGDNVTTSGAYTPVAAGTDSLFYTIST
                     SGCTRSDTMVVNVQVPGSLQLTPDTTVCLNSGNFQLYAATLGGNWTSSTATLTSSGIF
                     TPDAVGVFVVSYSVGAGFCAVGGSLQVTVIAPPTVDAGSDVTLCANSPAYTFPGESPT
                     SGGTWSWSGTGITNTTTGVFSPSISGAGVFPITYTFTSTATGCSASDGLQVTVNALPN
                     IVLNPAVLNVCLTPFGSQLTASPAGGFWTGAGLTYGSNVIAPQDTATYTPSAAGSFSV
                     YYTYTDANTCVNRDTVLINVSVPTPANAGSDTAFCFSNLAHQLTGTPAGTWINPSWLN
                     ANGTFISDTVGTFNAIYAVGGGSCLVYDTALVTIHPLPVVHAGLDQQLCAADPCFNLA
                     APSPLGGTWSGPGIANAASGQFCAATANNGVNQILYTYQNPTTTCSNTDTLLVTIVPM
                     PVPGVAINPLFCINTPVQVTNLSSGAAATFEWNVKNTVTGALVQTSTLASPVVNIATA
                     GTYQIEQICYSIYNCETRDTAYFTVVAPPIAAFSLADNIVCGPHQETVTNTSSGYQIS
                     FLWDFGPSGPGSTDTIPVLPVFAAPIIADSLYHVTLSVSNMCGTRTAVDSVIIRPVPV
                     ALIGTDYSQGCSPFSAVIQNVSYGSPDWFMWDLGDGTTSTDSLPPAHTYTTTDTVSFI
                     PITLQIGNTCGNANAATNVIVYPDHINPTAQTPIVGCSPFEVDFNFPLGDLTYYLWDF
                     GDSTGIAGDSVSHIYENAGVYHVMLTVSNFCLVDTILNTVTVYEGPDLDFTIDQPSIC
                     QTNQIQISNTSQNSAFVNIDFGDGSTSPISSSVNHVYNSTGTQTIQMYGVNPQTGCVD
                     TVSQTVEVVPFPVINVTADPDTGCMPLLVQFFNSSSLATGYEWHFPDGTSSILAEPQI
                     MLAQSGDFQAQLIAHNYQGIGADCPDTAYVTIHVNPSPQSSFILAADSACGPPAQTAV
                     SNTSTGGNSYTWLWESTSSTEFEPQLTFTDTGFHQVRLVAENLFACRDTSASGFMVLG
                     QPQIALSIDPPNGCMPHEVTFDNLSLYGNQVQWAFGDGQFSTLDSVSHVYEEAGLYSI
                     ELLVSSGGICSEDSILVEVIEVYPKAFAGLEISPQFISESLPQINLTNTSALATAYQL
                     YVEDDLVSGIVPPTYMFENPDTGLVQLMLIANNMFNCPDTAYSDVYIKSSPNIYIPNS
                     FTPNDDGLNDGFRPYMDRTPSEYYFAIYDRWGHVVFETNDRDKYWDGTFKNKGKKAIK
                     QDVYVYKLTAIFSEEEVFNLFGNITVIH"
BASE COUNT         7865 a         5557 c         5476 g         8126 t
ORIGIN      
        1 aattttgttg gatgagccta cgaatcacat cgacattgaa tcggtgatgt ggttggagga
       61 tttcttagtg aataaagcga atgctgtttt ggttatttca cacgataagg catttatcga
      121 taacatcacc aaccgtacaa tcgaaatcac gatggggcgg atgtacgatt acaaagctaa
      181 ttacacgcat tatctgcaac tccgtgaaga acgacgagct catcaggtta aagcttggca
      241 ggaacaacaa aagattatag ccgataacat ggcttttatc gagcgtttca aaggcacgta
      301 ttctaaaacg aatcaggttt cttcgcgtga gcggatgctg gaaaaattag atatcatcga
      361 aatcgacgaa gtggaaactg cggctttacg attgagattc ccaccatcac ctcgctcggg
      421 tgaacatccg gtaactgtgc gcgatgtatc gaaatcgtat ggtgatcagg tgattttcaa
      481 tcaagcgaat atgtccatca cccggggtga aaaggtagct tttgttgggc gaaatgggga
      541 aggtaaatcc accatgatta aagccattct aggcgaattg gaagtggaag gacaagtgca
      601 tttgggacac aatgtgaagg tgggctattt tgctcaaaat caagcggcat tgctcgatcc
      661 aacactaacg gttttcgaaa cggtagatca agttgccgaa ggtgatattc gtactcaagt
      721 caagaatatg cttgggcgtt tcatgtttag tggcgatgat atcaataaga aagtatctgt
      781 tttatcgggt ggagaacgca cacgtttggc aatggtgaaa ctcctgttgg aacctgtgaa
      841 tttgcttatt ctcgatgagc cgaccaatca cctcgatctg aaatcgaaag atgtattgaa
      901 agaagcattg ctcgattttg atggtacatt aattctggtt tctcacgacc gtgccttctt
      961 gatgggatta gctcagaaag tattcgaatt caaggataaa cgggtgattg aacacctcga
     1021 aaccattgat gaattcttgg aacgaaatcg aattaagaac atcgctgaat taaatctaac
     1081 gaatcatcct tcaagtcgtt cctaagcttt aattcggttt acgaagtagg aagacgaatc
     1141 cgtttttttc tcccactttc tctatgcctg gtacgttgag gtattcgttt gcgtgaacgc
     1201 atttgactac gaaatacact ggtttgtcga tagcgccatg caagtaccat tcgcgtagtg
     1261 catcgatgtg ataataaccc gttgcatctt tgcaattggc tgtttctagt gcagaaaagc
     1321 cttgttttcg ggtgtaaaaa tattgggcga aacttttaaa atgcaaggtt tctacataca
     1381 catctttgcc ttgtaatcct tggtaaaact caacggctgc ttgttgtgta taacctgcaa
     1441 tacgtttagg aaacgtcatc agaaaaattg ccgttgccaa gaccgttccg gtgaaaaatg
     1501 agacaatagc tgtgcgattg gttcccttcc aaagccagta agctccaacc aaggtgccga
     1561 taatccaaat caatccgggt agtaaatcca atatcgacca atgaacatca gcttgaagat
     1621 tttgcactgc aaatggatct ttcatcaaag gcactaattg ctgaatgttg agtccgacat
     1681 acggaatcgc caacattacc aaaccaatcg gaaatccaat gaaaaatacg cctatcactt
     1741 gccagaattt ccatgcttgt cggccacgta aatattcctc tatactcatg gctgcaatgg
     1801 cactaagcgg taaataagcc atcgaagaat aatgcacaat cttggtcgta acaatcgaaa
     1861 ataaaatcaa tacaacccaa agcagaattt tcatccacag taaaaaatcg ggatcatcaa
     1921 cccgaaattg aggacgacga aacaatcctt tcaaagccaa agctgacatt ggaaaacaac
     1981 ccaataaaac cactacaaaa tggtaataga taggttgagc gtgtcctgca tcgggagtag
     2041 aaaagaggcg aatttgatag gtgataaatt cgttgaaaaa ccaagtgccg tgttgatagg
     2101 tttcgggacc aaaccaggct aatgtcacaa acaaagcact aaccgtaaaa agcagtaaat
     2161 tcttgaatga aacaaccggt ttaaatcgtt tgaacgtcca gaaaactcca aaagttaagc
     2221 aggcgagaac gagcccaact ggacctttag tgagaatagc taatccaata cacaaacctg
     2281 catagagcga ttgtcgagtg gcggttttag tgccgtaatg acgaacagca cgggaaataa
     2341 accagatgga ggtgaaaatg aaataattga aaacagggtc aatgattccc gatttgaaat
     2401 atacgagcgg taagaaactg ccagccatac aaagtgccca gagaaatccg aaacgttcgc
     2461 tacgcagatt gcgtcccatt tcgaataaag tgagtagggt aagaattcca aatacggcat
     2521 tcgggaagcg cgcagcatat tcgtttatgc caaagacttt catgctaagc acctgcatcc
     2581 aaaagaagag cggtggtttt tcccagaatg gttgaaaatt gatttgaaca cgtgaataat
     2641 tcctggtaac caacatctcc cgcgccgatt ctgcaaagtt gatttcatcc caatcgaaaa
     2701 gaggtgctgc gcccaagaat ggaagaaaca aagccgcccc aatcaggaat aaaataatcc
     2761 gatagtgtga agcttgtaga ttcatctttt gagaaagttt ttatttgccc aagcgggatg
     2821 ttttgctcga aagcgattat ccatccacat acgtgtaaaa acaacggata aagcaccaac
     2881 tatgagtcct acaaagacat cctctggaaa gtgcatgtat agatatatgc gtgaatagcc
     2941 agtaacaagc gccatgattc cacagagcgc atgtacgggt ttgctattcg tgagtgtggc
     3001 aatgaataga aacaaagtcg ctgctgatgc cgaatgtccc gatggaaagc tatgccaatc
     3061 gtgcggactc aaaccagcag gaagttcaaa tggatagtgg tttaactcaa accatttaac
     3121 aggacgagga gtaggatgga agatgagtaa tttgcacagt tgcaccagaa ttcctccgct
     3181 gagataggca accaataaag tcagggcgtt gcgataggta ttgaaaatca ataggagcaa
     3241 aacaattcct acggcaaaca gtccatcacc tggaaacgtt aaaatcccaa atactaaatt
     3301 gaggttaggt gaatggggta attggattgc taagaaacct gatgctttat cctgaaatag
     3361 tagaatcccc aataaaatca acaatcccaa gccgaggaca atccaaaacg aaacagtacg
     3421 gtttagcatt aaacgcatgg cgcaatttag gcaaatgtac ctatgaatgc agcgtttatc
     3481 tgcttggtat tccttaggtt tgtccacgtt agtatgtcga aacaaggtcc atcggtcttt
     3541 caaattagtc aatttgcgca cccagctgct ttaaaaccgg atgtgtacat tcgaacattt
     3601 caggagcaca aacaggagca tccatttgtg atgaagccac atggacatga tttttacctg
     3661 ttgatgtttt tcacgaaagg gcaagggagt catacaattg aattggaaac gtatccagtc
     3721 cattcgggat cggtattttt catgtctcct tccgaagtac atgaatggaa tttaagtgat
     3781 gattcggacg gatatgtggt gtttttcaat gcatcgtttt accgtatgga agcaaatcca
     3841 cagcacttat cggaattacc tttttaccaa tcgggtggga agttgcgcta tgcggaagtg
     3901 cctgaaaatg ctcgtaaaga tttgtcggat gtatttaact taatgatgca tgcatatcga
     3961 catgaaagta catatcggga acgtatttta cgtgcttttt tggatgttat attatttcaa
     4021 ctgtcagctt taattgcacc tcagcagaaa cagttgacgc atgcagtttc gattattcca
     4081 gctttagaac atttgattga aaagcattat gccgagcatc aaccagtcac gttttatgcg
     4141 gaacacttgc atgtttccgt atcacaactg aatacaagta cacgacaaca gctcaataaa
     4201 accgtcaccg aattactcca agaacgctta attgctgagg cgcgtaggtt attggcttac
     4261 tcgaataaaa ccgtgagtga aattgcctgg cagctcaact ttagcgacaa ttcgtacttc
     4321 agtcgattct tcaagaagca tattggttta acgcccgaag catttcgcaa gaacctgtga
     4381 aatgtaccat cattcggtga aattgtacca taactgaatg ttcaaggcta tcgacatttg
     4441 tatcaccaac ctgaacaata tgtcgttaaa agacagcaaa ctttacagca tcgcctcgaa
     4501 taaatgtccg cattgccacg aagggaattt ctttacgttt tctaatcctt ataaactgga
     4561 gcatttcatg gaaatgaacc aacgttgtga ggtgtgtaat gaagattttc ggcgagaacc
     4621 agggtattac ttcggggcga tgtatgtgag ttatgcattg acagtaggat tgggagtgtt
     4681 gttatactta ttgatgtgtg ttgggttttc tgctactgtt acacagtatt ttgtgagttt
     4741 tattgtcttg ttaattgttt tgattcctat tttttatcga acagctcgta taatttggat
     4801 taactttttt gtgcggtacg aaaaacccat tcaggttcag aagattagta tatagtttgg
     4861 ataatttgtt ttagatgagt agtttgctgt tatgaaacac agactactca ttttgttttt
     4921 atgcctgtcg ttcatttcaa taaatgctca aacgtatttg aatgaaacgg cacgttggaa
     4981 tcaaacctat tcttggtcag gatttgatgc acatacttca tgtacttctg tttattacat
     5041 cgcaggagat tcggtaatta atgacacaag ctactataaa gttaatttac aaagcttgtg
     5101 ttattatact catactgttt acgatagttt ggggaatagt tctcaagtaa ccgatactaa
     5161 tgcaacggat gggttttcaa ttctcattcg agaaaccaat aagcagttta ttcaacgcaa
     5221 tggtgaaact gagaatctgt tatacaactt tgaacttaac gacttttctg ccatcgaatc
     5281 agctacaccg aatcctagtt gcggtattgg tagtgtctta ttgatgtcac atgatactgt
     5341 ttgtattgga tcaattggac gcaaacgttg gatggtaagt atgtcgaatt atccattagc
     5401 ctattatttt attgaaggag ttggcccgag ttccggtttt cgagcaccaa tttgtcggaa
     5461 tggttgtcca gaatgcggtt atggcttaac cagttttgtg ttgaatggtg atacacttta
     5521 tcatgggaat tgcacaataa cgggaattga gaaaactaat gatttgaacg acttcttgtt
     5581 tcaatacgga atgaataatc tttcctttca agcgaaaaat ttgcaatggg ttgaattgta
     5641 ttctgctgcc ggattaagga cgcatattct ccgcgcagaa gctgggaaag tcgaattaaa
     5701 tacgaatcaa ttagttagtg gtgtttatat ctatcgtgca tggatagatg gggaaatgcg
     5761 ctcaggaaaa gtggtgattt cgcagtgatt ttctaattga ttgagtgaca aggaatcgag
     5821 gtttgaatca acaaaaagtg aataagtcgg ttggcatcct acccaaaatg agtgttacca
     5881 agtgagcgcg cttctcgatt tttgtggaaa gaccaaacag aaatgaacac gccatccaca
     5941 ttagaagatc aattgaaggc gatgcttgaa gcacgtcaga ttcgttttaa tcagatgtta
     6001 aaggtgcttg atgccattcc gggcgacacc agtttaagca ctttaacgaa tgagcaagta
     6061 gcattaatca acttaaaact cgcgtccagc ggcgaagacg agtgtgaaac ttgttctgga
     6121 tagagaaaat tttgcagggg ctttgcaaaa ttgtcaattc gtgtatattt gcagccccaa
     6181 acggcggggt agctcagatg gttagagcgc aggattcata accctgaggt cacgggttca
     6241 actcccgtcc ccgctactag aaataaaaaa gacgcttcat ggcgtctttt tttttgtttc
     6301 tactgaagca gagaacatat tcgttaatcg ttgacttcag tattttaatt ccctattcct
     6361 ccaccagtat tttactcgta cgaatcattc tattttgaag ttgaatctgg attaaataca
     6421 agccaggttg acttaattga atgctgtgtt tttgtagatt ctttgtttga caattttcct
     6481 tcaaaaccaa tctgcctgaa gcatcgtaaa cctgaatatt aacaggtgca ttgtttgcat
     6541 tccaaccact taattcaaaa agtcctttgc ttggatttgg atatacagtt aaatcaccat
     6601 tttcgacatc cataattcct gtttgatcag gccaacaatg ccccggaatg gcagcttcta
     6661 accaagctag acgcttcatg atccacattt ttaaggtgtc taattcggca gtgtaggttg
     6721 ttggaataat gccaatttct ggtgctgggc cactaactcc taaaataggc cattttttaa
     6781 aatgacgagc ttgggcattt tgtacaagtg tacctacact gtcaatatat ccgaataagt
     6841 actccgtatt aaagaattga tctcggtatg actcatacgt gcagcgcagt tcattttgga
     6901 aagacgtgtc ctgcatcaag cgtatatacc agccacaaga aggattgtcg ggttcacaat
     6961 cattaatttc atgtgcccat ccggaaccat ctgttgctga gaaaatatcg caggttgcca
     7021 tatttttcca agcccaatca aaatcccaaa ccggaccagc atgcactttt cctccattgg
     7081 agaatttatc tttaaaaaag aattcgctct ttttaaatcc gtcgttatta cgcgataatt
     7141 cgttaatcag aaaataatcg ataaatgatt tcgtgtctaa atacttgcga tatcctgtag
     7201 cagtatcggc aaaattggag ctgtataaag cagtttctaa actatccaca taatcgcgta
     7261 aatagtttcg ttgatttaga gtaatagagt cgagtttggg atattcatac acaaaatgaa
     7321 cttccatttc gggatgatca attggcgaat aattcgataa aaaactattg ttgtcgtccc
     7381 aataactttg cgaaaaaatg tatccacctg ttaaatcatc tcctgcattt tcgtagctat
     7441 ataatttagc aatatcaacc cgattcgtat tccgttttat tttctcccca aatgcataaa
     7501 tgcctttgta ggaactgtcg atcatcactt cgcagagttt ggttcgagaa gcatattgtc
     7561 ccatccaatt ggccaattta aacgccaagg tgttacgaat aaacgaacga tcgttgtaat
     7621 tcgataatag cacccaatcg ttttccgcag gcatgcccaa taattctaca tccagatttt
     7681 ctcccagatt gtcgcgcgtt tcgaacccat acgggcgttg cggataacca gctgatgttg
     7741 caccacgaat ctcaattcca acgtgtccgc tataaatatt cggctcttga ttaagcagcg
     7801 tgtaattccc gtcaccgttg tatttgatgt ccattaaagc atcaatttta gtttcggtaa
     7861 caattgattg gttttgtgta ttgataacta caatgggaag ctcagaacca tcaaattgga
     7921 taattcctaa atcatgcgca cagagttgaa attctcccgt tccgccacca tagccaaatg
     7981 cttggatata gagcgtttgg ccgggaatga ggtcataacc tgccactaaa gaataatatc
     8041 cacctccacc atcatcgtcg caggaaatag cttgaagtgt aaagcatgag ctatcggttg
     8101 gaacccaaag ggcaagtcca gtatcattta aaccaccatt tgaggtattg aaatacacat
     8161 tacccgaagg tggaactatg agtttatacc aaatatcagc tccggcataa tttgcacatc
     8221 ctggatttaa tccagaaccg ggaaaattgg tgtttgaagt ttgaacgcaa gcaccagtat
     8281 caatgagaat ggcagtgcag gcgctcgttc cgggagcggg taaagtattg caatcgccaa
     8341 cattgttgaa tacgattcca gttgctggat tgggaccagc tgagtaaacc acattatatg
     8401 ctgcatcaat taaagcatag gaattttcct cttcgtaatc ggctgcggtg taaaataagc
     8461 ggatggaatc acccgtgcag acttgaaatg tatcgactgt tccattattg gatgcactga
     8521 aaattccgag tgattgattg tttcgaaata tctctaatgt ggcgccattc cagccatcgc
     8581 cgtagctatc gtgcatgttg atgctatagg tacagcattg agaaaaggca tttgaaaaga
     8641 atagaaaact gcaaagaaaa aggaggtaga ttttcttcat gaacgaattg gatgtttgca
     8701 atctaatgaa cccgaaaatt actggaataa aacaagaagg caagaaataa ataggtgtat
     8761 tgacttgtat gtttttatga aattgagttt tttaatgtat agcaaataca tgtcattgca
     8821 acgaggcaat ggcaacgaga aatattgaat cgtttagtga ctcgtacttg aaataattgc
     8881 tacttattgt aattaattga accttttttg agggagaagg attaatgtaa aaaaatgcaa
     8941 gcgaagcaga attgctttat attagcagtc aaattgctgc gtgatgaaaa aagttggtaa
     9001 taaaacgcaa tcatttaata ccagaagaag cttaaagtcc aaagctgctc aaacgggttc
     9061 tgtgaataaa tcgttagtct atgaagcggg tgtagtttat ggttcaagta ataaaatgga
     9121 acgggttaat gcaatacgag caggaattcc ttatgatttt attgaggtaa taagtaagcg
     9181 attaaacaat ccaataaaaa atgttttagc tttaattggt atacctcaaa ctacctataa
     9241 caaaaagaag agcgagcatt tactacttga tagtagaaat agtgaattaa ttattttgat
     9301 gaatgattta atagattttg gtttagaagt tttcaatcag gaagaggaga aatttcaacg
     9361 atggcttaaa aaaccgaatc tttccttagg tggaaattca cccgaaagca tgcttgatac
     9421 cattacagga atcaatgaag taaaatttag tttgaataga attgagtttg gaaacttcgc
     9481 ctaatggttg tttaccgtat tgaaagggaa aaatatattg atacaacttt atcgggagtt
     9541 ggagcttcat tatcggaagg ggttcgatgg aattctttaa atactcgaat tgtttatact
     9601 tccgagagta gggcattagc gttacttgaa gtttcagttc atttagattt aagtgaagat
     9661 ttacccaatg accgatattt aatcgagata gaaattcctg atgatgtatt gattcaggaa
     9721 gtgatgttag cggatttacc caaggattgg aatgctcaac ctcccagttt aattactcaa
     9781 gttattgggg atgattttgt gaggtataat gaatcggcta ttttgaaagt tcctagtact
     9841 ataattcctc aagaatttaa tttcttaata aatccgaacc atcaagatgc tgctaaactc
     9901 agggttttga atgcatttaa tatgcaattt gattcgaggt taaaattaaa aggctgatat
     9961 actttaagtg aaaaatagaa aaatgaaatt tctagttcgc ataaataaat ttatctctta
    10021 aaatctcatt tgctaatctt aatctatgaa ataaatccgg agcattcgga ctaattcaaa
    10081 tatatggttt tcatcagact taaagtataa actagaatag tgtttcagag ttcattgatt
    10141 gcgatgaagt gttctagcat ccgtttgacc tcacccccgg cccccttgcc actatgtggt
    10201 ctccttcctg ctttatagtg gtccctatgg ataaaggaac agatttggcg agatgaggtt
    10261 gtgataaagt gacatgttaa ttctatcttt taatgaaata aaaaagcgcc atcctaaaca
    10321 agaacagcgc ttaaaaaaaa gaatgaattt aatattataa ttctacgggt tttgctttta
    10381 catcatccgg aatatcatgg gttaatgctc ctaagaattt aacaatactt gttacttgtt
    10441 ctggatttag atctttattg ttttgggttt tagccatgat gcgaaccgcg tcgttcaagg
    10501 attttacact gccatcgtgg aaataagggg aagttttttc cacattacgc aagccacaca
    10561 ctttgaaaat atctttatca ctttctttct tcgttaaatc catcaaacca ttatctccat
    10621 tggtacttcc tgttaacgga cgataatcgg tgttcacccc aaatttcatc atcatcgttc
    10681 ctcctagtga aggtccgcta tgacaggttg tgcaaccggt ttccataaat aaacttaagc
    10741 ccgctttttc ttctgcattc attgccgttg tttcaccacc taggaattta tcgaaacgag
    10801 aaggagtaag caatgtacgt tcgaatgctc caatagctaa tgctacattt ttaaagctaa
    10861 ttgggttttt atctaccgga aatacttttt tgaatttctc cacatacata ggaacggcag
    10921 ctaatttctt ttccaatgca gcttcatttg gaatagccat ttcaacggga tttagaatag
    10981 gcattccggc ttgttcttca accgttttag cgcgaccatc ccagaattgc atgctatgaa
    11041 ttgctgcgtt gaatgaagta ggcgaatttc gtcctcctaa tttacctgca tcaccagtcg
    11101 aaaacgcttt gttatctact ccaaacgtac tcaaattgtg gcatgaatta cagctgttat
    11161 ttcctgtagc agaaagtcgg gtgtcgaaat agagcaattt acccaaggcg attttctctg
    11221 gactcatcgg gttttccaaa acttcggcag tggatggcaa tgcttgaaaa aaggtttgtg
    11281 ctttggttag caattcttta tcggctgcca aatttttagc attatcctgt tctgctggtt
    11341 gatgacttcc tgaacaggct accattccta cagaaatggc caacattggg aaaagcaaga
    11401 atttattcat agggtttgat tttactatca aattactgac tcaaaattgg tcgataatat
    11461 gagattcgtc agtgttttca aaatatattc agaaacaagt tcaatcatgc actattattg
    11521 aaatccacga gaaagatctc ttaccaatta attacaaggc gaaattaaat aacagatccg
    11581 caattggatg tttttcttca agtaatttcc aacttttttg attgaatact taccaaatcg
    11641 atagtgcttt cgtttcgggt agtgtattgc ggggattctt ttactggtgg acatttgcag
    11701 caaacaaaat ctaaaaacat gtttcagcaa agtgtattta ctcagtcatt tcatttgaat
    11761 caatctagaa acaaccattc aaaatttcta agctttatta ttggaatatt cttctattta
    11821 ggattttcaa ttatcgccca attatcagct cagaatttgg cgaaatttag tgttgttgat
    11881 atgcctgagg cttcgacgga tttatctaat cgtgaagtag ctattgcagt ttctcgatca
    11941 attcttcaag ctaattggga agaattagac aaattactcg acgacaaatt tacctatacg
    12001 ggtgatggat atgtgtttac gaaagatcaa tacattggat ttatgcaaga tatgcgtgca
    12061 gcattttcca atttcgaaat gattttggat aaatctgtca tgcagggaga tcatgtctcc
    12121 tttaaatttg tttctcacgc agtaaacaca ggcaaattca tgggtgcgcc tgccaataaa
    12181 aagcatttag aagtgaatgg aatctttatg cggaatgtga aaaacggtaa agtaattcaa
    12241 gaatggcaaa ccaccgattt attaggtaca atgaaacaaa ttggaggcgg tgctctattt
    12301 ttctattctg tttttgtagg tggatttaat gttcaatcag ctcctcctgt tcgcaaaccc
    12361 aacgactttc taaacataaa tgggaatgct aatacttacg accaaatgac ctccaagcaa
    12421 aaaaataaat acgtaaaaaa ataccttaaa gactggaaaa aggcaacgaa taaaaaatga
    12481 ttttatcgtg ctctttaaat cagctttaaa ttaattgtcg gggatcaatt aaatgcgcta
    12541 tcactaacag atagcgcatt ttttatttct caataatcac tttggattgc acaaatttcc
    12601 ctcgcttcgt ttctaagcgc atcaaatagg ttccggcagc taaatgctca actggaaata
    12661 cccaaccacg cattccactt ggaatcatca atcggcccat gcttctgccc gacatatcaa
    12721 acaactctgc atatcgcatg tcttctttat cccaggtaag cgaaacttga gtttttgctg
    12781 gatttgggaa tacctttaaa ctggaatttt cttctatcgt aattggctga atacctaagc
    12841 tgctatcgat ctcggcgaat gtatattcac ccaacatcaa actatctaat gtgaacgatc
    12901 cgtatttatc ggttgtgctc gataaagtgt ttttggttgc atcaagcacc gtccaatctg
    12961 aaccattatc ttttcgatac agcaatacca attttgtttc atcggtgatt aattcattat
    13021 ccaaccaacc acttgatgta cttgaagtgc gaccattata cgtgaatttt gcacttgctt
    13081 tccaaccatc gcgtaatacg ccatccacat gccagtagcg gtttggactc aatcgataca
    13141 tcgcaaaagc ttgtttaaat ggatcgggtg caacccaatt atgttctacc cgaattaaaa
    13201 ccgaatcttg aacagtactg gtattaattt ctaataaagc attcgcccaa ttggcatttc
    13261 cagttgtttt aataaaacgt tcttctgcag taactgcatg cgagatagct tcgttgcgat
    13321 ttaaggaagc ataaacgggt tcaaatggta ccgtaacatc tacaaatcca ttcggtccat
    13381 tcaagacaac ttgagcagta aacaattcga aattacttcc acgacaggtt aattcaaatg
    13441 gaacattcgt gtaggtatgc gtattaccag ccgatttctg actgtaataa aaacgattcc
    13501 caattccacc ggtaataatc atcgaatcga tctcaaattg tccccaaccg ggcgtatcta
    13561 cccaatcggc aaagaaatca tgtaatggaa cattggttgc agcttctaag gcatcccgca
    13621 agtcgtttga atttgcatca cgaaaggtgt aggtattgaa ataatgcttc aagcccacgt
    13681 aaaacaaact atcacccatg taggtgcgca atgtgtgaat aatgtcggat ggccgctcat
    13741 acgtggatgt ggtatacgta tacgcttgtg gtacatttga caaaggccaa taaccaccat
    13801 ctttcacgtg gcataaatta accatcgtgc ggtgattcgc tctcacttga gcatcgtacg
    13861 tagcgcggga aataagccat tcgtcgaaca atcgctcgca gtaagaagca aacccttcat
    13921 tcaaccacat ttcggaagct gtgcgacagg tagctaaatc accaaaccaa tgatgcgaaa
    13981 gttcatgtgc catgatgtgt tgattcccag cagctccagt agctagcaaa tcgcgtggat
    14041 agcctatgtt acttgcatgt tccatcgcgc cagcttgaaa aggcgtcatc acatagccaa
    14101 ttcgatccca acgataagga ccaaagtgac tttcaaattc gtggaagatt gcttctaaac
    14161 tcacgaatgc atttttcatg gcagttgtat cacccggaac agcagccaat ttcacattca
    14221 actgcggatt gagcatgctc gtatacgtgt aattcacttc ggtatagggt gcaacagcaa
    14281 cggaagctaa ataactcggc acttcctgat ttaaggtcca gtgacgcgta cgaaatccgt
    14341 tttcaaccgt gtctacttct aatattcctc cacaatatgc tttcaggttt tcgggagttt
    14401 tcacgatgag tgaaaaggtg cagcgttcaa caaaattgtc aaaacaagga aaccaaacac
    14461 gaccgaaatt atgtggatca gcggcgaatc caacgccaat gttataagca tatccacccg
    14521 aaaaactaaa acctccaaaa ccggatgcat cttctaccgg agtgccatga taaaagactt
    14581 ccaccgaact gttattgccc gtttgaagcg gagaaagtaa atcgatacga agttttgttc
    14641 ctgaatgatt aaaggtaaca gcttcaccaa attgcttcac cgaatcaacc gttaaggctt
    14701 gtaaatcgag tgtgatttga ttgatattat ccacacgagc actaaaattt acctgacaag
    14761 caccaatcaa attggctgta ggtaaagcgc taaaatcgag gttgatagtt gtgtgactga
    14821 tatcgatgct atcgctacgg gtatcaagtg ttgcggcatc tgcaacagat ggtccgccag
    14881 atggatgttc aatggaagca acttgtgctt gtactgttgg gaatagggaa acaagtaatg
    14941 ctgaaagaat gaatcgcata gaaaagattg agatttacaa aaatgcatga aatcttttag
    15001 aaaatggcgg ttcattctcg gctgtggata agttaaaact gccgtcaatt ctcttacttt
    15061 gcaaaccgaa attgagcatt catgagtaag cggtttcctt taggttggat gatgttagtt
    15121 ttacttgctg cagcgtgtaa gaaagatcct gtagataaaa ttgctcctca ggttaacgac
    15181 tttagtgtgc ctgccgatag ttccagcttc aatggaaatg cattaacttt tgaagcaaca
    15241 ttgagcgatg atatggaatt gagccaatgc aaagtgatta tcaaaccaac atcgggagct
    15301 tatattacta gtgatacact tgcattgcct tattggaatt ttactcgtgt gtatgacgtt
    15361 catagtttta atgacattat ttctgaaacc atttctattc ccgataccat tgccagtgct
    15421 tggtataata tacttttaag tatagtagat gcttctggaa atctaagtca aaccgatacc
    15481 aacgtcgttt ttattcgcaa taatgctgat tcaatcccgc caacgctcat ccttcaatgg
    15541 ccggccgatt tacaatttat aagtaaaaca ggatccttgc acattacttc cgatgcaagt
    15601 gataatcagg aactttctgc aatagtttgg aggattttcg aaggctcaac ccaaatacat
    15661 accgaaacca agtatttgaa tgccaatccg gaatcagttg aatttgattt ttccttgtcg
    15721 ggattgtcta acggactcta taccttcgaa ctaactctgt acgaccgaat gctgaattcg
    15781 tcaaaagagc agcgaaattt tgtggtgata gattagaatt agaaaagtgg ttctttcgaa
    15841 cgaatgtcgc gactaagtgc agcaagttcg atagctgtga ttgcagcttc atcgcctttg
    15901 ttaccatgaa ttcctccaca acgatcgagc gcttgctgta gcgtatttac tgttaataca
    15961 ccaaaaatta ctggaatatc ttgtgtggag cacaaggtag ctaagccgtt tgtaacacct
    16021 tgacagatgt attcaaaatg cggcgtatcg cctttgataa cggcaccaat gcaaatcaca
    16081 gcgtcggttt gactatgagt cacagctaaa cgagcggcgc tgattagttc ccaacttcca
    16141 ggtactgcaa taactcgtat gttttgttga ggaatgccat ttagaagcaa agtttcgaca
    16201 cacccttttt cgagttcgcc ggttacttct ttattccact gcgctacaat aacggtaaaa
    16261 cgaaaatggt caatattcgt gatattgacc attttacgag aagatagatt ctgatgtgca
    16321 gaagacatta ttttccgccg ttttctttca ttccttctaa acgagctatg tacttttcag
    16381 cttcacgacc ttcagaagtt tgaaaatatt ctttatgaac tttcgtgtag atttctaacg
    16441 ctttatcgta gttcttcaaa tcttcataag cgtaaccagc tttcattaag aacataggtg
    16501 tactaaagcc gttttcattt ttaccagcag ctttcacata ctgatcgata gcttcttgcg
    16561 tatttcctaa ttccatcaaa gcatcacctt ttgctcccat agccattgct gaagtcatca
    16621 tatcagctcc gtcgtatgca tcaaattgat caatagcttc ttggaattta cctgtttgaa
    16681 ggtaacaaac accagcatag tagtgagcta ggttgctaga ttttgtcccg ctataattat
    16741 ccatgatagc taagaaacct tctacttcac ctttaccatt caaagcaaga ttgaaagaat
    16801 ctttctcaaa atagtattct gccttaaaca tagcattttg agcttctgct tctttaggct
    16861 taacgatgaa ctgtttgtat gcgagcactc cgccaaccac cacaataatg gcggctagta
    16921 ccatcagaag tttcttctga ttgcgttcaa taaacatctc ggtattcgta agcgaagact
    16981 caatgtcttg taaagtatcg tgcttaggtg tggtcttatc ggccatgttc tttgtcaaaa
    17041 aatgaggcgc aaaaataagg tttttttgtt accaacaacc acagaatttc ttataagctt
    17101 agttccagtg tttcgcccgc tgttcgtgta tttcttgtgc aaatctcctt cgtttttgct
    17161 gaatactttt cttttctgct ccatctgggc gtgtatcttt gtcgctccat gcagctcagc
    17221 gaactccatt tggtcaattt tcgcaattat cccgaagccg atttggcttt tggacaatca
    17281 atcgtggttt ttaccggaaa taatggatcc ggaaagacga acttactcga tgctatccac
    17341 tatttagctt tttgcaaaag tttcctcaat ccaatcgatt ctcaaaatat ccgcaacgaa
    17401 gaaccttttt ttgttgttca aggttcgttt cagaatgaag aatcgaaaga tgctgtgtat
    17461 tgcggcatca aacgcggaca aaaaaagcag ttcaaacgca acagtaaaga atacgaacga
    17521 ctttccgagc acatcggatt gtttcctttg gtggtgattt cgccttccga tatagccctc
    17581 atcacagaag gtagcgaaga gcgtcgccgc tttatggata gtgtgatatc gcaatacgac
    17641 aagccttatc ttgaacattt aatccgctac ggaaaaatcc ttcaacagcg aaatgcttat
    17701 ctccggcagg gaaatagtca ggccgacatg ctcgagattt gggatgagca actagcacaa
    17761 tatgctcgtc cggtttttga agcgcgcaag ttatttttgg ctcgcatcaa gcctctgttt
    17821 caacaagtgt attcctttat aagtaacagt accgaaacgg ttgaacttac ttatgagagc
    17881 aagttgttcg atgctgatcc gtatcaacta ttgcagcaaa cacgagagaa agaccaaatt
    17941 ttacaataca cgacggctgg tattcataaa gacgatttgg gaatgcttat caatggtttt
    18001 ccgttacgta aatatgccag tcaggggcag cagaagtcat ttctcattgc actaaaactg
    18061 gctcagtttg atgtgttgag cgaggttaag caaatgaaac ctctattgtt gttagatgat
    18121 attttcgaaa agcttgatca agatcgtata actgctttga tgactttagt gagtcagcag
    18181 catttcggtc aaattttcat caccgattct catcccgaac gaattgctga aatccttcgt
    18241 ggaattcaag ctacctttga tcactttacc gttcatcagg gaacggtgca gtccgcatga
    18301 aacgtaaaag caacgattct tccgttaccg agctaatcga tttatggctc aagcagtacg
    18361 gcctcaatgg caagtaccgg gaatttcgta tgctgcaatc ttggagcgaa ctcatggggc
    18421 caatgattgc aaatcgcacc gatgatattc gtatttacga agggatatta tacatccaac
    18481 tttcatccgc cacgttgcga aacgaattaa gctttgctaa atcgaaaatt attcagtcga
    18541 tgaatgaaaa agcaggggaa gatttgatta aggatattgt ttttagatga tttgggcctg
    18601 cccatcgttt gtttcgtaaa ctccacatac tcaggtcgcg ctttcgggct attagtcctc
    18661 ttgtacgcct tcgctatcgc tatggcgctc aatgcgggct atccgcctca atcgctcagg
    18721 cgatcaacaa tttcatttaa caatctttcc aaccggataa atcaaatacg ttttatccgc
    18781 ataaggtgat tgctcataaa accagcgtaa ttgtgcttgt tggtcttttg caaaaacagc
    18841 atcgctcgac ttcttgcttt caaaagctga ctttagagtc ggatttttat tgagttggtc
    18901 ggctgcgtat ggctccaaca cgtatgtttc gaaatactcg gttcgctcta aacattcggg
    18961 gaaaaagccc cattggaagt acgaatcggt aaactccggt tcgagcaata aaacagctaa
    19021 atcgcccaat gcctgattgg tagatactag catcgaacct ttcggaaata cgacttgcat
    19081 cgattcttct ttcgcatcag cttttaagcg gcatcgtccc tgattaggca gcttaccttg
    19141 aatttgagga ttactcaaac gatacatgct cagttgaatg gttgagtcgg tttgaatgct
    19201 ttgcatttca agaccatgtt gcttcaaacg agaaataact tcttcgaatt ggaccggaat
    19261 tacataatac gacgggcgag ccacatgtga agttgctaca ttgtttttcc gtaccggaat
    19321 gtgctgattt tctgctttcc ccgaccattc aacgtattct actcccgtta cttccgactt
    19381 tacttttttt gatgcaattc ctgcaaattt catcgaatca cttgcggttt tagggaaatc
    19441 ccaagtcaag ggtaatgctt cgggttttcg tttttgatcc gatgctattg cagtattcaa
    19501 cgaagttcct tctaaactta atacgcgcag tgtttcaaac aataaaacat aggttcccaa
    19561 cactcgctga cgaaaaggtt ttaacgaatg attttcaacc aaaatgcttg gtaaatgacg
    19621 ggcatcaccg taaccatgcg aatagcgagg cgaaaacgtg tattcgttgt ttccatcttt
    19681 aaaatcatca tcattcacag caaaaagtag cggtccggga atatgattat tgctcgtaag
    19741 tgccacgtca acctgtgaac gaaatttcgt ttttaaccag gttgaaatag caggagaata
    19801 cgcatgttct ccttgaaatc cataggttat atcgtattga taatctgcgc catccgtcac
    19861 atgtatatcc atatacaaaa ccggattttc gcgattaatt actgctaaca ccgactgtat
    19921 ttctttcgtg tcgagtttgg tataatcgcg gttgaggttt tggttgagtg cattacttct
    19981 ccagcccata acggttggtc cacgctgatt tatccgattg aattcggatg ctcgttcatg
    20041 tccatctaca ttcaaaattg gaatgaataa tacattcacg ttttgcagca attgaggttg
    20101 tatttcaagc acatcgcgga agaataacat acccgcatct ttcccatcaa tttcacctgc
    20161 atgaatgcca gcttgaaaca gaaaagttgg tttttccttc cctgcacgtc gaatagaata
    20221 catccagata tctcggtttt gagctgattt accaatactt tcctgcttaa catcagggaa
    20281 cgttttctgc agtttttcaa accaatctcc caattcttgc ttggtagctg agttcattcc
    20341 gccacttagt tcaaatggag taaccaatgg atctttatta gaacgcataa tccgctcact
    20401 ttttcccttc caggctaggg taggaggaag gtaagtttga gacgaaacga cttgtgtaag
    20461 aagtatgagg acaagcaggc aatatttttt tgtcaaacgc atagttcaaa taaacaaaaa
    20521 gccacgcaca tcagccactt gagtccattt attatttgca tgctagtttc tctacttaga
    20581 aagttcgaat cttatttttc aaacaaaacc atcctaaaat tggaaaccta ctatattaat
    20641 atagcgttaa acaattttgt tattattact tttgcaaatc ctaaccttaa agtgtgctga
    20701 aaatagctaa gtcgctatta ttacttttta tttttattct gctacaaatc ccagtatttg
    20761 cgcagcttac gctgggcttc aattcaaatg ggaatacagc tgggtgtgct ccgtatacat
    20821 taacatttcc gattacaaac attgccggta atccagctaa caccacttat gtggtcaatt
    20881 ttggagatgg tagtcccgtt ttgaacttca cgcaaggaaa tattcctgcc tcagttacgc
    20941 atacatacac caccaattcc tgtggttccg ctttccaaac taccaataat gtttttggag
    21001 caacaatcac agcttcaagt cctacgaatt cgcccttcac ggctgccgtt tcgcctatcg
    21061 taatttcgtc tccacccgat gcgattatct ctccattaac ctcaacagtg tgtgttggct
    21121 catcggtttc gattaacaac aattcctctc aaggtgttta tatcgaccca agcgccggat
    21181 atacttgttc caccacgggt gctatttctt actggactat ttcacccgct gcaggttggt
    21241 cggcaagtgc agccagtatg ggaagtaaca atggtttccc aaatgcatcc gatttcgatg
    21301 tgtggtcaca gggagcaact ccattgagtg taacgttcaa cacggctggt agttatcgag
    21361 tgaaaatttg gattgccaat acctgcggac tcgataccat gtcggttcct gtgtgtgtgg
    21421 ttcctcctcc caatcctcag tttaatctaa acccaagttt tggttgtatt cctccgaatt
    21481 tgagtgtgac agcaaacaac acaactgctg ttacaccttc ttcttgctat acgccaactt
    21541 attcctacaa ttggagtatt tcaccttcta gtaattgggc atttaatggt gcaagcaacg
    21601 ccagttctac ttctccgtca ttttcatttc tctctgccgg aacctatacc gtcacgctaa
    21661 cggcaagtat cagcgctctt tcgggttgtt cagcttctat caatcatcca gtaaccgtta
    21721 atcaagcccc aactgtcaat gcaggtttag atcaatcggt ttgtgttgga tcacctgcca
    21781 ttcaattaac gggttctcct acaggtggta catggtcagg cacaggtgta aatgcgtctg
    21841 gattattcac tccaacaact actggaaact tcacgcttac ctacaccatt cctgcatcag
    21901 ccggaacggg ttgtcctgcg gttaccgatc aagttatcgt ttcggtcatt actccaactg
    21961 ctgcaaatgc gggagccgat caatcgcttt gtcccggtac tcctgccatc caacttaacg
    22021 ctactccaac aggcggaacc tggaccggaa ctggcgtgac ctcaggtggt gtatttaccc
    22081 ctacaactcc aggaagctat acgcttacat actcattcgg tacaggacca tgtgcagttt
    22141 cggatcaaaa agtcatcacg gtttattcac ctccaacggt atctgtaaat agtcctgtgg
    22201 tttgtgctgg atttaatgca acgcttactg ctgtaggtgc agctggttta ggaccttatt
    22261 cctatgcttg gtcaccaagt gcaggtttga gttccggttc tggtgcatct gttaccactt
    22321 ctcccgcgcc tttatcttct acaagctaca ccgttattgt aaccgatgca catacttgca
    22381 cggctagtgc tgtctccagc ataactgtta atccgctacc atccgttgca gccggttcag
    22441 atatttcggt gtgtaacaac gctcaagtaa ctcaacttac tggaactcca actggcggaa
    22501 cttggtctgg aactggcgtc acagctggtg gtgcattcac acctcctgga ttaggaaatt
    22561 acacgctcac ttactcgtac actaacgcaa atacttgttc gtcgagcgat caaatcatag
    22621 taagtgtggt caatccgagt cctgctggtg ctggctcaga tcaacaaatc tgcgttggtg
    22681 caacaccact tcaactcaat ggaactcctg caggaggaac ttggtcgggc gctggtgttt
    22741 ccacttctgg actattcact ccaaccacac cgggtaacgt caatttgaca tacagttttg
    22801 gttctggaac ttgtgcatca accgatcaga tgcaaatgac cgtttatgct gctccaactg
    22861 taaacgtgaa taatccaact gtatgtgaag gcacgcctgc aactcttacc gcagttggtg
    22921 caggaggatt aggtccttat tcttattctt ggaacccttc agctggatta agtgctgctg
    22981 ttggggcaag cgtagttgcc aatctaacta caacaaccag tatcaccgtt acacttaccg
    23041 atgctcatac gtgtctagca acggatgttg ctttggtgac agtcaatcct gttccggttg
    23101 ttaatgcggg tgctgatcaa acaatctgtc taaatccttc caccatccaa atgaatggaa
    23161 ctcctgctgg aggaacttgg acgggtgata acgtaacaac atctggagct tacacacctg
    23221 tcgcagcagg aaccgattcg cttttttaca ccatctctac ctcaggatgt actcgttctg
    23281 ataccatggt agtgaatgtt caagttcctg gatctttgca acttacacct gatacaaccg
    23341 tgtgtcttaa tagcgggaat tttcagttgt atgctgcaac actaggtgga aactggacta
    23401 gcagcacagc cactttaact tcgagtggaa tttttactcc tgatgctgtt ggcgtttttg
    23461 tcgttagcta ttcggttgga gcaggatttt gcgccgtggg cggaagtctt caagttactg
    23521 ttattgctcc tccaacggta gatgctggtt cagatgtgac actttgtgct aattcaccag
    23581 catacacgtt tccgggtgaa agtccgactt ctggtggaac ttggtcatgg tcaggaacgg
    23641 gaatcacaaa cactaccaca ggagttttta gtccgagtat ttcgggagca ggagtttttc
    23701 caatcactta cacattcact tcaacagcaa ctggatgttc tgcttcggat ggcttgcaag
    23761 taactgtgaa cgcacttccc aacatcgtac tcaatcctgc tgtgctcaat gtatgtttaa
    23821 cgccttttgg tagtcagtta acagctagtc cggcaggtgg attttggact ggtgctggac
    23881 ttacatacgg ttcaaacgta atcgctccgc aggatacagc tacgtatacg ccaagtgcag
    23941 ctggttcatt ttcggtttat tatacctaca ccgatgcaaa cacctgtgtg aatcgagata
    24001 cggtgttgat taacgtaagt gtaccaacac ctgcgaatgc tggttcggac acggcatttt
    24061 gcttttcgaa cttagcgcat caattgaccg gtacacctgc aggtacttgg attaatccta
    24121 gttggctgaa tgcgaatggt acgtttatca gcgatactgt tggtacgttc aatgccattt
    24181 atgctgtggg cggaggttct tgtttggtgt atgataccgc attagttacg atacatccat
    24241 tgccagttgt gcatgctggt ttagatcagc aattgtgtgc tgccgatccg tgtttcaatt
    24301 tagctgcgcc ttctccactt ggtggaactt ggtcgggacc tggaattgcc aatgctgcaa
    24361 gcggacagtt ttgtgctgca acagccaaca atggtgtgaa tcaaattctt tacacctatc
    24421 aaaatccaac tacaacttgc tcgaacaccg atactttgct agtgaccatt gttccaatgc
    24481 ctgttccggg tgtagcgatc aatccgctat tctgtatcaa tacacctgtt caagtgacga
    24541 atttaagttc gggagcagct gcaacttttg agtggaatgt gaaaaatact gtaactggcg
    24601 cattggttca aacaagtaca ttggcttcac cagttgtgaa tattgctact gcaggtacat
    24661 atcaaattga acaaatttgt tattccatct ataactgcga aacccgagat acagcatatt
    24721 ttaccgttgt tgctcctcca atagcagcat ttagtttagc cgataatatc gtgtgtggtc
    24781 cgcatcagga aacggttacg aatacttctt ccggttatca gatatcattc ttgtgggatt
    24841 ttggtccatc tggtccgggt tcaaccgata ccattccggt tttacctgtt tttgcagctc
    24901 ctatcattgc cgattcgctc tatcatgtga cgctttcggt aagcaatatg tgtggtacac
    24961 gaacggctgt cgattcagta attattcgtc ccgttccagt ggcacttatc ggaaccgatt
    25021 acagtcaagg atgttcgcct ttttctgctg tgattcaaaa tgtgagttac ggttcacctg
    25081 attggtttat gtgggatttg ggtgatggaa ctacatcaac cgattcactt ccgccagctc
    25141 atacctacac aacaaccgac accgttagct tcattccaat cactcttcaa atcggcaata
    25201 cctgcggaaa tgcgaatgct gccacgaatg tgattgttta tcccgatcat atcaatccaa
    25261 cagctcagac tcctattgtt ggatgttccc cttttgaagt agattttaac ttcccattag
    25321 gtgatttgac ttactatctc tgggattttg gcgatagtac aggcattgca ggagattcag
    25381 tttctcacat ttatgaaaac gctggtgtat atcatgtcat gcttacggtt tcgaatttct
    25441 gtttggttga taccattttg aatactgtta cagtttatga aggtcccgat ttagatttta
    25501 caatcgatca accttccatt tgtcagacga accaaataca aatcagcaat acatcacaaa
    25561 acagtgcttt tgtcaacatc gattttggtg atggaagcac aagtccaatt agctcatctg
    25621 tgaaccatgt gtacaattca acaggtacac aaacaattca gatgtatgga gtgaatccac
    25681 aaactggatg tgtggataca gttagtcaaa cagtggaagt tgttccattt ccagtgatta
    25741 atgtaactgc agatccagat actggatgta tgccattgct tgtgcagttt ttcaattcaa
    25801 gttctttagc tacaggatac gaatggcact tccctgatgg aacaagttct atccttgcag
    25861 aaccacaaat aatgttagca caatctggag actttcaagc tcaactgatt gctcataatt
    25921 accaaggaat tggtgctgat tgccctgaca cggcttatgt tacaattcac gtaaatcctt
    25981 ccccacaaag ctcgtttatt ttagcggccg attctgcatg tggaccacca gctcaaactg
    26041 ctgtaagcaa cacatccaca ggaggaaatt cgtatacatg gctttgggaa tcgactagct
    26101 caaccgaatt tgaacctcaa ttaacattta ccgatactgg tttccatcaa gttcgtttag
    26161 tagccgaaaa cttgtttgct tgtcgcgata cttccgcttc tggtttcatg gttttaggac
    26221 aacctcaaat tgctttgtca atcgatcctc cgaacggttg tatgccacac gaagtgacgt
    26281 tcgataattt gagcctatat ggtaatcaag ttcaatgggc atttggtgat ggacaattct
    26341 ctacgctcga ttcagtaagc catgtttacg aagaagcagg attgtattcg attgaattac
    26401 tcgtctcttc gggtggtatt tgttccgaag attctatttt ggttgaagtc attgaagtat
    26461 atccaaaagc atttgccgga ttggaaatta gtcctcagtt tatttcagaa tcacttcctc
    26521 aaattaatct aaccaacacc agtgctttag ctactgcata tcaattgtac gtcgaagatg
    26581 atttagttag tggaattgtt ccaccaacct atatgtttga aaatcctgat acaggactcg
    26641 ttcaactcat gctcatcgct aataatatgt tcaattgtcc cgacacagca tatagtgatg
    26701 tttatatcaa atcatctcct aatatctata ttcctaattc ctttacaccc aacgacgatg
    26761 gattaaacga tggcttccgc ccctacatgg atcgaactcc ttcggaatat tactttgcca
    26821 tttatgatcg ctggggtcat gtagtatttg aaactaacga tagggataaa tactgggatg
    26881 gaacattcaa aaacaaagga aagaaagcca tcaaacaaga tgtgtatgtg tataaattga
    26941 cggctatttt tagtgaggaa gaagtgttta atttgttcgg aaatattact gtaatccatt
    27001 gataaataaa tcgctacata cacg
//
LOCUS       DAOOQW010000012        26843 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_2145_length_26843_cov_5.926721, whole genome shotgun sequence.
ACCESSION   DAOOQW010000012 DAOOQW010000000
VERSION     DAOOQW010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 26843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26843
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2145_length_26843_cov_5.926721"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="PK281_01660"
     CDS             complement(<1..618)
                     /locus_tag="PK281_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48074.1"
                     /translation="MRTGRLLLLLFGLLLSSVLQATHNRAGEITYRHLGGFQYEATII
                     TYTKSDSPADRPDLGISWGDGSVDTIPRINGSGMGELVAPSIKKNIYVGVHTYPGPSI
                     YTLSFEDPNRNGGLVNIPNSVNIPFYVSTQLVINPFLGVNNSVQLLNPPIDEACAGQI
                     FIHNPGAFDPDGDSISYRMVDCRGEDGLIIPGFSQPQASSSFTLNA"
     gene            complement(732..2933)
                     /gene="recQ"
                     /locus_tag="PK281_01665"
     CDS             complement(732..2933)
                     /gene="recQ"
                     /locus_tag="PK281_01665"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007093793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HOY48075.1"
                     /translation="MLEAVDTTIDLTGHLKKYFGFSKFKGEQEAIIRNILDGNDTFVI
                     MPTGGGKSMCYQLPALISEGTAIIVSPLIALMKNQVDSIRNFGGATGIAHFLNSSLNK
                     AEITRVREDITNGVTKLLYVAPESLTKDENVQFFREINISFFAIDEAHCISEWGHDFR
                     PEYRRLRSIIEAIGTVPIMALTATATPKVQQDIMKNLNMVETTLFKSSFNRSNLYYEV
                     RPKKNVVKEIIKFIKQHPGKSGIIYCLSRKKVEEIAETLNVNGIKALPYHAGLDASTR
                     ASHQDKFLMEEADVIVATIAFGMGIDKPDVRFVIHHEMPKSLEGYYQETGRAGRDGGE
                     GVCLSFYSFDDIQKLEKFLKNKHVAEQEIGQQLLAETVGYAETSMCRRKYLLHYFGEE
                     YDESGCNNMCDNCRHPKKKFDAMEDVCLVLDTVASLKGKFKDKHINHILTGTIDSVIT
                     SYKHDQLELFGKGSEHSTLYWSGVIRQAVVMGLLEKEIENYGTLHLTSVGTEFLENPQ
                     PVMITEDHDYSDTDDEDELAAAGQGAAAADEVLFSMLKDLTRKIAKRMNMPPYVIFQE
                     QSLVDMTIQYPVTLDELKQIAGVGAGKAEKYGKEFVETIAQYVQENEIDRPLNMVVKS
                     VVNKSTQKVFIIQSVDRKISLEDIASSKGLTLVSLIEEMEAIVYSGTRLDISYYIDDV
                     IDEDIQDEIMDYFRKAETDSMAVAIQEMGDDEIGETELRLMRLKFISQFGN"
     gene            3391..4662
                     /gene="kynU"
                     /locus_tag="PK281_01670"
     CDS             3391..4662
                     /gene="kynU"
                     /locus_tag="PK281_01670"
                     /EC_number="3.7.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030429 - kynureninase activity [Evidence
                     IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HOY48076.1"
                     /translation="MSQEFSSEQAAELDQQDELKIFRSRFRIPTLQGKEAIYLCGNSL
                     GLQPKAVADSIQVELDDWAQLGVEGHVHGRRPWLSYHEQFAAPLARLVGARETEVVAM
                     NGLTVNLHLLMVSFYRPSSSRYRIICEEKAFPSDSYALNSQAALHGLKPEDVIREISP
                     RSGQAHIELSDVLQAIAEESDRLAMVMIGGVNYYSGQVFDMAAITQAGHAVGAMVGFD
                     LAHAIGNIELNLHDWEVDFAAWCSYKYLNSGPGSVAGVYIHEKHASNPNTFRLAGWWG
                     HDKASRFKMEPGFNPIPTAESWQMSNAPVLSMAAHKASLDLFDEAGMPALIAKSKKLT
                     SYLHKLLLEVQQLPGFEGMFSIITPDERGCQLSLLFHREGKSVFDHLFAHGIIADWRE
                     PNVIRIAPVPLYNSFTDAFQFSKILSIYQSN"
     gene            4668..6005
                     /locus_tag="PK281_01675"
     CDS             4668..6005
                     /locus_tag="PK281_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008612222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOY48077.1"
                     /translation="MSKPKKITIAGSGLVGALHAIYMKRQGFDVDLYERRPDLRNNRI
                     SAGKSINLALSDRGFRGLAGVGIEDDIRSISIPMYRRVMHDTKGNLTFQPYGKDGQAI
                     YSVSRGGLNCMLMDLAEASGVKIHFNYNLLSVDLNSARTTFVGEDGMVKIDADFLVGA
                     DGAYSEARNELEKTPWFNYSQYYIEYAYKELSIHPNNDGSHKLEKNALHIWPRKDFML
                     IALPNLDGSFTLTLFYPKTGERSFDSLDTIEKAEAFFRENFSDALDLMPHFREEYASN
                     PAAAMVIVRCFPWSWKDKMMLIGDAAHAIVPFFGQGMNCGFEDCFVFNELLNEHEGEW
                     GDLLRKYEIARKPNGDAIAELALNNFIEMRDRVSDPKFLLQKKIEARFSDKYPTKWMP
                     LYSMVTFSHIPYSEALALGKKQEAIMEKVMKLSDIESKWDSEEVEKLMLEHLN"
     gene            complement(6002..7234)
                     /locus_tag="PK281_01680"
     CDS             complement(6002..7234)
                     /locus_tag="PK281_01680"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOY48078.1"
                     /translation="MSRKEYSILLTLAFIQFTHVMDFMIIMPLGNQLMRLFNIEPQEF
                     GFIVSAYTITAGIVGFAGAFFVDNFDRKKILLVCYTGFLVGTLACAFAPNYAFMLAAR
                     IFTGAFGGLLSTLILSIVGDVIPNERRASAMGMVTAGFSLASVFGVPFGNYLASIYSW
                     HAPFLFIAAVGLVVAFFIFRFIPSINTHIQDKSTRPGFKTVINNLFNNSNQRKALLLM
                     SLLMLSQFSIIPFIAAFMEKNVGFTQIEVTYIYLFGGLCSVFAAPMIGKLADKVGKYK
                     VFAIFAALAIIPLFLITNMPVVPVWMALIVTSFFFIVISGRVVPAMALITSTTQPKNR
                     GSFMSFNSSVQQTSSGIAAFIAGAIVTEDATGKLQHYPYVGYIAIVACVISIWAASRI
                     MNADDYEELQAKSQKEAA"
     gene            complement(7415..9229)
                     /locus_tag="PK281_01685"
     CDS             complement(7415..9229)
                     /locus_tag="PK281_01685"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY48079.1"
                     /translation="MNKSTFFFILLTCLFSITKSQAQQHYLIDKTFGTTCGLTTFDDV
                     VNYSPREIFKTNENKYLIVGDLVDGAANSYNIFFMQFNPDGSIDSTFGLNGVKRWVFS
                     QRNTVNCILKQGDKYYVCGNEAPDNGYSSIRAYVARFNSNGNPDSTFHGNGHVVELTS
                     TNSVSSSFYTQLLAQEDGKVVAYGMETGNINGGDTKNIARRYLTDGSVDPTFHPIVVY
                     PYPSTPLPINGYSSPGIYDTDGSIRFVYASDFSPARIVSIKLDSLGEFVPTYGTNGVL
                     TTNIPASNVNLNRSFSGDNGTIWGYNSHTNVTSDLQIFKLDETGAPDSSYSSDGINDL
                     IDYPGTNNDEAGHFMYQDSQNRFWCMGSGSFAFGQTKGIIYRLNDQGNYDTSFEGTGY
                     AAFTELNGTTFRCAYFENDDELVVGTLSIGYVGITRIKLIEEILQIQNVGPDTICVGN
                     QTLLTIIQPSECFTYQWKKDGVDIGVLNDTTLTATESGTYSLSATDAVNTLTSSSISI
                     VVVDCQSIENPANSVDFSLFPSPAQENLSIVWAKSQQAKQAKLEIIDLSGRIVQTENL
                     KINPNQNHQINLGNLNAGIYLIRMTSADKTSTTRFIKN"
     gene            complement(9404..10045)
                     /locus_tag="PK281_01690"
     CDS             complement(9404..10045)
                     /locus_tag="PK281_01690"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOY48080.1"
                     /translation="MKKTRVIIFDDSAPRRDSLRVLISLYPDLEFCGAFEDCSRVVEV
                     IDETQPDVVLMDIEMPNVNGIQGVSIIRKKHPELAVIMQTISEDDDQIFESIMAGASG
                     YILKKASPDKIIEAIRDAVAGGSPMTPAIAQRVLMFFKQQVKNTPENDYTLTPREIEI
                     LALLVDGLSYKMIAAKADLSYHTVNAHIRNIYDKLHVHSMGEAVAKALKERLV"
     gene            complement(10081..13182)
                     /locus_tag="PK281_01695"
     CDS             complement(10081..13182)
                     /locus_tag="PK281_01695"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HOY48081.1"
                     /translation="MVGLVGSLKFIQNNFLGKNQAISHLFKIAVSIWLLFISNNLRSQ
                     SVSEFYQRFSINNGLSSNTCNTLLEDSKGYIWIGTSEGLNRFDGHQVKQYFHNPADTT
                     TLCGNTIKCLAEAPDGKIWIGTYGSGIGVFDPIKETFEHYQLQSDRFLTTLSNRINQL
                     IVNPDGSVLCVTEGGMFLYTPSRPVFKLVTIPGAKRKQSGFQAFLLGGPQYQGLWFLQ
                     GGQIAYFEASTGKFYSSMYNPKNWEVFTREWHSIGAIGEEKTIWIIDESTNHICSFNP
                     QKNQFEDWGITCLVGEKTPHKMHYSQDHKALLFSFWDEPPFFISIPKRNRYTEPFTLE
                     YPGSIGNVKANFILHDSRNIEWIATSNGVFARNAKTQNNELHWFNDTPDDWSIKSLVA
                     DKDAIWLIATDGIYRNDLKTEIRQKLSLPDIEPKSLSCLYAVPGTSMLLAGHSEGLIQ
                     IDRNNLRVSKFREFKDTSAFGHKRDILQFVHTDSKGRLWAGTWRGDLVGFATWDGPVF
                     YHSTSKKSSKNWPNAGLISIYESSQDLFVGFNGGAGVWKWDESSKTFSEYLTTQRFPR
                     FNGVVDALYADSVNLYIGTHGGGMCIYDFKTKTMNYLSRHTGLIGDYVYRILDVGKGN
                     LLFTTNNGIGTLRKSDLFVKAVNQDFMQDVPLFSSSGYKGPDGRIWFFWKTFFYGFKS
                     QYLNQLVQKPVITSFRLLDKEIGIPSTGELAFNYKQNFFTLEFSSFPFLYKDRIEYAS
                     KLKGVNDDWIISQSKNYADYTDLQGGEYDFQIKARVDGSAWSDVTHLKITIAPPYWKT
                     WWFRVLALLIVAGIVYGIYWYRLNQLKKMQHVRNSISSDLHDDVGASLSSINIYSKVA
                     MERMQASPETVNELLNQINKNASETMDRMSDIVWSINPNNDTLQSLFNRIKVHCMEVL
                     QASDMEVHYVNELANDMHIGMNARKNIFLISKEIVNNAAKYSEASELKISVKLKNDTL
                     ILLFSDNGKGIQHVETKGGNGFRNMRNRVTELQGEIAIESEIGLGTKITIHLPVARIR
                     EAK"
     gene            complement(13189..15306)
                     /gene="feoB"
                     /locus_tag="PK281_01700"
     CDS             complement(13189..15306)
                     /gene="feoB"
                     /locus_tag="PK281_01700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HOY48082.1"
                     /translation="MEDKPIHIALVGNPNSGKSTLFNALTGLHQKTGNFPGVTVEKHT
                     GQVRMFHPKDKSRRTVNFIDLPGTYCLFPKSLDELETFKVLTDHRSPDFPDMVLIVAD
                     ASNLKRSLLLCLQVIDLGLPAILAVNMIDILERSNLKLNTVRLAAELGIPVIPVNARD
                     KKGISEIKQAIFQEQVGAMKTFDNPADYHPGVMEKLQKSTGISSAYEAYLTAANYDLL
                     QSERTLPDLRKLLAEDELTPFGLQSEESLRRYGMIQTILDNCLAQVTITRALRFSMKL
                     DDVFTHPVWGFLTFLTILFLLFQAIFSWAEYPMDLIDSLFQKSGSWIGETLPAGKLND
                     LITQGIIPGLGGILMFIPQIAILFAFIAVLEDSGYMARVSFIMDRLMRKFGINGKSVI
                     PLISGVACAVPAIMSARTIKNWKERLITILVTPLMSCSARLPVYTLLIAIAVPDKHIG
                     IFNLKGLIMMGLYLLGFLAALGTAWILKWIIRSRERSYFIMELPVYRMPQWGVVAHTM
                     GEKVWVFIRDAGKVIIAISILLWALSSYGPGDRFEQIEKKYTSPVMIEQMGEEQAANA
                     ISSEKLRNSYAGILGRTIEPVIAPLGFDWKIGIALVTSFAAREVFVGTMATIYGAGSD
                     DDEATLRERMMNDTNDRTGLPLFSPAASIALILFYAFAMQCMSTIAVVIRETGSWKWA
                     IAQTTYMGVLAWLSAFIAFQILS"
     gene            complement(15313..15543)
                     /locus_tag="PK281_01705"
     CDS             complement(15313..15543)
                     /locus_tag="PK281_01705"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HOY48083.1"
                     /translation="MSADLSHVPIGESAIIRGFTDEEISLKLIELGCIPGTRITMIRK
                     APLGDPIAFACSGSLISIRLLEASTVLVEEHA"
     gene            complement(15631..15780)
                     /locus_tag="PK281_01710"
     CDS             complement(15631..15780)
                     /locus_tag="PK281_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48084.1"
                     /translation="MRKLLPLFVLIAVLVTPSCKLFKKKCDCPDHHKKKRIAMVQKHL
                     NPSFC"
     gene            complement(15815..16432)
                     /locus_tag="PK281_01715"
     CDS             complement(15815..16432)
                     /locus_tag="PK281_01715"
                     /inference="COORDINATES: protein motif:HMM:NF015411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HOY48085.1"
                     /translation="MNIAVIGTGNIGGRLAKVWAHAGHRIYLGTRNPLNDSIRSLVQL
                     NPDLISAHAPEVAASKADIILLAIPADATYDVVKQLGNIDGKLVIDAMNAVFRKPDGF
                     YRTSEAILAACGNDHIVKAFNMIGAENMDNPKYGDQVADLFICGRYPEDKQTVLQLAT
                     DAGFRPFDVGGPEMELPLENLAMIWGKLAFVSGLGRDIAFKVLQR"
     gene            16507..17214
                     /locus_tag="PK281_01720"
     CDS             16507..17214
                     /locus_tag="PK281_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48086.1"
                     /translation="MRYVLSFVILCISGFVSLAQTDNMGVGTLTPDPSAKLEVSSTSK
                     GFLAPRMSSVERLAILNPAQGLLVFDLTTNSFWYFNSGTWTELITQIPAAPSAILFSS
                     FSQTGIAGSAEAIMGSYVIPANTLDANGQAILVHAFGLLTTDTALVRLKFSGIELPFQ
                     TYGQGFWELTCRVYRTSANTSKCAGTLVVNGSSSTFVSQPAMDFSAAIPFQITGQQVQ
                     PVVNGVSLEGFEITRIR"
     gene            complement(17215..18174)
                     /locus_tag="PK281_01725"
     CDS             complement(17215..18174)
                     /locus_tag="PK281_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HOY48087.1"
                     /translation="MEISEDILSRIDLLQKKYAAMGQDLRSYLDGLLHADFLTYWDYI
                     HLDTLLSLQNPRTSFPDEEIFIMYHQITELYFKLSLHELKQIGAQSEPDLKFTINRLK
                     RVNRYFMNLTHSFEVMVDGMDREQFLQFRMSLLPASGFQSAQYRMVEIFATDFIHLVD
                     KDHRSKFEGTQASITEQFKYIYWQAGATELATGKKTLTLEQFEKKYSKQLIELAESNV
                     QSNIYAVYRKLERSGADLSELKQVMRAFDILVNINWPLSHYKSAVRYLQRDPEDIKAT
                     GGTNWQKYLPPRFQKRIFYPELWSQAEIEEWGKGWVEDVLAGK"
     gene            complement(18274..18837)
                     /locus_tag="PK281_01730"
     CDS             complement(18274..18837)
                     /locus_tag="PK281_01730"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HOY48088.1"
                     /translation="MKDLIRCSWCTKTDNMREYHDTEWGIPVHDDRKHFEFIVLDSFQ
                     AGLSWQTILNRRAGFRNAFAGFDPKKVAKFTDDHVAMLMEDEGIIRNRLKITGTIKNA
                     RAFLEIQKEYGTFDAYIWQFTGGKTLDGKRKSTSEIPASTPESDEMSKALKKRGFTFV
                     GTTICYAYMQAAGMVNDHVKDCFLKKK"
     gene            18961..20478
                     /locus_tag="PK281_01735"
     CDS             18961..20478
                     /locus_tag="PK281_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48089.1"
                     /translation="MLRTLIGVFVLVGNALLAQTSSLISQDALFQIPGRELPESSDLS
                     AKVRGSISPDSIFFDVEVTDDQIQANTAGTGDRIEIWFGSPWVNFSDYIIGEKGKKTF
                     IFRNTAEAGDDANLERFIRDADYPKGKLKDAETGKDVKPVVPDANSLRREYVFFGMTR
                     FGLSASQQPAKHLDRDKYGPFEKQTGLTLDDLSPFVNYTVTKTATGLHYHVAMHNKCL
                     GFGRVASMNKIRFVVDVIDADPGEVKEQVISTTSNRFYGRPYYFNQTELAFPLNILPS
                     YVPAHILERMQINLDVIYSGGTWKTFGWNSGGIVYAKEFVSEALTEFYFYTIDISYQK
                     IPEGQQVEHERVELKYDDVSIFPQHEVYLLLNNEIYSGKDYRYTGHLDNNFFNTIFRL
                     PDGSTAMVLYDYEVVDPLGFGQFGHTADEFIFIERVVGKEMSPLINIGQRIEAAATLV
                     VGENSQFKVTDVKSVHYAWVEFGKLFEVRIKGKTSESSRTLRYRINEEGKIVDSK"
     gene            20600..22741
                     /locus_tag="PK281_01740"
     CDS             20600..22741
                     /locus_tag="PK281_01740"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HOY48090.1"
                     /translation="MTSQQVVRADEGMWLPLLLKRLNYEDMKKKGCKLTADEIYNINT
                     SSLKDAVVMLSGGSCSAEAISSKGLLLTNHHCGYEAIQTNSSVEHDYLTDGFYAKSLS
                     EELPSGGITASFLIRIEDVSQRILAELNPAMSESERTAKVRQLYKTIEKEATEGTGYD
                     AAVKSFFDGNEYYLFVYETFTDVRLVGAPPESVGKFGGDTDNWMWPRHTGDFSLLRVY
                     AGPDNKPAAYAPENQPYKPRHFLPVSLDGVKENDFTMVMGYPGRTDRYLTSYGVQMAL
                     DLTNPAVVKIRDKKLKLMKEDMDASTEIRIKYASTYAQTANYWKYFIGQSQQLKNNGV
                     VEKKKDLENKFQAWAGQDPQRLAKYGYALRDLETAYNDMRKLVLARTYLNEAILQGAG
                     VPFFAYQMGQLKEVIADTSNPAGLAEAIAEVRNEGKTFYKDYNISTDKKLLEAMLQMY
                     SEGIAKENQPAIFDSIRVRYNGNYKSFVDDVYARSVFATSDKFNNFLDNPNAAVLEKD
                     PAVELSNSFLKAFMTNFRPQITEVQSRIDKGNRAFVAGLREMMPEKKFYPNANSTMRL
                     TYGQVKDYDPRDAVEYDEFTTLKGVIEKEDPTNPEFHVPAGLKELYKKKDYGIYAENG
                     EIHTCFISTNDITGGNSGSPVINGKGQLIGCAFDGNWEAMSGDIFFEDQVQRTISADI
                     RYILFLIDKLGGAGNIVDEMQLMRKGKIVKR"
     gene            22838..23470
                     /locus_tag="PK281_01745"
     CDS             22838..23470
                     /locus_tag="PK281_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOY48091.1"
                     /translation="MKRVICIFVLLLPFLGFSQVGKLGRVSLGVRSTLSLFNHGQVKD
                     AGYGMGGHFRVQLTKRVNTEWFADYLTNNSGNTGFRQDGHIGWSVMYYLLDPNDFQRK
                     FTPYMVAGHCFDYSKLTVRPDGSSNILVALPQTGTRWTSAVQTGLGMHYNFTPKFDVS
                     FAAQYMFHFGHDLEIEEVPYRQVVIEDHDQHGIEGHLLVSLSANIKLVKE"
     gene            23467..>23684
                     /locus_tag="PK281_01750"
     CDS             23467..>23684
                     /locus_tag="PK281_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48092.1"
                     /translation="MKTRLILFSILLFGLHINAQEKIFTKTNGFRAMGTLCPGVFTSN
                     GATSLYFSGDWDYYFNEHFSMNGEGHYFL"
     assembly_gap    23685..23784
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(23899..26226)
                     /locus_tag="PK281_01755"
     CDS             complement(23899..26226)
                     /locus_tag="PK281_01755"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY48093.1"
                     /translation="MRLAFAIISFMMVFASSTLSSQNFQWVNWSPRAGVSSDFENFNK
                     VAVDPDGNSFMAVQFTGNLTIGNYSFESNFVEDICILKYAPNGDLIWAKAIGSQYWDQ
                     VNGMDCDEQGNLYITGHYFGVLWYENDSLVGNAGGREMFLMKILADGQVAWAVNGANV
                     WDDDGRDVRAMPGGGVIMCGRAHDTGHIGNLQLNNPNLLFQEFIAYFDDNGVGQWIRG
                     TNPSVVTYSNSKLEIGSDSSIVIAYSSGGPLEVNGDTLQPWYYPEFSANQDLIVQKFS
                     RTGDPIWGWIGGSLGQDLYGGLTIDNLGRVYFTLNSPMTSWFGQDSLSVIPGYWSSTV
                     YRLNSDGSEDTAWHFHSSGFSSLQDLTCDSEGNIWVGGIMRDTLYTSFATVYTPNVNS
                     REAMLYRINSTTNEVDRFDKIYGTGWYSILDLDFNEVTSSILFGGNGTASNAAPGTFG
                     LGEDLIIGFSSSPGNWAYIGSFKADSCENIAPLTVSDTLLCPGQTATLSSQQDFYYPS
                     WSNGSNEAQIIINSAAEIQYQAIDSSGCLVNLSATVESGIPVQFTATTTNLSCFGSAD
                     GAIDASLTSGMEPVNYSWSNGQTTQDLTNLAAGSYSLTAISAQGCSKNQSFTISQPQI
                     INAVLIESNGSLTLTSVTGGTPPYSYAWENLPDETGPSINFSSPGSYSVTITDSHGCS
                     ATKTLVATSTDDIANRHFALFPNPTSDALKIVHESGANFTYRIYSIDGRLVLTGNSIH
                     GNTIQTNDWKQGIYLIQIEESGSQPVIKRFEIIHP"
     gene            complement(26333..>26843)
                     /locus_tag="PK281_01760"
     CDS             complement(26333..>26843)
                     /locus_tag="PK281_01760"
                     /inference="COORDINATES: protein motif:HMM:NF015909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polyphosphate kinase"
                     /protein_id="HOY48094.1"
                     /translation="FKAPTEEEKSHDFLWRIHPHAPEKGMIQLFNRSHYEDVLVPRVH
                     EWIDKEEVNRRFGHINAWEQLLVDSGTLVFKFYLHISEEEQKERFEERINNPEKRWKY
                     KASDLEEAKLWPAYRTAFEDIFENCQKAASWQIIPADQNWYKDYLIASTIVEKLEKLK
                     MQYPQILDK"
BASE COUNT         7984 a         5372 c         5437 g         7950 t
ORIGIN      
        1 agcattgagt gtaaaactcg aactcgcttg aggctgagaa aaacctggaa tgattaaccc
       61 atcttcacct cgacaatcaa ccatgcgata agaaatgcta tcaccatcag gatcaaatgc
      121 tcctggatta tgtataaaga tttgtccggc acatgcttca tcaatgggtg gattgagcaa
      181 ttgcaccgaa ttattcacgc ccaagaaagg gttgataacc aattgggtag aaacgtaaaa
      241 cggaatatta accgaattgg ggatattcac taaaccacca ttccggttgg gatcttcgaa
      301 gctgagtgtg taaatagatg gtcccggata cgtgtgaaca cctacgtaaa tatttttctt
      361 gattgaagga gcaacaagtt cgcccattcc tgaaccattt atacgaggaa tggtgtcgac
      421 agaaccatcg ccccagctaa ttcctaaatc gggacgatcg gctggactgt cggatttggt
      481 gtaggtgatg atggtagctt cgtactgaaa tccacccaaa tgacgatagg taatttcgcc
      541 cgctctgtta tgcgtagcct gaagcacact acttaaaagt agtccgaaaa gcaaaagtaa
      601 gagccgaccg gtacgcatgt aaatcaggat tggtggcaga ggttctgcac cgaagcgaaa
      661 gatacgtatt caaacgaatg gcgcttctat ttgttgaaaa gcaaaaacga ggaccgaagc
      721 ccccgttttt cttaatttcc gaattgggat ataaacttga gtcgcattaa tcgaagctcc
      781 gtttcgccaa tttcgtcatc tcccatttcc tgaatcgcaa cagccatgga atcggtttct
      841 gcttttcgga aataatccat gatttcgtct tggatgtctt catcaattac atcatcaata
      901 tagtagctga tgtcgagacg tgttccagaa taaacaatcg cttccatttc ttcgatcaac
      961 gaaacgagcg tcaatccttt agaagaagca atgtcttcga gtgaaatttt acggtcaaca
     1021 ctttgaataa tgaaaacctt ttgagtggac ttgttgacca cgcttttcac caccatgttg
     1081 agtggacgat caatctcgtt ttcctgaacg tattgagcga tggtttcgac aaattctttt
     1141 ccgtatttct ccgcttttcc agctccaaca cccgcaattt gcttcaattc atccagcgta
     1201 acaggatatt gaatggtcat atcaaccaac gattgttctt ggaagattac gtatggcggc
     1261 atgttcatgc gcttagcaat cttacgagtc aaatctttca gcatcgagaa aagaacttca
     1321 tcggcagctg cggcaccttg accggctgct gctaattcat cttcatcgtc agtatcgctg
     1381 tagtcatgat cttcggtgat cattactggt tgaggattct ctaggaattc tgttccaaca
     1441 gaagtgagat gaagcgttcc gtagttttca atttcctttt ccaaaagacc catcacaaca
     1501 gcctggcgga taacaccact ccaatagaga gtagaatgtt cggagccttt tccaaacaat
     1561 tccaattgat cgtgtttgta ggaagtaatt accgagtcga ttgttccggt aaggatgtga
     1621 ttgatgtgtt tgtctttgaa ttttcctttt agggaagcta cagtatccaa cacgaggcaa
     1681 acatcttcca tcgcgtcgaa ttttttcttc ggatggcggc agttgtcgca catgttgttg
     1741 caaccgcttt cgtcgtattc ttctccaaaa taatggagta aatatttacg acgacacatg
     1801 gaagtttcgg cataaccaac cgtttcggct agtaattgct gaccgatttc ctgttcggca
     1861 acgtgtttat tttttaggaa tttctcgagt ttttgaatgt cgtcgaagct gtagaacgaa
     1921 agacaaacac cttcaccgcc atcacgaccc gcgcgaccag tttcttggta atatccttcg
     1981 aggcttttcg gcatttcgtg gtgaatcaca aaacgcacat cgggcttgtc gatacccata
     2041 ccgaaagcga tagtagctac aatcacatcc gcttcttcca tgaggaattt gtcctgatga
     2101 gaagctcgtg tagaagcatc taatccagca tggtaaggaa gagctttgat gccgtttaca
     2161 ttgagagttt cggcaatttc ttctactttt ttccggctaa ggcagtagat aattcctgat
     2221 ttgccgggat gttgcttgat gaatttgatg atttccttta ctacattttt cttcggacga
     2281 acttcatagt ataggttcga acggttgaag gagcttttga acaaggttgt ttctaccatg
     2341 ttcaagttct tcataatgtc ttgctgaact ttcggtgtag cagtagcggt aagtgccata
     2401 attggaactg taccaattgc ttcaatgatt gaacgaaggc gacggtattc ggggcggaaa
     2461 tcatgtcccc attccgagat acaatgggct tcatcgatag cgaaaaagct gatattgatt
     2521 tcgcggaaga actgaacgtt ttcgtctttc gttaatgact caggagctac ataaagtagt
     2581 ttggtcactc cgttagtgat gtcttcacga acacgggtga tttcggcttt attaagagat
     2641 gagttgagga agtgtgcaat tcctgttgca ccaccaaaat ttcgaattga atcgacctga
     2701 ttcttcatca aagcaattag gggagaaaca atgatggctg ttccttcgga aatcaaagcc
     2761 ggcaattggt agcacatcga cttaccaccg cctgtaggca tgataacaaa tgtatcgttg
     2821 ccatcgagaa tattccgaat gatggcctcc tgctcaccct tgaatttaga aaatccaaaa
     2881 tactttttta aatgacctgt aaggtctatc gtcgtatcga cagcttccaa catgctgttt
     2941 atcaataatt attaaacaaa tcgcaatttc cgaccactct taacaatgct ttttgtgtat
     3001 tgttgtcaat ccggaaaatt atcgggtatt tcatttgcat aaacgcaaaa tcatccttaa
     3061 agatacactt attaatcgtt ttgcaaagaa aatcattgct ttccatagta aaattttaat
     3121 ccgttgataa tcagatggat aaactgctga ccattatcct ctcaaacaaa acgattgaaa
     3181 atttgaaaag tgaagcgtca tttttagctg ctgcttgtat gctaactgat tttgacacct
     3241 tctaaaatcg gaataaaatt cggtttaatg ctgcggattt tggctttgtc aaaattaaat
     3301 ttggctaaaa atccccgaat ttgctcgaaa taaaccgatt ataggtcttg catcaacgat
     3361 tcagaatacg gatgtttgac aaaaaaaacg atgtcacagg aattttccag cgaacaagcg
     3421 gctgaattgg accaacaaga tgaattgaaa atcttccgtt ctcgattcag aatccctaca
     3481 ttacagggaa aagaagctat ttatctctgt ggaaattccc ttggtttaca acctaaagct
     3541 gttgccgatt ccattcaagt cgaattagac gattgggcgc agttaggtgt agaagggcat
     3601 gttcatgggc gtagaccttg gttaagttac catgagcagt ttgcagcacc tttagctcgt
     3661 ttagtcggtg cacgtgaaac cgaagtagtt gcgatgaatg gacttactgt aaaccttcat
     3721 ttgttgatgg tttcctttta tcgtccaagc agctcacgtt atcgaattat atgtgaggaa
     3781 aaggcatttc catccgacag ttacgcatta aactctcagg cggccttaca tggccttaaa
     3841 cccgaagacg taatcagaga aatctctcca cgttcaggac aagctcatat agaactatcg
     3901 gatgtacttc aagccattgc tgaagaaagt gaccggttag cgatggttat gattggtggg
     3961 gtaaactatt atagcggtca agtgttcgat atggcagcta ttacgcaagc tggacatgca
     4021 gttggagcaa tggtaggatt tgatttggca catgcgatag gaaacatcga attgaacttg
     4081 cacgattggg aggttgattt cgctgcttgg tgcagttata aatacctgaa ttctggaccg
     4141 ggaagtgtgg cgggagttta cattcatgaa aaacacgcat ctaatcctaa tacgtttcga
     4201 ttagctggtt ggtggggaca tgataaagca tcccgtttta aaatggaacc tgggtttaat
     4261 ccaattccaa ctgccgaaag ctggcaaatg agcaatgctc ccgtgctttc gatggctgca
     4321 cacaaagctt cgttggattt atttgatgaa gcaggaatgc cggcattaat tgctaaaagc
     4381 aaaaagctca cttcctattt acataaatta ctgcttgaag tgcaacaatt acctggtttt
     4441 gaagggatgt ttagtattat tacacccgat gaacgaggct gtcaactttc gcttttattc
     4501 catcgcgagg ggaagtcagt atttgatcac ttatttgccc atggcatcat agccgattgg
     4561 cgcgaaccta atgtcattcg aattgcaccc gtgccgcttt acaacagctt taccgatgct
     4621 tttcagtttt caaaaatctt atccatctac caatcaaact aagcagtatg tcgaagccta
     4681 aaaaaatcac gatcgctgga tcaggactcg ttggcgcatt acacgcaatc tatatgaaac
     4741 gtcaaggatt tgatgtggat ttgtatgaac gccgtcccga tttgcgaaac aaccgtatta
     4801 gtgcagggaa atcaatcaat ctagcacttt cagaccgtgg atttagaggc ttggcaggag
     4861 ttgggattga agatgatatt cgttcgataa gtattccaat gtatcgtcgc gtaatgcacg
     4921 atactaaagg caatcttact tttcaacctt atgggaaaga tgggcaagcg atttattcgg
     4981 tttcgcgtgg cggattgaat tgtatgttga tggatttagc tgaagcttct ggtgtgaaaa
     5041 tacacttcaa ctataattta ttgagtgtag atttaaattc agcacgcaca acgtttgtgg
     5101 gagaagatgg aatggtgaaa atagatgccg actttttagt tggagctgat ggcgcttatt
     5161 cggaagcaag aaacgaatta gaaaaaacac cttggttcaa ctattctcaa tactacattg
     5221 aatacgcgta caaggaatta agtattcacc cgaataatga tggttcgcat aaactggaga
     5281 aaaacgcgct gcatatatgg ccgcgtaaag acttcatgct tatagcttta cctaatttgg
     5341 atggaagttt cacactcaca cttttctatc ctaaaacagg agaacgttcg ttcgattctt
     5401 tggatacaat cgaaaaagca gaagcatttt tccgggagaa tttctcagat gccttggatt
     5461 taatgccaca ttttagagag gaatatgcat ccaatccggc tgctgcgatg gttattgtac
     5521 gctgtttccc atggagctgg aaagataaaa tgatgcttat tggtgatgct gcccatgcga
     5581 ttgtaccgtt tttcggacaa gggatgaatt gcgggtttga agactgtttc gtgtttaacg
     5641 aattactcaa tgagcatgaa ggtgagtggg gcgatttgtt acgtaaatac gaaattgctc
     5701 gcaagcccaa tggagatgca atagccgaat tggcactcaa caattttatc gaaatgcggg
     5761 atcgggtttc ggatcctaaa ttcttgttgc agaagaagat tgaagctcgt tttagcgata
     5821 aatatccaac taagtggatg cctttatatt cgatggtaac cttcagtcat attccatata
     5881 gtgaagcttt agctttaggt aaaaagcaag aagcaattat ggagaaagta atgaagctat
     5941 cggatattga atcgaaatgg gattcggaag aagtggagaa attgatgtta gaacacttga
     6001 actaagccgc ttctttttgc gatttagctt gtagttcttc ataatcatcc gcattcataa
     6061 tacgtgaagc tgcccagatg ctaatcacgc aagctacaat agcgatatag cccacatatg
     6121 ggtaatgttg tagtttgcct gtggcgtctt cggttacaat tgcgccagca ataaaagcag
     6181 caatacccga tgatgtttgc tgtaccgagg agttaaaact catgaaactt cctcgattct
     6241 tgggttgtgt agttgaggta attagggcca ttgctggcac aacgcgacca ctaatcacga
     6301 taaagaagaa acttgtaacg atcaatgcca tccaaaccgg aacaactggc atattcgtga
     6361 ttaagaaaag aggaataatt gccaaagccg cgaaaatcgc aaacaccttg tacttaccaa
     6421 ctttatcggc aagtttacca atcataggtg ctgcaaacac tgaacagagt ccgccgaata
     6481 ggtagatgta ggtgacttcg atttgtgtaa atcctacgtt tttctccata aatgctgcga
     6541 tgaatggtat aatggagaat tgtgataaca tcaacaaaga catcaacagt aaagctttac
     6601 gttgattgct attattgaac aaattgttaa tgactgtttt aaatcctggt cgggtggatt
     6661 tatcttgtat atgtgtgttg atggaaggaa tgaaacggaa tatgaaaaag gcaacgacta
     6721 atccaacagc agcaatgaat agaaaagggg cgtgccagct atagatacta gccaagtagt
     6781 ttccgaatgg tacaccaaaa acggatgcaa gtgaaaatcc ggcagttacc attcccatag
     6841 cagaagctct tcgttcattg ggaatgacat cacccacaat agataatatt aaagtgctaa
     6901 gcaaacctcc aaaagctcca gtaaagatgc gagcggcaag cataaacgca tagttaggcg
     6961 caaaagcaca ggcgagtgtt cccaccaaaa agcctgtata acaaacgaga aggattttct
     7021 tccgatcgaa attatcaacg aagaaagcac cagcaaatcc aacaatacct gctgttatgg
     7081 tatatgccga tacgataaat ccaaattcct gcggctcaat attgaacaag cgcatgagtt
     7141 gatttcctaa aggcatgatg atcataaaat ccatcacatg cgtaaactgg ataaatgcca
     7201 gtgttaaaag gatactatac tctttacgac tcatgaaatg gatgggggaa tcaaacgaaa
     7261 ttgctcgtcc gaatggattg ggattttcgg agtgcaaaga aagtagaaac cagacaatcg
     7321 gaatgttaaa atgttctgag tttataagat tcggtataat ccaaaaagcc gcctcctatt
     7381 aaagaagcgg ccttgagcac acacaatgaa ttccttagtt cttgataaaa cgagttgtgc
     7441 tagttttatc ggctgatgtc attcgtatta aatagatacc agcatttaga ttaccaagat
     7501 taatttggtg gttttggtta gggttaattt ttaggttttc ggtttgaact attcggccgc
     7561 ttaggtcgat gatttccagc tttgcctgtt tcgcttgttg agatttagcc caaacaatac
     7621 tcaggttttc ttgagcaggt gatgggaaaa gggaaaagtc aacggagttt gcagggtttt
     7681 caattgattg gcaatcaact actacaatgc taatggaaga agatgtaagt gtatttactg
     7741 catcagtagc agaaagtgaa taggttcccg attctgttgc agttaaagtt gtatcgttaa
     7801 gaacgccgat atctactcca tcttttttcc attgataggt aaagcattca cttggttgga
     7861 taatagttaa aagtgtttga tttcctacac aaattgtgtc tggaccaaca ttttggattt
     7921 gcagaatctc ctcgatgagt ttgatgcgag ttatacctac gtagccaatt gataaagtcc
     7981 caacaactaa ttcatcatcg ttttcaaaat aagcgcatct gaaagttgtg ccgtttaact
     8041 ctgtgaaagc cgcatatccc gttccttcga aacttgtgtc atagtttcct tgatcattca
     8101 aacgatagat gattcctttc gtttggccaa aagcaaaaga acctgaaccc atacaccaaa
     8161 accgattttg actatcctga tacataaaat gtccggcttc atcattattt gttcccggat
     8221 aatcgattaa atcatttatg ccatcggaag aataacttga atctggcgca ccagtttcgt
     8281 ctaatttgaa aatctgtaaa tcgcttgtta cgttggtatg agaattatac ccccaaatag
     8341 ttccattatc tcctgaaaaa cttcgattta aattaacatt ggaagctggt atattggtcg
     8401 tcagaacgcc atttgtacca tatgttggaa cgaattcacc taatgaatct agtttaatag
     8461 aaactattct agccggacta aaatctgatg cataaacaaa gcgaatagag ccatctgtat
     8521 cgtaaatacc gggtgagcta taaccattaa ttggtaatgg agtggatgga tatggataca
     8581 cgacgattgg atggaatgta ggatctacac taccatctgt taaataacgt cgtgcaatat
     8641 ttttcgtgtc accaccatta atatttcctg tttccatacc atacgcaacc actttaccat
     8701 cctcttgagc aaggagttgt gtataaaaag aggaagaaac agaattggta ctagttaatt
     8761 caactacatg cccatttcca tggaatgtag aatcaggatt gccattggaa ttgaaacgag
     8821 ccacataagc tctaattgaa ctatagccat tatcaggagc ttcatttccg catacataat
     8881 atttgtcacc ctgcttcaaa atgcaattca ctgtattacg ctgactgaaa acccatctct
     8941 tcaccccatt taaaccgaat gtgctgtcaa tactaccatc gggattaaac tgcatgaaga
     9001 aaatgttata tgaatttgca gcaccatcaa caagatcacc tacaatgagg tatttatttt
     9061 cgtttgtttt gaaaatctct cttggtgagt aattaacaac atcatcaaac gtggtaagcc
     9121 cacaggtagt cccaaatgtt ttatcaatta ggtaatgctg ttgtgcttgg gatttagtaa
     9181 ttgagaacag acaagtcagt aaaatgaaaa agaaggtaga tttattcata gtgaattaat
     9241 tgacaatcaa ttttttggct tgtttgttta atagtcgaat tggaatttga tatctcaatt
     9301 gactgatagc aaagcaaaac cagatcgaaa aacatcaatc aaattaacag gaatagaaga
     9361 aacgtgtaaa tagctaatac tagctagcag atcccaatct taattataca agtcgctctt
     9421 tcaaggcttt ggctactgct tcacccatcg aatgcacgtg cagtttgtcg taaatattgc
     9481 gaatatgtgc atttacagta tggtaactta aatcggcttt ggcggctatc attttataac
     9541 taagtccatc tactaataat gctagaattt cgatttcgcg aggagtaagc gtgtaatcat
     9601 tttcaggagt gttttttacc tgttgtttga agaacatcaa tacgcgttgt gcgatagctg
     9661 gtgtcatggg tgaacctcca gcaacggcat cacgaatggc ttcaatgatt ttatcgggag
     9721 aagctttttt aaggatataa ccacttgctc cagccatgat actttcgaaa atctgatcat
     9781 catcttccga aatagtttgc atgatgaccg ctaattcggg atgctttttt cggataatgg
     9841 aaacgccttg aatcccattc acattgggca tttcgatatc catcaaaact acgtcgggtt
     9901 gcgtttcatc tattacttca accactcggc tgcaatcttc aaacgcacca caaaactcta
     9961 gatcgggata aagagaaatt aatacacgta agctatcccg gcggggagcg ctgtcatcaa
    10021 aaatgattac ccgagttttt ttcattcgat aaaattacct gcattctata tacaaccaac
    10081 tcatttcgcc tccctaattc ttgctacagg taagtgaatg gtgatttttg ttcctaatcc
    10141 aatttcagat tcaatggcga tttcaccttg taattcagtc acacgatttc gcatgtttct
    10201 aaatccattt cctcctttgg tctcgacatg ttggatgcct ttcccattat cggaaaaaag
    10261 caaaatcaag gtgtcatttt taagttttac cgaaattttg agttcgctcg cttcgctata
    10321 tttcgctgca ttattgacga tttcttttga aatgaggaag atgtttttac gcgcattcat
    10381 tccaatatgc atatcgttgg ctagctcatt aacataatga acttccatgt cggaagcttg
    10441 gagaacttcc atacaatgca ccttaatacg attgaacagg ctctgaagcg tatcattatt
    10501 cgggttgata ctccaaacga tatcactcat ccgatccatc gtttcggatg catttttatt
    10561 gatttgattc agcaattcgt tcactgtttc gggagaagcc tgcatgcgct ccattgctac
    10621 tttactataa atgttgatac tcgataaaga tgcacctaca tcatcgtgca aatcactact
    10681 tatcgagttt ctcacgtgtt gcatcttttt taactgattc aatcgatacc aataaatccc
    10741 atatacaatt cctgccacta taagtaatgc caacacgcgg aaccaccagg ttttccaata
    10801 cggaggagct atcgttattt tcaaatgtgt aacatccgac caagcacttc catcaactcg
    10861 agccttaatt tggaagtcgt attctcctcc ttgtaaatcg gtgtaatcag catagttttt
    10921 cgattgagaa ataatccaat catcattcac tccttttagt ttactcgcat attcgattcg
    10981 atctttatac aagaatggaa aggatgaaaa ctccagcgtg aagaaatttt gcttgtaatt
    11041 aaatgcaagt tctccagttg atggaatgcc aatttcttta tccaataacc taaaagatgt
    11101 aattactggt ttttggacca attgattcaa atactgcgac ttaaatccat aaaagaacgt
    11161 tttccaaaag aaccaaattc gcccatctgg acctttgtat cctgaactcg aaaacaaagg
    11221 aacatcttgc ataaaatctt ggttgacagc tttcacaaaa aggtcgcttt ttctcaaggt
    11281 tcctattcca ttgttagttg tgaaaagcaa attccccttt cctacatcta gtattcgata
    11341 cacataatca cctatgagtc cggtatgtcg acttaggtaa ttcatggttt tggttttgaa
    11401 atcatagata cacatcccgc ctccgtgtgt tccaatgtat aaattgacgg aatctgcata
    11461 aagggcatcc accacaccat taaagcgagg gaaacgttga gtagttaggt attccgaaaa
    11521 agtttttgag gattcgtccc atttccaaac acctgcaccg ccattaaagc ctacaaataa
    11581 atcctgactt gactcataaa tagagattaa tcctgcatta ggccagtttt tagatgactt
    11641 ttttgaagtg ctgtgataga aaactggacc atcccaagta gcaaatccaa ctaaatcccc
    11701 acgccaggtg ccagcccaaa gccgaccttt agaatcggta tgtacaaact gtaaaatatc
    11761 acgcttgtgg ccgaaagcag aagtgtcttt gaattctcgg aatttactta ctcgcaggtt
    11821 gtttcgatca atctgaatta aaccttcgga atgtcctgca agcaacatgc ttgtcccagg
    11881 aactgcatac aaacaactca acgatttagg ttcaatatcg ggtaatgaga gtttttgacg
    11941 gatttcagtt tttaaatcat tccgatagat cccatctgtg gcaatcagcc aaatcgcatc
    12001 tttatcagca accaatgatt ttatagacca atcatcgggt gtatcattga accaatgtaa
    12061 ttcgttgttt tgggttttag catttcgagc gaaaacacca ttactggttg ctatccactc
    12121 aatattcctc gaatcgtgta agatgaaatt agctttaaca ttaccgatgc taccaggata
    12181 ttctagtgta aatggttctg tataccgatt cctcttgggg atggaaatga aaaatggcgg
    12241 ttcatcccaa aagctaaaaa gcaaagcctt gtgatcttgg gaataatgca ttttatgagg
    12301 agttttttct ccaactaaac aggtaattcc ccaatcttca aattggtttt tttgaggatt
    12361 aaatgagcaa atatgatttg ttgactcatc aataatccaa atagtttttt cttcaccaat
    12421 tgcacctatt gaatgccatt cgcgagtaaa tacttcccaa tttttgggat tgtacatgga
    12481 ggaataaaac tttcccgtag atgcttcaaa ataagcaatt tgtcctcctt gtaagaacca
    12541 taatccttga tattgtggac cacctaaaag aaatgcctga aatccacttt gtttacgctt
    12601 tgctcctgga atggtaacta gtttaaatac cggtcgggag ggtgtataca agaacattcc
    12661 accttccgta acacacaata cgcttccgtc aggattgaca ataagctggt taatccgatt
    12721 ggatagggtt gttaagaatc gatcagattg caactgataa tgctcaaacg tttctttgat
    12781 gggatcaaaa actcctatac ctgaaccgta tgttccaatc cagatttttc catccggagc
    12841 ttctgccaaa catttgatgg tattgccaca gagagtagtt gtgtcggccg gattatgaaa
    12901 atactgctta acttgatgtc cgtcgaaacg atttaaacct tcagatgttc caatccagat
    12961 gtatcctttt gaatcttcta atagcgtatt gcaggtattg gatgaaagtc cgttgttaat
    13021 tgaaaatcgt tggtaaaact cactaacaga ttgactacga agattgttgg atatgaataa
    13081 tagccaaatg ctgactgcta ttttgaatag atggctgatt gcttggtttt taccaaggaa
    13141 gttattctgg atgaacttta aacttcctac caatccaacc attttagatt agctaagaat
    13201 ttgaaatgca ataaatgcag aaagccaagc tagaacaccc atgtaagtag tttgagcgat
    13261 ggcccatttc caacttcccg tttctcgaat aactactgca atggtactca tgcattgcat
    13321 ggcaaaggca taaaacaaga taagtgcaat agatgcagct ggactaaata aaggcaatcc
    13381 ggtgcgatcg ttcgtgtcgt tcatcatccg ttcacgaagt gttgcttcat cgtcgtcgct
    13441 accagcacca taaatggttg ccattgttcc aacaaaaact tctcgtgcag caaatgatgt
    13501 gaccaaggca atcccaattt tccaatcaaa accgagcgga gcgataacag gctcaatcgt
    13561 tcttcctaaa attcccgcat acgaattacg aagcttttcg gatgaaatcg cgttagcagc
    13621 ctgctcttca cccatttgtt caatcataac tgggctagtg tattttttct caatttgctc
    13681 gaaacgatca cccggtccgt aagatgaaag tgcccataga agtatcgaaa tggcgataat
    13741 cacctttccc gcatcgcgaa taaataccca aactttttca cccatggtat gtgctaccac
    13801 gccccattga ggcatgcggt aaacgggaag ttccatgata aagtagcttc gttcgcgtga
    13861 gcggataatc cattttaaaa tccaagctgt tccaagcgca gctaaaaacc caagtaagta
    13921 taagcccatc atgatgagcc cttttaggtt gaagataccg atgtgtttat caggaactgc
    13981 aatggctatt agcaaggtat aaaccggtaa acgagccgag caactcatga gtggagtaac
    14041 caaaatagta atgagtcgtt ctttccaatt cttaattgta cgagccgaca taatcgctgg
    14101 aaccgcacac gcgactccac tgataagcgg aattaccgac ttcccattaa taccgaattt
    14161 gcgcatgagt ctatccatga taaagctcac acgtgccatg tagcccgaat cttcgagcac
    14221 agcaataaag gcaaaaagaa tagcgatttg gggaatgaac atgagaattc cacctaaacc
    14281 tggaatgatt ccttgagtaa ttaaatcgtt gagttttcct gccggaagtg tttcacctat
    14341 ccatgatcca gacttctgaa acaagctatc gataagatcc ataggatatt cggcccagga
    14401 aaatatggct tgaaagagta aaaacaggat cgtgagaaaa gtgagaaagc cccaaactgg
    14461 atgggtaaaa acatcatcca atttcatcga aaatcttagt gctcgtgtaa tagtaacctg
    14521 agccagacaa ttatcgagta tggtttgaat cattccgtaa cgacgaagtg attcttcact
    14581 ttgcaatcca aatggagtga gttcgtcttc ggctaacagt tttcgtaaat ctggaagcgt
    14641 gcgttccgat tgcaaaaggt cgtagttggc agctgttaag tatgcttcgt aagcagaaga
    14701 aattcccgtg cttttctgta atttctccat cacacccgga tgataatcgg ccggattgtc
    14761 gaaagttttc atcgcgccaa cttgttcttg gaagatggct tgttttattt cggagattcc
    14821 ttttttatcc cgcgcattta caggaataac aggtattcct aactcagctg ctaaacgaac
    14881 ggtattgagt tttaagttac ttcgttcgag aatatcgatc atgttcaccg ccaaaatcgc
    14941 gggtaaacca aggtctatca cttgaagaca aagcaacaag gagcgtttca gattagatgc
    15001 atctgccaca attagcacca tgtctgggaa atcgggcgaa cgatggtcgg ttaggacctt
    15061 gaatgtttcg agctcgtcga gcgattttgg aaacaagcag taagttccag gtaaatcgat
    15121 aaagttaacg gtgcgacggc ttttgtcttt tggatgaaac atcctgacct gacctgtatg
    15181 tttttcaacc gtaacacctg gaaaattccc cgttttctga tgcaagcctg ttaacgcgtt
    15241 aaagagcgtt gatttcccac tattgggatt tcctaccagg gcaatatgaa tgggtttgtc
    15301 ttccacagga aattatgcgt gttcttcgac taaaacggtt gaagcttcta aaagccgaat
    15361 gctgataagt gaaccagaac aggcgaaagc gattggatca cccagaggag ctttgcgaat
    15421 catggtgata cgagtaccgg gaatgcaacc taattcgata agtttgagcg atatttcttc
    15481 gtcggtaaat ccacgaataa tagcggattc gccaatggga acatgagaaa gatcggccga
    15541 catgatagct tgtttagaat gattctaatt aagtgcgaaa gtacaaattt cgcgatgcaa
    15601 accccgaaat gagaaaaaag aaaatgtggc ttagcagaat gaaggattca agtgcttctg
    15661 caccatggca atacgcttct ttttgtgatg gtcaggacaa tcgcatttct tcttaaagag
    15721 tttgcaactg ggcgtaacta aaacagcaat caacacaaat agaggcagga gttttctcat
    15781 gatggaatta gaacgcgaaa aataaggaat cgtttcagcg ttgcaaaact ttaaaggcaa
    15841 tatcgcgtcc tagaccagat acaaaagcca attttcccca aatcatcgca agattttcta
    15901 aaggtaattc catctctgga ccgcctacgt caaacggtcg aaaacctgcg tcagttgcta
    15961 attgtagcac agtttgctta tcctccggat aacgtccaca aataaataaa tcggccactt
    16021 ggtcaccata tttgggatta tccatgtttt cggcaccaat catattaaac gccttcacaa
    16081 tatgatcgtt tccacaagca gccaaaatgg cttcggaggt gcgataaaaa ccatctggct
    16141 ttcggaaaac agcattcatc gcatcaatca ctaatttgcc atctatattt cctaattgct
    16201 tcaccacatc ataagttgca tctgctggta tagctaacaa aataatatct gctttggatg
    16261 ctgccacttc aggtgcatga gccgaaatca agtccggatt taattgtacc aaactgcgaa
    16321 tcgaatcatt caaaggattt cgagttccta aataaattcg atgtccggca tgtgcccaaa
    16381 ctttagctaa gcgaccgcca atatttcctg taccaataac agctatattc atatctagta
    16441 gaacgatttt actaaacgaa tgtataaaaa tagaggcttt agcatcagct ttattagctt
    16501 tgtgccatgc gttatgtatt gtcttttgtg attctgtgca tttctggatt tgtttccctt
    16561 gcacaaaccg ataatatggg tgttggaact ttaactccag acccaagtgc caaattagaa
    16621 gtaagctcca ccagtaaagg attcttagct cctcgcatga gttcagttga gcgattggca
    16681 atcctcaatc cggctcaagg tttacttgta ttcgacctaa ctaccaattc gttctggtac
    16741 tttaattctg gtacttggac cgaactcatc acacaaattc cagctgcacc atctgctatt
    16801 ttattcagct cgtttagtca aaccggaatt gccggaagcg cagaagcaat catgggttca
    16861 tacgtgattc cagctaatac gcttgatgca aacggacaag ctattctggt tcatgctttt
    16921 ggtttactca cgaccgatac agcgcttgtt cgcttaaaat tttcaggcat tgagttacct
    16981 tttcaaacat acggacaagg tttttgggaa cttacctgcc gggtttatcg aacatcagcc
    17041 aacacctcga aatgtgcggg aacattggtt gtgaatggaa gttcaagcac ctttgtttct
    17101 caaccagcaa tggattttag tgcagccatt ccattccaaa ttactggaca acaagttcag
    17161 ccagtagtaa atggagtgag tctggaagga tttgaaatta ctcgaatccg atagttattt
    17221 cccggctagc acatcttcca cccaaccttt tccccattct tcgatctcag cctgcgacca
    17281 taattcggga tagaaaatcc gtttttggaa acgcggagga agatatttct gccagtttgt
    17341 accgcccgtt gctttaatgt cttcagggtc acgttgcaga taacgaacag cgcttttgta
    17401 atgtgaaagc ggccagttga tgttgactaa aatgtcgaac gcgcgcataa cctgtttcaa
    17461 ttcacttaaa tctgctccac ttctttcaag cttacgataa acagcataaa tgttcgattg
    17521 cacattgctt tcggctaact cgataagttg cttgctgtat ttcttctcga attgttcaag
    17581 cgtgagtgtt ttctttcctg tagccaattc ggtagctcca gcttgccaat agatatactt
    17641 gaattgttcg gtaatgcttg cttgagttcc ttcaaatttg gaacgatggt ctttatcaac
    17701 caaatggata aaatcggtgg caaaaatctc taccattcga tattgcgcac tttgaaaacc
    17761 tgacgctggc aaaagcgaca tgcggaattg cagaaactgc tcacgatcca ttccatccac
    17821 cattacttca aacgaatggg ttagattcat gaaataacgg ttcacacgtt tcagacggtt
    17881 gatggtgaac tttaagtctg gttccgattg agcacctatt tgcttcaatt catgtaggct
    17941 gagtttgaaa tacaactcag ttatctgatg atacatgatg aagatttctt cgtcgggaaa
    18001 actagtacgc ggattttgaa gactcaacaa ggtatcgaga tgaatataat cccaatatgt
    18061 gagaaaatcg gcgtgtagca agccgtcgag ataggaacgt aaatcctgtc ccatggctgc
    18121 atatttcttc tgcaaaagat cgatgcggga aaggatgtct tcggagattt ccattcttta
    18181 gcgtgattga ggtagggcaa acataggtaa aaaggcagaa tagaatgagt ctttcgaggt
    18241 attctattcg aatgttgggc gagaaaactg tccttatttt ttctttaaga agcaatcttt
    18301 cacatgatcg tttaccatgc cagcagcttg catataagcg taacaaatgg tagtgccaac
    18361 aaaggtaaaa ccgcgttttt tcaaagcttt gctcatttca tcgctttcgg gtgttgaagc
    18421 tggaatttcg ctcgtcgatt tacgtttccc atcgagcgtt ttgcctcccg taaattgcca
    18481 gatataggca tcgaatgttc catattcttt ctggatttca agaaacgcac gcgcattttt
    18541 gattgtaccg gtaattttca aacgatttcg gatgatgcct tcatcttcca tcaacatagc
    18601 cacatgatca tccgtgaact tagccacttt tttaggatca aatccagcaa atgcattcct
    18661 aaaaccagct ctgcgattga gaatggtttg ccaacttaag ccagcctgaa agctatctaa
    18721 tactatgaat tcgaaatgtt tacggtcgtc atgaaccgga attccccatt cggtatcgtg
    18781 atattcgcgc atgttatcag tcttggtaca ccaagaacat cgaataaggt ccttcatttc
    18841 tctcaaaatt taatggctga aaataccaat aatgacatac actttgagtt ctatatttta
    18901 atctttcccc gaaatgttac tattcaagca taacctatct tcgcagtaaa ttctaaaccc
    18961 atgctccgaa cgctcatcgg ggtttttgtg cttgtcggaa atgctcttct tgcgcaaacg
    19021 tcctcgctta tttcccaaga tgctttattt cagattcccg gtagagaatt acctgaaagt
    19081 agcgatttgt ctgctaaagt tcgtggctcc atcagtcccg actcaatttt ctttgatgtt
    19141 gaggtaaccg atgatcaaat acaagccaat acagcaggaa cgggcgatcg aattgaaatt
    19201 tggtttggtt ctccttgggt gaattttagt gattacatca tcggggaaaa aggaaaaaaa
    19261 accttcattt tccgcaatac tgccgaggct ggtgatgatg ctaatttgga acgttttatt
    19321 cgcgatgctg attatccaaa ggggaaattg aaagatgcag aaaccggaaa agatgttaaa
    19381 cctgttgtgc ccgatgctaa tagtttgcgc cgtgaatatg tgttttttgg catgacacga
    19441 tttggcttat cagcttctca acagcctgct aaacacctcg atcgcgacaa gtatggtcca
    19501 ttcgaaaagc aaacgggttt gacactcgat gatttatcac cctttgtgaa ttataccgtt
    19561 acgaaaaccg caactggact gcattatcat gtagcgatgc ataacaagtg tttaggattt
    19621 ggacgagttg cttcgatgaa taaaattcgg tttgtggttg atgttattga tgctgatccg
    19681 ggagaagtta aggagcaagt catcagtaca acttcgaatc gtttttatgg tcgaccatat
    19741 tactttaatc aaacagaatt agcatttcca ttaaacattt taccaagcta tgttccggct
    19801 catattctcg aacgcatgca aataaatctg gatgtgattt atagtggagg aacctggaaa
    19861 acatttggtt ggaacagtgg aggaatcgtg tatgcgaaag aatttgtctc tgaagctctt
    19921 accgaattct atttttatac aattgatatt tcttaccaaa aaattcctga aggacaacaa
    19981 gttgaacatg agcgagttga acttaaatac gatgatgtga gtattttccc tcaacatgaa
    20041 gtatatctat tacttaacaa tgaaatttat tcggggaaag actatcgcta tactggtcat
    20101 ttagataata atttctttaa cacgattttt cgcttgcctg atggaagcac agcaatggta
    20161 ctttatgatt acgaagtagt tgacccgctt ggatttggtc agtttggcca tactgctgat
    20221 gagttcattt ttatcgaacg agttgtgggc aaagaaatga gtcctttaat caatattggt
    20281 caacgaatcg aagctgcagc aactttagtt gtgggagaaa acagccaatt taaagttact
    20341 gatgtaaagt ctgtacacta cgcctgggtt gaatttggga agttatttga agttcgaatc
    20401 aagggcaaaa cctccgagtc atctcgaact ttacgttatc gtatcaacga agaaggaaaa
    20461 attgttgact ctaaatgatt ttgctccacc cgtaggtggt tgtttgttgc gagtattctc
    20521 gcgttatttg cccctcacaa aattaacctg tacaacctca atgaaacgtt tcttaattat
    20581 ctgccttgta cttgcaggaa tgacgtcgca gcaggtagtt cgtgccgatg aaggcatgtg
    20641 gcttccattg cttttaaagc gtttgaacta cgaagacatg aagaaaaaag gctgcaaact
    20701 aacagccgac gagatttata acatcaatac atcaagccta aaagatgctg tggttatgct
    20761 tagcggtgga tcttgttctg ctgaagccat ttcatctaaa ggtttattgt tgactaatca
    20821 ccactgtgga tacgaagcta tccaaacgaa ttcaagcgtg gagcatgatt acttgacaga
    20881 tggattttat gctaaatcgc tttcagaaga acttccttct ggtggcatta ctgcatcatt
    20941 cctaattcgt attgaagatg tttctcaacg tatcctggcg gaattaaatc cagctatgag
    21001 cgaaagtgag cgtactgcaa aagttcgtca gctttacaaa acaatcgaga aagaagctac
    21061 agaaggaact ggctacgatg ctgctgttaa aagctttttc gacggaaacg agtattactt
    21121 attcgtgtat gaaacattca ccgatgttcg tctagttggt gctcctccag aatcggtagg
    21181 gaaattcggt ggcgatacag acaattggat gtggcctcgt catactggag atttcagctt
    21241 actacgagtt tatgctggtc cagataacaa acctgctgct tatgcgccag aaaatcaacc
    21301 atacaaacca cgtcactttt tgccagtttc gttggatggt gtgaaagaaa acgatttcac
    21361 catggtgatg ggatatcctg gaagaaccga tcgttattta acttcttatg gagttcaaat
    21421 ggcgcttgat ttaaccaatc ctgcagtcgt aaaaatccgc gataagaaat tgaaattgat
    21481 gaaggaagat atggatgcga gcaccgaaat ccgtatcaag tatgcttcaa cctatgctca
    21541 aacagctaat tactggaagt actttattgg acaaagccag caattgaaga acaatggagt
    21601 agtagaaaag aagaaagacc tagagaacaa attccaagct tgggctggac aagaccctca
    21661 acgtttagct aagtatggtt atgcattgcg cgatttagaa acagcttaca acgatatgcg
    21721 taagttagtt ttagctcgta catacttgaa cgaagctatt cttcaaggtg ctggtgttcc
    21781 attttttgct tatcaaatgg gacaattgaa agaagttatt gctgatacat ctaatccagc
    21841 tggtttagct gaagcaattg ctgaagttcg taacgagggt aaaacgttct acaaagacta
    21901 taacattagc accgacaaga aattgttaga agctatgtta cagatgtatt ccgaaggaat
    21961 tgctaaagaa aaccaacctg caatcttcga ttctattcgt gtacgttata atggtaatta
    22021 caagtcattt gtcgatgatg tgtatgctcg ttcagtattc gcaacgtccg ataaattcaa
    22081 caatttccta gacaatccta atgctgcggt attagagaaa gatcctgcag ttgaattaag
    22141 taatagcttc ttgaaagctt tcatgacgaa tttccgtccg caaatcaccg aagttcaatc
    22201 tcgtattgat aaaggaaatc gtgcttttgt agctggttta cgcgagatga tgccagagaa
    22261 aaaattctat ccgaatgcta attcaactat gcgtttgact tacggtcaag taaaagatta
    22321 cgatccacgc gatgcagttg agtatgatga gtttactaca ctaaaaggag taatcgagaa
    22381 agaagatcca acaaatcctg aattccatgt tcctgctgga ttgaaagaac tttacaagaa
    22441 aaaagattac ggtatttacg ctgaaaacgg agaaatccat acttgtttca tttctaccaa
    22501 cgacattact ggaggtaact ctggaagtcc ggttattaat ggaaagggac agcttattgg
    22561 ttgcgcattc gatggtaact gggaagcaat gagtggagat attttctttg aagatcaagt
    22621 acaacgtact atctcagctg acattcgtta tatccttttc ctaatcgata aattaggagg
    22681 agcgggtaac attgtagatg aaatgcaatt gatgcgaaaa ggcaaaattg taaaacgata
    22741 aaaattcact agtgagagaa cggaggttga ttattcagcc tccgtttttt ttatgcattt
    22801 ttttcagtca tttacgttac ctaatcaaac actacacatg aaacgagtaa tctgcatttt
    22861 cgttttattg ctcccttttt taggattctc tcaggttgga aaacttggtc gagtttcgct
    22921 gggagttcgt tcaactttga gtttattcaa tcacggacaa gtgaaagatg ccggttatgg
    22981 aatgggcggg cattttcggg ttcagctaac gaaacgtgtc aataccgaat ggtttgccga
    23041 ttacctaaca aacaattctg gaaatactgg ttttcgacaa gatggacata ttggttggtc
    23101 ggtgatgtat tacttgcttg atccgaacga ttttcagcga aagtttactc cctacatggt
    23161 tgcaggacac tgtttcgatt attcgaaatt aacggttcga cctgatggtt caagtaatat
    23221 tttggttgca ttaccacaaa cagggactcg ctggacttcg gctgttcaaa caggattggg
    23281 gatgcattat aatttcactc ccaagtttga tgtgtcgttt gctgcacaat acatgtttca
    23341 ctttggacat gatttagaaa tagaagaagt gccatatcgg caagttgtta ttgaagatca
    23401 tgaccaacat ggaattgaag ggcatttgtt agtttcctta agtgctaaca ttaaactcgt
    23461 gaaagaatga aaacgcggct aattctcttc tcgattctat tatttggttt gcatataaat
    23521 gctcaggaga aaatctttac caagacaaat ggatttagag ccatgggaac tttatgtccc
    23581 ggtgttttta cttcaaatgg cgctacctca ctttattttt cgggtgattg ggattactat
    23641 tttaacgagc atttttctat gaatggtgaa ggacattatt tcctnnnnnn nnnnnnnnnn
    23701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23761 nnnnnnnnnn nnnnnnnnnn nnnntttcac tcagcgaatt tcgttttatg ggcggcttag
    23821 gttggaattt aggggtgaag aacaagtaaa ttcttctctc caaattcgtt aagcatgttt
    23881 tcttttagaa attgatgctt atggatgaat aatttcaaac cgtttaatca ctggctgact
    23941 tcccgattct tcaatctgaa tcaagtaaat cccttgtttc caatcgttcg tttggatggt
    24001 atttccatgg attgaatttc cagttagcac taaacgtcca tcgatcgaat aaatacgata
    24061 agtgaaattc gcacccgatt catgtacgat tttcaatgca tctgaagttg gattcgggaa
    24121 taatgcaaag tgcctattcg caatatcatc tgtacttgta gcaactagag ttttggttgc
    24181 actacatcca tggctatcgg ttatggtaac agaataactt cccggagagc taaaatttat
    24241 tgaaggacct gtttcgtcag gtaagttttc ccatgcatac gaatacggtg gagttccacc
    24301 tgttaccgat gttagtgtaa gtgaaccatt cgattcgatc aacacagcat tgatgatttg
    24361 cggttgagaa atagtgaaac tttgattctt ggaacatcct tgagctgaaa tcgcagtaag
    24421 agaatagctt ccagcagcga gattcgtaag gtcttgggta gtttgtccat tcgaccatga
    24481 ataattgaca ggttccattc ccgatgttaa tgaggcatca atagctccat cagcagatcc
    24541 aaaacaactg agatttgtag ttgtagcagt gaattgaaca ggaataccag attcaacagt
    24601 tgcactaaga tttaccaaac aaccactcga atcaatagct tgatactgga tttcagcagc
    24661 actattgatt ataatttgag cctcgttgct accgttgctc cagcttggat agtaaaagtc
    24721 ttgttgacta cttaatgtgg cagtttgtcc gggacaaagt aaggtgtcgc tcactgttaa
    24781 aggagcaata ttttcgcagg aatctgcttt aaagctgcct atataagccc aatttccagg
    24841 ggaagaagaa aaaccgatta ttaaatcttc acctaatcca aaagttccag gtgctgcatt
    24901 acttgcagtt ccatttccac caaacaggat gctcgatgtg acttcgttaa aatccaaatc
    24961 taaaattgaa taccagccag ttccataaat tttatcgaaa cgatccacct cgtttgtagt
    25021 actatttatc cgatacaaca tggcttcacg actgttaacg ttaggtgtgt aaacagttgc
    25081 aaacgaagtg tataatgtgt cacgcatgat gccgccgacc caaatattac cttcagaatc
    25141 gcatgttaga tcttgtaagg acgagaaccc cgatgaatga aaatgccaag ccgtatcttc
    25201 agatccatcc gaatttaatc gataaacagt tgatgaccaa tatcctggaa taacggaaag
    25261 tgaatcttgc ccaaaccaag aagtcattgg tgaattcagt gtgaaataca ctcgcccgag
    25321 attatcgatc gttagtccac cataaagatc ctgacctaat gaaccaccaa tccagcccca
    25381 aattggatct ccagtacgag agaatttctg tactatcaaa tcttgattgg ccgaaaactc
    25441 ggggtaatac caaggttgaa gggtatctcc attcacttca agtggaccac cgcttgaata
    25501 agcaatcaca atagagctgt cggaaccaat ttcaagctta gaatttgaat aggtaacaac
    25561 gctagggttt gttcctctaa tccactgtcc aacgccatta tcgtcgaagt aagcgatgaa
    25621 ttcctgaaag agtaaattgg gattgttgag ctgcagattt ccaatatgtc ctgtatcgtg
    25681 agctctaccg cacattataa ctccaccacc aggcattgca cgaacatctc gaccatcatc
    25741 atcccaaaca ttggctccat tcacggccca agcaacttgt ccatcggcta aaatcttcat
    25801 caaaaacatt tctcttccac cggcattgcc tacaagcgaa tcgttttcat accataaaac
    25861 accgaaataa tgacctgtga tatatagatt accctgttca tcgcaatcca taccgttcac
    25921 ttgatcccag tattgtgatc caatggcttt agcccagatg agatctccgt ttggagcata
    25981 tttaaggata cagatatcct caacgaaatt agattcgaat gaatagtttc ctatggtaag
    26041 attcccagta aactgaactg ccataaagga attaccatct ggatcaacgg caactttatt
    26101 gaaattttca aaatcactcg aaacacccgc ccgtggtgac caatttaccc attgaaaatt
    26161 ctgtgatgat aaagttgagg aggcaaaaac catcataaaa ctgatgattg caaaagctaa
    26221 gcgcataagt ttttgtgttg aaatcgagta tcgaaaatag tgtattaaat caactagcag
    26281 tttgaaaatc actcgcttat aaagcaacgg aagatgaatg gattttaccc ttttacttat
    26341 ctaaaatttg agggtactgc atttttagtt tctcgagctt ttctacaatg gtagaggcaa
    26401 tgagataatc cttataccaa ttctgatcgg cagggataat ttgccatgaa gcagctttct
    26461 gacaattttc gaaaatatct tcaaatgcgg ttcgataagc tggccaaagt ttagcttctt
    26521 ctaaatcgga agccttgtat ttccaacgtt tttctggatt attgatgcgt tcttcaaagc
    26581 gctccttttg ttcttcttcc gaaatatgta ggtagaattt aaacacaaga gtacccgaat
    26641 caaccagtaa ttgctcccaa gcatttatgt gtccgaatcg tcgattaact tcttctttat
    26701 caatccattc atgaacacgt ggcacaagca catcttcgta atgcgagcga ttgaataatt
    26761 gaatcattcc tttttcgggt gcatgtggat gaattcgcca aaggaaatcg tgcgactttt
    26821 cttcttcagt tggcgctttg aat
//
LOCUS       DAOOQW010000013        25234 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_2355_length_25234_cov_6.605068, whole genome shotgun sequence.
ACCESSION   DAOOQW010000013 DAOOQW010000000
VERSION     DAOOQW010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 25234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25234
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2355_length_25234_cov_6.605068"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2297
                     /locus_tag="PK281_01765"
     CDS             <1..2297
                     /locus_tag="PK281_01765"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HOY48095.1"
                     /translation="HNLMMKRLFIRPLFYLSFMLIGLTLSSSFRPNYPQGSDPEIDAL
                     IAQMTIEEKVGQMTQLNLDVVCVGEIYKLQEPHRLDPEKLRNALLNWHVGSILNCGGH
                     AYPREQWLQLIGGIQQVAEKETRLKIPVLYGIDAIHGANYVSNSTLFPQQLAQAATFN
                     PEMTRRAAEITAYETRAAGIPWNFSPVLDCARNPLWSRFFETYGEDALMCSRFGEAAI
                     VGYQGAKSNLPIDQTHVAACMKHFLGYGGMRTGKDRTPAYISDIQLREIYLPSFKKAI
                     ESGALTLMINSGEINGIPVHANKLILTNLLRNELGFEGLAVTDWEDIIKLQLNHHVAA
                     NLKEATYLAVEAGIDMCMVPNDFNFSKYLIELVKEGRIPESRLNVSVERILTIKKKLG
                     ILRKSNLPKVKDYPFFNSAQNQILAKNIAAESITLLENRNQTLPLDPMKRILVTGPAA
                     NSMTLLNGAWTRTWQGVDSTFDDRSKNTILEAMRLFAPGNVTYAAGCSVDSFINLQAV
                     LEQSKSCDVIVACMGENPSTEKPGDINDLELSATQQQFVKELIKTGKPVVLVLVENRP
                     RIVRDLVEGCAAVVMAFQPGDYGADALADILVGKINPSGRLPFTYPKFNHSLIWYDHK
                     YTETFDAKYGNNAFQPQWPFGYGLSYSQVSYSDLKLSTNTLHPGEKLTVSLQVTNASD
                     REVKEPVLLFTRDQYASVTPAVRQLKDFTKITLAPKSSQTINFTLDESQLSFIGRDLK
                     PIVEEGDFDVIIGTLQATFKYATK"
     gene            2294..3124
                     /locus_tag="PK281_01770"
     CDS             2294..3124
                     /locus_tag="PK281_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HOY48096.1"
                     /translation="MMKRASILIFLFALSVLSSCKQSDSEEALARTATEVTFSGRKWD
                     IKSGYNGPGPNSFSKAYENVWVDEKGYLHMAITKHEDGNWYSSEVISQDNMGYGTYAF
                     TIQGNPLTFAKNVVLGLFTWDNSTFHDQGNSEVDIEFSKWGVEDDTHPLTTSVQPVFF
                     STYFAERSHQHMIDPELLNGVSTHIFTWTDTLITWKSFKGDDFRSAEPIASWQFDLDH
                     PPRIKEENGEQSLPIVIPAPGPTTNARINFWTQTYIANGPSNGLNHEIVIRKFEYLPL
                     "
     gene            complement(3263..3514)
                     /locus_tag="PK281_01775"
     CDS             complement(3263..3514)
                     /locus_tag="PK281_01775"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HOY48097.1"
                     /translation="MWYYVYILELSNSKHYVGCTVNLKERFNRHSSGRVKSTKPHLPV
                     KLLWCCSFPNRYMAYKFERYMKSGSGRAFASRHLYEMCH"
     gene            complement(3904..3990)
                     /locus_tag="PK281_01780"
     tRNA            complement(3904..3990)
                     /locus_tag="PK281_01780"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3954..3956),aa:Ser,seq:gga)
     gene            complement(4147..5187)
                     /locus_tag="PK281_01785"
     CDS             complement(4147..5187)
                     /locus_tag="PK281_01785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HOY48098.1"
                     /translation="MSRKPSALIIYTGGTIGMVKDSQTGSLKPVNFERLQANIPEIEK
                     LDIELDIYTFENTIDSSNVQPSDWIRLVEVIEKNYDRYDGFVILHGSDTMAYTASALS
                     FMLENLSKPVILTGSQLPIGVLRTDAKENFITALEIAALKEFGMSKVSEVCIYFEYHL
                     LKGNRTTKFSSSHFNAFASTNYPALAEAGVQIRFNDSILRKYNGKPTLFHKKLDTNIA
                     ILKIFPGMTPQYVQAILSIPGLKAIVLETFGSGNAPTEEWFLEAIRQTIESGILVVNV
                     TQCSSGAVVQGKYKTSSDLAHMGVLGGQDITTEAALAKLMFLLANCPDIESVRSQFIL
                     PLREEMTLFDWN"
     gene            complement(5184..5951)
                     /locus_tag="PK281_01790"
     CDS             complement(5184..5951)
                     /locus_tag="PK281_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016200011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOY48099.1"
                     /translation="MSDTHTHLYLPEFDNDRNAMMQRAIEAGVTEIYLPNVDSETIPL
                     MHAMVDAFPSHCFPMMGLHPCSVKPDTMEEELALVKHWLNTREYVAVGEIGLDLYWDK
                     STLDIQIEALKRQCEWALEKNLPVILHCRESTAQSIEVIRPFAERGLRGIFHCFSGNA
                     EEARIITQMGFLLGIGGVLTFKKSGLAEAIAEIPLDFLVLETDAPYLAPVPFRGKRNE
                     AAYIRIILEALSEIRRVGKNELATITTANARRLFKRA"
     gene            6020..7030
                     /locus_tag="PK281_01795"
     CDS             6020..7030
                     /locus_tag="PK281_01795"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOY48100.1"
                     /translation="MAVQRILIIRFSSIGDIVLTTPLVRCLAKQFPNAELHYLTKPTF
                     RSILEPNPYLHKVWLWEDEPKEVLRQLKEQRFDLVVDLHHNLRSKRVKLALRRPSVSF
                     NKLNVEKYLYVNLRINRLPNVHIVDRYLATVAQLGVKNDAQGLDYFIRPEDEYDIKQL
                     PQTHQNGYVAVVTGALKNTKRMPEVELSEVCKRMGLPIVLLGGKAEAEFGAHIASDLG
                     AAAYNTCGEISLGQSASLVKQARVVLTHDTGLMHIAAAFHKPIVSIWGNTVPEFGMYP
                     YLPLASDQQFISQVTDLSCRPCSKIGYADCPKKHFRCMKDQDLTKIAEEVKRMFAFHP
                     NS"
     gene            7109..7357
                     /locus_tag="PK281_01800"
     CDS             7109..7357
                     /locus_tag="PK281_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010182973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HOY48101.1"
                     /translation="MAKNTSILLGDYFDNFINGLVKTGKYSSASEVVRTALRMFEQEE
                     TKKSELIKELIKGEKSGFVSDFNKDSFIENLKEKHLKK"
     gene            7354..7647
                     /locus_tag="PK281_01805"
     CDS             7354..7647
                     /locus_tag="PK281_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016199106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOY48102.1"
                     /translation="MIYKISKEAESDLEKIWLYTIENWSIEQAERYLNLIFDEIEYLC
                     SNPYSGFDFGKIRKGYWRSKVKSHLIFYKVNSKQNELEVIRVLHEIMDIENHV"
     gene            complement(8007..10184)
                     /locus_tag="PK281_01810"
     CDS             complement(8007..10184)
                     /locus_tag="PK281_01810"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY48103.1"
                     /translation="MKNLVPIQNKILFTLVKPLLTLALFILSGNVSNLFAQNWDEVVK
                     AVATDRAAADLFGYSVAISGDYAIVGANQEDHNATGGQFLDNAGAAYILRNNSGTWTV
                     VQKIVAADRDADDEFGTSVAIWGDYAIVGAPRKTATVGFELNSAAGAAYIFKNTAGTW
                     SQLQKIVASDRETGDEFGYSVAISENYVIIGAPYEDEYLNGLSPASNKGSAYIYTKGE
                     TVWMDGQKIVGNSVGNGDNFGWSVGISDDYAVIGTPRESQIASGGGGILTDAGSAYIF
                     RKFSSGTWTQMQSINASDKGAGDEFGTSVAISGDYVIVGAMFEDHNATGGANLSDAGS
                     AYIFEINGGTWTQMDKIVASDREASDNFGASVSISGEYAKVGSYNEDHDQDGGNYIFN
                     PGSAYIFKNTSGNWAQANKIVASERDYEDLFGHSVAISGDYAIVGAFNEDENQAGGNT
                     LGNAGASYVFKNCLARRTDVQTSCGSFNWIDGNTYTANNNTATYNFAGGAASGCDSLV
                     TLNLTINNPAAGVDTRTACNSFTWINGSTYTASNNTATYNIIGGAANGCDSLVTLNLT
                     INSVANLSTTTSGVTITANNTSATYRWLDCNNNYAVIAGQTGASYTPTVNGNYAVQLT
                     QNGCVDTSACVAILTMGIIENSFGDNLVVFPNPTNGYFTIDLGNVFVKSEIQIRDILG
                     NLIETTSVNLSQKLDLFIKEPAGIYIVTVDADNKKAVIRIVKN"
     gene            complement(10709..12805)
                     /locus_tag="PK281_01815"
     CDS             complement(10709..12805)
                     /locus_tag="PK281_01815"
                     /inference="COORDINATES: protein motif:HMM:NF015926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HOY48104.1"
                     /translation="MQTPEESLHAPESGPNEMPEQSTTSSTGDAVNTELSAENAAEVI
                     AETEIPEEVPFTPAPEEIPATEPAPAEESPEPEDTHVEAEIEAPAEVVQENVVSEPIA
                     AEALKDEEEDATEVDLHEDLQPLVAFQELSRIELVAAMQEAVDSEDTDGMRMRVRAIR
                     DGYYKVKDEEVASKRLKFIENGGLAEEFEILRDETDEQFDALVKRFQEKRAEQRRKRE
                     QELITNLRKKEGILAELKAMLENTENISASFDRLHELQEQWRSIGLVPAQYVDELWKN
                     YHHHINNFYDIIKINKELRDLDHKKNLELKTLVCEKAEDLLMEESITKSLDIYKNLQD
                     QWKEIGHVAREHSETIWERFRAAGDKLFDRRREYIQHQEKDYGANLALKNEIVAKAES
                     FLQELPFKSHPQWQEASEKLAALLEDWKKVGFASRKDNEDVWQKFKGIRDQFYAAKEE
                     FYKTLRNAQNHNYKLKVDLCMEAEALRESLDWKKTGERLRQLQESWKTVGPVSKKHND
                     KLWQRFRTACDSFFENRNKHFAGMNDEQDLNLQKKKDLIARVEAFVPGEDTPANFDAL
                     KAFQNEWMEIGHVPIKEKDKLYKAFRTAIDAQFSKLKADSANNRREHFRSQVNSISSD
                     KGGKDKLHHQKVVVQDKIRKLQTDVQTLENNIGFFGNSKSKAAEEMKRDIERKISKAK
                     EEIGQLHDQLRILQEV"
     assembly_gap    12878..12977
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            13061..15385
                     /locus_tag="PK281_01820"
     CDS             13061..15385
                     /locus_tag="PK281_01820"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY48105.1"
                     /translation="MILNYRLFFLSISLFFLFQSVKAQNTGNVAIGEWREHLPFSRGI
                     SVCQNDKFVYCATNNGVVIYKKDDGTTERLSRSTGLTDINLSCVNYHEQSSTLVIGYK
                     NGNLDLVRNNEVVNVGDIKRSTVVQGGKTINAIRFINDRAYLCCNFGIVVYDMTNREV
                     RTTLYPSLLNPDIHDVVFDGVNTIYAATSKGLFSADINNPALPYYVAWTQDPVIGTVP
                     LNNLTWFNGAIYTSRYVDNAQDQDTLYQLKDGQLEVFRTGQTIHSINSRPERMVYTTN
                     YGATMVDSGSLYIENVLSYSDILLPPSPLDAVIDLVDVKRVWIADKNQCLVKSQNVFI
                     NTPFSVEGPPTSNVFRLASSAGKVWVAPGAWDVAYSPLYSIDGVFEFDGLNWKDYKLN
                     YGSDFIRDIVSIVIDPADASHVYAASWGGGIAEIKNGSIVQQYAATNSGLTGIANYPN
                     DIRVSDLTIDKDGNLWSTCSSSSKPLAVKTPGGEWKNFAFNNSVNNQFLGNMLVDSSG
                     LKWVIVQERGLLVANFDGTTLEGYKFLNDQVGNGALASSSVLCLAQDLDGQIWVGTTK
                     GISVFYTPEAIFQEGSTGWDSQTIIISQDGFNQYLLKEEEVTAIAVDGANRKWIGTKK
                     AGIFVVSPDGTQQIAEFNSENSPLLSNNVTSLSINGESGEIFIGTDLGICSYRTDASR
                     GGDKFGHVYTFPNPVEHGYEGPIAITGLVTDADVKITDVSGNLVFSTVANGGTAIWNG
                     KLYSGQRAATGVYLVFCTNEDGSQTKVTKFLFIN"
     gene            15388..16107
                     /gene="recO"
                     /locus_tag="PK281_01825"
     CDS             15388..16107
                     /gene="recO"
                     /locus_tag="PK281_01825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HOY48106.1"
                     /translation="MLLASQAIVLHTFRHGDSSVIARMYTRERGLVSFLIHGAKSKKG
                     GNKTALLQPLSLLEVQMQLKENRGLQQLREMRIDFPYSGIPFSTGKTAQVLFLAEVLI
                     HTIKEEEQNDQLFDFLRDALILLDQTPSELPDFHLKFLLEFSRFLGFYPVENYCEATP
                     YFQFTDGVFHHSPNSTCFDLPTSAALNQLMITGFGSLQTGLFNRPMRRQVSRKLLEFF
                     RWHLPSISGLQTPEVLEEVFG"
     gene            complement(16071..17213)
                     /locus_tag="PK281_01830"
     CDS             complement(16071..17213)
                     /locus_tag="PK281_01830"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOY48107.1"
                     /translation="MTSTAKQSNYFENLDSTRFLGFFHVFLAHCFFTTNPAIQESFGY
                     HLATVNIRSGFLGLDYFFVLSSFLLTWLALKEWQQTGSFQPGLFMVRRGLRLWPLYLV
                     LVGGTYGFVYFWGDKLGISTLPPVEVFLLFVSNWWIAAHGQNFLFLLVFFWSIAVEEQ
                     FYVFWAFVLRFLHRNILWVSGGMIVVSLVFRAMNLSDEPRLYFNTISYLGNFGLGALC
                     AQLAFDNGRFIRVIERLSKRHIIVVYLLLAVLTAGYFRWFQSPVMVVLEKLIFGAFFC
                     FIILEQSFALHSPIKLGKFKRMSYLGQLSLGLYCFHGIVLTFAIPLLKSSGYAESTWQ
                     VYVMNPLLILSATLILAWLSFVGFEQPIHRYRKYFYPKTSSSTSGV"
     gene            complement(17210..18268)
                     /locus_tag="PK281_01835"
     CDS             complement(17210..18268)
                     /locus_tag="PK281_01835"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOY48108.1"
                     /translation="MKCDFLIIGQGIAGTTLALELISRGSEVLVIDVFDEHNSSRVAA
                     GLFNAVVFKRITLGWRAKESLKEAHLFYAQQEEFTGVRLFYPIGLHRIHGSEAERKSW
                     IDVSKDPEFVGMLEESKPADEKPWLKQTFGGAHVPGAGFIDTRTWLSAVRRKFLEKNI
                     LREEIFDYDALEVSPDGVIYKDIEAPKIIFCEGVGATKNPWFNDLPFNPAKGEVLVIY
                     APELKHEIFNGSIYGVPLGDGMFRVGSTYEWGTTDPNPTQDKRLDLEEKLRNILQVPF
                     TVVLHEAGVRPTVLDRRPLIGLHPRHQALGIFNGLGTKGVLMAPLQAKEFVSFLLDGA
                     SLSPEASIQRFVKRLTRQ"
     gene            18340..19104
                     /locus_tag="PK281_01840"
     CDS             18340..19104
                     /locus_tag="PK281_01840"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOY48109.1"
                     /translation="MLRLLSYILLIPYWIWLILVFLCFLVSSSLIGLILIPTIGKRAH
                     QPMMMVYKFWSATFFFCAGIRIKTINREKLFGNLPCIVVSNHGSNLDMFIGTYSLPLS
                     VKPLAKVQLKKMPLLGFLFATVCVLVDRRSKESRDKSSRAMMSEILSGNSIFIFPEGT
                     RNKSQEPLNEFFDGAFRFAIESQKPLVAMCSINARNITPSDNYAVRPGTITIKFLGPY
                     ETTGLTKEDLPALKTKIREEMYQVLKTEDPMFKHLL"
     gene            complement(19099..21723)
                     /locus_tag="PK281_01845"
     CDS             complement(19099..21723)
                     /locus_tag="PK281_01845"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HOY48110.1"
                     /translation="MFKQLVTIVFVLISYCGYSQLQDDFADGNFSSNPVWSGNASEFL
                     VDGTFQLQSNGDTTSLSNREIYLSTPSTSINNTQWEFLVNPKVSTSSNNRMDVFLTSS
                     DANLLGANTGYFVRIGGTPDEVALFRKDGAGIESYVITGVAGVINSSSTNPTKVKVTR
                     DVAGNWSLYADYNGTGALYELIGTGQDVTYTTSLSFGLVVRYSSSNRQKYFADNFYVG
                     PIIVDNIPPVVQSIKVLSDHALELSFSESVSQTTAEETANYLVNSSIGEPSSAFRSLT
                     SFSKVTLTFIPAFQEGITYQLTVSAIEDLSGNDMNPQQIPFIYYRPKPYDVVINEIMA
                     DPDPSAGLPSVEFIEVANRTAFPINLENWMIEAGTNRKVIPACILQPDSFIVLTGTDG
                     LEYYSDSIAVVGITSFPALTNTGARLTLYNPDTVVISSVTYADSWYQNTVKSEGGYSL
                     EQISPMQPCAGAENWIASSNNWGGSPGKINSVYSNVSDQQAPKIDRVVVIAVDTIRVF
                     FTESIRLSSQQDLNSYTIDQGIGQPNYIETYSPDFKSVKLALTTSLQTDVLYTISISS
                     DLFDCAGNAFDVQSTGRFAIPQIPQANDIVINELLSNEKDGASDFVEIYNRSNKIIDL
                     ASLQLSQYDTLVQVPIDLEFIAPDGYLLFPGEYLVLTDDISGVKNFYSTTNPSGFIQM
                     SSMPTMNNDDGTLALSRVSDNAIIDRMNYNTSMHFALLNNLDGVSLERVNYDRPALDV
                     TNWNSASSAAGFATPAYRNSQYSEVVTSQPGSVTISPEVFSPDGDGYEDVVNFAYAFD
                     TPGFTGTLSIYDSNGRLVKYIGRGILLGTSGQYSWNGINEDNEKARLGIYIFVLDAFT
                     LDGQVSKIKKSFVLGGRL"
     gene            21802..22797
                     /locus_tag="PK281_01850"
     CDS             21802..22797
                     /locus_tag="PK281_01850"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010135441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOY48111.1"
                     /translation="MKVAVVGATGLVGNKMLQVLQERNFPVTNLIPVASERSAGRRIA
                     FQQDEWTVLTPENALLLQPDLALFSAGGSTSLEWAPRFAEIGCTVIDNSSAWRMDPSK
                     KLVVPEVNAHVLEASDKIIANPNCSTIQMVVALQPLHQKYKIKRIVVSTYQSVTGTGL
                     KAVQQLINERKGTLGEPAYPHPIDLNILPHIDSFLDNGYTKEEMKMLNETRKIMGDDS
                     IRVTATTVRVPVIGGHSESVNVEFEQDFDLQQVRELLAAAPGVIVEDDVLHNRYPMPI
                     NANNRDEVFVGRLRRDESQPNTLNMWIVSDNLRKGAATNAVQIAEYLMNKGWLTA"
     gene            complement(22798..23424)
                     /locus_tag="PK281_01855"
     CDS             complement(22798..23424)
                     /locus_tag="PK281_01855"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013069692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HOY48112.1"
                     /translation="MKKSLIILLSVLAIDQTVKFLIKTNLMLGESIQVAGHWCYLTFV
                     ENNGMAFGMEFGGEFGKLFLSLFRIVVVGTMCYYLLKASKEKKLHPRLQVSFSLIIAG
                     AIGNIIDSAFYGLIFNDSFGQVASLFPTEGAYGTFLHGRVVDMFYFPLFEGHFPSWSP
                     VYAGEEFIFFSPVFNVADFSISIGAVIFLFYQKHFFTEKKKEDPVVEA"
     gene            complement(23473..23853)
                     /locus_tag="PK281_01860"
     CDS             complement(23473..23853)
                     /locus_tag="PK281_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019669190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HOY48113.1"
                     /translation="MNQQEKNRYNPEELQEFKEIIEKKLDEARKDYDLLKSAFSNKDD
                     HGTDDTSPTFKLLEEGSDVLSKEETGQLAARQKKFIENLEAALIRIQNNTYGICRVTG
                     KLIPKERLRIVPHATLSIEAKNQQ"
     gene            complement(23850..>25234)
                     /locus_tag="PK281_01865"
     CDS             complement(23850..>25234)
                     /locus_tag="PK281_01865"
                     /inference="COORDINATES: protein motif:HMM:NF039585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5915 domain-containing protein"
                     /protein_id="HOY48114.1"
                     /translation="KMSKRLGNGVDPFETLGKYGPDATRWYMIGNAQPWDNLKFNVEG
                     ITEVQRKFFGTLYNTYAFFALYANIDGFKMDASKQIPLADRPEIDRWVISRLNSLKKE
                     VAERMDDYDPTPAVRAIEDFVTEQLSNWYVRLGRRRFWRSESDSDKQAAYETLYECLE
                     VVSQLISPVAPFFGEWLYRNLHVHEINQSLVSVHLSTWPAVNSDFIDSDLEERMGLAQ
                     RYSSMILGLRKKVNIRVRQPLQKILVPVIDPHIKTQLNAVKDLILSETNVKELEYVGD
                     DAGVIEKKIKPDFKALGPKYGKLMKSIAAVVNTLNTPQINELQREGRLAVDVEGQEIV
                     LSPEDVEIFTEDIPGWLVASEGPYTVALDVTLSESLVEEGLARELVNKIQNLRKVSGL
                     EVTDTIEIIVQNHPALTAAIENNKTYICAETLGASLTIVNNGAASGGTLVELTDEIST
                     LITISKLNGL"
BASE COUNT         7286 a         5469 c         5140 g         7239 t
ORIGIN      
        1 gccataattt gatgatgaaa cgtttgttta tccgaccgct tttctatttg agtttcatgt
       61 tgattggatt gacgctgagt tcgtcctttc ggccaaatta tccgcaagga agcgatcccg
      121 aaattgatgc cctgattgct caaatgacca tcgaagaaaa agtgggtcag atgacacagc
      181 ttaacttgga tgtggtgtgc gtgggagaga tttacaaatt gcaagaacca catcgtctcg
      241 atcccgaaaa actgcgcaac gccttgctaa actggcatgt tggttctata ctcaactgtg
      301 gcggacatgc ttatccacgc gaacaatggt tgcaactcat aggaggaatt cagcaggttg
      361 ccgaaaaaga aacacgcttg aaaatccccg ttttatatgg gattgacgca attcacggag
      421 caaactatgt gagtaattct accttgtttc cacagcaatt ggcgcaggct gctacgttta
      481 atcccgaaat gactcgtcgc gctgctgaaa tcacagctta cgaaacacga gctgcaggaa
      541 ttccctggaa cttttctcct gtgttagatt gtgctcgaaa tccactttgg tcgcgctttt
      601 tcgagacata cggcgaagat gccttgatgt gttcacgttt tggtgaagct gctattgttg
      661 gttatcaagg cgcgaaaagc aatctgccca ttgaccaaac acacgtggct gcttgcatga
      721 aacattttct gggctatggt ggaatgcgaa ccggtaaaga ccgcacacca gcctatattt
      781 ccgacataca gctgcgcgaa atttatctgc catctttcaa gaaagccatt gaaagtggag
      841 cgttaacttt aatgattaat agtggtgaga ttaacggaat tccggttcat gcaaacaaac
      901 tcattctaac caatttgctt cgcaacgaat tgggtttcga aggtcttgca gtaaccgatt
      961 gggaagatat tatcaagcta caactcaatc accacgtggc tgccaactta aaagaagcga
     1021 cttatttggc cgttgaagca ggcatcgaca tgtgcatggt gccgaacgac tttaatttct
     1081 ccaagtacct aatcgaattg gtcaaagaag gacgaattcc ggaatctcgc ttgaatgtat
     1141 cggtagagcg cattttaacg atcaagaaga agttggggat tttacgcaaa tcgaatcttc
     1201 ccaaagtgaa agattacccg ttttttaatt cagctcaaaa tcaaatctta gcaaaaaaca
     1261 ttgcagcaga atcgattaca ctgctcgaaa atcgcaatca aaccttaccg ctcgatccca
     1321 tgaaacgcat tcttgttacc ggtccggctg cgaattccat gaccttactc aacggagctt
     1381 ggacgcgcac ttggcaaggt gtcgattcca cattcgacga tcgttctaaa aacaccattc
     1441 tcgaagccat gcgccttttt gctcccggaa atgtgacata tgcagcgggc tgttctgtag
     1501 atagttttat caatttacaa gcggtattgg agcaaagtaa atcttgcgat gtaattgtgg
     1561 cttgcatggg tgaaaatcca tctaccgaaa aaccgggcga catcaacgat ttggagcttt
     1621 ccgctacgca acagcaattc gtaaaagagc ttattaaaac tggaaaaccc gttgttttgg
     1681 tattggtaga gaatcgtcca cgcattgttc gggatttggt agaaggatgt gccgctgttg
     1741 ttatggcatt tcagcctgga gactatggcg cagacgcatt agctgatatt ttggtaggca
     1801 aaataaatcc aagcggtcgt ttaccgttca cgtatccaaa attcaatcac agtcttattt
     1861 ggtacgacca taaatacacc gaaaccttcg atgctaaata cggaaacaat gcttttcaac
     1921 cgcagtggcc gtttggatat ggcttatcct attcgcaagt aagctattcc gatttaaagc
     1981 tttcaaccaa cacgctccat ccaggtgaaa aacttacggt tagtctacaa gtaacgaatg
     2041 ccagcgatcg agaagttaaa gaacccgttt tactttttac ccgagatcag tatgcatcgg
     2101 taactccggc tgttcgacaa ttaaaagatt tcaccaagat tacacttgct cctaagagct
     2161 ctcaaaccat caattttaca ctagacgaat cgcaattgag ttttatcggc cgagacctga
     2221 aaccgattgt tgaggaaggc gattttgatg tgattattgg tacacttcaa gccacattta
     2281 agtacgctac aaaatgatga aacgtgcatc catactgatt ttcctgtttg ctctgagtgt
     2341 tttatccagt tgcaaacaat cggattccga agaagcatta gcgcgaacag ctaccgaagt
     2401 cactttttcg gggcgtaaat gggatattaa atcgggttat aacggtcccg gtccgaacag
     2461 tttttccaag gcttacgaaa atgtttgggt ggatgaaaaa ggctatctgc acatggctat
     2521 taccaagcac gaggatggaa attggtattc gtccgaagtt atttcgcaag acaatatggg
     2581 atatggaact tatgccttca ccatccaagg aaacccgctt acgtttgcta aaaatgtggt
     2641 tttaggatta tttacttggg acaattcaac ctttcacgac caagggaata gcgaagtcga
     2701 tattgagttt tcgaaatggg gcgtagaaga cgacacgcat cctttaacga cttcggttca
     2761 gccggtattt ttcagtactt acttcgccga acgcagccac caacacatga ttgaccctga
     2821 attgttaaat ggtgtaagca cgcacatctt cacctggacc gacacgctca tcacctggaa
     2881 aagctttaaa ggcgatgatt tccgcagtgc cgaacccatt gctagctggc aattcgattt
     2941 ggatcatccg ccgcgtatca aagaagaaaa cggagaacaa tcgctcccaa tcgtcatccc
     3001 ggcacccggt ccaaccacca atgcccgcat caacttctgg acacaaacct acatcgccaa
     3061 cggtccgagc aacggcctta accacgagat tgtaattagg aagtttgagt atttgccgct
     3121 gtgaggtagt tctcatttgg ggcctccctt tccgtttcac ttcaaggtcg ggtgctcgcc
     3181 catacacggt atttggctcg ccccctaagc cgaggaacag tgctttttct ggatattgga
     3241 atcaccttac ttaagtcttg cttcaatgac acatttcgta tagatgtctg gaagcaaacg
     3301 cccttcccga accagacttc atataacgct caaatttata agccatatac ctattcggaa
     3361 aagaacaaca ccaaagtagt ttgacaggta aatgaggttt tgttgatttt accctaccac
     3421 tcgaatgtcg attaaagcgc tcttttaaat taactgtaca gccaacataa tgcttactat
     3481 tacttagctc taggatataa acataatacc acataatgaa taatatatcc actaaaataa
     3541 aaaaaaaccc agtaaagatg aaatccttac tgggaatgtg tccaccttct aagactaacg
     3601 ccctccttcg cgggttatac ccgcttcggc gggcaatgcg ggcaatgccg acaaagcaat
     3661 atttcgtaga cataaaaaac ccagtaagga taaaatcctt actgggaatg tgtccacctt
     3721 ctaggactaa cgcccacctt cgcgggttat acccgcttcg gcgggcaatg cgggcaatgc
     3781 cgacaaagca atatttcgta gacataaaaa aacccagtaa ggataaaatc cttactggga
     3841 tgtgcccact ttcgaagact aacgcggatg gtatccgcca tagtccgctt cggcggacga
     3901 aggcggagac tgagggattc gaaccctcga tacagttacc cgtatacaaa ctttccaggc
     3961 ttgctccttc aaccactcgg acaagtctcc atttttacta cttcaatcta tattcttgtg
     4021 agatttcccg aaaagtcagg acaagctctc gaacaagtat cgaattaggg acggcaaaac
     4081 taggaaaaaa aacgttccgg gaacctattt cataagcaga agcgcctcat tgtaattaag
     4141 aaagcttcaa ttccagtcga aaagcgtcat ttcttcgcgc aatggtagga tgaattgtga
     4201 gcgaacgctt tctatatcgg gacaattagc taataggaac atgagcttcg ctaatgcagc
     4261 ttcggtggtg atgtcttgtc cgccgagaac gcccatgtga gcaagatcgg agcttgtttt
     4321 gtacttgccc tgaacgaccg cgccactgct acattgagtc acgtttacga ccaaaatccc
     4381 actttcgata gtttgacgaa ttgcttccaa gaaccattct tcggtaggag catttccgga
     4441 gccgaatgtt tctaaaacga tagcttttaa acccggaatg gacaagattg cctgcacata
     4501 ctggggcgtc attccaggga aaatcttgag aatggcgatg ttggtatcga gtttcttgtg
     4561 aaagagagtg ggcttgccgt tgtatttccg caagatggaa tcgttgaaac gaatttgcac
     4621 tccagcttca gctaatgcag gataattggt gctagcaaaa gcgttgaagt gactcgaact
     4681 gaacttagta gttcggtttc ctttgagcaa atgatactca aaatagatac acacctcgct
     4741 tactttactc atcccaaatt ctttgagagc agctatttcg agcgcggtga tgaagttctc
     4801 tttagcatct gtacgaagta ccccaatggg taattgcgag ccagtaagaa tcacgggttt
     4861 gctcaaattc tcgagcataa aactcagtgc cgacgcggta tatgccatgg tatcggaacc
     4921 atgaagaatc acgaaaccat cgtagcgatc atagtttttt tcaatcactt cgaccaatcg
     4981 aatccaatcg ctaggttgca cattagaaga gtcgatagtg ttttcgaacg tgtagatatc
     5041 tagctcaata tcaagctttt cgatttcggg aatgttagcc tgaagtcgtt cgaagttcac
     5101 gggtttaagc gagccggttt gactatcttt caccataccg atggtaccac cggtgtatat
     5161 aattaatgca gatggttttc tgctcatgcg cgtttaaata gtcggcgggc gtttgccgtg
     5221 gtgatagttg ccagttcgtt ttttcctacg cgacgaattt cagaaagggc ttccagaata
     5281 atccgaatat aagccgcttc gtttcgtttg ccacgaaagg gaacaggagc aagatacggc
     5341 gcatcggttt cgagcacaag aaaatcgagc gggatttctg caatcgcttc tgccagacca
     5401 cttttcttaa aggtaagtac accaccaatg cctaataaaa agcccatttg ggtaatgatg
     5461 cgcgcttctt ctgcatttcc cgaaaagcaa tggaaaattc ctctcaagcc acgttcggca
     5521 aacggacgaa tcacttcaat actttgagca gtcgattcgc gacaatgcaa aatcaccggt
     5581 aaattcttct ccaaagccca ttcacactgc cgttttaagg cttcaatttg aatgtcgagg
     5641 gttgatttat cccagtacaa atcgagccca atttcaccta cagccacata ttcacgtgtg
     5701 ttgagccaat gtttaactaa ggctaattct tcttccattg tatcgggctt taccgaacaa
     5761 ggatgcaagc ccatcatagg aaagcaatgt gatggaaaag catccaccat ggcgtgcata
     5821 agcggtatgg tttcgctatc cacattgggt aaataaatct cggtaacgcc cgcctcaatt
     5881 gcgcgttgca tcatggcatt gcggtcgttg tcgaattcgg gaagatacaa gtgtgtatgt
     5941 gtatcgctca aagtatgcat gctgcaaagg tgcagaattc aatcgaagca gaacaaggct
     6001 gcgaaaaacc attagttttg tggctgtgca gcgcatctta attattcgtt ttagctccat
     6061 tggcgacatt gtgctcacca cgccactcgt gcgttgtttg gccaagcagt ttcctaatgc
     6121 agagttgcat tacctcacca agcctacatt tagaagcatt ctcgagccca atccgtattt
     6181 gcataaagtg tggctttggg aagatgaacc caaagaagtg ctgcgtcaat tgaaagaaca
     6241 gcgtttcgat ttagtagtcg atttgcatca taacctgcgc agcaaacggg ttaagttagc
     6301 gttacgtcga ccatcagttt cgtttaataa actcaacgta gagaaatatc tgtacgtcaa
     6361 ccttcgcatc aaccgcttgc ccaatgtgca tattgtagat cgctatttgg ctacagtggc
     6421 tcaattagga gtgaagaacg atgcacaagg actcgattat tttattcgtc ccgaagatga
     6481 atatgatata aaacaattgc ctcaaacgca tcagaacggt tatgtggcag tcgttaccgg
     6541 agcattaaaa aacaccaagc gtatgcccga agtggaactc agcgaggtgt gtaagcgcat
     6601 gggtttgcct attgtgctct taggtggaaa agctgaagcc gaatttggtg ctcacattgc
     6661 cagcgatttg ggcgcagcag cctacaatac ctgtggtgaa atctctttgg gacaatcagc
     6721 ctccttggta aaacaagcac gcgtggttct tacgcacgat accggtttga tgcacattgc
     6781 cgccgcattt cacaaaccca ttgtatctat ctggggcaat accgttcccg agtttggtat
     6841 gtatccttat ttgcctctag cgagcgacca gcaattcatc tcccaagtaa ccgatttatc
     6901 ctgccgacct tgctctaaaa ttggctatgc tgactgtcct aaaaaacatt tccgctgcat
     6961 gaaagaccaa gatttgacca agattgcgga agaggtgaag aggatgtttg cttttcaccc
     7021 aaattcataa tgctcatcca gcatagcaaa attaggtgaa acgcaacttg caataccatg
     7081 aattggtata tttgaaaaaa agaatgtcat ggcaaagaac acatcaattt tactaggaga
     7141 ttactttgac aatttcatta acggattggt aaagactggg aaatattctt ccgccagtga
     7201 agtagttcgc acggcattaa gaatgtttga acaagaggaa acaaagaaat ccgagcttat
     7261 taaagaactt atcaagggtg aaaaatcagg ctttgtctca gattttaata aggattcatt
     7321 tatagaaaat ctgaaagaaa agcatttaaa gaaatgatct ataagattag taaagaagca
     7381 gaaagtgatt tagagaagat ttggctttat acaatcgaaa attggtcaat tgagcaagca
     7441 gaaagatatt tgaatttaat ttttgatgaa attgagtatc tctgctctaa tccatattca
     7501 ggatttgatt ttggaaaaat tcgcaaagga tattggcgct caaaagtcaa atctcattta
     7561 atattttata aagtaaatag taagcaaaac gaattagaag tcattcgtgt tttacatgaa
     7621 ataatggata ttgagaatca cgtataagct cgatatacga tcgcttcgct atacgatgta
     7681 cgatttacga gaaaatctgc taggttgaag aatttcgttt agggattgtc atttgaaaac
     7741 gaatgtttgt cctatcgaat ttcgtaatac ttcgtactca atacttaata cagacttacg
     7801 atcgcttcag atttaaaaga gaaaagttcc gcacacaccc gaagagccaa atgaggtgaa
     7861 ctgaatgctt tagattagat gaatacaaaa agaaaaacga atgcatcaag ctgccgaaat
     7921 gcttgtcttt gctgatatgt gttgctcgtt aaagttattg aataggctgg tttctaaatt
     7981 tcacttttca agcgtcgtaa atcagtttaa tttttcacta ttcgaatcac cgcctttttg
     8041 ttatccgcat caacagtgac aatgtaaatt ccagctggct cttttatgaa aagatctaat
     8101 ttttgcgaaa gattcactga cgtagtttca attagattac ccagtatatc tctaatctgg
     8161 atttctgatt tcacgaaaac atttcctaga tctatagtga aataaccatt tgtaggatta
     8221 gggaaaacca caaggttatc tccaaaactg ttttcaataa tccccatcgt taaaatagcc
     8281 acacaagcag atgtgtctac acacccattt tgagttaatt gaacagcata atttccatta
     8341 acagtaggtg tgtaggaggc gccagtttgc ccagcaatca cagcataatt attattacag
     8401 tcaagccaac ggtaagttgc agatgtgtta tttgctgtaa ttgttacccc tgatgttgtt
     8461 gtgctcaggt tcgcaacact gttaatagtc aaattaagtg taaccagaga atcacagcca
     8521 ttcgctgcac caccaatgat attgtatgta gctgtgttgt tgcttgctgt ataagtgctt
     8581 ccattaatcc aggtgaagga attgcatgct gttctggtat caactccagc ggcaggattg
     8641 ttaatcgtta gattcaaagt aaccaatgaa tcgcatccac tggctgctcc tcctgcaaaa
     8701 ttgtatgttg cagtgttgtt gtttgcggta tacgtgtttc cgtcaatcca attgaatgaa
     8761 ccgcaagaag tttgaacatc cgttcttctg gcaagacaat ttttaaatac ataggaagcg
     8821 ccggcattgc caagggtatt tcctcctgct tgattttcat cttcattaaa ggcaccaact
     8881 atcgcataat ctccagaaat ggctacagaa tgaccaaaca ggtcttcata atctctttcc
     8941 gaagccacaa tcttgtttgc ctgcgcccaa ttccctgagg tatttttaaa gatataagct
     9001 gaacctggat taaaaatgta attcccacca tcttgatcat gatcttcgtt ataggaacca
     9061 acttttgcat actctccaga aatggacact gaagcgccaa aattatcact tgcttctctg
     9121 tctgatgcca caatcttatc catttgtgtc caagtcccac catttatttc aaaaatatat
     9181 gcagaaccag catcactcag gttcgctcct ccagttgcat tgtggtcttc aaacattgca
     9241 cccacaatta cgtaatctcc cgaaatggcg actgaagtgc caaattcatc gccagcccct
     9301 ttgtctgaag catttatcga ttgcatttga gtccaggttc ctgaggagaa ttttctaaaa
     9361 atataagcag atccagcatc agttagtata cctcctccac cagaagcaat ttggctttct
     9421 ctcggcgtcc cgatgaccgc atagtcatca gaaataccaa cagaccaacc aaaattatca
     9481 ccattaccta cagaatttcc cacaattttt tgcccatcca tccaaacggt ttcccctttt
     9541 gtatagatat acgctgaacc cttatttgat gcgggagaga gtccattcaa atactcatct
     9601 tcataaggag ctcctatgat aacatagttt tccgaaatgg ccacagaata gccgaattca
     9661 tcaccagtct ctcggtctga ggccacgatt ttttgcaact gactccaagt gcctgctgta
     9721 ttcttaaaga tatatgcagc tccggcagcg gaatttaatt caaatccgac tgtagcggtt
     9781 tttctaggag caccaacaat ggcataatct ccccagatag ctaccgaagt tccaaactcg
     9841 tcatcagcat cgcgatcggc agctacaatc ttttgaacta ctgtccatgt accggaatta
     9901 tttctaagaa tatatgcagc accggcatta tcgagaaact gaccacctgt cgcattatga
     9961 tcttcttgat tagcgcccac tatagcataa tcgcccgaaa tcgccacaga atatccaaac
    10021 aaatcagcag cggctctatc cgtagcaact gcttttacaa cctcatccca attttgggca
    10081 aataggttgc taacattgcc cgatagaata aatagggcta atgtcaagag tggttttacc
    10141 aaagtaaaaa ggattttatt ttggattggt actaggtttt tcatgaaatt gtgatggtag
    10201 gattcagcta ggcagattct cttatacgaa gctaatctat aatgttaaat tggaattaaa
    10261 tagggagaag cttaaaacat tctttttgat taggagtccg ttctgatgac tttgaaaagt
    10321 caacgctctg ccaattgagg agtgtttttt agtttaaaaa ggattttcta atcatggaaa
    10381 aactcaagac gagtaacaca taacttctat gctgaagaat ttcgcttaga gacttagatt
    10441 tgaaaacgaa tgcttgtccg atcgaattta tcgtaaatcg ttctacgtaa atcgtacatc
    10501 gcttcagaca tacgatcgct tcactatacg atgtgcgatt tacgagaaaa actgctaaga
    10561 ggaagaatac cgattaggga atttagattt gaaaacgaat ttaagtcctc gcaaaccttt
    10621 cgtaaatcgt aatacgtata tcgtacatca ttccacaaaa aaagcccctc caattgcttg
    10681 aaaaggcttc tcattagtat catgcagctt atacttcttg aaggatgcgc aactgatcgt
    10741 gtagttgtcc aatctcttct ttcgctttgc tgattttgcg ttcgatatcg cgtttcattt
    10801 cttcggcagc tttcgactta gaattaccaa agaagccaat gttgttttcg agcgtttgca
    10861 cgtcggtttg cagcttgcgg attttatcct gaacaacaac tttctggtga tgcaatttgt
    10921 cttttccacc tttgtcagag ctaatgctgt taacctgtga acggaaatgt tcccgacggt
    10981 tgttggcact atcagctttt aatttagaga attgtgcgtc gattgctgtg cggaatgctt
    11041 tgtaaagctt gtctttttcc ttaattggaa cgtgaccaat ctccatccat tcgttttgga
    11101 aggctttcaa cgcatcgaaa tttgctggag tatcttcacc tggaacaaat gcttctacac
    11161 gagcgattaa gtctttcttc ttttgaagat tgagatcttg ctcatcgttc attcctgcaa
    11221 aatgcttgtt tctgttttcg aagaatgaat cacaagccgt gcggaaacgt tgccacaatt
    11281 tatcgttgtg tttcttagaa actggaccaa ccgttttcca agattcttgt agctgacgta
    11341 agcgttctcc tgtttttttc cagtctaaac tttcacgcaa agcttctgct tccatacaca
    11401 aatcaacttt taacttgtag ttgtgatttt gtgcgttacg tagggtttta tagaattctt
    11461 ctttcgccgc gtaaaactga tcgcgaatac ctttgaattt ttgccaaacg tcttcattgt
    11521 ccttgcgaga agcaaatcca actttcttcc aatcttctag taaggcagct aacttttcag
    11581 aagcttcttg ccattgtgga tgactcttga atggtaattc ttgaaggaaa gattcagctt
    11641 ttgctacaat ctcatttttc agtgctaggt tagcaccgta gtctttttct tgatgttgga
    11701 tatattcccg acgacgatcg aacaatttat ctcctgcagc acgaaagcgt tcccaaatgg
    11761 tttcgctgtg ttcgcgtgca acatgaccaa tttctttcca ctggtcttgc aagtttttat
    11821 aaatgtcgag tgatttggta atcgactctt ccatcaataa atcctcggct ttttcgcaaa
    11881 ccaatgtttt taactcgaga tttttcttgt gatctaaatc gcgaagttcc ttgttgattt
    11941 taatgatgtc gtagaaattg ttgatgtgat gatggtagtt tttccataac tcatctacat
    12001 actgggctgg aactaagcca attgaacgcc actgttcttg taattcatga aggcgatcga
    12061 agctagccga aatattctcg gtgttttcga gcattgcttt caactcagct agaattcctt
    12121 cttttttacg caagttggtg atgagttctt gttcacgttt gcgtcgttgt tcagcacgtt
    12181 tctcttgaaa acgttttact aaagcatcaa actgttcatc ggtttcatcg cgaagaatct
    12241 caaattcttc agccaatcca ccattctcta tgaatttgag gcgtttcgaa gcaacttctt
    12301 catctttaac tttgtagtaa ccatcacgaa tcgcccgaac gcgcatacgc atgccatcgg
    12361 tatcctccga atcaacagct tcttgcatgg ctgcaactaa ctcaatccga gataactcct
    12421 gaaatgcaac taatggttgt aaatcttcat gcaaatctac ctctgtagca tcttcttctt
    12481 catctttcaa cgcttcagca gcaataggtt ctgaaacaac attctcctga acaacttccg
    12541 ctggagcttc tatttcagct tccacatgag tatcctctgg ttctggactt tcttccgccg
    12601 gagccggctc tgtcgcagga atttcctcgg gtgcaggtgt aaatggaact tcttccggaa
    12661 tctccgtttc tgctatcact tcagcagcat tttcggctga aagctcagtg tttacggcat
    12721 ctccagtaga agaagttgta gattgttccg gcatttcatt cgggccggat tccggtgcgt
    12781 gtagcgattc ttcaggtgtt tgcatgggtc aaaaatagcc ttttccgctc agcgttgtgt
    12841 tagcggacat ttaaaagtaa ttgaatttaa cgcatgannn nnnnnnnnnn nnnnnnnnnn
    12901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12961 nnnnnnnnnn nnnnnnnctt cttcctttat ccttaatcgt tctttcttca aataaaatat
    13021 ctttacaccc agcgcgttta tcgaagctga cttacatttt atgattctga attaccggct
    13081 ctttttcctt agcattagtt tattcttcct gtttcaaagc gtgaaagctc aaaacacagg
    13141 aaatgttgcc atcggcgaat ggcgcgaaca tcttcctttt agccgaggaa tttctgtctg
    13201 tcaaaacgat aagttcgttt actgcgcgac taataacgga gttgttattt acaagaaaga
    13261 tgatggtaca accgaacgac tttctcgctc aacgggtttg accgacatta atctttcctg
    13321 tgtcaattac catgagcaat cgagcacctt ggttattgga tacaaaaacg gaaatctcga
    13381 tttagttcgg aataacgaag tggttaacgt gggcgatatt aaacgctcaa ccgttgttca
    13441 aggcgggaaa accatcaatg ccattcgatt catcaacgac cgagcatact tatgctgcaa
    13501 ttttggaatt gtggtctacg atatgaccaa tcgcgaagtc cgcactacgc tatatccttc
    13561 tttactcaat cccgacattc acgatgtagt ttttgatggc gtaaatacaa tctatgcagc
    13621 tacctcaaaa gggctatttt ctgccgacat caataatcct gcattgccct attatgttgc
    13681 ttggacacaa gatccagtta ttggtactgt gccactcaat aatctaactt ggttcaatgg
    13741 agcaatctat acttctcggt atgtcgacaa tgcacaagat caagacacct tgtaccaatt
    13801 aaaggacggt cagctggaag tgttccgaac aggtcaaaca attcattcca taaattctcg
    13861 acctgaacgt atggtttata cgaccaatta cggagctaca atggtcgatt caggtagttt
    13921 gtacatcgaa aatgtgcttt cttattcgga tatattattg cctccatcgc ctttagatgc
    13981 ggtgattgat ttggtggatg tgaaacgcgt ttggattgca gacaaaaatc aatgtctggt
    14041 taaatcacaa aacgtcttta tcaatactcc tttctcggtt gaaggtccgc caacatctaa
    14101 tgtttttcgt ttagcatcat ccgcaggaaa agtttgggtt gctcccggag catgggatgt
    14161 agcctattca cctttgtatt cgattgatgg tgtttttgag tttgatggtt tgaattggaa
    14221 agactataaa cttaattatg gttctgattt cattcgggac atcgtttcaa ttgtaattga
    14281 tcccgcagat gcatcgcatg tttatgccgc ttcttggggt ggcggaattg ccgaaattaa
    14341 aaatggttca atcgttcagc aatatgcagc aactaatagt gggctaacgg gaatagccaa
    14401 ttatcccaac gatattcgtg tttctgatct tactatcgat aaagacggaa atctttggtc
    14461 aacttgttct tcttcttcta aacctcttgc cgttaaaacc cccggaggag aatggaaaaa
    14521 ctttgcattt aacaactcgg taaacaacca gtttctcggg aatatgttgg ttgatagctc
    14581 tggacttaaa tgggtgattg tacaagaacg cggtttgctc gttgcaaact tcgatggaac
    14641 gacgctagaa ggctataaat tcctaaatga tcaagttgga aatggtgcgc tcgcaagttc
    14701 ttctgtgctt tgcttagctc aggatttaga tggtcaaatt tgggtgggaa caactaaagg
    14761 aattagtgtc ttttatacgc ccgaagctat tttccaagaa ggttctactg gttgggattc
    14821 tcaaaccatc atcattagcc aagatggatt taaccaatat ctgctaaaag aagaagaggt
    14881 tacagctatt gcagttgatg gagcaaatcg taaatggatt ggcaccaaga aagccggaat
    14941 ctttgtcgtt tcgcccgatg gaacacaaca aattgccgag tttaactccg aaaacagtcc
    15001 gcttttaagt aataacgtaa caagcctttc cataaacggt gaatcagggg agatttttat
    15061 cggaacggat ctcggtattt gctcctaccg aacagatgcg agtagaggag gagataaatt
    15121 cggacacgtt tacacatttc ctaatcctgt tgaacatggc tacgaaggtc caattgctat
    15181 cactggatta gtaaccgatg ccgatgtaaa gattaccgat gtaagtggca accttgtttt
    15241 ttcaacggta gcaaacggcg gaactgccat ttggaatggg aaattatact caggacaacg
    15301 cgctgcaacg ggtgtgtatt tggtattctg caccaacgaa gacggtagcc aaaccaaagt
    15361 aaccaagttt cttttcatca actaagtatg ttacttgcca gtcaggctat tgtattacat
    15421 actttccgac atggcgattc atcggttatt gctcgaatgt acacgcgcga acgaggatta
    15481 gtttcatttc tcattcatgg agcaaagagc aaaaaaggag gaaacaaaac agctttattg
    15541 cagcctttga gtttgctcga agttcaaatg caactcaaag aaaaccgtgg cttgcagcaa
    15601 ttacgcgaga tgcggattga ttttccctat tcaggtattc ctttttcaac cggcaaaact
    15661 gcgcaggttc tttttttggc tgaagtgctt attcacacta ttaaggaaga agaacaaaac
    15721 gatcaactct tcgatttcct gcgcgatgcc ttgattctac tcgatcaaac accaagcgaa
    15781 cttcccgatt ttcatctgaa gtttttactc gaattttctc gctttctagg tttctatcca
    15841 gtagaaaatt actgcgaagc aacgccctat tttcagttta ccgatggcgt ttttcatcac
    15901 agtcccaatt caacttgttt cgatttacct accagcgcag ccctcaacca actcatgatt
    15961 acgggttttg gttccttgca aacgggattg tttaatcgcc ccatgcgcag acaagtttct
    16021 aggaaattac tcgagttttt tcgctggcat ttgccttcca tatccggact tcaaacgccc
    16081 gaagtactcg aagaagtttt cgggtagaag tattttcgat atcggtggat gggttgttca
    16141 aacccaacaa aactcaacca agcaagaatt aaagttgcac tcaaaatgag cagcggattc
    16201 atcacataca cttgccaagt tgattcggcg tagccagatg atttgagcag tggaatggca
    16261 aaggtgagca caatgccgtg gaagcaatat agtccaagcg aaagctgtcc gagataactc
    16321 atgcgcttga attttccgag tttaatggga gaatgaagtg caaagctctg ttcgaggatg
    16381 atgaaacaga agaacgcacc aaaaatgagc ttttccaaaa cgaccataac tggcgattga
    16441 aaccaacgga aatatccagc agttaagacg gcaagtagca gatatacgac aataatatgt
    16501 ctcttagata atcgctctat tacacgaatg aatcgtccat tatcaaatgc caattgcgca
    16561 cataatgccc ccaatccaaa attgcccaaa tagctgattg tattgaaata cagccttggt
    16621 tcgtctgaca aattcatcgc cctaaaaacg agtgaaacga ctatcattcc tccgctcacc
    16681 caaaggatgt ttcgatgcag aaaacgcaag acaaacgccc aaaaaacata gaattgttcc
    16741 tctacagcaa tcgaccaaaa gaacacgagt aaaaagagaa aattttgtcc gtgagcggca
    16801 atccaccagt tggaaacaaa taggaggaaa acttccacag gtggcagcgt gcttattccg
    16861 agtttatcac cccagaaata aacaaatccg tacgttccgc ccactaatac taagtacaaa
    16921 ggccataaac gcaagcctcg cctaaccatg aataaaccgg gctggaaact gcctgtttgc
    16981 tgccattctt tgagcgccaa ccaagtcagc aagaaactcg aaagcacaaa aaagtaatca
    17041 agtccgagaa atccagaacg gatatttaca gtagcgagat gatagccaaa tgattcttga
    17101 atagcaggat tcgtcgtaaa gaaacaatgc gccagaaaga catggaaaaa cccaagaaaa
    17161 cgcgtcgagt cgaggttttc aaagtagttc gattgtttgg cagtgctagt cattggcgtg
    17221 ttagcctttt cacaaatcgt tgaatggaag cttccggaga caaactagct ccgtcgagta
    17281 aaaaactgac aaattctttc gcctgaagcg gagccatcaa tacgcctttg gtgcctaaac
    17341 cattgaagat tcccaatgct tgatgtcgcg gatgaagacc gattagcgga cgacgatcga
    17401 gcaccgttgg acgaacacct gcttcatgca atacaacggt aaaaggaact tgaagaatgt
    17461 tgcgaagttt ctcttctaaa tccagtcgtt tatcttgagt cggatttggg tcggttgtgc
    17521 cccattcgta agttgatcca acccggaaca ttccatcacc aagcggaaca ccataaatgc
    17581 ttccattgaa aatttcatgt ttcaattcag gtgcataaat cacgagcact tcaccctttg
    17641 ctggattaaa tggaagatca ttaaaccacg gatttttcgt agctccaaca ccttcacaaa
    17701 aaatgatttt tggggcttct atatctttat aaatcactcc gtcgggagaa acttctaatg
    17761 cgtcataatc gaaaatttct tcacgtaaaa tgttcttttc caggaacttt cttctaaccg
    17821 ccgataacca agtgcgtgta tcgataaatc ctgctccggg aacatgagcg ccaccaaaag
    17881 tttgcttgag ccaaggcttt tcgtctgctg gtttggattc ctccaacatg ccaacaaact
    17941 caggatcttt gctcacatca atccaagatt ttctttccgc ttcacttccg tgtatgcgat
    18001 gtaacccgat tggataaaac aaccgaacgc cagtgaattc ttcctgttgg gcgtaaaaca
    18061 gatgggcttc ttttaaactt tcttttgctc tccaaccaag tgtaattcgt ttaaaaacta
    18121 ccgcattgaa taatcctgct gcaactcgcg atgaattgtg ctcatcgaaa acatctatca
    18181 ccaacacttc tgatcctctc gatattaatt ccagcgccaa tgtggtgccg gcaatacctt
    18241 gacctattat caaaaagtcg cacttcatgg cacgaagata aaccgataaa tgagtttttt
    18301 cgtttgaggt caatttcagg aacctgttta catttggccg tgcttcgact actctcatac
    18361 atactcttaa ttccctactg gatctggctg attctggtat ttctgtgttt tctggtttcc
    18421 tctagtctaa taggacttat ccttattcca accattggaa aacgagctca tcagccaatg
    18481 atgatggtgt ataaattttg gtcagcaaca ttcttctttt gtgccggaat tcgcattaaa
    18541 accatcaacc gcgaaaaact ttttggaaat ctaccttgca ttgtagtgag caatcatggc
    18601 tctaacctcg atatgttcat tggtacatac agtcttcctt taagtgtgaa acctttagcg
    18661 aaggttcaat tgaaaaaaat gcccttgctc gggtttttat ttgccacagt ttgcgtgctc
    18721 gttgatcgta gaagcaagga aagtcgcgat aaaagttcac gcgccatgat gagtgaaatt
    18781 ctaagcggaa attcgatttt tatctttcct gaaggaacgc gcaacaagag ccaagaacca
    18841 ctcaatgagt ttttcgatgg tgctttccgc tttgcgatag aaagtcaaaa accacttgtg
    18901 gcaatgtgca gcattaatgc tagaaacatt actccatcag ataattacgc tgttcgtccg
    18961 ggaacaatca ccatcaagtt tttaggtccg tacgaaacaa caggcttaac gaaagaggat
    19021 ttacctgctc tcaaaaccaa gattcgggaa gaaatgtatc aggttttgaa aaccgaagac
    19081 ccgatgttta aacacttgct ataaacggcc gcctaagacg aatgatttct tgattttcga
    19141 aacctgtcca tccaaagtga aagcatctaa cacgaaaatg taaatcccca atcgggcttt
    19201 ttcattgtcc tcgttgattc cattccaaga atactgtcct gaagttccca gtaaaattcc
    19261 tcgaccgatg tattttacta gtcttccgtt tgagtcgtaa atactcaatg ttccagtaaa
    19321 tccgggtgtg tcgaaagcat aagcgaaatt cacgacatcc tcataaccat caccatcagg
    19381 agaaaatact tcgggcgaaa tagttaccga accgggttga gaagtcacaa cttcactgta
    19441 ttgcgagttt cggtatgctg gagtagcaaa tcctgctgct gaagatgcag aattccaatt
    19501 agtaacatcc aatgccggac gatcataatt aacgcgttcc aaactcaccc catctaaatt
    19561 attcagcaaa gcaaaatgca tcgaggtgtt gtaattcatc cggtcaataa tggcattatc
    19621 actcacacga ctcaaggcaa gtgtgccatc atcattattc atggttggca ttgaactcat
    19681 ttgaataaat ccgcttggat tcgtggtcga ataaaagttt ttcacgcccg aaatatcatc
    19741 ggtcaacacc aaatattcac cagggaaaag caaataacca tcgggagcga tgaattctaa
    19801 atcgattgga acttgaacaa gcgtatcgta ttgactgagt tgaaggcttg ccagatcgat
    19861 gattttatta gaacgattgt agatttctac aaagtcactg gcaccatctt tttcgttact
    19921 gagcagttca ttgataacaa tgtcgtttgc ttgcggaatt tgaggaatag caaatcttcc
    19981 ggttgattga acatcgaaag catttccagc acaatcaaac aaatcactcg aaatacttat
    20041 cgtgtatagc acatccgttt gtaatgaggt agtcaaggca agttttaccg acttaaaatc
    20101 gggcgaatat gtttcgatgt aattgggttg accgattcct tgatcaatgg tgtatgaatt
    20161 caaatcctgt tgactgctca aacgaattga ttcggtaaaa aacactcgaa tggtatcgac
    20221 tgctattaca acaactcggt cgattttagg tgcttgttga tcactaacat tggaataaac
    20281 actatttatt ttccctggag aaccgcccca gttattggaa gaagcaatcc agttttcagc
    20341 tccagcacaa ggttgcatcg gactaatttg ttcgagcgaa tatccacctt cggacttaac
    20401 agtattttga taccatgaat ccgcataagt aacagacgaa atcacaactg tgtcaggatt
    20461 gtagagcgtt aatcttgctc cagtattggt gagagcagga aaggatgtta ttccaacaac
    20521 agctatcgaa tcgctgtaat attctaaacc atcagttcct gttagaacaa taaaactatc
    20581 gggttgaaga atacaagcag gaataacttt gcgattggtt cccgcttcaa tcatccagtt
    20641 ttctaggttg ataggaaaag ctgtccggtt agcaacttca ataaattcaa ccgatggcaa
    20701 acccgccgat ggatctggat cagccattat ctcattgata actacatcgt atggttttgg
    20761 gcgataatag ataaatggaa tctgctgagg attcatgtca tttcccgaca aatcctcaat
    20821 tgccgaaacc gttaactgat aggtgattcc ttcttgaaat gcagggataa atgttagggt
    20881 aacttttgaa aatgaagtca aggaacggaa tgcgctagaa ggctctccaa tacttgaatt
    20941 cactaaatag ttggctgttt cttcggcggt tgtttgagat acactttccg aaaagctaag
    21001 ttctagagca tgatccgaaa gcacctttat cgattgaaca actggcggaa tattgtctac
    21061 gatgattggt cctacataga aattatcggc aaagtacttt tgtcggtttg agctcgaata
    21121 ccgaactacc aaaccaaaac ttaacgaagt ggtataagtc acatcctgac cggttccaat
    21181 caactcatac aaagctccgg ttccgttgta atcggcgtat aatgaccagt ttccggcaac
    21241 atcgcgcgta acttttactt ttgttggatt ggtgctactg ctattgataa cacctgcaac
    21301 accagttata acgtacgatt caatgccagc accatcttta cgaaaaagag caacttcgtc
    21361 gggtgtgcct ccaatccgta caaaataacc ggtgttagcg cctaaaagat tagcatcact
    21421 tgaggtaaga aacacatcca tccgattgtt ggaggaagtt gaaactttcg gattaactaa
    21481 aaattcccat tgtgtattgt tgatagaagt ggaaggtgta ctaagataaa tctcgcgatt
    21541 cgaaagtgaa gtggtatcac cattactttg aagttgaaag gtgccatcta ccaaaaattc
    21601 actagcattt cccgaccaaa cggggttgga gctgaagttt ccatcagcaa aatcatcttg
    21661 cagctggcta tatccgcaat acgaaatcag gacaaaaaca atcgtaacca gttgtttaaa
    21721 catattttgc ataaaatgag gaaccctatt gtttactttt acggaattct attggttaaa
    21781 gaaacgaaac ttctactgta aatgaaagtc gctgttgttg gtgccaccgg actggttgga
    21841 aataaaatgc tacaagtctt acaggaacgt aattttccgg taacgaatct gattccagtt
    21901 gcatctgagc gctcagcagg gcggaggatt gcgtttcaac aagatgagtg gactgtcctc
    21961 actccagaaa atgccttatt gctacaaccc gacttagctt tgttttcggc tggcggaagt
    22021 acttctctcg aatgggcgcc acgatttgct gaaattggct gtaccgttat tgataattct
    22081 tctgcctggc ggatggatcc aagcaagaaa ttggtcgttc cagaagtcaa tgcacatgtg
    22141 ttagaagcaa gcgataagat tattgccaac cctaattgtt cgacgattca aatggtggtt
    22201 gcattgcagc cgcttcatca gaaatataaa atcaagcgca ttgtcgtttc gacctaccaa
    22261 agtgttaccg gtactggatt gaaagccgtg caacaactca tcaatgagcg taaaggaaca
    22321 ttgggagaac ctgcttatcc acacccaatc gacctaaata ttttacctca tatcgattca
    22381 tttctcgaca atggttacac caaggaagaa atgaaaatgc tcaacgaaac ccgtaaaatt
    22441 atgggcgacg atagtattcg agtgacagct acaaccgttc gtgttcctgt tattggcggg
    22501 cattccgaat cagtgaatgt ggagtttgag caggatttcg acttacaaca ggtgcgtgaa
    22561 ttattagctg ctgcaccagg tgttattgtc gaggatgatg tgctacacaa tcgttatccc
    22621 atgccaatca acgcgaataa tcgggatgag gtttttgtgg gaagattgag acgagatgag
    22681 agtcagccga atacgctcaa catgtggatt gtgtcggaca atctaagaaa aggagcagca
    22741 accaatgcgg tacagattgc tgaatacctg atgaataaag gttggctaac tgcctgatta
    22801 ggcttctaca acaggatctt cctttttctt ttcggtaaag aagtgctttt gataaaatag
    22861 gaaaatcact gcaccaatac tgatggagaa atcggccacg ttgaaaaccg gactgaagaa
    22921 gataaattcc tcgccagcat aaacaggtga ccaagaaggg aaatgtcctt caaacaatgg
    22981 gaagtagaac atatctacta ctcgcccatg tagaaaagta ccgtaagcac cttcggtggg
    23041 gaataaactt gctacttgtc caaagctatc gttgaagata agtccgtaaa atgcgctatc
    23101 aatgatgttt ccgatggctc cagcgatgat gagcgagaag gaaacttgaa gtctgggatg
    23161 aagttttttc tctttggaag ctttgagcaa ataatagcac atggttccaa ccaccacaat
    23221 tcggaaaagg ctcaggaaga gttttccaaa ttcacctccg aattccatcc caaatgccat
    23281 cccgttgttt tctacaaagg tgagatagca ccaatgacct gccacctgaa tcgactcgcc
    23341 gagcatcaga ttcgtcttga tcagaaactt aaccgtttgg tcaattgcta aaacagacag
    23401 taaaataatc agggatttct tcacgggaaa aggtcaaaaa aaagccccgc ggcgtgcgcg
    23461 gggccgaata ggttattgtt gatttttagc ttctatgcta agtgttgcat gcggtacaat
    23521 ccgcaaacgt tctttcggta ttaatttacc ggtaactcga caaatgccgt aggtgttgtt
    23581 ttgaattcga atgagagcag cttcaagatt ttcgatgaat ttcttctgac gagcagctaa
    23641 ttgaccggtt tcttcttttg aaagtacatc tgatccttct tccaataatt tgaaagtagg
    23701 tgatgtatca tcggttccgt gatcgtcttt attcgaaaat gcacttttaa gcaggtcgta
    23761 gtctttacgg gcttcgtcca gttttttctc aatgatttcc ttaaactcct gcaactcttc
    23821 cgggttataa cggttttttt cctgttggtt catagtccgt tcagtttaga gatggtgata
    23881 agcgtggata tttcgtccgt aagttctact aatgttcctc cggaagccgc gccattattg
    23941 acaatggtaa gagatgctcc aagggtttcg gcgcaaatat aggttttatt gttttcgatt
    24001 gctgcagtca atgcaggatg gttttgcaca atgatttcaa tagtgtcggt aacctctaag
    24061 ccgctgacct tgcgcaagtt ctgtatctta ttcacaagtt cgcgagccaa tccttcctca
    24121 accaagcttt ccgacaaggt cacatcaagc gcaactgtat acggtccttc gctcgctacc
    24181 aaccaacctg gaatatcttc ggtgaaaatc tccacatctt ctggactaag cacaatttct
    24241 tgtccttcga catcaaccgc caaacgacct tcgcgctgca attcattgat ttgcggcgtg
    24301 tttaaggtat ttacgacagc ggcaatgctc ttcatcaact taccgtattt aggtcctaat
    24361 gctttgaaat cgggtttgat ttttttctca atcactccgg catcatctcc tacatactcc
    24421 aattctttca cattggtttc ggataagatg agatctttaa cggcattcag ttgtgttttg
    24481 atatgtggat cgattaccgg caccagtatt ttttgcaaag gctgacgaac acggatgttt
    24541 accttcttgc gcaatcccaa tatcatagag ctatagcgct gagcgagccc cattcgttct
    24601 tctaaatccg aatcaataaa atccgaatta actgctggcc aagtactcaa atgaactgaa
    24661 accaacgatt gatttatttc atgcacatgc aagttgcggt ataaccattc tccaaagaaa
    24721 ggtgcaaccg gactaatcaa ctgcgaaaca acctccaagc attcgtacaa agtttcgtaa
    24781 gcagcttgct tatctgaatc gctctcgctt ctccaaaaac gacgacgacc taaacgaacg
    24841 taccagttac tcaattgctc tgtaacaaaa tcttcaatag cacgaacagc gggagttgga
    24901 tcgtaatcat ccatgcgttc tgctacttct tttttcaagc tatttaaacg agagataacc
    24961 caacgatcaa tttcaggacg atcagccaat ggaatctgct tagaagcatc cattttgaag
    25021 ccgtcgatat tggcgtaaag cgcgaaaaag gcataggtgt tataaagcgt tccgaagaac
    25081 ttacgctgaa cttcagtaat cccttctaca ttgaacttga gattgtccca aggttgtgca
    25141 ttgcctatca tgtaccagcg tgtggcatcg ggaccatatt ttcctaatgt ttcaaacgga
    25201 tcaacaccgt tccccaaacg cttcgacatt ttat
//
LOCUS       DAOOQW010000014        23193 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_2686_length_23193_cov_5.129398, whole genome shotgun sequence.
ACCESSION   DAOOQW010000014 DAOOQW010000000
VERSION     DAOOQW010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 23193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23193
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2686_length_23193_cov_5.129398"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..550)
                     /locus_tag="PK281_01870"
     CDS             complement(<1..550)
                     /locus_tag="PK281_01870"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HOY48115.1"
                     /translation="MAQTFSDWKNQTEQSYNEIKQIFGKINQNNPDQLLLKPNETSWN
                     AEECIQHLNSYGRYYLPELKSAITQIKLKNIKPEKNLKGSWIGRKFTKMMSVNSQGFP
                     ASSMKSPKDHRPISNTNSSKTLEEFESQLRELENIIQECNQINPDKARVPISISRFIK
                     LSAADVLDFYLAHHKRHLYQALR"
     gene            complement(962..1558)
                     /locus_tag="PK281_01875"
     CDS             complement(962..1558)
                     /locus_tag="PK281_01875"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOY48116.1"
                     /translation="MSISQLNPADLNSFKQFIFSIQPISDEAWNAFQSHWEVINLKRK
                     MLITRSGETERYIYFVLAGVQHAFYVTEEGKEVSIVFTYPYSFSGVADSFLTQTPSLY
                     SCETLSSSKLLRCEYNTFMELVEKFPEIKTLLLKSLSRVLKGALERQIELQCFNAEQK
                     FSTLLKRSPQVFQLIPHKYLASYLGMDAATFSKLLSKY"
     gene            1746..2756
                     /gene="mnmH"
                     /locus_tag="PK281_01880"
     CDS             1746..2756
                     /gene="mnmH"
                     /locus_tag="PK281_01880"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF008750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HOY48117.1"
                     /translation="MSVHIPLIDVRSPGEFKQGHIPNAINLPLFTDAERAIIGTLYKQ
                     NGHDAAVLEGLRIVGPKMAPLVEEATRIAPDKKLRVHCWRGGMRSNSVAWLFRQAGFK
                     VEVLAGGYKNYRQQVLSSLAKPLPLVVLSGSTGSGKTYILHELKNLGEQVIDLEGLAH
                     HKGSAFGALGQGQQPSIEQFENNLCVEIGQFDLNKPIWIEDEARKIGSVFIQDHLWNQ
                     MKQAPLFNLDIPISERLDFLVKEYGHFEKEALGASILKIQKRLGGQHVKACFEALELG
                     NLHFVAEVTLRYYDQTYAFGQSKRDPQKSIHFPCEKLDIIEIARSLKNYLQTHTLPIY
                     HD"
     gene            2749..3804
                     /gene="selD"
                     /locus_tag="PK281_01885"
     CDS             2749..3804
                     /gene="selD"
                     /locus_tag="PK281_01885"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015025200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HOY48118.1"
                     /translation="MIDSSNETIRLTQFAHGSGCGCKIAPSVLKDILQKHRNTADFPN
                     LLVGNAESDDAAVWNIGNDLAIISTTDFFMPIVDDAIDFGAIAATNAISDVYAMGGNP
                     FMALAILGWPVDKLPASLAAEVMQGAREVCEQAGIPIAGGHTIDSPEPFFGLAVSGQA
                     KLAHIRRNSTAKAGDILYLSKPLGLGIISTAARRGLAEQAHMHEAIQWMKTLNRCGAE
                     LAKLSGVHAMTDVTGFGLLGHLHEMTEGSDLTAHIRFDSVPRLACVSNYLARFIYPDM
                     TMKTFSWLKDSISELTGDQLLLLCDPQTSGGLLIAVDPAQQIQAEEILNQFQCPAKPI
                     GYLSERRLKSIFVSESV"
     gene            3801..4283
                     /locus_tag="PK281_01890"
     CDS             3801..4283
                     /locus_tag="PK281_01890"
                     /inference="COORDINATES: protein motif:HMM:NF019274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kazal-type serine protease inhibitor"
                     /protein_id="HOY48119.1"
                     /translation="MKKILVTCFLFLSFVLHAQVYNPNVCKDSTLVSNNPCYGVLYEP
                     VCGCDNVTYRNECLAYAQGLQNFTQGPCEFMDFDFTPNPASNDGQLKLKIICKGVSDI
                     NVWIFDTFFRQAYYQVSRQFSDIELYPDITGLGNGLFFIVVEANGVVKVKRLLINQAF
                     "
     gene            complement(4507..5808)
                     /locus_tag="PK281_01895"
     CDS             complement(4507..5808)
                     /locus_tag="PK281_01895"
                     /inference="COORDINATES: protein motif:HMM:NF016150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein"
                     /protein_id="HOY48120.1"
                     /translation="MQTQRIDILDSLRGMALLGILLMNILYFGLPHLIVESLEVRNEY
                     TGANYYTWWIVNGLFEGTMRATFSMLFGASCLLLLNGLESRSLPAPLTPADIHYRRLI
                     WLLLFGMINAFVFTWAGDILYTYAICGLFLFPFRNMKPRGLLFAAIVCMMFSLFNSGL
                     SSWDKTQMRQAGTEAQILIKEGKMLTKEQSEALEKWEGYVMESSTPHKKMESKEIQTD
                     MQKGYPVVFVRIAETNFELQTRAFYFDYIWDALTCFFLGMALFKWNVLTGKRSQKFYL
                     TLALTGYLIGIPASYYYLHLRLITHFDLTLIPSYFPLELYQFRRLFLAFGHIGLFIFL
                     FNSGLFKVIFKALGNVGRMAFTNYLMQSIFCSLIFFGYGFGLFGELERYQLYYVVLAI
                     WIFQILFSAIWLHYFRMGPFEWLWRSLTYWKKQPLKKDLVD"
     gene            complement(5840..6319)
                     /locus_tag="PK281_01900"
     CDS             complement(5840..6319)
                     /locus_tag="PK281_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48121.1"
                     /translation="MKHFSKFPLKIVAGIVGILLFTSCGDAIKKGDQLTAEDIRYIHD
                     MGILDDGEAILLFDSQGSLGGKRSSGNFITEKRIATYWIDDHDIKLNVVESAFYPDID
                     TIITKDLSSSLTYASYLEVHTKDKRVFKVNVDAKEAELQKFFDLANEKWRKNRGPRP"
     gene            complement(6492..6965)
                     /locus_tag="PK281_01905"
     CDS             complement(6492..6965)
                     /locus_tag="PK281_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48122.1"
                     /translation="MGIFRIQGQPKRKWYILVVILVLVGMGFIWLNKFNRESPKSSTG
                     DISDIEKYLIQEIQLNPRQVAELNRVREETDSLRTQLEKNIQVEQTRMEAEMKLKTHD
                     AQVIKHANTITAYEVLLNKLNREQTNKLELFLSKEQLDKLSGMTWELAHYFHPKK"
     gene            7344..8480
                     /locus_tag="PK281_01910"
     CDS             7344..8480
                     /locus_tag="PK281_01910"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOY48123.1"
                     /translation="MHYSCIVFQFEHVGKPGFGLKFYKDSVFYEAVRSHPLVRWMMDD
                     LFWWFPDNTESRQFIRGLFNDNPQERYAIRIGKISYSLPDWYGPIPLLQYLDVIGPET
                     RDELNKLSYWMQHRRYSRVSVSNYINTLQSFLRRYHVKNLSELEYETLIKYNREYIIE
                     RELSSSYQNTLASALKLLLGVVENRPFEPDQIERPRREHKLPNVLSKEEVKLILNALK
                     NSKHRLMLRIIYACGLRAGELLNLKPDDIMRDRELLHIKNAKGKKDRIVPIPSVLIAE
                     IDAYRKYQMGDVYLFSGQHKHEPYSSRSLQIIFKDAILRAGITRPATLHWLRHSYATH
                     LLERGTDIRYIQELLGHNSTKTTMIYTHVSKKALIEIRSPLEDL"
     gene            8591..9466
                     /locus_tag="PK281_01915"
     CDS             8591..9466
                     /locus_tag="PK281_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48124.1"
                     /translation="MNNRLNFRNLFCAFLLCFSFVFQVHGSNEEAIDIALWCKSGKLQ
                     LELVSLGGHQGECVQMRVKNRSAEFIQTRLEPGRKLVAEDSSAQDIFVVKSLVIRLAA
                     GEEKHFLANGFCCRASRHSPAKNMKFVLGEMMPSTWVQVAELIDKNNFPSSAIQAAVW
                     ALSDNHAVAAIQSDKPLVVESLRRLVAQVKGVELPWYSVAYEQDTTLLFSGRPQRVWG
                     EFTYYLPNQAMITMVIQDMRGRVMSVLTRSSAGMPGTYTYYLDQDVRKWKRGEYEFRI
                     LEDGANLNLKKKFIL"
     gene            9533..10054
                     /locus_tag="PK281_01920"
     CDS             9533..10054
                     /locus_tag="PK281_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48125.1"
                     /translation="MNSNIFGQSTWSRFMPDSYVILDSVSGYINEDAHKDWVVVLKIS
                     TETPDSSSLRPVLVFLSDEMGVLNLYARNDSVVLCADCGGMLGDPYLGVELDQQVISF
                     SFYGGANERWTRTIRFQYLPEKKEIVLKNDEGLDFWATDPDNQRAYTSNPEDFGKLLF
                     RDFSNSKAWESDE"
     gene            complement(10068..11285)
                     /locus_tag="PK281_01925"
     CDS             complement(10068..11285)
                     /locus_tag="PK281_01925"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOY48126.1"
                     /translation="MFEQEKQTARINLGISNPDQKIILSIDGGGMRGILTLQLLKRLE
                     QIAGSPCYKWVDFVSGTSTGAIISGLILAGKSANEIEELYKSLVTRVFKKRSITSNRT
                     LNPPEFDKVNYRDELRQLFTDETLEYLSQQSGIDCLFTSKDMAAGEEVFFTAIQNNGS
                     KGTYKSSLMRGVLEATMSAPTYFLPFERFIDGGTTTFNNPVLAAAMEAFYYDGRGKYT
                     PENTTIFSFGTGTCLRFVDPNKTANPDGIDAFFWLNYVMDESSKDASEMQVNMLRSGL
                     IPHLDFRRYQLSLDMNTLNKMPDKDISKHGIKNKEWLYQLTDKELSKIDMADVSKFGL
                     MEAIGEAAADFICPPAESQIPIANRSGNWFQKDLLAPNSKSRGALVSARGNIAQIQSH
                     LSNPAWIDSIKTE"
     gene            complement(11300..12181)
                     /locus_tag="PK281_01930"
     CDS             complement(11300..12181)
                     /locus_tag="PK281_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48127.1"
                     /translation="MDLTKTKQRSKKYLYITLGISVSLLWISLFLIGLLVNSDYYRVA
                     INYNFWDWEDVLGTFVSFTLSNVALLAFLSGLLGGITSILINTQGFRINREELLKNGI
                     HHSLIENPAISAIRGIIIFIGLLSIQYATSFTDLSAPKSEAQPAKNQLASISPGEVHE
                     LIIKIPKDSINKDEVAKFEKQLAAQLTNHSKDSNDIFIQEVQQLKFKHSQANSDVDRF
                     KLENDIEITRRKIHPPADTDFGGITLQSYFKFAIIASLLAFIMGYDPSQFNSFLNRLP
                     GNSSTAAKTGNPTNKTV"
     gene            complement(12196..13020)
                     /locus_tag="PK281_01935"
     CDS             complement(12196..13020)
                     /locus_tag="PK281_01935"
                     /inference="COORDINATES: protein motif:HMM:NF023288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramidase family protein"
                     /protein_id="HOY48128.1"
                     /translation="MTGVLKKGSKGEFVRLFQDMLHVLGNTIVSDGIFGSGTEKIVLQ
                     LQKDLGLKVDGLVGPKTWLLVEEKVRAKSNNSVLNTSQFLTESDFDAFATKYQVEKAA
                     VKAVQEVEAAGRGFVQGHIKLLFEGHIFWKELKNVGIQPSTIQAGNENILYTKYFTPN
                     PYYRENQYDRLNKAIRIQEEAAYRSASYGLFQIMGFHAQDMGFDSAKKMVDYLSQSEA
                     NQLDVFGKFAEKNNLLSFIRNHDWAGFAKRYNGSAYKTNKYDEKLAKAYTRYSIIT"
     gene            13258..17001
                     /locus_tag="PK281_01940"
     CDS             13258..17001
                     /locus_tag="PK281_01940"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HOY48129.1"
                     /translation="MNKISVILLLVLFFSCSSETKKQPADYGPKVTDAIGKVIPAESI
                     EPPKIIVLDESKLKHIPIGKPIAYPLANNVIPVGKPTIVPLTLPIEFIPGKDSLEAPE
                     ITLVKDKPFVPKKRMPVTALPLRTKDGARYNFNYLSEDQGMNSSLVLSILEDKKGNIW
                     IGTDGGGVSKYDGKSFTHFTEKEGLASNAVFDILEDRKGNLWFGTRTGGVTKYDGKYF
                     SNYSLQEGLGNNEVYSIYEDKRGDIWFGLKGGITRYNGKSFIYYSKKQGISPGYYFSF
                     IEDKKGILWFGNSGEGIYSFDGKVFTHITSKCGLPQDRVLALLQDRKGDFWIGTHGSG
                     ICRFDGKQFYVYSEKEGLVNKYVNSLTEDKNGNIWIGTNYGISVYDGQSFTNYTDKDG
                     LSDGQVRTILEDETGNFWIGTNDGGLIKYFGSSFTNYIDSTLECKHGIRSIVEDKKGQ
                     FWVGTNTQGLLQFDDKYVRKYTQKEGLKSNYVSSLLEDSYGNLWIGTFGQGVCKFDGK
                     SFTYFTTEEGLSGNNIVSLAEDRSGNLWIGTYSGGVTKYDGNRVEELLNGKQFSKNEI
                     RDIQVINGKPVKSFFHFTEKQGLGGDIVWSIFQDKQSNMWFGTFESGVTQYDGKKFKR
                     YTIDPNTTKNTVWSIYEDNKENLWFGTFGAGAIRFDGNRIDALEKGKKIPPQELTDLR
                     KENGKYVKSFLYLTDKEGLSDNFVRSIYQDKQQNLWLTTNKALHLIKEKKRSDAQLPK
                     TINSPQQFIESSQLVLFNKEDGMKAGGFFQGSVMLDSKNRFWMTSSNSLSTLNLNTFN
                     IDKVVPELQLETILIDEKFIDYLLLKSDSAHIQELPEQFENIKFSDVSNFHNYPLELE
                     LPYNLNHLTFNFIGIDWSAPHNLKYQFKLEGLDQDWSQLSTSNEADYRNIPFGKYTFK
                     IRAIGNSDKWSKTLEYTFVIHPPWWRTTWAYILYIIATILIILLIVKWNSRKLRARAK
                     ELGVKIDEAVHEIKEQKQLIEEKHKEIKDSINYAERIQRSLLASKDLLNKNLNDYFIY
                     YQPKDVVSGDFYWAAELNNGDFVIICGDSTGHGVPGAIMSILNIACLREAYLQGITSP
                     DLILNETRRLVIENLKNDGSEEGGKDGMDASLICFNPSKDRLTFCGANNPAWILRNQE
                     WIELKPDKMPIGKHDKDSISFTLKEMELIKGDIIYLFTDGMPDQFGGPLGKKYKYNQL
                     KQFLLSIQHLSMDEQHQEIKTELNNWKGDLEQVDDILVIGLRI"
     gene            17153..17473
                     /locus_tag="PK281_01945"
     CDS             17153..17473
                     /locus_tag="PK281_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007839701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOY48130.1"
                     /translation="MNLRRDVFQAISDPTRRAILLLLTAQSMTAGAIASNFDTARPTV
                     SKHLQILTECELLKQEQQGREIYYHFNPLKMKEIADFIEPFRKMWDDKFNKLETVMRK
                     YKNK"
     gene            17475..17939
                     /locus_tag="PK281_01950"
     CDS             17475..17939
                     /locus_tag="PK281_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HOY48131.1"
                     /translation="MDMKTQIQAEEGKQELFITREFDLPLDLLFKAYSEPEFIEQWMG
                     TKVIKHENKNHGSFQFETSHNGHVVFKANGTIHQFELNERIIRTFEMENMPMGVQLEF
                     LEFEKLSDDSSKLTIQIIYRSEKHRAEQLKLPFAFGISKAHDRLQEITNNLK"
     gene            17946..18329
                     /locus_tag="PK281_01955"
     CDS             17946..18329
                     /locus_tag="PK281_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007811728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HOY48132.1"
                     /translation="MRNKIIYWVATALLSFGMLGSGISQLFHLKDMNEIITHIGYPLY
                     FMYIIGVWKILGVIAILIPNFKLLKEWAYAGFFFLMTGALFSHLFMGDVGNAILGPLF
                     QTLFIVTSWYFRPANRKLNSLSFNQ"
     gene            complement(18406..19920)
                     /locus_tag="PK281_01960"
     CDS             complement(18406..19920)
                     /locus_tag="PK281_01960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HOY48133.1"
                     /translation="MKILSTAQIRACDKYTISKQQISSTELMERAATSCYDWIIQHTH
                     PDSSYVLLIGPGNNGGDAWVIARMLLEAGKKVNAFALPAESLSDDNQINKTRFEALGG
                     TVYELNSAALESIQAGSVLIDGIFGSGLNRPLEKSTALIISELNSISAIKIALDAPSG
                     FFTDSPMPDKAEAFRAHHTLCFQSPRLMYLLPGSEVYTGTWHVLDIGLEMPNDEAFQQ
                     DAFNKFEYTLLPDIENLFNAPNVFAHKGTNGHALLAGASHGKTGAIVLAAHACLRSGV
                     GLCSVYIPGEAYSILQTALPEAMVFTSEELTHLSGSFNPEAFDAIAFGPGAGKHDDTA
                     RVLKNLIQSVQTPLLIDADGLNLLAENPTWLAFLPKGTVLTPHPGELDRLCGKVTNAY
                     DRLMNARELSRKTGAVIVLKGAFTAVCSHSGDVFFNSTGNPGMAKGGSGDVLSGIITA
                     LLARGYSPVQAARAGVFIHGLAGDIARENLGYTAMHASDIINSLPQAWKCVEKG"
     gene            complement(19950..20534)
                     /gene="coaE"
                     /locus_tag="PK281_01965"
     CDS             complement(19950..20534)
                     /gene="coaE"
                     /locus_tag="PK281_01965"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HOY48134.1"
                     /translation="MKRIGLTGGIGSGKTTVARIFKLLDIPVYNSDEQARILTDSDPD
                     IKTAILNQFGAEVFSSDGKLNRAALANIVFNNPDSLHSLNAIIHPAVAHDFEKWCSQQ
                     NAAFIIKEAAIIFEHQLEKHLDGVIVVEAPDDLRIKRVMKRNASTEAEVRARMQQQFP
                     QENLVRMADWVIHNDEKQLLIPQVLHIYSQILAG"
     gene            complement(20621..21601)
                     /locus_tag="PK281_01970"
     CDS             complement(20621..21601)
                     /locus_tag="PK281_01970"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HOY48135.1"
                     /translation="MARTKTTKVLSSVINRRKVTVFGICLLISALFWVLLAFNGHYST
                     RIQVPVTYINMPESRVLMEKLPHKVDIQISGTGYQLLSYILQPEQATVLLDGRSIGMS
                     PASQAGEAFLTTFQGIDFFNREHGDIKALNILPDTIRFSFFDRGFRKVPVHLNSYLDF
                     ERQFSLSDSIQLSPDSITITGPVQVLDSIKQIETEALVLSKLNKSGNYTVKIKRPSDR
                     VTYAPLEVGARLEVDKFTEAVLEVPVYVDHLLSSDSLDIFPQSVKVRYLVSLRDYNKV
                     NPKLFRIAADAFDLRNAQATNLRLYLRKVPPYVRQVQMSPETVEFIIRKK"
     gene            complement(21608..21922)
                     /gene="yajC"
                     /locus_tag="PK281_01975"
     CDS             complement(21608..21922)
                     /gene="yajC"
                     /locus_tag="PK281_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020211591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HOY48136.1"
                     /translation="MYNALLLMGAPGQSGLGSILPMLLIIVVFYFFMIRPQMKKAKEA
                     KTFRESLKKGDTVVTIGGIHGRITEVNETTFILEAESGAKFRVEKSAVSNSPNDQLIA
                     GK"
     gene            complement(21946..22413)
                     /locus_tag="PK281_01980"
     CDS             complement(21946..22413)
                     /locus_tag="PK281_01980"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HOY48137.1"
                     /translation="MNRVRLSIFAACATLVSFTACDQKSDGKVTADMVNNSASADGEL
                     NIPVSAIKFDQDTFNFGTVVEGEKVAHAYTFTNTGKNDLVITKAIGSCGCTVPEWPKE
                     PIRPGKTGKIDVVFNSDRRVGKANKTITVYANTEPATNLLLLTGNVKGDPSEE"
     gene            complement(22545..>23193)
                     /locus_tag="PK281_01985"
     CDS             complement(22545..>23193)
                     /locus_tag="PK281_01985"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY48138.1"
                     /translation="TADGRNAQVDDITPAGAGMYVLGMSLNGCQNRFDTLYATVNPKP
                     IISIHGDTIQTPNGSGVLYASGTDGLFYYWNFYGSQSLLVNEIYTADRDTVVAFWNNY
                     EGFIRAEVMAEDTNGCMSDAVSLSIHVTNAEGISSIQKTSGIEVFPNPANEKLFLQNK
                     SEVKIPAFISDANGRILLQFKLQASSTQSIPVENLASGMYILHAGTSHLPLIIQH"
BASE COUNT         7234 a         4122 c         4556 g         7281 t
ORIGIN      
        1 ctcttaatgc ttgatagaga tgtcgcttgt gatgtgctaa ataaaaatcc aatacatctg
       61 cagcacttaa tttaataaat cgagaaatag aaataggtac acgggcttta tcgggattaa
      121 tttggttaca ttcctgaatg atattttcta attcacggag ctgactttca aattcctcta
      181 gggttttcga actgtttgta ttagaaatcg gtcgatgatc ttttggcgat ttcattgaac
      241 tagcaggaaa cccttgcgaa ttgaccgaca tcattttagt gaattttcga ccaatccagc
      301 taccttttaa gtttttctcc ggttttatat tttttagctt gatttgagtg atagcagatt
      361 tcagttcagg taaataatag cgtccatagc tattgagatg ttggatacat tcttcagcat
      421 tccagcttgt ttcatttggt tttagcaaaa gttgatctgg gttattttga ttgattttac
      481 caaatatttg cttaatctca ttataggatt gctctgtttg atttttccaa tcggagaatg
      541 tttgtgccat aggttgaatg tatggtgcaa aaatctaaat caaaaaccga ataaatcttg
      601 gtgtaggtca agaattttgg agattcgaaa gaattagtag attagaaata gtcatttttt
      661 ggagtgaata ggttatatat taaatttaca ccggtctgta tgccttgatt atcgatcttt
      721 tcatctcacg cgcacgcggg gccgaccact aaccacccac cggctgaaag aaacaaaatt
      781 cccgagacct gattcgtctg ttaaacgttt atgaaacggc tagtttttaa cagcattgtt
      841 aataaaatta aagcatagca tttcagcatc gagtaatcag ctcaatgagt aatcttaatt
      901 cgtataattc tcaaatagtc gaatctaatt ccaacctaat tttgagttaa ttattttcga
      961 tttaatattt cgataaaagt ttgctaaaag tagctgcatc catgcctaga taagaagcca
     1021 agtatttatg aggaatgagc tgaaatactt gaggtgatcg ttttaataaa gtgctgaatt
     1081 tttgttccgc attaaaacac tgcaattcta tttgtcgctc taatgctcct tttaatactc
     1141 tacttaatga ttttaataaa agtgttttta tttcggggaa tttttctacc agttccataa
     1201 atgtattgta ttcgcatcgt aatagtttac tgctactaag ggtttcacaa ctatataaag
     1261 atggagtttg tgttaaaaat gaatctgcaa ctcccgaaaa tgaataagga taagtaaata
     1321 cgatactcac ttcttttcct tcctctgtta cataaaaggc atgttgtaca cctgcgagaa
     1381 caaaatatat ataacgttcc gtttcaccag aacgagtaat caacatttta cgttttaaat
     1441 tgattacttc ccaatgcgat tgaaatgcat tccatgcttc atcactgatc ggttgtattg
     1501 aaaaaataaa ttgtttgaaa ctgtttaaat cagcaggatt taattgtgaa atactcattg
     1561 aaaattttga aattatgtgc agcacaagtt aagttataca agcattcttc attcattttt
     1621 tgatattaat tatttagtaa ttacatatgt gaatcggtat cttttgctat tcaatagtat
     1681 ccttaaattt tacttgaatg ttttttgaat gttcaatcat ccaatttagc ttactatctt
     1741 gcagcatgtc cgtgcacata ccattaatag acgttcgctc acccggggag tttaaacaag
     1801 ggcatatccc caatgcgatt aatttgccgc tttttactga tgctgaacgt gctataatcg
     1861 gtacactata taagcaaaac ggtcacgatg cagctgtttt agaaggtttg cgaattgtcg
     1921 gacctaaaat ggctccattg gtagaagaag ctactcgaat tgcgcccgat aaaaaattac
     1981 gagttcattg ttggcgtggc ggaatgagaa gcaatagtgt cgcttggtta tttcgtcagg
     2041 ctggatttaa ggtggaagtt ttagctggag gatataaaaa ctatcgtcag caggtacttt
     2101 catcattggc taaaccattg ccattagtgg tattgagcgg ttctacagga agtggtaaaa
     2161 cctatatttt acacgaatta aaaaacttgg gtgagcaggt aattgatttg gaaggattgg
     2221 cgcatcataa gggctctgct tttggtgcat taggacaagg acaacagcct agcatagaac
     2281 aattcgagaa caatctatgt gtagaaattg gtcaatttga tttgaataaa ccaatttgga
     2341 tagaagatga agcccgtaaa ataggttcag tatttattca agatcattta tggaatcaaa
     2401 tgaagcaagc tcctttgttc aacttagata ttccaatttc tgaaaggctc gattttcttg
     2461 ttaaagagta cggtcatttt gagaaagaag cattaggagc atcgattctt aaaatccaga
     2521 aaaggctagg aggacagcac gtaaaagctt gttttgaagc actggagttg ggcaatttgc
     2581 attttgtggc agaagttact cttcgctatt atgatcaaac atatgctttt ggacaaagca
     2641 aacgagatcc ccagaaatcg attcattttc cctgtgaaaa attggatatt attgaaattg
     2701 cgcgttcatt aaaaaactat ttgcaaaccc atactttgcc tatttatcat gattgattca
     2761 agcaacgaaa ccattcgtct tacgcaattt gctcatggtt caggctgtgg ttgtaaaata
     2821 gcacccagcg tgttaaaaga tattctgcaa aagcatcgaa atactgccga tttcccaaat
     2881 ttattagtgg gtaatgccga aagcgacgat gccgctgttt ggaatattgg gaacgattta
     2941 gctattattt cgaccaccga ttttttcatg cccattgttg atgatgccat cgattttggt
     3001 gctattgccg cgactaatgc cattagcgac gtatatgcca tgggtggaaa tccttttatg
     3061 gcattagcaa tcttaggttg gccagttgac aaacttcctg caagtcttgc tgcggaggta
     3121 atgcaagggg ctcgtgaggt ttgtgaacaa gctggaattc ctatcgccgg aggacatacg
     3181 attgattctc ctgaaccatt ttttggatta gcagttagcg gacaggctaa attggcacat
     3241 attcgacgaa atagcaccgc aaaagcaggc gatattttat acttgagcaa acctttaggg
     3301 ttgggcatta ttagtacagc agctcgtcgt ggtttagcag agcaagcaca tatgcacgaa
     3361 gcaattcagt ggatgaaaac cttgaatcgc tgtggagcag aattagccaa gctttcgggt
     3421 gttcacgcca tgaccgatgt gacaggtttt ggcttgctcg gacacttaca tgaaatgacc
     3481 gaaggaagcg atttaactgc tcacatacga tttgattctg tgccacgttt agcatgtgtc
     3541 tccaattatt tagctcgttt catctatccc gatatgacca tgaaaacttt ctcctggttg
     3601 aaggattcca tttccgaatt aacaggcgat caattgttac ttctctgcga tcctcaaaca
     3661 tctggaggtc ttctcatagc ggtagatcca gcgcaacaaa ttcaagcgga agaaatttta
     3721 aatcaatttc aatgtcctgc aaaaccaata gggtacttgt cggaacgccg tcttaagtct
     3781 atatttgtga gtgaaagcgt atgaaaaaaa tacttgttac ctgttttcta tttctaagtt
     3841 ttgtcctaca tgctcaggta tacaatccta atgtgtgtaa ggacagcact cttgtatcca
     3901 acaatccttg ttacggggtt ttatatgaac ccgtttgtgg ctgcgataat gttacctatc
     3961 gaaatgagtg tttagcctat gcacaaggtt tgcagaactt cacacaaggc ccatgtgagt
     4021 tcatggattt cgatttcaca cccaatccag cttccaatga cggacaactt aaattgaaaa
     4081 ttatttgcaa aggagtaagc gatattaatg tgtggatttt cgatactttt ttccggcaag
     4141 cttattatca agtctctcga caattttctg atatcgagct ttatccagat attactggct
     4201 taggtaatgg tttgtttttt attgtggtag aggcaaatgg ggttgtaaaa gtcaaacgat
     4261 tattaatcaa tcaagcattt taatttacag ggcggcatgc aggctataga cttccaatct
     4321 ctttaatagc tgaagcttta gcgaaagcta ttatccttgc cgcttacgtt aaggagttaa
     4381 atagcacatt taaactacac tctgctaatc ttaatttgat tgagatattt aggtaaatgt
     4441 atctttcggg taaatattta acggtgttga aatacatcca ttcctattca atctagattt
     4501 aagaattcaa tcaactaaat cctttttcaa aggttgtttt ttccaatagg ttaaacttct
     4561 ccaaagccac tcaaatggtc ccattcggaa ataatgaagc cagattgcac taaatagaat
     4621 ttggaaaatc caaatagcta gaaccacata gtaaagctgg tatctttcca attctccaaa
     4681 taacccaaag ccgtatccaa agaagattaa actgcaaaaa atactttgca tgaggtaatt
     4741 agtaaatgcc attcttccga catttcctaa agctttaaaa atcactttaa ataatccaga
     4801 attaaataga aaaatgaata atcctatatg cccaaatgca agaaataatc ttcgaaactg
     4861 atacaattcc aaaggaaaat aggagggaat taatgtcaga tcaaaatggg taattaatcg
     4921 taaatgtaaa taatagtagc tagcaggaat tccaatcaaa tagccggtta atgctagtgt
     4981 caaatagaat ttctgacttc gttttcccgt caaaacattc catttaaata aagccattcc
     5041 gaggaaaaaa caggttaaag catcccagat ataatcgaaa taaaaagcac gtgtttgtaa
     5101 ttcaaaatta gtttctgcaa ttcgaacaaa aacaaccgga tatccttttt gcatatctgt
     5161 ttgaatttct ttcgattcca tttttttatg tggagtgctc gattccatta catatccttc
     5221 ccatttttct aaagcttccg actgctcttt tgtgagcatt tttccttcct ttattagaat
     5281 ttgtgcctca gttccggctt gtcgcatctg tgttttatcc cagctgctaa gtcccgaatt
     5341 aaataatgaa aacatcatac acacgatcgc tgcgaatagg agccctcttg gtttcatgtt
     5401 tctaaaagga aataaaaaca aaccacaaat ggcatacgta tataaaatat cacctgccca
     5461 ggtaaataca aaggcattaa tcatcccaaa taatagcaac caaatcaacc ttcgataatg
     5521 aatatcggct ggtgttaatg gagcgggtaa actacgagat tctaatccat tcagtaataa
     5581 caagcaactt gctccaaata acatcgaaaa tgtagctcgc atggtacctt caaacaaacc
     5641 attaactatc caccatgtat agtagtttgc tccggtatat tcatttcgta cttctaaact
     5701 ctcaacaatt aaatgaggta gaccgaaata cagaatattc atcaacagaa tacctaaaag
     5761 agccatccca cgtaatgaat ctaggatatc aatacgttgt gtttgcatcg acagtatgta
     5821 ttaaaatgga ttgttcagtt taaggtcgtg gacctctatt cttacgccac ttttcatttg
     5881 cgaggtcaaa gaacttctgt aattcagctt ctttggcatc aacattcact ttaaaaacgc
     5941 gcttatcttt tgtatgaact tccaaataag aagcatacgt gagcgaacta gataaatctt
     6001 tggtaattat tgtatcgata tcgggataaa aagcactttc aaccacgttg agtttaatgt
     6061 cgtggtcatc aatccaataa gtagcaattc ttttttcggt gatgaaatta cctgatgaac
     6121 gttttcctcc taatgaacct tgagaatcga aaagcaaaat tgcttcccca tcatccaaaa
     6181 tacccatatc gtggatgtat cgaatgtctt ctgctgttaa ttgatcgcct tttttaatcg
     6241 catcgccaca acttgtaaat agcagaatac caacaattcc agccacaatc ttgaggggaa
     6301 attttgaaaa gtgtttcatg gattcgagga aataagttgt taaggcaggt tcttcaaata
     6361 tagaaagcta attccaatac atacataggt tttctgaatt attattttac caataagctg
     6421 aatagaaata aatcaaggaa gaaatcgaaa ttcgatctgc acaattaaaa tactttcttc
     6481 atgtagtgtt cttatttttt gggatgaaaa taatgcgcta attcccatgt cattccgctc
     6541 agtttgtcca attgttcttt tgaaagaaat agctcaagct tattcgtttg ttcgcggttg
     6601 agtttgttta gaagtacttc gtatgcggta atagtattcg catgtttaat tacctgagca
     6661 tcatgtgttt ttaatttcat ttcagcttcc atgcgagttt gttccacctg aatgtttttt
     6721 tcaagctgag ttcttaagga atccgtttct tccctaactc gattcaattc cgccacttgc
     6781 ctaggattta attgtatttc ctgaatcaaa tatttttcga tatcagaaat atctccggta
     6841 gatgatttag gagattccct gttaaacttg tttaaccaaa taaatcccat tccaacgagc
     6901 acaagaataa ccacaaggat gtaccatttg cgtttaggtt gaccttgtat cctgaagatt
     6961 cccatgtggg cggttaatcg tgatgttggg ttcttgatga attaatatac aaaacaagtt
     7021 cttaaaccga atggttttgc gctataccga ctttatttat ccaaatggat attcacaaag
     7081 aaccagccta taattctctt gatttcagct ctgcttaaca actctatact ctctccaatt
     7141 aaagttgaac aattagtgaa tgatggcata tgcattttta ggaaatcatc agagaatcaa
     7201 tcccgagtac atagataatt ttgtcaacac agttgtttaa aagtaaatct gccacatttc
     7261 tttgggatga gttgggtcct tgttattctg ttttaaattt gtgattcgtt ccggtatatg
     7321 cttaatctca tttttcatat accatgcatt attcctgtat tgtttttcaa ttcgaacatg
     7381 ttggcaaacc cggattcgga ctgaaatttt ataaagattc tgttttttac gaagctgttc
     7441 gttctcatcc attagttcgc tggatgatgg atgatttatt ttggtggttt cctgataaca
     7501 cagaaagtcg ccaattcatt cgtggcttat ttaacgataa tccgcaagaa cgctatgcta
     7561 ttcgtattgg taaaatatcc tactcattac ccgattggta tggtccaatt cccttattac
     7621 aatacttgga tgtaattggt cctgaaactc gcgatgaact caacaaactc agttactgga
     7681 tgcaacacag gcgatacagt cgtgtgtcgg tgagtaatta catcaatacg ttgcaaagtt
     7741 tcctccgtcg ttatcacgtt aaaaatctat ccgagctcga atatgaaaca cttataaaat
     7801 ataaccgcga atacatcatc gaacgagaac tttcttcttc ctatcaaaac acacttgcta
     7861 gtgccttaaa actcttactg ggtgtggttg aaaatcgacc ctttgaaccc gatcaaattg
     7921 aacgtcctcg tagggaacat aaattaccca atgttttatc gaaagaagaa gtcaaactca
     7981 ttctcaacgc tcttaaaaac agtaaacacc gactcatgtt gcgcatcata tacgcatgtg
     8041 gtttacgggc gggtgaatta cttaatttaa aacccgatga cattatgcgt gatcgggaat
     8101 tactgcacat aaaaaatgct aaaggaaaga aagatcgaat tgtcccgatt ccttccgttt
     8161 taattgcaga aatcgacgcc tatcgcaagt atcaaatggg tgatgtgtat ttattttcgg
     8221 gtcaacacaa gcacgaacca tatagtagta gaagtctgca aatcattttt aaagacgcta
     8281 ttttacgagc tggaatcact cgcccagcta ctcttcactg gcttaggcat tcctatgcaa
     8341 cgcatttact cgaacgcggt accgatatac gatacattca agaattactg gggcacaaca
     8401 gcactaaaac aaccatgatt tacacgcatg ttagtaagaa agcactcatt gaaatacgaa
     8461 gtccattgga agatctttaa tcattgattg gcaatgaaat cgagaatact cacctcaaat
     8521 tcgattgatt ttactagaca ataaaagcac gaaaaattgg gttatctata ttcaatcagc
     8581 ttttatgggt atgaacaata gattgaattt tcggaattta ttttgtgcat ttctactctg
     8641 tttttctttt gtatttcaag tacatggatc taatgaggaa gcaatagata tagcattatg
     8701 gtgcaaaagt ggaaagcttc aattagagtt agtttcattg ggaggacatc aaggcgaatg
     8761 tgtccaaatg cgggtgaaaa atcgtagtgc tgaattcatc caaacacgat tggaacccgg
     8821 acgaaaatta gttgcggaag attcttcagc gcaggatatt tttgttgtaa aatcgcttgt
     8881 aattcgatta gcagcaggtg aggaaaagca ctttttagct aacggatttt gttgtcgggc
     8941 aagtagacat tctccagcaa agaatatgaa atttgtttta ggtgaaatga tgccatctac
     9001 ttgggtacaa gtagctgaat taatcgacaa aaataatttc ccttcaagtg ccattcaagc
     9061 ggctgtatgg gcattgtctg ataatcatgc agtggcggca attcagtccg acaaaccttt
     9121 agttgttgaa tcgttgagaa gacttgtggc acaggtgaaa ggagtagaat tgccttggta
     9181 ttcggtagct tatgagcaag atacaacgct attgttttcg gggcgtcctc aacgtgtttg
     9241 gggagagttt acgtattatt tacccaatca agctatgata actatggtga ttcaagatat
     9301 gcgaggtaga gtaatgtctg tgttgactcg cagttcagca ggtatgccgg gcacttacac
     9361 gtattattta gatcaagatg tacgcaaatg gaagcgggga gaatatgaat tccgaatttt
     9421 agaagatggt gcaaatttga acctcaagaa gaagtttatt ttgtagattc attctttatt
     9481 aatcagcaaa attggaatgg ttaagttttg gatactggta gtttttttgt tcttgaattc
     9541 caacattttc ggtcaatcta catggtctcg ctttatgcca gattcctatg tgattttaga
     9601 ttcggtgagt ggatatatca atgaagatgc tcacaaagat tgggtggttg tgttaaaaat
     9661 atctaccgaa acacccgatt cctcctcgct acgacctgtt ttggtattct taagtgatga
     9721 aatgggtgtg ttaaatttat acgctcgtaa cgacagtgta gttttgtgtg ccgactgtgg
     9781 tggcatgtta ggagatccgt atttaggggt ggaattagat caacaggtaa tttcgttttc
     9841 tttttatgga ggagcaaacg agcgatggac gcgcacgatc cgttttcaat atttaccaga
     9901 gaaaaaggaa attgtattga agaatgatga aggcttagat ttttgggcaa ccgatcccga
     9961 taatcaacga gcgtatacta gcaatcccga agattttgga aaattattat tccgtgattt
    10021 ttcgaatagc aaagcttggg aatcggatga gtaaatttag aagagtttta ctctgttttg
    10081 atggaatcaa tccaagctgg attcgataaa tgactttgaa tttgggcaat attaccgcga
    10141 gccgaaacaa gtgccccgcg cgatttggaa ttaggtgcta ataaatcttt ttgaaaccaa
    10201 tttccgctac gattggcaat aggaatttgt gattcagcag gtggacatat aaaatcggct
    10261 gctgcttcac ctatagcttc catgagtccg aatttgctta catcagccat atctattttg
    10321 gaaagttctt tatctgttaa ttgatacaac cattctttgt tttttattcc atgtttactg
    10381 atgtccttat ccggcatttt attcagcgta ttcatatcga gcgatagctg atatcttcta
    10441 aaatcgagat gaggaatcaa gccacttcga agcatgttta cctgcatttc actagcatct
    10501 ttactcgatt catccataac ataattgagc cagaaaaatg catcaatacc atcaggattg
    10561 gctgttttat ttggatcaac gaagcgaaga caggtaccag taccaaagct gaaaatagtc
    10621 gtgttttcgg gcgtatattt tcctctccca tcgtaataaa atgcttccat cgcagccgct
    10681 aaaactggat tgttaaaggt tgtagtacca ccatcgatga accgttcgaa aggcaagaaa
    10741 tacgtaggcg ccgacatggt tgcctctaaa acaccccgca ttaaagaact tttgtaggtt
    10801 ccttttgaac cgttgttttg gatagctgta aagaatactt cttctccggc agccatatct
    10861 ttggaagtga ataaacagtc gatacccgat tgctgagaaa gatattctaa ggtttcatcg
    10921 gtgaatagtt gtcttaattc atcccgataa tttactttat caaattcggg aggatttaag
    10981 gtcctgttcg atgttattga tcgtttctta aatacccgag tgactaatga tttatatagt
    11041 tcttcaattt cgttcgccga tttacctgcg agaattaatc ccgaaataat agcacctgtg
    11101 gacgtgcccg aaacaaaatc aacccattta tagcaaggac ttccggcaat ctgctccaat
    11161 cgttttagta attgcagggt aagtattcca cgcattccac caccatcaat gctgagtatt
    11221 attttttggt caggattgct aattcctaaa ttgatgcgtg ccgtttgttt ttcttgttcg
    11281 aacatgaaac tcaattatat taaactgttt tattggttgg attgcctgtt ttagctgctg
    11341 ttgagctatt tcctggtaaa cggtttaaga agctgttaaa ctggcttgga tcatagccca
    11401 tgataaaagc caataaagat gcaataatgg caaatttaaa atacgattgt aaagttatgc
    11461 ctccaaaatc tgtgtcagct ggtggatgaa ttttacgacg ggttatttcg atatcatttt
    11521 caagtttaaa acgatcaaca tctgaattgg cttgtgaatg cttgaatttt aattgttgaa
    11581 cctcttggat aaatatgtcg ttcgaatctt ttgaatgatt tgttaattga gcagctaatt
    11641 gcttttcgaa ttttgcaact tcatctttat tgattgaatc tttaggtatt ttaataatta
    11701 attcatgcac ttcaccagga ctgatagaag ctagttgatt tttagccggt tgagcttccg
    11761 atttgggtgc agataaatcg gtaaatgaag tagcatattg aatggataat aatccaataa
    11821 aaataatgat tccacgaata gcagaaatag ctggattttc gattaatgaa tgatgaattc
    11881 catttttcag aagttcttct ctatttattc taaatccttg tgtatttatt aatatactgg
    11941 tgattcctcc aagtagtccc gacaaaaaag cgagtaatgc aacgttactt aaagtaaagg
    12001 aaacgaatgt tccaagtaca tcttcccagt cccagaaatt ataattaatt gctactcgat
    12061 agtaatcaga atttaccaat aaacctatca gaaataagga aatccataat aaggaaacgc
    12121 ttattcctaa agtgatatac aggtattttt tacttctttg tttggtttta gttaaatcca
    12181 tgataattta ttaaactaag taatgataga atatcgagtg taagcctttg ctaatttttc
    12241 gtcgtattta ttggttttat atgcactacc gttataacgt tttgcaaagc ctgcccagtc
    12301 gtggttgcga atgaaactta ataagttgtt tttctctgcg aattttccga atacatccag
    12361 ttgattagct tccgattggg atagataatc taccattttc ttagccgaat caaatcccat
    12421 atcctgcgca tgaaatccca taatttgaaa taaaccatac gaagccgaac gataagccgc
    12481 ttcttcctga atacgaatag ctttatttaa acgatcatat tggttttctc ggtaataagg
    12541 attgggagta aagtatttag tataaaggat attctcattt ccagcttgta ttgtcgaagg
    12601 ttgaatgcca acatttttta attctttcca aaagatatgt ccttcgaata ataatttaat
    12661 atgtccttgt acaaaccctc gtccggcagc ttctacttct tgtactgcct taacagcagc
    12721 cttctcaact tgatatttag tcgcaaatgc atcaaagtcg gattcagtca aaaactgact
    12781 ggtgtttaat accgaattat tcgatttagc tctgactttc tcttccacta ataaccaggt
    12841 ttttggacca actaaaccat ctactttgag acctaaatct ttttgtagct ggagtacaat
    12901 tttctctgtt ccactaccaa agattccatc cgaaacaatg gtattaccaa gtacatgaag
    12961 catatcctga aataaacgga caaactcacc tttactacct ttttttaata cgcctgtcat
    13021 ttttcatttt attatagctg atttataaat ggatttaact atttacagaa caacttatta
    13081 gacagaacaa tactaattaa attcaggaat taagtttagg tgaaaatgct ggaaggagat
    13141 aggggtagtt tacacgggta gtttagtttg tttgtcaagc taattgaaaa aatctatttt
    13201 tgaaattcaa ggtatatgag aaattgacaa aacacacata attcaactaa ttaattcgtg
    13261 aataaaatta gtgtaatcct actgcttgta ctcttttttt cgtgcagttc ggaaacaaag
    13321 aagcagcctg ctgattatgg gcctaaggtt acagatgcaa taggaaaagt aataccagct
    13381 gaatcaatcg aaccaccaaa gattatcgtg ctcgatgaga gcaagttgaa gcatataccc
    13441 attggaaagc ccatagcata tccattagct aacaatgtaa ttccagtggg taagcccact
    13501 attgtgccat taactttgcc aattgaattt attccaggta aggattcctt agaagctcct
    13561 gagattactt tggtgaagga taagccattt gtcccaaaaa aacgaatgcc agttactgct
    13621 ttgccattac gcacaaaaga tggagcacgg tataatttta attatctaag tgaagatcag
    13681 ggtatgaatt cttcactggt tttatccatt cttgaagata aaaagggaaa catatggatt
    13741 gggacagatg gaggaggagt aagcaaatat gatggtaaat cgtttactca ttttaccgag
    13801 aaagaaggtt tagctagcaa tgctgttttt gacattttag aagatagaaa aggtaatctt
    13861 tggtttggga ctagaacagg aggtgtgaca aaatatgatg ggaagtattt ttccaattat
    13921 tcgcttcagg aaggattggg taacaacgaa gtatattcaa tttatgagga taagcgaggg
    13981 gatatctggt ttggactgaa aggtgggatt acacgatata atggaaaatc atttatatat
    14041 tattcgaaaa aacaaggaat ttctcctgga tattatttct cttttataga agataaaaaa
    14101 ggaattctct ggtttggcaa ttctggagaa ggaatctatt cattcgatgg aaaggtattt
    14161 actcatatca cctcaaaatg cggactccct caagatcgag tcttggcttt acttcaagat
    14221 cgaaaaggag atttttggat tggtactcat ggaagtggta tctgcagatt cgatggaaaa
    14281 caattttatg tttattctga aaaagaagga ttggtcaaca agtatgtgaa ctcattgaca
    14341 gaagataaaa acggcaatat ctggattgga acaaattatg ggataagtgt ttacgatggt
    14401 caatccttta caaattatac ggataaagat ggattgagcg atggtcaggt tagaacaatt
    14461 ttagaagatg aaacaggcaa tttttggatt ggaacgaatg acggtggcct aattaaatat
    14521 tttggtagtt cttttaccaa ttacattgat agtacacttg aatgtaaaca tggaattagg
    14581 agtatagtag aagataaaaa aggacaattt tgggtaggca caaatacaca aggtctgctt
    14641 caatttgatg ataaatatgt taggaaatac acacaaaaag agggtttaaa aagcaattat
    14701 gtttcttctt tattggagga ttcatatggt aatttatgga taggtacgtt tggacaagga
    14761 gtttgtaaat tcgatggtaa atcattcacc tatttcacta cagaggaagg acttagcggc
    14821 aataatattg tttcgcttgc tgaagatcgt agtggtaatt tgtggattgg aacttatagt
    14881 ggaggcgtta ccaaatacga tggaaaccgt gtggaagagt tgttaaatgg aaagcaattt
    14941 tcaaaaaatg aaattcgaga tattcaagta ataaatggta agccagttaa atcgtttttt
    15001 catttcacag agaaacaagg attaggtgga gatattgttt ggtctatatt ccaagataag
    15061 cagagtaata tgtggtttgg caccttcgaa agcggtgtta ctcagtatga tggtaaaaaa
    15121 ttcaaacgct ataccattga tccaaataca acaaaaaata cggtttggtc aatatatgaa
    15181 gataataaag aaaatctgtg gtttggaacc tttggtgcag gagccattcg gttcgatggt
    15241 aatcgaattg atgcattgga gaaaggtaaa aaaataccac cacaggaatt aactgacctt
    15301 cgaaaagaga atggaaaata tgttaaatca tttctctatt taacagataa agaaggctta
    15361 agcgacaatt tcgtacgatc tatttatcaa gataaacagc agaatttatg gctaaccact
    15421 aacaaagcgc tccatctaat taaggagaag aaaagaagcg atgctcaatt acctaaaacc
    15481 attaattcac cacaacaatt tattgagagt agccaattag ttctctttaa taaagaagat
    15541 gggatgaaag cgggaggttt ttttcagggt tctgttatgt tggatagtaa aaaccgattt
    15601 tggatgacaa gttccaattc attaagtact ttgaatttga acacatttaa catcgataag
    15661 gttgttcccg aattgcaatt agaaacaata ttaatcgatg aaaaatttat tgattatcta
    15721 cttttaaaat ccgatagcgc tcacatacag gaactgccag agcaatttga aaatattaaa
    15781 ttctctgatg tatccaattt tcataattac ccattggaat tagaattgcc ttataattta
    15841 aatcacttaa catttaattt tattggcatt gattggtctg cacctcataa tctcaaatac
    15901 cagtttaaac tggaaggatt agatcaggat tggagtcaat tatctaccag caatgaagct
    15961 gattatagaa atattccatt tgggaaatac acctttaaaa tacgtgcaat tggtaattcg
    16021 gataaatgga gtaaaacatt agaatacacg tttgttattc atcctccatg gtggagaact
    16081 acttgggcat atattttata catcatagca acaattttaa ttatcctgtt aattgtaaaa
    16141 tggaatagca gaaaattacg agcaagagca aaggaactcg gagtaaaaat cgatgaggca
    16201 gttcatgaaa taaaagaaca aaagcaatta attgaagaaa aacataagga aataaaagat
    16261 agtatcaatt atgctgaacg aatccaacgc tccttactcg ctagtaaaga tttactcaat
    16321 aaaaacctaa atgactattt catttactat caacccaaag acgtggtaag tggcgatttt
    16381 tattgggctg cagaattaaa taatggtgat tttgtaatca tttgtggcga tagtacagga
    16441 catggagttc cgggtgcaat tatgagcatt ttaaatattg cctgtttaag agaagcctat
    16501 ttgcaaggca taacaagtcc cgatttaatc ttaaatgaga cccgaagatt agttattgaa
    16561 aatctgaaga atgatggtag cgaagaaggg ggaaaagatg gtatggatgc cagtttaata
    16621 tgttttaatc ctagtaaaga ccgacttaca ttttgcggtg ctaataatcc ggcgtggatt
    16681 ttacgcaatc aggaatggat tgaactaaag cctgataaaa tgcctatcgg aaagcacgat
    16741 aaagattcca tcagctttac tttaaaagaa atggaattaa ttaaagggga tattatttac
    16801 ctatttacag atggaatgcc cgatcaattt ggtggaccac taggtaaaaa atataaatac
    16861 aatcaattaa aacagttttt actttccatt cagcatttga gtatggatga acaacatcag
    16921 gaaattaaaa cggagctaaa caactggaaa ggtgatttag aacaagtcga tgatattttg
    16981 gtcattggtt taaggattta aattttgcct agttgaaaat aaatccttca ctatttattg
    17041 gttaggattt cataagctag attttcttgc cacaaaaatt caagaagaaa tatctataaa
    17101 aatttgcgta gttaaataac tacatatata tttgtagtca aatagctaca taatgaattt
    17161 gagacgagac gtatttcaag cgatatccga tccaacacgg agagcaattc ttttattact
    17221 tactgctcag tcaatgactg caggtgcaat tgcatccaat tttgatacag caaggccaac
    17281 agtttcaaaa cacttgcaaa tattgactga atgtgaactc ctcaagcagg aacaacaagg
    17341 cagggagatt tattatcatt tcaatccttt aaaaatgaag gaaatagctg actttattga
    17401 gccatttcgc aaaatgtggg atgataagtt taataaactt gaaacagtaa tgagaaaata
    17461 taaaaataaa taaaatggat atgaaaacac aaatacaagc agaagaaggg aagcaagaat
    17521 tatttattac gagagaattt gacttgccat tggatttgtt attcaaagct tattctgaac
    17581 ctgaattcat tgaacaatgg atgggaacaa aggttataaa acacgagaat aaaaatcatg
    17641 gtagttttca atttgaaact tctcacaatg gacatgttgt ttttaaagca aatggaacaa
    17701 tccaccaatt tgaattaaat gaaagaataa ttcgaacgtt tgaaatggag aatatgccca
    17761 tgggtgttca actcgaattt ttggaatttg aaaaactcag tgatgattca agtaaactga
    17821 ccattcaaat tatttatagg tcagaaaagc accgagcaga acaactcaaa ttaccttttg
    17881 cttttggaat aagtaaagct catgacagac ttcaagaaat aacaaacaat ttaaaataga
    17941 atgatatgag aaacaaaata atttattggg ttgctacagc cttattatca tttggaatgt
    18001 tgggcagtgg aatttctcaa ctatttcacc tcaaagatat gaatgagatc attactcata
    18061 ttggctatcc actctatttt atgtacatta ttggtgtgtg gaaaatattg ggtgtaattg
    18121 ctattttaat cccaaacttt aaattattaa aagaatgggc gtatgcaggt tttttctttt
    18181 taatgactgg agcattattt tctcacttat ttatgggaga tgttggaaat gctattttag
    18241 gtcctttatt ccaaacacta tttattgtaa catcttggta tttcaggcct gcaaacagaa
    18301 aattaaattc actttcattt aatcaataag ttgaaacctg gagaagattt tttctagtct
    18361 aggtttaaac tcacattgtc gcatttaact ctatatcgtt tcaaactaac ctttctccac
    18421 acacttccaa gcttgtggaa gactgtttat gatgtcgctg gcgtgcatgg cggtataacc
    18481 caggttttcg cgggcaatgt cgccggctag tccgtgaatg aaaacacctg ctcgggctgc
    18541 ttgtacgggt gaatacccac gtgcgagtaa tgcggtaata attccgctga gcacatcgcc
    18601 acttccacct tttgccatac cgggatttcc ggtgctattg aaaaatacat cacccgaatg
    18661 actacatact gcagtaaatg cacctttaag cacgattaca gcacctgttt tacgactcaa
    18721 ctcgcgtgca ttcatcaaac gatcatacgc gtttgtaacc ttgccacata aacgatcgag
    18781 ttcgccggga tgtggcgtta atacggtgcc tttgggcaaa aaagctaacc aagttggatt
    18841 ttcggctaac agatttaatc catcggcatc gatgagtaat ggtgtttgta ctgattgaat
    18901 gaggttttta agaacgcgtg ctgtatcgtc gtgcttgcca gcaccaggac caaaggcaat
    18961 agcatcaaat gcttcgggat tgaaggaacc actgagatga gttaattctt cggaagtaaa
    19021 aaccatggct tcgggtaaag ccgtttgaag aatggaatag gcttcaccgg gaatgtacac
    19081 actgcacaat cccacaccac tgcgcaaaca ggcatgtgca gctaatacga ttgctcccgt
    19141 ttttccatgt gatgctcctg ctaacaatgc atgtccattt gtgcctttgt gtgcaaaaac
    19201 attgggagca ttaaatagat tttcgatatc gggcaaaagc gtgtattcga acttattgaa
    19261 cgcatcttgc tgaaacgctt catcgttggg catttccaaa ccaatatcga gcacatgcca
    19321 agttccggtg tacacttccg aaccgggaag taaatacatc aaacgcggag actgaaaaca
    19381 aagcgtatga tgagctctaa acgcttcggc cttatctggc atgggcgagt cggtgaaaaa
    19441 gccgctagga gcatctaggg cgattttaat ggcggaaatg ctattgagtt cagagatgat
    19501 tagtgcggtt gatttttcta atggacgatt taatccactt ccaaaaattc catcaatcaa
    19561 taccgaaccg gcttgtatgc tttctagtgc ggcagaattt aattcgtaaa cagttccacc
    19621 taaagcttca aatcgtgttt tattaatttg attatcgtca ctcaaacttt cagcaggtag
    19681 agcaaatgca ttgacctttt ttcccgcttc taacagcatt ctcgcaatca cccaagcgtc
    19741 gccaccattg tttcccggtc caattaaaag tacataagat gaatcggggt gtgtatgttg
    19801 gataatccag tcgtaacagc ttgttgctgc tcgttccatg agttcggttg aactaatttg
    19861 ctgcttggaa atagtgtatt tatcgcaggc gcggatttga gcagttgaca agattttcat
    19921 gtcgcaaaga tggtcaaata gcaatcgatt tatccggcaa gaatttgcga atatatgtgc
    19981 aagacttgcg ggatgagtag ctgtttttcg tcgttatgaa taacccaatc ggccatgcga
    20041 accaggtttt cttgcggaaa ttgttgttgc atgcgggcac gaacttcggc ttcggtacta
    20101 gcattccgtt tcatcacccg ttttattctt aaatcatcgg gagcttcaac tacgattact
    20161 ccatcgaggt gtttttcgag ctgatgttca aaaataatag cggcttcttt gatgataaat
    20221 gcagcatttt gctgtgagca ccacttctcg aaatcatgcg ctacggctgg atgtataatc
    20281 gcattgagtg aatgaagtga atcgggatta ttgaatacaa tattcgccag tgcagcacga
    20341 tttagctttc cgtcggagga aaaaacttct gctccaaatt gattgaggat agcagttttg
    20401 atgtcgggat cagaatcggt gaggattcga gcttgttcat cggaattgta cacaggaata
    20461 tcgagcagct taaagatgcg ggcaacggtt gtttttccgc taccaattcc gccagttagc
    20521 ccgatgcgtt tcatgaggtg aaggtacaac ggaaatgaat attccgattc aatcgaagcg
    20581 aatgcaccga atcattaata atccagaaaa gtaactaaaa ttatttttta cggatgatga
    20641 attcgaccgt ttcgggactc atttgtacct gtcggacgta aggtggcact ttgcgcaaat
    20701 acaagcgtag gttggtggct tgtgcatttc ttaaatcgaa tgcatcggca gctatacgga
    20761 acaattttgg attcacctta ttgtaatcac gaagtgaaac caagtaacga actttaaccg
    20821 attgcggaaa tatatcgaga ctgtcgctac tgagcaggtg atccacataa acaggaactt
    20881 ctagaaccgc ttcggtgaat ttatccactt cgagacgagc gcccacttcg agcggagcat
    20941 aggtaacacg atcgcttggt cgtttgattt ttaccgtgta attccccgat ttattgagtt
    21001 tgcttaatac aagcgcttcg gtttcgattt gtttgatgga atcgagcact tgaactggac
    21061 cggtgatggt aatactatct ggactaagct gaatgctatc actcaggctg aattggcgtt
    21121 cgaagtcaag ataggaattg aggtgcacag gtacttttcg gaacccacga tcaaaaaagg
    21181 aaaagcgaat ggtatcgggc aagatgttga gtgctttgat atcgccatgt tcccgattga
    21241 agaaatcgat tccctgaaaa gttgtgagga aagcttcacc cgcttgagaa gccggactca
    21301 tgccaataga tcttccatcg agcaagacag tagcttgttc gggttggagg atgtaggaca
    21361 aaagttgata cccggttcca ctaatctgta tatcgacctt atgcggcagc ttctccatca
    21421 gaacgcgact ttcgggcatg ttgatgtagg taaccggcac ttggatgcga gtagaataat
    21481 gtccgttaaa tgcaagcagc acccagaata gggcagaaat cagcaagcag ataccgaaaa
    21541 cggtaacctt ccgacggtta atgaccgaac tcagcacctt ggtggttttg gtccgggcca
    21601 tgggaaatta ttttccagcg ataagctgat cgttcgggct attagaaaca gccgatttct
    21661 ccacacggaa tttcgctcca ctttcggctt cgaggatgaa cgtagtttcg ttcacttcgg
    21721 taatacggcc atggattcca ccaatggtaa caacggtatc accttttttc aggctctcac
    21781 gaaaggtttt agcttcttta gcttttttca tttgtgggcg aatcatgaaa aagtagaata
    21841 ccacaatgat aagcaacata ggaaggatag aacctaaacc gctttgacct ggagctccca
    21901 ttaggagcaa tgcattatac atatcgtttg taattgaaat gagaattatt cttctgaagg
    21961 atcacctttt acattaccag taagcaacaa caagttcgtt gcaggttctg tattcgcgta
    22021 aactgtgatg gttttatttg ctttcccaac ccgacgatcg ctgttgaaaa cgacatcaat
    22081 ttttcctgtt ttacctgggc gaattggttc ttttggccat tctggaacgg tacaaccaca
    22141 actaccaatt gctttggtaa tgactagatc gtttttccct gtattcgtga atgtgtaggc
    22201 atgtgctact ttttcgcctt ctaccactgt tccaaaattg aaggtgtctt gatcaaattt
    22261 tatagccgaa accgggatgt ttaattcacc atcggccgaa gctgaattgt tgaccatgtc
    22321 ggcagttact ttaccgtcgg acttttggtc acaagctgta aaacttacta aggttgcaca
    22381 agctgcaaaa atgcttaatc gtacgcgatt cattcgttta ttatttcggg aggcaaaact
    22441 acataaaaat gagcggcaga aaaattaaaa gcggctcaat agaaatgtaa catccaagag
    22501 cttagcttgt tttaaatcgc ctgaatttca gcgttatgtt ggtattaatg ctgaatgata
    22561 aggggaagat ggcttgttcc ggcgtgaaga atatacatgc ctgaagctag gttttcaact
    22621 ggaatagatt gagttgagga tgcttgcagc ttgaattgaa gcagaattcg tccattagca
    22681 tcgcttatga atgctgggat tttcacttcc gatttgtttt ggaggaataa tttttcattt
    22741 gccggattcg ggaaaacctc aattccagaa gttttttgga tgcttgaaat tccttctgca
    22801 ttggtgacgt gtatggataa agaaacagca tcgctcatgc agccgttagt gtcttcagcc
    22861 atgacttcgg cacgaataaa tccttcgtaa ttattccaga aagctaccac cgtatcgcga
    22921 tcggctgtgt agatttcgtt taccaataaa gattgacttc cgtaaaagtt ccagtagtaa
    22981 aacaaaccat cagtcccgct agcatagaga actcctgagc cattcggtgt ttggatagta
    23041 tcgccgtgaa tgctaattat tggtttagga ttaaccgttg cgtaaagtgt atcaaatctg
    23101 ttttgacaac catttaaact catcccaagc acatacattc cggcaccagc aggtgtaata
    23161 tcatcaacct gagcatttct tccatcggct gta
//
LOCUS       DAOOQW010000015        23123 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_2697_length_23123_cov_5.400208, whole genome shotgun sequence.
ACCESSION   DAOOQW010000015 DAOOQW010000000
VERSION     DAOOQW010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 23123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23123
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2697_length_23123_cov_5.400208"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1077
                     /locus_tag="PK281_01990"
     CDS             <1..1077
                     /locus_tag="PK281_01990"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOY48139.1"
                     /translation="RLLIISGPNAGGKSITLKTIGLLQMMLQSGMLVPVGEQSEMGYF
                     QRLFADIGDSQSIEYELSTYSSRLAKMKYILEFADKKTLVLIDEFGTGTDPELGGAVA
                     EAMLEELSRRRTLGVITTHYMNIKLAADRLDGVRNACMLFDDETLQPMYQLVIGQPGS
                     SYTYIIAEKTGLPVALINRARNMTSKDKVTLDRMLQQLQKEQQELRKLLHVLDEKQAI
                     ADKSKSKYDELHEKMKLKMESKKATQEESGKLIELGKKLQSITKEWEAAKDKKPIFAK
                     LTKILSAEKRKKIDKKQQLKKEKVKAQLLEKKKAEIKVGSKVKLLSGRQTGIVEEIQN
                     NRARVIFGNLKTLASLETLEVVED"
     gene            complement(1074..3419)
                     /locus_tag="PK281_01995"
     CDS             complement(1074..3419)
                     /locus_tag="PK281_01995"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOY48140.1"
                     /translation="MKYPLILIALIAFLLPFTSSAQSCIQIESVLVDACDDGFTNPEG
                     LNEMFRFRNGNFDLNISDITILDGWPSESVNALPFNGFVQNSSTQAKTVELNNTIISC
                     GYLLEPPNGIIPQNRKVLAITSFDVSASLNSFANLSDTLYIIYHQHTGEGGGHFLNQN
                     TGAPQDQTLRIQITGANTCYEEVTYQRALLVNTNGQNVAQNGATVEFSDDGTASYTNT
                     GCTAPIEPFSADWVNPGPFCGNSTAVDLNTFVTGSSSGTWSGTGVTGSIFNPVGLNGS
                     YSVTYSVAPPSGCITQPASLTLNIIVSESVDASFTNPQLLCATLGSIDLDTLVTGTTG
                     GTWSGNGVTGSQLNLNGLDGNIQITYSVGTGTCSDILSQTLQVVSLSPINITGDTLYC
                     NGETPNALSINADPNATVSWYSDPALTTFLSNGNSITPANGQNAGYYAVQSEQGCYSD
                     TTGVTVEFSVVAIPTGDTLLTFCEADPIPLASVQSSTSPITWYSDESLSAVIATGLNY
                     QSSIENDTLYVTATSGACESEALEIIIQQIGLLTAEIIPPSETSLCSGQPIELTSAAN
                     NQNSWNTGSTASSITVTSAGTYTLTRSGQCNTATDQIIITGIPVTANFTTDVDSGYVI
                     LPVNVTDQSVNGETYSWTLNGEPLIFTAPGTLTFPDSGTFVLELVVSNSTGCYDSDSA
                     VIKVLSDKLLLVVPNVFTPNSDGFNDLFQVKYNAVKTFQARVFNRWGKQLYSWENVST
                     GWDGLHNSEKVPDGTYFFIISGTDIKDQPFEEKGTVTLLGN"
     gene            complement(3613..6366)
                     /locus_tag="PK281_02000"
     CDS             complement(3613..6366)
                     /locus_tag="PK281_02000"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOY48141.1"
                     /translation="MKKFLLSFLFLSLCLANTHIQAQSNCIDVMSILVNSCAPNGNGE
                     GKNEMVRMRTGVLDLNIYDMNLTWPNTNLSWNGIVMDAGTAQTTAEFNATILSCGYLI
                     EPLDGIIPAHHDVLLISSYNCNTSTNFFSQLGDTLYIVYANENTNTGHVLNYVPNANP
                     NNQTLYVDFPTIGGCTEQVTYYRTQLITTAGNIGDEDGATVNFNIQGDATYINIGCVA
                     PITPFSANWTAPNVCANSQPFNLNLLITGTTGGTWSGPGVTGNIFDPTGLSGSVDITY
                     TVGAGSCSQSNTQTVAIHGAVSPNWTAPPLICSNAGTVDLTQYVSGTAGGTWSGQGVN
                     GNNLIPTSLTGSITLTYTVGVSGCTQTESHSIQIQPINVNWVNPGTICSGNGTINLND
                     QLPNGVVGTWTGQNITDSIFDPTGLTGQVIITFSQDINGCSGSNTQPVTVAVAPDASW
                     TNPGFICDLSTLINLDGLITGDLGGTWSGDGVTGSTFDPSLVVDSTVIRYGVSGNGCV
                     STVIQTIYIGTIPVLEVNGSDLFCSGGELTPFDVTALPGATISWFSDSQLTIQIGTGA
                     TYTPSGTGDQTVYVVQSLGDCTSEVSSFSYTIVPTPNSPITDANVNVCEGTAIPVLTA
                     TSTSNVYWFSDVDMQNLVQQGPTYQPTQTGITLWVIARENGCVSEPVVVQVNGLPLAT
                     VAISVTGPSTICHGSSVALQATSNISFVWSTGAQTNDLIVTQAGTYSVSASNSCNSAT
                     DQIVVLDLSPDASFDVSTLEGYAPLNLAVVPASSTGCDWYLNGSSTIISQNALLLENA
                     GTYTLKQVCDNNGCMDSTSRVIVVSNGSFVLELPNSFTPNGDGYNDIFKAKSSGIVEF
                     RSTIFDRWGQEIYTWEGAGNSWDGTKKGNPVSDGVYFYVIKGKDFQSNDFERYGSITL
                     VRN"
     gene            complement(6450..8048)
                     /gene="bshC"
                     /locus_tag="PK281_02005"
     CDS             complement(6450..8048)
                     /gene="bshC"
                     /locus_tag="PK281_02005"
                     /inference="COORDINATES: protein motif:HMM:NF021586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HOY48142.1"
                     /translation="MKTRCSYVPYRKTGKFQPIVCDYLDQDPFLKPFYEAAPTLDNFQ
                     HFIHQKSQKTVDRVVLTEVLRDQYKQAGILDSIVEEQISKLLSEHTFTITTGQQTGIL
                     LGPLYSPLKIISAIKMSQELSNKYPQNQFVPIFWMATEDHDVAEIDHVWVEGQKLQWN
                     SNQTGPVGRFSLDGMEEVLVQFDQIAGKSPDAVRLKEIFKNAYALPNLSLATRYIVHQ
                     LFGHWGLLVIDADDTRLKKAFAPIIIQDILEKNSFREVSQTKEKLEERYHSQVNGREI
                     NFFFLEDGYRERIVEEEGRFLTHDGKHSWNSEELKGLISEHPDKFSPNVLMRPLYQET
                     ILPNIAYFGGGAEISYWLELKGIFDHYRMPFPALMLRNSALIIDRRNTLRIEHARFQE
                     EDLFKTRSELEKQIALSLSESDLELKNEFSDLTRLINQLKNHAQGFDTTLVSSAEALG
                     KRLEDQLGRYSQKLIRAQKRASAIEINRLNEVFNHIYPGGTLQERKESISTFILRHSF
                     LILDVIMDGCQPLEKSFLLIYQED"
     gene            complement(8045..9361)
                     /gene="rimO"
                     /locus_tag="PK281_02010"
     CDS             complement(8045..9361)
                     /gene="rimO"
                     /locus_tag="PK281_02010"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006264477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HOY48143.1"
                     /translation="MKTKTLKKNKVNVVTLGCSKNIYDSEVLMGQLKANKFEVEHESK
                     QDDSEIVIINTCGFIDNAKEESINTILRYAEAKANGQVDKVYVTGCLSERYKPELEAE
                     IPEVDAWFGTRDLPRLLKTLKADYKHELVGERLLTTPAHYAYFKISEGCDRPCSFCAI
                     PLMRGGHVSIPMEQLEAQAKQLVKNGTKELLLIAQDSTYYGLDLYKDRKLAELMSRLS
                     DINGLEWIRLHYAFPSGFPTDVLDVMRERENICKYIDMPLQHISDSMLKSMRRGTNKQ
                     KTIDLVNMIRDKVPGMAIRTSLIAGYPGETEKDHEEMLQWIQDSRFERLGVFTYSHEE
                     NTHAYSLVDDVPADVKQERADAVMDVQMGISASLNQEKVGNTYKVLFDRKEGPYFIGR
                     TEFDSPEVDNEVLVEAKDDSYVRVGDFAQVKIDRAEEFDLFGTFVK"
     gene            complement(9478..10536)
                     /locus_tag="PK281_02015"
     CDS             complement(9478..10536)
                     /locus_tag="PK281_02015"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY48144.1"
                     /translation="MKHPILFLSTLFASLQLVAQPTMTFPSMPSVGYSLSTSSVELGS
                     ANEGAAGANVMWDFSMLADTGDVTSLTFFDASAISQSSSFPAANIALGAQTGGNNFYT
                     FLSANSNNVQQQGTFVSSTFGDINMIYTDPQIQYQFPATFNSTVSDPFRYEVALDIGI
                     GSTFTSTTGTSSYVVDAYGTLFNWSGTYTNVLRFKHRTLSVDTAYSSFFGQTDTSYTE
                     SRSTMYEWLSVIEGGTIPIFSITYDTTISDGTPTYSVNASHSYNNDNTGIQKQSSDII
                     QLFPNPTSGKINVSIQPNVEKILVSDLSGRVVSTMQTTKSNATTIQEFDLSAIQPGVY
                     YLQLQSPNGITVKKFIRN"
     gene            complement(10619..11572)
                     /gene="ftsY"
                     /locus_tag="PK281_02020"
     CDS             complement(10619..11572)
                     /gene="ftsY"
                     /locus_tag="PK281_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013997707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HOY48145.1"
                     /translation="MAFFGLFSREKKETLDQGLAKTKESFFSKLAKVLIGKSKVDEEV
                     LDNLEEILIGADVGVSTTLKIIERIEARVGRDKYTTSSELNGILKEEIAALLEENRSS
                     DLVDFDLPKTDGPYVIMVVGVNGVGKTTTIAKLAYQFKEAGKSVVLGAADTFRAAAVD
                     QITIWADRVGVPLVQHGMNADPAAVAYDAVQMGKAQNADVVIIDTAGRLHNKVNLMNE
                     LSKIKRVMQKVVPDAPHEILLVLDASTGQNAIEQCRQFSEATDVNALALTKLDGTAKG
                     GVVIGISDQFKIPVKYIGLGEGMKDLQLFNKTEFVDSLFKE"
     gene            complement(11666..11821)
                     /locus_tag="PK281_02025"
     CDS             complement(11666..11821)
                     /locus_tag="PK281_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010250750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4295 domain-containing protein"
                     /protein_id="HOY48146.1"
                     /translation="MAKKVVATLKTGAGKSFTKVIKMVKSPKTGAYAFKEEVVHNDHV
                     KDFLSGK"
     gene            complement(11832..12014)
                     /gene="rpmG"
                     /locus_tag="PK281_02030"
     CDS             complement(11832..12014)
                     /gene="rpmG"
                     /locus_tag="PK281_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HOY48147.1"
                     /translation="MAKKGNRVQVILECTEHKTSGVPGTSRYITTKNKKNTTERIELK
                     KYNSVLKRVTVHKEIK"
     gene            complement(12175..12972)
                     /locus_tag="PK281_02035"
     CDS             complement(12175..12972)
                     /locus_tag="PK281_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014031484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HOY48148.1"
                     /translation="MNAHEIDYRIIGSEMQCVEIELDPQEAVVAEAGSFMMMDQGIKM
                     ETIFGDGRAQEQSFFGKLMTAGKRVLTGESLFMTVYSNVGQGKQQVTFAAPYPGKIIP
                     LDLTMYQGKIICQKDAFLCAARGVSIGIEFQKRLGTGLFGGEGFIMQKLEGDGWAFVH
                     SCGTIIERELNPGEILRIDTGCIVAFTQNVDYDIQFVGGIKNTLFGGEGVFFAQLTGP
                     GKVWIQSLPFSRLAGRVLRYAPGMGRGGKEEGSLLGGLGGLLDGDNR"
     gene            complement(13021..14145)
                     /gene="dnaN"
                     /locus_tag="PK281_02040"
     CDS             complement(13021..14145)
                     /gene="dnaN"
                     /locus_tag="PK281_02040"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008616076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOY48149.1"
                     /translation="MKFIISSSTLLKQLQAISGVLSTNSSLPILEDFLFEISEGELKV
                     FASDLDNTISTTFKADAAEPGRVAIPAKMLLETLKSFPEQPLTFLINSENFGIEIASG
                     YGKYKLNGHDPEEFPRIPEIEDGAGFQVDASILFSGINKTIFATGNDDLRPVMSGVFM
                     QVGPDHMTMVATDSHRLVRYRRLDVKAAADASFILPKKPLNLLKNLLGYVDGEVAVSF
                     NAKNASFSFDHINLVCRLIDGRYPNYEAVIPKENPYKLTMARQDLMTSIRRVAIFSSR
                     STNQVKLNIQGSELQLTAEDPDYSNSASERLTCQYEGEDMEIAFNSKFITEMLSNLET
                     ERVNLNMSAPNRAGILLPDADTSDKDEDLLMLVMPVMLNN"
     gene            complement(14242..15255)
                     /locus_tag="PK281_02045"
     CDS             complement(14242..15255)
                     /locus_tag="PK281_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48150.1"
                     /translation="MKSNRILILGIVLLVAVLGYLYMHNGRTTLGGDLKNFTVEDTAT
                     VDRIFIADKQNHKVLLERKGPALWTVDGKYNARQDGIKMLLETLNKMVVRNPVAKSME
                     QKVLKDLAGPTQRKVEIYQKGELTKVFFMAAETPDKMATYMLMDGAETPYEIHIPGFK
                     GFLQTRFNSDGRLWRDPVIFRYDYRDMRSVEVKYPGMPQDGFTVKYDGKITRLFHPGN
                     PIPDPALDSLRAFEYLNGFRNIGYEFEVVETFSKAIKDSILASSPFIEVSVTDKNGSV
                     NSMKGYRRPSNLVSEVAEGEVKPPFDVDRMYGMINNGKDFVLIQYYQFDKVIKPAGWF
                     LRR"
     gene            complement(15261..17015)
                     /gene="gldG"
                     /locus_tag="PK281_02050"
     CDS             complement(15261..17015)
                     /gene="gldG"
                     /locus_tag="PK281_02050"
                     /inference="COORDINATES: protein motif:HMM:TIGR03521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     substrate-binding protein GldG"
                     /protein_id="HOY48151.1"
                     /translation="MAKVPSPSSRLKRSAILNLVLLVVLLILANLAASFKFTRFDLTS
                     ERRYSLSDATISLLENLDDYVYVKVYLEGDLPANYTKLRNSTKELLDEFRAYSSFVEY
                     EFINPSESENEKERRAIYQQLLDAGLTYTNPVEQKASGVSQTLIWPGALITFKTRTLP
                     LQLLRSQTYANEEELITRSVNDLEYEMTNTIRKLGTVIKPRICFIEGHGELDSLDTKD
                     VTISLEEYYAVERKRLDSNLTSLVLRAEKGDTVKFIPRYKAIIIARPDSAFSEKDKFI
                     IDQYIMRGGKVLWLINKVDASMDSLNVYSNKFVYPIDLNLDDQLFRYGARVNPDLVAD
                     LRASVIPVVAGMVGNQPRFIPKRWPFFPLSLPASKHPVVNNLNATRFEFVSTVDTVGA
                     PDIKKTILLSSSKRSRILSTPARISLNILRETPDPRQYSSSGLPFAVLLEGSFTSIFR
                     NRMSPQIRNSDKIGFLESSVKPGAMIVVGDGDVIKNSYSRTTGRMSPLGYDRYTGELF
                     GNKDFILNCVNYLCDDSGLISVRSREVKLRLLDETQVKSSRTSIQIRNVLVPILLILV
                     SGLILSFLRKRKFGRNHS"
     gene            complement(17018..17749)
                     /gene="gldF"
                     /locus_tag="PK281_02055"
     CDS             complement(17018..17749)
                     /gene="gldF"
                     /locus_tag="PK281_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     permease subunit GldF"
                     /protein_id="HOY48152.1"
                     /translation="MIALLRKEISSFLTSLIGYSVITIFLLAIGLFLWVFPGNFNILD
                     AGFASLENLFVIAPWVFMFLIPAITMRSFAEEQRTGTIELLLTRPLSEWQIITAKYLA
                     GLLLVIFAVLPTIVYYFSLKSLGNPVGNIDSGGTWGSYLGLIFLGGSFVSIGLFASSL
                     TENQIVSFLIGFFLCFFFYAGFDSIASMELFQPLDQIIIKLGINEHFGSMSRGVIDSR
                     DLIYFISFTLFFLALTRFRLLSRTW"
     gene            17973..19301
                     /locus_tag="PK281_02060"
     CDS             17973..19301
                     /locus_tag="PK281_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOY48153.1"
                     /translation="MIIEQLYDKGLAHGSYIIISDGQAAVIDPARDPQPYYDFVEKHQ
                     AKIVAIIETHPHADFVSSHHEIAQKTGATIYVSKLLGADYSHQTFDDGNEISLGKIKL
                     VAMNTPGHSPDSICVLLKDEQGKDHSLFSGDTLFVGDVGRPDLRESVGNLTAQREELA
                     RAMFHSVQDKLKKLHEEVLVYPAHGAGSLCGKNLSSDLSSSIGRELKTNYAFQISDES
                     EFVKALIQDQPFVPKYFGYDVNLNKQGAASFEASIAAVPRIASNSVLEKGVLIIDARP
                     QTDFRNGHIYGSINLMDGGKFETWLGSVVGPEEQFYLIAESVEKLEILIRKAAKIGYE
                     SRIKGALTPPAQMIETLEEVDVDDVRDLKELYTIVDIRNTGETATRRIFPGALEIPLP
                     ELRERIEEIPTNKPILVHCAGGYRSAAGASIIQPFVNVPVYDLGEAVLTF"
     gene            19311..19733
                     /locus_tag="PK281_02065"
     CDS             19311..19733
                     /locus_tag="PK281_02065"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOY48154.1"
                     /translation="MKNILLLLSILVAIPTIAQKKGRLEKLDPKAFQVKTVHLKGTLL
                     DVRSKDEFTAEHIKSAKNLDWDSKEFKDIAKKLPRYEPVFVYCQGGYRSNLAAEWMLE
                     EGFSTVIILDNGIDAWKDSGLPVEGENAPNKKSDSSYD"
     gene            complement(19805..21445)
                     /locus_tag="PK281_02070"
     CDS             complement(19805..21445)
                     /locus_tag="PK281_02070"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY48155.1"
                     /translation="MKNLFTFLLIVLMGFALTAQTPFFSDVTYRGAFAPAPAAMWTDG
                     WCNWDPQNTNYGTPNVNVSANISSNTTWTAGNVYLLQGQIYVTNNATLTIEAGTVIQG
                     DINTSGSGLFVTKGAKLNANGTASQPVVFTSSQPAGSRNLGDWGGVILLGKGNSNSVG
                     GVANIEGIAPTSDTEFGGGTSPDDNDNSGTLTYVRIEFGGYVYAPNKEINGLTLGAVG
                     RGTTINHVQVSYANDDAFEWFGGSVNCSHLVAYRNLDDDFDTDFGFNGNVQFCLAVRD
                     PEISDNPTVSTSEGFESDNDAAGDALTPLTAAIFSNVTIVGPLRGNVSSTVAEGYRRA
                     VRIRRNSNLKIFNSLFMDHSKGVMIDGTACEANATSGALSFKHNILAGNTAGNVCEVT
                     SGSTFDIHAWFAASMNDSLVATTGILATPYDYLAPDYRPAGSSPALSGADFEDAVFSG
                     LVLSANTQIGNLDQAVIFPNPANDFVTINFTAKNAGSVSVVISDLSGRMIMNNASSKL
                     VAGKNSLTINTEALSSGMYFVTLSDGKSQQSFKVTISK"
     gene            complement(21673..>23123)
                     /locus_tag="PK281_02075"
     CDS             complement(21673..>23123)
                     /locus_tag="PK281_02075"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOY48156.1"
                     /translation="PNLRRNVYTRLDHSNDPGEPTNRDTVWRANISKTNVGPDYGGGM
                     FFSENKEQVYSFRADAFYPVKIGKELNNDVKIGGSFQQRDREFFARQLGYTQYSTIGG
                     GSVKFQDSLTYLSDDQIFASTSMGLIAPGKGGFKLKDATKNSDSYSAGSKLSASYVMI
                     NTRYKKWLRLVWGARLEAFTQTLDATLDNNKPLSIRTNKVDILPSANAVISVSEKQNI
                     RASYSQTLNRPEFRELAPFAFYDFNTQFVYSGNDTLKRALIHNYDVRWELYPGRGQLI
                     SATVFYKSFINPIEQISRADVANEVSFKNVAKANNYGAEFEFRTIIGSVLKKSDTHFL
                     NNLTVYANYAYIRSAVDVSDVLGAKDDSRPLQGQSPYVFNAGLIYANKDKGYSVSAAL
                     NRVGTRIAIVGNVNEPDIWENGRTVLDIQASKSFLKQQLEVKFFVRDGLAQKLYFFQD
                     RNDNQKLDLDSDNMIWVSKFAPTFSMNVTYKF"
BASE COUNT         7171 a         4618 c         4585 g         6749 t
ORIGIN      
        1 cgattgctca ttataagcgg tccgaatgcc ggtggaaagt cgattacgtt aaaaaccatc
       61 ggattgcttc agatgatgct gcaaagtggt atgcttgtcc ctgttgggga acaaagtgag
      121 atgggatatt tccagcgttt gtttgcggac attggagata gtcagtcgat tgaatacgag
      181 ctcagtacct acagttcgcg tttagcaaaa atgaagtata tactcgaatt tgctgataag
      241 aaaacgctgg ttctgattga tgaatttggg accggtactg atccggaatt aggtggagct
      301 gtggctgaag cgatgctaga ggaattgtcg cgtcggcgta ccttgggagt tattacgaca
      361 cattacatga atattaaatt ggcggccgat cgcttggatg gagttcggaa tgcgtgtatg
      421 ttgttcgatg atgagacttt acaaccgatg tatcagttgg ttattggtca acccggaagt
      481 tcgtatacct acattattgc cgaaaaaacc ggattgcctg tagccttgat taatcgggca
      541 cggaacatga cttctaagga taaggtgact ttagatcgta tgttgcagca attgcaaaaa
      601 gagcagcagg aattgcgtaa actacttcat gtattagatg agaagcaggc tattgcagat
      661 aaatcgaaat cgaaatacga tgagcttcac gaaaagatga agctaaagat ggagagtaaa
      721 aaggcgaccc aagaagaatc ggggaagttg atcgaattag gcaaaaagct acaatctatt
      781 accaaagaat gggaagctgc taaggacaag aagcccattt ttgccaaact gactaaaatt
      841 ctatcggctg aaaagcgcaa gaagattgac aagaaacagc aattgaaaaa agagaaagtc
      901 aaggctcaat tgctcgagaa gaagaaagca gaaatcaagg ttggatcgaa agtgaaatta
      961 ctcagcggtc gtcaaacggg catcgttgaa gaaatccaaa acaaccgtgc gcgtgtgatt
     1021 ttcgggaatt tgaaaaccct agctagttta gaaactttag aagttgttga agattagttc
     1081 cccaataaag taacggtccc tttttcttcg aacggttgat ctttaatatc ggttcccgaa
     1141 ataatgaaga aataagtgcc atccggaact ttctctgaat tgtgcaatcc atcccagcca
     1201 gtactcacat tttcccatga atacagttgt tttccccaac ggttaaacac gcgagcttga
     1261 aaggttttta ctgcgttgta cttcacctga aagaggtcat taaaaccatc tgaattagga
     1321 gtaaatacat tgggaacaac taacaataat ttatcactca acactttgat cacagccgaa
     1381 tcggaatcat aacatccagt agaattacta actactagtt ccaaaacaaa agtaccagaa
     1441 tcaggaaacg tgagtgtgcc tggagctgta aatatgagag gctcaccatt caacgtccag
     1501 ctataagttt ctccattaac actttgatct gtcacattca caggtaaaat cacataacca
     1561 gaatccacat cggtagtgaa attcgctgtt actggaatac ccgtaataat gatctgatca
     1621 gtagctgtat tgcattgccc agatcgtgtt aaggtatacg ttccagctga agtaaccgta
     1681 attgaacttg ctgttgatcc tgtattccaa gagttttgat tgtttgctgc agaggttaat
     1741 tcaattggtt gtccactaca aagtgaagtt tcacttggag gaatgatttc ggccgttaat
     1801 agtccaattt gttggatgat aatctctaat gcttcacttt cgcatgcacc tgaagttgca
     1861 gtcacataaa gtgtatcatt ctcaatagat gactgataat tcaaacctgt tgcgataact
     1921 gctgataagc tttcatcgga ataccaagtt ataggagaag tactgctttg tacagaagca
     1981 agagggattg gatctgcttc acaaaaagtt aacaatgtat caccagttgg aattgcaaca
     2041 acagaaaatt caacagttac accagttgta tctgaataac aaccttgttc agattgaaca
     2101 gcataatatc cggcattttg tccgtttgct ggggtgatgg aatttccatt agataagaat
     2161 gtagtaagag ctggatcgga gtaccaagaa acagtggcgt taggatcagc gtttatagaa
     2221 agagcattgg gagtttcacc attgcaataa agagtatcac cagtaatgtt tattggggat
     2281 agactaacta cttgaagggt ttgagatagt atatctgaac aagttccagt acctacagaa
     2341 taggtaattt gaatattacc atcgagccca tttaagttca actgagagcc agtaacaccg
     2401 tttcctgacc aagttccgcc agttgttcca gtaaccaatg tatctaaatc aatagaacca
     2461 agtgtagcgc aaagcaattg gggatttgta aaggatgcat ccacactttc gctaacgata
     2521 atatttaagg ttaaagatgc cggttgagta atgcacccac ttggaggagc cacactatat
     2581 gtaacagagt atgaaccatt tagtccaaca ggattgaata tagaacctgt tacaccagtt
     2641 ccagaccaag taccacttga tgaacctgta acaaatgtat ttagatctac tgctgtgcta
     2701 tttccacaaa aaggtcccgg attaacccaa tcggccgaaa agggttcaat tggagctgta
     2761 catccagtat tggtatagct agctgtacca tcatctgaaa actcgaccgt ggcgccattt
     2821 tgagcaacat tttgtccgtt cgtattaact agtaaagcac gttggtaggt aacttcttca
     2881 tagcaagtat tagcacccgt aatttgaatt ctcagtgttt gatcttgtgg agcgcccgtg
     2941 ttttgattta gaaagtgtcc tccaccttcg cctgtatgtt gatgataaat gatgtaaagt
     3001 gtatcactta agtttgcgaa agaatttaag gatgcagaaa catcgaaact ggtaattgcg
     3061 agaactttac ggttttgggg aataattcca ttaggaggtt caagcaaata accacaagaa
     3121 atgatggtgt tgtttaattc gacagtttta gcttgtgtgg atgaattctg aacgaaacca
     3181 ttaaaaggta atgcattaac actttcggaa ggccaaccat ctaagattgt aatgtctgag
     3241 atattcaaat caaaattccc attacgaaac cgaaacattt catttagtcc ttccggatta
     3301 gtgaatccat catcacaggc atcaaccaat acactttcaa tttgaataca ggattgagcc
     3361 gaagatgtga aaggtaaaag gaatgctatt aaagctatta aaatgagtgg gtacttcata
     3421 ataatcagga attcttaacc tagtttgaat ctcgaatata cataatagat ttcttactaa
     3481 aaaatgaact gtcaaaagaa gaattcatcc tgaaagaaaa aatgaaaaaa aatccgataa
     3541 acctaaatgg cttatcggat tttaactgtt taagaaaata cggaaaatac ttaactgaaa
     3601 ttcagttcaa tattaatttc tcacaagtgt aattgaacca tagcgttcaa aatcattgct
     3661 ttggaaatct ttacctttta tcacgtagaa ataaacccca tcagaaactg gatttccctt
     3721 tttagttcca tcccagctat ttccagcacc ttcccaagta taaatttctt gtccccaacg
     3781 atcaaaaata gtagagcgga attctacgat tccagaagat ttcgctttga aaatatcgtt
     3841 gtagccatca ccattcggag tgaaactatt tggtaattcc aatacaaaac taccgttact
     3901 tactacaatc acacgagaag tactatccat acatccgtta ttatcgcaaa cttgtttcaa
     3961 tgtatatgtt ccagcatttt caagcaggag agcattttga gagataatgg tagaagaacc
     4021 attcaaatac caatcgcaac ctgttgaaga agcaggtact actgctaggt taagtggtgc
     4081 atatccttca agtgtactca catcaaagga agcatctgga ctaagatcaa gcactacaat
     4141 ttgatccgtt gctgaattac aagaatttga agcagaaact gaataagtcc cagcttgagt
     4201 gacaataagg tcgtttgttt gagctccagt tgaccataca aaacttatgt tagaagtagc
     4261 ttgaagagca actgaactac catgacaaat tgttgatgga ccagtaacag agatagccac
     4321 cgttgctaat ggtaagccat taacttgaac aacaactggc tcactaacac aaccattttc
     4381 tcgtgcaatt acccaaagcg taattccagt ttgagttggt tggtaggtag gtccttgctg
     4441 aactaaattt tgcatatcta catccgaaaa ccaataaaca ttagaggtag atgttgctgt
     4501 taaaactgga atagctgtac cctcacacac attaacatta gcatctgtta taggtgaatt
     4561 aggagtaggg actatcgtgt agctaaaact actaacttca gaagtacaat cacctaaaga
     4621 ttgaactaca taaactgttt gatctcctgt tcctgaagga gtgtatgttg ctcctgttcc
     4681 tatttgaata gttagttgac tatcagaaaa ccaagaaata gttgcacctg gaagagcggt
     4741 aacatcaaaa ggtgttagtt ctcctccact acaaaacaag tctgaaccat ttacttcaag
     4801 aacaggtata gtacctatgt atatggtttg tattactgta gatacacagc cattgcctga
     4861 aactccatat cgaataacag tagaatcaac aactaaagaa ggatcaaaag tgcttcctgt
     4921 aactccatct cctgaccaag taccaccaag atcaccagtt attaaaccat ccaaattgat
     4981 taatgtagat aaatcacata taaagcccgg gttagtccaa cttgcatctg gagctacggc
     5041 gacagtaact ggctgagtat tggatcctga acagccatta atgtcttgag agaaagtgat
     5101 aattacctgt ccagttaaac ctgttggatc gaatatgcta tctgtaatat tttgtccagt
     5161 ccaagtacca actactccgt taggaagttg atcatttaga ttgattgttc catttcctga
     5221 acatatcgta cctggattaa cccagttaac attgattggt tgaatctgaa tagaatgaga
     5281 ttctgtttga gtacaaccac taacacctac tgtataagtt agtgtaattg atccagttaa
     5341 acttgttgga ataagattat tgccatttac tccttgtcca ctccatgtcc caccggctgt
     5401 tcctgacaca tattgagtta aatcaactgt tccagcatta ctacaaatta atggaggagc
     5461 agtccaattt ggactaacag caccatgaat tgcaactgtt tgagtatttg attgagaaca
     5521 agatcctgca ccaacggtat acgtgatatc tactgacccc gataaacctg ttggatcgaa
     5581 tatattgcca gtaacacctg gtccagacca tgtcccacca gtagttccgg ttattaaaag
     5641 atttaggtta aatggctgtg aattagcaca tacattggga gcagtccagt ttgcagagaa
     5701 aggagtaata ggtgcaacac agccaatgtt gatgtaagtg gcatcacctt gaatgttgaa
     5761 gtttactgtt gctccatcct catcaccaat attaccagca gttgtaatta attgcgttcg
     5821 ataataggtt acttgttctg tacagccacc aattgttgga aaatctacat acagtgtttg
     5881 attattggga ttagcattag ggacataatt gagaacgtga cctgtattgg tattctcatt
     5941 agcataaacg atgtaaaggg tatctcccaa ctgtgaaaag aaattcgtag aagtattaca
     6001 gttatagcta gaaataagta aaacatcatg atgggcaggt ataattccat ctaaaggttc
     6061 aatcaaatat ccgcaggata gaattgtggc attaaactca gctgttgttt gagccgtacc
     6121 tgcatccatt acaataccgt tccaagacaa attagtattt ggccaagtta gattcatatc
     6181 gtaaatattc aaatccagca ctccggttct cattcgaacc atttcgtttt tcccttcccc
     6241 atttccattg ggggcacaag aattcacgag aatactcatg acatcaatac agttagactg
     6301 agcctgaata tgagtattgg ctaaacaaag acttagaaat aaaaatgaaa gtaaaaactt
     6361 cttcatgggt agaatttgaa aatattcttg cttgaaatca actgccaata taggagttga
     6421 aaaggaattc tcatattaaa aaataggatt caatcttcct gataaataag aagaaaggat
     6481 ttttctagcg gttgacaccc gtccataatt acatcaagaa tgagaaatga atgtctcaag
     6541 atgaaagtac tgattgattc tttacgctcc tgaagtgttc cacctggata aatatgatta
     6601 aacacttcgt tcaaccgatt gatttctatg gcacttgctc ttttttgcgc tcgaattagt
     6661 ttttgtgaat atcgacccaa ttgatcctcc aaacgttttc ctaaagcttc agcacttgag
     6721 actaaggtag tatcaaaacc ctgagcatga tttttcaact gattaattag tctagttaaa
     6781 tccgaaaatt cgttctttaa ttccagatca gattcactga gtgataaagc gatttgcttt
     6841 tctaactctg atcttgtttt aaacaaatct tcttcttgga aacgagcatg ttcaatacgc
     6901 aaagtatttc tacggtcgat tattagcgcc gaattcctga gcattaaagc aggaaaaggc
     6961 atccgataat gatcgaaaat ccccttcaac tctaaccaat acgaaatctc agctcctccg
     7021 ccgaaatagg caatattggg caatatcgtc tcctgataaa gtggtcgcat caacacattg
     7081 ggtgaaaatt tatctggatg ctctgaaatc aatcccttca attcctccga attccaactg
     7141 tgtttcccat catgtgtcaa gaaccttccc tcctcttcta ctatccgctc tcgatatcca
     7201 tcttccaaaa agaaaaagtt tatttctctt ccattcactt gagaatgata acgttcctct
     7261 agtttttctt tggtctgact tacctctcta aaactatttt tctcaagtat atcttgaatt
     7321 ataatgggag caaatgcctt tttcaatcgc gtatcatccg catcaataac taaaagcccc
     7381 caatgcccaa atagctggtg gacaatgtat cgtgttgcta aagatagatt tggtaaagca
     7441 taagcgtttt tgaatatctc tttaagtcga actgcatccg gacttttacc tgcaatttga
     7501 tcaaactgca ctaaaacttc ctccatccca tctaacgaaa accgaccaac tggtccagtt
     7561 tgatttgaat tccactgtag tttttgtcct tccacccaaa cgtggtcaat ttcggccaca
     7621 tcatgatctt cagttgccat ccaaaaaatt ggaacgaact gattttgagg atacttattg
     7681 cttaattcct gcgacatttt gatagcagaa attattttca aaggagaata taatggtccg
     7741 agcagaattc cagtttgttg tccggtagtg atggtaaatg tatgctcgga tagcagcttg
     7801 gaaatctgtt cctcgactat tgaatcaaga atgccggctt gcttgtattg gtcgcgcaat
     7861 acttctgtaa gaacaacccg gtcgactgtt ttttgacttt tctgatggat aaaatgttgg
     7921 aaattatcca aggttggagc agcttcataa aagggcttca agaaaggatc ttgatccaaa
     7981 taatcgcaca cgataggttg aaacttcccg gttttccggt aaggaacgta gctacatcgt
     8041 gtcttcattt cacgaaagta ccgaaaagat caaattcttc agcccgatca atttttacct
     8101 gagcaaaatc tcccacgcga acgtatgaat catcttttgc ttctactaaa acttcattat
     8161 ccacttcggg tgaatcgaat tcagtacgac caataaaata aggtccttct tttcggtcga
     8221 ataatacttt gtaggtattt ccaacttttt cttgatttag cgaagctgaa atgcccatct
     8281 gaacatccat taccgcatca gcacgttctt gttttacatc cgcaggaaca tcatccacta
     8341 aagaataagc atgtgtgttt tcctcatgag aataagtgaa tacacccaaa cgttcgaatc
     8401 gactatcctg gatccattgc aacatttcct cgtgatcttt ttctgtttca cccggatagc
     8461 ctgcaattaa tgaagtacga atcgccatcc ccggaacttt gtcacgaatc atattcacta
     8521 ggtcaattgt cttctgcttg tttgtaccac gacgcatgct tttcaacatc gaatcactga
     8581 tgtgttgcaa aggcatatcg atgtacttgc agatattttc gcgctcacgc attacatcca
     8641 aaacatctgt tggaaatcca ctagggaaag cataatgcaa tcgaatccac tctaaaccat
     8701 tgatatccga aagtcggctc atgagttcgg ccagcttccg gtctttgtat aaatcgagtc
     8761 cgtaataggt tgaatcttga gcgattaaca gtaattcttt agtcccattt ttaaccaatt
     8821 gcttcgcttg cgcctctagc tgttccatgg gaatggaaac gtgtcctcct cgcataagtg
     8881 gaatagcaca aaaagaacaa ggacgatcac aaccttctga tatcttgaaa taagcataat
     8941 gagccggagt agtgagtaaa cgttcaccca ctaattcgtg cttgtaatcg gctttgagtg
     9001 tttttaataa acggggcaga tcacgcgttc caaaccaagc atccacttcg ggaatttcgg
     9061 cttctagttc gggcttgtat cgttcgctca aacaaccagt tacgtatact ttatctactt
     9121 gtccgttggc ttttgcctcc gcatacctta ggatggtatt gatactttct tccttagcat
     9181 tatcaatgaa tccacaggtg ttgatgatta caatttcgga atcatcttgt ttggattcat
     9241 gttccacctc gaacttattc gccttgagtt gccccatgag cacttccgaa tcgtaaatgt
     9301 tcttggaaca gccaagtgta accacattga ctttattttt cttgagggtt ttggtcttca
     9361 tcctatgcag aggaatttat tcggattggg gcgcaaaggt acgcatttca tagggtataa
     9421 aaagaaaacc agtcaacccg aatgaacgaa ttggctggtt taaattttaa taaccggtta
     9481 gttccgaatg aatttcttga ctgttattcc atttggactt tggagctgca ggtaataaac
     9541 acccggttgg atagccgaca agtcgaattc ttgaatggta gtagcattcg atttagttgt
     9601 ttgcatcgtg cttacaacac gtccagataa atcggaaaca agtattttct ccacattggg
     9661 ttgaatactc acgttgattt tcccagaagt tggatttggg aataattgaa taatatcgga
     9721 agattgtttc tgaatgcctg tattatcgtt gttataagag tgactggcat ttacactata
     9781 cgttggagtt ccatcactaa tagttgtatc gtaagtgata gagaaaattg ggattgttcc
     9841 accttcaata acgcttaacc attcatacat agtagagcga gattctgtgt aagaagtatc
     9901 cgtttgtccg aagaaagatg aataggcagt atcaacactc agtgtacgat gtttaaaacg
     9961 tagcacattg gtatatgtac ctgaccaatt gaaaagtgtt ccgtaggcat caacaacata
    10021 agaagatgtc ccggttgtac ttgtaaatgt tgaacctatt cctatatcta gcgcgacttc
    10081 atagcgaaaa ggatcactta cagtgctatt gaaagttgct gggaattgat attgaatttg
    10141 aggatcagta taaatcatat taatatcccc aaaagtagaa ctcacgaaag tgccttgctg
    10201 ttgaacattg ttcgagttag cacttaaaaa tgtgtagaaa ttgtttccgc cagtttgagc
    10261 acctaaagca atattagcgg ctggaaatga actactttga gaaatagctg aagcatcgaa
    10321 aaatgttaag gaagtaacat caccggtgtc ggctaacata gaaaagtccc acattacatt
    10381 agcgccagca gcaccttcgt tagcagatcc tagttcgaca gaagaggtag acaaagaata
    10441 acctacagat ggcatgctgg ggaatgtcat tgtaggttgc gcaactagtt gcaaagaagc
    10501 aaatagcgtt gataagaaaa gaatagggtg tttcatcgta gtaaaaaatt attgaacgtg
    10561 aatgagaccg caaagactaa gcctttgtta cacgtttgat aaaattttac taagcttttt
    10621 actctttgaa aagagaatca acgaattcgg ttttgttaaa gagttgaaga tccttcattc
    10681 cttctcctag accaatatat tttacgggga ttttaaattg atcagaaata ccaatcacga
    10741 ctccaccttt agcagtgcca tctaatttgg taagcgctag ggcattcaca tccgttgctt
    10801 cagaaaactg acgacattgt tcaattgcat tttgaccagt tgatgcatct aagaccaata
    10861 aaatctcgtg tggagcatcc ggaacaacct tttgcatgac acgtttgatt ttactcagtt
    10921 cgttcatcag gttgacttta ttatgaagtc gcccagctgt atcgataatg accacatcgg
    10981 cattttgagc ttttcccatt tgcactgcgt cataggcaac agcagcagga tcggcattca
    11041 taccatgttg aacaagtggc actcctaccc gatcggccca gattgtaatt tgatctacag
    11101 ctgcggcacg aaaggtatct gcagcaccga gcacaacaga ttttcctgct tctttgaatt
    11161 gataagctag tttggcaatg gtggttgttt ttcctactcc atttactcca acaaccatga
    11221 ttacgtatgg accatcggtt ttgggaaggt caaaatcaac taggtcgcta ctgcgatttt
    11281 cttcaagtag agcggctatt tcttctttca gaattccgtt gagttcggaa gaagtagtgt
    11341 atttatcgcg accaacacga gcttcaatac gctcaataat tttgagtgtt gtactaacgc
    11401 ccacatcggc tccaatgaga atttcctcta gattatcgag gacttcttca tctaccttcg
    11461 atttaccaat gagaaccttt gccagtttgg aaaagaaact ctctttggtt ttggcaagcc
    11521 cttgatcgag ggtttccttt ttctcacgtg aaaacaaacc gaaaaatgcc atagatgtgg
    11581 gattgaaata ggctgtagcc tgttagcaaa aaaagctcct tccggtatgg aagaagctta
    11641 caatcttgga tgcatccatt aaaaattatt tcccgctaag gaaatctttc acgtggtcgt
    11701 tatgaaccac ttcttctttg aatgcgtatg cgccagtttt tggcgatttc accattttaa
    11761 tcactttggt aaatgatttt ccggcgccag ttttcagggt tgcaactact ttcttagcca
    11821 tggttcagcg attacttgat ttctttatga acggttacac gtttcaacac tgaattgtat
    11881 ttcttcaatt caatacgttc cgtagtgttt tttttgttct tcgttgtgat ataacgtgaa
    11941 gttcccggta cgccggaagt cttgtgctcg gtgcactcca ggataacctg aacacgattg
    12001 ccttttttcg ccatggcgat gaatctttag ttttaagggc tgcaaatata gtcaaatcat
    12061 ttttccgtca aacagtatct gagaaaaata attaaaaatg tgctaatgtg ctggtgtgct
    12121 gatgtgctag ttgcttagtt aattggcaca tcagcacatt atcaaatcag cacatcatct
    12181 attatctcca tccaacaagc cacctaaacc gccaagtaaa gagccttctt ctttaccgcc
    12241 acggcccatt cctggggcat aacgtagaac acggccggca aggcggctga atggtaagga
    12301 ttgaatccac acttttccgg gaccggtaag ttgagcaaag aaaacgcctt caccaccaaa
    12361 caaggtattc ttgattcctc ctacaaattg aatatcgtaa tccacattct gggtgaatgc
    12421 tacgatacaa ccggtgtcga tgcgaagtat ttcgcctgga tttagttcgc gttcgatgat
    12481 ggtaccgcat gaatgaacaa atgcccaacc atcaccttct agcttttgca tgatgaaacc
    12541 ttcaccacca aataaacctg tcccaagtcg cttctgaaat tcgatgccaa ttgaaacacc
    12601 acgagcagca cataagaatg catctttctg gcaaatgatt ttcccttgat acatggttaa
    12661 atcaagtggt atgattttgc ccggatacgg cgcggcaaag gttacttgct gctttccttg
    12721 tccgacgtta gaataaaccg tcataaacaa gctttcacct gttaaaacac gtttaccggc
    12781 tgtcattagt ttgccaaaga aactttgttc ttgagcgcga ccatcaccaa agattgtttc
    12841 catcttgatg ccttgatcca tcatcataaa tgatcctgct tcggcaacaa ctgcttcttg
    12901 tggatctaat tcaatttcta cacactgcat ctcggagccg atgatgcggt aatcgatttc
    12961 gtgagcgttc ataggttata ttttatgcat gaaaaaggcc ggaaaaccgg ccctgtgaaa
    13021 ttagttattt agcattactg gcatgaccag catgagtaaa tcttcgtctt tatcgcttgt
    13081 gtcagcatca ggtaataaga taccggcacg attaggtgcg ctcatgttca aattcacacg
    13141 ttccgtttcg aggttactca acatttccgt gatgaatttc gagttgaatg cgatttccat
    13201 gtcttcacct tcgtattgac aagttaaacg ctcagatgct gaatttgagt aatctggatc
    13261 ctcagcagtt aattgtaact cgctgccttg aatgttcagt tttacttggt tcgtactacg
    13321 actagagaaa atcgccacac gacgaataga cgtcatcaaa tcctgacgtg ccatagtgag
    13381 tttgtatgga ttttctttcg ggataacagc ttcgtaattc gggtaacgac catcgattaa
    13441 acgacaaacg aggttgatat ggtcgaacga gaaggaagca tttttagcgt tgaagctaac
    13501 agctacttcg ccatccacat aaccaagtag gtttttcaac aggttaagtg gcttcttagg
    13561 aagaatgaaa gaagcatcag ctgccgcttt cacatctaaa cgacggtaac gaaccaaacg
    13621 atgagaatcg gtagcaacca ttgtcatatg atctggacca acttgcatga atacgcccga
    13681 cattacagga cgtaaatcat cgttaccagt cgcgaaaatc gttttgttga ttccgctaaa
    13741 aaggatggaa gcatcaactt ggaaaccagc accatcttca atttccggaa tgcgtggaaa
    13801 ttcttctgga tcgtgaccat taagtttgta tttaccgtat ccagaggcga tttcgatacc
    13861 gaaattttcg ctgttaatca ggaatgtaag tggttgctca ggaaagcttt ttaatgtttc
    13921 gagcaacatt ttggctggaa tggccacacg tcctggttca gctgcatctg ctttaaaagt
    13981 agtgctgatg gtattgtcaa gatcagatgc aaacactttc aattcccctt cggagatttc
    14041 gaacaggaaa tcttccaaaa tgggcagact gctattggtg ctcaatacac ctgaaatagc
    14101 ttgtagttgc ttgagaagcg tactgctgga gataataaac ttcattgctt aattttttaa
    14161 ggggcgtaaa gatagcggat tgagagatat tccaatcaaa tcaatttcgg ttctcaaggg
    14221 aaaagatttg aaccaagctg ttcatcgtct caaaaaccat ccggcaggct tgataacctt
    14281 gtcaaactga tagtattgta ttagaacaaa atctttcccg ttattgatca ttccgtacat
    14341 cctatccaca tcaaatggtg gctttacttc tccttcggca acttccgaaa ccaaatttga
    14401 gggacgacgg tagcctttca tcgaattaac tgaaccattt ttatcggtca cacttacttc
    14461 aataaatgga gaagatgcga gaatggaatc tttgatggct ttggagaatg tctctaccac
    14521 ctcgaattca tatccaatat ttctaaaacc attcaggtat tcaaatgcac gaagtgaatc
    14581 gagcgctgga tctggaatag gatttccagg gtggaataaa cgggtaattt taccatcata
    14641 cttcaccgta aaaccatcct gaggcatacc tggatatttt acttcaaccg agcgcatatc
    14701 gcggtaatcg tagcgaaata tcactggatc acgccataat cgaccatcgg aattaaatcg
    14761 ggtttgtaaa aaacctttga atccggggat gtgaatctca taaggcgttt cggcaccatc
    14821 catcaacatg tatgtagcca ttttatcagg cgtttcggct gccataaaaa acactttcgt
    14881 taactcccct ttttgataaa tctctacttt tcgttgcgtt ggtccggcca aatctttcaa
    14941 gactttttgc tccatcgatt tagccaccgg atttcgaaca accattttat tcaaggtctc
    15001 taaaagcatt ttaattccat cctgacgagc attgtatttc ccatcaactg tccacaaagc
    15061 tggacctttt cgctccaaaa gaactttatg attctgcttg tcggcaatga aaatacgatc
    15121 tactgtggca gtgtcttcga cggtaaaatt cttcaaatca ccacctaaag ttgtgcggcc
    15181 attatgcatg tacaagtagc ctaaaacagc tacgagcaat acaataccga gaatcaaaat
    15241 gcggttcgat ttcatgcttg ttatgagtga tttctaccaa atttacgttt acgcaagaag
    15301 ctgagaatga gtcccgaaac taaaatgaga agaattggaa ccaacacatt gcgaatttga
    15361 atggacgtgc gagatgactt aacttgagtt tcatccaaca aacgaagttt cacttcacgc
    15421 gaacgaaccg aaatcaaccc tgaatcatca cataaataat tcacacaatt cagaatgaaa
    15481 tctttgttac caaacaattc tcctgtgtag cggtcgtatc caagtggtga cattcgaccc
    15541 gttgtgcggg aatagctgtt tttaattaca tctccatcgc ccactactat catcgccccc
    15601 ggcttaacac tcgattctag aaagccgatt ttatctgagt ttcgaatctg tggcgacatc
    15661 cgattccgga aaatcgaagt gaatgaacct tctaacaaaa ccgcgaatgg taaaccggag
    15721 ctggaatatt gtcgtggatc gggagtttca cgcaagatat tgagcgaaat tcgagcaggt
    15781 gtacttaaga ttcttgaacg tttggagctc gataacaaga ttgttttctt gatatcggga
    15841 gcgccaacag tatcaaccgt acttacaaat tcgaaacgag tagcattgag attattaact
    15901 acaggatgtt ttgatgcagg taaactcaaa ggaaaaaacg gccaacgttt aggaataaag
    15961 cgaggctgat tgcctaccat tccagccaca acaggtataa cagaagctct caaatcggcc
    16021 actaaatcgg gatttacacg agcaccatat cgaaataact gatcatccaa attcaaatcg
    16081 ataggataga caaacttgtt gctatacaca ttcaaactgt ccatggaagc atcgacttta
    16141 ttaatcagcc atagcacttt accgcctcgc atgatgtatt gatcgatgat gaatttgtcc
    16201 ttttctgaga aagccgaatc gggacgggca atgatgatgg ctttataccg cggaataaac
    16261 ttaacagtat cacctttttc ggcacgtaaa actaaactgg ttaggttgct atcgagacgt
    16321 ttgcgctcca cggcataata ctcttccagt gaaatggtaa catctttagt atcgagcgaa
    16381 tcgagttctc cgtgaccttc gataaagcat atacgaggtt tgatgaccgt tcccaatttg
    16441 cggatggtat tggtcatttc gtattctaaa tcgttaaccg aacgagtgat gagttcttct
    16501 tcgttggcat aggtctgact acgcaacaac tgcaatggta atgtgcgtgt tttaaaggtg
    16561 atgagcgcac cgggccagat taaggtttga gaaacaccgg atgctttttg ttcaaccgga
    16621 ttggtgtagg ttaagccagc gtccaacaat tgttgataga tcgcacgacg ttccttttcg
    16681 ttttcacttt cggaaggatt gatgaattca tattcaacaa aagaagaata agcacggaac
    16741 tcatcgagta gttctttagt tgaattgcgc agcttggtgt aattcgcagg taaatcgcct
    16801 tcgagataga ccttcacata cacgtaatcg tcgagatttt cgagcaatga gatagtagca
    16861 tcactaagtg aatagcgacg ttcggatgtt aaatcgaaac gggtgaattt gaaagaggct
    16921 gcaagatttg ccaagattag caaaacaacc agtagcacca agtttaagat ggcgctcctt
    16981 ttcaatcggg acgatgggga gggaactttt gccatactta ccaggtacgg cttagaagcc
    17041 ggaaacgagt taatgctaga aaaaacagcg tgaatgaaat aaagtaaatg aggtcgcgac
    17101 tatcaattac accgcggctc atagaaccaa aatgttcgtt gattcctagt ttgatgatga
    17161 tttgatcgag tggttgaaaa agttccatcg atgcaatgga gtcgaaacct gcatagaaaa
    17221 agaaacacag gaaaaatcca atgaggaagg atacgatttg attttcggtg agcgaagagg
    17281 cgaaaagtcc gatggagaca aatgaaccac ctaggaaaat tagtccaaga taagaacccc
    17341 aagttccacc tgagtcgata tttccaacag gattaccgag tgatttcagg ctgaaataat
    17401 agacaatggt gggtagaacc gcgaaaataa ccagtaataa tccggctaag tatttagcgg
    17461 tgatgatttg ccattcgctg agtggacgag taagcagcaa ttcgatggtt ccggtacgtt
    17521 gttcttcggc aaacgaacgc atggtgatgg ctgggatgag aaacatgaac acccaaggag
    17581 cgatcacaaa taagttttcg agcgaggcaa aacctgcatc gagaatattg aagttccctg
    17641 gaaataccca gagaaaaaga ccgatggcca gcaagaatat agtaataacg ctgtaaccga
    17701 tcaacgatgt gaggaaacta ctgatctcct tccgcaatag tgctatcatt cgaaagcccg
    17761 tgttttagca gggacgcgca aaaatacacc tttttaattc tgagcaacat gaacataaaa
    17821 tgatggttgt cggaaacact cctttcgacc aatcagcaca tcaaccaacc agcacatcag
    17881 caaaccagca caccagcaca ttaaaaaata gcacatcagc acactagcac accagcacat
    17941 caattacctt tgtgtccgaa aaacacccaa ccatgattat cgaacaactt tatgataaag
    18001 ggctagcaca tggctcttat atcatcatta gtgatggaca agcagcagtg attgatccag
    18061 cgcgtgatcc gcaaccatac tacgattttg ttgaaaagca tcaggcaaaa attgttgcca
    18121 taattgaaac acatccacat gcagatttcg tttcgagtca tcatgaaata gctcaaaaaa
    18181 ctggagcaac aatctacgta agtaagttac ttggagcaga ttattctcat cagacatttg
    18241 atgatggtaa tgagatttct ttaggaaaaa tcaagctcgt agcgatgaat acacccggac
    18301 attcgcctga tagtatttgt gtgttattga aagatgagca aggaaaagat cattctttat
    18361 tttcgggtga taccttgttt gtaggagatg ttggtcgtcc agatttacgt gaatcggtag
    18421 gaaatttaac tgctcaacgt gaggaattag cgcgtgcgat gtttcattcg gtacaagata
    18481 aactcaagaa actgcacgaa gaagttttgg tatatccggc acatggagca ggttcgctgt
    18541 gtgggaagaa tttgagtagt gatttatcga gcagcattgg acgagaattg aaaaccaatt
    18601 atgcctttca gatttcagat gaatcggaat ttgtgaaagc tcttattcaa gatcaaccct
    18661 ttgttccgaa atattttggc tacgatgtga atttgaataa gcaaggtgca gcgtcatttg
    18721 aggcaagtat tgcagcggtt ccgcgaattg catctaattc tgttttagaa aaaggtgtat
    18781 taattattga tgcacgtcct cagacagatt ttagaaacgg tcacatctat gggagtatta
    18841 atttgatgga cggagggaaa tttgaaacct ggttaggttc tgttgttggt ccagaagagc
    18901 aattttacct gatagctgaa agtgtagaaa agttagaaat tctcatacgc aaagcagcga
    18961 aaataggata tgaatcacgc atcaaaggtg ctttaacacc acctgcacaa atgattgaaa
    19021 ccttagaaga agttgatgtg gatgatgttc gcgatttgaa agaattgtac acgatagttg
    19081 atattcgcaa tacaggagaa acagctacac gccgaatatt tcctggggct ttggaaatac
    19141 ctttacctga attgcgtgag cgaattgaag agattccaac taataaaccc attttagtac
    19201 actgcgctgg aggttatcgt tctgcggcag gtgccagtat tattcaacct ttcgtgaatg
    19261 tgccggttta cgatttaggt gaagccgttt tgacttttta atttattccg atgaaaaaca
    19321 tcctacttct tctttccatt ttagttgcaa ttcctacaat tgcgcagaaa aaaggtcggc
    19381 ttgaaaagct tgacccgaag gcctttcagg ttaaaactgt tcatttaaaa ggaacattat
    19441 tggacgttag aagcaaggat gaatttacgg ctgaacatat taagtcagcc aagaacttag
    19501 attgggattc gaaagagttt aaagacatcg ctaaaaagct tccacgttac gaaccagtgt
    19561 ttgtttattg tcagggaggc tatcgcagta atttagctgc tgaatggatg ttagaagaag
    19621 gattttctac tgtgattatt ttggataatg gaattgatgc ttggaaagat tcgggattac
    19681 cggtagaagg agaaaatgct ccaaataaaa aatcggatag ttcgtatgat taagcgattt
    19741 aattaaaaat aaaaaagcgc tgcccaataa gagcagcgct ttttttatgc gtaataatta
    19801 gcaattattt gctgattgtt actttaaatg attgttgtga ttttccatca gataaagtca
    19861 cgaagtacat tccagaactt aatgcttctg tgttgatagt taggctgttt ttaccagcaa
    19921 ctaatttaga agaagcatta ttcatgatca tacgacctga aagatctgaa atcactacag
    19981 aaacagaacc tgcattttta gctgtgaaat tgatagtaac aaaatcatta gctggatttg
    20041 gaaaaataac tgcttgatct aagtttccaa tttgagtgtt agcagataga actaatccac
    20101 taaaaacagc atcttcgaaa tcagcaccag aaagagcagg agagcttcct gcagggcgat
    20161 aatcaggagc taaataatcg taaggagtag ctagaattcc ggtagttgct actaatgaat
    20221 cgttcatgct agcagcaaac caagcgtgaa tatcgaatgt gctaccagaa gtaacttcac
    20281 acacatttcc agctgtatta cctgccaaaa tattgtgttt gaatgataat gcaccagaag
    20341 ttgcattggc ctcacaagct gttccatcga tcatcacacc tttagagtga tccatgaaaa
    20401 gtgaattgaa tattttcaaa ttagaattac gacgaatacg aactgcacga cggtatcctt
    20461 ctgcaactgt actgctaaca tttccacgaa gcggtccaac aatagtaacg ttactaaaaa
    20521 tagcagcagt aagtggtgtt aatgcatctc cggcagcatc gttatctgat tcaaatcctt
    20581 cagaagttga aacggttgga ttatcagaaa tttctggatc acgaacagct aagcagaatt
    20641 gcacatttcc gttgaagccg aagtctgtgt caaagtcatc atctaagtta cgataagcaa
    20701 ctaaatgaga acaattaact gaaccaccaa accactcaaa tgcatcatca tttgcatatg
    20761 aaacttgaac gtgattgata gttgtaccac gaccaacagc acctaatgtt aacccgttga
    20821 tttctttgtt tggagcgtaa acatatcctc caaattcgat acgtacataa gttaaagtac
    20881 cagaattatc attgtcgtca ggacttgttc caccaccaaa ttcagtatcg ctagttggag
    20941 caattccttc gatgtttgca acaccaccaa cagaattact atttcctttt cctaataaaa
    21001 ttactccacc ccaatcacct aagttccggc ttccagcagg ttgactagaa gtaaaaacaa
    21061 ctggttgaga agcagttcca tttgcattca atttagcgcc ttttgtaaca aataaaccag
    21121 aaccagaagt attaatatca ccttgaatta cagttccagc ttcgatagtt aatgtggcat
    21181 tattcgtcac ataaatctga ccttgcaaca aatacacatt accagcagtc caggttgtat
    21241 tgcttgagat attagcagat acgtttacat taggagttcc ataattggtg ttttgaggat
    21301 cccaattaca ccagccatcg gtccacatag cagcaggagc aggagcaaaa gcacctcgat
    21361 aagtaacatc cgagaaaaag ggagtttgtg cagttaaagc aaatcccatt aaaacgatta
    21421 ataaaaatgt aaacaggttt ttcatgtgtt tatttgtttg ctgcaaagct atttcagcta
    21481 tgcttcctca atttgaattc aacattacgc ttatcagcta tatccgttaa ttgattgtta
    21541 accaattttg tttcttctta atatttagat gcatttggaa tgagatacac tatttgaaaa
    21601 ttgtttattg gaattaaaaa agcctgtccg actggaaaca tcgaacaggc ttgaaataaa
    21661 ttgaatgtta acttaaaact tatacgtcac attcatagaa aatgtaggag cgaacttaga
    21721 aacccaaatc atgttatcgg aatctaaatc tagcttttga ttatcgttac gatcttggaa
    21781 gaaatagagt ttttgcgcta aaccatctcg cacgaaaaac ttcacttcta actgttgttt
    21841 caggaaactt ttgctggctt gaatatccaa tactgttcta ccattttccc aaatatctgg
    21901 ttcgttcaca ttacctacaa tagcgattcg tgtacctacc cgattcaatg ctgcagatac
    21961 tgaataacct ttatccttat tggcatatat caaccctgcg ttaaatacat aaggcgattg
    22021 tccttgtaaa ggacgtgaat catcttttgc acccagcaca tccgaaacat ccacagccga
    22081 gcgtatataa gcgtaattcg catatacggt taggttattt agaaaatgag tgtctgattt
    22141 ttttagtacc gatccaatga tggttctaaa ttcgaactct gcaccataat tatttgcttt
    22201 tgccacgttt ttgaatgata cttcattggc aacatcggcg cgggatattt gctcaattgg
    22261 attgataaat gacttataaa aaacggttgc cgaaattaat tgtccgcgac ccggataaag
    22321 ctcccaacgc acatcgtaat tatgaattaa agcacgtttt aacgtatcat taccagaata
    22381 aacgaattgc gtgttaaaat cataaaaggc aaatggtgct aactcacgaa actcaggacg
    22441 gttaagtgtt tgtgagtaac tggcgcggat attttgtttt tcagaaacag aaataactgc
    22501 atttgcagaa ggtaaaatat ctactttatt ggtacgaata gaaagaggtt tattattatc
    22561 taaagtagca tcgagcgttt gagtaaatgc ttctaaacgt gcgccccaaa ctaagcgtaa
    22621 ccatttttta tatcgtgtat ttatcatcac atacgaagca gaaagcttag atcctgccga
    22681 atatgaatca ctgttctttg tagcgtcttt caatttaaat ccaccttttc cgggagctat
    22741 taatcccata cttgtagagg cgaaaatttg atcatcggat aaatacgtta aactatcctg
    22801 aaatttaacc gatccaccac caatggttga gtattgtgta taacccaatt ggcgtgcaaa
    22861 aaactcgcga tcacgttgct ggaaagatcc tccaattttc acatcattat ttagttcttt
    22921 tccaattttt acgggataga aagcatctgc tctgaagcta taaacttgct ctttattttc
    22981 cgaaaagaac atccctccac cgtagtcagg accaacgttc gttttagaaa tattcgctcg
    23041 ccaaactgta tctcgatttg ttggttctcc cggatcattt gaatggtcca aacgtgtata
    23101 aacattccga cgtagattag gaa
//
LOCUS       DAOOQW010000016        22778 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_2757_length_22778_cov_5.395854, whole genome shotgun sequence.
ACCESSION   DAOOQW010000016 DAOOQW010000000
VERSION     DAOOQW010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 22778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22778
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2757_length_22778_cov_5.395854"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="PK281_02080"
     CDS             <1..618
                     /locus_tag="PK281_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010228914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOY48157.1"
                     /translation="GLLLSVDNSEFQLLKIVSDSVREMSEGELLQIEKARRLDIQESV
                     YFDIIRQKTATLIASCCASGAASAGATAEQVAQMKQIGEDVGIAFQIKDDLFDYESSS
                     AIGKPTGIDIKERKMTLPLIYTLANADKALKRKIINIIKNHNTDPKRVEEVIAWVKAG
                     DGIRYAEQRMAEYRDKALTALEAFPESAYRTSFINLVRYTTDRKK"
     gene            694..1110
                     /locus_tag="PK281_02085"
     CDS             694..1110
                     /locus_tag="PK281_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008270033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HOY48158.1"
                     /translation="MYPEQIVTPMREELVSAGFQELRNAQDVDQALTSTTGTTLVVVN
                     SVCGCAAGSARPGVKMAIEKAARKPEHLTTVFAGNDAEATAQARKYFAPYPPSSPAMA
                     LFKDGKLVHFIERHHIEGRTPQMIADNLLMAFEQYC"
     gene            complement(1150..1440)
                     /gene="gatC"
                     /locus_tag="PK281_02090"
     CDS             complement(1150..1440)
                     /gene="gatC"
                     /locus_tag="PK281_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HOY48159.1"
                     /translation="MKVDTQLVDELAHLSRLSYQNEAKAEIVSDLNKILAFVEKLNEI
                     NTDGVEPLIYMVDETNILRPDVARLDITQGEALKNAPKKDSDFFRVPKVIEQ"
     gene            complement(1437..2207)
                     /locus_tag="PK281_02095"
     CDS             complement(1437..2207)
                     /locus_tag="PK281_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013996193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOY48160.1"
                     /translation="MQEELIKEGEFNYIEQGNGPVLLILHGLFGALSNFRDVLDRFKD
                     DFTVVIPMMPIYGLPVQETHVRSLSAFIHRFVEFKGYKEINLLGNSLGGHVALVYVLT
                     HPENVHSMILTGSSGLYENAMGGSFPRREDYEFIRKKVEFTFYDPQTATKDLVDEVFE
                     IVNNKEKLIRILALAKSAIRHNMGDDLHTMNLPVCLIWGKNDQITPPEVADEFHRLLP
                     QSDLFWIDECGHAPMMERPTEFNNILEPWLQRLFASSK"
     gene            2492..2965
                     /locus_tag="PK281_02100"
     CDS             2492..2965
                     /locus_tag="PK281_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019975042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HOY48161.1"
                     /translation="MTNYIGEYSCKVDSKGRVMMPAGLMKQFPAALRERFVINRSVFR
                     RCLVIYPMDLWEETMNDLSKLNRFNKDNDDFIRLFTNGAVSVEVDATNRILVPRRLAE
                     FAGITSDLVFTANLNKIEIWSASGYDDVMGSYDPDSFAQLAEKVMGKLGGDAEKA"
     gene            2962..3867
                     /gene="rsmH"
                     /locus_tag="PK281_02105"
     CDS             2962..3867
                     /gene="rsmH"
                     /locus_tag="PK281_02105"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015361429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOY48162.1"
                     /translation="MSYHDPVLLHACVDGLRINPSGTYVDVTFGGGGHSREILKHLGP
                     DGRLFAFDQDNDALGNVPEDDRFTLIPHNFRYLKNYLKLYKAIPVDGILADLGVSSHQ
                     FDEAGRGFSIRFDAPLDMRMNSLQDLNAAKLLESYPEEELRRIFKEYGELNDAGKLAR
                     HLVNVRSDRPIKTTGQLMDLIRPFMRRGQEHSFAAQVFQSIRIEVNDELESLRQLLLQ
                     TVEVLKPGGRLVVMSYHSLEDRLVKNFIRSGKFSGEAEKDLFGHSHVPYQSITRKPIR
                     PDEEELEKNNRARSAVLRIAEKNEA"
     gene            3864..4268
                     /locus_tag="PK281_02110"
     CDS             3864..4268
                     /locus_tag="PK281_02110"
                     /inference="COORDINATES: protein motif:HMM:NF039650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsL-like putative cell division protein"
                     /protein_id="HOY48163.1"
                     /translation="MNEFKKKATDEKKATGAPRKQNAFTAMLLQVVDGSFLNAAGNRR
                     NLPFALFIAIIVGFYIANTYNAERTIRETDRIGNQLKEYRDEYISLKSELMFRSNQSQ
                     VAVQVAPLGLSEAKEPPHKLFIDNPDSTHSDE"
     gene            4268..6367
                     /locus_tag="PK281_02115"
     CDS             4268..6367
                     /locus_tag="PK281_02115"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HOY48164.1"
                     /translation="METSKDILWRLRLIYIVVVLFGLLILGRAFSIGVVKREFWLAKK
                     EHQTIRLNTIDAIRGNVYSTDGSLLATSVPIYNVYMDLKAEYLDKIFDKGVDSLAMMM
                     ANTFQDKTRDQYKRELRHERKKRNRYYLLRRDVRHGDLKIMKKFPILRYGRYRGGMLV
                     EVRPKREMPFRHLALRTLGRYQEGMNSIGIEGAFNEYLKGVRGKRLEQKISGGTWIPL
                     NDGAEVEPKEGNDIVSTLDIGIQDVAEAELMRQLQKHNAENGCVVLMEVETGYVRAIA
                     NLGRTGDSTYSENYNYAIGGGSEPGSTFKLASLMALFEDGLAKPTDIFDTHGGKVMFR
                     NRTMSDSHEGGFGKIPLSKALELSSNTAISQAVFRAYHANPKKFTDRIKSFHLHQALG
                     LQISGESEPIIKDPSSKTWSGTTLPWMSIGYECRLAPIQVLAFYNAVANNGKLMKPQF
                     VEAIQYRGQTVKRFDPVTIDPSICSPETIKKLKPLLEGVVERGTATNLKNPYYKIAGK
                     TGTAQIASGKSGYGEKGAKKYQASFVGYFPADNPKYSCIVVVNTTNNSVYYANLVAGP
                     VFKEVADKLYAQNIDIQEKHSTTLAIATGEKQISGYGRDISVMMNKMGIQGERPADDE
                     WVHLRVNGYKAYADKNKVNHNQVPDVRGMGLKDAVYLLENQGLRVRIIGRGLVKTQSV
                     PAGMPAQKNMEVVLELA"
     gene            6369..7832
                     /locus_tag="PK281_02120"
     CDS             6369..7832
                     /locus_tag="PK281_02120"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HOY48165.1"
                     /translation="MRLLRDILYKAGAVELHGSTNLAILSITADSRKVEKNGLFVAVK
                     GTLSDGHQFIQAAEELGAIAIVCEVMPEVRKEKVSYIVVKDSAQALGIIASNFYDNPS
                     SRIQVVGVTGTNGKTTIATLLFQLFKTLGHSCGLLSTVKNQIGDEILPSTHTTPDPVQ
                     LQFLLDKMVKAGCAYAFMEVSSHAVDQHRILGVDFAGGVFTNLTHDHLDYHKTFENYL
                     AAKQKFFTQLPADSFALVNRDDEQGLVMTEFSKAHIHTYGLSQSSDFTCRILEKELGG
                     MQLRIDDQEVWTQLIGVFNAYNLLAVYAVAVLMGQDKVNTLTALSTLRSVEGRFQYLQ
                     STDKITAIVDYAHTPDALLNVLRTINELRTGNEQVITLVGCGGDRDAAKRPEMARIAC
                     EMSDRVILTSDNPRSEEPEEIIRQMKAGVDAVNNKKVLAITDRREAIRTACALSQPGD
                     IILVAGKGHEKYQEIKGVKHPFDDMQELKETFNLQGS"
     gene            7832..9073
                     /gene="mraY"
                     /locus_tag="PK281_02125"
     CDS             7832..9073
                     /gene="mraY"
                     /locus_tag="PK281_02125"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOY48166.1"
                     /translation="MLYYLFDYLDKHTELPGVGLFQFISFRAAMALIVSLVISMVFGK
                     KIIQYLQLKQVGEIIRDLGLQGQMSKQGTPTMGGLIILSCILIPTILFAKLHNIYIIT
                     LLVSTIGLGLIGFLDDYIKVFKKNKEGLAGRFKIVGQVGVGLLVGALLYFHPDVTIRR
                     QIVGASPVVQTVDGSGMAAHNQKFVDAKAPITTIPFVKNHEFNYSKILVFLGDNYEKW
                     AWLIFIPVVIFIITAVSNGANMTDGLDGLAAGTTGIIGFVLALFAYVSGNLIFADYLN
                     IMYIPDSGEIVIYMSAFVGACVGFLWYNSYPAQIFMGDTGSLALGGIVAVVAIMLRKE
                     LLIPILCGIYLVENLSVLMQVSYFKYTKKRYGEGRRIFRMAPLHHHFQKMGFHEAKIV
                     ARFWIVGIALAVLTIVTLKLR"
     gene            9077..10417
                     /gene="murD"
                     /locus_tag="PK281_02130"
     CDS             9077..10417
                     /gene="murD"
                     /locus_tag="PK281_02130"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HOY48167.1"
                     /translation="METKQHITILGAAESGVGAAVLAKLKGWNVFVSDKGTIAPKYKD
                     ELLREEIAFEEGFHSDERVLGAELIIKSPGIPDKAPIVVEAHKRGIPVISEIEFAARY
                     TNAKLIAITGTNGKTTTTLLTYHIFKKAGLNVGVAGNVGQSFARQVAQTERDFYILEL
                     SSFQLDGMYQFKADVAVLLNITPDHLDRYDYQLQNYIFSKFRILQNMDEQGAFVYCAD
                     DPITAEALADYPTPARKFPFSLTTPQEQGAFIQENQIHFTINDPLIMSIFDLALQGKH
                     NQFNSMAAGISARVFDIRKDLVRESLMDFENIEHRLEFVTKVHGIEFINDSKATNVNS
                     TWYALESMTNPIIWIVGGVDKGNDYTMLADLVRGRVKAIVCMGKDNGKIHDAFHGMVE
                     HIVDTHGASEAVKAAYLLGKKGDTVLLSPACASFDLFENYEDRGRQFKKAVREL"
     gene            10433..11581
                     /locus_tag="PK281_02135"
     CDS             10433..11581
                     /locus_tag="PK281_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HOY48168.1"
                     /translation="MNLPSIYAKGDRTIWIVVLLLSVLSVLAVYSATGSLAYAKQGGN
                     TEHYLIKHLFLVLTGLGLMYVTHLAHYRIFSRIGQIALWVAVPLLLITLLTGTNLNEA
                     SRWITVPIINATFQTSDFAKLALIMYLARLLSRKQEQIKDFKEAFVPLMIPIFAICGL
                     ILPANFSTAAILFATSLVLLFVGRVNMKYILLLVGSGIVAFGLFIVVALNVDLPGRIG
                     TWKARIENFSSGESEANFQAEQAKIAIANGGVFGQGPGNSTQRNFLPHPYSDFIFAII
                     LEEYGFFGASILMLLYLILLYRVVRMVRYSPQAFASFLAVGCCLMLLFQAFINMAVAV
                     NLFPVTGQALPLVSMGGTSIWFTSISIGIILCVSRFCVKEKGGEDALI"
     gene            11568..12671
                     /gene="murG"
                     /locus_tag="PK281_02140"
     CDS             11568..12671
                     /gene="murG"
                     /locus_tag="PK281_02140"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019671044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HOY48169.1"
                     /translation="MRSYKFIISGGGTGGHIFPAVAIANGIRERYPNAEILFVGAKGR
                     MEMEKVPAAGYEIVGLNITGIQRSLTPKNLLVPFRLIGALNEAGKILKRFKPDVVIGV
                     GGYASGAIVFMASRKGIPTLVQEQNSYPGITNKLLAKRVKRLCVAYDGMERFFPADKI
                     RLTGNPVRQEIADQQGKREKAISHFGLNPNKQTLLVIGGSLGALSINESILAALPQIK
                     EKGLQLIWQTGKGFDGKAAAAVAELNDAGIQTYAFIKEMDLAYAAADLVVSRAGALAV
                     SELCLVKKACILVPYPFASEDHQTHNAQSLVEKGAALLVTDSDARTKLAHVALDLSSN
                     AEKREGLVKAISSLGKPDATNTIVDEVFNLMTA"
     assembly_gap    12775..12874
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            12877..14220
                     /gene="murC"
                     /locus_tag="PK281_02145"
     CDS             12877..14220
                     /gene="murC"
                     /locus_tag="PK281_02145"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HOY48170.1"
                     /translation="MGMSALGRYFLRRGIAVGGYDKTATSLTSELIAEGMNIGFVDSV
                     QKLPSELTSIADKSQVLVIYTPAIPANSEQLNYFRSQGYSLIKRAEVLGLISRNHKTL
                     AVAGTHGKTTTSTLIAHLLEQGGMHPVAFLGGISVNYNSNYLPGTTSSILVAEADEYD
                     RSFLHLHPHTAVITSTDADHLDIYGKKDALVQSFCDFADQVDTAGNLILKAGLDLPQR
                     LYDRAITYHTELPADVRLENCVAEGDKYRFDVRIQGHLLSGLTLGLPGFHNVENALAA
                     IASVYLNGLSEASIRAGLESFRGVKRRFEYILRENDFVFIDDYAHHPEELRACITSVK
                     RIYPGRHLMGVFQPHLFTRTRDFLDGFAESLSLLDEVILLDIYPARELPIEGVNSAVL
                     LDKITSAKKSLLSKDELAAYIASHKPEVLLTLGAGDIDMLVEPIRQRLTQTPVLP"
     gene            14217..15017
                     /locus_tag="PK281_02150"
     CDS             14217..15017
                     /locus_tag="PK281_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48171.1"
                     /translation="MKKGLVKMIVWGLSLLAVIVLFGFVNQQRKAAFCNENKVSILGD
                     SSVQFIVEEDVLDLLHNKGIRIDSVRAADINLLEIETEILHHPAVQSADVYYNPSGVL
                     CMDIVQRTPLIRVIDSFGESYYIDDQGNYMPLIERFTARVPVATGAINDPYYKLSMSV
                     KDIIANDSLAAGSTTDDLFTFALAAKRDTFIWAQLEQVVVHEDGDLEMVPEIGPSSVL
                     LGSTQDLEDKFKRLQLFYREGLPLVGWDAYRSLNLKYNSQIICTKNIN"
     gene            15021..16301
                     /gene="ftsA"
                     /locus_tag="PK281_02155"
     CDS             15021..16301
                     /gene="ftsA"
                     /locus_tag="PK281_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HOY48172.1"
                     /translation="MDSEIIVGLDIGTTKIVAMVGRRNEHGKIEILGHGKSTSIGVKR
                     GVVSNIERTVQSIRAAVEEAENVSGVDIRLVNVGIAGQHIKSIQHHGVLMRSNYEQEI
                     TQTDIDVLTQDMFKLVMLPGEEIIHALPQEYIIDNEKGIKDPIGMCGIRMEANFHIIT
                     GQIAAAKNIYKCVNRANLEATGLILEPLASAEAVLSKEEKEAGVALVDIGGGTTDIAI
                     FQEGIIRHTAVIALGGDIITEDIKEGCSIIKDQAEMLKIRFGSALASESKENEIVSIP
                     GLRGREPKEISLKNLANIIQARMEDIIEHIYYEIKNSGYEKKLIAGIVVTGGGAKLKH
                     VSQLIEYMTGMDTRIGYPNEHLSKATEEIASPMFATSVGLVIKGFELSDSKRVREDLK
                     QLGVKPKPQKSGGFFDAIFAKTKNFFDDDNEKNA"
     gene            16357..17814
                     /gene="ftsZ"
                     /locus_tag="PK281_02160"
     CDS             16357..17814
                     /gene="ftsZ"
                     /locus_tag="PK281_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019387915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOY48173.1"
                     /translation="MSEKKEINMTFELPQNPQNSIIKVIGVGGGGSNAVNHMFRQGIR
                     GVDFIICNTDQQALDASAIPTKVQLGLTLTEGRGAGSIPEVGRNAAIENIDDLRTILG
                     RETKMVFVTAGMGGGTGTGAAPVIASTAREMGILTVGIVTIPFTFEGKKRRKQAEDGL
                     EQLRKSVDTLLVISNDKLREIFGNLGLTAAFAEADNVLTTAAKGIAEIITVPGQINVD
                     FADVNTVMKDGGSAIMGSAIADGEGAAMRALKSALSSPLLNDSSIRGAKHILVNIAYG
                     EDIMMDEVGDLMDHMQEEAGWDANVIMGTSNDTSLGRSVCVTLVATGILTGQLEEERK
                     VLKLDEQSNTNRSHVNEVVNTPKETQTSTSTSSTLRDADEDVLIQGADRPELNFVLST
                     VSEASTSEAEESEQVRRSLERMENLKRLSIKLKTPSPVSDLESVPAFRRRNVHLQQVD
                     VSSETNVSRYTLSENPDDKKPEIRPNNTFLHDNVD"
     gene            17838..18284
                     /locus_tag="PK281_02165"
     CDS             17838..18284
                     /locus_tag="PK281_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002675428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HOY48174.1"
                     /translation="MSLEEKINADIKSAMLAKEKEKLEALRAVKSAVLLAKTEKVGAE
                     LSDDVEIKLLQKLVKQRRESALIYVQQNRPELADAETFQADIIEKYLPAQLSPEEVRV
                     VLQEIAAELGAVSVKDMGKVMGAATQKLAGRADGKLISTLVKEILS"
     gene            complement(18350..20599)
                     /locus_tag="PK281_02170"
     CDS             complement(18350..20599)
                     /locus_tag="PK281_02170"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOY48175.1"
                     /translation="MKRGEFFFPIRLLMVHFKYNILLLVFWVVLFAYVLRLWGNDFGV
                     PLLFLDPEYLGKVDFFSYLMLGASCGAFIMAFQIASYIVNSHRFPFLASVSKPFVKYT
                     INNFIIPVSFICTFLICNFQFQDKYETTTRTDIFLNSCGFVIGAGFFFLLSLTYFFSV
                     SKDIFHLFGIDILRKTDRKRVQKIQLKENVWQRISVSKKPASVIHVETYLTNPIRIAR
                     ARETAHYNPEIINQVFRQNHLAAAIFEIAVVIVMVLLGLFRDVPFFQIPAAASIFVSF
                     TMLLMLASAFHTWLKEWSIPIFIVLYLTINWLSGYEFFGKRNQAFGLDYSTEAAVIPD
                     SMLFDRQRKIQITRDTRKGLESLERWKKRAWKGDNQKPTMIFLNVSGGGLKSALWTVS
                     ALQAADSASQGHLLKNTRLITGSSGGMIGAAYMRELYLRKTSGMIDNLYDSTYTERIS
                     RDLLNAVGFSLAVSDVFLRLQRVKIGKHSYTRDRGYEFEKALNTNTGFILDKSLSAYK
                     MPEEAALTPMMIFSPTVINDGRRILISPQPISYLTSTPVKTGTILRSLPEDIEFSRFF
                     RDQDADQLRFTSAIRMSATFPYILPSVSLPSNPIMDVMDAGFRDNYGLRTSIRYLYHF
                     RKWIAANTDRVIFLQIRENHKAYDLKTHEKSTISELLMSPLGNVYENMFRIQDYQNDQ
                     LLMYADSWFDGKLEFVELDLNPPSHPSDEIISMNFHLTSLEKKRIRQAIKLPENKSAI
                     LALQKFLQN"
     gene            20957..>22778
                     /locus_tag="PK281_02175"
     CDS             20957..>22778
                     /locus_tag="PK281_02175"
                     /inference="COORDINATES: protein motif:HMM:NF012999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOY48176.1"
                     /translation="MLVLRSADVWSQGGNTCITSEANPISLPFFTNNQTLCGDGNDYT
                     GTNGCLSASGFSNPYGGQDWFYTVIPAQDGLLFVRLNDIHASGQANPVLSVYEGCPGT
                     GTCLSSVQGNNSGAVTIIPVIAGVVYFIALDALMINNFYANCYQYDLTVNLTPIVVQP
                     ACSNMGFEDGNLNSWLCTTGKSVTAPAGSPTPTYQTTNIGVVNGRHTITTGGTDPCGG
                     FPQVDPLGGSRSVMIGNNNTGAEAEMISQTFLVGASNSSFTYRYAVVFEDPGHTSAEQ
                     PFFIARLRNDQGDVIPCSEFIVSAAASLPGFFNSTTCTGVRYKPWSTVNVDLSNYMGQ
                     SVTVEFTVGDCSQGGHFGYAYVDASCSPSTLAALADTICPGQSVTLIAPSGYASFAWQ
                     PGNITTQSITVSPAVTTIYTLNLTAFNGCVTNAQVPITVAPIPVPVIQYQAPACDLPV
                     QVQSLSTISSGIIASQQWTFGGSALPSASSQSTVNVTYPGPGSYPISLQLVSDQGCTA
                     SATQTVIVPPCVFRIAITGDTICPGQCLTFPVSKAYGTPPYSYLWSNGSNDSTITLCS
                     NVSQQVSLTVTDADGFIASDTAMITVVPDIYFQPIITHVSC"
BASE COUNT         6549 a         4463 c         5103 g         6563 t
ORIGIN      
        1 ggtttgcttt tatcggtcga taattcagag tttcagttat tgaagattgt aagtgattcg
       61 gttcgggaga tgagtgaggg tgaattgcta cagattgaga aagcacgtcg attagatata
      121 caggaatcgg tttactttga tattatccgt cagaaaacag ctacattgat agcttcatgt
      181 tgtgcgagtg gagcagcttc ggcaggtgct actgcagagc aagtggcgca aatgaagcag
      241 attggtgaag atgtaggaat tgcgtttcag atcaaggacg atttgtttga ttatgagagc
      301 agtagtgcca ttggaaagcc aactggaatt gatattaaag agcgaaaaat gacacttcca
      361 ttgatttata ctttagcgaa tgctgataaa gcacttaagc ggaagattat caatatcatc
      421 aagaaccaca atacagatcc caaacgagta gaagaggtga ttgcatgggt taaggcaggt
      481 gatggaattc gttatgccga gcaacggatg gctgagtacc gcgataaggc gttaacagct
      541 ttggaggcat ttcctgaaag tgcctatcgt acatcgttca taaatttggt acgttataca
      601 accgaccgga agaagtgaca gcagaaagtg acgaagcgac cgttatttta aatttgcact
      661 acctttgaca acccaatttg aaaaacaata ctgatgtatc ccgaacaaat agtaacccca
      721 atgcgtgaag agcttgtaag cgcaggcttt caggaattac gcaatgcaca agatgttgat
      781 caggcattaa ccagcacaac aggaacaaca cttgttgttg taaattctgt atgtggatgt
      841 gcggcaggat cggctcgtcc aggtgtgaaa atggctattg aaaaagcggc tcgtaaacct
      901 gaacatttga caactgtgtt tgcaggaaac gatgcggagg caacagctca agcacgtaaa
      961 tattttgctc catatccacc atcatcacca gcaatggcat tatttaaaga tggcaaactg
     1021 gttcatttta ttgaacgtca tcatattgaa ggtagaactc ctcaaatgat tgccgacaat
     1081 cttctgatgg catttgagca atattgctaa gacttagtag aaaaaagaaa ggcgcctaaa
     1141 gcgccttttt tattgttcga ttactttcgg aactcggaaa aaatcggagt cttttttcgg
     1201 agcatttttc aatgcttctc cttgtgttat atctaagcgg gcaacatctg ggcgaaggat
     1261 atttgtttca tctaccatgt agataagtgg ttcaactccg tcggtgttga tttcattcaa
     1321 tttttctacg aaagccaaaa ttttgttgag gtcggaaacg atttcagcct tcgcttcatt
     1381 ttgataggac aagcgcgaaa gatgcgctaa ttcatcaacc aattgagtat ctactttcat
     1441 ttcgaactag caaataggcg ttgaagccaa ggttctagaa tattattaaa ttcagttggg
     1501 cgttccatca taggagcatg tccgcactca tcaatccaga ataaatccga ttgtggtaat
     1561 aatcgatgaa actcgtcggc tacttcagga ggagtaattt gatcgttttt accccagatg
     1621 aggcaaacgg gcaaattcat ggtgtgtaaa tcatcgccca tgttgtgacg aatagctgat
     1681 ttagctaatg ctaaaatccg gatgagtttt tccttgttgt taacgatttc aaacacttcg
     1741 tctactaagt ctttagtagc tgtttgagga tcataaaagg taaactctac ttttttacgg
     1801 ataaactcgt agtcttctct gcgaggaaac gatcctccca tagcattctc atacaaacca
     1861 gaacttccag taagaatcat agaatgaaca ttttcgggat gagttagcac atatactagt
     1921 gccacatgtc ctcctagaga attacctaaa aggttaatct ccttatatcc tttgaattca
     1981 acaaaacggt ggatgaaagc tgataggctt ctcacatgcg tttcctgaac aggaagtcca
     2041 taaatcggca tcataggaat aaccactgta aagtcatcct taaaccgatc gagcacatct
     2101 cggaaattac taagtgcacc gaaaagtccg tgaagaatga gcaatactgg gccatttccc
     2161 tgctctatat aattgaattc accttctttt atcagttcct cttgcattcg ttattcttta
     2221 gaaaccgacc aaaagtaacc aaattatggg aagtatatcc attgccatga aaaagcgggg
     2281 aaataggtgt gtaaaccgaa ctaaaaatag tttagttttg tcttaatgta agaaatgcct
     2341 ccacttttta ccaccgcctg aaatcccttt gtagaaagag ttttgaggtg cacaataaaa
     2401 ataaatgaaa caagttttca acaaagtggt aagatgtggt aagctgtggt tttttattct
     2461 atacttttac ctcccaaatt aaccctgtgg tatgactaat tacattgggg aatactcatg
     2521 taaggtagat tcaaaggggc gggtgatgat gcctgccggc ctgatgaagc agtttccagc
     2581 tgctttgcgg gagcgctttg tgatcaaccg aagtgtgttt cgtcgatgct tggttattta
     2641 tccgatggat ctttgggagg aaaccatgaa tgacttatcg aaactgaatc gtttcaataa
     2701 ggacaatgat gattttatcc ggctgtttac caatggagca gtaagtgtgg aggtggatgc
     2761 aaccaatcga attttggttc cacgccgtct ggcagaattt gccggaataa cttctgatct
     2821 agtgtttaca gctaatctca acaagattga gatctggtcg gcttcgggtt atgatgacgt
     2881 gatgggttca tatgatcccg acagtttcgc tcagctcgcc gaaaaagtaa tgggcaagct
     2941 tggtggtgat gcagagaaag catgagctat cacgatcccg ttcttttaca cgcttgtgta
     3001 gacggtcttc ggattaatcc ttccggcacc tatgtcgatg tcacctttgg tggtggtgga
     3061 catagtcggg agattctgaa gcatttgggt ccagatggaa ggttattcgc ctttgatcag
     3121 gacaatgatg ctttaggaaa tgttccagaa gacgatcgat ttaccctgat tccgcacaat
     3181 ttcagatacc tcaaaaacta cctgaaactg tataaggcta tcccggttga tggcattctt
     3241 gccgacttgg gagtgtcgtc gcatcaattt gatgaggcag gtagaggatt ttcgattcgg
     3301 ttcgatgccc cattggatat gcggatgaac tcgcttcagg atttgaatgc agctaaactg
     3361 ctcgaatcat atcccgaaga agagcttaga agaattttca aagaatacgg ggaattgaat
     3421 gatgcaggga aacttgctcg tcacttggtt aatgtccgct ccgaccgccc aattaaaact
     3481 actgggcaac taatggatct tattcgtccc ttcatgagac gaggacagga gcattcgttt
     3541 gctgcacaag tatttcagtc cattcggatt gaagtaaatg atgaactcga aagtttgagg
     3601 caattactgc tgcaaaccgt cgaagttctt aaaccgggag gaagattggt agtgatgagt
     3661 tatcactcct tggaggacag actggtgaag aatttcattc gaagcgggaa gttcagtggg
     3721 gaagcagaga aagatttatt cggacacagt catgttccct atcaatcgat tactcggaaa
     3781 ccaattaggc cggatgaaga agaattagaa aagaataaca gagctagaag tgcagtattg
     3841 aggattgctg aaaaaaacga agcatgaacg agtttaagaa gaaagcaaca gacgaaaaaa
     3901 aagccacagg tgctccgcgc aagcagaatg catttacggc tatgcttctt caggtagtgg
     3961 atggaagttt cctgaatgct gctggaaata gacgcaatct acctttcgca ttattcatcg
     4021 ccatcatcgt gggtttctat atcgccaata cctacaatgc agaacgaacc attcgtgaaa
     4081 ccgaccgcat tggaaatcaa ttgaaagaat accgcgacga atacatcagc cttaaatcgg
     4141 aattgatgtt tcgttccaat caaagtcagg ttgccgttca ggttgcccca ctcggactct
     4201 cagaagcaaa agaacctcct cataaattat tcatcgacaa tcccgattca acacattccg
     4261 acgagtagtg gaaacatcta aagacatact ctggaggctg cgactcattt atatagtcgt
     4321 tgtgctgttt ggcctgctca ttttggggcg ggcttttagc attggtgtgg ttaagcggga
     4381 gttttggtta gcgaaaaaag agcatcagac tattcgatta aataccatcg atgcgattcg
     4441 gggaaatgtg tattcaacag acggaagttt actcgctaca tctgttccga tttacaatgt
     4501 ttatatggat ctcaaagccg aatacttaga taagatattc gataaaggcg tggattcatt
     4561 agccatgatg atggccaata ctttccaaga caagactcga gatcaataca agcgcgagct
     4621 acgtcacgaa cggaagaaga gaaatcggta ttacttatta cgccgggatg ttcgacatgg
     4681 tgatttgaaa atcatgaaga aattccccat tctacgttat ggtcgttatc gcggtggaat
     4741 gttggttgag gtgcgtccaa aacgcgagat gccatttcgc catttggcac tgcgtacact
     4801 tggaaggtat caagaaggca tgaattctat cggaattgaa ggtgctttca atgaatactt
     4861 aaaaggtgtt cgggggaaaa gattagagca gaaaatttcg ggtggtacat ggattccatt
     4921 gaatgatgga gctgaagtag agccgaagga agggaacgac attgtatcaa ccctcgatat
     4981 tggaattcag gatgtagcag aagccgaact catgcgtcag cttcaaaagc acaatgctga
     5041 aaatggttgc gtggtgctta tggaagtgga aacgggctat gttagagcta ttgccaattt
     5101 gggaagaacc ggagatagta cttattctga aaattacaac tacgccattg gtggtggttc
     5161 cgaacctggt tctactttca agttagcttc cttaatggca ctgttcgaag atggcttggc
     5221 taaaccaact gatattttcg acactcatgg cggtaaagta atgttccgaa atcgtaccat
     5281 gagcgattct cacgaaggtg ggtttggtaa gattcctctt tcgaaagcac ttgaattatc
     5341 ttcaaatact gctatttctc aagccgtatt tagagcctat cacgccaatc caaaaaaatt
     5401 caccgatcgt attaagtcat tccacttgca tcaagcactt ggattacaaa ttagtggtga
     5461 atcagaacca attatcaaag atccgtcaag taaaacttgg tctggtacca ctttgccatg
     5521 gatgtcgatt ggatacgaat gtcgtttagc tcctattcaa gttctggcat tttacaatgc
     5581 tgtggctaac aatggtaagt tgatgaaacc tcaatttgtg gaggcaattc aataccgtgg
     5641 tcaaactgtt aaacgattcg atccagttac gattgatcca tctatctgtt cacccgaaac
     5701 aattaaaaaa ctcaagccat tattggaagg agtggttgaa cgtggaacag ccaccaactt
     5761 gaaaaatcca tattacaaaa ttgccggtaa aactgggact gctcagattg cttcaggcaa
     5821 atcgggttat ggagaaaaag gagcaaagaa atatcaagcc tcttttgttg ggtatttccc
     5881 tgccgacaat cccaagtatt cttgcattgt cgtggttaac acaacgaaca attcggttta
     5941 ctacgccaat ttggtagctg gacccgtttt caaggaagta gccgataagc tgtatgctca
     6001 aaacatcgac atacaagaaa agcattcaac aactttggct atagcaacag gtgagaaaca
     6061 gatttcgggt tacggaagag acatttccgt gatgatgaat aaaatgggaa ttcaaggtga
     6121 acgtccggct gatgatgaat gggttcacct gcgagttaat ggttataaag cttacgcaga
     6181 taaaaataaa gtcaatcaca atcaggtgcc agatgtacgt ggaatgggat tgaaagacgc
     6241 tgtttatctg ctcgaaaatc aaggattacg tgttcgcatt attggtcgcg ggttggtgaa
     6301 aactcaatct gtgcctgccg gtatgccagc acaaaaaaac atggaagtag tgctcgaact
     6361 cgcataatat gagattgctc agagacatat tatataaagc aggtgccgtt gagttacatg
     6421 gctcaaccaa tttggcaatt ctatctatca ccgccgattc gcggaaagta gagaagaatg
     6481 ggctgtttgt agctgttaaa ggcacgctgt ccgacggaca tcaatttatt caagcggccg
     6541 aagaattagg cgccattgcc atcgtgtgtg aggtgatgcc agaagtgcgc aaagaaaaag
     6601 tgagctacat cgttgtcaaa gacagcgctc aagctcttgg tatcattgct tcaaacttct
     6661 acgataatcc atcttcccgc attcaagttg ttggcgtaac aggaacaaac ggaaaaacaa
     6721 ctatcgcaac gctgttgttc cagttgttca aaacattggg tcattcctgc ggattgttat
     6781 ccacggtaaa gaaccaaata ggagacgaaa ttctaccatc aacccatact actcccgatc
     6841 cggtacaact gcaatttttg ctcgataaaa tggttaaagc gggttgtgct tatgctttta
     6901 tggaagtgag ttctcatgcg gttgatcagc accggattct tggagttgat ttcgcaggtg
     6961 gtgtgtttac caatctcaca cacgatcacc tcgattacca caaaacattt gagaattacc
     7021 ttgcagcaaa gcaaaaattc ttcacccaat tacctgccga ttcatttgca ttggttaatc
     7081 gggatgatga gcaaggattg gtgatgacgg agttttccaa agcccacatt cacacctatg
     7141 gcttaagtca aagttctgat ttcacctgtc ggattcttga aaaggaactc ggtggtatgc
     7201 agcttcgtat tgatgatcaa gaagtttgga ctcaattgat aggagtgttt aatgcctaca
     7261 atctactagc tgtttatgct gttgcggttt tgatggggca ggataaagtg aatacactca
     7321 ccgctcttag tacgcttcgc agtgtggaag ggcgttttca atacttgcaa tcgaccgata
     7381 agattactgc cattgtcgat tatgctcaca ctccagatgc cttgctgaat gtgttgcgaa
     7441 caatcaatga gctacgcact ggaaacgagc aggtaatcac cttggttggc tgtggaggcg
     7501 atcgcgatgc agctaagcgt cctgaaatgg ctcgtattgc ttgcgagatg agtgatcgag
     7561 taattctcac atccgataat ccacgttcgg aagagccaga agaaattatt cggcagatga
     7621 aagctggtgt ggatgccgtg aataacaaaa aagtattggc tatcaccgat cgtcgcgaag
     7681 ctattcgtac ggcttgtgct ttaagtcagc cgggcgatat catcctcgtt gcaggaaaag
     7741 gccacgaaaa atatcaggaa atcaaaggcg tgaaacatcc atttgatgac atgcaagaac
     7801 taaaagaaac atttaatcta cagggaagct gatgttgtac tatctctttg attatctcga
     7861 taagcacacc gaattaccgg gagttggact gtttcagttt atctctttcc gcgcagcgat
     7921 ggctttaatc gtgtcgttgg tgatttcgat ggtatttgga aagaagataa ttcaatatct
     7981 gcaactcaag caggtagggg aaattatccg tgaccttggt ttgcaaggac aaatgagtaa
     8041 acaaggaact cccacaatgg gcggtttaat tattctatcg tgcattctca ttccaacaat
     8101 tctttttgcc aaactgcata atatctatat aataaccttg ctcgtttcta caataggctt
     8161 gggcttgata ggttttttgg atgattacat taaagtattc aagaaaaata aggaaggttt
     8221 agctgggcgt ttcaagattg taggacaagt aggtgttgga ttattggttg gcgcattact
     8281 ttatttccac cccgatgtga ccattcgtcg tcagattgta ggtgcctcac cagttgttca
     8341 aactgttgat ggttcaggca tggcagctca caatcaaaag tttgtggatg caaaagcacc
     8401 aattactacc attccttttg ttaagaatca cgagttcaac tattctaaaa tcctcgtttt
     8461 ccttggagat aactacgaaa aatgggcttg gttgattttc attcctgtag tcattttcat
     8521 catcacggct gtttcgaatg gagcgaatat gacagatggt ttggatggcc ttgccgccgg
     8581 aactactggt atcattgggt ttgtactcgc cttgttcgct tacgtttcgg gtaacctcat
     8641 tttcgctgat tacctaaata tcatgtatat ccccgattcg ggagagattg taatctacat
     8701 gagcgctttc gtcggagctt gtgttggatt cctctggtat aacagctatc ccgctcaaat
     8761 attcatgggc gatacgggca gtttggcttt gggtggaatt gtagctgttg tggccatcat
     8821 gctgcgcaaa gaattgctta ttcctattct gtgtggaatc tacctcgtgg aaaacctaag
     8881 tgttctcatg caagtgagct atttcaagta cacgaagaaa cgctatggcg aaggccgaag
     8941 gattttccga atggctccgc tacatcacca ttttcaaaag atgggctttc atgaagccaa
     9001 aatcgttgcg cgcttctgga ttgtaggaat tgctcttgct gtactcacca ttgtaacctt
     9061 aaaactccgc tagtctgtgg aaacgaagca acatataacc attctgggtg ccgccgaaag
     9121 tggcgtcggt gctgctgtgc ttgctaagct caaaggctgg aatgtgttcg tttctgacaa
     9181 aggaactatt gctcctaaat acaaggatga acttcttcgc gaagaaatag cattcgagga
     9241 aggatttcat tccgatgaac gtgtattagg agctgagttg attattaaaa gtccgggtat
     9301 tcctgataaa gctcctattg tagttgaggc gcataaacgg ggcattccgg tgatttcgga
     9361 aatcgagttt gcagcacgtt ataccaatgc aaagttgatt gctataacag gcaccaatgg
     9421 taagacaacc acgactttgc tgacgtatca cattttcaaa aaagcaggtt tgaatgtagg
     9481 cgttgctgga aatgtgggac aaagttttgc tcgtcaggtt gctcaaaccg aacgtgattt
     9541 ttacatactc gaattgagca gttttcagct tgatgggatg tatcagttta aagccgatgt
     9601 ggcggtattg ctcaatatca cacccgatca cctcgaccgt tacgattatc aacttcagaa
     9661 ctacatcttt tccaagttcc gcatccttca gaatatggac gaacagggcg catttgtcta
     9721 ttgtgccgac gatcccatta cagctgaagc cttagccgat tacccaactc ctgcccggaa
     9781 gtttccgttt tcgcttacta caccacagga acaaggagca tttatccaag aaaatcaaat
     9841 tcacttcact ataaacgatc cactcattat gtccattttc gacctagccc tgcaaggcaa
     9901 acacaatcaa tttaactcga tggcagctgg aatctctgcc cgcgtgttcg atatccgcaa
     9961 agatctcgtt cgcgaaagcc tgatggattt cgaaaatatt gagcaccgtc ttgaatttgt
    10021 taccaaagtt catggtatcg agttcatcaa cgactcaaaa gcaaccaatg ttaattcaac
    10081 ttggtatgcg ctcgaaagca tgactaatcc aatcatttgg attgttggcg gagtggacaa
    10141 aggaaatgat tatacaatgc ttgccgacct tgttcgtggt cgagttaaag ctatcgtgtg
    10201 catgggtaaa gacaatggaa aaatccacga tgcatttcat ggtatggtag aacatattgt
    10261 ggatacacat ggtgcttcag aagctgtgaa agccgcgtat ttactaggta aaaaaggtga
    10321 taccgttttg ctttctccgg cttgtgccag cttcgacctt tttgagaact acgaagatcg
    10381 tggtcgccag tttaaaaagg cggttcgcga actctaatat ttcaaatcat acatgaacct
    10441 tccatcgata tacgctaaag gagaccgcac catctggatc gtggtgttgc tgctttctgt
    10501 gctttctgta ctcgctgttt acagtgctac aggatcactt gcgtatgcaa aacagggagg
    10561 aaataccgag cattatctta tcaagcattt gtttttggtg cttaccgggc tcggacttat
    10621 gtatgtgaca catttagctc attaccgaat cttctcccga ataggacaga ttgctctttg
    10681 ggtagctgtt ccacttttgt tgattacact tttgactggt actaatttga acgaagcgag
    10741 tcgctggatc acggtaccaa tcatcaatgc tacattccaa acttccgact ttgccaagct
    10801 ggctttgatc atgtatttgg cgcgtttgct ttctcggaaa caagagcaga ttaaggattt
    10861 caaagaggca tttgtccctt tgatgattcc cattttcgcg atttgtggat tgattttacc
    10921 cgcgaacttc tctactgctg ccatcttgtt tgccacatcc ttggttttgc tgtttgttgg
    10981 acgagtgaat atgaagtata tcctcttgtt ggtaggaagt ggaattgttg ctttcggttt
    11041 gtttatcgtt gttgcattaa acgttgattt gccaggaagg attggtactt ggaaagcacg
    11101 tattgagaat ttcagcagcg gtgaatcaga ggcgaatttt caagcagaac aagctaaaat
    11161 cgccattgca aacggtggtg tatttggaca aggccccgga aattctactc aacggaactt
    11221 cttgcctcac ccatattccg atttcatttt tgccatcatt ctcgaagaat acggattctt
    11281 tggtgcttcg attctgatgc ttttgtacct cattttattg tatcgagtcg tgcgaatggt
    11341 gagatatagt ccgcaagcct ttgcttcatt tttagctgtc ggatgctgtt tgatgctcct
    11401 gtttcaggca tttatcaata tggctgttgc ggtgaatttg tttccggtaa ccggacaagc
    11461 tctgcctttg gtgagtatgg gcggaacatc tatttggttt accagcattt cgataggaat
    11521 aattttatgt gttagccgtt tttgtgtaaa ggagaaagga ggagaagatg cgctcatata
    11581 agttcatcat ttcaggcgga ggcacaggcg ggcatatttt ccctgcagta gcgattgcaa
    11641 acggcattcg tgagcgatat ccaaatgcag aaattttatt tgttggtgct aaaggacgca
    11701 tggaaatgga aaaagttcct gctgctggtt atgagattgt tggattgaac attacaggaa
    11761 ttcaacgctc gttaacacct aagaacttgt tagtaccatt ccgtttgata ggtgctttga
    11821 atgaagcagg gaaaatcctc aaacgattta aacccgatgt ggtgattggt gtgggcggat
    11881 atgcaagtgg tgctatcgtt tttatggcat cgcgtaaagg cattccaacg ttggttcagg
    11941 agcagaattc atatcccgga atcaccaata agttgcttgc gaaacgagta aagcgcttgt
    12001 gtgtcgcata cgatggaatg gaacgctttt ttccagccga taaaattcgt ttaacgggta
    12061 atcctgtccg ccaggaaatt gccgaccaac aagggaaacg agaaaaagca atcagtcatt
    12121 ttgggttaaa tcctaataag caaacccttt tggttattgg tggcagtttg ggagcacttt
    12181 cgatcaatga aagtattcta gcggctttac ctcagattaa ggaaaaaggt cttcaattaa
    12241 tctggcaaac cggtaaaggt tttgacggaa aagccgcagc tgctgtagcc gaattaaatg
    12301 atgctggaat tcaaacctat gcatttataa aagagatgga tttagcttat gcagcagccg
    12361 atttggttgt ttcacgagct ggtgcactgg ctgtttcgga gctctgtttg gtgaagaaag
    12421 cctgcatttt agttccctat ccttttgctt ccgaagacca ccaaactcac aacgcacaat
    12481 cgttggtgga aaaaggggca gcattgctcg tcacagatag cgatgcacgt acgaaacttg
    12541 cacatgttgc tcttgatcta tcctctaatg ctgaaaaacg cgaaggacta gttaaagcga
    12601 taagttcatt ggggaaacct gatgcaacaa atactattgt ggatgaagtt tttaacctga
    12661 tgactgcctg atcatgaatt tgcaagctgt cacatatgtt tatttcttag gcgccggcgg
    12721 tattggcatg agcgctcttg gacgctattt tctgcgtcgg ggaattgctg tgggnnnnnn
    12781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngtattg gcatgagcgc tcttggacgc
    12901 tattttctgc gtcggggaat tgctgtgggt ggttacgaca aaacagctac ttcactcact
    12961 tcggaactca tcgccgaagg aatgaacatt gggtttgtcg attcggttca gaaacttcca
    13021 tcagaactaa cctctatcgc tgataaatcg caggtcttag tgatttacac tcctgctatt
    13081 cctgccaaca gcgaacagtt gaattatttc cgctctcaag gatattcact tatcaagcga
    13141 gccgaggttt taggattgat ttctcgtaac cataaaacac tagcggttgc tggtacgcat
    13201 ggtaaaacaa caacttcaac cttaattgcc catctacttg agcaaggtgg aatgcatccg
    13261 gttgctttct taggtggaat ttcggttaac tacaatagca attacctgcc aggaacaact
    13321 tccagcatct tggttgccga agccgatgaa tacgatcgtt cctttttgca tttacatcct
    13381 catacagccg taattacttc taccgatgcc gatcacctcg atatctatgg aaagaaagac
    13441 gcgctagttc aatcattctg cgattttgct gatcaggttg atactgctgg taatttgatt
    13501 ctcaaagctg gactcgattt acctcaacgg ctttacgatc gagctattac ctatcatacc
    13561 gaattgccgg ctgatgtgcg attagaaaat tgtgttgccg agggagataa ataccgcttt
    13621 gatgtgcgca ttcagggtca tcttttgagt ggattaacgc tcggacttcc cggattccat
    13681 aatgtagaga acgccttggc agcaattgct tcggtgtatt taaatggatt gtcggaagca
    13741 tcgattcgtg ctggattgga aagttttaga ggagttaaac gtcgtttcga atacatcctg
    13801 cgtgaaaacg actttgtatt tatcgacgac tatgcgcatc atccagaaga attgcgtgca
    13861 tgcatcactt ccgttaaacg tatctatcca ggtcgtcatt taatgggcgt gtttcaacct
    13921 catttattta ctcgaacacg cgattttcta gatggtttcg ccgagagttt atcgcttttg
    13981 gatgaagtta ttttgcttga tatctaccca gctcgtgagc ttcctatcga aggagttaat
    14041 tcagctgtct tactagataa aattacatct gccaagaaat ccttgctcag caaagacgaa
    14101 ctggctgctt atatcgcttc gcacaaacct gaagtgctac tcacgttagg agctggagat
    14161 attgatatgc tggttgaacc catccggcaa cgcctcactc aaacacctgt tctaccatga
    14221 aaaaaggact ggtgaaaatg atcgtttggg ggctgagttt attggctgtt atcgtgttgt
    14281 ttggctttgt caatcagcaa cggaaagcgg ctttttgcaa tgagaataaa gtatccattc
    14341 taggcgattc ttccgttcag tttattgtgg aggaagatgt gctggatttg ctacataata
    14401 aaggcattcg tatcgattcg gtacgtgcgg ctgatatcaa cttacttgaa atcgaaaccg
    14461 aaattctgca tcatcctgca gttcaatcgg ccgatgtgta ctacaatcct agcggagtgc
    14521 tttgtatgga tatcgtgcaa cgtacgccac ttattcgtgt cattgattcg ttcggagaaa
    14581 gctattacat cgacgatcaa ggcaactaca tgccacttat cgaacgattt actgctcgag
    14641 ttccggtggc aacaggtgct atcaacgacc catattacaa gttgagcatg agtgtaaagg
    14701 atattatcgc aaatgatagt ctcgctgcag gttctacaac cgatgatttg ttcacgtttg
    14761 ccttagctgc taaacgagat acattcattt gggctcaatt ggaacaagtg gtagttcatg
    14821 aagatggtga tttggaaatg gttccagaaa taggtccatc cagtgtgcta cttggctcta
    14881 cgcaagatct tgaagataaa ttcaaacgac tacagctgtt ctatcgcgag ggattacctc
    14941 ttgttggttg ggatgcctac cgttcgctga atctcaaata caacagtcaa attatttgta
    15001 ccaaaaacat aaactaaatc atggactcag aaatcatagt cggccttgat attggtacca
    15061 ccaagatagt tgccatggta ggtagacgga acgagcatgg aaaaatcgag attctcggcc
    15121 atgggaaatc cacttctatt ggggtgaaac gcggtgtcgt atcgaatatc gaacgtaccg
    15181 tacaaagtat acgtgctgct gtggaagaag ctgagaatgt atcgggtgtt gacattcgct
    15241 tggtgaatgt tggtattgcc ggtcaacata tcaagtccat tcagcatcac ggtgttttga
    15301 tgcgtagcaa ttacgagcaa gaaattacgc aaaccgacat tgacgtgctt acacaggata
    15361 tgtttaagct agtgatgtta ccgggagaag agattattca tgccttgccg caagaataca
    15421 tcatcgacaa tgagaaagga attaaagatc cgattgggat gtgcggaatt cggatggaag
    15481 ctaatttcca tatcatcacc ggacaaattg cagctgccaa gaacatctac aaatgtgtta
    15541 atcgcgcaaa cttagaagca accggattaa ttcttgaacc attggcttcg gctgaggcgg
    15601 ttttgagcaa ggaagagaag gaagcaggag ttgcattggt tgatattggt ggaggaacaa
    15661 ccgacattgc aattttccaa gaaggaatta ttcgtcatac agctgtaatt gctttaggtg
    15721 gagatatcat caccgaagac attaaggaag gctgttccat tataaaagat caagctgaaa
    15781 tgctcaaaat tcgatttggt tccgctcttg ccagcgaaag caaagagaat gagattgttt
    15841 ctattcctgg attacgtgga agagagccta aagaaatcag cttgaagaat ctggccaaca
    15901 tcattcaggc taggatggaa gatatcattg agcatatcta ttatgaaatc aagaattcgg
    15961 gatatgagaa gaaactcatt gccggaatcg tggtgacagg cggaggtgcc aagttgaagc
    16021 atgtatctca gttaatcgaa tacatgaccg gcatggacac acgtattgga tatcccaatg
    16081 agcacttgtc gaaagcaact gaagaaattg cttctccgat gtttgccacg tctgtagggc
    16141 tggtaatcaa aggcttcgaa ttgagcgata gcaaacgcgt tcgagaagat ttgaaacaat
    16201 taggtgtgaa acctaaacct caaaaatcgg gaggattctt cgatgccatt ttcgctaaga
    16261 cgaagaattt cttcgacgac gataacgaga agaatgctta ataattgaat acaacaacac
    16321 aacataccac aaacaacaac acaaacgaca atagccatga gtgagaagaa agagatcaac
    16381 atgaccttcg agcttcccca gaacccacaa aactctatta ttaaagtaat aggtgttggt
    16441 ggaggcggaa gcaacgcagt taaccacatg ttccgtcaag gaattcgcgg ggttgacttt
    16501 attatctgta ataccgatca gcaagcgtta gatgctagtg caatcccaac taaggtgcaa
    16561 ctaggattaa cacttactga gggccgcgga gctggttcta ttcctgaagt aggacgtaat
    16621 gctgccattg aaaatattga tgacctaaga acaatactcg gtcgtgaaac caaaatggta
    16681 ttcgtaacag ctggtatggg cggaggaact ggtacaggag cagctcctgt aattgcttct
    16741 acagcgcgtg aaatgggaat cttaacggtt ggtatcgtta caattccttt cacatttgaa
    16801 ggaaaaaaac gtcgtaagca agccgaagat ggcttagagc aattgcgtaa aagcgtagat
    16861 actttactcg tgatttcgaa cgataaactt cgtgagattt tcggtaatct aggcttaact
    16921 gctgcatttg ctgaagctga taatgtatta actacagctg caaaaggaat tgctgagatt
    16981 atcaccgttc ccggacaaat caacgtcgat tttgccgatg taaatacggt tatgaaagat
    17041 ggtggatctg ccattatggg atcagccatt gccgatggtg aaggtgcagc catgcgtgcc
    17101 ttgaaatcag cactatcttc tccattgttg aatgatagta gtatccgtgg agcgaaacac
    17161 attttggtga acatcgctta cggtgaagac atcatgatgg atgaagtagg tgatttgatg
    17221 gatcacatgc aagaagaagc tggttgggat gcaaacgtta ttatgggtac ttcgaacgat
    17281 acttcattag gtcgttcggt atgtgtaacg cttgttgcaa ctggaattct taccggacaa
    17341 ttagaagaag aacgtaaagt tttgaaacta gatgagcaaa gcaataccaa tcgctcgcat
    17401 gtgaatgagg ttgtaaatac tccaaaagaa actcaaacct ctactagtac aagctcaact
    17461 ttacgtgatg ctgatgagga tgttttaatc caaggagctg atcgtccgga gttaaacttt
    17521 gtattgagta cggtttctga ggcaagcaca agcgaagctg aagaaagcga acaagtgcgt
    17581 cgttcacttg agcgtatgga aaatttgaaa cgtttgagca ttaagcttaa aacgccatct
    17641 ccagtttctg atttggaaag tgtgcctgca ttccgtcgtc gtaatgtgca tttacaacaa
    17701 gtagatgtat catccgaaac caatgtttct cgttatacat tgagcgaaaa tccagatgat
    17761 aaaaaaccgg aaattcgtcc gaataatacc tttctacacg ataacgtaga ctgataaatt
    17821 ttttaatacc tgctatcatg agtttggaag aaaaaatcaa tgctgatatc aaatctgcaa
    17881 tgcttgctaa agagaaagaa aaactcgagg cattacgcgc agtgaaatcg gcggtactat
    17941 tggctaaaac tgagaaagtt ggagccgagt taagtgatga tgtagaaata aaattgcttc
    18001 agaaattagt taagcaacgt cgcgaatctg ctcttattta tgttcagcag aatcgtccgg
    18061 aattagctga tgctgaaacg tttcaagccg acattattga aaagtacctt cctgctcaac
    18121 tttcccctga agaggttcgt gttgtcctcc aagaaattgc tgctgaactt ggcgcagttt
    18181 ctgtaaaaga catggggaag gtgatggggg cagctaccca aaagctcgct ggtcgtgccg
    18241 atggtaaact gatctccacc ttagtaaaag aaatattaag ttagtgttag tttggttgtt
    18301 gtaattaaga cagagcccct ttttggggct ttgttgtttt atagcctttc tagttttgca
    18361 ggaatttctg aagggcaaga atggctgatt tgttttcggg tagttttatc gcctggcgga
    18421 tacgtttctt ctccaaagat gtaagatgga aattcatcga aataatttca tcacttggat
    18481 gagaaggcgg atttaaatcg agctcaacaa attccaattt cccatcaaac caactatcag
    18541 catacataag taattggtcg ttttgataat cctgaatacg aaacatgttt tcgtacacat
    18601 tcccaagtgg cgacatgagc aattctgaaa tcgtactttt ttcatgggtt ttcaagtcgt
    18661 aagctttgtg gttttccctg atttgaagaa atatcacccg atcggtattc gctgcaatcc
    18721 attttctaaa atgatacaaa tagcgaatgg atgttcgcaa gccataatta tctcggaaac
    18781 ctgcatccat aacatccata atcggattac taggcaagga cacagatggc aaaatatatg
    18841 gaaatgtggc actcatccga atggcagaag tgaaacgtag ttgatcagca tcctgatctc
    18901 taaaaaaccg actaaactca atgtcctcgg gcaaacttct taagatagta cccgttttta
    18961 ctggtgtaga agttaagtaa ctaatgggtt gtgggctgat gagaattctt cttccatcat
    19021 taattacggt aggagagaag atcatcatag gagttaaagc tgcttcctcg ggcattttat
    19081 aggcactcag acttttatcg agaatgaaac ccgtattggt attgagagcc ttttcgaatt
    19141 cataaccccg atcgcgggtg taggaatgtt ttccaatttt aacgcgttga agtctcaaga
    19201 aaacatcact aaccgccaaa gaaaatccaa ccgcattgag taaatctcta ctgattcgtt
    19261 cggtataagt gctatcgtag aggttgtcaa tcatacccga agtctttcgc aagtataact
    19321 cacgcatgta tgctgctcca atcataccgc ccgacgaacc cgttatcaaa cgtgtgttct
    19381 ttaaaagatg tccttgagaa gcagaatcag cagcttgcaa agctgaaaca gtccataatg
    19441 ctgatttcaa tccacctcca cttacgttca agaaaatcat ggttggcttt tgattatccc
    19501 ctttccatgc ccgtttcttc catctttcta atgattctaa cccttttcgt gtgtctcgtg
    19561 tgatttgaat ttttcgttgc cgatcaaaca gcatggaatc gggaatgact gctgcttcgg
    19621 ttgagtaatc gagtccaaat gcttgattcc gttttccaaa aaactcatat cctgaaagcc
    19681 aattaatggt taaatatagg acgatgaata tggggattga ccattctttc agccaggtat
    19741 gaaaagcact tgccaacatg agcaacatgg taaaactcac aaaaatgctt gctgctgcag
    19801 gtatttggaa aaatggaaca tcccgaaata gtccaagtag gaccattact atcacaactg
    19861 cgatttcgaa aatggctgca gctaagtggt tttgacggaa tacttgattg atgatttcgg
    19921 ggttgtaatg agccgtttcg cgggctcgag ctattcggat aggattagtt aagtatgttt
    19981 caacatgaat taccgatgca ggctttttag ataccgatat acgttgccag acattttcct
    20041 tcaattggat tttctggaca cgtttgcgat ctgtttttct gaggatatcg attccaaata
    20101 agtgaaagat atccttacta acactgaaga aataggttaa gctaagaagg aaaaagaaac
    20161 ctgcaccaat tacgaatccg caagaattaa ggaaaatatc ggttcgagta gttgtttcat
    20221 atttatcctg aaactgaaag ttgcagatta ggaatgtaca aatgaaactt accggaatga
    20281 tgaagttgtt gattgtgtat ttaacgaatg gcttggaaac agaggctagg aaagggaagc
    20341 gatgtgaatt tacaatgtag ctggcaattt gaaaagccat gataaatgct ccacaactag
    20401 cacctaacat taagtaggag aagaaatcaa ccttaccgag gtattctgga tctagaaata
    20461 gaagcggcac tccaaagtcg tttccccaca agcgaaggac ataagcaaac aatactaccc
    20521 aaaatacgag cagcagaata ttgtatttga aatgcaccat caagagccga atcggaaaga
    20581 agaattcccc tcttttcaaa aactccttga ttttggccat taccggctgc atgtgtcttt
    20641 agtgttttta gttcttctca aaactaatgg ataatttacc aaaagcctat ttaattgact
    20701 aataggttgt ttaatctttc tgacattgtt cattcatttg tgaaaaacac gaaaacacag
    20761 attatcagct tgttatatgt gattaaacaa ctgattaaac ttgaaactta cagcacaccc
    20821 agatatacgt aacctcattc ctagctttcc gttttaattg acaatagcca atgtcctgac
    20881 cgcatgctat acctgttact taaagcaaat ctgaatgaat tgggcttctt caatgtggag
    20941 gattatttgg gtgcttttgc ttgtacttcg gtcggcagat gtatggtcac aaggaggtaa
    21001 tacttgtatt acttctgaag caaatcctat ttctctacct ttttttacaa ataaccagac
    21061 actttgcggt gatgggaatg attatacggg tactaatggt tgtttaagtg cttctggttt
    21121 tagcaatcct tatggtggac aagattggtt ttacactgta attcctgctc aagatggttt
    21181 gctcttcgtt cgattaaatg atattcatgc aagtggacaa gctaatccgg ttttatctgt
    21241 ttatgaaggt tgcccaggaa cagggacttg tttatcatca gtccaaggaa ataactcagg
    21301 tgcagtaact attatcccgg ttatagctgg agtagtttat tttattgctc ttgatgcttt
    21361 aatgattaat aatttctatg cgaactgtta tcaatacgat ttaacagtaa acttaactcc
    21421 aatagtggtc cagcctgctt gttccaatat gggatttgaa gatggaaatc tcaattcatg
    21481 gttatgtact acaggtaaat cggttacagc tcccgcagga tcgccaactc caacttatca
    21541 aactacgaat attggagttg taaatggaag acataccata acaacaggcg gaacagatcc
    21601 atgtggtggt tttccgcagg tagatccttt aggaggttca cgatcggtga tgattggaaa
    21661 caataataca ggtgccgaag ctgaaatgat ttctcaaaca tttcttgttg gagcatctaa
    21721 ttccagtttt acttatcgat acgccgtggt ttttgaggat cccggtcata catcagccga
    21781 acaacctttc ttcattgcac gattacgaaa cgatcaaggg gatgttattc cttgttccga
    21841 atttatcgtt tctgccgctg cgagtttacc cggttttttt aattccacca cttgtacagg
    21901 tgttcgatac aaaccttgga gtacagtaaa cgtcgatcta tcgaactaca tgggacaaag
    21961 tgttacggtt gaatttaccg ttggtgattg tagtcaggga ggacactttg gttatgccta
    22021 tgtagatgca tcttgctcgc catctacctt agctgctttg gctgatacca tttgtcctgg
    22081 tcaatcggtt acccttatag ctccatcagg atatgcctca tttgcttggc agccgggaaa
    22141 tatcaccact caatccatta cagtatcacc tgctgtaaca acaatctata ccttaaatct
    22201 aacagcattt aatggttgtg tgacgaatgc acaagtacct attacagttg cacctattcc
    22261 agttccagtt attcaatacc aagctccagc ttgtgatttg cctgttcaag tacaatcgtt
    22321 aagtacgatt agttcaggaa taatagcttc tcaacagtgg acttttggag gctcagcttt
    22381 acctagcgca tcctctcaat cgaccgttaa tgttacctat ccgggcccag gttcttatcc
    22441 tatttcatta cagttggttt ctgatcaagg atgtacagcc tcagctactc aaacggtaat
    22501 tgttcctcca tgtgtttttc ggattgccat aactggagac actatttgtc cgggacaatg
    22561 tttgactttt ccagtttcaa aagcatacgg aactccacct tacagctatt tgtggtcaaa
    22621 tggtagtaac gatagtacta tcacactttg tagtaatgta tcgcagcaag ttagtttaac
    22681 agttactgat gctgacggat ttatagctag tgatacagct atgatcacgg ttgttccaga
    22741 tatttacttt caacctataa taacacatgt ttcctgtt
//
LOCUS       DAOOQW010000017        22292 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_2837_length_22292_cov_5.406440, whole genome shotgun sequence.
ACCESSION   DAOOQW010000017 DAOOQW010000000
VERSION     DAOOQW010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 22292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22292
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2837_length_22292_cov_5.406440"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2435)
                     /locus_tag="PK281_02180"
     CDS             complement(<1..2435)
                     /locus_tag="PK281_02180"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HOY48177.1"
                     /translation="MEDEVSINQNTGLSKEQVLLSAKNSGTNKLNFKKENSIVHSLKS
                     LLKEPMILLLLLASTIYFITGDQANGIFLASAILLVSFISLYQESRSREALENLKELT
                     KPLCKVIRDSQTQEIHSDDLVIGDILILEEGDSVAADAELIQLNDFTVNESILTGESF
                     AVSKELTGENRMVFQGTSIATGRALAKIIAIGNETRLGKIGKSIESIPEEKTPLEIQI
                     GHFVKKMIIAGLIVFILVWGINYYLYQSILTSLLKALTLAMSVIPEEIPMAMTTFMAL
                     GARRLMLMGIIVKQMKTVETLGSATVICTDKTGTLTENKMSLSKVFVPSQNKISDANS
                     ALSNEELELIRIAMWASEPVPFDPMEIALHEAYLKSIKPDERPNYKLIHEYLLEGNPP
                     MMTHIFENNSKVRIVAAKGAAEAILSVCPNITNVEKNNIQKSIRELASKGYRILAVSK
                     ANYEGNNFPKEQTIIKFEFVGVVAFEDPPKENIKETLEAFYQAGIQVKIVTGDNAETT
                     QAISKEIGFKGFEDSITGAELMKLKDEELKRVVTEKQIFARMFPDAKLRIINALKSNN
                     EIVAMTGDGVNDGPALKAAHIGIAMGKKGSEIAKQSASLILVEDDLSKMVDAVSMGRR
                     IYANLKKANRYIISIHIPIILTVFIPLALGWLYPTLFSPIHIIFLELIMGPTCSIVFE
                     NEPIEKNAMTQKPRPFTSTFFSWKELNISILQGLMITVGVLGIYQYAILQGNSESVTR
                     TMVFLTLVTSNILLTLINRSFYYSILQTMRLKNNLVPIIIGITILLTIALVTINPVEQ
                     FFGFSPLTLTELIP"
     gene            2663..4354
                     /locus_tag="PK281_02185"
     CDS             2663..4354
                     /locus_tag="PK281_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48178.1"
                     /translation="MKKIYTFWVVIFALTVMSCQKEIITQNEASQTVQSTPHSKSLLF
                     ETMETGTKTAYTTGSVSLTSGSWTFNDALIGNSTSDRKNGAKSARVRNSGKLTMNFDR
                     TTGAGDVIINHALYGTDAACTWQLYYSQNSGSTWTAFGSPVTTSSTSLTATTFKVNLG
                     GSIRFDIRKTDASTNRINFDDISISDFSTTNPSPSLSSISPNTKVAGSAAFTLTVTGS
                     NFVNGSTVTWNGTSLTTIFNNSTQLSATVSAALVATAGSATIAVVTPAPGGGTSTSAS
                     FTITTAPAGPKKFLFDNRHAEQAGNADWVLDQDASAARYPTPLQSNVTSSTSETYWRG
                     AISAWGIALVKLGHAVETLPSTVSITYGNSSNTQDLSNYNVFVVDEPNTLFTSAEKTA
                     ILNFVQNGGSLMMISDHTISDRNNDGWDSPEIWNDLMTNNTVQNNPFGFSIDYTDIST
                     TSTNVSTTTHPVLNGTQGTVTSVQFNNGATITINPTANPNVQGLVWNSSVTKNNNNLL
                     CAVSTFGTGRVFVLGDSSPVDDGTGNSGNVLYNGWGVYSHSKLLLNASLWCAKLQ"
     gene            4446..5228
                     /gene="ttcA"
                     /locus_tag="PK281_02190"
     CDS             4446..5228
                     /gene="ttcA"
                     /locus_tag="PK281_02190"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015480157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HOY48179.1"
                     /translation="MEATKFNQEPLTKRLRKGVWDVQAKFTLLEPDDKIMVCLSGGKD
                     SYTMLDMLLHAQMVMQHSFEIVAVNLDQKQPGFPPDILPNYFEKLGVPYRILERDTYS
                     VVTEKVPEGKTMCSLCSRLRRGILYDAAEEMGCNKLALGHHREDILETFFLNLFFSGK
                     LEAMPAKFRTDDQRHVVIRPLAFCKEADIASYAEMREFPIIPCNLCGSQENMQRKVIK
                     KMMLDWEAQYPNRKEIMMTALSNIHTSHLFDVNRYDFMEMIG"
     gene            complement(5427..5828)
                     /locus_tag="PK281_02195"
     CDS             complement(5427..5828)
                     /locus_tag="PK281_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48180.1"
                     /translation="MPNTLVREKIRKKIVQTELREELARSVKPHGQVTVHIVFPAQSD
                     GYYLRIWKSTFLYSRSSEHRSRLMHVENITIAPYWTEVLPHHTHTFTLIFEGFPADVL
                     LFDLTEEIPEPGGFHYANIVRNEQDVYWIRG"
     gene            6006..6602
                     /locus_tag="PK281_02200"
     CDS             6006..6602
                     /locus_tag="PK281_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014782508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOY48181.1"
                     /translation="MSKSTKFAAMEKTDRKSEILHVAAKLFRERGYNAVSMRDIAKEL
                     DIKAASLYNHISSKQEILELTIIRIAEAFTNGINLINSQDISVKEQLTLLVELHIKLT
                     LADPDAMACVNNDWMHLAKQELDYFIQMRNRYEDKFRSILKKGISSGEIADLNVELML
                     FSMLSTLRTLHLWYGRKKSLQPAQLKADMTRVLLQGIL"
     gene            6738..7811
                     /locus_tag="PK281_02205"
     CDS             6738..7811
                     /locus_tag="PK281_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015023418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOY48182.1"
                     /translation="MAKFFPLLISDIYKDTDECSVITLEVPEQLKREFQYKQGQHLTL
                     RAMIGNQDVRRSYSLCSSPLERKWQVAVKKIDGGLFSTYANETLKKGDTIEVMPPHGT
                     FYKDCTPSQKKNYIAFAAGSGITPILSIIKTHLASEPESTFKLFYLNRNVKSILFKED
                     IEALKNEYFNRFEIFYFLTKEHRDVPLMNGRFTSDKINTLTNKIFDPKSIDECFICGP
                     ESMIFLIRDELETAGIAKEKIHYELFTAGNSTEQQTHVKSVLDKKVEGTEVTIIDGGK
                     EFHFTMSKQFDNILDGALAAGADLPFACKGGVCSTCRCKVLEGEAEMKVNYALDESEL
                     KRNFILSCQAVPTTSRLVVDFDV"
     gene            7848..8795
                     /gene="paaA"
                     /locus_tag="PK281_02210"
     CDS             7848..8795
                     /gene="paaA"
                     /locus_tag="PK281_02210"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019037896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097266 - phenylacetyl-CoA 1,2-epoxidase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit A"
                     /protein_id="HOY48183.1"
                     /translation="MTEQENQALEDAFNLRIINDEKIEPKDWMPEKYRLTHIRQISQH
                     AHSEIVGMLPEGNWITRCPSLRRKVALLAKIQDEGGHGLYLYSAAETLGISREELIDQ
                     LHSGKAKYSSIFNYPALNWADIGAIGWLVDGAAIMNQVALINTSYGPYSRAMIRICKE
                     ESFHQRQGYEILMTLMNGTPQQKEMAQDAMNRWWWPALMMFGPADDKSPNTEQSMRWK
                     LKRKTNDELRQAFVDQTVPQANLIGLTVPDKDLKWNDTRNHYDFGPLNWDEFWQVVNG
                     HGPCNKTRLEARRKAHADGTWVRDAAMAYAEKKAVVEMV"
     gene            8956..9237
                     /gene="paaB"
                     /locus_tag="PK281_02215"
     CDS             8956..9237
                     /gene="paaB"
                     /locus_tag="PK281_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit B"
                     /protein_id="HOY48184.1"
                     /translation="MKDWPLWEVFIRSKNGLEHRHCGSLHAADAAMAMENARDVYTRR
                     NEGVSIWVVPSAQITASRPEENAELFEPAADKAYRHPTFYVLPEEVKHM"
     gene            9274..10026
                     /gene="paaC"
                     /locus_tag="PK281_02220"
     CDS             9274..10026
                     /gene="paaC"
                     /locus_tag="PK281_02220"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaC"
                     /protein_id="HOY48185.1"
                     /translation="MTNNLIEYIYGIADNDLILGQRLGELCGHGPNIETDIALTNISL
                     DLFGQVRSFYQYVASLQGANANEDTVAFLRKEFEYKNVLLVEQPNTDFGYVIARQFLF
                     DVFNHLLIGRLQDSKDETLSAIAKKGFKEVSYHVRFSSDWIRRLGDGTAESHQRIQTA
                     INDLWVYTEELFQQTDADKAMIALGIGVDVTHFKADYFKRVSEILTEATLQTPDSQYF
                     QKGGKQGNHTEHMGFILTEMQYMQRTYPNMTW"
     gene            10033..10524
                     /gene="paaJ"
                     /locus_tag="PK281_02225"
     CDS             10033..10524
                     /gene="paaJ"
                     /locus_tag="PK281_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007646415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaJ"
                     /protein_id="HOY48186.1"
                     /translation="MEQEIDSTLYHILDEVADPEIPVLSVVQMGVVRKAQFVGTGVVV
                     DITPTYSGCPAMDMMGDDIKKALQVAGYEAKVNLILSPAWSTDWITEKGRKALEGYGI
                     AAPLSPEADKAVLLGNKRMVKCPQCGSTNTVMISQFGSTACKALFKCEDCLEPFDYFK
                     CLK"
     gene            11089..11868
                     /locus_tag="PK281_02230"
     CDS             11089..11868
                     /locus_tag="PK281_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008634947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOY48187.1"
                     /translation="MESILSRIENGIGYIQFNRPDVFNSFNREMALSFQKVLDAFAHD
                     HTVRAILITGNGKAFCAGQDLKEVTTPELNPGFRAILDEHYNPIITRIRNIEKPVIAA
                     VNGVAAGAGANIALACDVVLASENASFIQAFSKIGLIPDSAGTFFLPRLVGFQKALAL
                     CLLGDKVSAVDAEKMGMIYKAIPADQFEAEVEKTALTLAQMPTKALGLTKHLFNQSLT
                     NDLHAQLKAEGDFQIQASESHDYQEGVNAFVEKRKPIFRGE"
     gene            12142..13305
                     /locus_tag="PK281_02235"
     CDS             12142..13305
                     /locus_tag="PK281_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007096634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HOY48188.1"
                     /translation="MDLHHIAVIGAGTMGSGIAQVAATAGCSVQLVDAFPEALQKAEK
                     NLQTTLAKLVEKGKISQDESVRIAGNIRYESSVSTLSNAQLVIEAIVENLEVKRKLFG
                     ELESIVSADCILASNTSSLSIASIAASCKKSNRVIGIHFFNPAPLMQLVEVIPAVQTS
                     TEVRDQITSNITAWKKTVALCKDTPGFIVNRVARPFYSESIRLYEEGAADFATIDQAM
                     KTLGFRMGPFELMDFIGNDVNYAVTESVFTSFYFDPRYKPSFTQKRLSEAGFFGRKSG
                     RGYYDYSEGAEMSKSLEDKALQELIANRVLVMLINEAADALFLNVASANDIDMAMTKG
                     VNYPKGLLAWADEKGIDWCVNQLDSLYQEYHEDRYRCSALLRRMQREGKTFFN"
     gene            13307..13711
                     /locus_tag="PK281_02240"
     CDS             13307..13711
                     /locus_tag="PK281_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013870243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HOY48189.1"
                     /translation="MTPAEIIAKMMDKDAFSQWLGIQIDHISIGKCQLSMEIRPEMLN
                     GFGIAHGGISYSIADSCLAFASNSHGRQSLSIDTSINHIEPLVVGDKIIAIAKEESLK
                     NRFSFYRVEILRGDTLVALFKGTVYRTEKIWE"
     gene            14158..14847
                     /locus_tag="PK281_02245"
     CDS             14158..14847
                     /locus_tag="PK281_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3291 domain-containing protein"
                     /protein_id="HOY48190.1"
                     /translation="MTTLTLFRYPPKNQFWAFSQMQLAHTKLMNIPGLKFYKLMGSGG
                     NGGFGLWPNWSVYAFLGIWESTEAWAQFQAENTFALEANERCSEQMTSFLHPIRTHGL
                     WNGTNPFEPISPITTNADSPIAVITRAKIRLKRLPEFLLNTSVAKSVLKNQPDLLLSI
                     GLGETPLIYQATFSIWRNLAAMQQYAYKSPEHAKVISKTRQRNWYSEELFTRFQVLRC
                     EGIWKGKKMDF"
     gene            15094..16299
                     /gene="pcaF"
                     /locus_tag="PK281_02250"
     CDS             15094..16299
                     /gene="pcaF"
                     /locus_tag="PK281_02250"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0019619 - 3,4-dihydroxybenzoate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipyl-CoA thiolase"
                     /protein_id="HOY48191.1"
                     /translation="MEAYIIDGVRTPIGSYQGTLSAVRTDDLAALVIKTVVERNPAIP
                     AADYDDVILGCANQAGEDNRNVARMAALLAGLPVTVPGETVNRLCSSGLAAIIGANRA
                     IKAGDGHVFIAGGVENMTRGPLVVSKPSSAFGTDSKMYDSSFGWRFINPKMHSMYGTD
                     AMGETAENLVDQYHISRVDQDTFALNSQLKASAAQSSGRLAKEIVPVLIPQRKGDSLS
                     FEKDEFVKPTTSLEGLAKLRPAFRKEGGTVTAGNASGLNDGAAATIIASEEAVKRYGL
                     KPLARIVSSAVVGVEPRIMGIGPVEASNRALAKAGLTMADMDIIELNEAFAAQALACT
                     RAWGLADNDPRLNPNGGSIAIGHPLGVTGARIAYSAALELQLTGKRYALITMCIGVGQ
                     GYAAIIERV"
     gene            16368..16721
                     /locus_tag="PK281_02255"
     CDS             16368..16721
                     /locus_tag="PK281_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014938953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOY48192.1"
                     /translation="MQSKFLRKIFYFGSYYTDFFNTLKPEVKKKFNWTLQLIATTQKV
                     PEKYFKHLSGTNGIYEVRVEVGSDIYRVFCFFDEGQLIILVNGFQKKTQKTPNNEITL
                     AEKLQNQYYHEKNQK"
     gene            16702..17007
                     /locus_tag="PK281_02260"
     CDS             16702..17007
                     /locus_tag="PK281_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOY48193.1"
                     /translation="MKKTKNNLTSFEDHLEDQYGKRGTALREEYEEGFEAFKFGALLQ
                     ELRKEKGLTQEELALKCGTTKNYISRIENNASDIRLSTLMRIIREGFGGQLKFNVDF"
     gene            17079..19574
                     /gene="paaZ"
                     /locus_tag="PK281_02265"
     CDS             17079..19574
                     /gene="paaZ"
                     /locus_tag="PK281_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019037911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation bifunctional
                     protein PaaZ"
                     /protein_id="HOY48194.1"
                     /translation="MNKLQNYVNGHWTDGQGEGVPLIDSVTGEVICHATAEGLNFGEI
                     LHYGRTKGGEKLRRMTFQERGNMIKKLALYLSKRKEQFYEISYRTGATRVDSWIDIEG
                     GFGNLFANASLRKLFPNQPYHVEGEAIDLSRSGRFMAHHILVPKRGVAVHINAFNFPI
                     WGMLEKCAVNWMAGVPAVVKPATSTSYLTEAVVREIIASNILPEGALQLICGSAGDML
                     DHVNDQDVVTFTGSASTGRKLKAHPRMIAESVPFNMEADSLNCSVLGLDAAPGTPEFD
                     LFISQARSEITVKAGQKCTAIRRLIVPANYIDDVQVALGKALDKVSLGDPRLKEVRMG
                     SLVSKDQVREVKERVAEIAQTASIVYGNVDALELIGADAEKGAFIAPILLREEKPFEN
                     MRVHETEAFGPVSTLMPYNTIDEAVDLARLGKGSLVSSIVTNDDHLAKDYVIQSATHH
                     GRILVLNRENAKQSTGHGSPLPLLVHGGPGRAGGGEEMGGVRGIKHYMQRCAIQGSPT
                     TLTEITGIYQPNAAYKEAPEHPFKYHWEDIQPGMSLKTHKRTVTDSDIISFANVSWDH
                     FYAHTDITSLKGSIFEKRTAHGYFILSAAAGLFVYPNKGPVAANYGLEECRFVRPIYH
                     NDTIYVRLTCKQKIERDSVGKEIPCGIVKWFVEVFDQNDELVAIATILTMVQKKSPFV
                     QITRANVEGYLAKLKENTPAAWGQLTPQHMVEHMEFTLRIAAGEIQDFEFSTPAEHLE
                     KFHDSVYNFRAMPRDFKHPLMSPDSLEPLRHADLKIAKAKLLEAWDAFNAYFKANPDA
                     QTKNMVFGMLDKFEWDLLNIKHFNHHFEQFGIL"
     gene            19673..19897
                     /locus_tag="PK281_02270"
     CDS             19673..19897
                     /locus_tag="PK281_02270"
                     /inference="COORDINATES: protein motif:HMM:NF016167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HOY48195.1"
                     /translation="MKHYSEIITIDPQIRFGKPCIRGMRISVYDILSWLASGMSTGEI
                     LQDYPDLTDEDIKASLEYAADREHKIRIAS"
     gene            19894..20235
                     /locus_tag="PK281_02275"
     CDS             19894..20235
                     /locus_tag="PK281_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018944079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HOY48196.1"
                     /translation="MKLLFDQNISFRVLNLIAEKFPESKHVKDFGLHHSSDRQIWNFA
                     KENGFHIVTYDADFYDLVTLYGHPPKVIWLRLGNTSSQNIAKKLNEYAEGVRKFIENI
                     ECKDLACLEIS"
     gene            20370..21152
                     /locus_tag="PK281_02280"
     CDS             20370..21152
                     /locus_tag="PK281_02280"
                     /inference="COORDINATES: protein motif:HMM:NF024783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HOY48197.1"
                     /translation="MKKGQVLWSKEELFLVINLYCKLSFGKMHKRNPSIIEFSNLIGR
                     TPSSVALKFGNFASFDPTLKERGIRGASNASKLDREIWDEFYFNWDEALIESEKLLAV
                     KNHSTIEELNEIDFSFFPEEGIDKERLIKTRVNQAIFRNIILASYNNCCCITGIKNSE
                     LLIASHIVPWSVDEKNRLNPMNGVCLNALHDKAFDVGMITISADDYSVIISPKFKFSE
                     SPLAIVNYFNAYESKQITLPDKFLPSKEFLKKHNSFFEKRLN"
     gene            21279..21674
                     /locus_tag="PK281_02285"
     CDS             21279..21674
                     /locus_tag="PK281_02285"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HOY48198.1"
                     /translation="MKEVEVVAAVIYFGNRILCVQRPENKYPYISKKYEFPGGKIEAN
                     ETMQEALKRELQEELNISADINELFLTVVHTYPDFELTMHSFICEVHSQELTLNEHID
                     YKWLSLSEMPQLDWAAADVPIVDKLVANG"
     gene            21667..>22292
                     /locus_tag="PK281_02290"
     CDS             21667..>22292
                     /locus_tag="PK281_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013551512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HOY48199.1"
                     /translation="MDKMLQIFTDSLQTGFIDKAILSNAEFHPELLVNQRNPPKKVLT
                     NILHEFDKCEEFYISVAFVTTGGVAAIINKLNVLKSRGVKGKLLVSQYLNFTQPEALK
                     RLMQFSNIELRIATTGNAHAKGYIFKHKQHHTIIVGSSNLTDSALSTNKEWNIKVAAL
                     DESGLVEKVLNEFTTDFENATPVSTNFITAYEEVYKKQLLFTKQQQLEA"
BASE COUNT         6837 a         4329 c         4518 g         6608 t
ORIGIN      
        1 ggaattaatt cagttaaagt caaaggagag aaaccaaaaa actgttctac gggattgatt
       61 gtaacgagtg caattgtcag taaaatggtt attccgatta tgattggaac tagattgttt
      121 tttaatcgca tagtttgaag aattgaataa taaaatgagc gattaattaa ggtcaataaa
      181 atattagacg taactaaagt taggaatacc attgtacgag ttactgattc agaatttcct
      241 tgaagaattg catattgata aattcctaga actcccacag tgatcattaa tccttgtaaa
      301 atgctaatgt ttaattcttt ccagctaaaa aatgtactag taaaaggacg aggtttttga
      361 gtcattgcgt ttttttcaat gggctcattt tcgaatacaa tagaacaggt tggacccata
      421 attaattcta ggaagattat atgaattgga gagaataatg ttggatataa ccagcctaga
      481 gccaatggaa taaatacagt tagtatgatt ggaatatgaa tagaaataat gtatcgattt
      541 gcctttttca ggttggcata aattctcctt cccatagaaa cagcatctac catttttgat
      601 aagtcgtcct ccactaatat taaggaggct gattgtttag caatttcact acctttttta
      661 cccatggcaa taccaatgtg agcagctttg agagctggtc cgtcgtttac tccatcaccg
      721 gtcatggcaa caatttcatt attcgatttt agtgcattga taattctcag ttttgcatct
      781 ggaaacatgc gggcaaaaat ctgcttttca gttacaactc gttttaattc ctcgtctttg
      841 agtttcatta attctgcccc agtaatacta tcctcaaaac ctttaaaccc aatttctttg
      901 gagattgctt gggtggtttc tgcattatcg ccggttacaa ttttcacttg aattccagct
      961 tgataaaaag cttctaaggt ttcttttata ttttcttttg gagggtcttc aaaagcaaca
     1021 acaccaacaa attcaaactt tataatagtt tgttcttttg ggaaattgtt tccttcataa
     1081 ttagctttcg aaacagccaa aattcgatag cctttagatg ctaattctcg tatagatttt
     1141 tgaatattat ttttttctac gttagttatg tttggacata cacttaatat agcttcagct
     1201 gcaccttttg ctgctacaat tcgaactttt gagttgttct caaaaatatg tgtcatcatg
     1261 ggtggattac cctctaatag atattcatga atgagtttat aattagggcg ttcgtcaggt
     1321 tttatggatt ttaaataggc ttcatgcaaa gcaatttcca tcgggtcaaa aggtacgggt
     1381 tcacttgccc acatggcgat tcgaatcaat tctaattcct catttgaaag agcggaatta
     1441 gcgtctgaaa tcttgttttg tgaaggcacg aataccttcg agagactcat tttattttcc
     1501 gttagcgtgc cagttttatc tgtacaaatt acagttgcgc tacctaaggt ttcaacagtt
     1561 ttcatttgtt tgacaataat tcccatcaac atcaatcttc gcgctccaag tgccataaag
     1621 gttgtcatgg ccataggtat ttcttccggt atcacactca ttgctaaggt gagtgctttt
     1681 aataaactgg ttaatatgga ttggtataaa taataattaa ttccccaaac caaaataaat
     1741 acgattaaac cagctatgat catttttttg acaaagtgac caatttgaat ttcaagaggt
     1801 gttttttctt ctggaataga ttctatgctt tttccaattt tacctaaacg agtttcatta
     1861 ccaatagcaa ttattttagc aagagctctt cctgttgcta ttgatgttcc ttgaaatacc
     1921 attcggtttt cacctgttag ttctttacta acggcaaaag attctcccgt taatattgat
     1981 tcatttacag tgaaatcatt taactgaatt aattccgcat cagcagcaac cgagtctcct
     2041 tcctcaagaa ttaaaatatc accaattact aagtcatcac tatgaatttc ttgcgtttgt
     2101 gaatctcgaa tgactttgca aagtggcttt gttagttctt ttaaattttc taatgcttct
     2161 ctacttctag attcttggta taatgaaata aatgaaacaa gtaaaatggc tgatgctaag
     2221 aatattccat ttgcttgatc accagtaata aaatatatag ttgaagcaag aagcaacaac
     2281 aatatcatgg gctcctttaa taaactcttt aatgaatgaa cgatagaatt ttctttctta
     2341 aagttaagtt tgttagtccc ggaatttttg gcagataata atacttgctc ttttgataat
     2401 ccagtattct gatttattga tacttcatct tccattataa acaattcaat ttcaagtact
     2461 aaaatagagc acttaagtta gtttttcagt ataattattt acataattct ttatttaaat
     2521 tgagcaggta gtgcatttaa ttataactaa ccttaaaacg aactggtgat ttattattaa
     2581 catacatttt taataattta ttttactcat aacatggcat cctagttata cattctatat
     2641 tgcacagtct taaccaaaaa acatgaagaa aatatacact ttctgggtag ttatttttgc
     2701 cctaacagtg atgtcttgtc aaaaagaaat catcacacaa aatgaagcca gtcaaacggt
     2761 tcaatctacc cctcattcaa aatccttgtt gtttgaaaca atggaaaccg gaactaaaac
     2821 tgcttacaca actgggtcag taagccttac ttctggttct tggactttca atgatgcctt
     2881 gattggaaat tctacttctg atcgtaaaaa tggtgcgaag tcggctcgtg ttcgtaattc
     2941 agggaagctt actatgaatt tcgatcgtac aacaggcgct ggtgatgtaa ttatcaacca
     3001 cgctctttat ggaacagatg cagcttgtac ttggcagttg tattattctc aaaattcagg
     3061 ttctacgtgg actgcattcg gttcacctgt aaccacatca agtacttcac ttaccgcaac
     3121 aacatttaaa gtgaatttgg gtggttctat ccgtttcgat atacgtaaaa cagatgcatc
     3181 aaccaatcgc attaactttg atgatataag tattagtgat tttagtacaa cgaatccatc
     3241 acctagttta agttccattt caccaaatac aaaagttgct ggatctgctg cttttacgct
     3301 gactgtaact ggaagtaatt tcgtgaatgg ttcaactgtg acatggaatg gaacatcatt
     3361 aacgacgata tttaataata gtactcaact tagtgctact gtatcggcag cacttgttgc
     3421 gacagctggt tctgcgacta ttgctgttgt aacacctgct ccaggcggag gtacaagtac
     3481 ttcagcatca tttacaatta ctactgctcc agctggtcct aagaaattct tattcgataa
     3541 tcgtcatgct gaacaagccg gaaatgctga ttgggtgcta gatcaagatg cttctgcagc
     3601 gcgttatccg acacctttac aaagtaatgt aacatccagt acttcagaaa cctattggag
     3661 aggagctatt tcagcttggg gaattgcatt agtgaaattg gggcatgctg ttgaaacatt
     3721 accatctacg gtttcaatta catacggaaa ttccagcaat acgcaagatt tgagtaacta
     3781 taatgtattt gttgtggatg agcctaatac gttatttact tctgctgaga aaactgcaat
     3841 tctaaacttt gtgcagaatg gtggtagttt aatgatgatt tctgatcata ctatttctga
     3901 tcgtaataat gatggttggg attctcctga aatttggaac gatttaatga caaacaatac
     3961 cgttcaaaac aatcctttcg gatttagtat cgattataca gatatatcaa ctacatcaac
     4021 gaacgtaagt acaactacac atccggtatt gaatggaaca caaggaacag ttacatcggt
     4081 gcaattcaac aatggggcta caataactat caatccaact gcaaatccaa atgtgcaagg
     4141 attggtatgg aattcaagcg taacgaaaaa caacaacaac cttttgtgtg cggtgagtac
     4201 tttcggaaca ggacgggtgt ttgtgttagg tgatagttct ccggtagatg atggaacagg
     4261 aaactcggga aatgtgcttt acaatggttg gggtgtgtat agtcactcta aattattgtt
     4321 gaatgcatct ttatggtgtg ctaaattgca ataatctaaa gtatcaaatg agtagcctgt
     4381 tttctgaaaa ggagacgggc ttttttgtgt ttatatgagc tcccgataat tattattttt
     4441 gcgccgtgga agcaactaaa ttcaatcagg agccgcttac aaaacgtttg cgaaaaggtg
     4501 tttgggatgt gcaagcgaaa ttcaccttgc tcgaaccgga cgataagata atggtctgtc
     4561 tttcgggtgg aaaagactcc tacacgatgc tcgatatgtt acttcacgca caaatggtga
     4621 tgcaacattc cttcgagatt gtagctgtaa acctcgatca aaagcaaccg ggattcccac
     4681 ccgatatttt gccgaattat tttgagaagt tgggtgttcc atatcgcatt cttgaaagag
     4741 atacctattc ggtcgtcacc gaaaaagtac ctgaaggaaa gaccatgtgc agcttgtgtt
     4801 ctcgtttaag acgcggcatt ttgtacgatg ctgccgaaga aatgggatgt aataaactcg
     4861 ctttagggca tcaccgcgaa gatattctcg agaccttctt tttaaacctc tttttctccg
     4921 gaaaactcga ggccatgcct gccaaattcc gaaccgatga ccagcgacat gtagttattc
     4981 gcccactagc attttgcaaa gaagccgata tagccagcta cgccgaaatg cgcgaatttc
     5041 ctattattcc gtgtaatctc tgcggctcac aagaaaatat gcaacgcaaa gtcattaaga
     5101 aaatgatgct cgattgggaa gctcaatatc ctaaccgaaa agaaatcatg atgacagcct
     5161 tatcgaatat ccatacctca catttattcg atgtgaatcg gtatgatttc atggaaatga
     5221 ttgggtgaaa taattattaa ttacgaatta cgaattacga attacgaatt acgaattttg
     5281 aaatataaac actagcacac ttcgacaggc tcagtgcgcg gatttaggca ctccctgagc
     5341 ttgtcgaagg gagcacacca gcaaactaac acatcagcat atcagcacat tagcacatca
     5401 gcacatcaac acaccagcac atcaccctaa cctctaatcc aatacacatc ctgttcattg
     5461 cggacaatat tggcataatg aaaaccgccc ggttcgggga tttcttcggt taaatcaaat
     5521 aataatacat cggcgggaaa gccttcaaaa atgagcgtga acgtgtgtgt gtgatgtggt
     5581 agaacttcgg tccagtaggg ggcgatggtg atattttcga catgcattaa gcgacttcga
     5641 tgctccgaac ttcgcgaata caaaaaggtg cttttccaaa ttcgaaggta atagccatcc
     5701 gattgagctg gaaaaacgat gtgtacagta acttgtccat gaggtttcac cgaccgcgct
     5761 aactcttctc gcaactcggt ttgaacaatc tttttcctaa ttttttctct gacaagtgtg
     5821 ttgggcatat gcgcaaatat atctcgaaaa tgacaagctt gattgaatgt ttaagtatat
     5881 gaaattgtca attttgcacg atttagttta gggcgcatga accgggctat cgcctatgca
     5941 cggcatttgg ctctatccct tgcgcgatta gtactaggct gcaacaattc aaaaaatcga
     6001 atcatttgtc aaaatctact aagtttgcgg caatggaaaa aaccgatcgg aaatccgaaa
     6061 tccttcatgt agcagccaag ctgtttaggg aaagaggata caatgccgtt tctatgcgcg
     6121 acattgcaaa ggaactggac attaaagcgg ccagcttgta caatcatatt tcatccaagc
     6181 aagaaatctt ggaattaacc attattcgaa tagctgaagc attcacaaat ggtatcaatt
     6241 tgataaatag ccaagatatt tccgttaaag aacaacttac tttattagtc gagctacaca
     6301 ttaagctcac cttagccgat cccgacgcca tggcgtgtgt caacaacgat tggatgcatc
     6361 ttgccaagca ggaactcgat tacttcattc aaatgcgaaa tcgatacgaa gacaagtttc
     6421 gcagcatctt aaaaaaaggt atcagttctg gcgaaattgc tgatttgaac gtcgaactca
     6481 tgcttttctc aatgctttcc accttgcgaa cacttcattt atggtatggt cgcaaaaaat
     6541 cgcttcagcc agctcaactt aaagccgaca tgacgcgtgt attactacaa ggaattttat
     6601 agaactccca taaagcttgt aaaaacagca tcttaatcaa tcttcatatt ttttttcaac
     6661 ccattgctga attgaaataa acttctaaat ttgctcaaca aactaacatt tgttagtctg
     6721 cacacccaac ccaaacaatg gctaaatttt ttcctctctt aatttctgat atttataagg
     6781 acaccgacga atgctcggtg ataacgctgg aagttcccga acaattaaaa cgagaatttc
     6841 aatacaaaca aggacagcat ttaacgcttc gtgccatgat aggtaatcag gatgtgcgcc
     6901 gttcatattc gctttgctcc agcccgctcg aacgaaagtg gcaggttgca gtaaagaaaa
     6961 tagatggtgg attattctca acctatgcga atgaaacctt gaaaaaaggc gatacgattg
     7021 aggtaatgcc acctcatggt actttttata aagactgtac tccaagccaa aagaaaaatt
     7081 acatcgcgtt tgctgctgga agtggaatta ctcccatttt atccatcatt aaaacacact
     7141 tagcatccga accagaaagt acgtttaagc ttttctattt gaatcgaaat gtaaagtcga
     7201 ttttattcaa agaagatatt gaggctttga aaaatgagta tttcaatcgg ttcgaaatat
     7261 tctattttct caccaaagaa caccgcgatg ttcctttgat gaatggtcga tttacatccg
     7321 acaaaatcaa tacgctcacc aataaaatat tcgatccaaa gtccattgac gaatgtttta
     7381 tttgcggacc agaatccatg attttcctca ttcgtgatga attggaaacg gccggtattg
     7441 ctaaggaaaa aatacactac gaattattta cagctggtaa ttctaccgaa caacaaacac
     7501 atgtaaaaag tgtgttagat aaaaaagtag aaggaacaga agtgactatt atcgatggtg
     7561 gtaaagagtt tcattttacc atgtcgaaac agttcgataa tatcctcgac ggagctctag
     7621 cagcgggtgc cgacttacca tttgcttgca aaggtggtgt ttgtagcacg tgtcgctgta
     7681 aagtcctaga aggtgaagct gaaatgaaag ttaattacgc tctggatgaa tcagaattga
     7741 aacgcaattt cattttgagt tgtcaggcag ttcccacgac ttcccgtttg gtagtcgatt
     7801 ttgatgttta attctctttt ttacggatca taaatagaac acgtaacatg acagaacagg
     7861 aaaatcaagc gctcgaagat gcattcaacc tgcgcataat taacgacgag aaaattgagc
     7921 ctaaagactg gatgccagag aaatatcgtt tgacgcatat ccgtcagatt tctcagcatg
     7981 cacattccga aattgtgggt atgttgcctg aagggaattg gattacacgt tgtccatcac
     8041 ttcgtcggaa agtagcttta ttagctaaaa ttcaagatga aggtggacat ggtttgtatc
     8101 tttacagtgc agccgaaacg cttggtattt cacgtgaaga attgattgat caattgcata
     8161 gcggtaaagc aaaatactcc agtatcttta attatccagc tttgaattgg gccgatatcg
     8221 gagccatcgg ttggttagta gatggtgctg caattatgaa tcaagtagca ttgattaata
     8281 catcatacgg tccatattct cgtgcgatga ttcgtatttg caaggaagaa agtttccacc
     8341 aacgtcaagg ctacgaaatc ctaatgacat tgatgaacgg aacgcctcag caaaaagaaa
     8401 tggctcaaga tgctatgaat cgctggtggt ggcctgcttt gatgatgttt ggtccggcag
     8461 atgataaatc tcctaacaca gaacaatcga tgcgttggaa attgaaacgc aaaaccaatg
     8521 atgaacttcg tcaagctttt gtcgaccaaa ctgttccaca agccaacctc attggattaa
     8581 ctgtaccaga taaagatttg aaatggaacg atacacgtaa tcactatgat ttcggtccac
     8641 ttaattggga tgaattctgg caagttgtaa acggacatgg tccttgtaac aaaacccgat
     8701 tggaagctcg tcgcaaagca cacgccgatg gcacttgggt tcgtgacgca gccatggctt
     8761 atgcagagaa gaaagcagtt gttgaaatgg tttgattaat tacgaattac gaattgcgaa
     8821 ttacgaatta cgaatttcga attacgaatt acgaattacg aatttgcaat ccaatcacca
     8881 gcacatcagc acaccagcac attaataaat ttcattttca aatcatcaca ttttcaaatc
     8941 ttcaaatttt ataatatgaa agactggcca ctatgggaag tttttatcag aagtaaaaac
     9001 ggacttgaac accgccactg cggtagcctt catgcggcgg atgctgcaat ggcaatggaa
     9061 aatgctcgcg atgtatatac ccgtcgcaac gagggcgtta gtatttgggt agttccttct
     9121 gcacaaataa ctgcttctcg tccggaggaa aatgccgaat tgtttgaacc agctgccgat
     9181 aaagcatatc gtcaccctac attctacgtg cttcctgaag aagttaaaca catgtaatca
     9241 ggtattacct gaatcatttt aaatcgaatg aatatgacga ataacctgat cgaatacatt
     9301 tacggcattg ccgataatga ccttattctc ggacaacgtc tcggtgaatt gtgcggacat
     9361 ggtcccaaca tcgaaaccga tatagcactc accaatatta gcctcgatct tttcgggcaa
     9421 gtacgtagct tttatcagta tgttgcttct cttcaagggg caaatgcgaa tgaagacacg
     9481 gttgcatttt tacgaaaaga atttgaatat aaaaacgttc ttctcgttga acaacccaat
     9541 accgatttcg ggtatgtaat tgctcgtcag tttttatttg atgtatttaa tcatctccta
     9601 attggtcgtt tgcaagacag taaggatgaa acgttatccg ccatagcgaa aaaaggtttc
     9661 aaggaagtat cctaccatgt tcgtttttct tccgattgga ttcgtcgctt aggtgatgga
     9721 actgccgaaa gtcatcaacg catccaaact gccatcaatg atctttgggt ttataccgaa
     9781 gaattgtttc agcaaaccga tgccgataaa gccatgatcg ctctcggtat tggagttgac
     9841 gtaacacact ttaaagcaga ttacttcaaa cgtgttagtg aaatcttaac tgaagcaact
     9901 ctccaaactc cagattccca atacttccaa aaaggaggaa aacaagggaa tcacaccgag
     9961 catatgggtt tcattctaac cgaaatgcag tacatgcaac gtacttatcc taacatgact
    10021 tggtaatata ccatggaaca agagattgat tcaactcttt atcatattct agatgaagtt
    10081 gccgaccctg agatacccgt cctgtcggtt gttcagatgg gtgtagttcg gaaagcacaa
    10141 tttgtgggaa cgggcgtggt agtagatatc acgcccactt acagcggttg tccggctatg
    10201 gacatgatgg gcgacgatat taaaaaagcc cttcaagtag ctggttacga agcaaaagtt
    10261 aatttaattc tatctcctgc ctggagcacc gattggatca ccgaaaaagg tcgaaaagcc
    10321 ctcgaaggtt atggtattgc cgctccttta tctcccgaag ccgataaagc cgtcctcctc
    10381 ggcaataaac gcatggtgaa atgtccgcaa tgcggctcta ccaatacggt aatgattagt
    10441 cagttcggct caactgcctg caaagcatta ttcaaatgtg aggattgcct agagcccttt
    10501 gattacttca agtgtttgaa gtaattcaca actcaacaac aagtttataa acaggtcggt
    10561 ggatagcttt tgaacacagt ctgtttagca tattattctc atttgtattt ttccactggg
    10621 aaggggatag tggtgggaat tccctactac aatttctttg tattgtcaag ctagaattac
    10681 aaacaatgca gttgataaac cttatcaaca ggcaagttta tcgttttttt tatttccatg
    10741 tttaggatat tgaacggcga attaattttc cactgggaag gggatagttg cgggaattcc
    10801 ctacataatt tctgatttat tgtcaagcgg gaattacaaa caatgcagtt gataaacctt
    10861 atcaacaggc aggttcatcg tttttttgat ttgtacgttg agggtataac tcagcagtgt
    10921 aattttccac tgggaagggg atagttgcgg gaattcccta catcaattta ctacatcatc
    10981 caagcaagat ttacgaacaa gtccgttgat aaagaaccta ttatggcttt caaattttcc
    11041 tcccaaatca gcaaagcttg cgtattttgc agccctcaat aaaagctcat ggaatcaatt
    11101 ctttctcgca tagaaaatgg tattggatat attcaattca atcgcccaga tgtatttaat
    11161 tcgtttaatc gcgaaatggc actttcattt caaaaagtgt tggatgcctt cgcgcacgat
    11221 catactgtta gagccattct aataacagga aacgggaaag cattttgtgc aggacaagat
    11281 ttgaaagaag taactacacc tgaattaaat ccaggattta gagccatttt ggatgaacat
    11341 tataatccaa tcattacacg tattaggaac atagaaaaac cagtaattgc cgccgttaat
    11401 ggagttgcgg cgggtgcagg agctaatatc gcccttgcat gtgatgtcgt acttgcaagt
    11461 gaaaatgcca gttttatcca agcatttagt aaaatcggat taattcccga tagcgctggt
    11521 acattctttt tacctcgatt agtgggcttt caaaaagcac ttgcactatg tctacttggc
    11581 gataaagttt cagctgttga tgcagagaaa atggggatga tttacaaagc tattcctgca
    11641 gatcaattcg aagcagaggt agaaaagacc gcacttacac ttgctcaaat gcctactaaa
    11701 gcattgggat taactaagca tttatttaat caaagtctga caaatgatct tcatgctcaa
    11761 ctcaaagcag aaggagattt tcaaattcaa gccagcgaaa gccacgatta ccaagaaggt
    11821 gtgaacgcct ttgttgaaaa acgaaagcct attttccgag gagaataatt ttataaatct
    11881 tgataattac ttctcaatta attttcaaag acaaagtaga catagaattt caatcttgcg
    11941 tgagggatag cagcgtaaag cccacagcaa ttgccgaaga ttaagcgaag gcaagcagcg
    12001 aggacttgta gcgtatagcc cgccccgaac ttgataatga agttttagaa gaaattatac
    12061 aggggcacgc cctaattcaa tcaatacttg ttatgctaaa aatgcatttc aataattaag
    12121 tttgacgttt aataaataaa aatggattta caccatattg ccgtaatagg agcaggaaca
    12181 atgggtagcg gaattgccca agtggccgca actgccggat gttctgttca attagtggat
    12241 gcctttcccg aggctttgca aaaagctgaa aagaacttgc aaacaacgct tgctaaattg
    12301 gttgaaaaag gaaaaattag ccaagatgaa agtgttagaa ttgctgggaa tattcgctat
    12361 gaaagttcgg tgtcgacctt gtcaaatgct caattggtga tagaagccat cgtggagaat
    12421 ttggaagtaa agcgcaaatt atttggtgaa ttagaaagca tcgtttccgc agattgtatt
    12481 ctcgcatcta ataccagttc actttccatc gcatctattg cagcatcgtg caaaaaatcg
    12541 aatcgcgtaa ttgggattca cttcttcaat cctgcgccac tcatgcaatt ggttgaagta
    12601 attcctgctg tgcaaacttc aaccgaagtg cgtgatcaaa taacttccaa catcacagct
    12661 tggaaaaaaa ctgtagctct gtgtaaagac acacccggat tcattgtcaa ccgtgtggcg
    12721 cgaccatttt acagcgaatc cattcgttta tatgaagaag gtgctgccga ttttgctacc
    12781 atcgatcagg ccatgaaaac gctcggattt cgcatgggac ctttcgaact tatggatttc
    12841 attggaaacg atgtgaatta tgcagtgacc gaatcagtct tcacttcctt ctatttcgat
    12901 ccacgctaca agccttcttt tactcaaaaa cgtttatctg aagctggatt ttttggtcgt
    12961 aaatcaggac gcggttacta tgattattcc gaaggtgctg aaatgtctaa atcccttgaa
    13021 gataaagcgc tacaggaact tattgccaat cgtgttttag tcatgctcat caacgaagct
    13081 gcagatgcac ttttcttgaa cgttgcttct gccaacgaca ttgatatggc catgaccaaa
    13141 ggcgttaatt atccaaaagg gttactcgct tgggccgacg aaaaaggcat cgattggtgt
    13201 gttaatcagc tcgattcact ttatcaggaa taccacgaag atcgttaccg atgcagcgca
    13261 ttactcagaa gaatgcaacg cgagggaaaa acatttttca attgatatga cgccagcaga
    13321 aattattgcc aaaatgatgg ataaggatgc tttcagccaa tggctaggca ttcagataga
    13381 tcatatttca attgggaaat gccaattatc catggaaatt cgtcccgaaa tgctcaacgg
    13441 atttggcatc gctcacggcg gcattagcta ttctatcgcc gatagctgtt tggcctttgc
    13501 ctccaattcc cacggccgac aatccctcag catcgatact tccatcaatc acatcgaacc
    13561 gcttgttgtg ggtgacaaaa tcatcgccat cgctaaagaa gaatccctca agaatcgttt
    13621 ttccttttac cgtgtcgaaa ttctgcgcgg agatacttta gtcgcccttt tcaaaggaac
    13681 cgtgtatcgt acggaaaaga tttgggaata attatagtgt gctagtgtgt taatgtgctg
    13741 atgtgctgtt ttaaagtgct agtgtgttaa tgtgctagtg cgctgtttta atgtgctggt
    13801 gtgttagcgt gcttatgtgc tgttttttga ttaaaaaatt aatggcgtgc cccttcttac
    13861 ctgcgcaata tgcttcggta agcgagacgg gtcgggcttt ctgctgcaat ttttgttcgt
    13921 gcctcacaaa agatttccgc tgcaatccct cacgcatttt cacagtatca tgaacattct
    13981 tcgtcaaatt aattctcgga gaatccaata atctgtttaa aagacatttc ccaataaatc
    14041 aaaaatacat ctgtattcat ttgaattttt aagaattgta aaacattcat ccctgtcttg
    14101 ggtttgcttt ataattcgac aatacacgat tttcctaagg agtcgaatcc ccaaattttg
    14161 actaccctaa ctttatttcg atatccacct aaaaatcaat tctgggcatt ttctcaaatg
    14221 caattagcgc atacgaaact aatgaatatc ccaggcttga aattctacaa actcatggga
    14281 agcggtggta atggaggttt cggactttgg cctaattgga gtgtgtatgc attcttggga
    14341 atttgggaat caaccgaagc ttgggcgcag tttcaagccg aaaatacctt tgcgcttgaa
    14401 gcaaatgagc gttgcagcga gcaaatgacc agctttctcc atcccattcg cacacatgga
    14461 ttatggaacg gcacaaatcc ttttgaaccc atttcgccca taacaacaaa tgccgattca
    14521 cccatcgcag ttattacacg agctaaaatc cgacttaaac gattacccga attcctacta
    14581 aatacatcgg tcgccaaaag tgtattgaaa aatcaacctg atttactact tagcatcggc
    14641 ctaggtgaaa cacctttaat ctatcaagca acattcagca tttggcgtaa cctagcggcc
    14701 atgcaacagt atgcctataa atcgcccgaa cacgccaaag tcatcagcaa aactcgccaa
    14761 cgaaattggt acagcgaaga attatttacc cgatttcaag tgttgaggtg tgaagggatt
    14821 tggaaaggta agaagatgga tttttaattt gaaaattagg gaatttgaaa atttgaagat
    14881 tgaatttact cgaagcctga gcatttgtcg ataactgagc aaaggaaatg cattaagtac
    14941 gcatattgaa ttctaacgaa aatcaaaaca ttttcaaatc ttcaaatctt caaattttca
    15001 aatcaaaaca tcctcaaatc aacacaccaa ccataaggga atcttgctaa ttttgcccca
    15061 gtataaaagg tatttcaacc ttttcatttt cctatggaag catatattat tgacggggtg
    15121 cgcactccga ttggaagtta tcaaggaacc ttatcggctg ttcgtactga cgatcttgcg
    15181 gcactggtga ttaaaaccgt ggtagaacgc aatcctgcga ttcctgctgc cgattatgac
    15241 gatgtgattt taggatgtgc taatcaggcg ggtgaagaca accgaaacgt ggcgcgtatg
    15301 gcggcattgt tagcaggttt gcctgtaaca gttccgggcg aaaccgtaaa tcgtttgtgt
    15361 agttctggat tagctgccat cattggggcg aatcgtgcaa ttaaagcagg cgatggtcac
    15421 gtgtttattg ccggtggtgt agaaaacatg acacgcggac cactcgttgt ttccaagcct
    15481 tcaagcgctt ttggaaccga ttctaaaatg tacgattcaa gctttggatg gcgtttcatc
    15541 aacccgaaaa tgcacagtat gtatggtacc gatgccatgg gtgaaacagc cgaaaacttg
    15601 gtcgatcaat atcatatttc gagagtagat caagatactt ttgcattgaa tagtcagcta
    15661 aaagcctctg ctgctcagtc ttctggacgc ttggcaaaag agattgttcc tgtgcttatt
    15721 ccgcaacgaa aaggtgattc tttatccttt gaaaaagacg aatttgtaaa acctactaca
    15781 agcctcgaag gtttggcgaa attacgtcct gcctttcgta aagaaggtgg aacagtgact
    15841 gcaggtaatg cttccggctt gaacgacggc gctgcagcaa ccataatcgc ctccgaagaa
    15901 gcggtgaaac gttacggatt aaaaccctta gcacgcattg ttagctctgc ggtggtaggt
    15961 gttgagccgc gcatcatggg cattggtccg gtagaagcca gcaaccgtgc gctcgctaaa
    16021 gcgggtttga ctatggccga tatggacatc atcgaactca acgaagcctt tgctgctcaa
    16081 gcattagcct gcacacgtgc ttggggattg gccgataatg atcctcgctt aaatccaaac
    16141 ggtggaagca tcgccattgg acatccgctc ggagtgaccg gtgcgcgcat tgcctattct
    16201 gctgccctcg aattacaatt gactggaaaa cgctacgccc tcatcaccat gtgtattggt
    16261 gttggacaag gctacgctgc aattatagaa cgcgtttgat ttttttgtaa ataattattg
    16321 cattaagttg tcttaatagg taactttgct tcgcttaatg agtttgaatg cagtcgaaat
    16381 ttcttcggaa aatattctac ttcggtagtt attacaccga tttctttaac actttaaagc
    16441 cagaggtgaa gaagaaattt aactggacat tgcagttaat cgcaacaact cagaaagtac
    16501 cagagaaata cttcaaacac ttatcaggaa ccaatgggat ttatgaggtt agggttgaag
    16561 taggttctga tatttatcgt gtattctgtt tttttgatga aggccaacta ataattctag
    16621 tgaatggatt tcagaaaaag acgcagaaaa cccctaataa tgaaattact ctagctgaga
    16681 agcttcaaaa tcaatactat catgaaaaaa accaaaaata atttgacctc ttttgaagat
    16741 catttagagg atcaatatgg taagcgcgga accgctcttc gtgaagaata tgaagaaggg
    16801 tttgaagcat ttaagtttgg cgctttactt caagaattac gaaaagaaaa aggcctaact
    16861 caagaagaat tagcgctgaa atgtggcact acaaaaaatt acatttcgag aattgaaaac
    16921 aacgcaagcg atattcgatt gtcaacactt atgcgcatca ttcgtgaagg ctttggtggt
    16981 caattgaaat tcaacgttga tttttaatcc tatagttcaa ccatcaacaa taaaataaac
    17041 ccatttcttt gaataggttt tataagcata aaaacatcat gaataagctt caaaattatg
    17101 taaacggcca ttggaccgac ggacaaggcg aaggtgttcc gctcatcgat tcggtgacgg
    17161 gtgaagtgat ttgccacgct actgccgaag gattgaattt tggcgaaatc ctgcattatg
    17221 gtcgcacgaa gggcggagag aaactgcgcc gaatgacctt tcaggaacgc gggaacatga
    17281 ttaagaaact cgcgctgtac ctgagcaaac gcaaggaaca gttttacgaa atttcctatc
    17341 gcaccggagc tacgcgtgtc gatagctgga ttgatattga aggtggtttt gggaacctgt
    17401 ttgccaatgc atccttgcgt aaattatttc ccaatcagcc ctaccatgta gaaggtgaag
    17461 cgattgacct atcacgcagt ggacgcttta tggcgcatca tattttggtt cctaaacgcg
    17521 gggttgcagt gcatatcaat gccttcaatt tccctatctg gggaatgctc gaaaaatgtg
    17581 cagtgaactg gatggcgggc gtgcctgcgg tagtgaaacc agctacatcc acttcctatt
    17641 tgaccgaagc ggtagtgcgc gaaatcatcg catccaacat ccttcccgaa ggtgcattac
    17701 agcttatttg tggttcggcg ggcgatatgc tcgatcatgt gaacgatcaa gatgtggtga
    17761 cctttaccgg ctctgcatcc accggaagaa aactcaaagc gcatccacgc atgattgctg
    17821 aatcggtgcc gtttaacatg gaagccgact cgctcaattg ctctgtttta gggctcgatg
    17881 ctgctcctgg aacgcctgaa ttcgatttgt ttatcagtca agcacgttct gaaattaccg
    17941 tgaaagcagg gcagaagtgt actgccattc gccgcttgat tgttcctgct aattacatcg
    18001 acgatgtgca agtagcactc gggaaagcac tcgataaagt gagcttgggt gatccacgct
    18061 tgaaagaagt gcgcatgggt tctttggtga gcaaagatca ggtgcgtgaa gtgaaagaac
    18121 gggtagccga aattgcacaa acagcttcca tcgtgtatgg aaatgtggat gccttggaat
    18181 tgattggtgc agacgcagaa aaaggagctt tcattgcgcc gattcttttg cgtgaggaaa
    18241 agccgttcga gaacatgcgg gtacacgaaa ccgaagcttt tggtcctgtt tctaccttga
    18301 tgccttacaa caccatcgac gaagccgttg atttggcacg tttaggaaaa ggatccttgg
    18361 tttcttctat tgtgaccaat gacgatcact tagcgaaaga ctacgtgatt caatcagcta
    18421 cgcatcacgg acgtatccta gtgctcaatc gcgaaaatgc gaaacaaagt acaggacacg
    18481 gatcgccgct tcctttgttg gtgcatggtg gtccaggacg tgccggtgga ggcgaggaga
    18541 tgggcggagt gcgcggtatt aagcattata tgcaacgctg cgctattcaa ggtagtccaa
    18601 ctacattgac cgaaattacg ggcatttatc agccgaatgc ggcctataaa gaagcgccag
    18661 aacatccgtt taaataccat tgggaagaca ttcaaccggg aatgtccttg aaaacccaca
    18721 agcgcacggt gaccgattcc gatatcatca gctttgcgaa cgtaagctgg gatcatttct
    18781 acgcgcatac cgatatcact tccctcaaag gcagcatttt cgaaaaacgt acggctcacg
    18841 gctatttcat tctttcggct gctgcgggat tattcgtgta tccgaacaaa ggtccagtgg
    18901 ctgcgaatta tggcttggaa gaatgccgtt ttgtgcgccc tatttaccat aacgacacca
    18961 tttacgtacg tttaacctgt aagcagaaaa ttgaacgcga cagtgtgggt aaagaaatcc
    19021 cttgcggtat tgtgaaatgg tttgtggaag tgttcgatca aaacgacgag ctcgtagcca
    19081 tcgccaccat tttgaccatg gtgcaaaaga aatcaccatt tgtgcaaatt acccgcgcca
    19141 atgtggaagg ttacctagct aaattgaagg aaaacacacc tgctgcttgg ggccaattaa
    19201 cgccgcaaca catggtagag cacatggaat ttaccttgcg tattgctgcg ggcgaaatcc
    19261 aagattttga attctctact ccagctgaac acctcgagaa attccacgat tcggtgtata
    19321 atttcagagc catgccgcgc gatttcaagc atccgctcat gtctccagat agcctcgaac
    19381 cactccgcca cgccgacctc aaaattgcta aagccaaact cctcgaagcc tgggatgcct
    19441 ttaacgccta cttcaaagcc aaccccgacg cccaaaccaa gaacatggtc tttggtatgc
    19501 tcgacaaatt cgaatgggac ctgctcaaca tcaagcattt caaccatcat tttgagcagt
    19561 ttgggatttt gtagtatagt gtactgagta tagagtatta agaggactta gtacttttta
    19621 cttaatactt tgtacataat acagtctttt gaatttttct atctttatca gtatgaagca
    19681 ttattcagaa ataataacca ttgatcctca gattcgcttt gggaagcctt gcattagagg
    19741 catgaggatt tctgtatatg atatcttaag ttggttggca tcaggaatgt ctacaggaga
    19801 aatactacaa gactaccctg atcttacaga cgaggatatt aaagcatcac ttgaatatgc
    19861 tgcagacaga gagcataaaa ttagaattgc atcgtgaaat tgctttttga tcaaaacatt
    19921 tcttttcgtg ttttaaactt gattgctgaa aagtttccag aatcaaagca tgtgaaagac
    19981 tttggattac atcattcaag tgaccgtcaa atatggaatt ttgcaaaaga aaatggcttt
    20041 catattgtaa catatgatgc tgatttctat gatttagtca cattatacgg acatcctcct
    20101 aaagttatct ggcttcgctt agggaacact tcttcacaga atatcgctaa gaaactaaat
    20161 gaatatgcag aaggggtaag gaaatttatt gaaaacattg aatgtaaaga ccttgcttgt
    20221 ttagagatta gttgatttac ttatagtatg tttcaatgag tttaaatatg aatatatact
    20281 tctgagaatt ttctatattg attaaacagc ttttaatgaa ctttgagatg gttgatatta
    20341 attatgtgaa caaaggatat aattaattta tgaaaaaagg tcaggtactt tggtcaaaag
    20401 aagaactttt tttagtaatt aatctttatt gcaaactctc ttttgggaag atgcataaaa
    20461 gaaatccttc aataattgag ttttcaaatt taattggtag gactccttcc tcagtcgctt
    20521 taaaatttgg caacttcgct agctttgatc ctactttgaa agaaagaggt attagaggcg
    20581 cttcaaatgc aagtaaactt gatagggaaa tttgggatga gttttatttc aattgggatg
    20641 aagcacttat agagagtgag aaattgttag ctgttaaaaa tcactcaact atcgaggaat
    20701 taaatgaaat tgattttagt ttttttccag aggaagggat tgataaagaa aggctaataa
    20761 aaactagagt taatcaagca atttttcgaa atattattct tgctagttat aataactgtt
    20821 gttgcataac aggtattaaa aactctgagt tattaattgc aagtcacatt gttccttgga
    20881 gtgttgatga aaagaataga ttaaatccaa tgaatggagt ttgtttgaat gctttacatg
    20941 acaaagcatt tgatgttgga atgattacta tatctgctga tgactattca gttattattt
    21001 ctccaaaatt caaatttagt gaatcgccat tagctatagt taactatttt aatgcttatg
    21061 aaagcaagca aattacttta cccgacaagt ttttaccctc caaagaattt cttaaaaaac
    21121 ataattcatt ttttgaaaaa cgattgaatt agtatagata ttttcataga atagaattgt
    21181 ttcaaattta gtctggaaaa taaatcaatt atggtagttc ttttaaatta agtaatgcag
    21241 tttacttagt ttagtaaatt attttgagtt ggaaaagtat gaaagaagta gaagttgtag
    21301 ctgctgttat ctattttgga aatcgtattt tatgtgttca aagacctgag aataagtacc
    21361 cttatatttc aaagaagtat gagtttccag gtgggaagat agaagccaac gaaaccatgc
    21421 aagaagcctt gaaacgggaa ttgcaggaag agctcaacat tagcgctgat attaatgagc
    21481 tgtttttaac ggtggtgcat acttatcctg attttgaatt gaccatgcat agtttcattt
    21541 gtgaagtgca ttctcaagag ctaacactta acgaacacat cgattacaaa tggttatccc
    21601 tttcggaaat gccacaactc gattgggcgg ctgccgatgt tcctatcgtg gataaattag
    21661 ttgcgaatgg ataagatgct tcagattttc acggatagtc ttcaaacggg ttttatagat
    21721 aaggctattc tttcgaatgc tgaatttcac ccagagctct tggtgaatca acgaaatcct
    21781 ccaaagaaag tcctaacgaa tattctacat gagtttgata aatgcgagga attttacatc
    21841 tctgttgcct ttgttactac tggtggtgtt gctgcaatta tcaataaatt aaatgtatta
    21901 aagagtcgag gagtaaaagg gaagctgttg gtttcacagt acctcaattt cacgcaaccg
    21961 gaagcgttaa agcgtttaat gcaattttcg aatattgaat tacgcattgc aaccactgga
    22021 aatgcccatg caaaagggta tatattcaag cataagcaac atcatacaat cattgttggt
    22081 agcagtaact taacagacag tgctttatca accaataagg aatggaatat caaagttgct
    22141 gcattagatg agagtggctt ggttgagaaa gtgctcaatg aatttactac cgattttgag
    22201 aatgcaacac ctgtatcaac caacttcata actgcttatg aagaggtata taagaaacaa
    22261 ttactcttta caaagcagca gcaattagaa gc
//
LOCUS       DAOOQW010000018        22143 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_2868_length_22143_cov_5.962785, whole genome shotgun sequence.
ACCESSION   DAOOQW010000018 DAOOQW010000000
VERSION     DAOOQW010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 22143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22143
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2868_length_22143_cov_5.962785"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(17..484)
                     /locus_tag="PK281_02295"
     CDS             complement(17..484)
                     /locus_tag="PK281_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009414379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HOY48200.1"
                     /translation="MSSETNPFQFEEANDSSGFLLWQVTSLWQREIRKVLEPFDLTHS
                     QFVLLASMHWLTLEKKEVTQIILSQHTKIDPMTTSTVLRTLQRKDLIERKEHSTDTRA
                     KSVALTPAGKTKVLQTVKIVEAFDDEFFAILGKEIPEFNQKLLRLLVPRMIKS"
     gene            complement(474..905)
                     /locus_tag="PK281_02300"
     CDS             complement(474..905)
                     /locus_tag="PK281_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002668908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HOY48201.1"
                     /translation="MRAQKFFIGAVSRDHTKRGESGGFMQVCHGKQAPLKRMQKGDWI
                     IFYSSKISMQSEEKCQAFTAIGQACDADVYAFEMSPEFVPFRRNVSFYPCEETAISSL
                     IPHLEFIPDKKHWGYPFRFGFFEIGEKDFRLIASHMLPNEF"
     gene            complement(902..1321)
                     /locus_tag="PK281_02305"
     CDS             complement(902..1321)
                     /locus_tag="PK281_02305"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HOY48202.1"
                     /translation="MWKGNATIITQKATINQLWNTLTDIQNWNTWDHDISWTSIKGPV
                     KQGAEFYLKPKDGPKTKLCVLEYTAPNVFSDVAYLPLAKMRTTHRFTQTEQGIEINIE
                     VEVRGLLSFLWSRIIAQNQIKGAEQQTQNLIDKAHMQ"
     gene            1485..2441
                     /locus_tag="PK281_02310"
     CDS             1485..2441
                     /locus_tag="PK281_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015362416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOY48203.1"
                     /translation="MPSSTDYILVIGSSGQIGTELVARLREIHGSSQVIAADLNIPAE
                     SDGPFEQLNVLDTQRLSEILAKYRISEVYLLAAMLSATGEKFPMKAWDLNMNGLFHLL
                     DAAKEGKIKKLYWPSSIAVFGPTTPRIETPQNTIAEPTTVYGISKLAGERWCEYYHQK
                     FGVDVRSLRYPGLIGWKSAPGGGTTDYAVHIFHEAIEKGSYTSFLSANTRLPMMYMPD
                     GINATIQIMQAPAEQIRIRSSYNLAAMSFTPDNLAAEIRKHIPNFRIDYAPDFRQAIA
                     DSWPASINDSDARKDWNWNPEFDLAKMTEDMLVNLRAMKTSV"
     gene            2585..4096
                     /gene="cysS"
                     /locus_tag="PK281_02315"
     CDS             2585..4096
                     /gene="cysS"
                     /locus_tag="PK281_02315"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010522100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOY48204.1"
                     /translation="MNTQDLFIYNTLSRQKERFEPINAPFVGLYVCGPTVYGEAHLGH
                     ARPYVTFDVLGRYLSSIGYKVRYVRNITDVGHLENDADEGEDKIAKKARLEKLEPMEI
                     VELYTNSFHESMRMLNNNHPSIEPRASGHIPEQIEMIQKIIENGFAYESNGSVYFDVI
                     KYSKTNDYGKLSGRVLEDLIAGAGNDRRVLEGQEEKHNPEDFALWKKADAYHIMRWSS
                     PWSDGFPGWHIECSAMSRKYLGETFDIHGGGMDLVFPHHESEIAQSKGSCGHAPVKYW
                     MHNNMITINGRKMGKSLGNFITLKEFFTGSSALLDQAYSPMTIRFFILQAHYRSTLDF
                     SNDALKAAEKAYKRIINGLRSLRQLDYSSDGGTIDADLKIRIEAAVVQAYAGMNDDMN
                     TAVALASLFDLLKVINSLKGGQIAMSALSKDTFDLMKSTYIHFVENIFGLLEEKGKSD
                     DTLGTLMNVILGDYVDAKSRKDYATVDRIRAQVKLEGLVIKDSKTGISWDYEE"
     gene            4086..5081
                     /locus_tag="PK281_02320"
     CDS             4086..5081
                     /locus_tag="PK281_02320"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOY48205.1"
                     /translation="MKNKWLGASLLLMAGIWTSCGGPSSSGDQPVPTETPKQAKLVPA
                     DLPAFNADTAYAITAKQVAFGPRVPNTEAHVKCADYLIATLKRYGAEVVVQSGKVTAY
                     TGESLSMRNILARVNPSTSTRIMLCAHWDTRPFSDQDIMKPKARFDGAVDGAASVGIL
                     LEIARVLQAHPAPIGVDIVLFDAEDYGNPKESNTYCLGSQYFATNPPIPNFSPKYGIL
                     LDMVGASGATFYREGFSMQYAPDVMNRVWSIGAELGYSSYFRNDPCGSVTDDHYFVNT
                     ILGIPTIDIIHHDPQKGFGDYWHTQQDNMNAVDKTTLGVVGKTLLGVVYSEKSGI"
     gene            complement(5153..6577)
                     /locus_tag="PK281_02325"
     CDS             complement(5153..6577)
                     /locus_tag="PK281_02325"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014158939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HOY48206.1"
                     /translation="MSDITEKREDEFFQDVLKYFDRAAKFVDVSAGLLDQIKYCNSVY
                     RMKFPVKMGDGYQVIEAYRVEHSHHKLPTKGGIRFSTAVNQDEVMALAALMTYKCAIV
                     DVPFGGAKGGIKISPRKLSADEMERITRRYTSELIKKNFIGPGIDVPAPDYGSGEREM
                     AWIVDTYATFFPNELNAMACVTGKPVSQGGIRGRKEATGLGVYFGIREALSNVEDCKA
                     WGLTPGMAGKTFVVQGFGNVGFHSAKFVHEGGGKIISIAEWDGAIYSADGIDPHALEA
                     HRNKTGSILNFPGTTNVDKYAALEIECDVLIPAALENQINGTNADRIKCKIIGEAANG
                     PVTPDAEAILNAKGVYIIPDIYLNAGGVTVSYFEWLKNLSHVRFGRLGKRWDEAAGHN
                     TISVLEEMTGRIATDAQKKLIERGADELDLVYSGLEETMINSYNAIRDELKSGSGIPD
                     LRTAAFVVAIKKIVQSYNTLGVFP"
     gene            complement(6774..7295)
                     /locus_tag="PK281_02330"
     CDS             complement(6774..7295)
                     /locus_tag="PK281_02330"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HOY48207.1"
                     /translation="MKTKKFAHESIAVSSEIVLPNDTNTLGNLMGGKLLHWMDIIAAI
                     SAHRHCKRIVVTASVNNVSFDRPIRLGDIVTLEAHVSRAFSSSMEVFIDVWVENHSTG
                     ERVRHNEAIYTFVAVDQLGNPIAVPELVPETELQKSRYEGALRRRQLSLILAGKMKPD
                     EATELKALFAPQG"
     gene            7438..7971
                     /locus_tag="PK281_02335"
     CDS             7438..7971
                     /locus_tag="PK281_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003443752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HOY48208.1"
                     /translation="MYLYLKAIHIIFIVTWFAGLFYMPRLLIYDVEAREKAEPGRSIL
                     VEQFRIMQKRLWFGITWPSFVLTMILGPWLVIETGYPWTTSWLVVKLGLVAGLVGYHF
                     SLQVIFQQLGKDVVKYTSNQLRLWNEVATLFLVSIVFLVVIKNELDMLYGLAGLIGLI
                     AILMLAIRIYKRIRNAG"
     gene            complement(7972..8706)
                     /locus_tag="PK281_02340"
     CDS             complement(7972..8706)
                     /locus_tag="PK281_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48209.1"
                     /translation="MIFRFLFLAIALGFSPLAQSQTSKPAKPPVKPASTGPVATQEKV
                     YRSAMELNDLEQATSAVFQILAINPALTNWKDTLCLLYHGRGLYYQSHKLASEILQKK
                     PDDVTLHEVRAQALEGLGRYPESLSDYEFLIAKSNLSIFRYKTAALQYMLQRFGECEK
                     NIDAIIANPNSDKEVVGLQVEVNGTAQMQEVPVKAAALNIRATIAIDMKRYTEAEIAL
                     NEALKIYPDFGMALQNMERLKSVAQP"
     gene            complement(8706..9689)
                     /locus_tag="PK281_02345"
     CDS             complement(8706..9689)
                     /locus_tag="PK281_02345"
                     /inference="COORDINATES: protein motif:HMM:NF036727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOY48210.1"
                     /translation="MDHSEFSKRNVQVSSLVAELLHVHDCIILPGFGGLIGNYAPARV
                     HPVTHVFQAPSKQLVFNRSLKTDDGLLVNEYARVSGLSFSLSRLYLSEYVEKLNANLL
                     AGERIEWTQIGTFNTDVERNIQFKATQSVNYLPESIGLYAIQVQPINRFPVPERRPEP
                     VFVNRELVPPVKVSRKFPVRRVLQAIPVLLIAAFIGVNASLPEGKGISTADLNIFSQS
                     ASISASALSVQSPEALPAARKSIPAPVAELFSAESAQIFIVAGCYSTEVNANGMVDYL
                     SEKGFSSYVLDRTPAGLYRVVYGNYPSIQEATDELTSIRKGLNEEAWLLIR"
     gene            complement(9716..10474)
                     /gene="kdsB"
                     /locus_tag="PK281_02350"
     CDS             complement(9716..10474)
                     /gene="kdsB"
                     /locus_tag="PK281_02350"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HOY48211.1"
                     /translation="MQTVQFIGIIPARYASSRFPGKPLVEISGKTMIRRVVEQALQSK
                     MLSRVIVATDDERIEAEVRYFGGEVVMTSPEHPSGTDRCAEVVRILGSAVHSNAVIIN
                     IQGDEPFIDPGQIDVLCGCFSNEQVELGTLVRRFSYGEDVLNPNTIKVVRDIHGYGIY
                     FSRLPIPFERDAPQGKLPDLTAHFQHIGLYGYRAATLLHVASLAPSSLELTEKLEQLR
                     WIENGYRIMTALSEHESWSVDTPEDLKKIESRFV"
     gene            10611..11627
                     /locus_tag="PK281_02355"
     CDS             10611..11627
                     /locus_tag="PK281_02355"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48212.1"
                     /translation="MKKWLFSLSVLGTSAVFAQNPLIQENKTWSCYGDYFFLNVKYQL
                     GGDTIFNGKTFRKVLAHGSSVPFVFNADSADYKSAIREENGKVWVIEKGYITEQLLYD
                     FNKNTGDTIRFYRPTGNINQGVLPHYVIGKIYKTNTVTINGIVRKRLYIHDPNMVNML
                     PAQALSQLDPQADVWIEGIGAKTGLFSRMPEWGVVGPQPYLLACVEQNGQSLYAFNSG
                     YNASPEDPCFIIPPDGTGSGSTGGGGSGGTDTLILGIAADQQLKAKLFPNPAKEWVMI
                     SQLSNSRIEIGLYKSDGAQLFFQEAMPENQAYKLSIQNLPAGLYRLRLVQDGKQSWLR
                     LIKE"
     gene            complement(11633..12004)
                     /locus_tag="PK281_02360"
     CDS             complement(11633..12004)
                     /locus_tag="PK281_02360"
                     /inference="COORDINATES: protein motif:HMM:NF020251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOY48213.1"
                     /translation="MKITDRIREIRTIKNISQEYLASFLGIDTSSYHRIERGVCPLSV
                     QRLERIAEALQVSIADLILMEKSGLPVDDPQYAYIKHLEEEIRFLRIQLQDKISPSTL
                     SVNTLNRTSPALNELQRMSRG"
     gene            12307..12726
                     /locus_tag="PK281_02365"
     CDS             12307..12726
                     /locus_tag="PK281_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48214.1"
                     /translation="MASTLGFSNVGNRNKIPLPIKTTIKITGEIGKYDFDCKKISLQC
                     LDFEFGIEIVGERTSATSGSIIATLVMEKTGELQFTFSLPNEPFEEFFNVNKNTTLPQ
                     NLCRVFGFSSMIIPKGEYPNKKNSDGTITATFKVLSK"
     gene            12784..13389
                     /locus_tag="PK281_02370"
     CDS             12784..13389
                     /locus_tag="PK281_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48215.1"
                     /translation="MRNLIFLSVFSIFLSLTSCKRLIYLYHGVHSPRLETPITIEKFC
                     EKKNLEIGELLMMSYDSMITCYRFDLNQTYIFDSSGYRINFNSSFNNPSCGGNVIKVI
                     EGLSRTTYYPRDSIYTLDKEKVKWVYLSNEKPFQKNLNGTNNYTIVCYWNMFTGAPNH
                     RKRIAELKKIISNNKDISVQLIFVNQDFRDGYNIILDAQID"
     gene            13398..13928
                     /locus_tag="PK281_02375"
     CDS             13398..13928
                     /locus_tag="PK281_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48216.1"
                     /translation="MKLIRLFIFLFPLNIFCQHQVCEAFKTRNLISNDNINNSNSISS
                     LSIRPEYQLISIQESYCQLKLFNPQGFQVFYEELNQFPSDEILLQLFEYKLNSNYLIL
                     DSALNKTLEMIRWEIPNNSIESLVSKPCWTLVCYWSTRSALEAQKERMRVWTRFQKKH
                     PEYPLEIYFIQQDISP"
     gene            13979..14905
                     /locus_tag="PK281_02380"
     CDS             13979..14905
                     /locus_tag="PK281_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48217.1"
                     /translation="MKIPILIITSLLLQQVLAQDDPCKFNCFHPTINDTNYKPIPSKY
                     KHALNCSWQFAGLNINSNPNSKSQLKLSGYSLISYQFNHQSKAWKKQLELYDESGFLW
                     ITDSLFIPEKDLTRIKSAISRTSDKPIKLQITSSFQTQKLKGFTPITDSLGIKAPRQS
                     SGFQSPGSFLLNGGLEFQPGKIGSIELSLASGKVMWMLNKQLYSFQNTTELYGISIDK
                     QYKIEGGLNLQSKLEKAFSSRLRWEHTSTWFYPVSKGGDADIQIRNAVFWKPSTTFQA
                     CLRTTYSYNETRFPPGTWTGELSLGYVFVKNP"
     gene            complement(14902..16092)
                     /locus_tag="PK281_02385"
     CDS             complement(14902..16092)
                     /locus_tag="PK281_02385"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOY48218.1"
                     /translation="MLSIETADFSGKKVLIRVDFNVPLDANLHVSDDTRIRAAIPTIQ
                     KVLAGGGAVILMSHLGRPKSGPEDKFSLKHICGKVSELVGKPVAFAADCIGPVAETAV
                     GQLKMGEILLLENLRFYKEEEAGDVAFAQKLAALGDMYMNDAFGTAHRAHASTAVIAQ
                     FFSVQKLFGYVMQGEIDAIDRVLQHPVHPVLAIMGGAKVSSKISIIENVLPKVNHLII
                     GGGMTFTFIKAMGGQIGSSLVEDEQLNLALELLEKAKAMGVQIHLPVDAVIADKFAAD
                     ANTQTTDVNVIPAGWMGLDIGPKTIAQFSEVIRSCKTILWNGPMGVFEMPAFEKGTRE
                     VAMAIVAATEAGSFSLVGGGDSVAAVNQFGIAERVSYVSTGGGALLEYMEGIELPGVA
                     AIRG"
     gene            16186..17337
                     /locus_tag="PK281_02390"
     CDS             16186..17337
                     /locus_tag="PK281_02390"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit"
                     /protein_id="HOY48219.1"
                     /translation="MQFAEIPGHDNLKQRLLHSVHENRIPHAQLFAGPEGSAALPLAI
                     AFAQFVACENRSENDSCGHCGSCVKFRKLAHPDLHFSVPVNWEKPTTELHTQPFLELW
                     RAELLKDPYLGIDQWVDAIDIGNKQPFISNYEANEIIKRLTFKAFESEYKFMLIWLPE
                     KMRVEGANRILKTLEEPPEKTILFLISQHPEELLTTIISRTQLFKVPAYTASEAATIL
                     HQRSGLPLDRCLMASEVAEGNLAQARWMLENSDEADQQLELFRNWMLFCYGFQVGDLL
                     RITDQFAKEGREWQKGFFAYALYMIRQTLLMNHQPQLNRLTEGERTFLTKFSRFFHPG
                     NYAVISNHINEASAHIERNGHAKIVFFDTSLLISDVFRKEKSRVSITRL"
     gene            17455..18873
                     /gene="ricT"
                     /locus_tag="PK281_02395"
     CDS             17455..18873
                     /gene="ricT"
                     /locus_tag="PK281_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013550307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HOY48220.1"
                     /translation="MGCTSCSSGRGGLPAGCKNNGACGAGGCDKLPVFDWLANMELPD
                     GKAPYDIVEVRFKNSRKEYFRIGEVSGLVVGDAIAVEASPGHDIGMVSMTGELVRLQL
                     KKLGIPADGLFKAVYRKARPVDMEKWEASVEKEQTTMMRARAIAVELKLNMKISDVEY
                     QGDGTKAIFYYTAEDRVDFRELIRHLAEEFKIRIEMRQIGSRQESSRLGGIGSCGREL
                     CCSTWLRDFRSVNTSAARYQQLSLNPQKLAGQCGKLKCCLNYELDSYLDAIKDFPEGT
                     PRLETKKAAAFHQKTDIFKRVMFYAYVDDPGNFIAVPISRVKHIMELNKNGEKPDVLL
                     SEKEIPKVVKELDYDNVVGQDSLTRFDSEKRKKKKKKKKPGSGINSGSEATPVSAAAP
                     QTSTPAKVQRPIQPNRPQVQRPPQANQANQQNRPQVQRPPQTNPPNQQNRPPQQRPNN
                     PNNNKRPNHGPRPPKPPQTPEA"
     gene            18887..19333
                     /locus_tag="PK281_02400"
     CDS             18887..19333
                     /locus_tag="PK281_02400"
                     /inference="COORDINATES: protein motif:HMM:TIGR03511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldH"
                     /protein_id="HOY48221.1"
                     /translation="MLLVLFSSSCDKQRVFEENTPIPDNIWKADQPVRFEVTIDDTLS
                     GHNFLMNIRHAEGYPYSNLFVFLKTDFPNGKAARDTIEFILQNPDGKWSGSGLGDLWD
                     SQIQFKQNIRFPLKGKYTFTIEQAMRAPALPMIMEMGLRIEKYSAE"
     gene            complement(19549..20262)
                     /locus_tag="PK281_02405"
     CDS             complement(19549..20262)
                     /locus_tag="PK281_02405"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOY48222.1"
                     /translation="MKSLFLSLCCAVILLVSSCTPEPQACITVDRTHVQQNESLAFTS
                     CALDAKRVVWNFGDGTAEVEGEQASHTYAQPGVYLVEMKALSKKDKKWDRTSVIINVD
                     PLKTRYLTRLQVNSFNINNISGTTWDVAPGNWPDVFFEYAVAGSALRSTINPPINELQ
                     LNQCPVFWDFSSSVSKPILANNDYIIQMLDNDGTLLQPSSELMATFNLNPATATPSAP
                     GVITLTNSNFQIELHFIEL"
     gene            complement(20377..20979)
                     /locus_tag="PK281_02410"
     CDS             complement(20377..20979)
                     /locus_tag="PK281_02410"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008990208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HOY48223.1"
                     /translation="MAFELPKLSYEYAALEPNIDARTMEIHHSKHHQAYVNNLNAALT
                     GTAEEGKSLEQLMSNISKLSPAVRNNGGGHYNHSLFWEIMGPNAGGAPTGELADAINQ
                     AFGSFDKFKEDFAKAGATRFGSGWAWLVKHDGKLTVTSTPNQDNPLMDLADVKGFPIL
                     ALDVWEHAYYLHYQNRRPDYISAWWNTVNWNVVADRFKKG"
     gene            complement(21141..>22143)
                     /locus_tag="PK281_02415"
     CDS             complement(21141..>22143)
                     /locus_tag="PK281_02415"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOY48224.1"
                     /translation="AQIAMELRTALFGKEISKFRDADDDAEITLRLASSYRDKVDDLL
                     NMQIAFLDMSTGMFKQVPVSAVASVKYEKAFSSINRKNQRRVLTLSSNVLTGYTANEI
                     VRSIDQIIPSLDVPEGYEVKMTGEQEQQKETSDFLSLAFLGALALMFLILVTQFNSLV
                     KPFIIFSTVVFSLIGVFFGYALTGMTFSIVMTGVGIFALAGIVIRNGILLIEFIDELR
                     ARGLSPEEAIAEGGSTRMTPVILTASAAILGLIPLAIGVNIDFNGLFAHLEPHFFLGG
                     DNVAFWGPLAWTMIYGLIIATFLTLMIVPAMYLMYLKVKVRFGMEKKGEKEHDGTML"
BASE COUNT         6552 a         4875 c         4461 g         6255 t
ORIGIN      
        1 aataagcttc tagattttaa cttttaatca ttctcggaac aagcaatctc agtaatttct
       61 gattgaattc cggaatttcc ttaccgagga tggcaaaaaa ctcatcgtcg aaagcttcca
      121 ctattttgac tgtttgaagc actttggttt ttcctgcagg tgtgagtgca actgatttag
      181 cgcgcgtgtc ggtactgtgt tctttgcgct caattaagtc cttacgttgt aaagtacgta
      241 aaacggtaga ggttgtcatc gggtcgattt tggtgtgctg cgacaatatg atttgagtga
      301 cttccttttt ttcgagtgtt aaccaatgca tactcgccag cagaacgaat tgcgaatgcg
      361 ttaaatcgaa aggttcgagc accttacgga tttcgcgctg ccaaagactg gttacctgcc
      421 agagtaaaaa tccggagctg tcgttggctt cttcaaactg aaaggggttt gtttcagaac
      481 tcattgggaa gcatgtgtga agcaattaag cgaaagtctt tttcaccgat ttcaaagaaa
      541 ccaaaacgaa acggatatcc ccaatgcttt ttgtcgggaa taaattctaa gtgtggaatg
      601 agcgatgaaa tggccgtttc ctcgcatgga taaaatgaaa catttctacg aaaaggcaca
      661 aactcgggcg acatttcaaa ggcataaaca tctgcgtcgc aagcttgtcc gattgctgta
      721 aatgcctgac atttttcctc gctctgcatg gagattttgg aggagtagaa aataatccaa
      781 tcgccttttt gcatgcgttt aagaggtgct tgtttaccat ggcacacttg catgaaaccg
      841 cccgattcgc ctcgtttggt atgatcgcgg gagactgctc cgatgaagaa tttttgtgct
      901 ctcattgcat gtgggcttta tcgatgaggt tttgcgtttg ctgctcagct cctttaatct
      961 ggttttgagc gatgattcgg ctccataaaa agctcaatag gccgcgtact tccacttcta
     1021 tattgatttc tatgccctgt tctgtttggg taaagcggtg tgtggttcgc attttagcca
     1081 agggcagata tgccacatca ctaaacacat tgggagcagt gtattccaaa acgcacagct
     1141 tggttttagg tccatctttg ggctttaggt agaattcagc gccctgtttt actggacctt
     1201 ttatgctcgt ccatgaaata tcgtgatccc aagtattcca gttttgtata tcggttaatg
     1261 tgttccaaag ctgattgata gtcgcctttt gggtgatgat ggttgcgttt cctttccaca
     1321 tggttttgag tatgagtcga caaatatact aagtgcgcat ataatatgca aacatatcga
     1381 gtgtcgtctt atttttttct tcacatgcag gtggatgaat cgctgtgaga ctcttgctat
     1441 gaacgctctt tttctacctt tatcgcccca aaaccatcca aactatgcct tcttccacgg
     1501 attacatcct ggtaatcggt tcttccggac aaatcggtac agaactcgta gcccgacttc
     1561 gtgaaatcca cggttcgtct caagtaatag cagccgattt gaacattccc gccgaatcgg
     1621 acggtccatt tgagcaactc aatgttttag atacacagcg cttgtcggag atacttgcta
     1681 agtatcgcat atcggaagtt tatctgcttg ctgcgatgtt atctgcaacc ggagagaaat
     1741 tcccgatgaa agcttgggat ctcaacatga acggattatt tcatttgttg gatgcagcaa
     1801 aagaaggcaa gattaagaaa ctctactggc caagttccat cgctgttttt ggaccaacta
     1861 cacctcgaat tgaaacgcca caaaacacta ttgctgagcc aaccacggtt tacgggattt
     1921 cgaaattggc aggagaacgc tggtgcgaat attaccatca gaaatttggg gtggatgttc
     1981 gatcgctgcg ttatccggga ttaattggct ggaaatcggc acctggcgga ggaacgaccg
     2041 attatgctgt gcacattttt catgaagcga ttgagaaagg ttcgtacacc agtttccttt
     2101 cggctaatac gcgtttgccg atgatgtaca tgcctgatgg tattaacgcc accatccaaa
     2161 ttatgcaagc tcccgccgag cagattcgaa ttcgttcgtc atacaatcta gcagcaatga
     2221 gcttcacgcc tgataatctg gcggcagaaa tacgcaaaca catccctaat ttcagaatcg
     2281 attacgcacc cgattttcgt caagccatcg ccgattcctg gcctgcttcg atcaacgata
     2341 gcgacgcccg taaagattgg aactggaatc ccgaattcga tttagctaag atgaccgaag
     2401 atatgttggt gaatttgcgc gcgatgaaaa cttcggttta gattacttct cctgccccaa
     2461 agagaattcc cgtgcgctga gctcgtcgaa acgctatcca atttcaaaat aaattcatcc
     2521 gaattcgtct gcatttgcgg gaaccgaaaa gcatgggtat tttagccctt tctattctta
     2581 acccatgaac acgcaggatc tttttattta caacacactc agccgccaaa aagaacggtt
     2641 cgaacccatc aatgcacctt ttgttggttt gtacgtgtgt ggtcctaccg tatatgggga
     2701 agcgcatttg ggacatgctc gtccctatgt gacttttgat gtgcttggtc gctacttgag
     2761 ctccattggc tataaagtcc gctatgtgcg caacattacc gatgtaggac atttggaaaa
     2821 tgatgcggat gaaggggaag ataaaatcgc gaaaaaggca cgtcttgaga agctagagcc
     2881 tatggagatt gtggaactat acaccaatag ttttcacgaa tcgatgcgga tgttgaataa
     2941 caatcaccct agcattgaac cacgtgcatc tggccacatt ccagagcaaa tcgaaatgat
     3001 tcagaaaatc atcgaaaacg gttttgctta tgagtcgaat ggttcggtgt attttgatgt
     3061 cataaaatat agtaaaacca acgattatgg gaaactgagt ggccgtgtac tagaagattt
     3121 aattgccggt gccggaaatg atcgtcgggt attggaaggt caagaagaaa agcacaatcc
     3181 agaagatttt gccctttgga aaaaagcgga tgcttatcac attatgcgtt ggagttcacc
     3241 ttggagtgat ggttttccgg gatggcatat tgagtgctca gcgatgagtc gcaagtattt
     3301 gggtgaaacg ttcgatatac acggtggtgg aatggatctc gtttttccgc atcacgaaag
     3361 tgaaattgct caatccaaag gcagttgcgg tcatgctcct gtgaagtatt ggatgcacaa
     3421 taacatgatc accatcaacg gacggaaaat gggtaaatcc ttggggaatt ttatcacctt
     3481 gaaggaattc ttcactggct caagtgcttt gcttgatcag gcatacagcc caatgaccat
     3541 tcgtttcttt atccttcagg cgcactatcg tagcacgctc gatttttcga atgatgcact
     3601 caaggcagct gaaaaggcct acaaacgtat catcaacggt ttacgtagct tgcgtcaatt
     3661 ggactattcc agtgatggcg gaacaataga tgctgatcta aaaattcgca tcgaagctgc
     3721 tgttgttcag gcctatgccg gcatgaatga cgatatgaat accgctgttg ctttggcttc
     3781 cttgttcgat ttgttgaaag tgattaattc gttaaaaggc ggacaaatag ctatgtcggc
     3841 actttcgaaa gacacgttcg acttgatgaa gtcgacctat atccattttg tagagaacat
     3901 atttggctta cttgaggaga aagggaagtc ggacgatact ttgggtactt tgatgaacgt
     3961 tatcttggga gattatgtag atgcaaagtc gcgtaaagat tatgcaacag tagatcgcat
     4021 tcgtgcacag gtaaaactag aaggattagt aattaaagat tcaaaaacag gaattagctg
     4081 ggattatgaa gaataaatgg ctgggagcga gtctcctatt aatggcagga atatggactt
     4141 cttgcggtgg accatcatcc agtggcgatc aaccagttcc tactgaaact cccaagcagg
     4201 ctaaattagt tccggctgat cttccagcat tcaatgccga tacagcttat gcgattactg
     4261 caaagcaagt agcgtttggt ccacgagttc caaataccga agcgcatgta aaatgtgccg
     4321 attacctgat tgctacactt aagcgttatg gtgcagaagt ggtagtgcaa agcggcaaag
     4381 tgacagccta cacaggtgaa tctttaagca tgcgaaatat cttagctcga gtgaatccaa
     4441 gtacgtcaac gcgaattatg ctttgtgctc attgggatac tcgaccattc tcggatcagg
     4501 atattatgaa accgaaagca cgtttcgatg gtgcagttga tggagctgcg agtgttggga
     4561 tattattgga aatagctcgt gtattacaag ctcatcctgc tccaattggg gttgatattg
     4621 ttttgtttga tgccgaagat tatgggaatc cgaaggaaag caatacatac tgtttaggtt
     4681 ctcagtattt tgcaaccaat ccaccaatcc cgaattttag tccgaaatat ggcattctac
     4741 tcgacatggt tggtgcaagc ggagctacct tttaccgtga aggattctcc atgcaatatg
     4801 cacctgatgt catgaatcgt gtgtggagta taggagcaga attaggctac agttcgtatt
     4861 tcagaaacga tccatgtggt tctgtaacag atgatcatta ttttgtcaat acgattttag
     4921 gaataccgac aatcgatatc attcatcacg atcctcaaaa aggatttggg gattactggc
     4981 atactcagca agataatatg aatgcagtcg acaaaaccac tttaggagtg gtgggaaaaa
     5041 ccttgctagg tgttgtttat agtgagaaat cgggaatttg atttattctt ttaaggcata
     5101 aaaaaacgcc atcttctttc ggagatggcg tttttgtttg gatgaaatcc gatcaaggga
     5161 aaactcccaa tgtattgtat gactgaacaa tcttcttaat cgcaaccacg aaagcagctg
     5221 tacgaagatc aggaatgcca gatccacttt ttaactcatc acggatagcg ttgtatgaat
     5281 tgatcatggt ttcttccaaa ccggagtaaa ctaaatctaa ctcatcagca ccacgctcga
     5341 ttagtttttt ctgagcatca gtagcgatac gaccagtcat ttcttctaat acagaaatag
     5401 tattatgacc agcagcttcg tcccaacgtt ttcctaaacg accaaaacgt acgtgactta
     5461 ggtttttcaa ccattcgaaa tacgaaacgg taacaccacc agcgttgagg tagatatcag
     5521 gaatgatgta aacacctttt gcatttaaaa ttgcttctgc atcaggagtt acaggaccgt
     5581 ttgctgcttc accaatgatt ttacatttga tgcgatcagc gttagtacca ttaatttggt
     5641 tctcaagagc tgcaggaatt aatacatcac actcaatttc aagagcagcg tatttatcta
     5701 cattcgttgt tcctgggaaa ttaaggatac ttccagtttt gttgcgatgt gcttctaatg
     5761 catgaggatc gattccatca gcactgtaaa tagcaccatc ccactcagcg atagaaataa
     5821 tctttccacc accttcgtga acgaatttag cagaatggaa acctacgtta ccaaagcctt
     5881 gaacaacaaa cgtttttcct gccatacctg gagtaagtcc ccaagctttg caatcttcca
     5941 cattgcttaa tgcttcgcga ataccaaagt aaacacctaa tccggttgct tctttacgac
     6001 cacgaatacc accttgacta actggttttc cagttacaca agccattgca ttcaactcat
     6061 ttgggaagaa tgtagcatat gtatcaacaa tccaagccat ttcgcgttca cccgaaccgt
     6121 aatccggagc aggaacgtcg ataccaggac cgatgaagtt tttcttaatc agctcagacg
     6181 tgtaacggcg tgtaatacgc tccatttcat cggctgataa tttgcgcggg ctgattttaa
     6241 taccgccttt tgctcctcca aacggaacat ctacaatggc acatttgtaa gtcataagag
     6301 cggccaaagc catcacctca tcctgattta ctgctgtgct gaaacgaata ccgcccttag
     6361 taggtagttt gtggtgcgag tgctccacgc gatatgcttc aataacctga tatccatcgc
     6421 ccattttcac agggaacttc atccggtaaa cactgttgca atatttgatt tgatctagaa
     6481 gaccagctga tacatcgacg aatttcgccg cgcgatcaaa gtacttgagg acatcctgaa
     6541 agaattcatc ctctcttttt tccgtaatgt cggacataaa cggtttatta aagattgata
     6601 attggggttg actgagccaa acgtacacaa atttttacgg ttggatgatt ttgatataag
     6661 gaaatatgtc gcacagaatc agcttgttgg gcattttttt cgacgaggct gtacttttta
     6721 cccttctgaa aaaggcgaga aaacgcaact ctgggttggg aattcagacc gatttatccc
     6781 tgaggagcaa aaagagcttt cagttcggtt gcttcatccg gcttcatttt gcctgctaaa
     6841 atcaaactta actgtcggcg acggagtgca ccttcatagc gacttttttg taattcggtt
     6901 tcaggaacca attcgggaac tgctatcgga ttgcctaatt gatctaccgc tacaaacgtg
     6961 taaatggcct cgttgtgacg cactcgttct ccagttgaat gattctccac ccaaacatca
     7021 ataaacactt ccatcgacga ggaaaaggca cgagaaacat gcgcttccag cgttacaata
     7081 tctcccaaac gaatcgggcg atcaaacgat acattattca ccgaagctgt tacgacaata
     7141 cgtttacaat gacgatgtgc tgaaatggct gcaataatat ccatccaatg caggagtttt
     7201 cctcccatta gatttccaag ggtattggta tcattgggaa gtacgatttc agatgaaacg
     7261 gcaatggatt cgtgggcaaa cttttttgtt ttcataagcg gttgcaaagg taacatgcat
     7321 tgctgcttta cgaaacaaat caagtgggat taagttcgga tacatccaag ctccacctca
     7381 aaccacttat atacatgaat taggacaggt taaatttcag cctgctatat ttgcagcatg
     7441 tacctatacc taaaagccat tcatattatt ttcatcgtta cctggtttgc cggacttttc
     7501 tacatgcctc gcttactcat ttatgatgta gaagcacgtg aaaaagccga gccgggcaga
     7561 agcattctag tagaacaatt ccgaatcatg caaaaacgct tgtggtttgg aattacttgg
     7621 ccttcgtttg ttttaacgat gatacttggt ccttggttgg tgattgaaac aggatatcct
     7681 tggacaacga gttggttggt tgttaaactt ggattggttg ccggattagt tggataccac
     7741 tttagtttac aggtaatctt tcagcaatta gggaaagatg tggtgaaata tacgtccaat
     7801 caactccgac tttggaacga agtagccacc ttgtttttgg tatccatcgt attcctcgtg
     7861 gtgattaaaa acgagttgga catgctctat ggattagccg gtttaattgg gctgattgcc
     7921 attctcatgt tagccatccg aatttacaag cgtattcgga acgcaggtta attatggttg
     7981 agcaacagat ttaagacgtt ccatgttttg gagcgccatg ccgaaatcag gataaatttt
     8041 caaagcctca ttcaaggcga tttcagcttc tgtatagcgt ttcatatcga ttgctatcgt
     8101 agctcgaatg tttaatgctg cagcttttac aggaacttcc tgcatttgtg ctgtaccatt
     8161 aacttccacc tgaagtccaa caacttcttt atcagaatta ggattggcaa tgatagcatc
     8221 aatattcttc tcacattcac caaaacgctg gagcatatac tgtaaggctg cggttttgta
     8281 tcggaaaata ctcaaattcg atttagcaat aagaaactca taatcgctta aggactctgg
     8341 gtagcgccct agtccttcta aagcctgagc tctaacttcg tgtaaagtca catcatcggg
     8401 ctttttctgc aagatttcac tcgctaattt gtgggattga taatacaatc ctctaccatg
     8461 atacaacaaa caaagtgtgt ctttccagtt tgttaatgct ggattgattg ccaaaatctg
     8521 aaatacagca gaagttgctt gctccaaatc gttcaactcc atcgccgaac gataaacttt
     8581 ctcttgagtt gcaactggtc cagtagatgc cggttttacg ggcggtttag ctggtttgct
     8641 ggtttgagat tgtgccaatg gagaaaatcc caaggcaata gctaagaata gaaaacgaaa
     8701 aatcattagc gaattaataa ccaggcttct tcattgagcc ctttacgaat cgaagttaat
     8761 tcatcagttg cctcctgaat actaggataa ttaccgtaaa caactcgata aagaccagca
     8821 ggtgtgcgat ctagcacata cgaactaaat cctttttcac tgaggtaatc taccattcca
     8881 tttgcattca cttcggtcga ataacatccg gcaacaataa aaatctgcgc actttcggca
     8941 ctgaataatt cagctacagg tgctggaata gattttctag ctgctggcaa tgcttccgga
     9001 ctttgaacgg ataaagcaga tgctgaaata gaagctgact gagagaaaat atttaaatca
     9061 gcagttgaaa tgcctttccc ttcgggcaaa cttgcgttta caccgataaa tgcagcaatc
     9121 aacaatactg gtatcgcttg caatactcga cgaactggga acttacgact aaccttaaca
     9181 ggtggaacca attcacgatt tacgaaaaca ggttctgggc gacgttctgg aactgggaag
     9241 cgattgatag gctgaacctg aatcgcatac aaaccaatcg actcgggtag gtaattgaca
     9301 ctttgagtag ccttaaactg gatgtttcgt tctacatcag tgttgaaagt cccaatttga
     9361 gtccattcaa tacgttctcc cgctaaaaga ttagcattta gcttctcaac gtattcactt
     9421 agatacaaac gcgaaagcga gaaggataag cccgaaacac gtgcatattc attcaccaac
     9481 aatccatcat ctgttttcaa actccggtta aataccaatt gcttggaagg agcttgaaac
     9541 acatgtgtta ccggatgtac gcgcgcagga gcataattac ctatcaaacc tccaaaaccg
     9601 ggaaggatga tacaatcatg cacatgtagc aattcagcca caaggctgga aacctgtaca
     9661 ttacgtttgg agaactcgct gtgatccatt tgctcaaata tagcgtggtt tttcttcaga
     9721 caaagcgact ttctattttt ttcaaatctt cgggtgtatc tacactccaa ctttcgtgtt
     9781 cactcaaggc tgtcatgatg cgataaccat tctcaatcca gcgtaattgc tctagttttt
     9841 cggttaactc aagcgatgat ggagctaacg aagcaacgtg taataaagtt gcagcacgat
     9901 atccatataa gccaatatgc tgaaaatggg cggtcaaatc gggcagcttt ccttgtggtg
     9961 catctcgttc aaatggaatg ggtaagcggc tgaaataaat tccataccca tgaatgtctc
    10021 taacaacctt aatcgtattg ggattcagca catcttcgcc atacgaaaaa cgtctaacta
    10081 aagtgcctaa ttctacctgt tcattcgaaa agcagccaca caacacatca atctgaccgg
    10141 gatcaataaa aggctcatct ccttgaatgt tgatgattac agcatttgaa tgaactgctg
    10201 atcctaatat ccgaaccact tcggcacatc gatctgttcc acttgggtgt tcaggagaag
    10261 tcatcactac ttcaccccca aaatagcgca cttctgcttc aattcgttca tcgtcggtag
    10321 caacaatcac ccgactcaac attttgcttt gaagcgcttg ttctaccacg cgacgaatca
    10381 tcgtttttcc ggagatttct accaatggct ttcctggaaa acggctgctc gcgtaacggg
    10441 caggaatgat tccaataaac tgaactgttt gcatgctcca aagatacagc tctgcttcga
    10501 aaacactttc tctttccaaa acttacatgc ctgtatcttt tccaatcacc gcaaggtata
    10561 atctcacatg aaacagcctt cgactttact catgcttaat cgttcaggtg atgaaaaagt
    10621 ggctgttttc cctatccgtt cttggtacaa gtgccgtttt tgcacaaaac ccattgatcc
    10681 aagaaaataa aacttggtcg tgctacggcg actatttttt cctcaacgta aaataccaac
    10741 ttggcggtga taccatcttc aatggtaaaa cattccgaaa agtgctagca catggcagct
    10801 ccgttccctt tgttttcaac gccgatagtg ccgactacaa atccgctatc cgcgaggaaa
    10861 atggaaaagt ttgggtgata gaaaaaggat atatcaccga gcaattactc tacgatttta
    10921 ataaaaacac aggtgacacc attcgctttt atcgtccaac ggggaatatc aatcaaggtg
    10981 ttcttcccca ttatgtgatt ggtaagatat acaaaaccaa cacggttacc atcaacggaa
    11041 tcgttcgcaa acgcttgtat attcacgatc ctaacatggt gaacatgctt ccggcacagg
    11101 cacttagtca actcgatcct caagctgatg tgtggattga aggcattggc gccaaaaccg
    11161 gattattctc gcgcatgccc gaatggggag ttgttggacc gcaaccctat ctacttgcct
    11221 gtgtagaaca aaacggacaa agtttatacg ccttcaatag cggttacaat gcttcacccg
    11281 aagatccttg ttttattatt cctcctgatg gaacgggtag tggaagcact ggcggcggcg
    11341 gctcgggtgg aaccgacacg ctcattttag gcatcgctgc tgatcagcaa ctcaaagcca
    11401 agctatttcc taacccagct aaggagtggg tgatgattag tcaacttagc aatagccgca
    11461 tcgaaattgg cttgtacaaa tcggatggag cccagttgtt cttccaagaa gccatgcccg
    11521 aaaatcaagc atataagctg tctatccaaa atttacctgc cggattgtat cgactacgtc
    11581 tggtgcaaga tggtaaacaa agttggctcc gattgattaa ggaataaatc gttcatcctc
    11641 gcgacatgcg ttgaagttca tttaatgcgg gtgaagttcg atttaacgtg ttcacggata
    11701 gagtagaagg actgattttg tcttgcagtt gaatgcgtag aaatcgaatt tcttcttcga
    11761 gatgttttat ataggcatat tgcggatcat ccacaggcaa acctgatttt tccattagaa
    11821 taagatcggc aatactaact tgtaatgctt cagcaatacg ttcaagacgt tgcaccgaaa
    11881 gcggacaaac tccacgttcg attcggtggt aactggatgt gtcgattcct aaaaaagaag
    11941 ctaagtattc ttgagaaatg tttttaatag tgcggatttc tctaattcta tctgtgattt
    12001 tcataagaca tagtataagg attaatagtg tagccaatat acaagtaaaa tagtttaagt
    12061 ctgtttgcat cagacgaaaa agttttgcat gtggcgcaaa acaacatata gcaataccgt
    12121 tttatgtttg tgaaacaatt cgaaaagcca tggtctgaat taaaattgga gaagaaaatc
    12181 cgggaggatt atccaatccg gcagtcgcgt tcgcttcacc ataattggaa acgtaaagtg
    12241 attatttaaa ccttaattaa ttaaattatg aaaacgatta aattattttt tatgatgtta
    12301 tgtgttatgg catcaacact gggtttttca aatgtgggaa atcggaataa aattccactt
    12361 ccaataaaaa caacaattaa aattacaggt gaaattggga aatatgattt cgattgcaag
    12421 aaaatatctc tacagtgcct tgactttgaa ttcggtatag aaatagttgg agaaagaact
    12481 tctgctactt caggatccat aattgcaaca ttggtcatgg agaaaacagg agaattgcaa
    12541 tttactttta gtcttccaaa tgagccattc gaagaatttt tcaatgtcaa taaaaacaca
    12601 actttaccgc aaaacttgtg tcgggttttt ggtttttcgt ctatgattat tcctaagggc
    12661 gaatatccca acaagaagaa ctctgatgga acaataacag ctacattcaa agtgctttca
    12721 aaataataat aatgtgtcac aatggttaga attagcattg tgacacattt aaattcttgt
    12781 tgtatgagga atcttatctt tttatcagta tttagcattt ttcttagttt aacctcttgc
    12841 aagcgcttga tatatttata tcatggagtg cattcaccaa gattggaaac accgataaca
    12901 attgagaaat tctgcgagaa gaagaacctt gaaataggtg aattactaat gatgagctac
    12961 gatagcatga ttacttgtta tcgttttgat ttaaatcaaa cttatatttt tgattcaagt
    13021 ggatatagaa ttaatttcaa ttcatcattt aataatcctt catgtggagg aaatgttatc
    13081 aaagttattg aaggattgtc acgaactacc tattatccaa gagatagtat atacacgcta
    13141 gataaagaaa aggtcaagtg ggtttattta tctaatgaaa aaccgttcca aaagaattta
    13201 aatggcacta acaattacac tattgtttgc tattggaata tgtttacagg agctccaaac
    13261 catagaaaac gtattgcaga attaaaaaaa ataatttcaa acaataaaga tatttctgtt
    13321 caattaatat ttgtaaatca agattttagg gatggttaca atattattct tgacgcccaa
    13381 atagattaat ttataatatg aaattaataa gattatttat ttttttattc ccattaaata
    13441 tattctgcca acatcaagtt tgtgaggcat ttaaaacaag aaatttgatt agtaatgaca
    13501 atattaacaa ctctaattca atttcctccc tctccattcg tcccgaatac caattaatct
    13561 ccatccaaga atcctattgc caactaaaac tcttcaatcc gcaaggtttt caagtcttct
    13621 acgaagaatt aaaccaattc cccagtgacg aaattctcct gcaattattt gaatataaat
    13681 taaattccaa ttatttaatt ctagatagtg cattaaataa aacactcgaa atgattcgtt
    13741 gggaaatacc caacaattcc atcgaatctc tagtatcaaa accgtgctgg acgctcgtct
    13801 gttattggtc tactaggtct gcacttgaag cccaaaaaga gcgaatgcgt gtttggactc
    13861 gctttcaaaa aaaacatccc gaatatccgc tagaaatcta ttttatccag caggatattt
    13921 caccttaaat cgttccggtt gatcaggcgt tccgaattca cctcgccatt cttcaaccat
    13981 gaaaatacct atactgatta tcacttctct tcttttgcaa caagtgcttg ctcaagacga
    14041 tccctgtaaa tttaactgct ttcatccaac aataaatgat actaactaca aaccaatacc
    14101 atccaagtat aaacatgcac tcaactgctc ctggcaattt gctggactga acataaattc
    14161 taatcctaac tcaaaatctc aactcaaact atccggttat tctctcatta gttaccaatt
    14221 taatcatcaa tcaaaagctt ggaaaaaaca actcgagcta tacgatgaat ctggtttctt
    14281 gtggattacc gacagtctat ttattcccga aaaagacctc acacgaatta aatcagctat
    14341 ttctcgcaca tccgataagc caattaagct acagataaca agctccttcc aaactcaaaa
    14401 actaaaaggc tttactccaa ttaccgattc tttgggaatc aaagctccaa ggcaaagttc
    14461 cggatttcaa tctccaggct cttttctgct aaatggtgga ttagaatttc aaccagggaa
    14521 aatcggctcg attgaactca gtcttgcttc tgggaaagtc atgtggatgc tcaataaaca
    14581 actctacagc tttcaaaata ccaccgaact atatggaata tcaatcgata agcaatacaa
    14641 aatcgagggc ggactgaatc tccaatctaa actcgaaaaa gcctttagtt cacgattacg
    14701 ttgggagcat actagcacgt ggttttatcc tgtgagtaaa ggtggagatg ccgatatcca
    14761 aatccggaat gccgtttttt ggaaacccag cacaacattc caagcctgtt taagaaccac
    14821 ctattcgtat aacgaaacac gctttcctcc gggcacttgg accggtgaac tctcacttgg
    14881 atacgtcttt gtcaaaaatc cttaaccacg aattgcagca acacctggca attcaattcc
    14941 ttccatgtat tcaagcaaag ctccaccacc tgtgctcacg taggaaacac gttcagcaat
    15001 gccaaattga ttcaccgcgg ctaccgaatc tccacctcca acaagagaga aacttcctgc
    15061 ttctgtggca gctacaatag ccatagctac ttcgcgtgtt cctttttcaa atgcaggcat
    15121 ttcaaaaact cccataggcc cattccacaa aatggtcttg catgagcgaa tcacttcgct
    15181 aaactgagca atcgttttag gtccaatgtc taatcccatc caaccagctg gaatcacatt
    15241 cacatcggtt gtttgggtat tagcatcggc ggcaaactta tctgcaatca cagcatcaac
    15301 aggtagatga atttgaacac ccatcgcttt tgctttttcc aataattcca atgcaagatt
    15361 caactgttca tcttctacga gagaagaacc aatttgtccg cccattgctt tgataaacgt
    15421 aaacgtcatt ccaccaccaa taatcaaatg attcacctta ggaagaacat tctcaataat
    15481 cgaaatcttg ctgctcactt tagcaccacc cataatcgct aacaccggat gaaccggatg
    15541 ctgcaacaca cgatcaattg catcaatctc tccttgcatc acataaccaa acagtttttg
    15601 taccgaaaaa aattgagcta tcaccgcagt tgaagcatgg gcacgatgag ccgttccaaa
    15661 tgcatcattc atgtacatat cgcccaatgc agctagtttc tgtgcaaatg ctacatcacc
    15721 agcttcttct tccttgtaaa aacgaagatt ctccaacagt aaaatctcac ccattttcaa
    15781 ttgtccaaca gcagtttcgg ctaccggacc aatacaatca gctgcaaaag caacaggctt
    15841 tccaactaat tccgacactt ttccgcaaat atgcttcaac gaaaatttat cctccggtcc
    15901 cgacttagga cgacccaaat gcgacatcaa aatcactgca ccgccaccag caagtacttt
    15961 ctgtatagta ggaatcgcag cacgaatgcg ggtatcgtca cttacatgca gattggcatc
    16021 caaaggcacg ttaaaatcaa cacggataag aacttttttt ccagagaaat cggcagtttc
    16081 gatcgagagc ataggttgaa ttttgtgctg gcgaatttga gaaaaaatgc gctagcctcg
    16141 tactagaaat attccttccc aagccttttt ccgtactttc gccccatgca gtttgccgag
    16201 atacccggtc atgataacct taagcaacgc ctcttacaca gcgttcacga gaatcgtatt
    16261 cctcatgctc agctatttgc cgggcctgaa ggttctgcag cattgccttt agccattgct
    16321 tttgctcagt ttgtagcttg cgaaaaccgc tccgagaacg atagttgcgg tcattgtggt
    16381 tcctgtgtta agtttcgcaa actagcacat cccgatttgc atttttcggt tcccgtaaac
    16441 tgggaaaaac ccaccaccga actacatacg caaccctttc tcgaactctg gcgcgccgaa
    16501 ttgctcaaag atccatacct cggtattgat caatgggttg atgccatcga cattggaaac
    16561 aaacagccct ttatttcgaa ttacgaagcc aacgaaatca tcaagcgttt aacatttaag
    16621 gcattcgaat ccgaatataa attcatgctc atttggttac ccgaaaaaat gcgggtcgaa
    16681 ggtgcgaatc gcatcctcaa aacactcgag gaacctccag aaaaaacaat tctcttcctc
    16741 atctctcaac atcccgaaga attacttaca accatcattt cgcgtacgca attgttcaaa
    16801 gtgccagcct acacggctag cgaggctgca acaattttgc atcaacgttc gggtttacca
    16861 ctcgatcgct gcttgatggc atccgaagtt gcagagggaa atcttgcaca agcacgttgg
    16921 atgctcgaga acagtgatga agccgatcaa caactcgaat tgtttcgaaa ctggatgctt
    16981 ttctgttacg gttttcaagt gggcgatttg ttacgtatca ccgatcagtt cgctaaagaa
    17041 ggtcgcgaat ggcaaaaagg atttttcgcc tacgcacttt acatgattcg tcaaacttta
    17101 ctgatgaatc atcaaccgca actgaatcgt ctaaccgagg gcgaacggac ctttttgacc
    17161 aagttcagcc gcttttttca cccaggtaat tacgctgtca taagcaatca tatcaacgaa
    17221 gccagcgctc acatcgaacg caatggccac gctaaaatcg tcttcttcga cacctcgctg
    17281 ctgatctcgg atgtgtttag aaaagaaaag tcgcgcgtat ctataacccg tctttaatga
    17341 cggactttca ctttttttcg ccagttttgt ggcatcggat ctcgttaggc tacgtgctga
    17401 atttaggatt gcctacaaac gattgatctg ttgatatata agacctgagg tagaatggga
    17461 tgtacaagtt gctcttccgg aagaggcgga ttgcctgccg gatgtaaaaa taacggagct
    17521 tgcggcgcag gtggttgcga taaactaccc gtgttcgact ggctcgccaa tatggaactt
    17581 cccgatggga aagcaccata cgacatagtg gaagttcggt ttaaaaacag ccggaaagaa
    17641 tatttccgaa taggagaagt gagcggttta gttgtaggcg acgctattgc tgtggaagcc
    17701 tcacctggac acgatattgg aatggtttcg atgaccggtg aactcgttcg cttgcaattg
    17761 aagaaactgg gtattccagc cgacggatta ttcaaagccg tttaccgaaa agcccgtccg
    17821 gtggatatgg agaaatggga agcttcggta gaaaaggagc aaaccaccat gatgcgcgct
    17881 cgtgctattg cggtggagct taagctgaac atgaaaatca gcgatgtaga atatcaaggc
    17941 gatggtacta aagccatttt ctactacact gccgaagatc gagttgattt tcgtgaattg
    18001 atacgccact tagctgaaga atttaagatt cgtatcgaga tgcgccaaat tggttcacgc
    18061 caagaaagct ctcgattagg tggaattggc tcatgcggac gagaactctg ttgctctact
    18121 tggttgcgcg atttccgttc ggtaaacacc agcgcggctc gttatcagca attgtcgctc
    18181 aatccacaaa agttagccgg acaatgcggt aaactcaagt gctgcttgaa ttacgaactc
    18241 gattcttacc tcgatgcgat caaggatttc cccgaaggaa ctcctcgtct tgaaactaaa
    18301 aaagctgccg cgtttcacca aaaaaccgac atcttcaaac gcgtgatgtt ctacgcttac
    18361 gtagatgatc cgggaaattt cattgctgtt cctattagtc gcgttaagca tatcatggaa
    18421 ctcaacaaaa acggggaaaa acccgatgtg ctgttgagcg aaaaggaaat tccaaaagtg
    18481 gtgaaggaac tcgattacga caacgtagta gggcaggaca gcctcactcg tttcgatagc
    18541 gagaagcgta aaaagaagaa gaaaaagaag aaaccaggtt caggaattaa ttccggttct
    18601 gaagctacgc cggtaagtgc tgcagcgcct caaacctcga cacctgcgaa agttcaacgt
    18661 cctatccagc ctaacaggcc gcaagttcaa cgtccgccac aagcgaatca agcgaatcag
    18721 caaaatcgcc cgcaggttca acgtccacca cagacaaacc cgcctaacca gcaaaatcgt
    18781 ccgccacaac agcgcccgaa taatcccaat aataacaaac gcccgaatca tggtccgcgc
    18841 cctcctaagc cgccacagac tcccgaagcc tgagctattc ctattcttgc ttttggtgtt
    18901 attcagcagc tcttgcgata agcaacgggt attcgaagaa aacacgccta ttcccgataa
    18961 tatctggaaa gccgatcagc ccgtgcgttt cgaagttact atcgacgata cactgagcgg
    19021 acataatttc ctcatgaaca tccgccatgc cgaaggatat ccctacagca atctattcgt
    19081 gttcctaaaa accgatttcc ccaacggcaa ggcagcacgt gatactattg agtttattct
    19141 gcaaaatccc gatggaaaat ggagcggttc cggtctcggc gatttgtggg atagccaaat
    19201 tcaattcaag caaaacatcc gttttcccct caaaggaaaa tacaccttca ccattgaaca
    19261 agccatgcga gctcctgccc ttcctatgat catggaaatg ggtttacgca tcgaaaaata
    19321 ctcggctgaa taatccgact tctagtttaa gttattgttt ttggggcttg ccctgtcctg
    19381 aaaaaggcca gtgccgcgtc atcgctcata ctccgttcct ccttcgtcgg agccggggta
    19441 atccgctcta ccgtaaaccc cttcggggct aagccagcat actgctttat taaagctcaa
    19501 tgctcctgag aaaaactggg tgaacatccg aaatcgaatg ctgcttcatc aaagctcgat
    19561 aaagtgaagc tcgatttgga aattactatt ggttaacgta atcactcctg gcgcactagg
    19621 tgtagcagtt gccggattca aattaaacgt agccattaac tctgatgacg gttgaagtaa
    19681 tgtaccatca ttgtccaaca tctgaattat ataatcgttg ttagctaaaa taggcttact
    19741 aaccgagctg ctaaaatccc aaaaaacagg acattgattt aattgtaatt cgttgatagg
    19801 aggattaatc gtacttcgca aagcactacc tgcaacagca tattcgaaaa aaacatctgg
    19861 ccaatttcct ggagcaacat cccaagtggt tcctgatata ttattaatat taaacgagtt
    19921 aacctgtaaa cgagtaagat aacgtgtttt tagtgggtca acattaatga taaccgacgt
    19981 gcgatcccat ttcttgtctt ttttcgacaa cgccttcatt tccaccaaat aaactcccgg
    20041 ttgagcatac gtatgactgg cttgttcgcc ctcaacttca gctgttccat ctccaaaatt
    20101 ccaaactact cgcttggcat ctaaagcaca gctagtaaat gctaaactct cattttgctg
    20161 aacatgtgta cggtcaactg taatacacgc ctgtggttca ggagtacatg aactaacaag
    20221 taaaataaca gcacaacata gtgacagaaa caaagacttc atagtttcaa aatttgtcgg
    20281 taaaacggta ttcgatggat tttggtattc tccaaagggc aagaatacat aaaagcaaaa
    20341 cgcccaacca aatggtcagg cgtttcgtgt gttattttaa ccttttttaa atcggtcagc
    20401 tacaacattc caattaacgg tattccacca agccgaaata taatccgggc ggcgattttg
    20461 gtagtgtagg tagtatgcat gttcccacac atctaaagca agaataggaa agcctttcac
    20521 atcagctaaa tccatcaaag gattgtcttg attaggagtc gaagtaacag ttaattttcc
    20581 gtcatgcttc actaaccaag cccaaccaga accaaaacga gtagctcctg ctttagcaaa
    20641 gtcttctttg aatttatcaa aagagccaaa cgcttgatta attgcatcag ccaattcgcc
    20701 tgttggagcg ccacctgcat ttgggcccat tatttcccaa aaaagactgt ggttataatg
    20761 tcctcctccg ttgttacgca cggcaggtga aagtttagaa atattcgaca tcaattgctc
    20821 taggctcttt ccttcttcgg cagttcctgt aagggctgcg ttgaggttgt ttacataagc
    20881 ctgatgatgc ttagagtgat ggatttccat cgttcttgca tcaatattag gttctaatgc
    20941 tgcgtattcg taagaaagtt ttggaagttc gaatgccatg ggtttggggt attaaaatat
    21001 ggattgacaa aataacagaa tgtcgggcag atggttgttc attttctaaa gaatctttta
    21061 catgatttac tgatgccctt aaacatcaac accccgccga aaccggaagg gtgctgattc
    21121 gaacaaaaaa taatctttaa ttaaagcatt gtaccatcgt gttctttttc gcctttcttc
    21181 tccattccaa agcgaacttt aactttcaag tacatcagat acatcgcagg aacaatcatc
    21241 aacgttaaga atgtcgcgat aatcaatccg taaatcatcg tccaggctaa tggtccccag
    21301 aatgccacat tatctcctcc taagaaaaag tgtggttcta aatgagcaaa taagccgtta
    21361 aagtcgatgt ttaccccgat tgctagaggt atcaacccta aaatcgcagc cgatgcagtt
    21421 aaaattacgg gtgtcatacg tgtactacca ccttcggcaa tagcttcttc cggactcaat
    21481 ccgcgagcgc gtaactcatc aataaactcg atcaataaga ttccgtttcg aattacaatt
    21541 ccagctaaag cgaaaatccc tacaccggtc attacaatcg agaaagtcat tcctgttaac
    21601 gcatatccga agaatacacc aatcaaggag aaaactaccg ttgagaaaat gataaacggc
    21661 ttaaccaaag agttaaactg agttaccaaa atcaagaaca tcaacgccaa agctcctaag
    21721 aatgccaaac tcaagaagtc ggatgtttct ttttgttgtt cttgctcacc agtcatttta
    21781 acttcatatc cttcaggaac atccaacgaa ggaataatct gatcaatcga acgaacaatc
    21841 tcattagcag tataacccgt taacacattc gacgaaagcg ttaaaacacg acgttggttt
    21901 ttacggttga ttgagctaaa tgctttctca tatttcaccg atgctacagc cgatacagga
    21961 acttgcttaa acattcctgt actcatgtcc aagaaagcaa tttgcatatt caacaaatcg
    22021 tccaccttat cgcggtagct tgaagccaat cttagcgtaa tttcagcatc gtcatctgca
    22081 tcgcggaact tggaaatctc tttcccaaac aatgctgtac gaagttccat cgcaatttga
    22141 gct
//
LOCUS       DAOOQW010000019        21730 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_2950_length_21730_cov_6.874325, whole genome shotgun sequence.
ACCESSION   DAOOQW010000019 DAOOQW010000000
VERSION     DAOOQW010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 21730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21730
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2950_length_21730_cov_6.874325"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            149..1810
                     /locus_tag="PK281_02420"
     CDS             149..1810
                     /locus_tag="PK281_02420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOY48225.1"
                     /translation="MNSNRSRLTLFLPFLLGAAIASGVFIGYFLSGSTSIGKHNSSIS
                     GVGKDNKLNDVLNYIIEEYVDTINTKKLEEDAIVSLLQQLDPHSAYIPAAELQGVSEQ
                     LEGNFDGIGVEFNIQHDTIMVVAAITGGPSESLGIQSGDRIVRIEGKSIAGIGVKNED
                     VIKKLRGKRGTKVKVGIYRPSTRRITDYVITRGQIPIYSVDASYMATPDIGYIKISRF
                     AATTYDEYLKAFNELSMAGMKKLILDLRDNPGGYLNAAVDLCDEFLPKGKKIVYTQGK
                     ARKREDFDATAKGGFESGGLVILIDEGSASASEIVSGAVQDNDRGWVVGRRSFGKGLV
                     QEEVRFDDSSAMRLTIARYYTPTGRCIQKSYKEGSDAYYHELGERYSRGEMASADSMK
                     SMKGIAFKTPAGRVVYGGGGIMPDVFVPIDTSGYSIYLSEIAAKGLINQFVFEYVDSH
                     REEIKKQYKTADSFARSFPIQNVLSGFSAFTIKNGVKSDPASLNRSSEIIRSQILALM
                     GRAIYGNKGFYSILNSRDKAVIKAIELLNSNKLVSNSSNAKPSAF"
     gene            1822..3867
                     /locus_tag="PK281_02425"
     CDS             1822..3867
                     /locus_tag="PK281_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013072256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HOY48226.1"
                     /translation="MKKICLFFVLSLLVFDASAQIQTMINWTFSQKRTNDSTEVLTFV
                     ASMSGGHHLYSQYTPEGGPLPTIFDFTASKEYSRIGKVKEPKPETVHDDLFGVDVSTF
                     KEKKVTFTQEIRIKSGKDFKITGEISGMTCDDQGCIPFDQQPFSFTIKGRTIAGVVTS
                     AIDTSTAQIAPTGDTDAIVQASGPSTGGPDGCKDYVIEGLNSTQAPAEAQSYWFIFLL
                     GFGGGLVALLTPCVFPMIPLTVSFFTKSAQNRRKGLIMAVTYGLSIAAIYTLLAVPFI
                     VAKVPPDTLNEIATSAVLNIIFFVIFVVFSISFFGYFEIQLPSSLANKADSASNLGGF
                     IGVFFMAITLALVSFSCTGPILGSFLAGTLATNPDPYNILFVMLGFGSALGLPFALFA
                     AFPAWLNSLPKSGGWLNSVKVVLGFLELGMALKFLSNADLVEQWGILKREPFLAIWAI
                     LAFGLALYLLGFIRFPHDSKLKKRSAFRIGLATLFFASGVYFTTGIFGAGLELISGFP
                     PPTFYSYAAQSEKKPDASAVAGDEHCPQGIPCEHDYEVAKARAIKENKPLLIDFTGWA
                     CVNCRRMEEGVWIDPEVKKIMSEEYVLVSLYVDEKANLPESEWFTSCKTGKRVRTVGN
                     KWSNMQVVNFNTNSQPQYVALSPEGKQLAEPVGYTSKENYLNFLKSGLSKAKETATR"
     gene            4030..4362
                     /locus_tag="PK281_02430"
     CDS             4030..4362
                     /locus_tag="PK281_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6150 family protein"
                     /protein_id="HOY48227.1"
                     /translation="MRFFLSFILFVFVQQLVAQKVYSVAYESQAQVKVFVVKYESQAD
                     LLVYKVKYDSQAGDNNGKWFFTQYESQAKKKIFFVDYESQADLKVFFVDYETQAGWKN
                     SAKKALMY"
     gene            complement(4427..5842)
                     /locus_tag="PK281_02435"
     CDS             complement(4427..5842)
                     /locus_tag="PK281_02435"
                     /inference="COORDINATES: protein motif:HMM:NF013776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOY48228.1"
                     /translation="MELVGRNKEIIRLNELFERNRSEFVVLYGRRRIGKTFLVRKMFQ
                     NRMSFQLTALANASLSNQLLNFHYSLVQTFGDSSSNKSPSNWLEAFQLLKQGLENLPE
                     GKKLVFIDELPWLDTPRSGFLSAFEHFWNDWASNRNDVLLIVCGSAASWMIGKLLKNR
                     GGLHNRVTERIHLKPFCLAECKAMIESMGLEWSLHQLLEMYQVLGGVPYYWSLLSKGK
                     SATQLIDDLLLGGSALLRNEMQNLYASLFTNYQAHEAIVKALSTQKSGLNRDKLLQLS
                     KLPNGGGTTQILEELEQSDFIRKYSPIGRKSRDSIYQLTDFFTLFHFSFLENSKKATP
                     QSWMSYIDHPRHRAWSGYSFELLCLMHTDQIKRAMGISGIEVNVASWCAKGDKPIQID
                     LLMERRDQVINIFEMKFSIAPYSIDKSYQQELLTKIDTFRRETKTRSAVHLVLVSPYG
                     LQANSYSGTIQLALTGEDLIK"
     gene            6173..7960
                     /gene="lepA"
                     /locus_tag="PK281_02440"
     CDS             6173..7960
                     /gene="lepA"
                     /locus_tag="PK281_02440"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018278301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HOY48229.1"
                     /translation="MKNIRNFCIIAHIDHGKSTLADRLLEYTNTITKRESQAQVLDDM
                     DLERERGITIKSKAIKMEYELNGEKYILNLIDTPGHVDFSYEVSRSIAACEGALLIVD
                     AAQGIQAQTISNLYLALEHDLEIIPVLNKMDLPGAMPEEVKDQIVELIGCKREDILAA
                     SGKTGMGVHDILKAIVDRVPAPKGDPDGPLQALIFDSVFNSFRGIYAYFRVINGSIKK
                     GDKVKFMSTGSEYFADEIGLLRLKPEARNEVKAGDVGYLISGIKEAREVKVGDTITHV
                     DRPCAEQIKGFEDVKPMVFAGIFPVDTEDYEELRYSMEKLQLNDASLTFEPESSAALG
                     FGFRCGFLGMLHMEIIQERLEREFNMTVITTVPNVSYKAYKTNGEELVVNNPTDLPDP
                     SKLDRIEEPYIRAQVITKSEYTGAIMTLCIQKRGMLVNQNYLTTDRVELVFEMPMGEI
                     VFDFYDKLKSLSRGYASFDYHPIDYRESKLIKLDLMLNGEPVDALSSLIHRDNAYDFG
                     KKICEKLKELIPKQQFEIAIQASIGAKVIARETVKAMRKDVTAKCYGGDISRKRKLLE
                     KQKEGKKRMRQVGSVEVPQSAFMAVLKLD"
     gene            8078..8959
                     /locus_tag="PK281_02445"
     CDS             8078..8959
                     /locus_tag="PK281_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013632653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HOY48230.1"
                     /translation="MEISISQQDIQYRIFTIRGIQVIVDRDLAALYGVETKVLNQAVK
                     RNLNRFPSTFRFQLTNNEVLELVTKCDRFENLKHSSVLPFAFTEQGVAMVSAILKSDI
                     AVHVSIQIMETFVQLRQLITSNMGLIQRLDALERKQIVSDNKFDQLFEALESGNLKPS
                     KGIFYEGQIFDAYEFVCEIIRRANSSIIIIDNYIDENVLKLLTKRKEGVKVQLLMKKI
                     SPAFELDIQKYNAQYPTIEAHCFLDAHDRFILIDDKELFHIGASLKDLGKKWFAFSSM
                     PSFANSVIKKIKHDIEL"
     gene            9017..10112
                     /gene="prfB"
                     /locus_tag="PK281_02450"
     CDS             join(9017..9070,9072..10112)
                     /gene="prfB"
                     /locus_tag="PK281_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_152552821.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HOY48231.1"
                     /translation="MIQQDQITSLDDRLDALRGIFDVDRKLSELEFEERETTAPNFWD
                     DPSVAEKKLKAIRDKKVWTDGWKKIGAGIEDLKVLMDFFKEGGATEAELDEQYALIVE
                     DLEDLEFKNMLSGKEDNLSCILTINSGAGGTESCDWASMLLRMYRMWAEKNKFKVSEV
                     DMQEGDVAGIKSATIQIDGEFAFGYLKGESGVHRLVRISPFDSNARRHTSFCSVYAYP
                     LIDDTIDIQINMGDIEWDTFRAGGAGGQNVNKVETAVRLTHKPTGIIIKNQESRSQLQ
                     NKDKALQLLKSQLYEMELRKRQESTDAIEGSKKKIEWGSQIRNYVFHPYKLVKDLRTD
                     KETSDVQGVMDGDLNAFIKAYLMEFGAKHD"
     gene            complement(10142..11926)
                     /locus_tag="PK281_02455"
     CDS             complement(10142..11926)
                     /locus_tag="PK281_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48232.1"
                     /translation="MKQLLLFFLLFPLLLFSQSQGIVQIQHGRVHEQRRATLEDLVRQ
                     SDYIALVQLPTQSPDSLPVSFDDLGTGVYIISFKPIEVYKQTFPLYNEHYLFEHSTNE
                     KLGTDLYRQGERCYVFLNNNEPKTIGDAPDLRLLQNQRGIPSLLLGGLIAEKDIKKKE
                     LKRLCKPSKFVKAAVRGDTNAMKRMARKHVDYLNQFDEGSPVQSFFGFSRWLMGMRGI
                     DGVMLDSCSSHIAIWPGWSDYYYWVNTPEGRKEYLLVVQHGKVFPVPFNYLRHGYSNK
                     WVVKNVYRTDSAREKLIENCERARMYEKQDLYNNYIRFYTEEGRLNWSVYNGPAHLDS
                     EGDLLRVKLKLYNTIDSAMYLRWPGKQNSGKKVIQFALQHQTTRKIYRELSNEVSFIH
                     DETLGPEDRLVIAQDTLSGWFSINDPFLEHTDASAAHRFEKLPPGAYNLMVKYDPYPE
                     AVADTLSWNPFCDSISSAAGYPFWIYADTVSRKFTIEAEVVQRSEPFVNAYGQHGYTS
                     GIVKVLKNDNLHGPAVGTLIAWKLLVNVDYGWTSHSHPPTTVDILSVGSKIELDLIDS
                     NAGGTIKTTSGSYRLYGTPELYDSVRIK"
     gene            12098..13483
                     /gene="fumC"
                     /locus_tag="PK281_02460"
     CDS             12098..13483
                     /gene="fumC"
                     /locus_tag="PK281_02460"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HOY48233.1"
                     /translation="MSFRTEHDTMGAVQVPADKYWGAQTERSRNNFKIGPEASMPKEI
                     IYAFAHLKKAAAQANRDLGVLSADKCDLIGKVCDEILAHKLDDQFPLVIWQTGSGTQS
                     NMNVNEVIAYRAHVLSGGQLTDDKKVLHPNDDVNKSQSSNDTFPTAMHIAAYTQAVEV
                     TIPGMEKLRDTLVKKSADFKDIVKTGRTHFMDATPLTLGQEFGGYAQQVTNSIRAIKN
                     ALEMVKELALGGTAVGTGLNTPKGYDVLVAQKIAESTGYLFVTAPNKFEALAAHDAMV
                     ELSGALKRAAVALMKVANDVRMLSSGPRSGIGEIIIPDNEPGSSIMPGKVNPTQPEAL
                     TMVCAQVMGNDVATSIGGATGHFELNVFKPLIAANVLQSARLLGDACVSFNDKCAVGI
                     EPNLPQIQQHLENSLMLVTALNTHIGYENAAKIAKTAHKQGKTLRQVSIELGLLTDEQ
                     FTAWVDPKKMV"
     gene            13783..16182
                     /locus_tag="PK281_02465"
     CDS             13783..16182
                     /locus_tag="PK281_02465"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY48234.1"
                     /translation="MNMCRNFKTVYLLLASLLMTFKLAIAQAPECQWVNEISGNNIQL
                     VNSCKSDENNNLYSTGLGSSNFNYDCFFNPVNVSFLYPGQTDLILTKTNSIGNLIWAN
                     NISYLGPNGSTIFSSGGEIEYDGSGHLYVSDVFGSTATFHGSNFQDSSISTLSGIGNS
                     SILLAKYDTSGIPVWVRQLDGLGVRTIYNTSISPNGNVAICGSFLNSFILTDSTYVSQ
                     GANDGFVAEFSSTGILLSFHVFGTPEEDFCTDVSYDSNGNLYVSICSSGQSIDADPGS
                     SIYDLVKTATNASYFAVIVKLNSSGNLIWAKKLDSQTGYIVNSIQVDDSDDLLIAGQF
                     DGSLDVDMNEASIYTITCNTPNISVNHFVSKNNSNGDFIWVAHVPTNTFGFVNGYWNL
                     ESDQSNNIYISGLSGSCDFNPGVAIDSISAFPTFTSYLWSLNAQGGHRWVKQVINPTG
                     SGSVTSFSGITIPPNSDIIYGVGGAYVESTFSTASPDYTIFSSGMGGFMAKYGNCPLI
                     IEKETVTHCDSSYVWNGQEYSESGSYFEFFQTEFGCDSVSRLDLQLSQAVSAELIIQS
                     CDPLVINNQTFNSSGLYNQSLISSVGCDSLLTINFTRINSSSATVDVNTCNPVTINST
                     TYNESGEYIQTLTNSKGCDSLLTINVNFEAPSATVFTADSSIFAFGSFETIQWINCSN
                     NDPISGADSASYFPSGEGQFAAIITLNGCSDTTNCKTYKPAVFTCDDLIISPNPTTDF
                     VSFKYPVSEYLIQVFASNGALIRSQLGNASTQIIDFSAMAAGVYVLQIDSCRIKVVRG
                     K"
     gene            16512..17483
                     /locus_tag="PK281_02470"
     CDS             16512..17483
                     /locus_tag="PK281_02470"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HOY48235.1"
                     /translation="MKISHYLLSLSALLVFTSVGFSQGMKKVVAPANNQVKPATNTVS
                     PAAVAPATPSTPASENQSPWTKNMVKIEGDTFSMGAKEETKNASYYEKPLHTVKVNSF
                     YMGKYEVTVAEFEQFVKASNYKTAAESDTLQMVLLNGKTEARKGMNWRYNEIGEKIAD
                     ADKAKMPVTRISYVDAVAYCDWLSKQSGKTFRMPTEAEWEFAAKGGKGSPNYEYACGS
                     SPDACVVFRNNSKDRIQPVGSRQPGSLGIYDLNGNVFEWCSDWYSPDYYKTSPSDNPQ
                     GPSEARQRVIRGGEIISSTESCKNYKRDHRNPKMAFFNVGFRVVMIE"
     gene            complement(17540..18529)
                     /locus_tag="PK281_02475"
     CDS             complement(17540..18529)
                     /locus_tag="PK281_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48236.1"
                     /translation="MKKFVSVIFLGLISLGMQAQDNINEWVDGKKLWVGCVEKPKPKI
                     YKLDLRDDGGGILPYYPALKIKKLALISFYVADEEFTTNRRKSVYSYNVGNYRVTETQ
                     RKSDVYSTDAGFVRKLTQSMYDIYVTSAKEELKKNGLELLELNELFAQNPEAEQIYKN
                     FNPDVSGIGKSLADFGSFLTESASDYKVGNIASVDGYRIFPTDYFDDYKCKQSFGVLA
                     KQLGVDAVVVSIVTLTNEKGKSVSVKSAGSFVFGPNPVAKQDKKYVGLAGAGYNEGFL
                     YWGIQFKHPDGVPFLKMKKGEITSFSLDGFDRLIDLSLNASLGKIWESSQMKK"
     gene            18775..20655
                     /locus_tag="PK281_02480"
     CDS             18775..20655
                     /locus_tag="PK281_02480"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOY48237.1"
                     /translation="MIRLLTLAFLVTFHSLRANDTLQVRKLVDSTMRIKFNDPQTAER
                     LAHQAMQLAKKDTFSRYIGSVYSLFGAIYDDQGNFNQSGHYYDTALVYYQKAHFIPGI
                     ANIHNNFGLIYRKQGLYSMALPAFMKAMHLADSLKSERIQARVSQNIGMLFNDMGRSN
                     EAIPYLERSVKLYTKLELNTFVGRTLNILAAAFELSQKYPSAEKAYLRALDIARSEQD
                     TANLASTLDNYGGLLSTLGKLKEAENAHRMAFQLVNQVQDSASCVLIMNNLANVLIKE
                     NQLPEAIQLAKQVSAICLRINDRPELMRAYRNLADAFEKTGNYKDAATYRSKEALLQQ
                     EIFQSDLADRSAQWDALYQTSQKEAELKEIKQIQAIRSLELAVSQEAVSSSRKTIFIL
                     SLLILVGLGATYVRFRRIRLVQQIRLSEERSKQQQEGLRAILIAQEEERKRIGKEMHD
                     SLGQKLSALKIRLQQHENTSGESLAILSETLDEARNISHLLMPVTLQRMGLKAALQEL
                     CERSFTGTSIEGEFETFALEERLSEEVEIALFRVAQELIANAIKHSGMTKFSLQLYRT
                     KEHVLLVAEDNGKGLELSKSGGMGMRNIQSRIESISGEIRWETAPEGGLLCTVKVPFN
                     SR"
     gene            complement(20642..21295)
                     /locus_tag="PK281_02485"
     CDS             complement(20642..21295)
                     /locus_tag="PK281_02485"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOY48238.1"
                     /translation="MLESTTKHTVAIADDHALFADGIASMLSTLSNVQINERFSDGKQ
                     LCDWLRHHRVDLVLMDFQMPVMDGLQAMKIIQETHPEIKVLMLSMHGNPLLVQEAMKL
                     GARGFLMKSADKEEFQLAVQKILAGGKYFSSELMEKILSNHSTESSNPGETQLTEREI
                     EIVREVAAGLSSQEIADKLFISVRTVETHRKNIMSKLDVKNMAGLVRFALVNGLISN"
     gene            21384..>21730
                     /locus_tag="PK281_02490"
     CDS             21384..>21730
                     /locus_tag="PK281_02490"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HOY48239.1"
                     /translation="MKAFSFLSALLLTISSFAQTSIQTAQEQLVSALNRHCLELTQQL
                     YKPGTNLFCSPYSLSAALAMTYAGASGITATEMEKVMHYPQGLTHEGFLMMSKRMVEI
                     NAKGKVKLAVANA"
BASE COUNT         6524 a         4371 c         4424 g         6411 t
ORIGIN      
        1 tatgctgttt ataaaggcat ggacatcgat gaaggcgttg agtttcttcg gaaattcggt
       61 gttgaggtag atcattatga aatttcgtaa ggttatttaa atcatttcga ctacttttta
      121 cgtttatagc agttcccaac aaaacgcaat gaattcaaac cgctcccgat taacactttt
      181 tcttcctttc cttttgggtg ctgccattgc ttcgggcgta tttatcggtt attttttatc
      241 gggctctact tcgataggaa agcataattc gagtatttcg ggagttggaa aggacaacaa
      301 actcaacgat gtgctcaatt acatcatcga agaatacgtc gataccatca acaccaaaaa
      361 actagaagaa gacgcaattg tttcacttct tcaacaactc gatccacatt cagcctacat
      421 tccagcagcc gaactgcaag gtgtgagtga gcaactcgag ggaaatttcg atggaatagg
      481 agtagagttt aacatccaac acgatacaat catggttgtg gctgctatta cgggtggtcc
      541 atccgaatcg ctcggtattc aaagtggcga tcgaatcgtt cgtatcgaag gaaaaagcat
      601 cgctggaatc ggtgtgaaaa acgaagacgt gattaagaaa ctccgcggaa aacgtggaac
      661 aaaagtcaaa gttggaatct atcgcccatc cacccgtcgc attaccgatt acgttatcac
      721 ccgtggacaa attccaattt attcagttga cgcgagctac atggcaactc ccgatattgg
      781 atacatcaag atttctcgct tcgcagctac tacatacgac gagtacctaa aagctttcaa
      841 cgaactcagc atggctggaa tgaaaaaact cattctcgac ctgcgcgata atccaggagg
      901 atacctcaat gctgctgttg atttgtgtga tgaattcctc ccgaaaggaa aaaagattgt
      961 ctacacacaa ggaaaagctc gcaaacgcga agattttgac gcaactgcaa aaggtggttt
     1021 cgaatcgggc ggattggtca ttcttatcga cgaaggatct gcatctgctt ccgaaattgt
     1081 ttcaggagca gttcaagata acgaccgtgg ttgggtagtt ggtcgccgct cttttggaaa
     1141 aggcttggtt caagaagaag tgcggtttga tgatagttct gctatgcgtt taaccattgc
     1201 tcgttattat actccaacgg gtagatgcat tcaaaaatcc tataaagaag gaagcgatgc
     1261 ttattatcat gagttaggag aacgctattc acgtggtgaa atggccagtg ccgattccat
     1321 gaaaagcatg aagggcattg cttttaaaac acctgccggt cgtgtggttt atggcggcgg
     1381 aggaattatg cccgatgttt ttgtgcccat cgacacaagc ggttattcca tctatctctc
     1441 cgaaatagct gcaaaaggac taatcaatca atttgttttc gaatacgtcg attctcatcg
     1501 cgaagagatt aagaaacaat acaaaacggc cgatagcttt gctcgtagtt ttcctattca
     1561 aaatgtgctt tctggctttt cggcattcac cattaagaat ggagttaagt cagatcctgc
     1621 tagtttaaac cgctcttccg aaattatccg atcacaaatt ctagctctca tgggaagagc
     1681 aatctatggt aacaaaggtt tttattccat cctaaacagc cgtgataaag ccgttatcaa
     1741 agcaatcgaa ctcctgaatt ctaacaaatt agtcagcaat agctcgaacg caaaaccttc
     1801 agcattctga ttcctacgct tatgaaaaaa atctgccttt tcttcgtttt aagtctgctt
     1861 gtttttgatg catctgctca aattcaaacc atgataaatt ggacgttcag ccaaaagcga
     1921 acaaacgata gcaccgaagt tttaaccttt gtggcatcca tgtcgggcgg acatcacttg
     1981 tattctcaat acactcccga aggcggacca ttacctacta ttttcgattt cacggctagc
     2041 aaagaataca gccgaattgg aaaggtaaaa gaaccaaaac cagaaacggt tcatgacgat
     2101 ctttttggcg tagatgtaag tacttttaag gaaaagaaag tcaccttcac acaagaaatt
     2161 cgaatcaaaa gcgggaaaga ttttaaaatt acgggtgaaa ttagtgggat gacttgcgac
     2221 gatcaaggtt gtattccatt tgatcaacaa ccgttttcat tcaccataaa aggtcgcacc
     2281 atcgctggtg ttgtcacttc tgcaatcgat acaagcaccg cgcaaattgc tccaacggga
     2341 gatacagatg caattgttca agcatcaggt ccttcaactg gcggtccaga tggctgtaaa
     2401 gactatgtaa ttgaaggact taacagcaca caagctccag ccgaagctca gagctactgg
     2461 tttattttct tactaggatt tggcggtgga ctcgtggctt tgcttactcc atgtgtgttt
     2521 ccaatgattc cattaacggt tagtttcttc accaagagcg cacaaaaccg tcgcaaaggg
     2581 ctcatcatgg cagtaacgta tggattgtcg attgcggcta tctacacgtt gttggctgtt
     2641 ccgtttattg tagcaaaagt tccacccgat acactcaacg aaatcgctac cagcgcggta
     2701 ttgaacatca ttttctttgt cattttcgta gtattctcca tttcgttttt tgggtatttc
     2761 gaaatccaac taccatcttc actcgcgaac aaagccgata gtgcgtcaaa cttgggagga
     2821 tttatcggtg tattctttat ggcgattaca ctggctttag tgtcgttcag ctgtaccgga
     2881 ccaatcttgg gatctttcct cgcaggaacc ttggccacga atcctgatcc ttacaatata
     2941 ttatttgtta tgcttgggtt tgggtctgca ctcggactac catttgcgct ttttgcagct
     3001 ttcccagctt ggttgaattc acttcctaaa agcggcggat ggctcaattc ggttaaagtg
     3061 gtactcggtt tcttggaatt aggtatggcg ttaaaattct tatcaaatgc cgatctagta
     3121 gagcaatggg gaattcttaa acgcgaacct ttcctagcaa tttgggctat tctagcattt
     3181 ggtttagcac tttatttatt aggttttatt cgttttccac acgatagcaa actcaaaaaa
     3241 cgcagcgcat ttagaatcgg tttagccaca ctcttcttcg caagtggagt ttatttcact
     3301 actggaattt ttggtgctgg actcgaactc atctctggat ttccgcctcc aactttttac
     3361 agctatgccg ctcagtctga aaagaagcct gatgcatcag cagttgcagg cgatgaacat
     3421 tgtcctcaag gaattccgtg tgagcacgat tacgaagtag caaaagctcg tgccatcaag
     3481 gaaaacaagc cattgcttat cgattttacc ggttgggctt gtgtcaattg tcgtcgaatg
     3541 gaagaaggcg tttggattga tccggaagtg aaaaaaatca tgagcgaaga gtatgttttg
     3601 gtttcacttt acgtcgatga gaaggcaaat cttcctgaat ccgaatggtt tacgagctgt
     3661 aaaacaggta agcgtgtccg cactgttggg aacaaatgga gcaacatgca ggttgtaaat
     3721 ttcaacacga attctcagcc tcaatacgta gcgttatcgc ccgaaggcaa gcaacttgcc
     3781 gaaccagttg gttatacgag taaagagaat tatttgaact tcctaaaatc aggtttgagc
     3841 aaagcaaaag aaacagcaac tcgataatga ttagggcgtg cctttcctac cttcgctttt
     3901 agctccggca ggaaagtcag gctttcgcgt cgtaggaact ccgctcaatc cttcacgcaa
     3961 aaaatgaaga actgtcattt ccggtgtgga gttagttcaa aacgctattt tcggttttca
     4021 atatcaaata tgcgcttctt tctttctttt attctatttg tgtttgttca acaacttgtt
     4081 gctcaaaagg tgtattctgt tgcgtatgaa tcacaagctc aagtcaaggt atttgtggtg
     4141 aaatacgaat cacaagccga tttacttgta tataaagtca agtacgactc tcaggcgggc
     4201 gataataatg gcaaatggtt tttcacgcaa tacgagagtc aggcgaagaa aaaaatcttc
     4261 tttgtcgatt acgaaagcca agccgatttg aaggtattct ttgtcgatta tgaaactcag
     4321 gctggttgga agaatagtgc taaaaaggcc ttgatgtatt agggctttat ttttaaaatc
     4381 gaatcttaaa atcgagatta agtgttattc gatagaaggt taaaaattac ttaatcaaat
     4441 cttcacctgt taatgctagt tgtatagttc cgctgtatga attggcttgc aagccataag
     4501 ggctaactaa tacaaggtga acagctgaac gagttttcgt ttctcggcgg aaagtatcta
     4561 tcttggttag taactcttgt tgataggatt tatctattga atagggtgca atggagaatt
     4621 tcatttcaaa tatgtttatc acttggtcgc gtctttccat caataaatcg atttgaatgg
     4681 gcttatctcc tttagcgcac cagcttgcca catttacctc aataccagaa attcccatgg
     4741 ctcgtttgat ttgatctgta tgcattaagc agagtaattc aaacgaatac ccagaccaag
     4801 cccgatgacg tggatggtca atataactca tccaagattg gggtgttgct tttttggaat
     4861 tttctagaaa actgaaatga aaaagcgtaa agaaatcagt taattggtaa atcgaatctc
     4921 ttgattttcg accaattgga ctgtattttc tgataaagtc gctttgctca agctcttcaa
     4981 gtatttgtgt tgttccgcca ccattgggaa gcttactcag ctgaagaagt ttgtcacgat
     5041 tgagtccgct tttttgtgtt gataatgctt tcacgatggc ttcatgggcc tgataattag
     5101 taaacaaaga ggcatatagg ttctgcattt catttcgaag caaggcagaa cctccaagta
     5161 ataaatcatc gattaattgt gtggcacttt taccttttga tagcaagctc cagtaatagg
     5221 gaacaccacc taatacttga tacatttcaa gaagttgatg taagctccat tctaatccca
     5281 tcgattcaat catagcctta cattcggcta gacaaaaggg ttttaggtgt atgcgttcgg
     5341 taactcgatt atgtaaacca cctcgattct ttaatagttt acctatcatc catgaggcag
     5401 cagagccaca tacgattaat agtacatcgt ttctattgga agcccaatca ttccaaaagt
     5461 gttcgaaggc ggataaaaat cccgaacgag gagtgtctaa ccagggtaat tcatcaatga
     5521 atactagctt tttcccttca ggtagatttt ctaagccttg ttttagtaac tgaaatgcct
     5581 ctaaccaatt actgggagac ttatttgaag atgaatcacc aaatgtctga actaagctat
     5641 aatgaaaatt taatagttgg ttcgaaagtg atgcatttgc caacgccgta agttgaaatg
     5701 acatgcgatt ttggaacatt ttccttacca gaaatgtctt tccaattctt cgtcgcccat
     5761 agagaacgac aaattcggag cgatttcttt cgaacaactc gtttaaacga ataatttctt
     5821 tatttctacc gacaagctcc ataatgtgct acaaatataa aataaattta gatatagcga
     5881 attataaata catatttcgc tataaatatc aaagcaaata agatatagcg gaatataaac
     5941 caatttctat tgtccacgtg atacagaaga gagaaaagct tgaaagggta ttcggacaat
     6001 aattgtaacg aaatggcaga ttacgaaatt cataagcttt caacttattg aatcaacctt
     6061 aaaattaatt gaagtgccta ctaactaata acaacagtag gaggttgggc atttagcggg
     6121 caattggtgt atgcttattt caactttggt tttatctatc tttgcagccg caatgaaaaa
     6181 catccgtaac ttttgcatta tcgctcacat cgaccatggt aaatcaacct tggccgatcg
     6241 tctgctcgaa tataccaata ccatcaccaa gcgtgaatca caggcacaag tcctcgatga
     6301 tatggatttg gagcgtgaac gtggaatcac cattaagagt aaagccatta aaatggagta
     6361 tgaactcaat ggggagaagt atatcttaaa cctaattgat actccaggac acgtcgattt
     6421 ctcctacgaa gtttcgcgtt ctattgcagc ttgtgaaggc gctttactta ttgttgatgc
     6481 cgcacaaggt attcaagccc aaaccattag taatctgtat ttggcattgg agcacgattt
     6541 ggaaattatt cctgttctga ataaaatgga tcttcccggt gcaatgcccg aagaagtgaa
     6601 agaccaaatt gtagaactca ttggttgcaa acgggaagat attttagccg cttcaggtaa
     6661 aactggaatg ggcgttcatg atattctaaa agctattgtt gaccgcgttc cagctccaaa
     6721 aggcgatccc gacggaccac tacaagcact aatttttgat tcggttttta attcgtttcg
     6781 tggtatttac gcatacttcc gcgtgataaa tggatcgatt aaaaaaggag ataaagtaaa
     6841 attcatgtca accggcagtg agtactttgc cgatgaaatt ggcttgctcc gattaaaacc
     6901 agaagcacgc aatgaagtaa aagcgggaga tgtaggttac ctcatttcgg gaatcaaaga
     6961 agcgcgcgaa gtaaaagtgg gcgatacgat tacacatgtt gatcgtccgt gtgctgaaca
     7021 aatcaaaggt tttgaagatg tgaagccgat ggtatttgcg ggaattttcc ctgtcgatac
     7081 ggaagattac gaagagttgc gctattccat ggaaaagctg caacttaacg atgcttcact
     7141 cacgtttgaa ccagaatcat ctgcagcgtt aggatttggt tttcgttgtg gtttcttggg
     7201 aatgcttcac atggagatta tccaagaacg cctcgaacgg gaatttaaca tgaccgtaat
     7261 tacaacggtt cccaacgtat cctacaaagc gtataaaaca aacggcgaag aattggtagt
     7321 aaacaaccca accgaccttc ccgacccaag taaactcgat cgaatcgaag agccgtatat
     7381 tcgtgctcag gtgattacaa aatcggaata cacaggtgct atcatgactt tgtgtattca
     7441 gaaacgtggt atgttggtca accaaaatta cctcacaact gatcgggtag agttggtttt
     7501 cgaaatgcca atgggcgaaa tcgtatttga tttctacgac aaactaaaat cgctttctcg
     7561 aggttacgct tcgttcgatt atcatccaat cgactatcgc gaatccaaat tgattaaact
     7621 cgatttgatg ttgaatggtg agcctgttga tgctttgtca tcccttatac accgcgacaa
     7681 tgcgtatgat ttcgggaaga agatttgcga gaagttgaaa gaactcatcc cgaaacaaca
     7741 attcgaaatt gcaattcagg cttccattgg agcaaaagta attgcccgcg aaacagtaaa
     7801 agctatgcgt aaagacgtaa ccgcgaagtg ttatggtggt gatatttcac gtaaacgtaa
     7861 actcctcgag aagcaaaaag aaggaaagaa acgtatgcgc caagtaggct ctgttgaggt
     7921 gccacagtcg gcatttatgg cggtgctaaa actggattaa tagcatatta catattcttg
     7981 agaacccgct cctgcattgc ttgagcgggt ttttgttttt tacattgatt ccattaattt
     8041 tatccattat ctagtttaaa ctttatcgta tgttattatg gaaattagca ttagtcaaca
     8101 ggatattcaa tatcgaatat ttaccattcg cgggattcag gttatagtag atcgagacct
     8161 cgcagcactt tatggtgtag aaaccaaggt actaaaccaa gctgtgaagc gaaatctcaa
     8221 tcgcttccct tcgactttcc gatttcaact cactaataat gaggtgcttg aactggtcac
     8281 aaaatgtgac cggttcgaaa atctgaagca ttcatctgtt ttgccttttg catttacaga
     8341 acaaggagtt gctatggttt cagcaattct taaaagcgac atagcggttc atgtaagcat
     8401 tcaaattatg gaaacatttg tacagttgag gcaattgatt acttcaaaca tgggtttgat
     8461 tcagcgcctt gatgcacttg aacgaaagca aattgtttca gacaataaat tcgatcaatt
     8521 atttgaagcg cttgaatctg gaaatcttaa accttctaaa ggaatctttt atgagggaca
     8581 aatattcgat gcttatgaat ttgtttgtga aataattagg cgagctaatt cgtcaataat
     8641 catcatagac aattacatag atgaaaacgt acttaaacta ctcactaaaa gaaaagaagg
     8701 agtgaaagtt caattgttaa tgaaaaagat atctcctgca tttgaactgg atattcaaaa
     8761 atataatgct caatatccaa cgattgaagc gcattgtttt ctggatgctc atgatcggtt
     8821 tatacttatt gatgataaag agttatttca tattggtgct tcacttaagg atttaggaaa
     8881 gaagtggttt gccttttcaa gcatgccctc atttgcaaat tctgtaatca agaaaattaa
     8941 acacgatata gaattgtagt tcattccttt ctattgccac aattcctaac tccgaacgat
     9001 tatctttgcc gccattatga tccaacaaga ccaaattacc tcgctggatg atcgcctgga
     9061 tgcgctaagg aggcatcttt gacgtcgatc gtaagctctc tgaattagaa tttgaagaac
     9121 gcgaaacaac cgctcccaat ttctgggacg acccatcagt tgctgaaaag aaactgaaag
     9181 ccattcgcga taaaaaagtc tggaccgacg gctggaaaaa aatcggtgct ggaatagagg
     9241 atttaaaagt tctcatggat ttcttcaaag aaggcggcgc taccgaagct gaactcgatg
     9301 aacagtatgc cctgattgta gaagatctgg aagacttgga gttcaaaaac atgctttcgg
     9361 gtaaagaaga taatcttagc tgcatcctca ccattaactc gggtgcaggt ggaactgaaa
     9421 gctgtgactg ggcttctatg ctcttacgta tgtatcgtat gtgggcagag aagaacaaat
     9481 tcaaagtgag cgaagtcgac atgcaagaag gcgatgtggc cggaatcaag tctgctacca
     9541 tccaaattga tggtgaattt gcctttggat atctgaaagg agaaagtggc gtgcatcgtc
     9601 tcgttcgaat ttcaccattc gattccaatg ctcgtcggca tacctcattt tgctccgttt
     9661 atgcttatcc attgattgat gataccatcg atatccaaat caacatgggc gatatcgaat
     9721 gggatacctt ccgtgcaggc ggggcaggtg gacagaacgt aaataaggta gaaaccgcag
     9781 tacgattaac tcacaagcca actggtatca tcatcaaaaa ccaggaaagc cgttcgcagc
     9841 ttcagaacaa agacaaagca ttacaactcc tcaaatcgca gctctacgaa atggaattgc
     9901 gcaaacgtca ggaaagtacc gatgcaattg aaggttcgaa gaagaaaatc gagtggggtt
     9961 ctcaaatccg caactatgtt ttccatccat ataaactggt gaaagactta cgtaccgaca
    10021 aagaaacctc cgatgttcaa ggcgtaatgg atggtgattt gaacgccttc atcaaagcgt
    10081 atttgatgga atttggcgct aagcacgatt gattctaaaa tccccggact gaggtccggg
    10141 gttacttgat tcttactgaa tcgtatagct ctggtgttcc atataagcga tagctgcccg
    10201 atgttgtttt aatggttcct ccggcattgg aatcaattaa gtcgagttct atcttacttc
    10261 caacacttag aatgtccact gtggtaggtg gatgagaatg tgaagtccaa ccataatcta
    10321 cattcaccaa tagtttccag gcgattagtg ttcctacggc aggtccgtgg agattgtcgt
    10381 ttttgagtac tttcactatt ccgctggtat atccatgttg gccatatgca tttacaaatg
    10441 gctcagagcg ttgaacaact tctgcttcta tcgtgaattt tcgggaaaca gtatctgcat
    10501 aaatccaaaa aggatatccg gctgctgagc taatgctgtc acagaaagga ttccagctta
    10561 gggtatccgc aacagcttcg ggataaggat catatttcac cattaaatta tatgcacctg
    10621 gcggaagttt ttcaaaccga tgtgcagcag atgcatccgt atgttccaag aatggatcat
    10681 tgatgctgaa ccaaccagag agcgtgtctt gtgctatgac gagtcggtct tctggaccaa
    10741 gcgtttcgtc atgaataaag ctcacttcat tacttaattc acgatagatt tttcgagtgg
    10801 tttggtgttg aagagcaaat tgaatcactt tctttccgga gttttgtttt cccggccaac
    10861 gcaagtacat cgccgaatcg atggtgttat acaatttcaa tttcacgcgc agtaaatcac
    10921 cttcagaatc taaatgtgct ggcccattgt aaaccgacca attcaatctg ccttcttcgg
    10981 tgtaaaaacg aatgtaattg ttgtatagat cctgcttttc atacatacga gcgcgttcgc
    11041 aattctcaat taatttttcc cttgcgctat cggttcgata tacatttttc accacccatt
    11101 tattcgaata accatggcgg agatagttaa atggtactgg aaaaaccttc ccatgttgaa
    11161 caactaggag atactctttt cttccttcag gtgtgttcac ccaataataa taatcactcc
    11221 agccgggcca aatagcaatg tgagaggaac agctatctaa catcactcca tctattcccc
    11281 gcattcccat gagccaccta gagaaaccaa aaaatgattg cacaggagag ccctcatcaa
    11341 actgatttaa ataatccaca tgcttacgtg ccattcgctt catcgcattc gtatcgcctc
    11401 ttaccgctgc tttcacaaac ttagaaggtt tgcacaagcg ttttagctcc ttttttttga
    11461 tgtctttttc agcaattaat cctcccaata acaaactcgg aatacctcgt tggttttgaa
    11521 gtaagcgcaa atcgggcgca tcgccaatgg ttttaggttc attgttattt aaaaacacat
    11581 agcagcgttc gccttgtcga tacagatctg tcccaagttt ttcattggta gaatgctcaa
    11641 acaaataatg ctcgttgtat aagggaaacg tttgcttgta aacttcaatt ggcttgaaac
    11701 tgatgatgta aacccctgtt cctaaatcat caaatgaaac gggcaatgaa tcgggcgatt
    11761 gggttggaag ttgaaccaac gcgatataat ccgattggcg aactaaatct tctagcgttg
    11821 ctcttcgttg ctcatgcaca cgcccatgtt ggatttgtac aattccttgg ctctgagaaa
    11881 agagcaataa tggaaagagt agaaaaaaaa gcagcagctg tttcatgaaa atcaatctaa
    11941 gaaaatgcgc ttttcatatt caacattcgc ttaaatgttc accaatataa gctgattttt
    12001 aagtcccttg aaaaccttgt cattccatac tgcttttcta attttgcggc gctgattttt
    12061 aaaaagaaac agctttaacc atcctaattc aaacaccatg tcatttcgta ccgaacacga
    12121 cacgatggga gccgttcagg ttcctgcaga taaatactgg ggcgcacaaa cagagcgttc
    12181 tcgcaacaat ttcaaaattg gtcctgaagc aagtatgccg aaggagatca tctacgcttt
    12241 tgcccatttg aagaaagcag ctgcccaagc aaaccgcgat ttaggcgtgt taagcgctga
    12301 taaatgtgac cttatcggaa aagtatgcga cgagatttta gcgcacaaac tcgatgatca
    12361 gtttccatta gttatctggc aaactggttc gggaacacaa agcaacatga atgtgaatga
    12421 ggtgattgca taccgtgcac atgtgttgag tggcggacaa ttgaccgacg ataaaaaagt
    12481 acttcatcct aacgacgatg tgaataaaag tcagtcgagc aacgatactt tccctacagc
    12541 tatgcacatt gcagcatata cacaagcggt tgaggtgact attccgggga tggaaaaact
    12601 gcgtgatact ttggtgaaga aatctgctga tttcaaagac atcgttaaga caggacgtac
    12661 gcatttcatg gatgctacac cattaacatt gggtcaagaa tttggcggat atgcacaaca
    12721 agtcaccaat tctatccgtg caattaaaaa tgcattggag atggtgaagg aattagcttt
    12781 gggcggaacg gctgttggaa caggcttgaa tactccaaaa ggatacgacg ttttagtagc
    12841 acagaaaatt gctgaatcga ctggttatct tttcgtaaca gctcctaaca aattcgaagc
    12901 attagcagct cacgatgcga tggtagaatt atcaggtgct ttgaaacgtg ctgctgttgc
    12961 cttaatgaaa gtagcaaacg acgttcgtat gttgagctct ggcccgcgct ctggaattgg
    13021 cgaaatcatc attcctgaca acgaaccggg atcttccatc atgccaggaa aagtaaaccc
    13081 aacacaacca gaagcattga caatggtatg tgctcaagta atgggaaatg atgtagctac
    13141 ttcgattggt ggggcaactg gtcatttcga attgaatgta ttcaaaccac ttatcgctgc
    13201 aaacgtattg caaagtgctc gtttgcttgg agatgcttgt gtttcattta atgataaatg
    13261 tgctgtgggt attgaaccaa accttccaca aatccaacaa catttagaga attcactcat
    13321 gttggtgaca gctttaaaca cgcatattgg ctatgaaaac gcggctaaaa ttgcaaagac
    13381 agctcacaag caaggtaaaa cgcttcgtca agtttccatt gaattaggat tgctaaccga
    13441 tgagcaattc actgcttggg ttgatcctaa gaaaatggtt taacccgaat agtttaaact
    13501 tgatatttat cgtttagaaa aactcaaggt gtttggtagc caacaaacga acagcttcga
    13561 gtaatttgag caaaacgatt taaatccctt aagcagattc ttgattgatt tgaacatcat
    13621 tcaagagtct gctttttcat tgtatgaagt tggttgtcaa gattgttcaa cattaatcta
    13681 gtgttagaga aagcataaat tgtattggct tcacttgtag tataatagaa gaacttgaac
    13741 taattcgata ctttaattca gattaatatc tatattgact gcatgaatat gtgcagaaac
    13801 ttcaaaaccg tctatttgtt attggcaagt ctgttgatga cgttcaaatt ggcaatagca
    13861 caggcgcccg aatgccagtg ggttaatgaa atttcaggaa ataatatcca attagtaaat
    13921 agttgcaaaa gcgatgaaaa caacaatttg tattcaactg ggctaggttc gtcaaacttt
    13981 aattatgatt gctttttcaa cccagtaaat gttagttttc tttaccctgg acagactgac
    14041 cttatcttaa caaaaacaaa ctccatcggt aatttaattt gggccaataa tatttcctat
    14101 ttagggccta atggttcaac gatatttagt tctggaggag aaatcgaata cgatggttca
    14161 ggtcatttat atgtttccga tgtgtttggt tcaacggcaa cttttcatgg tagcaatttt
    14221 caagattcaa gtatttcaac cctttcgggt atagggaata gcagcatttt attagcaaaa
    14281 tacgacactt ccggaatacc tgtttgggtt agacaattgg atggattagg cgtaagaaca
    14341 atttacaata cttcaatcag tccaaatggg aatgtcgcaa tctgcggatc ttttttgaat
    14401 tcatttattt taactgattc aacatatgtg tctcaaggag caaatgatgg atttgttgct
    14461 gaattttctt caacaggcat tctgctctca ttccatgttt ttggtacacc tgaagaggat
    14521 ttttgcaccg atgtgagtta tgatagtaac ggaaatttat atgtgtccat ttgttcttct
    14581 ggtcaatcca tagatgctga tccgggaagt tcgatttatg atctagtgaa aacagctacg
    14641 aacgcaagct atttcgcggt tatagttaag ttgaattcaa gtggcaattt gatttgggct
    14701 aaaaaattag attctcaaac aggttatatt gtgaattcaa tccaagttga tgattcggat
    14761 gatttattaa ttgctggaca attcgatggc agtttggatg tcgatatgaa tgaagcttct
    14821 atctatacca ttacttgtaa tacaccgaat atttcggtca atcactttgt ttcgaaaaac
    14881 aattctaatg gcgattttat ttgggttgcc catgttccta ccaatacgtt tggattcgtg
    14941 aatgggtatt ggaatctgga atcagatcaa tcgaataata tctacatttc aggtttgtct
    15001 ggttcatgcg atttcaatcc tggtgtagca atcgattcaa tttctgcatt tccaacattt
    15061 acatcctatt tatggtcatt gaatgcgcaa ggtggccatc gttgggtgaa gcaggttatt
    15121 aacccgacag gttcaggttc agtcactagt ttcagtggca taacaattcc tcccaatagc
    15181 gatattattt atggagttgg aggcgcttat gtcgaatcga ctttcagcac cgcttcaccc
    15241 gattatacca tttttagcag cggcatgggg ggcttcatgg caaaatacgg taactgccca
    15301 ctaatcatcg aaaaagaaac tgttacccac tgtgattcca gctatgtatg gaatggtcag
    15361 gaatactccg aatcaggtag ttattttgaa ttcttccaaa cagaattcgg ttgtgatagc
    15421 gtttcccggc ttgatcttca gttaagtcaa gccgtttctg ccgaattaat tatccaaagc
    15481 tgcgatcctc ttgttataaa caatcaaacc ttcaattcta gcggactata caatcaatca
    15541 cttattagtt cggttggttg cgacagttta cttaccatta atttcacaag aattaactca
    15601 agttctgcta ccgttgatgt taatacgtgt aatccggtta cgattaattc tactacatac
    15661 aatgaaagtg gcgagtacat tcaaacatta acaaattcaa aaggatgtga tagtttactt
    15721 accataaatg tcaattttga agcaccatca gccaccgtat ttacagctga ttcatcaatt
    15781 tttgcatttg gatcttttga aactattcaa tggataaatt gcagtaataa tgatcctatt
    15841 tcaggtgccg attctgcctc ttatttccca tctggagaag gtcaatttgc tgcaattatt
    15901 accttaaatg gctgtagtga tacaacaaat tgtaagacat ataaacccgc agtttttacc
    15961 tgcgacgatt taattatctc tcctaatcca acaaccgatt tcgttagttt taagtatcct
    16021 gtatcagaat acttgattca agtttttgct tccaatggag ctttaattcg ctctcaatta
    16081 ggaaatgcat caactcaaat aatcgacttt agtgctatgg cggcaggtgt gtatgtttta
    16141 cagattgatt cgtgtagaat aaaagtcgtt cgaggaaagt aaaagcacaa acatcctgtt
    16201 tctaatacga gaatgctaaa acgttctcgt tagaacagga ataattcgat attgaactct
    16261 ttatcccgca aatcattcaa cagaaaagaa ctttcattag ctgaatgatg ctctcaaaaa
    16321 gcataacatt tcgtcaaaca tccaatgaat taggattgct taccgatgag caattcaccg
    16381 cgtgggttga tcctaagaaa atggtgtagt tgttttttat ctaattacga atcacctcag
    16441 ctattttgaa aaatctaact ggggtgattt tttttgaaaa tttgtagctt gccctatcaa
    16501 acaataacgc catgaaaatt tcacattact tgctatctct ctctgcatta ttagtcttta
    16561 ctagtgtagg tttttctcaa gggatgaaaa aagttgtcgc acctgctaat aatcaagtaa
    16621 aacctgcaac aaatacagta agcccagcag cagtggctcc tgcaacacca tcaacacctg
    16681 cttctgagaa tcaatctccg tggactaaaa acatggtcaa aattgaaggt gacacattca
    16741 gcatgggagc caaagaggaa accaagaatg cgagctatta tgaaaagcca cttcacacgg
    16801 taaaggtgaa ttcattttac atgggtaaat atgaagtaac agttgctgaa ttcgaacagt
    16861 ttgtaaaagc aagcaattat aaaacagctg cagaaagtga tacactacaa atggttctgt
    16921 tgaacgggaa aacagaagcc cgaaaaggta tgaattggcg atacaatgaa atcggtgaga
    16981 aaatagctga tgcagacaaa gctaaaatgc ctgttacccg aataagttat gtcgatgcag
    17041 tggcttattg tgattggtta agcaagcaga gcggaaaaac attccggatg cctaccgaag
    17101 cagaatggga atttgctgca aaaggcggaa aaggaagccc gaattatgag tatgcatgtg
    17161 gatcatctcc tgatgcatgt gtagtattcc ggaataactc gaaagatcgt attcaacctg
    17221 ttggcagtcg tcaaccgggt agtttgggaa tttacgattt gaacgggaat gtgtttgaat
    17281 ggtgttccga ttggtacagc cccgattact ataaaacctc accatccgac aatcctcaag
    17341 gtccatcaga agcgcgtcaa cgggtgattc gtggaggaga aattattagt tcaactgaaa
    17401 gctgcaaaaa ctataaacgc gatcatcgta atccgaaaat ggcatttttc aatgtgggtt
    17461 ttcgtgtggt gatgatcgaa tgataggaaa gtttgaaaaa agaaagggca gcacatatcg
    17521 gaactgccct tttttgttgc tatttcttca tttgagagct ttcccaaatc tttcctaggc
    17581 ttgcattaag tgataaatct atcagtcggt caaatccatc caagctaaaa gaagtaatct
    17641 ctcctttttt catcttcaaa aatggaacgc catcgggatg tttaaactga attccccaat
    17701 acaagaatcc ttcattatat cctgcaccag ctaagccaac atatttttta tcctgcttcg
    17761 caacgggatt cggaccaaaa acgaaactgc cagcactctt cacacttacc gattttcctt
    17821 tttcatttgt aagcgttact atactcacta caactgcatc aactcctaat tgcttagcaa
    17881 gaactccaaa ggattgtttg catttataat catcaaaata atcggttgga aaaatgcgat
    17941 aaccatctac gctggcaatg tttcctactt tgtaatcgct ggcactttcg gttaaaaagg
    18001 atccaaaatc agccagactt ttcccaatgc ccgatacatc tggattgaag ttcttgtaaa
    18061 tctgctcagc ttccggattt tgagcaaata attcattcaa ttccaaaagc tcaagtccgt
    18121 ttttcttcaa ttcttctttg gcagaagtca cataaatatc atacatcgat tgtgttagtt
    18181 ttctaacaaa acctgcatca gtgctgtaaa catccgattt gcgctgcgtt tcagtaacac
    18241 gataatttcc cacattgtag ctgtaaaccg attttcttcg gttcgtagta aattcttcat
    18301 cggctacata aaaactaatt aatgcgagtt ttttaatctt aagtgccgga taatagggca
    18361 aaatcccacc tccatcatct ctcaaatcga gtttgtaaat tttaggtttg ggcttttcta
    18421 cacatccaac ccacagtttt ttcccatcaa cccattcgtt gatattgtct tgagcttgca
    18481 tccccaatga aatcaagcca aggaagatta ctgatacaaa ttttttcatg gtccgtttgt
    18541 tttacagacc gccaacgtga acgattcaga ccgaacggac agcaggctgg ctcacgaagg
    18601 cggctgatgt gatgaatttt gattcgatgt gtccgttgat gcaaaattca cccagtaaga
    18661 tttcctattt ctacctgaga ttgcgggttt catctaagta ttttcacgta ttcagcagca
    18721 atatcgtttt tcatcgaagc atttttctca ttttagtcct gctcataatt cttcatgatt
    18781 cgactgctaa cgctagcttt tcttgtcaca tttcattcgc tcagggcaaa cgatacgctt
    18841 caggttcgaa agctggtcga ctcaaccatg cgaatcaaat tcaacgatcc tcaaacagct
    18901 gaaaggctgg ctcatcaagc catgcagctg gctaagaaag acacgttttc acgatacatt
    18961 ggttcggttt actccctttt tggggcgatt tacgatgatc aaggaaattt caaccagtca
    19021 ggtcattatt atgacaccgc tttggtctat taccaaaaag ctcattttat tccgggaatt
    19081 gccaatattc acaacaactt cgggttgatt tatcgcaagc agggacttta ttccatggca
    19141 ttgcctgctt tcatgaaagc catgcacctg gcggatagtt tgaaaagtga acgtatccaa
    19201 gcacgtgtgt cacaaaatat tggaatgttg tttaacgata tggggcgttc aaatgaagcg
    19261 attccttatc tcgaacgaag tgtaaaactc tatactaaac tggagctcaa cacctttgtt
    19321 ggtcgcactt tgaacatact tgctgcagca ttcgaactct ctcaaaaata tccttctgcc
    19381 gaaaaagcct acctgcgagc gctcgacata gctcgttccg aacaagacac agctaatctt
    19441 gccagcacat tagataatta tggaggatta ttatccactt taggaaagct taaagaagct
    19501 gaaaatgccc accggatggc gtttcagtta gtaaatcagg tgcaggacag cgcatcctgt
    19561 gtgcttatta tgaataatct agcgaatgtg ctgattaaag aaaatcaact acccgaagcc
    19621 atacaattag ccaagcaagt ttcagccatt tgtctgcgaa ttaatgatcg tccagagttg
    19681 atgcgcgctt atcgtaacct ggcggatgcg tttgagaaaa ctgggaatta caaagatgct
    19741 gctacttatc ggtcgaaaga agccctttta caacaagaga ttttccaatc cgatttagcc
    19801 gaccgatctg cacaatggga cgcactttat caaacctcac aaaaagaagc agaactcaaa
    19861 gaaatcaagc aaatacaggc tattcgttct ctggaattgg cggttagtca ggaagcggtt
    19921 tcttcatcac gcaaaaccat ttttatcctg agtttgctca tactcgttgg attgggagcc
    19981 acgtatgtga ggtttagaag aatacgtttg gttcaacaaa ttcgattatc cgaagaaaga
    20041 agtaagcaac aacaagaagg tttgcgggcc attttaattg ctcaagaaga agaaagaaaa
    20101 cgcatcggga aagaaatgca cgatagcctc ggacagaaac tgagtgcctt aaaaattaga
    20161 ttgcaacaac acgaaaatac atctggtgaa tcgttagcta ttttgtctga aacattggat
    20221 gaagctcgaa acatttccca tttgttaatg cccgttacat tgcagcgaat gggcttgaag
    20281 gcagctcttc aggaattgtg cgaacgtagt tttacgggta cttcaatcga aggagaattt
    20341 gaaacgtttg ctctggaaga acgactttcc gaggaagttg agattgctct ttttcgggta
    20401 gctcaggagt taattgctaa tgcgattaaa cattcaggca tgactaaatt cagtcttcaa
    20461 ctttatcgta ccaaagagca tgttttactg gttgccgagg acaacgggaa aggactagaa
    20521 ttgagcaaat cgggtggaat ggggatgcga aatattcaaa gtcggattga aagtatttcg
    20581 ggtgaaattc gctgggaaac ggcaccagaa ggcggtttgc tttgtactgt aaaagttccg
    20641 tttaattcga gatgagtccg tttactaaag caaatcgcac caagcctgcc atattcttta
    20701 catcgagctt gctcatgata ttcttacggt gtgtttcaac cgttcgaaca ctaatgaaaa
    20761 gtttatccgc aatttcctga ctcgataatc cagctgcaac ttcccgtacg atttcaattt
    20821 ctcgttctgt aagttgcgtt tctcctgggt tagatgattc tgtcgaatga ttggaaagta
    20881 tcttctccat caattcactc gaaaaatatt ttccgccagc taatattttc tgcacagcta
    20941 attggaattc ttccttatca gccgacttca tcaaaaatcc tctcgcacct agcttcatcg
    21001 cttcttgcac cagcaaagga tttccgtgca tactcaacat cagcaccttt atttcaggat
    21061 gcgtttcttg tatgattttc attgcttgta aaccatccat caccggcatt tgaaaatcca
    21121 tcaacaccaa atcaacacga tgatgtcgca accaatcgca gagctgcttt ccatcgctaa
    21181 atcgttcatt aatttgcaca ttggaaagag tcgaaagcat ggatgctatt ccatctgcaa
    21241 atagtgcatg gtcatcagca atagcaactg tatgtttagt agttgattcc aacatgcccg
    21301 caaattaccg attatctgaa atcattttgg acaagattga acttctattt caatttcgca
    21361 taaggcaaaa aaatcctcca atcatgaaag cgttttcttt tctgtcggca ctcttgctga
    21421 cgatctccag ctttgctcaa acatccattc aaacggcaca agagcagctt gtttctgcac
    21481 tcaatcgaca ttgcctcgaa ttaacacagc agctctacaa acccggaact aatctttttt
    21541 gctcacctta tagtttaagc gcagcacttg ccatgaccta tgctggagct agtggaataa
    21601 ctgctactga aatggaaaaa gtgatgcatt atcctcaagg cttaacacac gaaggtttct
    21661 taatgatgtc taagcgaatg gttgaaatca atgctaaggg taaagtcaaa ttagctgtag
    21721 caaatgcctt
//
LOCUS       DAOOQW010000020        21687 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_2957_length_21687_cov_5.043500, whole genome shotgun sequence.
ACCESSION   DAOOQW010000020 DAOOQW010000000
VERSION     DAOOQW010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 21687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21687
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2957_length_21687_cov_5.043500"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3741)
                     /locus_tag="PK281_02495"
     CDS             complement(<1..3741)
                     /locus_tag="PK281_02495"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HOY48240.1"
                     /translation="MMKHYLLIISCFFLFSISSLYSQTEKLIPPSAAKITWADTIHGD
                     IRQDEYHWMRQKTEPQLINHLYAENGYSDRVMAGSSLLMKKMFEEMRVRIPDQTESLP
                     SRMKKYLYFTRYEKGMDYPAYFRKADSLNAPEVPLINFNEIAKTYNYFNISALGISPD
                     NQKMYYGIDTKGDRIFNLFFKDILTGSVLTDTLKNINGICWMGNDAVLYTIPEPKTKR
                     SYRVYKHILGQNTIQDEFLFEEKDVTFNIGLSRSDSERFIFLNTSQTMANEVYVMDLN
                     DAQPQFKLVEPRRRGHQYGLEHYEGNEFIISSDDQAPNLQILTCPISNSRQKNWKVLV
                     PHRKDVMIANSVLAGGKLLVVEQFDAQQKIAVLDLKDNSSYYLPSSTEPASMSLGGYY
                     EYNESKVRYSFTSMRFPSEVWDYDLNTGERTLIRRDTLKGGYDPEKYSSERIYAIAAD
                     SARIPITIIYKKGLQKNGSNPAWLQSYGSYGNPSLPGFSSSNISLLERGFVVAVAHIR
                     GGNDCGRLWYENGKLFKKKNTFTDFISAAEKLISDGYTKSGKLAIQGGSAGGLLMGAV
                     VNMRPDLFGAVVANVPFVDVINTMLDASLPLTTFEYDEWGNPNDPAYYAYMKSYAPYE
                     NVSAQAYPHMLVTGGYNDSQVPYWEPSKWVARMRSLKTDTNLLLLKINMEAGHGGNSG
                     RYGAMKDRAFEMAFVLRSLGIKEEYLQIKGKTTDIQGNELPFVNVFIEGTSIGTASNE
                     NGNFSLDLKQENNQNLVASSIGFKKKVLPITISTDVQDLRIALEPENYQIKTFEVKAN
                     AKDPAYAVMKNAIRTRKEHLEQVDAFSSDIYIKGSVRLNEIPKKRPIFISKENMPDST
                     DLGLVYLSESVARYHVRQPSDYKEEMIASKVAGMKQGFSWNRVSDVLMNFYENLLEFD
                     YYSERGFVSPVSESAMFYYKFKMIGTFVDNGKTINRIALEPKRKNDPCFRGEIFIVDD
                     SWHIHSVDLFLTKDAQIQFVDTLYIKQEYTALEDSIWMPLSMQLMSHIKFFGFGAVDK
                     SVGVFSNYKLHPVYPKNFFGNEIFRIEEKANKKDTVYWTETRPMTLTSEEEKNYRKAD
                     STEIIQTSKPYLDSLDKVNNRFNPAKFIVGGYTHRNSFTKSYLGINPLITMLSYNVTE
                     GVYVQPRIRFSKSYEDNRSWFVEGVGRYGLSDERLKGGLKAWTMTKRTKFNWWYLEVG
                     RFVSQVNGDDPISPLVNACYSLFDKQNLIRTFEKTGATISWNR"
     gene            complement(3814..4344)
                     /locus_tag="PK281_02500"
     CDS             complement(3814..4344)
                     /locus_tag="PK281_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48241.1"
                     /translation="MSSHFLVENITALTLPLSEHPYVLILHADKQPVHVAFIFQRHYF
                     SLTYKGAEVLVDMEQRLKMIRIRKIPCLFLQLKPTSNPIQQKEITDIFLAYPPLQSND
                     TCLSPVWDVLKLSYGLEQGQSLIFGLIEALEVANLIEQKSAYYLPISVEGSFVMPRYS
                     SESVQQRILELKSNNS"
     gene            complement(4341..5135)
                     /gene="dapF"
                     /locus_tag="PK281_02505"
     CDS             complement(4341..5135)
                     /gene="dapF"
                     /locus_tag="PK281_02505"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HOY48242.1"
                     /translation="MLIEFQKYQATGNDFILIDDRDRKFNVQNSSLIQTLCHRKFGIG
                     ADGLILFRSEPGLDFRMIYFNADGYEGSMCGNGGRCAAAFAAQFFPDKSKFMFNAYDG
                     LHSAELIANKDAGYIVRLQMQQVALPEIIESDLLLNTGSPHYVKFVQDSGMVDVPIEG
                     RLIRNSSRFLREGVNVNFAEVHRDSLTVRTYERGVEDETLSCGTGVTASAIASFVMGH
                     IQVQPVKVFTRGGELQVSFIPSPEGDFTDVFLTGEAMPVFTGQIEV"
     gene            complement(5135..5815)
                     /locus_tag="PK281_02510"
     CDS             complement(5135..5815)
                     /locus_tag="PK281_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48243.1"
                     /translation="MTTPGGKTISFFSGILIGVLAGLGLFFLLVNGLNPFSVFSDHSS
                     SNSQDTVVDRRNIESPKLEKTKKTTFRDENSLEIQQIDSTNSIQNQDSNILANHFDNT
                     VSADEIIVKRDELIEVRDLKLILLDKSQSKNKSDSLLKVFQGNTSNSLEYRIEFWKSP
                     INYKGFKFIRNVIVAFGLDNAEPSKLFFWDDKFYLKHGSIVYKLFITEKFESFSRVND
                     ESLLKQFR"
     gene            6023..7465
                     /locus_tag="PK281_02515"
     CDS             6023..7465
                     /locus_tag="PK281_02515"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HOY48244.1"
                     /translation="MKKFLSTLGIACVGGLAALSFSHFFQSSTNGYDRLIEERTPVQL
                     ASTHGSSLANAPDLTAAAEQSVHGVVHVKTVTSNRRAASYDPFYDFFFGNPYRNAPPQ
                     KAMASGSGVIISEDGYIVTNNHVIQNADKVEIVLNDKREYEAEVIGRDPSTDLALLKV
                     DAKNLPFIYYGNSDQVKVGEWALAVGNPFSLTSTVTAGIISAKGRNINILNEKFAIES
                     FIQTDAAVNPGNSGGALVNANGELIGINSAIASNTGSYSGYSFAVPVNIVRKVVADLL
                     EYGEVQRAFIGVVLEDLNADLGKKKDIADLKGVYVSGLNAGGAAEEGGVKEGDIILKI
                     QDVIVNNVPELQEQIGRYRPGDKVQLLINRGGTEKVLSLILKNKNGDTRLIRYAESIK
                     DELGADFAPINSNEKSSLRIQHGLRVENIREGRLRNIGIREGFIITNIGERKVDSLED
                     LTEALKSERKGLLIEGLYPNGARAYYGLGI"
     gene            7603..9063
                     /locus_tag="PK281_02520"
     CDS             7603..9063
                     /locus_tag="PK281_02520"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006256790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOY48245.1"
                     /translation="MENASATAYETELNEWIRDEKAAIELIHIIGELWFDRSIELVIF
                     RNQLVDRSASEILQLHNYALNMVGKPITIQDSLLLAKEILSAEIAPARIDLGRLATEW
                     LNEKAAYESSKDFISDKLHSFLGKDKRVLKPKDIVLYGFGRIGRIAARELIIQAGKGE
                     QLRLRAIVTRSNSDEDISKRAALLRTDSVHGPFPGTVIEDYENKALIINGHTVKMIAG
                     SNPAAIDYTAYGISNALLIDNTGSSRDREGLSEHLKAKGISKVLLTAPGKGDIPNVVH
                     GVNHASFDHQAETIFSAASCTTNAIVPVLQVIQKNLGILSGHIETIHSYTNDQNLLDN
                     FHKKYRRGRSAALNMVITETGAGSAVTKVIPDLAGKLTSNSVRVPTPDVSLAILKLTV
                     ETDTNKDDVNSVLRNAALKGDLVEQIQYSISNELVSSDLIGNPCASIVDSPATIVAND
                     KRNIVLYVWYDNEYGYTRQVIRYAKHISDVIRLTYY"
     gene            complement(9128..10096)
                     /locus_tag="PK281_02525"
     CDS             complement(9128..10096)
                     /locus_tag="PK281_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004571169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOY48246.1"
                     /translation="MQKFNSDVEAIDSLKDAYQRLDAEIGKVVIGQEDVVKDVIISIF
                     SKGHCLLVGVPGLAKTLLVTTISRVLGLDYNRIQFTPDLMPSDIVGTEILDEDRHFRF
                     IKGPLFANIILADEINRTPPKTQAALLEAMQEKAITAAGKRYELDQPFFVLATQNPIE
                     QEGTYPLPEAQLDRFMFNIWVDYPKYEDELLVVKSTTSDTKVELKSILSAEEILFYQD
                     LVRRVPVTDKVLEYAVKLAVKTRPNSEHAPEVVKKYLAWGAGPRASQFLVLGAKCHAA
                     IRGRYSPDIEDVNAVAVAILRHRIIRNYKAEAEGLSVESIIQQILN"
     gene            complement(10113..11453)
                     /locus_tag="PK281_02530"
     CDS             complement(10113..11453)
                     /locus_tag="PK281_02530"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOY48247.1"
                     /translation="MQVNRILFLILVCLNSGSLFAQSETIDKIVAVVGDKAILLSDIE
                     AQALQVEQQSGNPDPYLRCNILEDMIIQKLLLNQAEKDSVVVSDDQVDGELNKKIRYF
                     VGQIGSVEKLEAYLGKSIVQIKDDFRERIRDQLVVQQMQGKIAGDVKVSPAEVRAYYD
                     AIPKDSVPFIESEIQVAQILKKPPINQVERERVRKELQEIRQKIVDGRSFASMAAFYS
                     EDVVSAAKGGELGFVNRGDLVPEFEAVAFSLKGKEISEIVETMFGFHIIQLIERRGET
                     INVRHILISPKASASDLDVARVKLDSISQAIRLGRISFEEAALKYSDDADTKNNGGLM
                     INPNSGSTWFEVSQMDQSIFFVVDKMKIGELSDPVTVRVGEKKESYRIVSLKARTEPH
                     RANLKDDYQRIQQAAEAEKKSKLVQEWIQRKRQSFFIKIDAEFSNCHFQNTWNN"
     gene            complement(11440..12183)
                     /locus_tag="PK281_02535"
     CDS             complement(11440..12183)
                     /locus_tag="PK281_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48248.1"
                     /translation="MKELQAALPYGLSKKDSATFANDYIEQWIKKNVVLQRAENNLTD
                     NQKDVTQQLEDYRASLIIFAYERELIRQKLDTTVSNAEIEKYYQENAANFELRSNIIR
                     LKYLKLPLKTPNVDKARKWFLSSNKDDKNKLEQFANLYAVNFLLDDSNWLLLDDVIKE
                     IPMGNYSNSDFSSNRRLLELDDKEYHYLVAITGFMVKDSNAPLSFEKDNIRNIILNKR
                     KIRLIEQMQQDAYNDAMNENNIERPNAGK"
     gene            complement(12379..14415)
                     /locus_tag="PK281_02540"
     CDS             complement(12379..14415)
                     /locus_tag="PK281_02540"
                     /inference="COORDINATES: protein motif:HMM:NF025002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOY48249.1"
                     /translation="MQRNKILLATCILSGSLAFGQTAVKKKVTSTSLPAAAVPVVSST
                     PVIEDPVLLIVGGDPVTRGEFESIYRKNNSKETSSDRKALEEYLQLFINFKLKVKAAK
                     AVGKDTAQAFINELKGYRRQLATPYLTDKNVNDKLIQEAYDRMKKDVRASHILISLPA
                     DPLPKDTLAAYNKAMAIRARVLKGEDFATLASQNSEDPSAKQNGGDLGYFTSMMMVYP
                     FETSAYTTPVGEVSMPVRTKFGYHILKVTNVRDAQGQVHVAHIMVKTPENAKDSVVAA
                     NKKKMDEISERIKKGEDFGQLASQFSDDRTSGKNGGQLPWFGTGKMVPEFEKVAFDLK
                     NDNEVSEPFKTQYGWHIVKRLERKGVQSFDEVKAELKQKISKDSRSQVSRDAVILRIK
                     KESGFTEDAKALNELITKVDTSFLSAHWKSDATKAMIKPLFKLGDETTTQYDFAQFLA
                     QNQSKQPKESVVEALVRKSYDYFRNEKIIAYEDARLEQKYPEFRMLMQEYRDGILLFE
                     ITDENVWSKALKDTVGLENFHAQNKGKYMWSDRVEAVVYKSKDVATAKATRKLVEKRM
                     KKGTSIEEISTKINKSSQLNLQTEKGIFSKGDNEVIDAVSWTVGISPDKINADGSVTF
                     VEITKVVEPTPKTLQEARGMITADYQTYLEQEWIKELKAKYAVDVKQDVFNSIK"
     gene            14554..15441
                     /locus_tag="PK281_02545"
     CDS             14554..15441
                     /locus_tag="PK281_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48250.1"
                     /translation="MESKEKILDFTEKLPNREGSAEQERNNRIINSAVAYMAAFSIAY
                     VSFNVVTSLFAKRFHLFYKLFFFKVEFTQNYDLWTIESVLKTFISGPTFCLIAGVLAL
                     LIQKFYRKRPGILKTFWLWLGLHYLNFFCTELVLMPIKNTGSQGINASYLGIVADYLY
                     WEDFAKLIASILATLMLIFIGAITAKSFIQITNSTQHVFKNENRIIYLFQTVLLPYLI
                     CSGIALIYFSDGSFILNLGLVFTMFVVVVSIFINAMKNKMIMIYRLPESGMIENRFLI
                     ILASTLILMKILLNNGIMF"
     gene            complement(15448..16911)
                     /gene="guaB"
                     /locus_tag="PK281_02550"
     CDS             complement(15448..16911)
                     /gene="guaB"
                     /locus_tag="PK281_02550"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HOY48251.1"
                     /translation="MEHHSKFAEGLTYDDVLLIPDYSEVLPRETDISTHFTKSIKLNV
                     PIVSAAMDTVTESALAIAIAQEGGIGVLHKNMSIALQAAEVKKVKRSESGMIIDPVVL
                     HENAVVADALQLMKENKIGGIPVVDDNRKLVGIITNRDLRFEKRFDKPVKEVMTKENL
                     ITTTKTDLAKAEILLQDYKIEKLPVVDQDYRLVGLITYKDIIKNRLRPNACKDDFGRL
                     RVAAAVGVTGDTLERVSALVKAGADAVIIDTAHGHSKGVIEKLKEVKAAFPNLQVVVG
                     NIATAAAARMLVEAGADAVKVGIGPGSICTTRVIAGVGVPQLTAVMDVARELQGTGVP
                     VIADGGIRFSGDIAKAIAAGASTIMAGSLFAGVEESPGETIIFEGRKFKSYRGMGSIE
                     AMQTGSKDRYFQDAEDDIKKLVPEGIVGQVPFKGTLSEVLYQMIGGLRAGMGYCGAQN
                     IQVLQTKKLIRITAAGVRESHPHDVNITREAPNYSRR"
     gene            complement(17136..18419)
                     /locus_tag="PK281_02555"
     CDS             complement(17136..18419)
                     /locus_tag="PK281_02555"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOY48252.1"
                     /translation="MIQPDRLTAPATHPIDTVNPQFPEQFSLSNGMEVYSLSAGSQEV
                     LKVELLFAAGTSRYSNSLIPSFTASMLQEGTHQRNAAQIANDVDDYGAFLELDIDKDF
                     SGITLYSLNKHLAKTLPVVEDILMNSTFPEEEWEIMRSNRIQKYTVNLGKVGFVAGKR
                     FQELLYAETPYGTSFDVNDYRAMQRNELIHFYQNQYAPTAAKLFVSGKMDDSIHQLLE
                     STFGQLKGDGKFLPALALATPVTFSERKQFIPKEDAIQSAIRIGRRMFPKSNPDYFAM
                     KVLTTILGGYFGSRLMTNIREDKGYTYGIGAGVMPYRSDSYFYISTEVGSDVCSAALD
                     EIYKEIRTLRDELVSNEELDLVKNYLLGSLLKSFEGPFERMERFKNLHLYGVGNDYYQ
                     QYTAAIRAVSPHKLQELAKTWLQEDDLLELVVGKK"
     gene            complement(18416..19654)
                     /locus_tag="PK281_02560"
     CDS             complement(18416..19654)
                     /locus_tag="PK281_02560"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOY48253.1"
                     /translation="MISYTRFTLDNGLKVIVHEDPSTPLACINILYDVGARDESPEQT
                     GFAHLFEHLMFGSSINIPSYDTPLQEAGGENNAFTNNDITNYYLTLPAQNLETGFWLE
                     SDRMLSLAFSEKSLEVQRSVVIEEFKQRYLNQPYGDEWLLLRPLAYHVHPYRWATIGK
                     EIKHIEDATMEDVKAFFHRFYLPSNAIMVVAGNVKHDDVKRLAEKWFAPIPSGKRPER
                     KTIVEPEQTEPRRSTVHRDVPSDAIHKVYHMAARMDKEYHAQDLLSDVLGRGQSSRFY
                     NALVKDRQLFTDLSAHVSGSFEKGLFVISGRLRNGVKPEEAEAGIQSEISRLLNEGIS
                     EQELSKVKNKAESTHVFGEVSVLNKAMNLAFGELLGDIEMVNTEIARLQAVTVTDVMQ
                     AAKSILREENCSTLYYLRAE"
     gene            19776..20582
                     /locus_tag="PK281_02565"
     CDS             19776..20582
                     /locus_tag="PK281_02565"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HOY48254.1"
                     /translation="MQATIEQAWENRELLKDTAVQKVIRQVIEEIDKGRLRVAEPSGD
                     SWKVNDWVKKAVILYFPIQQMKTIELGPFEFHDKMQLKTGYAALGVRVVPHAVARYGS
                     FIAPGTILMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSGGVGIGGVLEPVQA
                     SPVIIEDGCFIGSRCIVVEGVHVGKEAVLGANVVITQSTKIIDVTGEKPIEYKGHVPA
                     RSVVIPGSYTKSFPAGDFQVPCALIIGQRKASTDLKTSLNDALREHSVAV"
     gene            20591..21556
                     /locus_tag="PK281_02570"
     CDS             20591..21556
                     /locus_tag="PK281_02570"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOY48255.1"
                     /translation="MKKILIIQTAFIGDVILATPILEALYTAYPDAKIDMVVRKGNQA
                     LFKGHPFLKTAFVWDKEGGKYKNLLLILIAIRRQKYDLVINCQRFAASGFLTGFSGAK
                     KTIGFDKNPFSFRFTHKIKHEIGNGLHECERNLKLLSPLGITGNKQPRLYPAALENKP
                     SQPYVCLAPASVWFTKQWPEHKWLELIETIPANLDVFLTGGPSDRDLCNRILEKSSRK
                     NLISVCGEMSLLDSAALMKGAQMNYVNDSAPMHLCSAVDAPVTAIFCSTVPSFGFGPL
                     SSISRVVETEEKLSCRPCGLHGFKACPKGHFKCAEGISAEKVLGN"
BASE COUNT         6580 a         4172 c         4113 g         6822 t
ORIGIN      
        1 acggttccaa gaaatcgttg ctccagtttt ttcaaaggtt ctaatgaggt tttgcttatc
       61 gaaaagacta taacatgcat tcaccaatgg ggaaatagga tcatctccat taacttgaga
      121 tacgaatcgt ccaacttcta aataccacca attgaatttc gtacgtttgg tcattgtcca
      181 agctttcaaa ccacctttca aacgttcatc tgataagccg tatcgtccaa caccttcaac
      241 aaaccaagaa cgattgtctt cataggattt actgaaacga atacgaggtt gaacataaac
      301 accctctgtt acattgtagg agagcatagt tatgagagga ttaataccca aatacgattt
      361 cgtgaatgaa ttgcgatgtg tatatcctcc tacaataaat tttgcgggat taaaacgatt
      421 attgacttta tcgagtgaat cgaggtaagg tttgctagtt tgaataattt cggtgctatc
      481 tgcttttcga tagttctttt cctcctctga cgtaagagtc attggacgtg tttcggtcca
      541 ataaacggtg tccttcttat tggctttttc ttcaattcta aaaatttcgt tgccaaagaa
      601 attcttcgga tatactggat gaagtttata attactaaat actccaactg atttatcgac
      661 cgccccaaag ccaaagaact tgatgtgact catcaattgc atcgacaatg gcatccaaat
      721 gctgtcctct aatgccgtat attcctgttt gatatacaaa gtgtcgacaa actgaatttg
      781 agcgtctttg gtgagaaata aatcaacgct gtgaatatgc cagctatcgt caacaataaa
      841 gatttcacct cggaagcaag gatcgttttt acgtttaggt tcgagggcta ttcgattgat
      901 cgttttacca ttatcgacaa aggttcctat cattttgaat ttgtaataaa acatggctga
      961 ttcggaaacc ggtgaaacaa agcctctttc ggaataataa tcgaattcga gcagattttc
     1021 gtagaagttc atgagcacat ctgaaacacg attccaactg aatccttgct tcattccagc
     1081 cactttagaa gcaatcattt cttctttata atcggaaggt tgacgtacat gatagcgagc
     1141 gactgattca gataaataaa ctaatcccaa atcggtacta tcaggcatat tttctttgga
     1201 aataaaaatg ggacgtttct taggaatttc atttaatcgt acgctgcctt tgatatatat
     1261 atcggatgaa aatgcatcga cttgttctag atgttctttt cgagtgcgga tggcattttt
     1321 catcacagca taggctggat ctttcgcatt tgctttgact tcaaacgttt tgatctggta
     1381 attctcgggt tcgagagcga tgcgtaaatc ctgaacatcg gtagaaatgg taattggaag
     1441 cactttcttt ttaaaaccga tggatgaagc aaccagattt tgattgtttt cctgttttaa
     1501 atccaagcta aaatttccgt tttcgttgga tgccgttccg atgcttgttc cttcgataaa
     1561 gacattcaca aagggcaatt catttccttg aatatctgta gtctttccct ttatctgcag
     1621 atattcttct ttaattccaa gcgaacgcag cacaaaagcc atttcaaatg cgcggtcttt
     1681 catagcacca tatcgaccgg aatttccacc atgtcccgct tccatgttaa ttttcaacaa
     1741 gagtaaattg gtgtcggttt ttaaactccg cattctggct acccattttg agggttccca
     1801 atatggcact tgcgagtcgt tgtatccacc cgtaaccaac atgtgaggat aagcttgcgc
     1861 gctaacattt tcataaggcg cataggattt catgtatgca taataagccg gatcgttggg
     1921 gtttccccat tcgtcatatt caaatgtggt aagcggcaaa ctcgcatcaa gcatggtgtt
     1981 aatgacatct acaaaaggca catttgcgac gacagcacca aataaatcag gtcgcatatt
     2041 gaccacagca cccatcaaca aaccacccgc acttccgcct tgtatggcta attttcccga
     2101 tttagtatat ccatctgata tgagcttttc ggcagcggaa ataaaatcgg taaatgtgtt
     2161 tttcttttta aataatttac cgttttcata ccataaacga ccacaatcat ttccaccacg
     2221 tatatgcgca acagctacaa caaaacctct ttctaataag gaaatattgg aagatgaaaa
     2281 tccaggaagt gaaggattac catacgaacc gtacgattgt aaccaagcag gattactccc
     2341 gtttttttga agtccttttt tataaatgat ggttattgga atacgagcac tgtcggcagc
     2401 aatggcataa atgcgttctg aactatattt ttcagggtcg tatccacctt ttaacgtatc
     2461 gcgacgaatg agtgtccgtt cgcctgtatt taaatcataa tcccatactt cggaaggaaa
     2521 acgcatactt gtgaatgaat agcgaacttt tgattcgtta tactcataat atccgcccaa
     2581 agacatggaa gcgggttcgg tgctagaagg caaataataa cttgaattat ctttcaaatc
     2641 aagaacagca attttctgtt gggcatcgaa ttgttccact accaggagtt ttccgccagc
     2701 cagaacagaa ttggcaatca ttacatcttt gcgatgtggc accaatactt tccaattttt
     2761 ttgacgtgaa tttgagattg gacaggtcaa aatttgaaga ttcggggctt gatcatccga
     2821 tgaaataatg aactcatttc cttcataatg ttcaaggccg tattgatgtc ctcgtctacg
     2881 tggttctact aatttaaatt gcggttgggc atcgtttaag tccatcacat agacctcgtt
     2941 tgccattgtt tgagacgtat ttagaaaaat aaatcgctct gaatcagatc ttgaaagacc
     3001 gatattgaat gtgacgtctt tttcttcaaa caagaattca tcttgaatag tattttgtcc
     3061 aagtatgtgt ttataaacac ggtaggaacg cttggtttta ggttctggaa tggtgtataa
     3121 aacggcatca tttcccatcc agcatatgcc attgatattt ttgagtgtat cagttaaaac
     3181 actccctgtt agaatgtctt tgaaaaagag gttgaaaatg cggtctcctt ttgtgtcgat
     3241 tccgtaatac attttctgat tatcgggact gataccgagc gctgatatat tgaaataatt
     3301 ataggtttta gctatttcat taaagttgat aagcgggact tctggggcat ttaaagaatc
     3361 tgcttttcga aaatatgcgg ggtaatccat acccttttca tagcgtgtga aatagaggta
     3421 tttcttcatg cgactaggca gcgattctgt ttgatcagga attctaacgc gcatttcctc
     3481 aaacattttc ttcatcaaca aagaagaacc tgccattacg cggtcgctgt agccattttc
     3541 tgcatataaa tgattaatga gttgcggttc tgttttctgg cgcatccaat gatattcatc
     3601 ttgacgaatg tcgccgtgaa tagtatcagc ccaagtgatt ttggcagcag aaggaggtat
     3661 gagtttctca gtttgagaat aaagtgaaga gatcgaaaaa agaaaaaagc acgaaatgat
     3721 caataggtaa tgtttcatca taaagaatgg aattatccta agtgcaatga gcttcgaaaa
     3781 agttacttag ataatgaaaa cgaataattc cagttaggaa ttgtttgact ttaattcaag
     3841 aatacgttgt tgtacacttt cgctagaata acgaggcatt acaaaggaac cttctacaga
     3901 tatcggcaaa taatacgctg atttctgctc gattaaatta gcaacttcta aagcttcaat
     3961 caatccaaaa atcaacgatt gtccttgttc caaaccatac gatagcttta atacgtccca
     4021 gactggtgaa aggcaggtat cgttcgattg aagtggtgga taagctaaaa atatgtcggt
     4081 aatttccttt tgctgaatag gattcgatgt aggttttagc tgaagaaata agcaaggaat
     4141 ttttctgatg cgaatcattt taagacgctg ctccatatca acaagaactt cagctccttt
     4201 ataagtaagt gaaaagtagt gtctttgaaa aataaaggct acatgtaccg gttgcttatc
     4261 agcatgaagt atcagcacat acgggtgttc agaaagcgga agtgtaagtg ccgtaatatt
     4321 ttccaccaaa aagtgagaac tcatacttca atttgcccgg taaaaacagg catagcttca
     4381 ccagtgagaa aaacatctgt aaaatcacct tcgggagatg gaatgaaaga cacttgcaat
     4441 tcaccacctc gagtaaagac cttaactggc tgaacttgaa tatgccccat gacaaaagaa
     4501 gctatagcag aagcagttac accagttcca caagataaag tttcgtcttc aactcctcgt
     4561 tcgtatgttc ttacagttaa agaatcccga tgaacttcag caaaattcac atttacacct
     4621 tcacgaagga atcggctgga attcctaatc aatcttcctt caataggaac atcaaccatt
     4681 cctgaatctt gaacaaattt tacataatga ggtgaacccg tatttaataa taaatcgctt
     4741 tcgattattt caggtaatgc tacctgttgc atttgaagtc gaacaatgta tcccgcatct
     4801 ttattcgcaa tcaattcagc tgaatgaagt ccatcgtatg cattgaacat aaatttactt
     4861 ttatcaggaa agaattgagc tgcaaatgcc gctgcgcagc gccctccgtt tccgcacatg
     4921 gaaccttcat atccatcggc gttaaagtaa atcatcctaa aatctaaacc cggctcagac
     4981 ctaaataata tcagtccatc tgcaccaatt ccgaatttcc ggtgacataa agtctgaatt
     5041 agactactgt tttgaacatt aaattttcta tctcgatcat caattaaaat gaaatcgtta
     5101 ccagtagctt gatatttttg aaattcaatc agcattatct aaactgtttt aaaagacttt
     5161 catcattcac acgggaaaat gactcaaatt tttctgtgat gaaaagttta taaactattg
     5221 aaccgtgctt gagataaaat ttatcatccc aaaagaaaag ctttgaaggt tcggcattat
     5281 ctaaaccaaa agcaacaatg acatttcgaa tgaatttaaa tcctttgtaa tttattggtg
     5341 acttccaaaa ttcaattcga tattcaagtg agtttgacgt atttccttga aaaactttta
     5401 aaagagaatc ggacttattt ttggattgac tcttatcaag taaaatgagt tttaaatctc
     5461 tgacttcaat tagttcatcc cgtttgacaa taatttcgtc tgcactaact gtattatcga
     5521 aatgattagc aagtatgttt gaatcttgat tttgaattga attggttgaa tcaatttgtt
     5581 gtatttcgag actattctca tcccgaaaag ttgttttctt agttttctcc agtttgggac
     5641 tttcaatatt tcttctgtct acaacagtat cctgagaatt agaactggaa tggtcactaa
     5701 aaactgaaaa cggatttagt ccattaacta aaaggaaaaa caaacccaaa ccagctaaaa
     5761 caccgataag aataccgcta aaaaaagaaa ttgtctttcc tccgggagtg gtcatttatt
     5821 aatccaaaaa acgttaaatc caattttcaa cacaacacca aaattctcac ttcgtttagg
     5881 gagcaaacgt aaattaattc agttggcgtt cttaaagata gataacagaa agataaacag
     5941 tgaattgttc attgcctgcc aaactatctg gaggcatgct ttttgatgtc gaattttcaa
     6001 tccaaaaact ctttattgta atatgaaaaa gtttctttca acccttggaa tagcttgtgt
     6061 ggggggcctt gcagcattga gcttcagtca ctttttccag tcctcaacta atggttatga
     6121 tcgattaatt gaggaacgca cacccgttca attagcaagt actcacggaa gtagcttagc
     6181 taatgctccc gatttgacag ctgcagcaga acaatctgtt catggtgtag ttcacgtgaa
     6241 aactgtgaca tcgaatcgaa gagctgctag ttacgatcct ttttatgatt tcttttttgg
     6301 taatccttat cgaaatgcac cgccacaaaa agcaatggca tctggatcgg gagtaatcat
     6361 ttcagaagac ggatatattg ttacgaataa ccacgtaata caaaatgcag ataaagtaga
     6421 aatagtttta aacgacaagc gagaatatga agctgaagta attggaagag atccaagtac
     6481 cgatttagca cttttaaaag tagatgctaa gaacctacct ttcatttatt atggtaattc
     6541 agatcaagta aaagtaggtg aatgggcctt agctgtagga aatccattta gtttaacatc
     6601 aacagttaca gcgggaatta taagtgctaa aggcagaaat atcaatatac tgaacgagaa
     6661 atttgctatt gaatctttta ttcaaactga cgctgcagta aatccaggaa atagtggagg
     6721 agcgctggtt aatgcaaatg gagaattaat aggaattaac tcagcaattg cctctaatac
     6781 aggctcatat tcagggtatt cttttgcagt tccagtaaat attgtgagaa aagttgttgc
     6841 agatttactg gaatatggtg aagtacagcg tgcatttatt ggagttgtat tagaagattt
     6901 gaatgctgat ttgggtaaga aaaaagatat cgctgacctg aaaggtgttt atgtatcagg
     6961 attgaatgca ggaggtgcag cagaagaagg aggagtaaaa gaaggcgata tcattttaaa
     7021 aattcaggat gtgatagtta ataatgttcc cgaattacaa gagcaaattg gcagatatag
     7081 acctggcgat aaagttcagc tgttaatcaa tcgtggagga acagagaagg ttttatcact
     7141 gattttgaaa aataaaaatg gtgatactcg cttaatccgt tatgctgaat caattaagga
     7201 tgaactcggt gccgattttg cgcccatcaa ttcaaacgag aaaagttcac ttcgaattca
     7261 acatggactt agagttgaga atattcgaga agggcgactt aggaatattg gaattcgaga
     7321 aggatttata ataaccaaca ttggagaaag aaaagttgat tcgcttgaag atttaactga
     7381 agcattaaaa tctgaacgta aaggtttgct tattgaaggt ttatacccga atggagctag
     7441 agcttattac ggtttaggta tttgaaaaat ataacaaaac aaaaaagcct gtctaataac
     7501 tagacaggct tttttgtttg atgaaatatg tattaatctg tgcttccaag gaaaaagaaa
     7561 tttctaattt cgcgacgtca aatcaataac aaaactattt ctatggagaa tgcttcagca
     7621 acggcctatg agaccgaatt aaacgagtgg attcgcgatg aaaaagcagc gattgaactc
     7681 attcatatca tcggtgaact ctggtttgat cggagtattg aattagtcat attccgaaat
     7741 caactagtag atagaagtgc aagtgagatt ctccaacttc ataactatgc cttgaacatg
     7801 gttggaaagc caatcactat tcaagattcg ctattattag ctaaagagat cctttcagca
     7861 gaaattgctc ctgcacgtat tgatttaggg agattagcta cagaatggtt gaatgaaaaa
     7921 gcagcatatg agtcttcaaa agatttcatt tctgacaagc tgcattcatt tttaggtaaa
     7981 gacaaacgtg ttttgaaacc taaagacatt gtgttatatg gatttggtcg tattggtcgt
     8041 attgctgcac gtgaacttat aattcaagca ggtaaaggag agcaattacg tttacgtgct
     8101 attgtaactc gcagtaattc agacgaagat attagcaagc gagcagcact acttcgtacc
     8161 gattctgttc atggtccttt cccagggaca gtgattgaag actatgaaaa caaagcatta
     8221 atcatcaatg gccatactgt taaaatgatt gcaggaagta atccggcagc cattgattat
     8281 acagcttatg gaatttcaaa tgcattatta attgacaata ctggttcatc cagagatcgt
     8341 gaaggattaa gcgagcattt gaaggcaaaa ggaattagca aagttctact tacagctcca
     8401 ggaaaaggtg atattccgaa tgtagttcat ggagttaatc atgcatcctt tgatcatcaa
     8461 gcagaaacta ttttctcggc agcatcttgt acaaccaatg cgatagttcc agttttacag
     8521 gttattcaaa aaaatctcgg gattttaagt ggtcatattg aaacgattca ctcgtataca
     8581 aacgatcaga atcttttaga taatttccat aagaagtatc gtcgtggccg ttctgctgca
     8641 ttgaatatgg ttattacaga aaccggagct ggaagtgcag taacgaaggt tattcctgac
     8701 ttagctggaa aattaacttc taattcggtt cgtgtaccaa caccagatgt ttctttagca
     8761 attctcaaat tgactgttga aacagatacc aataaggatg atgtaaacag tgttttacga
     8821 aatgccgcac ttaagggtga tttagtagaa cagattcaat attctatttc aaacgaactt
     8881 gtatcgagtg atttgattgg taatccttgt gcgtcaattg ttgacagtcc tgcaaccatt
     8941 gtagcaaatg ataaaaggaa tatcgtattg tatgtttggt atgacaacga atatggttat
     9001 acaagacaag taattcgtta tgcaaaacac atttcagatg ttattcgctt aacgtattat
     9061 tgatcctaat tcaattcata aaaaaagccg gaaataaatt tccggctttt tttatgatgg
     9121 actatttcta gtttagtatt tgttgaatga tggactctac acttaagcct tcagcttcag
     9181 ccttataatt tctgataata cgatggcgaa gaatagcaac ggctacagca ttaacatctt
     9241 caatgtcggg agaatatcga cctctaatag ctgcatgaca tttagctccc aaaacaagga
     9301 attgagaagc acgtggacca gcaccccaag caagatattt tttcactact tcgggagcat
     9361 gttctgagtt aggacgtgtc ttaacagcca attttactgc atattccaat actttatctg
     9421 ttacaggaac tcgtcttact aagtcttgat aaaacagaat ttcttcggca gaaagaatgg
     9481 atttaagttc aactttcgtg tcagaagtgg tgcttttcac caccaataat tcgtcttcat
     9541 acttaggata atcgacccaa atattgaaca taaaacggtc aagttgcgct tctggaagtg
     9601 gatatgtacc ttcttgttca attggatttt gagtagctaa aacaaagaaa ggttgatcta
     9661 attcgtatcg tttacctgca gcagtgatcg ctttctcctg cattgcttct aagagcgcag
     9721 cttgagtttt aggaggagtt cggttaatct catcggccaa aataatgttg gcaaaaagag
     9781 gccctttgat aaaacgaaaa tgtctgtctt cgtctagaat ctcagtacca acgatatcag
     9841 aaggcatcaa atcgggagtg aattgaatcc ggttataatc aagcccaaga actctagaga
     9901 ttgttgtaac aagcaatgtt ttagctagtc cgggtacacc aacgagtaaa caatgtcctt
     9961 tgctgaatat ggaaatgata acgtctttaa caacatcctc ttggccaatt accactttcc
    10021 cgatctcagc atccaggcgt tggtatgcgt ctttgagact atcaattgct tctacgtcgg
    10081 aattgaattt ttgcatgaaa agaattaatt agttagttgt tccaagtgtt ctggaaatgg
    10141 caatttgaga attcagcatc aattttaatg aaaaatgatt gacgttttct ttgaatccat
    10201 tcttgaacaa gcttcgattt tttctccgct tcagcagctt gttgaattcg ttgataatcg
    10261 tctttcagat ttgcacgatg aggttctgta cgcgctttaa gtgaaactat acgatacgat
    10321 tctttctttt caccaactcg aacggttaca ggatcagaaa gttctccgat cttcatttta
    10381 tctaccacaa agaagatgct ttgatccatt tgagaaactt caaaccaggt tgaacctgag
    10441 tttggattaa tcataagacc tccattattt ttggtatcag catcatctga atactttaaa
    10501 gctgcttcct caaatgaaat tctacctaaa cgaatcgctt gagaaatact atccaactta
    10561 acacgagcaa catctagatc agaagcggat gctttgggag agattagaat gtgtcgaaca
    10621 tttatggtct cccctcttcg ttcaattaat tgtatgatat gaaatccgaa catggtttca
    10681 acaatctccg atatctcctt tccttttaat gaaaaagcta cagcttcgaa ttcaggaaca
    10741 agatcacctc ggttaacgaa acctaattca cctcctttag ctgcagaaac aacatcttca
    10801 gaataaaaag cagccattga agcgaaagaa cgaccatcaa cgattttttg tcgaatctct
    10861 tgtaattcct tgcgaacacg ctctctttca acttgattga ttggtggttt ctttaagatt
    10921 tgagctacct gaatttcact ttcgatgaac ggaacgctgt ctttaggaat tgcatcgtaa
    10981 taggctctta cttcagcggg tgacactttc acatcgcctg caattttacc ttgcatttgc
    11041 tgcacaacta attgatcgcg aatacgctct ctgaaatcat ccttaatctg gactattgat
    11101 tttcctaagt atgcttctaa cttttcgact gatccaattt gaccaacgaa ataccgtatc
    11161 ttcttattca attctccatc tacttgatcg tctgaaacaa caacactatc cttttcagct
    11221 tgattaagta gtagtttctg aatgatcata tcttcgagaa tgttgcaacg aaggtatggg
    11281 tcaggatttc ccgattgttg ctctacttgc aaggcttgag cttcaatatc agaaaggagt
    11341 attgctttgt cgccaacaac agcaacaatt ttatcgattg tttcagattg agcgaacaga
    11401 ctgcccgaat ttaaacacac gaggataaga aagaggattc tatttacctg cattcggacg
    11461 ttcaatatta ttttcattca tggcatcgtt ataggcatct tgctgcattt gttcaattag
    11521 cctgattttg cgtttattaa gaataatatt tcggatatta tccttttcaa aactaagagg
    11581 agcattactg tctttcacca taaaaccggt aatggcaaca aggtagtggt attccttatc
    11641 atctagttct aataaacgtc tgttgctaga aaaatctgaa ttggaataat ttcccattgg
    11701 aatttccttg attacatcgt ctaataataa ccagttagaa tcatctagca ggaaattaac
    11761 ggcatagaga tttgcaaatt gctccagttt atttttatcg tctttgtttg atgaaagaaa
    11821 ccattttctg gccttatcaa cgttaggagt tttaagaggt agtttgaggt attttaaacg
    11881 tataatgtta cttctcaatt caaaatttgc tgcattttcc tgataatact tttcaatttc
    11941 agcattagat accgttgtat caagcttttg gcgtattaat tctcgttcat aagcgaaaat
    12001 tattaatgaa gctcgataat cttctaattg ctgagtaaca tccttttggt tatcggttaa
    12061 attattttcg gcacgttgaa gaacaacgtt tttcttaatc cattgttcga tgtaatcgtt
    12121 tgcaaaagtt gcactatctt ttttggaaag cccataaggc aaagcggctt gcagttcttt
    12181 caagcttaag tacttatcat taactcgtgc aaccatgtta tcctccttat tatctcctcc
    12241 gcaggaagag atagtaaaca gaacgaaaca catgcaaatt aatctaagca gaatcatgaa
    12301 agtgtgtatg cgtcgggaga aaatcaaacg gcccgttcag gttaatctac caaaacgggc
    12361 cgaaatgaaa tcaattaatt atttaatgga attaaaaaca tcctgcttaa catcaacagc
    12421 gtatttagct ttcaattctt ttatccattc ttgttctagg tatgtttgat aatcggctgt
    12481 aatcatacca cgagcttctt gaagcgtttt aggagttggc tcaacaactt tagtaatctc
    12541 aacaaaagtt acagaaccat ccgcattgat cttatcaggt gatattccaa cagtccaact
    12601 aactgcatcg ataacttcat tgtcgccttt agagaaaata cctttttcgg tttggagatt
    12661 caattgagaa gacttgttaa tcttcgtaga aatctcttcg atacttgtac cttttttcat
    12721 gcgtttttct accagtttac gagttgcttt agcagtagct acatctttag atttgtaaac
    12781 tacagcttca actcgatcgc tccacatgta tttcccctta ttttgagcat gaaaattctc
    12841 aagtcctact gtgtctttaa gagcttttga ccaaacattt tcgtcggtaa tttcgaataa
    12901 caaaattcca tcccggtatt cttgcattaa catgcggaac tcaggatatt tttgttctaa
    12961 acgggcatct tcgtatgcaa tgattttttc atttctgaag taatcataag atttacgaac
    13021 taaagcttca actacacttt ctttaggttg tttgctttga ttttgagcta agaattgcgc
    13081 aaaatcgtat tgagttgttg tttcatcccc caatttgaac agtggtttga tcatcgcttt
    13141 agtagcatct gatttccaat gagcacttaa gaagctagta tcgaccttag tgattagttc
    13201 attaagagct ttagcatctt ctgtaaaacc actttccttt ttaattcgaa gaattacagc
    13261 atcacgactc acttgagagc gagagtcttt gctgatcttt tgtttcaatt cagcttttac
    13321 ttcatcgaac gattgaactc cttttctttc caatcgttta acgatatgcc aaccatattg
    13381 agttttaaaa ggttcgctta cttcattatc attttttaaa tcgaaagcta ctttctcaaa
    13441 ttcaggaacc attttaccag taccaaacca aggtagttgt ccaccatttt ttcctgaagt
    13501 tctgtcatca gaaaactgag aggctaattg tccgaaatct tctccttttt taattcgttc
    13561 ggagatttca tccattttct ttttgtttgc tgcaactaca gaatctttag cattctcagg
    13621 ggttttcacc atgatgtgag caacatgaac ttgaccttga gcatcacgaa catttgtcac
    13681 tttaagaatg tggtaaccaa attttgtacg aactggcatt gatacttctc ctacaggagt
    13741 agtataagct gatgtttcaa aaggataaac catcatcatg gaagtaaaat atcctaaatc
    13801 accaccattt tgtttggctg aaggatcttc tgaattttgg ctagcaagcg ttgcgaaatc
    13861 ctctccttta agaacacgtg cacgaattgc cattgctttg ttataggcgg ctaacgtatc
    13921 cttaggaagt gggtcagcag gaagactaat aaggatatga cttgctctga catctttttt
    13981 catgcgatcg tatgcttctt gaataagctt atcgttcaca tttttatctg tcaaataagg
    14041 agttgctaat tggcgtcgat agccttttaa ttcattaata aaagcttgcg cggtgtcttt
    14101 acctactgct tttgcagctt ttacttttaa tttaaagttt ataaataact gaagatattc
    14161 ttccagagct ttccggtcgg agcttgtttc tttcgagtta ttttttctgt agatactttc
    14221 aaattctcct cgtgtaacgg gatccccacc aacaattagt aaaacaggat cttcaataac
    14281 aggagttgaa ctaacaaccg gaacagcagc tgccggtaaa gaggtagaag taaccttttt
    14341 tttcacggca gtttgtccaa aagccagtga acctgaaagg atacaggttg ccaataaaat
    14401 cttgtttctc tgcattttat ttgttcttac gtaatagagc ctgcgaaaat agtcgaatac
    14461 tcagataaat caaaggatac aatcttatat agacaggtgt agtaatttag gatgatttta
    14521 agatatattt gcacgaaaca ggccctctat atcgtggaat ctaaggagaa aatacttgat
    14581 tttacagaaa aactacccaa ccgggaaggt tctgctgaac aggaaaggaa taaccgaatc
    14641 attaattcgg ctgttgccta tatggctgca ttctccattg catacgtgag tttcaacgtt
    14701 gttacttcac tttttgcgaa gcgattccac ctcttctaca aactgttttt cttcaaagtt
    14761 gaattcacac agaattacga tttgtggaca attgagtcgg tattgaagac ttttatttct
    14821 ggtcctactt tttgcttgat agcaggagtt ctagctttac taattcaaaa gttttatcga
    14881 aaacgccccg gtattttaaa aacattttgg ttgtggcttg gtcttcacta cctgaacttt
    14941 ttctgtaccg aattggttct aatgcctatt aagaatactg gttcgcaagg aattaatgct
    15001 agttatttag gaattgtagc tgattacctt tattgggaag atttcgccaa gcttattgcg
    15061 tcaattttag caactctaat gctcattttc attggagcaa taaccgctaa atcatttatt
    15121 cagatcacta atagtactca acacgttttc aaaaacgaaa acagaattat ttatttattc
    15181 caaaccgtat tattacctta tttaatttgc tctggaatag cattaatcta tttctccgat
    15241 ggttccttta ttttgaattt aggattagtc ttcaccatgt ttgtggtagt ggtatcgatt
    15301 ttcattaatg ccatgaagaa taagatgatc atgatttaca ggttgcccga atcaggtatg
    15361 attgagaacc gatttctgat catattagca tcaaccctta ttctgatgaa gattcttctg
    15421 aataacggaa ttatgttcta ggcggattta tcgccttgaa taattcggag cttcacgcgt
    15481 aatatttaca tcatgtggat gcgattctcg cacacctgct gctgtgatac gaatgagttt
    15541 cttagtttgt aagacttgaa tgttttgtgc accgcagtat cccattccag ctcttaatcc
    15601 accaatcatt tgatataata cttccgacaa tgttccttta aaaggcactt gcccaacgat
    15661 accttctgga actagtttct taatatcatc ctcggcatct tgaaaatatc ggtctttgga
    15721 acctgtttgc atagcttcaa ttgaacccat gcctcggtaa gacttgaatt ttcgtccttc
    15781 gaaaatgatt gtttctcctg gagattcttc tacaccagcg aataaagaac cagccatgat
    15841 ggtagatgct ccggcagcaa ttgctttagc aatatcaccc gaaaaacgaa ttccgccatc
    15901 agcaataaca ggaacacccg tgccttgaag ctcacgtgct acatccataa cagcagttaa
    15961 ttgaggaact ccaacacctg ctatcacacg agtagtgcaa atagaacctg gtccaattcc
    16021 aacttttacc gcatcagcac ctgcttcaac caacattcta gcggcagcgg cagttgcgat
    16081 attacctact acaacttgaa gattaggaaa agcggccttt acttctttca atttttcgat
    16141 aacacctttg gaatgcccgt gtgccgtatc gataataaca gcatctgcac cagctttgac
    16201 aagggcagaa actcgctcta atgtatcgcc tgtaactcct actgctgcgg caactcgtaa
    16261 tcgaccaaaa tcatctttac atgcgttagg tcgaagtcgg tttttaataa tgtctttgta
    16321 ggtaattaat ccgaccaatc gataatcctg atcaacaacg ggtagttttt caattttata
    16381 atcctgtaaa aggatttcag cttttgctaa atcggtttta gtcgtagtga tgaggttttc
    16441 tttggtcatc acttctttca ctggtttatc gaaacgtttt tcgaaccgaa gatctcggtt
    16501 ggtgataatt ccgactagtt ttcgattatc atcgacaact ggaataccac cgattttatt
    16561 ttccttcatt aactgaagtg catctgcaac aacggcattt tcatgtaaaa caactggatc
    16621 gataatcatc ccactttcgg aacgcttcac ctttttcact tcagcagcct gtagagcaat
    16681 gctcatgttt ttatgcaaaa cgcctattcc tccttcctga gctatagcaa tcgctaaagc
    16741 tgattcggta acagtatcca tagcagcaga aacgattgga acgtttagct ttatggattt
    16801 agtaaaatga gtgctgatat cagtttctcg aggaagtact tccgaataat ctggaattaa
    16861 aagtacgtcg tcgtatgtta aaccttccgc gaattttgaa tggtgttcca tggcaaccgg
    16921 gtgaacgggc ttagtcccga attgcgcgca aatgtaggaa ttttaactgc ctgaattatt
    16981 ccgaaatatt aaattaataa tacacttccg tcaattctat ttcttgccaa acttcaagag
    17041 tgctataaaa gaaacgctgc aacctaacgc ttacatcctt catttgaatg atggtaagca
    17101 atcccggcag cagcgttttg tgataaacta atagattatt tttttccgac aaccaattca
    17161 agtaaatcgt cttcttgtaa ccacgtttta gcgagttcct gcaacttatg aggactcaca
    17221 gcacgaatgg cggcggtata ttgctggtaa taatcgttgc caacaccata caaatgaaga
    17281 ttcttgaatc gctccatgcg ttcaaacggt ccttcaaaac ttttcaataa agagcctaag
    17341 agataattct tcaccaaatc gagctcttca ttgcttacca attcatcacg aagcgtgcgt
    17401 atttccttat aaatctcatc gagtgcagct gaacaaacat cagatccaac ttcggtgctg
    17461 atatagaaat agctatccga acgatagggc atcacacctg caccaattcc gtatgtatag
    17521 cctttgtctt ctcggatatt ggtcattaat cgtgaaccaa aatagccacc taatatggtt
    17581 gttagcactt tcatggcgaa ataatccgga ttgctttttg gaaacattcg tcttccgata
    17641 cgaatagcag attgaattgc atcttctttt ggaatgaatt gtttccgttc agaaaatgtt
    17701 acgggagtag cgagtgctaa tgccggcaag aatttaccgt caccttttaa ttgaccaaac
    17761 gtggattcca ataattgatg aatggaatca tccatttttc cactcacaaa caatttagct
    17821 gcagttggag catattgatt ttgatagaaa tggattaact catttcgttg catagcgcga
    17881 taatcattca catcgaatga agttccataa ggagtttctg cataaagcag ttcttgaaaa
    17941 cgcttacccg ctacaaatcc aactttgcca agattaaccg tgtatttctg aattcggttg
    18001 gagcgcataa tttcccattc ttcttcgggg aaggtggaat tcatcaagat atcttccact
    18061 acaggaagtg ttttcgctaa gtgcttattg agcgaataca atgtgatacc tgagaaatct
    18121 ttatcaatat cgagttctag aaaagcacca taatcatcca catcattggc aatttgtgca
    18181 gcattccttt ggtgtgttcc ttcctgaagc atagatgctg taaaagaggg aattaaggaa
    18241 ttgctataac gtgatgtacc cgcagcaaat agcaattcaa ctttgagcac ttcttgagaa
    18301 cccgccgaca aactatacac ttccattcca ttggaaagcg agaattgttc agggaactgc
    18361 ggatttaccg tatctattgg atgtgtggca ggagctgtta aacgatcagg ctgtatcatt
    18421 cggcacggag ataataaagg gtagaacaat tttcttcacg caaaatgctc ttggcagctt
    18481 gcatcacatc agtaacagta accgcttgca aacgtgcaat ctcggtattc accatttcaa
    18541 tatcacccaa caattcgcca aatgctaaat tcatggcctt attgagcaca ctcacttccc
    18601 caaaaacatg ggtactttct gctttatttt tcactttgct taattcctgc tccgagattc
    18661 cttcattcag caatcgtgaa atctccgatt gaattccagc ttctgcctct tctggtttca
    18721 ctccatttcg aagtcgccca gaaatcacaa acaagccttt ttcaaaactt ccacttacat
    18781 gagccgacaa atcggtaaac agctgacggt ctttcaccaa agcattgtag aaacgggaag
    18841 attgaccacg tcccagcaca tccgaaagta aatcttgagc atggtattct ttgtccatac
    18901 gtgccgccat gtgatatact ttatgaatcg catccgaagg cacatcgcga tgaacagtgc
    18961 ttcgacgagg ttcagtttgt tcaggctcaa cgatggtttt acgttcaggt cttttgccag
    19021 aaggaattgg agcaaaccac ttttcagcta gacgtttcac atcatcatgc ttaacatttc
    19081 cagctactac cataatggca ttagatggca aatagaaacg atgaaaaaac gccttcacat
    19141 cttccatggt agcatcttca atatgcttta tttccttccc aattgtagcc caacgataag
    19201 gatgcacgtg ataagctaag gggcgtaaaa gcaaccattc atccccataa ggctgattta
    19261 aataacgttg cttgaattct tcaatcacca cactacgttg cacttccaaa cttttttctg
    19321 aaaaggctag actgagcata cgatcggatt ctaaccaaaa tccagtttct aagttttgag
    19381 ctggaagggt gaggtaataa ttggtgatgt cattattggt gaatgcattg ttttcaccac
    19441 cggcttcctg caaaggtgtg tcgtagcttg gaatgttgat cgaactacca aacatgagat
    19501 gctcaaataa atgagcaaat ccggtttgtt caggagattc atcccttgca cctacatcgt
    19561 agaggatatt tatgcaagcc aatggcgtgg aaggatcttc atgaacaatg acttttagcc
    19621 cattgtcgag cgtaaaacga gtatatgaaa tcatggggcg aatatactgc ttgttactag
    19681 tgatgctgga gttagaaaaa attgattcca agatttattc aatctactaa ccattaactg
    19741 tgaaagaagg ttcaatggat tatctttggg gcattatgca agcaactata gaacaagcct
    19801 gggaaaacag ggaattactg aaagacactg ctgtacagaa agtcatccgt caagtaattg
    19861 aagaaataga caaaggtcga ttacgtgtgg ctgaaccttc aggagattct tggaaagtaa
    19921 acgattgggt aaaaaaagcg gtaatcctgt atttccctat ccagcaaatg aaaaccatag
    19981 aactcggacc attcgagttt cacgataaaa tgcaacttaa gactggctat gctgctttag
    20041 gagttcgtgt agttccgcat gcagttgctc gttacggaag cttcatcgct ccaggaacga
    20101 ttttaatgcc ctcgtatgtg aatattggag catatgtaga tgaaggaact atggttgata
    20161 cttgggctac tgttggttca tgtgctcaaa ttgggaagaa tgttcacttg agtggtggag
    20221 ttggaattgg aggagtatta gaaccagttc aggctagtcc agttatcatt gaagatggct
    20281 gttttattgg ctcgcgctgt attgttgtag aaggcgttca tgttgggaaa gaagctgttt
    20341 taggtgcgaa tgtagttatc acgcaaagca ctaaaattat tgacgttacc ggtgagaaac
    20401 caattgaata taagggacat gttcctgcga gatctgtggt tattccgggc tcttatacta
    20461 agtcatttcc tgcaggcgat tttcaagttc cttgtgcctt aattattgga caacgaaaag
    20521 catcaaccga tttaaaaaca tcgctcaatg atgctcttcg cgagcatagt gttgctgttt
    20581 aagagaagct ttgaaaaaga tcctgattat tcaaacggca tttattggtg atgtgatttt
    20641 agccacaccc attttggaag cattatacac tgcttatcca gatgcgaaga ttgacatggt
    20701 ggttaggaaa ggaaatcaag ccttatttaa aggccatcct tttttaaaaa ctgcctttgt
    20761 ttgggataaa gaaggaggga aatacaagaa tttattgttg attttaattg ctattcgtcg
    20821 gcagaaatac gacttagtaa ttaattgtca acgctttgct gcatctggat ttctaactgg
    20881 tttttcggga gctaaaaaga caattggttt cgataaaaat cctttttcgt ttcgatttac
    20941 tcataaaatc aaacatgaaa ttgggaatgg attgcatgaa tgtgagcgta atctcaaact
    21001 tctcagtccc ttaggaataa ctggaaataa acaaccccga ttgtatcctg ccgccttaga
    21061 aaacaaacct agtcagccgt atgtgtgttt agcaccagca tcggtatggt ttacgaaaca
    21121 gtggcctgaa cataaatggc tagagttgat tgaaactata ccagcaaacc tggacgtttt
    21181 tttaacgggc ggtccatctg atcgggattt atgcaatcga atacttgaaa aaagttcaag
    21241 aaaaaatctg atttctgttt gtggggagat gagtttactg gattcggcag ccttaatgaa
    21301 aggtgctcaa atgaactatg tgaacgattc tgcaccgatg catttatgtt ccgcggtaga
    21361 tgcgccagta acagcaatat tctgtagcac agtgccttca tttggctttg gtcctttgtc
    21421 aagcatttcg cgggttgttg aaacggagga gaaattatcc tgtcggccat gtggattaca
    21481 tggatttaaa gcctgtccga aaggtcactt taaatgtgcc gaaggaatat ctgctgaaaa
    21541 agtgcttggt aactgagcag taattactgg ataaaaaacc taatttgcgc ccgttttggc
    21601 gacaataaac cttaccggac ctgtatttga gcttctccga aaatcggttt cagaactcga
    21661 tgtgcgagct tttgttattg gtggata
//
LOCUS       DAOOQW010000021        21567 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_2975_length_21567_cov_6.246374, whole genome shotgun sequence.
ACCESSION   DAOOQW010000021 DAOOQW010000000
VERSION     DAOOQW010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 21567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21567
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2975_length_21567_cov_6.246374"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            286..1080
                     /locus_tag="PK281_02575"
     CDS             286..1080
                     /locus_tag="PK281_02575"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011708212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HOY48256.1"
                     /translation="MQQYQALLAHILENGTQKHDRTGTGTISCFGYQMRFDLSKGFPI
                     VTTKKVHLRSIIHELLWFLKGDTNIAYLKENGVSIWDEWADENGNLGPVYGHQWRSWP
                     GKNGETIDQISKLVHMLRTNPDSRRLIVSAWNVADVDQMALPPCHCLFQFYVADGKLS
                     CQLYQRSADTFLGVPFNIASYALLTLMLAQVCGLEPGEFVHTFGDVHLYSNHLEQARL
                     QLSRDPKPLPVMKINPAVKELFDFTFDDFTLEGYEPHPAIKAAVAI"
     gene            1077..1565
                     /locus_tag="PK281_02580"
     CDS             1077..1565
                     /locus_tag="PK281_02580"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002687527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HOY48257.1"
                     /translation="MILSIVVATGKNGEIGKDNRLLWHLPADLKYFKNLTTGHPIIMG
                     RHTFESIGRALPNRRNIIITRQLDYQAVGVDIVHSLEEAIALVKDQHEVMVIGGADVY
                     QQALPKANRIYITRVDIEPEADRFFPEIDMSEWNVHVDVGYPADEKNAYSLTFQMLER
                     KN"
     gene            1567..3516
                     /locus_tag="PK281_02585"
     CDS             1567..3516
                     /locus_tag="PK281_02585"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HOY48258.1"
                     /translation="MNFSAADRKILANVVSTFVEIPNQATWIDSVGIAEKIAALIAEK
                     SPEEQADFRQLLDLLESPFLGFTWGGAFRSFENLSAEERQSLFRSWSASRFALLRKGY
                     QALKKLSTFIAYSLCNESGQSFSQPITQYQAHQETSITEQQAIPLIKPGIGIRCEVLI
                     VGSGAGGGVAAARYAQAGKDVLIVEKGPWMPEQEMTNLEAEMVSQLYEERGALSTVDG
                     AMTVFAGACLGGGTTINWQACIKTPDFVLEEWAEESGIADFVKPVFQYHLDSVWKRIG
                     ADREEVIHNTNNQLLIKGSNLLGQEVNPIAKNSSHCNSDGAHRCGWCGFGCKQGSKQS
                     TLKTYIQDAIELGTRILDRTYIERLLFSGNQVIGAEGFHTDPLGNKTEVRIHCERVIV
                     SAGSIHSPALLMRSGIRHPELGKNLYLHPVVPVPAIYSEEVKAWSGAMMTSTNDEHIR
                     LNGRYGYKIETPPPHPGLLALGMPWKDAQSFQERMAQISHLGVFIVLSRDEFSGAIRL
                     SKDGRPMIDYSLHANDKARLIHGMKNAARIHAAAGAKEIALPHIQPTFVDASVVNDTV
                     RFEKLIDEMRWDKGDYTLFSAHQMGTCRMGTSYTHDVVSPEGKVWGVNGVYVVDASVF
                     PKSSGANPMVSILAISDYLCCQQLG"
     gene            complement(3517..4251)
                     /locus_tag="PK281_02590"
     CDS             complement(3517..4251)
                     /locus_tag="PK281_02590"
                     /inference="COORDINATES: protein motif:HMM:NF040269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOY48259.1"
                     /translation="MEKLTSVRFAPSDDLNFYFRAAFSVSCVIFGLHEKKIKVLLVKR
                     KRDPFKNTFSLPAELVYPNDSLEEHVDQIVQRVTGCERFYKKQIRAFADTSRHPLGRV
                     VSIGYYCFIDWHQCRQFEPNEDHNAAEWFDLKSLPVLAFDHKEIIEAANRRLLAKMST
                     QLTGFNLLPEKFTLKQVQEMYEAILGHELDKRNFRRKILSQDVILETGEQLSPWEESG
                     KAPMLFQLDEKRYQQLKVEGNKFEIF"
     gene            complement(4429..4998)
                     /locus_tag="PK281_02595"
     CDS             complement(4429..4998)
                     /locus_tag="PK281_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017842987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HOY48260.1"
                     /translation="MLFNLDEIITITVTLFAIIDVVGAVPVLISMKSRLGEIESGKAT
                     LASGLLMILFLIFGTRLLGVLGVDINSFAVAGSIVIFIMGLEMVLGIELFRQDPDVKS
                     GSIIPIAFPIIAGSGTLTTVMSLRANFSVYNILIGILINLIAVYLVLRSINYLERKLG
                     KSGLFVIRKFFGVILLAISIKIFKANIGL"
     gene            complement(5044..6060)
                     /locus_tag="PK281_02600"
     CDS             complement(5044..6060)
                     /locus_tag="PK281_02600"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013552479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOY48261.1"
                     /translation="MKTAVVILNWNGKHLLEQFLPKVIACSPNAEVVVADNASSDDSV
                     AMMRRTFPSVTIIENPSNNGYAGGYNEALAQVDADYYVLLNSDIEVTPNWIEPVIKHF
                     ESDPLVAAIQPKIRAFKQRNQFEYAGAAGGYLDRLGFPFCRGRIFEECEVDNGQYDDV
                     KEVFWASGACMFVRASVFHECEGFDELFFAHMEEIDLCWRMKNRGYKILVEPASVVFH
                     IGGGTLSKSNWRKTYLNFRNNLELIYKNIEGKYLFRSLFMRMTLDGIAAFKFLFTNGF
                     SHFYAIIRAHLHFYRKLPSIRQKRRKLKAVEHNRNSTGVYMGSIVADYFIRGKKTFRD
                     LDIH"
     gene            complement(6057..6920)
                     /locus_tag="PK281_02605"
     CDS             complement(6057..6920)
                     /locus_tag="PK281_02605"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOY48262.1"
                     /translation="MNFFYYLLRGGGFLIAQIPLFLLYPFSGFLAWIASDLVRYRRDV
                     VLANLKASFPEKSDRERLKIAKGFYLNFTDIMVESFKMLSLNPRLIPSRVKVLNPELL
                     DSYKQQGRSIIAVSGHYCNWEWMGVGLKSVTGMRALVTYKPLSSKLMDRLMKEIRQLN
                     GSELISMQQTFRSIATSKEPVISLLVADQAPHPENAYWTIFLNQDTPVFLGAERIAKS
                     TGHVVVFLAMRRVKRGHYTLEFQELCLDPKAQPEFAITEMHLRALESLIIESPQNWLW
                     SHKRWKHKRPA"
     gene            complement(6917..8389)
                     /locus_tag="PK281_02610"
     CDS             complement(6917..8389)
                     /locus_tag="PK281_02610"
                     /inference="COORDINATES: protein motif:HMM:NF040439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HOY48263.1"
                     /translation="MRQFLLFISFLLFSVGTFAASNGNSVQAIFSHASFMMNGRKPYV
                     ETYIMVRGSSVVFRKTDSGKYQGTIEVILTVSQDGQIRYADKYNLLSPETDDSVHITF
                     NFIDQQRIPLGEGNYELGLRIRDVNGIGEPVEATEPLEVKFPNNAVSFSDIQLVESYT
                     KTEMPGSLSKGGYDLIPHINDVFVKDETKLIFYSEIYQTLTFLGEGKKYLCNFFLRNS
                     NTNEQLTSYSGFTRYTASEVSVLMKEFDITNLPTGQYNLELEVRDAENQVLATKKVSF
                     VRSNPGEIIAPDNYTSTSLLNSFVLNYTNPDSLAEHIRCLYPISYELEKNIATNVIKA
                     KDLQQMQQYMLSFWINRDALHPQEAWEKYFFEVRKVVKEFSTSIRKGYNTDRGRVYLQ
                     YGPPDNRTQMPHEPSAYPYEIWQYYKVKGQSNRRFIFYNPDLVTNDFKLIHSDALGEI
                     MNDQWQLLIYKRDTQTNDIDQTTPSGRFGSQIQQNYQTPR"
     gene            complement(8444..9946)
                     /gene="glpK"
                     /locus_tag="PK281_02615"
     CDS             complement(8444..9946)
                     /gene="glpK"
                     /locus_tag="PK281_02615"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HOY48264.1"
                     /translation="MKKILAIDQGTTSSRCIIFNEQGQVIATAQEEFPQYFPHAGWVE
                     HNPLEILQSVKNSYQQVLLNAGISCDQITCIGITNQRETTIIWDRKTGQPIYPAIVWQ
                     SRQSLEICERINARGISQAIQDKTGLVVDAYFSASKIAWILEQVPNARQRAEKGELAF
                     GTVDTWLLWNLTGGKVHATDYSNASRTLLFNIHECCWDKEILQWFDIPESILPEVKDS
                     SGYFGTTVDGYFDAGIPIHGIAGDQQAALFGQGCIQPTMAKNTYGTGCFMLMNTGNVP
                     VKSSCGLLTTIAWGLNGQISYAVEGSVFVAGSAIQWLRDGLGLFRDSSEIEELATQVE
                     DTNGVYFVPAFVGLGAPHWDSEARGMIVGLTRGTQKSHIIRAALEAMAYQTSDVFEAI
                     QQDAGMKISVLFVDGGASANNLMMQFQSDLLQVDVIRPQNTETTALGAALLAGLSAGI
                     WTSAQACSEVAQQGKVFKPNSERQRITQLYAGWKLAVQATIGFKPRPMSL"
     gene            complement(10018..10539)
                     /locus_tag="PK281_02620"
     CDS             complement(10018..10539)
                     /locus_tag="PK281_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013307689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1572 family protein"
                     /protein_id="HOY48265.1"
                     /translation="MNTSHYLQSVISIFNQYKTTAEKAMVQLSDEELFWCANEESNSI
                     AIIVNHLAGNMRSRWTDFLHSDGEKSWRNRELEFEPITTNRTDLLANWESGWDCLFFA
                     LDQVNEDNFNTIIFIRNEPHSITEAINRQVAHYTFHIGQIVWIAKMLKNSAWVSLSIP
                     KGGSSDFNKAKGI"
     gene            complement(11015..12967)
                     /locus_tag="PK281_02625"
     CDS             complement(11015..12967)
                     /locus_tag="PK281_02625"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOY48266.1"
                     /translation="MIQLRLFFFSIFLFLILSHVNGQDSVIRDLENQVRNAADDTTRL
                     RLYSELTWEYLGVSLEKSDEYAQLELKLAESIHSDFYIASAYNDIGIVLIRKNRFRES
                     LKTNKKALEIRQRINDKPGIASSYSKIGHCYTEMNQLSEAHDAQLKALALFRELNDQV
                     KEAYTLNNLCVIYMNLQQYDKLETYANQSYRVLSRLGDKLGIANSLNYLATVEEYKGD
                     FNKALQLELKSVQIREEERDSVNLEGAFNNVGIYYRKLGKTQEGLKYYLKALEVAQKL
                     ENPISIALYLCNIGNVYLDLKSYSKAEEYLLQAQEITLRDSVSANRSALFKSLADLYL
                     LTNRKELALKYYDKSSLVKDSLFSESMAEKFTEMETRFETERTEQENALLLKENQLAS
                     EALNRSRLITISILLLALLIGILAYLLYNRKQLQNKRKLDAELLVQHELRSLAAIDAE
                     ERERVRIARELHDGLGQQLSAARLTISGLQGIIQPADSKQIELFENATSLLDDAVKEV
                     REVSHSMMANALIKNGLTGAINDFVQKLSSTNTIHADLEIVGMEERLNPTLETVLFRV
                     FQELITNIIRHAKAKQISIQLIRHEQELVLVIEDDGVGFDSSQSKNSEGVGLKNIRSR
                     VEYFKGQVHIDSTPDKGTTITVEIPV"
     gene            complement(13004..15124)
                     /locus_tag="PK281_02630"
     CDS             complement(13004..15124)
                     /locus_tag="PK281_02630"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOY48267.1"
                     /translation="MKLGFSLRMVLLFILLNNTGLIAQIQWAQKLVYDSEVSNKDQFN
                     ARMILGAPDANKSGSLSPRCATLNKQKSSALWLEYLPQDVSQILILENNACGLLVRAE
                     LIDDANSSTVVYEQPANPVDISNRLVVIILPQALHNIKKVKLKFSGSSKDAQIDAVGI
                     SADKTPFSLELLAKEFPSISLPSNQLESTYLINYNPGNTNVLNNKKEFNQLLLGLKPG
                     ESGNLGGKVNSTVDEIAPIISPDERTIYFIRSQHPKNSYYGWNSDEEIWFCTWNDSDS
                     SWNEAQHASLPFNSSEINKVVGIRPDGNTMLIKGAYKNGKYKGSGFSFSYRIPNGWSS
                     PEQIRLRGYENMSRGNYVGSYFANDGKTLLLSFSEKSDSPYNDLYVSFLEKDGDWSRP
                     MNLGDKINTQFGEDTPFLASDGITLYFASDRPGGLGKRDIYLTRRLDDSWNNWSVPTN
                     LGSSINTIADDANYSIAASGNYAYLVSERNSIGGSDVVRIKLKDEIKPNPVVLISGKV
                     KDAKTGKFIPASVSYQLFPDGIEAGIASSDPDSGTYKIVLPYGKNYAFSAAAPGYLPI
                     SDNLDLSSIAKYTEIHRDLFLVPFEKGETIRLNNVFFESAKSELLPNSFPELNKLVDL
                     LQAHPTMKIAINGHTDNVGKPADNLMLSEKRAASVLAFLISRNILPDRISSFGFGESK
                     PLTSNDSEENRAINRRVEFSILAP"
     gene            complement(15190..16209)
                     /locus_tag="PK281_02635"
     CDS             complement(15190..16209)
                     /locus_tag="PK281_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48268.1"
                     /translation="MNHLRICIILSVFILGCKAPKMASYSTKTDLTSKAKFDFNQSFI
                     RENGRGFTVSMNALGQKPKRVALVSFYVDDQGITKVSGTNSTGKTYSTTNSGSDNLRI
                     LGNRFYSAGIPQLVSSFKNYGIEILTPEQFLTDDDKRMAYNDFVVKHTTANKVGEKIG
                     KFFKNMASNGTTIELDEAADGFRLMKINKRDEVDPKKKSIPANNLAGSFDGQMIESIG
                     YDLTRLLEVDAVLIVYNSQLADTKWGKTRFWMSAVNMHMFGPNPLPLKEGKKDNNLYS
                     KGLFYGGVRMAFKKGLVINPKEKDEVKRALLEKQTDQAYANMIAGCVNRLGLYMDKEL
                     KKAKK"
     gene            complement(16327..17040)
                     /locus_tag="PK281_02640"
     CDS             complement(16327..17040)
                     /locus_tag="PK281_02640"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HOY48269.1"
                     /translation="MNRNFSLSIFLLFLSIYPLAAQQSSVRYAGTMRDAMWKGEIFGK
                     INLDTLQHKEHLYGIGPLANLQGEILINAGHAYQSRVSGPGSMQVIESFEGKAPFFVY
                     DRVDHWIQFPLHDSIRTLQQIEQALLEVSKIHPSPFSFLIMAVMDKGSIHVVNLPSGT
                     IVHNPDEAHIGQVDFPIFQTEGQVVGFFSTQHQGIFTHHTSFVHAHFLSNNHQLMGHI
                     DYAEFQPATLKLFLPFSIP"
     gene            complement(17037..17900)
                     /locus_tag="PK281_02645"
     CDS             complement(17037..17900)
                     /locus_tag="PK281_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48270.1"
                     /translation="MKKLALLFQILSISVFSQIQPSELTLNERNNLQLIRELEHFGRH
                     TIKLKLHNHFYKKWQKQEQKNTYLYVSRPDSILLPNGFRPFEFFGTDTAGARLKADSC
                     SKVGYDAMIYHTSGTSAVLLTRYLLNYPPEAVVFVVLHEMAHVYRNSARLKIPYAAEE
                     SFGDFLGNNAGEYFIRKYHPEWLESFLIQKSVHESIYKLLATTEAKVQGIPLEQKNQI
                     YSQTNSDLKEILFRGNQFIIDRFSYPVNHAYLLRNRYYYRWYWQFISEYSHVNSWKEI
                     RKYYQSFPLHE"
     gene            complement(17925..18944)
                     /locus_tag="PK281_02650"
     CDS             complement(17925..18944)
                     /locus_tag="PK281_02650"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HOY48271.1"
                     /translation="MKVLKIIGLSLVGLILLCVIIGFIMPAKSVCERSMEMKASPAVV
                     FDQINTLKNWDKWSPWKEMDPKMVNTYNEIPSGKGAIMSWEGPESGKGKMTITESIVN
                     ALVLTDLEFDGMGVSKCSYKVEPGTAGGSKMTWTMETDNGNNPFMRLLSGLMKGMLES
                     QFDKGLSNISTLTEKMPAMNVSGGGIILKVEEGTQEAFEYLAIHDTASVATIGNKMGA
                     AFGEIEAAIKNQKLEMAGAPFALYYTDSQTNFDFDMGIPVSKSGKAVGRIKPGKMKPG
                     KTVVAIYQGPYENTWKGSEAVMNYLKEHPKLSIAGPMYESYVSDPGMEPDSTKWITNV
                     VFPVN"
     gene            complement(19061..20089)
                     /locus_tag="PK281_02655"
     CDS             complement(19061..20089)
                     /locus_tag="PK281_02655"
                     /inference="COORDINATES: protein motif:HMM:NF013384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low specificity L-threonine aldolase"
                     /protein_id="HOY48272.1"
                     /translation="MNTRFFASDNCSGIHPQIMEAIIQANTGHVKGYGYDPYTREAEA
                     AFRNIFGDDIEVYFVYSGTGANVLALESCIRSYEAVICSDMAHIYTDEAGAPERFLQA
                     KLITLPSTHGKITPEQVLPVLVGRGVEHHVQPKVISLTQSTEYGTLYTSEELRAFGEL
                     AQKEDLYFHVDGARISNAVAALGISAAELIKNAGVDVMSFGGTKNGMMMGEALVFFNK
                     DLAKNFLYRRKQGMQLSSKMRFISAQFSAFFKHDLWLQTAAHANKMAKELEAGLLKTG
                     RVQITQPVEVNGVFAIFPEGVAEVLQQEFPFYVWNEFTREVRLMCSFDTSSEDIDIFL
                     KRLHQLLN"
     gene            20186..21073
                     /locus_tag="PK281_02660"
     CDS             20186..21073
                     /locus_tag="PK281_02660"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HOY48273.1"
                     /translation="MQIKRLIWGIAWLLMCQSCDQSLPETTEKVLTKPTEDSIPKVEK
                     TDTIPIIHYRMLVLNDSVRKQLRTTYPDGKLELICKLNRIDVMRIHRVDSLIIPDTFS
                     VQINDYSPFPDSIPTYSSIRKIIVVSYPLQAFGAYEQGTLVRWGPVCMGKKATPTPIG
                     LFHTNWKSKKQISTENPDWILPWYFNLVNNTGVSFHQFDLPGFPSSHSCIRMGEDDAM
                     WMYSFAEQWILDSSERTIIAYGTPVVIFGTYGFGKERPWYRLAQNAQSTKITADSMIG
                     IIKPFEQTIFERQQFRDSL"
     gene            21110..21478
                     /locus_tag="PK281_02665"
     CDS             21110..21478
                     /locus_tag="PK281_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015363864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HOY48274.1"
                     /translation="MQHLIPYFTIGFVVLIHIYIVWLEMFAWTSRGPKVFKQFPKELF
                     PPTKALAGNQGLYNGFLAAGLIWSLLIENAEWKSNIAYFFLGCVAIAGLYGAATASKK
                     ILMVQFIPALIGIISVWFCW"
BASE COUNT         6619 a         4176 c         4171 g         6601 t
ORIGIN      
        1 cgaacccgcg acctccgccg tgacagggcg gcattctaac cagctgaact accgatccat
       61 tggtattcct tgttcaggga tggcaaaagt atctattttt ttatttgata caacatgtta
      121 gggtaaaaaa ataattgaaa atgtgtttct gcttcattga caggcagatt cattttaata
      181 ctcgttctaa acaattgccg ttaataaatt gaacacattc gttctatcag cgaaatgcca
      241 gcctgaaatg aagcactaac tttgtcaagc ctaaccgaaa taagtatgca acaatatcag
      301 gctttactag cccacattct agaaaacggg acgcaaaaac acgaccgaac aggtactgga
      361 acgattagct gttttggcta tcagatgcgt tttgatttaa gtaaaggctt tccaatcgta
      421 acgactaaga aagtccattt acgctcaatc attcatgaac tattatggtt cttgaaaggt
      481 gataccaata ttgcctacct caaagaaaat ggagttagta tttgggatga atgggctgat
      541 gaaaacggga atctcggacc tgtttatggt caccagtggc gctcttggcc aggtaaaaac
      601 ggtgagacga ttgaccaaat cagcaaactg gttcatatgc tacggaccaa tcctgattca
      661 cgtcgcttaa ttgtgagcgc atggaatgta gctgatgtag atcaaatggc tttacctcct
      721 tgtcattgtt tatttcaatt ttacgtggcg gatggtaaat taagttgtca gttatatcaa
      781 cgtagtgcag acacattttt aggagttcca tttaatattg cttcatacgc acttcttaca
      841 cttatgctag ctcaggtttg tggtttagag ccgggtgaat ttgtccatac atttggagat
      901 gttcacttgt attctaacca ccttgaacaa gctcgtttgc agttaagtag agatccaaag
      961 ccacttccgg ttatgaagat taatccagca gtaaaggagc tttttgattt tacgtttgat
     1021 gatttcacct tggaaggata tgaaccacat cctgccataa aagccgctgt tgctatatga
     1081 tactctccat cgtggttgct accggaaaaa atggggaaat aggaaaagac aatcgattac
     1141 tttggcatct acctgccgac ttaaagtatt tcaagaattt aactactgga catcctatca
     1201 tcatgggacg tcataccttt gagagcattg gaagagcact gcccaatcgg agaaacatca
     1261 tcatcacccg acaactagat tatcaagcgg taggtgttga cattgttcat tcgttggaag
     1321 aagcaattgc tttagtaaaa gatcaacacg aagtgatggt tattggtgga gctgatgtgt
     1381 atcaacaagc attgcccaaa gccaatcgca tttatattac ccgtgtggat attgaaccgg
     1441 aagcggatcg attttttcct gagatagata tgagtgaatg gaatgttcat gtggatgtag
     1501 gatatccggc ggatgaaaaa aatgcttatt ccctgacttt ccagatgtta gaaagaaaaa
     1561 actaacatga acttctctgc tgctgatcgg aaaatactag caaacgttgt atcgacattt
     1621 gttgaaattc ccaatcaagc aacatggatt gattctgttg gcattgcaga aaaaattgca
     1681 gcactgatag ctgaaaaatc gcccgaagaa caagctgatt ttaggcaatt actggattta
     1741 ctggaatcac catttttagg atttacttgg ggcggagcgt ttcgttcttt cgaaaatcta
     1801 agtgctgaag aacgtcaatc actttttcgt tcttggtcag cgtctcgatt tgccttgcta
     1861 cgaaaaggct atcaagcatt aaagaaacta agcactttca tcgcttattc tttgtgtaat
     1921 gaatcaggac agagtttttc tcaaccgata acacagtatc aagcacatca ggaaacatcc
     1981 attaccgaac agcaagctat tccactgatt aaacctggaa taggaatacg ttgtgaagtt
     2041 ttaattgttg gatcgggcgc tggtggaggc gttgcggcag cacgatatgc tcaggctggt
     2101 aaagatgtac ttattgttga aaaaggcccc tggatgcctg aacaagagat gaccaatctg
     2161 gaagcagaaa tggtgagtca attgtacgaa gaacgaggag cattaagcac ggtagatggc
     2221 gccatgacag tgtttgctgg agcttgttta ggtggaggaa caacaatcaa ctggcaggca
     2281 tgtatcaaaa ctcccgattt cgttttagaa gaatgggcag aggaaagtgg aattgcggat
     2341 ttcgtgaaac ctgtttttca atatcaccta gattcggttt ggaaacgcat tggtgctgat
     2401 cgagaagaag taattcataa cactaataat caactgctta tcaagggaag taatttactt
     2461 gggcaggaag ttaacccgat tgcaaaaaat agttcccatt gtaattctga tggtgctcat
     2521 cgctgtggat ggtgtggctt tggctgtaaa caaggcagca aacagagcac actcaaaacc
     2581 tatattcaag acgctattga gcttggcact cgaatattag atcgcaccta tattgaaaga
     2641 ctattattca gtggaaatca agtaattggt gcagaaggat ttcatacaga tcctttgggg
     2701 aataaaacgg aagttcgaat tcattgcgag cgcgtgattg tttcggctgg aagtattcat
     2761 agtccggctt tgttgatgcg cagcgggatt cgtcatccgg aattaggaaa aaacttgtat
     2821 ttacatccgg tagttcctgt tccagcaatc tattctgagg aagtgaaagc ttggagcggc
     2881 gctatgatga caagcactaa tgatgagcac atccgactaa atggtcgcta tggctacaaa
     2941 atagaaactc caccaccaca tcctggatta ttagctttgg gaatgccttg gaaagatgct
     3001 caatcgtttc aggaacgcat ggcccagatt agtcatttag gtgtgtttat cgttttgagt
     3061 cgggatgaat ttagcggagc gatacgatta agtaaagatg gtcgcccgat gattgattat
     3121 tcgctacatg caaacgataa agctcgactg attcatggta tgaaaaacgc tgcacggatt
     3181 catgctgcag ctggggcaaa ggagattgcc ttaccgcata ttcagcccac ttttgttgat
     3241 gcttctgtcg tgaatgacac agttagattt gaaaagctaa ttgatgaaat gcgttgggat
     3301 aaaggtgatt atacgttatt cagtgcacat caaatgggaa cgtgtcgcat gggaacttcc
     3361 tatacacatg atgtagttag tccagaagga aaagtttggg gcgtaaatgg tgtttacgta
     3421 gtggatgcca gtgttttccc aaaaagcagc ggagctaatc caatggtgag tattctagcc
     3481 atcagtgatt atttatgctg tcagcaactt ggataattag aaaatttcga atttatttcc
     3541 ttctactttt agttgctgat aacgcttttc atcaagctga aacaacatag gagctttacc
     3601 actttcttcc catggactta attgttcacc tgtttcaagt attacatcct gacttagaat
     3661 cttgcgacga aagttccgtt tatcgagctc gtgaccgaga atagcttcat acatttcctg
     3721 cacttgcttg agtgtgaatt tctcaggaag tagattaaaa cctgtcagtt gcgtactcat
     3781 tttagctaac aaccgacgat tagcagcttc gataatttct ttgtggtcga aggctaaaac
     3841 aggtaatgat ttcagatcga accattctgc ggcattatgg tcttcgttag gttcaaactg
     3901 ccgacattga tgccaatcaa taaaacagta atatccaata cttacaacac ggccgagtgg
     3961 atggcgagaa gtatcagcaa aggcacggat ttgctttttg taaaatcgct cacatccagt
     4021 aacacgttgc acgatttggt caacatgttc ctcgagagaa tcgttgggat aaacaagctc
     4081 tgcaggtagt gaaaaggtat ttttgaacgg atcgcgtttg cgctttacta acagcacttt
     4141 gattttcttt tcgtgtaatc cgaaaatcac gcaagaaacc gaaaaagcgg ctcgaaaata
     4201 aaagttcaaa tcatccgaag gggcaaaacg caccgatgtc aacttttcca tgaaaaacga
     4261 attttaccta ccagttttgc aaagatataa gatattgaat atcattagtg caaataaata
     4321 gctaacaatg ctatcaaatt aataacgaat agtatcaaat gaatgaacta tccgacaggt
     4381 taatcacctc ggatttgaca aatgaaatct tgaatttgga acaggatttt agaggccgat
     4441 attggctttg aaaatcttga tagaaatggc aagaagaata acaccaaaga atttccggat
     4501 aacaaatagt ccgctcttac ccaatttgcg ctcgaggtag ttaatggaac gaagaaccaa
     4561 gtaaacggcg attaagttga tgaggattcc aatcagaatg ttgtacacac taaaatttgc
     4621 tcgcagcgac atcactgtgg taagcgttcc agatccagca atgatgggaa atgcaattgg
     4681 tatgatgcta cccgatttca catctggatc ttgacgaaat aactcgatac ctagcaccat
     4741 ttcgagtccc atgatgaaga ttacgatgga acctgcaacc gcgaatgaat tgatatcaac
     4801 tcctaaaaca cctaataatc gtgttccaaa aatgaggaac agaatcatga gtaagccgga
     4861 tgcaagcgta gcttttccag attcgatttc ccctagtcga ctcttcatgg aaatgagcac
     4921 aggcacagca cccaccacat caatgatagc gaaaagggtt actgtgatgg tgataatttc
     4981 atccagattg aacagcatgg caatttaggt tgggagctgc aaagatacct aaagcccagc
     5041 tgtttaatga atatcgaggt cgcggaaagt tttttttcct cgaataaaat aatcggctac
     5101 gatagaaccc atataaacac ccgtgctgtt gcgattgtgt tcgacagctt tcagtttacg
     5161 gcgtttttgg cggatagaag gcaacttgcg gtaaaaatgc aaatgcgcac gaataattgc
     5221 gtaaaagtga ctaaatccgt tggtaaacag aaacttaaat gcggcgatac catctaaggt
     5281 catgcgcata aacaagcttc gaaacaagta tttaccttct atgtttttgt agataagctc
     5341 gaggttgttt cggaaattga ggtaggtttt acgccagttc gatttagaaa gtgttcctcc
     5401 accaatgtga aacacgacag atgcaggttc aacgaggatt ttataacccc gatttttcat
     5461 ccgccagcat aaatcaatct cttccatgtg agcaaagaaa agctcgtcaa agccctcaca
     5521 ttcgtgaaac acagaagcac gaacaaacat acaagctcca gaagcccaaa aaacttcttt
     5581 cacatcatcg tattgtccat tatctacctc acattcttca aatatccgtc ctcggcaaaa
     5641 tggaaaacct aaacgatcta aataacctcc agcagcacca gcatattcaa actgattgcg
     5701 ctgtttaaat gctctaattt taggttgaat agcagcaact aaaggatctg actcgaaatg
     5761 tttgataaca ggttcaatcc agttgggtgt aacttctata tcggaattta gcagcacata
     5821 ataatcagca tctacctgtg ctagcgcttc gttatatcca ccggcatagc cattattact
     5881 gggattttcg atgattgtca cactcgggaa agttctccgc atcatcgcca ctgaatcgtc
     5941 tgatgaagca ttatcagcca ccaccacttc ggcattagga gaacaagcaa tgactttagg
     6001 aagaaactgc tccagcagat gctttccatt ccaattcaga atgacgacag cggttttcat
     6061 gcggggcgtt tgtgtttcca acgtttatgt gaccaaagcc agttttgcgg actttcaata
     6121 ataagacttt ccaaggcacg aagatgcatt tcggtgatag caaattcggg ttgagctttc
     6181 ggatcgagac acaactcctg aaattcaagt gtataatgtc cgcgttttac cctgcgcata
     6241 gcgagaaaca caacaacatg accggttgat ttggcgatac gttcggcacc taaaaaaacg
     6301 ggagtatcct gatttaggaa aatagtccag taggcgtttt cgggatgagg agcttgatcg
     6361 gctactaaga gcgagatgac aggttctttg gatgtagcaa tggagcgaaa agtttgttgc
     6421 atcgaaatca actccgagcc atttagctgc ctgatttcct tcatcaaacg atccatcaat
     6481 ttgctggaaa gtggcttgta tgttaccaaa gcacgcatac cagttactga ttttagtcca
     6541 acgcccatcc attcccaatt gcaataatgt cccgatacgg caatgatgct tctcccttgt
     6601 tgcttatagg aatcgagtaa ttcgggattg agaactttta cgcgtgaagg aattaatctc
     6661 ggatttaggc tgagcatttt aaacgattcc accatgatgt cggtgaaatt gaggtagaaa
     6721 cctttggcga tttttagtcg ttcgcgatcg gatttttcag gaaaagaagc ttttaaattt
     6781 gcaagcacca catcgcgtcg atatctcact aaatccgagg caatccaagc taaaaagccc
     6841 gaaaatggat acaacaaaaa gagtggtatc tgagcgatta ggaaacctcc accgcggagt
     6901 aaatagtaaa agaaattcac cggggtgttt gatagttttg ttgaatctga gaaccaaatc
     6961 taccggatgg tgtagtttga tcaatgtcgt tggtttgcgt atcgcgcttg taaatgagta
     7021 attgccactg atcgttcatg atttctccta aagcatcgga gtgaatcaat ttaaaatcat
     7081 tcgttaccaa atcgggattg tagaaaatga atcgacggtt tgattgtcct ttcactttat
     7141 aatactgcca aatctcatac ggatatgcac ttggttcatg cggcatttgc gtgcgattat
     7201 cgggcggacc gtattgcaaa taaacacgtc cgcgatcggt gttatatcct ttacgaatgg
     7261 aagtagaaaa ctccttcacc actttacgta cttcgaaaaa atacttttcc caagcttcct
     7321 gcggatgaag ggcatcgcga ttaatccaaa aggaaagcat gtattgttgc atttgttgca
     7381 aatccttggc tttaatgacg ttagtggcga tgtttttctc taactcatac gaaattggat
     7441 acaaacaacg aatatgttcc gctaaactat ctggattggt ataattgagc acaaaactgt
     7501 taagcaaact agttgatgta taattatcgg gagcaatgat ttcgcctgga tttgaacgaa
     7561 caaacgatac ttttttagta gctaaaacct ggttctctgc atcgcgcact tctaattcca
     7621 aattatactg accagttggc aaattggtga tgtcgaactc tttcatcaac acactaactt
     7681 cggaagctgt atagcgcgta aaacctgaat aagaggtgag ttgctcgttt gtatttgaat
     7741 tgcgcagaaa gaaattgcag aggtattttt ttccttctcc taagaaagta agtgtttgat
     7801 atatttcact gtaaaaaatc agcttcgtct cgtctttcac gaacacatcg ttgatatgag
     7861 gaattaaatc atatccacct ttagataaac tacctggcat ttcggttttg gtgtatgatt
     7921 caactaattg tatgtcggaa aatgataccg cattattagg gaatttaact tccagcggtt
     7981 ctgttgcttc aaccggctcc cctattccat tgacatcgcg aatgcgcaat cctaattcat
     8041 aatttccttc accaagtgga atgcgttgtt gatcgatgaa gttgaaggtg atatgaacag
     8101 aatcgtctgt ttctggactt aagaggttgt atttatcggc ataacggatt tgtccgtctt
     8161 gtgaaacagt aagaatgacc tcgatagtgc cttgatattt tcctgaatcg gttttacgaa
     8221 acaccacgct ggagccacga accatgatgt atgtttcgac gtatggcttg cgtccattca
     8281 tcataaatga ggcatgagaa aaaattgcct gaactgaatt tccgttggaa gcagcaaatg
     8341 ttccgacaga aaagagcaga aatgaaatga ataaaaggaa ttgacgcatg aacgagtatt
     8401 tagacaaaag tacctgattc tatccttgca ttcaacaaca ttttcataag ctcatcggac
     8461 gaggtttgaa gccaattgta gcttgaacgg ctaatttcca accagcatat aattgcgtga
     8521 ttctctgcct ttccgaattg ggtttgaaaa ccttgccttg ttgtgccact tcggaacatg
     8581 cttgagcaga tgtccaaatt ccagcagaaa gtccggctaa caatgcagca ccaagtgctg
     8641 ttgtttcggt gttttgagga cggatgacat ccacttgaag caagtcgctt tgaaattgca
     8701 tcatcagatt attagctgat gcgccaccat cgacaaacag aacagaaatc ttcattccag
     8761 catcttgttg aatggcttca aaaacgtcgg aagtttggta tgccatggct tcgagagcgg
     8821 cacgaatgat atgagatttt tgagttcctc gagttaaacc tacaatcata cctcgagctt
     8881 ccgaatccca atgtggagcg cccaaaccta caaatgccgg cacgaaataa acaccattcg
     8941 tgtcttccac ctgagtggct aattcctcaa tttcggagga atccctgaac aagcctaaac
     9001 catcacgtaa ccattgaatt gccgagccag caacaaagac gctaccttca acggcgtagg
     9061 aaatttgtcc gtttaatccc caagcaatgg tggttaatag tccgcagctc gatttaactg
     9121 gcacattccc cgtattcatc aacataaaac atcccgttcc ataggtattt ttagccatag
     9181 tcggctgaat acaaccttgt ccgaataatg ctgcttgctg gtcgccagct attccatgaa
     9241 tgggaattcc agcatcgaaa taaccatcaa ctgttgtccc aaaatagcct gaactatctt
     9301 tcacttcggg taaaatagat tctggaatat cgaaccattg aaggatttct ttgtcccagc
     9361 aacattcatg aatgttaaat aataaggttc gagaagcatt gctgtagtcg gtagcatgca
     9421 cttttccacc cgttaaattc cataaaagcc aagtatcaac cgttccaaaa gctaattctc
     9481 ctttttcagc acgttgtcgg gcattgggca cctgttctaa tatccaggcg attttactag
     9541 cggagaaata ggcatctacg actaagcctg ttttatcttg aatagcttga ctgattcctc
     9601 gagcgttaat tcgttcacag atttcgagtg attgtcggct ttgccaaacg attgccggat
     9661 agatgggttg accagtcttt ctatcccaaa tgattgtcgt ttcgcgttga ttggttatgc
     9721 caatgcaggt gatttgatca caagaaattc cggcatttag aagcacttgt tgataggaat
     9781 tcttaacgct ttgtaggatt tcgagcggat tatgttccac ccaaccagca tgaggaaaat
     9841 actgcggaaa ctcttcttga gcagttgcaa tgacttgtcc ctgttcgtta aaaattatgc
     9901 aacgagaaga agttgttccc tgatcaatag cgagtatctt tttcaagatt aacggcgatt
     9961 tgagtgaatt gtattgaatg atgggaacga aaatagaaaa taaagcaggt gcgaaatcta
    10021 aatccctttg gctttattga aatcgctcga accaccttta ggaatggaga gtgaaaccca
    10081 agctgaattc tttaacattt tagcaatcca aacaatttgt ccgatatgaa aagtataatg
    10141 tgctacttgg cggtttatgg cttcggtaat ggaatgcggc tcatttcgaa tgaagatgat
    10201 tgtattgaaa ttatcttcat ttacctgatc gagtgcaaaa aataagcaat cccaacccga
    10261 ttcccaatta gctaataaat cggttcgatt agtagttatt ggttcaaatt caagttctcg
    10321 gtttctccat gatttttcgc catcggaatg aagaaaatcg gtccaacgag agcgcatatt
    10381 tccagcgaga tgattgacaa tgatggctat gctattggat tcttcgttgg cacaccaaaa
    10441 aagttcttcg tcagaaagct gaaccattgc cttttcggct gtggttttat attgattaaa
    10501 tatggatatt acggattgga gataatggga agtattcatt ttctgtatgg tgaattagaa
    10561 ataaaattcg aacagaaaat taaaggaaat atttcaattt ttaaataaac acgttgttcg
    10621 gttgaaaata cagtttaata ttttcatttc aatcaatgaa ataaggctgt taatgcaact
    10681 tgtcattaaa tcattcatgt ttgtctacga cgtgttactt tcttgtcttg aaacaagaaa
    10741 gtaaccaaag aagttcaagt ctggacgatg ctgcctaagc acaaggcctt ttttcgacca
    10801 ttctgcttgg acaaacattt ttcttatcaa tatcgaaaca caccctcgcc gcccagacat
    10861 agccatcgca ctgatccgtt tctaaatcta tttttacttt aatataaatt tatttaaagc
    10921 cttctactag aattgaatat tgggtcgttc gataaaacat taatgattta gaatattaaa
    10981 ttttaatcgc ataatgggta ttttatataa atagttaaac aggaatttca acagtaatgg
    11041 tggttccctt atctggagta gaatcgatgt gaacttgacc tttgaaatat tccactcgtg
    11101 aacgaatatt tttcaaccca acaccttctg aatttttcga ttgggatgaa tcaaaaccaa
    11161 ctccatcatc ttcaattact agaaccaatt cttgttcatg gcgaatgagt tgaatgctta
    11221 tttgttttgc cttggcgtga cgaatgatat tggtgattaa ttcctgaaaa acccgaaata
    11281 aaaccgtttc taaagttgga ttaagtcgct cttccattcc aactatttcg agatccgcgt
    11341 ggatggtatt agtagaacta agtttctgaa cgaaatcatt aattgctcca gttaaaccat
    11401 tttttatgag cgcgttagcc atcatggaat gcgatacttc tcgcacttct ttgaccgcat
    11461 catcgagtaa agaagttgca ttttcgaaaa gttctatctg tttcgaatca gcaggttgaa
    11521 ttataccttg aagtccggat atagttaatc gtgcagcaga aagttgttgt cctaaaccat
    11581 cgtgtaattc gcgcgcaatt cgaacacgct ctcgctcttc tgcatcaatt gcagcgagag
    11641 aacgtaactc atgttgaaca agtaattcag catctaattt tcgcttattc tgaagctgtt
    11701 tccgattata taagagataa gccaaaatgc cgattagtaa agctaataac aaaatagaaa
    11761 tagtgattaa tcgacttctg tttaaggctt cagaggcaag ttgattttct ttaagtaaaa
    11821 gagcattttc ctgttctgtt cgctccgttt cgaaacgggt ttccatctca gtgaactttt
    11881 ctgccatact ctcagaaaaa agagagtctt taaccaggga ggatttatcg taatatttca
    11941 atgctagttc tttcctattg gtaagtaggt ataaatcagc gagcgattta aataaagctg
    12001 aacgatttgc actaaccgaa tcacgtagtg ttatttcttg agcttgtagc agatattctt
    12061 ctgctttaga ataggatttt aaatccaaat agacatttcc aatattgcac aaatacagag
    12121 cgatgctgat tgggttttct aatttttgag ctacttctaa agcctttaaa taatacttta
    12181 atccttcttg agttttaccg agtttccgat aataaattcc cacattatta aaagcacctt
    12241 caagattaac cgaatcgcgt tcctcttcac ggatttgaac tgattttaat tcgagttgaa
    12301 gtgctttatt aaaatcccct ttatattctt caaccgtagc taaataatta agtgaatttg
    12361 caatacctaa tttatctcct aagcgtgaaa gtactcgata tgattgattt gcgtatgttt
    12421 caagtttatc gtattgctgc aaattcatgt aaataacaca gagattattt aatgtataag
    12481 cttctttaac ttgatcattt aattcccgaa ataaagcaag tgctttcaat tgtgcatcat
    12541 gagcttcgct taattggttc atttcggtat agcaatgacc aattttgctg taagatgaag
    12601 caattccggg tttatcatta atacgctgac gaatttcgag tgcttttttg ttagttttta
    12661 aagattcacg aaaacgattt ttccgaatta atacaattcc aatatcgttg taggctgagg
    12721 ctatgtagaa atcggaatgg attgattctg ctaatttgag ttccaactga gcatattcat
    12781 ctgatttttc aagcgaaact cctaaatatt cccaagttaa ttcagaatat aaacgcaaac
    12841 gagtagtatc gtcggctgca tttcggactt ggttttcgag atctctaatt actgaatcct
    12901 gcccattaac atgggacaat atcagaaata gaaaaatcga gaagaagaat aatcgcaact
    12961 gtatcataaa aaggtgaaat tacttattcc tgccaagtat taattaaggt gcaagaatac
    13021 tgaattccac ccgtctattt attgctcgat tttcttctga atcgtttgat gttagcggct
    13081 tactttcacc aaatccgaat gaagaaatac gatcaggtag gatatttctc gaaatcaaaa
    13141 aagcaagtac agaagcagcc cttttctccg ataacatcag attatcagct ggtttaccta
    13201 cattatcggt atgcccgtta atggcaattt tcatggttgg atgagcctgc aaaaggtcaa
    13261 ctagcttgtt cagttctgga aatgaattgg gcaaaagttc tgatttagca ctttcgaaaa
    13321 agacattgtt taatcgaatt gtttcgcctt tctcgaatgg aacaagaaat aagtcacgat
    13381 gaatctctgt atactttgca atgcttgaca aatcaaggtt atcgctaatt ggtaaataac
    13441 ccggagcggc tgcagaaaaa gcataatttt taccgtatgg tagaacaatt ttgtatgtgc
    13501 cactatcggg atcagaacta gcaataccag cttcaattcc atcgggaaat agttgataac
    13561 tgaccgatgc agggataaac ttacctgttt ttgcatcttt cactttcccc gaaatgagaa
    13621 caaccggatt gggtttgatt tcatctttca atttaattct aacgacatca cttcctccaa
    13681 tcgagttgcg ttcactcact aaatacgcgt aattcccgga agctgcaata ctataattcg
    13741 cgtcatcagc aatggtatta atgcttgagc ctaaattcgt agggacagac caattattcc
    13801 acgaatcatc taagcgtcga gtaagataaa tatcacgttt acctaatccg ccaggacgat
    13861 cagaagcaaa ataaagtgtt ataccatcgg aagctaggaa tggcgtgtct tccccgaact
    13921 gcgtattgat tttatcacct aaattcatgg gacgagacca atctccatcc ttttctagaa
    13981 agctcacata caaatcgtta tacggagaat ccgatttttc agaaaaggat aaaagcagtg
    14041 ttttaccatc gttggcaaaa taagatccta cataatttcc gcgcgacata ttctcatatc
    14101 cacgtaatcg aatttgctct ggactgctcc atccattggg tatacgatag gagaatgaaa
    14161 aaccagaacc tttatatttt ccatttttgt aggcaccttt aatcaacatg gtatttccat
    14221 cgggacgaat accaactact ttgtttattt ctgaggaatt aaacggcaaa ctagcatgtt
    14281 gtgcttcgtt ccaagaggaa tcactatcat tccatgtaca gaaccagatt tcttcgtctg
    14341 agttccagcc ataataagag tttttagggt gttgtgagcg aataaaatat attgttcgtt
    14401 catcaggaga aataattggt gcaatttcat ccacggttga atttacttta ccacccaaat
    14461 tgcccgattc acctggtttt aaacctaaaa gtaattgatt aaattccttt ttattattta
    14521 aaacattagt attacccgga ttataattaa tcaagtaagt agattctaat tgattacttg
    14581 gtaaggatat gctaggaaac tctttagcta ataattctaa tgaaaaagga gttttatcgg
    14641 cacttattcc aacagcatca atctgtgcat ctttggaaga accactaaat tttagtttga
    14701 cttttttaat gttgtgtaat gcttgaggaa gtataattac taccaatcga ttggatatgt
    14761 caacaggatt agccggttgc tcataaacaa cggttgaaga attcgcatca tctattaatt
    14821 cagcacgaac taataatcca caagcattat tttcaagaat tagaatttga gaaacatctt
    14881 gaggtaaata ttccaaccat aatgcactgg atttttgttt gtttaatgta gcgcatcgtg
    14941 gacttaaact acctgattta ttagcatcag gagcacctaa aatcatgcga gcattaaact
    15001 gatctttatt acttacttct gaatcataca caagcttttg agcccattga atttgagcaa
    15061 ttaaacccgt attattcagt agaataaata aaagtaccat tctcaaagaa aagcccagtt
    15121 tcattttagt atgatttaat cagaacaata aaaaaagtac tccgtttgaa taaacggagt
    15181 acccgaaaat tatttcttag cttttttcaa ttctttatcc atgtataaac caagtcgatt
    15241 tacgcagcca gcaatcatat tcgcgtatgc ctgatcagtt tgcttttcca ataatgcgcg
    15301 ttttacttcg tctttttctt tcggattaat cactaaacct tttttaaatg ccatacgaac
    15361 accaccgtaa aaaagtcctt tactatacaa gttgttatcc ttttttcctt cttttaatgg
    15421 taaaggatta ggaccaaaca tgtgcatatt aacagcactc atccaaaatc gagttttgcc
    15481 ccatttagta tctgctaatt gcgaattata tacaattaaa acagcatcta cttccaacaa
    15541 gcgagttaga tcatagccaa tagattcaat catttgtcca tcaaaagatc cagctaaatt
    15601 attggcagga attgacttct ttttaggatc tacctcgtct cgtttattga ttttcattaa
    15661 acgaaatcca tcagctgctt catccaattc gatggtagtt ccattactag ccatgttttt
    15721 gaagaatttc ccgattttct ctcctacttt atttgcggta gtatgtttta caacaaagtc
    15781 gttatatgcc atacgtttat catcatcagt taggaattgt tctggagtta aaatttcaat
    15841 tccataattc ttaaatgatg aaactaattg cggaattcct gctgaataaa atctatttcc
    15901 cagaatgcgc aaattatcgc ttcctgaatt agttgtactg tatgtttttc ctgtggaatt
    15961 cgtacccgaa accttagtaa ttccttgatc atcgacgtaa aatgaaacca atgctactcg
    16021 cttaggcttt tgtccgagtg cattcatcga cacagtgaat ccacgcccat tttcgcgaat
    16081 aaatgactga ttaaaatcga attttgcctt gctagttaaa tctgttttag ttgaataaga
    16141 cgccatttta ggagctttac aacctaaaat aaaaacagat aaaatgatac atattctcaa
    16201 gtgattcatg atgaacgaag attagcgttt gtaggttgca aatcaccgtc aatcaagcag
    16261 aattggaaat acaccaagag cggtatattc tatccgtact ggtacttaga aaaggctaat
    16321 tttccttcaa ggaatggaaa aaggcagaaa taacttaagt gttgcaggtt gaaattcagc
    16381 ataatctata tgtcccatta attgatgatt attactcaag aaatgagcat ggacaaaact
    16441 agtgtgatga gtaaatatgc cttgatgttg ggttgaaaag aagcctacaa cttgtccttc
    16501 ggtttgaaat atcggaaagt ctacttgtcc tatatgtgct tcatcgggat tatggacaat
    16561 tgttccggat ggtagattaa ccacatgaat agagccttta tccattactg ccattataag
    16621 aaatgaaaat ggacttggat gaattttact tacttcaagc aatgcttgtt ctatttgttg
    16681 aagtgttcta atggaatcat gaagtggaaa ttgtatccaa tgatctaccc gatcgtaaac
    16741 aaaaaacgga gctttcccct caaagctttc gattacttgc atacttccgg gaccggaaac
    16801 ccgtgattga tatgcatgcc cagcattaat cagaatttcc ccttgcaaat tagccaacgg
    16861 acctatgcca tataagtgtt ctttgtgttg aagggtatcg agatttattt ttccaaagat
    16921 ctcccctttc cacatggcat cgcgcatggt tccagcataa cgaacagaag actgttgagc
    16981 agctaaggga taaatggaaa ggaatagcag aaagatagaa agtgagaaat tccggttcat
    17041 tcgtgcaaag gaaatgattg ataatacttc cgaatttctt tccatgaatt tacgtgagaa
    17101 tattccgaaa taaattgcca gtaccagcga taatagtaac gatttcgaag taaatacgca
    17161 tgatttacag gataggagaa tcgatcaata ataaattgat ttccccgaaa tagaatttct
    17221 ttgagatcgg aattcgtttg ggagtagatt tgatttttct gttctagcgg aatgccttgt
    17281 actttggctt ctgtagtagc taataactta taaatcgact cgtgtacact cttttgaata
    17341 agaaatgatt caagccattc aggatggtat tttcgaatga aatattctcc cgcattattc
    17401 cctagaaaat caccaaagct ttcttctgca gcatacggaa tttttaaccg agcagaattt
    17461 cgataaacat gagccatctc atgcaaaacc acaaatacga cggcttcagg aggataattt
    17521 aataaatacc tagtgagtaa aacagcactg gttcctgaag tatgataaat catggcatca
    17581 tacccaactt tggaacaaga atcagctttt aatctagcac ctgcagtatc ggttccgaaa
    17641 aattcaaaag gacgaaaccc attcgggaga agaatgctat cggggcgaga tacatacaag
    17701 taggtatttt tctgctcttg cttttgccac tttttataaa agtgattatg aagtttgagc
    17761 ttgattgtat gtcgtccaaa atgctctaat tcccgaatga gttgaaggtt atttctttcg
    17821 ttgagtgtta attcggaggg ctgaatttga gaaaaaactg atatgctcag tatttgaaac
    17881 aacagagcta attttttcat ccctaagaat tattttctac cgaattaatt aactggaaat
    17941 acaacgttgg ttatccattt ggttgaatcg ggctccattc ctggatctga tacatacgat
    18001 tcgtacatag gtccagcgat agaaagcttc ggatgttctt tcaaataatt catcaccgct
    18061 tcggagcctt tccaggtgtt ttcataagga ccttgataaa ttgctactac cgtttttcct
    18121 ggcttcattt tacccggttt aatacgccca acagctttac cagatttact tactggaatt
    18181 cccatatcaa aatcgaaatt cgtttgagaa tcggtgtagt aaagtgcaaa aggagcacct
    18241 gccatttcga gtttttgatt cttgatagct gcttcaattt ccccaaaagc agctcccatt
    18301 ttgttgccga ttgtagcaac agaagcagta tcatgaatag ctagatactc aaatgcttct
    18361 tgagtgcctt cttcaacttt caaaataatg cctccaccag aaacattcat ggctggcatt
    18421 ttttcggtca aagtagaaat attcgacaat cctttatcaa attgactttc gagcattcct
    18481 ttcatcaaac cacttaacaa acgcataaat ggattatttc cattgtctgt ttccatagtc
    18541 caagtcattt ttgaacctcc tgctgttccc ggttcaactt tgtaggagca ttttgaaact
    18601 cccattccat cgaattccaa atcggttaat actaaggcat tgacaatact ttcggtgata
    18661 gtcattttac cttttcctga ttctggacct tcccaactca tgatagctcc ttttccagaa
    18721 ggaatttcgt tgtaggtatt aaccatttta ggatccattt ctttccaggg cgaccattta
    18781 tcccaatttt taagggtatt tatttggtca aagacaacag ctggtgaagc tttcatttcc
    18841 atggagcgtt cacaaaccga tttagctggc ataataaaac caattattac gcagagaagg
    18901 attagtccaa caagacttaa tccaatgatt ttcaatactt tcatagcaag ggtgggttta
    18961 ggataagcga atctaatgga ttcaaatcag aatgccaaac cgtaagggtt agaaataggt
    19021 aatcagtaga attctaattc attcttaatt taagtttgaa tcagtttagc agttgatgaa
    19081 gtcgtttcaa gaatatgtca atatcttcag aacttgtatc aaatgaacac attaggcgaa
    19141 cttctcgagt aaattcattc catacataaa agggaaattc ttgctgcaaa acctctgcga
    19201 caccttccgg aaatatggca aaaacaccat ttacttcaac cggttgggta atttgaacac
    19261 gaccggtttt caataaacca gcttcgagtt ctttagccat tttatttgca tgagctgcag
    19321 tttgaagcca taagtcatgt ttaaagaatg cactaaactg agcagaaata aagcgcattt
    19381 tagacgataa ctgcatgcct tgttttcttc gatacaggaa attttttgca agatctttat
    19441 taaagaatac caaagcttct cccatcatca taccgttttt agtcccccca aaactcatca
    19501 catctacacc agcattttta attaattcgg cagcagatat tcccaaagca gcaacagcat
    19561 tggatatacg agcaccatcg acatgaaaat acaaatcttc tttttgtgcc aattcaccaa
    19621 aagctcttaa ttcctcagat gtgtaaagcg ttccatattc ggtactttga gttagggaaa
    19681 taactttagg ttgcacgtga tgttcaacac cacggcctac taatactggt aaaacttgtt
    19741 caggtgttat ttttccgtgt gtagatggaa gcgtgattag ttttgcttga agaaaacgtt
    19801 ctggagctcc tgcttcatcg gtataaatat gagccatatc gctgcatatt acggcttcgt
    19861 atgaacgaat acacgattcc aatgctaaca catttgcacc tgttccagaa tacacaaaat
    19921 agacttctat atcgtcgcca aaaatgtttc gaaatgcagc ttctgcttca cgagtatagg
    19981 gatcatagcc atagccttta acatgtccag tattagcttg aataatagcc tccattattt
    20041 gtgggtgaat tccagagcaa ttatcagatg caaaaaaacg tgtgttcata ttttgaaaaa
    20101 tgtccccaaa gatgctcaaa actctctatt aaatacatgc cttttttcct atctgcgtgc
    20161 atcaattaaa cctgtatttt taggaatgca aataaaacgg ttgatatggg gtattgcgtg
    20221 gctattgatg tgtcagtcgt gcgatcaatc tttacccgaa actaccgaaa aggtactaac
    20281 taaaccaact gaagattcta ttcccaaagt tgaaaaaacc gatactattc ctatcattca
    20341 ttatcggatg ttagtattaa acgattcggt tcgaaagcag ttaagaacaa cctatccgga
    20401 tggcaaacta gaacttattt gcaaacttaa tcgcattgat gtaatgcgaa ttcaccgagt
    20461 tgatagttta attatccctg acacattttc tgttcaaatc aacgattatt caccatttcc
    20521 tgattcaatt ccaacctata gttctattcg aaaaataatt gtggtctcct atccgcttca
    20581 agcatttggt gcatatgaac aaggtacttt agttcgctgg ggaccagtat gtatgggtaa
    20641 aaaggctaca cctacaccaa ttggtttatt tcacacgaat tggaaatcga aaaaacaaat
    20701 aagcaccgaa aatccggatt ggattttacc ttggtatttt aatttagtca ataacacggg
    20761 tgtgtcattc catcaattcg atttaccggg atttccatct agtcattctt gtattcgaat
    20821 gggagaagac gatgcgatgt ggatgtattc gttcgccgaa caatggattt tagattcgag
    20881 cgagcgaact attatcgctt atggtacacc tgtagttata tttggaacgt atggttttgg
    20941 gaaagaaaga ccttggtaca gacttgccca gaatgcacaa tcgaccaaaa tcacagccga
    21001 ttctatgatt ggaattatta aaccattcga gcaaactatt tttgaaagac agcaatttcg
    21061 tgattcactc taaaataagg agcaaatttt aactcaataa atataaatta tgcaacatct
    21121 tattccctat ttcaccattg gttttgtagt ccttattcac atctatattg tttggctaga
    21181 aatgtttgct tggactagtc gtggaccgaa ggtttttaaa caattcccaa aagaattatt
    21241 tccaccaact aaagctctgg caggtaatca aggtttatat aatggctttt tagcagctgg
    21301 attaatttgg agtttattaa tcgaaaatgc cgaatggaaa tcgaatattg cttatttctt
    21361 tttgggctgt gtggcaatcg ctggtttata tggagctgcc acagctagca agaaaatatt
    21421 aatggtacag tttattcccg ctttaattgg aattatatct gtttggtttt gttggtaatt
    21481 tttaaattat aattattcaa tttaatgcga attatcacac ccgtccaaga cgtttgtgta
    21541 acgaaaatac aatcctgatt tgatgcg
//
LOCUS       DAOOQW010000022        21139 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_3057_length_21139_cov_6.591444, whole genome shotgun sequence.
ACCESSION   DAOOQW010000022 DAOOQW010000000
VERSION     DAOOQW010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 21139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21139
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3057_length_21139_cov_6.591444"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="PK281_02670"
     CDS             complement(<1..198)
                     /locus_tag="PK281_02670"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HOY48275.1"
                     /translation="MNSFPKEERLSGRTTINEVFSLGKRFTIYPFRIIWRYRTPDKLP
                     IIRFGITVPKKISKKAVVRNRI"
     gene            complement(207..1010)
                     /locus_tag="PK281_02675"
     CDS             complement(207..1010)
                     /locus_tag="PK281_02675"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019386064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HOY48276.1"
                     /translation="MSSTIQATAEEQAENSREQRVKTILVTQPKPETDKSPYFDLAKK
                     CNVKIDFRSFIHVEGISAKEFRKEKINILEHSAIILTSRNAVDNFFRLCTEMRITVPE
                     SMKYFCISESTAFYLQKYVQYRKRKIFYGNQKFVELMDVIKKNKTEKFLLLCSDIHKD
                     EIPEILDEAKINYTKAVIYRTVCSDLSDLSDVLYDMLVFFSPSGIKSLFQNFPDFKQN
                     ATRIAAFGPTTAAAVKEAGLILDINAPMPQAPSMTMAIEQYLKIANKGR"
     gene            complement(1020..1874)
                     /locus_tag="PK281_02680"
     CDS             complement(1020..1874)
                     /locus_tag="PK281_02680"
                     /inference="COORDINATES: protein motif:HMM:NF025459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4271 domain-containing protein"
                     /protein_id="HOY48277.1"
                     /translation="MISPPDSLVHAANSGNLPDSVTVAPAIPVAEIGPPLPVPTFSKP
                     ETEERLPNFFFTHYSKDLQPHPRYSDAEGWIVPTLLGAVFLLGIINTFYFKEVRMILM
                     GIFKRSGISKLLEEENTMIRRSIVMMILLFLVVAPVFAYQISGLLQIQTAYLPYIPAY
                     FQLLLLGAGSLGFKLLTINMIGNLFYAKEESGSYIIGIIVMNCILGVALIPVTLGIKL
                     APAPFNTWALYTGIVLFIALYLYSLIIGWLAGLRNGTLSKFHLFLYFCTLEILPVFLI
                     IKAVRNLI"
     gene            complement(1962..2522)
                     /locus_tag="PK281_02685"
     CDS             complement(1962..2522)
                     /locus_tag="PK281_02685"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY48278.1"
                     /translation="MSPQTDAGNVIGTLAITSTTTTQLKSSGNYVTHKTAGNSGSGSK
                     SWSFHWTAPSAGTGDVPFYASVMAANNNGNENGDATFTDVYTVSEDITASIGSSLESN
                     SEFEINPNPIEGNELHISFSANAGETASIRILSMNGALVKEINHAVSSTGNQSLTVQT
                     DDLAKGVYLIQVQTLAGTKSSRVIRR"
     gene            complement(2574..2807)
                     /locus_tag="PK281_02690"
     CDS             complement(2574..2807)
                     /locus_tag="PK281_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48279.1"
                     /translation="MKKIYYLIGVLGVMTIALMLDSTNTYSEPSGAPERCSGSPADGQ
                     TCANTDCHNGTATERDGMITSDIPSTGYIPGTV"
     gene            3009..3950
                     /locus_tag="PK281_02695"
     CDS             3009..3950
                     /locus_tag="PK281_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/SPASM domain-containing protein"
                     /protein_id="HOY48280.1"
                     /translation="MSFFISRRSGKSIHWGLPMSISIEPTTSCNLRCPECPSGLRAFS
                     RPTGMLQISEFNTFLHQFGKKLWSINFYFQGEPFLNKELLAMVEIAASKGIYTATSTN
                     AHYFNEELAKQTVASGLHRLIISLDGTTQETYSQYRIGGNLEKVIDGTRRVIAAKKAA
                     KSRTPHIIFQFLVVKPNEHQTLDALELAKELGVDEVRFKTAQVYDYENGNPLIPEQEK
                     YSRYRKGSDGKWKLKNKLDNECWRMWQGCVVTWNGSIVPCCFDKDAQHQLGSLKNQSL
                     KQIWNSSDYQNFRLAILKDRSAIDICRNCSEGTKVWA"
     gene            complement(3953..4927)
                     /locus_tag="PK281_02700"
     CDS             complement(3953..4927)
                     /locus_tag="PK281_02700"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOY48281.1"
                     /translation="MTGIEHYQSRISEALERIQFPQEPAELYEPIRYTLALGGKRMRP
                     VLVLMGADLLGGNLDDVINPALGIEVFHNFTLLHDDIMDEAPLRRGKATVHTKWNSNI
                     AILSGDVMFVESCRLVSKAPAKVLSPVLEVFYKAAGDVCEGQQWDMNFEAQENVSLPD
                     YLKMIRLKTAVLLGASLQIGALCANANEKDAALLYEFGCHLGVAFQLQDDVLDVFGDP
                     AVFGKQAGGDILSNKKTYLKLRALEKANPSELAELKNWYSSRSFPAQEKVVAVTRIFE
                     NLQVREDAEKEMNSHLNVALEALKSVNGDPAKIHALEKFAYQLMDRVK"
     gene            complement(4924..5862)
                     /locus_tag="PK281_02705"
     CDS             complement(4924..5862)
                     /locus_tag="PK281_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014166575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOY48282.1"
                     /translation="MTRILITGGAGFIGSCLAEKLASNPENLVVVVDNLRTGELSKVP
                     VSVHNNIRFIKCDANDYRDISAVFFAWRFDYVFHYAALVGVIRTLQNPVAVLDDIAGI
                     ENILKLSKNTGVRRVYFSSSSEVYGEPVEFPQNEHTTPLNSRLPYAIVKNVGEAYLRS
                     YEKEFGLEYTIFRFFNTFGPKQSKDFVISKFIRAALNNDPISIYGDGSQTRTFCYIDD
                     NIEACTNAFYQNMYVNDVVNVGGTIEIPIVEVAKTIIRLTGSSSEIRFVAPLEEGDMT
                     RRKPDTAKMSKLINRPLVGFEEGLLQVVNNTSFILQ"
     gene            complement(5859..6725)
                     /gene="rfbA"
                     /locus_tag="PK281_02710"
     CDS             complement(5859..6725)
                     /gene="rfbA"
                     /locus_tag="PK281_02710"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016991217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HOY48283.1"
                     /translation="MKGIILAGGSGTRLHPLTLAVSKQLMPVYDKPMIYYPLSTLMLA
                     GIREVLIISTPHDLPHFQKLLGDGKSLGMEFSYAEQAIPNGLAQAFVIGEEFIGNDKC
                     ALVLGDNIFYGSGMTSLLQKNNDPEGGVVFAYHVTDPERYGVVEFDDHMNAVSIEEKP
                     QQPKSSYAVPGLYFYDNSVVEIAKNLKPSARGEYEITDVNKKYLEQGKLKVGVLDRGT
                     AWLDTGTFASLMQAGQFVQVIQERQGLNIGCIEEVAWRMKFINDQQLKELAEPLKKSG
                     YGDYLLNLLKGK"
     gene            complement(6725..9076)
                     /locus_tag="PK281_02715"
     CDS             complement(6725..9076)
                     /locus_tag="PK281_02715"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOY48284.1"
                     /translation="MTVFMGWMATKAEMTYALAQILPQDDPEYQAYTSFKERFGEDAN
                     ILVIGIESENLFQKELFNAWKEAGDSIKALPEVRGLLSLSGIYNLQRDDSLQKFQIDP
                     VIKQAPKTQQELDSLKNVILSLPFYQDLLLTKTAEGKYATVMLVTMDQKSIDTKGRKE
                     LIEHLTSYTDKFSQKQNLEVHYSGLPYIRTMNSEKLKKELGMFLWMSLLVTALVLLLF
                     FRSFKAIIFPLLVVAISVVWCVGTMVLLGYKITILTALIPPLIVIIGIPNCIFLTNNF
                     HREYKGHGNKIKAISRVVQRIGNATFLTNFNTALGFITFAFVQSQMLREFGILSSINV
                     MQVFILSVTILPIAYTYIPSPKPKQTKHLDNRWMDGIIEWIIKLVTTRRKLIYLLALA
                     LVAVSAWGMTKIHTTGNFTDDIPRKDKLITDLKFFEKNFHGVMPFEITIDTKKKNGVM
                     KLSVFRKIDQLQKELRTYPEFSKPLSIAEVIKFAKQGFYGGDSSFYSLPTNEEIAFLL
                     PYAQNMDLKKGGVTRSFLDSTRQFTRVSVQMADIGTKEMERIRSEVRPKIDSIFNPEK
                     FDVKLTGTSIVFLKGTDYLVNNLLMSLLAAIVLISGLMYFMFYSFKMLMISAIPNVIP
                     LLFTAGLMGFYGIALKSSTILVFNIAYGITVDSAIHFFSRYGQDLKANNFDIRLAVIA
                     TIRDTSISIIYTSIILLFGFSIFIFSSFGGTIALGFLISVTIFVGLFTNLLLLPTLLL
                     SIDRAFMIKAYKEPLIAVFNEEEDIDLDSLTIRKEPFNHPTED"
     gene            complement(9287..9754)
                     /locus_tag="PK281_02720"
     CDS             complement(9287..9754)
                     /locus_tag="PK281_02720"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HOY48285.1"
                     /translation="MDAAYFSRLFAYNDWANTRFIEVLKNALNSGITIPENALELLSH
                     NLGAQEIWLCRILNIPGGKELFTPKSLPEIEALNQSSTQKWLNIIEKHKNELFDLKVN
                     YKNLEGQTFQNSLAEICTHVSNHGTYHRGQIARALKAAGIQPPSSDFIVFSRI"
     gene            complement(9790..11340)
                     /locus_tag="PK281_02725"
     CDS             complement(9790..11340)
                     /locus_tag="PK281_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013684963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HOY48286.1"
                     /translation="MNKELIINASGNEIVIAQLEDRRLIELNREKSNNSFAVGDIYLG
                     RVKKVMPGLNAAFVDVGYEKDAFLHYLDLGPQVQSLVKYMKQGLANKLPSASLADFKM
                     DSDIRKDGKIADVLQNNSPILVQIAKEPISTKGPRISSELSLAGRYLVLVPFSDKVSV
                     SQKIKESEERNRLKRLIMSIRPKGFGVIIRTVATNKKVAELDQDLNELIERWNLLVQN
                     LPTAQPKTKVLGEIKRTSALLRDILNDSFNSIVVDDQTIFSEVRNYIAHIAPDKEKIV
                     KLHKGKEDVFEAYGVDRQIKAAFGQNVSMKSGAYLIIQHTEAMHVIDVNSGHRSRSDN
                     DQETNAFDCNMEAASEIARQLRLRDMGGIIVIDFIDMHSPNNRKVLFDKLKEDMAQDR
                     AKHTILPPSKFGLIQITRQRVRPELVIETLEKCPTCNGTGEVQPSILITDEIENNLRY
                     IIKEQNEKSVQLWVNPFIAAFLKQGFPSIQMKWWLKYKSWIPVKANASYHFLEYHFLN
                     KQDEEIKL"
     gene            complement(11635..12462)
                     /locus_tag="PK281_02730"
     CDS             complement(11635..12462)
                     /locus_tag="PK281_02730"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOY48287.1"
                     /translation="MTSKQIVVISASLLVGAAVFMLPREGFRKDEEAGTSKMPSADQQ
                     FEEQLAAVKKSSDASALATIEFFEGRLKQADERSKSIWLDSLTAVWDRQMRPGIAAEY
                     VYQKAQLTGSGTDWLSAGKRFLGLSRFFEGEDKAAVSQRSIECLEKAKSLDPENNDVK
                     TQLGVAYVSGSQEPMKGIMLLREAVKADSTNLEAQMNLGFFSMQSGQYDKAVSRFEKV
                     LAIQPELNEVRIYLSDALEAQGDSKKALKVLLELKKTSTDTLLLTEADRRIQRIQTN"
     gene            complement(12472..12759)
                     /locus_tag="PK281_02735"
     CDS             complement(12472..12759)
                     /locus_tag="PK281_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013186302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOY48288.1"
                     /translation="MTKAEIVAEIADKTGIEKVTVQATVEAFMKSVKTNLSSGKNVYL
                     RGFGSFVVKKRATKKGRNISKNTTIVIPEHFIPSFKPAKTFSDKVKKSVKA"
     gene            12812..13960
                     /gene="mutY"
                     /locus_tag="PK281_02740"
     CDS             12812..13960
                     /gene="mutY"
                     /locus_tag="PK281_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014782103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HOY48289.1"
                     /translation="MLRPLKTGGKDTGFLFKRKDFLEMLESLIIAEMERNMDGFSEQL
                     LTWYGASFRPLPWRETKDPYAIWLSEVILQQTRIEQGTPYYYKFLENFPTITDLANVP
                     EDQVLHCWQGLGYYSRARNLHAAAKKVRDEFKGVFPSDYESILSLPGVGPYTAAAIAS
                     IAFNLPYAVVDGNVYRFLSRLFGINTPIDSPAGKKEFQLLAQSLINPKHPGNFNQSMM
                     EFGARVCRPASPDCESCIFRSSCVAYKNASIDSLPVKAAKTKVRNRYFYFLILCDDQE
                     NVLIEKRGANDIWQGLYQFPLIETESSSPAEEILNVLPILKDNSFQIQKVSSELKHIL
                     SHQHLFARFIHIQVANLPHHLFPNAESIPLESLKDRAFPRLITRYLKI"
     gene            14036..14422
                     /gene="ssb"
                     /locus_tag="PK281_02745"
     CDS             14036..14422
                     /gene="ssb"
                     /locus_tag="PK281_02745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOY48290.1"
                     /translation="MAGVNKVILIGNVGKDPEVKVLENGVRVANFPLATTEIYKKDGN
                     RVEQTEWHNIVLWRGLAEVTEKIVRKGATLYIEGKLRSRTWEDKDKIKRYSVEVVADT
                     LTVLSGRREDSSHHEQGEHYSDDQAH"
     gene            14467..15054
                     /gene="gldD"
                     /locus_tag="PK281_02750"
     CDS             14467..15054
                     /gene="gldD"
                     /locus_tag="PK281_02750"
                     /inference="COORDINATES: protein motif:HMM:TIGR03512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldD"
                     /protein_id="HOY48291.1"
                     /translation="MIGRTLISLGFILLLSLGLISCGEEEVTMPRPRGYYRIDLPEKS
                     YQTFESACPYSFEYPKYAVMQLDSNRKGERCWANLVIPKLHATVHISYKDVKNDGGRF
                     IEDSRNLAYKHAAKASQIEEFLIRYPEKKVYGIIYEIGGNAASSIQFHVTDSTSHFLR
                     GSLYFYAAPNADSLAPVLSFVRKDIDHLIETLAWK"
     gene            complement(15029..16117)
                     /locus_tag="PK281_02755"
     CDS             complement(15029..16117)
                     /locus_tag="PK281_02755"
                     /inference="COORDINATES: protein motif:HMM:NF014665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HOY48292.1"
                     /translation="MASPNFLSNSFRPFVKLSLVFTFLVILAGSVVRTTQSGMGCPDW
                     PKCFGYYIPPTDPSQVDFHPQHAYTKGMMVIVNDTLWRATAPFKSGDVFNREQWEKYP
                     KHNYAKFFVRHTWIEYINRLLGAIMGILVLGACITSFFQKTHKRLLVGLGLLLVFFTG
                     FQAWLGALVVASHLAPVKITTHMLAALLLLAIIQWMIELSKPSKSAIESNQADRMTRL
                     LALTAISFTIIQVLLGTQVREAIDIIANELGHEQRELWIERLPSSFLIHRAFSLMVLA
                     VNTFLLLRAMKFGNARQARFAMLSGFITLAEITVGIALAMWDMPEVAQPMHLVLASLL
                     FSAQFAILLASKSAKPSESAITSRPVSQ"
     gene            16230..17036
                     /locus_tag="PK281_02760"
     CDS             16230..17036
                     /locus_tag="PK281_02760"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOY48293.1"
                     /translation="MSNYFHGYTEKEFNRLVEQSSFLGPDIYQNLHIPKSGTLLEIGC
                     GVGAQTRILTRVSPDLKICCLDRNPDQIQLVQNWLNENAQLSEQIQLVCTEFLEWNAE
                     KQFDAAFICWVLEHVPDPASFIKHAAQQLHSGASIHITEVQNSSLYLHPSNPAIENYW
                     KAYCRRQEQLNGDPNVGAKLRNWLFDAGFEHIDIQAHLMLRDRTEPEELRLMMEYWWE
                     LMESVGDSMQDEGLISRSERDAAHEAIQHFHLHPDACFYYTFVQAEARKI"
     gene            17127..18410
                     /locus_tag="PK281_02765"
     CDS             17127..18410
                     /locus_tag="PK281_02765"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015023570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HOY48294.1"
                     /translation="MSQTASITLDGKSFELPVITGAEDEKAIDIQNLRSESGFITLDS
                     GYKNTGATTSEITFLDGEAGILRYRGYPIEQLAERSSFIEVAYLLIHGELPKKDELER
                     FSNAITRHTLIHEDMKRFYEAYPAKAHPMGVVASITCSLSTFYPEPKSGRSAETIELT
                     VIRLLAKFSTIAAWAYKNSVGHPVMYPRNSFNYVENFLHMMFAMPTEDYEPDPVIVNA
                     LDKLLILHADHEQNCSTSTVRMVGSSQANLYASISAGICALWGPLHGGANQAVIEMLE
                     QIRNDGGDVKKFINKAKDKDDPFRLMGFGHRVYKNFDPRASIIKDACDQMLNKLGVSD
                     PMLDIAKQLEEAALNDPYFVERKLYPNVDFYSGIIYRAMGIPTDMFTVMFALGRLPGW
                     IAQWKEMLGNKEPIARPRQIYTGAVKRNYVNIADR"
     gene            18449..19036
                     /locus_tag="PK281_02770"
     CDS             18449..19036
                     /locus_tag="PK281_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48295.1"
                     /translation="MFAQFSQFVKRVFAAPKKEEPVRQEMLVRSYSYKQQYKTWINQG
                     GHEEILKNLYTSYTLNKLQITGDVPMHVFVEGSSRHVVLHYTDSMGKTLFPYLQDFFR
                     DRIMRIGYNLYLSDRQIISRAGHIEQIDRHILRPAVPAFHSGDKLEQLYGHITIKVDY
                     IDDRPLYLQISAEAIQDKAYGKVFPFDELAETIFQ"
     gene            19036..20211
                     /locus_tag="PK281_02775"
     CDS             19036..20211
                     /locus_tag="PK281_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48296.1"
                     /translation="MRKLLLTLVLTFSFACLIAQPLAISTELTENGQVELIQWDGDGF
                     NCVSSEMPMMYTWSIYTNQHRRLFSKWREKRRVKKYNNEEVVYWENGQEPEASKLDGL
                     VFTPARNASWGIFPYWVNLGFSDSDTSIHWQYQEYTTQTFNCRGGAYYNTYFYTSKMG
                     KNSLPDSIFYGSVYRIDTVSKKDTSSCWEYFYNDQEQLTKIVVGDMTQRSYQPAFNRS
                     ILLFEYTAKSQLKRIIGLSDTLNYDSYPLTDSLFGFLKKGFYLSDDEEDDLIDSIQYQ
                     KDGPEISFLLEYEYQDSLLIGSWIWSKDWSNFLHDSIAYNSNGKVISINHLAEVNDIR
                     GTLFNYDSNGRLTSYQEYFTNMEDNELKREFYEQVNLKYIRRKDIPQEEDSSTSSDQ"
     gene            20208..20657
                     /locus_tag="PK281_02780"
     CDS             20208..20657
                     /locus_tag="PK281_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009089875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOY48297.1"
                     /translation="MKRNIGDYVISDESDLIQVEKVHAYLSLESYWAKDIPLEIVKRS
                     IENSLCWGVYFQGEQIGFARMVTDYSTFAYLADVYILEDHRGKGLSKLLMHEIMAHPR
                     LQGLRRIMLATRDAHSLYTQFGFAASENPASLMQIVVPGIYSNTDNQ"
     gene            20707..>21139
                     /locus_tag="PK281_02785"
     CDS             20707..>21139
                     /locus_tag="PK281_02785"
                     /inference="COORDINATES: protein motif:HMM:NF025793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HOY48298.1"
                     /translation="MKKETVPSFNQIYMDLASSLALRSHCVKQKVGAVLTKDTRIISL
                     GYNGPPAGTHNCDEEWPETGCARDRKGSCYLALHAEANAILYAAKNKTDLEGSTLYVT
                     LSPCLSCARIILSVGIKKVIFLNSYAVYKGMDIDEGVEFLRK"
BASE COUNT         6264 a         4202 c         4304 g         6369 t
ORIGIN      
        1 aattcgattt cggacaactg cttttttcga tatttttttc ggaacagtaa tgccaaatcg
       61 aattatgggg agtttatcgg gagttcgata gcgccaaata atccggaatg gataaatggt
      121 aaatcgtttt ccaagactaa agacttcatt gattgtggtt ctacccgaca atcgttcttc
      181 tttgggaaac gagttcatga gctgaactat cggcctttat tcgcaatctt gaggtattgc
      241 tcaatagcca tcgtcataga aggagcttgc ggcataggag cgttgatgtc gagaattaat
      301 cctgcttctt ttacagctgc ggcagtcgta gggccaaatg cggcaatacg tgtagcattt
      361 tgtttgaagt cggggaaatt ctgaaacaaa gatttgatac cagaagggga gaagaacaca
      421 agcatgtcgt acaacacatc cgaaagatcg ctcaagtcgc tacaaacggt gcggtaaata
      481 actgctttgg tgtaattgat ttttgcttca tccaaaatct ccggaatttc atctttgtgg
      541 atatcggagc aaaggagcaa aaacttctca gttttatttt tcttaatcac atccattagt
      601 tcgacgaact tctggttgcc atagaatatt ttacgtttac ggtactgaac gtacttttgc
      661 aagtagaatg ctgttgattc gctgatgcag aaatacttca tcgattcagg aacagtgatt
      721 cgcatttcag tacacaaacg gaaaaagttg tctactgcat tccggctcgt cagaatgata
      781 gcgctatgtt cgaggatgtt aattttttct ttgcggaact ctttagcaga aataccttcg
      841 acgtgaataa acgaacgaaa gtcaattttg acattgcatt tttttgccag gtcgaaatac
      901 ggcgacttgt cggtttctgg ttttggttgg gtaaccaaaa tcgttttcac ccgctgttcg
      961 cgcgaattct ctgcttgttc ttctgctgta gcctggatgg tagagctcat gcgattaact
     1021 taaatcagat tcctgactgc cttgatgatc agaaagacag gtaggatttc aagggtgcaa
     1081 aagtacaaaa acaaatggaa ctttgaaaga gtgccatttc tcaatcctgc aagccatcct
     1141 attatcaaac tatacaggta taatgcaata aacaagacta ttcccgtgta caatgcccaa
     1201 gtattaaatg gagcgggagc tagttttatt cctaaagtaa cagggattaa ggcaactcca
     1261 agtatgcaat tcatcacgat aataccgatg atgtaactac ctgattcttc ttttgcataa
     1321 aagagattgc caatcatatt gattgtaaga agtttaaatc ctagagaacc agcaccaaga
     1381 agtagtaatt gaaagtaggc tggtatatag ggtagatagg ccgtttgaat ttgtaacaaa
     1441 ccagaaattt ggtaggcaaa aacaggggca acaactaaga acagaagaat catcatgaca
     1501 atgcttcttc gaatcatagt attttcttct tcgagtaatt tagatatacc tgagcgcttg
     1561 aagatgccca tcaaaatcat tctaacttct ttaaaataga atgtattgat tatcccaagc
     1621 aagaaaacag ctcccaataa ggtaggaaca atccaacctt cagcatcaga atagcgtgga
     1681 tgaggttgta aatctttgct gtaatgggtg aagaaaaaat taggtagtcg ttcttccgtt
     1741 tcaggttttg aaaaggttgg aacaggcaaa ggcggaccaa tttccgcaac cggaatagca
     1801 ggagctaccg ttaccgaatc aggtaagttt cctgaattgg ccgcatgtac taaagaatcc
     1861 ggtggtgaaa tcatgaaaat taggctgcgc taaagtacga aggccggacg accttgccaa
     1921 ggttggcaga ggaggtccgg ccttaatcgc attttatttt tttagcgacg gataactcgg
     1981 ctagattttg ttccagctaa agtttgaacc tgaataaggt aaacaccttt agctaaatca
     2041 tccgtttgaa ctgttaaact ttgattgcca gtagaactta cagcatgatt gatttctttt
     2101 accaatgcac cattcatact taatatgcga atagaagcgg tttcacctgc atttgcagag
     2161 aacgaaatat gaagttcgtt accttcaatt ggattggggt tgatttcaaa ttcagaatta
     2221 gattcaagag agcttccaat actagcagta atatcttccg aaacagtgta aacgtcagtg
     2281 aatgttgcat ctccgttttc atttccatta ttatttgcag ccattacaga tgcataaaat
     2341 ggtacatctc cagttcctgc agaaggagct gtccaatgga aactccaaga tttacttcca
     2401 gaaccactgt taccagcagt tttatgggtt acgtaattgc cagagctttt taattgagta
     2461 gttgtagtgc tagtaattgc taatgttccg atcacatttc ctgcatccgt ttgaggagaa
     2521 atagcaaatc cccatttagc tactccactt tgagtaatac taactgaaat tgtttaaact
     2581 gttccgggaa tataaccagt acttggaata tctgaagtta tcatcccatc ccgttcagta
     2641 gctgtaccat tatggcagtc agtatttgca caggtttgtc catctgctgg tgaacccgaa
     2701 cagcgttctg gtgctccact tggttcggaa tatgtatttg tactatcgag catcagggca
     2761 attgtcatta cacctaaaac accaatcaag tagtagattt ttttcatgtt tgcttttatg
     2821 ttgattgaag agtgaatagt gcaaatttaa aagaatgttg gtacatagat tcgttaagta
     2881 aggaaatcag ttaaattaac caatgaagta tctttgatgc gatgtttggt cttcccaagt
     2941 accccgattt ctattcttgg attacaactt ttcgtttccc tagattttgg aatttaattc
     3001 tgcttcgctt gtcattcttc atttctagaa gaagtgggaa gtcaattcat tggggattac
     3061 cgatgagtat ttccatcgaa cctactacat cttgtaacct cagatgcccc gaatgtccta
     3121 gtggtttgcg agccttttct cggccaacgg gcatgcttca aatttcagaa ttcaacacat
     3181 tcttacacca atttgggaag aaactctggt cgatcaattt ttattttcaa ggagaacctt
     3241 tcttaaataa agaactattg gcaatggttg aaatagctgc tagtaaaggc atatatacag
     3301 caacttccac caatgcacat tattttaatg aggaacttgc gaaacaaacc gtagcttctg
     3361 gattacaccg attaatcatt tctttagacg gaaccacaca agaaacatac agtcagtacc
     3421 gtattggtgg aaatcttgaa aaagtaattg atggaactcg acgtgtaatt gcggctaaga
     3481 aagctgctaa aagcagaaca ccgcatataa tttttcagtt tctggttgtc aagcctaacg
     3541 agcaccaaac acttgatgcg cttgaattag ctaaggaatt gggtgtggat gaagttcgct
     3601 ttaagactgc tcaagtttac gattacgaga atggaaatcc attaattcca gagcaggaga
     3661 aatattcccg ttatcggaaa ggctcggatg ggaaatggaa attgaagaat aaattggaca
     3721 atgaatgttg gcgcatgtgg caaggatgcg ttgttacttg gaatggctcc attgttcctt
     3781 gttgtttcga taaagatgcg caacaccaat tgggtagttt gaaaaatcaa tcattaaaac
     3841 aaatctggaa tagttcagat taccagaact tccgtttggc aattttgaaa gatcgttcag
     3901 cgattgatat ttgtcgcaat tgtagcgaag gaaccaaagt ttgggcttga gtttacttaa
     3961 ctcgatccat taattgatag gcaaacttct ctaatgcatg aattttagca ggatctccat
     4021 ttactgattt caaagcttcc aaagcaacat tcaaatgaga attcatctct ttttcggcat
     4081 cctcacgaac ttggagattt tcaaaaattc gcgttactgc gactactttt tcttgagctg
     4141 gaaaagaacg tgaactatac cagttcttta attcagctaa ttctgatgga tttgcttttt
     4201 caagcgctcg taatttgagg taggtttttt tgttggataa aatatctcct ccggcttgtt
     4261 tgccaaatac agcaggatca ccaaatacat ctaaaacatc atcctgtaat tgaaaagcaa
     4321 cacccaaatg gcagccaaac tcatataata aagctgcatc tttttcattc gcattggcac
     4381 acaaagcacc aatttgcaag gaagccccta acaatacagc tgttttaaga cgaatcattt
     4441 tgaggtaatc gggaagactt acattttcct gagcttcaaa attcatgtcc cattgttgtc
     4501 cttcacacac atctcccgca gctttataaa agacttctaa tacaggggat aaaactttag
     4561 ctggagcttt agaaactaat ctgcacgact ccacaaacat cacatcaccc gaaagtatgg
     4621 caatattgct attccatttt gtatgaactg tagcctttcc acgacgcaaa ggagcttcgt
     4681 ccatgatatc gtcgtggagc aaagtgaaat tgtgaaacac ttcaatgccc aatgctggat
     4741 tgattacatc atctaaattt cctccaagca aatcggctcc catcaatact aaaacaggac
     4801 gcatacgttt gccacctaac gctaaagtgt aacgaatggg ttcgtacaat tcggctggtt
     4861 cctgaggaaa ctgaattcgc tctaatgctt cagaaatacg ggattggtag tgttcgattc
     4921 ccgtcattga agaataaacg aggtattatt tactacttgt agcaagcctt cttcaaaacc
     4981 aacaagcgga cgatttatca acttgctcat ttttgctgta tcgggtttac gacgagtcat
     5041 atcaccttct tccaaaggag caacaaaacg aatttctgat gaagatccag ttaaacggat
     5101 gatggtttta gctacttcca caattggaat ttcgatagtg ccacccacat tcaccacatc
     5161 attcacatac atattctgat agaaagcgtt ggtgcatgct tcaatattat cgtcgatgta
     5221 gcagaatgtt cgcgtttgcg agccatctcc ataaatagaa ataggatcgt tatttagcgc
     5281 ggcgcggatg aatttagaga tgacgaaatc cttgctttgt ttaggtccaa acgtgttgaa
     5341 gaagcggaaa atggtgtatt ctaagccaaa ttctttttca taagaacgta gataagcttc
     5401 tcccacattt tttacaatcg cataaggaag gcgagagtta agcggagtag tatgctcgtt
     5461 ttgtggaaac tcaactggtt caccatagac ttctgaagac gaagagaagt aaacacgacg
     5521 aacgccagtg tttttcgaca gtttcaaaat attctcaata ccagcaatat catccaaaac
     5581 cgcaactgga ttttgaagag ttcggattac tccaactaaa gcagcgtaat ggaatacgta
     5641 atcgaaacgc caagcaaaga aaaccgcaga aatatcgcga taatcgtttg catcacattt
     5701 aataaaacga atgttgttat gaaccgagac aggaactttt gataattcgc cagtacgtaa
     5761 attatcaact acaaccacga gattttcggg attagaagca agtttttcgg cgaggcagct
     5821 accaataaat ccagcacctc cagtaattag aatgcgtgtc attttccttt gagtagatta
     5881 agtaaataat ctccgtagcc acttttctta agcggttcgg caagttcttt taattgttga
     5941 tcgttgatga atttcattct ccaggcaact tcttcaatgc atccaatatt caacccttgg
     6001 cgttcctgaa tcacttgaac aaattgtccc gcttgcataa gtgaagcaaa cgttccggta
     6061 tccaaccaag ctgttcctct gtcgagcaca cccactttga gtttaccttg ttctaagtat
     6121 tttttattta catcggtaat ttcatattct cctcgagcgc ttggctttag gtttttagca
     6181 atttctacca ccgaattatc atagaaatat agtcctggga cagcgtatga tgatttaggt
     6241 tgttgaggtt tttcctctat cgaaaccgca ttcatgtgat catcaaactc aactacacca
     6301 tagcgttctg gatctgttac atgatacgca aatacaactc caccttctgg atcattgttt
     6361 ttttgcaata agctcgtcat tcctgatccg tagaagatat tatcacccag aactaaagca
     6421 catttatcat taccaatgaa ttcttcacca atcacaaatg cctgagctag tccattcgga
     6481 atcgcttgtt cggcatagct aaactccatc cctaatgatt ttccatcacc caataatttt
     6541 tggaaatgag gtaaatcgtg aggagttgaa atgattaata cttccctaat tccagccaac
     6601 atcaacgttg acaaagggta gtaaatcatg ggtttatcgt aaactggcat tagctgcttg
     6661 ctcacagcta atgtgagtgg atgtaagcgc gttccagatc cgccagcgag tataattcct
     6721 ttcatcagtc ttcggtagga tggttaaaag gttcttttcg gatagtcaag ctatctaaat
     6781 caatgtcttc ttcttcgtta aaaacagcaa tcaatggttc tttataagcc tttatcatga
     6841 atgcccggtc gattgagaga agcaaggtag gtagaaggag taaattggta aataatccta
     6901 caaatatggt tacagagatc aagaatccaa gtgcgatggt gccaccaaac gaggagaaaa
     6961 taaaaatact gaatccaaag agcaagatga tcgaagtata aattatactg atacttgtat
     7021 cacgaatggt agcaatcaca gccaatcgaa tgtcaaaatt gtttgcttta aggtcttgtc
     7081 cgtaacggga gaaaaagtga atagccgaat ctaccgtaat gccgtaggcg atattgaaaa
     7141 ccaaaatggt agaagatttc aaagcgattc cataaaatcc catcaatccg gctgtaaata
     7201 ataatggaat tacgttcgga atagccgaaa tcatcagcat cttgaacgag taaaacatga
     7261 aatacatcaa acccgaaatg agcacaatgg cagcaagcaa actcattagt aggttgttaa
     7321 caaggtagtc ggttcctttg agaaagacga tactagttcc agttagcttc acatcaaact
     7381 tttctggatt gaagatggaa tcaattttcg ggcggacttc acttcggatt cgttccattt
     7441 cctttgttcc gatatcagcc atctgcaccg aaacccgtgt gaattgtcgt gtactatcaa
     7501 ggaacgaacg tgttacacca ccttttttaa gatccatatt ttgagcatat ggaagtaaaa
     7561 aggcaatttc ttcgtttgtt ggtaaactat agaacgaaga atctccaccg taaaaacctt
     7621 gcttagcaaa cttaattact tcagcaatgg agagtggttt agagaattct gggtatgttc
     7681 ttaattcttt ctgaagttga tcgatttttc taaaaaccga aagtttcatc accccattct
     7741 tcttcttggt gtcaatagtg atttcaaacg gcatcacccc gtgaaaattc ttctcaaaaa
     7801 acttcaaatc agttatgagc ttgtcctttc taggaatgtc atccgtgaag tttccggtag
     7861 tatgaatttt agtcataccc caagccgaaa cagctactag cgctaaagcc aaaaggtaaa
     7921 tgagttttct acgtgttgta accagtttaa taatccattc gataatccca tccatccaac
     7981 gattgtcgag gtgtttggtt tgtttaggct taggtgatgg aatgtatgtg taggcaattg
     8041 gcaaaatggt cacactcaga ataaacacct gcattacatt gatggaggag agaattccga
     8101 attcgcgtaa catctggctt tgcacgaatg cgaatgtgat aaagcccagt gcagtgttga
     8161 aattcgttaa gaatgttgca tttccaatac gttgaactac ccgtgaaatg gcttttattt
     8221 tattcccgtg acctttgtat tcacgatgga aattattggt gaggaaaata cagttaggaa
     8281 tcccaatgat gacgattaat ggaggaatca gtgcagttag aatggtgatt ttataaccta
     8341 acaacaccat ggttccaaca caccaaacca cactaatagc aacaactagt aatgggaaaa
     8401 tgatggcttt gaaggaacgg aagaacagca ataacacaag tgctgtcacc aataacgaca
     8461 tccacaagaa catccccaat tctttcttga gcttttccga attcattgtc cggatataag
     8521 gcaaaccgga ataatgaacc tctaaatttt gtttttggga aaacttgtcg gtataggatg
     8581 ttaaatgctc aataagttct ttgcgcccct tggtatcaat gcttttttga tccattgtta
     8641 ccaacatcac cgtagcatat ttcccttcgg cagttttagt taataagaga tcctgataaa
     8701 agggtagtga tagaatgaca ttctttaggc tatctagctc ttgctgtgtt ttaggagctt
     8761 gctttataac cggatcaatc tgaaattttt gaagagaatc gtcgcgctgc agattataaa
     8821 tcccagaaag agataacaag ccacgaactt ctggcaaagc tttaatcgaa tcgccagctt
     8881 ctttccaagc attgaaaagc tctttttgaa aaagattttc tgattcgata cctataacga
     8941 gaatgttggc atcttcacca aaacgttctt taaacgaggt gtaggcttgg tattctggat
     9001 cgtcttgagg gagaatttgg gcgagcgcat aagtcatttc cgcttttgtc gccatccagc
     9061 ccatgaacac agtcattact gtgatcacaa ttaggatcgg taaacgataa cggagaataa
     9121 tgcgtgaaag tgccgcccac atggtgtttc agttcaatct gatgagcgtc tttttggagg
     9181 acagaacatc ctgatatcag gagggcgaag gtagaaaaaa cgtctatacc agcgtgtaaa
     9241 attgacgctc tttgtgggaa tctcgcaaaa aataaaacgc gtttttttag attcgggaga
     9301 aaacgatgaa atcgcttgat ggtggctgaa ttccagctgc ttttaatgca cgagcaattt
     9361 gaccgcgatg ataggttccg tggttactca catgcgtgca gatttcagca agtgaatttt
     9421 gaaaagtttg tccttcaaga tttttgtaat taacctttaa atcgaagagt tcatttttat
     9481 gtttctctat gatattcaac catttttgag tggatgattg gttaagagct tcaatttcgg
     9541 gcagcgattt aggagtaaaa agttcttttc ctcctggaat gttcagaata cggcacaacc
     9601 agatttcctg agcacctaaa ttgtgactta aaagttcaag cgcgttttca ggaatagtaa
     9661 ttccagagtt aagtgcgttt tttaaaactt ctataaaacg ggtgtttgcc caatcgttgt
     9721 aggcaaaaag gcgagaaaaa taagcagcat ccatacggta aaagtaggaa tgctgcttaa
     9781 ttattcgtgt tataatttaa tttcttcgtc ttgtttattc aagaaatgat attcaaggaa
     9841 atgataagaa gcattggctt ttacaggaat ccaagatttg tatttaagcc accatttcat
     9901 ttgaatagaa ggaaatcctt gtttgagaaa ggcagcaata aatggattta cccaaagctg
     9961 aaccgatttc tcattttgtt ctttgatgat gtaacgtaag ttgttttcaa tctcatctgt
    10021 aattaggatt gatggttgaa cttctcccgt tccgttacaa gtagggcatt tttctaatgt
    10081 ttcaattacc agttctggac gaacacgctg acgggtaatt tgaattagcc caaatttact
    10141 tggtggcagg atggtgtgtt tagcccgatc ctgagccatg tcttctttca atttatcgaa
    10201 aagcactttg cgattattag gcgagtgcat atcgataaaa tcgattacga taattccacc
    10261 catatcgcgc agacgaagtt gacgagcaat ttcggaagct gcttccatgt tgcagtcaaa
    10321 agcattggtt tcctgatcgt tgtctgaacg ggaacgatgt ccactgttca catcaatcac
    10381 gtgcatggct tcggtgtgct gaataatcaa gtatgcaccg cttttcatgc taacattttg
    10441 tccaaatgct gcttttatct gacgatcaac accataggct tcgaaaacat cttctttgcc
    10501 tttgtgtagt ttgactattt tctctttatc aggagcgata tgcgcgatat agtttcgcac
    10561 ttcgctgaaa atagtttggt cgtcaacaac gatactattg aaagaatcgt tgagtatgtc
    10621 gcgtaataag gccgacgtac gtttaatttc tccaaggact tttgttttcg gctgagcggt
    10681 tgggaggttc tgcacgagca gattccagcg ttcgatcagt tcgttgaggt cctggtccag
    10741 ttcagctact tttttattgg tagcaacggt acgaatgatg acaccaaacc cttttgggcg
    10801 gatgctcatt atgagccgct taagtcgatt gcgttcttcc gattctttaa ttttctgtga
    10861 tacggatact ttatcactga aaggtacaag aacgagatat cttccagcta atgagagttc
    10921 cgagctaatg cgcgggcctt tggtggagat tggttctttg gcaatttgta ccagaatcgg
    10981 gctgttgttc tgaagcacat cggcaatttt cccatccttt cggatatcgg aatccatttt
    11041 gaaatcggct agcgatgctg atggaagttt atttgccaga ccctgtttca tgtatttaac
    11101 aagggactgc acttgtggtc caaggtcgag gtaatgcaaa aaagcatctt tctcataccc
    11161 tacatcgaca aatgcagcat tgagtccggg cataactttc ttgacgcgac ccaaatagat
    11221 gtcaccaacg gcaaagctgt tattgctttt ttcgcggttt aactctatca gtcggcggtc
    11281 ttccagctgt gcaatgacga tctcattgcc ggaggcattg atgatcagtt ctttgttcac
    11341 gagttttagc ttattccgga ggagtttata caaatgacaa aaaagggcag catgtttaaa
    11401 tggcccttaa gtcattaatt tccggaatga tggaaacgga acagaagctg ttttaccgaa
    11461 tacacgtttc ctgttgatcc tggggaagaa caggaaatga gcattagcgt aacagtctgt
    11521 tacttatttc tttttatgac ggttctttct taaacgcttt ttgcgcttat gagtagccat
    11581 tttatgacgt tttcttttct taccgcttgg cataggaaat tttttaaaat gtgattaatt
    11641 cgtttgaatt cgttgaatac gtctatctgc ttctgtaagc aaaagcgtat ctgtgcttgt
    11701 ttttttcagt tctagaagaa ctttcagtgc ctttttagaa tctccttgag cttcaagtgc
    11761 atcgctcaag taaatccgca cctcattgag ttcgggttga atggcaagta ctttctcgaa
    11821 acgtgaaaca gccttatcgt actgtccact ttgcattgag aaaaatccaa gattcatttg
    11881 tgcttccaaa ttggtagaat cggctttaac cgcttcacgt agtagcataa ttcctttcat
    11941 cggttcctga cttccactca catatgcaac tcccaattgg gttttaacat cattgttttc
    12001 tggatcaagg ctttttgctt tttcgaggca ttcgattgac cgctgagata cagcagcttt
    12061 atcttctccc tcgaaaaagc gagaaagccc aaggaatcgt ttaccagcac ttaaccagtc
    12121 cgttccagaa cccgttaatt gtgctttttg gtacacatat tctgcagcaa ttcctggccg
    12181 catttgacga tcccaaactg cagtaaggga gtcgagccag attgatttgc tacgttcatc
    12241 cgcctgttta aggcgtcctt caaagaactc aatagttgct agtgccgaag catcactgct
    12301 tttttttacg gctgccaact gctcctcaaa ttgttgatcg gctgatggca ttttcgatgt
    12361 gccagcttct tcgtcttttc tgaatccttc gcgaggcaac ataaatactg ctgcaccaac
    12421 tagtaaagaa gccgaaatga ctacgatttg tttgctggtc atcgggtgaa attaggcttt
    12481 cactgatttt ttcactttat cactgaatgt tttagccggc ttgaaagacg ggataaaatg
    12541 ctcagggata acaatcgtag tgttttttga aatgttacgg ccttttttag tagcacgttt
    12601 tttcactaca aagctgccga atccacggag gtaaacattc ttacctgatg aaagatttgt
    12661 ttttacagac ttcataaagg cttctacagt agcctgtacg gtcactttct caattcctgt
    12721 cttatccgca atttctgcaa caatttcagc tttggtcatg gctggttgaa ttttaaagat
    12781 ttatgtttga tttagttttt tgttactgtg gatgttacgt cctttaaaaa cgggtggcaa
    12841 agatactggt tttcttttca aacgaaaaga ttttttagaa atgttggaaa gccttatcat
    12901 tgctgagatg gaaaggaaca tggatggatt ttcagagcaa cttttgacct ggtatggggc
    12961 ttctttcaga cctttaccat ggagagaaac gaaggatccg tatgctattt ggctttcgga
    13021 agtcatcctt caacaaaccc gaatcgagca aggcacacct tattattata agtttctcga
    13081 gaactttcct accataactg atttagcaaa tgtacccgaa gatcaagtgc ttcattgttg
    13141 gcaaggactc ggttattatt cgcgtgctcg aaatttacat gctgcggcaa agaaagttcg
    13201 tgatgagttc aagggagtat ttccttccga ttatgaatcc atcctttcgc ttcccggagt
    13261 tggaccttat actgctgctg ccattgcatc catcgctttt aatttacctt atgctgtggt
    13321 agatggaaat gtataccgat ttctatctcg cttgtttgga atcaatactc ctatcgattc
    13381 tccagctggg aaaaaggaat tccagcttct ggctcaatca ctaatcaacc cgaaacatcc
    13441 cggaaatttt aatcagtcaa tgatggagtt tggtgcacga gtatgtcgtc ccgcttctcc
    13501 tgattgtgaa tcctgtattt ttcggtcaag ttgcgtcgct tataaaaacg catcaatcga
    13561 ttctcttcct gttaaagcag ctaaaacgaa agttcggaat cggtattttt attttctaat
    13621 actatgtgat gatcaagaaa acgtactcat cgaaaaacgt ggggcaaatg atatctggca
    13681 aggattatat caatttccat tgatagaaac agaatcaagt tctccagcag aggagattct
    13741 taatgtgctt cctatcctaa aagacaactc gtttcaaatt caaaaagtct cctccgaact
    13801 caaacacatc ctcagccacc aacatttatt tgctcgattt attcatatac aggttgctaa
    13861 tctaccacac catcttttcc caaatgctga atcaattcct ctggaaagcc tcaaagaccg
    13921 agcatttcct cgacttatca cccgatattt aaaaatctag tctttcaaat ttcatccgtt
    13981 tgagtcaaat tctaaattca ctacatttga aattgatcat ttacggatag atagtatggc
    14041 aggagtaaac aaagtgattc tgattgggaa tgtgggaaaa gatcctgagg tgaaagtcct
    14101 cgaaaatggc gttcgtgtag ccaatttccc cctcgcaact accgagattt ataaaaaaga
    14161 cggtaaccga gttgagcaaa ccgagtggca caacattgtt ctttggcgag gcttagctga
    14221 agtaaccgaa aaaattgtcc gcaaaggagc aaccttatat attgaaggaa aattgcgctc
    14281 ccgaacttgg gaagacaaag ataaaatcaa acgctattcg gttgaagttg tagcagatac
    14341 gcttacagtt ttgtctggcc ggcgagaaga ttcttcgcat cacgaacaag gtgaacacta
    14401 ttccgacgac caagcacatt aatcgttagg ctattctatc ttagccccag atttttgaat
    14461 tgctgtatga tcggaagaac gctgatttct ttaggattta ttttgctgtt atcattaggt
    14521 ttaatctcct gtggtgaaga ggaagttacc atgcctcgac ctcgtggcta ttatcgaata
    14581 gatttacctg agaagtccta tcagaccttt gaaagtgctt gtccgtatag ttttgaatac
    14641 cccaaatatg cagtcatgca gcttgattca aaccgaaaag gggaacgttg ctgggcaaat
    14701 ttggtgattc cgaaattgca tgctactgta catatcagtt ataaagatgt gaagaatgat
    14761 ggcggacgat ttatcgaaga ttccagaaat ctagcttata aacatgctgc aaaggctagt
    14821 caaatagagg aatttctcat ccgttaccca gagaaaaaag tatatggaat tatatacgaa
    14881 ataggaggaa atgcagcctc atctattcaa tttcatgtta ccgatagcac ctcacatttc
    14941 ctacgtggct ctttgtattt ctacgcagca ccaaatgccg attcactcgc accagtcctt
    15001 tcgtttgttc gaaaagatat cgatcacctt attgagacac tggcctggaa gtaatagccg
    15061 actcgcttgg tttggctgat ttcgatgcta acagaatagc aaattgagca gagaacaata
    15121 aactagctaa tacaaggtgc atcggttgag ctacttcggg catatcccac atggcgagtg
    15181 caattccaac cgttatttct gccaaagtga taaatccact tagcatggca aatcttgctt
    15241 gacgcgcatt tccaaacttc atcgcacgta gcaaaaggaa agtatttaca gctaaaacca
    15301 tcagcgaaaa agctcggtga attaggaaac tcgaaggcaa tcgttcaatc cagagttcac
    15361 gttgctcatg ccccagttcg ttagcgatta tatcaatggc ttcacgaact tgtgttccta
    15421 gtagcacctg aataattgta aaactgattg ctgtcaaggc taataagcga gtcattcgat
    15481 cagcttgatt tgattcgata gctgattttg atggcttact taattctatc atccattgaa
    15541 taatggctaa taacaacaaa gctgctaaca tatgtgtggt aatcttaacc ggagctaaat
    15601 gcgaggcaac cactaatgca cctagccatg cctgaaagcc tgtgaagaat acgagtagta
    15661 gtcctaatcc aactagcaaa cgtttatgtg ttttctggaa aaaggaagtg atacaagcac
    15721 ctaaaactaa aatacccata attgctccca agagtcggtt gatgtattca atccaagtat
    15781 gacgcacaaa gaacttcgca tagttgtgtt tgggatattt ctcccattgt tcccgattaa
    15841 aaacatcacc acttttaaaa ggtgcagttg ctcgccataa tgtatcgttc acaatcacca
    15901 tcatcccttt ggtataggca tgttgcggat gaaaatcaac ctgacttgga tctgttggtg
    15961 gaatgtaata gccaaagcac ttaggccaat ccggacaacc cattcccgat tgtgtagttc
    16021 gtacaaccga gccggcaagt atgactaaaa aggtgaaaac taaactgagt ttgacgaaag
    16081 gtcgaaacga attggacagg aaattaggac tagccataag aaagattcgt cgacaaaagt
    16141 acatgagcgt gaatcaggat gttcagtagg tgaataattt atttgagagt agaatgttgc
    16201 attagttatg tttgactaat cattcatgta tgagcaatta ttttcacggg tacaccgaaa
    16261 aagaattcaa tcgattagtg gagcaatctt cctttttagg tccggatatc tatcaaaatc
    16321 ttcatattcc taaaagcgga acattactcg aaattggctg tggtgtagga gcgcaaaccc
    16381 ggatattaac gcgcgtaagt ccagatttga aaatttgttg cctcgatcgt aatccagatc
    16441 aaattcaact cgttcaaaat tggttgaatg agaacgctca actttctgaa caaattcaac
    16501 tggtatgtac tgaatttctc gagtggaatg cagaaaagca atttgatgct gcttttatct
    16561 gttgggtttt agagcatgtg cccgatccag cttcatttat aaaacatgct gctcagcaat
    16621 tacattcagg tgcgagcata catattacag aggttcaaaa tagcagcctc tacctgcatc
    16681 cttccaatcc tgctatcgag aattactgga aagcctattg tcggaggcaa gaacagctaa
    16741 atggagatcc gaatgtggga gccaaacttc gtaattggtt attcgatgca ggatttgagc
    16801 acatagatat tcaagcacat ttgatgttac gtgatcgaac agaacccgaa gaattgcgtt
    16861 taatgatgga atactggtgg gaattgatgg aaagtgttgg tgattctatg caagatgaag
    16921 gtttaatttc tcgatccgaa cgagatgctg ctcatgaagc aattcagcat ttccatttgc
    16981 atccggacgc ttgtttctat tacacatttg tccaggcaga agctcggaaa atttaagccc
    17041 gaattattta cagaattctt catccacatt cattacctaa caagctcttt cataacttgc
    17101 aaacctaatt ttgataactc ttcccaatga gtcagactgc atctattacc ctcgatggaa
    17161 aatcgtttga attacctgtc attacaggag ctgaggacga aaaagccatt gatatacaaa
    17221 accttcgttc cgaatcagga ttcatcacac ttgattctgg ttataaaaat acaggagcaa
    17281 caactagtga gattactttt ttagatggag aagcgggcat tttacgttac cgcggatatc
    17341 ccattgagca attagctgag cgttcttcat ttattgaagt tgcctactta cttattcatg
    17401 gtgaattgcc taagaaagat gagttagagc gtttttccaa tgcgattaca cgccacacgc
    17461 ttattcatga agacatgaaa cgtttttatg aagcatatcc agcaaaggcg catccaatgg
    17521 gagtggtagc ttcaattaca tgctcacttt ctacatttta tccagagcca aaatcaggac
    17581 gctcagccga aacgatcgaa ttaactgtga ttcgtttgct ggcgaaattc agcacaattg
    17641 cagcttgggc gtataaaaac tcggtaggcc atccggtgat gtatccccgt aactcattca
    17701 actatgtgga gaatttcctt cacatgatgt ttgcgatgcc aaccgaagat tacgaacccg
    17761 atccggtgat tgtaaatgct ttagataaat tactcatcct tcacgccgat cacgagcaaa
    17821 actgttctac ttcaaccgtt cgtatggttg gttcttccca agctaattta tacgcctcta
    17881 tttctgcagg aatttgcgca ttgtggggac cgcttcacgg aggtgcaaat caagctgtga
    17941 ttgaaatgtt ggaacaaatt cgcaatgatg gtggagatgt gaagaaattc atcaataaag
    18001 cgaaagacaa agacgatcct ttccgtttga tgggttttgg acaccgagtt tacaagaatt
    18061 ttgatcctcg tgcgagtatc atcaaagatg catgcgatca gatgttgaat aaattgggag
    18121 taagtgaccc gatgcttgat atagctaaac aactcgagga agcagccttg aatgatccat
    18181 atttcgttga acgtaaattg tatccgaacg tcgatttcta ttcaggtatc atctaccgag
    18241 caatgggaat tcctaccgat atgtttaccg taatgtttgc attgggtcgc ttaccgggtt
    18301 ggattgcaca atggaaagag atgttaggta ataaagaacc tatcgctcgc cctcgtcaga
    18361 tatacacagg tgctgtaaaa cgcaactatg tgaatattgc cgatcgttaa caggcacact
    18421 aaagtttccc gacgaacgtt ttctccccat gtttgctcaa ttctcacagt ttgtaaaacg
    18481 tgtatttgct gctcctaaaa aggaagaacc cgttcgtcag gaaatgctgg tacgttcgta
    18541 ttcgtacaaa cagcaataca aaacctggat caatcagggt gggcatgagg agattctaaa
    18601 aaacctctac acatcctata cgctcaacaa actccagatt acgggtgatg tgcccatgca
    18661 tgtttttgta gaaggaagtt ctcggcatgt ggtgcttcat tacaccgatt cgatggggaa
    18721 aacgctattt ccctatttgc aggatttctt tcgcgatcgc atcatgcgca taggttataa
    18781 cctgtatttg agcgaccgac aaattatttc ccgagcagga catatcgaac aaatcgatcg
    18841 gcatatttta cgtccggctg ttccggcttt tcattcgggc gacaaactcg agcaacttta
    18901 tgggcacatt acaattaaag ttgattacat cgatgatcgt cctttgtatc tgcaaataag
    18961 cgcagaagcc attcaggata aggcgtacgg gaaagtgttc ccttttgatg agttggctga
    19021 aacaatcttt cagtgatgcg gaaactctta cttacgctgg ttcttacgtt ttcttttgct
    19081 tgcctcattg cgcagccatt ggctattagt acagaattaa cagagaatgg tcaagtggaa
    19141 ctcattcaat gggatggtga tggatttaat tgcgtttcct ccgaaatgcc catgatgtat
    19201 acttggagta tttataccaa tcaacatcga agattatttt cgaaatggcg tgaaaaaaga
    19261 cgcgtaaaaa agtacaacaa cgaagaagta gtttattggg aaaacgggca agaacctgaa
    19321 gcttcaaagt tggatggtct ggtttttacc ccagcaagaa atgcctcttg gggaatcttt
    19381 ccttattggg taaatcttgg tttttccgac agcgatacta gtattcattg gcaatatcaa
    19441 gaatacacga ctcaaacatt taattgccgc ggaggggctt attacaacac ctatttctac
    19501 acttctaaaa tgggtaagaa ttcacttcct gattctatct tttatggaag tgtatatcga
    19561 atagataccg tttcaaaaaa ggatacttct tcctgttggg aatatttcta caatgaccaa
    19621 gaacaactaa cgaaaattgt tgtgggcgat atgacacaac gttcgtatca gcctgcattt
    19681 aatcgctcca ttcttttatt cgaatacacg gcgaaatccc aactcaagcg catcattggc
    19741 ctgtctgaca cactgaatta cgattcttat cctttaactg attctttatt cggattttta
    19801 aagaagggat tttatttatc tgacgatgag gaggatgatt tgatcgattc tattcaatac
    19861 caaaaagatg gtcctgaaat aagtttcttg ttggaatatg aataccaaga ttctttatta
    19921 atcggttctt ggatatggtc gaaagattgg tcgaatttcc ttcacgattc gattgcgtat
    19981 aactcaaatg ggaaagtgat ttccatcaat catttagctg aagttaacga tatacgtggg
    20041 acgctcttca attacgattc aaatggcaga ttaacttcct atcaagaata tttcaccaac
    20101 atggaagaca atgagttaaa gcgtgaattt tacgagcagg tgaatctgaa atacatccgc
    20161 cgtaaagaca ttccacagga agaagactcg agtacttcta gtgaccaatg aaaagaaata
    20221 tcggcgacta tgtgatttcg gatgaatctg atttgattca ggttgagaaa gtacatgctt
    20281 acctaagtct agaatcctat tgggcgaaag acattccgct cgaaatcgta aaacgaagca
    20341 ttgaaaattc cctttgttgg ggcgtgtatt tccaaggaga gcaaattggt tttgcacgca
    20401 tggtaacaga ctattccact tttgcttact tggcggatgt atacatatta gaagatcacc
    20461 gcggaaaagg attgagcaaa ttactcatgc acgaaatcat ggctcatcct cgattacaag
    20521 gtttgcgtag aattatgctt gcaacccgcg atgcacatag cttatacact caatttggct
    20581 ttgccgcctc cgaaaatcca gcttcgctca tgcagatagt tgttcctgga atttattcga
    20641 ataccgacaa tcagtgattt ctgtcacagg aaggatgtcg tagcaattgt taattttacg
    20701 ctcccaatga aaaaagagac agtaccatct ttcaaccaga tatacatgga tcttgcttcc
    20761 agcttagcgt tgcgctccca ttgcgttaaa caaaaggtag gagcagtcct taccaaagac
    20821 acgcgtatta tttcgctcgg ctacaacgga ccgccagctg gaacgcataa ttgcgatgaa
    20881 gaatggccag aaacaggttg cgctcgtgat cgaaaaggaa gttgttacct tgctttgcat
    20941 gctgaggcaa atgccatcct atatgcggca aaaaataaaa ccgatttgga aggcagtacc
    21001 ttatatgtga cactttcacc ttgtctttct tgcgcacgaa tcatcctttc tgttggaatt
    21061 aaaaaggtga ttttcttaaa ttcctatgct gtttataaag gcatggacat cgatgaaggc
    21121 gttgagtttc ttcggaaat
//
LOCUS       DAOOQW010000023        20662 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_3144_length_20662_cov_5.511525, whole genome shotgun sequence.
ACCESSION   DAOOQW010000023 DAOOQW010000000
VERSION     DAOOQW010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 20662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20662
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3144_length_20662_cov_5.511525"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1422)
                     /locus_tag="PK281_02790"
     CDS             complement(<1..1422)
                     /locus_tag="PK281_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48299.1"
                     /translation="MSNANFTVSPATPILTYPNGGETFYSGMDYQITWNQNTLYTYAR
                     LDYSLDNGATWVNIVNGASNYGYYYWNCPNVSSTTALVRISNYQDLSVVDVSDAVFTI
                     KPAVTILTPNGDDGTTLWGGCTITSITFERSPAWSNYRIEYSTNGGSTWNLVVASWST
                     TANPATYNWTMPNLNNVPTLVRVYPTSYSIYADQSDNTFTIRKPVTIIQPNFGGIMQV
                     GTNYNIQWTSDGISNLYDIFYSTNGGTSFTNIVMGYNTSNNTYTWNVPAIPSNNCRIW
                     VRDNVNNCKSDTSDIAFIISSSAPPITLQTPNGISDTLHGCTNKLVTWSETSTFGTYD
                     LHYSTNGGSTWTAIVTNYATTSHQYSWVVPNINSSTVLLRVRQAGSTTNFDLSDAYFT
                     IEPGALVAAPALTTLCPGQNVQLVATGGSNYSWSPATGLSSTTIFNPVASPTVSTQYI
                     VQSNNAGCILADTVQVNVTPASTN"
     assembly_gap    1440..1539
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1540..3124)
                     /locus_tag="PK281_02795"
     CDS             complement(<1540..3124)
                     /locus_tag="PK281_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48300.1"
                     /translation="MKLIKQFLTICFLLFASLVAHTQSVTVVAPNGGEILYSCQQYPV
                     TWTQTGSPSNYWNIDYTLDGGTIWASVTSNYLSTNGTFLWTVPNVQSSTVKLRIYDAQ
                     HPTTVDQSNANFTINIPVILTSPNGSETWQGNTVHNITWNAQGTSNTYNIAYSTNGGS
                     TWTTIVSSYSTASATYAWTVPTIALSTNCLVRVMDAVTNCMMDVSNLPFTITPSTPII
                     LTPNGGETINIGCDYQINWNQNTLYTYARLDYSLDNGATWVNIVNGASNYGYYYWNVP
                     TSITPSTTCLIRLSNYQDLSVWDVSNATFTIPAPVTVTSPNGGETLVGCATASIAWTK
                     TTCLGTWNLAYSTNNGSTWTDIANQSDNGNANQTYNWTVPNGITSSQVLVRVRSYNYT
                     SISDQSNAVFSIIPSNDITVTSPNGGENWIGLSSHTITWTNLPSASGFYTVQYSSNAG
                     SSWNTLATNITGNSYTWAVPNSPSTQYLVKVIDYQNQCKFDVSNANFTVSPATPILTT
                     PNGGETINIGCDYQINWNQN"
     gene            complement(3378..3920)
                     /locus_tag="PK281_02800"
     CDS             complement(3378..3920)
                     /locus_tag="PK281_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015805936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HOY48301.1"
                     /translation="MHSTPTRKLKLYIAMSLDGYIAGPNDELDFLSQVEMEGEDYGYS
                     KFCQQIDTVIIGKRSFDKVVDMGFEYPHLDKPVYIFTRQNLPAKGNCIYYSGDLVQLL
                     KQIRTQQGKHIYCDGGAELANQLLELQLIDEIILSIIPVLLGKGIRLFRETLSSSRLE
                     LKESKTYSSGLVQLHYARKV"
     gene            complement(4126..>5223)
                     /locus_tag="PK281_02805"
     CDS             complement(4126..>5223)
                     /locus_tag="PK281_02805"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HOY48302.1"
                     /translation="LNEKIQKYQGQIDEMGEKPINPVKEIVLTFSSETGGSLTIRPTY
                     LVNNASWSPMYDIKVINTAQPVNILYKAKIVQNTGFEWKDCKVTISTANPSVNNDRPI
                     MNPRYIDYYVYRVQSTVPLSAGISMNMMQINSPVRKDAQDKEQEDSFVDAFDYNAEVS
                     ESEINVEFAIDARQTIKSDGKEHICAIQSYTVPATYKYHTVPKLEQAAFLLAKITDYG
                     KYNMLSGPANIFFDDMYIGQTHLNPKVSSDTLLVSLGRDERIVVKRIRVLDKNSKRFI
                     ADSQKDVYTWETIIRNNKGTPIDIEVLDQVPLSRRDEIKVELNDKQGAEYTEEFGKLF
                     WNYTVKPNDSKKIRFSYTVEYPKGKTVQEQP"
     assembly_gap    5224..5323
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5324..5909)
                     /locus_tag="PK281_02810"
     CDS             complement(<5324..5909)
                     /locus_tag="PK281_02810"
                     /inference="COORDINATES: protein motif:HMM:NF024987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4140 domain-containing protein"
                     /protein_id="HOY48303.1"
                     /translation="MKSHFALVLLLLAGLYSKADEIPTTSKITDVTVFLRQAKETRSA
                     TVTIPKGNSDVVLSGISLEMIDQSLQVSVKGPGTLITATSRINYFETENSIPKDPKLT
                     RLNDSILVFSKDVAWINEQKAVLNGEMSIFAANTKLGSTQEGLKPAELITLLDFYRTR
                     QTDIRKKLFDLTNKEEKLNEKIQKYQGQIDEMGEK"
     gene            complement(6030..6710)
                     /locus_tag="PK281_02815"
     CDS             complement(6030..6710)
                     /locus_tag="PK281_02815"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOY48304.1"
                     /translation="MEHELKIKMNEKLFLRNPEETVLGRKIVHHGLLLINKLGFENFT
                     FKKLATEIETTEAGIYRYFENKHRLLVYLITWYWSYLEYKVSYTINNIQDPAIKLKNI
                     IRVLVEEPRNAGFTSDFISEQDVYQLAIWEGSKSYLTRHVIQDNQDRFFKPYKDLCEH
                     IAQILLEFNPNYPFAHSLASTVLEMSHSQKFFLENLPALTDFTIKDKDNKIVEFLESI
                     VFNSIQVK"
     assembly_gap    6823..6922
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6923..8839
                     /locus_tag="PK281_02820"
     CDS             <6923..8839
                     /locus_tag="PK281_02820"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HOY48305.1"
                     /translation="LNHFFEVVTLQKGISKIVLDFSFSIISIVFGLIILPLYNTWFFV
                     LTLLLGAFFYFIIRYYGKAGIETNIQTSNQKYRLMDLFQQDKPLESSDQSEFLSCIDE
                     TLGAYFKERKGHYGVLEKQYKGIVFFKVLFIGILLFLGALLVQQGKLNIGQFVATEII
                     IFLVINSIEKLIVSLNSFYDIITALYKLEKIVADSPEYSLFSKKKFHLPSFESIYIRP
                     YSRAIKYFIYAICLSGLILLCMPWTQNIESTGTVSTLNPASRPQEVTSRISGRVEKWF
                     IRDGDRVKKNDTIAYISEIKEEYIDPELVSRSESQIRSKESAMESYEQKINAVNAQID
                     ALNRSLRMKLEQARNKIVQGKIKVVSDSIEAHTAQANFKVAETQLKRYEELLSKGVIS
                     RTDMENRRIKLQEALAKKTSAENKFFGTKNELLNTEIEFNSIQQEYAEKLMKADSEKY
                     SVMSALFEAEGSLTKLQNQLVNYSMRQGFYYVLAPQDGYIQSTAIKGIGEIVKEGASL
                     CSIIPDQSEQVVELYVEPMNLPLIRKGQHVQLTFDGWPAFVFSGWPGMSYGTYTAEIV
                     AFDRSISANGKFRVLAKSIGEPWPDAVRIGSGVQGFALLGNVPLFYEMWRQINGFPPE
                     FYTQSTKKDQDVKK"
     gene            8868..10232
                     /locus_tag="PK281_02825"
     CDS             8868..10232
                     /locus_tag="PK281_02825"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOY48306.1"
                     /translation="MLFPSSEVVSQVLISYEKFMDDVMKFHPLSDRAENFKKLGMYQL
                     QAARGSYDPFLEGNHENKFYNGTNYYSVSSAQLKQALFTSQYLKAGYDYGYGNYINPE
                     LKTSTAGIPYLGMEVSLLQGLVIDKRRAEVLKSRAYLNYYTAEQKNQLNQLFFESSQQ
                     YFDWLFSLKQISLNAYFLQLAQQRYTGIESLAEVGERPAVDTVEASLFIQSRLLDLQN
                     AELDKIKSQNQLASFNWNENGNASLQYSFIPSDSLDAYFEKAKQVIGKLMFQDSLSNP
                     IISKYTAFQEVLNVDKRLKREFIKPKLNVNYNLLAGNTNASSPIFTTNSYKWGLSFSM
                     PLLLRTPVNEYRMADISSKNNSLELSSKNNELMYKLNQASQSISVLSQQLLNAQKSVD
                     YSRKLVDAEKQKFEAGESSLFLINTRESKWLESELKLAEYKLKFIKSTLQIIYLKGNL
                     NYVF"
     gene            complement(10239..11162)
                     /locus_tag="PK281_02830"
     CDS             complement(10239..11162)
                     /locus_tag="PK281_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HOY48307.1"
                     /translation="MQYTLHQLQVFLKVTQTLSITKAAEELHLTQPAVSIQLKNFQDQ
                     FEIPLTEIIGRKLYVTEFGNEIAQAAEKILQEVYAINYKTLAYKGFLSGKLTISIVST
                     GKYIMPYILSDFFKKHPGIELNLDVSNKVKVLEKLERNETDLALVSILPESIQTEKLE
                     LMENKLFLVGNKDYSPSSKPKSPNYLEELALIYRELGSGTRQSMEQFFQKNQLYVRKK
                     LELTSNEAVKQAILAGLGCSIMPLIGIKNELMNGDLSILPVKGLPIKTSWNLIWLKGK
                     KHSYVAQAFLNHLKENKTQIVNEHFHWTKAY"
     gene            complement(11223..12053)
                     /locus_tag="PK281_02835"
     CDS             complement(11223..12053)
                     /locus_tag="PK281_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003444134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48308.1"
                     /translation="MSAFKNRPLVIATMHHKEKVIAPILEQHLQVQCFLPDGFNSDVY
                     GTFSGEIERNNSPLNTAREKCLAAMKLTNCDLGIASEGSFGPHPSYLFIPANEEIIVL
                     IDDKNKWEIYARIIHTKTNFASEEIKSESELSSFLNQVHFPSHGIILKSESEIYKGLH
                     DEMKVRKLFRQMNIRTGKVRIETDMRAMHNPTRMESIQLACEELLKKINSLCPSCKTP
                     GFVITRVETGLACSHCGMPSNQIKSKIYACLTCGFNQEVDLVTSNEKADPMFCNYCNP
                     "
     gene            complement(12053..12346)
                     /locus_tag="PK281_02840"
     CDS             complement(12053..12346)
                     /locus_tag="PK281_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48309.1"
                     /translation="MEKQPILQDKDNKKAMKKQLISGHFSSQDALQLITEMIHVKIRF
                     HENKINHLLNEEDIKMREKRIQALQKDLYDIRKEIEKSNGKVELQSELNIVRK"
     gene            complement(12309..12614)
                     /locus_tag="PK281_02845"
     CDS             complement(12309..12614)
                     /locus_tag="PK281_02845"
                     /inference="COORDINATES: protein motif:HMM:NF037786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48310.1"
                     /translation="MHWGGSNPDELAGKISELNNQELHLSMITNRGIKVWPEGFKETF
                     CTDHWRCRFVSNESASISKESIIDILQHAFKKNIDTIKTENLYEFDGKTAYSAGQGQ"
     gene            complement(12765..13745)
                     /locus_tag="PK281_02850"
                     /pseudo
     CDS             complement(12765..13745)
                     /locus_tag="PK281_02850"
                     /inference="COORDINATES: protein motif:HMM:NF012407.2"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
     gene            complement(13867..15579)
                     /locus_tag="PK281_02855"
     CDS             complement(13867..15579)
                     /locus_tag="PK281_02855"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY48311.1"
                     /translation="MKHILCFLLLQQAYLLNAQVTAPCGNDYGSDVNRANIQQQMFTG
                     TVSDVATAIQQAKETRGISLGCPQVEYGPFNAPNITEPTLSSIANVWNSGYRPTLEAY
                     VTACPEPARGMPRYGFAALYARMAGYYSNLTSLEQMGNMLEAQQYSTQHAPEPLATYA
                     GMFGYYHGLTGDPCQLSGVVGTSVDQFCNAVPSYCIPYNAGLYSGETFVVNDHIIQDG
                     NILGDIGGAGYDQGWAVAFLVENAIQQTDPIVKQRYRDAAVLGAEWCIQHPLVTNHNY
                     TAKLVWALAQLYAWTGRSDFKAALIDRLDRNLCPGILTDWDQNGMVDNMNTTIAFSSL
                     YASAQTPGRNWDAHNAQPWYQSMIAWALTEAYVAFRDRGDIALAAQYKPYALLVTDNL
                     ATEILQRGVMETNQTGWVDTPHALLLACWKIARYENENHSNWFDAIWAIWNSGVFNTL
                     GDRGFNIPLYLLLKENIAYVPLAVREEFPLVFNTSTSSYDPIKLYPQPAQNQLKIELD
                     QSIKIQKWMIYSIDGKLISSKILKGESSFSIELPNINNGMYFIELYSQNQGILSKRFQ
                     VFSK"
     gene            complement(15626..16333)
                     /locus_tag="PK281_02860"
     CDS             complement(15626..16333)
                     /locus_tag="PK281_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48312.1"
                     /translation="MQSKNTIMQNRIILLFLLTALTLAFPCFSQISKSRNINLSIATI
                     FNHPYISNQNSSDRFTSNKTTPGYSIELGIDSAILDWLRLDFAINFNQYNTNFSAIHS
                     GLAGGTSTQVDITKSTLGIVLYPFRIIRKNVRFQVGFEYSRLINEQFSGYVHNSFIFK
                     PPTNYDLNKIYSRYSYFANFGIRLKAGYTFRLNNHIAICPYYSLFWTGWMEYEKAPFF
                     IKSIRNSFGLTCLKSFN"
     gene            complement(16343..17212)
                     /locus_tag="PK281_02865"
     CDS             complement(16343..17212)
                     /locus_tag="PK281_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOY48313.1"
                     /translation="MSHQETAVRTTWFSIAGNTAMALIKGAGGYFGNSYALVADAIES
                     TTDIFASILVLLGLKYAARPADENHPYGHGRIEPLITFLVVGFLISSAFYIAHESVAN
                     IGTPHELPKPYTLWILGGIILWKEISYQLVIRKSRETNSSSLKADAWHHRSDAITSIA
                     AFIGISIALIMGKGYESADDWAALFASGFILWNSYKIFRPALGEIMDEHLNDDLIKEI
                     RIHAIEVNGILGTEKCFIRKAGMQYHVELHAMVNGKISVNEGHTLAHQLKDHLRNTIP
                     QLGHVLIHIEPSH"
     gene            complement(17248..17643)
                     /locus_tag="PK281_02870"
     CDS             complement(17248..17643)
                     /locus_tag="PK281_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48314.1"
                     /translation="MYLTRIASGILIFLFSASLLFHFLVLGNVIPYEMVWGGRIENRE
                     QLIQFETFSIISNIIFLVIILKKAQILAWNISPKAVRIGLWVIMIMFFLNTLGNLLSL
                     NSTEKWIMTPLTAITALLTAILIFTKKQD"
     gene            complement(17657..18289)
                     /locus_tag="PK281_02875"
     CDS             complement(17657..18289)
                     /locus_tag="PK281_02875"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOY48315.1"
                     /translation="MFPFTSNILLGNSLLEKFPSGILAPQSSEIQYTDSCQGVMIQFS
                     LTNPVSGQYVDWNFDDPSSGTNNYGVGNTAPHTFFNKGYYHITVYISTSSGIDTLIRT
                     IHIDSCQSKQNPIDPEKCSFEISNVITPNNDGKNEFLEIKSLCQFELFEISIYNRWGV
                     VVYKSTDSNEYWNGKNQQEDCPDGNYIYLLKYKFSNTDETTKQGNILLLR"
     gene            complement(18481..19053)
                     /locus_tag="PK281_02880"
     CDS             complement(18481..19053)
                     /locus_tag="PK281_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48316.1"
                     /translation="MKHTILLLLIICQLFVSAQNDETKLQQRRFFFGVSTGISSIQLS
                     SKAIGNEQQIGLSFPNIKIGKMLSDHSAILLCLPGSIYQEKHMGRTRDRGFEGIIPSY
                     QYWFSNRYWVLGGAGLTFDAPAFYDIKDETERKFYFGPSVLVGTGYEFYKKGKMSVDI
                     QGRMQYGFSNYPTGNRTGLAFNLLIGVNWH"
     gene            complement(19122..19694)
                     /locus_tag="PK281_02885"
     CDS             complement(19122..19694)
                     /locus_tag="PK281_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOY48317.1"
                     /translation="MLAARLKSSFDPYFKAPIQAWEQFLELCEPVAFKKNEIIKQAGI
                     AEKAGYFILSGSGGVFVWKENNYVCLDIMYEDQFFADYMSLITHQASPLETISLEDSE
                     MLKIKSENIEKLKQTPMGQLLFLISAESTFVDKQQQQIDLLLKTAEQRYREMLSKHPD
                     IILRTSQKHIASYLGITTQSLSRIRKKITQ"
     gene            complement(19771..20532)
                     /locus_tag="PK281_02890"
     CDS             complement(19771..20532)
                     /locus_tag="PK281_02890"
                     /inference="COORDINATES: protein motif:HMM:NF025464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparin lyase I family protein"
                     /protein_id="HOY48318.1"
                     /translation="MKKLLGILLILLGSSCNKEKLFDGPDSFNDDFESYQTLDEMLFD
                     NDVKWSFTQNTYSGNLIEVDSSFAHTGSKSMHFKASKSTNEHGASKASIAKQNMAFWE
                     NEILVVEGWYYLAGNDSANWLFLLDLEEQTAIGAGPGMRLELVNGAIRVEHKYNNPDI
                     LQLSSTPVKMPRNQWVHIKFETKLSQKKKGYVKVWQDDVLIIDQSNWKTLPKDILYFQ
                     QGTKGMYSSIEFGITANTHDSDMEIWVDDIQIWKK"
BASE COUNT         6107 a         3709 c         3802 g         6744 t
ORIGIN      
        1 attcgtagat gcaggtgtaa cattcacttg aactgtatca gccaaaatac aaccagcatt
       61 attcgattgt acaatgtatt gggttgaaac tgttggacta gcaactggat tgaaaatagt
      121 tgttgaactc aatcctgtag caggtgacca cgagtaatta cttcctccag tggcaactaa
      181 ttgaacgttt tgtcccggac ataaagtcgt taaagccggt gcagctacta aagctccagg
      241 ttcaatcgta aaatatgcat ccgacaagtc gaaattcgtt gtactaccag cttgccgaac
      301 acgtaagagc accgtggatg aattgatatt tggaacaacc caactgtatt gatgacttgt
      361 agtggcatag ttggttacaa tagctgtcca tgttgatcct ccattcgttg aataatgtaa
      421 atcatacgtt ccaaacgtag aagtttcgct ccaagtcacc aatttattcg tacatccgtg
      481 gagcgtatcg ctgattccat ttggtgtttg aagggttatt ggcggagctg atgaactaat
      541 gatgaatgca atgtcagatg tatctgattt acaattgttc acattatcac gcacccaaat
      601 acgacagttg ttcgaaggaa ttgcaggtac attccacgta taagtattgt tggaagtatt
      661 ataacccatc acaatattgg taaagctcgt accaccattg gttgaataga aaatgtcata
      721 caaattcgaa attccatccg aagtccattg gatattataa ttcgtcccca cctgcatgat
      781 tcctccaaaa ttcggttgga ttatcgttac aggtttgcga atagtgaagg tattatccga
      841 ttgatcagca tatatagaat aggaagtagg ataaaccctg acaagtgttg gtacattatt
      901 caaattcggc atagtccaat tatacgttgc cggattagct gtagtactcc aactagctac
      961 aaccaaattc catgtactac ctccatttgt agaatattca attcggtaat tgctccaagc
     1021 tggtgaacgc tcaaaagtga tagaagtaat cgtacatccg ccccaaagag tagttccatc
     1081 gtcaccatta ggtgttagga tagtaactgc tggctttatc gtaaagacgg catccgaaac
     1141 atccacaacc gataaatctt gataattaga aatgcgcacc aacgcagttg tactagagac
     1201 attcgggcag ttccagtagt aatatccata gttagaagct ccgttgacga tgtttaccca
     1261 agtagcacca ttgtcaaggg aataatccaa acgagcgtat gtgtatagcg tgttttggtt
     1321 ccaggtgatt tgataatcca tacccgaata aaacgtctcc cctccatttg gataggtcaa
     1381 aatcggcgta gccggcgaca cggtaaagtt ggcgttagac acatcgaatt tacactggtn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt gttttggttc caattaatct
     1561 ggtaatcaca accgatgttg atggtctcac caccgttagg agtggtaagt ataggcgtag
     1621 ccggcgacac ggtaaagttg gcgttagaca catcgaattt acactggttc tgatagtcga
     1681 taactttcac caaatactga gtagatggtg agttgggaac agcccaggtg tatgagtttc
     1741 cagtaatgtt cgtggcaagt gtgttccaag atgaacctgc gttagaagaa tattgcacgg
     1801 tgtaaaatcc ggaagccgat ggcaggttag tccaggtgat ggtgtgggaa gaaagtccaa
     1861 tccagttctc tccaccattg ggcgaagtaa cagtaatgtc gttggaagga atgatgctga
     1921 acacagcgtt cgattgatca ctaatgctgg tgtagttgta gcttcttacc cgaacaagca
     1981 cttgagaaga agtgataccg ttaggaacag tccagttgta agtctggtta gcattaccgt
     2041 tatcgctttg attagcaata tcggtccaag tagaaccatt gttggtcgag taggcaaggt
     2101 tccaagtacc gagacaggtt gttttagtcc aagcaatgga agccgtagcg catccaacca
     2161 aagtctcacc accattgggt gaagtaacgg taacaggagc aggaatggtg aaggtcgcat
     2221 tggacacatc ccaaacggaa agatcttggt agttcgacaa gcgaatcaaa caagtagtag
     2281 aaggcgtgat ggaagtagga acgttccaat agtaatatcc gtagttggaa gctccattga
     2341 cgatgtttac ccaagtagca ccattgtcga gagaataatc taatcgtgcg tatgtgtaga
     2401 gcgtgttttg gttccaatta atctggtaat cacaaccgat gttgatggtc tcaccaccat
     2461 ttggtgtaag aataatcgga gtggaaggcg ttatcgtaaa cggtaaattc gaaacatcca
     2521 tcatgcagtt agttaccgca tccattaccc gaactaaaca attggtcgaa agtgctattg
     2581 ttggaactgt ccaagcataa gtcgcagaag cggtactgta gctacttaca atggttgtcc
     2641 atgtggaacc accattggta gaataagcaa tgttgtatgt attagaagtt ccttgggcat
     2701 tccaggtgat gttgtgaact gtatttcctt gccaagtttc acttccattg ggtgaagtga
     2761 gtataaccgg aatgttaatc gtgaaatttg cattactctg atctacagtc gttggatgtt
     2821 gtgcatcgta aatccgcagc ttaactgtcg atgattgcac attaggaaca gtccacaaga
     2881 aagttccatt tgtacttaaa tagttactgg taaccgaagc ccaaattgtt ccaccatcaa
     2941 gtgtataatc gatattccaa tagttcgagg gcgaacctgt ttgagtccaa gtaacaggat
     3001 actgctgaca agaatataag atttcacctc cattgggtgc tactactgta acagattgcg
     3061 tgtgagcgac taaacttgca aaaagtaaga agcaaatggt taagaactgt ttaatcagtt
     3121 tcatattgaa aatagcggtt gtagcgtgtt tgtttttttt gaaaagggac gtaaaattag
     3181 tcattcacac ccattgttgt ttctaaggta tcgatagaaa atatctataa ttggtaaggt
     3241 ttatggcgta atgatttagg aattgtctaa tcaagtttct ctagtttgaa tagataacaa
     3301 atcaaaggaa taatgttcat catcatactc ctttgtcgct atgagttaat gccttaggtg
     3361 tctgataatg tgttaagtta tactttgcga gcataatgta attggaccaa tccactcgag
     3421 taggttttgc tttcttttaa ttccaaacga cttgaagaga gtgtttctct gaatagacga
     3481 ataccttttc ccaataaaac tggaataatg gaaaggatga tttcatcgat tagctgtaat
     3541 tctagtaatt gattcgctaa ttcggcacca ccatcgcagt agatgtgctt tccttgttga
     3601 gtacgaattt gttttaggag ttgaactaaa tcgcctgagt aataaatgca gtttcctttt
     3661 gcaggaaggt tttgtcgggt gaagatgtac acaggtttat cgagatgcgg atattcaaaa
     3721 cccatatcaa caactttatc gaacgagcgt tttccgataa tgacggtatc gatttgttgg
     3781 cagaatttag aataaccata atcttcccct tccatttcga cctgtgatag aaagtcgagt
     3841 tcgtcgtttg gcccagctat atagccatca agactcatag ctatgtatag cttcaatttt
     3901 cgagttgggg tagaatgcat aatctaattt acacgagttt taacgaacat ttgcagctgt
     3961 tgaaaatttt gcgtgagcgg tagaaatgga aagcccgaag tttatgccga agccttggcg
     4021 aaggcataca ggaggacttg caatgtatag cgcgaccgaa gggcacgccc aaataaaaaa
     4081 ggacaagcca ttgctgactt gcccttcaca cacaaaacaa ttcgattatg gctgttcctg
     4141 aacggtttta ccttttggat actcgacggt gtaactgaag cgtatttttt tgctgtcgtt
     4201 cggtttaacc gtgtaattcc agaaaagctt accaaattct tcggtatact cagcgccttg
     4261 cttatcgttg agctcaactt tgatctcgtc gcggcggcta agtggaacct gatcgagcac
     4321 ttcgatgtcg attggtgtgc ctttattatt tcgaatgatc gtttcccagg tgtagacatc
     4381 tttctgagaa tcggcaatga aacgtttgga gtttttatct aacactcgga ttcgttttac
     4441 tacgattcgt tcgtcacgcc cgagtgaaac caataatgta tcgctcgaaa cttttggatt
     4501 taggtgtgtt tgaccaatgt acatatcgtc gaagaaaatg ttagctggtc ctgaaagcat
     4561 gttgtattta ccgtaatcag tgatttttgc taacaagaat gcagcttgtt ctaatttagg
     4621 cacagtgtgg tatttgtaag tcgcaggtac tgtgtatgat tggatagcgc aaatatgttc
     4681 tttcccatcg gacttaatag tttgacgtgc atcaatggca aattctacat tgatttcgct
     4741 ttcactaact tctgcattgt aatcaaatgc atctacgaag ctatcttctt gctctttgtc
     4801 ttgtgcgtct tttctcactg gggaattaat ttgcatcatg ttcatgctaa ttcctgctga
     4861 taatggcact gtagattgaa cacgatacac ataatagtcg atgtaccgcg gattcataat
     4921 aggacgatcg ttattaacac tcggattggc agttgaaata gtgactttac aatctttcca
     4981 ctcgaaacct gtgttttgaa cgattttagc tttgtaaaga atattcaccg gttgagcggt
     5041 attgatgact ttgatatcgt acatcggcga ccagctagca ttatttacta aatacgttgg
     5101 acgaatagtt aaggaaccac ctgtttcgga tgaaaatgta aggacgattt cttttactgg
     5161 gttgataggt ttttctccca tctcatcgat ttgtccttga tatttctgga ttttctcgtt
     5221 tagnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngtttttc tcccatctca
     5341 tcgatttgtc cttgatattt ctggattttc tcgtttagtt tttcctcttt attagtgagg
     5401 tcgaatagct tcttgcgaat atcggtttgt cgtgtacgat aaaaatcaag taaagtgatg
     5461 agttcggcag gttttaaacc ttcttgtgta ctgcctaatt tggtgttagc tgcgaaaatg
     5521 ctcatttcac cgttgagcac agctttttgt tcgttgatcc aagctacgtc tttggagaat
     5581 acaaggatag aatcatttaa gcgagtgagt ttaggatcct tagggattga gttttcggtt
     5641 tcgaaataat taatccgcga agtagcagta ataagtgtac ccggaccttt tactgaaacc
     5701 tgcaaacttt gatcaatcat ttcgagcgag atgcccgata aaacaacatc ggaattacct
     5761 tttggaatcg taacggttgc agagcgggtt tcttttgcct gacggagaaa tacggttacg
     5821 tcggtgattt tggatgtagt cggaatttcg tctgctttac tgtaaagtcc agctaatagc
     5881 agtagcacga gtgcaaaatg tgatttcatt ttctgaagat ttaggtaagg ccgaaattaa
     5941 caggttgtgt gctttttgcg gcgcgcgccc ttgtcaaatt ctaagccaga aagtttatac
     6001 cgaccacttt gccgattaag aaaaaaggtt cacttgacct gaatagaatt gaatacaatc
     6061 gactctaaaa attcaacaat tttattgtct ttgtccttga tggtgaagtc ggtaagtgct
     6121 ggcaaatttt ctagaaaaaa tttttgtgag tgcgacattt ctaagacagt actcgccaag
     6181 gaatgtgcaa aggggtagtt cggattgaat tccaataata tttgagcaat atgctcgcat
     6241 aaatccttgt aaggcttgaa aaaccgatct tgattatctt gaattacatg acgtgtgaga
     6301 taggattttg aaccttccca aatagctaat tgatagacat cctgttcgga gataaaatct
     6361 gaagtaaatc ccgcatttcg aggttcttct actaataccc gaatgatgtt tttaagcttg
     6421 attgctggat cttgaatgtt gttgatggtg taggagactt tgtattccaa atagctccag
     6481 taccaagtaa ttaagtaaac gagaagtcgg tgcttatttt caaagtagcg gtaaatacca
     6541 gcttcggttg tttcgatttc ggttgctaat ttcttaaacg tgaaattttc aaaaccaagt
     6601 ttgttgatga gcagcaaacc atgatgaact atttttctac ccaacacagt ctcttccgga
     6661 tttctcagga atagcttttc attcatttta attttaagtt cgtgctccat gaacaattca
     6721 gaataattat cgtttttatt ttacaaagat agcaatacta tcattaagaa tatagttact
     6781 atctttgcat ttgaatttta atatgaatcc atctcagtta gcnnnnnnnn nnnnnnnnnn
     6841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6901 nnnnnnnnnn nnnnnnnnnn nnctaaacca tttctttgaa gtcgttaccc tgcaaaaagg
     6961 tatcagtaaa attgtactcg atttttcctt ctctattatc agtattgttt tcgggttaat
     7021 cattttgcct ttatacaata cctggttttt tgttcttaca cttttacttg gagcgttttt
     7081 ctacttcatc attcggtatt atggtaaagc tggaattgaa accaatattc aaacttccaa
     7141 tcagaaatac cgcctcatgg atttgtttca acaggataaa cctcttgaat catcggacca
     7201 atctgaattc ctttcttgca tcgacgaaac attaggcgct tatttcaaag aacgaaaagg
     7261 tcattatgga gtgctcgaaa aacaatataa aggcatcgta ttttttaaag tgctttttat
     7321 tggaattctc ttgtttctag gagctttgct agttcaacag ggtaaattaa atattggtca
     7381 gtttgtcgct accgaaatca tcatattctt agtgattaat tccatcgaga aattaattgt
     7441 tagtttaaat agcttttacg acattattac agccctatat aaactggaga aaatagttgc
     7501 tgactcgccc gaatattctc tgtttagcaa gaagaaattc catttaccta gtttcgaatc
     7561 catttacatt cgtccgtact ctagggcaat taaatatttt atttatgcta tttgtctttc
     7621 agggcttatt ttactgtgta tgccttggac gcaaaacatc gagtcaactg gtacggttag
     7681 tactttgaat ccagctagtc gtccgcaaga agtaacttct cgtatttcgg gtagggtaga
     7741 aaagtggttt attcgggatg gtgatcgggt gaagaaaaac gatacaatcg cttatatatc
     7801 ggagattaaa gaagaatata tcgatccaga attagtctct cgttccgaat cacaaattcg
     7861 ttcgaaagaa tctgctatgg aatcgtacga gcagaaaata aatgcagtca atgctcaaat
     7921 tgatgccttg aatcgatcgc ttcggatgaa attggagcag gctagaaata agattgtcca
     7981 aggtaaaatc aaggttgtat cggatagtat tgaagcacat actgctcaag ctaatttcaa
     8041 agtagctgaa acacaattaa aacgttacga ggaattattg agcaaagggg tgatttcgcg
     8101 caccgacatg gaaaatcgtc gcataaaact gcaagaagcc ttggcaaaga aaacctctgc
     8161 tgaaaataaa ttcttcggta ctaaaaatga attactcaac accgaaattg agtttaattc
     8221 cattcaacaa gaatatgccg aaaagttgat gaaagccgat tctgagaaat actcggtgat
     8281 gtcggctttg tttgaagcag aaggatcact taccaagttg caaaatcaat tggttaatta
     8341 cagcatgcga caagggtttt attatgtgct agcaccacaa gatggttaca ttcaatccac
     8401 tgcaatcaaa ggaattggag agattgtaaa ggaaggagca tccttatgca gtatcattcc
     8461 tgatcaatcg gaacaagtag ttgaattgta tgtggaacct atgaatttgc cattaattcg
     8521 aaaaggacag catgtacaat taacgtttga tggctggcct gcatttgtgt tctctggatg
     8581 gccaggaatg agttacggaa cgtatactgc cgaaattgta gcatttgacc gatctatttc
     8641 agcaaatgga aaattcaggg ttctagctaa aagcattggc gagccttggc ctgatgctgt
     8701 tcggattggt agtggagttc aaggatttgc tttacttgga aatgtgccac tgttttatga
     8761 aatgtggcga caaatcaatg gattcccgcc tgaattctat actcaatcaa caaaaaagga
     8821 ccaagatgtt aaaaagtaaa ttttgctttt catttctttg ggttttgttg ctttttccct
     8881 cgagtgaagt tgtttctcaa gtgcttatca gttatgagaa gtttatggat gatgtaatga
     8941 aatttcatcc attatcggat cgtgcagaga atttcaaaaa gctcggaatg tatcagttgc
     9001 aagccgctcg tggttcttat gatccttttt tggaaggaaa tcacgagaat aaattctaca
     9061 acggaacaaa ttattattcg gtctcatccg ctcaacttaa acaagctctt ttcacttcac
     9121 aatatctcaa agcaggatat gattatggct atggaaatta tataaatcca gagttgaaaa
     9181 catctactgc aggcattcca tatttaggaa tggaagtctc cttattacaa ggattagtta
     9241 tagataaacg tagagcagaa gtgcttaaat cgcgtgctta tcttaattat tacacagcag
     9301 aacaaaagaa tcaattaaac cagttattct ttgaatcatc gcagcagtat ttcgactggt
     9361 tattcagctt aaaacaaatt tctttaaatg cttatttctt gcaattagcg caacaacggt
     9421 ataccggaat tgagtcacta gccgaagtag gggaaaggcc tgctgttgat actgttgaag
     9481 catctttatt tattcaatct cgtttgttgg atttgcaaaa tgctgaattg gataaaatta
     9541 aaagtcagaa tcaattagca tcatttaatt ggaatgaaaa tggaaatgct tctcttcaat
     9601 attcctttat accttcagat agcttggatg cctattttga aaaagcaaaa caagttatcg
     9661 gaaagctcat gttccaggat tcattaagca atccgatcat tagtaaatac actgcttttc
     9721 aagaagtttt gaatgtggat aaacggctga aacgcgaatt tatcaagcct aaattgaatg
     9781 taaactacaa cttacttgct gggaatacca acgcttctag tccaattttt acaaccaata
     9841 gctataaatg gggacttagt ttttcgatgc cattgctgtt acgtactcct gttaatgaat
     9901 accggatggc tgatatttcg agtaagaata attcattgga attgtcgagc aaaaacaatg
     9961 agttgatgta taagctaaat caagcttctc aatcaatttc tgtattatct cagcagttat
    10021 taaatgctca aaaatcggtc gattacagca gaaaattagt agatgctgaa aaacagaaat
    10081 tcgaggctgg tgaaagttct ctatttttga tcaatacacg cgaatccaaa tggcttgaat
    10141 cggaattgaa gcttgccgaa tacaagctaa aatttattaa atcgactcta caaattatct
    10201 atttgaaagg taatttgaat tatgtgtttt gatttcagtt aatacgcctt tgtccaatga
    10261 aaatgctcat tcactatttg tgttttattt tcttttagat gatttaaaaa ggcttgagct
    10321 acatagctgt gttttttacc ttttaaccaa atcaaattcc aactggtttt tattggaagt
    10381 ccttttacag gcagaatcga taaatcgccg ttcattagtt cattcttaat acctattagc
    10441 ggcattatcg aacaacctaa tccagctaaa attgcttgct taactgcttc atttgaggtt
    10501 agttcaagct tttttcgaac gtataattga tttttttgga agaactgttc catcgattgg
    10561 cgagttcctg aaccaagttc gcggtaaatc aaggcaagtt cttctaagta attcggtgat
    10621 ttcggttttg agcttgggga ataatcttta tttccaacaa gaaatagttt attctccatt
    10681 aattctagct tttctgtttg gattgattcg ggtaaaatgg agactaatgc taaatcggtt
    10741 tcattccttt cgagcttttc tagcactttt actttattcg atacatctaa attcaactca
    10801 attcccgggt gttttttaaa gaaatcactt agaatatacg gcatgatata tttgcctgta
    10861 gaaacaattg aaattgtaag ttttccactt agaaagcctt tgtatgctag tgttttgtaa
    10921 ttaattgcat atacttcttg gaggattttt tcggctgctt gtgctatctc atttccaaat
    10981 tcggtcacgt ataatttcct gccaataatc tctgtaagcg gaatttcaaa ttgatcttgg
    11041 aaattcttta attgtataga tacagcaggt tgtgtcagat gtaattcctc tgctgcttta
    11101 gtaatactaa gcgtttgagt tacttttaag aatacctgta attggtgaag tgtatactgc
    11161 attgattcga atttatattc ttaacattta ttgtttataa tagttcatcc gtgtatacta
    11221 ttttaaggat tacaataatt gcaaaacatc ggatcagctt tttcattcga tgttactaaa
    11281 tcaacttctt gattgaatcc acaagttaaa caggcatata tttttgattt tatttgatta
    11341 cttggcattc cacagtgtga acatgctaaa cctgtttcta ctcgagtgat aacaaaacca
    11401 ggcgttttac aacttggaca taagctattt atcttcttta ataattcctc acaagccaat
    11461 tgaatagatt ccattcgtgt tggattgtgc atagctctca tatctgtttc tatacgtact
    11521 tttccagtac gaatattcat ctgcctaaag agtttcctaa ctttcatttc atcatgaagt
    11581 cctttgtata tctcagattc cgatttcaaa attattccat gactagggaa atgaacttga
    11641 tttaaaaaac tacttaattc actttcggat tttatttctt cagaggcaaa attagttttt
    11701 gtatgaatga ttcgagcata tatttcccat ttatttttat cgtctattag tacaattatt
    11761 tcctcattag ctggaataaa gagatagctt ggatgcggac caaatgaacc ttcactggct
    11821 atacctaaat cacagttagt caatttcatt gcagccaagc atttttctcg cgctgtattc
    11881 agtggtgaat tatttcgttc aatttcgcca ctaaatgttc cataaacatc cgaattaaaa
    11941 ccatccggga gaaagcattg cacctgtaaa tgttgttcca atataggggc aataactttc
    12001 tccttatgat gcattgttgc aatcactaac ggacggtttt taaacgcaga cattattttc
    12061 ggacaatatt caactcggac tgaagttcca ctttaccatt cgatttttct atctcttttc
    12121 ggatatcata caaatctttt tgtaaagcct gaatgcgttt ttctctcatt tttatgtcct
    12181 cttcattcaa cagatgattt attttattct catgaaacct tattttcaca tgaatcatct
    12241 cggttattaa ttgcaaggca tcttgagagc taaaatgacc cgaaatcaac tgctttttca
    12301 tggctttttt attgtccttg tcctgcagaa taggctgttt ttccatcaaa ttcatataga
    12361 ttctcggttt taattgtgtc gatatttttc ttgaatgcgt gttgaagtat atcaatgata
    12421 gattctttac taatactcgc agattcattg gaaacaaagc gacatctcca atgatcggta
    12481 cagaaagttt ctttgaatcc ttcaggccaa acttttattc ctcgattggt tatcatcgat
    12541 aaatgcaatt cctgattatt taattccgaa atttttccag ctaattcatc cggattactt
    12601 cctccccaat gcacaaatac atctactcct ttcaattctt taagtgcaga tttttttctc
    12661 gcataaacgg gtaattcaag ttttccggtt gatttgtatt caactggctg aaaagtcttt
    12721 ggtttttgtc caagattctt tataacagcc aaggcaaatt catgtgtgcc aactagttcg
    12781 ctgcttactc cttccttaaa gatgtcatag gtatgaatgc catcttctaa ggttttaagc
    12841 caagcatttt gaattttctc agctatttca ttttgaccaa tgtgatttaa taggagaata
    12901 gcgccttgca acaatcccga tgggttagct ttgttttgtc cagctcttct cggtgctgaa
    12961 ccatgaatag cttcaaacat ggcacattct taacctatat tagatgagcc tgctaaacct
    13021 accgaaccag ctatttgtgc agcaacatcc gataatacat caccatataa attaggcatc
    13081 acaatcacat caaatagttc aggtgtatca gccattttag ctgctccgat atcaattatc
    13141 caatgctcat tttcaatttc aggatattcg gctgctattt cattaaatac cttgtgaaac
    13201 aaaccatctg tctgcttcat gatattgtct ttcgtgaagc aagtcacctt tttacgcttg
    13261 aaatgtttcg catactcaaa ggcataacga attatttttt cacatcccgg gcgactaatt
    13321 aacttcaagc attgaacaac ttcatccgtt tgttgatgct caattccggc atataaatct
    13381 tcttcattct ctcgtatgat taccaaatcc attttaggat gtttggtttc aacgaaagga
    13441 tgtaaactca tacaaggacg aacattcgca tatagtccaa ggaatttacg tgttgttaca
    13501 tttaagcttt tgtagccgcc gccttgaggt gttgtgatag gagcttttaa gaagatttta
    13561 ttttttcgga taatatccca ggattctttt gaaataccgg aagtgtttcc attgagatac
    13621 actttttctc ctacttcaat ttcatcccat tcgattttag ctcctgcagc taataagata
    13681 ttgagggttg catccatgat ctctggacca attccatctc ctttggcaat cgtaattcgc
    13741 gtcatcatgc tgattaattt ttcagcaaaa ctcagcactt gctttcatta atttttattt
    13801 tttgatttta tagttaggat aaataatatt tatacatcga gagctttgtc ctgttattcc
    13861 gcgaattcat ttcgaaaata cttgaaatct cttgcttaaa attccttggt tttgagaata
    13921 tagttctata aaatacatcc cattatttat gtttgggagt tcaattgaga aggaagattc
    13981 cccttttaat attttggatg aaatcaattt tccatcaatc gaataaatca tccatttctg
    14041 aatttttata gattgatcta attcaatttt tagttgattt tgagcaggtt gtggataaag
    14101 tttaatgggg tcatacgaac tagtggatgt gttaaacact aatggaaatt cttctcttac
    14161 agccaaaggt acataggcaa tattctcttt taatagcaaa tacaagggaa tattaaatcc
    14221 tcgatcacct aaagtattaa atacaccact attccaaatt gcccaaatgg catcaaacca
    14281 attcgaatga ttttcatttt cgtaacgggc gattttccaa caagctagta agagcgcatg
    14341 tggtgtgtca acccagcccg tttgattcgt ttccattact ccacgctgca aaatctccgt
    14401 agctaaatta tctgtcacca acaaagcata aggcttatac tgagctgcta atgctatatc
    14461 accacgatca cgaaaagcta cataggcttc agttaatgcc caagctatca tgctttgata
    14521 ccatggttgt gcattatgtg catcccaatt ccgacctggt gtttgcgctg atgcatataa
    14581 actgctaaat gcaatagtgg tattcatatt atccaccatt ccattttgat cccaatccgt
    14641 caatataccc ggacataaat ttcgatctaa acggtcgatt aaagcagctt taaaatcgga
    14701 tctacctgtc caggcgtaca attgcgctaa agcccacacg agttttgctg tgtaattatg
    14761 gttggttact aaaggatgtt gaatacacca ttctgcacct aaaacggctg catcacgata
    14821 acgttgtttg acaatcggat cggtttgttg aatcgcattt tctactaaaa aagcaacagc
    14881 ccaaccttga tcgtatcctg cgccaccaat atcgccgagg atgtttccat cttggataat
    14941 atgatcattt acgacgaatg tttcaccact atataatcca gcattgtaag gaatacaata
    15001 ggatggaaca gcgttgcaaa attggtcaac actagtgcca accacaccgc ttagctgaca
    15061 aggatcacct gttagtccat ggtaatatcc aaacattccc gcataggtag caagtggttc
    15121 aggagcgtgt tgtgttgaat attgttgggc ttcaagcatg ttgcccattt gttcgagtga
    15181 cgtgagattg ctgtaatatc ctgccattcg tgcatacagg gcagcaaaac catagcgtgg
    15241 cattcctcga gctggttcgg gacaagctgt tacatatgct tctaatgttg gtcgatatcc
    15301 cgaattccac acattggcaa tgctgctaag tgttggttcg gtgatattgg gtgcattaaa
    15361 tggaccatat tctacttgtg ggcaacctaa ggatattcca cgcgtttctt tggcttgttg
    15421 aatggccgtt gcaacatcac ttacagttcc agtaaacatt tgctgttgaa tgttagctcg
    15481 atttacatcg ctaccataat cgttaccaca gggcgcagtt acctgagcat tgagcaaata
    15541 agcttgttgc aataacagga aacaaagaat gtgcttcacc ggatttaatt tgatgcaatt
    15601 taagaattag gccgattgat tcagtttaat tgaaggactt taaacaggtt aagccaaatg
    15661 aattgcgaat ggatttgata aaaaaaggag ctttctcata ttccatccag ccagtccaga
    15721 atagtgaata ataaggacaa attgcaatat gattgtttaa gcgaaatgta taaccagctt
    15781 ttaatcgaat tccaaaattg gcaaaataac tataacgact atatatttta tttaaatcat
    15841 aattggttgg tggtttaaag ataaatgaat tatgaacgta acctgaaaat tgctcattta
    15901 tcaagcgaga atactcgaat ccaacttgaa agcgaacatt ttttcttatt attcgaaatg
    15961 ggtaaagtac aattccgagt gttgatttag taatatctac ttgagtagat gtgccacctg
    16021 ctaaaccact atgaattgct gaaaaatttg tgttgtattg attaaaatta atggcaaaat
    16081 cgagccgcaa ccaatcaagt attgcactgt caataccaag ttctattgaa tatccaggtg
    16141 ttgttttatt acttgtgaat ctgtcagaag aattttgatt gctgatatag ggatgattaa
    16201 aaatagttgc aatacttaaa ttaatattcc ggctttttga aatctgagaa aagcatggaa
    16261 aagctaatgt taaagcagtt aataaaaata gcaagattat tctattttgc attatagtat
    16321 tttttgattg caattaataa ttttaatgag atggttcaat gtgaattaac acatgaccta
    16381 attgcggaat tgtatttcgt aaatgatctt ttaattgatg cgctaaagta tgtccttcat
    16441 tgacacttat tttgccattt accatggcat gtaattccac atgatactgc ataccagctt
    16501 tacgaatgaa acatttttct gttcctaata tcccatttac ttcaatcgcg tgtatgcgga
    16561 tttctttaat taaatcatca tttaaatgtt catccataat ctcacctaat gcaggacgaa
    16621 atattttata actattccat aggataaaac cggatgcaaa caaggctgcc caatcatcgg
    16681 ccgattcgta gcctttgccc atgattaaag caatagagat gcctataaat gcagcaatgg
    16741 aagtgattgc gtcgctacga tgatgccagg catctgcttt aagtgaacta ctattagttt
    16801 ctcgactttt acgaattact aattggtacg aaatttcttt ccagagaatt attcctccta
    16861 aaatccaaag tgtgtaaggc ttgggtaatt catgcggagt gccaatattt gcaacacttt
    16921 cgtgtgcaat ataaaaagca gaactaatta agaacccaac aactaaaaag gtaattaaag
    16981 gttcaatacg accatgtcca tatggatgat tttcatcagc aggccgagcc gcatatttaa
    17041 gtccaagaag cactaatatg gaagcgaaaa tgtctgtagt tgattcaatc gcatctgcaa
    17101 caagtgcata cgaattacca aaatatcctc cagcaccttt tattaaggcc atagctgtat
    17161 ttcctgcaat actaaaccag gttgtgcgta cagcggtttc ttgatgcgac attagttgga
    17221 ttgaatcgca tgcaagttaa ggaagaatta gtcctgcttc tttgtaaaga tgagtattgc
    17281 ggtgagcaaa gctgtaattg ctgtcaatgg cgtcataatc catttttcgg tgctattaag
    17341 cgaaagcaaa ttcccaaggg tatttaaaaa aaacataatc ataattaccc atagaccaat
    17401 tctaacggct ttaggagata tattccaggc tagaatttga gcttttttca gaattattac
    17461 taaaaaaata atattcgaaa ttatagagaa tgtctcgaac tgaatgagtt gctcacgatt
    17521 ttcaattctt ccaccccaaa ccatctcata aggaattaca ttacctaaaa ctagaaagtg
    17581 aaataggaga gaagcgctga aaagaaaaat taaaatacca gatgcaattc gtgttagata
    17641 catagttttt agagttttat ctcaacaata gtatattacc ttgtttagta gtttcatccg
    17701 tattgctaaa tttatatttc aataaataaa tgtaattacc atcaggacaa tcttcttgtt
    17761 gatttttacc attccagtat tcatttgaat cggttgattt atatacaaca acgccccaac
    17821 gattatatat cgatatttca aatagttcaa attggcaaag agattttatt tctagaaatt
    17881 cattttttcc atcattattc ggagtaatta cattacttat ttcgaaggaa catttttctg
    17941 gatctattgg attttgtttg gattgacacg aatcaatgtg aatagtacga attaatgtat
    18001 ctattcctga tgaagtgctt atataaacag ttatatgata atatccttta ttaaagaatg
    18061 tatggggggc tgtatttcca acgccatagt tattggttcc gctgctcgga tcatcaaaat
    18121 tccaatctac atattgacca gaaactggat ttgtgagtga aaattgaatc ataactcctt
    18181 gacaactatc tgtatattga atttcggaag attgtggtgc tagaataccg cttgggaatt
    18241 tctctaatag actatttccc aataggatat tcgatgtaaa tggaaacatt agtataagaa
    18301 aatataatga ttgaattcgc atgatatagc tcattaaata gtcaatttag aacattgaaa
    18361 gttagtgcaa tttaggaatt cattttggtt aatttcatga atatatttct ttacacttta
    18421 caatttttct gttttatgcc agcattggat tgatatacat ctagcatttg aggcgttaat
    18481 ttaatgccag tttactccga ttaatagatt aaatgctagc cctgtacgat tgcctgttgg
    18541 ataatttgaa aatccatatt gcattctccc ttgtatgtct actgacatct ttcctttttt
    18601 gtaaaattcg tatccagttc caactaaaac agatggacca aaataaaatt ttcgttctgt
    18661 ttcatctttg atatcgtaaa atgcaggagc atcgaatgtt aatccagcac ctcctaatac
    18721 ccaataacga ttgctaaacc aatactggta acttggaatt ataccctcaa accctcgatc
    18781 tctcgttctt cccatatgtt tttcttggta gatcgaaccc ggtaaacaaa gtaaaatagc
    18841 tgaatgatca cttagcattt taccaatttt gatatttgga aaggacaatc cgatttgttg
    18901 ttcattccca attgctttag agcttagttg aatacttgaa atgcctgttg acactccaaa
    18961 gaagaagcgt ctttgttgta attttgtttc atcgttttgt gcggaaacaa aaagctgaca
    19021 aataatgagt agtaagagaa tcgtgtgttt catatgattt gaataacacg acaaagttct
    19081 cgagatcgtt aggtataaat gttaccacat ggtaacttct atcattgagt aatttttttt
    19141 cgaattctac ttagactttg tgttgtaata cctaaatagg atgcaatatg cttttgtgaa
    19201 gttctaagaa tgatatcggg atgtttactt aacatttctc gataacgttg ctcagcggtt
    19261 ttaagtaata aatcgatttg ctgctgttgt ttatcgacaa atgttgattc agcagaaatg
    19321 agaaacaaca gttgccccat aggtgtttgt tttagtttct cgatattttc cgatttaatc
    19381 ttaagcattt cggaatcttc taaagagatt gtttctaaag gagaggcttg gtgtgtaatg
    19441 agcgacatat aatcggcaaa gaattgatcc tcatacatga tatcgaggca tacataattg
    19501 ttttctttcc aaacaaatac tcctccactt cctgaaagta tgaaatagcc cgctttttct
    19561 gctattccag cttgcttgat gatttcattt tttttgaaag caacaggctc acataattca
    19621 agaaactgtt cccatgcttg aattggagct ttaaaataag ggtcaaatga cgattttaat
    19681 cgtgctgcta acatattaat tcagttaaaa tttgttatac ctcaaaaatt tacatcgatc
    19741 ttttatttta ttcatttccg atttaagtat ttattttttc caaatctgaa tatcatccac
    19801 ccatatttcc atgtccgaat catgtgtatt ggctgttatg ccgaattcta tactgctata
    19861 catacctttt gttccttgtt gaaaatacag tatatctttt ggtagcgttt tccaattaga
    19921 ttgatcaata attaaaacat catcttgcca aacttttaca taaccttttt tcttttgaga
    19981 taatttggtt tcaaacttta tgtgaaccca ttgatttcgt ggcatcttaa ctggtgttga
    20041 actcaattgc aaaatatccg gattattgta tttgtgttcc actctaattg cgccattaac
    20101 caattctaaa cgcattcccg gcccagctcc aatagctgtt tgttcttcta aatcgagcag
    20161 gaaaagccaa ttagcggaat catttcctgc taagtaatac catccttcaa cgaccaaaat
    20221 ctcattttcc caaaatgcca tgttttgttt agcaatactg gcttttgatg ctccgtgttc
    20281 gttggtagat ttggaagctt tgaaatgcat gctcttacta ccagtatgcg caaaactaga
    20341 atctacttct attaaattcc ctgaataagt attctgtgta aacgaccatt taacatcatt
    20401 atcaaatagc atttcatcga gtgtttgata actctcgaaa tcatcattga acgaatctgg
    20461 tccatcaaat aatttttctt tattacagga tgaaccaagt aaaatgagaa gaatacctaa
    20521 tagctttttc atttcttact cgaaagttga aatccgtatg taattcccgc ccaatgctgc
    20581 cattgaaaat cgatgatata agatacaatt ggagtatatg caatccgcca ataatgtcgc
    20641 ttattctcag gttgaaaacg at
//
LOCUS       DAOOQW010000024        20250 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_3242_length_20250_cov_5.195940, whole genome shotgun sequence.
ACCESSION   DAOOQW010000024 DAOOQW010000000
VERSION     DAOOQW010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 20250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20250
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3242_length_20250_cov_5.195940"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(85..864)
                     /locus_tag="PK281_02895"
     CDS             complement(85..864)
                     /locus_tag="PK281_02895"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HOY48319.1"
                     /translation="MKHTEVPSWISRMQLMTGETPILKLMNSHVLIAGLGGVGGICAE
                     MLVRGGIGKITIIDSDVVDETNRNRQIPALKSTEGRLKTEVMAERLLDINPDLDLHIH
                     SVYLREGVTEELIDTMKIDYAVDCIDTLSPKVFYLKKCLDRGIPVVSSMGAGGKIDPT
                     QLRVGDISESKHCNLARYVRKRLKALGIYKGIKVVYSPELANPDLIIKAPEGNVKKSF
                     IGTMSYMPAVFGCTVASVVLRDIGGLEEYRPTPARVPKKKK"
     gene            complement(861..1514)
                     /locus_tag="PK281_02900"
     CDS             complement(861..1514)
                     /locus_tag="PK281_02900"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOY48320.1"
                     /translation="MHFLNLHSHRKPQLTDEKVIRNAFVLDRSKLAPLPYFLSCGIHP
                     WLINIHWIDQLLKLENNLQNISQVIAVGECGLDRIKAADWKLQLEVFDAHIQLANKYQ
                     KPLILHLVKSYSDILAYSAHIQVPWIIHGFKGNRLEAEQLIKKGARFSFGPRLMLEKN
                     IQETFCNIPTEFIYLETDTKPIDIVAQYTYAAQLRNMPVEMLKEVIWTNFARDFKTS"
     gene            complement(1692..4073)
                     /locus_tag="PK281_02905"
     CDS             complement(1692..4073)
                     /locus_tag="PK281_02905"
                     /inference="COORDINATES: protein motif:HMM:NF039936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HOY48321.1"
                     /translation="MKKHLLFFLLICIKSVYAQNPNFSFVKSIGGTGNELGYATTVDA
                     SGNILTAGLFSGTTDFDPGPGTTNLTSIEYGDGFITKMDANGNFLWAYQIGGLGNDYI
                     NGIATDANGNVYATGQTGTSDLLIYKLNSSGNIQWSMTFGSPGGTEGGNGIEVDDLGN
                     VYVVGECMGTIDFDRGAGIANLVSAGSSDVFVAKYDADGNYIWAKRMGGIDSEYGKSI
                     AIDANFNVFTTGYWSNTADFDPGAGTFNMTTNGSPNDAFISKLDASGNFVFAKQFAGA
                     SHTYSHCIDLDPAGNIYTTGYFGGTIDFDPGAGTYNLSTTLGGDIFVSKLDGNGNFVW
                     AKAIIDNTTGNDIGNSIVVDDAQNVYFTGVFAGTADFDPGVGVFNITASAITDAYACK
                     LDINGNFGWAISWGGSSGDEARSIEIDNNQNLYFTGYFVNTVDFDPGTGVTNVVSNNA
                     SADAYLLKLSDCTLLNAPSAISGNTAICSASSNTYSVPAVSGATSYTWTLPSGWSGTS
                     TTNSITTTSNSTSGDITVSANNSCGPSAATTLAVSVETQPSAPGAITGPANLCESSNQ
                     TYSIVSVSGATNYVWNLPNGWTGTSNSISIDALAGLTGGNITVQAENACGISSSQSFT
                     VVVNPLPAVSLNLSPNLLCVNTSATALNGGLPAGGIFSGNGINATEFEPSLAGLGTHT
                     ITYTYTDSNSCISYATDNIVVDACLGIKEMINVNEIELFPSPTSGEFTIQCDEFSNHL
                     GTITIYNILGEVIYSNQTQSSQIHIDISQFSNGIYYAKIEIGNRIRVIELMKY"
     gene            complement(4528..4918)
                     /gene="ssrA"
                     /locus_tag="PK281_02910"
     tmRNA           complement(4528..4918)
                     /gene="ssrA"
                     /locus_tag="PK281_02910"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            5032..6231
                     /locus_tag="PK281_02915"
     CDS             5032..6231
                     /locus_tag="PK281_02915"
                     /inference="COORDINATES: protein motif:HMM:NF017072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOY48322.1"
                     /translation="MRTIGLIREGKKPYDKRVALTPTQCNELIERFPDVRILVQPSDH
                     RCIADAEYAAAGIELREDLSECDILLGIKEVPIDELISGKTYLFFSHTIKKQPHNRKL
                     LRKILELNIKMVDYETLVWENGSRVLGFGRYAGIVGTHEGFLTWGKKMKQFDLKPAWL
                     CRDYNEMMEQYKHINLPPIRIALTGDGRVAHGCLQVLKALNIREVTPNEFLHDSFDVP
                     TYVHLVTEDFYVHKDGDEWDKADFYQNPENYVSAFRPYTKSCDLMLNAIFWNERIPRF
                     FSREDMKARDFRIKVIADISCDVNGSVPATVKDTTIEDPTFGFHPLSETVEAPYLPNT
                     IDVMAVSNLPCELPLDASQGFGEHLLKYVIGGLLQADGHPMIKRATIAENGKLTERFS
                     YLKDYVE"
     gene            complement(6219..9782)
                     /locus_tag="PK281_02920"
     CDS             complement(6219..9782)
                     /locus_tag="PK281_02920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOY48323.1"
                     /translation="MNRILLKSSPNGIITASLEQMGDRLFFLQLKGQLDSSFQSSMQS
                     IFRYLNENRIADQLVLDIGHVSVSNSTFSPALYRQFKSALNASGLKTIALVNAPAWFN
                     RILDLTRNLRNQTKISFHRDKHSALQLFFREEIETKENTVNSVDYLLDQLFPHSYNRP
                     EILETKENPEWNYKSDSDDFECRFYHLNDDVLLGYVRGELNESNADIFLACKKKAIAE
                     MSHKPFYAIYHLSGITRVNKATRRKMEDHDREIRSYYAHCYWIVGGTTRSIVTMYRLF
                     SPGKFQHVTTVKSVSEGLVCIEESKKRLGITGTGHKIQPIQEDEIQKLKAEIQQLKSV
                     QQTRINQLVEYVSNDQNNKTIPVEIPEDDPFSVLFSGVENLQKSIHEELDQAQTNNSS
                     LKNKVDERSEEVIIKESNLRAILDNTDDEIYLLNERYELIDYNTNFENNFYARYGVFV
                     EKGRELFSMMPPEYGGLIRKSKERIDKALQGFQRTYYDKFQLGFYESISEIKLYPIRS
                     SNRSISGVSIFVRDCTEQKRSEDIIHQNQLLLSSINRNIKEGLYRSTPSRGMIYVNQA
                     FVEMFGFASEEQAIQAPSKSLYADANRRAELVQLIEQNGSFTNEEVRFCKKDGSYFWG
                     LLSSMRSIDTEGNVYYDGAIRDITHIKEYEREIIHSKEIAENATRAKSDFLATMSHEI
                     RTPMNGVIGMTSLLADTPLSPEQRDYVETIKVSGDHLLNIINDILDFSKIEAGHLELE
                     NTDFDLNSCIEEVMNLFSSRAYEKNIELFYRVENSEVFQLIGDVTRFRQVIVNLIGNA
                     IKFTNEGEVVIDVKPIEKKKGELRLRISVSDTGIGIPAEKQEKLFKPFSQVDNSTTRK
                     YGGTGLGLAISKHLVELMGGTLTMNSEVDKGTTFIFEVEMQESKQEIKKEHNLDVLQG
                     KRVLIVDDNMTNRKILEQLFKTNGMVVESYNNPLIALNIIKQGKQFDLGLIDMKMPEM
                     DGISFGKELLQIKTERELPLILYSSIGHMLSRTDINKYFKAHVNKPIRHDLLLQKMSS
                     ILGEQPFIQAKEEIKEIAEEQVASLYPMRVLLAEDNMINQKLAERVLEIFGYSISIAN
                     NGAEAVEMLQENGYDLIFMDVMMPEMDGLEATQTIRKSYTGKQPVIIAMTANALKGDR
                     EICIESGMNDYVSKPINTEEIRNLLVKYGSLIKSGAEVYST"
     gene            complement(10046..10693)
                     /locus_tag="PK281_02925"
     CDS             complement(10046..10693)
                     /locus_tag="PK281_02925"
                     /inference="COORDINATES: protein motif:HMM:NF020463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HOY48324.1"
                     /translation="MIVSDQPILLQLNYTPLVDQMALIATGIPAQIESQENYQKQSFR
                     NRCHVLTSQGVLPLVIPVLHNKGEAIRYTEAQTDSKNPWAVQHWRSLVSSYNKSAYFE
                     YYKHHFEAIYLQPNQNLYAFNYSLLGIFCRIFQFELPNENKIWEVVPTDTTDLRSLFQ
                     PKKINSLVSSPYYQTFEMPEGFVANLSCYDLLFNLGPESLSYLKKRGIELQHQIG"
     gene            complement(10690..11751)
                     /gene="lepB"
                     /locus_tag="PK281_02930"
     CDS             complement(10690..11751)
                     /gene="lepB"
                     /locus_tag="PK281_02930"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOY48325.1"
                     /translation="MKNLRKHLKEWGKAFLWAFLVAWFVRTLLIQGAFIPSQSMERTL
                     LPGDFVFINKWSYGPRMPITPFAIPFMHQNMPFSNSTPAYLDWVALPYWRIPGFGDIE
                     RQDVVVFNYPMEQERPVDKRTYFVKRCVALPGDSLRIDAKQVFINGKPQNNLPDFQFL
                     RRIKCSTALDQQWLDSLDITEGGLVSNMLDYEFPLTDSIAKVLEKHPFVHSMSMRMEA
                     SGDYQAYIFPHSRNYLWNNDHWGPVQVPFEGQVITLNDTNIVLYERIIREYEHNELSV
                     TGGKFYINNQAVTTYTIQQDYYFMMGDNRDYSADSRSWGFVPRSHVIGKAWLIFFSYN
                     ASVEGKSHIRWNRIFRFIE"
     gene            11912..12517
                     /locus_tag="PK281_02935"
     CDS             11912..12517
                     /locus_tag="PK281_02935"
                     /inference="COORDINATES: protein motif:HMM:NF017966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromophore lyase CpcT/CpeT"
                     /protein_id="HOY48326.1"
                     /translation="MKSFIITLLLILPMLTNAQFSKDHKRLASLMQGSYSSEEQHKAD
                     SAFFDIRLKIVPIWTSRTDALWMYVEQAVAGKEDRPYRQRVYKITETGGGNFESAVYT
                     LSAPLRFVGHAELVEKINPDSLQLRDGCSVLLKKINKKKFVGSTDNKKCPSDMRNAAY
                     ATSVVSIGPKLLESWDRGYDKDDRQVWGAEKGGYLFRKIKR"
     gene            complement(12507..13301)
                     /locus_tag="PK281_02940"
     CDS             complement(12507..13301)
                     /locus_tag="PK281_02940"
                     /inference="COORDINATES: protein motif:HMM:NF024852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HOY48327.1"
                     /translation="MSILGGGWLGFPLALDLIKQNFSIRISSTTKDKLKHFRQIGLQA
                     FDIELDTRKSIQPEFWSEVLVICIPPGRYGNYRAYTSKLQDLLRQMPADTVKQIILIS
                     STSVYPKISGNWNEKAVYDSESEPATCILIAEQLVMNSHPTVVILRMAGLAGYDRNPY
                     LYGTGKFAGNEPVNMVHRDDAIAVIKHFIEHKNFRGIFNVCAPEHPLRRDYYAHGAHA
                     VKQAAPQLPNSENPVLRVINSDKLIELTGYTFLKPSPVHFWDEPSV"
     gene            complement(13307..14281)
                     /gene="rluF"
                     /locus_tag="PK281_02945"
     CDS             complement(13307..14281)
                     /gene="rluF"
                     /locus_tag="PK281_02945"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: protein motif:HMM:NF007784.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HOY48328.1"
                     /translation="MNESNETRLNKYISESGLCSRRQADTYIEKGHVLINGRRAHVGD
                     KVKPGDRVMVNGQEIESRATEEEILIAFNKPIGVVCTTELAVKNNIIDYLKFGQRIFP
                     IGRLDKDSQGLIFLTSNGDIVNKILRAGNQHEKEYLVTVNKPVTDAFVEGMSKGVPIL
                     GVNTRKCFVSKESAIVFRIILIQGLNRQIRRMCEHFGYEVTRLERIRIMNVSLKNLGI
                     GTWRDLTKVEREGIQEMISRSSMEPEKKKSNPTPKSADKRKATSTEKSRTTAHSKEKS
                     SRTRKSSSSTSTTGASTSGKKRNNPGHKPYFPGAAGSKKSSGGRRGRR"
     gene            complement(14340..14885)
                     /locus_tag="PK281_02950"
     CDS             complement(14340..14885)
                     /locus_tag="PK281_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48329.1"
                     /translation="MIKLTNFFSPIRILLVLTLFISSIIQGAETKVKLKFNTQNYSFF
                     ISDLLLVKDMNVTDISAKIGNTKHVRERKNGEQAFFYDDLGITVLAINGKLRGVTVTL
                     NSDGDKNYVEKSYTGEFNFGKVSVSSKTVKSDFKKLDGFFVCGMPGLCGSKNKKAAIK
                     VLAGFQTSGLSKITYLSFLFN"
     gene            complement(14961..15863)
                     /locus_tag="PK281_02955"
     CDS             complement(14961..15863)
                     /locus_tag="PK281_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010519014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HOY48330.1"
                     /translation="MFKIAWHKSYPLPLPSGHRFPMEKYSLIPEQLIYEGIAKTENFF
                     EPHLIHDEILALVHEKNYLHKLNTCSLSASEIRKIGFPLSPELIEREKRLTGGTVEAA
                     FFALEFGIAFNGAGGTHHAYADRGEGFCLLNDQAVAAAYLLDQKKVNRIAIIDLDVHQ
                     GNGTASIFQDEPRVFTFSMHGASNFPLHKEVSDWDIPLPDGTTDEVYLSTLQSSLLHI
                     IESFKPEFIFYQSGVDVLKQDKLGRLGLTEAGCKKRDQLVFQAAHSNHIPVCVSLGEG
                     YSERISDIVNAHVNTFRCAQEIWF"
     gene            15937..17811
                     /locus_tag="PK281_02960"
     CDS             15937..17811
                     /locus_tag="PK281_02960"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOY48331.1"
                     /translation="MQNRYPTRFFKLTQFLLSLTALIVVQFDLDAQSSSSKSFFRTYG
                     LTTSDYGTWVCETSDQGYLLSFNSPYNSLVAGGLVKTDCSGEIIWQHLYSANNENILT
                     KQVEEINGSFYVYGAAGNTLNNAQLYFARLSADGTLQQAKYIRCSPSDNPARFLHASD
                     GSWWLISTGDNNVDYENICVTHLDYNLNFISSKKYSLPQKELIVESAALTTDGELVLC
                     GDYSSGSTFRNAYVMRIGVGGELRWFSTISHSYDVFLNDVVTDAAGFSYACGYMFDNV
                     SGWDAIMVKFNTTGDLSGQVQIDIAEDDRFRSLAFRDANLLLLGDFGTFDDRNIFWLK
                     INTTNSSVGDPVQLVWGDPYTNYPYKVVAGSAGSWLFTGDFAEPSGIRNAGLVRLDTY
                     YQLPCNTLAIADFNHEVGTFTDGIDNPSIQIIQAQVTDASFTDIPLIYSTTSVCGVYS
                     PSAAASFELQEDCPLSCAQFTDQSLCNPTSWSWEFPDGDPLVSSDPNPTVCYNSQGQK
                     TVYLTVSNADGSSSTSFSVDVKKDCPLIIPNTFSPNGDGINDLFTIIGLKSNSTFYVY
                     DRWGKKVFASTAYSNNWDGKIQDSSGSASNELISAGVYFYALEAPDGSKNNGYIQVVR
                     "
     gene            complement(17821..18345)
                     /locus_tag="PK281_02965"
     CDS             complement(17821..18345)
                     /locus_tag="PK281_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48332.1"
                     /translation="MMSSRRFIHYVFSLFCFGFSLLADAQIDTIKYEHYRSAKNCIYG
                     EIAGSGYLLSVHYERTLWMNESLSFNARIGVGSALFINAIPLVGLNATYGKKASKLEV
                     GFNAIRTYTFGIMGGESTYLMGNPILGYRYQKANGFLFRATVCPFFPLYDPDNWLQSR
                     LFVPYGGLSFGYAF"
     gene            complement(18354..19988)
                     /locus_tag="PK281_02970"
     CDS             complement(18354..19988)
                     /locus_tag="PK281_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014200746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HOY48333.1"
                     /translation="MSESIEANRNEDTMKLLLSSMRQKLEKVMLGGGKKRIDKEHEKG
                     KMTARERIQYLLDKGAEQLEIGAFVGDGMYEEHGGCPSGGVVVVIGYIKGRQCIVVAN
                     DATVKAGAWFPITAKKNLRAQEISIENRLPIIYLVDSAGVYLPMQDEIFPDKEHFGRI
                     FRNNAVMSSMGILQISAVMGSCVAGGAYLPIMSDEAMIVKETGTIFLAGSYLVKAAIG
                     EDIDNETLGGATTHCEISGVTDYKCENDADCLDRIRNIMDKVGAPAQAGFDRSESKLP
                     KKEVKELYSILPETREKPYDIHDIIDRLVDDSDFEEYKEKYGQSIVCGLGRIDGWAVG
                     IVANQRKVVKSKKGEMQFGGVIYSDSADKAARFIMNCNQKKIPLVFLQDVTGFMVGSR
                     SEQGGIIKDGAKMVNAMANSVVPKFTVVIGNSYGAGNYAMCGKAYDPRLMLAWPTAKI
                     AVMGGAQAAKVLLQIEVSSLKAKGKLITPEDEKELYDRIFNHYEATTSIYYSAARLVI
                     DAIIDPIDTRKAISMGIEAANHAPIEKRYNVGVLQV"
     gene            complement(20013..>20250)
                     /locus_tag="PK281_02975"
     CDS             complement(20013..>20250)
                     /locus_tag="PK281_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HOY48334.1"
                     /translation="ERASTSLKLTAQDMLKNKLIDGIIPEPSGGAHTNYHEVFSTVKA
                     ELKKHLDKLTKMKPEKRIEKRIEKFSGMGAFIEE"
BASE COUNT         5919 a         4202 c         3695 g         6434 t
ORIGIN      
        1 atgcgtgaat ttaaagacgc aaaagacggt aaaaccgaac ttcctgaagc caacaaagaa
       61 aaacaaactc agattaattc ctgattactt tttcttctta ggaacgcgtg ctggtgtagg
      121 acgatattct tctaaaccac caatatcgcg taatactaca ctggcaaccg tacagccaaa
      181 tactgctggc atgtacgaca tcgtgccaat gaaactcttt tttacattgc cctctggagc
      241 tttaataatt aaatctggat tggctagctc aggtgaataa accaccttga tgcctttgta
      301 aatacccaaa gccttcaagc gcttacgaac gtatcgtgct aaattgcaat gtttcgactc
      361 tgaaatatcg cccactcgta attgggtagg atcaattttt ccaccagctc ccattgaact
      421 cactactgga atacctcgat cgagacattt cttgaggtaa aatactttgg gagatagtgt
      481 atcaatacaa tcaactgcat aatcaatctt cattgtatca atcaattcct cggtaactcc
      541 ttcgcgcaga tatacgctat gaatatgcaa atctaagtcg gggttgatat ccagcagacg
      601 ctcagccatc acttccgttt tcaatcggcc ttcggtactt tttaatgctg gaatctgtcg
      661 gttacgattg gtttcgtcta ccacatccga atcaataatg gtgatttttc caatgccacc
      721 gcggactaac atttcagcac atatcccacc aacgccacct agtccagcta taagcacatg
      781 actattcatc aacttcaaaa taggtgtttc accggtcatt aactgcatcc ggctaatcca
      841 gcttggtact tcagtgtgct tcatgaggtt ttaaaatcgc gtgcgaaatt agtccaaatg
      901 acttctttta gcatctctac tggcatattt ctcaattgag cagcatatgt gtattgcgca
      961 actatatcga taggcttcgt gtctgtttcg aggtagataa attcggttgg tatgttgcag
     1021 aatgtttctt gaatgttttt ttcaagcatt aagcgtggtc caaaagaaaa gcgtgctcct
     1081 tttttgatga gttgttcggc ttctaaacga ttgcctttaa aaccatgaat aatccagggg
     1141 acttgaatgt gagcggagta ggctaaaatg tcggaatacg atttcaccaa atgtaaaatg
     1201 agcggtttct gatatttatt ggctaattga atgtgtgcat caaaaacttc taattgcagt
     1261 ttccagtcag cagctttaat tcgatccaat ccacattcac ccacagctat aacctgactt
     1321 atgttttgca aattattttc gagcttaaga agctgatcta tccaatggat attgattagc
     1381 caaggatgaa ttccacaact taaaaaatag ggcagaggag ctagtttcga ccgatccaaa
     1441 acaaaggcat tccgaatgac tttttcatca gtcaattgcg gtttacgatg agaatggagg
     1501 ttgaggaaat gcatgaggat tttgtgtaat gaatacaatg ataattaata aaaaaggaca
     1561 aactcatttt gggaatttgt ccttaaaagc tcaaatatta tgaggtttag cttagtaatt
     1621 taattatttc aaaattatag aaatgcgggt atgaatcaca tacagctgat ttatttctaa
     1681 aataaaatga attaatattt cattagttca atcactctaa tccgattccc aatttcaatt
     1741 tttgcataat aaataccgtt tgaaaactga ctgatatcta tatgaatttg tgatgattgg
     1801 gtttgattcg aataaatcac ttcacccagt atattgtata ttgttattgt tcctaaatga
     1861 ttagaaaact catcacattg gattgtaaat tcacctgaag taggagatgg gaacaactct
     1921 atttcattca cgttaatcat ttcttttatt cctaaacatg catccacgac aatattgtct
     1981 gttgcataac taatacacga atttgaatcg gtgtaagtgt aggtgatagt atgtgttcct
     2041 aagcctgcca aggatggttc aaattcagtt gcatttattc cgtttccaga aaatattcct
     2101 ccagcgggta aaccaccatt caatgctgtt gcagaagtat ttacacacaa taaattagga
     2161 cttaaattca agcttacagc aggtaaagga tttactacta cagtaaatga ctgactactt
     2221 gaaattccgc aggcattttc agcttgtaca gtgatatttc ctcccgttag tccagctaat
     2281 gcatcaatac taatactatt agaagtgcct gtccatccat tgggtagatt ccaaacatag
     2341 ttagttgccc cagaaacaga aacaatgcta taggtttgat tgcttgactc acaaagattt
     2401 gcaggtccag taattgctcc cggtgcagaa ggttgagttt caactgaaac ggctaaggtt
     2461 gttgctgcac ttggtccgca agaattgttc gcgctaactg taatatcacc actcgtggaa
     2521 tttgatgttg ttgttatact attggttgta gatgtgcctg accaaccaga aggtagtgtc
     2581 caagtataag atgttgctcc tgatacagct ggaacagaat aggtgtttga actagctgag
     2641 caaattgctg tattacccga aattgctgat ggagcgttaa gtagggtaca atcagataat
     2701 ttcaacaaat aagcatctgc agaagcatta ttggatacta cattagttac acctgtacct
     2761 gggtcaaaat ctactgtatt cacaaagtag cctgtgaaat atagattttg gttattgtca
     2821 atttcaatac ttcgtgcttc atcaccactg gatcctcccc aacttatagc ccatccaaaa
     2881 tttccattga tatccaattt acaagcatag gcatccgtaa tcgctgaagc agtgatatta
     2941 aatactccta cacctggatc aaaatctgca gttccagcaa aaactcctgt gaagtataca
     3001 ttctgtgcat catccacaac aattgaattc cctatgtcgt tgcctgttgt attatcaatg
     3061 attgcttttg cccaaacaaa attcccgttt ccatcaagtt tgcttacgaa gatgtcgcca
     3121 cccaaagtcg ttgacaaatt atacgtacca gcacctggat caaaatcgat agttccacca
     3181 aaatagcccg tcgtgtatat gtttcctgct ggatctaaat caatacaatg gctataggta
     3241 tgactagcac ctgcaaattg tttggcgaag acaaaatttc cagatgcatc tagtttacta
     3301 ataaatgcat cgtttggact cccattcgtg gtcatgttaa atgtacctgc acccggatca
     3361 aaatccgcgg tattgctcca atagcccgtt gtaaatacat taaaattggc atcaatagcg
     3421 attgactttc catattccga atcgataccg cccattcttt tcgcccatat atagtttcca
     3481 tcggcatcgt attttgctac aaacacatcg gaagaaccag ccgatactaa attggcaatg
     3541 cctgctcctc gatcaaaatc gatggttccc atgcattctc ctaccacata tacattccct
     3601 aaatcatcta cctcaattcc atttcctcct tcagtaccgc ccggagatcc gaaagtcatt
     3661 gaccattgta tatttccaga tgaattgagc ttataaatta gtaagtcgct tgtgcctgtt
     3721 tgccctgtag cgtatacatt tccgttcgca tcagtggcaa ttccatttat gtaatcattc
     3781 cctaatccac caatttgata ggcccacagg aaatttccat tcgcatccat tttggtaata
     3841 aatccgtcgc catactcaat agatgttaaa ttggtagttc ctggtcctgg atcaaaatcc
     3901 gtagttccac taaacaaacc agctgtcaat atatttccgc tagcatctac tgtagtagca
     3961 tagcctaatt catttccagt tcccccaata ctttttacaa atgagaaatt aggattttga
     4021 gcataaactg attttataca aattaaaaga aagaatagaa ggtgtttttt cattcctata
     4081 tgcgtgtttg ggttaggaga gccaagaaat aattcaaagt ataaattaat tgcatgtccc
     4141 atatagtgag atgactaatg caactttttt gaacatttct taagtccaaa cttcaagctt
     4201 tttgctcaaa attcaaaatg gtataattca attgtaatct ttgcgacgaa ttatctaact
     4261 ccctaattga atcaagtgct tgatctagtt ctaaacgttg ataagggttt catgattaaa
     4321 ttaaaaaggg acaaactcct cgattggaat ttgtcccttc gggtggagcc ggcgggagtt
     4381 tcaaagaagt tcccttgcct tactcattgc aaatgcccga ttaaaatcgt tacacgattt
     4441 ccatccaaca gcacaaacgc aaaactcaag caagcttgat tttggtctga actgttgaat
     4501 gttcctgcgg aataatcagg cattcattcg tggagccggc gggagtcgaa cccgcgtcca
     4561 aacaaggaat tcacacgctt tctacatgct tatttccgaa tcaattgtcg gaagtgggaa
     4621 ggaacggaac aaaccagccc tcttcttagt cccgatcgtt ttcggtaatt catcaggacc
     4681 ctgaactacc tatctcaaac tttacgatgc ctcatcgggg acgacttgag aaaacttccc
     4741 cgggagacaa ctagtgttta cctataaggc aaacggggtt aatcctatcg gattaagctg
     4801 ctagtgcgta gttgttttcg ccagttatag gcgcgcggat tgttttaacg ggttaacctc
     4861 gcaacacccg gcatgcttac ttgctaattg cactcgctgt cgataccatg acggccccat
     4921 gatgtaaaga actcccgcaa agatacatca gtttttagcc ttttctgctt ctgtttcgtg
     4981 cttttcagta aaaggacttc ttcctttgaa agggcttact aactttgtcg catgagaact
     5041 atcggcttaa ttcgcgaagg caagaaacca tacgacaaac gagtagcact tactcccact
     5101 caatgtaacg agctaatcga acgtttccct gatgttcgaa tcttggtgca accctctgat
     5161 catcgctgta ttgccgatgc tgaatatgct gctgcgggta ttgaattgcg cgaagatctt
     5221 tccgagtgcg acatactttt aggcatcaaa gaagtgccaa ttgatgaact catctcgggg
     5281 aaaacatacc tcttcttttc tcataccatc aagaaacaac cacataatcg caaactcttg
     5341 cgtaaaattc ttgaactgaa cattaaaatg gtcgattacg aaaccctcgt ttgggaaaat
     5401 ggtagtcgag tgctcggatt tggtcgttat gcaggaatag ttggaacaca cgaaggtttc
     5461 ctcacttggg gaaagaaaat gaagcagttc gacttaaaac ctgcttggct ttgtcgcgat
     5521 tataacgaga tgatggagca atacaaacac atcaatcttc ctccaattcg tattgcttta
     5581 acaggtgacg gacgagttgc gcatggttgt ttgcaagtac tcaaagcctt gaatattcgt
     5641 gaagttactc caaatgaatt tttacacgat tcatttgatg ttcccacata tgtgcattta
     5701 gttactgagg acttttatgt tcacaaagac ggcgacgaat gggataaagc cgatttctac
     5761 caaaatccag agaactatgt ttccgctttt cgtccttaca ctaaaagttg cgatttaatg
     5821 ctcaatgcca tcttttggaa cgaacgcatt ccgcgctttt tctcgcgaga ggatatgaaa
     5881 gcccgcgact ttcgtattaa agtaattgcc gatatcagct gcgatgtaaa tggttctgtt
     5941 ccagcaactg tcaaagacac caccatcgaa gatcctacat ttggttttca cccattatca
     6001 gaaacggttg aagctcctta tttgcccaac accatcgatg taatggctgt tagtaatcta
     6061 ccttgtgaac tcccgctcga tgcttcccaa ggatttggtg agcatttgct caaatatgta
     6121 attggaggat tattgcaggc cgatggtcat ccgatgatca aacgagctac aattgctgaa
     6181 aacggaaaat taacggaacg cttttcttat ctgaaggatt acgtcgaata aacttcagca
     6241 cccgatttaa tcagtgaacc atatttcacg agtaaattcc tgatttcctc tgtgttaatg
     6301 ggcttactca cataatcatt catgcccgat tcgatacaaa tttctcggtc gcctttgagt
     6361 gcattggcgg tcatggcaat aatcacaggt tgcttgcctg tatatgattt gcggatggtt
     6421 tgagtggctt ccaatccgtc catttcgggc atcatcacat ccataaagat gagatcgtaa
     6481 ccgttttctt gtaacatttc aactgcctca gcaccgttat tcgcgataga aatcgaatac
     6541 ccaaaaatct ctaatacacg ttcagcgagt ttctgattga tcatgttgtc ttcggctaac
     6601 aaaacccgca ttggatataa gctagcaact tgttcttcgg caatttcttt gatttcttct
     6661 tttgcttgaa taaatggttg ctcgcctaaa atggaactca ttttctgcaa gagcaaatcg
     6721 tgacgaattg gtttattgac gtgcgctttg aaatatttat ttatatcggt tcgcgaaagc
     6781 atgtgtccaa tcgacgaata caagatgagc ggtaattcgc gttcggtttt tatttgaagc
     6841 agctcttttc caaacgaaat tccatccatt tcgggcattt tcatgtcgat caagcccaaa
     6901 tcaaattgtt ttccttgttt gatgatattg agtgcaatga gtggattatt atacgattca
     6961 accaccattc cgtttgtctt gaacaactgt tctagaattt tacggttggt catattatca
     7021 tctacaatca gcacacgttt gccttgcaaa acatcaagat tatgctcttt tttaatttct
     7081 tgttttgatt cctgcatttc cacttcaaaa ataaaagtgg tgcctttatc cacttcacta
     7141 ttcatagtaa gtgttccgcc catcaactcg accaaatgct tactgatcgc gagtcctaat
     7201 cctgttccac catatttcct agtggttgaa ttatctactt gagaaaatgg tttgaataat
     7261 ttctcttgct tttctgccgg aattcctatt ccagtatcac ttaccgaaat tcttaatcgt
     7321 aattctcctt ttttcttttc aattggcttc acatcaatca ctacttcacc ttcattggta
     7381 aacttgattg catttccaat taaattcaca attacctggc ggaaacgagt aacatctcca
     7441 attaattgaa atacttccga attttctact cgataaaata attcaatatt cttctcgtaa
     7501 gctctgctac taaagagatt catcacctct tcaatacaag aatttaaatc aaaatcggta
     7561 ttttctaatt ctaaatgacc agcttcaatt ttactgaaat cgagaatgtc gttgatgata
     7621 tttaataagt gatcacccga aactttaatt gtttctacgt aatcgcgctg ttccggactt
     7681 aatggtgtat cggccaacaa acttgtcatc ccaattactc cattcatcgg tgtccgaatt
     7741 tcatggctca tcgtagctaa gaaatcggat tttgctcgag tggcattttc tgctatttct
     7801 ttagagtgaa taatttcacg ttcgtattcc ttgatgtgag tgatatcacg tatggcaccg
     7861 tcataataaa cgtttccttc agtatctatt gaacgcatag aggataataa tccccagaaa
     7921 taacttccat cttttttaca aaaacggact tcctcatttg tgaatgaacc attttgctca
     7981 atcaattgaa cgagttcagc acgacgattt gcatcggcat ataaactttt cgatggagct
     8041 tgaatggcct gttcttcgga agcaaaacca aacatttcaa cgaatgcctg attcacgtat
     8101 atcataccac gagacggtgt actacgataa agtccttctt taatattccg attaatggaa
     8161 gataatagaa gttgattttg gtggataata tcttcggaac gtttttgttc ggtacaatcg
     8221 cgcacgaaaa tcgaaacacc cgaaatactt ctattggaag aacgaatggg atataattta
     8281 atttccgaaa tagattcgta aaaaccgagt tggaatttat cgtaatatgt tcgctgaaat
     8341 ccttgcaaag ccttgtcaat tcgctctttc gatttacgaa ttaatccacc atattcgggt
     8401 ggcatcatgc taaataattc acgtcctttt tctacaaaaa caccataacg tgcgtagaaa
     8461 ttattctcga aattggtatt gtagtcaatt aattcgtagc gctcatttaa cagataaatc
     8521 tcatcatcgg tattgtctaa aatcgcacgt aaattcgact ctttaataat tacttcttcg
     8581 gatcgctcat caactttatt cttaagtgat gaattatttg tttgagcttg atctaattct
     8641 tcgtgaattg atttttgaag attttctaca cctgagaaaa gcaccgaaaa tggatcatct
     8701 tcaggtattt ctaccggaat tgttttatta ttctgatcat tagaaacata ttcaactaat
     8761 tgatttatcc gagtttgttg aacggatttt aattgttgaa tttcggcttt taatttttga
     8821 atctcatctt cctgtatcgg ttgtatttta tgtcccgttc cagtaattcc caatcgtttt
     8881 ttcgattcct caatacacac taatccttcg gaaacacttt ttactgtggt aacatgttga
     8941 aatttccctg gagaaaacaa acgatacatc gtaacaatag aacgagtggt tcctccaact
     9001 atccaataac aatgggcata atacgatctg atttctcgat catgatcttc cattttacgt
     9061 cgtgtagctt tatttacacg ggtaattcca cttaaatgat aaatggcgta aaaaggctta
     9121 tgactcatct cggcgatggc tttcttttta catgctaaga agatatctgc attggattca
     9181 tttaattcac cacgtacata tcctagtaaa acatcatcat ttagatggta gaaccgacat
     9241 tcaaaatcat ccgaatcgct tttataattc cattctggat tttcttttgt ttccagaatt
     9301 tctggacgat tatacgaatg cgggaaaagc tgatctaata aataatcaac cgaattaact
     9361 gtgttttctt tagtttctat ttcttcccta aaaaataatt gtagcgcaga atgtttatct
     9421 ctgtggaaac ttattttagt ttgatttctc agatttcgtg ttaaatccaa aattcgatta
     9481 aaccaagctg gtgcatttac taatgcgatt gttttcaagc ccgaagcatt caatgctgac
     9541 ttgaattgcc gatataatgc aggagagaac gtcgaatttg aaaccgaaac atgtccaata
     9601 tctaatacaa gttgatccgc aatccgattt tcattcaaat agcgaaagat cgattgcatg
     9661 ctcgattgaa atgaagaatc gagctgacct ttgagttgca aaaagaacaa acgatctccc
     9721 atctgctcca gtgatgcagt tataattcca ttcgggcttg atttgagcag aatcctgttc
     9781 atctcaaaac tcgatttgaa gaattagttg gcagaataga atacttcttt ttcacacagg
     9841 taaaatgaca gctatttgtg caattgtcct gtttatttag aatgaagtcc ttacagatgt
     9901 aagaaggtga tgaatttcaa ttataaatat tttctaaatc cttgaaatag ttggtaatta
     9961 tatattgagt attaacgcat gggggcaaat actcctaatt ctgcacgtaa gtttttttat
    10021 tgttggaggt aaaatccagt gagaattacc caatttgatg ctgcaattcg attcctcttt
    10081 tctttaagta agacaaagat tctggaccta aattgaaaag taaatcatag caactcaaat
    10141 tcgccacaaa tccttctggc atttcgaatg tttgataata gggagatgaa actaaagaat
    10201 tgattttttt gggttgaaat agagacctca aatcagttgt atcggttgga actacttccc
    10261 aaattttatt ttcattggga agttcaaatt gaaatatcct gcaaaaaatc cctaataagc
    10321 tataattaaa cgcatataga ttctgatttg gttgaagata tatcgcttca aaatgatgct
    10381 tgtaatactc gaaataagcc gatttattgt agctacttac taagcttctc caatgttgaa
    10441 cagcccaagg atttttagaa tcagtttgtg cttctgtata ccgaatggct tcacctttat
    10501 tgtgcaacac tggaatgacc aatggtaaaa ctccttgtga tgtaagcaca tggcaacgat
    10561 tgcgaaaact ctgcttttga tagttttcct gtgattcaat ttgtgccgga attcctgtgg
    10621 caattaaggc catttgatca accaaaggcg tgtaatttaa ttgcagtaaa atgggttgat
    10681 ctgaaactat cattcaataa atcggaatat acggttccat cggatatgag attttccttc
    10741 taccgaagca ttgtaggaaa agaatatcaa ccaagctttt ccaatcacgt gactacgtgg
    10801 gacaaatccc caagaccgcg aatcggccga atagtcgcga ttatcaccca tcataaaata
    10861 ataatcctgt tggatagtat atgtcgtaac cgcttgattg ttaatataaa actttcctcc
    10921 tgtaacagat aattcattat gctcatactc ccgaataatc cgctcgtaca atacaatatt
    10981 ggtgtcgttt aaggtgataa cttgtccttc aaatggtact tgaacaggtc cccaatgatc
    11041 attattccat aggtaattgc gactatgtgg aaagatatac gcctgataat cacctgatgc
    11101 ttccattcgc atcgacatag aatgaacgaa gggatgtttt tcgagcactt ttgcaatact
    11161 atcggtgagc ggaaattcat aatcaagcat attcgaaacc aagccacctt cggtaatatc
    11221 aagcgaatcg agccattgct gatcaagtgc agtgctgcat ttaattcttc ttaaaaattg
    11281 aaaatcgggg agattatttt gaggttttcc gttaataaaa acctgtttag catcaatcct
    11341 caaactatca cccggcaagg ctacgcagcg ttttacgaaa taggtgcgtt tatccaccgg
    11401 tcgttcttgt tccatgggat aattaaaaac gaccacatct tgacgttcaa tatctccaaa
    11461 acctggaata cgccaatagg gtagtgcaac ccaatcgaga taagccggag tggaattact
    11521 aaaaggcata ttttggtgca taaacggaat cgcaaatggt gtgataggca ttcgtggtcc
    11581 gtacgaccat ttgttgatga aaacaaaatc acccggcaat aaggtgcgct ccatgctctg
    11641 actcggaata aatgcacctt gaataagtag cgtccgaaca aaccaagcta ccagaaatgc
    11701 ccataaaaag gcttttcccc attctttcaa atgtttgcgt aaattcttca ttcgatacac
    11761 gaagatacgc gctacagctc gctaggttcg gctttatgcc actttctatc aacattagat
    11821 agctcaattc ctgatattaa ctgtctatta tttcttttca aaccgcattc agcgcttgta
    11881 cattcaatac ttttgcaaaa aatatcattt catgaagtcc ttcattatta ccttgttgct
    11941 gattcttcca atgcttacta atgctcagtt ttcgaaagat cataagcgtt tggcttcact
    12001 catgcaaggt tcatatagca gcgaagaaca acacaaagcg gattctgctt tttttgatat
    12061 ccgacttaaa attgttccca tttggacttc acgtaccgat gcattatgga tgtatgtaga
    12121 acaagctgtt gctgggaaag aagatcgtcc atatcgtcaa cgtgtctata aaatcacaga
    12181 aacgggtggt ggaaatttcg aaagtgccgt ttataccttg agtgctccat tgcgatttgt
    12241 tgggcatgct gaattggtgg agaaaatcaa tcccgattcg cttcaattac gcgatggatg
    12301 ttcggttttg ctgaagaaaa tcaacaagaa gaaatttgtt ggaagtacgg ataataaaaa
    12361 gtgtccttcg gatatgcgaa atgcagccta tgcaacatct gtagtttcaa tcggccctaa
    12421 gctattagaa agctgggatc gaggttacga caaagatgat cgtcaggttt ggggagccga
    12481 aaagggagga tatttatttc gtaaaattaa acgctaggtt catcccaaaa atgaactggg
    12541 ctgggcttta gaaaagtata accagttagt tcaattaatt tatcagaatt aattactctt
    12601 aaaacaggat tttctgaatt aggaagttga ggcgctgctt gcttaactgc atgagctcca
    12661 tgtgcataat aatctcgtcg gagcggatgt tcgggcgcgc acacattgaa tattcctcgg
    12721 aaatttttgt gctctataaa gtgctttatt acagcaatag catcatctcg gtgaaccata
    12781 tttacaggtt cgtttccagc aaattttcct gttccataga gataaggatt gcggtcgtag
    12841 cctgctaatc ctgccatacg taaaattacc acagtcgggt gactattcat cactaattgt
    12901 tcggcaatta aaatacaggt tgctggttct gattctgaat cgtaaacggc tttctcattc
    12961 caattgccac ttattttggg ataaaccgaa gtactgctaa ttagaataat ttgttttact
    13021 gtatcggcag gcatttgacg cagtaaatcc tggagtttgc tcgtatacgc acgataattt
    13081 ccatatctac ctggcggaat acaaatcact aaaacttcag accaaaattc gggttgaatt
    13141 gattttctag tatctaattc gatgtcgaaa gcttgtaatc cgatctgcct aaaatgcttc
    13201 agtttatctt tcgtagtgga agaaattcga atactgaaat tctgtttgat taagtcgagt
    13261 gctaatggaa atccaagcca tccgcctcct aaaatactca ctccattcat cgtcttcctc
    13321 gtctaccacc cgaacttttc ttacttcctg ccgcacctgg gaaataaggt ttgtgacccg
    13381 gattatttcg cttttttcca gaagtggatg cacctgtagt cgaagtagat gaactgcttt
    13441 ttcgggtgcg gctacttttt tccttggaat gagcagttgt gcgacttttt tcagttgatg
    13501 tagctttcct tttatcagct gatttgggag taggattact tttcttcttt tccggttcca
    13561 tgctcgaacg actgatcatt tcctgaattc cttcccgttc aacttttgtt aaatcgcgcc
    13621 aagttccaat tcccagattt ttcaaactca cgttcatgat gcgaatgcgc tctaaacgtg
    13681 ttacctcata accgaaatgc tcacacattc gacgaatctg tcggttcaag ccttgaatga
    13741 ggataatccg aaacactata gccgattctt tactcacaaa gcacttgcga gtattgacgc
    13801 ccagaatggg aactccttta ctcattcctt ccacaaaagc gtctgtaaca ggcttgttaa
    13861 cagtcaccaa atattctttc tcatgctgat tccccgcgcg tagaatttta ttcacgatat
    13921 caccattgct agtgaggaat atcaagcctt gagaatcttt atctaatcga ccaatgggga
    13981 aaatccgttg tccaaattta aggtaatcga tgatattatt ctttactgct aattcggttg
    14041 tacaaaccac gccaataggc ttgttgaatg caattagtat ttcttcttct gtggcacgcg
    14101 attcaatttc ttgtccgttt accatcaccc gatcacccgg cttaacttta tcacccacat
    14161 gcgctctgcg accattaatt agcacatggc ctttttcgat gtaggtatca gcttgccggc
    14221 gagaacaaag tccgctttcg ctgatgtatt tgtttagtcg tgtttcgttt gattcgttca
    14281 tgataaaatg aattcgattg actaaaccct tgaaaattca agcttagtag aagtgattgt
    14341 tagttgaata ggaaacttag ataggtgatt ttagataatc cagaagtttg aaatccagct
    14401 aaaactttga ttgctgcttt cttattcttc gaaccacata atccgggcat tccacacaca
    14461 aaaaaaccat ctagtttttt gaaatccgat ttcactgttt tggagctcac ggatactttt
    14521 ccaaaattga attccccagt gtaagacttc tctacatagt ttttatctcc atccgaattc
    14581 agcgttaccg tgacaccgcg caatttccca ttaatggcaa gtactgtaat tcctaagtca
    14641 tcatagaaaa atgcttgttc tccattttta cgttcacgca catgtttagt atttccaatt
    14701 ttagcggaaa tatcggtcac gttcatgtct ttaacgagta gtaaatctga aatgaagaag
    14761 ctataattct gcgtattgaa tttcagttta acctttgttt cagcaccttg aattatactc
    14821 gaaataaaca gggttaaaac aagcagaatt cggattgggc taaagaaatt agtgagcttt
    14881 atcatgtgcg caaatttcaa tgattttttc cagtatgaac atgcttttca attgaaaaat
    14941 atttcgtcca ccaattggta tcaaaaccag atttcttgtg cacaacgaaa ggtatttaca
    15001 tgtgcattca caatatccga aatccgttct gaataacctt cccctaaaga aacgcaaact
    15061 ggaatatgat tagaatgagc agcttgaaac accagttgat cacgcttttt acagcctgct
    15121 tcagtcaaac ctaaacgtcc aagtttatct tgttttagca catcaacacc gctttggtaa
    15181 aagataaatt caggtttgaa cgattcaatg atgtgcaaca aactactttg taacgtgctc
    15241 aaatagactt catctgttgt tccatcgggt aaaggaatgt cccaatcaga aacttctttg
    15301 tgtaagggga aattggaagc accatgcata ctgaatgtaa acacgcgtgg ttcatcttgg
    15361 aatatggaag ctgttccatt gccttgatgc acatctaagt cgataatggc aattcgattt
    15421 accttttttt gatccaataa gtaagcagct gccacggctt ggtcattcaa caagcaaaaa
    15481 ccttcacctc gatcagcata tgcatgatgt gttcctccgg caccattgaa tgctataccg
    15541 aattctaagg caaaaaatgc agcttcaacg gttcctcctg taagtcgttt ttcgcgttca
    15601 attaattcgg gtgagagtgg aaagccgatt ttacgaatct cacttgccga taatgaacag
    15661 gtgttaagct tatgtagata atttttttcg tgaactaagg ctaggatttc gtcatgaatt
    15721 agatgtggct cgaagaaatt ttcagttttt gcaatccctt cataaataag ctgttcggga
    15781 ataagagagt atttctccat cggaaatcta tgcccgctag gtaaaggaag tgggtagctt
    15841 ttatgccaag caatcttgaa catgtgcgta caaattcatt caaagtttga aaattgatgt
    15901 ttctcttgtt agatttatcc caaatcttcc aacgttttgc aaaatcgtta cccaaccaga
    15961 ttcttcaaat taactcaatt cctgcttagc ttaactgctt tgattgttgt ccaatttgat
    16021 ttggatgctc aaagctcatc ttctaaatcg tttttccgca cctatggatt aacaaccagc
    16081 gattatggaa cttgggtttg cgaaacttcc gatcagggtt atttgctttc ctttaattcg
    16141 ccctataact cattggtcgc aggcggatta gttaaaacgg attgctcagg ggagataatt
    16201 tggcaacatt tgtattcagc caataatgaa aatatcctca ctaagcaggt agaagaaatt
    16261 aatggtagct tttacgtata cggagctgca ggtaatacac tcaataacgc tcaattgtat
    16321 tttgcccgct taagtgccga tggaactttg caacaagcta agtatatccg ttgctcacct
    16381 tctgataatc ctgctcgctt tttacatgct tccgatggat cctggtggct catcagtact
    16441 ggcgacaata atgtggatta tgaaaatatc tgtgtaacgc atcttgatta caatttgaat
    16501 ttcatttcat ccaagaaata ctcacttcct caaaaagaac ttattgttga atcagcagct
    16561 ttaactaccg atggggaact tgttctctgt ggagattatt cttcaggttc tactttccga
    16621 aatgcttacg taatgcggat tggagtagga ggtgagcttc gatggttttc aactattagt
    16681 cattcgtacg atgtgttttt gaatgatgta gttaccgatg ccgctggttt cagttatgcg
    16741 tgtggttata tgttcgataa tgtttctggt tgggatgcca ttatggtgaa attcaacacc
    16801 acaggtgatt tgtcgggaca agttcagatt gatattgcag aagatgatcg ttttcgatca
    16861 ttagctttca gagatgcgaa tttgttattg ttaggtgatt tcggaacatt cgacgaccgc
    16921 aatattttct ggctgaaaat caatacgaca aattcttctg taggtgatcc tgttcaatta
    16981 gtatggggtg atccatacac gaattatcca tataaagtag tagcaggctc agctggaagc
    17041 tggttattta caggtgattt tgctgaacct tctggaattc gaaatgccgg tttagttcgc
    17101 ttggatacct actatcaact tccatgtaac accttagcca ttgccgattt taatcatgaa
    17161 gtaggaactt ttacagatgg aatcgacaat ccatctattc aaattattca agcccaagta
    17221 acagatgcca gctttacaga tattccatta atctattcaa ctacgagtgt ttgtggcgtt
    17281 tattcacctt ccgcagcagc cagcttcgaa ttacaggaag attgtccatt gagttgtgct
    17341 cagtttaccg accaaagttt atgtaatcct acatcctggt cttgggaatt tcccgatgga
    17401 gatccgcttg tttctagtga tcctaatcca acagtttgtt acaattctca aggacaaaaa
    17461 actgtctatc tcaccgttag taatgctgat ggtagcagtt caaccagctt tagtgtggat
    17521 gttaaaaagg attgtccgct gattattcct aatacattct ctcccaatgg agatggaata
    17581 aatgacctat ttacgattat tggtttgaaa tcgaatagta cgttttatgt gtatgatcga
    17641 tgggggaaaa aggtatttgc ttctacagcg tactccaata attgggacgg aaaaattcag
    17701 gattcatcag gatcagcatc aaatgaactt atttctgcag gtgtatattt ttatgcttta
    17761 gaagcaccag atggctcaaa aaataacggc tatattcagg ttgtacgttg agcaatcaat
    17821 ttaaaaagca tatccaaagc taagtcctcc ataaggcaca aataggcgac tttgtagcca
    17881 attatcagga tcgtaaagcg gaaaaaaagg acaaacagta gctcggaaca aaaatccatt
    17941 agctttttga taccgatagc caagtatggg attacccatg aggtaagtac tttcaccacc
    18001 cataattccg aaagtataag tacgaatggc attaaatcct acttctaact tactggcttt
    18061 ttttccatag gtagcattta atcctacaag tggaatggca ttgataaaaa gcgcagaacc
    18121 aactccaatt cgagcattga atgataaaga ttcattcatc caaagagttc gttcgtaatg
    18181 taccgatagc aggtatcctg atcctgcaat ctcaccataa atacaattct tggcactacg
    18241 ataatgctcg tattttatcg tgtcaatttg agcatctgcc aataacgaaa aaccaaaaca
    18301 aaacagactg aaaacataat gaatgaatcg tctgctggac atcataatca atattaaact
    18361 tgtaaaacgc ctacattgta acgtttctca ataggagcat ggttggcagc ctcaattccc
    18421 atcgaaatcg ctttacgtgt atctatcgga tcgataatcg catcaattac caaacgtgca
    18481 gcactgtaat agatggatgt tgttgcttcg tagtgattaa agatacggtc gtaaagttct
    18541 ttttcgtctt ctggagtgat taattttcct ttcgctttta aagaactcac ctcaatttgt
    18601 aacaatactt ttgctgcctg agctccaccc ataactgcaa tcttagctgt tggccaagct
    18661 aacatcaaac gtgggtcgta tgcttttcca cacatagcat aattgcccgc accataacta
    18721 tttccaatca cgacagtgaa tttcggtacc accgaattag ccatcgcatt caccatttta
    18781 gctccgtctt ttatgattcc accttgttca gaacgggagc cgaccatgaa gcctgttacg
    18841 tcctgcaaaa acaccaaggg aatcttcttt tgattgcaat tcataatgaa acgtgctgct
    18901 ttatcagccg aatcactgta aatcactcca ccaaactgca tttcaccttt tttactcttc
    18961 accactttcc gctgattggc aacaattcct actgcccagc catcaatacg tcctaatcca
    19021 caaacgatac tttgtccgta tttctctttg tattcttcaa aatcggaatc atcaaccaaa
    19081 cgatctatga tatcgtgaat atcgtaaggt ttttcgcggg tttctggaag gatactatat
    19141 agttccttaa cttctttctt aggtaattta gattctgagc gatcaaatcc agcttgagca
    19201 ggagcgccaa ctttatccat gatattccga atgcgatcta agcaatctgc atcgttttcg
    19261 catttataat cagtcacacc actaatctcg caatgtgttg ttgctcctcc taatgtttcg
    19321 ttatcgatat cttccccgat agctgcttta actaaataag aaccagccaa gaaaatagtt
    19381 ccagtttctt tcacaatcat ggcttcatca ctcatgattg gcaaataagc tccacctgca
    19441 acacaacttc ccatcaccgc cgaaatctgc aaaattccca ttgacgacat caccgcatta
    19501 ttccggaaaa tcctaccgaa atgttccttg tctgggaata tttcatcctg catcggtaaa
    19561 taaacacctg cactgtcgac aaggtaaatg ataggtaaac ggttctcgat ggatatttcc
    19621 tgagctcgta gatttttctt cgctgtaata ggaaaccaag ctcctgcttt aacagttgca
    19681 tcattagcaa ctacaataca ctgacggcct ttgatatagc caatcacaac cactacacca
    19741 ccactcggac aaccgccatg ttcttcatac atgccatcac ctacaaaagc accaatttca
    19801 agttgttccg ctcctttatc gagtagatat tggatgcgtt cacgagcagt cattttgccc
    19861 ttttcgtgtt ctttatcgat tcgctttttg ccgcctccta gcattacttt ttccaacttc
    19921 tgacgcatgg aagaaagtaa cagcttcatc gtgtcttcgt tgcgattagc ttcaatcgat
    19981 tcgctcatga aaatgggaac ttaatagtgg atttattctt caatgaaagc tcccattcca
    20041 gagaacttct caattcgttt ctcgatgcgt ttttcgggtt tcattttagt cagtttgtcg
    20101 agatgttttt tcaactctgc tttaactgta ctaaaaacct cgtgataatt ggtatgcgct
    20161 ccaccagatg gttctgggat gatgccatcg atgagtttgt ttttaagcat atcctgcgca
    20221 gttaacttaa gcgaggtaga tgcccgttct
//
LOCUS       DAOOQW010000025        20230 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_3245_length_20230_cov_5.533383, whole genome shotgun sequence.
ACCESSION   DAOOQW010000025 DAOOQW010000000
VERSION     DAOOQW010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 20230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20230
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3245_length_20230_cov_5.533383"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..694)
                     /locus_tag="PK281_02980"
     CDS             complement(<1..694)
                     /locus_tag="PK281_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48335.1"
                     /translation="MLKRYILAAFAVLSLHGIASAQCNPDPLYNDSTFGIWPDTITNL
                     PCAFADEAGGYNTVIDIKTLTDTTVSVTGFGNVTAYIEAFRVNGVTGLPTGFSYIPNA
                     ASWNNEGTVPDYISVQGCMSLLASQSAVQSIIDTNPNGADFPLVVTVDAKINSTDNTL
                     ANFVLAGKWLSQITTIPGIQAIPVSGYVVKVRPSSASGCLPLATIEIDNAAFEIQGNY
                     PNPFSNSTEIRFS"
     gene            complement(705..1076)
                     /locus_tag="PK281_02985"
     CDS             complement(705..1076)
                     /locus_tag="PK281_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOY48336.1"
                     /translation="MQFAHEKTEHYTILRVLAEKLDSTIAPDLKSDLLMLNKDGVVNI
                     ILDLTNTRYCDSSGLSSVLVGNRLCKNENGTLVLVGLQPSVRKLITISQLDPVLHIVP
                     TVSEAIDYLFMESIEKDLNNE"
     gene            complement(1162..2088)
                     /locus_tag="PK281_02990"
     CDS             complement(1162..2088)
                     /locus_tag="PK281_02990"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HOY48337.1"
                     /translation="MTMLSVRHLLGIKYLQKEDIELILHTADNFLDVINRPIKKVPSL
                     RDITIVNLFFENSTRTRLSFELAEKRLSADVINFSASSSSVSKGETLIDTVNNILAMK
                     VDMVVMRHPKAGAAHFLSKHVNAKIINAGDGAHEHPTQALLDAFSIRRKLGGIEGKKV
                     AIIGDILHSRVALSNIFCLQKMGAEVVVCGPPTLIPRDIQSLGVRVESDLKKTLEWCD
                     VANMLRIQLERQDIQYFPSLREYAMLYGLNKELLDNLNKEIVIMHPGPINRGVEITSD
                     VADSSHSIILQQVENGVAVRMAALYLLANRNE"
     gene            complement(2085..2621)
                     /gene="pyrR"
                     /locus_tag="PK281_02995"
     CDS             complement(2085..2621)
                     /gene="pyrR"
                     /locus_tag="PK281_02995"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF003549.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HOY48338.1"
                     /translation="MSHRIILDHQRFQLTLKRLCYQLIENHDKFENTVIIGLQPRGIL
                     LSRCIRNELGKLLGKSLIYGELDTTFFRDDFRIKPLVPASTSIDFVVEGKKVVLIDDV
                     LFTGRSIRAALDALQHFGRPANVELLVLVDRRLSRHVPVQPDYTGLTVDAIFSEQVVL
                     NWNSEETHAEVLLKNKES"
     gene            complement(2754..4646)
                     /gene="rpsA"
                     /locus_tag="PK281_03000"
     CDS             complement(2754..4646)
                     /gene="rpsA"
                     /locus_tag="PK281_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020023259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HOY48339.1"
                     /translation="MSEINQDTPETTAPAAVAETSKASKTPIESISSLDFDWDSVGKK
                     SSNYSKSERERMESLYEKSLSSITEHEVVDGVIVAMTSKDAVVNIGYKSDGIVALSEL
                     RYDPEIKVGDRLEVYIESKEDKSGQLLLSHKKARSLKSWDRVNAALENDEIIKGYVKC
                     RTKGGLIVDVFGIEAFLPGSQIDVKPIRDYDVYVGKSMEFKVVKINKEFKNVVVSHKA
                     LIEEELEAQKAEIISKLEKGQVLEGTVKNITSYGVFVDLGGVDGLIHITDLSWGRINH
                     PEEIVKLDEKIQVVILDFDEGKKRIALGLKQLTSHPWDKVDANLNVGDKVKGKVVVLA
                     DYGAFVEIEPGFEGLVHVSEMSWSQHLRSAQDFLKVGDEVECIILSLDREERKMSLGI
                     KQLMPDPWADIETKYPVNSKHSAKVRNFTNFGVFVELEEGVDGLIHISDLSWSKKIKH
                     PSEFTKIGEDIDVVVLDTDKENRRLSLGHKQLEENPWDVFETIFTEGSVHQGTITSMN
                     DKGGVVALPYGVEGFAPNKHLAKADGSVAKAEETLDFKVIEFAKDSKKIIVSHARTHE
                     EEKAGARDEKSETKESRADDTKKAMKKVKDSQEKSTLGDLDVLANLKSDLEQSEKKAA
                     AKKSKD"
     gene            complement(4806..6218)
                     /locus_tag="PK281_03005"
     CDS             complement(4806..6218)
                     /locus_tag="PK281_03005"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOY48340.1"
                     /translation="MKISQTSYARSLLKKFRKDHIYQEIPTTEVIERQRFVVFRIFSV
                     IAILVCISVASKMLLTLGMINWLPFTILLLGLIILLNYFSISKRDNLKRSYTIMMVSC
                     FVLLHLVSYTCGGIRTGGTFFMSAVIIYSFMLLGSRGGWIMSIACGIHIIYLFILATY
                     TDLTSFSLFQDRIDLINEDFLVNILLTFAMISALSSYLQSGRNVVIQSLVDAKNTLET
                     QNALLEKNNRALEKKNAELDKFASVASHDLRSPLRAIGSLTDMILEDDQSLCFDTHEK
                     LKIIHGRVNRMDHLLTALLEFSRADRKNQEISKVDIRALILGLLVKHSNGREINYELP
                     ANLPVIKTFPLKLEAVFSALIKNAIQFNHQEIPTIQIDLFSELGKLKFTVSDNGPGIH
                     ADFHEKIFVIFQTLEARDKFESTGAGLAIARKIVEENGGSIRLESNIGEGSRFIFTWE
                     AEEIISDSSTTWLHQLSKSA"
     gene            complement(6304..7191)
                     /locus_tag="PK281_03010"
     CDS             complement(6304..7191)
                     /locus_tag="PK281_03010"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF002187.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HOY48341.1"
                     /translation="MNVIIDANSGFCFGVVYAIQMAEDELNATGELYCLGDIVHNNKE
                     VDRLEKKGLRIIDHEELRELHNTKVLIRAHGEPPETYKIAIENNIELIDASCPVVLKL
                     QNRIRNGYEHLGDGQIVVYGKEGHAEVNGLVGQTNGKAIIANSIEDLDQIDFKRPIQF
                     FSQTTQPTEGFKAMRAEIIKRMQAEGQDENLFFEANDTLCRQVSNREPQLLEFAKAHD
                     VIVFVSGKKSSNGKVLYDVCKSVNPNTYFVSEWEEVESNWFKSTDSVGICGATSTPMW
                     LMEQIADQIRQIHQFDLID"
     gene            complement(7263..7961)
                     /gene="cmk"
                     /locus_tag="PK281_03015"
     CDS             complement(7263..7961)
                     /gene="cmk"
                     /locus_tag="PK281_03015"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HOY48342.1"
                     /translation="MPPRKITIAIDGYSSCGKSTIARALAARLHYNYVDTGAMYRAVT
                     LYALRNGLINGKQLDEEGLIDELDQVHIQFLFNPETQASEILLNDENVEQYIRQMDVN
                     DWVSQVSAVGEVRRKMVKIQQELGKGKGIAMDGRDIGTTVFPDAELKIFMTAETNIRT
                     QRRLDEMDSKGISANFEQVRDNLLQRDLEDMSRAESPLRKADDAIILDNSNLSREQQL
                     DFVLKLVDQLTVKS"
     gene            complement(7961..9004)
                     /gene="porQ"
                     /locus_tag="PK281_03020"
     CDS             complement(7961..9004)
                     /gene="porQ"
                     /locus_tag="PK281_03020"
                     /inference="COORDINATES: protein motif:HMM:NF033711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system protein PorQ"
                     /protein_id="HOY48343.1"
                     /translation="MNFRFLFLLIFLAQLDVSAQIGGQNVYSFLNLPNSARVQALGGS
                     VISLRDDDVNLAYQNPGLINSSMAGVTSLTYVKYFAGINFGNAAYAFNVKKAGTFALS
                     MQYIDYGNFERADATGQLNGEFTAGEYCLTTGFGRKIDSLFSVGVNVKTIISNLEAYS
                     SFGMALDLGASYLSPDKLFEASVLVKNVGYQFNSYVDGNRETLPFEAQIGLSKRLLHV
                     PLRFSFVAHNLQQPVMTYDDPLKPTETIDPLTGDTIINKIGVGNKILRHAILGAEFNI
                     TKGLSFRLGYNYQRRQEMKVDSRLGTVGLSWGLGVKIYKFSLAYARSAYHLAGSPNQI
                     TLSAKLSEFYRKH"
     gene            9154..10023
                     /locus_tag="PK281_03025"
     CDS             9154..10023
                     /locus_tag="PK281_03025"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HOY48344.1"
                     /translation="MQFDRKTAIENSELHTQEIQILELFDKNDKLTIFDIGACEGESS
                     IRYSRIFPNSKIYTFEPNPSNFKLVEKNIENFSSREQITPFQLCLTDKTGTAEFYISS
                     GTPEEFKEKEVDWDFGNKSSSLLPPDKTLEAFDWLKFNDVVQVKTERLDNLMKENNID
                     QIDFIHMDVQGAELMVLNGSGEKISRINNIWLEVEGIPLYKGQPIRKDVEKYMRNIGY
                     VKILDSFDKVAGDQFWSKKEWLLTKKSENWIKEREQKLKEEEKARQKPLIESIRDKVQ
                     LRSRIRSILGGKK"
     gene            10075..10968
                     /gene="fabD"
                     /locus_tag="PK281_03030"
     CDS             10075..10968
                     /gene="fabD"
                     /locus_tag="PK281_03030"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013550877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HOY48345.1"
                     /translation="MKALVFPGQGAQFTGMGKDLYDQFPVVRDLFEEANDILGFRLTD
                     LMFSGTDEELRRTDVTQPAIFLHSVAIARLMGDSFKADMVAGHSLGEFSALVANKTLS
                     FADGLKLVSKRASAMQVACEQNPGTMAAILGLDDSIVEEVCAGIVGEIVVPANYNCPG
                     QLVISGSLKGIELACEAMKAAGAKRALPLQVGGAFHSPLMEPARAELAAAIAATQFSI
                     PNCPVVQNATALPASDPAVIRENLIAQLTAPVRWTQSVRKMADDGVSQFVEAGPGKVL
                     QGLVKKIAPEATAASAELVQS"
     gene            complement(10959..11699)
                     /locus_tag="PK281_03035"
     CDS             complement(10959..11699)
                     /locus_tag="PK281_03035"
                     /inference="COORDINATES: protein motif:HMM:NF039343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphatase"
                     /protein_id="HOY48346.1"
                     /translation="MGWLSSIFGSAQAEADLGQLRTDIHSHLIPGIDDGATSMEDSLN
                     LIRGLHSLGYTKLITTPHIMSDFYKNTPEIILSGLEKVREAVKLEGIPVELHAAAEYY
                     CDSNFEKSIDDKTLLTFGEKYVLFELSYLNLTENFETVTFKLQMEGYIPVLAHPERYP
                     YWYQSFNKYEELRDKGILLQINLASITGHYGTVAKRISERLIEQNMVSFLGTDTHKMS
                     HVDVFRKARKEPALKKLIESGSLLNTQL"
     gene            complement(11701..12699)
                     /locus_tag="PK281_03040"
     CDS             complement(11701..12699)
                     /locus_tag="PK281_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003444430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOY48347.1"
                     /translation="MYQHVRFHSNEALFQQSVFLVTGGAGFIGSHIVDYLLEHGAAEV
                     RVVDNLSEGRIDNIRHWLQDARFKYFEGDICDQTLMEKAMNGVDYVSHQAALGSVPRS
                     IKTPQATNEANVTGFLSVIDHARRAGVKRMVYASSSSVYGDHPVLPKVESQIGNPLSP
                     YAVTKRVNELYADVFGRTYGLKIAGLRYFNIFGPRQKPDGPYAAVIPLFIQSIMDSSP
                     AYMNGDGGQTRDFTYVENAVQANIRALFSENEEATGKVYNVAVGERVSVLEMYDILRE
                     YAGKGLDPVHREEREGDIRDSLADITLAKTYLGYEPQVKMREGLRKTLEAFRVLSI"
     gene            complement(12714..13769)
                     /gene="rfbB"
                     /locus_tag="PK281_03045"
     CDS             complement(12714..13769)
                     /gene="rfbB"
                     /locus_tag="PK281_03045"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HOY48348.1"
                     /translation="MSKKLLITGGAGFIGSHVVRRFVTNYPDYEIYNLDKLTYAGNLM
                     NLKDIENLPNYHFVKGDIVDDAFITELFNLQQFDGVVHLAAESHVDRSISNPTEFIRT
                     NVLGTVNLLNAAKHIWQGNMEGKRFYHISTDEVYGSLGETGFFTEETKYDPRSPYSSS
                     KASSDHLVRAYFHTFDLPVVVSNCSNNYGSFHFPEKLIPLAINNIKHMRPIPVYGKGD
                     NIRDWLFVEDHARAIDLIFHKGRSGETYNIGGNNEWTNIDLIHKLCEIMDEKMNRAPG
                     LSASLITFVKDRAGHDHRYAIDFTKLKNELGWEPSLQFEEGLRRTVEWYLTNEEWLNA
                     VTSGDYQRYYNSQYEKR"
     gene            complement(13762..14805)
                     /gene="galE"
                     /locus_tag="PK281_03050"
     CDS             complement(13762..14805)
                     /gene="galE"
                     /locus_tag="PK281_03050"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020213535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HOY48349.1"
                     /translation="MNQSKPCVLVTGGMGYIGSHTVVALFRQGYDVVIADNLCNSDVL
                     ILDRIEQISAQRPRFHQLDLCDKTSVRELFKLEPTIQAVIHFAALKAVGDSVKKPLMY
                     YQNNLGSLLNLLETMQESGVNNIVFSSSCTVYGEPDSLPVTETTPVKPATSPYGNTKQ
                     IGEEILADLSKVSDVHSIALRYFNPVGAHESALIGEFPKGTPNNLVPFITQSAIGKLG
                     PLQVYGNDYNTPDGTCIRDYIHVEDVAEAHVAAMHRLLSNQQQDTFEVFNIGTGQGNT
                     VLEAIHAFEQSSGQKLQYIITSRREGDIEKVYADTSKSNQILGWKAEKSLFEMMQSAW
                     AWELQLSKNSQNE"
     gene            complement(14815..16113)
                     /locus_tag="PK281_03055"
     CDS             complement(14815..16113)
                     /locus_tag="PK281_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOY48350.1"
                     /translation="MSSIYQNLIEKKDSLAVVGLGYVGLPIALEFARNIQVVGFDINA
                     ERVEKMRNRIDPSEELAPEEFDGCEITFTADPDVLRKARFFIVAVPTPIDEHNLPDLK
                     PLLSATRSVAAALKKGDYVVYESTVYPGCTEEDCIPLLEQISGLKFNEDFKVGYSPER
                     INPGDREHTLSRIIKVVSGCDAESLDNIARTYEIVVKAGVHRAASIKVAEAAKIIENT
                     QRDLNIALMNELSMIFGQMGINTYDVLEAAGTKWNFLRFLPGLVGGHCIGVDPYYLTF
                     KAKEVGHNAQVILAGRSVNDGMGAYVAGKVVKKMVAQGVDIAKGRVLIMGATFKENVS
                     DIRNSKVADVVKEFVEFGVHVDIIDPHASSKDFLHEYGFPIASQASGIYDAIVVAVNH
                     SDYVGLPESYFTEMLTDKGIVADIKGVYRGKLNHVKYWSL"
     gene            complement(16110..17144)
                     /locus_tag="PK281_03060"
     CDS             complement(16110..17144)
                     /locus_tag="PK281_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOY48351.1"
                     /translation="MILNNKVKFAIIGAGHIGKRHAEMVLRNEDAELVAICDILPREK
                     LGLDNFDVPYFDSAEALFNSGLEFDVVNVCSPNGLHAQHSVLALENRKHIVCEKPMGL
                     SKAECENVIYTALRMSRQVFCVMQNRYSPPSVWVKDVIDRRLLGDVYMVQLNCYWNRD
                     DRYYKAGSWKGSPDLDGGTLFTQFSHFIDIMFWLFGDIKDIQAKFNDFNHQNSTAFED
                     SGFVTFNFHNGGMGALNYSTSVWDQNLESSITIIGEKGSIKIGGQYMNEVEYCHIQDY
                     EMPKLAAANPANDYGAYKGSAANHHYVIQNVIDTLKGRTSATTNALEGLKVVEIIERI
                     YELRTLKKLQ"
     gene            complement(17141..17719)
                     /locus_tag="PK281_03065"
     CDS             complement(17141..17719)
                     /locus_tag="PK281_03065"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014083590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOY48352.1"
                     /translation="MDYYAHPTAIIDEGCTIGKGTKIWHFTHVMSNCQLGENCNLGQN
                     VVISPQVVLGNNVKVQNNVSIYTGVSCDDDVFLGPSMVFTNVINPRSAVNRRDQYLKT
                     HVGRGASIGANATIVCGHDIGEFAFIGAGAVVTKTVPAYALVIGNPARQTGWMSTFGH
                     KLKFNDQGLAICPESGEQYKLEHGVVTNLGKP"
     assembly_gap    17769..17868
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(17880..18866)
                     /locus_tag="PK281_03070"
     CDS             complement(17880..18866)
                     /locus_tag="PK281_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOY48353.1"
                     /translation="MKYDIKGKRVLITGAAGFLGSHLCDRFMAEGCYVVGMDNLITGS
                     LQNIEHLFKEERFEFYHHDISKFVFVPGNIDYILHFASPASPIDYLKIPIQTLKVGSL
                     GTHNLLGLARVKGARMLIASTSEVYGDPTIHPQTEDYWGNVNPIGPRGVYDEAKRFQE
                     AITMAYHTFHGVETRIVRIFNTYGPRMRLNDGRALPAFMGQALRGEDITVFGDGSQTR
                     SFCYVDDLIEGIYRLLLSDYAYPVNVGNPDEISIRDFAEEIIKLTGTDQKIVYKPLPQ
                     DDPKQRQPDITKAREILNWEPRVPRSEGMKHTWDYFKALPEWVITPRSFTSA"
     gene            complement(18863..20185)
                     /locus_tag="PK281_03075"
     CDS             complement(18863..20185)
                     /locus_tag="PK281_03075"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009086967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HOY48354.1"
                     /translation="MKNIAVIGTGYVGLVTGTCLAETGNNVICVDIDKDKIAKLRKGT
                     VTIYEPQLDVVFERNLKQGRLHFTTDLKEAIDASELIFLALPTPPGEDGSADLSYILG
                     VSEDIGHLLTDSYKIIIDKSTVPVGTAEKVHKALAKNAKAEFDVVSNPEFLREGFAVE
                     DFMKPDRVVIGTSSSRARKVMEELYLPFVRQGNPILFMDERSAELTKYAANSFLATKI
                     SFMNEIANLCELLGADVDQVRMGIGSDDRIGKRFLFPGIGYGGSCFPKDVQALVKSSS
                     DVDYHFSILHSVMEVNEKQKVKLMPKINKFFNNDLKGKRIAIWGLAFKPDTDDIREAP
                     ALYIIDELLAAGAEVCAYDPEAMANVKKTGRKIEFAKGQYEAIEGADALVICTEWSAF
                     RTPDFERMISQIKYKVIFDGRNLYTIDQMKKLGLSYFSIGRKSVRKAK"
BASE COUNT         6200 a         4069 c         3825 g         6036 t
ORIGIN      
        1 aagagaaacg gatttcggta gaattagaga aagggttagg ataatttcct tgaatctcaa
       61 atgctgcatt atcaatttcg atggtagcaa gaggtagaca acctgaagca gaacttggac
      121 gaaccttaac tacatatcca gaaacaggaa tagcctgaat accaggtata gtagtaattt
      181 gagaaagcca tttaccagcc aatacgaaat tagctaatgt attatctgtt gaatttatct
      241 tcgcatcaac tgtaacaact aatggaaaat cggctccatt aggatttgtg tcgatgatgc
      301 tttgaacagc agattgacta gcaaggagcg acatacaacc ttgaacagaa atataatcag
      361 gtacagtgcc ttcattattc caagaagcag catttgggat gtaactaaag cctgtaggca
      421 aacctgttac accatttact ctaaaagcct ctatatatgc agttacatta ccgaagccag
      481 ttacgctaac tgttgtatct gttaatgttt taatatcaat aacagtgtta taaccaccag
      541 cttcgtcagc aaatgcacat ggaagattgg tgatcgtatc aggccagatt ccaaatgttg
      601 aatcgttata aagcggatcg ggattacact gagcagatgc gattccatgt agagaaagca
      661 ctgcaaaagc agctagaatg tagcgtttta gcatagattg ggtattattc gttattcaga
      721 tctttttcaa tggattccat aaacaaatag tcgattgctt cgctcactgt gggcacaatg
      781 tgaagaaccg gatctaattg agaaatggta ataagtttgc gaacggatgg ttgtagacca
      841 actaaaacca atgtgccatt ttcgtttttg cacaatctgt ttcctactaa aacggaagat
      901 agtccagatg aatcgcaata gcgagtatta gtcaaatcaa gaataatatt cactactcca
      961 tctttgttta acatgagtaa atcggacttc aaatcaggtg cgattgtact gtcgagcttt
     1021 tcagctagaa ctctcagaat ggtataatgc tctgtttttt cgtgagcgaa ttgcatactc
     1081 tataacattt gttcaaacct acgtaaaagg attgaatgct acaatgcttc ttacatttca
     1141 ttaaaaagat gaaaaaacga attattcgtt gcgattggca agtaagtaaa gtgctgccat
     1201 acgaacagca actccattct caacttgctg aagaataatc gaatgcgagc tatcagcaac
     1261 atccgaagta atctccactc ccctattaat tggaccggga tgcatgatta caatttcttt
     1321 atttaggttg tcgagtagct ctttattcaa gccataaagc atcgcatact cccttaaaga
     1381 ggggaaatat tggatatcct gacgttcgag ttgaatacgc agcatgtttg caacatcaca
     1441 ccattcaagt gtttttttta gatccgattc tacacgaaca cctaaagatt ggatatcacg
     1501 agggattaaa gtaggaggac cacaaactac tacttcggcg cccatctttt ggaggcaaaa
     1561 aatattcgaa agtgctacac gagaatgaag aatatcacca ataatagcaa ccttctttcc
     1621 ttcaattcct cccaatttcc gacgaatgga aaatgcatct aataatgctt gagtaggatg
     1681 ctcatgtgca ccatctccag cattgattat tttggcattc acatgtttag atagaaagtg
     1741 agcagctcct gctttgggat gacgcataac aaccatatct actttcatag ctaagatgtt
     1801 attcaccgtg tcgataagcg tttcgccctt acttacagat gaagaagagg cactaaaatt
     1861 tataacatca gcagaaagac gtttttctgc taattcaaag gataaacgtg tacgagttga
     1921 gttctcaaaa aatagattga caatcgtgat atcccgaagt gaaggaacct tcttaattgg
     1981 gcggttgatt acatccagaa aattgtcagc agtatgcaaa atgagttcaa tatcttcttt
     2041 ttgaaggtat tttattccaa gcaagtggcg gacgctcaac atagtcagga ttccttgttt
     2101 ttaagtaaga cttctgcatg cgtttcttcc gaattccaat taagtactac ttgttcagag
     2161 aaaatggcat caactgtaag tccggtataa tcgggttgaa ctggtacatg gcgacttaag
     2221 cgacgatcta ccagtacaag taattctaca tttgccggtc ttccgaaatg ttgaagtgca
     2281 tctaaagcgg cacgaatgga gcgaccggta aaaagtacat catcgattag aactactttt
     2341 tttccttcca ccacaaaatc gatagaagta ctagcaggaa ctaatggttt gattcgaaaa
     2401 tcgtcgcgaa aaaaggtcgt atctaattct ccgtaaatca gtgatttccc gagcagttta
     2461 ccgagttcat ttcgaataca tcgggaaaga agaatgcctc gtggttgaag accaatgatg
     2521 acagtgtttt cgaacttatc gtgattttcg atgagttgat aacaaaggcg ttttaaagta
     2581 agctggaagc gttgatgatc aagaatgatc cgatgcgaca tgctgatatt gcttgcgtca
     2641 aatatagcgt attccacctt ttattaatca gttgttattt gattttcgtg ataaggcaaa
     2701 aaaaaacgtc catcggttag gatggacgtt tcgatatagt ttgaaatcaa aaattagtct
     2761 ttcgattttt tagcagcagc ttttttctcg ctttgctcta aatcagactt caaattagct
     2821 aatacgtcaa gatctccaag agttgatttt tcttggctat cttttacttt tttcattgct
     2881 ttcttagtat cgtcagcgcg actttctttc gtttcagatt tctcatcacg agcaccagct
     2941 ttctcttctt cgtgtgtacg agcgtgagaa acgatgattt ttttgctgtc tttagcgaat
     3001 tcaatcactt tgaaatcaag tgtttcttcc gctttagcaa cagaaccatc agctttagca
     3061 aggtgtttat ttggagcgaa accttctaca ccataaggaa gagcaacaac tccaccttta
     3121 tcgttcatag aagtgattgt tccttggtgt actgaacctt ctgtgaagat agtttcgaat
     3181 acatcccatg gattttcttc caattgtttg tgacctaagc ttaagcggcg attttcttta
     3241 tcggtatcca aaacaaccac atcaatgtct tcaccgattt tagtgaattc actagggtgc
     3301 ttgatttttt tgctccagct caaatcagag atgtgaatta agccatcaac accttcttcc
     3361 aattctacaa acacaccgaa gttggtgaag ttgcgaactt tagcagagtg cttagagtta
     3421 actggatatt tagtttcgat atcagcccaa ggatcaggca tcaattgttt aatacccaat
     3481 gacattttac gctcttcgcg atcaaggcta aggatgatac attctacttc atcaccaact
     3541 ttcaagaaat cttgagcaga acgtaaatgt tgtgaccaag acatttcaga aacgtgaact
     3601 agaccttcga agcctggctc gatttcaacg aatgcgccgt aatctgcaag aactacaact
     3661 ttacctttta ctttatcgcc tacgttcaag ttagcatcaa ctttatccca aggatgtgaa
     3721 gtcaattgtt tcaagccaag agcgatacgt tttttacctt cgtcgaagtc aaggataacc
     3781 acctgaattt tctcgtcaag cttaacgatt tcttctgggt gattgatacg accccagcta
     3841 aggtcagtaa tgtggataag tccatcaaca ccaccaaggt caacgaatac accgtaagaa
     3901 gtaatgttct taacagttcc ttcaagtact tgaccttttt caagtttaga gatgatttca
     3961 gctttttgtg cttcaagttc ttcctcgata agtgctttgt gagaaacaac cacgtttttg
     4021 aattctttgt tgattttcac cactttgaat tccatggatt tacccacgta cacatcgtaa
     4081 tcacgaattg gtttcacatc gatttgtgaa cctggaagga aagcttcgat accaaatacg
     4141 tcaacgatca aaccaccttt tgtgcggcat ttcacgtatc ctttgatgat ctcgtcgttt
     4201 tctaaagctg cgttaacacg atcccaagat ttaagtgaac gtgctttttt atgagaaaga
     4261 agaagctgtc cgcttttgtc ttcttttgat tcaatgtaca cttctaaacg atcaccaact
     4321 ttaatttctg gatcgtaacg aagttcagat aatgcaacaa taccatcgga tttgtacccg
     4381 atattaacaa ctgcatcttt tgaagtcata gcaacgataa caccgtcaac aacttcgtgt
     4441 tctgtaattg aacttaatga tttttcatac agcgattcca tgcgctcgcg ctcagatttg
     4501 ctgtagttgc ttgatttttt acctactgaa tcccaatcga aatctaaact agaaatagat
     4561 tcaattggag ttttagaggc tttggacgtt tcagcaactg ctgctggtgc agtagtttct
     4621 ggtgtgtcct gattgatttc tgacattacg tcaataatta aaaaggttaa aagaactgat
     4681 gctcaatgca tcaccctcga ttttcgaagg gctgcaaagg taaaaaatta attagttgaa
     4741 aataagacgg attaaaaaat tagatgaaat tgtataatac ttccatcaac cactgattga
     4801 atgagttatg cgcttttact caattgatgt agccaagtag ttgaagaatc cgagataatt
     4861 tcctccgctt cccaagtgaa aataaaccga gagccttcgc caatattaga ttccaagcga
     4921 attgaaccac cattttcttc gacaatttta cgggcaattg ctaaaccagc accagtactt
     4981 tcaaacttat ctcttgcctc taacgtttga aaaatgacaa atattttttc atgaaaatca
     5041 gcgtgtattc ccggaccgtt gtcgctaaca gtgaatttca attttcccaa ttctgagaat
     5101 aaatctatct gaatggtagg aatttcttgg tgattaaact gaattgcatt tttaattagt
     5161 gcgctgaaaa ctgcttccaa tttcaaagga aaagtcttga ttactggcaa attagcagga
     5221 agttcatagt ttatctcacg accattagaa tgcttcacca ataagccaag tattaaagct
     5281 cgtatgtcga cttttgatat ttcttgattc ttgcggtcag ctcttgaaaa ttctagaaga
     5341 gcagtcaata gatgatccat tcgattcaca cgcccatgaa tgattttgag tttttcatgt
     5401 gtatcgaaac aaagtgattg atcatcttcc aaaatcatat ctgttaatga tccaattgca
     5461 cgaagtggag aacgtaaatc gtgagaagca actgaagcaa atttatcgag ctctgcattt
     5521 ttcttttcta aagcacgatt gtttttctct agtaaagcat tttgtgtttc caaggtgttt
     5581 tttgcatcaa ctaaactctg aataaccaca ttacgaccac tttgcaaata actactcaat
     5641 gccgaaatca tcgcaaatgt gagaagtata ttcaccagaa aatcttcatt tatcaaatca
     5701 atacgatctt gaaaaagaga aaatgaagta aggtcggtgt atgtagctaa aatgaataaa
     5761 tagatgatgt ggattccgca ggcgatgctc attatccatc ctcctctgct acctaaaagc
     5821 atgaaagaat aaataatcac agccgacatg aaaaaagtac caccggttct aattccacca
     5881 caggtatagg aaacaagatg taataaaacg aaacaggata ccatcataat ggtataagat
     5941 cgtttgagat tatctcgctt agaaatactg aagtaattaa gaagaatgat taatccgagt
     6001 aataaaattg taaacggtag ccaattaatc attccaagcg taagcaacat tttagaagcc
     6061 acgctaatac acactaaaat ggctattaca gaaaatatcc gaaagacaac gaaacgctga
     6121 cgttcaatta cttctgttgt aggaatttct tggtaaatat gatctttacg aaacttcttc
     6181 agtaaggatc gagcatagga agtctggctt attttcatag gcgggttggg taaaccttat
     6241 acctaaataa gcttgtaata gtttcgaagg aaaaattagg ctagttcctt atcagtgaaa
     6301 tcatcaatca ataagatcga attgatgaat ttgtcgaatt tgatctgcta tttgctccat
     6361 caaccacatg ggtgttgacg ttgccccaca aataccaacg ctgtcggtag atttaaacca
     6421 attcgactcc acttcttccc attctgaaac aaagtaggta ttggggttta ccgacttaca
     6481 cacatcatat agaactttgc cattcgagct tttcttgccg ctcacaaaaa caattacatc
     6541 gtgtgcttta gcaaactcta ataattgagg ttcgcgatta gacacttgac gacaaagtgt
     6601 gtcgtttgct tcaaagaaga gattttcatc ttgcccttca gcttgcatac gtttgataat
     6661 ctccgcacgc atggctttga atccttcagt aggttgggtt gtttgagaga aaaattgaat
     6721 tggtcgttta aaatctatct gatcaagatc ttcaattgaa ttagcgatta tagctttccc
     6781 attggtttgt ccaacaagtc catttacttc ggcgtggcct tctttgccgt aaaccacaat
     6841 ttgaccatca cctaaatgtt cgtaaccatt acgaattcta ttctgaagtt taagcacaac
     6901 cggacaagag gcatcaatta gttcgatgtt gttctcgatt gcgattttgt aagtttcggg
     6961 tggttcgcca tgggctcgaa tgagcacttt tgtattgtgt aactcacgta gctcttcgtg
     7021 gtcaataatt cgaagccctt tcttctccaa acggtctact tccttattgt tgtggacaat
     7081 atcccctaaa caataaagct cacccgtcgc atttaactca tcttctgcca tttgaatcgc
     7141 gtaaacaacg ccaaaacaaa atccagaatt tgcatcaatg atcacattca tatggcgaaa
     7201 ttaggaaatt atcgggagag agtatttgaa cagtaatttg gtaggaatcg gtatgctata
     7261 aatcaggact taacggtaag ctgatcaact aatttcaaca caaaatcgag ctgttgttct
     7321 cgacttaaat tggaattatc caaaatgatg gcatcgtctg ctttccgtag aggactttcg
     7381 gcgcgactca tgtcttctaa atcgcgttga agtaaattat ctcgtacttg ttcaaaattg
     7441 gcggaaatac cttttgaatc catttcatcc aatcttcgtt gcgttctgat attcgtttca
     7501 gcagtcatga aaattttcag ctccgcatcg ggaaaaacgg tagtaccgat atctcgccca
     7561 tccatagcaa ttcccttccc tttaccgagt tcctgctgga ttttcaccat tttacgtcgt
     7621 acttctccta ctgcactaac ttggctaacc caatcattca catccatttg acgaatgtat
     7681 tgttctacat tttcatcgtt tagcaggatt tcagatgctt gtgtttcggg attaaacaaa
     7741 aattgaatgt gaacttgatc gagctcatca atcaaacctt cttcatccaa ttgttttcca
     7801 ttgattaatc cattccgaag tgcatacaag gtaacagccc gatacatggc tccagtatcc
     7861 acataattgt aatgcaaacg tgcagcaaga gcgcgtgcta tggttgattt cccacaggat
     7921 gaatagccgt cgatggcaat ggtaattttt cgcggaggca ttaatgctta cggtaaaact
     7981 cagataattt tgcggatagc gttatctggt ttggtgagcc ggcaagatgg taagcagaac
     8041 gggcatacgc aagcgaaaac ttgtaaattt ttacaccgag cccccaagat aatccaactg
     8101 tacccaaacg actatctacc ttcatttctt ggcgacgttg ataattgtat cccaaccgga
     8161 atgaaagtcc tttggtaata ttaaactcag ctcctaaaat ggcatggcgg agaattttgt
     8221 tcccaacccc aattttattg atgattgtat cacctgttaa aggatcaatt gtttcggttg
     8281 gcttaagtgg atcatcatag gtcattacag gttgttgaag attatgagca acgaagctaa
     8341 atctcaacgg aacgtgcaat aaacgcttag aaagtcctat ttgtgcttcg aatggaagtg
     8401 tttcacgatt tccatctaca taggaattaa attgataacc gacattttta acaaggacag
     8461 atgcttcgaa aagtttatca ggactaagat atgaagcacc taaatcgagc gccattccaa
     8521 atgaagaata agcttccaag ttggatatta tcgttttcac attcacacca acagaaaaaa
     8581 gagagtctat tttccttcca aaacctgtgg taagacagta ttcgccagca gtaaactcgc
     8641 catttaattg accggttgca tcagctcgtt cgaaattgcc gtaatcgatg tattgcatgc
     8701 tgagtgcgaa agtcccagct tttttaacat taaacgcata ggcggcattt ccgaaattaa
     8761 tcccagcaaa gtattttaca taggtgagag aagtaacgcc agccatggaa gaattgatca
     8821 aaccaggatt ttgataggcc aaatttacat catcatctcg gagagagatt acgcttccac
     8881 ccaaagcctg aacacgagct gagttaggca aattgagaaa actgtaaaca ttttgaccac
     8941 caatttgggc agaaacatcc agctgagcta ggaaaatgag aagaaagaga aatcggaaat
     9001 tcacagtagc taaataacga aataagaatc attcaaaatg acatcaggat aaattcaaga
     9061 atgattgttt tattgtgcga atgtcaattt ttcgaaaatc aacatccata ggaagattga
     9121 aataagtatg tttgtgcttt aaacgaaaag agaatgcaat tcgaccggaa gacagccatt
     9181 gaaaattctg agttacatac ccaagagatt cagattcttg agctatttga taaaaatgac
     9241 aaactgacaa ttttcgatat aggagcatgc gaaggagaga gttcgatacg ctatagcagg
     9301 atttttccga attcgaagat ctatacgttt gaaccgaatc cgtctaattt taagttggta
     9361 gagaaaaata ttgaaaactt ctccagtcga gaacaaatca ctccgtttca actttgtttg
     9421 actgataaaa caggcacagc tgaattttat atttcatcag gaacacctga agaatttaag
     9481 gagaaggaag ttgattggga ttttggaaat aaaagcagtt cgcttcttcc tccagataaa
     9541 acattagaag catttgattg gctgaagttc aacgatgtag ttcaggttaa aactgagcgt
     9601 ctggataatt tgatgaaaga gaacaacatc gatcaaattg atttcattca tatggatgtt
     9661 caaggtgctg aacttatggt tttgaatggt tcaggagaga aaatttctcg catcaataat
     9721 atttggttgg aagttgaagg aattcctttg tacaaaggcc aacctatccg aaaagatgta
     9781 gaaaaatata tgcgtaacat tggttacgtt aagattctcg attcgtttga taaagttgct
     9841 ggcgatcaat tttggtctaa aaaagaatgg ttactgacaa agaaatcgga gaattggatt
     9901 aaagagcgag aacaaaagct taaagaagaa gagaaagctc gtcaaaagcc acttatagaa
     9961 agcattcggg acaaagttca gttgcgatct agaatacgtt ccatactagg agggaaaaaa
    10021 taatttgaag gcatctaggt acatttcaac taaatacaaa gcataaaatt tcgcatgaaa
    10081 gcactagttt ttccgggtca gggagctcaa tttacaggaa tgggaaaaga tttatatgat
    10141 caatttccgg ttgtgagaga cttatttgaa gaagctaatg atatactcgg ttttcgttta
    10201 actgacttaa tgtttagcgg aactgatgag gaattacgtc gtacagatgt aacccaaccg
    10261 gcaattttcc ttcactctgt tgcaattgca cgtttgatgg gagattcatt taaagctgat
    10321 atggttgctg gacattcttt aggagaattt tctgccttag ttgctaataa aacgctttcg
    10381 tttgcggatg gattaaaact ggtttccaaa cgagcatctg ctatgcaagt tgcttgcgag
    10441 caaaacccag gtacaatggc tgccattctt ggattggatg attcaattgt agaggaagtt
    10501 tgtgctggta ttgttggaga aattgttgta ccagcaaatt ataattgtcc tggtcagttg
    10561 gtgatttcgg gtagtttaaa aggtatcgag ttggcttgtg aagcgatgaa agctgcagga
    10621 gccaaacgag ctttaccact acaagttgga ggagcatttc attcaccatt aatggaacca
    10681 gcacgagcag aattagcagc agcaattgca gcaactcaat tctccattcc taattgtccg
    10741 gttgttcaaa atgcaactgc actgcctgct tccgacccag cagttattcg tgaaaattta
    10801 attgcacagc taactgcacc cgttcgttgg acgcaatcgg ttcgaaaaat ggctgatgat
    10861 ggagtaagtc aatttgtaga agccggacct ggaaaagtac ttcaaggact tgtaaagaaa
    10921 attgctccag aagctacagc agcaagtgct gagttagttc aaagctgagt attcaataag
    10981 gaacccgatt cgatcaactt ctttaaagcc ggttcttttc gagcttttcg aaacacatcc
    11041 acatgactca ttttatgtgt atcggttcct aagaaactga ccatattttg ctcaatcaat
    11101 cgctccgaaa tccttttagc tactgttcca taatgaccag ttatagaggc caggtttatt
    11161 tgaagtagta ttcctttatc tcgtaattcc tcgtatttgt taaaactttg ataccaatat
    11221 gggtaacgtt ctgggtgggc taaaactgga atataccctt ccatttgtag tttaaacgta
    11281 actgtttcga agttctctgt taagttcaga tatgaaagtt cgaacagtac atacttctca
    11341 ccaaaggtta gtagcgtttt atcgtcgatg cttttttcaa agttgctatc acagtaatat
    11401 tctgcagcag catgcagttc aactggaatt ccttccagtt tcacagcttc tcttactttc
    11461 tctaaaccac ttaagataat ctcaggagta tttttgtaga aatcactcat gatatgtggc
    11521 gtggtaatga gttttgtata acccaaacta tgcaatccac gaataagatt caagctgtct
    11581 tccatcgaag tagctccatc atcgattccc ggaatgaggt gagaatgaat atcagttctg
    11641 agttggccca ggtctgcttc tgcttgtgca gatccaaaaa tcgaactcag ccaacccata
    11701 tcaaatagat aatacccgga atgcttctaa cgtcttacga agaccttcac gcattttcac
    11761 ttgaggttca taaccgagat acgttttagc aagcgtaata tctgctaatg aatcacgaat
    11821 atcaccttca cgttcttcac gatgcacagg atcgagtcct ttcccagcat attcacgaag
    11881 aatatcatac atctctaaaa cagaaactcg ttcaccaact gctacattat atactttacc
    11941 agtagcttct tcattttcag aaaacaaagc acggatgttt gcttgaacag cattttcaac
    12001 gtaggtaaaa tcacgtgttt gcccaccatc accattcata tacgccggac tagaatccat
    12061 aatcgattga atgaaaagtg gaataacagc agcataaggt ccatcgggtt tttgacgagg
    12121 accaaagatg ttgaagtaac gtaatccagc tattttcaaa ccataggtgc gcccaaaaac
    12181 atccgcgtaa agttcattta cacgtttggt tacagcataa ggtgaaagag gatttccaat
    12241 ttgactttca actttgggta ggactggatg gtcaccataa acactagatg agctagcata
    12301 aaccattcgt tttacaccag cacgacgagc gtgatcaata acggaaagga atccagttac
    12361 gttcgcttca ttagtagcct gaggggtttt aatggaacga ggaacagaac caagtgcagc
    12421 ttgatgggaa acataatcaa ccccattcat agctttttcc atcaaggttt gatcgcaaat
    12481 atccccttcg aaatatttaa acctagcatc ttgaagccaa tgacgaatat tgtcgatacg
    12541 cccttcggag agattgtcga caacgcgcac ttctgcagct ccatgttcga ggaggtaatc
    12601 aactatatgt gatccaataa atccagcacc acctgttacc aagaatactg attgctggaa
    12661 aagagcttcg tttgaatgga agcgaacatg ttgatacatg atcggagtcg ggattaacgc
    12721 ttttcgtact gagaattata atagcgctga tagtcaccag aagtaacagc attaagccat
    12781 tcttcattcg ttaaatacca ctctactgtt cgacggagac cttcttcaaa ttgaagggaa
    12841 ggttcccatc ccagttcgtt tttcagcttt gtaaaatcaa ttgcataacg atgatcatga
    12901 ccagcacgat ctttcacaaa tgtaatcaag gaggcggata aaccgggtgc gcgattcatt
    12961 ttctcatcca ttatctcaca gagtttatga ataagatcaa tgttagtcca ttcgttattt
    13021 ccaccaatgt tatatgtttc acccgaacga cctttatgga agatgaggtc aatggcacga
    13081 gcgtgatctt ctacaaataa ccaatcacga atgttatcac cttttccata aacaggaatt
    13141 ggtcgcatgt gcttgatatt attgatagca agaggaatga gtttctcggg aaaatgaaaa
    13201 gatccgtaat tgtttgagca attcgaaaca actacgggta aatcgaatgt gtgaaagtaa
    13261 gcacgaacca aatggtcgga gctagcttta gaggacgagt aaggagaacg aggatcgtac
    13321 ttagtttctt cagtaaaaaa accagtttct cctagtgaac cataaacttc atcggtagaa
    13381 atatgataga agcgttttcc ttccatattg ccttgccaga tgtgcttggc tgcattaagt
    13441 aggttaacgg ttcctaaaac atttgttcga atgaattctg ttggattgct aatagaacga
    13501 tctacatgtg attcggcagc taaatgaacc acaccatcaa actgttgaag attaaaaagc
    13561 tctgtaatga atgcatcatc aacaatatct ccttttacaa aatgataatt aggtagattc
    13621 tcgatatcct tcagattcat caaattaccc gcataggtca atttatccaa attgtaaatt
    13681 tcataatctg gataattggt aacaaagcgg cgaactacgt gcgaaccaat aaaaccggct
    13741 ccacctgtaa taagaagttt tttactcatt ctgactattt tttgatagtt gtaattccca
    13801 agcccaagcc gactgcatca tttcgaaaag cgatttctca gctttccatc ctagaatctg
    13861 attggattta gaagtatcgg cataaacttt ttcaatgtca ccttcgcgtc tactggtgat
    13921 tatgtattga agtttctgtc cgctagattg ttcaaatgca tgaattgcct ccaaaaccgt
    13981 attcccctgc cctgttccaa tattaaatac ttcgaatgta tcctgttgct gattagacaa
    14041 caaacggtgc atagcagcta catgagcttc tgctacatct tcaacatgaa tataatctcg
    14101 aatacacgta ccatcgggag tattataatc gtttccatat acctgtaatg gtccgagttt
    14161 tccaatggct gattgagtta taaaaggcac taaattattc ggagtacctt taggaaattc
    14221 ccctattagt gccgactcgt gtgcgccaac cggattgaaa taacgaagtg caatgctatg
    14281 cacatccgac actttagata aatcagctaa aatttcttca ccaatttgtt ttgtgtttcc
    14341 gtaaggtgat gttgctggtt ttactggagt agtttcagtt acaggtaaag aatctggttc
    14401 accatacaca gtgcaactcg aagagaaaac gatattgttt actcccgatt cctgcatggt
    14461 ttcaagcaag ttaagcaaag aaccaaggtt gttttgatag tacatcaatg gctttttcac
    14521 cgaatcacct actgctttta gagctgcaaa gtgaattact gcttgaatgg ttggttcgag
    14581 tttgaataat tctctaaccg aagttttgtc acataaatct aattgatgaa aacgaggtct
    14641 ttgagcagaa atctgttcaa ttcgatctaa aatcaagaca tccgaattgc atagattatc
    14701 tgcaataacc acatcatagc cttgtcgaaa taaagcaaca acagtatgag atccaatata
    14761 tcccatcccg ccagttacta gtacacaagg ttttgattga ttcataggtt ttacttataa
    14821 actccagtat tttacatgat tcaattttcc gcgatatact cctttgatat cggctactat
    14881 acctttatcg gtaagcattt cggtaaaata tgattcaggt aatccaacat agtcggagtg
    14941 attcacggct actacaatag catcataaat tccagatgcc tgagaagcga taggaaaacc
    15001 atattcgtga aggaaatcct ttgaactagc atgtgggtcg atgatatcaa catgaactcc
    15061 aaattctaca aattccttca ccacatcagc tactttagaa ttacggatat cagaaacatt
    15121 ctctttaaaa gttgcaccca taattaatac acgcccttta gcgatatcaa ctccttgggc
    15181 caccattttc ttcaccactt tacctgctac atacgctccc attccatcgt taaccgaacg
    15241 tccagcaaga attacctgtg cgttatggcc aacttctttc gccttgaatg tgaggtaata
    15301 tggatcaact ccaatacaat gtccaccaac aagtcctgga aggaaacgta agaaattcca
    15361 ttttgtacca gcagcttcga gcacatcgta tgtattgatg cccatttgac cgaaaatcat
    15421 cgaaagctca ttcatcaatg cgatattcaa atcgcgctgc gtgttctcga taatttttgc
    15481 agcttcggca actttgatag aagcagcacg atgtacgccg gctttcacaa ctatttcgta
    15541 ggtacgtgca atattgtcga gcgattcagc atcacaccca cttactacct taataatacg
    15601 tgatagggtg tgttcacgat cacctggatt gatacgttct ggtgaataac caactttaaa
    15661 atcctcattg aattttaaac cagaaatctg ctccaacaaa ggaatacaat cttcttcggt
    15721 acagcctgga taaacagttg attcatacac tacataatcg cctttcttaa gtgcagcagc
    15781 tacagatcgt gttgcagata agagtggttt taaatcaggt agattatgtt catcgatagg
    15841 tgttggcaca gcaacgataa agaatcgtgc tttacggaga acatccggat cggctgtaaa
    15901 agtgatttca caaccatcga attcttccgg agcaagttct tcacttgggt cgatgcgatt
    15961 gcgcattttc tccacccgtt cggcgttaat gtcaaatcct acaacttgaa tattccttgc
    16021 aaactcaagt gcgattggta gtccaacata ccctaaacct acaacggcta gggaatcttt
    16081 tttctcaata aggttctgat agatagaact cattgtaatt tttttaaagt gcgtagttcg
    16141 taaatgcgtt cgatgatttc gacaacttta agtccttcca aagcattggt agttgcgctg
    16201 gtacgtcctt ttaatgtgtc gattacgttt tgtatcacgt agtgatgatt ggctgcagaa
    16261 cctttgtatg ctccgtagtc gtttgctgga ttagcagcag caagtttggg catttcatag
    16321 tcttgaatgt gacaatattc cacctcattc atgtattgtc cgccaatttt gatacttcct
    16381 ttttctccaa tgatagtgat agaactctct aaattctgat cccaaacgga tgtagaataa
    16441 ttaagtgctc ccattccacc attgtggaaa ttaaatgtga caaatccaga atcttcaaaa
    16501 gccgttgaat tttggtgatt gaagtcgttg aatttggctt gaatgtcttt tatatcacca
    16561 aatagccaaa acatgatatc aataaaatgg ctgaattggg taaacaaggt tcctccgtct
    16621 aaatcgggac tacctttcca acttcctgct ttgtaataac gatcatcgcg attccaatag
    16681 cagtttagtt gaaccatata tacatcgccc aataaacgac gatcgattac atcttttacc
    16741 caaacagaag gcggcgagta gcgattttgc atcacacaaa acacttgtcg cgacatacga
    16801 agggcagtgt atatcacatt ttcacattcg gctttggaaa ggcccatcgg cttttcgcac
    16861 acaatgtgtt tccggttttc gagcgccaat accgaatgct gagcgtgcaa accattagga
    16921 gaacacacat ttaccacatc gaattctaat cctgaattaa ataaggcttc agctgaatcg
    16981 aaatagggta catcgaaatt atctaaacct agtttttcac gaggaagaat atcacaaata
    17041 gcaaccaatt cggcatcttc attacgaaga accatttcgg catgacgctt cccaatatgt
    17101 ccggcaccaa taatggcgaa ttttacttta ttattcaaaa tcatggttta ccgagatttg
    17161 ttacaactcc atgctcaagc ttatactgct cgccactttc gggacaaata gccaatcctt
    17221 gatcattgaa tttcaattta tggccgaatg tactcatcca acccgtttga cgagccggat
    17281 ttccaattac tagcgcataa gctggaactg ttttcgttac cacagctcct gctccaataa
    17341 atgcaaattc gccgatatcg tgtccgcata caattgtagc atttgctcca atagaagcac
    17401 cacggcctac atgagttttc aggtattgat cgcgacgatt aacggcactt cttggattga
    17461 ttacattggt aaaaaccatc gaaggcccaa gaaaaacatc atcgtcacaa ctaactcctg
    17521 tgtaaataga tacgttgttt tgaaccttaa cattattccc tagaacaact tgaggtgaaa
    17581 tcaccacatt ttgtcccaga ttacaatttt cacctaattg gcaattcgac attacatggg
    17641 tgaaatgcca gattttagtt cctttcccaa ttgtgcagcc ctcgtcgata atagctgttg
    17701 gatgtgcgta atagtccatg tgatttatgc cgatgtaaaa ctacgaggtg taataaccca
    17761 ttctggtann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngt ccatgtgatt
    17881 tatgccgatg taaaactacg aggtgtaata acccattctg gtagcgcttt gaaataatcc
    17941 caagtatgct tcatcccttc actgcgtgga actcgaggtt cccaattaag gatttcgcgc
    18001 gcttttgtaa tatcgggctg acgttgttta ggatcgtctt gaggaagtgg tttgtataca
    18061 attttctgat cggtaccagt aagtttgatg atttcctccg caaagtcgcg aatgctgatt
    18121 tcatctgggt taccaacatt aactggataa gcataatcgc tcaataatag ccggtaaatt
    18181 ccttctatta aatcgtcgac atagcagaac gagcgagttt ggctaccatc gccaaataca
    18241 gtgatgtctt caccacgcaa tgcttgtccc ataaaggcag gaagtgcacg tccatcattc
    18301 agacgcatac gtggcccata ggtattgaaa atacgaacga ttcgagtttc tacgccatgg
    18361 aatgtatgat acgccatggt gatcgcttct tgaaagcgct ttgcttcgtc gtatacacca
    18421 cgaggaccaa ttgggtttac atttccccaa taatcttcgg tttgagggtg aatggtagga
    18481 tcgccatata cttctgaagt agacgcgata agcattcgag cacctttcac gcgagccaat
    18541 cctaatagat tatgtgtacc taacgagcca acttttaagg tttgaatagg gatttttaaa
    18601 taatcgattg gacttgctgg tgaagcaaaa tgtaagatgt aatcgatatt gccaggaaca
    18661 aaaacgaact tggaaatatc gtgatggtaa aactcgaagc gttcttcttt gaagagatgt
    18721 tcaatgtttt gaagacttcc tgtgataaga ttatccatcc caaccacata gcaaccttcg
    18781 gccatgaaac ggtcacacaa gtgagagcca agaaaaccag cagcgccggt aattagaacc
    18841 cgttttcctt tgatatcgta tttcattttg ctttacgaac agatttacga ccaatgctga
    18901 aatagcttag tccaagtttt ttcatttgat caatggtata cagatttcta ccatcgaaaa
    18961 tgactttgta cttaatttga gaaatcatgc gctcaaaatc tggagtacgg aaagccgacc
    19021 attcggtaca gataacaagt gcatcggcac cttcaatggc ttcgtattga ccttttgcga
    19081 attcgatttt acgacctgtc tttttcacat tagccattgc ttcaggatcg taagcacaaa
    19141 cctcagcacc agcagctaat agttcatcaa tgatatacaa agcaggagct tcgcggatat
    19201 catctgtatc gggtttgaaa gctaatcccc aaatggcgat acgtttacct ttcaaatcgt
    19261 tattaaagaa cttattgatt ttaggcatca atttcacctt ttgtttttca ttcacttcca
    19321 tcaccgaatg gagaatactg aagtgataat ccacatcgct tgatgactta actaatgcct
    19381 gaacatcttt aggaaagcaa cttccaccgt aacctatacc tggaaaaagg aaacgtttac
    19441 cgatacgatc atccgaacca attcccatac gtacttgatc cacatcagcg cccaacaatt
    19501 cacacagatt ggcaatctca ttcatgaacg agattttggt tgctagaaaa gaattggcgg
    19561 catacttagt caattcggca gaacgctcat ccataaatag gataggatta ccctgacgaa
    19621 cgaacggcaa gtataattct tccatgactt tgcgggcacg agaagaagaa gtaccaatca
    19681 ccacgcgatc gggtttcatg aaatcttcta ctgcaaaacc ttcgcgaaga aattccgggt
    19741 ttgaaaccac atcaaattct gcctttgcat tctttgctaa agctttgtga actttttcgg
    19801 cggttccaac aggaacggta cttttatcaa tgatgatttt ataggaatca gtcaggagat
    19861 gaccgatatc ttctgaaaca cctaaaatat aggaaagatc ggcagagcca tcttcacctg
    19921 gaggtgtagg taaagcgaga aaaatcaatt cagaagcatc gattgcttct tttaaatcgg
    19981 tagtaaaatg taaacgacct tgctttaggt tacgttcaaa aactacatct aattgaggtt
    20041 cataaatcgt aacagttcct ttgcgaagtt tggcaatttt atctttgtca atatccacac
    20101 aaataacatt gttacctgtt tcggcgagac atgttccagt aactagtcca acgtaaccag
    20161 tacctattac agcaatattt ttcatgttag tttgacttag taaattccaa aacagtagaa
    20221 ataatcaaat
//
LOCUS       DAOOQW010000026        19979 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_3300_length_19979_cov_5.447149, whole genome shotgun sequence.
ACCESSION   DAOOQW010000026 DAOOQW010000000
VERSION     DAOOQW010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 19979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19979
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3300_length_19979_cov_5.447149"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2233
                     /locus_tag="PK281_03080"
     CDS             <1..2233
                     /locus_tag="PK281_03080"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOY48355.1"
                     /translation="DKGICQYRSRENKLVIPTDFTHWNSGQINSISILGSDLWIATEK
                     AGCINYNPSLFDGIRSFNSSENINFSKCNDLVFDQEGNCWIATQAGLIRSSANSIRSI
                     DEINASQLSKIQSILCSQSGDVFFANNQFLSRISVRGNNLDTRKILITNSTAKTDMSC
                     LFEDKHGFIWIGTMGNGLFCFDPVSGQKVKLPGFSDLNSSSILSLAIQGNKIWITTIG
                     GVLLGDIFYPTSNISGIHIKDLHLVTEIGNNIVYKVIIDSKNRCWFASDGNGITCYDG
                     NKYITFNSKNKLKSDIIFGITEDTNKNIWFCTADAGVYSFDGSNFINYGLKEGLHELS
                     SSAIIADKSGVVYCIHQQGVDAIDIRSKQVTYFGNEYLFNDINPDLNSVSIDQSGTVW
                     IGTSKGLILLNSKYRTMNKQPVSLIKRVSLLGNDSANTNLKVFNYDQNSLSFHFAGIW
                     YSDPGRVSYRYLLKGYSKDWIETKDNQVVFPELTPGEYEFILQTGLGKSFNHTTQTSY
                     SFRIKSPFWDEIWFRIPVLLLILAAIYGYFKWRDGEIRRYQTLENEKVRFQLNTLKNQ
                     VNPHFLFNSFNTLISIIETDSATAVKYVENLSEFFRNVVTSQNKDLITLKEELEHSAS
                     YYFLQKKRFGNNLLIDFEIEPEKEQLFLPPLVLQLLIENAIKHNVCSKDSPLHIRIES
                     QGSHLIVCNNLNEKRTVEASTKTGLKNIIDRYKLLSSENIDILKSNTEFRVTLPLLTS
                     DKA"
     gene            2230..2988
                     /locus_tag="PK281_03085"
     CDS             2230..2988
                     /locus_tag="PK281_03085"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOY48356.1"
                     /translation="MKVLILEDEEPASQRIAKMLLELVPDAQVLDILDSVKSSLNWFG
                     TNEMPELILADIQLADGISFELFNTIEISCPVIFTTAFDEYAINAFKHNGIDYLLKPI
                     KKEELQISLNKFMKLKESSLKPDYSELLHVLGKQNNNFQKRIIIRFRDQFIMVDVDEV
                     ACFFVENKITYLVTRQNQQYQVDYTMDECENLFNPKLFFRINRQVIVHIHAISKMVAY
                     SKSRVSLSLQPNVPFETIVSTERSAAFKLWLLGK"
     gene            3141..5180
                     /locus_tag="PK281_03090"
     CDS             3141..5180
                     /locus_tag="PK281_03090"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOY48357.1"
                     /translation="MNWTNYFRFLFLLLVFACTDNAHAQIQLAFQGGESGDNWTYTAT
                     AVSSVGTSEALLIPNIVSGSHSLVVGGVSGGGSCITSGSGNGLSIFHNFTFADIDIST
                     SASVSRQLKFNYGNRMPACTGTGWDSGEDMVFTPIFDGIPQAAITIFTGATNATVDIH
                     QTQYTFSIPACVGTFGFIVGVTTNRDDEFLFIDNVELTTPQFNGPIPSFSTIDGPIQA
                     CTGTSSIFSTDIYSGVNYHWFNNPSTSTFISPNDSTVADSITLDWGTTLPGTYTISVS
                     PTDACGLLNGDTISITVELIDNPQPLTIIGPDTICQGEIATFSSNYLTGNVWSTLDTT
                     QSIQVSQSGTYSVSAIGACGSMQSNVTLTVIPPSEATILINGSLALCPGQMVQLISST
                     DQNFSWSDQSLNDTLTVTEAGLYILTVSNACGTTADTVQVVAVSGNLQPEILSSGSVI
                     CSGSSLLLTSSENNGNSWSTGSLNDTILVTTPGTYILTVDNGCEIASDTLQVTSSTLS
                     FTTEALPDSGNAPLDVNFLVNSPNVISSYSWDFAGLGSSSSATPNFIFNEAGLYSVIV
                     EVTNDAGCSGLDTIEVLVLPELPSDLNVPNCFSPNGDSINDYFTVDATSIKTVDIQIL
                     NRWGIKMYQSSELTPGWDGKFNSENAPDGTYFYVLKAEGNDSKSYEFKGTLTLIR"
     gene            complement(5188..5490)
                     /locus_tag="PK281_03095"
     CDS             complement(5188..5490)
                     /locus_tag="PK281_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013684944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha
                     C-terminal domain-containing protein"
                     /protein_id="HOY48358.1"
                     /translation="MSAKIKILKTCKNGHSFYKSSDCPVCLICENNRKPNADFLLKLS
                     APARRALENNGITTLELLAQYSEKELLQLHGFGKSSIPKIASFLSEKGLQLKSIFD"
     gene            complement(5494..5904)
                     /locus_tag="PK281_03100"
     CDS             complement(5494..5904)
                     /locus_tag="PK281_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48359.1"
                     /translation="MNSTVRNILAVLAGLFVGAVVNGTIIMISGKIIPPPEGAILTTP
                     EGLIASMHLMQPKHFIMPWLAHALGTLVGAIVTVLIAASYKQRLSYTIGFIFLAGGIA
                     NVFMLPSPLWYTLVDLLGAYLPMSYLASKIALKK"
     gene            complement(5968..6522)
                     /locus_tag="PK281_03105"
     CDS             complement(5968..6522)
                     /locus_tag="PK281_03105"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOY48360.1"
                     /translation="MKGYTGTYNLKNGLVITIRHVRIEDATKLIETKRKYIAESPYIP
                     INIEEYTQTIEQEEQLISNYINQSNSLLLVAEFEGEIVGNIDLTGSTRKQLAHTAMLG
                     IGMLPAFQGLGLGTLLLKHALDWAQSNEILEIIWLEVIPENSFAVKLYTQSGFVVAGS
                     IPNFFKQEKHQLDKLTMYMNVNSN"
     gene            complement(6560..7012)
                     /locus_tag="PK281_03110"
     CDS             complement(6560..7012)
                     /locus_tag="PK281_03110"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HOY48361.1"
                     /translation="MNTITIRPYQNSDHASLLEVIQLNTPKYFAQEEKNELKHYLEKD
                     VEDYYIVETSDRIVAGGGINYPINQDFATISWDLIHPDFQGKRIGSDLLRFRISHIQK
                     KGFSKIRVRTSQQAFGFYEKHNFVLNEIIPNYWAKGYDLYDMYLVIVD"
     gene            complement(7139..9340)
                     /locus_tag="PK281_03115"
     CDS             complement(7139..9340)
                     /locus_tag="PK281_03115"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY48362.1"
                     /translation="MKTLLPFALALLSVLEASSQNLSLSWVKQFGGPSNMTPLGMAKD
                     PSGAIITSGFIEGVTDFDPGPESNPASTNGLKDAYISKLNSNGDYVWSVAFGGSGNDL
                     AYDVATDVAGNVYCTGEFTGTVNFNPQGTAANLTAFFGYNAFIVKYSASGQFQWVKKF
                     GGASSGYESGRNISIDGSGNIITTGQFYDLVDFDPNSGVATTGLVSSYSCYMSKLTSN
                     GDYVWAKSIPGYYTTCSELNIDANNNIYWVGYFYGSTDFDPSAANYSLVGQYYDTYIA
                     KYTPQGNFTWVKQLTSTNYCFTYALEFATDGSLFLAGGFNGSTDFDPDLVATQTVTNT
                     SGSYHPFLLKLSNSGLFQFVKELAPSSVGSATGVKLDSDGNIYVGGHFKGTSDFNPDP
                     VDEFPMTSSSTNYEDAFITKLNSAGDFISAYSFGGTSSDYVNGLQINPANGTLSVIGN
                     FSGTCDFDPSSDVMNLTSAGGSDGYIARFVQCSAVSATIQINACGSYDLNGTNYTESG
                     TYSQTLESSLGCDSTLTLELTISGATFSNITLYTNDVITFNGQSYGEAGQYTQTISNQ
                     AGCDSIIYVEVVILENNFELEIDNGIITTPQLGNSYQWIDCNNNNLPITGETNSTFTP
                     TQSGSYALIVYGNEGDVTSNCIDVIVTGNQDIYESAIQFFPNPLIDQLIIKSEKEVFN
                     SYQIYDMQGKLVLAGNITQNIESISIRDLSSGIYSIQLIGQHMFTSKIVKQ"
     gene            9480..11465
                     /locus_tag="PK281_03120"
     CDS             9480..11465
                     /locus_tag="PK281_03120"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOY48363.1"
                     /translation="MRFRFSFFIIGLLSSLGVNGQSEEELLRQLQATKNDSLRFFIQR
                     DLGYLFEFTDSAKAMGYYSMNLPLAQELNSKRLLSMVWLDFGRVQSNFKHTDSARKYY
                     LKSLQVAEASPDYKRVGVALTNIGNTYLAEYRFDLAASYLFKAISAQEKALDSLNLAL
                     SYSTLGVLFEDAGKFDNALFYLKKCKEISLLNHNYQTYLSASITEAVIYLKKGDMQSM
                     HFTINKALVILSKYNHLYYKASFYQNISGLFFDSNDFLKANQYADSSLYYHTLNDPES
                     IDAATYMSKGLALIKLNQIDLGKTYLVKALNLAIEVEDWQVAREIYLELAEIAAARGD
                     WKSAYENHTKYVECKDSTYTEELAKNASELSIKFQSEKQQLKIDKLAEENMINTLLAK
                     QERRKGWFMLAGGSLLALIFILLFIIQRRKNQFIQKETELQLEQIKRLESEKKVVVLD
                     SMLKGEENERSRVAKDLHDGVGSLLSGVKLSLSSMQGNMIIQEEHARVFEKSLLQLDD
                     AISEMRRVAYNMMPAVLHHSGLIDATRSLAHSLSEASGISIHFEHHFLDERLPNEYEI
                     ILYRLIQELLNNALKHSQASNIIVQLNAHENHLSLVVEDNGKGFDLSLWEKSTGMGFH
                     NLKNRVEYLNGIIHVTSSSEEGTSFLIEFELNHAKEA"
     gene            11449..12075
                     /locus_tag="PK281_03125"
     CDS             11449..12075
                     /locus_tag="PK281_03125"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOY48364.1"
                     /translation="MLRKLNILVVDDHPVVLEGMLNILKQLDFIAEIHKAEEAISAMS
                     IVRRFAIDVVITDINLPEINGIELGRKIHAEFPSINIIGMSTFHDPLYVGELIQAGGK
                     GFITKNSTVSEIESTIRGVMEGQVIISHLLNNQKQVSFPDSQGPIITRREKEVLALIA
                     EGCTNKEIAEKLFVSLSTVDSHRKNLLAKFEVMNTASLISKAMKSGVL"
     gene            complement(12194..13270)
                     /locus_tag="PK281_03130"
     CDS             complement(12194..13270)
                     /locus_tag="PK281_03130"
                     /inference="COORDINATES: protein motif:HMM:NF017984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HOY48365.1"
                     /translation="MSFTLTNIAHLRLAAQQLLNPSYTTAHDLVSWLGAVQAQEYAQT
                     IWGLGLRLAFCLASDIEREFNEGRILRTHLLRPTWHFVTAEDIQWMLALTAPRVHAVN
                     KFMYRQTELNESIFRRCNELIIQSLQGVKQLTRDELNEVFKQHSIEASGHRLSYIMMH
                     SELNGTICSGAKKGNQHTYALISERITQVPQLTIDEALIELTKRFYQSRGPATVNDYS
                     VWSGLTLTECRKGINSLGKELISYQLEGALYYFTQQQTEISDKPTRMLLLPMYDEYVM
                     GYKDRSAIFVNRNNLKPKPKSLHNCIIIWNGQVIGTWKRTVTSKTMNVEYHFFEKLSI
                     EQNLAFEQALEDLENFSKLKLSLK"
     gene            complement(13318..14460)
                     /locus_tag="PK281_03135"
     CDS             complement(13318..14460)
                     /locus_tag="PK281_03135"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY48366.1"
                     /translation="MSLKSIFLFYLILPLNQLLAQATPLFDDEKKVEIAGLTFDAMEP
                     FISNDGSTLFFNSLNSGGNTNLYYALRINDSTFTYMGLVGGCYDSSPNHLDGVASLDM
                     LNNFYWVSLRNYPIQIENLHKGNYTSGNVSDITRVYGNFNSYIPGWLIMDAAITHQGN
                     ELYYCNAFFNNCSNGTPCETHMGVALYVNDSTFNKAPNSDFIFQNINDTNYINYAPQV
                     SNDGLELYYTRLQLNSINTEICVSVRSSLSESFGTPTVIHSNIGFVPEAACLTSDNQI
                     IYYHQKDASGIFHIYSRRKTSSLGLNQIEKSQIKFFPNPANDFININLSLSSEPFMIE
                     IYSSFGQKVISSTNIKRIDISTLNNGLYHLKVIQNRKLDFYTFIKN"
     gene            14781..18149
                     /locus_tag="PK281_03140"
     CDS             14781..18149
                     /locus_tag="PK281_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HOY48367.1"
                     /translation="METSVYQPQHKIRIVTAASLFDGHDAAINIMRRIIQSSGAEVIH
                     LGHDRSVEEVVNCAIQEDAQAIAMTSYQGGHVEYFKYMYDLLHEKGAGHIRILGGGGG
                     TILPSEIEELQQYGISRLYHPDDGRSMGLQGMINDLMSRCDFDTVTQLNGEVNHLAEK
                     DWKSIARLISMAENHPEAVGNSFDRIHELAAESKSPVLGITGTGGAGKSSLVDELVRR
                     FLIDFPEKHIGVISVDPSKRKTGGALLGDRIRMNAIRNPRVYMRSLATRQSNLALSKH
                     VKEAVAVLKAANYDLIILETSGIGQSDTEILDHSDVSLYVMTPEYGAATQLEKIDMLD
                     FADLIALNKFDKRGALDALRDVKKQYQRNHNLWDTPPEEMPVFGTIASQFNDPGMNNL
                     YRRIMDTLVERTGADLKSGYTVTQEMSEKVYVIPPNRTRYLSEITENNRNYNSWVEKQ
                     SEVAEKLYAINKTMDTMKSTVNADILKVLQAEFDRVKLELDPKNWLLIEQWEAKRQSY
                     KDEFFIFKVRDKELKIATHSVSLSQSRIPKVVTPAYKNWGGILRWSLTENVPGEFPFA
                     AGIYPFKREGEDPTRMFAGEGGPERTNRRFHYVSKGMPAARLSTAFDSVTLYGNDPDL
                     RPDIYGKIGNSGVSICCLDDAKKLYSGFDLCAPSTSVSMTINGPAPMLLGFFMNAAID
                     QQCEVYIRQNGLLDEVNAKIDALFAAKGMKRPMYQGELPEGNNGLGLLLLGVTGDQVL
                     PADVYAGIKAKTLSVVRGTVQADILKEDQAQNTCIFSTEFALRMMGDIQQYFISEKIR
                     NFYSVSISGYHIAEAGANPITQLAFTLSNGFTFVEYYLSRGMHIDDFAPNLSFFFSNG
                     IDPEYAVIGRVARRIWAKAMKLKYGGNERSQMLKYHIQTSGRSLHAQEIDFNDIRTTL
                     QALYAIYDNCNSLHTNAYDEAITTPTEESVRRAMAIQLIINKELGLAKNENPIQGSFI
                     IEELTDLVEEAIYTEFDRITERGGVLGAMETMYQRSKIQEESMYYEMQKHTGEFPIIG
                     VNTFLSSKGSPTVLPKEVIRASEEEKQYQIDMLKDLHTRNASRIDELLRKLQEAAVLN
                     QNIFEQLMEVSKYASLGQITSSLFEVGGQYRRNM"
     gene            complement(18459..>19979)
                     /locus_tag="PK281_03145"
     CDS             complement(18459..>19979)
                     /locus_tag="PK281_03145"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrobacter succinogenes major paralogous
                     domain-containing protein"
                     /protein_id="HOY48368.1"
                     /translation="NAIQLNGQPYTGTVNVYAQALDPTSPEMFDQMPGELLGGMNDSL
                     RLLRSFGMASIELRDTSMNELQMANGVSASLTFNIPTALQAEAPETIDWWSFDESLGY
                     WKHEGEAIKQDSQYVGQANHFTWWNYDVPQNFNDFNGTINSVSGTPVSDAQVNVVTQT
                     LGTGVTYTNSEGVFTGRVPKNQQLTLNVYLTCSTTNDWNLAHSETIASETQPLSIVLT
                     ANLENRYPITGTVVNCEGQPVDLGYVKMNGRIFILDNGTFSIQTCQMGTYSIRSYDIS
                     NSDSVNVSNLIDVQVGISGVDAGMIATCSTMFGSVSDIDGNLYSTVLIGDQWWMAENL
                     KTIHFADGTLIPNITESLTWSQLSSPAWCNYNNSILYGSIYGKLYNWFTVADPRNVCP
                     DGWHVPSDTEWTLLTDYLGGFSTAGGKMKAITGWNAPNTGANNESLFTGLPGGYIQVI
                     GNFSTFLHIVYSGMWWSNSEFSLSEAWYRNLVFNYDVAAYNNTNKKNGFSVRCIKD"
BASE COUNT         6215 a         3577 c         3740 g         6447 t
ORIGIN      
        1 ggataaaggg atttgtcagt atcgttctcg agagaataaa ttagttattc cgactgattt
       61 tacacattgg aattcggggc agattaatag tatatcaatt cttggaagtg atttgtggat
      121 tgcaaccgag aaagcaggtt gtattaatta taacccatca ttatttgatg gaattcgttc
      181 atttaactct tctgaaaata ttaacttttc taaatgtaat gatttagtat tcgatcaaga
      241 agggaattgt tggattgcta cacaagctgg tttaattagg tcgagcgcaa atagtataag
      301 aagtattgat gaaattaatg caagtcaatt aagtaaaatt caaagtatac tttgttctca
      361 aagtggtgat gttttttttg caaacaatca atttctttcg cgtatttcag tcagaggaaa
      421 taaccttgat acgagaaaga ttttaattac taattccact gccaaaaccg atatgtcgtg
      481 tttatttgaa gataaacatg gattcatttg gattggaaca atgggtaatg gtttattttg
      541 ttttgatccg gtttcaggtc aaaaagttaa acttccaggg ttttcggatt tgaactcatc
      601 cagtattttg tcacttgcta ttcagggtaa taaaatctgg ataacaacaa ttggtggtgt
      661 tttactcgga gatatttttt atccaacttc taatatttca ggaattcata ttaaagatct
      721 tcatctagta accgaaattg ggaacaatat tgtttacaaa gtaattattg attctaaaaa
      781 tcgttgttgg ttcgcttctg atggaaatgg gataacatgt tatgatggaa ataaatacat
      841 cacattcaat tctaagaaca aattaaaaag cgatatcatt ttcggtatta cggaagacac
      901 gaataaaaat atatggtttt gtactgccga tgctggtgtt tattctttcg acggtagcaa
      961 ctttattaat tatggtctaa aagaaggatt acatgaattg agctcttcgg caatcattgc
     1021 agataaatca ggagttgtat attgtattca tcagcaaggt gttgatgcga ttgatattcg
     1081 atccaagcag gttacttatt ttggtaatga atatttgttt aatgacataa atccggattt
     1141 aaattctgtc tcaatagatc aatcaggaac agtttggata ggtacctcta agggattgat
     1201 tcttttaaat tccaaatata gaactatgaa taaacagcct gtttcactta tcaaacgggt
     1261 tagtttatta ggaaatgata gtgctaacac aaatttgaaa gtgtttaatt atgatcaaaa
     1321 tagtttatcg tttcattttg cgggcatttg gtattctgac ccagggagag tgagttatag
     1381 atatttgctt aaaggataca gcaaagactg gattgaaact aaggataatc aagttgtctt
     1441 tcctgaactt actcctggtg aatatgaatt catactgcaa actggattgg gtaaatcgtt
     1501 taaccatact actcaaacaa gctattcatt tagaataaaa tctccttttt gggatgaaat
     1561 atggtttaga atacctgtat tgttgttaat acttgctgcc atatatggtt attttaaatg
     1621 gcgagatgga gaaatacgtc ggtatcaaac tctcgaaaat gaaaaagtga gatttcagtt
     1681 gaatacattg aagaatcaag ttaatccaca ctttttattt aatagcttca atacccttat
     1741 ttccataatt gaaactgatt ctgccactgc tgttaagtat gtagagaatt tatcggaatt
     1801 cttccgaaac gtagttacat cacagaataa agatttaatt actctcaaag aagaattaga
     1861 acattcagcg tcctattatt ttcttcaaaa aaagagattc gggaataatt tactcataga
     1921 ctttgaaatt gaacctgaga aggaacagct ttttcttcct cctcttgtat tgcaattgct
     1981 cattgaaaat gctatcaaac ataatgtttg ctcaaaagat tcacctttac acattcgtat
     2041 cgaatcgcaa ggaagtcatt tgattgtgtg caataatctt aacgaaaagc gcactgtaga
     2101 agcttctaca aaaacaggtt tgaagaatat cattgatcgt tataaattgc ttagttctga
     2161 gaatattgat attcttaaat ctaatactga gtttcgagtg acacttccat tattaacatc
     2221 cgacaaagca tgaaagttct aattttagaa gatgaagaac ctgcatcaca gcgaatagca
     2281 aaaatgctat tggaattggt gcctgatgcc caagttttag atatacttga tagtgttaaa
     2341 tcaagcctaa attggtttgg tactaacgaa atgcccgaat taattttagc tgatattcaa
     2401 ttagctgatg ggattagttt tgaattattt aataccatcg aaatatcttg ccctgttatt
     2461 tttaccactg ctttcgacga gtatgctatc aatgctttta aacataatgg gatagattat
     2521 ttattaaaac ctattaagaa agaggaatta cagatatcac ttaataaatt tatgaaactc
     2581 aaagaatctt ctctaaagcc cgattattca gagcttttac atgtattagg taagcaaaat
     2641 aataacttcc agaaacgaat cattattcgt tttcgtgatc aatttattat ggttgatgtg
     2701 gatgaagttg catgtttctt tgtggaaaat aaaataacct atttagttac acggcaaaat
     2761 caacaatacc aagttgatta taccatggat gaatgtgaga atttgtttaa tcctaagttg
     2821 ttttttcgaa ttaaccgaca agtaattgtg catattcatg ccatttcaaa aatggttgct
     2881 tattccaaat cgcgtgtttc actaagcctt caacctaatg ttccttttga aacgatcgta
     2941 agtactgaac gtagtgcagc attcaaacta tggttattag gtaaataact gagagctttt
     3001 tggatttgat ggctcatttt ctacttggtt tggcaattag actactcata taaagtactt
     3061 tattgatatt tactgatagt ttgctttatc agtaacattt ctataacatg ctactcgttt
     3121 gcatttagta gtttcgcagc atgaattgga caaactattt tcgctttctg tttctcttac
     3181 tagtatttgc ttgcactgat aatgcacatg ctcagataca attagctttt caaggtgggg
     3241 aatcgggtga taactggact tatactgcaa ctgccgtttc ctctgttgga acttctgaag
     3301 cattactaat tcctaatatt gttagtggtt cgcattcact tgttgtagga ggagtttcag
     3361 gtggaggaag ttgtattaca agtggctcag gaaatggtct tagcatcttt cataatttca
     3421 cttttgctga tatcgacatt agtacttctg cttcagttag tcgtcaactc aaattcaatt
     3481 atggtaatcg aatgcctgca tgtacaggta ccggttggga ttcgggagaa gatatggttt
     3541 tcactcccat ttttgatggc attcctcaag ctgctataac tattttcact ggtgctacta
     3601 acgcaacggt agatattcat caaactcaat atacgttctc aatccctgct tgtgttggta
     3661 cctttggatt tatcgtagga gtaaccacta atcgtgatga tgagttttta ttcatcgaca
     3721 atgtcgaatt aactactcct caattcaatg gcccaattcc ttctttctct actatagatg
     3781 gaccaataca agcatgtaca ggaacttcat ccatatttag tactgacatc tattcagggg
     3841 ttaattacca ttggtttaat aatccatcaa catcaacttt tatttctcca aacgattcaa
     3901 ccgtggccga ttcaattacg ttggattggg ggacaacttt accgggaact tatacgattt
     3961 ctgtttcacc aactgatgca tgtggactat taaacggcga taccatttca ataacagttg
     4021 aactaattga taatcctcaa cctttaacta tcattggtcc agatacaatt tgtcaaggtg
     4081 aaatagccac atttagttcg aattatttaa ctggaaatgt ttggtcaact ctagatacta
     4141 ctcaaagtat ccaagttagt caatctggaa cgtattctgt tagcgcgatt ggtgcatgtg
     4201 gtagtatgca aagcaatgtt actctaacgg tgattcctcc ttctgaagcg actattttga
     4261 ttaatggcag tttagcttta tgtccaggtc aaatggtgca acttattagc tcaactgatc
     4321 aaaatttttc ttggtctgat caatcgttaa atgatacttt aaccgttaca gaagcagggc
     4381 tatatattct gactgtttct aatgcatgtg gtacaaccgc cgatacagtt caagtggttg
     4441 ctgtttcagg taatttacag ccagaaatct tatcttcagg ttcggtaatt tgctctggtt
     4501 caagtttact acttacttcg agtgagaaca atggaaattc atggtcaacg ggaagtttaa
     4561 atgataccat acttgttacc actcctggaa cttatattct aacagtcgat aatggttgcg
     4621 agattgccag cgatacgcta caagttacaa gttcaaccct aagctttact accgaagcat
     4681 taccggattc tggaaatgca cctttggacg tgaatttttt agtgaattca ccaaatgtga
     4741 tttctagtta tagctgggat tttgctggat tgggttcaag ctcatctgca acaccaaatt
     4801 ttatttttaa tgaagcaggt ttgtattctg ttatcgtcga agttactaat gatgcaggct
     4861 gttctggatt agatactatt gaggttcttg ttttgccaga attaccttca gatttaaatg
     4921 ttccgaactg ttttagtccg aatggagata gtattaacga ttattttacg gttgatgcaa
     4981 caagtattaa aactgttgat attcaaatat taaatcgttg gggaattaaa atgtatcaat
     5041 cttctgaatt aactccaggt tgggatggta agttcaactc agaaaatgct ccagatggaa
     5101 catattttta tgttttgaaa gccgaaggga atgattctaa atcctatgaa tttaaaggaa
     5161 ctttaacttt aattcggtaa tatttcttta atcaaatatg gattttaatt ggagcccttt
     5221 ttcggaaaga aaacttgcta tttttggtat agatgatttt ccaaaaccat gtaattgaag
     5281 caattccttt tccgaatatt gggctagcaa ttcaagtgta gttattccat tattttctaa
     5341 tgcacgtcga gctggcgcac ttaattttaa tagaaaatcc gcattgggtt ttcgattatt
     5401 ttcgcaaatg agacaaacag gacaatcgct tgatttgtaa aagctatgcc catttttaca
     5461 agtctttagt attttaattt tagctgacat ttttcacttt ttcaaggcga ttttactagc
     5521 taaataactc attggtaaat aggcacctaa taaatcaact aaagtatacc ataggggaga
     5581 agggagcata aatacatttg ctattccccc agcaagaaaa ataaaaccaa tggtatatga
     5641 caaacgttgt ttgtaggagg ctgcaattaa tacggttaca attgcgccaa ccaaagtacc
     5701 taatgcatgt gccaaccaag gcataataaa gtgcttaggc tgcattaaat gcatagaagc
     5761 tattagtcct tctggagttg ttagaatagc accttcagga ggaggaatga ttttcccact
     5821 aatcataata atggttccat taacaacggc gccaacaaaa agtcctgcta gtacagctaa
     5881 aatgtttcga actgttgaat tcatcattta caaatttggg ttggggttta attccaaaga
     5941 ataattactt ggattagcta atataaacta attcgaattc acattcatat acatggttag
     6001 cttatctagt tggtgttttt cttgtttgaa gaaattggga atacttccag caacaacaaa
     6061 acctgattgg gtgtatagct taaccgcaaa gctattttcg ggtattactt ccaaccagat
     6121 aatttctaga atttcatttg attgagccca atctagcgca tgttttaaga gaagagtccc
     6181 aagtcccaaa ccttgaaaag cgggcaacat tcctatgccc aacatagccg tatgtgccag
     6241 ttgctttcga gtggaccctg ttaaatcgat atttccaaca atctctcctt cgaactcagc
     6301 tactaataat aatgaattag attgatttat ataattagag atgagttgtt cttcttgttc
     6361 gattgtttgc gtgtattcct caatatttat tggtatgtaa ggactttcag caatgtattt
     6421 tcgcttggtt tcaattaatt tagtggcatc ttcaattcgt acatgtcgta tagttattac
     6481 taaaccattt tttagattgt aggtgcctgt gtatcctttc atacttgtgt tgaaatataa
     6541 cgcgttagcc ttctaattat caatcaacta tgactagata catgtcataa agatcgtatc
     6601 cttttgccca ataattcgga attatttcat ttaaaacgaa attgtgtttt tcataaaatc
     6661 caaatgcttg ttgtgatgtg cgtacacgga ttttgctaaa tccctttttc tgtatgtgac
     6721 ttattcgaaa cctgagcaaa tcacttccaa ttctttttcc ttggaaatcg ggatgaatta
     6781 agtcccagct aattgtcgca aaatcttgat ttattggata atttattcct cctccagcaa
     6841 caattctatc actagtttcc acgatataat aatcttctac atccttttct aagtagtgtt
     6901 ttaattcgtt cttttcttct tgagcaaagt atttgggtgt atttagttga attacctcca
     6961 atagactcgc atgatcagaa ttttgatatg gtcgaatggt tatggtattc attttctgaa
     7021 aatttatgta aaataatgga ttattctaat cgattgctat taactactca cttcaatttt
     7081 aaaataaaaa aagcgcgaac cttcataaga tccgcgcttt gaaattatag attaacaatt
     7141 attgctttac aattttggaa gtaaacatat gctgtccaat taattgtatg gagtaaatcc
     7201 ctgaacttaa atcacgtata gaaattgatt caatattctg tgtgatatta ccagctaaaa
     7261 ctaattttcc ttgcatatca taaatctgat aagagttaaa tacttctttc tccgatttta
     7321 tgattaattg atcaattagt ggatttggaa agaattgaat agcactttca taaatatctt
     7381 gatttccagt tacaataacg tcgatgcaat tggaagttac atcaccttca ttaccataaa
     7441 ctatcaaagc atagctaccc gattgcgttg gagtgaatgt tgaatttgtt tcacccgtta
     7501 tcggtaaatt attattgttg caatcaatcc attgatagga attacctaat tgaggagttg
     7561 taattatccc attgtcaatt tcgagttcaa aattattttc cagtataact acttcaacat
     7621 aaattatact atcacaacct gcttgattag aaatggtttg cgtgtattga ccggcttctc
     7681 catacgattg accattaaat gtaattacat cgtttgtgta aagtgtaata ttcgaaaacg
     7741 tagcacctga aatagttaat tcgagcgtta atgtgctatc acacccaaga ctactttcca
     7801 atgtttgact gtatgtgcct gattcagtat aatttgtgcc atttagatca tacgaaccac
     7861 atgcattaat ttgaatcgtt gcacttacag ctgaacactg cacgaatcga gcaatataac
     7921 catctgaacc tcctgcagaa gttaaattca tcacatcact tgaaggatca aaatcgcatg
     7981 ttccagagaa atttcctatt acactgagtg ttccattagc aggattaatt tgcaaaccat
     8041 ttacatagtc actgcttgta cctccgaagc tataggctga tataaaatca cctgctgaat
     8101 ttagttttgt gataaatgca tcctcataat ttgtgctgga agaagtcata gggaattcgt
     8161 caactggatc aggattaaaa tcggaagtcc ctttaaaatg accacctaca taaatgttac
     8221 catctgaatc tagtttcact cctgtagccg aaccaaccga acttggagca agttctttca
     8281 cgaattgaaa taaacctgaa tttgacaatt ttaacaaaaa tggatggtag cttccagaag
     8341 tatttgtaac cgtttgtgtt gctactaaat ctggatcgaa atcagttgag ccattaaatc
     8401 caccagctag aaataggcta ccatctgtgg caaattcaag agcatatgta aaacaataat
     8461 tagttgaagt gagttgtttt acccaagtga aatttccttg aggagtatat tttgcaatgt
     8521 aagtatcata gtactgacca actaaagaat agttggctgc acttggatca aaatcagttg
     8581 aaccataaaa atatccaacc caataaatat tattatttgc atcaatatta agctccgaac
     8641 aagtggtgta atagcctgga atcgacttag cccaaacgta atctccattt gaagttaact
     8701 tactcatata acaactataa gaagaaacta aacctgttgt tgcaactccc gagttagggt
     8761 caaaatcaac taaatcataa aactgtccag ttgtaataat atttccagaa ccatcaatag
     8821 aaatgtttct tccagactca tatcctgaac ttgctcctcc aaattttttc acccactgaa
     8881 attgaccact tgcagaatat tttacaataa atgcattgta tccaaaaaat gcagtcagat
     8941 tggctgcagt gccctgagga ttaaaattaa cagtaccagt aaattctcct gtacaataaa
     9001 catttccagc aacatccgta gctacatcat aggctaaatc gtttccactt ccaccaaaag
     9061 caaccgacca aacataatca ccattggaat ttaatttaga tatatatgca tctttgagtc
     9121 cattggtact agctggatta ctttctggac ctggatcaaa atcggtaaca ccttcaataa
     9181 agcccgaagt aattattgct ccagaaggat ctttagccat tccaagtgga gtcatattgg
     9241 atgggcctcc aaattgtttt acccaactta aacttaaatt ttgtgagctt gcttcgagga
     9301 cagagagtaa tgccagagca aacggtagaa gtgttttcat gtgtttgatt tttttgtcaa
     9361 aggtgtcctg ttcaccccct ataaatcaat cgtgttaaaa gtggaatttg atctccctta
     9421 aaaaggggat ttttagatta tccccttgac aggcagggtg aattgaatac ttttacccca
     9481 tgcgttttcg attttccttt ttcattattg ggcttctctc atcacttggg gtgaatggtc
     9541 aatcagagga ggaattattg cgtcaattac aagctaccaa gaatgatagt ttgcgctttt
     9601 ttatacaaag ggatttaggt tacctgtttg aatttactga ttcagctaaa gcaatggggt
     9661 attattcgat gaatttacct ttagcccagg aactaaacag caagagatta cttagcatgg
     9721 tttggcttga tttcggtcga gttcaatcca atttcaaaca cacggattca gcccgtaaat
     9781 attatttgaa atcgcttcaa gtggctgagg catcgcctga ttacaaacga gttggagttg
     9841 cacttacaaa tatcggaaat acatatttag ctgagtatcg attcgattta gcagcttcat
     9901 atttattcaa agccatttct gcacaagaaa aggctctgga ttcactgaat ctagcactta
     9961 gttattccac tttaggtgtt ttgtttgaag atgctggtaa atttgataat gctctttttt
    10021 atttaaaaaa atgtaaagag ataagtttat taaatcacaa ttatcaaacg tatttaagtg
    10081 cgagtattac tgaagctgta atttacttga aaaaaggcga tatgcaatcc atgcatttca
    10141 ctattaacaa ggctttggtt atattgtcaa aatataatca tttatattat aaggctagct
    10201 tttatcaaaa tataagtgga ttattctttg attcaaatga tttcttgaag gcaaatcaat
    10261 atgctgattc aagtttatat tatcatacat taaatgatcc tgaatcgatt gatgcggcaa
    10321 cgtatatgtc gaaaggtctt gcattgataa agttgaatca gattgatttg ggtaagacat
    10381 atttggtcaa agccttgaat ttggccatag aagtggaaga ctggcaagtg gctcgggaaa
    10441 tttatttgga attagccgaa atagcagcag ctcggggaga ttggaaatca gcatatgaga
    10501 atcatactaa atatgtggaa tgtaaagaca gtacctatac cgaagaatta gctaagaatg
    10561 cttcagaatt gtctattaaa tttcaatctg aaaagcagca actaaagatt gataaactgg
    10621 cagaagagaa tatgattaat acattacttg ctaagcaaga aaggcgaaaa ggatggttta
    10681 tgcttgctgg gggaagttta cttgctttga tttttattct cttatttatt attcagcgtc
    10741 gaaaaaatca attcattcaa aaagaaaccg aattacagtt agaacaaata aaacgccttg
    10801 aaagcgagaa aaaggtagtt gtacttgatt ctatgcttaa aggagaagaa aatgaacgta
    10861 gcagagtagc taaagactta catgacggtg tcggaagttt actcagtggt gttaaattat
    10921 ccctttcgtc tatgcaagga aacatgatta tccaagaaga acatgcccgg gttttcgaaa
    10981 aatcgctgct acaattagat gatgcaattt cggaaatgcg cagggttgct tacaatatga
    11041 tgccagctgt attgcatcat tctggactca ttgatgcaac gaggagttta gctcattctc
    11101 tttctgaagc atcaggtatt tcaattcatt tcgaacacca ttttttagac gaacgattgc
    11161 ccaatgaata tgagattatt ctttaccgac ttattcagga attgctcaat aacgctctta
    11221 aacattccca agcatcgaat attatcgttc agttaaatgc tcatgaaaat catttatcgc
    11281 ttgttgtaga agataatggt aaaggcttcg acctctcact ttgggaaaaa tcaactggta
    11341 tgggtttcca taatttgaaa aatagagtcg aatatttaaa tggaattatt catgtaacta
    11401 gtagttccga agaagggaca tcgtttttaa tagaatttga actgaatcat gctaaggaag
    11461 cttaatatat tggttgtaga tgaccatccg gttgtgctcg aaggaatgtt gaatattctt
    11521 aaacaattag attttattgc agaaattcat aaagctgaag aagctatttc tgccatgagt
    11581 atcgttcgga gattcgctat cgacgtagtt ataaccgaca tcaatctacc tgaaattaat
    11641 ggaattgaat taggaagaaa aatacatgca gaatttccta gtattaatat tattggcatg
    11701 agtacgtttc atgatccatt gtatgtgggc gaattaattc aagctggcgg aaaaggattt
    11761 atcacaaaaa actcaactgt ttcagaaatc gaatccacta tacgtggagt aatggaagga
    11821 caagttatta ttagtcattt attaaataac caaaaacaag tgtctttccc tgattctcaa
    11881 ggtccaatca ttacgagaag agagaaagaa gtccttgctt taattgccga aggatgtact
    11941 aataaagaaa ttgctgaaaa gctatttgtt agtttgagca cagtagatag tcaccgaaag
    12001 aatttacttg ccaaatttga agtaatgaat acggcatcac ttatcagtaa agctatgaag
    12061 agtggtgttc tctagaaata taaaagcagg cttgaacatt tttttcttat tcaataattg
    12121 tactcttaac ttcctattta attagagagg aagtaaattt atattgttat atgttaaaca
    12181 agtaatattg tatttatttc agacttaatt ttagtttact aaagttttct aaatcttcca
    12241 aagcttgttc aaatgccagg ttttgctcta tcgataattt ttcaaagaaa tggtattcta
    12301 cattcattgt tttagaggtg acggtccttt tccaagtgcc aattacttgt ccattccata
    12361 ttataatgca attgtgtaat gatttaggtt taggttttag gttattccga ttgacaaaaa
    12421 tggccgagcg atctttatat cccatcacgt attcatcgta cataggaagt agcaacatac
    12481 gtgtaggttt gtccgaaatc tctgtttgtt gttgggtgaa atagtataaa gcaccttcta
    12541 attgatatga aatgagttct ttacccaagc tattaattcc tttgcgacat tctgttaaag
    12601 tcaatcccga ccacaccgag taatcgttaa ctgtcgctgg tcctcgactt tggtaaaagc
    12661 gtttggttag ttctatcaaa gcttcatcta tggttaattg tggcacttgt gtaattcttt
    12721 ctgaaattaa agcatatgta tgttgattgc cttttttagc tccgctacat attgttccat
    12781 ttaattcaga atgcatcatg atatagctta atctatgacc acttgcctca atggaatgtt
    12841 gtttaaatac ttcatttaat tcatctcgag ttagttgttt tacaccttgt agagattgta
    12901 ttatgagttc attgcaacga cgaaaaatcg attcattcaa ttcggtttga cgatacatga
    12961 atttatttac ggcatgtaca cgaggagccg ttaatgctaa catccattga atatcttcgg
    13021 ctgttacaaa gtgccaagtt ggacggagaa ggtgggttct taaaattctt ccttcattga
    13081 attcccgttc aatatcactc gccaagcaaa aagctaatcg taagcctaat ccccaaatcg
    13141 tttgagcata ttcctgcgct tgaacggctc caagccacga tactaagtca tgtgctgttg
    13201 tatagcttgg attaagaagc tgttgagcag ctaaacgtaa gtgagcaatg ttggttaggg
    13261 taaatgacat aaaaagaaat tcactcaatt aaattaagtc aaacttattg gttcaaatta
    13321 atttttgata aatgtataaa agtccaattt cctattttgg attactttca gatgatatag
    13381 tccattattc agcgttgaaa tatcaattcg ttttatatta gtggatgaaa ttactttttg
    13441 tccaaaagag gaataaattt caatcatgaa tggttcagat gatagggata aattgatatt
    13501 tataaagtca ttcgctggat ttggaaagaa ttttatttgc gacttttcaa tttggtttaa
    13561 tcctaaacta ctagtttttc tccgagaata aatatggaaa ataccagaag cgtctttttg
    13621 atgataatag atgatttgat tatcggatgt aaggcatgca gcttcaggta caaagccaat
    13681 atttgaatgt ataactgtag gtgtaccaaa tgattctgaa agagaagatc tgacagaaac
    13741 acatatttcg gtatttattg aatttaattg taaacgtgtg taatataatt ccaagccatc
    13801 attacttact tggggcgcat aattaatgta gtttgtgtca ttaatattct ggaaaataaa
    13861 atctgaatta ggagctttat tgaaggtgct atcattcacg tataaggcta ctcccatgtg
    13921 agtttcacaa ggagttccat ttgagcaatt attgaaaaat gcattgcaat agtaaagttc
    13981 gtttccttga tgagttattg ctgcatccat aattagccaa ccaggaatat agctattgaa
    14041 gttgccgtaa acacgagtaa tatctgacac atttccactt gtataatttc ctttgtgcag
    14101 attttcaatt tgaattggat aattgcgaag cgacacccaa taaaaattat tcagcatatc
    14161 caatgaagca accccatcta agtggttggg ggatgagtcg taacaacctc ctactaaacc
    14221 catataagta aaagtgctat catttattcg caaagcataa tacagatttg tatttcctcc
    14281 cgaattcagt gaattaaaaa acaaagtaga tccatcattc gaaataaaag gttccatggc
    14341 atcaaaagtt aagcctgcta tttctacttt cttttcatca tcgaataaag gtgttgcttg
    14401 agctaacagc tgattcagag gcaaaatcaa ataaaataga aaaatggatt ttaaactcat
    14461 caaatcgaaa ttatgggaag attatttaat ttaattactt gctgaattat tcaataaata
    14521 taaagctatt cattcaccaa atgaatacaa aaactttatg attctatgtt taattttcta
    14581 gtgaatttta tcatctactc aacactattc ttgaaatatg taccttttaa tgaattccat
    14641 atttatcttc ttactaaatg ctttttcacc ctagtttcct cttgccaaac aattaaatat
    14701 ctcatttata cccagttcca acaacactca tctaaacatc cttctgctta tctttgccat
    14761 cccaaaatca attgatcagc atggaaacat ctgtttacca gcctcagcat aaaatccgta
    14821 tcgttacggc tgcatccctt ttcgatgggc acgatgctgc tattaacatc atgcgccgta
    14881 ttatccaaag ttctggtgcc gaagtcattc accttggtca cgaccgttcg gtggaagaag
    14941 tcgtgaattg cgccattcaa gaggatgctc aagccattgc catgacctcc taccaaggtg
    15001 gtcacgtgga gtatttcaag tacatgtacg atctgcttca tgaaaaaggc gccggccata
    15061 tccgcatttt aggtggtggt ggcggtacca ttttgccttc cgaaattgag gaattgcagc
    15121 aatacggaat ttcacgccta tatcatccag atgacggacg tagcatggga ttgcaaggca
    15181 tgatcaacga tttaatgtcg cgctgtgatt ttgataccgt aacccaactc aatggagaag
    15241 tcaatcactt ggcagaaaaa gattggaagt ccattgcgag attaatcagc atggctgaaa
    15301 atcatccaga agctgtcggc aattcattcg atcgtattca tgaattagct gctgaaagta
    15361 aatcgcctgt attgggaatt acaggaacag gtggagcagg gaagagttct ttggtagacg
    15421 aattagtacg tcgtttcctc atcgatttcc cagaaaaaca cattggtgtt atttcggttg
    15481 atccatctaa acgcaaaacg ggcggggcat tgctgggcga tcgtattcgt atgaatgcca
    15541 ttcgcaatcc acgtgtgtac atgcgctctt tggctacacg tcaaagtaac ttggctctta
    15601 gtaagcacgt gaaagaggca gttgctgtat tgaaagcggc taactatgac cttattattc
    15661 ttgaaacatc cggaattgga caaagtgata ccgaaatctt agatcacagc gatgtgagct
    15721 tgtacgtgat gacacccgaa tacggcgctg ccacgcaact cgagaagatt gatatgctcg
    15781 attttgctga cctcatcgcc ctcaataaat tcgataaacg tggtgcttta gatgctttgc
    15841 gtgatgtgaa gaaacagtat cagcgtaacc ataatttgtg ggatacaccg ccagaagaaa
    15901 tgccggtttt cggtaccatc gcatctcaat tcaacgatcc gggtatgaac aacttgtatc
    15961 gtcgcatcat ggatactttg gttgaacgaa ctggagcaga tttgaaatca ggttacacag
    16021 taacccagga aatgagcgag aaggtgtatg ttattccacc caatcgtacg cgttacctta
    16081 gtgaaatcac cgaaaacaat cggaattaca acagttgggt agagaagcaa tccgaagttg
    16141 ccgaaaaatt atatgcgatc aataaaacga tggacacgat gaagtccacc gtgaatgcag
    16201 atattttgaa agtgcttcaa gcggaattcg atcgtgtgaa attggagctc gatcctaaaa
    16261 attggttact catcgaacaa tgggaagcca aacgccaaag ctacaaggat gaatttttca
    16321 tctttaaagt gcgcgacaaa gaactcaaga ttgccacgca ttcggtgagt ctttctcaaa
    16381 gccgaattcc taaagtagta actccggcat acaagaattg gggtggaatt ttacgttggt
    16441 cgcttaccga aaatgtaccg ggcgaatttc catttgccgc gggtatttat ccgttcaaac
    16501 gcgaaggaga agatccaacc cgaatgtttg ctggagaagg tggaccagaa cgtaccaatc
    16561 gtcgtttcca ttacgtgagc aaaggcatgc cagcagctcg tttgtcaacg gcattcgatt
    16621 cggttacgct ctacggaaat gacccagatc ttcgtcccga tatttatggg aaaattggga
    16681 attcgggtgt gagtatttgc tgtctcgatg acgcgaaaaa actctattct ggtttcgatt
    16741 tatgtgctcc aagcacttcg gtttcgatga ccattaacgg tccggctccg atgctgttag
    16801 gctttttcat gaatgctgcc atcgatcagc aatgcgaggt ttatattcgt caaaatggtt
    16861 tgctcgatga agtaaatgct aaaatcgatg cactctttgc tgctaaagga atgaagcgtc
    16921 cgatgtacca aggcgaactt ccagaaggca ataacggact aggtttgtta ctcctcggcg
    16981 taacgggcga tcaggtgcta cctgccgatg tgtatgctgg tatcaaagcc aaaacattga
    17041 gcgttgttcg cggaacggtg caagcagata ttttgaaaga agatcaggcg caaaacacct
    17101 gtattttcag taccgaattc gcgcttcgta tgatgggcga tattcaacaa tatttcatca
    17161 gcgagaaaat tcgcaatttc tatagcgttt ctatttcggg ttaccacatt gcagaagccg
    17221 gtgcgaatcc catcacacaa ttggccttca cgctttcgaa tggtttcacg ttcgtggaat
    17281 attacctcag tcgcggaatg catatcgatg attttgctcc gaatttgagc ttcttcttca
    17341 gtaacggaat cgatccagaa tatgcggtta ttggtcgtgt ggctcgtcgt atttgggcaa
    17401 aagccatgaa attgaaatac ggtggaaacg aacgcagtca gatgttgaaa taccacatcc
    17461 aaacttcagg gcgttccctg catgcacagg aaattgattt caatgatatt cgtacaacgc
    17521 ttcaagcttt gtatgcgata tacgacaact gcaactcgct gcacaccaat gcctacgatg
    17581 aagcgattac tacgccgaca gaagaatcag tacgtcgtgc catggccatt cagctcatca
    17641 tcaacaaaga gcttggttta gcgaaaaacg aaaacccgat tcaaggttcg ttcatcattg
    17701 aagaattgac cgatttggta gaagaagcca tctacacaga attcgatcgt attaccgagc
    17761 gcggaggcgt gttaggtgcg atggaaacca tgtatcagcg cagtaagatt caggaagagt
    17821 cgatgtacta tgaaatgcaa aaacataccg gcgaattccc gattattggt gtgaatactt
    17881 tcctcagttc caaaggttct ccaacggtat tgccgaaaga agttattcgt gcatcggaag
    17941 aagaaaagca gtatcaaatc gatatgctca aagaccttca cacccgcaac gcaagccgta
    18001 tcgatgagct attacgcaag ctccaagagg ctgcagtcct caaccaaaac atcttcgaac
    18061 aattgatgga agtgagcaaa tacgcatcac tcggtcaaat taccagttcc ttgtttgagg
    18121 taggagggca gtacaggaga aatatgtaga tggtgtgctg gtgtgctagc gtgttgatgt
    18181 gctggtgatt tttgatgtgc tcccttcgac aggctcaggg agcgagtacc gcacgctgag
    18241 cctgtcgaag cgtgggcttg cctccaaagg agtcgggctc gtgtatgtcg aagctttagc
    18301 gaaggcatgt gctgttagct tttcgcgact tgtccgtatg ggcaatccct taggaggatt
    18361 taacggtagt gtgcaacata ctaatcctac taaatagatt tagattttat taatgtgtaa
    18421 tttcttctat gattcgatgg aacctctcat ttaaagtttt aatctttgat acatcttacc
    18481 gaaaatccat ttttcttatt tgtattatta taagctgcta catcatagtt aaaaacaaga
    18541 tttcggtacc aagcttctga taatgaaaac tctgaattac tccaccacat tccactataa
    18601 acaatgtgga gaaatgttga aaaattacca atgacttgga tataacctcc aggaaggcct
    18661 gtgaagagac tttcattgtt tgcccctgta ttcggagcat tccagccagt tattgctttc
    18721 attttacctc cggctgtgct aaatcctcct agataatctg ttagtaatgt ccattccgta
    18781 tcagagggta catgccagcc atcaggacat acattacgag gatctgcaac tgtaaaccag
    18841 ttataaagtt tcccataaat agagccgtat agaatactat tgttgtaatt acaccaagca
    18901 ggagaactta attgggacca tgtaagactt tcagttatat ttggtatcag tgtaccatcc
    18961 gcaaaatgaa ttgtttttag gttctctgcc atccaccatt ggtcacctat taaaacggtt
    19021 gaatacagat tcccatcaat atcggatacc gatccaaaca ttgtagaaca tgtagcaatc
    19081 attccggcat ccacccccga tatacccact tgaacatcta tcaaattact cacattaaca
    19141 gaatcggaat tggaaatatc ataactccga attgaatatg ttcccatttg gcaggtctga
    19201 atgctgaacg ttccattatc caaaataaaa attcgtccat tcattttaac gtaacccaaa
    19261 tccactggct gaccttcaca attaactact gttcctgtaa taggataacg gttttctaag
    19321 ttcgctgtta aaacaataga tagaggttgc gtttcactgg caatggtttc ggaatgtgcc
    19381 aaattccagt cgttggtagt gctacaggtt aaatacacgt ttagcgtaag ttgctgattt
    19441 ttcggcactc ttcctgtaaa aacgccttct gagttggtgt aagtaacacc tgttcccaac
    19501 gtttgagtaa ccacattcac ctgcgcatct gatacaggtg ttccacttac cgaatttatt
    19561 gttccattaa aatcgttgaa attctgaggc acatcgtaat tccaccaagt aaagtgattt
    19621 gcttgtccta catattgaga atcttgcttt atggcttctc cttcgtgttt ccaataacca
    19681 agtgattcat caaaactcca ccaatcaata gtttcggggg cttcggcttg caaagcagta
    19741 ggaatattaa aagtgagcga agctgaaact ccatttgcca tttgcaattc attcatgctg
    19801 gtatctctca gttcaatact tgccatgcca aatgagcgta acaaacgcaa cgaatcattc
    19861 atgccaccca acaattcacc aggcatttgg tcaaacattt cgggagaagt tggatctaat
    19921 gcttgtgcat agacatttac agttccagta taaggttgtc catttaattg aatagcatt
//
LOCUS       DAOOQW010000027        19114 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_3499_length_19114_cov_6.120835, whole genome shotgun sequence.
ACCESSION   DAOOQW010000027 DAOOQW010000000
VERSION     DAOOQW010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 19114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19114
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3499_length_19114_cov_6.120835"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..5543
                     /locus_tag="PK281_03150"
     CDS             <1..5543
                     /locus_tag="PK281_03150"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOY48369.1"
                     /translation="SNGSCTPSQSTVTITVDNNPLSPNAGIDQLICTSTTTLSATAAT
                     GGSWSLVSGSGNIVSANTAITSVTGMTAGVNVFRWTIPGGSCGSVSDDVSIVVQNPPS
                     TANAGSDQTICSSTTTLAASTPAVGNGTWTVVSGFATFSNANSPTSDVSGLNSGTTVL
                     RWTITNGVCTSTSDDVSIFVTSAPATPSAGLDQTICSSTTSLSASPSGGIWTLSSGSG
                     TIVNASSATTSVTGLTTGTSVFRWTIDGGTCPDVWDEVSITIQALPTTSAAGQDQTIC
                     STSATLSANAPSIGSGQWSLISGSGTFANASLPNSTISGLGTGTNVLRWTISNGVCQA
                     STDDVTITVDANPVTPSAGIDQTICSSSTQLSASPAGGTWTLVSGTGTFQNATSPTTQ
                     VSGLSTGVNILRWTVSNGSCADASDDVSITVQAAPTVSNAGVDQQVCATSTTLSGNIP
                     TVGSGVWTVVSGTATFVNATSANTAVSGLSAGVNVLRWTISNGVCSSSVDEVTITVDS
                     NPVSAFAGNDQSICSSTATLAATVATGGLWTLVSGSGTIVSATSANSSVTGLIVGTSV
                     FRWTVSSGTCPAVSDEVSINVLNPPTTANAGSDQTICLSATTLAANTPSSGNGVWTVV
                     SGSGFFANATQPNSNVSGLSTGVNILRWSISNGVCSPSTDEVSITVLQAPSNVQAGSD
                     QIICGSSATLSASAPSQGSGHWTVVSGSGVFSNSNSETSGVTGISAGQNVFRWTVTNG
                     SCASASDEVSVQSFAQPTTANAGSDQTICTNSTTLQGNFATIGTGTWTLISGSGVILN
                     TSTPQTQVSNLTVGTHVFRWSISNGVCSPSTDEVTIQVLAPLVSDAGSDQDVCSSSST
                     FQATLPSGATGLWTLISGAGIIQTPTAANSQISNLGIGANVFQWSVNNGVCPVATSTV
                     TINRFADPSAANAGIDFQTCANSANLAATTPSIGTGFWSVVTGSGTFSNPTLSNSAVT
                     GLSAGINKLEWAISNGPCSIKRDTVIVTVDQNPTNAFAGSDQQLCTANSSFSATPASS
                     GTGSWTVISGSGTIANANLATSAVTNLGAGANVFRWTVVSGTCTTFDEVILTRFLPPT
                     TAVAGTNLIICSSSATLNANAPTIGTGLWTVLSGSGTFAQASNAGTTISNLGIGANVL
                     SWTISNGVCPASVDQVTIDRQESPTTANAGLDQQICSDQIQLSGNVPTVGNGVWSLVS
                     GTGTLENALSPTCVVSGLGAGANVFQWSTLNGLCPASTDQVTITRDLAPSQADAGNDT
                     STCLTSIQLQAINPSIGTGVWSIVSGSGSLGNATSAQTNLTSLNPGITEVLWSVSNGV
                     CPSTSESIQITVYAAPQTAFAGIDEVICSDSIFLVGNTSNGSSYLWEVISGSAVLADD
                     TAAQTALTSIAAGNSLIRYTITNGPCSSSDSVAILRYENPDLANAGLDSTFCSDVFVN
                     QATQPLIGQGHWFSFDPNVSFSDSSAANTSVNLPAGISTLVWIVENGICPALSDTIVL
                     QRDVPTELASAMSDTNVCAANLNIFAQSPTQAIGYWELISGDGVFIDSTVAQTELGMS
                     AEGENVIVWTLEEGSCLSSDTVTIAFNYAPFPVSAGADRSVCGLTTDLEGVSSGIGSG
                     LWTFLNSDGTLSDPSDPIAFLTVDYTATRELIWTVTNGFCAVSDTVTLDFLDQPISDA
                     GLDLIGCLNDTTALQAAIPSLGIGTWTVLTPGGNVFEPGFQNSNFTATEQGNFYLQWT
                     VVNGICRDSSFTIVTIYGPDDPECSGPDVVFIPEGFSPNGDGVLDKLIIVKPQTKRVS
                     LEVFDRYGILVYSSNDYQNDWDGTAQAGTILHGNQLPESTYYYLIQIEGELGVRKGYI
                     TLWR"
     gene            complement(5540..6859)
                     /locus_tag="PK281_03155"
     CDS             complement(5540..6859)
                     /locus_tag="PK281_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48370.1"
                     /translation="MRHERFLSLVATEKYAEAETTLTSTKKAKKKKLKLLFLLEMGYV
                     TFAQGKYSESSKYFAEADYLIEDYKKNLGYEALAIIVNPGVKPYKAEDFEVVMLHYFN
                     AMNYLKQRDFENALVECRRMNLSLITMAEKLKDKPKYKEDAFAHLLMGLTYEGSGDIN
                     NAFIAYRNAYDIYAGDYTSFFEMKIPEQLKKDLVRTAAANGFRNEEELYRKKFGYSST
                     DTLPKANKTELVLFWNNGLGPYKEQWSIDFNLVKQGDQFVFMNSGLGYSFPFPVSSVS
                     QQDAKNLSQLSVYRIAFPRYVHRPMMYRSARASLGGANYPFEIAENIDAIAFKSLNDR
                     FAREMGSALIRFAVKKAAEVALRKENKDAATVLSIANAISEQADTRNWQTLPNQISYT
                     RIPLIPGKNEVLFYATAAQGGRTRCDTIRIDARKGQVYYEIVNTVNR"
     gene            complement(6961..7554)
                     /locus_tag="PK281_03160"
     CDS             complement(6961..7554)
                     /locus_tag="PK281_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010520381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HOY48371.1"
                     /translation="MRTSIKSLIGLALISTIGFSSCARRTVTRVAPDKVIDLSGRWNE
                     TDARLAAETLTNQALTGDWLGNFTNEKKKKPVVIVGFVRNNSHEHMDTEIFTKDIEKA
                     FIKSTMIRLVAAGDKRTEIRGERADQQDFSSAETMKKFGKEVGADFMMQGTVKSIVDG
                     YGKEKTTYWQIDLELTNIETNEIVWIGDYKGKKYIVN"
     gene            complement(7572..7979)
                     /locus_tag="PK281_03165"
     CDS             complement(7572..7979)
                     /locus_tag="PK281_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48372.1"
                     /translation="MTLSRLAPSFVFLALFLASCADEEPIVPEVDPKDKFIGTWNVKE
                     EIGGSVTGNYPSTVTKDSTNTSRILIYNIFNLGAAQRVQALVANNTLDISQQSITGIS
                     IAGSGTYSGTGFILNYTSDDGSGAQTVKATYTK"
     gene            complement(8068..9576)
                     /locus_tag="PK281_03170"
     CDS             complement(8068..9576)
                     /locus_tag="PK281_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015481604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HOY48373.1"
                     /translation="MGLKSFFAKPLASIIARQVRNWSNNPIVTQEKVFQQLIAGARDT
                     AFGKDHDFATIRNYDDFKTKVPVRDYEGLKTYIDRATKGEANVLWKGKPIYFCKTSGT
                     TSGTKYIPITSDSISNHIESARNALLLYIHETGNAKFVDGKLIFLSGSPVLSEISGIP
                     LGRLSGIVNHHVPQYLRRNQMPSFETNCIEDWETKVRKIAEETISEDMTLISGIPSWV
                     QMYFEILQEKSGGKTMKDLFPNFSLFVYGGVNFEPYRSRFEKMIGKSIDSVELFPASE
                     GFIAYQDKQPSQGLLLILDSGIFYEFIPVDEFFSENPTRISLKEVKVDVNYVLILNTN
                     AGMWGYNIGDTVKFVSTNPYRLVVTGRIKHFTSAFGEHVIAEEVEYALKEASLGEVVE
                     VVEFTVAPQVSPETGLPYHEWFVEFSVKPKDPERFRMKIDTALQKKNIYYKDLITGNV
                     LKPLVLRSLEKDSFVKYMKSQGKLGGQNKVPRLSNDRKIAEELSNLIDQSWS"
     gene            9732..10805
                     /locus_tag="PK281_03175"
     CDS             9732..10805
                     /locus_tag="PK281_03175"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOY48374.1"
                     /translation="MKPKLDIIQFFRAVAALLVCFFHMKGILKQGEWGKFLFGNGSVG
                     VPMFFMISGFIMYHTTRMVQPNWKNVKAFLLKRLIRILPLYYLMTLLYVLLLGNAHYY
                     FIENPSFLLPTFFFFPTYVNLIGPSYGMPPLAVGWSLNYEIYFYLLVGISMLFVKNRW
                     FMLCGWLLGTTILIPLLSKGYVMMSLSECYGYTAVYLNLMTNPVLLFFIAGIVIGALY
                     TSSVVPPSLFWADASAQLAVVVFILSYFHLIQLLPGWYNHLLTCGFLLVALLQRNKIK
                     PYSIPKSLLIIGNCSFSLYLVHPIVLTYLPKVLRTLGVQAVLEGWVYFIGVLLVIVAI
                     SYLSYVWIEQRLSLKIQSLVLKE"
     gene            complement(10824..10991)
                     /locus_tag="PK281_03180"
     CDS             complement(10824..10991)
                     /locus_tag="PK281_03180"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HOY48375.1"
                     /translation="MLAVDPHFPINNAKNIVGLRNQIIHAYDSISDETIWGILINHLP
                     KLKAEINSLIH"
     gene            complement(11141..11452)
                     /locus_tag="PK281_03185"
     CDS             complement(11141..11452)
                     /locus_tag="PK281_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011709600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOY48376.1"
                     /translation="MILIDANREKLYSLCEKHKVDELYLFGSILTDRFIDSSDVDILV
                     QFGKVELEQYADNYFDFKEELERLFNRSVDLVENQSIKNPVFRKVVDREKQLVYERKD
                     T"
     gene            complement(11537..13129)
                     /locus_tag="PK281_03190"
     CDS             complement(11537..13129)
                     /locus_tag="PK281_03190"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48377.1"
                     /translation="MKPIILFLALLTNHFGQAQKKLWLLKIGEDSVIRVRPENDFYDR
                     EDSFNGTYWAEGSRTRFNFPYGTRQFRTGLYRGWNTSNEFQFMDAQGNHLNDSAIWAT
                     GQMQDFKRIGIWRKYHLNGKVALEREYCNGTPCGKISLFYENGQVKSSGEVDTNAYPH
                     GTWKAWFENGKPKWEIEFEHGMTKGWSAHYNESGLLCKVHFISNHWANNYDELLMYYP
                     ETGKMKARMGLRNSIPIIIDAWDENGNQTASNGNGFINGEYQQMERGKLEAIIINGEL
                     RSNCRRYYDTEGKKLRSDVREDSLIPGLMHSTGYYENGALNFTLNYQFSDPNKRDGIT
                     REGPSTYYHPNGQLSHRCQFEKDQVVGLFQQWNSNGILLAEVMFSAKYVPNPTYLKEI
                     DDVYLSVAGVPDGSWKLFTDQGKLISESVYKNGLRHGVWKTWHTNGQLKSQREYRNDE
                     PINDEIHFDINGKKIHEKKKKCPNENWGSSYTCSEHGLVSVTDDIGVCSNCGKGTSSG
                     MYGLCAHCACVSGRCQMCGRKY"
     gene            complement(13150..14529)
                     /locus_tag="PK281_03195"
     CDS             complement(13150..14529)
                     /locus_tag="PK281_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008992219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOY48378.1"
                     /translation="MLHIRRKGLTLLEHWRNKKGRKPLVLRGARQVGKTTLVRDFSKS
                     YSQFVSLNLELAADKKLFSDDMDVVALMQRLTIRESIKPDWENTLLFIDEIQEVPDAI
                     QLLRYFYEQFPELSVIAAGSLLEFALGSVRSFPVGRVEFQYLHPLSFEEFLIAMDKQQ
                     AIDAMKQLPVPAYAVEELHKQFNLYCLLGGMPEVLARYREDGSVFNIQQIYESIWKSY
                     QNDVEKYGGNKSAKQMIRFVLNNAPASFDQRITFQNFGQSTYRTREIGEAFRQLHDTR
                     LLRLIYPTTDVKTPLQPDYRKSPRIQFLDCGLLVNRLGILAELIGLEDLSDAARGALI
                     PHCVFQERIAIQDYTDSSPFFWVREKSQSSAEVDLIIQRGSLIIPVEIKSGSIGKLRS
                     LHAFMDQTPSTLAIRLHQGKASIDEVKTTSGKVYSLLNLPYFLAASLESYIENWRNED
                     GKSWKMISY"
     gene            complement(14636..17551)
                     /locus_tag="PK281_03200"
     CDS             complement(14636..17551)
                     /locus_tag="PK281_03200"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HOY48379.1"
                     /translation="MRQLIFLYLSLFVSAAFAQYTPVKLVNPMVGTGGHGHTFPGATV
                     PFGMVQLSPDTRVDGSWDGCGGYHYSDSLIYGFSHTHLSGTGCCDYGDILLMPLMNRP
                     DLTKKHFASSFSHKAEQAGAGWYEVVLRDQQIRAELTTSTRVGFHRYTFPGKNGFVLL
                     DLLHRDELLEGKVEKVDDHTIRGMRRSKAWAQDQVIYFEIAFSQKISVIEESSNSTQG
                     DLRTAIQFENMSSNVLEVKVGVSFSSFEGAHLNLTSEATNVNFDLAKERAIKYWETEL
                     NRIQIKGGTTEQQKIFYTALYHTMIQPNTVSDVDGSYRGMDKKVHKAEGFTYYSVFSL
                     WDTFRAAHPLYAILYPERTRDFIQTFLRMYQEGGRLPVWELASNETDCMIGYHSVSVM
                     VDAYFKGIKGFDTQLALEAMQKSATWNHLGLPAYMDHGFLSVDDEHESVSKTLEYAYD
                     DWCIARFAEELKKPAVYSTYAARSKNWKNLFESKSGFMRPKKNGAWLSPFDPREVNNH
                     FTEANSWQYSFFVPQDIYGLAQAHGGQDILIKRLTGLFEADSKTTGRTQSDITGLIGQ
                     YAHGNEPSHHMAWMFSAIGEPRLTQQYVRRILKEMYHNAPDGLSGNEDCGQMSAWYVF
                     AAMGFYPVCPGNPNYVVGAPIFDEIKITPVNGKLFSIKAPGASKNEYTTAVEGKQNYW
                     FNHSSIINGESISLSMGKVAPQKQTLQQIPFTYDWSAIKTVQAPVFSTSQQVFRDSVL
                     VKIESNAPGAKIYYTLNGEDPSPLSPLATDPIIIQNSCQLKAIVIKDGISSPVNTAAY
                     NKIPHNWTVKISSTYNPQYSAGGDQGIIDGLFADENWRKGGWQGYQGQDFECVVDLQK
                     TTEISTIGASFLQDSRSWILFPTEVRFEYSLDGISFTPFVTDSNFIDPKDTKVQIQKF
                     VEERSFVANEHNEPMTIPQPTTMRYIRVKAKNFGILPEWHQGAGGEAFIFIDEIWAK"
     gene            complement(17642..18331)
                     /locus_tag="PK281_03205"
     CDS             complement(17642..18331)
                     /locus_tag="PK281_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48380.1"
                     /translation="MKYTRIYFILLLAIASISSCKEDEEPISQQTEFSFTSNDSLIFE
                     HSGTLPYSVAVQCASSKELGASFETVTGGFDVGSQLTIPSNESRNFNVSFNQTFATPG
                     AYPADLSVSIFNENSTPKTKTVYFVYRPNCGYAFRNYTIGQITYISNGSLLNKNITCT
                     YNSSGQLVVSGLTTFDVTLNFNCDNNTVTMVPLVINGFLTTGNGQIEGTEIALQMYSD
                     GELHSNSRIKF"
     gene            complement(18365..19033)
                     /locus_tag="PK281_03210"
     CDS             complement(18365..19033)
                     /locus_tag="PK281_03210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48381.1"
                     /translation="MSSCSINHIADFNGNQPELLEEKKDVRITTGINLNLTNRPIGGE
                     LNVSYAVSSRWFVTSGVRTGGFIFPTGYGYGSDFRSFTAGGGYYTLTQKGNNHQVTLR
                     YVRGDEKQYEVDGTNYYWRHWHKAHLYNGFDFSYSAAFVNASSKLGYSFRFNYLTTLQ
                     SQHIPRLGVYFNYTHNTHRRFLLAFTAGFLTELNNDFLSTNYILFRPTITYCFNATRK
                     KKSH"
BASE COUNT         5353 a         3952 c         3905 g         5904 t
ORIGIN      
        1 tctcaaatgg tagttgtaca ccatctcaaa gcaccgttac tattacggtt gataataatc
       61 cactttctcc taatgctgga atagatcagc ttatttgtac gagcactaca actctgtcgg
      121 ctacagcagc aactggcgga agttggagtc ttgtaagtgg atcgggaaac atagttagcg
      181 caaacaccgc tattacaagc gtaacgggca tgacggcagg ggtgaatgta tttcgatgga
      241 caatacctgg tggttcttgt ggatctgttt cggatgacgt atcaattgtt gttcaaaatc
      301 caccttctac tgcaaatgcc ggttccgacc aaacaatctg ttcatccaca actactcttg
      361 ctgcaagcac acctgctgta ggaaatggaa catggaccgt tgtgagtggt ttcgctactt
      421 tttcaaatgc aaattctcct accagcgatg tttcgggact aaattcggga acaactgttt
      481 tacgctggac catcaccaat ggtgtttgta cttccacatc cgatgatgtg agcattttcg
      541 ttacatccgc gccagcaact ccttctgccg gtctggatca aacgatttgt tcatccacaa
      601 caagcctttc agctagtcct tctggtggaa tctggacatt aagcagtggc tcgggaacaa
      661 tcgtaaatgc atcatctgca accacatcgg taacgggatt gactaccgga accagcgttt
      721 tccgttggac gattgatgga ggaacttgtc cggatgtttg ggacgaagtt tcaatcacca
      781 ttcaagccct tccaaccaca tcagccgcag gccaagatca aaccatttgt tcaacatcag
      841 ccacgctatc ggctaatgca ccaagtattg gatcaggtca atggtcactt atttccggca
      901 gcggaacatt tgcaaatgca tctttgccta attcgacaat ttcgggatta ggaacaggta
      961 caaatgtgct acgctggacc atcagcaacg gcgtttgcca agcttcgacc gatgatgtta
     1021 ccataacagt tgatgcaaat ccggttacac catcagctgg aatcgatcaa acgatttgtt
     1081 cttcttcaac tcaattatct gctagtcctg ctggtggaac ttggacatta gtaagcggaa
     1141 ccggaacttt ccaaaatgca acatcgccaa cgactcaagt tagcggatta tcaacgggtg
     1201 tgaatatttt aagatggact gttagcaacg gatcttgtgc agatgcttcg gatgatgtat
     1261 ccattactgt tcaggcagct ccaactgttt caaatgcagg agttgatcaa caagtatgtg
     1321 caacttcaac gacactttcg ggaaatattc caacagttgg ttcaggtgtt tggacggtag
     1381 tttcaggaac agcaactttc gttaacgcaa cttcagcaaa cacggctgta tcaggcttgt
     1441 ctgctggtgt aaatgtgtta cgctggacga tcagtaacgg tgtttgttct tcttctgttg
     1501 atgaggtaac tattactgtt gatagcaatc cggttagcgc atttgcaggt aatgaccaat
     1561 caatctgctc ttcaaccgcc actttagctg caactgttgc aactggaggt ttgtggacat
     1621 tagtttccgg ttccgggaca attgtaagtg ctacttcagc caattcttca gtaacaggac
     1681 taatagttgg cactagcgta tttcgttgga cagtttcttc tggaacgtgt ccggcggttt
     1741 cggatgaagt cagcatcaac gttttaaatc ctccaaccac tgcgaatgca ggtagtgatc
     1801 aaacgatttg tttgtcggct actacattag ctgccaatac tccgagctca ggaaatgggg
     1861 tttggacagt tgtttccggc tcgggatttt ttgccaatgc aacacagcct aactcaaacg
     1921 tatcgggact ttcaactgga gtaaatattt tacgctggtc gataagtaat ggagtatgct
     1981 ccccatcaac cgatgaagtt agcattaccg ttttacaagc accaagtaat gttcaagcag
     2041 gatctgatca gattatttgt ggctcaagtg caacactatc ggcaagtgca ccttcacaag
     2101 gttcaggaca ttggacggtt gtatcaggtt caggagtatt tagcaattca aactctgaaa
     2161 catcaggtgt aacgggaatt tcggcaggac aaaatgtatt tcgttggact gtcacaaatg
     2221 gcagctgtgc ttcggcttct gatgaagtta gtgttcagtc gtttgctcaa ccaactacag
     2281 caaatgcagg atcagatcaa acaatctgta caaattctac cacacttcaa gggaattttg
     2341 ccacgattgg aactggaact tggacgctaa tttcgggaag tggtgtaatc ctcaacacat
     2401 ccacacctca aacacaggtc agcaatctaa ctgttggaac acatgtgttt aggtggagca
     2461 ttagtaatgg agtttgttca ccatcaaccg atgaagtaac cattcaagtt cttgctcctt
     2521 tagtttctga tgcaggtagt gatcaggatg tttgtagttc ttctagcact ttccaagcga
     2581 ccttaccatc gggagcaact ggattatgga cattgatttc cggtgctgga ataattcaaa
     2641 cgcctacagc agcaaactca caaatcagca atcttggaat cggagcaaat gtgtttcaat
     2701 ggagcgttaa caatggtgtt tgtcctgttg cgacatcaac cgttactatc aaccgctttg
     2761 ctgatccatc cgctgcaaat gctgggattg atttccaaac ctgtgctaat tcagccaatc
     2821 tagctgctac aactccaagt atcggtacgg gattttggtc ggtcgttact ggttcgggaa
     2881 cgttttcaaa tccaacctta tcgaacagtg ccgtaacagg actttctgcc ggaatcaata
     2941 agctcgaatg ggctatttcg aacggaccgt gttcgataaa gcgcgatacg gttattgtta
     3001 ctgtagatca gaatcctact aatgcgtttg caggaagcga ccaacagctt tgtacagcta
     3061 acagtagttt ttcagccaca ccggcatcat ctggaacggg aagttggact gttatttctg
     3121 gttcaggaac aattgcgaat gcgaatctag ccacttcggc cgtgacaaat cttggagctg
     3181 gggctaatgt gtttcgctgg actgtagtaa gtggaacctg taccactttc gatgaagtta
     3241 ttctaactcg attcttgccg cctacgactg ctgtagctgg gacaaacctg attatctgtt
     3301 catcatcagc cacgctaaat gcaaacgcac caacgatcgg aactggactt tggacagtct
     3361 tatcaggctc tggaactttt gctcaagcat cgaatgctgg tacaacaatc agcaatttag
     3421 gaataggtgc aaatgtatta tcctggacaa ttagcaatgg agtttgtccg gcttctgtag
     3481 atcaagttac tatagaccgt caggaatctc caacaacggc aaatgctggg ttggatcaac
     3541 aaatttgttc tgatcaaatt cagttatcgg gtaatgttcc tacagtagga aatggtgttt
     3601 ggtcgcttgt aagtggaacg ggaaccttag aaaatgcttt atctccaacg tgtgtagtta
     3661 gtggtttggg tgctggagca aatgtgtttc aatggtcaac actcaatggt ttatgtccgg
     3721 cttctaccga tcaggtaacg attacgcgcg atttagctcc ttctcaggct gatgctggta
     3781 acgatacaag cacatgttta acttctattc aacttcaggc aataaatcca agcatcggaa
     3841 caggagtttg gtcgattgta tcgggaagtg gtagtcttgg aaatgctaca tcagcacaaa
     3901 caaatctcac cagtttaaat ccaggtatta cggaagtgct ttggtctgtg agcaatgggg
     3961 tttgtccatc gacttcagaa tcaattcaaa tcacggtata tgccgcacca caaacggcat
     4021 ttgctggcat tgatgaagta atatgttccg acagtatttt tctagttgga aatacatcta
     4081 acggcagttc ttatttgtgg gaagtaataa gtggaagtgc cgttttagct gatgatactg
     4141 ctgctcaaac agcattaaca tcaattgctg caggcaattc gcttattcga tatacaatta
     4201 caaatggccc ttgtagctct tccgattcgg ttgcaatttt acgttacgaa aatcccgact
     4261 tggcaaatgc tggtttggat agtacatttt gttcagatgt gtttgttaat caagccactc
     4321 aacctttaat tggacaagga cattggtttt cgttcgatcc gaatgttagt ttctcagata
     4381 gttctgctgc aaatacttct gtcaatttac cggcaggtat ttcaacgctt gtttggatag
     4441 tagaaaacgg tatttgtcca gcattgtcgg atacaattgt gcttcaacgc gatgtgccaa
     4501 ccgaattagc atctgccatg tccgatacca atgtgtgtgc tgctaacctg aacatttttg
     4561 ctcaatctcc gactcaagca attggatatt gggaattgat ttcgggtgat ggtgttttca
     4621 ttgactccac agtggctcaa actgaattgg gcatgtcggc cgaaggtgaa aatgtgattg
     4681 tttggacttt agaagaagga agttgcctta gcagtgatac cgttacaata gctttcaact
     4741 atgcaccatt cccagttagc gctggcgcag atcgttccgt ttgtggatta actaccgatt
     4801 tagaaggtgt gtcatcaggt attggttctg gattatggac tttcttgaat tccgatggga
     4861 cattatcaga cccaagcgat ccgattgcat ttttaacggt tgattacacc gctacacgtg
     4921 agttaatttg gaccgtcacc aacggatttt gtgcagtgag tgatactgta acactcgatt
     4981 ttctcgatca acctatttcg gatgctggac ttgatttaat tggttgttta aatgatacaa
     5041 cagcccttca agctgccatt ccttctttgg gaataggaac ttggactgtt ttaactccag
     5101 gcggaaacgt ttttgaacca ggtttccaga acagtaattt tacagccaca gaacagggta
     5161 atttttatct acaatggacg gttgtaaatg gtatttgtcg tgactcttca ttcacgattg
     5221 tgaccatata tggtccagat gatcctgaat gtagcggtcc agatgtggtt tttattcctg
     5281 aaggattctc ccctaacgga gatggagtat tagataaact gataattgtt aagccgcaaa
     5341 cgaaacgtgt ttcactcgaa gttttcgacc gatatggaat actggtttat agcagcaatg
     5401 attatcaaaa cgattgggat ggaacagctc aggcaggaac gattttgcat ggcaatcagt
     5461 tacccgagag tacgtattat tatttgatac agatagaagg ggaactaggc gttcgtaaag
     5521 gatatatcac cctttggcgt taacgattaa cagtatttac aatttcgtag taaacctgtc
     5581 cctttcgtgc atcaatacga attgtatcgc atcgcgtgcg tccaccttga gctgcagttg
     5641 cataaaaaag cacctcattt ttacccggaa tcaaaggaat gcgggtataa gaaatttgat
     5701 ttggtaaagt ttgccagttt cgtgtatcgg cttgttctga tatagcattg gcaatgctta
     5761 aaacggttgc ggcatctttg ttttcttttc gaagagctac ttcagccgcc ttcttaacag
     5821 caaaacggat taatgcactt cccatttcac gagcaaatcg atcatttaaa cttttgaagg
     5881 caattgcatc aatgttttcg gctatttcga atggataatt tgctccacct aaactcgctc
     5941 tagccgaacg atacatcatt ggacgatgca catatcgagg gaatgcaatt cggtaaacac
     6001 tcaattgact aaggttttta gcatcttgct gactcacgct actcacagga aatggaaagc
     6061 tatatcctaa tccagaattc ataaatacaa actggtcacc ttgtttcacc aagttaaaat
     6121 cgattgacca ttgttctttg tatggcccaa gaccattatt ccaaaataga acgagttcgg
     6181 ttttgttggc tttgggaagc gtatcggtag aagagtaacc aaatttcttg cggtaaagct
     6241 cttcttcatt acggaaacca tttgcagcgg cagttctaac aagatctttc ttaagctgct
     6301 cggggatttt catttcgaag aatgaagtat aatctcccgc ataaatatca taggcatttc
     6361 ggtatgcgat aaatgcattg tttatatcac cacttccttc gtaagtcaag cccatcaaca
     6421 aatgcgcgaa tgcatcttct ttgtatttag gtttatcctt cagcttttct gccatggtaa
     6481 tcaacgaaag attcatgcga cggcattcaa ctaaggcatt ttcaaaatct cgctgtttga
     6541 ggtaattcat cgcattaaaa taatgaagca taaccacttc aaaatcttct gctttatagg
     6601 gtttaacgcc aggattaacg ataatggcga gcgcttcgta acccaagttc tttttgtaat
     6661 cttcaattag ataatctgct tcagcgaaat acttgctgct ctcggagtat ttcccttggg
     6721 cgaaggtgac atagcccatt tcgagcaaga ataaaagctt gagttttttc tttttcgctt
     6781 ttttagttga ggtaagtgtt gtttcagctt cagcgtattt ctcggtggca accagcgata
     6841 aaaaacgctc gtgccgcacg taatacgggg cacaagcgtt cattaaaaaa aggaaaaaga
     6901 ctatgctggt caggcgagtt aggcgcatca tcaactcgta attagagaaa aacaggaatc
     6961 ttaattaacg atgtattttt tccctttgta atcaccaatc caaacaattt cgtttgtttc
     7021 aatattggtt aattctaaat caatttgcca gtatgttgtt ttctctttcc cgtatccatc
     7081 aacaatagat ttaacggttc cttgcatcat aaaatcagca ccaacttctt tcccaaattt
     7141 tttcatggtt tcggctgaag agaaatcttg ctgatcagca cgttcgccgc ggatttcagt
     7201 tcttttatct cctgcagcta ccaagcgaat catcgtgctt ttgataaatg ctttttcgat
     7261 gtctttagtg aaaatttcgg tatccatgtg ctcgtggctg ttgttgcgaa caaatccaac
     7321 aataactact ggtttctttt tcttttcgtt ggtaaagttg cccaaccaat cgccagttaa
     7381 agcctgattc gttaatgttt ctgcagctaa acgagcgtct gtctcgttcc agcgaccgct
     7441 taagtcgatt actttatctg gtgcaactcg agttactgtg cggcgcgcac aagagctgaa
     7501 tccgattgtg ctaattaatg cgaggcctat caggcttttg atggaagttc tcataagaag
     7561 aggaattgag cttatttagt gtaggttgct tttactgttt gtgcaccgga gccatcatca
     7621 gatgtataat taagaatgaa tccagttcca ctataggttc ccgaaccagc gatgctaata
     7681 cctgtaattg attgctggct gatatcgaga gtattatttg caaccaaggc ttgaacgcgt
     7741 tgtgcagcac caaggttgaa aatgttgtaa atgagaatcc gagaggtgtt tgttgaatct
     7801 ttagttacag ttgaaggata atttccagta accgaaccgc caatttcttc tttcacattc
     7861 cacgttccaa tgaatttatc tttaggatca acttctggaa caattggctc ttcgtcggca
     7921 catgaggcaa ggaaaagagc aagaaaaacg aacgaaggag ctagtcgaga aagcgtcata
     7981 tataaaaagg ggattagaat gaatcgaatg atttattctt gaaactcgca taacaaggaa
     8041 agtgccaaca ataatttctt cgttgattta gctccagctt tgatcaatca aattacttag
     8101 ctcttcagct attttccggt cattgcttaa tctcggaact ttattttgac cgcctaattt
     8161 accttgagat ttcatgtatt taacaaaact gtctttctca agacttctca aaacaagtgg
     8221 cttcaaaacg ttgcctgtaa ttaggtcttt ataatagata ttctttttct gaagcgcagt
     8281 atcaatcttc attcgaaagc gctccggatc tttaggtttt acgctaaatt ctacaaacca
     8341 ttcatgataa ggcaatcctg tttcaggact aacttgtgga gctacagtaa attcaaccac
     8401 ttcaaccact tcgcccaggg atgcttcttt caaagcatat tcaacttctt cggcaataac
     8461 gtgttcacca aaggctgagg taaaatgctt aattcttcct gttactacca atcgataagg
     8521 gttggtacta acgaatttaa ctgtgtcgcc aatgttatat ccccacatac cggcattggt
     8581 attcaatatc agcacataat tcacatccac tttgacttct tttaacgaga tgcgagtagg
     8641 gttttcactg aaaaactcat caaccgggat aaattcatag aaaatacctg aatcaagaat
     8701 gagtaataat ccttgagatg gttgtttgtc ttgataggcg ataaatcctt ctgatgctgg
     8761 aaaaagctca actgaatcaa tcgatttccc aatcattttc tcaaaacgag aacgataggg
     8821 ttcgaaattc acgccgccgt atacaaacaa ggaaaaatta gggaacaaat ccttcatggt
     8881 tttgcctcca gatttctctt gtaaaatctc aaaatacatc tgcacccaag aaggaattcc
     8941 gcttattagc gtcatgtctt cagagattgt ttcttctgca attttacgaa ccttcgtttc
     9001 ccaatcttcg atacaattag tttcgaaaga aggcatttga tttcgtcgca aatactgagg
     9061 aacatggtgg ttgacaattc cactgagtcg tccaagcgga attccactaa tctcactcaa
     9121 cacgggactt cccgaaagga aaatcaattt cccgtctaca aattttgcat tgccagtttc
     9181 gtggatgtac agcaataatg cgtttcgagc cgattcgatg tggttagaaa tagaatcgct
     9241 ggtgatggga atgtatttag tgccggatgt ggtgccggaa gttttgcaga aataaatagg
     9301 ttttcctttc cataaaacgt ttgcttcacc tttggttgca cggtcgatgt aggtttttag
     9361 tccttcgtaa tctcgaactg gaaccttagt tttgaaatca tcataattac ggatcgtggc
     9421 aaaatcatgg tcttttccaa aagcagtatc ccgagcgcca gctataagtt gttgaaacac
     9481 tttttcctgt gtcacaatag gattattgct ccaattgcgc acttggcgtg ctattattga
     9541 tgctagcggt ttggcgaaga atgatttaag tcccataact ggagcaaaaa taggaagaac
     9601 tcagtcgata atttcttcta gaaagcatta ataaatacag gagagaatta atctgtacgc
     9661 gatgtttcat tttcgaaaaa ttcgcccaca taaatcaaaa tgcttcgtaa tgttgccgcg
     9721 gattaattac gatgaaaccc aaactcgaca tcattcaatt ttttcgggcg gtagcagctt
     9781 tgctagtgtg tttctttcac atgaaaggca tcctaaaaca aggtgaatgg gggaagttct
     9841 tgtttggaaa tggttctgtt ggagttccta tgttctttat gataagtggt tttatcatgt
     9901 atcacacaac tcgcatggtt cagccaaact ggaagaacgt aaaagcgttt ttgttgaaac
     9961 gactcatccg gattctacct ctttactatt taatgacgct actttatgtg ctattgttgg
    10021 ggaatgcgca ttattatttt attgaaaatc cgagcttttt attgccaact tttttctttt
    10081 ttccaaccta tgtgaatcta atcggtcctt cgtatggtat gcccccatta gcagttggat
    10141 ggtcgcttaa ctatgaaatc tacttttatc tgcttgttgg aatctccatg cttttcgtca
    10201 aaaaccgatg gtttatgtta tgtggatggc ttctcggtac tacgatattg attccacttt
    10261 tgagcaaggg atatgtaatg atgagtcttt cggaatgcta tggctatacc gcggtctact
    10321 tgaatttgat gaccaatcct gtattgttat tttttatagc aggtattgta attggcgccc
    10381 tttatactag ttcagtggtt ccgccatctt tgttttgggc cgatgcgtca gcacagttag
    10441 cagtcgtggt ttttattctt agttatttcc acctcataca attattacct ggttggtata
    10501 atcatttgtt aacgtgtggt tttctgctgg ttgctctttt acagcgaaat aaaatcaaac
    10561 cctattcgat tcctaaatca cttttaatca taggaaattg ttcgttttca ctttatctcg
    10621 ttcatccaat agtactcacc tatttgccta aggttttacg cactttaggt gttcaggctg
    10681 ttttagaagg atgggtgtat tttattggtg tactgctagt tatcgtagct atttcgtatt
    10741 taagctatgt gtggatagaa caacgattga gtctgaaaat tcaatcgttg gtgctgaagg
    10801 agtgatggtg cggattgaat ggattaatga atgaggctat ttatttcagc ttttaatttg
    10861 ggtaagtgat taatcaaaat cccccaaata gtttcatctg aaatagaatc ataagcatga
    10921 attatctgat ttctcaaacc aacgatgttc tttgcattat taattgggaa atgaggatca
    10981 acggcaagaa tccgcttaat cgcttctcca attatttcta aattcctttc gatagccttt
    11041 ttttaagaga atgtttttct gatatgcttc aaagcgcttt tcattatcaa tgaaaaagga
    11101 atcaatctca tcgatagcca gctttacatc gtataaatac ttaagtatct tttcgttcat
    11161 aaacgagctg cttttctcta tccacaactt tcctgaatac agggtttttg atggattgat
    11221 tttcaacgag atctactgat cggttgaata gacgttcgag ttcttcttta aaatcaaaat
    11281 agttatcagc atattgctct aattccacct tcccaaactg aaccaaaata tctacatcac
    11341 ttgagtctat aaatcggtcg gttaggattg atccaaatag atataactca tctactttgt
    11401 gtttttcaca aaggctatat agcttttctc tattggcatc tattaatatc atacacttca
    11461 aaaatacgat attaaatgtt ctgtagtcat caatctgaac acccttatcc taacaacccg
    11521 tccaaaatct cccttctcaa tacttccgac cacacatttg acacctgccc gaaacacacg
    11581 cacaatgcgc acataatccg tacataccgc tgctggtgcc ttttccacaa ttggaacaaa
    11641 cgcctatatc gtcggtaaca gaaacgagtc cgtgctctga gcaggtatag ctgcttcccc
    11701 aattctcatt gggacatttc ttttttttct catggatttt tttaccgttg atgtcgaaat
    11761 gtatttcatc atttatcggc tcgtcattgc ggtattcacg ttggcttttt agttggccgt
    11821 tagtatgcca agttttccat acaccatgtc gcaatccatt tttatatacc gattctgaaa
    11881 tcaatttacc ttgatcggta aagagtttcc acgagccatc gggcacacct gcaacactca
    11941 aatacacatc atcaatttct tttaagtagg ttggatttgg aacatactta gctgaaaaca
    12001 tcacttcggc aagcaatatt ccattcgagt tccattgctg aaaaagccct acaacttgat
    12061 ctttctcaaa ctgacaacga tgagatagtt gaccattagg atggtaatag gttgaaggtc
    12121 cctctcgcgt tataccatct cttttattag gatcgctgaa ttgatagtta agcgtaaaat
    12181 tcaatgcacc attttcataa taacctgttg agtgcattaa gcccggaatt agcgaatctt
    12241 ctctaacgtc agaacgcagt tttttacctt ctgtatcata gtagcgacgg caattactgc
    12301 gcaattctcc attgatgata atggcttcca acttacctct ttccatttgt tggtattctc
    12361 cattaataaa tccattccca ttacttgccg tttgattccc gttctcatcc caagcatcga
    12421 taatgatagg tattgaattt ctcaggccca tccgagcttt catcttacct gtttctggat
    12481 aatacatgag caactcatcg taattatttg cccaatgatt actgataaaa tgcactttac
    12541 aaagcaatcc ggattcgtta taatgtgccg accagccttt ggtcattcca tgctcgaatt
    12601 caatctccca tttgggcttc ccgttttcaa accaggcctt ccatgtgccg tgaggatatg
    12661 cattcgtgtc cacttcacca cttgatttca cttgtccgtt ttcatagaaa agcgagattt
    12721 ttccacaggg agttccattg caatactctc gttctagtgc gactttaccg ttgaggtgat
    12781 atttacgcca gatgcctatt cgtttgaaat cctgcatttg tcctgttgcc caaatcgcac
    12841 tgtcgttgag atgatttcct tgggcatcca tgaattgaaa ttcattagac gtgttccatc
    12901 cgcggtacaa acctgtccgg aactgccttg tgccgtaagg gaaattgaaa cgtgtgcgac
    12961 ttccttctgc ccagtaagtt ccattaaacg aatcttctct gtcgtagaaa tcgttttccg
    13021 gacgaacgcg tatcacggaa tcctcaccta tttttaacag ccagagtttc ttttgtgctt
    13081 gaccgaaatg attcgtgagt aaagcaagga agaggatgat aggtttcatt taaaagtttt
    13141 ggggagcatt caatacgaaa tcattttcca agatttcccg tcttcatttc gccagttttc
    13201 aatgtaactt tctagcgatg ccgccagaaa ataggggagg tttaataacg agtacacctt
    13261 tccggatgtt gtttttactt catctattga tgcttttcct tggtgaagac ggatggctaa
    13321 tgtggatggt gtttgatcca tgaaagcgtg taatgaacgt agttttccga tgcttcctga
    13381 ttttatttca acgggaataa tcaaggagcc acgttgaatg attaaatcaa cttcggcgct
    13441 cgattgcgat ttctcccgca cccaaaaaaa aggggatgaa tctgtatagt cttgaatggc
    13501 aatgcgttct tgaaacacac aatgtggaat aagagctcct ctggctgcat cacttaagtc
    13561 ttccaatcca attaattcag caagtattcc aagacgattc accaataatc cacagtctaa
    13621 aaactgtatt cgtggacttt tcctgtaatc aggctggaga ggcgttttta cgtcagtggt
    13681 ggggtaaatg agtctcaata gtctcgtgtc gtgtaattgc cgaaatgctt ctcctatttc
    13741 tcgtgtacga taagtagatt gtccaaaatt ttgaaaggtt atacgttgat cgaagcttgc
    13801 aggagcatta tttagaacaa accgaatcat ttgttttgct gacttatttc ctccgtattt
    13861 ttctacatca ttctgatagg atttccagat gctttcataa atctgttgga tgttgaatac
    13921 actgccatct tcgcgatacc gcgccaacac ttcgggcatg ccacccaaaa gacaatagag
    13981 attaaactgt ttatgaagct cctccaccgc atatgccggc acaggtagtt gcttcattgc
    14041 atcaatggct tgttgcttgt ccatcgcaat gagaaattcc tcgaacgata aaggatgtaa
    14101 atactggaac tcaacgcgtc caacaggaaa actgcgcaca cttcctaaag caaattccaa
    14161 aagtgaacct gctgcaataa cggagagttc agggaactgt tcatagaaat accgcagcag
    14221 ctgaatggca tcaggcactt cttgaatttc atctataaac agaagcgtat tctcccaatc
    14281 gggctttatc gattctctaa tcgtgagccg ttgcatgagt gccaccacat ccatatcgtc
    14341 cgagaaaagc tttttgtcgg cagcgagttc taggttaagt gaaacgaact gcgaatagct
    14401 ttttgagaag tctcgaacga gggttgtttt gcccacttgg cgagcgcctc gaagcaccaa
    14461 tggttttcga cctttcttat tccgccagtg ttccagtaaa gtaagccctt tgcgacggat
    14521 gtgtaacatt ttatttgtaa gataacaagg gcaaatatag cattatattg taagataaca
    14581 agggtaaata ttgccaagtt gtgtaagata acaagggcaa cttaagtgct tgctcctact
    14641 tcgcccaaat ctcgtcaatg aaaatgaacg cttcaccgcc tgcaccttga tgccattcgg
    14701 gaagtatgcc aaaattcttc gcttttacac gtatgtaacg catggttgtt ggctgaggta
    14761 ttgtcatggg ctcgttatgc tcattcgcaa caaacgaacg ttcttctacg aatttctgga
    14821 tttgtacctt agtgtctttg ggatcaataa aattgctgtc ggtaacaaat ggagtgaatg
    14881 aaattccatc caaggaatac tcaaatcgta cttcggtcgg gaataaaatc cagctgcgtg
    14941 aatcttgtaa aaaagaagcg ccaatcgtgg atatttcagt agttttttgc aaatcaacta
    15001 cgcattcaaa atcttgccct tgatagcctt gccatccacc tttgcgccag ttttcatcgg
    15061 caaataatcc atcaataata ccttggtcgc cgccggcaga atattgagga ttgtaagtgg
    15121 aactgatttt cacggtccag ttatgcggaa tcttgttgta tgctgcagta tttactgggc
    15181 tgcttattcc atctttaata acaatggctt ttaattgaca gctattctga attattattg
    15241 gatcggtagc tagtggagaa agagggctag gatcttctcc attcagggta taatagattt
    15301 tagctccggg tgcgttcgac tctattttca ctaaaacact atctcgaaaa acttgttgag
    15361 aagtagagaa tacgggagct tgaacagttt ttatagccga ccaatcgtat gtaaaaggta
    15421 tttgctgcaa ggtttgtttt tggggagcaa ctttacccat gctgagcgaa atagattcac
    15481 cattaatgat gctactgtgg ttgaaccagt agttttgttt tccttcaaca gcagtggtgt
    15541 attcgttttt acttgctcca ggagccttga tactaaagag cttgccgttg actggagtga
    15601 ttttgatttc atcgaaaatt ggagctccga ctacataatt tgggttaccc ggacaaacag
    15661 gataaaagcc cattgcagca aacacatacc aagcgctcat ctgaccacag tcttcgttac
    15721 ccgataagcc atccggagca ttgtgataca tctcttttaa gattcgtctc acatactgct
    15781 gagttaaacg aggttctcca attgcactaa acatccaagc catgtggtga ctcggttcat
    15841 tgccatgtgc atattgacca atcaaaccgg ttatatcact ttgcgtgcga ccggtggttt
    15901 ttgaatcggc ttcgaataaa cccgttaatc gtttaataag aatgtcttgt ccgccgtgag
    15961 cttgagctaa tccataaata tcctgaggaa cgaagaagct atattgccag gaattggctt
    16021 cggtaaagtg gttgtttact tcccgcggat caaaaggaga aagccaagca ccgttctttt
    16081 tcggacgcat aaatcctgat ttggattcaa ataaattctt ccaattttta gaacgagcag
    16141 cataggttga atagacagca ggtttcttca actcttctgc aaaacgtgct atacaccaat
    16201 cgtcgtaggc atattcgagt gttttagaaa cgctttcgtg ctcatcatcc accgataaaa
    16261 agccatgatc catgtaagct ggtaaaccca gatgattcca ggttgcactt ttctgcatgg
    16321 cttctaatgc gagttgtgta tcgaaacctt taatgccttt gaaatacgcg tccaccatta
    16381 cacttacgct gtgatagcca atcatgcaat cggtttcgtt ggaggcaagt tcccaaacag
    16441 gtaatcttcc accttcttgg tacatgcgca aaaaggtttg aatgaaatcg cgtgttcgtt
    16501 ctgggtaaag aatcgcatat aatggatgag cagctcgaaa cgtgtcccaa agcgagaata
    16561 ctgagtaata agtaaatcct tcagctttgt gcaccttctt atccataccg cgataagaac
    16621 catctacatc gcttactgtg ttgggttgaa tcatggtgtg ataaagtgcg gtgtaaaaga
    16681 tcttttgttg ttcggtggtt ccgcctttga tttgaatccg attcaactct gtttcccagt
    16741 attttatggc tctttcttta gctaaatcga agttgacatt cgttgcttca ctggttaagt
    16801 tgagatgagc accttcgaaa gagctaaacg aaacgcccac ttttacttcg agcacattgc
    16861 tactcatgtt ttcgaattga atagctgttc gtaaatcgcc ttgtgtgcta tttgaacttt
    16921 cttcgataac agaaattttt tgagagaaag cgatttcgaa ataaattacc tgatcttgag
    16981 cccaagcctt actgcgacgc attccacgaa tggtatggtc gtctactttt tcgactttac
    17041 cttccaataa ttcatctcgg tgaagcaagt ctaataacac aaaaccattc ttccccggaa
    17101 aagtataacg atgaaaaccc acacgagtac tggttgttag ttcagcacga atttgttggt
    17161 cacgtaaaac cacctcatac caacccgctc cagcttgttc ggctttgtga gaaaaactgc
    17221 tggcaaaatg cttcttggtc aaatcgggac gattcatcaa gggcatgagt aaaatatcac
    17281 cgtaatcgca gcaacccgta ccgcttaggt gagtatgtga aaatccataa atcaaggaat
    17341 cgctgtaatg atagccgcca cagccatccc agcttccatc aacacgtgtg tcggggctaa
    17401 gctgtaccat tccaaagggc accgttgcac cgggaaaggt atgtccatgt cctcccgttc
    17461 caaccatagg attgacgagt ttaacaggag tatattgagc aaaagccgca gaaacgaaaa
    17521 gcgacaggta aagaaatatc agttggcgca tattgaaaaa tggagccaaa aggtagggat
    17581 tattgaaacg aggctgaact cagttcaatc agcaattaat caacgttaaa acagttaagc
    17641 ctcagaactt aatgcgcgaa ttggagtgca attcaccatc actatacatc tgaagagcaa
    17701 tttcggttcc ttcaatttgt ccgttaccag tagttaagaa gccattaata acaagaggta
    17761 ccatagtaac tgtattatta tcacagttga agttaagtgt tacatcgaac gtggttaagc
    17821 ctgaaaccac taattgacca gatgaattat aggtacatgt gatgttctta ttcaacaaac
    17881 taccattgct gatataggtg atttgtccaa ttgtgtagtt tctaaatgca tatccacagt
    17941 ttggacgata cacgaaatac accgttttag ttttaggagt actgttttca ttaaagattg
    18001 aaacggaaag atcagcagga taagcgcctg gtgttgcaaa ggtctggttg aatgaaacat
    18061 tgaagttccg gctttcattg gaaggaatgg tgagctgaga accaacatca aatccacctg
    18121 ttactgtttc aaaactagct cctaattcct ttgaagaagc acattgaaca gcaacagaat
    18181 agggcaaagt accactatgt tcaaaaatca aggaatcatt tgaagtgaat gaaaattcag
    18241 tttgttgcga aattggctct tcatcttctt tacaggatga aattgaggca atagccaata
    18301 aaagaataaa atagatgcgc gtgtatttca tttagcaaaa gtaacagagg ttgcgtttat
    18361 ttctttaatg tgatttcttt tttcgtgtgg cattgaagca gtaagtgata gtaggacgaa
    18421 atagaatata gttagttgac aagaaatcat tattcaattc agttaaaaag cctgcagtga
    18481 atgctaacaa gaatcttcga tgagtattgt gagtgtaatt aaaataaact cccaatcgtg
    18541 gaatatgttg gctttgtaat gtcgtcaagt agttgaaacg aaaactatag cccaatttac
    18601 tggaagcatt aacaaaagct gctgaatagg agaagtcgaa accattgtac aaatgtgctt
    18661 tatgccaatg cctccaataa tagtttgtgc catcaacttc atattgcttt tcatcgcctc
    18721 gaacatagcg taatgtaact tgatggttgt tgcccttttg ggttaaagta tagtatccac
    18781 ctcctgctgt aaatgacctg aaatcactgc cataaccata tcctgttgga aaaataaagc
    18841 ctcctgttct aacgccagat gtcacaaacc aacgggaaga tacggcataa cttacattta
    18901 actctccacc aattggccta ttagtaagat tcaaattaat gcccgttgta attcgaacat
    18961 cctttttttc ttctaataat tcaggttgat ttccattaaa gtctgcaatg tgattgattg
    19021 agcaggaact catcaataag attgaccaaa tgcaaactaa taagtactta taatgtagca
    19081 tttagaaata gtggattaag tatagcttta tcaa
//
LOCUS       DAOOQW010000028        18825 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_3571_length_18825_cov_6.520618, whole genome shotgun sequence.
ACCESSION   DAOOQW010000028 DAOOQW010000000
VERSION     DAOOQW010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 18825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18825
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3571_length_18825_cov_6.520618"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1493)
                     /locus_tag="PK281_03215"
     CDS             complement(<1..1493)
                     /locus_tag="PK281_03215"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOY48382.1"
                     /translation="MKKQVYLSFLFLLFLLPTVALGQKTGSITGTVIDKATGETLPGA
                     SISIEGTTTGTMTDIEGRFKLANLAAGSYKIVCSMVSFSKKILSGVEVKEGAATNLMI
                     SLETVQNDLKEVIIEAEAKRESINALVLQQKNAASVSDGISAETIKKTPDRTTSDVLR
                     RISGASIQDNKFAIIRGLNDRYNASYINGAPLPSSESDRKAFSFDIFPANMLDNIVIN
                     KTATPDMPAEFAGGIIQINTKSIPEENFQSFSISSGYNTMTTGKEQLTYKGGKTDWLG
                     LDDGTRALPSAIPSKQDFPTLPAGQADLGKNFTNNWGINSSSFAPNFNAQYSLGHTIR
                     RKEKDLVGLVFAASYQRTYNYLITERKGFTNSTDPSVPSQLDFSYTDNSYATQTLAGL
                     LGNVSFKINKNNTISTKNLLSINADDRVIRREGTPTPLESNPLLLRSTAQWFTSNRIY
                     TGQLIGEHLIGSTKLKVNWVGSYSSIQRSIPNLRRNVYTRLDHSNDPG"
     gene            complement(1828..2466)
                     /locus_tag="PK281_03220"
     CDS             complement(1828..2466)
                     /locus_tag="PK281_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48383.1"
                     /translation="MRKIVLFFILFLSINSIHAQIKFSDFIDEFTVSINQALSSNKKQ
                     KNTGFGLGVYHTISPSSYLNILVGFEYNRVNQFYNTNYSSHFDSKSNEYFRLNFISFP
                     IQLRIKGSLGSHNEMFFSPGVYLDLAQKSRAEYDYHSVVVKNNVPTYYSGHLYENAGS
                     NQVGISLGTGMKFKKLKHDYLIHLDLKIPVMKLINEVFASYLRLSLGMSLKK"
     gene            complement(2499..4331)
                     /locus_tag="PK281_03225"
     CDS             complement(2499..4331)
                     /locus_tag="PK281_03225"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOY48384.1"
                     /translation="MKNLNFTFIFLCLLPSFCIAQKQGQDRIDSLILVLPSMSEDSNK
                     VKMLEEISFSSYSINPQQGLKYGFESLNLAKLIKWDKGIARAYNTIGINYGFGRADYP
                     NALDNYLKSLTINERLGDQKEIAASLNNIGSLYDETGENQRALDYYNRALVINQKMGN
                     KDWMANNLSNIGVLYTKLKEFNKAIQYKKEAMKLKEELGDQWGIATNTSQLARIYGEM
                     GQLDTAIILFHKALDIFTKLGDQNSIAKNLDHLGTAHLYMAANKPQLRRELLDKALLY
                     NDSALTIYMSIRHLQNISGSYISRSEIMHALGKDNEALEDYKVAMLYKDSVFNMEKDK
                     KLVQQAMQYDFDRKEAINLAEQEKKDIRQKLIRYSMATGLSGAFIFSLIVWRQRNRIS
                     KEKARSEELLLNILPGEVAEELKIKGEAEAKLIDEVTVLFTDFKGFTALSEQVTPKEL
                     VNDLHECFSAFDAICEKHGIEKIKTIGDAYMAAGGLPTPNSTHAKDVILAALEMAQFI
                     EEGKAQKIAAGLPYFEIRIGVHTGPVVAGIVGVKKFQYDIWGDTVNTASRMESSGEVG
                     MVNISETTYQILKDDSTFVFEPRGKVQAKGKGEIEMYYVKKAKV"
     gene            complement(4383..4802)
                     /locus_tag="PK281_03230"
     CDS             complement(4383..4802)
                     /locus_tag="PK281_03230"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOY48385.1"
                     /translation="MGLKIKTIGDAYMAAGGLPTPNSTHAKDVILAALEMAQFIEEGK
                     AQKIAAGLPFFEIRIGVHSGPVVAGIVGVKKFQYDIWGDTVNTASRMESSGAVGKVNI
                     SEATYSLVKDSVTCTFRGQVEAKGKGNLGMWFVEEIV"
     gene            complement(4774..6393)
                     /locus_tag="PK281_03235"
     CDS             complement(4774..6393)
                     /locus_tag="PK281_03235"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOY48386.1"
                     /translation="MMEGYRLTNGFRIDIQLCIRFILVFIFQIGMLAAQTNPQEALNH
                     LKKEVGIATHDTTRASIMLKIAQQLVSLGQLEEVVVYADSGLIYARRAEALKIQSELL
                     RTKGQSFYYRGRIEEAQKTWNEGMELAKELNDPDQINLFRGLEANIYMARSDHSRALE
                     IYLDLLKDAEAKKKNKQIQVYSANIGGIYIRQNKAKLAIPYLERSAEICKTMFDPSAS
                     AKTLITLGTAYTLTKQYAQAELTLKEAIKVGEQVNSPALLAPAIQGMVQVYQAKGELD
                     LALRELEKTEQLFKSVGDQCQVGMAMGQRSDFLFQLDADTNKIILQRLFNGNHDQALK
                     SALLVADSATVILEKCGDIYNLSLTLNVRSKIHARLGNYKSAFDDHVRFKTLNDTLFN
                     QERDKQITENSMKYEFEKKEAATLAEQKKKDQRQVLIRNGIIAGLLITLFFLLIVWRQ
                     RNRISHEKQRSEDLLLNILPEQTAEELKLTGTAQARQFDLVTVLFTDFKGFTALSEQV
                     TPKELVNDLHECFSAFDLICEKYGVENQNNR"
     gene            complement(6631..7215)
                     /locus_tag="PK281_03240"
     CDS             complement(6631..7215)
                     /locus_tag="PK281_03240"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HOY48387.1"
                     /translation="MIALENETLQNLISDLNLTNDTLSDHLNHLAINESKHLRDLRIN
                     LKNALSFSNLTRKESLLIAFSVAVNDRTKNLADSLESLARAEGATESELAEVRACVSL
                     LNVNNVFYRFRHFTKKDYYQQTPAGIKMGIMINPILGKEFFELLSLAVSALNGCELCV
                     NAHENSLLQLGTSEARVYDAVRCAAIFRGMTVLS"
     gene            complement(7227..7796)
                     /locus_tag="PK281_03245"
     CDS             complement(7227..7796)
                     /locus_tag="PK281_03245"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013597301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HOY48388.1"
                     /translation="MNRIISLGESFPTFEKTSVTSLEKGKEFSTLSLDQQLGTGKWMV
                     MFWWPKDFTFVCPTEIAEFNKNYSEFSDRDTNLIGASTDSEFVHLAWRQNHPDLKGLR
                     FPMLADTSKSLAEELGILEANEKVAYRATFIVDPQGIIRWVSVYDLNVGRNVEEVLRV
                     LDALQTDELCPCNWKRGEETLSISDVVEA"
     gene            7901..8836
                     /locus_tag="PK281_03250"
     CDS             7901..8836
                     /locus_tag="PK281_03250"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HOY48389.1"
                     /translation="MTLTQLEYLIAVDTHRHFARAADACFVTQPTLSIQLKKLEDELG
                     VALFDRGKQPVVPTDAAIRILDEARQMVQLGKRIKEQVKDETEELQGLIRIGFIPTVA
                     PYLLPLFLGEFQAKYPRVELEIEEITTENLIAKLKRDELDAGIAATPLPESNLHELPL
                     YYEPFVAYVAMKDPLLKKSAIRISDINLSNLWLLAEGHCFRSQVLNLCQMQDKSGSDI
                     HIHLKAGSIETLKGLVEHNAGVTLLPEFSTRGFNDEQRDQLRYFKTPEPVREISLITC
                     RQVVKQRLTEALCKTILSVIPEKFKSPVKSNIIPI"
     gene            8883..9545
                     /locus_tag="PK281_03255"
     CDS             8883..9545
                     /locus_tag="PK281_03255"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HOY48390.1"
                     /translation="MKRTLSILIPLLAALLFGIYYFSNKEKTRFYKRLPILGNHSIDS
                     TRSGSTVKYDTLWHRIPNFSFTNQDGQPVTQTDVQNKVYVADYFFTTCQSICPIMSKQ
                     LDRVAAAYKNEADFRILSHTVDPETDTPAQLKGYAQSYKANTKQWIFLTGEKPALYEL
                     ARKGYLLNLEEGDGGPDDFIHTQNFALIDRHQRIRGFYDGTDSAQVNNLIQDIGFVLQ
                     EK"
     gene            complement(9882..10838)
                     /locus_tag="PK281_03260"
     CDS             complement(9882..10838)
                     /locus_tag="PK281_03260"
                     /inference="COORDINATES: protein motif:HMM:NF022720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HOY48391.1"
                     /translation="MKRIFNLIAFLVFSSTLLQAQIEVAEDTSVNGWKKGGFFALNFN
                     QVSLTNWAAGGESALSSTAMVNAFVKYRHNDLYFESVLDGGFGFITTKSQSLRKNEDK
                     IEVNAKFGKKAKGKFFYAGMLNFRTQMAPGFNFPDDSTVISRFLAPGFLSLALGMDYK
                     PNDWFSMFISPATGRATFVMDQRLADLGQFGVTAAVIDPLTGAVITPGEKTRFEFGAY
                     LRARLQKDLVKNISVVSNLQLFNNYTDPIAAQRSNIDVNWENLILIKANKWLTTSIFT
                     NLIYDNDIQVPVKRMVNGVEVEGKGPRTQFKEVLGVGLSFKF"
     gene            complement(10850..11233)
                     /gene="mscL"
                     /locus_tag="PK281_03265"
     CDS             complement(10850..11233)
                     /gene="mscL"
                     /locus_tag="PK281_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002681172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HOY48392.1"
                     /translation="MGILKEFKAFAMRGNVIDLAVGVVIGGAFGGIVNSLVGDVIMPI
                     IGVLTGGVDFSGLSYTVGKAVIAYGKFIQAIFTFIIIAFSLFMVIKGMNATKKKEEEA
                     PAAPPAPSKEEVLLGEIRDLLKDRR"
     gene            complement(11402..15625)
                     /locus_tag="PK281_03270"
     CDS             complement(11402..15625)
                     /locus_tag="PK281_03270"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY48393.1"
                     /translation="MNRLFTSILPVILALVAGTASAAEKIWTGSSGSNWSTPANWQPA
                     GTPTQSDDVVFDGTVSSISCTLPSNVIVRNLSVLPSYTGTITGVNQSSAILTVLQELH
                     LSGGTISTGLSKLKVAGRLWVDGGTLTKGAGGICSLLNVKIDGGSVTIGNSTVEVLGA
                     MEITGGSFQTGSGTFMVSVSYSQSGGTFTKTAGVGTFNPSGALSITGGTFNCQSAAIS
                     FKALQLSNAILNGGTSSIYMNGNSSIDNATFNKTSGAVYMNNNASFTANNSSIDFGSS
                     TFNAHALTIQGGIVNLGTGSLVVDGVCSFNGATITKASGNSNFALAQTLTISGGSVSF
                     GTGLVNTGALHLSNGDLSFGSGVLNIAGALTVDGTSSLSKNAGVALLSIGSDVNFISG
                     TIDFTGSTQIQTGNWIQSSGIATTGSCSMLVNGNFELSNDAEFTAPTSLLDVKGNFRR
                     ISGTFNHAYGTVRMSGTSSIPYSILGNPFFNNLEFYNTSGVNNKTIEVYGTISVNGDL
                     LLTNASASNRAMRINTGTISIYGNLNIANYRCTEVNPGTATLRFTGGSTQTIFGTSLT
                     DGVGILPAIYVDKTGGVFQLFGNVNFGNGFSHQNSTIEFDPEMVFGMAGGNFFLEGLL
                     LPVIHVSGFAKLTSSVAVLGNLEILPNGFLMNGSDALNVNSELINNGRFQNMSGSLNV
                     SGVLTNNGEFAVNSGSMNALSGIIQELGVFTCGTGQVNTLGTLTINNGSFNCASGIVV
                     VSGSINQNGGILNGNTSGGSLVISQNFNQNSGIFHGQTGTMSIGSSLNMSGTFVRGTG
                     TVNFNGFGPQTVPALHYNKLSIAGSGRLITLAPGEIKIGAATGGFNPHVSNLYTTANN
                     TINYNNNGNQDITGFVYNNLTVSRIGTKSIIANTSVKNTLEVLNSAVLDADGINNNKV
                     MTLLSSQSLTARIERIPSTASISGNMTVQRWTRGGIRSNRFFGSPVDTIGGIKFKQVK
                     DNILLYGPGNGLNGWDNPTLFTTNVFVYNESLLNGYEWRSPASTSEVLPKGAGFLVYH
                     LGDRSQVNLQVNTIPNAAIIDFKGNPNQGDIPLPVTCTGGCLPVDNGNGWNLVANPYA
                     SPIDWDSPEWTKTGISSTIYIWNPRINQYASYNTSNPGAATNGGSRYIGPGQGFFMKV
                     TQDNPSLIAHESIKTSQFPDSLLFRIGAAQSQLRMLINKQDSEVQDEAVLEFRHEASE
                     DFDPALDAHKPELPGMTLSFGLMNEKGDALSVHALPKPDIAGSDKIIPIRLKGDVGSY
                     SIQASQLESFDSDVVFYLEDRLMKTIVKVEEGKSYPVEITNDPASSATGRLNLILRHN
                     RSNSLAGSEMRVFPNPSNGSNVQVVLPKAGRGTLEVYDMLGAKRVAKNIETTQQTIQL
                     DAIDNLAAGTYTLVWTSIEGRYSELLTVK"
     gene            complement(15898..16197)
                     /locus_tag="PK281_03275"
     CDS             complement(15898..16197)
                     /locus_tag="PK281_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009780019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HOY48394.1"
                     /translation="MLIRIVEMHFRLDAREEFLEVFHQSKEKIRAFDGCEHLELLNEP
                     NDPGHFFTYSYWKDEASLERYRQSELFQSTWSKTKILFDQKPRAWTLEQLVSLPQ"
     gene            complement(16178..16975)
                     /locus_tag="PK281_03280"
     CDS             complement(16178..16975)
                     /locus_tag="PK281_03280"
                     /inference="COORDINATES: protein motif:HMM:NF014003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HOY48395.1"
                     /translation="MAIITLTTDMGLSDYYVAAVKGAILTQLPNATIVDISHEIPKFD
                     RMKAGFILRNAYTHFPPGTVHIVGVETLEDEDTQHVALKINGHFFVGADSGIFSLLFS
                     PQQADEIVQLNIRKEQTPKSFPTVDLFVTAACHIARGGTLEIIGRKISGFRQSLMLSP
                     TRGESHLGASIIYIDSYGNLITNIRQEQFEERRQGGKFEVVLRRASQNIVRISKSYNE
                     VPEGDMCALFNASGYLEISINRGSAAKLIGLKVNDTLRVEFHVDTNR"
     gene            17079..18050
                     /locus_tag="PK281_03285"
     CDS             17079..18050
                     /locus_tag="PK281_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007651277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOY48396.1"
                     /translation="MIEKTIHLPDVNPVEIYGVNDETLDLIRAFYPGLRLVARGLDLK
                     IQGEEPEIAAFETKLNRLISHQVQYGSLSEQLVKKLMNPENDLPEMTPDQNGASDVIV
                     FGNNGLIVKARTPNQRRIVNASFTNDMVFAIGPAGTGKTYTAVALAVRALRNKEVKRI
                     ILTRPAVEAGENLGFLPGDLKEKLDPYLQPLYDALLDMIPADKLQLFLETGIIQIAPL
                     AFMRGRTLDNAFVILDEAQNTTEAQLKMFLTRMGRSAKFLITGDLTQIDLPRNQSSGL
                     ATGIRILKDIAGIEIIHLDTSDVIRHKLVTAILHAYQANQPDKPARD"
     gene            18007..>18825
                     /locus_tag="PK281_03290"
     CDS             18007..>18825
                     /locus_tag="PK281_03290"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008991806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HOY48397.1"
                     /translation="MPTKPTSQISLRATDFHFEGQTAFYRGKVRDVYTIKNQYLVMVA
                     SDRISAFDVVLPKGIPYKGQVLNQTATYFLEATRDIVPNWLLGTPDPNVAVGKMCTPF
                     KVEMVIRGYLAGHAAREYALGKRVICGVSLPDGLKENDRLPEPIITPTTKAAEGHDED
                     ISREEILRQGIVAEADYVLLEKYTQALFKRGSEMAAERGLILVDTKYEFGKTDSGEIL
                     LIDEIHTPDSSRYFYADGYAERQARGEVQKQLSKEFVRQWLIANGFQGKEGQQIP"
BASE COUNT         5778 a         3853 c         3306 g         5888 t
ORIGIN      
        1 cccggatcat ttgaatggtc caaacgtgta taaacattcc gacgtagatt aggaatagaa
       61 cgctgaatgg agctatacga gccaacccaa ttaactttta atttagttga acctattaaa
      121 tgttcgccaa ttaattgtcc ggtataaatt cgattacttg taaaccactg agcagttgaa
      181 cgaagtaata gtggattact ttcaagaggt gttggagttc cttcgcgacg aatcacacga
      241 tcatcggcgt taatactcaa taaattctta gtagaaatgg tgttgttttt attgattttg
      301 aaggatacgt ttcctaataa accagcaagc gtttgggttg cataactatt atcggtgtag
      361 gagaaatcga gttgagatgg cacacttgga tcagtgctat tagtaaagcc ttttcgttcg
      421 gtaattaaat aattgtatgt acgctggtat gaagcggcaa acactaagcc tacaagatct
      481 ttttccttgc gtcgaatagt gtgaccgagt gaatattgag cattaaaatt tggagcaaaa
      541 ctgctggaat taattcccca attattggtg aaatttttac ctaaatcggc ttgtccagct
      601 ggtagagttg gaaagtcctg tttagaagga atagcactag gtaaagcgcg agttccatca
      661 tccaatccta accaatcggt tttgcctcct ttataagtaa gttgctcctt tccggtagtc
      721 atggtattat atccagaact aatgctgaaa gattggaaat tctcttccgg aatactcttg
      781 gtatttatct gaataattcc accagcaaat tcagcaggca tatcgggagt tgccgttttg
      841 ttgatgacga tattatcgag catgttagct ggaaaaatat cgaatgaaaa agcttttcga
      901 tcgctttcag aactaggtag tggtgcacca ttaatatagg aagcattata tctatcattt
      961 aagcctcgta tgatcgcaaa tttattatct tggatacttg cgccacttat acgacgcaaa
     1021 acatcactgg tagtccgatc gggcgttttt ttaatggttt ctgcagatat accatccgaa
     1081 accgatgcgg catttttttg ctgaagaact aatgcattga tggattcacg cttagcttct
     1141 gcttcaatga ttacttcctt taaatcattt tgaaccgttt caagtgaaat catcaaattg
     1201 gttgctgcac cttcttttac ttctacccca ctcaaaatct tcttcgaaaa cgacaccatc
     1261 gaacatacaa tcttatagga gccagctgct agattagcaa gtttaaagcg cccttctatg
     1321 tcggtcatgg ttcctgttgt ggtgccttcg attgaaatac ttgcacctgg caatgtctct
     1381 ccagttgctt tatcgataac ggtaccagta atacttccag tcttttgccc caaagctaca
     1441 gttggtaata gaaagagaag gaaaaggaaa cttaggtaga cttgtttttt cacgagactt
     1501 gaattatcag aaagatgcat ctgtttcttt ctgcaaaatt gcctcggcta cttctctaaa
     1561 ctgtaaatca tacgttaagt gcgcgatatc aaagcggggt taaatattaa cagagaatta
     1621 ccgcaacctc aatttaggtt aagaattgag tacattgaaa ttcacactaa gttaacattg
     1681 gcgtaagagg ccagtaaatt agaggcattc ccaaatagtg aaagttgttg cctgaagtaa
     1741 atcatcaaac attttgacaa tttacattgt cgtgatttta atttaatatt ggagtatcac
     1801 taattgtagt gttaaggagt tttctatcta tttctttaaa gacatgccaa gagataagcg
     1861 taaataagat gcaaatactt catttattaa tttcatcact ggtattttca aatcaagatg
     1921 gattaaataa tcatgcttca attttttaaa tttcattcct gttcctaaag aaattccaac
     1981 ttgattacta cctgcattct catatagatg accagagtag tatgttggta cattattttt
     2041 aactacaacg gaatggtaat cgtattcagc tcgtgatttt tgagctaaat ctaaatatac
     2101 gcctgggcta aaaaacattt cgttatggga acctaaagac cctttaattc ttaactgaat
     2161 tggaaaggaa ataaaattta aacgaaaata ttcatttgat ttcgaatcaa aatgactcga
     2221 gtaatttgta ttgtaaaatt gattcacacg attatattca aatccaacta ggatatttaa
     2281 ataggatgaa ggagaaatag tgtggtaaac tccaagtccg aatcctgtat ttttttgttt
     2341 tttattgctc gataatgctt gatttattga cacagtaaat tcatcaataa aatcggagaa
     2401 ttttatttgt gcatgaattg aatttataga tagaaataaa atgaagaata gtactatttt
     2461 tctcattatt aaaaatttaa acttagtgat gaaccggttt acactttagc ttttttcaca
     2521 taatacattt caatctcccc tttacctttt gcttggactt taccgcgtgg ttcaaacaca
     2581 aatgtagaat catcctttag aatttgatag gttgtttcac ttatattgac cattcccact
     2641 tctccgctac tctccattct actggcagtg tttacagtat ctccccagat gtcatattgg
     2701 aattttttta ctcccactat ccccgctaca acaggtcccg tatgcacacc aatgcgtatt
     2761 tcgaaatagg gcaatccagc tgcaattttc tgagctttac cttcttcaat gaattgagcc
     2821 atttctagtg cagcaagtat cacatcttta gcatgagtag aattaggtgt tggcaaccca
     2881 cctgcagcca tataagcatc accaatcgtt ttgatttttt caattccatg tttctcgcat
     2941 attgcatcaa aagcactaaa acattcatgt aaatcattga caagttcttt aggtgtgact
     3001 tgttctgaaa gtgccgtgaa acctttaaag tcggtaaata atactgtcac ctcatcaatt
     3061 aatttagctt cagcttctcc cttaattttt aattcttcag ctacttcacc aggtaaaata
     3121 ttcagcaata attcttcact acgtgctttc tctttagaaa tacgattgcg ttgtcgccat
     3181 acaattaatg aaaatataaa cgctccagat aatccggtag ccattgaata tctgattaat
     3241 ttttgacgaa tatctttttt ttcctgttca gctagattta tagcttcctt gcgatcaaaa
     3301 tcgtattgca tagcttgttg aaccagcttt ttatcttttt ccatattaaa tacggaatcc
     3361 ttatataaca tggctacttt ataatcttct agcgcctcat tgtcttttcc tagtgcatgc
     3421 attatttcgc tacgcgatat gtaagaccct gaaatattct gcaagtgcct aattgacata
     3481 taaattgtaa gtgcactatc gttataaagc aatgctttat caagtaattc cctccttaat
     3541 tggggtttat tggcagccat atataaatga gctgttccta agtgatctag attctttgca
     3601 atgctatttt gatctccgag tttagtgaaa atatccaagg ctttatggaa aagaattatg
     3661 gctgtatcaa gctgccccat ttctccataa attctagcaa gttgactggt attcgttgct
     3721 atcccccatt ggtcaccaag ctcctctttg agtttcatgg cttccttttt atattggatt
     3781 gctttgttaa attcttttag tttagtgtat aatactccaa tgttactcag attattagcc
     3841 atccagtctt tattccccat tttttggtta atgactagag ctctattgta ataatccaat
     3901 gccctttgat tctctcccgt ttcatcatag agcgaaccaa tattatttaa agacgcagca
     3961 atttcttttt ggtctcctaa acgttcatta attgttaatg atttcaagta attatctaaa
     4021 gcattgggat agtcggcccg accaaatcca tagttaattc caatcgtgtt atacgctcga
     4081 gcaattcctt tgtcccattt gatcagcttt gctaaattga gcgattcgaa tccatatttt
     4141 aagccttgct gcggattaat tgaataacta ctgaagctta tttcttccaa cattttgact
     4201 ttgttggaat cctctgacat agatggcaaa acgagaataa gcgaatcgat acggtcttgc
     4261 ccttgttttt gtgcaataca gaacgaagga agtaaacaga gaaaaataaa tgtaaaattt
     4321 agatttttca taggttataa tctactaagg tgaatcaaat aaaatccaca atctaaccat
     4381 cattatacaa tctcttccac aaaccacatc cccaaattac ctttcccttt tgcttccact
     4441 tgacctctaa atgtacacgt gactgaatct ttcacaagag aataggtagc ttcactaata
     4501 tttacttttc caacggctcc actgctctcc attcggctag ccgtatttac agtatctccc
     4561 caaatatcat attggaattt tttcacaccc actattcctg ctacaacggg tccactatga
     4621 acgccaatac gaatttcgaa gaaaggtaat cctgctgcga ttttctgagc tttaccttct
     4681 tcaatgaatt gcgccatttc taatgcagca agtatcacat ctttagcatg tgttgaatta
     4741 ggtgttggta gtccacctgc tgccatatag gcatcaccta ttgttttgat tttcaacccc
     4801 atacttttca cagattagat caaaagcgct gaaacattca tgaagatcat taacaagctc
     4861 tttgggagtt acttgctccg aaagcgctgt gaaaccctta aaatcggtga ataaaacagt
     4921 caccaaatca aattgtcgag cttgtgctgt acctgttagt ttaagctctt cggctgtttg
     4981 ttctggaaga atatttaata acaaatcttc gctcctttgt ttttcatgac tgatgcgatt
     5041 tcgttgtcgc caaacaatta gcaggaaaaa taaagtaatt aataaaccag caattattcc
     5101 atttcgaatt agaacttgtc tttgatcttt cttcttttgt tcggcaaggg tggcagcttc
     5161 tttcttttca aattcatact tcatagaatt ctccgtaatt tgtttatcac gttcttgatt
     5221 aaataatgta tcatttagtg ttttaaagcg aacatgatcg tcaaatgccg atttgtaatt
     5281 tcccagtctc gcatgaattt tacttcgaac attcaatgta agagataaat tatagatatc
     5341 accacatttt tccaaaatga ctgtggcaga atcagcaacc aataatgctg atttcaatgc
     5401 ttgatcatga ttaccattaa ataatcgttg cagaattatt ttattcgtat cagcatcgag
     5461 ttgaaataaa aaatcggaac gttgacccat tgccattcct acctgacatt gatcgcccac
     5521 acttttaaat aattgctccg ttttttctaa ttcccgcaaa gccaaatcga gctccccttt
     5581 tgcttggtaa acctgcacca ttccttgaat agctggagct aacaatgctg gagaatttac
     5641 ctgttcgcca acttttattg cttcttttaa tgtcaattct gcttgcgcat attgtttcgt
     5701 caatgtatat gctgttccaa gtgtaataag tgttttagca cttgcgcttg gatcaaacat
     5761 cgtcttacaa atttccgctg agcgttctaa ataaggtatg gctaatttgg ccttattctg
     5821 acgaatatat attcccccaa tattggctga ataaacctga atttgtttgt ttttcttttt
     5881 agcttctgcg tcttttaata aatctagata tatttctaat gcccgtgaat gatcacttct
     5941 agccatgtaa atattggcct ctaaacctct aaataaatta atttgatctg gatcatttaa
     6001 ttcttttgca agctccattc cttcgttcca agttttctga gcttcttcta ttctaccccg
     6061 ataataaaac gactgacctt tagttcgtaa taattccgat tgtattttga gtgcttctgc
     6121 ccgcctagca tagattaaac ctgaatcggc ataaacaact acctcttcta attgtcctag
     6181 tgaaactaat tgctgggcaa ttttcagcat aatagaagca cgagtagtat catgcgtagc
     6241 tataccaact tctttcttta aatgattaag ggcttcctgc ggatttgttt gtgccgctag
     6301 cattccaatt tggaaaataa atactaaaat aaatcgaata catagttgaa tatctattcg
     6361 aaaaccattc gttaatctat agccttccat cataaaagat tggattaaaa aacaaattaa
     6421 ttaactatca attccaaatc tccaatcaag caattcatga taaaaggcga aaaaaaaatc
     6481 tttctccctc ttttacatgt aattcagctt attcaccact acctaatcta actaatgcct
     6541 aaatttctgt ctaaatatac tgatttggaa aatagaaaaa ccggcattct cccgaaagaa
     6601 aataccggtg gttgagaaaa aatagatggt ttaacttaat actgtcattc ctctaaaaat
     6661 tgctgcacat ctcactgcat catacacacg tgcttcgctt gtgcctaatt gaagtagcga
     6721 gttctcgtgt gcattcacac ataattcaca accattcaaa gctgaaacag ccaaacttaa
     6781 taactcaaaa aactcctttc ctaaaatcgg attgatcatt atacccattt taattccggc
     6841 aggtgtttgt tgataatagt ccttcttggt aaaatgacga aaacgataaa acacattatt
     6901 cacatttaac aaagaaacac aagctcgaac ttcagctaat tctgattcag tggctccttc
     6961 agctctagca agactttcaa gcgaatcagc taaatttttg gtgcgatcat ttactgcgac
     7021 tgaaaaagca attaataaac tttcctttcg tgttaaattc gaaaaagaaa gtgcgttttt
     7081 caaattaata cgcaaatcac gcaagtgctt actttcgttt attgccaagt gattcaaatg
     7141 atccgaaagg gtatcattcg ttaaattcaa atcacttatt aaattctgca aagtctcatt
     7201 ttctaatgct atcattttcc aaagaattaa gcttctacaa catcagaaat cgataatgtt
     7261 tcttcgccac gtttccagtt acacggacat aattcatctg tctgcaaagc atctaacaca
     7321 cgtaatacct cctccacgtt gcggcctaca tttaagtcat aaaccgaaac ccaacggata
     7381 attccttgag gatctacgat aaatgtagca cgataagcta ctttttcatt cgcttctaaa
     7441 atgcctaatt cttcggctaa agatttagat gtatcagcta acataggaaa acgtaagcct
     7501 ttcaaatcgg ggtggttctg acgccaagct aggtggacaa attcggagtc ggttgatgca
     7561 ccaattagat tggtatcacg atcgctgaat tcactgtaat tcttgttgaa ttcggcaatt
     7621 tcggttggac aaacaaaggt gaaatctttc ggccaccaaa acattaccat ccatttacca
     7681 gtaccgagtt gttgatcgag cgaaagagta gaaaattcct ttcctttttc gagcgaagta
     7741 actgatgtct tttcaaatgt tgggaaagac tctcccagag aaattatacg attcatgatt
     7801 tttgtttttc gttttagaca ggacaaaatt gaagcattcc taatcatcaa tcaaatcaat
     7861 aatatatatg atacaataga tattgtctat atttgcactc atgacactta ctcaactaga
     7921 atatctcatt gcggttgata ctcatcgaca ttttgcacgt gccgcggatg cttgctttgt
     7981 gactcaaccg accttgagta tacagcttaa gaagttggaa gatgaattag gagttgcttt
     8041 gttcgatcga ggtaaacagc ccgttgttcc aaccgatgca gccattcgaa tactggatga
     8101 agcccgacaa atggttcagc ttggcaagcg aattaaagag caagtgaaag atgaaacgga
     8161 ggaattacaa ggattaattc gaattggctt tattcctact gttgcgcctt atttattacc
     8221 gcttttctta ggagaatttc aagctaagta tccgcgagta gaattggaaa ttgaggaaat
     8281 caccaccgaa aacctcattg ccaaactcaa acgtgacgaa ctcgatgctg gcattgcagc
     8341 tacaccactt cccgaaagta atttacacga gctaccactc tattacgaac cctttgtggc
     8401 gtatgtggcg atgaaagatc cattattgaa aaaatcagca attcgcatct ctgatatcaa
     8461 cctttccaat ctatggttgt tagccgaagg acattgtttt cgatctcaag tattgaattt
     8521 gtgtcagatg caagataaat cgggtagcga tattcatatt catctcaaag caggcagcat
     8581 cgaaactttg aaaggattag ttgagcacaa cgccggcgtg acgctgcttc cagagttttc
     8641 cactcgtgga tttaacgacg aacaacgcga tcaattacgt tattttaaaa cacccgaacc
     8701 cgttcgtgaa atcagcttaa taacgtgccg tcaagtagtc aagcagcgct taactgaagc
     8761 actttgcaaa actatactat cggtgattcc cgagaaattt aaatcacccg taaaatcgaa
     8821 tattatccca atttaaattc aatattggtt tactgcattc attttcgaac gtaatttgtc
     8881 gcatgaaacg cacactttca attctaatcc cgctattagc agcgctactt tttggaatct
     8941 attatttcag caataaagaa aaaacacggt tttacaaaag acttccaatt ctcggaaacc
     9001 atagtatcga cagcacacgc agcggctcaa cagtcaaata cgacacactc tggcatcgta
     9061 ttcctaattt ctcatttact aatcaagacg gccaaccagt tactcagact gatgtgcaaa
     9121 ataaagtgta cgtggcagat tactttttca caacctgcca aagtatttgt cccataatgt
     9181 cgaaacaatt agatcgtgtg gcggctgcat ataaaaacga agctgatttt cgaattttat
     9241 cgcatacggt tgatcccgaa accgacactc ctgctcaatt aaaaggatat gctcaaagct
     9301 ataaggctaa tacgaaacaa tggattttcc ttacgggtga aaaaccggct ttatatgaat
     9361 tagctcgcaa aggttattta ttaaatctcg aagagggtga tggcggtccg gatgacttta
     9421 ttcacacaca aaacttcgct ctaatcgatc gtcaccagcg cattagagga ttttacgatg
     9481 gaaccgattc ggcacaggta aataatctaa ttcaggatat tggatttgtg ctacaagaaa
     9541 aataatacgt ttatttttta aagggcggtc gagcaggcta ttcgctccaa tatttttcac
     9601 cagctgaagc tttagcaaaa gcgggtgcta tccttaccgc tagcgtgaat ggtagatgat
     9661 tgaggtaata tggaagattc tcgattgatt ctggcaggtc cccggattga aatccggggg
     9721 tgctgttgtt tacttgggaa ttttagtttc aatttctatt ttaaaatccg ctcgcttcga
     9781 cagactcagc gagcgttcaa atagcagatt gagtcggtaa aatggggcac aaaaaaaggc
     9841 tgcatttagc agcctttttt attgaattaa attatgcgtt tctaaaattt aaaggaaagt
     9901 ccaacaccca aaacctcctt gaattgggtt ctcggtcctt tgccttctac ttctactcca
     9961 tttaccattc gttttacagg aacttgtata tcgttatcgt aaatcaaatt ggtaaaaatg
    10021 ctcgttgtaa gccatttgtt agctttaatt aggataagat tttcccagtt cacatcgata
    10081 ttagaacgtt gagcagcaat aggatctgta taattattaa acaactgaag gtttgaaaca
    10141 accgagatgt tttttactaa atccttttgc aaacgagcac gtaaataggc tccgaattca
    10201 aaacgagttt tctcacccgg agtaataaca gctccagtta atggatcaat tacagctgcg
    10261 gtaacaccaa attgacctaa atcagctaag cgttgatcca tcacgaaagt tgctcttccg
    10321 gttgcaggcg aaatgaacat cgaaaaccaa tcattcggtt tataatccat cccgagagca
    10381 agtgataaaa atccaggtgc tagaaatcgg gaaattaccg ttgaatcatc tgggaaatta
    10441 aatcccggtg ccatttgagt acgaaagttc agcattcctg cgtaaaaaaa cttgcctttg
    10501 gctttctttc caaactttgc attgacttcg attttatctt cattcttgcg aagtgactgc
    10561 gacttagttg tgataaaacc aaagccacca tccagcaccg attcgaaata taaatcgttg
    10621 tgacggtatt tcacaaatgc attaaccata gctgtggaag acaaagcact ctcgccccca
    10681 gctgcccaat tggttaatga tacttgatta aagtttaacg caaaaaaccc accttttttc
    10741 cacccattaa cggatgtgtc ttcggctact tcgatttgag cttgaagtaa agtggatgaa
    10801 aatacaagaa aagcaatcag attaaaaatt cgtttcatgc atttccgtat taacgacgat
    10861 cttttaacaa gtcacgaatt tcacccaaaa gcacttcttc tttgcttgga gcaggaggtg
    10921 cagcaggagc ttcttcttct tttttcttag ttgcattcat tcctttaatc accatgaata
    10981 aagaaaatgc aatgatgata aacgtgaaaa ttgcttgaat aaacttacca taggcaatca
    11041 cagcttttcc aacagtgtag ctcaatccgg agaaatcaac tccgcccgtt agtactccaa
    11101 tgattggcat aataacatcg ccaacaagtg aatttacaat accgccaaaa gcgcccccga
    11161 ttacaacccc aactgcaaga tcgataacgt ttccacgcat cgcaaatgct ttaaactctt
    11221 ttaagattcc catggtatag atttattgga acagatttat ttcccccaat aatctaactt
    11281 tttttcagaa cgaacaaact acaactcact tgagaagcta tttaattatt tatcagccaa
    11341 ataaatagca caaaaaaagc cgcttcatta agaagcagct tgataaaaaa gattgaattt
    11401 cttatttaac tgttagcaat tcgctgtaac gcccttcgat gcttgtccat actaatgtgt
    11461 acgttcctgc agcaagatta tcaattgcat ctaattgaat agtttgttga gtagtttcta
    11521 tgtttttagc tacccgtttt gctcctaaca tgtcgtaaac ttctaaagta ccacgaccag
    11581 cttttggcaa taccacttga acattcgaac cattactagg atttgggaat actcgcattt
    11641 cagaacctgc taacgaattt gaacggttat gacgaagtat caaatttaaa cgacctgttg
    11701 ctgaacttgc tggatcgttt gttatttcta caggatacga tttaccttct tccaccttaa
    11761 caatcgtttt catcaaacga tcttcaagat aaaatactac atccgaatcg aaagattcca
    11821 attgagaggc ttgaatactg tacgaaccaa catcaccttt taagcgaatc ggaattatct
    11881 tgtcgcttcc tgctatatcg ggttttggta acgcatgcac cgaaagcgca tctccttttt
    11941 cattcatcaa tccaaaactc aatgtcatgc ctggcaattc aggtttgtga gcatctaatg
    12001 ctggatcaaa atcttctgat gcttcatgac ggaattctaa tacagcttca tcttgtactt
    12061 ccgaatcttg cttgttaatc aacattctta attgagattg cgctgctcca attctaaaca
    12121 ataaactatc aggaaactgg gatgttttaa tcgattcatg agctattaat gaaggattat
    12181 cctgagttac tttcatgaaa aatccttgtc ctggtccaat ataacgtgaa cctccattcg
    12241 ttgctgcacc tgggttagag gtattatatg aagcgtattg attaatacga ggattccaaa
    12301 tataaatcgt gctcgaaata cctgttttgg tccattctgg agagtcccaa tcaattggtg
    12361 aagcatatgg atttgccact agattccatc catttccatt atcaactggc aagcaaccac
    12421 ctgtacatgt tacaggtaaa ggaatatctc cttggtttgg atttcctttg aagtcaataa
    12481 tagctgcatt cggaatcgta ttcacttgta aattcacttg agaacggtca ccaagatgat
    12541 aaactaggaa acctgcacct tttggaagta cttcacttgt agaagctgga gatctccact
    12601 cataaccgtt tagtaatgat tcattgtaaa caaaaacatt cgtagtaaaa agggtcggat
    12661 tatcccaacc gtttaacccg ttacctggac catacaataa aatattgtct tttacttgtt
    12721 tgaattttat tcctccaata gtatcaacag gagaaccaaa gaaacgatta gaacgaattc
    12781 ctcctcgtgt ccaacgctga acagtcatat tccctgaaat ggatgctgtt gatggaattc
    12841 tctcaattcg tgctgttagt gattgagaac ttaacagcgt cataacttta ttgttattta
    12901 ttccatcagc atctaaaaca gccgagttta aaacttcgag cgtgttttta acagatgtat
    12961 tagcgataat cgatttagta ccaattcgac tcaccgtaag gttattgtat acaaatccag
    13021 taatatcctg attaccgtta ttgttataat taatcgtgtt attggcagtt gtatataaat
    13081 tcgaaacgtg aggattaaat cctcctgttg ctgcaccaat ttttatttca cctggagcaa
    13141 gtgtaatcaa gcgtccactt cctgcaatgc tcaatttatt gtagtgcaat gctggtactg
    13201 tttgtggccc aaatccgttg aaattaactg ttcccgttcc gcgtacaaat gtaccactca
    13261 tatttaaaga tgagccaata ctcattgttc cagtctgccc atgaaaaata ccagaatttt
    13321 gattgaaatt ttggctgatg acaagtgaac cacctgatgt gtttccgttt agaattccac
    13381 cattttgatt aatcgatcca ctaacaacta caattccact cgcacaatta aaacttccgt
    13441 tatttattgt aagagtacct aatgtattta cttgtccagt accgcatgta aaaacgccta
    13501 attcctgaat aattccagaa agtgcattca tcgaacctga attaactgcg aattcaccat
    13561 tattagttaa aacaccactc acatttaagg aaccactcat gttttgaaaa cgaccattgt
    13621 tgatcaattc tgaattcaca tttagtgcat cacttccgtt cattagaaat ccattcggaa
    13681 gaatttctaa atttccaaga acagcaactg aactagttaa ttttgcaaat ccgctaacat
    13741 gaataacagg taataataac ccttccaaga aaaaattccc tccggccatt ccaaaaacca
    13801 tttctggatc aaattcgatt gttgaatttt gatgactaaa accgtttcca aaatttacat
    13861 ttccaaaaag ctgaaaaaca cctcctgttt tatctacata aatagcaggt aaaattccaa
    13921 caccatcggt taatgatgta ccaaaaatag tttgagtaga accaccagta aatctaagag
    13981 ttgcggtgcc tgggtttact tcagtacaac gatagttagc aatatttaag ttcccataaa
    14041 tactgatagt tcctgtgttg atacgcatgg cacgattgga agcagatgcg tttgtgagaa
    14101 gcaaatctcc attcaccgaa atggttccat aaacttcgat ggttttatta tttacacctg
    14161 aagtattata gaattctaga ttattaaaaa atggatttcc taaaatacta taaggaatag
    14221 atgaagttcc gctcattcga acagtaccat acgcgtgatt aaatgttcca ctgattcgac
    14281 ggaaatttcc ctttacatca agtaaacttg taggtgcagt aaactcagca tcatttgata
    14341 attcaaaatt gccatttact aacatggaac acgatccagt tgttgcgatt ccgctcgatt
    14401 gaatccagtt tcctgtttga atttgtgtac tacctgtaaa atcaatagtt ccggaaataa
    14461 aattcacatc cgaaccaatc gaaagtaaag caacaccagc atttttactc aaggatgaag
    14521 tgccatctac tgttaatgca cccgctatat tcaaaacgcc acttccaaaa cttaaatctc
    14581 cattactaag atgtaaagct cctgtattta ctaatccggt tccaaaactt accgaaccac
    14641 ccgaaattgt taacgtttga gcaagagcaa aattagaatt tccggaagct tttgttatag
    14701 tagcgccatt aaatgaacat acaccatcaa ctactaaaga accagttcct aaattgacaa
    14761 ttcctccttg tattgttaaa gcatgtgcat taaatgtgga tgaaccaaaa tcgattgatg
    14821 aattattagc tgtgaatgat gcattgttat tcatataaac agcaccagaa gttttattaa
    14881 acgtggcatt gtcaatcgaa ctattcccat tcatatagat acttgatgta cctccattta
    14941 aaattgcatt gcttaattgt aaagctttga aagaaattgc tgcgctttga caattaaaag
    15001 ttccacctgt aatacttaaa gctccacttg gattgaaagt tccaacacct gcagtcttag
    15061 taaaagttcc tcccgattgt gaataagata cagacaccat aaatgttcct gaaccggttt
    15121 gaaatgaacc tccagtaatt tccattgccc ccaatacttc aactgttgaa ttaccaatgg
    15181 taacagaacc tccatcgatt ttcacgttaa gtaatgagca aattccccca gcaccttttg
    15241 tcaaggtacc tccatcaacc cataaacgac cagctacttt tagtttagat aatccggttg
    15301 aaatagttcc gcccgaaagg tgaagttcct gcaaaacagt taagattgca gatgattgat
    15361 taactccagt aattgttcct gtataactgg gcaaaaccga caaattccga acaatcacat
    15421 tcgatggaag tgtgcaggag atactgctta ctgtcccatc aaatactaca tcgtcagatt
    15481 gtgttggtgt tccagcaggt tgccagttag ctggtgtact ccagttgctt ccggacgaac
    15541 ccgtccaaat cttttctgca gcacttgctg ttcctgccac taaggcaaga atgaccggaa
    15601 gtatggaagt aaataatcga ttcatggtga tttagttttt ctgttgtgtt tttgtacgtt
    15661 caaagctatc gtggatgtta tagtctataa gagttcgaag gcaatgccac ttacagtgta
    15721 agaaataagt gtagtaagga atatttaata tatcaatcac ctaacaatca gaatattaaa
    15781 tcgcattcat cttgaccagt caagcaaaat aaatggggga ttttgggaga attgcataga
    15841 attcgtccgg aaaagctcat tttctgtact actcaaaaaa taaaatgaaa gcggatttta
    15901 ctgaggtagc gaaactaact gctctaaagt ccaggcgcgt ggtttctggt caaagaggat
    15961 ttttgtcttg ctccaagtgc tctgaaaaag ttccgattga cgatatcgtt ccaaagaggc
    16021 ttcatctttc caataactgt aagtgaaaaa atgtcccgga tcattaggtt cattcaacaa
    16081 ttccaaatgc tcacatccgt cgaaagctcg aatcttttcc ttactctgat gaaatacctc
    16141 gagaaattct tcccgcgcat ccaaacgaaa atgcatctca acgattcgta tcaacatgga
    16201 attctacgcg taaggtgtcg ttaactttta atcctatcaa cttagctgct gaacctctgt
    16261 taatcgatat ttccaagtat cccgatgcat tgaataatgc acacatatct ccttcgggaa
    16321 cttcattgta actcttcgat atacgaacaa tattttgcga agctcgacgc aaaacaacct
    16381 cgaattttcc tccttggcga cgttcctcga attgctcctg acgaatattg gtaatgaggt
    16441 ttccgtatga gtcgatgtaa atgatactcg cacctaaatg cgattcacct cgtgtaggcg
    16501 ataacatcaa actttggcgg aatccagaaa tcttgcgacc aattatctct aacgttcctc
    16561 ctcgagcaat gtgacaggca gcggtaacga ataaatcaac tgtgggaaat gacttaggag
    16621 tttgttcttt ccgaatgttg agttgaacta tctcatcggc ctgttgagga gagaaaagca
    16681 aggaaaaaat acccgaatcg gctcctacga aaaaatgtcc atttatcttg agtgccacat
    16741 gctgtgtatc ttcatcttcg agtgtttcca caccaacgat atgaaccgtt ccaggaggaa
    16801 aatgcgtata agcatttcga aggatgaaac cagctttcat gcgatcgaac ttgggaatct
    16861 catgcgaaat atccacaata gtagcattcg gcaattgagt taaaatagca ccctttaccg
    16921 cagcaacgta ataatcgctg agtcccatat cggtggtaag ggtgatgatt gccatagttt
    16981 caaattcgat gcttggagag acgagccaaa attcatttat ttgtgtggga aatgcaacca
    17041 tgcatttcag caattgataa caattatcct tttctagttt gatcgaaaaa acaatccatc
    17101 tcccggacgt taatccagtg gagatttacg gcgttaatga tgaaacatta gacttgattc
    17161 gcgcctttta tcccggcttg cgacttgttg cccgtggctt agatttaaaa attcagggag
    17221 aagaacccga aattgctgcg tttgagacga aactaaatcg actcatttcg caccaagtac
    17281 aatatggtag cttaagtgaa cagttagtaa agaagctgat gaaccccgaa aatgatcttc
    17341 ccgaaatgac tcctgaccaa aatggagctt cggatgtgat tgtttttgga aacaacggtc
    17401 tcatcgttaa agcacgaact ccaaaccaac gtcgtattgt aaatgcttcg tttactaacg
    17461 atatggtttt tgcaattggt ccagctggta caggaaaaac ctacacagca gttgctttgg
    17521 ctgtgcgtgc gcttcgcaat aaagaagtaa aacgaatcat cttaactcgt ccagctgttg
    17581 aagcgggcga aaatctagga tttcttccgg gtgatttgaa agagaaactc gatccctatt
    17641 tgcaacctct gtacgatgcc ctcctcgata tgattcctgc cgataaattg cagctattcc
    17701 tcgaaacagg tatcattcaa attgctccac ttgcctttat gcgtggtaga acactcgata
    17761 atgcttttgt gattttggac gaagctcaaa ataccaccga agctcaattg aagatgttcc
    17821 ttacacgtat gggacgttct gccaagtttc ttatcacggg agatttaact cagatcgacc
    17881 ttcctcgtaa tcaatcgtct ggtttagcga ccggaattcg cattttaaaa gacattgcag
    17941 gtatcgaaat catccattta gacaccagcg atgtgattcg tcataaactc gttactgcta
    18001 ttcttcatgc ctaccaagcc aaccagccag ataagcctgc gcgcgactga ttttcatttc
    18061 gaaggacaaa ccgctttcta ccgtggaaaa gtgcgtgatg tatacaccat taaaaatcag
    18121 tacttggtga tggtggcaag cgaccggatt tcggcgtttg acgtggtgct accaaaggga
    18181 attccgtata aaggacaagt gctgaatcaa acagcaactt atttcttaga agccactcgc
    18241 gacatcgttc ctaattggct tttaggaact cctgatccga atgttgcagt aggaaaaatg
    18301 tgcactcctt ttaaagtcga aatggtgatt cgtggctacc tagccggaca tgcagctcga
    18361 gaatatgctt tagggaaacg tgtaatttgt ggtgtttcat tgccggatgg gttgaaagaa
    18421 aacgaccgtt tacctgaacc gatcatcaca cctacaacga aagctgccga aggacatgat
    18481 gaggatatat cacgtgagga aattttgcgt cagggaattg tggcggaagc cgactatgtg
    18541 ctattagaaa aatacacgca agccttattc aaacgtggct ctgaaatggc cgccgaacgt
    18601 ggattgatac tggttgatac gaaatacgaa tttggcaaaa ctgattctgg ggagattctt
    18661 ttaatagatg aaattcatac tcccgattcc tcccgctatt tttatgcaga tggctatgcc
    18721 gaacgacaag ctcgcggcga agttcaaaag caactaagca aagaatttgt gcgacaatgg
    18781 ttaattgcaa acggtttcca aggaaaagaa ggtcagcaaa ttcct
//
LOCUS       DAOOQW010000029        18123 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_3769_length_18123_cov_5.039462, whole genome shotgun sequence.
ACCESSION   DAOOQW010000029 DAOOQW010000000
VERSION     DAOOQW010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 18123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18123
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3769_length_18123_cov_5.039462"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="PK281_03295"
     CDS             complement(<1..290)
                     /locus_tag="PK281_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010180843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 family protein"
                     /protein_id="HOY48398.1"
                     /translation="MIHINFPVLLISALIPLIVGFIWYNPKLFGTVWMKEAGLTTESL
                     AGANMLKIFGFTYILSVLAAFILQFMVIHQFGVYSVLAVQPGINDATSEMGL"
     gene            complement(338..877)
                     /locus_tag="PK281_03300"
     CDS             complement(338..877)
                     /locus_tag="PK281_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006262111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOY48399.1"
                     /translation="MSSYRFSIETDRLLLREMREEDALAFYQLNADPEVLKYTGDDSF
                     EDVEQAREFLQNYPQISYLKDGYGRWTCVLKSSNEIIGWCGLRKQDTGETDLGYRFHQ
                     RFWGQGFATESSIKSLQIGFNELGLDRIIARAADENLASIRVIQKMGMRFLKNEELHG
                     QASSIYELFKHDWDLYHRC"
     gene            complement(874..2187)
                     /locus_tag="PK281_03305"
     CDS             complement(874..2187)
                     /locus_tag="PK281_03305"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOY48400.1"
                     /translation="MKKLLPLLLFWLLANNLEAQNMYFPPLTGTTWQTITPAELGWCE
                     DSIPALIDYVGENNSKAFLLLKDGKMVIENYYGTFTRDSLWYWASAGKSLTGFMVGMA
                     QEQGLLNINNPSSNYLGTGWTNETPQQESQITVRNQLTMTSGLDDGVNSPDCTIDTCL
                     IYKADAGTRWAYHNAPYTLLDGVMEGATGNTVNQFYSNNVRNRLGMGFSAYIQTGENN
                     VMYSTARAFARFGLMILNRGVWNQDTLMHDMSYFDAMTNTSQNLNLSYGYLWWLNGKA
                     SFMLPGSQLVIPGSLMPNAPDDMFSALGKNCQYLNVVPSKGIVLVRMGNDPNGLGGLV
                     PTVLDNEIWRRLNLIMCDIPTSINPSEKELSFKVFPNPTSDRIQVQTSDLSNVELLEL
                     VDLQGRILTKSNTNELSVLDVSTGSYFLRITGHKGISVMKIQVNR"
     gene            complement(2212..4035)
                     /locus_tag="PK281_03310"
     CDS             complement(2212..4035)
                     /locus_tag="PK281_03310"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOY48401.1"
                     /translation="MKITTWSLFVLFLFGFEGISHAQKTPSLLKQADSLIQLGNYAQA
                     IPLFTQLIQLEPKNEKAFSGRGLANIKLGNLAAGKSDFQQAIVLNPACSNCYLTLAQL
                     AAQSSNLKETETLINKAIQIDPKNENAFLMRAELREAQKDYAGSLRDFDQAIVLKPNI
                     PESYIERAGLNERSGYEALAKADYALAIEKAPKQASVYFSRANFFIKQTQWKRALDDL
                     DMAISLDSTQAEYHSYRGSVLYYLDEFNPSIIAYSRSIQLDPTNYRTFIYRGMSWHKL
                     ENMDSCCFDSQQAMKLIPSTDTAEILKLQTQINDLCNQNQSSFFYQRGIATYNLGKFE
                     DAIQFYNRGLEKFPTSGKLISFRGNAYKAQGNNELALKDYLHVCTLKKEFQNEIKEDP
                     MYVNNPSFSQIYYIGSIATLYESIAECYSEMGQYDQALTYADSAVNTVVSWSKNENLK
                     SVYVTCLTIRGALYNEKEELVKAKSDLVMACTISSNNPLPHINLAITYIKEAFQKNNK
                     VKTIQINLGNLRGAGQLNLPAQTNKNAQLDILQKALQESNLAINLNPQLAFAYLVRAH
                     IKNILQESDVCLDALMAEQYGIPDVPAQLGISCPNNTPIKH"
     gene            complement(4314..5348)
                     /locus_tag="PK281_03315"
     CDS             complement(4314..5348)
                     /locus_tag="PK281_03315"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding domain-containing
                     protein"
                     /protein_id="HOY48402.1"
                     /translation="MIELRLLEIVRIELETPSTKSIYLKPIDGKPLHFIAGQFLSLVF
                     ESGEHAFRRSYSISSLPGDEWIRITVKRISNGNASRQIFDHWQIGQHVQTLEPYGRFV
                     LPEITQAQDFFFIAAGSGITPIISLIREALLRFPHVKIHLLYSNRSRSECIFLNELQK
                     LQEYQPQRLQIHWFFSDAQAISQARLQSDWLNNYLKKTSQSESHVFTCGPESFMYLAE
                     VVSLSEGIPIQHFHQELFYQEEEPEIRNWGTSQYGVSLTYKNKRYDWQIPANQTILSF
                     AKEQQVELPWSCEAGRCSTCMATCISGTVEMTRNEVLTDSDLAAGKVLLCTAHPQSDH
                     IVLKVPSYTN"
     gene            complement(5363..7135)
                     /locus_tag="PK281_03320"
     CDS             complement(5363..7135)
                     /locus_tag="PK281_03320"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY48403.1"
                     /translation="MKKLLFLLAILASTFSSKSETVIQCMNRVNHEVLKQLDLPASWS
                     VETVQTANFNEAIQLHLSHVIQVLSARNISTLTTTQKANRKHLLNVLAIYGQAGKFPI
                     NEHAPYQTPVFIDHYNTHCAVGYLMEQSGAETLAQEICRKQNLAYVREIQVKGVAEWA
                     SLNGFTVDELAWIQPGYPPTTTVTPLMGGTNNDVFDIINLNGELIAAGAFTQADNQPA
                     AHIASYISGFAGWFWMETSGGTNGPVYALDTINHQLIAAGNFTQAGSQTVANVTQMVD
                     GFWMPMGSAINGTVYDLEWHEGTLYAAGEFTLPGGSNLAIWNGSEWEALSFSPNGPVR
                     CLKTTADGLIYGGDFTQSGSVTTNHIGKIFSGMPEAIGNGIATIVYDVENWNNKPIAA
                     GAFKENGQVYGMMQFNSGNWQSLTGMNEIAQLDTTGVIYSLKLMSNVLYVGGEFDQYD
                     LMTFGRSLMKWSSPDEFPTPIAVFDGTVYCLYANQDILYVGGSFTHHLGTEINHIAQL
                     VDLVGIEPITGKSGQISAYPVPSGEDVNLQNAGSDMHMKLYSIQGKLIQEFQLKKTET
                     IHLPAAGVYILQADNGELLRLVRQ"
     gene            7332..8204
                     /locus_tag="PK281_03325"
     CDS             7332..8204
                     /locus_tag="PK281_03325"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY48404.1"
                     /translation="MKTTYFLFLSILFSPIFLSAQSIPNGGFENWSAPNAYNVPDSWG
                     NLNVLTSEFSVYTCTKGSPGSAGAAFLKLTTKNIPGVGVINGIAVSGNLNTTDMSTDA
                     GFPYSERPISLTGKWQYMGFGADMGIVAVYLTKWNSLTTQRDTVGGIFHPLTGMEMSW
                     ASFSLPIVYLSNETPDSCLIALASSGENPVANSYLYVDELAFELNSSGFNTVQSSDFI
                     LYPNPAQTELTLELPASSAHIRFFDFSGKLIQQQTTNGNSRQTINLIDFAPGVYWVQV
                     QTKQGISTSRFIRQ"
     gene            8304..10154
                     /locus_tag="PK281_03330"
     CDS             8304..10154
                     /locus_tag="PK281_03330"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HOY48405.1"
                     /translation="MFLRRVLIPILLLIVHSSLAVNTPERVEPANWWIGMKSQFLQVL
                     VKASRVAQMDVQFNYPGLRLLEIHPGDHPDYLFLDFEVSDQMQAGDCELNFYNPQIKG
                     KKQYQYTYRFLKREEGSANRKGFSGKDVIYLICPDRFVNGDPSNDSKPDMADKLNRND
                     PSGRHGGDIQGIIQSLSYLQQLGITSLWINPLVENNMPQHSYHGYAITDYYKIDSRYG
                     DLTLYLKLVQEAKLRGMTIIADQVANHCGSSHWWMKALPFKDWINYPDSAQFTNHMRS
                     TLQDPHAVAADRTLFSDGWFVPTMPDLNQRNSYVANYLIQNSIWWVETAGLGGIRMDT
                     YSYSDKAFMNLWSKRLMDEYPNFSIVGEEWSENPAIVAQWQAKDLIEPGKSALPSLMD
                     FPLQAALVKALTQPENWNSGWILLYEALANDFLYPHPEKLVLFGDNHDMDRFYSQLGA
                     NPALFRLGMVYLLTTLRIPQIMYGTEILMQNEKHNNHGEIRSDFPGGWRGDSVNVFTN
                     QGLSTEQIQAQQFIIRLTSWRKASEVIHSGSMVHFAPKNGVYVYAREKGNNSVLVLLN
                     KENTPITINLKRFSSIIRDRKIAWDVLKDQEMTVGDELILSPVSAKIIEF"
     gene            10195..11082
                     /locus_tag="PK281_03335"
     CDS             10195..11082
                     /locus_tag="PK281_03335"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOY48406.1"
                     /translation="MIRLAIVFCLCTMTLSAQKKVPGNGRIQRFPHFPSNQVEPRNID
                     VWVPEDYPQKGPYSVIYMHDGQNLFDSTTAYGNIPWKAGEAASALLRDKKIAQCIIVG
                     IHSNDKRFLEYNPQSIFKTLPATLQQKLVAEYQGEPISDAYLLFIVKELKPFIDNTFY
                     TSRVRENTCIAGSSMGGLISLYAMCEYPKVFGSAACISTHWPVSLKMNDARIPKAIKA
                     YLNKKLPPSTEHRVYFDHGTETLDKQYAPYQKEIDALMKEKGYSKKNWLTKVYSGAAH
                     SELYWQKRFPAVLKFLLPR"
     gene            complement(11083..11916)
                     /locus_tag="PK281_03340"
     CDS             complement(11083..11916)
                     /locus_tag="PK281_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48407.1"
                     /translation="MTSRLSGIATAFLLLVLLNSCRKEELDPITYPSKSVDIAELEIR
                     TSDMGKAFTFWSETLGMPIIDTNTVSFTVKIGSSRLTYRLSNTTLPPVNHFAISIPGN
                     QIEKALDWLKNTDGKYANGPKNPVEIITDEITGAEISSNPIYNSHSIYINDGTGNIIE
                     LIARHDFGTNVTGDFNQDQLLKISEVAVVTKNITKCLGIVSEEFGVDVFPNSTSSYKP
                     VGGADGIILLIMPNKPWKPTESILSEPFETIVTLRYPEDKTILLPNMVTYSTFTIKTE
                     P"
     gene            complement(11943..13511)
                     /locus_tag="PK281_03345"
     CDS             complement(11943..13511)
                     /locus_tag="PK281_03345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48408.1"
                     /translation="MRRIFLLFILFIPALAQAQVPSKPQPAFRDTIKPRKDTMRVDTT
                     EFWRDSLFKDPKQRVLKDGAVLRPPRPPRKIRKFKIRYSDRIQYNVLLDWPVTASAGV
                     PSVLTLQKQLTSWEDSLLPIRWKGDTTASQRAWSVVYRFGKIFLLDAPIESLLLSAEE
                     DFFGSMGRSREFQLKGINYTFAPPYPLALWKPSGGIKYDQTTLENEASRMQLAQLSGA
                     ALDAGSMASDQLSLRWMQRKSVNYRESLHLLRANLAGLGSVMSLSDNPEFGNTAAANW
                     LYYTNRQYGFYSEYRYKAADLKRDYLLATFTNPNVYTALYSVFYKYMITGEDSMATPA
                     IKLGYGKYVLPWARLGFTPFGPEWIPSLTLTNHRQMLQAYARIGTGNFAESYGGGIKL
                     FNIKRSQNFTLNAHVSIWKQRYFFQDWLTQQVQPINWGGAAIVSGNLLLTKSWNHPMS
                     LAFQAGYKTRGYLEGEVWEASPVIRVGLSFALDRDYSQDDTVPEYFTPVKKKKLTKSQ
                     KRKAAIAKKKVQRK"
     gene            complement(13605..14291)
                     /locus_tag="PK281_03350"
     CDS             complement(13605..14291)
                     /locus_tag="PK281_03350"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HOY48409.1"
                     /translation="MKVTLGAQDWFIVVQDSFSAIANPSKAVAMSAYMKYQFKYLGIQ
                     KPLRAEATRNLFSLYEKPSLEVLDELVPLLWESQFREYHYFAMECLFHQRKHFRLTDI
                     DFLESLMRKNSWWDSIDYLSPVLAAAYFKKYPSYLIPISKRWNKDKNFWIQRASLLVQ
                     LKYKKESNWELQFDLIQNLMHEKEFFIRKAIGWSLRELAKTDAEAVRDFVLSNPLSAL
                     SKKEALKHIY"
     gene            complement(14370..14900)
                     /locus_tag="PK281_03355"
     CDS             complement(14370..14900)
                     /locus_tag="PK281_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006257291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HOY48410.1"
                     /translation="MSTTKWVLDPTHSELQFKVKHLMITTVTGHFGVLESEVTTEDND
                     FSTAKISFKAAVDSINTGNEQRDGHLKSDDFFSTEKYSHLVFESTQIEKKNEEEFTIH
                     GNLTIREVTLPISLKAELGGIAKDPWGNLKAGFTVSGVINRKDFGLTWNAVTESGGLL
                     VSEDVRLAAEIQYVKS"
     gene            complement(14924..15643)
                     /locus_tag="PK281_03360"
     CDS             complement(14924..15643)
                     /locus_tag="PK281_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HOY48411.1"
                     /translation="MNNTIIHRATTRGHADHGWLNTHHTFSFAQYYNPERMNFGVLRV
                     LNDDIVAEGKGFGTHPHDNMEIISIPLAGDLEHKDSMGNVQVIRENDVQVMSAGSGIT
                     HSEYNRNSDKQVNFLQIWVFPNKRNVKPRYDQLSFPKADRHNQLQQILSPNADDEGVW
                     IHQDAWFYLGSFDSTFSSSYALKKPGNGIYLFVLEGSIQMGDTVLNRRDGMGVWDTPS
                     IQFQSLEKTEILVMEVPMQLA"
     gene            complement(15942..>18123)
                     /locus_tag="PK281_03365"
     CDS             complement(15942..>18123)
                     /locus_tag="PK281_03365"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOY48412.1"
                     /translation="EYTQAGVCSYGNNGCTAPYIVPDPSWTPPAPMCSGAATIDLNTL
                     VTGTPGGYWSGPGVTGHNFSPAGLSGDVDITYTFSPNCADAVSETETISIVSGGSAAW
                     TPQTVCSGAGTVNLNTLLTGTSGGTWSGTAVTGNTFDPAGLSGNISITYTVGTGTCQA
                     TSTQNINVVASANPAWAFAQSSVCASDPAINLNALITGTTGGTWTGNGVTGSSFSPTG
                     LSGNQTITYTVGTGACVQTSNQTINVIANSDASWTATSICTSTSSLDLNTLVTGTAGG
                     TWSGTGVTGNLFNPAGLTGPQAITYTVGAVACLSTATHDITVITQPVAPLINGTNTFC
                     SGTAITPLTATPAAGSTITWYSDAALTTSVGTGNSYTPPAATATYYATQGVTGCQSPA
                     SSFSITLNQSPQPPVLPAQVQWCAGTPLPILTAVSTAPIITWYSDAAQTTQIGTGLTY
                     PITSSSNASYFVTAENLGCVSAPASTNFIAEPLITAQILGNSLVKACLPKVITFQSNS
                     PTGNSWSNGSNQSSISVVQAGVYVLTQQGFCNTSTDTVTLVDMSVNAAFNVEINETGL
                     LPIDVVVVPLGNEADHCTWFLNGLDYELADNNTFTFTEEAQQTITHICNNQFGCADTA
                     TQVVVIELEPELYVPNSFTPNGDNINDVFKISGYEITQLNMRIYDRWGEEIYVITTPD
                     GSWDGTMSSGKLVPDGVYVYHLNARDKHSKPYHFHGSVLLIRYK"
BASE COUNT         5373 a         3559 c         3559 g         5632 t
ORIGIN      
        1 tacaatccca tttctgatgt agcatcgttg attccaggtt gaacagcaag taccgaatac
       61 accccaaact gatgaataac catgaattgc aaaatgaatg ctgctaatac actgagaata
      121 taggtaaagc caaagatttt gagcatgtta gcacctgcga gggactcagt agttaagcca
      181 gcttctttca tccaaactgt tccgaaaagt ttaggattgt accaaataaa gcctacaatt
      241 aacggaatga gagcagaaat taggagtaca ggaaagttga tgtgtatcat gggtatgggt
      301 ttttttcaaa gatatatttt ttcaattcga atcaaaatca acaccggtga tacaaatccc
      361 aatcatgttt gaacaattcg taaatactac ttgcttgacc gtgaagctct tcattcttta
      421 aaaagcgcat acccattttc tggattaccc gaatcgaagc taagttctca tctgctgcac
      481 gagcaataat gcgatctaat cctaattcat taaacccaat ctgcaaactt ttgatggatg
      541 attcggtggc aaatccttgt ccccaaaaac gctgatgaaa tcgataacct aaatcagttt
      601 cacctgtgtc ttgcttgcgt aatccgcacc atccaataat ttcgttactc gattttaaca
      661 cacaagtcca tctaccatat ccatctttca aataggagat ttgaggatag ttttgtagga
      721 attctcgtgc ttgttcaaca tcttcaaaag aatcatcgcc cgtgtatttg agcacttctg
      781 gatcagcatt caactgataa aaagctaagg catcttcttc tctcatttcg cgaagaagca
      841 agcgatctgt ttcaatggaa aatcgatatg aactcatcta ttcacttgta ttttcattac
      901 cgaaatgccc ttatgtcctg taattcgtaa gaaataactt cccgtactaa catccaaaac
      961 ggaaagttcg tttgtgttgg attttgttaa gatgcgacct tgtaaatcga ctaattcgag
     1021 cagttcgaca ttcgacaaat cggatgtttg tacttgaatt cgatcgctcg ttggattggg
     1081 aaaaacttta aaactcagtt ctttctccga tggattaatg gaagttggaa tatcacacat
     1141 aattaaattc aagcgacgcc aaatttcatt gtcaagaact gttggtacta aacctcccaa
     1201 accatttggg tcatttccca tccgaactaa aacaattcct tttgatggta ccacattcaa
     1261 atactgacaa tttttcccca atgctgaaaa catatcatct ggcgcattcg gcatgagcga
     1321 acctggaatg actaattgcg aacccggcag catgaaggaa gctttaccat tcaaccacca
     1381 caaataccca tacgataagt tgaggttttg cgaagtattc gtcatggcgt cgaaatagga
     1441 catgtcgtgc atcaacgtat cttgattcca cacaccgcga tttaaaatca tcaagccaaa
     1501 acgcgcaaaa gcccgagcag tgctgtacat cacgttattt tcgcccgttt gtatgtaggc
     1561 gctaaaaccc attcctaatc gattgcgaac attattgctg taaaattgat ttacggtatt
     1621 gcccgtggca ccttccatta ctccatctag caaggtataa ggtgcattat gataagccca
     1681 acgagtgccc gcatcagctt tataaataag gcatgtatca atcgtacaat cgggactatt
     1741 aacgccatca tctaaaccac ttgtcatggt taattggttg cgaacggtaa tttgtgattc
     1801 ttgctgggga gtttcgttcg tccagccggt gccgaggtaa ttgctactag gattattgat
     1861 gttgagtaat ccttgttcct gagccatccc aaccataaat cctgtgagcg attttccagc
     1921 agaagcccaa taccacaggc tatctcgtgt aaaagttcca tagtagtttt cgataaccat
     1981 tttaccatct ttcaacagta agaatgcttt ggagttattt tcacctacat agtcgattaa
     2041 ggctggaatc gaatcttcac accaacctaa ttcggctggg gtaattgttt gccacgtagt
     2101 tcctgttaat ggagggaaat acatgttttg agcttctaaa ttattagcaa gaagccaaaa
     2161 taggagtaaa ggcaggagtt ttttcatggt gctagagact aaataaatag tctaatgttt
     2221 aatgggagta ttattcggac acgaaattcc taattgagct ggaacatcag gaataccgta
     2281 ttgttcagcc ataagtgcat ccaaacacac atccgattct tgtaaaatgt ttttaatgtg
     2341 agcacgaacg aggtaggcaa atgctaattg aggattcaga ttgattgcca gattactttc
     2401 ttgtaatgct ttttgaagga tgtcgagttg tgcgttttta ttggtttgag caggtagatt
     2461 caattgtccg gcacctcgta aattccccaa attaatttga attgttttta ccttattatt
     2521 tttctgaaaa gcctctttga tataggtaat agccagatta atatgcggta gtgggttgtt
     2581 gctacttatt gtgcaagcca taactaaatc ggattttgct tttacgagtt cttctttttc
     2641 gttgtacaat gcacctcgta tagttaaaca ggtaacataa accgatttta gattttcatt
     2701 cttactccat gaaactacag tattaacagc actatcggca taggtaagag cttggtcgta
     2761 ttgtcccatt tcagaataac attcagcaat cgattcatac aaagtagcaa tcgaaccgat
     2821 gtagtagatt tgactaaagg aaggattatt cacatacatc ggatcttcct tgatttcgtt
     2881 ttggaattct tttttcaggg tacaaacatg taggtaatct tttaatgcta gttcattatt
     2941 tccttgggct ttataggcgt ttccgcggaa ggagataagt ttaccacttg ttggaaattt
     3001 ctctagacct cgattgtaaa actggattgc atcttcaaac ttcccaagat tataagtggc
     3061 tattccacgt tgatagaaaa agctactctg gttttgatta catagatcgt ttatttgtgt
     3121 ttgaagcttg aggatttcgg ctgtatcggt ggagggaatt aatttcatcg cttgctgtga
     3181 atcaaagcaa cacgaatcca tgttttcgag tttatgccaa ctcattcccc tataaatgaa
     3241 tgttcgataa ttagtaggat ccaactgaat tgaacgagaa taagctatta tggaaggatt
     3301 gaattcatcc aaataataca acacactacc gcggtatgaa tgatattcgg cttgtgtact
     3361 atcgagtgaa atagccatat ccaaatcatc caaggcacgt ttccattgcg tttgtttgat
     3421 gaaaaagtta gctcgagaga aatacacact agcttgttta ggagcttttt caattgcaag
     3481 tgcataatcg gctttagcaa gtgcttcgta acccgagcgt tcgttcaatc cggcacgttc
     3541 gatatatgat tcaggaatat tcggtttgag tacaattgcc tgatcgaaat cgcgtaaaga
     3601 acctgcataa tctttttgtg cttcccttaa ttcagctcgc attaaaaaag cattttcatt
     3661 tttgggatct atctgaatag ctttgttgat aagcgtttcg gtttctttca gattactcga
     3721 ttgggcagca agttgtgcta aggttaaata gcaattacta caagcaggat tgaggacaat
     3781 tgcttgttgg aaatctgatt ttccagcagc taaattcccc aatttgatat tcgctaaacc
     3841 tctaccacta aaggcttttt cattcttagg ttcaagttga atcaattgag taaaaagcgg
     3901 aatagcttgt gcatagtttc caagttgaat aagagaatcg gcttgcttga ggagcgaggg
     3961 agttttttga gcatgcgata ttccttcgaa cccaaaaaga aataaaacaa acaaactcca
     4021 ggttgtaatc ttcatgctgc aagatattca gttaagtcca taaaaaaagc agccttataa
     4081 gactgcttga ttcacaaact aaacgaaact taacatgaat gataaactac tttaattcag
     4141 gcttcgtatc ggaaacaaat cctttcggtt aaattggaat gtctccgatc cgaaatccca
     4201 tctacatagc aaagatgagc ttgggaatta actctaaagt caagtctagg ttaaggcttt
     4261 atgagaaaat tggaagtgtc gtattaacga aatcttggag tatttcttcc agattaattc
     4321 gtgtaactcg gaactttcag cacaatgtgg tcgctttgcg gatgtgccgt gcatagcaat
     4381 acttttcctg ctgctaaatc gctatcagtt aggacttcat ttcgagtcat ttctactgtt
     4441 cccgaaatac aagtagccat gcaggtgcta catcgtcctg cttcacacga ccaaggtaat
     4501 tccacttgct gttctttggc aaacgatagt atggtttgat tggcaggaat ttgccagtcg
     4561 tagcgtttgt ttttataagt aagagatact ccatattggc ttgttcccca gtttcgaatt
     4621 tctggttctt cctcttgata aaatagctct tgatgaaaat gttgtatcgg tattccttcg
     4681 cttaaggaga ccacttcggc gagatacata aaactctcgg ggccacaggt aaaaacatga
     4741 ctctcagatt gactcgtttt tttcaagtaa ttattcaacc aatcgctttg caaacgtgct
     4801 tgagaaattg cttgcgcatc gctgaagaac cagtgaattt gtaatcgttg aggttgatat
     4861 tcttgaagtt tttgtagttc attcaaaaag atacattccg atcgagagcg attgctatac
     4921 aacaggtgaa tcttcacgtg tggaaatctc aaaagtgctt ctcttattaa gctaataatg
     4981 ggagtaattc cgcttccggc agcaataaaa aagaaatcct gagcttgcgt tatttcgggt
     5041 aaaacgaaac gtccgtatgg ttcaagtgtt tgcacatgtt gaccaatctg ccagtgatcg
     5101 aaaatttgtc gggaagcatt tccgttgctt atgcgcttta cagtaattcg aatccattca
     5161 tcacctggca gagaagaaat tgaataggaa cgacggaaag catgctcgcc tgattcgaaa
     5221 acaagactga ggaattgtcc ggctataaaa tgcaacggtt taccatcaat tggtttgaga
     5281 tagatactct tagtagatgg agtttctagt tcaattcgta caatttcaag caatcttaat
     5341 tcaatcaaga ggcagacttc atttattgtc taactagacg taacagttca ccattatcgg
     5401 cttgtaaaat atacacgcca gctgcaggta aatgtatggt ttcagttttt ttaagttgaa
     5461 attcttgaat gagtttacct tgaatggagt agagtttcat gtgcatatca gaaccggcat
     5521 tttgcagatt cacatcttca cctgaaggga cagggtaagc tgaaatttga cctgattttc
     5581 cagtaatagg ttcaattcca actaaatcta ctaattgtgc gatatgattg atttcggttc
     5641 ctaaatgatg ggtgaatgag ccacctacgt atagaatatc ttgatttgca taaaggcaat
     5701 aaacggtacc atcaaacacg gcaattggag ttggaaattc atctggacta ctccatttca
     5761 ttaggcttct gccaaaagtc attaaatcat attgatcaaa ttcgccacca acgtataata
     5821 cattcgacat gagtttaagg gaatagatca cgccagtagt atcgagttgg gcaatttcgt
     5881 tcatgccagt taatgactgc caattaccgg agttgaattg catcatacca tatacttgtc
     5941 cgttttcttt aaaagctccg gcagcaatag gtttattatt ccaattttct acatcgtaaa
     6001 cgatagtagc aataccattt ccaatagctt caggcattcc gctaaatatt ttcccgatgt
     6061 gattggtagt aacagagcct gattgcgtaa aatctccacc gtaaatcaat ccatcagcgg
     6121 ttgttttaag gcaacgaaca ggaccattcg gactaaaact taatgcttcc cattcgctac
     6181 cattccatat agccagatta cttccgccag gcaaagtaaa ttcacctgct gcatataaag
     6241 ttccttcatg ccattcaaga tcgtagacag taccgttgat ggcagatccc attggcatcc
     6301 agaagccatc taccatttgt gtcacgttag caaccgtttg actaccagct tgagtaaagt
     6361 tcccagctgc aattagttga tgattaattg tgtcgagggc gtaaactggg ccgtttgttc
     6421 cgccagatgt ttccatccaa aaccaacctg caaaccccga aatgtaggag gcgatatgag
     6481 ctgcaggttg attatcggct tgtgtaaatg ctcctgcagc aataagttca ccgttcaaat
     6541 tgatgatatc aaaaacatca ttattcgttc ctcccatcaa aggagtaacg gttgtagttg
     6601 gaggataacc aggttgaatc caagctaatt catcaactgt aaatccgtta agagaagccc
     6661 attcggccac gcctttaact tgaatttcgc gcacataagc gagattttgt ttgcgacata
     6721 tttcttgagc gagagtttcg gctccgcttt gttccatgag ataacctaca gcacaatgtg
     6781 tattgtaatg atcaataaac acaggtgttt gatagggagc atgttcgttg attggaaatt
     6841 tacctgcttg accatatatc gctaaaacat ttaataaatg cttacgattt gctttttgcg
     6901 tagtagtaag tgttgagata tttcgagcac ttaatacctg aattacatgc gaaagatgca
     6961 actgaattgc ttcgttaaag ttggctgttt gaacagtttc aaccgaccaa gaagctggta
     7021 aatccagttg cttcaacact tcgtgattca cacgattcat acattggata acggtttcgc
     7081 ttttggaaga aaaagtcgaa gcgagtatgg ctaggagaaa gaggagcttt ttcatgggta
     7141 ggaaatgata gggaaattta gttttttttt atcatttcat ttaatgcatt tgtgatcaat
     7201 atgaaataaa taggaaaaca acagaattat aattctgagt aatttagttt tctcaagatg
     7261 atcaattagc attcaaaatt aaagaggttt tgcaattgat tgattcatat tttttcatca
     7321 aacgttgctt tatgaaaaca acttactttt tatttctaag tatccttttt agccccattt
     7381 tcctttcagc ccaatctatt ccaaatggtg gtttcgaaaa ttggagcgct ccaaatgctt
     7441 ataatgttcc agattcttgg ggcaatctca acgttttaac ttccgaattc agtgtttaca
     7501 cctgtacgaa aggctctcca ggctctgctg gtgcagcatt tctgaaactg accaccaaaa
     7561 atattcctgg tgttggtgtg ataaatggaa ttgccgttag tggtaattta aatacgaccg
     7621 acatgtcgac tgatgcagga tttccctatt ccgaacgtcc catcagtttg acgggaaaat
     7681 ggcagtatat gggttttggt gctgatatgg gaattgttgc agtatacctc acgaaatgga
     7741 atagtttaac tactcaacgc gatactgtgg gcgggatttt tcatcctctt acaggtatgg
     7801 aaatgagttg ggcttcgttt agtttgccaa ttgtatacct aagcaacgaa acacccgata
     7861 gttgtttgat tgcacttgct tcaagtggtg aaaacccagt agctaacagc tatttatatg
     7921 tggatgaatt agcttttgaa ttaaactcat ccggattcaa tacagttcaa tcttccgact
     7981 tcattttgta tcccaatcca gcacaaacag aacttacact agaacttcct gcatcatcag
     8041 ctcacatccg attttttgat ttcagtggga agcttatcca gcaacaaaca acaaatggta
     8101 attcccgaca aacaataaac ctgatagatt ttgctccggg tgtttattgg gttcaagtcc
     8161 aaacaaagca aggaatttcg acttctcgat tcatccgtca ataaatcgaa tagagatttt
     8221 caatgcaaat ctagggttga tacattcagc ccttttttat ttctcctgaa ttcgaatcag
     8281 tacattagct tcaaatccca actatgtttc tgcgtcgagt cctcattcca atattgttgt
     8341 tgattgttca ttcatctttg gcagttaaca ctccagaacg agttgagcca gcaaattggt
     8401 ggattggaat gaaaagtcag tttttgcagg ttttggtgaa agcgtcacgt gtcgctcaaa
     8461 tggatgtgca attcaactat ccaggtttgc gtttgcttga aattcatcct ggagatcatc
     8521 ctgattattt gtttctcgat ttcgaagttt cggatcaaat gcaagcaggc gattgtgagt
     8581 tgaactttta caatcctcaa atcaaaggga aaaaacaata tcaatacacc tatcgttttc
     8641 taaaaaggga agaaggctca gctaatcgaa aaggattttc agggaaagat gtgatttacc
     8701 tcatttgtcc cgatcgtttt gtgaatggcg atccaagcaa cgattcgaaa cctgatatgg
     8761 ccgataaact caatcggaat gatccatcgg ggcgacatgg tggcgacata caaggtatca
     8821 ttcaatcgct cagttatttg caacagttag gtattacatc tttatggatt aatccgctgg
     8881 tagagaataa catgcctcaa cattcctatc atgggtatgc gattacagat tactacaaaa
     8941 ttgattcacg gtatggtgat ttgacgcttt acttgaaact tgttcaggaa gctaaattac
     9001 gcggtatgac tatcattgcc gatcaagttg cgaatcattg tggttcttca cattggtgga
     9061 tgaaagcttt accatttaag gattggatta attatcccga ttcggctcag tttaccaatc
     9121 acatgcgaag cactttgcaa gatccacatg ctgtagctgc cgatcgtact ttattttccg
     9181 acggatggtt tgtacctacc atgcccgatt taaatcaacg aaattcttat gtagctaact
     9241 acctcataca aaacagtatt tggtgggtag aaacagccgg attaggcgga attcgaatgg
     9301 atacgtattc ctactctgat aaggcattta tgaatttgtg gtcgaaacgc ttgatggatg
     9361 aatatcctaa cttttcaata gtaggagagg agtggtcgga gaatcctgct attgtagctc
     9421 aatggcaagc aaaagaccta atcgaaccgg gtaaatcggc attgccttcg ttgatggatt
     9481 ttccgctaca agctgcattg gtcaaagcac tcacacaacc cgaaaactgg aattcaggct
     9541 ggattttact gtatgaggca ttagccaacg atttcttgta tccacatcct gagaagctgg
     9601 tcctatttgg agataaccat gatatggatc gtttttattc tcaacttgga gcgaatccag
     9661 cattatttcg tttgggaatg gtgtatttac tcactactct gcgaattcct caaatcatgt
     9721 acggtaccga aattctcatg caaaatgaaa aacataacaa tcatggagaa attcgaagtg
     9781 atttccctgg cggatggcga ggcgattcgg tgaatgtttt tactaatcaa ggactatcga
     9841 cagaacaaat tcaagcacaa caatttatca ttcgtctaac atcatggcgt aaagcttcag
     9901 aagtgattca ttcgggctcg atggtacatt ttgctcccaa aaatggggta tatgtgtatg
     9961 cccgcgaaaa aggaaacaat tccgttttgg tattgctaaa caaagagaat actccaataa
    10021 ccattaatct caagcgattt tcttctatta ttcgagatcg aaaaattgcc tgggatgttt
    10081 tgaaagatca agaaatgacg gttggtgacg agttgatatt atctcccgtt tcagctaaaa
    10141 ttattgaatt ctgaagctca ttttcccttc atttgtgcga gaatttcatc ccttatgatt
    10201 cgtttagcta ttgttttttg tttgtgtact atgacacttt cggcgcaaaa gaaagtcccc
    10261 ggaaatggcc gtatacagcg gtttcctcat tttccatcta atcaggttga accacgaaat
    10321 attgatgttt gggtacctga agattatccg caaaagggac cgtattcggt tatttatatg
    10381 cacgacggac agaatctttt cgattctaca acagcctatg gaaacatacc ttggaaggct
    10441 ggagaagccg catcagcctt gcttcgtgac aagaaaatag cacaatgcat cattgttgga
    10501 attcactcta acgataagcg ttttttggaa tacaatcccc aatcgatttt caaaacactt
    10561 ccagctacac ttcaacaaaa acttgtggct gaatatcaag gcgaacctat ttcagatgca
    10621 tacttattat tcattgtcaa ggagttgaag ccttttatcg acaatacgtt ttacacttct
    10681 cgtgtacgtg aaaatacttg tattgctgga tcgagtatgg gaggattgat ttcactttat
    10741 gcgatgtgcg agtatccgaa agtttttggc tcggcagctt gtatttcgac tcattggccg
    10801 gtaagtctga aaatgaatga tgctcgaata ccgaaagcta ttaaagcata tctaaataag
    10861 aagttgccac cttctactga acatcgcgtt tacttcgatc atggaacaga aaccttagat
    10921 aagcaatatg caccttatca aaaggaaata gatgccctaa tgaaagagaa aggatattct
    10981 aagaagaatt ggttgacaaa ggtatattct ggcgcagctc attctgagct atactggcag
    11041 aagcgcttcc ctgctgtttt gaaattccta cttcctagat aatcaaggtt cggttttaat
    11101 ggtaaacgtt gaataggtta ccatattagg aagcaaaatg gttttatctt ccggataacg
    11161 aagtgtaact atcgtttcaa atggttcaga tagaatcgat tctgttggtt tccatggttt
    11221 attgggcatg attagaagaa taattccatc tgcaccacca acgggtttat aactcgaagt
    11281 agaatttggg aaaacatcta cgccaaattc ttcggatacg atacccaaac atttagtgat
    11341 attctttgta acgactgcta cttctgatat tttcaacaat tgatcttgat taaaatcacc
    11401 tgttacattg gtgccaaaat catgacgagc tatcaattcg atgatgttgc ccgttccatc
    11461 gttaatatag attgaatgag aattgtaaat tggattcgaa cttatttcag ctcccgtaat
    11521 ttcatcggta ataatttcaa ctggattttt agggccatta gcatatttac catcggtgtt
    11581 ttttaaccaa tcaagagctt tttcaatttg atttcctggg attgaaattg caaagtgatt
    11641 aactggagga agagtcgtat tagataagcg atacgttaat cgtgatgagc ctattttaac
    11701 tgtaaaacta acagtattcg tatcaatgat tggcattcct aatgtctcag accaaaaggt
    11761 aaaggcttta cccatatctg aagtccggat ttctaactca gcaatgtcga ccgatttaga
    11821 aggatacgta attggatcta attcttcttt tctgcaagag ttcaacaata ctaataggag
    11881 gaatgcagtt gcaataccag aaagcctact agtcaaatgc ccaaatatgt tatactgctt
    11941 gctcatttac gttgtacctt ttttttcgca attgcagctt tgcgtttttg tgatttggtc
    12001 agttttttct ttttaaccgg agtgaaatat tccggaacgg tatcatcctg actatagtca
    12061 cggtctaagg cgaagcttaa tccaacacga attaccggag atgcctccca aacttcacct
    12121 tctaaataac cacgtgtttt atagcctgct tggaaagcga gtgacattgg gtgattccac
    12181 gatttggtaa gcaataagtt accacttaca atagcagctc cgccccaatt aataggttga
    12241 acttgttgtg ttaaccaatc ttggaagaaa taacgttgtt tccaaatact cacatgcgca
    12301 ttcagggtga agttttggct ccgtttgatg ttgaataatt taattccacc accatacgat
    12361 tctgcgaaat tccctgttcc aattcgagca tatgcttgaa gcatttgtcg gtgattggtt
    12421 agcgtaagtg aaggaatcca ttctggacca aatggagtga agcccaaacg tgcccatgga
    12481 agcacatact ttccataacc taattttatg gcaggagttg ccatagaatc ttctcccgta
    12541 atcatatact tatagaatac actgtataat gcagtataaa cattcgggtt tgtgaaagtt
    12601 gctagtaaat aatcacgttt caaatcagct gctttgtagc gatattccga atagaaacca
    12661 tattgacgat tggtgtaata caaccaattt gcagcagcag tatttccaaa ttctgggtta
    12721 tcacttagcg acataaccga acctaatcct gcaaggtttg ctctaagcaa atgaagtgat
    12781 tctcggtaat ttactgattt gcgttgcatc cagcgaagtg aaagttgatc acttgccatt
    12841 gatcctgcat cgagagcagc acccgatagt tgagccagtt gcatgcgtga ggcttcattt
    12901 tcgagcgttg tttgatcgta ttttattcca ccggaaggtt tccacaatgc gagtggataa
    12961 ggaggcgcga atgtatagtt gatgcctttt agttgaaatt ctcgacttcg tcccatcgag
    13021 ccaaagaaat cttcttcagc agaaaggagt aacgattcaa ttggagcatc gagtaggaat
    13081 atttttccaa aacgatatac taccgaccaa gcacgttgag aggctgttgt atcacctttc
    13141 caacgaatag gaagtaatga atcttcccaa gaggtaagtt gtttttgtaa tgttaaaaca
    13201 gaaggaacac cagcagatgc agttactggc caatccaata aaacattata ctgaattcga
    13261 tcggaatagc gaatcttgaa ttttctaatt tttcgaggtg gacgaggtgg acgaagaacg
    13321 gcaccatctt ttaacaccct ttgtttggga tctttgaata aggaatcacg ccagaattcg
    13381 gttgtatcca ctcgcatggt gtcctttctt ggcttgattg tatcacgaaa tgcaggttga
    13441 ggtttggagg gaacctgagc ctgagccaat gccgggataa ataatataaa taggaggaaa
    13501 attctacgca tgcttttttc tgggcggtta caaaactatc caaactcagc gaaattcaaa
    13561 caattactgc aagggagttt ctaacataca gataatcaat taatttaata gatgtgcttt
    13621 aaagcctctt ttttgctaag tgctgataat gggttactta gcacaaagtc acgaacggct
    13681 tctgcatcgg tttttgctaa ttctcgaagc gaccaaccaa tcgctttgcg tataaaaaac
    13741 tctttttcat gcattaaatt ttgaatcaag tcgaattgaa gctcccaatt tgactctttt
    13801 ttatatttca attgaaccaa taaactagct ctttgaatcc agaagttttt atccttattc
    13861 caccttttac tgattggaat taagtaggaa ggatatttct taaaatatgc ggctgctaaa
    13921 acggggctta agtaatcgat cgaatcccac caagaatttt tacgcataag tgattctaaa
    13981 aaatcgatat cagttaaccg aaagtgtttg cgttgatgaa acaagcattc catggcgaaa
    14041 tagtggtatt ctcgaaactg actttcccag agtaaaggaa ctaattcgtc taacacttct
    14101 aaactaggct tttcatacaa gctaaaaagg tttcgagtag cttctgctct caatggtttt
    14161 tgaatgccta gatacttgaa ttgatatttc atataagccg acatagctac ggctttacta
    14221 ggattagcta ttgctgaaaa tgaatcttgt acaactatga accaatcctg agctcctaat
    14281 gtaactttca tatcggcaat atacagctta gaaataagca ataaaaaagc gcaacgtatt
    14341 gctacattgc gccattattg ggttggatat taacttttta cgtattggat ttcggcagct
    14401 aaacgcacgt cttcactaac caataatcct ccactttcgg taactgcatt ccaagtaaga
    14461 ccaaaatctt tacggttaat tacaccactt actgtgaatc cagcttttag atttccccaa
    14521 ggatcttttg caattccacc caattcggct tttaagctta ttggtaaagt cacttcacga
    14581 atagttaagt taccatgaat agtaaattcc tcttcgttct tcttctcaat ttgggtagat
    14641 tcaaatacca aatgagaata tttttctgta ctaaagaaat catccgattt caaatgtcca
    14701 tctcgttgct catttcctgt attgattgaa tcaacagctg ctttgaaaga aattttagca
    14761 gttgaaaaat cattgtcctc agtagtaact tctgattcca atacaccgaa atgtccggtt
    14821 accgttgtaa tcattaagtg ttttactttg aattgcaatt cactatgtgt aggatctaaa
    14881 acccattttg ttgtactcat ttcgaatttg atttttgtta gtattaagca agttgcatgg
    14941 gaacttccat gacaagaatt tcggtttttt ctaaggattg aaattggata gatggagtat
    15001 cccaaacacc cattccgtct cgtcgattta gaacagtatc acccatttgt atacttcctt
    15061 ctaaaacaaa gagatagatt ccatttcctg gtttttttaa tgcgtaggat gatgaaaaag
    15121 tagaatcaaa gctgcccagg taaaaccaag catcctgatg tatccaaacg ccctcatcat
    15181 ccgcattggg cgataaaatt tgttgtaatt gattgtgacg atcagctttt ggaaagctca
    15241 actgatcata acgtggcttc acatttcgct tattaggaaa tacccaaatt tgaaggaaat
    15301 taacttgttt atcggaatta cgattgtatt ccgaatgggt aattccgcta cctgcactca
    15361 tcacctggac atcgttttcg cgaataacct gcacatttcc catgctatct ttatgctcca
    15421 aatcgcccgc tagcgggatg gaaataattt ccatgttatc gtgaggatgc gttccgaaac
    15481 cttttccttc agctacaata tcatcattca aaacccttag cacaccaaag ttcattcgct
    15541 caggattgta gtattgtgcg aagctaaacg tatgatgcgt attgagccat ccgtgatcgg
    15601 catgaccacg tgtagtggct cggtgtataa tcgtattatt cataatccta tttttttcaa
    15661 gcggtagatg gttgaatcca acttgttcaa ttgctaatga accgactttt tcctcgctct
    15721 gaatcaaacc tgcaatccct agggcacttg ctcccaaaag gcctttcttg aggaaatcga
    15781 tacgcttcat ttagaaaatg tatgtatgta cagcaaatgt acaaacattt aatttcctag
    15841 caagtttatg gtagtgaatt tgatttaaaa gcttgaatta taggtaaata agattcagga
    15901 cggctgttgg ccatcaaact aaaagcgaat ctattttcgg attatttata gcgaatcaac
    15961 aaaacagaac cgtgaaaatg atacggttta ctgtgtttat ctcgggcgtt gaggtgatac
    16021 acatatacgc catcaggtac aagtttacct gaactcattg tgccatccca acttccatcg
    16081 ggtgttgtta tcacataaat ttcttctccc caacgatcat aaatacgcat gttgagttgt
    16141 gtgatttcat aacccgaaat cttaaaaaca tcgttgatat tatccccatt aggcgtaaaa
    16201 ctattgggaa cgtataattc aggttctagt tctattacca caacttgtgt tgctgtgtcg
    16261 gcacatccaa attgattatt acagatgtgc gtaatcgttt gttgagcttc ttctgtaaac
    16321 gtaaacgtat tattatcagc taattcgtag tccaatccat tcaaaaacca agtgcaatga
    16381 tccgcttcat ttcctaaagg cactactacc acatcaattg gtaataaacc agtttcattt
    16441 atctccacat taaacgccgc attaaccgac atatccacta aagttaccgt gtcggttgag
    16501 gtattgcaga aaccctgttg tgttaaaacg taaactcctg cttgaacaac tgaaatagag
    16561 gattgattcg atccatttga ccaactattt ccagttgggg agttggattg aaaggtgatg
    16621 actttaggta agcatgcttt tacaagtgaa ttccctaaaa tttgagcagt aataagtggt
    16681 tcagcaataa agttggttga tgcaggagca ctcacgcaac ctaagttctc tgctgttaca
    16741 aaataggacg catttgaact tgatgtgata ggataagtca agcctgttcc tatttgtgtt
    16801 gtttgtgcag catcggaata ccaagttata attggtgcag tcgatacggc tgtcaatatt
    16861 ggtaaaggag ttcctgcaca ccattgaact tgagcaggta aaacgggtgg ttgaggcgat
    16921 tgattgaggg taatagaaaa actcgaagca ggagattgac aaccagtaac tccctgagtt
    16981 gcataatatg tagctgttgc tgccggaggc gtataagaat ttcctgtacc aaccgatgtc
    17041 gttagagcag catcgctata ccaagttatt gttgaaccag cggcaggtgt tgccgttaat
    17101 ggtgtaattg cagttccact acagaaagta tttgtaccgt taattagtgg agctacaggt
    17161 tgagtaatta ctgtgatatc atgtgtagcc gttgataaac aagcaactgc cccaactgta
    17221 taagttattg cttgtggacc tgttaaacca gctggattaa acagatttcc tgttactcct
    17281 gttccagacc atgttccacc tgccgtacct gtaactagcg tatttaaatc gaggcttgag
    17341 gtagaagtgc aaatcgaagt agctgtccaa gaggcatccg aattagcaat tacattaatg
    17401 gtttgattgc tggtttgaac acacgctcca gtacctactg tgtatgttat tgtttgattt
    17461 cccgataatc ctgttggtga gaatgaagat ccagttactc catttcctgt ccaggttcca
    17521 cccgttgtac ctgtgattaa cgcattgaga ttaatagctg gatcggaagc acaaacagac
    17581 gattgagcaa atgcccaagc cggattagca cttgcaacta cattgatatt ttgtgtgctg
    17641 gtagcttgac aagtacctgt tccaaccgta taagtgatcg agatatttcc acttaatcca
    17701 gctgggtcga atgtatttcc agttacagct gttccagacc aggtgccacc tgaagttcca
    17761 gttagaagtg tattaagatt gacagttcca gcgccgctac aaactgtttg aggagtccat
    17821 gctgcagagc ctccacttac aattgaaatc gtttccgttt ccgaaacggc atcagcacaa
    17881 ttaggtgaaa atgtataagt tatatcaaca tcaccactca aacctgctgg tgaaaagttg
    17941 tgtccagtaa caccgggacc agaccaatat cctcccggag ttccggttac caaagtattt
    18001 aaatcgatag ttgcagctcc agaacacata ggtgctggtg gagtccaact tggatcaggt
    18061 acaatgtaag gagcagtaca accattatta ccgtacgaac aaactcccgc ttgcgtatat
    18121 tct
//
LOCUS       DAOOQW010000030        17857 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_3837_length_17857_cov_5.516234, whole genome shotgun sequence.
ACCESSION   DAOOQW010000030 DAOOQW010000000
VERSION     DAOOQW010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 17857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17857
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3837_length_17857_cov_5.516234"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="PK281_03370"
     CDS             <1..299
                     /locus_tag="PK281_03370"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOY48413.1"
                     /translation="VEIICDDFFIPNVFAPGNGGDPENDCFRLYGTNCISELNFRIYN
                     RWGELVFETNDPEICWDGTFHGQEQNAGVFVYYLKAKLINEESIEKQGNITLIR"
     gene            310..1350
                     /locus_tag="PK281_03375"
     CDS             310..1350
                     /locus_tag="PK281_03375"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HOY48414.1"
                     /translation="MKNWYLIWIISLLLPSYLLGQDIHFSQFTMAPLIQNPAMAGALN
                     NAEAYLNYKNQWKVVTSPYKTYAASGMARIGKPKKDKNFWAVGLSFFNDQSGDGKLTT
                     TTGSFTLTHQIHLSRYHKLGLGLQTGFGQAKINPADLQWGSQYNPGAGYDPSLSSGEN
                     IANPTFSYFDFSSGILWSFNNTAGLVKTSNTVFNKGCAGFAVYHINQPGYSFVESGDK
                     LNMKYVIHGNYLFSINSSKLALNPGFMFYKQGPAKEIFIGSMVRYNILPESKYSGFYK
                     GAGAYLGAYLRARDAVILSAMLELSQYTIGFSYDINTSNLRPASNGRGGFEVSIRVTG
                     QGPFANKMRSFR"
     gene            complement(1393..1465)
                     /locus_tag="PK281_03380"
     tRNA            complement(1393..1465)
                     /locus_tag="PK281_03380"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1430..1432),aa:Ile,seq:cat)
     gene            complement(1583..2557)
                     /locus_tag="PK281_03385"
     CDS             complement(1583..2557)
                     /locus_tag="PK281_03385"
                     /inference="COORDINATES: protein motif:HMM:NF019365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015926 - glucosidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HOY48415.1"
                     /translation="MRGIDISSLPEIETGNTSFFSSLGIKTDLLSILKDRGVNTIRIR
                     LWKDPVKEHSGFEEVKLFSQRVHQMGMKVWLTVHYSDTWADPGHQQMPVVWSNVSYSN
                     LKDSLNHYTTKIMQEIQPEFIQIGNEINPGMLLPAGEIVSRKDQLVDLLNTCTQAVRA
                     FSNQTRIVIHFAGLENASWFFNQISEVDYDVMGLSYYPIWHGKDLDVLQNTLNTLVQT
                     YSKDIVVAETAYPFTLGWNDWTNNVVGGDDQLILPDYPASPEGQKQFVNRIRSIVENT
                     PRGIGFIYWGGELVSTQGPESTQGSTWENQAVFDFTNKELPVLEAFHK"
     gene            2463..2678
                     /locus_tag="PK281_03390"
     CDS             2463..2678
                     /locus_tag="PK281_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48416.1"
                     /translation="MESKSVFIPRDEKKDVFPVSISGKDEISIPRINESDSATTSIGW
                     LLVELASSVLEQENNKSKSEKWKINLD"
     gene            2795..3427
                     /locus_tag="PK281_03395"
     CDS             2795..3427
                     /locus_tag="PK281_03395"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOY48417.1"
                     /translation="MIFEGQHIDAIIFDLGGVILNIDYQLPIKAFQKLGIDDFANHFS
                     QAKQSGLLDDYETGSISSEDFIAELRRYVKPETTDIQIISAWNSILLDLPEERLFILE
                     KAAENHRTFLLSNTNDIHIEEFNSYLLEEHNLPSLEPFFEQLYLSYEVGLRKPDPRIF
                     EYVLQDAELNPSSTLFIDDSIQHIQAAKELGIITHHLTGQTLVELLEDKL"
     gene            3439..4026
                     /locus_tag="PK281_03400"
     CDS             3439..4026
                     /locus_tag="PK281_03400"
                     /inference="COORDINATES: protein motif:HMM:NF024769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HOY48418.1"
                     /translation="MELKNGIATFYAASRQDWRNWLEKNAENEISVWLIIHKKASPHP
                     SIYYPEAVDEALCFGWIDSKANTRDEFTYYQFFSKRKPKSNWSKVNKIKVAQLIEQGL
                     MRPSGLKMIEHAKSIGTWDALNEVEEIRVPDEMQKLFDQQPIAFQHWNLFSRSVKRGI
                     LEWILNAKRPETRTKRIVETVSLAAENKKANQYRQ"
     gene            4108..5793
                     /gene="ettA"
                     /locus_tag="PK281_03405"
     CDS             4108..5793
                     /gene="ettA"
                     /locus_tag="PK281_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013552135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HOY48419.1"
                     /translation="MSDDKKVIFSMVKVSKTFPPQKQVLKDIYLSFFYGAKIGIIGLN
                     GSGKSTLLKIIAGVEKSFQGDVVFSPGYRVGYLEQDPQLDDSKTVIEIVREGVQPIMD
                     ILAEYNEVNEKMGLEEYYMDGDKMDKLLARQGVLQDEIDSKNAWEIDSTLERAMDALR
                     CPDSDRIIGTLSGGERRRVALCRLLLQQPEILLLDEPTNHLDAESIDWLEQHLQNYAG
                     TVIAITHDRYFLDNVAGWILELDRGEGIPWKGNYSSWLEQKGKRLQQEEKTESKRQKT
                     LARELEWVRQGAKGRQTKQKARLQAYDKLMSEDVREKEEKLEIFIPNGPRLGNEVIEA
                     QDVSKAFGDKLLYEHLNFRLPPAGIVGIIGPNGAGKTTIFKMIMGQEKADVGEFKIGP
                     TVKISYVDQTHESLDPNKSVWEVITGGNETMEIGGRSMNSRAYVSRFNFNGADQGKKV
                     GVLSGGERNRLHLAMALKAEGNVLLLDEPTNDIDVNTLRALEEGLENFAGCAVIISHD
                     RWFLDRVCTHILAFEGDSQVYVFEGGYSEYEENKKKRLGDHEPKRIRYKKLVN"
     gene            5887..6153
                     /locus_tag="PK281_03410"
     CDS             5887..6153
                     /locus_tag="PK281_03410"
                     /inference="COORDINATES: protein motif:HMM:NF039417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6364 family protein"
                     /protein_id="HOY48420.1"
                     /translation="MDSKITLSFNSSVIDKAKAYADEQGISLSRLTEILLRRLTSGGY
                     KNIEDFPVSDWVHLISEGETEYIRKPKSSKALRAEHHTAKKKKA"
     gene            6150..6566
                     /locus_tag="PK281_03415"
     CDS             6150..6566
                     /locus_tag="PK281_03415"
                     /inference="COORDINATES: protein motif:HMM:NF024861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HOY48421.1"
                     /translation="MKVFVDANVIINVLNREYPAFEFSSRLLSLADDKRFTLYTSTLS
                     LGICWYHAEKKSGRALANKKIRQLLQHIRITDCGESEVLKAIEDKKAEDFEDALQVYS
                     AIKSGCSHIVTNNLNDFHFCVLPISNPRDFLIEQAS"
     gene            6801..7271
                     /locus_tag="PK281_03420"
     CDS             6801..7271
                     /locus_tag="PK281_03420"
                     /inference="COORDINATES: protein motif:HMM:NF015060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HOY48422.1"
                     /translation="MLNRFLRLLLCISIPLIIGGISGYFTIGGVKSWYLTLNKPSFTP
                     PNWIFGPVWTTLYALMGYAYFRILEKAKSPAQLFAIRIYFIQLFLNFCWSILFFSAHQ
                     IGIALIDILLLWICILIMIVRFYRVDKFAGYSQIPYLLWVSFATALTFSIWWLN"
     gene            complement(7334..9391)
                     /locus_tag="PK281_03425"
     CDS             complement(7334..9391)
                     /locus_tag="PK281_03425"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY48423.1"
                     /translation="MRQKLLLGFLFFISSFVIHGQIPYGVYEVPFHPLSPDQATSIQL
                     YDDMYSAPIPIGFNFRYFGTDYSSLNIASNGYVTFNPELEGAYSNFSVSTHIPDTTAP
                     LNSIFFTWCDINPMNAGNISYGIIGDAPNRIFVVQFIQVPLFACTQTYTGQLQLFEGS
                     NNIEVHITSKLFCDTQGGLSSIAIEGIQNATGTEAYAVPGRNNYGVWQASNDAWRFDP
                     DTVSQPVCIMSGRVIADFNGNCILDESDFSIPGQVLIRDNGEAYTSTTGQGVYSFEAD
                     TGNYTIAFNGLQSGLPFAEISCPTDASYNVSFETAGSVSNMLDFYVHPDSSCSDIRVS
                     LVPMGPMLRCAGNTNHQYVSVSNHGLLPIIGYTVSLTLPDSMSVMETIPAFSSQVGNT
                     LTWNFTDTLVYGEYTQISISDSLSCYATDGTLKCLSATVQSDADCNTGNNSSEVCQIV
                     NGSFDPNHIQLLEMSSPAEFVNELLVAGNETWYTYRIEFQNTGTGPAQTVVITDVLPA
                     FFDYGTAEILATSHSAFLVNLGNGTLKFVYNGISLPDSNTNYAGSIGSVVFRVKANQM
                     LFPGQQVANQASIVFDVNEPVITNQALIRVPNVTGISSIENSVSMYPNPANQSIRIKS
                     SNQLVDRVSILDLSGKQVGNYPISKAEETLVIDQLANGIYLVEFYQNQRKITQKKLIV
                     SHQ"
     gene            complement(9501..11498)
                     /locus_tag="PK281_03430"
     CDS             complement(9501..11498)
                     /locus_tag="PK281_03430"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY48424.1"
                     /translation="MGISQSIYEVYSIPFSPLVPDQTIGIPLLDDNYSNSISIGFPFK
                     FFGNSYTTLRVSANGFVSFNSGSLSTNYIISDTIPSTAVPSNSILITYFDLNPVAGGE
                     INYQTIGEAPNRIFVLSYNQVAIFGSNCINTLYTGQLQLYENKSTIEIHITSKEICNS
                     WTNRALEGIQNSTSTIGYSVPGRNTINPWEAFNDAWRFEIDTTIYSISGRVIADINGN
                     CFLDSSDYTIPGQVIILNNGLDYTATNNQGSYSFEVLNSNYQLKFNTLQENLPIADVV
                     CPTDAFYTVQVNTPDSVYNLYDFYISPDTFCIDPRVFISPVGNLQSCVGNENHQLISV
                     ENYGFEPMVGYTVSCTIPNSVTIINTIPEYTSQNGQTYTWSFTDTLLYGEFTSIHIFD
                     SLACDLVDSSLICFHVNAESIHDCDPSNSQADICQYSNNLFIPNHIQLLDTLPIIQFV
                     DNLIEYTTDTWYTYRIEFQNTSPDSTHRIIIRDEIISFFDRNSVELLSSSHPCKLINL
                     GNGSVLFDFDDINLPNNLQDFYSSIGNLVFRVKSNQVLLPGASVSNYATIKLNDISSI
                     QTNSATIHKPNILGIPDLNSEFSIYPNPTADFINIIDKSSQINRIQIFDIEGKKIQES
                     SISKSKSQIQVSHLRAGIYLIKLFSINGEIYNQKLIIVNPN"
     gene            11670..12086
                     /locus_tag="PK281_03435"
     CDS             11670..12086
                     /locus_tag="PK281_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase"
                     /protein_id="HOY48425.1"
                     /translation="MNLLQKRIHELNEIERSAQESAAGDTKSSMGDKHETSRELLQQE
                     REMAGRRMAEAIRQKEELERILPEHVLNQVQTGALVETSIGTFFFATALGFITLHEKK
                     IAVLSIASPIGQALKEKQLGDIVSFQNKDIRIISIA"
     gene            12242..13216
                     /locus_tag="PK281_03440"
     CDS             12242..13216
                     /locus_tag="PK281_03440"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY48426.1"
                     /translation="MKAILFTLGFSLFANFIVAQVPKKIIVEHFTNSRCSICANRNPG
                     FFDNLSQHPDVLHISMYPSSPYPNCYFSQQNANENDGRTNYYQVYGSTPVFVIQGEVI
                     NQSSNYSSAALFEPYLNQTSPISIQVNQIQSSDSIISTISVKTEAENSLGELVIFGAI
                     AEDTVFFSSPNGETEHYNVHRKSLYGDSGIPISIPSTIGDSVTLRKAIAKNSNWNYTR
                     LKSVIIIQESSTKTVVQAAESDTNNGITTGQINLTCNKIDIYPNPVESFLTIKGYGTN
                     MQNYYLINSTGIIQKQGIMNQETKLDFTELPHGIYLLKIGTEIHKIIH"
     assembly_gap    13367..13466
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(13701..15539)
                     /locus_tag="PK281_03445"
     CDS             complement(13701..15539)
                     /locus_tag="PK281_03445"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY48427.1"
                     /translation="MMTRISRILAILIITIGGLNAQTDHTTQVQTQTRNSEVNTHSPL
                     GTLKHNNNEGNNQMITICHQTGNGGSHTITINYNALQAHLNHGDHIGTCTSGNDDDDN
                     DDEGEDNDDNGDDDNDDEGENNDDNGDDDNDDEGDDNNDNGDDDNDDEGDDNNDNGDD
                     DDDETTDGGDTTVVEPSPAGCNAVQVMSYIPGTTNDLLTPIEAARQNLINALGSPEDS
                     DVATPSTAYNFVALGFGGEIILKFANPIHNGDGDDIYVVETTFGNNSQNCSRYPEKIR
                     AFASQNNCDWVFLGEGCQNTYFDLQGLAWAQYVKLIDISRIDFPYGGLADGYDLDGIV
                     CLNGEEVNPVLSTVSNEFAVEAYDYLPGTMKNGGAVPAARSNKDNAVGAPQYSDVVNF
                     VSLGFGGKVSLKLGYVVFNKPGADFMLIETSYGNPSCDNYPETARIEVSLDNTTWYML
                     SESYCLDQSIDIEGSGTTMFQYVRISDASAMSNSHFPGTADGFDVDGIVVAQPGCSST
                     STARIAQVQSQDNTTTANEVAEISVYPNPFNETLSVNLSTSNTGETFEVSIINTLGQV
                     MIRENVEVASSSTIRHMLPTSDLAKGTYLISVRSANSNEVIRTVKF"
     gene            complement(15910..16671)
                     /locus_tag="PK281_03450"
     CDS             complement(15910..16671)
                     /locus_tag="PK281_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014083824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HOY48428.1"
                     /translation="MKHLGIINQFRYFILLVWIGLLVSVLGSFGAPPIVIDNSDSRII
                     SLEINPTKQDVQFYWKDAAGNKLKSIQQLKTFVESRGEVLQFAMNGGMYTEDNSPLGL
                     YIEEYKTLTSLNTKSGNGNFYLKPNGVFYLLKNRKAVVCRTEEFKINPNIRYATQSGP
                     MLVIDGKIHPEFKLGSSHVNIRNGVGILPNGNILFAISKEEINLYDFAQYFKQAGCNN
                     ALYLDGFVSRMYCPEKKQYSLDGNFGVMIGVGIRK"
     gene            complement(16684..17070)
                     /locus_tag="PK281_03455"
     CDS             complement(16684..17070)
                     /locus_tag="PK281_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013621108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOY48429.1"
                     /translation="MNLNQVTLPVLNVKQAIEFYTKLGLELIVQSIPEYARFVCPDNG
                     STLSVHRVDTLPQGDGIWVYFELENLDEYVGKIQSQGIKLDEMPTDKPWLWREARLKD
                     LDGNQLIFYTAGGNRINPPWRVGNTK"
     gene            complement(17073..>17857)
                     /locus_tag="PK281_03460"
     CDS             complement(17073..>17857)
                     /locus_tag="PK281_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48430.1"
                     /translation="DFMKHPDKRPIQNYLLGSYPVKIQELYEVQAVGQMYKYNFDLAI
                     KSFAKDSTSGLTELLGNPFNMGITDCHDCDHQKFQKVKYNKRTFASKMLEMKNKTMSS
                     KEAQEKANNAFLYANGLYNMTWYGNARLVSSTVVNWDYIDTYHLNSYDNEPRKKSPYY
                     DCAEAQRWYLKAMAWSTNKEFKAKCAWMAAKCEHNLWLEGEYNLMNTEGEEEQGDFKS
                     GIYFNLMKASFSDTQYYKDVIQECGYFCTYLNPEDKSCIKNK"
BASE COUNT         5720 a         3346 c         3139 g         5552 t
ORIGIN      
        1 cggtcgaaat aatttgtgat gatttcttca ttcccaatgt atttgcacct ggcaatggag
       61 gagatcccga gaatgattgc ttccgtttgt atggtaccaa ttgtatttcg gagttgaact
      121 tcagaatata caaccgatgg ggagaattag tttttgaaac caatgatcca gaaatatgct
      181 gggatgggac attccacgga caagagcaaa atgctggagt atttgtctat tacctcaaag
      241 cgaagctgat aaatgaggaa tcgattgaga aacaaggaaa tattacacta atccgataaa
      301 taactgaaaa tgaaaaactg gtatttaata tggattataa gtttgcttct cccgagttac
      361 cttcttgggc aggatataca tttctcacag tttacaatgg ctcccttgat tcagaatcca
      421 gcaatggcag gggcgttaaa taatgctgaa gcttatttga attataaaaa tcagtggaaa
      481 gttgttacaa gtccttataa aacctacgca gcttcaggta tggcgcgaat tggaaaacca
      541 aaaaaggata aaaacttttg ggctgttggc ttaagctttt ttaatgatca aagtggtgat
      601 gggaaattga caacaacaac aggatcattt actttaacac atcaaatcca ccttagtcgt
      661 tatcataaat taggattggg tttgcaaact ggttttggtc aagctaaaat aaatccggcc
      721 gatttacaat ggggaagcca gtataatcca ggtgcagggt atgatcctag tttatcgagt
      781 ggagaaaata tcgctaatcc aacattctcg tattttgact ttagcagcgg cattttatgg
      841 tcgtttaata atactgcggg cttggttaaa acatcgaata ctgtatttaa taaaggatgt
      901 gctggatttg ccgtctatca catcaatcaa ccgggttatt cttttgttga atcgggggat
      961 aaattaaata tgaaatatgt aattcacgga aattacctat ttagcataaa tagctcaaaa
     1021 ctggcgctaa atccgggttt catgttctat aaacaaggtc cggcaaaaga aatttttatt
     1081 ggttcaatgg ttcgatataa cattctacct gaatcaaaat attctggatt ttataaaggc
     1141 gcaggagctt atctaggtgc gtatttacgt gctcgtgatg ctgttattct ctcagctatg
     1201 ctcgaacttt ctcaatatac tattggattt agctacgata tcaatacttc taatcttcgc
     1261 cctgctagta atggacgtgg tggatttgaa gtatccatcc gtgttaccgg tcaaggtccg
     1321 tttgctaata aaatgagaag tttcagataa taaaaaacgg acaagctgat ttaacaacat
     1381 tgtccgtttt tgtgggcctg gcaggaatcg aacctgcgac ccgcggatta tgagtccgat
     1441 gctctaaccc ctgagctaca agcccgtttt cagggacggc aaatatagac tgtcagcgct
     1501 taattccaaa atcaaaggct gtattcttct atttaattaa aatccactgg tagcaagaga
     1561 ttgacagata caaaatgatc tatcatttat ggaaagcttc taatactggt aattctttat
     1621 tcgtaaaatc aaaaactgct tgattttccc aagttgaacc ttgagttgac tccggacctt
     1681 gtgtcgaaac taattcacca ccccaatata taaatccaat tcctctaggt gtattttcta
     1741 ctatagaacg aattcgattt acaaattgct tttgaccttc tggactagca ggataatctg
     1801 gtaaaatcag ttgatcgtct ccaccaacaa cgttgttagt ccaatcattc caacctaacg
     1861 taaatggata agcagtttcg gcaacgacaa tgtctttaga gtaggtttga acaagtgtgt
     1921 ttaaggtgtt ttgcaacaca tctaaatctt ttccatgcca aatgggataa taggataagc
     1981 ccataacgtc ataatcaact tcactaattt gattgaaaaa ccagctggca ttttccaaac
     2041 cagcaaaatg tatcacgatg cgcgtttgat ttgaaaacgc tcgaacagcc tgtgtgcaag
     2101 tattaagaag gtcgacaagc tgatcttttc tggatacaat ttcacctgca ggaagaagca
     2161 ttccgggatt tatttcattt cctatttgaa tgaattccgg ctgaatttcc tgcattattt
     2221 tggtggtata atgattcaaa ctatccttaa gattagaata agaaacattt gaccaaacaa
     2281 ctggcatttg ttggtgtcct ggatcagccc aagtatcaga ataatgaacg gtaagccaaa
     2341 ctttcatccc catttgatga acacgttgag aaaataattt cacttcctca aaacctgaat
     2401 gttccttcac cggatctttc caaagtcgaa tgcgtatagt attcacacct cgatctttca
     2461 atatggaaag taaatctgtc tttattccaa gggatgaaaa aaaggatgta tttccagttt
     2521 caatttcagg taaagatgaa atatcaattc cccgaataaa tgaatctgac tcagctacaa
     2581 catcgattgg atggctttta gttgaacttg ctagctccgt tttagaacag gaaaacaata
     2641 aaagtaaaag cgaaaaatgg aagattaatt tagactgatt gatcattttt catgaaccaa
     2701 ttctttcgaa attgattttc aaatctagtt tattcgaggg agattaatct aaagtttagc
     2761 aagtattcga acggatagat tttacatttg caccatgatt ttcgaaggac aacatatcga
     2821 cgccattatt ttcgacctag gaggtgtaat tctcaatatt gattatcaac tccctattaa
     2881 agcatttcaa aaactgggaa ttgacgattt tgccaatcat ttttctcaag ctaaacaaag
     2941 tggtttgctt gatgactatg aaactgggtc gataagttct gaagatttta ttgcagaatt
     3001 acgtcgatac gtaaaacccg aaacaaccga tattcaaatc atttctgctt ggaactccat
     3061 ccttcttgat ttacccgaag agcgtttatt tatcctcgaa aaagcagccg aaaatcatcg
     3121 cacatttttg ctgtcaaata ccaacgacat tcacatcgaa gaatttaatt cctacctact
     3181 cgaggaacac aatctgcctt cgcttgaacc attcttcgaa caactctatc tttcatacga
     3241 agttggtttg cgtaaacccg atccgcgtat tttcgaatat gtgctacaag acgctgaatt
     3301 gaacccttct tctaccctat tcatcgacga ttctattcaa catatccaag ctgctaaaga
     3361 acttggcatc atcacgcatc atttaaccgg acaaactctg gttgaattat tagaggataa
     3421 actctgagta attcatttat ggaattaaaa aacggcatcg ctacatttta cgcagcttct
     3481 cgccaagatt ggagaaactg gttagagaaa aatgccgaaa acgaaatttc tgtttggctc
     3541 atcatccata aaaaagctag tccgcatcca agtatatatt atcccgaagc tgtcgatgaa
     3601 gcactctgtt ttggctggat agatagtaaa gccaatacgc gagatgaatt cacctattac
     3661 caatttttct ccaaacgaaa acctaaaagc aattggagta aagtcaataa aataaaagtg
     3721 gctcaattaa ttgaacaagg attgatgcgt ccttcgggtt taaagatgat tgaacatgct
     3781 aaatcaattg gtacttggga tgctttaaat gaggtggaag aaattcgggt gccggatgaa
     3841 atgcagaaat tattcgacca acaacctata gcctttcaac attggaattt attttcacgc
     3901 tcagtaaaac gaggtatctt agaatggata ctaaacgcca aacgtcccga aacacgaacc
     3961 aaacgaattg ttgaaactgt ttcattggca gcagaaaata aaaaggcaaa tcaatatcga
     4021 cagtagtttc tattttcaca gatcaatcta aggaaatcac cttaataaac gtgcattttc
     4081 ctatctttgc gctccttttt acatactatg tcagacgata aaaaagtcat tttttccatg
     4141 gtgaaggtca gcaagacctt tcctcctcaa aaacaagtac tgaaagacat ttatctgagc
     4201 tttttctatg gtgccaaaat cggtatcatc ggtttaaatg gttcaggtaa atcaaccctt
     4261 ttgaaaatca ttgctggagt ggagaaatcc ttccaaggtg atgtggtttt ctctccaggt
     4321 taccgtgttg gttatttgga gcaagatcct caattggacg acagtaaaac ggttatcgaa
     4381 atagttcgtg aaggtgttca acccatcatg gatattttag ccgaatacaa cgaggtgaat
     4441 gaaaaaatgg gccttgaaga atattacatg gatggcgata agatggacaa gcttcttgcc
     4501 cgtcaaggcg tgctacagga cgaaattgat tccaagaatg cctgggaaat tgattctact
     4561 ttggagcgcg ctatggatgc cttgcgttgt ccagattctg accgaattat cggaacactt
     4621 tcgggtggag agcgtcgccg agtagcactt tgccgactat tacttcaaca acccgaaatt
     4681 ctcttgctcg atgagcctac caaccacttg gatgccgagt cgattgactg gttagagcaa
     4741 cacttacaga actatgcggg tactgttatc gctattaccc acgatagata tttcctagat
     4801 aatgttgctg gttggattct cgaattagat cgcggtgaag gaattccttg gaaaggaaat
     4861 tactctagtt ggctagaaca aaaaggaaaa cgacttcaac aagaagaaaa aaccgaatca
     4921 aaacgtcaga aaacgttggc tcgtgagctt gaatgggttc gtcaaggtgc taaaggccgt
     4981 caaacgaaac aaaaagctcg tctacaggca tacgataagt tgatgagtga agatgtacgt
     5041 gagaaagaag agaaactcga aatctttatc ccgaacggtc cacgtttagg gaatgaggtt
     5101 attgaagcgc aagatgtttc aaaagcattt ggtgataaat tactttatga gcatttgaat
     5161 tttcgtcttc caccagctgg tattgtagga attattggtc ctaacggtgc gggaaaaacg
     5221 accattttca aaatgattat gggacaagaa aaagcagatg taggtgaatt caaaattggt
     5281 ccaacggtta agattagtta cgtggatcaa actcacgaat cactcgatcc gaacaaatcg
     5341 gtttgggaag taattacggg aggaaacgaa acgatggaaa ttggtgggcg ctcgatgaat
     5401 tctcgtgctt atgtgagccg tttcaatttt aacggagctg accaaggaaa aaaagtaggt
     5461 gtattatcgg gtggtgagcg taaccgtttg cacttagcta tggcattaaa agctgaaggc
     5521 aatgtgctat tgctcgatga gccaacgaat gacatcgacg taaatacact tcgtgcctta
     5581 gaagaaggtt tagaaaactt tgctggatgt gcagtaatta ttagtcacga ccgttggttc
     5641 ttagatcgtg tttgtacaca tatattggca ttcgaaggcg attctcaagt gtatgtattt
     5701 gaaggaggat attcggaata cgaagagaat aagaagaagc gacttggtga tcacgaaccg
     5761 aaacgtatcc ggtataagaa actggttaac taaaacacaa aagcacatca ataaatggtg
     5821 tgcttttttt ttaggatttt ggttttacgt atgaaataaa tatttttgtg gtataaacaa
     5881 aatgccatgg actccaaaat aacgctcagc ttcaattcca gtgttattga taaagcaaaa
     5941 gcatacgccg atgaacaagg tattagccta agcaggctca ccgaaatcct tttgcgtagg
     6001 cttaccagtg gcggctataa aaacatcgaa gatttccctg tctcagattg ggttcactta
     6061 atttcagaag gcgaaaccga atacatccgt aaacctaaaa gcagtaaagc acttcgagca
     6121 gagcatcata cagccaaaaa gaaaaaggca tgaaggtgtt tgtggatgca aatgttatca
     6181 ttaatgtact caatcgagaa tatcctgcat ttgaattctc ttctcgttta ttgagtttag
     6241 cagatgataa acgctttacg ctctatacat caactttgtc actaggaatc tgttggtatc
     6301 atgcagagaa aaaatcagga cgtgctttag ccaataagaa aatcagacaa ctgcttcaac
     6361 atattcgtat aactgattgt ggcgaatcgg aggttttaaa agcaattgaa gataaaaaag
     6421 ccgaagattt tgaagatgca ctacaggttt attctgccat taaaagtgga tgttctcaca
     6481 ttgttacaaa taatctgaat gacttccatt tttgtgtatt accgatttcc aatccgagag
     6541 attttttaat tgagcaggcg agttaataaa atccaattaa ttattcaatt atttctgttg
     6601 ggttttaaac tatatcaaaa cccaacataa tagcatattt caagaatatt taatcaaata
     6661 atattctagt tatttattgg aattaattaa aatataaatt attaaacatt catccattat
     6721 tttctgacta aactatttaa acctgtctat tcgaacaata caggttttac atggtttgta
     6781 ttcctaccta ttctaaaact atgcttaatc gttttcttcg tttattgctc tgtatttcaa
     6841 ttccactaat cattggcgga atttctggat attttaccat agggggcgtt aaaagctggt
     6901 atctaacttt aaataaacca tcatttactc cacccaattg gatttttggc cctgtttgga
     6961 caaccttata tgcattgatg ggctatgctt atttccgaat cttagaaaaa gctaaaagcc
     7021 cagctcaact tttcgccatt cgcatatatt tcattcaatt attcttaaat ttttgctgga
     7081 gtattttatt tttcagtgct catcaaattg gaattgcttt aatagatatt cttcttcttt
     7141 ggatatgcat tctaattatg atagtccgtt tctatcgtgt agataaattc gctggctact
     7201 ctcagattcc ctatttactg tgggtgagct ttgctaccgc cctaaccttt tcaatttggt
     7261 ggctgaatta agtaataaaa aaactccttc aaaaatgaat tctgaaggag tttactattt
     7321 gtttatttaa gcattattga tgcgaaacaa ttagtttttt ctgcgttatt tttcgctgat
     7381 tctgatagaa ttctaccaaa taaattccat tcgctaattg gtcaataacc aaggtttctt
     7441 ctgcctttga aatagggtaa ttaccaactt gttttccact taaatcaagg atagaaactc
     7501 gatcaactaa ttgattagac gatttaattc ggattgattg attagctgga ttcggataca
     7561 tagaaacact attctcaatt gacgaaattc cagtaacatt cggaacacga atcaaagctt
     7621 gattagtaat tacaggttca ttcacatcga acacaatgga agcttgatta gcaacttgct
     7681 gtcctggaaa aagcatctga ttagctttta cacgaaacac tacactacca atactacctg
     7741 catagttcgt attactgtcg ggtagagata tcccattgta aacaaatttc aaagttccgt
     7801 ttccaagatt aaccaagaat gctgaatggc ttgtagctaa tatttcagct gttccatagt
     7861 cgaaaaatgc tggtaaaaca tcggttataa ctaccgtttg cgcaggacca gttcctgtat
     7921 tttggaattc tattcggtag gtataccaag tttcatttcc tgcaacgagc aattcattga
     7981 caaactcagc tggagaagac atttctaaaa gctgaatatg atttggatca aatgagccat
     8041 tcacaatctg acaaacttct gaactattgt tacctgtatt acaatcagca tccgattgaa
     8101 cagtcgccga caaacatttc aatgttccat cggtagcata acagctcaaa gaatcactta
     8161 tggaaatttg tgtgtattcg ccgtaaacaa gtgtatcggt aaaattccag gtaagtgtat
     8221 ttcctacttg acttgaaaaa gcgggaattg tttccataac cgacatagaa tcgggtaatg
     8281 ttaaactaac tgtgtagcca attatcggta ataaaccatg attggaaacc gaaacatatt
     8341 ggtgatttgt atttccagca caacgcaaca tcgggcccat tggaaccaaa gaaactcgaa
     8401 tatcggaaca agaagaatca ggatgaacat aaaaatccag catgttactt accgatccag
     8461 ccgtttcaaa actaacatta taagaagcat ccgtaggaca cgaaatttct gcaaaaggta
     8521 atcccgattg taaaccatta aatgcgatgg tgtaatttcc agtgtcggcc tcgaaactat
     8581 acacaccttg cccggttgtt gacgtgtacg cctcaccatt gtcgcgaatt aatacttgac
     8641 cgggaataga aaaatccgac tcatctaaaa tacaattccc gttaaagtca gcaattacgc
     8701 gtcccgacat gatacaaaca ggttgagaaa cggtatctgg atcaaatctc caggcatcat
     8761 tcgaagcttg ccaaacacca taattattac gtccaggaac agcgtacgct tctgtaccag
     8821 tggcattttg aattccttca atggcaatgc tacttaagcc accttgagta tcacaaaata
     8881 atttggaagt aatgtgcact tcaatattat tggaaccttc gaacaactgc aattgtcctg
     8941 tataggtttg tgtgcaggca aataatggaa cttgaataaa ttgaacgaca aatattctgt
     9001 taggtgcatc cccaataatt ccataagaaa tgttcccggc attcatcgga ttaatatcac
     9061 accaagtaaa aaagattgaa ttcaatggtg ctgttgtatc aggaatatgg gttgatactg
     9121 agaaatttga ataagctcct tccaattcag gattaaaggt cacatatcca ttcgatgcaa
     9181 tattcaaact tgagtaatcc gttccaaaat accggaaatt aaatccgata ggaatcggtg
     9241 cagaatacat atcatcataa agttgaatac ttgtagcctg atctgggctc aagggatgaa
     9301 atggcacctc ataaactcca tatggaattt gaccatgaat aacgaaactg cttataaaaa
     9361 acaggaatcc aagaagtaat ttttgtctca tatggaggat tttagatgta taaaaataga
     9421 atttttattg atatacgaac cattttcatg gaaagcacat taattttttt ctatgatttt
     9481 aacaagtctg atttttcctt ttaattcgga tttacaataa ttaatttctg attgtatatt
     9541 tccccattta ttgaaaataa tttaatcaaa taaatacccg ctcttaaatg actaacttga
     9601 atttggcttt tcgattttga aatactactt tcttgaattt ttttaccctc aatatcaaaa
     9661 atctgaatac gatttatttg ggaggattta tcaataatat ttatgaaatc agcagttgga
     9721 ttaggataga ttgaaaattc tgaatttaaa tctggaattc ctaatatatt gggtttatga
     9781 atagttgcgc tattagtctg aatagaacta atatcattta gtttgattgt agcataattt
     9841 gaaaccgaag ctcctggcaa taaaacttga tttgatttca ctctaaatac aagattacct
     9901 atacttgaat aaaagtcctg aagattatta ggtaaattaa tatcatcaaa atcaaaaagt
     9961 actgaaccat ttcccagatt aattaattta caaggatgtg atgaacttaa taattcaacc
    10021 gaattgcgat caaaaaaact aatgatctca tccctaataa ttatacgatg agttgaatcg
    10081 ggactcgtat tttgaaactc aatccgatag gtataccaag tgtctgtagt gtattcgata
    10141 agattatcga caaattgaat tattggtaat gtatctaata attgaatatg gtttggaatg
    10201 aacaagttgt tcgaatattg acatatatct gcctgactat ttgatgggtc acaatcatgt
    10261 atactttcag cattaacatg aaaacaaata agtgaagaat caactagatc acaagctaac
    10321 gaatcgaaaa tatgaattga ggtaaactct ccataaagta aagtatcagt aaaactccaa
    10381 gtatatgttt gaccattctg gctagtatat tcaggaatgg tatttattat tgtaaccgag
    10441 ttaggaattg tacatgaaac ggtataacct accattggtt caaagccata attttcaaca
    10501 gaaattaatt gatgattttc atttcccaca catgattgta aatttccaac aggagaaata
    10561 aatactcttg gatcaataca aaaggtgtcg ggggaaatat aaaaatcgta gagattatat
    10621 acggaatctg gtgtattaac ttggactgta tagaatgcat cagtaggaca aactacatca
    10681 gcaatgggta aattttcctg aagtgtatta aatttaagtt ggtaattact atttagaacc
    10741 tcgaaagaat aagaaccttg attattggta gcagtgtaat ctaaaccatt gttcaaaata
    10801 atgacttgtc ctggaattgt ataatcagaa ctatccaaaa aacagtttcc attaatatca
    10861 gcaataactc tcccagaaat agaataaata gttgtatcta tttcgaatct ccatgcgtca
    10921 ttaaaggctt cccaaggatt aattgtattt ctgccaggta cagaataacc gatagttgag
    10981 gtgctatttt gaattccttc tagtgctcta tttgtccaag aattgcatat ttccttactg
    11041 gtgatatgaa tttcgatagt gcttttattc tcataaagtt gaagctgacc tgtatagagc
    11101 gtgtttatac aatttgagcc aaatattgca acttgattat aggataacac aaaaatgcgg
    11161 ttaggagctt caccaatagt ttgataatta atttctccgc cagctacagg atttaaatcg
    11221 aaataagtaa tcagtataga attactcgga actgcagtac ttggaattgt gtctgatatt
    11281 atataattgg ttgataaaga tccgctgtta aaggatacaa aaccgtttgc tgaaaccctt
    11341 aacgttgtat aagaatttcc aaaaaattta aaggggaatc caatagaaat tgaattggag
    11401 taattatcat ccaacaatgg aataccaatt gtctgatcgg gcaccaatgg agaaaaagga
    11461 atagaataaa cttcgtagat ggattgagaa attcccaaaa aactctcaac taagaataat
    11521 agtgtgaata gatatcttaa atacattagg tttgatttac aattgtgaat ctaatttaaa
    11581 tttccgtatc tacaaatcgg aaccatgaaa tgcaagagct aaattctaat aacaaggcaa
    11641 ttacaaaaaa acaagttcgt ttaagttgca tgaatttact tcaaaaaaga attcatgaat
    11701 tgaacgaaat tgaacgctct gcccaagaat ctgccgcggg tgatactaaa agtagcatgg
    11761 gcgacaaaca tgaaacttca cgcgaactct tgcagcaaga acgtgaaatg gccggtcgaa
    11821 gaatggctga agcaattcga caaaaagaag aattggaacg catactccca gaacatgtat
    11881 tgaatcaagt tcaaacggga gctttagtcg aaacttcaat tggtactttc tttttcgcta
    11941 ctgcattagg ttttatcaca ctacacgaaa agaaaattgc tgttttatcc attgcttctc
    12001 ctattggaca agcgttaaaa gagaaacaac ttggtgatat cgtttctttt caaaacaaag
    12061 acattcgaat aataagcata gcataatgtt ctaggtttta ctcatatcta gagcatacct
    12121 cttgcatccc cggacttaag tccggggctt cagtccgggt aaaagttcta ttctttcaga
    12181 ttattttcaa cctctttgta aggtttgaat gttagattcg accaatacaa aaaaacacct
    12241 catgaaagca attttattta cgctcggttt ttccttattc gccaatttta tagtagctca
    12301 ggttcctaaa aagattattg ttgaacactt caccaatagt cgatgtagca tttgtgcaaa
    12361 tcgcaatcca ggcttttttg ataacctaag tcaacatccc gatgtgcttc acatttcgat
    12421 gtatccaagt tcgccctatc cgaattgcta tttcagtcaa caaaatgcta atgaaaatga
    12481 cggaagaacc aattactacc aagtgtatgg ctcaacaccg gtttttgtca ttcagggaga
    12541 ggtaattaat caatcttcca attattcttc tgctgcttta tttgaacctt atttaaatca
    12601 aacaagcccc atttcaatac aagtaaatca gatacaaagc agcgattcga ttatctcaac
    12661 catatcggtt aaaacagaag cagaaaattc gcttggagaa ctagttatct ttggagctat
    12721 tgctgaggat accgtatttt tctcctcacc aaatggagaa acagagcatt acaatgtgca
    12781 tcgtaaatcc ttgtatggtg attcgggtat tccgatctcg attccttcca caattggcga
    12841 ttcagttact ttacgaaaag ctatcgctaa aaattcaaac tggaattata ctcgcttaaa
    12901 atctgtaatc attattcaag aatcgtctac taaaacagta gttcaagctg ccgaatccga
    12961 cacaaacaat ggaattacaa cgggtcaaat taatttaact tgtaataaaa ttgacattta
    13021 tcccaatcca gttgagtctt tcctaactat taaaggatat gggacaaaca tgcagaatta
    13081 ctatttaatt aattcaacag gaataattca gaaacaggga ataatgaatc aagaaacaaa
    13141 acttgatttt accgaattac cacatgggat ttatttatta aaaattggaa ccgagatcca
    13201 taagatcatc cattaatttc cacgaaaccg aaatagtcca gggctttggt ttagattttc
    13261 aaacttgaat taattattcc tcccgcagcc tcggacttca gtccgagcag acttcccaaa
    13321 tgatttttca tactctcaat ttctcatcat actaaaatgt tgtgtannnn nnnnnnnnnn
    13381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13441 nnnnnnnnnn nnnnnnnnnn nnnnnncttc ccaaatgatt tttcatactc tcaatttctc
    13501 atcatactaa aatgttgtgt aagcgatagc agcgaaaagc ccgcaacgtg cgccattgcc
    13561 ttggcgaagg cgtagagtga ggacttgtag cgaatagcgc ggtttgaaaa cggagcttgc
    13621 ggagttttca aatacaccca aaaacaaaaa atccccgccg gaaaaccgac ggggatttaa
    13681 aaaaacctaa ttaccttatt ttaaaatttt actgtgcgaa ttacttcgtt tgagttagca
    13741 gagcgaacac tgattaagta tgttcctttt gctaaatctg aagttggaag catgtgacga
    13801 attgtgctgc tgctagccac ttcaacgttt tcgcgaatca ttacttgtcc aagtgtgttg
    13861 attattgaca cttcgaaagt ttcacctgtg ttgcttgtgc tcaagttaac acttaatgtt
    13921 tcgttgaatg ggttaggata cacactgatt tcggcaactt cgtttgcagt tgttgtattg
    13981 tcttgagatt gaacttgagc aatacgtgca gtagaagttg aagaacatcc tggttgagct
    14041 actacaattc catctacatc aaatccatcg gcagttccag ggaagtgtga gttactcatg
    14101 gctgatgcgt cagagatgcg aacatattgg aacatagttg tacctgaacc ttcgatgtcg
    14161 attgattgat ctaagcaata tgattcagaa agcatgtacc atgttgtgtt gtcaagcgat
    14221 acttcgatac gagcagtttc tggataatta tcgcatgatg ggttaccgta agatgtttcg
    14281 attaacatga agtcagcacc tggtttatta aacacaacat atccaagttt caagcttact
    14341 tttccgccaa atccaaggct tacaaaattt actacatcac tgtattgtgg agcacctact
    14401 gcattgtctt tgttgctacg tgctgctggt actgcaccac catttttcat tgtaccaggt
    14461 aagtaatcgt aagcttcaac tgcgaactca ttgcttactg tagaaagaac tggatttact
    14521 tcttcgccat tcaaacatac aataccatct aagtcatatc catcagctaa accaccgtaa
    14581 ggaaagtcaa tgcgagagat gtcgattagt tttacatatt gtgcccaagc taatccttgt
    14641 aagtcgaagt acgtgttctg acaaccttcg cctaagaaaa cccaatcaca attgttttga
    14701 gaagcaaatg cgcggatttt ttctggatag cgactgcagt tttgtgagtt gttaccgaaa
    14761 gtcgtttcta ctacataaat atcatctcca tcaccgttgt gaattggatt agcgaatttt
    14821 aatatgattt ctccaccaaa accaagtgct acaaagttat acgcagtaga tggtgttgct
    14881 acatcgctgt cttctggaga acctaaagca ttaatcaagt tctgacgagc tgcttcaatt
    14941 ggagttaaaa gatcgttagt tgttcctggg atgtaactca ttacttgaac tgcattacat
    15001 ccagctggtg aaggctcaac tacagttgta tcaccaccat cggttgtttc atcatcgtca
    15061 tcatcaccat tgtcgttgtt gtcatcacct tcatcgtcgt tatcatcatc accattgtcg
    15121 ttgttgtcat ctccttcatc atcgttatca tcatcgccat tatcgtcgtt gttttcgcct
    15181 tcatcgtcat tatcatcatc accattatcg tcgttgtctt caccttcatc atcattatca
    15241 tcatcatcat tacctgaagt gcaagttcca atgtgatcac cgtgatttaa gtgtgcttga
    15301 agtgcattgt aattaatcgt aatggtatga gaaccgccat ttccagtttg atggcaaatg
    15361 gttatcattt gattatttcc ctcattatta ttgtgcttaa gagtaccaag tggtgaatgt
    15421 gtatttactt ccgaattacg tgtttgcgtt tgaacctgag tagtatgatc tgtttgtgca
    15481 tttagtccac caattgtaat aattagaatg gccagaattc gagaaatacg tgtcatcata
    15541 atggtaagat taggttgttt gtttcccttt accgtaatcc gacagaaagt gtttcattct
    15601 tacataaaaa aaatacggct cttataagca aaaagcatat atctacctga ttgtgtgcga
    15661 ctttaatatt aatccatttt tcattctctt cacacaataa cccaatcctt acatatgtaa
    15721 tagttaaaat tcgtttgcca atacatatat gtgttttatt tgggcggtcg agcaggctat
    15781 ccgcttttag tcctctcgta cgccatagcc tctggcgaag gcgtacgatg cgggctaacc
    15841 gcttctatcc ttaccgctcg atactagtaa ctaaattccg ttctattctt aaatagctag
    15901 tcaattgatt catttcctta ttccaactcc tatcatcacc ccaaaattac catccaagga
    15961 gtattgcttc ttttccggac aatacatccg cgaaacaaat ccatctaaat acaaagcatt
    16021 attgcaacca gcttgtttaa agtattgtgc aaaatcgtat aaattaattt cctcctttga
    16081 aatagcaaac aagatatttc cattcggtaa tattcctaca ccatttcgaa tattcacatg
    16141 agatgaacct aatttaaact cggggtgaat tttcccatca atcaccaaca taggtcccga
    16201 ttgcgtagca taccgaatat tcggattgat tttaaattcc tcggttcgac aaactaccgc
    16261 ttttcgattt ttcaataaat aaaaaacacc attcggcttt aaataaaaat ttccattccc
    16321 tgatttagta ttcaatgaag taagtgtttt atactcctct atatacaatc ctaaaggtga
    16381 attatcttcg gtgtacatgc cgccattcat ggcaaattgc aatacttcac cacgactttc
    16441 cacaaaggtt tttaattgct gaatcgattt taatttattg cctgcagcat ctttccaata
    16501 aaactgtaca tcttgtttgg taggatttat ttcgagcgaa attattctag aatcagaatt
    16561 atctatcact attggtggag caccaaaact ccccaaaaca ctaactagta gtcctatcca
    16621 aacaagtaaa ataaaatacc tgaactgatt tattattccc agatgtttca ttattcatgt
    16681 cagctattta gtatttccaa ctctccaagg tggatttatt ctatttcccc cagccgtata
    16741 aaaaattaat tgatttccat ctaaatcttt taatcgtgct tctcgccaca accaaggttt
    16801 atcagttggc atttcatcca atttaatccc ttgagattga attttaccta cgtattcatc
    16861 caaattttcc aattcgaaat acacccaaat tccatctccc tgaggcaaag tatcaacccg
    16921 atgaaccgat aatgtagatc cgttatctgg acaaacaaac cgagcatatt cgggaatcga
    16981 ttgtacaatt aattcgagtc ccaacttggt ataaaattca attgcctgct tcacattcaa
    17041 aacaggaagt gttacctgat ttaaattcat cattatttat tcttaatgca ggatttatct
    17101 tccggattca aatacgtaca gaaatagcca cattcctgta ttacatcctt ataatattga
    17161 gtatccgaaa aacttgcttt catcaaatta aaataaatac ccgatttaaa atcgccttgc
    17221 tcttcttcac cttcggtatt cattaaatta tactcacctt ctaaccataa attatgctcg
    17281 cattttgcag ccatccatgc acatttagct ttaaattctt tattcgtact ccaagccata
    17341 gcttttaaat accaacgttg agcttcagca caatcataat aaggcgattt tttcctaggt
    17401 tcattatcgt atgaattcaa atgataggta tcgatataat cccaattcac aacagttgac
    17461 gacaccaaac gtgcattacc ataccaagtc atattataca aaccattggc atataaaaaa
    17521 gcattatttg ctttctcctg agcttctttc gaactcattg ttttattctt catctcaagc
    17581 atcttacttg caaatgttcg cttgttatat tttactttct gaaatttctg atgatcacaa
    17641 tcgtgacaat ctgtaattcc catgttaaat ggatttccta ataattctgt taatccacta
    17701 gttgaatcct tggcaaaact ctttatcgct aagtcgaaat tatatttata catctgccct
    17761 accgcctgaa cttcatataa ttcctgaatt tttaccggat aagatcctaa taaataattc
    17821 tgaattggac gcttatccgg gtgcttcata aaatcaa
//
LOCUS       DAOOQW010000031        17391 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_3986_length_17391_cov_5.749712, whole genome shotgun sequence.
ACCESSION   DAOOQW010000031 DAOOQW010000000
VERSION     DAOOQW010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 17391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17391
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3986_length_17391_cov_5.749712"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            127..1338
                     /locus_tag="PK281_03465"
     CDS             127..1338
                     /locus_tag="PK281_03465"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008634370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOY48431.1"
                     /translation="MIPFSPPHIDQEIIDEVTAALRSGWITTGPRTKQFEKDLSEYNG
                     NPNTLCLNSATAGLEIVLRWFGVGPGDEVIVPAYTYSATANVVMHTGARPVFCDVKAE
                     DFNIDPKKLESLITERTKVIMPVDFGGMPCDFDAINSFVKRADIQAQFKARGEVQEKL
                     GRILILSDAAHSIGARYQGKMTGSLTDVSVFSFHAVKNLTTAEGGAVALNLPEPFDNK
                     AIYDYLCIATLHGQNKDALAKMQKGNWKYDIIEPGYKCNMTDIMAAIGLVELRRYESK
                     TLPRRKQIFDRYKEVLSQYSWAELAPYETDEKISSYHLFPLRIRGIAEAKRDAIMQKI
                     FDKDVSVNVHFIPVPMMKFYKEMGYSIDQFPVTYDNFSREISLPVFIDLSDEQVEEVL
                     AAVIEAVETSI"
     gene            1340..1933
                     /locus_tag="PK281_03470"
     CDS             1340..1933
                     /locus_tag="PK281_03470"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007649295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOY48432.1"
                     /translation="MLKRLFDLFSSAIGILILSPILLSLWLIVVIDSKGGGFYRQVRV
                     GLNGRDFKLWKFRTMRIGADQKGLLTVGGRDPRVTRVGFYLRKYKLDELPQLFNVFVG
                     EMSLVGPRPEVRRYVDLYTQDQRQVLSIRPGITDYASIAYSNENDLLAASSDPEKTYI
                     EEIMPAKLALNKKYMDEKDVWKDLRILFITVGKVIRH"
     gene            2035..3495
                     /locus_tag="PK281_03475"
     CDS             2035..3495
                     /locus_tag="PK281_03475"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY48433.1"
                     /translation="MKLLNLKHPFSTLFFSALLLVSTSEQVLSQVYATSITVLSGGRD
                     GYTSCCGWSPLTDGLIGTNRNPSNALGAPQCSDAITPPANNNYYALGFGGSVQLTLGA
                     GFSNGPGNDLTICETTFGVTSACAGLYPDKVTVYASQDNCSWTNLGTYCDAGLSPIGI
                     DLGALTWAKYVRLTDASIRPPNTSANNTQDGFDLDGVIGINALTITPALPSIVSNASS
                     VESYTQGLRKNGTTVTAASSNVSKTLGAPQNTELATPVNYFTLGFGGQIVLKLSSTCY
                     RTNGTNEIVIYESSNSGDIIACEANPEKASVYGSLNGTNWVLLNSSICMNATLGIPAN
                     STLSIGGAYGIRYLKIIDVSNPAFFASPTADGFDLDGVSVRIGCPAVAFRLAEETSET
                     SSLDSETRSTELSIYPNPASSVIKTALTLPYNSNVSFLLYDYSGKLVKTQSQIVPAEE
                     EMQQTINIEDLPDGVYLLIAQTDQERIVKRFLKQAN"
     gene            complement(3572..4663)
                     /locus_tag="PK281_03480"
     CDS             complement(3572..4663)
                     /locus_tag="PK281_03480"
                     /inference="COORDINATES: protein motif:HMM:NF026121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4476 domain-containing protein"
                     /protein_id="HOY48434.1"
                     /translation="MKYSFLFMLLSCFALTASAQKSNLVFFAENGEKFTIVMNGLRYN
                     ETPQTNVRINDLMPAVYKVKAIFEDPTLGVVTKTIPLDPYLEYNYNIRPKKTTAVGKT
                     VKGMGNQVARDLNMKDSSDVKDSDSKENYVMRFVSQTNLAMPVQQYQPAPVQQQVVVA
                     PPPTYTPAPTGTVQQTTTTTVHSTGTPVQTSVVAPVGGVNMSVNDPDLGVNFNMSVGV
                     PVGGTVVTSGGTVQQTTTTTTYGTPVQQQVVQQQYVMPGYNGPIGCPWPMDQNAFGGA
                     VSTINGQSFESNKLQVAKQAFASNCMTSAQVGQIMQLFSFEDSRLDFAKFAYGHTYDL
                     GNYYQINSAFTFSSSVDELNDFIAAQPRR"
     gene            complement(4757..5794)
                     /locus_tag="PK281_03485"
     CDS             complement(4757..5794)
                     /locus_tag="PK281_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HOY48435.1"
                     /translation="MNIDLLKEICETPGAPGYEQRIRAVVLREVESMGDAVSVDNMGN
                     VTVFRKGRSDKKVMVGAHMDEIGFMVTHIDDKGFVRFTTLGGFDPKTLTAQRVIIHGS
                     EDIIGVMGSKPIHLMSPEERDKSVKISDFFIDTGLTADEVKAKVSIGNTITREREFIR
                     MGECFNCKSLDNRISVFILIETLRQLKNIPYDLYAVFTVQEEVGIRGAQTATLRIQPD
                     FGFGLDTTIAYDVPGSKEHEQVTKLGAGAGIKVMDASVICDPRMVSYMKDVASKNNIT
                     WQPEILTGGGTDTSGIQRMTAGGAIAGAVSIPTRHIHQVIEMVHQNDVRASIELLKCS
                     IEQLDQWNWSW"
     gene            complement(5817..7247)
                     /gene="pyk"
                     /locus_tag="PK281_03490"
     CDS             complement(5817..7247)
                     /gene="pyk"
                     /locus_tag="PK281_03490"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014937895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HOY48436.1"
                     /translation="MSKVQSKTKVIATLGPATSTKEVLEKLIIAGVDVCRINFSHGSY
                     EDHAKVLGYIREINEEHGFHTAILADLQGPKLRIGEVENNGVMLVAGHDLVITTKKAI
                     GTAERVYITYPQFPQDVQAGEIILIDDGKIVLEVKDTNREDEVVATVIHGGILSSKKG
                     VNLPNTKISLPCLTKKDIQDLNFALEHHVEWIGLSFVRRASDILELKGLIHKANKNAH
                     VVAKIEKPEAIDEIDEIIANTDAIMVARGDLGVEIPMENVPLIQKMLVKKCLEASKPI
                     IIATQMMESMITNYNATRAEVGDVANSVMDGADAVMLSGETSVGAHPVRVIETVQKIL
                     ARVEGFEGIYHKDHKQSSHKSRMITDAICYNAVTMAEQTGTSAIIAMTHSGYTAFRIS
                     SFRPKANIFIFTGNRALLNTLSLVWGVRGFFYDKMVSTDHTISDIKNILKKDGYINQD
                     ELIINVASIPMHEFGKSNMLKISFVE"
     gene            complement(7249..7671)
                     /locus_tag="PK281_03495"
     CDS             complement(7249..7671)
                     /locus_tag="PK281_03495"
                     /inference="COORDINATES: protein motif:HMM:NF033205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPExxxVDY family protein"
                     /protein_id="HOY48437.1"
                     /translation="MKKKFTLSNYVDYDFSLIGICSQEEDFKLCFFLNQALQLDFERI
                     RDIEILLPKQGNMVSFSCFEYTDLDSDLSWYLIANRHPASNLLPDQKQTDYLLRIVGE
                     QELIDTTQLVNQIKEIPCVLTAFVIPPQDVKNIENILL"
     gene            complement(7711..8433)
                     /gene="rnc"
                     /locus_tag="PK281_03500"
     CDS             complement(7711..8433)
                     /gene="rnc"
                     /locus_tag="PK281_03500"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HOY48438.1"
                     /translation="MSFLSAQFSKQRALYRSIRNLLGFWPGNIRLYEQAFRHRSVARE
                     IRAGVKDSNERLEFLGDAVLGAVIANYLFKKFPFKDEGFLTELRSRMVSRIYLNKLAL
                     KIGIDKLIQYDDSNRLYKSICGDTFEALIGAIYLDKGFEFTEKIILQRIIRFHVDMEE
                     LENQDLNFKSKLINWAQRQRKSIFFDAREETGADRQKLYYVTVLIDNEPKGEGVGYSK
                     KTAEQAASENACALMVNEIDLA"
     gene            complement(8437..9690)
                     /gene="fabF"
                     /locus_tag="PK281_03505"
     CDS             complement(8437..9690)
                     /gene="fabF"
                     /locus_tag="PK281_03505"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010136107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HOY48439.1"
                     /translation="MPLRRVVVTGLGAVTPLGNTVQDTWTALINGVSGAEMITHFDAS
                     KFKTRFACEVKNFDPSGVIDRKAVGRLDLFAQYALVAADQAYIDAGLNPDNFDPDRSG
                     VIWGSGIGGLETFFKEVYNFAKGDGTPRFNPFFIPKMIADIASGHISMRFGFRGPNFT
                     TVSACASSTNAIIDSMFYIRMGKADIMMTGGSEAAVNEPAMGGFNAMHALSERNDSFK
                     TASRPFDVSRDGFVMGEGGCALILEELEHALKRGATIYAEIVGGGASADAHHITAPHP
                     EGLGALNVMKAALEDAGMQPEDLDYINVHGTSTPLGDIAETKAILGVFGDHAYKMNIS
                     STKSMTGHLLGAAGALEAMASLLAVKHDIVPPTINQENLDPELDSKLNFTFNKAQKRT
                     VRAALSNTFGFGGHNASVIFRKYPA"
     gene            complement(9708..9944)
                     /locus_tag="PK281_03510"
     CDS             complement(9708..9944)
                     /locus_tag="PK281_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014166687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOY48440.1"
                     /translation="MSDISARVKAIIVDKLGVDESEVTPEASFTNDLGADSLDTVELI
                     MEFEKEFNIAIPDDQAENIGTVGDAITYIEKNSK"
     gene            10124..10687
                     /locus_tag="PK281_03515"
     CDS             10124..10687
                     /locus_tag="PK281_03515"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HOY48441.1"
                     /translation="MANVIIFGSGKGSNARRLINYFEGNPQVKISALVSDKPRRGFLD
                     ISYDHRINLEIIKGPELADAKWINHLKLMYRPDLIVLAGYMRLIPAEFIQAFPNKIIN
                     LHPALLPEFGGKGMYGHHVHEAVIAAGRTETGISIHRVNERYDEGEILFQAQCPVFPD
                     DTVDTLSERIHQLEHEYLPQLVEKILS"
     gene            10712..11173
                     /gene="rnhA"
                     /locus_tag="PK281_03520"
     CDS             10712..11173
                     /gene="rnhA"
                     /locus_tag="PK281_03520"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007095948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HOY48442.1"
                     /translation="MHIELYTDGSSRGNPGPGGYGLILRAGPHYKEMSQGFRLTTNNR
                     MELMSVIVGLESLKNPGSQVIVFSDSKYVIDSITKGWVFGWVKKGFAGKKNKDLWMRF
                     LKIYPKHQVKFQWVKGHNDHPQNERCDALAVAAATGGNLQVDLGYENGEAG"
     gene            complement(11170..13896)
                     /locus_tag="PK281_03525"
     CDS             complement(11170..13896)
                     /locus_tag="PK281_03525"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HOY48443.1"
                     /translation="MTPQLRLSFATAFLSALILVPSCKNHRDKSGKDSQSFLDSAVYA
                     NVDSIVNLPEIDFSDMQFSDESKRYNPSAKRVNDILHTRLEVSFDLARQQLYGKARID
                     IKPYYYPTDSLILDAKAFDVQRVALVGKGDVMTDLAYKNDDVQLHIYLGKSFTRSEPY
                     TIFIQYTANPQRVKTPGSSAISDAKGLYFIDPKDEDPSKPTQIWTQGETESSSCWFPT
                     IDKPNEKMTHEILITVPEKYSTLSNGLKTLSKKNSDGTRTDTWKMDQPNAPYLVMMAI
                     GEFAIVKDTWRKIAVDYYVEKEFEPYARKIFGNTPEMLEFFSTRMGVDYPWPKYSQVV
                     VRDYVSGAMENTSATLFGEFVQQTPAEMIDNDFEDVIAHELFHHWFGDLVTCESWSNL
                     PLNESFATYGEYLWNEYKYGRDEADHHLQGDLNTYIEESFQKKVDLIRFDYDFREDMF
                     DRHSYQKGGCVLHMLRKYLGDEAFFEGLKTYLTDNRFQPAEIHHLRLAFEKVTGEDLN
                     WFFNQWFLDKGHPILNAKISWDEATSESVIEVEQTQNRESSPMYKLPLDVDIYEKGNI
                     RRTRIVVDSMKTTIRLKAAVRPDLVLLDAEHQLLGKLDQHLTLAEAQTLFLQGKRYRD
                     RFQAIDLLLDNAAEPGVTELLKMALRDPFWNIRLYSLNRMDEVIAQNPEDFKSILMQL
                     AAKDEESSVRAAALEKVAGLSSGDATVFPVFEKALSDSSNLVQGAALGAMYAADPERT
                     KDALGKLESTAKGDLLTSIAAIYATSGTPGKFEFLKSAYEKVNNPNDKYVFIQVMGKY
                     ALSQDEATMDGSVPTFENIARKANAWWLRLSGIQVLAEFQMRYDAKVDALSSEIQTMI
                     EKGTAVSIVQQKELERTAYKKKVTEVDIILDTIRSEEKDPNLTRILMMMGGH"
     gene            14158..>17391
                     /locus_tag="PK281_03530"
     CDS             14158..>17391
                     /locus_tag="PK281_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48444.1"
                     /translation="MKYFYIVFLGFLGSQLSAQLFVPVTGVNNYTVCSGTLYDSGGQF
                     GNYSDNSNGYTVLTPSTAGNMVQIGGTLIGENCCDFMQIFNGSGLGGNLLWSGNTSVG
                     AVPTITSTTGPLTVQFITNGNVVGAGFALDIICVPPATLTVPETGVNSYTVCSGTLYD
                     SGGPSGSYANNSNGYTILNPSNPGYMIRVNGSSIGENVFDVIQIYNGAGLTGNLLWEG
                     SLGFGSIPTITSTAGPLTVKFTSNASTTGNGFALDINCDPPATFTVPVSGVNNYTVCS
                     GTLYDSGGPFGNYSDNSNGYTVLNPSNPGNMVQIGGSFNGQNCCDFLQIFNGVGLTGN
                     LLWSGIASIDGVPTITSTAGPLTVQFISNGNTVGAGFALDINCVPPATLTVPVTGVNT
                     YTVCSGTLYDSGGPSGSYANNSDGYTILNPSNPGYMMSVSGSSIGENVFDVIQIYNGA
                     GLTGNLLWEGSPGLGSIPTITSTTGPLTVKFTSNASTTGNGFALNLICTPPTTFTVPT
                     SGVNNYTVCSGVLYDSGGPLGNYSDDSNGYTTLYPSNPGNMVQIGGTFTGQNCCDFMQ
                     IFNGVGLGGNLLWSGNPSVGVVPTITSTSGPLTVQFISNGSVVGAGFALDIICLPPII
                     FTVPSSGNNEYTVCSGILYDSGGPTGSYVNNSNGYTILNPSNPGYMMTVSGGSIGENV
                     FDVIQIYNGAGLTGNLLWEGSPGLGSIPTITSTAGPLTVKFTSNASTTGNGFALDLIC
                     TPPTTFTVPTSGVNNYTVCSGVLYDSGGPLGNYSDDSNGYTTLYPSNPGNMVQIGGTF
                     TGQNCCDFMQIFNGVGLGGNLLWSGNPSVGVVPTITSTSGPLTVQFISNGSVVGAGFA
                     LDIICLPPIIFTVPSSGNNEYTVCSGILYDSGGPTGSYVNNSNGYTILNPSNPGYMMT
                     VSGGSIGENVFDVIQIYNGAGLTGNLLWEGSPGLGSIPTITSTAGPLTVKFTSNASTT
                     GNGFALDLICTPPTTFTVPTSGVNNYTVCSGTLYDSGGPFGNYSDDSNGYTVLNPSNP
                     GSLMQIGGSFNGQNCCDFLNIFNGVGLGGNLLWSGNASAGVVPTITST"
BASE COUNT         5081 a         3566 c         3546 g         5198 t
ORIGIN      
        1 attttagcta cggaataggc tatttacaag gaataattga tttcttcatt ttaaaaaaat
       61 cacccggaaa acgacataca tcattgtctc gataaatcaa ttttatccgg aaaaaaaatc
      121 tgaaccatga tcccattttc acctccccat atcgaccaag aaattataga tgaagtaaca
      181 gcagcgcttc gttccggctg gattacaaca ggtccacgca ctaaacaatt cgaaaaagac
      241 ctcagcgaat acaacggtaa tccaaacacc ctttgcttaa attcagccac tgccggactc
      301 gaaatcgttc tgcgttggtt cggtgttggt ccgggtgatg aagtaattgt ccctgcctac
      361 acctattctg ctaccgctaa tgttgtgatg cataccggtg ctcgtccggt tttttgcgat
      421 gtaaaagcag aagatttcaa tattgatccg aagaagttag aatcgctcat aactgaacga
      481 actaaagtca ttatgcccgt tgactttggt ggtatgcctt gtgattttga tgcaattaat
      541 tcgtttgtta agagagctga catacaggct caatttaaag cacggggaga agttcaagag
      601 aagttaggta ggatattaat cctatcggat gcagctcatt caattggagc gcgctatcaa
      661 ggtaaaatga ctggctcttt aactgatgta tctgtctttt catttcatgc tgtaaaaaat
      721 ctaactacag ccgaaggtgg tgccgttgca ttaaatctac cagagccttt cgataacaag
      781 gctatctacg attatctatg tattgccacc ttgcatggac aaaacaagga tgcccttgct
      841 aaaatgcaaa agggtaactg gaaatacgat atcatcgaac ccggttataa atgcaacatg
      901 actgatatca tggctgcaat tggcttggtt gaacttcgtc gatatgaatc gaaaacgcta
      961 ccccgcagaa agcaaatttt tgatcgatat aaggaagttt tgagtcagta ttcttgggca
     1021 gagttagcac cttacgaaac ggatgaaaaa atatcttcct atcacttatt tccactgaga
     1081 attcgaggta ttgcagaagc taaacgagat gcaatcatgc aaaagatatt tgataaggat
     1141 gtttcggtaa atgttcattt cattcctgtt ccaatgatga aattttacaa ggaaatgggt
     1201 tattcgattg atcaatttcc agttacgtat gataatttta gtcgtgaaat ttcattaccc
     1261 gttttcatcg atttaagtga tgaacaagta gaagaagttc ttgctgctgt tattgaagct
     1321 gtagaaacat cgatataaca tgcttaaacg cctgtttgat ttattttcct ctgccattgg
     1381 aatattgatc ttatctccca tattacttag tttatggttg atagttgtga tagattcaaa
     1441 aggaggggga ttttatcggc aggttcgagt aggacttaat ggaagagatt tcaaactgtg
     1501 gaaatttcga accatgcgca ttggtgcaga tcaaaaaggg cttttaacag ttggcggtag
     1561 agatcctcgt gtaacgcgtg taggcttcta tttacgcaaa tacaagctcg atgaattacc
     1621 acaacttttc aatgtttttg ttggtgaaat gagcttagtt ggaccgcggc ctgaagttcg
     1681 acgttatgta gatttatata ctcaagacca acgtcaagtt ttatctattc gtcccggaat
     1741 taccgattat gccagcatag cttattcgaa tgaaaacgat ttattagcag cttcctctga
     1801 tccggagaaa acgtatatcg aggaaattat gcctgcaaaa ctagccttga ataaaaaata
     1861 catggatgaa aaagatgtgt ggaaagattt acgcatcctt tttatcactg ttggtaaagt
     1921 gattcgtcat taacgagtca taaagattta ttatctataa ttttttataa tttggtaatc
     1981 aactatttcg atagtttatt atcgcattaa tacagcaaac taacccattc atttatgaaa
     2041 ctactaaacc taaagcatcc attcagtaca cttttctttt ctgctttatt acttgttagc
     2101 actagcgaac aggtactatc tcaagtttat gcaacttcaa ttacggtttt atcaggagga
     2161 agagatggct atacatcatg ttgcggttgg tcgccattaa cagatggctt aattggtacg
     2221 aatcgaaatc ctagcaatgc acttggagca cctcaatgca gtgatgctat cacacctcca
     2281 gcaaataaca attactatgc acttggattt ggcggatctg tccaactaac attaggagca
     2341 gggttttcaa atggtccagg aaatgacctg actatttgcg aaacaacatt tggagttact
     2401 tctgcttgtg caggtttgta tccagataaa gtaactgtat atgcttctca agacaattgc
     2461 tcatggacta acttaggtac atattgcgat gctggattaa gtccaatagg aattgattta
     2521 ggtgccttaa catgggctaa atatgttcgt ttaacagatg catcaattag gccaccgaat
     2581 acatcggcaa acaatacaca ggatggattt gatcttgatg gagtaattgg aataaatgca
     2641 ctaacgatta cacctgcact tccatcgatc gtatcgaacg ctagttctgt agagagttat
     2701 acgcaaggat taaggaaaaa tggcacaaca gtgacggcag caagctcaaa tgttagtaaa
     2761 acactaggag ctcctcaaaa caccgaattg gcaactcctg ttaattattt cacactcgga
     2821 tttggtggtc agatagtttt gaaattatca tctacttgct atcgaaccaa tggcacgaat
     2881 gaaattgtca tttacgaaag ttctaactcg ggtgatataa ttgcttgtga agctaatccc
     2941 gaaaaagcaa gcgtttatgg atcattaaat ggaacaaatt gggtactgtt gaattcatcc
     3001 atatgcatga atgctacttt aggaattcct gcaaattcaa cactttcaat tggtggcgct
     3061 tatggtattc gttatctaaa aatcatagat gtttctaatc cggctttttt tgcatctcca
     3121 actgccgatg gttttgattt agatggagtt tcggtgagaa ttggttgccc agctgttgcc
     3181 ttccgattag cagaagagac ttccgaaact tcaagtctag attcagaaac acgatcaacc
     3241 gaacttagta tttatcctaa tcctgctagt tcggttatta aaactgctct gactttacct
     3301 tataattcaa atgtatcctt cttactttat gattattcgg gtaagctggt aaaaacccaa
     3361 tctcaaattg ttcctgctga agaagaaatg caacaaacaa ttaatataga agacttacct
     3421 gatggagttt atcttctaat cgctcaaact gaccaagaaa gaattgtaaa acgattttta
     3481 aaacaagcaa attaaattct ccagtggaat aaaatcaaaa gccccgaaaa tcctagaatt
     3541 tccggggctt tttaattatg tgatttgaat cttaccggcg aggttgagct gcgataaaat
     3601 cattcagttc atctacacta gaagaaaaag taaatgcgct attgatttgg tagtagttac
     3661 ctaaatcgta agtatgacca tatgcgaatt tagcgaagtc taaacgagaa tcttcgaaag
     3721 agaacaactg catgatttga ccaacttgag ctgaagtcat acagttacta gcgaaagctt
     3781 gtttagctac ttgaagtttg ttgctttcga aactttgtcc gttgatagtt gaaacagcac
     3841 caccgaaagc attttgatcc attggccaag gacaaccgat tggaccattg taacctggca
     3901 ttacatattg ttgttgaact acttgttgtt gaacaggagt tccataagta gtagttgttg
     3961 tagtttgttg aacagtacca ccagaagtaa caacagtacc accaactgga acaccaacac
     4021 tcatgttaaa attaacaccc aaatctggat catttacact catattaact cctcctactg
     4081 gagcaacaac tgaagtttga acaggtgttc cagttgaatg aacggttgtt gtagttgttt
     4141 gttgaactgt tcctgttgga gctggggtat atgttggagg aggtgctaca actacttgct
     4201 gttgaactgg agcaggttga tattgttgaa ccggcatagc aagatttgtt tgtgatacga
     4261 atcgcattac ataattttct tttgaatcag aatctttcac atcagatgaa tctttcatgt
     4321 tcaaatcacg agcaacttga tttcccatac ccttaactgt tttaccaaca gcagtagttt
     4381 tcttagggcg aatgttatag ttatattcta aataaggatc taaaggaatt gttttagtta
     4441 ctacgccaag tgtaggatct tcgaaaatcg ctttcacttt ataaacggct ggcatgaggt
     4501 cattaatacg aacattggtt tgaggagtct cgttataacg aagaccattc atcacaattg
     4561 taaatttttc accattctct gcaaagaata caagattgga tttttgagcg gatgcagtta
     4621 aagcgaaaca gcttaacagc ataaagagga aagagtattt cataggattt ttgttttgga
     4681 cgcggcggct tttcaaagcc tgtgccaaag ataaatcaat agtttcaaaa aattaatgtt
     4741 gtattaaata gttgaattac caagaccaat tccactgatc taattgttcg atgctacatt
     4801 tcaataattc aatagaagct cttacatcgt tctgatgaac catttcaatt acttgatgaa
     4861 tatgtcgcgt tggaatacta acagctccag caatagctcc acctgcagtc attcgttgaa
     4921 tgccacttgt gtcggtaccg ccaccggtga gtatttcagg ttgccatgtg atattatttt
     4981 tagacgctac atctttcatg taactcacca tacgtggatc acaaataaca gaagcatcca
     5041 ttactttaat gcctgcaccc gcacctaact tagttacttg ctcatgctct tttgaacccg
     5101 gaacatcgta ggcaatagtt gtatctaacc caaatccgaa atcaggttga atgcgcaacg
     5161 ttgcagtttg tgctcctcga attcctactt cctcctgaac agtaaacacc gcgtacaaat
     5221 cgtaagggat atttttaagc tggcgaagtg tttctatgag aatgaaaaca gaaatccggt
     5281 tatccaaact tttacaattg aaacattcac ccatgcgaat gaattcgcgt tcacgcgtga
     5341 ttgtattacc aatgctgact ttggctttca cttcatcagc cgttaaacct gtatcgataa
     5401 agaaatcgga gattttaaca gatttatcgc gctcttcggg tgacattaaa tgtattggtt
     5461 tagaacccat caccccaata atatcttccg atccatggat gataacacgt tgagcagtta
     5521 atgttttagg atcaaatcct cccaatgtgg taaagcggac aaatccttta tcatcaatgt
     5581 gcgtcaccat gaatccaatt tcatccatgt gtgctccgac catcactttt ttatcggaac
     5641 ggcctttccg gaatacggtt acattgccca tgttgtcgac gctaactgca tcgcccatag
     5701 actctacttc gcgcaatacc acggcacgaa tgcgttgctc ataaccagga gcacccggtg
     5761 tttcacaaat ctcttttagc aggtcgatat tcatgctaca aaagaaagta aaatcctcat
     5821 tcaacaaaac tgattttcag catattcgat ttgccaaatt cgtgcatggg aatactggcc
     5881 acattgatga tcaattcatc ttgattgata tacccatctt ttttaagaat attcttaatg
     5941 tcggatatgg tatgatcggt acttaccatt ttatcgtaga agaatcctcg aacaccccaa
     6001 actaagctta gcgtattgag taaagctcgg tttccagtaa aaatgaaaat gtttgcttta
     6061 ggtcggaaac ttgaaatacg gaatgcggta taaccagagt gagtcatagc aatgattgct
     6121 gatgtacctg tttgctcagc catagtaacg gcattgtagc agatagcatc agttatcata
     6181 cgcgatttat gagaactttg cttgtgatct ttgtgataaa tcccttcgaa tccttctacc
     6241 cgagcgagaa ttttctgaac ggtttcaata acccgaactg gatgcgctcc tacagaagtt
     6301 tctccactta gcataactgc atcggcacca tccatgaccg aattagctac atctcccact
     6361 tctgctcgtg ttgcattgta gttggtaatc atcgattcca tcatttgtgt ggcgataatg
     6421 attggtttag atgcttcgag acatttcttc accaacattt tctgtatgag tggaacgttt
     6481 tccattggaa tttctacacc caaatcaccc cgtgcaacca taattgcatc cgtatttgcg
     6541 ataatctcat ctatctcatc aatcgcttca ggcttctcaa ttttggccac cacatgtgca
     6601 tttttattcg ctttgtggat gagtcctttt aattctaaga tatcggaagc acgacgcacg
     6661 aatgaaagtc caatccattc cacatgatgc tccaaggcaa aattcaaatc ttggatatct
     6721 tttttagtca agcacggaag tgaaatttta gtattcggta aattcactcc ttttttagac
     6781 gataaaattc caccgtgaat tacagtagca actacttcgt cttctcggtt tgtgtcctta
     6841 acttctaaaa cgattttacc atcatcaatc aaaataatct ctcccgcttg aacatcttgc
     6901 ggaaattgag gataagtgat ataaacacgc tcagctgttc caatcgcttt ctttgtggtg
     6961 ataactaaat cgtgtcctgc aactagcatt acaccattat tctccacttc tccaattcgt
     7021 aatttgggac cttgtaaatc ggctagaata gctgtgtgga aaccatgttc ttcgttgatt
     7081 tctcgaatgt aaccaagcac tttagcatga tcctcatatg aaccatgact aaaattaatt
     7141 cgacatacgt caacacccgc aatgattaat ttttcaagaa cttctttggt tgatgttgca
     7201 ggaccaagtg ttgcgataac cttggttttg ctttgcactt tgctcatttt agagtaatat
     7261 attttcgatg tttttaacgt cttgaggagg aatcacaaaa gctgtgagga cacaggggat
     7321 ttccttaatt tgatttacca attgagttgt atcgatgagc tcttgctcac caacaattcg
     7381 gagtaaataa tcggtttgtt tttgatcagg aagtagattg ctagctggat gacggttggc
     7441 aatcaaatac caacttaaat cagaatctaa atcggtgtat tcgaaacacg aaaaactaac
     7501 catgtttccc tgtttgggta gcagtatttc tatgtcgcga atgcgttcaa aatcgagttg
     7561 taatgcttga ttgagaaaga aacagagttt aaaatcttcc tcctgactac atatcccgat
     7621 aagcgagaaa tcgtaatcca cataattgct gagggtgaac ttctttttca tgatccgcat
     7681 gcgaagatac gtcacattgt tgaaaaaaca ctaagctaaa tcaatctcat tgaccatcag
     7741 tgcacaagca ttttctgaag ctgcttgttc ggctgttttc ttcgaataac caacgccttc
     7801 acctttgggt tcattatcga ttaacacggt aacataatag agtttttgtc gatcggcacc
     7861 tgtttcttcg cgtgcatcga agaagatgct ttttcgttga cgttgagccc agttgatgag
     7921 cttcgatttg aaattcagat cctgattctc gagttcttcc atatccacat gaaaacggat
     7981 aatgcgttga agaataattt tctcggtaaa ctcaaagcct ttgtcgaggt atatcgcacc
     8041 gattagcgct tcgaacgtat cgccacaaat cgatttatag agtcgattgg aatcatcgta
     8101 ttggatgagt ttatcgattc ctatttttag tgcaagttta tttaggtaga tacgactcac
     8161 catacgggag cgcaattcgg tgagaaaacc ttcgtcttta aaaggaaatt tcttaaacaa
     8221 gtaattagcg ataacagctc caagaacggc atcacctaaa aactcaagac gttcgttaga
     8281 atctttaaca ccagcacgga tctcacgcgc tacagagcgg tgacgaaagg cttgttcgta
     8341 aagacgaatg tttcctggcc aaaatcctaa tagatttcgg atggaacgat aaagcgcacg
     8401 ttgcttagaa aattgtgcgg aaagaaaact cacacgttaa gctggatatt tccggaagat
     8461 aacggaagca ttatgcccac cgaaaccaaa agtattgctc aaagcagccc ggacagtccg
     8521 tttttgtgct ttgttgaacg taaagttcag cttagaatcc aattccggat ctaagttttc
     8581 ctgattgatg gttggtggaa cgatatcgtg tttcaccgcc agtaaggatg ccatagcttc
     8641 caaagctcca gctgcaccta aaaggtgacc tgtcatggat ttggtggaac tgatgttcat
     8701 tttataagca tgatcaccaa aaacacctag gattgctttt gtttcggcaa tgtcgcccaa
     8761 tggagtagaa gtcccatgga cattgatata atctaaatct tcgggttgca tgccagcatc
     8821 ttccaaagca gctttcatca cattgagagc accaaggcct tcaggatgag gagctgtgat
     8881 atgatgtgca tctgcagaag caccaccacc cacaatttca gcataaatgg tagcgccgcg
     8941 ttttaaggca tgctctaatt cttctaaaat caatgcgcaa ccaccttctc ccattacgaa
     9001 tccatcacgg gaaacgtcga aaggacgaga agccgtttta aacgaatcgt tacgttcaga
     9061 aagagcgtgc atggcattaa aaccacccat tgccggttca ttcacagctg cttcagaacc
     9121 acctgtcatc ataatatctg ccttacccat tcgaatgtag aacatcgagt cgatgatcgc
     9181 attggttgaa gaggcgcaag cagatacagt tgtgaagttt ggtccacgga aaccgaaacg
     9241 catcgaaatg tgtccggaag caatgtcagc aatcatttta ggaatgaaga aaggattgaa
     9301 acgaggtgtt ccatctcctt ttgcgaaatt gtaaacttct ttgaagaaag tttccaatcc
     9361 accaattcca gaaccccaaa taacgccaga acgatctgga tcgaaattat cgggattcaa
     9421 cccagcatca atgtatgctt gatcggcagc gaccaatgca tattgagcaa agaggtcaag
     9481 tcgacccacg gcttttcgat cgattactcc tgatggatcg aagttcttta cctcgcaagc
     9541 aaagcgggtt ttgaacttcg atgcatcaaa atgggtaatc atttctgcac cactgacacc
     9601 attgatcaat gccgtccagg tatcctgaac agtattgcca agcggtgtaa cagcaccaag
     9661 accagtaacg acgactcttc tcaaaggcaa aaaatgaaag gggtagctta cttcgagttc
     9721 ttctcgatgt aagtaattgc atcacctaca gtaccgatat tctcagcctg atcatctggg
     9781 atagcaatat tgaattcttt ttcaaattcc atgatcaatt caacggtatc gagtgaatcg
     9841 gcacctagat cattggtgaa ggaagcctct ggggtaactt cactttcgtc tactccaagc
     9901 ttatctacga taatagcttt tacgcgggca gagatgtctg acattttggt tgtgttatgt
     9961 aaaattcgct gcaaagaaag agtttactca aatactatca aaatcttttt ttcaacgcat
    10021 cgtaagaggc cgactcctat tgagaactag aagataaaac tattccattt gatgcattat
    10081 aatggtaaga atgggctatt ttcgcccctc aaaacggaag taaatggcga atgtgatcat
    10141 cttcggctcg ggtaaaggaa gcaatgctcg gcgattgata aactactttg aagggaatcc
    10201 tcaggtgaag attagcgcat tagtgagtga taaaccccga cgtggttttc tggatatttc
    10261 atacgaccat cgcatcaacc tagaaataat caaaggtcca gagcttgccg atgctaaatg
    10321 gatcaaccat ctaaaactca tgtatcgccc cgatttaatc gtattagccg gttacatgcg
    10381 tttgattcct gccgaattca ttcaagcatt tccgaataaa atcatcaacc tccaccctgc
    10441 cctattgccc gaatttggtg gaaaaggaat gtatggtcac catgttcatg aagcagtgat
    10501 tgcggcagga agaaccgaaa cgggtataag tattcatcga gtaaatgaac gttacgatga
    10561 aggagagatt ctttttcaag cccaatgtcc tgtttttcca gatgatacgg ttgacacact
    10621 ctcagaacgc attcatcaac tcgaacatga gtacttacct caacttgttg aaaagatatt
    10681 aagctaatcg ccctatcatc atccaaccac catgcatatt gaactttaca ccgatggttc
    10741 ttctcgcgga aatccggggc caggtggtta tggattaatc cttcgagctg gaccacatta
    10801 taaggaaatg agtcagggtt ttaggctaac cactaataac cggatggaat taatgagtgt
    10861 gatcgttggc ttggaatcgt tgaaaaatcc gggttctcag gtgattgtgt tttcggattc
    10921 gaagtatgtg attgattcga ttaccaaagg ttgggtgttt ggctgggtga agaaaggttt
    10981 tgcagggaag aagaataaag acttgtggat gcgattttta aaaatttacc ccaagcacca
    11041 ggtgaaattc cagtgggtaa aaggccataa cgaccaccca cagaacgaac gctgtgatgc
    11101 attagctgta gcagctgcaa ctggcggaaa tctgcaggtg gaccttggat atgaaaatgg
    11161 agaagcaggt taatgacctc ccatcatcat caaaatacgt gttaggttag gatctttttc
    11221 ctcagaacga attgtatcga ggatgatgtc cacttcagtg acttttttct tgtaagcagt
    11281 acgctcaagt tccttttgtt gaactataga aacagcagtg cctttttcaa tcattgtttg
    11341 aatctctgaa gaaagtgcat ccactttagc atcgtaacgc atttggaact cagctaaaac
    11401 ttggattccc gataaacgta accaccaagc atttgctttg cgagctatat tttcgaaagt
    11461 tggaacagaa ccatccatag tagcttcatc ctgagaaagg gcgtatttac ccataacttg
    11521 aataaacacg tatttatcgt ttggattatt aaccttctcg taagccgatt tcaagaattc
    11581 aaatttacct ggtgttccag aagtagcgta aatagcggca atggatgtga gtaaatctcc
    11641 tttagccgtt gattccagtt ttcctaaggc atcttttgta cgctccggat cggcagcata
    11701 catggcacct aaagcagcac cttgaactag attagaacta tcgctcaaag ctttctcaaa
    11761 tacaggaaaa acagttgcat caccagaact caaaccagct actttttcta aagcagcagc
    11821 acgaacagat gattcttcat ctttagcagc taattgcatc aaaatggatt tgaaatcttc
    11881 tggattctgt gcaatcacct catccattcg attgagcgag tacaagcgaa tgttccagaa
    11941 tggatctctc aatgccattt tcaacaattc ggtaactcct ggctcggcgg cattatctaa
    12001 caataaatca attgcttgaa agcgatctcg gtagcgtttt ccttgaagaa ataaggtttg
    12061 tgcttctgct agtgtaaggt gctgatcgag tttacctaat aattgatgtt cagcatctag
    12121 caatactaaa tcgggacgaa ctgcggcttt caggcgaata gtagttttca tactatccac
    12181 cacaatgcgt gtacgacgaa tatttccttt ctcgtaaata tctacatcca aaggcaattt
    12241 atacatcgga cttgattccc gattctgtgt ttgctctact tcaataaccg attcagaagt
    12301 tgcttcatcc caagaaattt tagcatttag gataggatgt cctttatcga ggaaccattg
    12361 attaaagaac caattcagat cttcacccgt tactttttcg aaagccaatc gcagatgatg
    12421 aatttcggca ggttgaaagc gattatcggt tagataggtt ttcaatcctt cgaaaaatgc
    12481 ttcatcacct aggtatttgc gcaacatgtg aagaacacat cctccctttt ggtaagaatg
    12541 gcggtcaaac atatcctccc ggaaatcata atcgaagcga attaaatcaa ctttcttttg
    12601 aaatgattct tcgatgtagg tattgaggtc gccctgaagg tgatgatctg cctcgtcgcg
    12661 cccgtattta tactcattcc agagatattc accgtacgta gcaaacgatt cgttgagtgg
    12721 tagattagac caagattcgc aggtaactaa atcgccaaac caatgatgaa aaagttcgtg
    12781 agcaatcaca tcttcaaagt cgttatcgat catttcggct ggtgtttgct gaacaaattc
    12841 accgaaaagc gtggcggatg tattttccat cgcacccgaa acataatcac gcactacaac
    12901 ctgagagtat ttaggccaag gataatctac tcccatgcgg gttgaaaaga attctagcat
    12961 ttcgggtgta ttaccgaaaa ttttacgcgc atatggttcg aattcctttt ctacgtaata
    13021 atcaacagca atttttcgcc aagtatcttt cacaatcgcg aattccccaa tggccatcat
    13081 caccaaataa ggagcattgg gttgatccat tttccaagta tcggtgcgag taccgtcact
    13141 gtttttcttg cttaaggttt tgagtccgtt gctgagtgtg ctatattttt cgggaaccgt
    13201 gatgaggatt tcatgcgtca ttttctcatt cggtttatcg atagttggaa accagcaaga
    13261 agacgattcg gtctcacctt gagtccatat ttgagtaggc tttgatggat cttcatcttt
    13321 cggatcgatg aaatacaagc cctttgcatc ggaaattgca gaactgcccg gtgttttaac
    13381 acgttgcgga ttagctgtgt attgaataaa aatcgtgtac ggttcgctac gcgtaaagga
    13441 tttacctaaa taaatatgca actgaacatc gtcgtttttg taagctaagt cggtcattac
    13501 atcgcccttt ccaaccagtg cgacacgctg aacatcaaac gctttcgcat ccaaaatcaa
    13561 ggaatcggtt ggataataat agggtttgat atcgatacga gctttcccat aaagctgctg
    13621 acgtgcaagg tcgaaactca cttccaagcg ggtatgtaaa atgtcattta cacgtttggc
    13681 agatggatta tagcgttttg attcatccga aaattgcatg tcgctaaagt cgatttcggg
    13741 caggttgaca attgaatcga cattcgcata aacagccgaa tcgaggaaag attgagaatc
    13801 ttttccggat ttatcgcggt ggtttttaca agaaggaacg agaattagcg cgctaagaaa
    13861 agcagttgca aaggaaagac ggagttgggg cgtcattgag ttgattaaaa atggagggta
    13921 aatataacgg gattcgtttg attctgttgt tagcacggct acgagctaat ttactatctg
    13981 aagtttacac tttatgcttc aaactcacga aatgaatggt tttcggaatt catttgaaaa
    14041 ttttaaccct ctcatgatta tcatagaaca agcttaaacg ctcttttaac cctttaggaa
    14101 atccacttta catcttctat aaatacccta tttttgtttg attttgcgac acaatacatg
    14161 aaatatttct atatagtttt cctaggattt cttggctctc agctatcagc tcaactattt
    14221 gttcctgtaa caggagtcaa taactacaca gtttgctcgg gtactttata cgattcgggc
    14281 ggacaatttg gaaattacag cgacaattca aatggatata cggtgcttac tcccagcaca
    14341 gcaggaaaca tggtgcaaat cggtggtacg ctcattgggg agaactgctg cgatttcatg
    14401 caaatattca atggttcagg attgggtgga aatttacttt ggtctggtaa tacttccgtt
    14461 ggggcagtac caaccattac atccacgaca gggccgctaa cggtacagtt tattaccaat
    14521 ggaaatgttg ttggagcagg cttcgccttg gatattatct gtgttccacc ggcaacactc
    14581 acggtacctg aaacaggagt gaattcttac accgtttgtt cagggacttt atacgattct
    14641 ggggggcctt cgggctctta cgccaacaat tctaatggat atacgattct caatccaagc
    14701 aacccaggct atatgattag agtaaacggt agttccattg gggaaaatgt attcgacgtt
    14761 atccaaatat acaatggagc tggacttaca ggcaacttgc tttgggaagg tagtctagga
    14821 tttggctcta ttccaaccat tacatccacc gctggtcctt taacggtgaa atttacctcg
    14881 aatgcaagca ctacaggaaa tggctttgcc ttggatatca attgtgatcc tccggcaact
    14941 ttcactgtac cagtatcagg agtcaataac tacacagttt gctctggtac tttatacgat
    15001 tcgggaggac cttttggaaa ttacagtgac aattcaaatg gatataccgt gctcaatccc
    15061 agtaatcctg gaaacatggt gcagataggt ggctctttca acggacagaa ctgctgcgac
    15121 tttttacaga tattcaatgg tgtaggacta actggaaatt tactttggtc aggaattgct
    15181 tctatagatg gagtgccaac cattacctct actgctgggc cattaaccgt gcagtttatc
    15241 tccaatggaa ataccgttgg agcgggtttc gctttagata ttaactgtgt acctccggca
    15301 acattaacag tacctgtaac aggagtaaat acctatactg tgtgttcagg caccttatac
    15361 gattcgggtg gaccatctgg ctcttacgct aacaattctg atggatatac gattcttaac
    15421 ccaagtaatc ctgggtatat gatgagtgta agcggaagtt ccattgggga aaatgtcttc
    15481 gacgttatcc aaatttacaa tggagctgga cttactggca acttgctttg ggaaggcagt
    15541 cctggattgg gctctattcc aaccattaca tctacgactg ggccgttaac ggtaaaattc
    15601 acctcgaatg ccagcactac aggaaacgga tttgctttga atcttatttg tacccctccg
    15661 actactttca ccgttccaac atcaggagtt aataactaca cagtttgctc gggtgtgcta
    15721 tacgattcag gcggcccttt ggggaattac agcgacgatt caaatgggta tacgacactc
    15781 tatccaagta atccgggaaa catggtacaa atcggtggta cgttcactgg ccagaattgc
    15841 tgcgatttca tgcagatttt caatggcgta ggattgggtg gaaatttact ttggtctgga
    15901 aatccttctg ttggcgttgt tccaaccata acatccacgt cagggccatt aactgtgcag
    15961 tttatctcca atggtagtgt cgttggagca ggcttcgcct tagatataat ttgtttgcct
    16021 ccaataattt tcactgtacc gtcatcaggc aataatgaat acacggtatg ttccggaatt
    16081 ttatacgatt cgggaggacc tactggctcg tatgtcaata attctaatgg atatacaatc
    16141 ctcaacccaa gcaatccggg ttatatgatg acggtaagcg gtggttccat tggggaaaat
    16201 gtattcgacg ttatccaaat ttataatgga gccgggctta caggcaactt gctttgggag
    16261 ggcagtccag gtttgggctc tattccaacc attacatcta cggctgggcc attaacggta
    16321 aaattcacct cgaatgccag cactacagga aacggatttg ctttggatct tatttgtacc
    16381 cctccgacta ctttcaccgt tccaacatca ggagttaata actacacagt ttgctcgggt
    16441 gtgctatacg attcaggcgg ccctttgggg aattacagcg acgattcaaa tgggtatacg
    16501 acactctatc caagtaatcc gggaaacatg gtacaaatcg gtggtacgtt cactggccag
    16561 aattgctgcg atttcatgca gattttcaat ggcgtaggat tgggtggaaa tttactttgg
    16621 tctggaaatc cttctgttgg cgttgttcca accataacat ccacgtcagg gccattaact
    16681 gtgcagttta tctccaatgg tagtgtcgtt ggagcaggct tcgccttaga tataatttgt
    16741 ttgcctccaa taattttcac tgtaccgtca tcaggcaata atgaatacac ggtatgttcc
    16801 ggaattttat acgattcggg aggacctact ggctcgtatg tcaataattc taatggatat
    16861 acaatcctca acccaagcaa tccgggttat atgatgacgg taagcggtgg ttccattggg
    16921 gaaaatgtat tcgacgttat ccaaatttat aatggagccg ggcttacagg caacttgctt
    16981 tgggagggca gtccaggttt gggctctatt ccaaccatta catctacggc tgggccatta
    17041 acggtaaaat tcacctcgaa tgccagcact acaggaaacg gatttgcttt ggatcttatt
    17101 tgtactcctc cgaccacatt caccgttcca acatcaggag tcaataacta cacagtttgc
    17161 tcgggtactt tatatgattc aggtggccct tttgggaatt acagtgatga ctccaacgga
    17221 tatacagtac tgaatccaag taatccggga agtttgatgc aaatcggtgg ctcatttaac
    17281 ggacagaact gctgcgactt tttaaacata ttcaatggtg taggcttagg tggtaattta
    17341 ctttggtctg gaaatgcttc cgctggtgtc gttccaacca taacatccac g
//
LOCUS       DAOOQW010000032        17054 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_4087_length_17054_cov_5.794047, whole genome shotgun sequence.
ACCESSION   DAOOQW010000032 DAOOQW010000000
VERSION     DAOOQW010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 17054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17054
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4087_length_17054_cov_5.794047"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1793)
                     /locus_tag="PK281_03535"
     CDS             complement(<1..1793)
                     /locus_tag="PK281_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HOY48445.1"
                     /translation="MKKIFLLSIFFTVFHIVGVSQQFDWIKTVYASYPSFFPYNQGKD
                     IVVDSIGNVYTTGYFYYHPGDVPVDFDPGPGVFNFNRTGTYIQKLDRNGNFIWAKNFP
                     ESLSGGGSNIEDIAIDKAGNIYAAGAFSGTADLDPGPATLNYTANIGSDLFVVKLNSL
                     GEFQWVKIMPYTAGTYFPYVSDLTIDQQNNILISGNIHGEIDFDPSANTALFLAPNNE
                     GFVVKLRSNGDYLWARQLGFTDLSEVTAMETDNLGNVYLSGNTAAMDIDNGPETLFVN
                     TFGMGDSYVMKLDMNGDIIWVKNFGNSTNIDNPNDLTIDKLGNIYATGYFKGTAYFPN
                     TSDTLSIISTGNSNVFVTKVNTNGEFIWAKAFNGNDTIIGEEGRCIGVDSIGNVYVLG
                     SFKDSIDVDPGPAIAWITAQSFYNFSRSIFICKLDNDGNFIRAKATNGGGDVLAKAMV
                     IDNDANILFTGAMWTWFAGPIDVDPDTSIYEVEETYIDPIYVAKWKQCNGGSSTINES
                     ACENYTWNNQTYFESGSYTKSYTTSFGCDSIARLNLVILNKSVSQNIQICSGETFNIG
                     SQTLNQSGIYQTVFPASNGCDSLVTTHLTVDS"
     gene            complement(1804..4209)
                     /locus_tag="PK281_03540"
     CDS             complement(1804..4209)
                     /locus_tag="PK281_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48446.1"
                     /translation="MLKKFTCNNSFRFIRVFVFSILHWICNHPIILSAQAPQCHWVTR
                     ITGDDVQSFTALQVNSKGNVYAAGFAYLTTQIESFCDTSENFSFNNFNSTLVKLSVAG
                     DPIWRKDFNQGGGYNAIRKILLDKNDNIYITGNCYGPVDFNLDENANSNLTPNGSSDL
                     FLIKYDSLGNYQWGRRFGGISNFDISYLIDFSPDSNIIISGTFGDQVDFGNPGNPLVF
                     TSTGEQDNFTAKINSLDGSTLWAKQVGGISFDDVISQTIDQFGNIYLVGLYMFNQTDV
                     DPSASVQLTEPINDLPMRSYILKLSNQGNFIWVKQYEGFNTSTLSDIQIGPDNSLYTY
                     GVFSIDADYDFSPTNVVTAPDSSMGTFLAKYDLEGNFNWVKSIGSSGYGGATGWEKLV
                     IGNSGNIYVSGFFSGTNDFDPGPNQEIISAPDSASFNPFIACFSPVGDFQWVKTIRNP
                     VLAWGFGLAVDSLEKNFYLPGEFPNVSEFELGNNQFTYDGGFYDGYIAKFSFCERILN
                     KAYVETCEPFYEINGQQLTNSGSYTLNNRSPEGCDSVIILTLNLNDAHSEIAIQSCQA
                     VEVNGQFYESSGTYQQVIENGTPCDSVLIIQVKISEPNANIVTSNSCEPVSFNGITYN
                     QSGQYTQVFQNVAGCDSTVTFDIDVLQLNAQIFQTDSILFVNGTPTSIQWFDCITGQS
                     IPGANEPVFIPQQTGNYGAIVTVGECADTSNCRLIPVPPIAKVPPALCDNLIVSPNPV
                     HDFISFNLDKDSYLIRLFTATGALVWQKTGNPEEQEISFQHLAPAVYVLEIDQCRYKI
                     VKQ"
     gene            4351..4950
                     /locus_tag="PK281_03545"
     CDS             4351..4950
                     /locus_tag="PK281_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48447.1"
                     /translation="MQVAFVSECHSFYRIISDSLSKKYPQLNFVHYEHPDLFIEKPLP
                     NFTHFILKLTDRDSFDLKIKKIKTKDSHPITIGLVIKREFKTLKSVSPYFDYIFNDSE
                     IEEKLNIYFMNLLQAEDKINDTSKLEIETNKRKYVQLNTKLSRCLVLIAQNKTAAEIA
                     EDLAKSERTVEKYIAFLKNYFGVSKKKDLVDVVIDIAKF"
     gene            complement(4954..6174)
                     /locus_tag="PK281_03550"
     CDS             complement(4954..6174)
                     /locus_tag="PK281_03550"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOY48448.1"
                     /translation="MKKLLIIGTVWPEPTSSAAGTRMMQLIDWFQQAHWEICFACPAA
                     ESEFSVSLSDRNIRKEIIELNEDSFDEFVSEFNPDAVLFDRFMMEEQFGWRVAQNCPD
                     ALRILDTEDLHFLRIARQEAVKEGRTMELADLKNETAKREIAALYRCDLSLIISSYEY
                     ELLQSTFHLDASLLYYLPMQAGKIPVGNKSFEERSGFVTIGNYRHPPNSDSVKYLKTE
                     IWPLIRQALPKVEMHVYGSYPSPAMLQLNHPASGFYVHGRANEVDEVMQNARVCLAPL
                     RFGAGIKGKLIDAMRCQTPSVTTQIGAEGMHDGLEWNGFVEDDPKVFAEAAIELYSNE
                     NVWNEASKQGRFLIENSYQSNEKALGFLTRINELIETRNEHRNQNFIGAMLMHHTLQS
                     TKYLSKWIQEKNRK"
     gene            complement(6999..8501)
                     /locus_tag="PK281_03555"
     CDS             complement(6999..8501)
                     /locus_tag="PK281_03555"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY48449.1"
                     /translation="MKKILILLLLVVSSFSSNAQLFDFAHTAYFETTDQEPDMGTLLT
                     PSGNTLLNFNFNNAPIDHPLFNNGVYFPIYDCANHNLWLRVKHIAADPNGWGNGFSIE
                     QSMNSPFLPAGSADRIGGWNGFLYDFQIFSDVSLDGNRMNELGAPYPVQIIVESLETL
                     YNDGGSQFEWLSFEIFNPESDGWQLMATNFTGINPLSNPGFSAELNYSYPVDWGMAPE
                     GFSTDFPNGSNTIYAVDLNLSSAYHSEFRMSAGAVSHFRYGYEFNTGGYQGMSMTFGG
                     APTIIGSITPQCGTDANGIISVSTTGPSPFVFDWGNGITGTLLDGLTSGTYNVTLTDG
                     SGCISQASFDILEAIPVTATLEVIPSEDGVSLIITPEGGSGEYTFEWSTGSFEDSIFV
                     NSLGIYEFIVSSNNGCSFSASYEFLGTEAVNSPHISLYPNPVGNFSSISGSPNTHYRV
                     LGLDGRFIQSGNLDALGKGMIATSTWSKGLYLLQLIEKSGATYSMRFVVE"
     gene            complement(8523..9995)
                     /locus_tag="PK281_03560"
     CDS             complement(8523..9995)
                     /locus_tag="PK281_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48450.1"
                     /translation="MTFKTTLRMVAAVVVSLLLLASCKKEKEPELVNEKPNIPVVPAD
                     GGLYVSAPGNPTGSSSTTTIGIAGGTAVSTDGRLTLSIPAGALSGETQIGIQPISNLA
                     PLGIQSSAYRLTPEGVTFNSPVELTFSYANLELEGTPADFLWVTTQKADGSWEAARKS
                     VVNTSNQTVRVEVSHFSDWALGKFIDIGLAPGTATLAKNQILRLFAVGFKQVQDDDDD
                     FVPLYPINHDDGELVPLVPVMPDETQMTGFQITGWSLNGNMAPVSGDAGHLVPEDFTA
                     VYQAPAEVPSPNPVAVSIHLKANNYLGGISNYILTSNITILDNDLYLHLNAPGDDYTY
                     YQWGINGSIPPSTGEVCMINCGFSEGQLEIAGGVTSSQGAYSHSFVMEVGGLQPGTYV
                     LMGSNCNGDEGVSYSADPMSGFAYSLNLVRRNMNNQVCEDEYTCADFITKITAYNDET
                     KIVEGNFSGNLYYDPPDAWDNCTSSEEIQVSGSFRLKVLF"
     gene            10119..13214
                     /locus_tag="PK281_03565"
     CDS             10119..13214
                     /locus_tag="PK281_03565"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HOY48451.1"
                     /translation="MKPDVKNKLFGLLHFLFALTLSFEVHAQILNKSLPFFSGKNTQN
                     GFLAYEVNSIASDSLGMVWMATNLGLYRYDGIHFVDFTHLLANQKQTQSNYISAVFTD
                     SRKNIWVSHENALVCINGINQYVRNFIHNPRKSESAPRGSAFRIREDQHGNIWTCNGT
                     SELSCLNTRSGIFNHFKVKSELNASPLEVSIRDFWFSSDTTYWLATRAEMIRFNPKTG
                     LTKAYTVLPGKGKRNPGLITRMCPDPSSPEIIWLGTWGKGLLKFNTSTGNTKQFLYEI
                     SLAENISNILFDIISRNEHILWAGGNGLVEFDTRTENFRIYKHDPLRKSSLNVNEIRS
                     LYRDPNGRFWLGTVDGFSLFAPEQRQITLHTLPFKTSIGQFIYYTNSEEIYFVNYFSD
                     RKLYRLNRDLEITGSWPIPGADEHFSEPFGFCHDPDGRFWIATTHSGLWCFDPKTKQM
                     HAITAKQGDLNVLDLNSTVMRCDKNGILWIGTMSSGLLRCDTRNPVIETIPEIRNRVD
                     GLTFSSDGSLWIQEGRFKLHRFDTKTYQIISYGSENRTNESQIRSISDVCQGPQGKMF
                     ISTLKNGILEIQNGQINKVSGLELLQNIHSIYADSSANLWMLSSNRLIRYDRVSRKTQ
                     NFSTENGLSGNLGYSHFIPVSNSEVWFQTEDGIFVIQTDFIPTTKSNYQLILNSIKLF
                     DTEIAGAGKISTKQGIQLNHTENYLTFEFQAIAFHQQEDLQYAYMLEGLDPNWVESGN
                     RHFASYQALPPGNYRFRVKVSDGVNGWETEELILPVEIIPAWWQTWAFRIVLSILFIG
                     LIGLLIRYYATRKLRKRLQRFEQLQEIEQIRNRIARDIHDEIGSGLSKIALLSGGLER
                     KAKGDLELVKTSQRVRQLSREVIRSMGEIIWAVNPGNDEVSSLISYVRSYLNQFSEES
                     NIRVHSELITENPEYLQSRLRPELKRNLLMILKESLNNILKHANASEVDVHLEFRNKH
                     ILLLIRDNGNGIKSTPDFRHGNGMKNMQKRATELNGNLDIQESDGLRILLEFPWNPQQ
                     S"
     gene            13290..13916
                     /locus_tag="PK281_03570"
     CDS             13290..13916
                     /locus_tag="PK281_03570"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOY48452.1"
                     /translation="MPIRIAVVEDDQELRIHFTEMLQEVSDFECIGAFANAEDFESSF
                     ADLNPDVVMMDINLPGKSGIESISRCKPHKPDVQYLVCTVFEDSDNLFKALLAGATGY
                     ILKISDLDVMEKAIRDIHAGGSPMSPQIARYVINALPLKKQNSSILESLSKREREILE
                     LLSEGYRYKEIADKLFLGVDTVRTYIRNIYSTLQVHSRTEALNKAFGK"
     gene            14144..14407
                     /locus_tag="PK281_03575"
     CDS             14144..14407
                     /locus_tag="PK281_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48453.1"
                     /translation="MSHVFVRLRGIQLEDLEMVLMADAAEHAERGMNLEQMWHNADDA
                     EEVLFLFSAEDLDKARAYINEMLGQAMSAFSDLPSPSITYLQN"
     gene            14407..14934
                     /locus_tag="PK281_03580"
     CDS             14407..14934
                     /locus_tag="PK281_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48454.1"
                     /translation="MLAQFQLDALLADTFLSTKEKTETISRALLEAQLEVDSLIAFAA
                     NSKDPVKATCIEAFEFATNQVPGIANVALLEFVSKELGAKAPRVKWESAKVIAHIAHL
                     FPANLSDSVKGLLVNTEHSGTVVRWSAATALGEILKLNLAKHKDLKVALESICEREEK
                     NSIKKIYQAAFKKSN"
     gene            14975..15856
                     /locus_tag="PK281_03585"
     CDS             14975..15856
                     /locus_tag="PK281_03585"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HOY48455.1"
                     /translation="MKTTTPFIAIGLIFIGLSAFKPKAVVKSLPKSVQKEFVFIPSSD
                     ETQSGLGKPMSAFMMSKTEISNGQYRAFLADLKSKGELDKYALAVYDSTVWKVSYCEP
                     FMNYYGWHPAYRNYPVVGVSKAGAELYCQWLTEKINTQLAGKSKVKVLIPNSEQWLYA
                     AYSGTKGTVYSWGGNKLQNLKGQHLCNYRNLGAENIHWNEVNNQFEIERTTSLGVVSG
                     ISDNADITAPVTSYFETSYGLYNMNGNVAELVSDGDKAMGGSWNSVGYDVRNESFVSF
                     QKPTSTVGFRPVIIWEE"
     gene            15943..16344
                     /locus_tag="PK281_03590"
     CDS             15943..16344
                     /locus_tag="PK281_03590"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HOY48456.1"
                     /translation="MNRKEFLSILGISAGGLVVATCLGSCKKTETNPVDFTIDLSNSS
                     YSALNTNGNFLVTKGVIIAKTNTGSFIAVAAACTHEGTNVTYQAANNRFHCNNHGANF
                     SSNGAVQNGPANTALQQYNTELTGTTLRIYS"
     gene            16436..16861
                     /locus_tag="PK281_03595"
     CDS             16436..16861
                     /locus_tag="PK281_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019387757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOY48457.1"
                     /translation="MNQRISFITLGVKNLQIMRTFYTQVFGWTLLKDSDGIVFFKLNG
                     FILGLFPSHELAEDANVSEIGSGFKSFSLSINLNSEKEVDLLFQELISKGARSLKSPE
                     RVFWGGYRGYIADPEDNLWELAYNPFLKMDEKGNVVHHP"
BASE COUNT         5495 a         3372 c         3008 g         5179 t
ORIGIN      
        1 gaatccacag ttaaatgggt agtcaccaaa ctatcgcacc catttgaagc aggaaatact
       61 gtttgataaa tacctgattg atttaatgtt tggctgccaa tattgaatgt ctcacccgaa
      121 caaatttgaa tattttgaga aacgcttttg tttaaaatga ctagattaag tctagcgata
      181 ctatcacagc caaaggatgt ggtataagat ttagtgtaag aaccactttc aaaatacgtt
      241 tgattgttcc aagtgtaatt ttcacaagcc gattcattaa tggttgaaga acctccgtta
      301 cattgtttcc atttggcgac ataaattgga tctatatatg tttcttccac ttcatatatg
      361 gaagtatctg ggtcaacatc tataggccct gcaaaccatg tccacatagc acctgtaaaa
      421 agaatattcg catcattatc aattaccata gctttagcta aaacatcgcc accaccattc
      481 gttgctttag ctcttatgaa gttaccatcg ttatctagct tacagataaa aattgaccga
      541 gagaaattat aaaaagattg tgcagtaatc cacgcaattg caggtcccgg atctacatca
      601 atactatcct taaaagatcc caatacataa acatttccaa tactatcaac accaatacac
      661 cttccttctt caccaattat tgtatcattt ccattgaatg ccttagccca aataaattcg
      721 ccgttagtat tcacttttgt aacaaagaca tttgaatttc ctgttgatat tatagataaa
      781 gtatctgatg tatttggaaa atatgctgtt cctttaaaat aacctgttgc ataaatatta
      841 cctagtttat cgatggttag atcatttgga ttatcaatgt tagtgctgtt gccaaaattt
      901 ttgacccaaa taatatcccc attcatatct aatttcatca catagctatc acccatacca
      961 aatgtattga caaacagtgt ttctggacca ttatcaatat ccattgcagc agtattacct
     1021 gaaagatata cattccccaa attatccgtt tccatggctg taacttcaga aagatcagta
     1081 aaacctaatt gtcgagccca taaataatcg ccattacttc ttaatttaac aacaaatcct
     1141 tcattatttg gggccaagaa aagtgcagta tttgcactag gatcaaaatc aatttctcca
     1201 tgaatattcc cactaattaa aatattattt tgctgatcaa tagtaagatc actcacatat
     1261 ggaaagtaag ttcctgctgt gtaaggcatt attttaaccc attgaaattc ccctaatgaa
     1321 tttaatttca ctacaaaaag atcactacca atattagctg tatagttgag tgttgcaggt
     1381 cccggatcta aatctgctgt gccagaaaaa gctcctgcag cataaatatt cccagcttta
     1441 tcgatggcaa tatcttcaat gttagaacct cctccactca gtgattctgg aaagtttttc
     1501 gcccaaatga aatttccgtt tcgatccagt ttttgaatat atgttccggt tcgattaaaa
     1561 ttaaaaacac ctggccctgg atcaaaatca acaggaacat ctccaggatg ataataaaaa
     1621 taacctgtag tatagacatt tccaattgag tctacgacaa tatcttttcc ttgattatat
     1681 ggaaaaaaac ttggataact cgcataaacg gttttaatcc agtcaaattg ctgtgaaaca
     1741 cctactatgt gaaataccgt aaaaaagata gataaaagaa aaatcttttt cataattcag
     1801 attttattgt tttacaattt tatacctgca ctgatcaatt tcgaggacat atactgctgg
     1861 agcaagatgc tgaaatgaga tttcttgttc ttctggattt cctgtttttt gccacacaag
     1921 tgcaccagta gccgtaaaca agcgaatgag gtaggaatct ttatctaaat tgaacgatat
     1981 gaaatcatga accggattag gtgaaacaat taaattatca cacaaggcag gtggtacttt
     2041 tgcaatgggt ggaacaggaa tgagtctgca attgctggta tcggcacatt cgccaacggt
     2101 aacaatggct ccgtaattac ctgtttgttg tggtataaaa acgggctcat tggcacctgg
     2161 aattgactgt ccggtaatac aatcgaacca ttgaatactg gtaggagttc cattgacaaa
     2221 caggatagaa tcggtttgga aaatttgggc gttgagttga aggacatcga tatcgaacgt
     2281 tactgtacta tcgcagcctg ctacattttg aaatacttgt gtgtattgcc ccgactggtt
     2341 ataggtaatt ccattaaaac taactggctc acaggaattg gaagtaacaa tattggcatt
     2401 gggctctgaa attttaacct gaataattaa aacactatcg caaggagttc cattttcaat
     2461 tacttgctgg taggtaccag aactctcata aaattgaccg ttcacttcaa ccgcttggca
     2521 agattgaatt gcaatttcag agtgggcatc attcaaatta agtgtcaaaa taataacact
     2581 atcacaccct tcaggagaac gattattgag tgtatatgaa ccagaatttg ttagctgctg
     2641 tccgtttatt tcgtaaaaag gttcgcacgt ttcaacatag gctttattta aaattcgttc
     2701 acaaaaactg aatttggcaa tatacccatc ataaaaaccg ccatcatagg taaattgatt
     2761 attcccaagt tcaaactccg atacgtttgg aaactctcct ggcaaataaa aattcttttc
     2821 caaagaatcc acagccaaac caaaacccca agctaaaaca ggatttcgaa ttgttttcac
     2881 ccactgaaaa tcaccaacag ggctaaaaca ggcaataaat ggattaaatg aagccgaatc
     2941 gggtgcagaa ataatttcct gattgggacc aggatcaaaa tcatttgtgc cactaaaaaa
     3001 tccagataca taaatatttc ctgaattccc aataactaat ttctcccatc cagttgctcc
     3061 accataacca gaagaaccga tagattttac ccaattaaaa ttgccttcta aatcatattt
     3121 agccaagaat gttcccattg aactatcagg cgcagtaact acattggtag gagagaaatc
     3181 ataatcagca tctattgaaa acacaccata ggtatataaa gaattatcgg ggccaatttg
     3241 gatatcgctc aatgttgatg tattgaatcc ttcgtattgt tttacccaaa taaaatttcc
     3301 ttgattagat aatttcaaaa tataggaacg catgggtaaa tcattaatag gttcggttaa
     3361 ttgaacacta gcgctcggat ctacatcagt ttgattgaac atatataatc caactaggta
     3421 tatgtttccg aattgatcga tggtttgaga aatcacatcg tcaaaactaa ttccaccaac
     3481 ttgtttagcc caaagtgtac tgccatcaag tgaattgatt ttagcagtga aattatcttg
     3541 ttctccagtt gaagtaaata caagtggatt tccgggattt ccaaaatcaa cttgatctcc
     3601 aaatgtcccg ctaatgatga tgttgctatc aggagaaaaa tcaattaaat aacttatatc
     3661 aaagttagat attcctccaa accttcttcc ccattgataa tttccaagtg aatcatattt
     3721 gattaggaac aaatcacttg atccattagg agttaaatta gaattcgcat tttcgtctaa
     3781 attaaaatct actggtccat agcaattgcc ggtgatatag atgttatcgt ttttgtcaag
     3841 aagtatttta cgtattgcat tatatcctcc tccttgatta aaatcttttc tccaaatggg
     3901 atcacccgca acacttaatt ttacaagagt tgagttgaaa ttattaaagc tgaagttttc
     3961 agatgtatca caaaaggact cgatttgagt tgttaaatat gcaaaaccag ctgcgtatac
     4021 atttcctttc gaattgactt gtaatgcagt aaaactctga acatcatctc cagtaatccg
     4081 tgtaacccaa tggcattgag gagcttgtgc cgaaagaata atagggtggt tacatatcca
     4141 atgtaatatg ctaaaaacaa atactctaat gaatctgaaa gaattattac aagtaaattt
     4201 cttcaacata taaatcggat ttattgaacc ttatcaaatt tatccctttc aataagccac
     4261 ttgaattatg tcccacaatt gggtattttt ttgggtagtt ttaatttatg tcggaaaaga
     4321 agttaaattt gaacttctac cagtgtcatt atgcaagtcg cctttgtatc tgaatgtcat
     4381 tctttttacc gaattatttc agacagttta tccaaaaaat acccccaatt aaattttgtt
     4441 cattacgaac atcctgatct ttttatcgag aaaccgcttc cgaacttcac tcattttatt
     4501 ttgaaattga cagacagaga ttccttcgat ttaaaaatta aaaagataaa aacgaaggat
     4561 agtcatccta ttacaattgg ccttgttatt aaacgagaat ttaaaacact aaaatcagtc
     4621 agcccctatt ttgattatat ttttaatgac tcggaaatag aagaaaaact aaatatttat
     4681 tttatgaatc tgctacaagc tgaggataaa ataaatgata ccagcaaact agagattgaa
     4741 acaaataaac ggaaatacgt tcaattaaac actaaattaa gtcgttgttt agtgctcatt
     4801 gctcaaaata aaacagctgc tgaaattgct gaagaccttg ctaaatcaga aagaacagta
     4861 gaaaaataca ttgcatttct aaaaaactac tttggtgttt ctaagaaaaa agatttagtg
     4921 gatgtagtaa ttgatattgc taaattttaa tctttatttt ctattttttt cctgtatcca
     4981 tttcgataaa tacttcgtac tctgtagcgt atgatgcatc aacattgcac caataaaatt
     5041 ctgattacga tgctcatttc gtgtttcaat taattcattt attcgagtta aaaatccaag
     5101 tgctttttca tttgattgat agctattttc tatcaagaac cgtccttgtt tactcgcttc
     5161 attccacacg ttctcgttcg agtagagttc aatagctgct tctgcaaata ctttaggatc
     5221 gtcctccaca aacccattcc actccagtcc atcatgcatt ccctctgctc ctatttgtgt
     5281 ggtaacggat ggtgtttgac aacgcatcgc atcaatcaat ttacccttta ttcctgcacc
     5341 aaaacgtaaa ggagccaaac aaactcgagc attctgcatc acttcatcta cctcgtttgc
     5401 tcgtccatgc acataaaatc ccgatgcagg atgattcaat tgcaacatgg caggcgatgg
     5461 ataagaacca tacacatgca tttcgacctt gggtaaagct tgacgaataa gtggccagat
     5521 ttccgttttt aaatacttca cagaatcgga gttgggcggg tgtcgataat tcccaatcgt
     5581 tacaaatccc gagcgttctt caaatgattt attaccaact ggaatttttc ccgcttgcat
     5641 aggtaaatag tacaaaagcg aagcatcaag atgaaatgtt gattgcaaca attcgtactc
     5701 gtaggaagaa ataatcaaac tcaaatcgca gcgatacaaa gctgcaattt cccgtttagc
     5761 agtttcgttt ttcaaatctg ccaactccat cgttcgccct tccttcactg cttcctgacg
     5821 agcaatacga agaaaatgta aatcttcagt atctaatatc cgcaaggcat ctggacaatt
     5881 ttgcgctact cgccaaccaa attgctcctc catcataaaa cggtcgaaaa gcaccgcatc
     5941 tggattgaac tctgaaacaa attcatcaaa actatcctcg ttcaattcga ttatttcttt
     6001 ccgaatgttg cgatcactca acgatacaga aaattcactt tccgcagctg gacaagcaaa
     6061 gcaaatctcc caatgagcct gctgaaacca atctattaac tgcatcatac gtgtcccagc
     6121 tgccgacgaa gtaggctcag gccaaacagt accaatgatg agcagttttt tcatatcgcg
     6181 aaactaagac gaaaatcacg aatcctgaaa gtaaggttac aacttggcgt tagtgcgtgc
     6241 ccattgcatg ccttcgccaa ggctatggca tactatggtc gcgtcatcgc ctcatagtcc
     6301 ttttaggtgc catagccttg gcgaaggcac ccaaaacggc tagttccggc tctaccgctc
     6361 acgcgttttg ctaaaagctg tcatcttcaa taataataga cattcgagaa ttgaaaaaag
     6421 aaatcaacag ctctgtcaaa aactagccgt ttataaacgt ttactatacg actcatgtgc
     6481 gccgaatttc gtttctttca gctggagggt ggttagaggt ggcccccgcg tacgcgagaa
     6541 aatttagaag tattatattg attgtgtggc atttctcacg aaaaataaaa tgaacctcaa
     6601 tatagcattt gattccagat tcactttcta tccaatttat caggtgatca acctacttca
     6661 gggtaagtga aaatgaaata acatttccgc tcgaaaattt tacacgaaac aaatagctta
     6721 gttcaattga aaaatgcagg aatattcttt cttcaattct cacatgctgg tcaatcgaac
     6781 tactttttag taattattta attgatttta ttcaattaat aataatcctg ataaaagaaa
     6841 ttacatgaag gaataagctt agtaacacat cgatgtagga gtcttttcga cgaacaattt
     6901 ttctttaaat aaaaaatgga ttccaactta ttttaaattg aaatccattt aaactggttc
     6961 gcctattctt aatacaattc agaatcgcat tctatccatc attcaactac aaatcgcata
     7021 ctgtaagttg cccccgattt ctcgattagt tgtaataaat ataaaccttt gctccaagta
     7081 gaggtagcaa tcattccctt tcctaacgca tcaaggtttc cactttgaat aaagcgccca
     7141 tcaagcccaa gaacccgata gtgagtattt ggacttcctg agatactact gaaattgcct
     7201 acaggatttg gataaagcga aatatgtgga gaattgactg cttcagttcc taaaaactca
     7261 tatgaagctg aaaatgagca accattgtta ctactaacta taaattcata tatccctaaa
     7321 ctattaacaa aaatgctgtc ttcaaaactt ccagtactcc attcgaacgt gtattctcca
     7381 ctgccacctt ctggagtaat gataagtgaa actccatctt cactcggaat aacttccaat
     7441 gttgcagtta caggaatggc ctctaaaata tcgaaacttg cttgtgaaat acaccccgaa
     7501 ccatcagtta gagttacgtt atatgttcct gaagttaacc catcgagcaa tgtgcctgta
     7561 atgccattac cccagtcgaa aacaaaagga ctaggacctg ttgtcgaaac agaaataatt
     7621 ccattggcat cggtcccaca ttgaggtgtt attgaaccta taattgtagg tgccccaccg
     7681 aatgtcatac tcataccttg ataacctccc gtattaaact cataaccata ccgaaaatga
     7741 gaaaccgctc cagccgacat cctgaattcg gagtggtaag ccgaagataa attcaaatcg
     7801 acagcatata ttgtattgct accatttgga aaatcggtcg aaaatccctc gggagccata
     7861 ccccaatcaa ctggatatga atagttcagt tcagcactaa atcccggatt actcaatgga
     7921 tttatccctg taaaattggt tgccatcaat tgccagccat cgctttcggg gttaaagatt
     7981 tcaaatgaca accattcgaa ttgtgaacca ccatcgttgt agagtgtttc gagactttcc
     8041 acaataattt gaaccggata aggtgcacct aattcattca ttcgatttcc atcgagactt
     8101 acatccgaaa aaatctggaa gtcatataaa aatccattcc accctccaat acgatcagca
     8161 gaaccagctg gtagaaaagg actattcatc gattgttcta tactaaatcc attcccccat
     8221 ccattgggat ctgcagcaat atgtttaacc cttaaccaca aattgtgatt agcacaatcg
     8281 taaattggaa aatacacccc gttgttaaat aatggatgat cgattggagc attattaaag
     8341 ttaaaattga gcaatgtgtt tccacttggt gtgagcagtg tacccatatc tggctcttga
     8401 tcagttgtct caaaatatgc cgtatgagca aaatcaaaaa gttgagcatt agatgagaac
     8461 gaactcacta ctagcaataa aagaattaaa atctttttca tgattgtgat tttaaattgg
     8521 aattagaaaa gtactttgag acggaatgag ccacttactt gaatttcttc tgaactagtg
     8581 caattatccc aagcatctgg tggatcataa tacaaattgc cactgaaatt accttctacg
     8641 atctttgttt catcattgta tgccgttatt ttcgtgataa aatcggcaca agtatattca
     8701 tcttcacaaa cttgattatt catatttcgc cttaccagat tcaatgaata agcaaaacca
     8761 gacattggat cagcactata agaaactcct tcatctccgt tgcaattcga ccccattaat
     8821 acataagtac ccggttgtaa tccacccact tccatcacaa aactgtgaga ataggctcct
     8881 tgcgaacttg ttacaccacc agctatttcg agttgacctt cactaaaacc gcaatttatc
     8941 atacacactt ctccagtact gggtggaatt gaaccattta taccccattg ataataagta
     9001 taatcgtctc caggagcatt caaatgaaga tacaaatcat tatctagtat agtaatgttt
     9061 gaggtcaaga tgtagtttga aattccccct aggtaattat ttgctttcaa gtgtatggat
     9121 acagctactg gattaggaga tggcacctca gctggcgctt ggtacacagc tgtaaaatct
     9181 tccggaacta aatgtcctgc atctcccgaa actggtgcca tattgccatt tagcgaccaa
     9241 cctgtaattt gaaatccggt catttgggtt tcgtctggca tcactggaac taaaggaact
     9301 aattcgccat cgtcgtgatt gattggataa agtggaacaa aatcatcatc atcgtcctga
     9361 acctgtttaa aaccaactgc aaacaaacgt agaatctgat tcttcgctag tgtagcagtt
     9421 ccgggagcaa gtcctatgtc aatgaatttc cccaatgccc aatcactgaa atgtgatact
     9481 tcaacacgaa ccgtttgatt actcgtattc acaacacttt tccttgctgc ttcccatgaa
     9541 ccatcggctt tttgtgtagt aacccataag aaatcggcgg gcgttccttc taactcaagg
     9601 ttagcataac tgaaagtaag ctcaacaggt gaattaaatg ttactccttc gggtgttagt
     9661 cgataagcag aactttgaat tccaagcgga gcgagattac ttataggctg aataccaatt
     9721 tgagtctctc ccgataaagc ccctgcagga atactaagtg ttaaccgacc gtctgtagaa
     9781 actgctgtac caccagcaat tccaatagtt gtcgtacttg atgagcctgt tggatttcca
     9841 ggtgcactta catacaatcc accatcagct ggaactactg gtatattagg tttttcattt
     9901 actaattcag gttctttttc ttttttgcaa gacgctagca gtaatagcga aactaccaca
     9961 gctgctacca ttcggagtgt cgttttgaac gtcattattc agtagatttt caacacgaca
    10021 aagattcttc aggtaagcgg cctacacaat cgcataaaag taagtttgtt tatgtatcga
    10081 ttagttgagc cgtaattctt gtgtaagttg cattcgaaat gaaacccgac gtaaaaaata
    10141 aactcttcgg ccttttacac ttcctttttg cactgactct atcgtttgag gttcatgcgc
    10201 aaatcctaaa taaaagcctg ccctttttta gtggaaaaaa cacgcaaaat ggttttctag
    10261 cttatgaggt gaatagcatc gcatcagaca gccttggtat ggtttggatg gcgacgaatc
    10321 tgggacttta tcgatacgat ggaattcact ttgttgattt tactcattta ctagctaatc
    10381 aaaaacagac acaatcaaat tacatttctg ctgtatttac tgactcccgg aaaaacatct
    10441 gggtttcaca tgaaaatgca cttgtttgca tcaatggaat taaccaatac gttagaaatt
    10501 tcatccataa tcctagaaaa tctgaatcag caccccgagg aagtgcattt agaattcggg
    10561 aagaccagca tggaaatatt tggacatgta atggaacttc agaattatcc tgtttaaata
    10621 cacgttcagg aatatttaat cacttcaaag taaaatccga attaaatgct tctccacttg
    10681 aagtgagtat tcgtgatttt tggttttctt ccgacaccac gtattggctc gctactcgcg
    10741 cagaaatgat ccggtttaat cctaaaacag gactaacaaa agcttataca gttttaccgg
    10801 gtaaagggaa acgtaatcct ggtttgatta ctcgaatgtg tcctgatcca agttcacctg
    10861 aaattatatg gcttggtacc tgggggaaag gtttactcaa attcaataca tcaacaggaa
    10921 ataccaagca attcttatat gaaataagcc ttgcggaaaa tatttcaaat attctttttg
    10981 atattatttc acgaaatgaa catatccttt gggctggtgg aaatggttta gtcgagtttg
    11041 atacacgtac cgaaaatttc cgcatttaca aacatgaccc actgaggaag agcagtttaa
    11101 atgtgaatga aatcagaagt ctttatcgtg atcccaatgg aagattttgg ctaggtactg
    11161 ttgatggctt ttcgttgttt gcacctgaac aacgacaaat taccctgcat acacttccat
    11221 ttaaaacatc cattggtcag tttatttatt acaccaattc ggaagaaatt tacttcgtga
    11281 attatttctc tgatcgaaaa ctttatcggt taaacagaga tctcgaaatc actggctcat
    11341 ggcctatccc aggtgccgat gaacattttt ctgaaccttt tggattttgc catgatcctg
    11401 atggaagatt ttggatagcc actacccata gcggactctg gtgttttgat cccaaaacca
    11461 agcaaatgca tgctattact gcgaagcaag gtgatttgaa tgtactcgat ttaaattcta
    11521 ccgtgatgcg ttgcgataaa aatggcatac tttggattgg taccatgtcg agtggtttat
    11581 taaggtgtga tactcgaaat ccagtaattg aaactatacc cgaaatacga aatcgagtag
    11641 atggtttaac cttttcttcg gatggtagtt tgtggataca agaaggtcga ttcaaacttc
    11701 atcgtttcga cacaaaaaca taccaaatta tttcttacgg ctcagagaat cgtactaatg
    11761 agtctcaaat tagaagtatc tcagatgtat gtcaaggtcc acaaggaaag atgtttatat
    11821 caactttaaa aaatggtatt ttagagatac aaaatggtca aataaataaa gtttcggggc
    11881 ttgaacttct tcagaatatt cattccattt atgccgattc atctgccaat ctttggatgt
    11941 taagctccaa tcggttaatt cgatatgatc gagttagtcg aaaaacgcag aatttctcaa
    12001 ccgaaaacgg actttcagga aatctcggat attcccattt tattcctgtt tccaattcag
    12061 aagtttggtt ccagactgaa gatgggattt ttgtaattca aaccgatttt attcctacaa
    12121 caaaaagtaa ttatcagctt attttaaata gcattaaatt gtttgatacc gaaatagctg
    12181 gagcgggtaa aatatctact aaacaaggca tacaattaaa tcacacagag aattatttaa
    12241 ccttcgaatt tcaagcaatc gccttccatc agcaagaaga ccttcaatat gcatatatgc
    12301 ttgaaggatt agatcccaac tgggttgaat cgggaaatcg ccattttgca agttatcagg
    12361 cattgccgcc cggtaattac aggtttcgag ttaaagtctc agatggtgtt aatggctggg
    12421 aaactgaaga attaattcta cctgttgaaa taattcctgc ctggtggcaa acatgggcgt
    12481 tccggatagt attgagcata ctttttatcg ggttaatcgg actattaatt cgctattacg
    12541 ctactcggaa attacgtaaa cgcctccaac gattcgaaca acttcaggaa attgaacaaa
    12601 tccgaaaccg catcgctcgc gacattcatg atgaaattgg atccggactt tcgaaaattg
    12661 cactgcttag tggcggactc gaacgcaaag ccaaaggtga tctcgaatta gtaaaaacaa
    12721 gtcagcgagt tcgacagcta agtcgtgaag tcattcgcag tatgggagaa attatatggg
    12781 cggtaaatcc cggaaatgat gaagtttctt ccttaatatc ctatgtacgt agttatctga
    12841 atcaattttc agaagaaagt aacatccggg ttcattcgga actaatcacc gaaaatcctg
    12901 agtatttaca gtcacgatta aggcctgaac tcaagcggaa tttactgatg attttaaaag
    12961 aaagcctaaa caatattcta aaacacgcaa atgccagtga agtagatgtt caccttgaat
    13021 ttcgaaataa gcatattttg ttactgatac gcgacaatgg aaatggtata aaaagcacgc
    13081 ctgattttag acatggaaat ggtatgaaaa acatgcaaaa acgggcgact gaattaaacg
    13141 gcaatctaga cattcaagaa tcagacggat tacgaatctt gcttgaattt ccttggaacc
    13201 ctcaacaatc ataatcttac tattatgcga taccagagcc cattgaggta tttacctttg
    13261 tacttccgca taaataaaaa atctaaacta tgcccattcg tattgcagta gttgaagatg
    13321 atcaggagct tcgaattcat tttacagaaa tgctacaaga agtgtccgat tttgaatgca
    13381 tcggagcgtt tgccaatgcg gaggattttg aatcttcttt tgccgattta aaccccgatg
    13441 ttgttatgat ggatattaat cttcctggaa aatcgggtat tgaaagtatt tccagatgca
    13501 aaccgcataa acccgatgtt cagtatttag tttgtactgt tttcgaggat tccgacaatc
    13561 tatttaaggc acttttagcc ggagcaacag gttatatttt aaaaatatct gatttagatg
    13621 tcatggaaaa agcaatccgt gatatccatg caggaggatc acctatgtcg cctcagatcg
    13681 ctcgttatgt catcaatgca ttacccttaa aaaagcaaaa ttcgtctata ctagaatcac
    13741 ttagtaagcg cgaacgtgaa atccttgaac tcctctccga aggttaccgt tacaaggaaa
    13801 ttgctgataa attattttta ggtgttgata ccgttcggac atatatccgg aatatttatt
    13861 ctacccttca agtacattcc cgtactgagg ctctgaacaa agcatttggt aagtaaacca
    13921 acttaacaca actgcaatcg acacatttca ttatcagaat tgcatgatcc actctgcccc
    13981 attttcaatt aaatcttctt cattcttaaa gcatctctac caatagaaaa ctgaatcgtc
    14041 aagtccaaat tgctttattg tcgtgctttt actactcaac gaattaagta atcatttgac
    14101 catctaaagc tttttctatc tttcggactt taaccgatac gttatgtcac atgtgtttgt
    14161 ccgtttgcgc ggaattcaat tggaagattt ggaaatggtt ttaatggccg atgctgccga
    14221 acatgccgaa cgtggaatga acctagaaca aatgtggcac aatgccgatg atgctgaaga
    14281 agtattgttt ttattctcgg ctgaagatct agataaagca cgtgcttaca tcaatgaaat
    14341 gttaggtcaa gccatgagtg ctttttcaga tttgccatct ccttcaatta cctatttgca
    14401 aaactaatgc ttgcacaatt tcaacttgat gcgcttttgg cagatacctt tttgagtaca
    14461 aaagaaaaaa ccgaaaccat tagtcgcgct ttgttagaag ctcaattaga agtagattca
    14521 ttaattgctt ttgcagctaa ttcgaaagat cctgtaaaag ctacatgtat cgaagcattt
    14581 gagtttgcca caaatcaagt tcccggaata gcgaatgtgg cattattgga atttgtgagt
    14641 aaagaactag gagcaaaagc acctcgagtg aaatgggaaa gtgctaaagt cattgctcat
    14701 attgcccact tatttcctgc taatttatcg gattcagtta aaggcttgct agtcaatacc
    14761 gaacattccg gcactgttgt tcgctggagt gctgcaacag ccttaggaga aattttaaag
    14821 ttaaacctcg ctaaacacaa ggatttaaaa gttgctttgg aatcgatttg cgaacgagaa
    14881 gagaaaaata gcatcaaaaa aatctatcaa gcagcattca aaaaatcaaa ctagataaac
    14941 gcaatttctt gattcattaa ccgttaggat tttcatgaaa acaactactc catttatcgc
    15001 tatcggcttg atctttatcg gacttagtgc ctttaaacca aaagcagttg taaaatcgct
    15061 acctaaatcg gtacaaaagg aatttgtatt catcccttcg agtgatgaaa ctcaatcagg
    15121 tttaggaaaa cctatgtcgg catttatgat gtctaaaaca gagattagta acggtcagta
    15181 ccgagcattc ttagccgact tgaaatcgaa aggtgagctc gataaatacg ctttagcagt
    15241 atatgattca actgtctgga aagtcagtta ctgcgaacct ttcatgaatt attacggttg
    15301 gcatccagca tatcgaaatt atccagtagt tggcgtttcg aaagcaggtg ctgaactgta
    15361 ttgccaatgg ctaacggaaa aaatcaatac tcaattagca ggaaaaagca aggttaaagt
    15421 gttgattcca aacagcgaac aatggttgta tgcagcatat tctgggacaa aaggcacagt
    15481 atattcttgg ggtggaaata aactccaaaa cctaaaagga cagcatttat gcaattaccg
    15541 gaatctagga gctgagaaca ttcactggaa tgaagtcaat aatcagtttg aaatagagcg
    15601 tactaccagt ttgggagttg tgtcgggaat aagtgataac gccgatatta ctgctccagt
    15661 tacctcctat tttgaaacca gctacggttt atataacatg aatggaaatg tagctgaact
    15721 tgtgagtgat ggagataaag caatgggcgg cagctggaat tcggttggtt atgatgtgcg
    15781 taatgaaagc tttgtctcat tccaaaaacc tacttccaca gttggatttc gtccggttat
    15841 tatttgggaa gaataaactc ccaaaagcaa cggaacattc caacctaacg tcttgcgaat
    15901 agtagaaagt gctttagctt tctgcttgta tctcattcag ccatgaacag aaaagaattt
    15961 ttatccatcc tcggcatttc tgctggtggc ttggtcgttg caacctgttt aggaagttgt
    16021 aaaaaaaccg aaacgaatcc agttgatttt acaatcgacc tgagcaactc aagctattca
    16081 gcactcaata cgaatggaaa tttcttggta actaaaggtg tcataatcgc aaaaaccaat
    16141 acaggtagtt ttattgccgt tgctgccgct tgtacacacg aaggaacaaa tgttacttat
    16201 caagcagcca ataatcgatt ccattgcaat aatcatggtg ctaattttag tagcaacggc
    16261 gcagttcaaa atggtccggc aaatactgcc ttacaacaat ataacaccga attaaccgga
    16321 acaacgctta ggatttattc ctaaaaatat cattccatgt aagttgattc tcatttgagg
    16381 cttctcaacc taatgccgaa taaatagatt tgcaaaaatc ccattcagct caagaatgaa
    16441 tcaacgtata agttttatca cgcttggtgt gaagaatctt cagataatga ggacctttta
    16501 tacccaagtg tttggctgga ctttactcaa agacagcgat ggaattgtat tctttaaatt
    16561 aaatggcttt atactaggtc tctttccatc tcacgaatta gccgaagatg caaatgtgtc
    16621 ggagattggt agtggtttca agtcattcag tttatctatc aacttgaatt cagaaaagga
    16681 agttgatctt ttgtttcaag aactaatcag caaaggagcc cgaagtttaa aatcgcccga
    16741 acgtgtattt tggggtggat atcgtggtta tattgcagat cctgaagata atctttggga
    16801 attagcttat aacccctttt taaaaatgga tgaaaaagga aatgtagttc atcatccata
    16861 aattgctttc atttattata aacgctagga ataattcaac atgaaacaga aagaaaagat
    16921 atgggcatta gctttaatcc ttagtcttgg cttatttttt catgctaaaa acctcaacga
    16981 gtttccttta tggacacatg cttgggcgca gtcggatcgc tttgctattg cattaggatt
    17041 tttagataat ggtt
//
LOCUS       DAOOQW010000033        17009 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_4101_length_17009_cov_5.684086, whole genome shotgun sequence.
ACCESSION   DAOOQW010000033 DAOOQW010000000
VERSION     DAOOQW010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 17009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17009
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4101_length_17009_cov_5.684086"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            287..1471
                     /locus_tag="PK281_03600"
     CDS             287..1471
                     /locus_tag="PK281_03600"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOY48458.1"
                     /translation="MSKFLTRIFVVEDNEFFSQLIKQKLEMNDKNEVTLFHNGEDFQR
                     NLYLNPDIVVLDYNLPNTTGIDLLKEIKNFNEEIKTILLSGQEKVDVVIEAFENGAEE
                     YIKKDDNALQLLDLKVQKHSSSVNLRKEVDTLREQIIDRNRYDRIIGESNALLKVLRL
                     IQKVEKTNMLALITGESGTGKELVASAIHYNSARKNRPFVAVNVAAIPEDLMESELFG
                     HERGAFTGADSKRIGKFEEANEGTIFLDEIGEMDIHMQTKLLRVLQESKITRLGSNKE
                     IPLNVRVIAATNKNLAQRVKDGKMREDLYYRLQGFLIHLPPLRERDNDILLLAKSMLK
                     IFCDANRIPVKSFSSDALKAMMKHPWSGNVRELKAFIERAVLISDHDIIEEEDLIFSQ
                     DI"
     gene            complement(1527..1745)
                     /locus_tag="PK281_03605"
     CDS             complement(1527..1745)
                     /locus_tag="PK281_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4287 domain-containing protein"
                     /protein_id="HOY48459.1"
                     /translation="MSFQAYIDNIESKTGKSLADFKRMAEEKGFLINGKLAPSIKATA
                     IVDWLKADFALGHGHCMALFAAFKGKTS"
     gene            1883..2326
                     /locus_tag="PK281_03610"
     CDS             1883..2326
                     /locus_tag="PK281_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48460.1"
                     /translation="MNSQVGKKQAGIWLDHVEALLIDPYASLTNIERIQSDYESQVRE
                     PGESGDGRKLGANRSSNNEYSKHSIEQHDLHAFFKKVADSADHYNELYIFGPGTAPKE
                     FAHFLEQDKRFKNVLITTARADYLNESKLQEEVKDFFQQSLSTSK"
     gene            complement(2377..3759)
                     /locus_tag="PK281_03615"
     CDS             complement(2377..3759)
                     /locus_tag="PK281_03615"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2341 domain-containing protein"
                     /protein_id="HOY48461.1"
                     /translation="MKRKLQLRTKTKVKLRTSVKLAYAGAALSLMLIGGWVIYTNFGT
                     STDSLAGNEKYLPDFNWRRKLTFGNDLIKGKETFLNFPLLVQVSDPDFKSISNGGKVV
                     HPKGYDIRFTKADGLTIYPSQIDSYNPKTGELSAWVLLDTLSEKSLKDLYVYYSNATI
                     QAELPNILWSDAYRGIWHMNDLHASNNRKLRAITQGTAKATGKVGSAYMFQASNKDAA
                     FYPYSEEIDLKSDFSLSAWVYLNELSREQVILSNQGDRSGGYRLFISADNKLSVSYSN
                     ASGKLISTVDVSGGETLEKERWYYVAATYSVKENSVKTYVDGLLDRSLATIDSPGATS
                     SSLQLGRDLFNQSTYFNGMLDEVRIAAQANSQAWFATELYNQTLGKQLFTLSLADEIS
                     LDLASVNKNKESMNKQNAETQAQQAKANELRAQKVPATGEAPATLTTSAEAIQARMSS
                     IRRVAKDNEK"
     gene            complement(3853..4344)
                     /locus_tag="PK281_03620"
     CDS             complement(3853..4344)
                     /locus_tag="PK281_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48462.1"
                     /translation="MKPTLSNTKNTLVRKSLAALFIALGGMSAEAQTKMGKGLTGHAQ
                     LKPDDQNAISSFSPVYNNGKTFIRWLVTNDEKDGIFIIERSVDGTEFEALGFKDRVGS
                     PLCVNLFYSFIDEAPAAGQNYYRIMSVGTDQTYTYSDVVRVRTDVQNNTPPGTSASNE
                     NGQ"
     gene            complement(4684..>5895)
                     /locus_tag="PK281_03625"
     CDS             complement(4684..>5895)
                     /locus_tag="PK281_03625"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY48463.1"
                     /translation="WHLKGALNSKIPHSNQYSQQVNPDGSKEVYVASEKNSSQFPCLN
                     TPEDVDITGDITLSAWVYISDKKEQTIISNQNGFNGGYRLGINKDHRVEFIVRNQNAE
                     PASIAGEEGTELLKEHWYHVAAVYSDEGDSMATYINGKLDRSLKTEISMAGSTEPLQI
                     GREPSRKIFYFGGKLDEVHVSNIVRSSAWINTEFASQAKPQEFVKAGPTEAIVQQISM
                     SLLTFEGEAQGSTVELKWLTASEVDNELFTIERSSDGISFASIGTKPGAGNSNEVLSY
                     TYRDSKPLIGTNYYRVKLTGAAGEEFSMITPVNVESAGEEDIRISAAQPNPFVKDFKV
                     EYVVPKNGTARVKLMSVSGEVVMEEEVTCEKAATRQFIYKDEQGLKPGVYFFSVAQED
                     ETKMVKLIKRI"
     assembly_gap    5896..5995
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5996..6225)
                     /locus_tag="PK281_03630"
     CDS             complement(<5996..6225)
                     /locus_tag="PK281_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48464.1"
                     /translation="MKKRSTRLVPKVKTKVKLQHRLRYVAVAGAFALGVFSVALLYNT
                     IGTSNQSIANNKFNGVETLAEYKFRKQLSIDDT"
     gene            complement(6252..6737)
                     /locus_tag="PK281_03635"
     CDS             complement(6252..6737)
                     /locus_tag="PK281_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48465.1"
                     /translation="MKRSYLFTSLLAGLFMILSGMEVSAQSVSNSGSALLNKNSLKQD
                     SANTFISFSPVYLDGKTYVRWLVKNDKKDGVFVVERSENGTDFEALGFRDRVGTQLLV
                     NLFYSFVDEEPIPGDSHYRIMQVGADNTYRYSSVVKVHTPIEPRQVGSSANADAPVPT
                     K"
     gene            complement(6968..>8017)
                     /locus_tag="PK281_03640"
     CDS             complement(6968..>8017)
                     /locus_tag="PK281_03640"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY48466.1"
                     /translation="TVSAWIKTRGSAYDQTVITNLSRTGGFSLWIDDENRPVFEIANA
                     DGKIATLNKSNGGQALEKDTWYQLTGVYSAKSDSLAIYVNGKIDRSIKAGLAYAAGER
                     INIGVAPDQESSFFNGIIDEVRIAAKAYSNNEIATCWISESDPESFFSIDGKEVFSAS
                     PRIVQFGALEVKENSGHVVLNWQTTQERNLDFFNLERSSDGVHFQKVATKLGAGNSDE
                     ARNYFLIDPSPIYGETTYRIRSTSFKGESDVSRLLNVHVDAPATALGIRSVEPNPFRE
                     KFAVTYRSQSETSVQVKLTSISGQVVHTASVDPKAGADNLFEFKEATALRPGIYFLSL
                     SQADEQKTIKIIKQL"
     assembly_gap    8018..8117
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8118..8530)
                     /locus_tag="PK281_03645"
     CDS             complement(<8118..8530)
                     /locus_tag="PK281_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48467.1"
                     /translation="MKQRLRLGNRLNTRLRARLRVTAMVFTFGALVASIFFLYEFIGN
                     VTTGRAQKLEEQLHPKAGVLDGFAGRKKISIHPEKLPLGETLKQFTVLVSLRLDELKL
                     NSEGGMVSGRDGEDIVFTAIDGTTILPFQIERFEPA"
     gene            complement(8551..9054)
                     /locus_tag="PK281_03650"
     CDS             complement(8551..9054)
                     /locus_tag="PK281_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48468.1"
                     /translation="MENLNVFLSFNRLIKIGLVGAGLLIFGNTSQAQTPQTFKVAPPA
                     EAKPLLKDTVDNFLSFSPVYVEGKTYLRWLVENDKKDGVFIVERSADGEKFDALGFKD
                     RVGTDKNVNLFYSWVDDAPPAGAAHYRIMQVGVDHTFNYSDVVRVKTSAAASTGGSAS
                     STPEQGK"
     gene            complement(9637..11520)
                     /locus_tag="PK281_03655"
     CDS             complement(9637..11520)
                     /locus_tag="PK281_03655"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013871021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HOY48469.1"
                     /translation="MAENTDSTYNDDSIKSLDWKEHIRLRPGMYIGKLGDGSSLDDGI
                     YVLLKESIDNCIDEYVMGFGKKIEITIKDRMVRVRDYGRGIPLGKVVDVVSKINTGAK
                     YDSVAFKKSVGLNGVGTKAVNALSSYFKVQSVRDGKMKMAEFEKGNLTNETAVTDTND
                     NKGTLVQFFPDEAIFGNYKFISEYVEKMLWNYAYLNTGLSIHFNGQVFKSDRGLYDLL
                     EQNLSGEPLYPIIHLRGEDIEIAMTHGNQYSEEYYSFVNGQHTTQGGTHQAALREAVV
                     KVIREFYKKDFDANDVRQSIIVAISVKVVEPVFESQTKTKLGSQNIAPDGPAIRTFVI
                     DFLKKELDNYLHKHADVAEALLHKILQSEKERKELSGIRKLARERAKKVSLHNKKLRD
                     CRVHYNSNHAVRLDTTLFITEGDSASGSITKSRDVNTQAVFSLKGKPLNCYGLTKKIV
                     YENEEFNLLQSALNIEDGMDDLRYNNIVIATDADVDGMHIRLLMITFFLQFFPDLVKN
                     GHLYILQTPLFRVRNKKDTYYCYSEDERREAIVKVGKNPEITRFKGLGEISPDEFKHF
                     IGKDIRLEPVIFKREENLPQLLEFYMGKNTPDRQLFIIDNLKVEKDLVEEEKAAPVAQ
                     IED"
     gene            12356..12991
                     /locus_tag="PK281_03660"
     CDS             12356..12991
                     /locus_tag="PK281_03660"
                     /inference="COORDINATES: protein motif:HMM:NF025946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOY48470.1"
                     /translation="MIKVILVLGEFVVLCGMLLNSTLIRNAFTWERYMALMEQLVADK
                     QTTGEQTEEHIHFTELNIHRLKRVYKTLKFDSEVANALAETPRQLWVVISEAWCGDAS
                     QLLPVMARMADLSATIELRIVLRDENPELMDQFLTNGGKAIPKLICMNPDTFEVMWNW
                     GPRPLPAVELAKSIKSQGGTKEEIGLAMQEWYNKDKGQTTIQELLGYLTKP"
     gene            13051..13716
                     /locus_tag="PK281_03665"
     CDS             13051..13716
                     /locus_tag="PK281_03665"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOY48471.1"
                     /translation="MSARQIAVGDIHGCVRTFRELVRTKLNIQSGDQLILLGDLIDRG
                     PDTKGVIDEIFALKSEGVRVHVLQGNHEELMIAAYYGVDQMEYWRRCGGQDTLNSFGV
                     SSVLEIPIKYREFIQYSEMYFETPECIFVHAGLNFSKRDLFADLNAMRWIRKMEIDPE
                     RIGYRKIVHGHTPRPYTEIIKQNKYDINLDAGCAYTELPGMGYLVAYEVGTGEFTYVQ
                     NCE"
     gene            complement(13718..14818)
                     /gene="ychF"
                     /locus_tag="PK281_03670"
     CDS             complement(13718..14818)
                     /gene="ychF"
                     /locus_tag="PK281_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HOY48472.1"
                     /translation="MALKCGIVGLPNVGKSTLFNCLSNAKAQAANFPFCTIEPNVGVI
                     TVPDERMTELSKFVKPERIVPTTVEIVDIAGLVKGASKGEGLGNQFLGNIRECDAILH
                     VLRCFDDDNVVHVDGRVNPVSDKEVIDLELQLKDLDTVEKRLQKVEKLAKTGDKDAKR
                     AHDILSQYREVLLQGKSARSVKLEKVDDIEFANDLYLLTNKPVMYICNVDEASAKSGN
                     AHVEAVRASIADENAEILIITAAMEAEIAQLETYEDKVAFLSEIGLEEPGVNKLIKKA
                     YHLLNLQTYFTAGVKEVRAWTITTGMKAPQAAGVIHSDFEKGFIRAEVIKYLDFLKFG
                     SEASCRENGKLAVEGKEYVVQDGDIMHFRFNV"
     gene            complement(14895..>17009)
                     /locus_tag="PK281_03675"
     CDS             complement(14895..>17009)
                     /locus_tag="PK281_03675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOY48473.1"
                     /translation="IYKRSELIGRTMMEKFPGIEETEFFNILLKSTKEQIQQKIDIKF
                     TFPDHSTGWYEMCVQPIPEGLLILSLDKTAHILMEQKLNSNVEFNHKVLASLSAQILV
                     IEEDGMVLTVNKPLDDFLIEFGFLNKNDTSLGRNYFKLLHAVLSSNPLLADSFENGVH
                     MVFKNQLSVFEIECPVHLNEATRWFSLCVVPLEIDSDKVVISHQDITSRKVISENLKV
                     SEIKYRRLFESAQDGILILDAETGKIVDVNPFLQHLLGYTFNHFIGRELWEIGLFKDA
                     TASKESFLELQNKLYIRYEDLPLQTKDGNKIYVEFVSNVYFVNDEKVIQCNIRDISNR
                     IKIEESLSKFQSNLKAVIENTDADIYSLDKNLNYTTFNNNLQITIKNEFDLEVNYGDS
                     VFEFLSKNEPDLIQFWVNTYSKALSGEVVKFEKHISKGNKDQYTSYSIFPIWDNNRTV
                     GVSCFVLDITDKKEQEINKNKITNDLMQRNKDLEQFTFIVSHNLRAPVANIIGLTSLL
                     KDEYSLNEDKLKIINGLELSITRLDEVIGDLNNILQLRVQIDEPKKNIDLKNLIHDIQ
                     VSISNTVVNIEMDIQTNFKLCTEIKSIKSYLYSIFYNLISNSIKYRKQEEKLIIQISS
                     SISKNHIELIYHDNGIGIDLLKNGNQVFGLYKRFHLNQAEGKGVGLYMVKTQVEALGG
                     SISIESEINKGARFKILFPLPV"
BASE COUNT         4906 a         3265 c         3411 g         5227 t
ORIGIN      
        1 taataactct tctcctctcc aataacactt actttagcgt agctgagagg tttattatta
       61 gatccattaa gtaccttgat ttcttgggca attgcattga tgtgtatgaa acaaaaaagt
      121 aataataggt tgctaaagcg catgcttttt tttcttcaaa aatagactta ctaatttctt
      181 agaagaatga aaaacggatt taattttcac gtaagtttct gaataaatcc gtcggaacta
      241 cctagatgtt gtcccgtaca aagcgctaaa ctttaatctg ctacctatga gtaaattcct
      301 tacgcgaatt tttgtagtcg aagacaatga attcttcagc cagctaatca agcaaaagct
      361 ggagatgaac gacaaaaacg aagtaacgct tttccacaat ggagaagact ttcaacgcaa
      421 cctttacctc aaccccgata ttgtagtact tgattataat cttcctaata ctacgggaat
      481 tgatttattg aaagagatca agaatttcaa tgaggaaatc aaaaccattt tgctttctgg
      541 acaagagaaa gtagatgtag taattgaagc atttgaaaat ggtgcagaag aatacatcaa
      601 gaaagatgat aatgcactac aactgctcga tttaaaagta cagaaacaca gcagcagtgt
      661 taacttacgt aaagaagttg atacactacg cgaacaaatt atagatcgta atcgctacga
      721 ccgcattatt ggagaaagta acgctttatt gaaagtgctt cgcttgattc aaaaggtcga
      781 aaagaccaat atgttggcac ttattacggg tgaaagcgga acgggtaaag aattagtagc
      841 ttctgcaatt cactataatt cggcaaggaa aaatcgtccg tttgtggcgg ttaacgtggc
      901 agctattcca gaagatttga tggaaagtga attgtttggc catgagcgcg gcgcctttac
      961 tggtgcagat agtaagcgaa ttggaaagtt tgaagaagcc aacgaaggca ccattttctt
     1021 ggatgaaatt ggggaaatgg atattcacat gcagaccaaa ttactgcgtg tattacaaga
     1081 aagtaaaatc actcgtttag gttcgaataa agaaattcca ttgaatgtgc gcgtgattgc
     1141 agctaccaat aaaaacttag cgcaacgtgt gaaagacggc aaaatgcgtg aggatttgta
     1201 ctaccgttta caaggatttt taattcacct tccaccattg cgtgaacgtg ataacgatat
     1261 tcttctcctt gcgaaaagta tgttgaagat tttctgcgat gcgaatcgta ttcctgtaaa
     1321 aagcttttca tctgatgcat taaaagccat gatgaaacat ccttggagcg gaaatgtgcg
     1381 tgaattaaaa gcttttatcg aacgagccgt tttaatctct gatcacgata ttatcgaaga
     1441 agaagatttg atattctctc aggatattta aaattaaaca ctcaatctaa atccccgttt
     1501 gaaacattca ggcggggatt tttttattag gatgtcttgc ctttgaatgc tgcaaataaa
     1561 gccatgcaat gtccgtgacc taatgcaaaa tcagctttta gccagtcaac aattgctgtc
     1621 gctttaattg atggagccaa ttttccatta atcaaaaatc ctttttcttc tgccattcgt
     1681 ttaaaatcgg ctaatgattt tcctgttttg gattctatgt tatcgatgta ggcttgaaat
     1741 gacatactgt ggaattgata ctctacaaat ttgtagaaaa ttcagcttga gaagatacgg
     1801 gatgaaaatc aagtaaaata ttgattttca gattttttac ttcgccccgt ttattaaact
     1861 accttaacag aaaactaaaa ttatgaattc tcaggtagga aaaaaacaag caggtatttg
     1921 gctcgatcat gttgaagcat tattgattga tccttatgca tcattaacca acattgagcg
     1981 tattcaatcg gattatgaaa gtcaagtaag agagcctgga gaaagtggtg atggaaggaa
     2041 actgggtgct aatcgatctt ctaataatga atattcgaaa cattcgattg agcaacacga
     2101 cttacatgca ttttttaaaa aagtagctga ttctgctgat cattataatg agttgtacat
     2161 ttttggacca ggaacagcac caaaagagtt tgctcatttt ttagagcaag acaaacgttt
     2221 taaaaatgta ttgataacaa cagctcgtgc tgattattta aatgaaagta aattgcagga
     2281 agaggtgaag gatttttttc agcaatcgct tagtacaagc aaataaaaaa gggaacaaac
     2341 atttcgtcgg ttcccttatc tatactgtat tgtaaattat ttctcgttgt cttttgctac
     2401 tcgacggatg gaactcattc gagcttgaat agcctcggca cttgtggtta aagtagcagg
     2461 agcttcgcca gttgctggca ctttttgagc acgtagttca tttgctttcg cttgttgtgc
     2521 ctgagtttct gcattttgct tattcatgct ttccttattc ttgttgacag aagctaaatc
     2581 gagtgaaatt tcatcggcaa gacttagtgt aaacaactgt ttcccgagtg tttggttata
     2641 taattcggta gcaaaccaag cttgagaatt ggcttgagct gcaattcgaa cttcatccaa
     2701 cattccattg aaataggtag attgattgaa taaatctcgc cctaattgca aagaagaaga
     2761 agttgcacct ggtgaatcga ttgtggctaa acttcgatcg agcaaaccat ctacataggt
     2821 tttaacacta ttttctttta ccgaataagt agctgcaaca taataccagc gctctttttc
     2881 gagcgtttct cctccactaa catcaactgt agaaattagc tttccagaag cattgctgta
     2941 agatacactc agtttgttgt cggcactgat aaacaaacga tatccacctg agcgatcacc
     3001 ttgatttgaa aggataactt gctcgcgtga tagttcattg aggtaaaccc aagcagaaag
     3061 actgaaatcg gattttagat ctatttcttc ggaataagga taaaatgctg cgtctttgtt
     3121 ggaagcttga aacatataag cagatccaac tttaccggta gctttagcag taccttgtgt
     3181 gattgctctc agttttcgat tgtttgatgc atgtaaatcg ttcatgtgcc aaatacctcg
     3241 ataagcatca ctccacaata tattgggtaa ttcagcttga atagtagcat tgctgtaata
     3301 cacatacaaa tccttcaaac ttttttctga taaagtatcg agtaaaaccc aagcagaaag
     3361 ttctcctgtt tttgggttat aactatcgat ttgagaagga taaatggtta agccatcggc
     3421 ctttgtaaat cgaatatcat atcctttagg atgaactact ttaccaccat tactgatgga
     3481 tttaaaatcg ggatcagaaa cctgaacgag taaaggaaaa ttcaagaatg tttctttgcc
     3541 cttgataagg tcgtttccaa atgtgagttt gcgtctccaa ttgaagtcag gaagatattt
     3601 ctcattccct gccagactat cggttgatgt tccgaagttg gtgtatataa cccatccgcc
     3661 aattaacata agtgacaaag ccgcaccagc gtatgctagt ttgacagatg tccttagttt
     3721 gactttggtt ttggttcgga gttgtagttt tcttttcatg agctaggttg tgcctcgcag
     3781 ctatagaata aacaatcccc aggcatcgta aataaatgat acggtgcctg aggatggaag
     3841 tttcaaggat tgctattggc cattttcatt agatgcgcta gtgcctggcg gagtattgtt
     3901 ttgtacgtcg gttcttaccc gaacgacatc gctgtatgtg tacgtttgat ctgtaccaac
     3961 tgacatgatg cggtagtaat tttgtcctgc tgctggagct tcgtcgatga acgagtagaa
     4021 cagattcaca caaagaggtg aacctacacg atctttaaag ccaagggctt cgaattcggt
     4081 accatcaacc gatcgttcaa taatgaaaat tccatctttc tcatcgtttg taaccagcca
     4141 acggataaag gtttttccat tgttgtaaac gggcgagaac gatgaaatcg cattttgatc
     4201 atcgggttta agttgagcgt gccccgtgag acctttaccc atcttcgtct gggcttcggc
     4261 cgacatgcct cctaaggcaa tgaataacgc tgcgagcgat tttcttacca aggtattttt
     4321 ggtgtttgaa agagtgggtt tcatatatcg tcaggttagg gctgttgatt gttctatttg
     4381 atacgtaaag ctattgtcag cgttcctttt gatttatccg attcagatag tagcactaca
     4441 ggtgtaagaa aatgatatgc atgaacacaa taagatattt cccaattcca aaacacttat
     4501 ttaacaagcc ttgcagaagt tttggctcga agagaaatga aaaacaccta cgtaagaatt
     4561 tgtactttgg agtgtgtaat tcaagatttt tgtccaattc cttcaaaaag taatttgaac
     4621 ccataaaaaa accccgtgct atatcagcga cggggtttcc aaagttgagg ttagggaata
     4681 cttttaaata cgtttgatga gtttcaccat tttagtttca tcttcttgag caacactgaa
     4741 gaaatacact ccaggcttta gtccttgctc atctttgtag atgaattggc gcgttgcagc
     4801 tttttcacag gttacttctt cttccatcac tacttcgccg cttacactca taagtttcac
     4861 acgagcagtt ccgtttttag ggactacgta ttctacttta aagtctttga cgaaaggatt
     4921 tggttgcgca gctgagattc ggatatcttc ttcacccgcc gattcaacat tcacaggagt
     4981 aatcatggag aattcttctc ctgcagcacc agtaagtttt acgcgatagt agtttgttcc
     5041 gataagtggt ttagaatcac gataggtata gctaagcact tcgttactgt ttccagcacc
     5101 cggtttagtt ccgattgaag cgaagctgat tccgtcggaa gaacgttcga tggtgaaaag
     5161 ttcgttatcg acttcagaag cagttagcca tttcaattct actgtacttc cttgagcttc
     5221 cccttcgaat gtgagcaaac tcatagagat ttgttgtacg atagcttcgg ttggtccagc
     5281 ttttacaaat tcttgaggtt tagcttgact tgcgaattcg gtgttaatcc atgctgagct
     5341 acgaacgatg ttactaacgt gaacttcgtc gagtttacct ccgaagtaga agattttgcg
     5401 agatggttca cgaccaattt gaagtggttc ggtgctacca gccatgctga tttcggtttt
     5461 taaagaacga tcgagttttc cgttgatgta agttgccatt gaatctccct catcggaata
     5521 aacagcagca acatgatacc aatgctcttt tagcaattca gttccttctt caccagcaat
     5581 agaggccggc tcagcatttt ggttacgaac gatgaattcg acacggtgat ctttattgat
     5641 tccgagacgg tatccaccat taaatccatt ttgattggag atgatggttt gttctttttt
     5701 gtcggagatg taaacccaag cagataaagt aatatcgcca gtgatatcca catcttcggg
     5761 agtatttaag caagggaact gagaagaatt tttctctgaa gccacatata cttctttact
     5821 accatcagga ttaacctgtt gagaatattg atttgaatgt gggattttag agtttaaagc
     5881 tccttttaag tgccannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngtatc
     6001 gtcgatgctg agttgttttc tgaatttata ttcagccagt gtttccacac cgttgaattt
     6061 gttgttagct atcgactgat tggaagtacc aattgtgtta tacaatagcg caactgaaaa
     6121 tactccgagt gcgaaagcac ctgccaccgc cacataacgc aaacgatgct gaagtttaac
     6181 cttggtttta accttaggta ctaatcgagt agaacgtttt ttcataagaa atgagcttgt
     6241 gtgaggtgta attatttagt tggtaccggt gcgtcggcat ttgctgatga tccaacctga
     6301 cgaggttcga tcggagtatg aacttttaca accgaactgt agcgatatgt gttatcagca
     6361 cctacctgca tgattcggta atgactatca cctggaatag gttcttcgtc aacaaacgaa
     6421 tagaatagat ttacaagaag ttgtgttcca acgcggtcgc ggaaaccaag ggcttcgaag
     6481 tcggtgccgt tttcggaacg ctccactacg aatacaccgt cttttttgtc gtttttaacc
     6541 agccagcgta cgtatgtttt accgtcgaga tatactggtg aaaatgaaat aaacgtgttg
     6601 gctgaatctt gcttaagtga gttcttatta agcaatgcgg aaccgctgtt ggaaaccgat
     6661 tgtgccgaaa cttccattcc tgaaaggatc ataaacaagc cggctaataa actggtaaaa
     6721 aggtaactgc gtttcatagt gtttcgttgt tgaactttgt tacgtcaaag atatctgggg
     6781 tgtttggggc agaattcaga aggtgattct cagcattaca gatgtaagtt ttgaaatgca
     6841 tgtgtttcac ttttttatcg ctcttcctta tttaacagct atctcagcga gtttagaatt
     6901 tgtgttaggt tagtttgatt tgtaagattg tatgtcgaag aaccgttttg atccttttta
     6961 tgacttttca gagttgtttg atgattttga tggttttctg ttcatcagct tgcgataagc
     7021 tcaagaagta aattccaggt cttagtgcag ttgcttcttt gaattcgaac agattatcag
     7081 cacccgcttt aggatcaaca gaagctgtat gaaccacttg accgcttata ctggttagtt
     7141 ttacctgtac tgaagtttcg ctttgagaac gataggttac agcgaatttt tcacggaacg
     7201 gattgggttc cacgctacga atacctaatg ctgtagcagg tgcatccaca tgaacattca
     7261 acaagcgact cacatcactt tcacctttga agctagttga acgaattcgg tatgttgttt
     7321 caccatatat aggtgaagga tcaattaaga aatagttgcg cgcttcgtct gaatttccag
     7381 ctccaagttt ggtagctact ttttgaaaat gaacaccatc ggaagagcgt tctaaattga
     7441 agaaatcgag gttgcgttct tgtgttgttt gccagttgag taccacgtgt ccggagtttt
     7501 ctttcacttc gagtgcgcca aactgaacga tgcgaggaga agcactaaac acttcttttc
     7561 catcgatact aaaaaagctt tcgggatcgg attcggaaat ccaacatgta gcgatttcgt
     7621 tgttggaata tgcttttgca gcaatccgaa cttcatcaat aattccatta aagaagctgc
     7681 tttcctgatc aggagcaaca ccaatattta ttctttctcc agctgcataa gctaacccag
     7741 cttttatact gcggtcaatt tttccattca cataaatagc tagtgaatca gattttgctg
     7801 aatacacacc tgtgagttgg taccaagtgt ctttttcaag tgcttgtccc ccattcgatt
     7861 tattaagtgt ggcaatcttt ccatcggcat tagcaatttc gaaaacggga cgattttcat
     7921 catcaatcca taaactgaaa ccacctgtgc gcgataaatt cgttataacc gtttggtcgt
     7981 aagcacttcc gcgggtttta atccaagccg atacagtnnn nnnnnnnnnn nnnnnnnnnn
     8041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8101 nnnnnnnnnn nnnnnnngct ggttcgaaac gctcgatttg aaagggtaaa atggtagttc
     8161 cgtcgatggc agtgaacacg atatcttctc catcgcgacc cgaaaccatt cctccttcgg
     8221 aattaagttt taactcatct aaacgaaggc taactaatac agtaaattgt tttaaagttt
     8281 ctcccagagg aagtttctcg ggatgaatgc taattttttt ccgaccagcg aatccatcca
     8341 atacgcctgc tttagggtga agctgttctt ccaatttttg agcgcggcca gttgttacat
     8401 taccgataaa ctcatacaga aagaagatgc ttgctaccag agcgccaaag gtgaatacca
     8461 ttgcagtaac tcgcaaacga gcgcgcaatc gggtattcag acgattacct aaacgtaaac
     8521 gttgtttcat gctggttgga tttaatgggg ttatttacct tgttcaggcg tagagcttgc
     8581 gctaccaccc gttgatgctg ccgcagaagt tttcacacga actacatcgg agtagttaaa
     8641 tgtgtgatct acacccacct gcataatgcg gtaatgtgca gcacctgcag gaggagcatc
     8701 gtccacccag gaataaaaca gattcacgtt tttatcggtt cccactcggt ctttaaatcc
     8761 aagtgcatcg aacttttcgc catcggccga acgctctaca atgaaaacgc cgtccttctt
     8821 atcgttctca accaaccaac gtaaataggt tttgccttct acgtataccg gtgaaaagga
     8881 taggaaattg tccaccgtgt ctttcaaaag cggttttgct tctgcaggtg gtgcaacttt
     8941 aaatgtctga ggcgtttgag cctgcgacgt gttgccaaaa attaaaagcc cggctccgac
     9001 gagtccgatt ttaataaggc gattaaatga aagaaaaacg ttgaggtttt ccataggata
     9061 aggttttgga ttgctcaacg acaaaaggca aaaagcggtt gcctgaatag ctgcctcaga
     9121 agcagcactc tttacagatg taagaagata ttattttgcg aatacttaat tttcgcaatg
     9181 cctttaaata agggtattct aagaagaata ttattttcaa gcctgcaatt ccagaagtaa
     9241 gaaaaaagcg aattgttaga aatggatagc atttctaaca attgtgaacg aaaaaccaag
     9301 aaataagcta caaatcgaag ggataatttc aactacttaa ggcttcactg gacaagtttt
     9361 ctagtttcca aaacacaact ctctcatccc taaatttcaa tccggggcag agtgttcaac
     9421 aacaacgtat ctctgattcg acaaactagc atcctcggat ttcaattcaa ggcggagtgt
     9481 tcaactaaaa cgtacctcag cttctatatt ctagcacccc cggatttcaa tccggggaac
     9541 taactaaaga gtttcaggtt aactgctaca aaaaaccccg gatttcaatc cggggttgct
     9601 gtaggttaaa tgtaagttct tgtaaatgag gctattttaa tcttcgattt gagctacggg
     9661 agctgctttt tcttcttcta cgagatcctt ttcaaccttg agattatcaa tgataaaaag
     9721 ctgacgatcg ggtgtgtttt tacccatgta gaattcgagc aattggggca ggttttcttc
     9781 gcgtttaaaa atcaccggtt cgaggcgaat gtctttacca atgaagtgtt taaactcatc
     9841 gggcgaaatc tctcctaatc ccttgaatcg ggtaatctct gggttcttgc ccactttcac
     9901 aatggcttcg cggcgctcgt cttcggaata gcagtaatac gtgtctttct tattacggac
     9961 gcggaaaagc ggtgtttgca aaatgtacaa gtgaccgttc ttcaccaaat cggggaagaa
    10021 ttgtaagaag aacgtaatca tcaacaaacg aatgtgcatt ccatcgacgt cggcatcggt
    10081 tgcgatgaca atgttgttgt agcgcaaatc gtccatgcca tcctcaatgt tgagggcact
    10141 ttgcaagagg ttgaattctt cgttttcgta aacgattttc ttggtcaaac cataacagtt
    10201 gagcggtttc cctttcaagc tgaatacggc ttgtgtattt acatcgcggc ttttggtgat
    10261 ggaaccagat gccgaatctc cctcggtaat aaagagtgtt gtatcgagac gaactgcgtg
    10321 gttgctgttg taatgtactc gacaatcgcg gagtttcttg ttgtgcagac ttactttctt
    10381 agcacgctct ctagccagct tacgaatgcc gctgagttct ttccgctctt tttcgctttg
    10441 aagaatttta tgcagcaatg cttccgcaac atcggcatgc ttgtgtaggt agttatcgag
    10501 ctcttttttg aggaaatcta taacgaacgt acgaattgcc ggaccgtcgg gagcgatgtt
    10561 ttgagaaccg agtttggtct tagtctgact ttcgaacacc ggttcaacta ctttcacact
    10621 aatagcaaca atgatggatt gacgcacatc attcgcatcg aaatctttct tgtagaattc
    10681 gcgaatcacc ttcaccactg cttcacgcaa tgccgcttgg tgtgtaccac cttgggttgt
    10741 gtgctgacca ttgacaaacg aataatattc ttcgctgtat tggttaccat gcgtcatggc
    10801 aatttcaatg tcctcgccac gaaggtgaat aattggataa agcggttctc cactgaggtt
    10861 ttgctctagt agatcgtata atccacggtc ggatttgaat acttgaccat tgaaatgtat
    10921 gctcaaacct gtattgaggt aagcataatt ccagagcatt ttctctacgt attcgctgat
    10981 aaacttgtag tttccgaata tggcttcatc cggaaaaaac tgaacaagtg ttcctttgtt
    11041 gtcgttggtg tcggtaacag cggtttcgtt ggtgagattt cctttctcaa actccgccat
    11101 tttcatcttc ccatcacgca ccgactgcac cttgaaatag ctcgaaagcg cattcactgc
    11161 cttggttccg acaccgttca aaccaaccga tttcttgaaa gctaccgaat cgtacttcgc
    11221 accagtattg attttcgaca ctacgtcgac cactttaccg agcggaatgc ctcgtccgta
    11281 gtcgcgcaca cgcaccatgc gatctttaat ggtgatttcg attttcttcc cgaaacccat
    11341 tacgtattca tcgatgcagt tatcgattga ttctttcagc aatacataaa tcccgtcatc
    11401 gagcgaagaa ccgtcgccca atttcccaat gtacatccca ggacggaggc ggatatgttc
    11461 cttccagtcg agggatttaa tggaatcgtc gttgtatgtg ctatctgtat tttcagccat
    11521 ggtgcaaaaa taaggattgc cgattgatgg gaaagcggga tagggagaga attattaacg
    11581 aaatgaagag ggattttcga tatacgatat acgatgtacg atttacgaat aggcaattac
    11641 gaataggcaa ttacgaatta cgaatgggga attacgaatg ttcaattacg aattacgaat
    11701 ggggaattac gaatgagaaa tgaagaggga tgtacgattt acgaaaattt tgggaggaga
    11761 ggcattcgtt ttttcaccct tacctcaatc tagctaattt gaatagtagc ctcggactga
    11821 agtccgaggg cgcgaatgtt attgataagc aatttgctgt atggttgata tatcgaatct
    11881 ggcttgtgtg attcgtagcc tcggactgaa gtccgagggt gccgattggt aatttgaagc
    11941 attagggcca attggattct agtagcctcc gaattcagtc cgaggatggg gatttatgag
    12001 atttgcagaa tatcccgcga cagggattgt agccattacc gcttttgaac cgaaggtgaa
    12061 aaagtagtag aggcggaaag cccggtgctt ttgtaacgaa gtggaaaaag caggcgccct
    12121 cattaaagta caacgattta cgtaatacgt attacaattt ttttcaaaga cttgcattcg
    12181 tgaattcacc acgacctcaa tttagcttaa atgaatagta gcctcggact taagtccgag
    12241 ggtgcggttg gttaaaatca gcaaaatttc aagcgaagta atcaaaatgc gaagcggtcg
    12301 taaatcgaac atcgtcttcg taattcgtaa ttgaacattc gtaattcttg cttccgtgat
    12361 aaaagtcatt cttgtattgg gcgagtttgt tgttctttgc ggcatgcttt tgaattcaac
    12421 acttatccga aacgctttta cttgggagcg ttatatggcg ctcatggagc aattggtggc
    12481 cgataaacaa accacgggag aacaaaccga agaacatatt cattttactg aactcaatat
    12541 tcaccgactc aagcgggttt ataaaacgtt gaagtttgat tcagaagttg caaatgctct
    12601 agcagaaacg ccacgacagt tatgggtggt gatttcggaa gcttggtgtg gtgatgcttc
    12661 tcagttgttg cctgtaatgg ctcgaatggc tgatttaagc gcaactatcg aactacgcat
    12721 tgtgcttcgt gatgaaaatc ccgaattgat ggatcagttt cttaccaatg gcggtaaggc
    12781 gattcccaaa ttgatttgta tgaatcccga tacatttgag gtgatgtgga attggggacc
    12841 acgaccatta ccagcggtgg aattagcaaa atcgattaaa tctcaaggtg gaacgaagga
    12901 agaaatcggt ttggcgatgc aggaatggta taataaagac aaaggtcaaa caaccattca
    12961 ggaacttttg ggttatttaa cgaagccata aaaagctatc gtgttaaacc aatgacgata
    13021 ttgcggcatt atttatttta aattatcgtt atgtcggccc gtcagattgc ggttggagat
    13081 attcatggtt gcgtgagaac ctttcgggaa ttagttcgaa cgaagttgaa cattcaatcg
    13141 ggtgatcagc ttattttatt gggcgattta attgatcgtg gtccggatac caaaggcgta
    13201 attgatgaga ttttcgcttt aaaaagtgaa ggcgttcgtg tgcatgtgtt gcagggaaat
    13261 cacgaagagt tgatgattgc agcctattat ggagttgatc aaatggaata ctggcggcgc
    13321 tgcggcggac aagatactct caacagtttt ggggtttcgt cggtgcttga gattcctatt
    13381 aaatatcgag aatttattca atatagcgag atgtacttcg aaactccgga atgcatattc
    13441 gtacatgccg gattgaattt ttcgaagcgt gatttatttg ccgatttaaa tgcgatgcgc
    13501 tggatacgca aaatggaaat cgatcccgaa cgtattggct atcggaaaat cgtgcacggt
    13561 catacacctc gaccgtatac cgaaatcatc aaacagaata agtacgacat taatttagat
    13621 gccggatgtg cgtataccga attacccgga atgggctatt tggtagctta cgaagttgga
    13681 acaggcgaat tcacttacgt ccaaaactgc gaataactta tacattgaaa cggaagtgca
    13741 tgatatcgcc atcttgcacc acgtattctt ttccttctac ggctagctta ccattttcgc
    13801 ggcaagaagc ttccgaacca aacttgagaa aatcgaggta tttaatcact tctgcacgga
    13861 taaatccttt ttcgaaatcg ctgtgaatta ctccagcagc ttgtggagct ttcatacctg
    13921 tggtgatggt ccatgcgcgg acttctttta cacctgcagt aaagtatgtt tggaggttga
    13981 gcaagtgata ggcttttttg atgagcttat tcacgcccgg ttcttccaga ccaatttcac
    14041 ttaggaaagc aactttgtcc tcgtatgttt cgagttgagc aatttcggct tccattgcag
    14101 cagtgatgat caagatttcg gcattctcat cagcaataga tgcacgaaca gcctccacat
    14161 gcgcatttcc cgatttggcg gatgcttcat cgacgttaca gatgtacatg accggtttgt
    14221 tggtcaacaa atacaaatcg ttagcaaact caatatcatc gactttctcc aatttcacac
    14281 tacgggcaga ttttccttgt aacaatactt cccggtattg agaaagaata tcatgagcac
    14341 gtttagcgtc tttatcaccg gtcttagcca acttctccac tttctgaagt cgcttttcta
    14401 cagtatctag atctttcaat tgaagttcta ggtcgataac ttctttatcg cttactggat
    14461 taacgcgacc atcgacgtga accacattat cgtcatcgaa acaacgcaaa acgtgcaaaa
    14521 ttgcatcgca ttcgcggata ttacctagga attggttacc taaaccttct cctttggaag
    14581 cacctttcac caaaccagca atgtcaacaa tctcaacggt ggtaggaaca atacgttcag
    14641 gtttaacgaa tttggacaac tcagtcatac gttcatcggg aacggtaatc acgcccacgt
    14701 tcggttcaat tgtgcagaac ggaaagttgg ctgcctgtgc ttttgcgttg ctcaaacagt
    14761 taaaaagagt ggatttccct acgtttggta atccgacaat accgcattta agtgccatag
    14821 tttttctgaa aatgggcgca aagatagcat tattggcggt ttgatatttt aaaaagaaac
    14881 gggtatagct aggattaaac tggaagggga aatagtattt tgaaccttgc tcccttattt
    14941 atctcacttt caatgctgat gcttccacct aaagcttcca cttgagtttt gaccatatac
    15001 aagccaacac cttttccttc agcctgattt agatgaaaac gcttatataa tccaaaaacc
    15061 tgatttccat ttttgagtaa gtctattcca attccattgt catgataaat caattcgata
    15121 tgatttttag aaatggagga agatatttga atgattagtt tctcctcttg ctttctatac
    15181 tttatactat tggaaattaa attgtaaaag atgctgtata gatagctttt aatagatttt
    15241 atttcggtac ataatttaaa attggtttgg atatccattt caatgttgac aaccgtatta
    15301 cttatactaa cttgaatatc atgaataaga ttctttaaat caatattttt ctttggttca
    15361 tcaatctgaa ctcttagttg tagaatatta ttcaaatcac caataacttc atccaaacga
    15421 gtaatagaaa gttctaaacc attaatgatt ttcaatttat cctcattcag agaatattca
    15481 tctttcaata aggatgtaag tccaataata tttgcaacgg gagcgcgaag attatgagaa
    15541 acaataaaag taaattgttc caaatcctta tttcgttgca ttaaatcatt tgtgatttta
    15601 tttttattaa tctcttgttc ctttttatcc gttatatcta aaacaaaaca agaaactcca
    15661 actgttctat tgttgtccca aattggaaaa atagaatagg aagtatattg atccttattt
    15721 cctttactaa tgtgcttttc aaacttaact acttcaccgc taagtgcttt agaatatgta
    15781 ttaacccaaa attgaattag atcaggttcg tttttactta gaaattcgaa aacactatcg
    15841 ccataattaa cttctaaatc aaattcattc ttaatggtaa tttgaaggtt attattaaat
    15901 gtggtataat tcaaattctt atccaatgaa taaatatcgg catcagtatt ttcgataaca
    15961 gccttcaaat tagattggaa tttacttagc gactcctcga tttttattcg attgcttata
    16021 tcgcgaatat tacattgaat tactttctca tcatttacga aatacacatt gctaacaaat
    16081 tcgacataaa ttttatttcc gtcttttgtt tgaagaggta aatcttcgta acggatgtat
    16141 agtttatttt gaagctctaa gaacgattct ttagatgcag ttgcgtcttt aaacaagcct
    16201 atttcccata gttctctccc tatgaaatga ttgaatgtat aaccaagtaa atgttgaaga
    16261 aaaggattta catccactat tttacctgtc tcagcatcta gaattaaaat tccatcctgt
    16321 gccgattcaa acaaacgtct gtatttgatt tcagaaactt ttaaattctc agaaataact
    16381 tttcgactag taatatcttg gtgagaaata acaactttat cggaatcaat ttccaatgga
    16441 accacacata aactaaacca gcgagtggct tcattaagat gaacaggaca ctcgatctca
    16501 aaaactgaaa gttgattttt gaaaaccata tgaactccat tttcaaaaga atctgccaag
    16561 agtggatttg aacttaaaac agcatgaagt aatttaaaat aattccgtcc tagcgatgtg
    16621 tcatttttat ttaaaaaacc aaattcaatt aaaaaatcat ctaacggttt atttaccgtt
    16681 aataccatac catcttcctc aataaccagt atttgagcac ttagtgaagc tagaacttta
    16741 tgattaaact caacatttga attcagtttc tgctccatta aaatatgagc tgttttatcc
    16801 aaggaaagaa tcagcaaccc ctccggaata ggttgaacac acatctcata ccagccagtt
    16861 gagtgatcag gaaacgtaaa cttaatatcg attttctgct gaatctgctc ttttgtactc
    16921 tttaataaaa tgttaaagaa ttcggtttct tcaatgcctg gaaatttttc catcattgta
    16981 cgtcctatta attctgatcg cttgtaaat
//
LOCUS       DAOOQW010000034        16410 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_4304_length_16410_cov_6.264506, whole genome shotgun sequence.
ACCESSION   DAOOQW010000034 DAOOQW010000000
VERSION     DAOOQW010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 16410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16410
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4304_length_16410_cov_6.264506"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..881
                     /locus_tag="PK281_03680"
     CDS             <1..881
                     /locus_tag="PK281_03680"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HOY48474.1"
                     /translation="MVEINAKGKVKLAVANALWNRLKLHPDYLAITKKYYNAEVYPLT
                     SETPINDWASKNTNGKIPKVLEPGDITPLVKLILTNAIYFKGDWVKTFDKENTQEAKF
                     YQSNKSISPCSLMYAKDYYRYGQFDGYQAIELPYSGDDLSMVVLLPKRESSLKQLFQT
                     LNTKELDTQLRALRSQQVELWLPKFKLETSYELKDVLMQMGMKAPFSRADFTKMTKDK
                     TPLEISRIIQKAFVEVNEKGTEAAAVTVVMMATESSISRHEIEIPQFRADRPFLFLIR
                     DKQTGAILFTGGLEKP"
     gene            926..3910
                     /locus_tag="PK281_03685"
     CDS             926..3910
                     /locus_tag="PK281_03685"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY48475.1"
                     /translation="MRNLLFTFAFFMLSIEAIVAQVNYNDDITISLTGAISAQNPGWP
                     FNGPYNLVPSGNLNGFIFSGSTPTCSASSYHTFGNFGSGWTTGQAQISQPSGWNYEAY
                     VDFGHTASWRCNVPLVGSTVNTSYAAIHGVVIGGALILKTTYGNTSHDDLREYMYVKL
                     ARPLMHGKTYHFRYTVAAAHPSGELTTANVWQDQLGVYLSPNPPNWAGAYTNVPVDTI
                     TPTSVTPAGQIISDTGITIEQDFIGNGEEWLMVGSFSPLDSIHYSGPHDVTFQSRYLF
                     DNFAIYDPNCENPIYLVLPQPPTTPYCHPTNPISCYSTTGFDDYRWYVDGVLQSDTSY
                     TFNYIPQHSTTISLAGTIGTCVDSISYFYPVQEVDFGLPADIFLFCGDPITLDPEIQT
                     NYPIQYSEWTNLANNQVFNGSGPYTISQYGDYEVHSMTISGCNFRDTLQVIPRLLPTT
                     NTQGQAIPVIQMRDVSCPGEDDGYAIYNFPGLDSLHYDWGIAGVSDTSTGIYNSPAQN
                     FNLRVYDNDQHCTVFYPSIINNFDSCAVINGTIYLDGDADCLLDTNELTVSSVMMYSP
                     EFDRMALTDSMGNYTLRIPPGPQHILQSSYNHPLMGTLCEDTVLVNPTAFNQIINGIN
                     FGDTITAPVYDAAIVFGGCAQLVPGHISYIPSVVQNTGNLPISFVFRQVISDSLWIDH
                     AEGYFYGTWDEIALQGINADTATWQLDLAPGQSAYFRTWVEIPPNPALVGSTLHSYSE
                     IIPLLQDDDSDNNHLDMNCLVIGSFDPNRKNVNPAGQGEQGLISAEVEDFQFLIEFQN
                     TGTAPADNIYVEDTLSSLFNLNSLQVMGASHAYQTEIRQGNLIRFIFPNINLADSLSN
                     EPESHGWIAFKVKRQANATNPGTEFQNTALIYFDFNEPVVTNTTLNTIAEDVVLSVPQ
                     KETAELEIYPNPAVNTIRILLPSKAKEIRISDISGRVLLRKTGDANLLHQLDIQSLSS
                     GMYLVDVTLNNGSIVQKKLIIR"
     gene            complement(3913..6687)
                     /gene="leuS"
                     /locus_tag="PK281_03690"
     CDS             complement(3913..6687)
                     /gene="leuS"
                     /locus_tag="PK281_03690"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HOY48476.1"
                     /translation="MEPNFRAIEKKWQQTWKDEKRYKTNIDLSKPKYYVLDMFPYPSG
                     AGLHVGHPLGYIASDIVSRYKRLKGFNVLHPMGFDAFGLPAEQYAIQTGRHPADTTAE
                     NIARYKEQLGNIGFCYDWDREVITSDPNYHKWTQWIFLQLFGCWFNLESQKAEDISKL
                     EALFSQGGSTAAKGACGEHAPFSAAEWNAMNEADQRGILMHYRLAYQDFSTVNWCEAL
                     GTVLANDEVKDGLSERGGHPVEKRRMRQWFLRITAYAERLLNDLETLEWTDSMKEMQR
                     NWIGKSEGASLKFQLENHPDQIEVFTTRPDTIFGVSFLTLAPEHELVSVITTDLQRDA
                     VNAYVEVAKNRSERERMADVKRISGEFTGAYVLHPFTGAKIPVWVGDYVLAGYGTGAV
                     MAVPAGDQRDWDFAKHFNLPIPVINEGVDISEAANPTKEAKMINSDFLNGLTGYEALK
                     KAIAAIEERGFGKGKVNYRLRDAGFSRQRYWGEPFPIVYRDGVAYPLSENELPLELPV
                     MADFKPTGQPESPLTKVPEWVNVPDGTRETDTMPGYAGSSWYFLRYMDPQNKARFVGR
                     EAEEYWQDVDLYIGGTEHAVGHLLYARFWQKFLFDRGEVSKNEPFRKLINQGMILGRS
                     NFVYQIIGTTKFISKDMLGEFKDEKILQTHVDVNIVQNDLLNLNAFKAWRDENAAAEF
                     ILNAEGNYVCGVEVEKMSKSKYNVVNPDDIINDYGADTLRLYEMFLGPLEQFKPWNTN
                     GIEGVYRFLKKLWNLYFDENGQVKLSDEAPTEAELRTLHKTIRKVEEDIEKFSFNTSV
                     SVFMICVNELSEQKCRKRAILQDLSVIIAPYAPHMAEELWQACGGSGSVVDAQFPQWN
                     ESFIKESSIAYPVAFNGKVRYQIQVAADMPQAEVQALALSHEDAAKWLPEGGPKKVIV
                     VPGRMVNVVI"
     gene            6868..7743
                     /locus_tag="PK281_03695"
     CDS             6868..7743
                     /locus_tag="PK281_03695"
                     /inference="COORDINATES: protein motif:HMM:NF037183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HOY48477.1"
                     /translation="MSSNENQFNRRRIRASYITSVVSIAMVLFMLGLLGLLLVDAKKL
                     SDTTRESFQVSVFFRDSVDDAQILAFKQELDTQPYVKSTTFITRAQAADEFQKDLGEE
                     FVDFLGYNPLPASIDVRFKVNFTNESDFQKIEKEWLANPLVAQVDYPRNLINQIIDNM
                     RKLSWFFLAFSAILTFIAVTLINNTIRLSIYSKRLLIRTMQLVGATRGFIRGPFLRAS
                     FMQGFAGGILAILLLSGMLYLGEQQIPELKPLRDFNLLGILAGGILLGGIGLSWICTF
                     FAVRKYLHLKTDSLY"
     gene            7753..7998
                     /locus_tag="PK281_03700"
     CDS             7753..7998
                     /locus_tag="PK281_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013620482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3098 domain-containing protein"
                     /protein_id="HOY48478.1"
                     /translation="MSTEKDNNQKGFAFSPMNYKIMLAGILVILVGFLLMVGGGSKDP
                     NVFNPEIFSTQRITVAPIVVLIGFAIEVFAILYKPKN"
     gene            8005..8796
                     /locus_tag="PK281_03705"
     CDS             8005..8796
                     /locus_tag="PK281_03705"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HOY48479.1"
                     /translation="MTFLEAFIIAVVEGLTEFLPVSSTGHMVITQALLGMDSDEFVKL
                     FTVNIQFGAILSVLLLYWKRFLQDIPFYIKLIIAFIPAGIIGLLAGDQIDLMLESPLV
                     VGITLLIGGVVLLYVDNWFKTEDSTTQITNQSALYIGLFQCIALIPGVSRSAATIIGG
                     MTQGLSRKTAAEFSFFLAVPTMAAASGYKLLKVFLKGDPTVITDNLSILLFGNVVAFI
                     VALLAIRFFIGFLTKHGFRLFGWYRIVVGAIILILLAAGVNLSVV"
     gene            8793..9494
                     /gene="truB"
                     /locus_tag="PK281_03710"
     CDS             8793..9494
                     /gene="truB"
                     /locus_tag="PK281_03710"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015481684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOY48480.1"
                     /translation="MSESPFHAGKLLLFDKPLGWTSFDLVNKVRFAIEKHYKLKRMSI
                     KVGHAGTLDPLATGLMLICTGKFTKQIDTFQGLPKTYTGTFTLGGTTVSFDLEKEVDQ
                     HFPTAHITEEMIYEVAKNFIGTHDQEPPVFSAKKIDGERAYELARAGKDVKMKTSSIE
                     ITAFEITRIALPEVDFRVECTKGTYIRSLARDFGLALKSGAYLSKLCRTAIGPHTIEN
                     AQTIEQFTAEIRSTV"
     gene            9897..10109
                     /locus_tag="PK281_03715"
     CDS             9897..10109
                     /locus_tag="PK281_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48481.1"
                     /translation="MSEDPTSNNQANDFQVPYNPDNSKRAGFVHESEDDRLLRDMNRP
                     PLEKLMLFTQMIRRERMFKQGKISNK"
     gene            complement(10195..11082)
                     /gene="atpG"
                     /locus_tag="PK281_03720"
     CDS             complement(10195..11082)
                     /gene="atpG"
                     /locus_tag="PK281_03720"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008236307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HOY48482.1"
                     /translation="MANLKEVRNRIVSVTSTMQITSAMKMVSAAKLRRAQDAIVQMRP
                     YAEKLKDILGNLSGSVDASEGGAYSVERDPKKVLIILITSNRGLAGAFNTNVLKIARQ
                     LITGKYAAQFAAGNVSFLCIGKKASDYVTRNKFNLYSSHNELFDRLAFDRVSPVAQRV
                     MDAFSNKEFDRVELAYNRFRNAAVQIPTEEQYLPVVRTEDAGTSKKNAAIDYIFEPDK
                     ATIVSELIPKSLKIQIYKAILDSHAAEHGARMTAMNKATDNARDLIKELKLSYNKARQ
                     AAITNEILEIVGGAEALNG"
     gene            complement(11101..12684)
                     /gene="atpA"
                     /locus_tag="PK281_03725"
     CDS             complement(11101..12684)
                     /gene="atpA"
                     /locus_tag="PK281_03725"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002681075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HOY48483.1"
                     /translation="MAEIKPAEVSAILKQQLSGFTTDVQLEEVGTVLQVGDGIARIYG
                     LNNVQAGELVEFPSGLQGIVLNLEEDNVGAVLLGSSNNIKEGDVVKRTRRIASINVGE
                     GMLGRVVNTLGEPIDGKGPIKGELLEMPLERKAPGVIYRQPVTEPLQTGIKAIDAMIP
                     IGRGQRELVIGDRQTGKTAVCIDTIINQKEFYEAGEPVFCIYVAIGQKGSTVANIVKT
                     LEENGAMAYTVIVSATASDPAPMQFYAPFAGVAIGEYFRDTGRPALIIYDDLSKQAVA
                     YREVSLLLRRPPGREAYPGDVFYLHSRLLERAAKINASDSIAQQMNDLPPSLQGKVKG
                     GGSLTALPIIETQAGDVSAYIPTNVISITDGQIFLESNLFNSGVRPAINVGISVSRVG
                     GNAQIKSMKKVAGTLKLDQAQFRELEAFAKFGSDLDAATKSVLDKGSRNVEILKQPQY
                     TPFKVEEQIAIIFAGVKGLLRPVPVNKVREFETDYLAILNTQHKDVLNALKAGKFEDS
                     LTDVLTKVANDLAARYTKN"
     gene            complement(12722..13282)
                     /gene="atpH"
                     /locus_tag="PK281_03730"
     CDS             complement(12722..13282)
                     /gene="atpH"
                     /locus_tag="PK281_03730"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HOY48484.1"
                     /translation="MQDSKAGIRYAKSLFNLAQDRNEVEAVSADMALIDKTIHENPEL
                     RSMLKSPIIKSDKKASIMRAIFTAHISTTTAAFLELIIKKRREMFMDSIARQFVILHL
                     ENNGIEEAVLTTAFKVDDSFRQRILDLVIKHSKNDKVVLEERIDPSIIGGFVLRFGDN
                     QIDTSVERDIELLRREFEKNLYVKEY"
     gene            complement(13290..13784)
                     /locus_tag="PK281_03735"
     CDS             complement(13290..13784)
                     /locus_tag="PK281_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HOY48485.1"
                     /translation="MKLVTPDFGLVIWMTLTFLTVLFLLSKFAWKPILKMISEREKTI
                     EDALLSAEKAKQEMARLTANNEALLREARSERDAMLKEARETKDQIINEARSRATVEA
                     ERLIAIARESINNEKMAAVTELKNQVAQLSLQVAEKLVKQQLSTDEKQKELANTLISE
                     LKLN"
     gene            complement(13865..14116)
                     /gene="atpE"
                     /locus_tag="PK281_03740"
     CDS             complement(13865..14116)
                     /gene="atpE"
                     /locus_tag="PK281_03740"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012821237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HOY48486.1"
                     /translation="MNAILLEAGNLGLGYGVAALGAGVAALGAGVGIGRIGGSALESI
                     ARQPEAAGDIRANMILTAALVEGAAFFAMVVGLLVTFKA"
     gene            complement(14152..15387)
                     /gene="atpB"
                     /locus_tag="PK281_03745"
     CDS             complement(14152..15387)
                     /gene="atpB"
                     /locus_tag="PK281_03745"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HOY48487.1"
                     /translation="MARSYSNSFLFSLLCSSLTLVFSLSFQPLSANAEEHGSAVQAAD
                     SGMLHQHTTTVGESSMAQAEHHVEADHAQGSHAFDPGHMITEHISDAHDWHLWGHTHV
                     PLPVILYTDKGIEVFSSANFRSHGEHGLNDYTGKHYTYRLEDEEVVILNAEGAIDEVA
                     TAGILDISITKNVLTMFIVFGLMLFLFISVAGAYKKRQGQAPKGLQSFIEPIIIFVRD
                     DLAKPSIGKKYEKFMPYLLTVFFFIWIANLLGLIPVLPGGANMTGNIAITLTLAVFSF
                     IITTINGNKHYWGHIFAMPGVPVWVLFLLTPIEILGVFLRPFVLMIRLFANITAGHII
                     ALAFFSLIFIFGKGGESVGAGVGVGVASWLFTVFMMFLELLVAFLQAYVFTLLSAIYF
                     GSAVEEAHHHDEHELEHAH"
     gene            15386..15571
                     /locus_tag="PK281_03750"
     CDS             15386..15571
                     /locus_tag="PK281_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48488.1"
                     /translation="MKEEVFNRGAKVGKEPTSKQFICMILKMIEYQPKEPHILHDLTI
                     LILKKKRNDLLSDHCVH"
     gene            complement(15610..>16410)
                     /locus_tag="PK281_03755"
     CDS             complement(15610..>16410)
                     /locus_tag="PK281_03755"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY48489.1"
                     /translation="LAYAWCMQKAPDNSGIVIGGGYTGTTQLGSFSLNGISQNLPFVA
                     KFDENGNYLNAFNYIQNLSQTDALSLSADENGNYYVGGRLTSSTMPVFSCTPAIGNRG
                     FYLGSFTEQPDTVPNPFITVDGDLLTASPTFMGNIQWYLNGNILPGENNQTLIATETG
                     NYSVEYAYLTGCVGADTSEVLEVIISSIASLVNENQIHLYPNPIQASGIITISGTYKN
                     DQLEITDIQGRLIKKVLLNETNQIDLAGIQSGLYLVKVQGKVSRLIIQ"
BASE COUNT         4688 a         3662 c         3182 g         4878 t
ORIGIN      
        1 gaatggttga aatcaatgct aagggtaaag tcaaattagc tgtagcaaat gccttgtgga
       61 atcgcttaaa gcttcatccc gattatttag caatcaccaa gaaatattat aacgcagaag
      121 tctatccatt aacgagcgaa acacccatta acgattgggc atccaagaac accaatggaa
      181 aaatccctaa agtgctcgaa cccggcgata ttacaccttt agtaaaattg atattaacga
      241 atgccattta tttcaaaggc gattgggtga aaactttcga taaggagaat acgcaagaag
      301 ccaagtttta tcagagcaat aaaagcattt caccatgcag tttaatgtac gcaaaagact
      361 attataggta tggtcaattt gatggttatc aagccattga gcttccttat tcaggagatg
      421 atttaagcat ggttgtgtta ttacctaaga gagaaagctc cttaaagcag ctctttcaaa
      481 cgttgaatac gaaagaactc gatactcaat tacgagcatt aagaagccaa caagtggaac
      541 tctggctgcc aaaattcaaa ctcgaaacct cctacgaact caaagatgta ttgatgcaaa
      601 tggggatgaa agcacctttt agccgtgccg attttactaa gatgaccaaa gacaaaacac
      661 cattagaaat cagccgcatt attcaaaaag catttgttga agtaaatgaa aaaggtaccg
      721 aagccgcagc agttacagtt gtgatgatgg ctacggagtc gagtataagc agacatgaaa
      781 tagaaattcc acaattcaga gccgatcgac cattcttatt cctcattcgg gataaacaaa
      841 ctggagcgat actatttact ggaggattgg aaaaacctta agtgtagttt tcaaatcaat
      901 tggttgaaat tcattaattt ggcaaatgcg aaatttactt ttcacgtttg ccttttttat
      961 gctctcaatt gaggcaatag tcgcacaggt aaactacaac gacgatataa caatcagtct
     1021 aacaggagct atcagcgcac aaaatccggg ttggcctttt aatggtccat ataatttggt
     1081 tccaagtggt aacctaaatg gtttcatctt ctccggttca actccaacat gttctgctag
     1141 ttcctatcat acgtttggca actttggctc tggttggact actggacagg cgcagatttc
     1201 tcaaccaagt ggctggaatt acgaagccta tgtggatttt gggcatactg cctcttggcg
     1261 ttgtaatgtt ccattagttg gttcaacagt aaatacatcc tacgcagcca ttcatggggt
     1321 tgttattggt ggagcactta ttctcaaaac gacatacgga aatacatctc acgacgattt
     1381 acgggaatac atgtatgtga aattggcgcg tccgctgatg catggaaaaa cctatcattt
     1441 tcgatacacc gttgcagctg cacatccaag cggagaacta acaacagcca atgtttggca
     1501 agatcaatta ggtgtttatt taagtcccaa tcctcccaat tgggctggtg cgtataccaa
     1561 tgttcctgtt gatacaatca cacctacatc cgtcactcct gctggacaaa taatttctga
     1621 cactggaata accattgaac aggattttat tggcaatgga gaagagtggt tgatggttgg
     1681 gagtttcagt ccgctcgatt ccattcacta ttcaggtcct cacgatgtga cgtttcagtc
     1741 gcgctaccta ttcgacaatt ttgccattta cgacccgaat tgcgaaaacc cgatatacct
     1801 cgtattgcct caacctccaa caactccata ttgtcatcca acaaatccta tttcttgcta
     1861 ttcaacaaca ggttttgacg attatcgctg gtatgtagat ggcgttttgc aaagcgatac
     1921 ttcgtatacg tttaactata ttccacaaca tagcacaacc ataagtcttg caggaacaat
     1981 aggcacatgc gtcgattcta tcagctattt ctatcctgtt caggaggtag attttggact
     2041 tcctgccgat atatttctct tttgtggcga tccaataaca cttgatccag aaattcaaac
     2101 caattatccg attcaatatt ccgaatggac caatctggca aataatcagg tttttaatgg
     2161 ttcaggacct tatacgattt ctcaatacgg cgattatgaa gtgcattcca tgaccatttc
     2221 gggatgtaat ttcagagaca cattgcaggt gattcctcgc ttattaccta caacaaatac
     2281 acaaggtcaa gcgattcctg taatccaaat gcgcgatgta agttgcccgg gtgaggatga
     2341 tggttatgcg atttataatt tcccgggatt ggattctttg cattacgact ggggaattgc
     2401 aggcgtatca gatacttcaa caggtatata taattctcca gcacagaatt ttaatttacg
     2461 cgtttacgat aatgaccagc attgcacggt tttctatcct tccatcatca ataatttcga
     2521 tagttgtgcg gtgataaatg gaacaattta cctggatggg gatgcagatt gtttgttgga
     2581 cacaaatgag cttactgttt catcagtaat gatgtattct ccagaatttg atcgaatggc
     2641 cctgactgat tccatgggaa attacacgtt gagaattcca cccggtccac aacatattct
     2701 tcaaagcagt tacaaccatc ctttgatggg aacactctgt gaggatacgg ttttggtcaa
     2761 cccaacagca ttcaaccaaa tcattaacgg aattaatttc ggagatacga ttactgctcc
     2821 tgtttacgat gctgccattg tatttggagg atgtgcgcaa ttggttccgg ggcatattag
     2881 ctatatccct tcagtagtgc agaatacggg aaatctccct atcagttttg tgtttcgtca
     2941 ggttatcagt gattcacttt ggatagatca tgcagaaggt tatttctatg gcacttggga
     3001 tgaaattgct ttacaaggta taaacgcaga taccgcaaca tggcagttag atttagcacc
     3061 aggacaatcg gcctatttta gaacttgggt agaaatacca cccaatcctg ctttggtggg
     3121 aagtactttg cattcctaca gtgaaattat tccactgctt caggatgatg attccgacaa
     3181 taatcattta gatatgaatt gtttggtgat tggaagtttc gatccaaacc gaaaaaatgt
     3241 gaatcctgca gggcaaggtg agcaagggtt gattagtgct gaggttgaag attttcaatt
     3301 cctcatcgaa tttcaaaata ctggaacagc acccgctgat aatatttatg tagaagacac
     3361 actttcttct cttttcaatc tgaattcgct tcaagtgatg ggggcaagtc atgcttatca
     3421 aaccgaaatt cgtcaaggaa atctcatccg tttcattttc ccgaacatca acttagccga
     3481 ttcacttagc aatgaacccg aatctcatgg ctggattgcg tttaaagtga aacgtcaagc
     3541 taatgcaacc aatccgggaa cagaattcca aaatacagca ctcatttatt tcgatttcaa
     3601 cgaaccggta gtaaccaata ccacgctgaa tacgattgcc gaagatgtag tattgagtgt
     3661 tcctcaaaaa gaaacagctg aattagaaat ctatcctaat ccagctgtaa atactattcg
     3721 tattctattg ccatccaaag cgaaggaaat ccgaatcagc gatatctctg gaagagtgct
     3781 tcttcgtaaa acgggcgatg cgaatctgct gcatcaattg gatattcaat ccttgtcgag
     3841 cggaatgtat cttgttgacg taacacttaa caatggctca atcgttcaaa agaagttgat
     3901 cattcgttaa ttttaaatca ccacgttcac cattcttccc ggaacaacaa tcactttctt
     3961 tggaccgcct tctggtagcc atttagcagc atcttcatga cttaatgcta aagcttgaac
     4021 ttcggcttga ggcatatcag cagctacctg aatttggtaa cgtacttttc cattgaaagc
     4081 tactggataa gcgattgaac tttctttgat gaagctttca ttccattgtg gaaattgagc
     4141 atctacaacc gatccagatc cgccgcatgc ttgccacaac tcttcagcca tatgaggtgc
     4201 atacggtgct ataataacgc tcaagtcctg aagaatagct cgtttgcgac acttttgttc
     4261 acttagttca tttacacaaa tcataaacac actcaccgaa gtattgaaag agaatttctc
     4321 aatgtcttct tctactttac gaatggtttt atgcaaggtg cgcaactctg cttctgtagg
     4381 cgcttcatcg ctcagtttca cttgtccatt ctcatcgaag tacaaattcc acagtttttt
     4441 caggaaacga taaactcctt caattccatt ggtattccaa ggtttgaatt gctccaatgg
     4501 tcccaaaaac atctcatata agcgcaaagt atccgcacca taatcattga tgatatcgtc
     4561 gggattaacc acattgtact tcgacttcga cattttctca acctctacac cgcacacata
     4621 attgccttct gcattcaaga taaattcagc tgctgcattt tcatcccgcc atgctttaaa
     4681 agcgttgagg ttcaataaat cattctgcac aatgttcaca tccacatgag tttgcaggat
     4741 tttttcatcc ttgaattcgc cgagcatatc tttcgaaatg aacttggttg ttccaataat
     4801 ttgataaaca aaattcgaac gccccaaaat catcccctga ttaatcaact tgcggaaagg
     4861 ctcgttttta ctcacttccc cacgatcaaa caagaatttc tgccagaagc gcgcgtacaa
     4921 taaatgtcca actgcgtgct ctgttccgcc aatgtataaa tccacatctt gccaatattc
     4981 ttcagcttct cttcctacaa aacgagcttt gttttgcgga tccatatagc gcaagaaata
     5041 ccaagaagaa cctgcataac ccggcatagt atccgtttca cgtgttccat ccggtacatt
     5101 cacccattct ggcactttgg tcaaaggact ttcaggctgt cccgtcggtt tgaaatcggc
     5161 catcactggc aattccaaag gcaattcgtt ttcagaaagc ggatacgcaa caccatcgcg
     5221 atacacaatt gggaagggct ctccccaata acgttggcga ctaaatcctg catcacgcaa
     5281 gcgataattc actttacctt ttccaaatcc acgctcttca atagcagcaa ttgctttctt
     5341 caaggcttcg tatccagtta atccattgag gaaatcagag ttaatcatct tggcttcctt
     5401 ggtaggatta gccgcttcag aaatatcaac tccttcgttt attacaggaa taggaaggtt
     5461 gaaatgttta gcaaaatccc aatcgcgttg atcgccagca ggaacagcca tcaccgctcc
     5521 ggtcccatat cctgccaaca cataatcgcc cacccatacc ggaattttgg ctcctgtaaa
     5581 tggatgaagt acatacgctc cggtgaattc cccgctgatg cgtttcacat ctgccattcg
     5641 ctcacgttcg cttcggtttt tagcaacttc cacgtaagca ttcacggcat cgcgttgcaa
     5701 gtcagttgtg ataacagaca ccaattcatg ttcgggtgca agggttaaaa agctaactcc
     5761 aaaaatggta tcagggcgtg tggtgaagac ttcaatctga tcgggatgat tttccaattg
     5821 gaatttcaaa gaagctcctt ccgatttccc aatccagttg cgctgcattt ccttcatcga
     5881 atcggtccac tcgagtgttt ccaaatcgtt gagtaatctt tctgcatagg cagtaatgcg
     5941 aaggaaccat tggcgcatac ggcgtttttc aacgggatgt ccgccacgtt cgctcaagcc
     6001 atcttttact tcatcgtttg ccaacacagt tcccaaagct tcacaccagt tcacggtgct
     6061 aaaatcttgg taagccaaac ggtagtgcat caaaattcct cgctgatcgg cctcgttcat
     6121 ggcattccat tcagcagcac taaatggggc atgttctcca caagctcctt ttgcagcagt
     6181 acttcctcct tgggaaaaca atgcttcaag cttggaaatg tcttctgctt tctgactttc
     6241 taaattgaac cagcagccaa acaattgaag gaaaatccat tgtgtccatt tgtgatagtt
     6301 tggatcagaa gtaattactt cacggtccca atcgtaacaa aatccaatgt ttccaagctg
     6361 ttctttataa cgggcaatgt tttcggcagt cgtatcggct ggatgacgac ctgtttgaat
     6421 ggcatactgc tctgcaggta aaccaaatgc atcgaaaccc attggatgca atacattaaa
     6481 tcccttcaat cgcttgtaac gcgaaacaat atccgaagcg atatatccga ggggatgtcc
     6541 cacatgtaat cctgcacccg atgggtaagg aaacatgtcg agtacataat acttgggctt
     6601 agaaagatcg atgttcgtct tataacgctt ctcatctttc caggtttgtt gccatttttt
     6661 ttcaatcgcg cggaaattcg gctccatcag ggaatcgggg tattttaatg aagcggcgaa
     6721 gttacaaaag cacacgtgaa acttcattac aagttcattt cctcacactt gtccacattt
     6781 tctgttcatc atcctgttga aagatgacca aaacacttat tcagttcgcc tttagcatct
     6841 gtatatttgg cctttgtttc ctagtacatg tccagtaacg aaaatcagtt caaccgccgt
     6901 cgaattcgcg cctcctacat cacttctgtg gtgagtatcg ccatggtatt gttcatgctc
     6961 ggtttgctcg gactgctcct cgtcgatgcc aaaaaacttt ccgacaccac acgtgaatcg
     7021 tttcaggttt cggttttctt tcgcgattcg gttgacgatg ctcaaatcct agcattcaaa
     7081 caagaactcg atacacagcc ttacgtaaag tcgaccactt ttatcacgcg tgctcaagct
     7141 gcagatgaat ttcagaaaga tttgggtgaa gagtttgtcg atttccttgg atacaatcca
     7201 cttccggctt ctattgatgt gcgattcaaa gtgaatttca cgaacgaaag cgattttcaa
     7261 aaaattgaaa aggaatggct ggcaaatcca ctcgttgctc aggttgatta tccacggaat
     7321 ctaatcaacc aaatcatcga taatatgcga aagttgagtt ggtttttcct cgctttctca
     7381 gctattctaa catttattgc ggtaacgctg atcaacaata ccatccgact ttcaatttac
     7441 tccaagcgac tgctcattcg aaccatgcag cttgttggag ctactcgtgg ttttattcgc
     7501 ggaccatttc tccgagctag tttcatgcag ggttttgcag gtggaatttt agctatactc
     7561 ttattatcag gaatgctgta tttgggcgaa caacaaatcc ctgaactgaa gcctctgcgc
     7621 gactttaact tacttggtat tttagccgga gggatacttc tggggggtat cggattgtcg
     7681 tggatttgta ctttctttgc agtccgtaaa tacttgcatc tcaaaacaga ttcactgtat
     7741 taagccttac agatgagcac cgaaaaagac aacaatcaga aagggtttgc attcagcccg
     7801 atgaattata aaatcatgct tgccggaatt ctagtaattc tagttggttt cctgctcatg
     7861 gttggcggag gttctaaaga tcctaatgtc tttaatcccg aaatctttag tacacaacgt
     7921 atcactgttg ccccaatcgt agtgctgatt ggtttcgcaa tcgaagtgtt cgcgattctc
     7981 tacaaaccaa aaaactaatt ccggatgact tttctggaag ccttcatcat tgcggtggtg
     8041 gaaggactta ctgagttttt acccgtgtcc tctactggtc acatggtcat tactcaggct
     8101 ttgctcggca tggattcaga tgaattcgtg aaactcttta ctgttaacat tcagtttgga
     8161 gccattcttt cggtcctcct tctttattgg aaacgttttc ttcaagacat tcctttctat
     8221 atcaagctga ttatcgcttt tattcctgct ggtatcattg gattattggc aggagatcaa
     8281 atagacttaa tgctcgaaag tccattggtt gttggtatca ctcttttaat tggaggtgtt
     8341 gttttgttgt atgtcgataa ttggtttaaa accgaagatt caactacaca aatcaccaat
     8401 caatcagcac tatatattgg gcttttccag tgtattgctc tcattccagg cgtttctcgt
     8461 tcggcagcta ccattattgg aggaatgact caaggtcttt cacgtaaaac agcagcagaa
     8521 ttttcttttt tccttgctgt acctactatg gctgcagcat ctggatacaa attgctcaaa
     8581 gtttttctta aaggtgatcc aacagtcata accgataatc ttagcattct tcttttcggg
     8641 aatgtggtag catttatcgt tgctttgttg gctatccgct tctttattgg cttcctaact
     8701 aaacacggat ttcgtctttt tggatggtat cgtattgtag taggcgctat cattttaatt
     8761 ctgctcgctg caggagtcaa tctttctgta gtatgagtga atctccgttc cacgctggga
     8821 aacttcttct tttcgacaaa ccactcggct ggacttcttt cgacctcgta aataaagtcc
     8881 gttttgccat cgaaaagcat tacaagctaa aacgtatgtc gataaaagtt ggacatgccg
     8941 gcacgctcga tcctttggct actggcttga tgctaatttg caccggtaaa ttcaccaagc
     9001 aaatcgatac atttcaaggt ttacctaaaa cctatacggg cacgttcaca ctcggcggga
     9061 cgaccgtttc tttcgattta gaaaaggaag tagatcaaca ttttcccaca gcgcacatta
     9121 ctgaggaaat gatctatgaa gtagcaaaaa acttcatcgg aacacatgac caagaaccgc
     9181 ctgtattctc cgctaagaag attgatggcg aacgtgctta tgaactagct cgcgctggga
     9241 aagatgtgaa aatgaaaact tccagcatcg aaatcaccgc attcgaaatc acccgaattg
     9301 cccttcctga agttgatttc cgtgtagaat gtaccaaagg aacttatatc cgttcgcttg
     9361 cccgcgactt tggactagca ctaaaatcag gagcctactt atccaaactt tgccgcacgg
     9421 ctattggtcc acatacaatc gaaaatgctc aaactatcga gcaattcaca gccgaaattc
     9481 gttctactgt gtaagccgaa gctttttaag cgaaggctta ccgttgaagc ttaatttttt
     9541 gtctccttcg ctctctatat ctcgtaagcc gaagctttaa gcgaaggctt acagaccaag
     9601 tttagttttt tgtctcctta gctctctatt tctcgtaagc cgaagctttg agcgaaggct
     9661 tattagcgaa ggcttacgtt gccgcttttt ctaagaatga ttatttattt aacactacga
     9721 tataaagttt attcgtggaa ttcaaaatca tgcccctcct gccatatagt ggtccctacg
     9781 ggaaggagag ggaaggggtg aggtcaacgt gctttttcta atttggagcg ttttctcttt
     9841 ctatgagaaa cccacccatt cgtttaagta catattaatt cgtaatttgt actatcatga
     9901 gcgaagatcc aacatcaaac aatcaggcta acgattttca ggttccctac aaccctgata
     9961 actcaaagcg tgcaggattt gttcatgaat cagaagatga ccgcctactc cgtgatatga
    10021 atcgccctcc attagaaaag ctcatgttat ttacacaaat gatccggcga gagcgaatgt
    10081 ttaagcaagg gaagatttct aacaagtagt acatcgaaat ccttaaaaat tccccaataa
    10141 aaaaacgcat ccccttttca gaagatgcgt tctcaatata gttttgattg atgcttagcc
    10201 gttcagtgct tctgctccac caacaatctc caagatttcg ttggtaatag ctgcctgacg
    10261 tgctttgttg taactcaatt tcaattcttt gatcaaatca cgtgcgttat cggttgcttt
    10321 gttcatcgcc gtcatacgtg caccatgctc agctgcgtgt gagtcgagga tggctttgta
    10381 aatttgaatc ttcaacgatt tcggaatcaa ctcgctaaca atagtcgctt tatctggctc
    10441 gaaaatgtaa tcgatagctg cgttcttttt gctcgttcca gcatcctcag tgcgaacaac
    10501 aggtaaatat tgctcttcag taggaatttg aaccgcagca ttacggaaac gattgtaagc
    10561 caattcaacg cgatcaaatt ctttattcga aaatgcatcc attacacgtt gtgcaacagg
    10621 tgatactcga tcgaatgcca aacgatcaaa taattcgttg tgcgaactat ataagttgaa
    10681 tttgttacgt gttacgtaat cgcttgcttt ctttccgata catagaaacg aaacatttcc
    10741 agcagcaaat tgagcagcat atttaccagt gattaactga cgagcaatct tcaatacgtt
    10801 cgtattaaat gctcctgcca gaccacggtt cgacgtgatc aaaatgatca gaactttttt
    10861 cggatcacgc tctacagaat atgcgccgcc ttcacttgca tcaacacttc cgctgaggtt
    10921 acccaggata tctttcagct tctccgcata aggacgcatc tgcacaatag catcttgcgc
    10981 ccggcgtaac tttgcagccg ataccatttt catggcactc gtaatctgca tcgtggaagt
    11041 taccgaaaca atccggttac ggacttcttt aagattggcc atcggaccgg ttttgaatga
    11101 ttaattcttg gtataacgag ccgccaaatc gttggctact ttagtcaata catctgttaa
    11161 gctatcttcg aatttcccag ctttcaatgc atttaaaaca tctttatgct gcgtgttcaa
    11221 aatagctaag tagtcagttt caaactcacg aactttattc actggaactg gacgaagaag
    11281 tccttttaca ccagcgaaaa tgatcgcaat ctgttcttct actttaaatg gagtgtactg
    11341 aggttgtttc aagatttcca cgttacggga acctttatcc aataccgatt tagtagctgc
    11401 atctagatcc gaaccgaact tagcaaacgc ttccaactca cggaactgcg cttggtcaag
    11461 tttcaatgtt ccagctactt ttttcatcga cttaatctgc gcattaccac caacacgtga
    11521 taccgaaata cctacgttaa ttgccggacg aacacctgag ttgaacaagt tcgactccaa
    11581 gaaaatctgt ccgtcggtaa tagaaattac gttcgttggg atgtatgcag aaacgtcacc
    11641 cgcttgggtt tcgataattg gaagtgctgt taatgaacca ccacctttta cttttccttg
    11701 aagagaagga ggaaggtcat tcatctgctg cgcgatagaa tcggaagcat tgatttttgc
    11761 agcacgctca agtaagcgac tgtgtaggta gaatacgtct cctggatatg cctcacgtcc
    11821 tggtggacga cgaagtagaa gagaaacctc gcggtaagct acggcttgtt tagaaagatc
    11881 atcgtaaatg atcaatgccg gacgacctgt atcacggaag tactcaccaa ttgcaacacc
    11941 tgcaaatggt gcatagaatt gcattggagc aggatcagaa gctgttgcag aaacaatcac
    12001 ggtgtaagcc atcgcgccgt tttcttccaa tgttttcaca atgttcgcaa cagttgaacc
    12061 tttttgtcca atcgcaacat aaatacagaa tacaggctca cctgcttcgt agaattcttt
    12121 ttggttgatg atggtatcga tacacactgc tgttttacca gtctgacggt caccgataac
    12181 caactcacgc tgaccacgtc caattggaat catcgcatca atcgctttga tacctgtttg
    12241 aagtggttca gtaaccggct gacgataaat tacccctgga gctttgcgct ctagtggcat
    12301 ttcaagaagt tcgcctttga ttggtccttt accgtcgatt ggttcaccta gtgtattcac
    12361 tacgcgacca agcatgcctt caccaacatt gatggaagca atacggcgag tacgttttac
    12421 aacgtctcct tctttaatgt tgttggaact acccagcaat acagctccta cgttatcttc
    12481 ttctaagttc aatacgatgc cttgaagtcc gctagggaat tcaacaagct ctcctgcttg
    12541 aacattgtta agaccgtaga tgcgggcaat accgtctcct acttggagaa cagttcccac
    12601 ttcttcgagt tgcacgtcgg tggtaaaacc agacagctgt tgtttcagga tagcagatac
    12661 ttctgcgggt ttgatttcag ccattgttcg tgttatgagg gtgtgacttt cgccacggtt
    12721 ttcaatactc ttttacgtaa aggttctttt caaattcgcg gcgcaacaac tcaatgtcac
    12781 gctcaacgct agtgtctatt tggttgtcac caaaacggag aacaaatcct ccgatgatgc
    12841 ttggatcgat gcgctcttcc aaaacaactt tgtcgttctt agaatgctta atcaccaaat
    12901 ccaatatacg ttgacggaaa ctatcatcca ctttaaatgc agtagtaaga acagcttctt
    12961 caattccgtt gttttccaaa tgaaggatta caaactgacg ggcaatcgaa tccataaaca
    13021 tttcgcgacg tttcttgatg attagttcaa gaaatgcagc ggtagtcgta ctaatgtgtg
    13081 cagtaaaaat agcacgcata atagatgctt tcttatccga tttgataatc gggcttttca
    13141 gcatcgaacg tagttccggg ttttcgtgaa tggttttgtc gattagtgcc atatcagcac
    13201 ttacagcctc cacttcattg cgatcttgcg ctaaattgaa gagagacttc gcgtatcgta
    13261 tgcctgcttt cgagtcctgc atggttcgat cagttcaatt tcaattctga gatcaaggta
    13321 ttggcaagct ctttctgttt ctcgtcggtt gataattgct gttttaccag cttctcagcc
    13381 acttgaagag aaagttgagc aacctgattt ttcaattcag tcacagcagc cattttctca
    13441 ttgttaatcg actcacgtgc aatggcaatt aagcgttctg cttcaactgt tgctcttgat
    13501 cgtgcttcgt taatgatttg atcttttgtc tcgcgtgctt ctttcaacat cgcatcacgc
    13561 tccgaacgag cttcacgtaa taatgcttcg ttgtttgctg ttaagcgagc catctcttgt
    13621 tttgcttttt cagcgctgag taacgcatct tcgatggtct tttcgcgttc gctgatcatt
    13681 ttaagaatcg gcttccaagc gaatttactc agtaaaaaaa gcaccgtcaa aaaggtcaag
    13741 gtcatccaaa tgaccaaacc aaaatcgggg gtgactaatt tcatatatcg tgtgttttga
    13801 atttcgtttt gaaaaacctg tcagatagcc aaccgcttcc gacaggttaa ttttctgaaa
    13861 ctacttatgc tttgaaagtc acaagtagac ctacaaccat cgcgaagaag gcagcacctt
    13921 caacaagagc agctgtaaga atcatgttag cacggatatc acctgcagcc tcaggctgac
    13981 gagcgattga ttccaatgcg ctaccaccaa tgcggccaat accaacacca gcaccaagag
    14041 ctgcaacacc agcaccaaga gcagcaacac cgtaaccaag tcctaggttt ccagcttcca
    14101 aaagaattgc gttcataaaa ttgagatata aggattagaa aaattcaaga attaatgagc
    14161 gtgttccaat tcgtgttcat cgtgatgatg agcttcttcc acagcagaac caaagtagat
    14221 agccgaaagt agcgtaaata cataagcctg aaggaaagca actaacaact caaggaacat
    14281 cataaatacc gtgaataacc agctagctac accaacgcca acacctgccc caaccgattc
    14341 tcctccttta ccaaagatga aaatcaaact gaagaatgcc aatgcgataa tgtgacccgc
    14401 agtgatgttc gcgaaaagtc ggatcatcaa tacaaacgga cgaaggaata cacctagaat
    14461 ttcaattggt gttaaaagaa acaataccca aactggcaca cctggcatcg cgaagatgtg
    14521 tccccaataa tgtttgtttc cattaatagt agtaataatg aaactgaata ctgccaatgt
    14581 taaagtaatg gcaatgttac cagtcatgtt tgctccaccg ggaagtactg gaatcaaacc
    14641 taataggttg gcaatccaaa tgaagaagaa tacagtaagt aaatacggca taaacttctc
    14701 gtatttcttc ccaatacttg gtttagctaa atcatcacga acaaatatga taataggctc
    14761 gataaaactt tgtaaacctt ttggggcttg accttgacgt tttttgtatg ctccagctac
    14821 tgaaataaat aggaacaaca tcaatccaaa tacgatgaac atggtaagca cattcttcgt
    14881 gatggaaata tctaaaatac ctgcagttgc aacttcgtca attgctcctt ccgcatttaa
    14941 aatcactact tcctcatctt ccaaacgata tgtgtagtgc tttccagtat agtcgttcaa
    15001 accatgctct ccatgactac ggaaattagc agaagaaaaa acctctattc ctttatctgt
    15061 atacaatatt acaggtagag gaacatgagt atgtccccat aaatgccaat cgtgagcatc
    15121 actaatgtgc tcagtaatca tatgaccagg gtcaaatgca tgagaacctt gagcatgatc
    15181 tgcttctaca tgatgctcag cctgagccat gctactttct ccaacagtag ttgtatgttg
    15241 atgtaacata cccgaatcgg cagcctgtac ggcacttccg tgttcttctg cattagccga
    15301 caatggctga aatgacagag aaaacaccaa agtcagtgat gaacaaagga gggaaaagag
    15361 gaaagaatta ctgtagcttc tagccatgaa agaagaggtc tttaatcgcg gtgcaaaagt
    15421 aggaaaagaa ccaacatcca aacagtttat ttgtatgatt ttaaaaatga ttgaatatca
    15481 accgaaagaa ccccatattc tccatgattt gaccatctta attttgaaaa aaaaacgcaa
    15541 tgatctgctt tccgatcatt gcgttcatta ataatgggtg tcgaacaggt tgtgttttct
    15601 agctttggat tattgaatta tcaagcgtga aacttttcct tgaactttta ccaaatataa
    15661 tcccgactga attccagcta gatcgatttg attcgtttca tttaacaata ctttctttat
    15721 caatcttcct tgtatatcag taatttcaag ttgatcattt ttatatgtac ctgaaatagt
    15781 aattataccc gaagcttgaa ttggattcgg atataaatga atttggtttt cattaacgag
    15841 tgatgcaata cttgaaataa ttacctctag aacttccgat gtgtcagctc caacacatcc
    15901 ggtgaggtaa gcatattcaa cactatagtt gcctgtctcg gtagcaatta atgtttgatt
    15961 gttttctcca gggaggatgt ttccatttaa gtaccactga atatttccca tgaatgtagg
    16021 tgatgctgtc aacaaatctc catctactgt tataaaagga ttcggaacag tatcgggttg
    16081 ttctgtaaaa ctccccaaat aaaaacctct attccctata gcgggtgtac aactaaacac
    16141 cggcatagta ctgctagtta atctccctcc aacataataa tttccattct catcggccga
    16201 caagctaagt gcatctgttt gacttaagtt ttgaatgtag ttgaacgcat tcaggtaatt
    16261 tccattttca tcaaacttgg ctacaaaggg caggttctgg cttattccgt ttaatgaaaa
    16321 ggatcctaat tgggtagttc cggtatatcc accacctatt acaataccgc tgttatcagg
    16381 tgccttttgc atacaccatg cataagccaa
//
LOCUS       DAOOQW010000035        16346 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_4322_length_16346_cov_5.362040, whole genome shotgun sequence.
ACCESSION   DAOOQW010000035 DAOOQW010000000
VERSION     DAOOQW010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 16346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16346
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4322_length_16346_cov_5.362040"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..968)
                     /gene="sufB"
                     /locus_tag="PK281_03760"
     CDS             complement(<1..968)
                     /gene="sufB"
                     /locus_tag="PK281_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HOY48490.1"
                     /translation="MAAEGNEILESHINSDYKWGFTTDIEMDSAPKGLNEDIIRFISA
                     KKKEPEWMLAYRLKAFRYWLTLEEPVWAHVNYPKIDFQDIIYYAAPKKTKQLNSLDEV
                     DPELRKTFEKLGISLLEQKRLTGVAVDAVIDSVSVKTTFKEKLGELGIIFCSFSEAVH
                     DHPELIKKYMGSVVPYTDNYYAALNSAVFTDGSFCYIPKGVRCPMELSTYFRINASGT
                     GQFERTLIVAEEGAYVSYLEGCTAPMRDENQLHAAVVEIIAEERGEVKYSTVQNWYPG
                     DKNGVGGIFNFVTKRGICAGFGAKISWTQVETGSAITWKYPSVILKG"
     gene            complement(980..1306)
                     /locus_tag="PK281_03765"
     CDS             complement(980..1306)
                     /locus_tag="PK281_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007646870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HOY48491.1"
                     /translation="MISVSDTAKEKLLSLMAESNHSADSYLRVGVEGGGCSGLSYKMD
                     FDAERRDGDQLFEDKGVKILVDKKSFLYLVGTVLEYSGGLNGKGFSFNNPNATRTCGC
                     GESFAV"
     gene            1535..>2139
                     /locus_tag="PK281_03770"
     CDS             1535..>2139
                     /locus_tag="PK281_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48492.1"
                     /translation="MGTEMMPQYEETEEKIVNHLEEWILATNRSPINKSVGGFLRSAS
                     PALNILAKTRVAAFVYDYENGSYSYFNDYFPILLNSTRRHIEKVGIRVMEERVHPEDF
                     IKCLNLTRRTLEEFSKMKEDEKDSTQLRLFFRVKRKSGDYVWVLQSNRQVRWSKDAPA
                     LDLAYMMELFDSHNPMKVMAVLQTSRRVMEFLPDGETELLS"
     assembly_gap    2140..2239
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2240..2748)
                     /locus_tag="PK281_03775"
     CDS             complement(<2240..2748)
                     /locus_tag="PK281_03775"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOY48493.1"
                     /translation="MRLLLFTLVSFVVTTMMAQEPIRFLALGDSYTIGESVAEQDRWP
                     VQLSRLMHDAGVNLNQPEIIATTGWTTDELSKAIDDRKPGNDYGLVSLLIGVNNQYRG
                     NSQEVYVQEFTALLNRAIEFAGGIKNHVVVVSIPDWGVTPFAADRDRDKIAEEIDAFN
                     AINRQISES"
     gene            complement(2748..4226)
                     /gene="rpoN"
                     /locus_tag="PK281_03780"
     CDS             complement(2748..4226)
                     /gene="rpoN"
                     /locus_tag="PK281_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010230255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HOY48494.1"
                     /translation="MLKQGLQQKLLQKLSPQQIQLMKLLQVPTVSLEQRIKEELEINP
                     ALEEGAEADDTKEETSEDPFSSTDDGDVEAHSEQNREDFDLGAYIDDDETPAYKLAAN
                     NYSPDDERKEIPFAGGQGFQEQLLSQLGLRNVSEEEFPIAEYLIGNIDEDGYLRRDIS
                     AIVDDLAFSQNILTSEEDVLRLLREVQELDPPGIGARDLQECLLIQLRRKEHQTVEMK
                     TAAVILRECIDEISRKHYDKIAKKLELTDEELKAAIKEIMKLNPRPGNSQSDSPKSTQ
                     HIIPDFLLNNNEGVLELQLNSRNAPDLRISRTYQEMLVHYSKTKKVSDKEQRDAVLFV
                     KQKIDSARWFIDAIKQRQQTLMLTMQAILDYQYEYFLTGDETKLRPMILKDIAEKVFL
                     DISTISRVANSKYIQTSFGTFLLKSFFSESLSTDTGEEVSTREVKKILEECVAAEDKS
                     KPVTDDKLAKVLREKGYNIARRTVAKYREQLNIPVARMRKEL"
     gene            4471..5115
                     /locus_tag="PK281_03785"
     CDS             4471..5115
                     /locus_tag="PK281_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48495.1"
                     /translation="MKPGKNQFFKPLTSLFGISFLFLAGLCFIANPSFAANSTLTQHL
                     KYTVYVSGSKRGTLQADINRKPDKSFDIRLETKLHFPFTDVVSLIQVHYTAHGLESSS
                     SLKHINDDLKEKITVKRVDGGYALTDVDGKNTQLLGKIPFSVGRLYHDEPVGMKELFS
                     ERLGTFVPIRSLKAGEYELLQPDGKKNVFVYRYGICTEMRTEMMACNVRFVLTD"
     gene            complement(5112..6548)
                     /gene="asnS"
                     /locus_tag="PK281_03790"
     CDS             complement(5112..6548)
                     /gene="asnS"
                     /locus_tag="PK281_03790"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010517696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HOY48496.1"
                     /translation="MSALHQKRIADWLKTQPENQEITVSGWVRTKRGNKDIAFIALSD
                     GSTINNLQIVADLSKFDEELLKKVQTGCSLRVTGNLVASQGAGQAVDLSATKIEVLGE
                     ADAEKFPLQKKKHTLEFLREIAHLRPRTNTFGAILRIRHALAFAIHKYFNDNHFYYIH
                     APIITGSDAEGAGEMFRVTTLDAANPPRNEDGSIDSTQDFFGKDANLTVSGQLESELA
                     AMALSRVYTFGPTFRAENSNTTRHLAEFWMIEPEMAFFELEDNMDLAEDFLKYLIRYA
                     MEHCADDLAFLEKRLIEEESQMKKEDRNPMALLEKLNFVAEQPFERLTYTEAISILER
                     SGKKFEYPVKWGVDLQSEHERYLVEKHFKKPVILTNYPKDIKAFYMKQDEDGKTVRAM
                     DVLFPGIGEIIGGSQREEDYEKLSRRIDEMKIHRESLWWYLETRQYGTVPHSGFGLGF
                     ERLVLFVTGMGNIRDVIPFPRTPKNLEF"
     gene            complement(6603..7124)
                     /locus_tag="PK281_03795"
     CDS             complement(6603..7124)
                     /locus_tag="PK281_03795"
                     /inference="COORDINATES: protein motif:HMM:NF009807.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal-dependent hydrolase"
                     /protein_id="HOY48497.1"
                     /translation="MDNLEHLRFPIGKLVVPETYPMADIKSWIENIRSLPQEIRAVAN
                     SLSEEQLAKPYRPEGWTGKQVIHHVADSHLNCLVRVKLALTEDNPTIKPYNEEKWARL
                     SDYEMPISISLDLIENTHAHLVYIFDRMTELDFHRMFTHPQYQYTRSIGYLCALYSWH
                     GKHHTAHLKLLQG"
     gene            complement(7117..7632)
                     /locus_tag="PK281_03800"
     CDS             complement(7117..7632)
                     /locus_tag="PK281_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48498.1"
                     /translation="MKSRIYLSLFAGMTLMACGGPSVKPEVYNDSLVIQQIRVIEKAD
                     ELQDAFNSYVEAEMRMRHQQLSHQVEASLGSVQKMEDYDGDDSFRKSALTMLEGYDKL
                     LKNEYTEAEKILAQPDSMYSDGDEARLEILYKQIDKKTEELMDNFKRAQDDFAKKYKL
                     NLKADTSKTNG"
     gene            complement(7678..9120)
                     /locus_tag="PK281_03805"
     CDS             complement(7678..9120)
                     /locus_tag="PK281_03805"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HOY48499.1"
                     /translation="MNWMIRFLLLAVFSLSTISGKAQSPYTTEQYSYRTEFDIPYGTS
                     INYAGNPVDVYLNLYKPVGDQNTHRPLIVLVHGGAWIAGNRNDNDIQLLAPRFVSRGY
                     VVASVSYRLGFHPNPNGGSNTATCTAVTQQGNCVYPADTNEVIRAIYRGMQDVKGAIR
                     FLKGRANLDSVCAENVYLAGVSAGGFNSLAAAFLDTPEEKPYSAGALSDAPGPSNPLA
                     YCHDYFNPVGASISRTRPDLGDIEGTIALNGHTSQVKGMANFYGGMLGNLFEQSAGEE
                     PLLYLFHQTTDVVVDCNTTPIMAPLSYNCLDPFGFLGCAHVWNMPWASGSCSIAQKAA
                     NVSNPLTVFNAIVQNGGPNCLQDPAGHSILGPAARVSEITEFFSDRIVAIEQSNCEEP
                     LLSKSAYLAEIRVFPNPADNFIQISSPKSIQSIKTRDSQGRMMQLDVDFSSKQVKLNT
                     SELAPGWYFSQILLDDGSSRFVRWMRVMGN"
     gene            complement(9236..10372)
                     /gene="hppD"
                     /locus_tag="PK281_03810"
     CDS             complement(9236..10372)
                     /gene="hppD"
                     /locus_tag="PK281_03810"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006989163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HOY48500.1"
                     /translation="MDNAQTLSPQATNAAAADFLPLLGTDYVEFYVGNAKQASYYYQS
                     AWGYELIAYAGPETGVKDRASYVLRQGKIRLVLTASLMPEGPIADHVKKHGDGVKVLA
                     LWVEDAAKALEETVKRGAKMHTPLEVKTDAFGEVRTASIFTYGETIHTFVERKNYTGV
                     FLPGYEKAEPQVPSKPVGLMFVDHCVGNVGWGEMNTWVKFYQEVMGFKIIVSFDDKDI
                     STEYSALMSKVVTNSNEFIKFPINEPAKGKKRSQVEEYLDFYQTPGVQHIAVATDNII
                     ETVAELRRRGVDFLEVPQTYYDELTLRVGDIAEDMDEIRRLNILVDRDDEGYLLQLFS
                     KPVEDRPTLFYEIIQRRGARAFGKGNFKALFESIEREQERRGTL"
     gene            complement(10392..11561)
                     /locus_tag="PK281_03815"
     CDS             complement(10392..11561)
                     /locus_tag="PK281_03815"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HOY48501.1"
                     /translation="MPFYHSLGKVPSKRHVVYNKPEGGIYYEQLFGTVGFDGMSTLMY
                     HLHRPTIVKSIGESIDVSPKVAIEKNMKSLRLVGFNVPPADDFLESRTALLVNSDVHL
                     GVAAPRKSMSTYFYKNADADEMLFIHKGTGRLRTFLGTLEFAYGDYLIIPRGMIYQIE
                     FDGEDNRLMFLESFSPIYTPKRYRNYFGQLLEHSPYCERDIRRPENLETHDEKGDFII
                     KVKKQGVLHHYVYGSHPFDVIGWDGYNYPYAFSIKNFEPITGRVHLPPPIHQTFETAG
                     FVVCSFVPRMYDYHPQAIPAPYNHSNIDSDEVLYYVDGDFMSRNDIAQGHLTLHPAGI
                     VHGPHPGAMERSIGKKDTQEYAVMVDTFKPLMVTEAALKINDDKYFLSWMEPDYN"
     gene            complement(11704..15555)
                     /locus_tag="PK281_03820"
     CDS             complement(11704..15555)
                     /locus_tag="PK281_03820"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOY48502.1"
                     /translation="MKTRILTTLLILLGGGYTAIGQVACGYGFSYSGNIPYTPLPSGN
                     QTLLLASGSADPATSTSVSPTDEDFFPDQPIGFAFNYNGTTYTQCGVATNGWIWFGNT
                     DPVKAAGIVIPFTQVLESEIGIEGIVSALNADLEGRWTAGLASIRTRTEGQAPNRTFT
                     IEWNNFKALDDAEGTGYCGENRNRFDFQIILEEQNNKISFAYNAAPYCWQGYNQLFQV
                     GLRGASRSDVHTRNIPAGNDSWAQSALGLSNSTAVIRSSSPVTIPAQNARFSFFPAQA
                     ATSTWLGISSDWNNPQNWNPAQVPNRCNSVIIPSGLSHYPELDGNQPVSCGNLLVSEG
                     AAVTLKSTFNSFLSCYGSLTNNGVITNNTESYLTLAGGANVTIGGNGHFIGSDLYITT
                     NSEYTLQNDLVIRNLSINDGSKLKLSDKILDVYAIQQRGTLDQGTGVLVIEGDASNVL
                     LTDSTFEENNGTTFFGNGEIWANAVDQVVPSVNYNHLWVRTTKDHVVLLGTDEDFSCK
                     NLLFYNPGEPGGQASTQHAITVRGDFRLGIDSLPGTELILNHTINRINGSGAFEMGKQ
                     DQLNVTHSSTAQQTVLSGFSTPIFKGTVSYTSSSEQTLVKGTYNNLNINGSGQRFIAG
                     QVNLRGILKLNSGTLHSNDSLSLKSDSLATALISGTGAGTLVGKVGAERFIEGTGTKE
                     VLLSSAFSDVAMEDYMRGIPTLGPDGISWQTQSAPAIWEYNESTGVSALSSGWQQGSS
                     NSVMHRMTGYKTQQTGGTTIMAKGTVNSGIQKLALSRSGSSGFAGYNLAGNPYPSPID
                     WNSVAATLPVSISRSIHTLPKSDRYTGQFATWLPLGSNEGLGINGATQYVGMQEAFFV
                     RAFNSDTLRLNNSHRADVTNVRSVSVPETIPFIRLSVNSNGKADETLVYYSQQANSNQ
                     ATDGKDAVKMEPGGQVSYWYSIKDSLHLAIQGRHITEHADSIPLDVVVRQGGTHQFRL
                     SEAIHFPMTAMIFLEDKVTHTIQNLRQQPEYTVVLEPGTIQGRFFLHYRPGVQVSALK
                     EGCLGGDGQITLNNPTSTAWDVEVFTSTDSLVGSHTALIGNWVIDQLKADEYRVHFSL
                     NGGTLETDEWVQVQAGSGITASFSASATEVKMEEEEVVFTSTTAGAESYFWNFGDGMM
                     VSGESEVNHTFTDAGVFPVVLTAGRNECSDTAVIQIYVINITGIEEAGTKEKASFTVF
                     PNPATTVAWLKPDLKENLKDAELAIIDISGKIAYQQKYSNINPGQLIEIPVSNLAKGN
                     YQVLIHAQGFRAVNSLVVGGK"
     gene            complement(15807..>16346)
                     /locus_tag="PK281_03825"
     CDS             complement(15807..>16346)
                     /locus_tag="PK281_03825"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY48503.1"
                     /translation="GSSPLVEVIQSQFPIANFAYSQGVGGYTISFNNNSQNGLSYEWA
                     FDSIGTSPLRDPSFTFPDSGPYDITLIISNPCGADTITKTIIVALVGMEDLGSGDEIN
                     VYPNPSDSDFHLVHTQAANTVSEIILRDLTGRLVFEKSFDPNQGLDLSIPGSTLPAGT
                     YVACVHFKSGYRYYRIAKK"
BASE COUNT         4697 a         3574 c         3149 g         4826 t
ORIGIN      
        1 cctttcaaaa ttacacttgg atatttccag gtaatagctg aaccggtttc aacttgagtc
       61 cacgaaattt tagctccaaa accggcacaa attccccgtt tggtaacgaa gttgaagatt
      121 cctccaacac catttttatc gcccggatac cagttttgaa cggtactgta tttcacttcg
      181 cctcgttctt cggctataat ttcaactact gcagcatgca actgattttc atcacgcatt
      241 ggtgcagtac agccttctag gtaactaacg taagcacctt cttcagctac aattaacgta
      301 cgttcaaact gacctgttcc agatgcattg attcggaaat aggtagaaag ttccatcgga
      361 caacgaacgc ccttaggaat gtaacaaaac gaaccatcgg taaaaacagc tgaattgagc
      421 gctgcgtaat agttgtcggt atacggaact acagaaccca tgtatttttt aatgagttcg
      481 ggatgatcat gcacggcttc gctaaacgaa cagaagataa ttcctaattc acccaacttt
      541 tctttgaatg tagtttttac cgaaactgaa tcgataactg catccaccgc aacacccgtt
      601 aatcgttttt gctctaacaa ggaaatacca agcttctcga atgtttttct caactcagga
      661 tctacctcat cgagactatt cagttgcttc gttttcttag gagcagcata gtaaatgatg
      721 tcttgaaaat cgatttttgg gtagttcacg tgtgcccaaa caggttcttc aagtgtaagc
      781 caataacgga aagcctttaa gcgatatgca agcatccatt ccggctcttt tttcttagca
      841 gagataaatc gaataatatc ttcattcagc cctttgggag cagaatccat ctcgatatcg
      901 gtggtaaatc cccacttata atcggaattg atatgcgatt ctaatatttc gtttccctca
      961 gcagccatga atctttattt taaacggcaa aactttcccc gcatccacat gtacgtgttg
     1021 cattcggatt attaaacgaa aatccttttc cattcaatcc tcccgaatat tccaacaccg
     1081 ttccgaccaa atacagaaag ctctttttat ccaccaggat ttttactcct ttgtcctcga
     1141 ataattggtc tccatcacga cgttccgcat caaaatccat tttataggat aaacccgaac
     1201 atcctccacc ttcaacacca acgcgcaagt aactatcagc actgtggtta ctttctgcca
     1261 tcagactaag tagtttttct tttgcagtat cagaaacaga aatcatacgt tacaaccatt
     1321 tagcttataa acacaagttc gaatagtatg tttcaaataa gccattttta tttagactta
     1381 ttctaaacag tctgcaaaga tatgctatga cgaggattta ttcaaccttc caatcgaatt
     1441 tgatttagga gacttgaatt atattaccaa tctaagtgtt gttagggacg tttaagctat
     1501 ttgagaatcc ttatcaactt atatatttgc caatatggga acggaaatga tgcctcagta
     1561 cgaagaaacg gaggaaaaga ttgtcaatca tcttgaggag tggattttag caacaaatcg
     1621 atctccaatt aataaaagcg ttggaggatt tttacgttcg gcttcacctg ctttaaacat
     1681 attagctaaa actcgagtag ccgcttttgt ctacgattac gagaatggta gctattctta
     1741 tttcaacgat tattttccca ttctactcaa ctcaactcgt cgacatatag agaaagttgg
     1801 aattcgggta atggaggaac gcgttcatcc cgaggatttt atcaagtgtc ttaaccttac
     1861 tcgacgaacg ttagaagagt tttctaaaat gaaagaagat gagaaagaca gcactcaact
     1921 tcgattgttc ttccgagtga agcgtaaaag cggtgattat gtgtgggtat tgcaaagtaa
     1981 tcgtcaggtg cgttggagta aagatgcacc agctctagat ctagcctata tgatggaatt
     2041 gttcgattcg cacaatccta tgaaggtaat ggctgttttg caaaccagtc gccgagtaat
     2101 ggaattttta cccgatggtg aaacggaatt actaagtcan nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnt ggctttcgga tatttgacgg ttgatggcgt taaatgcatc
     2281 aatctcttca gcaatcttgt cccgatctcg atcagctgca aaaggagtaa ctccccaatc
     2341 gggaatggaa acaaccacca catgattctt tattccacca gcaaattcaa tcgcccgatt
     2401 taataaagcg gtaaactctt gaacataaac ttcctgcgaa ttgccacgat attgattgtt
     2461 cactccaatc aacagtgaaa ccaagccata atcatttccg ggcttacggt cgtcaatggc
     2521 tttggaaagt tcatcggtag tccaacccgt tgtggcaata atttcgggtt gattcagatt
     2581 tactccagca tcatgcatca atctgctaag ctgaaccggc cagcgatctt gttctgcaac
     2641 tgattctcca atggtgtacg aatcgcctaa ggctaaaaaa cgaatgggtt cttgtgccat
     2701 catagtggta acgacaaaac taacaagtgt aaaaaggagc aagcgcatca aagctctttt
     2761 cgcattcggg ctaccggaat attgagttgt tcgcgatatt tcgctaccgt acgacgagcg
     2821 atgttgtatc ctttttcacg aagcactttc gccaatttat catcggttac tggcttgctc
     2881 ttatcttcgg ctgccacaca ttcttccaaa atctttttca cctctcgggt agaaacttct
     2941 tccccggtat cggtcgataa ggattcagag aaaaacgact tcaacaaaaa agttccaaac
     3001 gaagtctgaa tgtatttgct attagcaaca cgcgaaatag tggagatatc gaggaatact
     3061 ttctctgcaa tatccttcaa aatcatcggt ctcaacttcg tctcatcacc cgttaagaaa
     3121 tactcatact ggtaatcgag aatcgcttgc atggttagca tcaaggtttg ttgacgctgt
     3181 ttgatggcat cgataaacca acgagccgaa tcgatttttt gcttcacaaa caaaaccgca
     3241 tcgcgctgct ctttatcaga aaccttcttg gttttgctat aatgcaccaa catttcctga
     3301 tacgtacgac tgatgcgcaa atcgggggca ttgcgcgaat tcaactgcag ttcgagcact
     3361 ccttcattgt tattcaacaa gaaatcagga atgatgtgtt gtgtgctttt aggtgaatcg
     3421 ctctgagaat ttcccggacg aggattcaac ttcatgattt ctttgatggc agctttcaac
     3481 tcctcatccg taagttcgag tttcttggca atcttatcgt aatgcttgcg cgaaatctca
     3541 tcaatacact cacgcaaaat aacagcagcc gttttcatct caacggtttg gtgctcttta
     3601 cgacgaagtt gaatcaacaa acactcttgt aaatcgcgag ctccaattcc tggaggatca
     3661 agttcttgta cttctctcaa caaacgaagc acatcttctt cgctcgttaa tatgttttgt
     3721 gaaaaggcta aatcatctac aatcgcactt atatcacgac gtaaatagcc atcctcatca
     3781 atatttccaa tcaaatattc agcaatagga aattcttctt cactcacatt tcgaagcccc
     3841 aactgactta acaactgctc ctgaaaacct tgtccgcccg caaaaggaat ctccttacgc
     3901 tcatcatccg gactataatt attcgctgct agcttataag caggagtttc atcatcatcg
     3961 atataggcac ctaaatcaaa atcttcccga ttctgctccg aatgcgcttc tacatcacca
     4021 tcatcggtac tcgaaaacgg atcttcggat gtttcttctt tggtatcgtc ggcttcggca
     4081 ccttcttcta atgccggatt gatttcaagc tcttccttaa tgcgctgttc caatgaaaca
     4141 gttggcacct gcagcaattt catcaactga atctgctgag gagaaagctt ctgaagtagt
     4201 ttctgctgta atccttgctt aagcatcgtg ctgcaatgtt agtgaaaaac aagtttcgtc
     4261 aatctgcacc ggtcaagttc attatcttta cctcatcctg attggaaaac gcaaaaagtg
     4321 ctggtctgtt tcgttttttt ctttgaagaa cgattctttt ccgcatttgg cgaggatttc
     4381 aattttaatg cttgaatatc aaggacaaaa atttacaaat cgaatagggg ttgaaccttt
     4441 cccgaggtgt cttccgttaa aatacatgtt atgaaacccg ggaaaaacca atttttcaag
     4501 cctttaacat ctctattcgg aatctctttt ctgtttttgg ctgggttgtg ttttatcgca
     4561 aatccttcat ttgccgctaa ttcaacatta acacaacatc tcaagtatac cgtatatgtg
     4621 agcggcagca agcgtggcac tttacaagcc gatattaatc gcaagcccga taaatcgttt
     4681 gatatccgtc tcgaaaccaa acttcatttc ccatttaccg atgtagtttc actcattcag
     4741 gtgcactaca cagctcatgg tttggaaagc tcttcctcac tcaaacacat caacgacgat
     4801 ttaaaagaga aaatcactgt aaagcgcgtc gatggtggat atgcacttac tgatgtggat
     4861 ggaaaaaaca cccaacttct aggaaaaata ccctttagcg tgggccgatt ataccacgat
     4921 gaacctgtcg gaatgaaaga attattctcc gaacgactcg gcacatttgt tcccatccga
     4981 tcactcaaag caggcgaata cgagcttttg caacccgatg ggaaaaagaa tgtgttcgtt
     5041 taccgatacg gcatctgcac cgaaatgcgc accgaaatga tggcttgcaa tgttcggttt
     5101 gtgctaaccg attaaaattc caggtttttc ggtgtacgcg gaaatggtat cacatcgcgg
     5161 atatttccca ttccggtaac aaacaatacc aaacgttcga aacctaatcc gaatccactg
     5221 tgcggaaccg ttccatactg acgggtttct aaataccacc acaaactttc gcggtgaatt
     5281 ttcatctcat caatgcggcg actaagtttt tcgtaatctt cctcgcgctg tgaacctcca
     5341 atgatttcac caattccagg gaaaagtaca tccatcgcgc gaaccgtttt tccatcttca
     5401 tcttgcttca tgtaaaaagc tttgatgtct tttggataat tagttagaat aacgggtttc
     5461 ttaaaatgct tttctaccaa ataacgctcg tgctcgcttt gtaagtcaac tccccattta
     5521 acgggatatt caaacttttt accgctgcgt tctaaaatgc tgatggcttc ggtatacgtc
     5581 aaacgttcga atggctgctc agcaacaaaa ttcaattttt ccagaagcgc catcggattg
     5641 cgatcttctt tcttcatctg gctttcttcc tcaatcaggc gtttttctaa gaatgccaaa
     5701 tcatcggcac aatgctccat cgcataacga atgaggtatt tgaggaaatc ttccgccaaa
     5761 tccatattgt cttccaactc gaaaaatgcc atttctggtt ctatcatcca gaactcagct
     5821 aagtgacgag tggtgttaga attttcggca cggaatgtcg gtccaaacgt ataaacacgc
     5881 gaaagtgcca tggctgccag ttccgattct aattgtccgg aaacggtgag gtttgcatct
     5941 ttcccaaaga aatcttgagt agaatcaatg cttccatctt cgttacgtgg cggatttgca
     6001 gcgtctaatg tggtaacacg aaacatttca cctgcacctt ctgcatccga acctgtgata
     6061 ataggagcat ggatgtagta aaaatggttg tcgttgaaat acttgtgtat cgcaaatgct
     6121 aaagcgtgac gaatgcgcaa aattgctcca aacgtattgg tacgtgggcg taaatgggca
     6181 atctcgcgta gaaattcgag tgtgtgtttt ttcttttgaa gagggaactt ttcagcatct
     6241 gcttcgccta atacttcaat cttagttgca gatagatcga ctgcttgtcc ggcaccttgt
     6301 gaggcaacta aattgcccgt tacacgcagg ctgcagcccg tttgaacttt cttaagcagc
     6361 tcttcatcga acttagaaag atccgcaaca atttgaagat tattgattgt tgaaccatcg
     6421 cttagtgcaa taaaggcaat gtctttatta ccacgtttgg ttcgtaccca gccggataca
     6481 gtaatttctt ggttttcggg ttgggtttta agccaatcgg caattcgttt ctgatgaagc
     6541 gcactcatag gttaaatcta ttgaaggcgc aaaaatagtt cttccaatgg aattcaaggc
     6601 cattaacctt gaagcagttt caaatgtgct gtgtgatgct tgccatgcca gctgtacaag
     6661 gcacataaat acccaatgga acgtgtgtat tgatactgtg gatgggtaaa catgcggtga
     6721 aaatcaagct cagtcattcg gtcgaatatg taaaccaaat gtgcatgcgt gttctcaatc
     6781 aaatccaaag aaatcgaaat gggcatctcg taatccgata agcgagccca tttttcctca
     6841 ttataaggtt tgatggttgg attatcttcg gttaaagcca gcttcactcg aacgaggcaa
     6901 ttgagatgcg aatcagccac atgatggatg acttgtttgc ccgtccaacc ttctggacga
     6961 taaggcttgg ctaattgttc ttcacttaag ctattagcca cagcacgaat ttcttgaggc
     7021 aaactgcgga tgttttcaat ccaagatttt atatccgcca tgggataggt ttccggtacc
     7081 accaatttgc caatcgggaa tcgaagatgt tctaaattat ccattggttt tgctcgtatc
     7141 tgcctttaaa ttaagcttgt atttcttagc aaagtcgtcc tgcgcccgct tgaaattatc
     7201 catcaactcc tcagttttct tgtcgatttg cttatacaga atttccaggc gagcttcatc
     7261 accatctgaa tacatgctat cgggctgtgc taggattttc tctgcttcgg tatattcatt
     7321 tttaagcagt ttatcatatc cttctaacat cgtaagggca gatttacgaa aggaatcgtc
     7381 accatcgtaa tcttccattt tttgcacact gcccaaggat gcttctacct gatgactcaa
     7441 ttgctgatga cgcatacgca tttctgcttc cacgtaggag ttaaatgcat cctgcaactc
     7501 atcggctttt tcaatcaccc gaatttgctg aatcactaac gaatcgttgt acacttcagg
     7561 ttttacggat gggccgccac atgccattaa agtcattccg gcgaacaggg atagatagat
     7621 tcgggacttc atatttacaa agaacgcaaa tcgtggcaat ccgaaaaaga aaatcgatta
     7681 attacccata accctcatcc aacgtacaaa ccgactcgat ccatcatcta acaaaatctg
     7741 cgaaaaatac catcccgggg ccaattcact cgtgtttaat ttgacttgtt tggaggaaaa
     7801 atctacatcc agttgcatca tcctaccttg actatcgcgg gttttaatac tttgaatgga
     7861 tttgggtgag ctaatttgga tgaaattatc agcaggatta gggaaaacac gaatctcagc
     7921 taaataggcc gatttactca aaagtggctc ttcgcaatta ctctgctcaa ttgcaacaat
     7981 ccgatcagag aagaattcgg taatctcact aacacgtgct gccggcccaa gaatagaatg
     8041 acctgccgga tcttgcaaac agtttggtcc gccattttga acaattgcat taaatacagt
     8101 taatggatta ctaacattag cagccttttg agctatagaa caagaacccg aagcccaagg
     8161 catattccaa acgtgagcac atcctaaaaa gccaaaagga tcaagacaat tataacttaa
     8221 gggagccatg ataggcgtgg tgttgcagtc gacaaccaca tcagtcgttt gatgaaacaa
     8281 atacaacaaa ggttcttctc ctgcactttg ctcaaacaga tttcccagca tgccaccgta
     8341 aaaattggcc atgcctttta cttgggaagt atgtccattg agtgcaattg ttccttcaat
     8401 atcacccaaa tcgggacggg tgcgacttat agaagctcca acaggattga aataatcgtg
     8461 gcaataagca agcggattcg atggacccgg agcatcactc aaggctccag cagaataggg
     8521 tttttcttca ggggtatcta aaaatgcagc agcaagtgaa ttaaatccac cagcactcac
     8581 accagcgaga tagacgtttt cggcacaaac ggaatccaag ttagcacgac cttttaggaa
     8641 tcgaatggct cctttcacat cctgcattcc gcgatagata gcacgaatga cttcattggt
     8701 atctgccgga tagacgcaat tcccttgttg agtaacagct gtgcaagttg cagtattgct
     8761 tccaccattg ggattggggt gaaatcctaa gcgataactt accgatgcta ctacataacc
     8821 ccgacttaca aaacgaggtg ctaataattg aatgtcgtta tcgttgcggt tgccggcaat
     8881 ccaagcacca ccgtggacta gcacaattag tggtcgatgg gtattttgat cgcctaccgg
     8941 tttatataag ttgaggtata catcaaccgg atttccagca tagttgatgg aggttccgta
     9001 gggaatatcg aattcggttc ggtagctgta ttgctcagtt gtgtaaggcg attgagcctt
     9061 tcctgaaatt gtagaaagag aaaaaacagc gagcagtaaa aaacgaatca tccagttcat
     9121 gaaagcaatt tacagctttt cataaacatc acttgaaagt ttgattgggt ggataaaaag
     9181 aaatcccgtc ttgcaaggag gcgcaaaacg ggatttcgaa ctgaatcaca aaaacttaaa
     9241 gcgtaccgcg acgttcttgt tcgcgttcga tagattcgaa aagggctttg aagtttcctt
     9301 ttccaaatgc acgagcaccg cgacgttgaa tgatttcata gaataaagtc ggacgatctt
     9361 ctactggttt agagaaaagt tgaagtagat aaccttcatc gtcacggtca actaaaatgt
     9421 ttaggcgacg aatttcatcc atgtcctcag cgatgtcacc tacacgtaag gtaagttcat
     9481 cgtaatacgt ttgaggaact tcaaggaaat caacaccacg acggcgtaat tcggcaacgg
     9541 tttcgatgat gttgtcggtt gcaacagcaa tgtgttgaac tcccggtgtt tggtaaaaat
     9601 ctaggtattc ttctacctga gatcttttct ttcctttagc aggttcgttg atcgggaatt
     9661 tgatgaactc attgctattg gttaccactt tcgacatcaa agcagaatat tcggtagaga
     9721 tatctttatc atcaaacgaa acgatgattt taaagcccat cacttcttgg taaaatttca
     9781 cccaagtatt catttcaccc catccaacat ttccaacgca atggtcaacg aacattaaac
     9841 caactggttt tgaaggcact tgaggttcag ctttttcgta accgggtaag aaaacaccgg
     9901 tgtagttttt tctttctaca aacgtgtgga ttgtttcacc atacgtaaaa atactagccg
     9961 tgcgaacttc accgaatgca tcggttttaa cttcaagtgg agtgtgcatt ttagcaccgc
    10021 gtttaacggt ttcttctaat gctttagctg catcttctac ccataaagcc aaaactttca
    10081 ccccatcgcc atgttttttc acatgatccg caataggacc ttctggcatt aatgacgcag
    10141 ttaaaaccaa acgaatttta ccttgacgta atacataaga agcgcggtct ttcacacctg
    10201 tttctggacc agcgtaagcg atgagttcat aaccccatgc gctttgatag taataggaag
    10261 cctgtttggc attacctaca taaaattcaa cataatcggt tcccaataaa ggaaggaaat
    10321 cggcagccgc agcgtttgtt gcttgcggag atagcgtttg tgcgttatcc atgatgattt
    10381 ggttttattg cttagttgta atcaggttcc atccaagaaa ggaagtactt atcgtcattt
    10441 atttttagag cagcttcagt taccattaaa ggcttgaatg tatcaaccat aacggcatat
    10501 tcctgcgtgt ctttttttcc aatgcttcgt tccattgctc caggatgcgg accatgaaca
    10561 attccagcag ggtgaagggt aagatgccct tgagcgatgt cgtttcgact catgaaatca
    10621 ccatctacat aatataacac ctcatcagag tcgatattgc tgtgattgta tggggccgga
    10681 atcgcttgtg gatgataatc gtacatgcgt ggtacaaatg aacatacaac aaaaccagcc
    10741 gtttcgaatg tttggtgaat aggtggtgga agatgtacgc gaccagttat cggttcaaaa
    10801 ttcttaattg aaaacgcata cggataatta tatccatccc aaccaattac atcaaacgga
    10861 tgagagccat atacgtaatg atgcaatact ccttgtttct tcaccttaat gatgaaatct
    10921 cctttttcat cgtgagtttc gaggttttct gggcgacgaa tatcgcgttc gcagtaagga
    10981 gaatgctcta ataattgtcc gaagtaattc cggtaacgtt ttggagtgta aataggggag
    11041 aacgattcta aaaacatcaa tcggttatct tctccgtcaa attcgatttg ataaatcatc
    11101 ccgcgaggaa taatcaaata atcaccataa gcgaattcaa gcgttccgag gaaagtacgt
    11161 aagcgacctg ttcctttgtg aatgaaaagc atctcatcgg catcggcatt cttgtaaaaa
    11221 tacgtgctca tcgatttacg tggcgccgct actcctaaat gcacatccga attaactaat
    11281 aaggcagttc tgctttctaa aaaatcatcc gcaggtggta cattaaaacc aaccaaacgt
    11341 aatgacttca tgtttttttc aatcgccacc ttaggggaaa catcaataga ttcaccaata
    11401 gatttaacga ttgtagggcg atgtaaatga tacatcaatg tggacatacc atcgaaacca
    11461 acggtgccga atagctgctc ataatagatt ccaccttcgg gtttattata tacgacgtgg
    11521 cgtttagaag ggactttacc cagagaatga taaaacggca tctgtgatga tttgtattgt
    11581 gtatcacaaa cgtaaaggat ttcttttttc caagcaagca caaaatcaca acggaatttt
    11641 ggctttacaa ctcgattcgg aataaaattc tgcaattatc aagtattgca aaatacaatg
    11701 ctgttatttg ccgcctacta ctaaagaatt tacagctcta aacccttggg catgaattaa
    11761 tacttggtaa tttcctttgg ctaagttgct taccggaatt tctatcaatt gtccgggatt
    11821 aatattcgaa tatttttgct gataggcgat tttacctgaa atgtcaataa ttgctaattc
    11881 ggcatctttc aaattctctt tcaaatcggg tttgagccaa gctacagttg ttgctggatt
    11941 cgggaataca gtaaaactag ctttctcttt ggtgccggct tcttcgattc ctgtaatatt
    12001 gatcacataa atctgaatca ctgctgtatc actacactcg tttcgaccag cagttaacac
    12061 aaccggaaaa actcctgcat cggtaaacgt atgattcact tccgattcac ccgaaaccat
    12121 catcccatca ccgaaattcc agaaataaga ttcagctccc gctgttgtac ttgtgaaaac
    12181 aacttcttct tcctccattt tcacttcagt agcagaagcg gaaaatgaag cagtaattcc
    12241 acttccagcc tgaacttgaa cccattcgtc ggtttctaat gttcctccat tcaaactaaa
    12301 atgaacgcgg tattcatcag ccttaagctg atctattacc caattaccaa ttaatgccgt
    12361 gtgacttcct actaaactat ctgtcgaagt aaaaacctct acatcccaag ctgtactagt
    12421 aggattattc aatgttattt gtccatcacc tcctaaacat ccttctttca aagcactaac
    12481 ctgaactcct ggacgataat gcaaaaagaa tcgtccttga atggttcccg gttctaaaac
    12541 taccgtgtat tcgggttgtt gacgaagatt ttgaatggta tgtgtgactt tatcttccaa
    12601 gaaaatcatg gctgtcatgg ggaaatgaat agcctccgac aaacgaaatt gatgtgttcc
    12661 accttgtcga actacgacgt ctaaaggaat agaatcggca tgttcggtga tgtggcgacc
    12721 ttggattgct aagtgcaacg aatcttttat tgaataccaa tagctcactt gtccgcccgg
    12781 ttccattttc acggcatctt ttccgtctgt tgcttgattc gaattggctt gttgcgaata
    12841 gtaaaccagc gtttcgtctg ccttcccatt tgaattaaca ctcaatcgaa tgaacggaat
    12901 cgtttccgga acgcttacag atcgaacatt tgttacatcc gcacgatgtg aattgtttaa
    12961 acgtaaggtg tctgaattaa aagcacgtac gaaaaatgct tcttgcattc ctacatattg
    13021 agttgctcca ttaattccta atccttcatt tgaacccaaa ggaagccaag tggcaaattg
    13081 tccggtatag cgatccgact taggtaaagt atgaatgcta cgactaattg aaacaggcaa
    13141 agttgccgca actgaattcc aatcgatggg cgatggatat gggtttccgg caagattata
    13201 tccagcaaaa ccgcttgaac cacttctact caaagcaagt ttttgaatgc ctgaattaac
    13261 tgttccttta gccatgatgg ttgtgccacc agtttgttgt gttttataac cggtcattcg
    13321 atgcatcacc gaattgcttg aaccttgttg ccatcccgaa ctaagtgcac taacgcctgt
    13381 actttcgtta tactcccaaa ttgctggagc cgattgagtt tgccagctaa ttccatccgg
    13441 tccaagtgtt ggaattcctc tcatataatc ttccatcgct acatccgaaa atgcactact
    13501 caagaggact tctttcgttc ctgttccttc aatgaatcgt tctgcaccta cttttcctac
    13561 taatgttccc gctcctgttc ctgaaattaa tgctgttgct aaactatctg actttaaact
    13621 caagctatca ttcgaatgga gcgtgccaga attgagtttt aagattccgc gcaaattcac
    13681 ctgtccggca atgaaacgtt gtccgcttcc attgatgttc aagttgttat aagttccttt
    13741 tactaatgtt tgttccgaac tgcttgtgta agaaacggtt cctttgaaaa ttggagtaga
    13801 gaatccggac aaaacagttt gttgagcagt tgaggaatgt gttacgttaa gttgatcttg
    13861 tttgcccatc tcgaatgcgc cacttccgtt gatacggttg atggtgtgat tgagaattaa
    13921 ctcagtgccc ggtaaactat caatccctaa acgaaaatct cctctaacag taatagcatg
    13981 ttgcgtactt gcttgaccac ccggctctcc tggattatag aaaagtagat tcttacacga
    14041 aaaatcctca tccgttccta ataaaacaac gtggtcttta gtggttctaa cccacaggtg
    14101 attatagtta accgaaggaa ctacttgatc tactgcattg gcccaaattt ctccatttcc
    14161 gaaaaaggtg gttccgttgt tttcttcaaa ggttgaatcg gttagtaaca cattagatgc
    14221 atctccttca attaccaata ctccagtacc ttgatctaaa gttcctcttt gttgaatggc
    14281 ataaacatcc aaaatcttat cgctaagttt caacttggat ccatcattta tagatagatt
    14341 ccgaataact agatcgtttt gaagcgtgta ttccgaatta gttgtgatat acaaatcgct
    14401 tccaataaaa tgtccgtttc ctccaatagt cacatttgca ccacctgcta atgttaggta
    14461 tgactccgtg ttattggtga ttacaccatt gtttgtgagt gaaccataac agctcaaaaa
    14521 cgagttgaac gtacttttta acgttactgc agcaccctca gataccaaca gattcccgca
    14581 actaacaggc tgattgccat ctagttcggg atagtgactt agtccgctag gaatgataac
    14641 cgaattacat cgattgggga cttgtgcagg attccagttt tgtggattat tccaatcgga
    14701 gctgattcct aaccaagtgc ttgttgcagc ctgagcagga aagaaactaa atcgagcatt
    14761 ttgagcagga atagtaacgg gtgatgatga gcgaataaca gctgttgaat tcgataatcc
    14821 taatgccgat tgtgcccaac tgtcgtttcc tgccgggatg tttcgtgtgt gaacatcgct
    14881 tctactggct cctcttaaac cgacttgaaa caattgatta taaccttgcc agcagtaggg
    14941 tgcagcatta taggcaaacg aaattttatt gttttgttct tcgaggatga tttgaaagtc
    15001 gaaacgattc cggttttcac cacaatatcc ggttccttcc gcatcatcca aagctttgaa
    15061 attattccat tctattgtga aagttctgtt aggagcttgt ccttctgttc gagtgcgaat
    15121 acttgctaaa ccagcagtcc agcgtccttc caaatcggca tttaaagccg aaacaattcc
    15181 ttcgatacct atttcacttt caagtacttg ggtaaaagga atgacaatac ctgcagcttt
    15241 taccggatcg gtatttccaa accaaatcca accattcgta gctacaccac attgagtata
    15301 ggttgttcca ttataattga aagcaaaacc tataggttga tcagggaaga aatcttcgtc
    15361 ggttggagaa acagaagtac ttgttgccgg atcggccgag ccacttgcta aaagaagagt
    15421 ttgatttccc gaaggaagtg gcgtataagg aatattaccc gaataactaa agccatatcc
    15481 acacgctact tgtccaatag ctgtatagcc accgccaagt aaaatcaata aggtcgtaag
    15541 gatacgtgtt ttcaagtcgg tgtgctctat gataattagc tttcaccttt atacagccct
    15601 taagcatgaa aggtttcaga acgagagtac accaaaaaga aaaaggagcc gaagctcctt
    15661 tttctttcac aaaccaccaa ttgaattctg taaatctacc aaaaacagat cgattggcat
    15721 tggttatcgg ggtcaataac cactacaaaa gtataaaata gaattgataa aaaaacaccc
    15781 atttttcttt ttgaataagt tggggtttat ttttttgcaa ttcggtagta acgatatcct
    15841 gatttgaaat ggacacatgc tacataagta ccagctggca atgtgcttcc aggaatactt
    15901 aagtcaagac cttggttagg atcgaaactt ttttcaaaaa cgagtcttcc cgttaaatca
    15961 cgtaagataa tttctgatac cgtatttgcc gcttgtgtat gaacaaggtg aaagtcggaa
    16021 tcggaaggat tcgggtaaac gtttatttca tcccctgaac ctaaatcttc catacctacc
    16081 aatgctacaa tgatagtttt agttatcgta tctgctccac aaggatttga aatgattaat
    16141 gtgatatcat atggaccact atctgggaaa gtaaacgaag gatcacgaag tggagaagtt
    16201 ccaatcgaat cgaaagccca ttcgtaactt aagccatttt gcgaattgtt attgaagctt
    16261 atggtatatc ctccaacacc ttgtgaataa gcaaagttcg cgattgggaa ttgggattga
    16321 attacttcaa ccaaaggtga acttcc
//
LOCUS       DAOOQW010000036        16308 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_4335_length_16308_cov_5.871408, whole genome shotgun sequence.
ACCESSION   DAOOQW010000036 DAOOQW010000000
VERSION     DAOOQW010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 16308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16308
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4335_length_16308_cov_5.871408"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(108..1802)
                     /locus_tag="PK281_03830"
     CDS             complement(108..1802)
                     /locus_tag="PK281_03830"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOY48504.1"
                     /translation="MMKQLVVILLISLNLSAFAQTNGTFNRKYWVQLTDKVGTPYSLS
                     NPEAFLSTRAIDRRAKQGIGFKENDLPLNPAYVAQIQAAGARVINQSKWFNAVTIELT
                     DSTLLPIIEALPFVARTNKVARVKQKSPADQFIADLMKMQEQYEADAAKKLLLAQGAD
                     AFYGNGDQQIKMLNGDKLHQMGFQGKGIHIAVLDAGFYHVDQIVFFDSLRSSGRLLGT
                     RDFVQGGTSVFEDNSHGLSVLSTMAGNIPGVFVGTAPKASYWLLRTEDADSENLIEED
                     NWVRGAEFADSVGVDVINSSLGYTVFDDSTASHTYSQLDGKTARITIGADIAASKGML
                     IVNSAGNSGADPWKYIGFPADGDSVLSIGAVDVNRNYASFSSQGPTPDGRIKPNVTAM
                     GQQPYVITSGGMPGKSNGTSFSSPILAGMSACLWQAHPEASAMEIFKAIEQSADQYDA
                     PDAKKGFGIPDFLKAHAILSQLALDRAVQDSVVNVYPNPFIEGVTVEFYSTSEQDIQV
                     KIFKLSGKQIAEEKIHVYPYVNNLIQLDKIRKLNSGQYVVSVITPTRTFSRQVIKR"
     gene            complement(1799..2482)
                     /locus_tag="PK281_03835"
     CDS             complement(1799..2482)
                     /locus_tag="PK281_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48505.1"
                     /translation="MNKRYILFSLACLSLLISSCKKDSVIAPDPSYRNYFSDKVGSYI
                     IYQCDSIIYDDFNQTVDTNSFLIKEYFESTFIDNSGRSAIRIERWKKPADTLSWFLKD
                     VWQCVKTDAQVEKVEEDVRFIKMVFPVRESIEWNGNALNSLTAQTLTFSEVHKPFTTG
                     TLDFDSTITVVNTDPENLVSELRTTEVFAANVGMVFKKYVDVKYVVPTSNIKSGVVFT
                     MKAIEIGVQ"
     gene            complement(2490..3584)
                     /gene="mnmA"
                     /locus_tag="PK281_03840"
     CDS             complement(2490..3584)
                     /gene="mnmA"
                     /locus_tag="PK281_03840"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017842968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOY48506.1"
                     /translation="MSRHGKILVAMSGGVDSSVAAMMLHEEGYEVIGITMKTWDYASS
                     GGTKKETGCCSLDSINDARSLAVRFGFPHFILDIRGEFGNYIINNFVDEYIAGRTPNP
                     CVLCNTHIKWEALLKRADMLDCEFIATGHYAQHRFQDNGRYVISRGLDLNKDQSYVLW
                     GLSQESLARTRFPLGGFRKSEIRQMAADSGFVDLANKSESYEICFVPDNDYRGFLKRQ
                     VPGLEEQVDGGDFVLSDGTVVGKHRGYPFYTVGQRKGLEIALGKPVFVLDINPETNTV
                     ILGEKEELVKHTMNVRDINLMKYAELPEDFQALTKIRYKHEGTSSILNMRPDGLLQVD
                     FLDAVSGVAPGQSAVFYEGNDLVGGGFIKK"
     gene            complement(3581..4330)
                     /locus_tag="PK281_03845"
     CDS             complement(3581..4330)
                     /locus_tag="PK281_03845"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48507.1"
                     /translation="MNTLKILSLFLFVLVFSSSASSQATLNKTDSKGKKQGFWRKNYP
                     DGTPRYEGNFKDDNPTGVFRYYHETGELKMVCFYSDKGRRSHAKGFDAKGNMISDGNY
                     VDQKKDSVWTYYNEQKQVLSRETYIKGMKTGTWYVYYPEGPVSEEVTYINDIKQGPWI
                     QYFEDSTKRMTANYKDGKLHGKMIYYHANGKMKLSGNYINDLRQGTWYHGDENGNIKD
                     TELFKDGVSDKPNIQKFEKLEKDESEILPGQ"
     gene            complement(4369..6012)
                     /locus_tag="PK281_03850"
     CDS             complement(4369..6012)
                     /locus_tag="PK281_03850"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOY48508.1"
                     /translation="MSIPLQVTAGFGEIRPNHFHSGIDFSTQGKNQAVLAAAEGYVSR
                     IKVSQSGYGNAIYINHPNGLTTVYGHLAEFNDTINQYVSTIQQQSREYELDVLPDPLD
                     FPVSRQQFIGWSGNSGSSTAHHLHFEIRDQGFENVLNPLLFGYAEKDDFAPEINAVSI
                     FPMDDYGRVNNLKTNLNLPLVVNKKTKKKGISPKIKMPIVSGWVGFGFQGGDVIGKLR
                     SKTGIYKIQLLVDSTEVFQARFDEFSFDETRSVNAYQDYPERLKTRRKIQRCVVPDNP
                     MIGIYKKQLDRGYYYFSEDSIYTITYVLSDLNNNQTRFSIRVRGKKPDWEITPTQVSS
                     SYIPVLPGLAQTISLPDFQAEFQEETLFDTARIKLISHPSSFSPWIELGSKYIPVNQA
                     VRLRFKPSNVPDSLQAKLVVAQKNGTNLRGLKTNRAEDWLEAETFEFGDFQVVADTVS
                     PSVKVIPAFIRKKRKKIPLKQTASGRIRVQISDKLSGISSMQAYLDGNWILLEPGLSD
                     GEWTYKFPDELSEGDHPFRIEARDGVGNTRVFELLLPKP"
     gene            6208..6504
                     /locus_tag="PK281_03855"
     CDS             6208..6504
                     /locus_tag="PK281_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48509.1"
                     /translation="MTDLLGKSAVLEGKIKKLIHLHVAFKAENSALKSDNQRLTETIN
                     KQQEHIQQLEEGNKALKLAQALIQGSENEDPTVLKGKINEMIRDIDKCLALLNK"
     gene            6515..6808
                     /locus_tag="PK281_03860"
     CDS             6515..6808
                     /locus_tag="PK281_03860"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HOY48510.1"
                     /translation="MQDVSIEIQLANRKYPLKVKSAEANSVQKAATLINQKLSELETK
                     YGVKDIQDLFAMSALQITTQLIELESNNTGNQDQVFFRLEEMDQLITSILDEK"
     gene            6867..8408
                     /gene="rny"
                     /locus_tag="PK281_03865"
     CDS             6867..8408
                     /gene="rny"
                     /locus_tag="PK281_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HOY48511.1"
                     /translation="MEILIYVIVAIVGLGGGYAFATTVLRKNIEQKSAQLVKEAEAEA
                     EVIKKDKMLQAKEKFLQLKTEHEKSIQIRNQEVQQSENRAKQKESMLSQKLEETKRKQ
                     SELDVLRENLTQQLDVITKKQSELDKQHRRQVEQLEIIAGLSADDAKNQMMEALKEEA
                     KTEAASHIKDIMEESRLTANKEAKRIVVQTIQRVATEHAVENSVSVFNIENDEVKGRI
                     IGREGRNIRAIEAATGVEIIVDDTPEAIILSCFDPVRREVARLALHQLVTDGRIHPAR
                     IEEVVAKTKRQIEEEIIETGKRTCIDLGIHGLHPELTRMVGRMKYRSSYGQNLLQHSR
                     EVANLCAIMAAELGLNVKLAKRAGLLHDIGKVPDDEPELPHAILGMKLAEKYKEKPEV
                     CNAIGAHHDEVEMTSLISPIIQVCDAISGARPGARREIVESYIKRLKDMEQLALSYPG
                     VSNTYAIQAGRELRVIVESERVTDKDAEMLSFNISQKIQNEMTYPGQVKVTVIRETRA
                     VAYAK"
     gene            8552..9841
                     /locus_tag="PK281_03870"
     CDS             8552..9841
                     /locus_tag="PK281_03870"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOY48512.1"
                     /translation="MTIYPFLILTVLLFSASFFLPKQTNLPTNLKLALVFFTLIALAG
                     IVHYSFVQGKSEAKFEIRSLILQVLTVSMLFQTFRFAGSELFFKKVYQLSILLFVGLC
                     LIGWFEFYTGIHIQGFHTEKLIQLPVGNITFAPVFIYDNNNTFLCYLFSLALIILLSR
                     VGKRHSFPQVTSIIFQLLFFSIVADSRFGKIISVVFFIFYIASFINIDFIKSNSKLIK
                     LSAFFLFCVIGVFAFNSIFLGPLWKNGRDYKLNSIQVISENNGKPVFSSPDSLIKVIG
                     KEKVLKAYEKYEMHGKLSSIFIRKNLLLNGIFLTKEHPLFGVGPGQYSWYTIKNNVPY
                     VTGTITSPHEGLTELVSQYGIPLTLLFFFVLLLTFLRFFKGRKFDLFKTAKWILCLAV
                     FWIVAAMPSAWLVLNCGWILIAIILLSPENVTKASEQ"
     gene            9831..11090
                     /locus_tag="PK281_03875"
     CDS             9831..11090
                     /locus_tag="PK281_03875"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOY48513.1"
                     /translation="MSSKSSRSFTQNFLILFTGNSIGQAIPFLLAPFISRIYKPEEIA
                     VQENFLAMASLIAIVAAGRFDLAILIPTDDKKAKSLFSLSFIITTIVSLLSLITLLFT
                     NQIAQFYNTPELSQYLVFLAPSIFLLSSLSIYNQWVLRNKKYPLLTSMRVLQSLVQNS
                     GYVVLGYLGWGISGLFWAWIIGLFLPIAWMTIQERGIFKSLSNSRKELKDVAYEHRDF
                     PMVNSIHAFTDILATQFLLYWLITHNYGALALGLFSVMNRYLRAPLNLVGSAVGQLYF
                     REVGAKQGESSEQLKLFYRSIRIISLVIVPALVLVLFKGPWIFEIYLGENWREAGVLA
                     QIMAPAILFNFIASVVSTTPLIFHKQKPAYVIGLTGHILSLGTLFIGSLTTYNFHQIL
                     MAYSGVLSLNYIFLIFWYRSLIPKAAI"
     gene            11087..11989
                     /locus_tag="PK281_03880"
     CDS             11087..11989
                     /locus_tag="PK281_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48514.1"
                     /translation="MNVVVNGLPYFSRKLVADLQGFAPQHRFRFYDTYNSKIEQVKFA
                     WAIRKADVLISINGVSDRSGSMDLALKMNKKIWFNWVGTDVLLAVERFKNSSIFRDYI
                     DRAVHSTDAPWLQAELKTAQIEAKIVHSKWLDIQSNASDFPRLQVYSYVAANREDFYG
                     WNTIQQLALANPEIPFIIAGSDGTQLNSIPPNVQFKGWLTTQEMTELRNASPIFIRLA
                     EHDGFSLSVLEALAAGCEVIWKYPFENCHHLKDIEVDFSKVVNLVKERNFTPNKENIA
                     YIAKNFQKEVILNNFIQELEKIVG"
     gene            11979..13199
                     /locus_tag="PK281_03885"
     CDS             11979..13199
                     /locus_tag="PK281_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48515.1"
                     /translation="MSDNRIHIALVTAWFPPRKSVAVNRMLAFVNYISKERFKITVIT
                     LNEGNSPAHETNQTGDIYREQDEQIIPFLKNKQGENKLLHLSKVVWNVLLLKVLQYPH
                     KAWMKRVEKRLEFVHSKNPIDLIISSFSPIEAHLATYNFLQNHKSIAWVADMRDEMST
                     NGQINNQTRKLLQKAEIKINSRAQAITTVSSPILNDMRRIMTQVQEFAEIRNGFDNDL
                     KPDRNHNSVFTIIYAGTFYGPRKPHTFFEGLRIFLSTHTVEFKIQFLGTHHNFSIPKE
                     FENKCEFIPPVDNNKALEYISKADASLLILNEINRVGVYSGKIFDYISVQKPIISVLN
                     PNDVAADLIREQNAGYIADFNQPNEIARSIEFAWKIWNDKSYLPVDEAKVKTLHRKYQ
                     VKILEDLILKIVSK"
     gene            13196..14221
                     /locus_tag="PK281_03890"
     CDS             13196..14221
                     /locus_tag="PK281_03890"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOY48516.1"
                     /translation="MNVLQIGSDSIHLRHFCKAMSKLVGNLTLVTETPFSPEGTNKNL
                     VYPFRGLNLLGWIKNYHLLKKEIQSIKPDLIHIHQVNRLAWIVCRIAKSLRIPVITTA
                     WGSDVLIIPQKNSLYYSITKSVLDYSTYVTADSHEMIRAMQTISPADKKYVYLQYGID
                     PVKPQLKQRIIYSNRLHKELYRIDLIINLFSEFYKQHPDWKLIIGATGIQTDSLKELA
                     AKLLPSSAYEFIGWVDQETNANFYSLAQIYISLPNSDGTSVSLLEAISADCIPVVSDL
                     EVSREWITNQENGIIYHSSENPFEQALNVDSEKCFQINQELITKHALRATCMNKFEEL
                     YFKLKAI"
     gene            14218..15423
                     /locus_tag="PK281_03895"
     CDS             14218..15423
                     /locus_tag="PK281_03895"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOY48517.1"
                     /translation="MNWKEIIAQPLLILTLLLAAVLPLSQPLIPLVIVLLLAFYSWKG
                     HWKFSWDLVRSSPEILALLGFYVYNVLGLIGTENYSKGFIHLEIKLSLFLLPWLIFAR
                     SDLNLSKKKLINQTFIGACLIYCIWLLGRSTFIYITKGQNEFSYELFSNGFHPSYLAM
                     YLLLAIRLCYAEIQSIQTKLIIPYLLIIAVFVSCIVLLSSKINLFLLVFSFLYFFVEG
                     FIRAKRKTYWIYTAAAFIGIIAVSVFYNPSISERAERSFQVFSNLNTVDKSDTESNAA
                     RILIWQSAWTLMKEHPMGVGVGDVNIELENQYRLDGFTGIESKKLNAHNQFLQTGVAL
                     GFPGLISLLLLFILPIISRNKRQSGILMYFLVICFVNALVEGILETQKGVVFFGFFYC
                     LLLKEDERI"
     gene            15410..>16308
                     /locus_tag="PK281_03900"
     CDS             15410..>16308
                     /locus_tag="PK281_03900"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOY48518.1"
                     /translation="MSASKRVSVVIPCRNEEKYIAKCIESVLASNYPSDMLDVFVCDG
                     MSTDSTREIVKNFHENNRVTLLDNLQITTPFALNLGIEKSLADIIIILGAHAELDKDY
                     VKLCVETFEIDPEIGCVGGILDTISLDENSAAIALAMSSVFGVGNAHFRTGLKAGYVD
                     TVAFGAYRKEVFERVGLFDSALTRNQDDEFNFRLIQGGFRIYLNPNIRAKYYVRSTFS
                     KLYKQYKQYGYWKVYVNRKFKVVTTLRQLAPPLWVLFLIALPFAWLIHPILGTIHLIL
                     LLLYVLMSLLMSLRISRHVKEFGL"
BASE COUNT         5037 a         3127 c         3082 g         5062 t
ORIGIN      
        1 tcgtccatac cgtgagaaag aaagcggtgg cggatatcgc tcatcaggag gaggttcttc
       61 ttctggtggc ggatttaaag gcagaaagaa atactaatag cgtttgatta acgcttgata
      121 acctgacgac taaacgtgcg tgttggtgta ataaccgaca ccacgtattg tccggaattg
      181 agtttccgaa ttttatctaa ctgaatcagg ttattgacat acggatacac gtgaattttc
      241 tcttcggcaa tttgttttcc cgagagcttg aaaatcttca cttgaatatc ttgttcactg
      301 gttgaataga actccacagt gacaccttct ataaacggat taggatacac gtttaccaca
      361 ctatcttgaa ctgcacgatc gagtgctagc tgagatagta tggcgtgagc tttcaagaaa
      421 tcaggaattc caaatccctt tttagcatcc ggagcatcgt actgatcagc actttgttca
      481 atagccttga agatttccat tgcacttgcc tctggatgag cttgccacaa acaagccgac
      541 atgcccgcta aaatgggaga agaaaaggat gtaccgtttg atttacctgg cattcctccc
      601 gaagtaatta cataaggctg ttgccccata gctgttacat tgggtttgat tcgcccgtca
      661 ggagttggac cttgcgaaga gaatgatgca tagttacgat tgacatcaac cgctccgatg
      721 cttaaaacac tgtcgccatc agccggaaag ccaatgtatt tccaaggatc agcgcctgaa
      781 ttaccagcag aattaacgat cagcattccc ttgctagcag ctatatctgc tccgatagtg
      841 atacgagctg ttttgccatc gagttgagaa taggtatgac tagcagttga atcatcaaac
      901 accgtgtagc ccaaagatga atttatcaca tcaacaccaa cactatcggc gaattcggca
      961 ccgcgaaccc aattatcttc ttcaatcaga ttttctgaat cggcatcttc ggtgcgtaaa
     1021 agccaatatg atgctttagg agcagtgccg acaaacacac cgggaatatt tcctgccatg
     1081 gtcgacaaaa cggataatcc atgcgagttg tcttcaaaca cggatgttcc accttgaaca
     1141 aaatcgcggg tacctaataa tcgtccggaa gaacgtaaag aatcgaaaaa aacgatttga
     1201 tctacatgat agaaaccagc atcgagaaca gcaatatgaa ttccttttcc ttgaaagccc
     1261 atttggtgaa gtttatcccc attgagcatt tttatttgtt gatctccatt gccataaaac
     1321 gcatctgcac cttgagcaag taacagtttc ttagctgcat ctgcttcgta ttgctcttgc
     1381 attttcatga ggtcggcaat gaactgatcg gcgggtgatt tttgcttaac gcgagctact
     1441 ttattcgttc tcgctacaaa aggaagagct tcaataattg gcaacaaagt agaatcggtc
     1501 aattcaatgg ttactgcatt aaaccactta ctttgattga ttactcttgc tccagcagct
     1561 tgaatttgtg ccacataagc aggatttaaa ggcaaatcat tttccttaaa accgatacct
     1621 tgttttgccc tacgatcaat agcacgagtt gataaaaatg cttcgggatt tgaaagcgaa
     1681 taaggagttc caaccttgtc ggttaattgt acccaatatt tacgattgaa tgttccgttt
     1741 gtttgagcaa aagcagatag attcagggaa attagaagaa ttactacaag ctgtttcatc
     1801 attgcacacc aatttcgatt gctttcatgg taaatacgac accgctttta atgttagaag
     1861 taggaacaac gtatttcaca tctacatatt tcttgaaaac cattcctacg ttagctgcaa
     1921 atacttcggt agttcgaagt tccgaaacta gattttcggg atcggtattc acaactgtga
     1981 tggttgaatc aaaatcgagt gtaccagttg taaaaggctt atgaacctcg gagaaagtta
     2041 gcgtttgagc cgtaagagaa ttgagtgcgt ttccattcca ttcgattgat tcccgaacag
     2101 ggaaaaccat tttgatgaag cgaacatctt cttccacttt ctcaacctga gcatcggttt
     2161 taacacattg ccaaacatct ttcaagaacc aacttagtgt gtcggcaggt tttttccaac
     2221 gctcaattcg aatagccgaa cgtcctgaat tatcgatgaa tgtagattcg aaatattcct
     2281 ttatcaaaaa ggaattggta tcaacggttt gattaaaatc atcgtaaatg atggagtcgc
     2341 attgatagat gatgtacgaa cctactttgt cgctgaagta gttgcggtat gaaggatcgg
     2401 gcgcaataac cgaatctttt ttacaagaag aaatcagtaa acttaaacag gcaaggctaa
     2461 ataggatgta gcgtttattc atcgtttttt tattttttaa tgaaacctcc tccaacaagg
     2521 tcatttccct cataaaacac agccgattgt cccggagcaa ctcccgaaac agcatctaaa
     2581 aaatctactt gtaacagacc atcaggacgc atattgagaa tgctggaagt accttcgtgt
     2641 ttatatcgaa ttttagtaag tgcttgaaaa tcttcgggaa gctcagcata tttcatcaga
     2701 tttatatccc tcacattcat agtatgcttc accaattctt ctttctcacc taatattacc
     2761 gtgtttgttt cgggattgat atcgagtaca aataccggtt ttcctaaagc gatttccaag
     2821 cctttgcgtt gtccaaccgt gtaaaaagga tagcctcgat gttttccaac cactgttcca
     2881 tccgacaaaa caaaatcacc cccatcaacc tgttcttcta aacctggaac ttgacgtttc
     2941 aagaaaccac ggtagtcgtt atcgggcacg aagcaaatct cgtagctctc gcttttattc
     3001 gctagatcaa caaatccgct gtcggctgcc atttgccgta tttcactttt acgaaatcct
     3061 cctaaaggaa aacgcgtacg ggcaagactt tcctggctca atccccaaag cacataagat
     3121 tgatctttat tcaaatctag accacgcgaa atcacatagc gaccattatc ctgaaaacga
     3181 tgttgggcat aatgaccagt agcgataaac tcacaatcga gcatatcggc acgcttgaga
     3241 agtgcttccc acttgatatg cgtgttacac aatacacagg gattaggtgt tcgtccagct
     3301 atgtattcgt cgacaaaatt attgatgatg tagtttccaa attcaccgcg tatgtcgagg
     3361 ataaagtggg gaaaaccaaa acgcacagcc agacttcttg catcgttgat tgaatcgagg
     3421 ctgcagcatc ccgtttcttt tttagttcca cctgaactgg cataatccca tgttttcatg
     3481 gtgataccaa ttacttcata tccttcttcg tgtaacatca tggctgcgac agaactgtca
     3541 accccaccac tcatcgccac cagtattttt ccgtgcctgc tcattgtccc ggtaatattt
     3601 cgctctcgtc tttctcgagc ttttcaaatt tttggatgtt cggtttgtcc gaaacaccat
     3661 ctttaaacaa ttcagtatcc tttatgttcc cgttctcatc gccgtgatac caagtacctt
     3721 gacgtaaatc attgatataa tttccgctca acttcatctt tccattcgcg tggtagtaaa
     3781 tcattttgcc atggagtttt ccgtctttgt agttggcggt catacgtttg gttgagtcct
     3841 caaaatactg aatccaagga ccttgcttga tatcgtttat gtatgttact tcttccgaaa
     3901 ctggaccttc ggggtagtat acataccaag ttccggtctt catacccttg atatacgttt
     3961 cgcgagataa tacttgtttc tgttcgttgt aataggtcca aacggaatct ttcttttggt
     4021 cgacataatt cccatcagat atcatgtttc ccttggcatc aaagcctttc gcatgtgaac
     4081 gacgaccttt atccgaataa aaacagacca ttttcaattc gcctgtttca tgataatacc
     4141 taaaaactcc tgttggatta tcgtctttga agttgccttc ataacgagga gttccatcgg
     4201 gatagttttt gcgccaaaat ccctgttttt tccctttgga atcggtttta ttcaaggttg
     4261 cttgactact agcggaacta gaaaaaacaa gcacgaataa aaataaagac aggattttga
     4321 gggtgttcat gttaggacaa aaatagggaa tattttaagc agaatggttt agggtttcgg
     4381 aaggagaagt tcaaaaacac gtgtatttcc aacaccatcg cgcgcttcaa tacgaaaagg
     4441 atgatcgcct tcactaagtt catcggggaa tttatacgtc cattcaccat cacttaaacc
     4501 aggctcgagt aaaatccaat tcccatccag gtacgcttgc atggagctga ttccagagag
     4561 cttatccgaa atctgaaccc gaatacgccc gctggctgtt tgtttcaacg gaattttctt
     4621 ccttttcttg cgaatgaaag ctggaattac tttaacactg ggtgaaacag tatcggccac
     4681 aacctgaaaa tcgccaaatt caaatgtttc agcttcgagc cagtcctctg ccctattcgt
     4741 ttttaagcca cgcaagttag ttccgttttt ttgagcaaca accaatttag cttgaagact
     4801 atccggcaca ttagaaggct tgaaacgaag tctgactgct tgatttacag ggatatattt
     4861 actacctaat tcaatccaag gggaaaaaga ggaagggtgt gaaatgagct tgattcgagc
     4921 agtgtcaaac aatgtttctt cttggaattc tgcttgaaaa tcaggcaggc tgatggtttg
     4981 agctaaaccg ggtagaactg gaatataaga tgacgaaact tgagtaggtg taatctccca
     5041 atcaggcttt ttccctcgca ctcgaataga aaagcgagtt tgattattat tcaaatcact
     5101 cagaacatag gtaattgtgt aaatgctatc ctccgaaaaa taataatacc cacgatctag
     5161 ttgcttttta taaatcccaa tcattgggtt atctggtact acgcaacgtt gaatttttct
     5221 gcgtgttttc aaacgttccg gataatcctg ataggcattc acgctgcggg tttcatcgaa
     5281 cgaaaactca tcaaaccgag cttgaaaaac ttccgtagaa tcaactaaca attgaatttt
     5341 atatatcccc gttttgcttc gaagtttacc aatcacatca cctccttgaa atccaaatcc
     5401 aacccaacca gaaacaatag gcatttttat tttgggtgaa attccctttt tcttggtctt
     5461 tttattgaca accagaggaa gattcaaatt ggttttcagg ttattcacac ggccataatc
     5521 gtccatcggg aagattgata cggcattgat ttcaggagca aaatcatcct tttcggcata
     5581 tccaaacaaa agaggattga ggacattttc gaatccttga tcacgaatct caaaatgcag
     5641 atgatgagca gtagaagatc ctgaatttcc actccaacca ataaattgtt gtcggcttac
     5701 tggaaaatca agaggatcgg gcaaaacatc taattcatac tcgcgacttt gctgctggat
     5761 tgtactaaca tattggttaa tcgtatcgtt aaattcggca agatgtccgt aaacagtagt
     5821 taatccgttc gggtgattga tgtagatggc gttaccataa cccgattgag aaacctttat
     5881 acgactcaca tatccttcgg cagcagcaag aacagcttga ttctttcctt gggttgaaaa
     5941 atcaatacct gaatgaaaat ggttgggtcg aatttctcca aaaccagccg ttacttgaag
     6001 aggtatcgac ataggtgaag aaaaaaactt ttcaggaacc tgttgtgcat tcgctaaaca
     6061 ggctgatatc aggcatatta aaaaaatcat ccggctcatc tacagttctt cagcgaattc
     6121 tttgcaaatt attatgagaa aggctctgga agccgcagaa aataaatact tttaagcccg
     6181 ttaattaaca catcaggcaa tcgcattatg acggatctac ttgggaaatc agccgttctc
     6241 gagggcaaaa tcaaaaaatt gatacatttg cacgtcgcgt tcaaagcgga gaattctgca
     6301 ttgaagtctg acaatcaaag attaacagaa accatcaaca agcaacagga gcatatccaa
     6361 caacttgagg aagggaataa agcgttaaaa ctcgctcaag cattgattca aggttccgaa
     6421 aatgaggatc ctacagtgct aaagggcaaa atcaatgaga tgattcggga cattgataaa
     6481 tgtctcgcat tactgaataa atgacccaga acagatgcaa gatgtatcga tagaaataca
     6541 gttagctaac cgaaaatacc ccttaaaggt aaaatcggct gaggctaatt ctgttcaaaa
     6601 ggctgccaca cttatcaacc agaagctcag cgaattggaa actaagtatg gggttaaaga
     6661 cattcaggat ttgtttgcaa tgagtgcttt gcagataact actcagttga ttgagttaga
     6721 gtcgaacaat actggaaatc aggatcaagt atttttccgt ttagaggaaa tggatcagtt
     6781 gattaccagc attttggacg agaaataacg tttccttttt tcggtttagg gttcgttcaa
     6841 gttgtttgtc ataacgtaca aactacatgg aaatactcat ttatgtgatt gtggcaatag
     6901 taggactcgg aggagggtat gcctttgcca cgacggtttt aaggaaaaac attgagcaga
     6961 aaagtgctca attggtaaaa gaagcggaag cggaggcaga agtcattaaa aaagacaaaa
     7021 tgcttcaggc gaaagagaaa tttctgcaac tgaaaacaga acacgagaag tcgatacaaa
     7081 ttcgaaatca agaagttcaa caatccgaaa acagagctaa gcaaaaagaa agcatgctta
     7141 gtcagaaact ggaagagaca aaacgcaaac aaagtgaatt agatgttttg cgtgaaaatc
     7201 taactcaaca gttagatgta attaccaaga aacaatcgga gcttgataaa caacatcgtc
     7261 gccaagttga gcaacttgaa atcattgcag gtttatcggc tgatgatgct aagaatcaaa
     7321 tgatggaagc tttaaaggaa gaagcaaaaa cagaagctgc ttcccacatt aaagacatca
     7381 tggaagaatc tcgattaacg gctaataaag aagctaaacg tattgtggtt caaaccattc
     7441 aacgtgttgc gacagaacat gctgtagaga attcagtttc ggttttcaac attgaaaatg
     7501 atgaagtaaa aggacgaatc attggtcgtg aagggcgtaa cattcgtgca attgaggctg
     7561 caacaggtgt ggaaatcatt gttgacgata cgccagaagc tatcatttta agttgttttg
     7621 atcctgttcg tcgtgaagtt gcacgtttag ctttgcacca attggtaacc gatggtcgta
     7681 ttcaccctgc tcgtatcgaa gaagtggtgg caaaaacgaa gcgtcaaatt gaggaagaga
     7741 ttattgaaac cggaaaaaga acctgtatcg atttaggaat tcatggtttg catccagagt
     7801 tgacacgtat ggttggacga atgaaatatc gttcatctta tggccagaac ttgcttcaac
     7861 actcgcgtga agtagcaaac ctttgtgcga ttatggcagc tgagttagga ctcaatgtaa
     7921 aacttgcaaa acgtgctgga ttgctccatg atattggaaa agttcccgac gatgagccgg
     7981 aattaccaca tgctatcttg ggtatgaagt tggccgagaa atacaaagag aaacccgaag
     8041 tatgcaacgc cattggtgct caccacgacg aggtagaaat gaccagtttg atttctccaa
     8101 ttattcaggt gtgtgatgct atttcaggag ctcgcccagg tgcaagacgt gagattgttg
     8161 aatcgtatat caaacgattg aaagacatgg aacaactggc tctttcttat ccaggtgttt
     8221 caaatacata cgctatccaa gcaggacgtg aattgcgtgt aattgtagaa agtgagcgag
     8281 taacggataa agatgctgaa atgctatcat ttaacatttc gcaaaaaatc caaaatgaaa
     8341 tgacttatcc aggtcaagta aaagttactg tgattcgtga gacacgtgca gttgcttatg
     8401 cgaaataagt tcattgattc aatttcgtat gattgaattt tcatcaaaat ggttaactaa
     8461 atgcattcag acttactatc tgggtgcatt tagtttatgg tacatattgt tcccatttga
     8521 tgctttcgta ttacccatat ctgttgggtt tatgacaatc tatccgtttt tgatcttgac
     8581 tgtcttgttg ttttctgctt ccttttttct tcccaagcaa actaatctcc caacaaatct
     8641 caaattggca ttagttttct ttactctaat tgctttagca ggaattgttc actatagttt
     8701 tgtccaaggt aaatcggaag ctaagtttga aatcagaagt ctgatactgc aagtgttaac
     8761 agtatccatg ctctttcaaa cttttcgttt tgccggttct gagctatttt tcaaaaaagt
     8821 ataccaatta agtatattac tatttgtagg cctttgtttg attggttggt ttgaattcta
     8881 tacaggaatt catatccaag gtttccacac cgaaaaatta atccaattac ctgtaggaaa
     8941 tatcacattt gcacctgttt ttatttacga taacaacaat acatttctgt gttacctctt
     9001 ttctctagct cttataattc tcttaagtag agttggtaaa agacattcat ttccacaagt
     9061 tacttccatt attttccaac ttctcttttt ctccattgtc gctgactcta gatttgggaa
     9121 gattatttcg gttgttttct tcattttcta tatcgcaagt tttatcaata tcgacttcat
     9181 aaagtctaat agtaagctaa ttaaattatc agctttcttt cttttttgcg ttataggagt
     9241 ttttgcattt aactcaattt tcctaggtcc attatggaaa aatggaagag actataaatt
     9301 gaattccatt caggttattt cagagaataa tggtaagcct gtattttcaa gtcccgatag
     9361 tttaattaag gtaattggaa aagaaaaagt acttaaagct tacgaaaaat atgagatgca
     9421 tggcaaactc agctccatat ttattcgaaa aaacctttta ctaaatggca ttttcctgac
     9481 caaagagcat ccactatttg gtgttggacc aggacaatat tcctggtata cgattaaaaa
     9541 taatgtgcct tatgtaactg gaacaattac tagtccacat gaaggtttaa ctgaactcgt
     9601 aagccaatat ggtattccat taacacttct attcttcttc gtgctactgt taactttttt
     9661 acgttttttc aaaggaagaa agttcgattt attcaagact gcaaagtgga ttttatgctt
     9721 ggctgttttt tggatagtag cagccatgcc atccgcttgg ttagtattaa attgtggatg
     9781 gattctaatt gctataattt tactttcacc cgaaaatgta actaaagcaa gtgagcagta
     9841 aatcgtcccg atcgtttact cagaatttct taattctctt tactgggaat tctatcggtc
     9901 aggcaattcc atttcttctt gctcccttta taagccgaat atacaaaccc gaggaaattg
     9961 ccgtacaaga aaacttctta gccatggcat cattaattgc cattgttgcg gccggtagat
    10021 ttgatcttgc tattctcatt cctactgatg acaaaaaagc aaaatcactt tttagtctat
    10081 catttataat tacaacaata gttagtcttt tatcacttat tactttatta tttactaatc
    10141 aaatagctca attctataac actcctgaat tatcccaata cctagttttt ttagcaccta
    10201 gtattttcct actatcatca cttagtatct ataatcaatg ggtacttcga aataaaaaat
    10261 acccgctact aaccagtatg cgcgttcttc aatcactcgt acaaaactct ggatatgtag
    10321 ttttggggta tttaggttgg ggaatttctg gtttattttg ggcatggata attggcttat
    10381 tccttccaat agcatggatg acgattcaag aaagaggcat ttttaaatca ctttctaatt
    10441 cccgaaaaga acttaaagat gtagcgtacg aacaccgtga ttttcccatg gttaattcga
    10501 ttcatgcttt taccgatata ttagctacac agttcttgct ttactggctt attacacaca
    10561 attacggagc tttagcattg gggctttttt ctgtcatgaa ccgttattta cgtgcacctt
    10621 taaatcttgt aggaagcgct gttggacaac tgtatttccg tgaagttggt gctaaacaag
    10681 gagaaagctc agaacaacta aaattgtttt accgttccat tcgaattatt tcactagtaa
    10741 tagtcccagc attagtttta gttcttttca aaggaccatg gatttttgaa atatacttgg
    10801 gcgagaactg gcgtgaagct ggagtactag cacaaatcat ggcacctgca attttattca
    10861 atttcatagc ctcggttgtg tccactacac cattaatatt tcacaaacaa aaaccagcgt
    10921 atgtcattgg tttaactgga catatattga gtttaggaac attattcata ggctcactca
    10981 caacgtataa tttccatcaa atattgatgg cttattcggg tgttttaagc ttgaattata
    11041 tatttctcat tttctggtat agaagtctaa tccccaaagc agcgatatga acgtagttgt
    11101 aaacggactt ccatatttct ccagaaagct agttgctgat ttacaaggat ttgcgccaca
    11161 gcatcgtttt cgcttttacg atacctataa ttcgaaaatc gagcaggtga aattcgcttg
    11221 ggcaataaga aaagccgatg ttttaatttc gataaatggt gtctctgatc gaagcggttc
    11281 gatggatttg gctttaaaga tgaacaagaa aatctggttt aactgggttg gaacagatgt
    11341 acttcttgct gtggaacgat ttaaaaatag ttcaatattt cgtgattaca ttgatcgggc
    11401 agttcattca accgatgcgc cttggttaca agctgaacta aaaacggctc aaatagaagc
    11461 taaaatagtt cactccaaat ggttagatat tcagtcaaat gcgagtgatt tcccaagact
    11521 acaagtttat tcatacgtgg ctgctaacag agaggatttc tatggctgga ataccattca
    11581 acaacttgcc cttgctaatc ctgaaatccc tttcattata gcaggaagtg atggaacaca
    11641 attgaattca attcctccga atgttcaatt taaaggctgg ttaactactc aagaaatgac
    11701 tgagcttcga aatgcttcgc ccattttcat ccgacttgct gaacacgatg gcttctcctt
    11761 aagtgtgctt gaagctctag ctgctggatg cgaagtaatc tggaaatatc cgttcgaaaa
    11821 ttgtcatcat ttgaaggata tagaagttga ttttagcaag gttgtgaact tagttaagga
    11881 acgtaacttc acccctaaca aagaaaacat cgcgtacatt gctaagaact ttcaaaagga
    11941 agtgatattg aataatttca ttcaagaatt ggagaaaatt gtcggataat cgaatacata
    12001 ttgccctagt tacagcttgg tttccgccac gtaaaagtgt tgcagtaaat cgaatgttag
    12061 catttgttaa ttacatatcc aaagagcgtt ttaaaataac tgtcatcacg ttgaatgaag
    12121 gcaattctcc agcacatgag acgaatcaaa ccggcgatat ttatcgagaa caagacgaac
    12181 aaataattcc gttcttaaaa aataaacagg gagaaaataa actacttcat ttatccaaag
    12241 tagtttggaa tgttttgcta ctaaaggttc ttcaataccc gcataaagcc tggatgaaac
    12301 gagttgaaaa gcgacttgaa ttcgttcatt caaaaaaccc aattgattta attattagtt
    12361 cattttcacc cattgaagca catttagcta cgtacaattt tctgcaaaat cacaagtcaa
    12421 ttgcttgggt tgctgacatg cgtgatgaga tgtcgacgaa tggacaaata aataatcaaa
    12481 ctcgaaaact ccttcagaaa gcagaaatta aaataaacag tagagctcaa gcaattacaa
    12541 ccgttagtag tcccatactt aatgatatga gacgaattat gacgcaagtt caggagtttg
    12601 cagaaattcg gaatggtttc gataatgatt taaaaccaga tcgcaatcat aattccgtat
    12661 tcacaatcat ttacgcggga acattttacg gaccgagaaa accgcataca tttttcgaag
    12721 gattgagaat ttttctttct actcacacag tagagttcaa aattcaattc ttaggtactc
    12781 accataattt ttctattccc aaagaatttg aaaataaatg tgaattcatc cctccggttg
    12841 ataataataa agctctagaa tacatttcaa aagccgatgc cagcttattg attttaaatg
    12901 aaataaatag agttggtgtg tattcaggga aaatatttga ctatatctcg gtacaaaaac
    12961 caattatttc ggtattaaat cccaatgatg ttgctgccga tttaattcga gaacaaaatg
    13021 ctggttatat agctgatttt aatcaaccaa atgaaattgc tcgttcgatt gaatttgctt
    13081 ggaaaatatg gaacgataag agttatttac ctgttgatga agcaaaagtt aaaacccttc
    13141 atcgtaaata ccaggttaaa atattagagg atctgatttt aaaaattgtt tcgaaatgaa
    13201 cgttttacaa attggctccg actccattca cctccggcat ttctgtaaag caatgtcgaa
    13261 attggtaggt aatttaacct tagtcactga aacaccattc tctccagaag gcacaaataa
    13321 aaatctggtt tacccattta gaggattgaa tttattgggt tggattaaaa attaccattt
    13381 actgaaaaaa gaaattcagt ctataaaacc cgatttaatt catattcacc aagtaaatcg
    13441 acttgcatgg attgtatgtc gaattgcaaa atctcttaga ataccagtaa ttacaactgc
    13501 ttggggaagt gatgttctca tcattcctca aaaaaacagc ttgtattatt ccatcactaa
    13561 atcggttttg gattatagta cttatgtcac tgccgattct catgaaatga ttagggcaat
    13621 gcagacaatt tcgccagcag ataaaaaata tgtttatctg caatatggaa ttgacccagt
    13681 taaaccacag ttgaaacaaa gaatcattta ttcaaaccgc ttgcataaag agctctatcg
    13741 aattgattta attatcaatc ttttcagtga gttctataaa caacatccgg attggaaact
    13801 cattattggc gcaacaggaa ttcaaactga ttcattaaaa gaacttgcag caaaactact
    13861 gccttcatca gcctatgaat ttattggttg ggtagatcaa gaaaccaatg ctaactttta
    13921 ttcactagct cagatttaca tttcccttcc caatagtgat ggaacatcag ttagtttgct
    13981 tgaagcgatt tcggctgact gtatccctgt cgtgagcgat ttagaagtaa gtagagagtg
    14041 gattacaaac caagaaaatg gaataatcta ccattcttct gaaaaccctt ttgagcaagc
    14101 attgaatgtt gattcagaaa aatgctttca aataaatcag gaactcatta ctaaacatgc
    14161 acttcgagca acgtgcatga ataaatttga ggaattatat ttcaagctga aggctatatg
    14221 aattggaagg aaatcatagc tcagccatta ctcattttaa ctctgttatt agcggcggtt
    14281 cttcctttat cacaacccct aattccttta gtaattgttt tattactcgc tttttatagc
    14341 tggaaaggac attggaaatt tagctgggat ttagttagat catcgcctga aatccttgct
    14401 ttacttggct tctatgttta caatgtgctt ggcttaatag gcactgaaaa ttattcaaag
    14461 ggatttattc atttagaaat taaattatcg ctattccttt tgccttggtt gatttttgcc
    14521 agatctgatt tgaatctatc caagaaaaaa ttaattaatc aaacctttat tggggcttgt
    14581 ttaatctatt gcatttggct gcttggccga tcaacattta tatatattac aaaaggtcaa
    14641 aatgaattct catatgagtt attctcgaat ggatttcacc cttcctattt agccatgtat
    14701 ttactgcttg ccattcgctt gtgttacgca gaaatacaat cgattcaaac aaaattaatt
    14761 atcccttatt tgctcataat agccgtattc gttagttgca ttgttttatt atcctctaaa
    14821 ataaatctct ttttactcgt attttccttc ctatattttt ttgtggaagg atttatccgt
    14881 gcaaaacgaa aaacctattg gatatataca gcagctgctt tcataggaat aatagcggtt
    14941 tcagtttttt ataatccaag tatttccgaa agagccgaac gaagttttca agtattttcg
    15001 aatttaaata cggttgataa atccgatact gaaagtaatg ctgcccgcat tctaatttgg
    15061 caatcggctt ggacattaat gaaagaacat cccatgggcg ttggtgttgg agatgtaaat
    15121 attgaattag aaaatcaata tcgacttgat ggatttaccg gtattgaatc caagaaatta
    15181 aatgcgcaca atcaattttt acaaacaggt gttgctttgg gatttccagg attaatttcc
    15241 ttattactac tcttcattct tcctattatt tctcgtaaca aaagacaatc gggaatattg
    15301 atgtatttcc ttgttatttg ttttgtgaat gctttggtgg aaggaattct tgaaactcaa
    15361 aagggtgttg tatttttcgg cttcttttac tgtctattat taaaagaaga tgagcgcatc
    15421 taaacgggta agtgttgtaa taccatgcag aaacgaggaa aagtacattg ccaaatgtat
    15481 cgaatcggta ttggcaagta actacccaag cgatatgtta gatgtgtttg tttgcgatgg
    15541 aatgagcact gattcaactc gagaaatagt taaaaatttc catgaaaaca atcgtgttac
    15601 cctattagat aatcttcaaa taactactcc ttttgcgtta aatctaggca ttgaaaaatc
    15661 cttagctgat atcatcatca ttttaggtgc acacgccgaa ttagataaag actatgtgaa
    15721 actttgtgtc gaaacattcg aaatcgaccc cgagataggc tgtgtaggtg gaatcttgga
    15781 cactatttca ttagatgaga attcggcagc aattgccctt gccatgtcat ctgtttttgg
    15841 tgtaggaaat gcgcatttta gaactggatt aaaagctgga tacgttgata cggttgcttt
    15901 tggtgcgtat cgaaaagaag tattcgaacg agttggatta ttcgattcag ccttaactcg
    15961 aaatcaagat gatgaattta atttccgcct cattcaaggt ggatttcgta tttatctgaa
    16021 cccaaacatt cgggcaaaat actacgttcg ttcaaccttt tctaagctgt ataagcaata
    16081 caagcaatat ggttattgga aagtgtatgt gaatcgaaag ttcaaagttg taacaacact
    16141 tcgacaatta gctccaccac tttgggtgct ttttctgatt gcattgccct ttgcatggct
    16201 cattcatcca atattgggga caattcattt aattttgctc ctgctttatg tgcttatgtc
    16261 tttgcttatg agcttaagaa ttagtcggca cgtaaaggaa tttggtct
//
LOCUS       DAOOQW010000037        15933 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_4464_length_15933_cov_6.026830, whole genome shotgun sequence.
ACCESSION   DAOOQW010000037 DAOOQW010000000
VERSION     DAOOQW010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 15933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15933
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4464_length_15933_cov_6.026830"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /gene="ccsA"
                     /locus_tag="PK281_03905"
     CDS             complement(<1..480)
                     /gene="ccsA"
                     /locus_tag="PK281_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014200578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HOY48519.1"
                     /translation="MSKSPDSASFLSRNWWKMLGVCLVFYSIIMGFLGTVPRLPILHE
                     TIRNLYFHVTMWFSMIFILGASLVFSIRYLSNGRFEDDIKAEHYSHVGILLGMLGITT
                     GSVWAHFTWGAWWVNDPKLNGAAISLLIYFAYIILRNSMDEEQKRARVAAVYNIFAFV
                     "
     gene            622..1065
                     /locus_tag="PK281_03910"
     CDS             622..1065
                     /locus_tag="PK281_03910"
                     /inference="COORDINATES: protein motif:HMM:NF036614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5362 family protein"
                     /protein_id="HOY48520.1"
                     /translation="MQYDSPLDSNQRNQTTSIDELALSYLLKTSKWSKFLAILGFVLI
                     GLIVLLAIIMGTVLGNTIPNVPGFVITIVYLVIAGILVPPMLSLMRFSNFIQQAVSQR
                     DNSMLTEAFGQLHSHYRFIGIMNIVVIGLYILAFLGGMASAAFMR"
     gene            complement(1111..2442)
                     /locus_tag="PK281_03915"
     CDS             complement(1111..2442)
                     /locus_tag="PK281_03915"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOY48521.1"
                     /translation="MKLLRSIQPTARFNYSLGIIAGLFALSFAWEIVFPIAQIALALL
                     LAFTIGEGVMLYVTGIRFQASRVVPRILSLGAKQEVQLSIANLSSQILHAEIIDELPE
                     QLQMRDFSMKANLSGGSIVRLSYSIRPLKRGLYRFGNVILFVQTRIGLLKRRFEIELS
                     KELAVMPSVAEMKQFELRTFADLNSTRGIRKIRRIGHSYEFEQIKEYVSGDEYRSVNW
                     KATSRRGKLMVNQYEDEKSQQIFSVIDQSRSMELPFDGLSLLDYSINTSLVISNIALR
                     KQDKAGLIHFSSHAVTVVPAERSRSHMKRMLEALYQLAEKDLEPNYELLYTAVRGRIS
                     SRSLLFLYTNFESQHALERALPLLRKINKLHLLVVIFFENTDISDLAQQAGKNLEEIY
                     IQTTARKHVTMKLGLVQLLRQHGIQTIYTRPADLSVNTVNKYLELKARGLI"
     gene            complement(2442..3491)
                     /locus_tag="PK281_03920"
     CDS             complement(2442..3491)
                     /locus_tag="PK281_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008612987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOY48522.1"
                     /translation="MSTENETPESTGLPETNSPEQNEAANPLAEGPELAVLPVMQAAD
                     AIRDEIGKVLIGQDELVTQLLTGLFAGGHLLLEGVPGIAKTLSAKLLAKTLQLDYSRI
                     QFTPDLMPSDVTGTSVFNLKTSEFTFNRGPVFANVVLIDEINRAPAKTQAALFEVMEE
                     QQITVDGTTYDLASPFIVLATQNPIEQEGTYRLPEAQLDRFLFRIRIEYPNLNQEIAI
                     LSRFRDDFNKSYLNNLKSILNPDDIAKIRQLVEQVYIKDELLQYIAQIVHQTRNDSDL
                     FLGASPRASLAILRASKAFAAINGRSFVIPDDIRQVAIPVLNHRIQLSPDREMEGLDT
                     IQVIKRILQQTEVPR"
     gene            complement(3488..4744)
                     /locus_tag="PK281_03925"
     CDS             complement(3488..4744)
                     /locus_tag="PK281_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48523.1"
                     /translation="MKKSPNGLLIGFLALVLVGIFIYASNRSSPYFNWYETYKPEDKQ
                     PYGTYVIREILESYNPNFKFKELTKSWKEDSLKSSDKGIYFFVGKEDYLFEEEQHQLL
                     NWVAQGNTAFISAKAFPNLIMSELYDSLCINANNSNTFYFYDTLVKANFYHPELREET
                     GIPFHFQENDKRVSYDFGCFDSTQFCEQSKFEPLGYFEPNYVNFIRVKHGSGMFYLHT
                     SPIQFTNYHLIQASRANYAAKVLSYLPQASIYWDEYHRVEHDEQGMEKMEAPNETPLS
                     FILANKSLRWSLYVILLLALMYLLFNTRRKQRVIAVIESPRNSSLEFVQAIGRLYFHQ
                     RSHPTLLRHQMRYFYSFIREKYRIPIQELNEENIRLISQRSGVPITLLQSIRDESTRL
                     QPYVELSDKEILAFYKILSQFYSLAR"
     gene            complement(4741..5589)
                     /locus_tag="PK281_03930"
     CDS             complement(4741..5589)
                     /locus_tag="PK281_03930"
                     /inference="COORDINATES: protein motif:HMM:NF024948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HOY48524.1"
                     /translation="MKQIHSILVGLCLLFCQLLTAQQSDTVVADSVITYDDSTADYDD
                     EDETISINDEEGIFDGRPAGFSIDSLSRQYKNEKTGKKQFDERLYQRAIQGLDYKEPP
                     QPKKEEKKLPKQEIETSGKSSFLGIGQVVLIIVILIVLVVLIMLIINQAKQTNPRLKE
                     DPDWLQVDIEKEASPEQILQQKLDECLRNGNYAFAVRLLYLQTLADLHREKFIRWKKD
                     KTNADYVAELRNTPLFRPFRDLTRWFEKSWYSHTPPDEKTYRKLEPRFMQFRQEIGRN
                     QEGRRE"
     gene            complement(5586..7427)
                     /locus_tag="PK281_03935"
     CDS             complement(5586..7427)
                     /locus_tag="PK281_03935"
                     /inference="COORDINATES: protein motif:HMM:NF014052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HOY48525.1"
                     /translation="MKETRFIEQNKDKWIELERMLSESKRDPDKASELFVQVSDDLSY
                     ARTYYGNRVVRVYLNQLTQKVFGTLLRNTVNHKNRFSYFWTDDLPAVIYHSRKELIFS
                     FVLFVFCMLIGALSSHYDTNFAQTILGADYVSMTNSNISSGDPMAVYKDKDALNMFLY
                     IAQNNLIVAFRTFLFGLLFAIGTIGSTVSNGIMVGVFQYYFISRGLLVDSALTIWMHG
                     TMEISAIIIAGGAGLVMGKGLVFPGTFTRLQALRISARSGIKIMAGITPIILVAALIE
                     GFITRYTELDNFIRLGFILLQAGFIVAYFVIFPFRKSRSGVPFRLDEQKIPQTPKFAP
                     EMDQVKSSSIIFGDSFSLFIRILSKNWKYLLGISLLYTISLLLLLPEDKILNISYNTW
                     FINKIPEILPFNFKYSFAELFIIGGALAATIFGNYWRSESLLLAPGETRSKTGFKFQL
                     SKFGGSVLILSILVLPLIPDIGFFYLLFYLILPFMLLWLAGWQVSELSFGAATSLIFK
                     NAFENLMRHYGTFLSLGLIAIFFFLLADLILPLMDTSVLTWNFKVGDFTYYWIKVGTI
                     VFLQTTALFLVIPLFLSGMSFSFYSCREISEAPGLFERLRKAGLVNE"
     gene            7484..8218
                     /locus_tag="PK281_03940"
     CDS             7484..8218
                     /locus_tag="PK281_03940"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HOY48526.1"
                     /translation="MRRIEIITPQNVPILYQLASVRERMLAFLLDILILLITLYIIYL
                     ICASSFDEDTLMTITFLFLLPIFMFYSLGMEVFNNGQTIGKKAMRIKVVKLTGLELSL
                     SDYLLRWAFRWIDIWGSLGSIAALKVSSSTRGQRIGDLLADTTVVRTNADFTVSLQEL
                     LAIKSTTDHEIAYPGIKRMKEEEMLLVKMVLDQYKRFPNPAHHRVIEQTAKGIAERID
                     LDHVPKDKQGFLIRALTDYVTLTRSA"
     gene            complement(8225..9691)
                     /locus_tag="PK281_03945"
     CDS             complement(8225..9691)
                     /locus_tag="PK281_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48527.1"
                     /translation="MKQSSKVFYPYVDTAGLPFNFTDLYGKLSFNGDNGSKLNLTGFN
                     FSDRVKYQSLQDLHWNTFGAGANFVLVPGNSNFVMDGVFSYSDYKINLDQQTLPSRSS
                     EIKGFNLALNFKYFFGKNDLLWGVEAIGLNTNYTFFNELNRKIEQDVNNTELALYAKY
                     HFTVGKLVAEPSFRLHYYASLGEASPEPRLGLKYNVTDNFRLKFASGLYSQNLIAGVS
                     DRDVVNLFYGFLTAPDNTPTEFKGETRTSKLQKAQHVIFGFESNLTKHIKWNVEAYYF
                     NFNQLTNINRNKLYDDNAENEDKPDVQKKDFILEKGISKGIDMTLKYDYKRFYIWAVY
                     SLMKVTREDELIDYSPVWDRRHNVNLLASYEFGKGLNWTFSARWNYGSGFPFTQTQGF
                     YENINLSSNINGDYTSANGTLGSTYGALNQGRLPAYHRLDVNLKRTVVLGENTTLELT
                     AGVTNTYNRDNIFYFNRVKFQRINQLPILPSVGVNLRF"
     gene            complement(9688..10524)
                     /locus_tag="PK281_03950"
     CDS             complement(9688..10524)
                     /locus_tag="PK281_03950"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOY48528.1"
                     /translation="MLNRYFLAFFFSIVGTLLLAQTATIRGVVYNKKNGEPVIFTNVH
                     LDGTQFVAQTDVNGFYTITKVPAGSYTLVFSSIGFEKTSVNVNVKAGQILTQNLYVQS
                     KAVDIKEFEVRGEKEEEQNRIGVGLTTITPKEMNKLPTIGAEPDIAQYLQVLPGVTFT
                     GDQGGQLYIRGGSPIQNKVLLDGMIIYNPFHSIGLFSVFDTDVIRNADVYTGGFSAEN
                     GGRVSSIMKITTKDGDKKRFGGKVSASPFVSKLQLEGPLSRQKDGKASSSSSSLADDL
                     PI"
     gene            complement(10663..10938)
                     /locus_tag="PK281_03955"
     CDS             complement(10663..10938)
                     /locus_tag="PK281_03955"
                     /inference="COORDINATES: protein motif:HMM:TIGR02116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HOY48529.1"
                     /translation="MTIEFTELAWEEFSFWVEHDPVIVNKIKELIQAIRKDPFKGIGK
                     LEPLKFGLKGFWSRRISGEHRLVYQITGTKGINQKCSIIQCKLHYDV"
     gene            complement(10951..11202)
                     /locus_tag="PK281_03960"
     CDS             complement(10951..11202)
                     /locus_tag="PK281_03960"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HOY48530.1"
                     /translation="MKAISISLLRKGMKEYFDYVTKSMDVIIVPRAKEEEAIVIMSIQ
                     EYNSLKETEHLLSTQANQNRLQKSIKQLEKSKTIPYKID"
     gene            complement(11316..11789)
                     /locus_tag="PK281_03965"
     CDS             complement(11316..11789)
                     /locus_tag="PK281_03965"
                     /inference="COORDINATES: protein motif:HMM:NF024976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HOY48531.1"
                     /translation="MGPTNLGLRVLLLPAITPLTREVAGQRTQIFDAFRNKYVALTPE
                     EWVRQHVLHYLLDELKYPKNKIAVEVSLKVNGLSKRADIVVYDSEMNPWMLIECKAAE
                     VEVNQSVFDQAARYNLSMKVPFLVVTNGMRLLAARIDLKKGKIFPVKGMPVYGDE"
     gene            11788..12561
                     /locus_tag="PK281_03970"
     CDS             11788..12561
                     /locus_tag="PK281_03970"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HOY48532.1"
                     /translation="MKSKEEIVNNWLPRYTGMQLEDFGKYILLTNFSNYVEKFAAITG
                     AKVVGLDRPMPCATANGITIINFSMGSPNAAIIMDLLSAIKPKACLFLGKCGGLKKKN
                     KVGDLILPIAAIRGEGTSNDYFPAEVPALPAFNLQKAVSTTIRNHNKDYWTGTVFTTN
                     RRVWEHDNDFKKYLQKIRAMAIDMETATIFSVGFYNKIPTGALLLVSDQPMVAGGVKT
                     EKSDSAVTTNYVDSHIQIGIDSLNELINNGNTVKHLRFE"
     gene            12595..13617
                     /gene="holA"
                     /locus_tag="PK281_03975"
     CDS             12595..13617
                     /gene="holA"
                     /locus_tag="PK281_03975"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HOY48533.1"
                     /translation="MAKSSTSSVSVESVFKELQARKFAPVYMLCGEESYYIDQLSNYI
                     ENNVLTEDEREFNQTVLYGIDVTADDVMATAKRYPMMSEYQVVIIKEAQQLRDIEKLV
                     PYLNNPQSSTVLVICYKGKEPDKRKEFYKSLLKNDAVYFVSERPRQEKMPEWVNRYFT
                     KKGYRITPNAAILLSEFVGNELEKVCNEADKLFLNSPAGSEINEKDIERQVGISREFN
                     VFEFQKALGTRNVFKANQIALNLAGQKATPLPMVMGSLFSFFSKLLHYQWLKSKGETN
                     MASEMGISPYVMKDYEMAARNYPPFKLLRIMSHLRNYDLKSKGLNNESANETELLKEL
                     TFKIVH"
     gene            13617..14021
                     /locus_tag="PK281_03980"
     CDS             13617..14021
                     /locus_tag="PK281_03980"
                     /inference="COORDINATES: protein motif:HMM:NF013995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HOY48534.1"
                     /translation="MNYWLVKSEPVKYAWSKFVKDKNTVWDGVRNYQARNNLQAMKLG
                     DLVLFYHSNEGMNVVGIAKVVKEAYNDPTTDDLRWFVVELAPVEALKKPVSLEQMKSD
                     EKLANIGLIRQSRLSVMPLTNIEFDRILELGS"
     gene            14130..>15933
                     /locus_tag="PK281_03985"
     CDS             14130..>15933
                     /locus_tag="PK281_03985"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HOY48535.1"
                     /translation="MFIYKYFSKRNINPFFYLVPLVFLVVSCSNSSNESKQNATHVAE
                     VDSYSVPQKTILATLPDSAQVKTILLADQEKLIHTNIPRNGASARAAVNMDGKAQQIL
                     VESPITKPLSYTKNGQGQINLDRNGKPFILGNGGRASFKQFTSETGLAMDVVVCAFTD
                     ASGNIWFGTLGGGISKYDGKSFTNYSTAHGLPNNQIYGITQDKSGRMWIATLGGGISC
                     FNGKNFTTYKTEQGLASDFVNCILVDKNGLIWSGTRDGKVSSFNGKKFTNYNLAKLPG
                     DDVINSIYEDLNGNLWFGAEISGLHRFDGKQFKNYTTTQGLAGNVVRCIKGDSKGNIW
                     IGTFDNGISCIPAQQNNQAGKAIIFKNYSSQDGLSENDVFSMHVDHLDRVWFGTDGGG
                     VNCLNPESIADSSKGRLFTCYNSELGLANNSVFGITEDLNGHIWLCTYGGGVVRFDGE
                     AFTVFSEKQGLAANNIICINQDKSGNLWLGSIGKGASCFDGKSFTNYSPIQGLAGSVV
                     TCMMNDRNGNMWLGTDNGGAVLYDGKSFTSYTVAHGLSSNYIISLYQDRSGAIWIGTV
                     GGGVCRFDGNSFTSYSTPQGLAGDMVMSILEDKSG"
BASE COUNT         4817 a         3138 c         3233 g         4745 t
ORIGIN      
        1 cacgaatgcg aagatgttgt ataccgctgc cacacgagca cgtttttgtt cttcgtccat
       61 cgaattacga aggatgatgt aggcgaaata gattagtaat gaaatggcag ctccattgag
      121 tttcggatcg tttacccacc aagcgcccca ggtaaaatgt gcccaaacgc ttccagttgt
      181 tattcctaac atgccaagaa gaattccaac atgcgagtaa tgttcggctt tgatatcatc
      241 ttcgaaacga ccgttcgaca aataacgaat gctaaaaact aatgatgccc caaggatgaa
      301 aatcatcgag aaccacatag ttacgtggaa atagagattt cgaatggttt cgtgcaagat
      361 gggtaggcga ggtacagtgc ctaagaatcc cataatgatg gaatagaaaa ccaaacaaac
      421 acccagcatt ttccaccagt ttcgtgatag gaatgatgct gagtcgggtg attttgacat
      481 agaagttgat tcgattgacg cggcaaaatt agtcattatt gcaatcggtt gcttggtgga
      541 aaaggatcta ataaatccac aatcttacca gagcctaaca atcaattttt ttacatattg
      601 tagaaaaact atctaatcaa tatgcaatac gattctccac tcgactccaa tcaacgaaat
      661 cagactactt ctatcgatga attagcatta tcatatctat taaaaacctc taaatggtcg
      721 aagtttttag cgatacttgg ttttgtccta ataggcttaa ttgtcttatt agctatcatt
      781 atgggaactg tgttaggaaa caccattccc aatgttccgg gtttcgtaat cacaatcgtt
      841 tatttagtaa tcgctggaat tcttgttcct ccgatgcttt ctctcatgcg attttctaat
      901 tttattcagc aggcagtttc gcaaagagat aattcaatgc tgactgaagc ttttggacaa
      961 cttcattcgc attaccgttt tattgggata atgaatattg tagttattgg tttatacatc
     1021 ctagcttttc ttggaggaat ggcttcagca gcgtttatgc gttaatccta tggctgaaaa
     1081 tcttcggact aaagtccgag gttgcgtgag ctagattaat ccgcgagctt ttaattcgag
     1141 gtatttattg acggtgttga ccgataaatc cgccggacgt gtataaatgg tttgaatacc
     1201 atgttggcgt agcaactgaa ccaatccaag tttcatagtt acatgcttac gggctgtggt
     1261 ttggatgtag atttcttcta agtttttacc tgcttgttga gctaagtcgg agatatcggt
     1321 gttttcgaaa aatatcacga ccaacaaatg cagcttattg attttacgaa gcaatggtaa
     1381 tgcgcgttcc agcgcgtgct gactttcgaa attggtatac aaaaacagca aactacgcga
     1441 agaaattcgt ccacgaactg ctgtatataa taactcgtaa ttaggttcta aatccttttc
     1501 cgccagttga tacaaggctt ccaacatacg tttcatgtgc gaacgtgagc gctctgctgg
     1561 cacaaccgta acggcgtgac tcgaaaagtg aatcagtccg gctttgtctt gctttcttag
     1621 ggcaatattg gaaatcacca aactggtatt aatcgaataa tcgagcaaac tcaagccatc
     1681 aaaaggcaat tccatcgacc gactttgatc gatcacgctg aaaatctgct gcgatttttc
     1741 atcctcgtat tgattcacca tgagttttcc ccggcgactg gtagctttcc agttaacact
     1801 tcggtactca tcaccactta catactcctt gatttgctca aactcgtaac tatgtccgat
     1861 gcgtcgtatt tttcggattc ctcgggtaga attcaaatcg gcaaaggtgc gaagctcgaa
     1921 ttgcttcatt tctgcaaccg atggcatcac tgctagttct ttgctcaatt caatttcgaa
     1981 gcgacgtttg agaagaccga tgcgggtttg aacaaacaaa atcacattcc caaaacgata
     2041 caaaccacgt ttaagcggtc gtatagaata gctaagtcga acgatagatc cgccggataa
     2101 attagccttc atgctaaaat cacgcatctg caattgctcc ggtaattcgt cgattatttc
     2161 ggcatgtaga atttgggatg aaaggttggc aatcgacaat tgaacttctt gcttagcacc
     2221 taaggataaa atccttggaa caacccgtga agcttgaaaa cgaattcctg tcacatacaa
     2281 catcacccct tcaccaatcg taaatgccaa taaaagggca agtgcaattt gtgcaatcgg
     2341 aaaaacaatt tcccaggcaa acgacagagc aaaaagtcct gctattattc ccaaggagta
     2401 gttaaaccga gcggttggct gtatggagcg aagtaatttc attaacgagg aacttcggtc
     2461 tgctgtagga tgcgtttgat tacttgaatg gtatctaaac cttccatttc acgatcggga
     2521 cttaactgaa tgcggtgatt gagtaccgga attgccacct ggcggatatc atctggaatg
     2581 acaaaagaac gtccgtttat agctgcaaaa gctttcgaag cgcgcaaaat agctagcgat
     2641 gcacgaggtg aggctcctag gaataaatcg ctgtcgttac gtgtttgatg cacgatttga
     2701 gcgatgtatt gcaataactc atctttgatg taaacctgct ctaccagctg acgaatttta
     2761 gcaatatcat caggatttag aatcgatttc aagttgttga ggtacgactt gttgaaatcg
     2821 tcgcggaagc gggaaaggat ggctatttct tgatttagat ttggatactc gatgcggatg
     2881 cggaaaagaa aacgatcgag ttgagcttct ggaagtcggt atgtaccttc ttgttcgatt
     2941 ggattttggg tagccaacac gataaacgga ctcgctaaat cgtaggttgt accatcgacg
     3001 gtgatttgtt gttcttccat cacctcaaac aaggcagctt gagttttagc tggggcacga
     3061 tttatctcgt cgatgagtac cacattggcg aaaacgggac ctcggttaaa ggtgaattcg
     3121 gatgttttaa gattaaacac actcgtaccc gttacatccg aaggcatcaa atcgggcgtg
     3181 aattgaatac gtgaatagtc gagctgaagc gtctttgcca gcaatttggc acttagcgtt
     3241 ttagcaattc cgggcacgcc ttcgagcagt aaatgtccgc cggcaaataa tccggttagg
     3301 agctgtgtta cgagttcgtc ctgaccaatc aacactttac caatctcgtc acgaatcgca
     3361 tcagcggctt gcataacggg taaaacggcg agttcgggac cttctgccag aggattggct
     3421 gcttcattct gttcaggtga attagtctcg ggaagtccgg tagattcggg tgtttcgttt
     3481 tctgtgctca tcgggcaagg gaataaaact gggatagaat tttataaaat gcgaggattt
     3541 ctttgtcgct taattccaca taaggttgta agcgggttga ttcatcgcga atggattgta
     3601 aaagggtaat tggaacaccc gaacgttgcg aaatgaggcg gatgttttct tcgttaagtt
     3661 cttgaattgg aatgcggtac ttttcgcgaa tgaagctata aaaatagcgc atttggtggc
     3721 gaagtagggt agggtgactt cgttgatgaa agtacaaacg accaatggct tgtacaaatt
     3781 cgagtgagct atttcggggt gattcaataa cggcaataac acgttgtttt cgtcttgtat
     3841 tgaaaagtag atacatcaaa gcaagcagta gaatcacata taaactccaa cgaagcgact
     3901 tatttgccag aataaaacta agaggcgttt catttggagc ctccattttt tccatgcctt
     3961 gctcatcgtg ctctacgcgg tgatattcat cccaataaat acttgcttga ggtaaatagc
     4021 tgagcacttt tgctgcataa ttagctcgac tcgcttgaat gaggtgataa ttggtgaatt
     4081 gaatcggact tgtatgcaga taaaacatac cagaaccatg ttttacccgt atgaaattca
     4141 cgtagttagg ctcaaaataa ccaagtggtt caaacttgct ctgttcgcaa aattgagttg
     4201 aatcgaaaca gccaaaatcg taggaaacac gtttgtcgtt ttcttgaaaa tggaatggaa
     4261 tacctgtttc ttctcgtaat tctggatgat agaaattggc ttttactaat gtgtcgtaaa
     4321 aataaaatgt gttggaatta ttcgcattga tacataagct gtcgtatagt tccgacataa
     4381 ttaaattagg aaatgctttc gcagaaataa aagctgtatt tccttgagca acccagttta
     4441 aaagttgatg ctgttcttct tcgaataaat aatcctcttt accaacaaag aaatagattc
     4501 ctttatcgct cgattttagc gaatcttctt tccaagattt tgttaattcc ttgaatttga
     4561 aattcgggtt ataagattcg agaatttcac gaatcacata ggttccataa ggctgtttat
     4621 cttcgggctt gtatgtttcg taccaattga aataaggcga ggaacggttg cttgcataga
     4681 taaaaatacc aaccaacacc aatgctagaa acccgattag taatccgtta ggagattttt
     4741 tcattcgcgc ctcccttcct gattacgccc gatttcctgc ctgaattgca tgaatcgagg
     4801 ttctaatttg cgataggttt tttcgtcggg tggcgtgtgg ctgtaccaac ttttctcgaa
     4861 ccaacgagtt aaatcgcgga atggtcgaaa caacggtgtg tttcgtaatt cagctacata
     4921 atcggcattg gttttatctt ttttccagcg gatgaatttt tcgcgatgta gatcggcaag
     4981 cgtttgcaga taaagcaatc ttactgcaaa tgcataattc ccatttcgca aacattcgtc
     5041 gagtttttgt tgcagaattt gttcgggtga tgcttctttt tcaatatcta cttgcaacca
     5101 atcgggatct tcttttaagc gtggattggt ttgtttggct tgattgataa tgagcataat
     5161 caacacaacc aacacaatca gaatgacaat aattagaact acttgaccaa ttcctagaaa
     5221 agaagatttt ccactagtct caatttcttg tttaggtaat ttcttttcct ccttttttgg
     5281 ttgaggtggt tctttataat cgagtccttg aatcgccctt tggtaaagcc tttcatcgaa
     5341 ttgctttttg cccgtttttt cgtttttgta ttgacggctt aaactatcga ttgaaaatcc
     5401 agctggtctt ccatcaaaaa ttccttcctc atcatttata cttattgttt catcctcatc
     5461 atcataatca gcggtactat catcataagt gataaccgaa tcagctacta cagtatctga
     5521 ttgttgagca gttaacaact gacaaaacag caaacacaat cccaccaaaa tgctgtgaat
     5581 ctgtttcatt cgtttactaa tccagcttta cgtaaacgtt cgaacaaacc tggggcttca
     5641 cttatttccc gacaggaata aaacgaaaat gacatgcccg acagaaataa aggaatcacc
     5701 aaaaacaaag cagtagtctg aagaaagaca atggttccaa ccttaatcca gtaataggta
     5761 aagtcgccaa ctttgaaatt ccatgttaga actgaagtgt ccattaaagg taaaatcagg
     5821 tcggccaaga ggaaaaagaa aatggcaatt aatcctaaac ttaagaaagt cccataatga
     5881 cgcatgaggt tttcgaaagc atttttaaat attaatgagg tagctgcacc aaatgatagt
     5941 tcagaaactt gccatccagc aagccaaagc agcataaagg ggagaatcag ataaaatagc
     6001 aaatagaaaa atcctatatc aggaataaga ggtaatacta gaattgatag aattaaaacg
     6061 cttccaccaa atttggataa ttggaattta aaacctgttt tactgcgcgt ttcacccgga
     6121 gctaaaagca aggattcgct tctccagtaa tttccgaaaa ttgttgcagc taatgctcca
     6181 ccaataataa atagttcagc gaaactgtac ttgaaattaa atggtaatat ctcgggtatt
     6241 ttattgataa accaagtgtt gtaactgata ttcaatatct tatcctcggg caataaaagc
     6301 aataagctaa tggtatataa gagtgatatt cctaacaggt atttccaatt tttagaaagg
     6361 atgcgaatga acaaactaaa actatcaccg aaaataatgc tgctactttt cacctgatcc
     6421 atttcgggag caaacttggg tgtttgagga attttctgtt catcgagtcg gaatggtaca
     6481 ccagatcggg atttccggaa tggaaagatg acaaaatagg caacaataaa acctgcttgt
     6541 aacaagataa aaccgagtcg tatgaaattg tcgagttcgg tatagcgggt gatgaatcct
     6601 tcgattagtg cggcaaccag gataatggga gtaattcccg ccatgatttt tattccacta
     6661 cgtgccgaaa tacgtaatgc ttgcaaacgc gtaaaagtcc ccggaaaaac caagcctttt
     6721 cccatcacca aaccagcgcc accagcaatg atgatagccg aaatctccat cgtaccatgc
     6781 atccaaatgg ttagcgccga atcaaccaat aaaccacgac tgatgaaata atactggaaa
     6841 acgcccacca tgataccatt cgagacggtt gatccaatcg ttccaatagc gaatagaagt
     6901 ccgaagagga atgtccgaaa agctacaatc aagttgtttt gtgcgatgta caaaaacatg
     6961 ttgagcgcat ccttatcctt ataaaccgcc atgggatcgc ccgagctgat gttcgaattg
     7021 gtcatgctca cataatcggc tccgagaatc gtttgggcaa aattggtgtc gtaatgactc
     7081 gataacgcgc caatcaacat acaaaacaca aatagcacga acgaaaaaat gagttctttc
     7141 ctgctatggt agataacggc aggtaaatca tccgtccaga aataactgaa tcgatttttg
     7201 tgatttactg tgtttcgaag gagtgttcca aatacttttt gagtgagttg gttgaggtaa
     7261 actcgaacta cacgattgcc gtaatacgtt ctggcatagg ataaatcgtc gctcacctga
     7321 acaaataact cactcgcttt atccggatca cgtttcgatt cagaaagcat gcgctcgagc
     7381 tcaatccact tatctttatt ttgctctata aagcgcgttt ctttcatgca gggaaaatcc
     7441 agtatcttgg aagcggaaaa ataggaatta tactcttacc agaatgcgtc gtatcgaaat
     7501 cattacgcct caaaacgtac ctatacttta tcagttagca tcggtgcgcg aacgtatgtt
     7561 agcttttttg ctggatatat tgattttact gatcacgctc tatatcatct acctaatttg
     7621 cgcttcttct tttgatgaag atacgttaat gaccatcact ttcctttttc tgcttcccat
     7681 ttttatgttc tattcgttgg ggatggaagt gtttaataac ggtcaaacta tcggtaaaaa
     7741 agccatgcgc ataaaagtgg tgaaacttac cggtttagag ctttccctgt cggattacct
     7801 cttgcgttgg gcttttcgtt ggattgatat ctggggctct ttaggttcta ttgcagcttt
     7861 aaaagtaagc tcctccaccc gtggacaacg aatcggcgat ctactagccg acaccaccgt
     7921 tgttcgtaca aatgccgatt ttactgtatc acttcaggaa ttattggcca taaaaagtac
     7981 aaccgatcac gaaatagctt atccgggcat taagcgtatg aaggaagagg aaatgctact
     8041 ggtgaaaatg gtgttggatc aatacaaacg atttccgaat ccggcacatc atcgcgtaat
     8101 tgaacaaacg gcaaaaggaa tagcagaacg tatcgactta gaccacgtac ctaaagacaa
     8161 acaaggattc ctcattcggg ctttaaccga ttatgtgacc ttgacgcgtt cggcttagtg
     8221 agatttagaa tctcaaattc accccaacac ttggtaaaat cggcaactga ttaattcgtt
     8281 ggaatttcac ccggttgaag taaaatatgt tgtcgcgatt gtaggtattc gttacgccag
     8341 cagtaagttc tagggtagta ttttctccca aaacaactgt acgttttaag ttcacatcta
     8401 agcggtgata tgcaggtaaa cgaccttgat ttaacgctcc gtaagttgat cctaacgtac
     8461 cattagcgga agtataatcg ccattgatgt tgctgcttaa gttgatgttt tcgtagaaac
     8521 cttgtgtttg cgtgaaaggg aaacctgatc cataattcca acgtgcgctg aaagtccagt
     8581 taagtccttt cccaaattca taggaagcca ataagttcac attgtggcga cgatcccaaa
     8641 ccggactata atcgatcaat tcatcctcac gagtaacttt cattaaggaa taaacagccc
     8701 aaatatagaa gcgtttgtaa tcgtatttca aggtcatatc aatccctttg gaaatacctt
     8761 tttcgagaat gaaatctttc ttctgtacat ctggtttatc ctcgttttcg gcgttatcgt
     8821 cgtagagttt atttcggttg atattcgtta actgattaaa attgaagtaa taagcttcca
     8881 cattccactt aatgtgtttg gtaaggttgc tttcaaatcc gaaaataaca tgttgtgctt
     8941 tttgcagttt gctcgtacgt gtttcccctt tgaactcagt tggtgtattg tcgggtgcag
     9001 ttaagaatcc gtaaaataaa ttcaccacat cacgatcgga aactcctgca attaaattct
     9061 gactgtataa tccgcttgca aacttcaatc ggaaattgtc ggtaacattg tatttcaagc
     9121 ccaaacgtgg ctcgggagaa gcttctccta aagatgcgta ataatgaagt cggaaacttg
     9181 gttcagcaac tagttttcct accgtaaagt gatacttcgc gtaaagtgct aattcggtat
     9241 tgtttacgtc ttgttcaatc ttgcggttta gttcgttaaa gaaggtgtaa ttcgtattta
     9301 aaccaatcgc ctcaacgccc cataataaat cgtttttacc aaagaaatat ttgaagttca
     9361 aggctaaatt aaagcccttg atttcgctgc ttcgcgaagg caaagtttgc tgatctaggt
     9421 tgattttata atcgctgtag ctaaatactc cgtccatcac aaaattcgaa ttccccggaa
     9481 ccaaaacgaa gtttgctcct gcaccaaacg tattccaatg taagtcctgc aaactttggt
     9541 atttcacgcg gtcgctgaaa ttaaaaccag ttaggtttag cttactacca ttatcaccat
     9601 tgaacgacaa tttaccatac aaatcggtga agttgaacgg caatcccgct gtatctacat
     9661 acggataaaa cactttagaa ctctgtttca aataggaaga tcgtccgcta aggatgagga
     9721 agacgaacta gcctttccat ctttttggcg acttaatgga ccttcgagtt gaagcttaga
     9781 aacgaaagga cttgccgaca cttttccacc gaaacgtttt ttatcaccgt ctttggtggt
     9841 gattttcatg atagaagaaa cacgtccgcc attttcggca ctaaaacctc ctgtgtacac
     9901 atcagcattc cgaataacgt cagtatcaaa cacagagaaa agtccgattg agtggaatgg
     9961 attgtaaatt atcattccat ccagaagaac tttattttgg ataggcgaac ctccgcgaat
    10021 atagagttgt ccaccttgat ctcctgtaaa cgtaacacca ggaagaactt gcaggtattg
    10081 agcaatgtcg ggttcagctc caatggttgg aagcttattc atttcttttg gtgtgatggt
    10141 agttaaaccc acgccaatgc ggttttgctc ttcttctttt tcacctctta cttcaaactc
    10201 cttaatgtcg acagctttcg actgaacata gaggttctga gtaagaattt gtcccgcctt
    10261 aacattaaca tttacggatg ttttctcaaa tccaatggaa gaaaaaacga gtgtgtaact
    10321 tcctgctggc actttcgtga tggtatagaa accatttacg tccgtttgag ccacaaattg
    10381 agttccgtcg aggtggacat tggtgaaaat aaccggttcc ccgtttttct tattgtaaac
    10441 aactcctcgg attgttgctg tttgggcaag tagcaaagtc ccgacaatac tgaaaaagaa
    10501 ggcaagaaag taacgattca acatacgtgt attctggtga tgggcggcca aagatagaac
    10561 gattcctcgc atcccaacag aaacagttaa gggcttttat cggacgattt taaaaattaa
    10621 tagagaatgg atgtgggata ggagcaaagc ttccgatata aactaaacgt cataatgtaa
    10681 tttacactga atgatagagc atttctggtt tattcctttg gttcctgtga tttgataaac
    10741 caatcggtgt tctccagata ttctccttga ccaaaatccc ttcaatccaa attttaaggg
    10801 ttctagtttg cctattcctt tgaatggatc tttacgtata gcctgaatta gttctttaat
    10861 cttatttact ataaccggat catgttcgac ccaaaacgaa aattcttccc atgccaattc
    10921 ggtaaattca attgtcataa tcgaaacgaa ttaatcgatt ttataaggaa tcgtcttgga
    10981 cttttctaac tgcttaatcg acttctgtaa tcggttttga tttgcttggg ttgataataa
    11041 atgctctgtt tcttttaagg agttatattc ttgaatagac attattacaa tagcttcttc
    11101 ttctttcgct ctaggaacaa taattacatc catcgatttg gttacgtaat cgaagtactc
    11161 cttcattcct tttctcaaca aagagatcga tatcgctttc atagttcagc atttattctt
    11221 gcaaatgtac agaatgttgt acacaatagc gtacaaaatt aatgcacttc atcgattgat
    11281 tttagattaa aaaattgaat ggtaattagt tggaactatt catccccata aaccggcatt
    11341 cccttcaccg gaaagatttt ccccttcttc aaatcaatcc ttgctgcaag taaacgcatt
    11401 ccattggtaa ccactaaaaa ggggactttc atactcaaat tataccgcgc tgcttggtca
    11461 aacaccgatt gattcacttc tacctcagct gccttgcact caatcaacat ccagggattc
    11521 atctccgaat catacaccac aatatcggca cgcttcgata agccattcac cttcaacgaa
    11581 acctctacag ctatcttatt tttgggatac ttcaactcat ccaacaggta gtgtaaaaca
    11641 tgctgcctta cccattcttc gggtgttagt gctacatatt tgtttcgaaa agcgtcgaaa
    11701 atttgcgttc tttgtcctgc tacctcccgt gtgaggggcg tgatagctgg caacagaagt
    11761 acccgtaaac ctaaattagt aggacccatg aaaagcaaag aagaaatcgt caataattgg
    11821 ttgccccgat atactgggat gcagctcgaa gactttggca aatatatcct gctcaccaac
    11881 ttttccaact atgtggagaa atttgcagcc attaccggag ccaaagtagt aggtctcgac
    11941 cgtcccatgc cttgtgctac agcaaatgga ataaccatca ttaactttag catgggtagt
    12001 cccaatgctg ccattatcat ggatttgcta tcagccatca aacctaaagc atgcttgttt
    12061 ttggggaaat gtggcggatt gaaaaagaaa aataaagtgg gagacctgat ccttcctatc
    12121 gctgctattc gaggtgaagg aacatctaac gactactttc ctgccgaagt gcctgcctta
    12181 ccagcattta acttgcaaaa agcagtatca accaccattc gtaaccacaa caaagattat
    12241 tggacaggaa ccgtttttac taccaatcgt cgcgtttggg aacacgataa tgacttcaaa
    12301 aaatacctac agaaaattcg tgctatggcc atcgatatgg aaacggcgac cattttttct
    12361 gtcggatttt acaataaaat tccaactgga gcactacttt tagtgagcga tcaacccatg
    12421 gtagctggag gagtgaaaac cgaaaaaagc gactccgcgg ttacgaccaa ttatgttgat
    12481 tcacatattc aaattggaat cgattcactc aatgagttga tcaataacgg aaatacagta
    12541 aaacatttaa gattcgaata attgcatcaa gcctaatttc tcaggcattt tctcatggct
    12601 aaatcaagta cctcatccgt atcggtagaa tcggttttta aagagttgca ggcacgtaaa
    12661 ttcgctcctg tttatatgct ctgtggagag gaatcgtatt acatcgatca actttccaat
    12721 tacatcgaaa acaatgtgct taccgaggat gaacgcgagt ttaatcaaac ggttttgtat
    12781 ggtattgatg taacagccga tgatgtaatg gcaactgcca aacgatatcc gatgatgagc
    12841 gaatatcagg tggtgattat caaggaagca cagcaattaa gagatattga gaaattagtt
    12901 ccttacctga ataatccgca atcgagtact gttttagtta tttgctacaa aggaaaagag
    12961 ccggataaac gcaaggaatt ttataaatct ttgttgaaga acgatgctgt ttattttgtt
    13021 tcagagcgac cgaggcaaga gaaaatgcct gaatgggtta accgttattt tactaaaaag
    13081 ggttatcgca ttactcccaa tgccgctatt ttattgtcgg aatttgtggg gaatgaatta
    13141 gaaaaagtct gcaacgaagc cgataaatta tttctgaatt cacctgctgg ttccgaaatt
    13201 aatgagaaag acatcgagcg tcaagtgggc atttcacgtg aattcaatgt atttgaattt
    13261 caaaaagcat taggcactcg aaatgtgttt aaggcaaatc aaattgcact caatttagcc
    13321 ggacaaaaag ccacgccatt gccaatggtg atgggaagtt tgtttagttt tttcagcaag
    13381 ttattacatt accaatggct taaaagtaag ggcgaaacaa acatggcttc tgaaatggga
    13441 atttcacctt atgtgatgaa agattacgaa atggctgcac ggaattatcc gccatttaaa
    13501 ctgcttcgta tcatgagtca tttgcgcaat tatgacctca aaagcaaagg cctcaataac
    13561 gaaagtgcca acgaaaccga attgttaaaa gaactcacct ttaagattgt acactgatga
    13621 attattggct ggtgaaatcg gaaccggtaa aatacgcctg gtctaaattt gtaaaggaca
    13681 aaaatacagt ctgggacggc gttagaaact accaagcacg aaacaatctt caagcgatga
    13741 aactaggcga tctcgtcctg ttttatcata gcaacgaggg aatgaacgtg gttggaattg
    13801 ccaaagtggt gaaagaagcc tataacgatc ctacaaccga tgatttacgt tggttcgtcg
    13861 tagaattagc tccggtagaa gccttgaaaa aaccagttag ccttgagcaa atgaaatcgg
    13921 atgagaaatt ggcaaacatt ggattaattc gtcagtcgcg tttaagtgtg atgccattaa
    13981 ccaacatcga attcgaccga attttagaat tgggaagcta atcataccgc taattttatt
    14041 tagaattata aaaaattaac caatctggtt aagctatatg ggtgtaacca taaatcatcc
    14101 tactttaggc ttgctcaacc tttagcaata tgttcattta taaatacttt tctaaaagaa
    14161 acattaatcc gttcttttat ctagttccac ttgtatttct agtcgtttct tgttcaaatt
    14221 catctaatga atccaaacaa aatgcgacac atgttgcaga ggtagattcc tattctgttc
    14281 ctcaaaaaac gattttagcc acattacctg attcggctca ggtaaaaacg attcttttag
    14341 cagatcaaga aaaactcatc cacacaaaca ttcctcgcaa tggtgcttca gcaagagcag
    14401 ctgtgaacat ggatggaaaa gcgcagcaaa ttttagttga aagtccaatc acaaaaccac
    14461 tgagctatac caaaaatgga cagggacaaa tcaacctcga tcgaaatgga aagccattta
    14521 ttttaggaaa tggtggtaga gcatccttta agcaattcac ttcagagact ggattggcta
    14581 tggatgttgt agtatgtgct tttactgatg ccagtggaaa tatttggttt ggaactttgg
    14641 gcggtggtat ttcgaaatac gatggtaaat catttactaa ttattccact gcacatggtt
    14701 tacccaataa ccagatttat gggattactc aagataaatc gggacgtatg tggattgcta
    14761 ctttaggtgg cggaatatca tgcttcaatg ggaaaaattt caccacttac aaaaccgaac
    14821 aaggtttagc tagcgatttt gtaaactgta ttttggtcga caaaaatggc ttaatctgga
    14881 gtggaactcg tgatggaaaa gtatccagtt ttaatgggaa gaaattcacg aattataacc
    14941 ttgctaaact tcccggtgat gatgttataa atagtattta cgaagattta aatggaaatc
    15001 tatggtttgg agcagagatt tctgggctac atcgcttcga tggtaagcaa ttcaagaatt
    15061 atacaaccac tcaaggttta gctggaaatg ttgttcgttg cattaaaggt gattcaaaag
    15121 gaaatatctg gattggaact ttcgataatg gaatttcttg cattcctgct caacaaaata
    15181 atcaagctgg aaaagcaatt atatttaaaa attattcctc tcaagacgga ctttccgaaa
    15241 atgatgtttt ctccatgcac gtcgatcatc tcgatcgagt ctggtttggt accgatggag
    15301 gaggagtaaa ttgtttaaat cctgaatcga tagctgattc gagtaaagga cgattattta
    15361 cttgttataa ttccgaatta ggattggcta ataattctgt atttggaatt acagaagatt
    15421 taaacggaca tatttggtta tgtacatatg gtggtggagt tgttcgattt gatggcgaag
    15481 catttactgt tttctccgaa aaacagggct tagcggccaa taacatcatt tgtattaatc
    15541 aagataaaag cgggaattta tggttgggtt ccatagggaa aggtgcttcc tgtttcgatg
    15601 gaaaatcatt tacgaattac tctcctattc aaggtttggc aggtagtgta gtcacctgta
    15661 tgatgaatga ccgcaacggc aatatgtggc ttggtaccga taatggtggt gctgtattgt
    15721 atgatggaaa atcattcacc agctacacag ttgctcatgg attaagttct aattacatca
    15781 taagtcttta tcaagatcgt tcgggtgcta tttggattgg tacagtgggt ggcggagtgt
    15841 gtcgctttga tggaaattca tttacgagct attccacacc acaaggttta gcgggtgata
    15901 tggtgatgag catactcgaa gataaatcgg gta
//
LOCUS       DAOOQW010000038        15630 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_4592_length_15630_cov_5.594029, whole genome shotgun sequence.
ACCESSION   DAOOQW010000038 DAOOQW010000000
VERSION     DAOOQW010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 15630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15630
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4592_length_15630_cov_5.594029"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..951
                     /locus_tag="PK281_03990"
     CDS             <1..951
                     /locus_tag="PK281_03990"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY48536.1"
                     /translation="SFAKKVKFAVNMTGLIVNSTGVHVTGDFQAIAGFENGDWQSNTT
                     PLTQESADTNIYSIVVDIPAFAKYEYKFLNGDQFYDVEFVPVESRVGYNFNDNRWLYV
                     DSLANDTTFVGAILFGGNAPVNKKLIRFLVNMENETSVATNGVHISTSFSGNNPARNI
                     LYNFDSLIYEGILYADSNVTHSFRYYNGSLSSEAEVLPLDCSVNEERQITLVDHFVLP
                     VVCFASCSNCNSVGHETITSPSLSLFPNPAQSHVQITSSQSSISSYSIFDMAGQVVRS
                     QKLNEVKSCVINQEELPAGIYFVQSSLTDGSTRTLRLIWN"
     gene            976..3234
                     /locus_tag="PK281_03995"
     CDS             976..3234
                     /locus_tag="PK281_03995"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HOY48537.1"
                     /translation="MRIYLTLIAGLFHFCVQAQSNFYNISTIQKIEIYFSQTNWDYQL
                     DSAKAGSEGYIMADWVQVNDVQFDSVGVKYKGNSSYNANNTKNPLHIALDEFKSNAYQ
                     GITDIKLGNGFSDPSYIREVLSYQILGNYMDCPKANFAQVYINDVYYGVYSNAESINK
                     QFCADRFGTSSGTFIKCNPIVNPGPSNKCNLKFLTSDSSSYFNFYEMKSDFGWNKLVA
                     LCDSVTNTPASIDHVLDMDRVIWMLAFNNVLVNLDSYSGAFAQNYYLYQDNTNRFNPI
                     IWDLNMAFGGFPNLGSGNSSMGSLNLSAMQQVALNIHATDAYWPLIKDVQANPMFKRM
                     YLAHVKTIVSEMFAGNEYQTMATNLQAIVDTAIQSQTNPTYSYSQFQSAMTTSVMAGP
                     FTIPGISTLMSERVTYLQNTSDWNLVAPLINTVTASPSPAVLFETVHFSAAVAGSSIV
                     YLGYRFSENDRFTRVQMFDDGAHNDGAAGDNVFGLDLTMNSLFCQYYVYSENTDAGIF
                     SPQRAEHEFYTLLAETQEESDSPLVINEFMVSNTNSVTDNADEFEDWIEIYNSTNQAI
                     DLTGMFLTDNPLNLNKWDFPEGSSIPAGGYYIVWADEDSSQGINHCNFKLSAVGESLY
                     LLDENLAFVDSITWGPQPSDRSLARIPNGNGPFVLSTHSYNAFNPTANGFEVLSDQGE
                     LMVYPNPAKDILWVQQTQLKSETLVLMDVSGKIIRSQQFSGQTKLNTSDIAPGMYLLR
                     VGSHVKKILIQP"
     gene            complement(3309..5690)
                     /locus_tag="PK281_04000"
     CDS             complement(3309..5690)
                     /locus_tag="PK281_04000"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HOY48538.1"
                     /translation="MYVIKRDGRQESIKFDKITARIQKLCYGLEPVHVDPVSVAMKVI
                     QGIYPGVTTEALDNLAAETAASMTVKHPDYALLASRIAISNLHKTTKKSFSETMKDLY
                     FFVDPKTNQAAPLIADDVYEIILKNAELLDSTIIYDRDFGYDYFGFKTLEKSYLLKIN
                     NSVVERPQHMLMRVSVGIHKDDIENVIKTYDLMSERWFTHATPTLFNAGTPKPQMSSC
                     FLITMKDDSIDGIYDTLKQCAKISQSAGGIGLSIHNVRATGSYIRGTNGTSNGIVPML
                     RVFNDTARYVDQGGGKRKGSFAIYIEPWHADIIDFLNLKKNHGKEEVRARDLFYALWV
                     PDLFMKRVEEEGDWSLFCPNEAPGLSDCWGEKFEELYERYEAEGKARKTVKAREIWSS
                     ILEAQIETGTPYMLYKDACNRKSNQQNLGTIKSSNLCTEIIEYTDENEVAVCNLASIA
                     LPRFVIDGKFDHNKLFEVTYQITLNLNKIIDRNYYPVPEAERSNMRHRPIGIGVQGLA
                     DAFILLRYPFECEEAQKLNREIHETIYYAAMTASKDLAIKDGHYESYPGSPVSKGIFQ
                     FDMWDVTPSNRWEWDVLKEEVAKYGVRNSLLLAPMPTASTSQVLGNNECFEPYTSNIY
                     TRRVLSGEFIIVNKHLLRDLVDLGIWNEQLKDKIIGANGSIQGIVEIPDNIKELYKTV
                     WEIRQRTIIDMAADRGAYICQSQSLNIFMESANMAKLTSMHFYAWKKGLKTGMYYLRS
                     KAAADAIKFTVPSQAEPQKVAVVAEAQNVMVMDAQGEISCSLDNPEGCEACGS"
     gene            complement(5731..6711)
                     /locus_tag="PK281_04005"
     CDS             complement(5731..6711)
                     /locus_tag="PK281_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006989221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase small
                     subunit"
                     /protein_id="HOY48539.1"
                     /translation="MKNVIADEPILQENKDRFVLFPIKHTDIWQMYKQAEASFWTAEE
                     IDLHADLTDWENKLTPDEKHFIKHVLAFFAASDGIVNENLAINFMKEVQYPEARCFYG
                     YQIMCENIHSETYSLLIDTYINDTAEKNRLFHAVENVPAVGRKADWALRWIENGTFAE
                     RLVAFASVEGIFFSGSFCSIFWLKKRGLMPGLSFSNELISRDEGLHCDFACLIYKQLR
                     HQLSEERVKEIISNAVEIEKDFVSDAIPVSLIGMNADLMCQYIEFVADRLLIALGCTK
                     IYNASNPFDFMEMISLQGKTNFFEKKVGEYQKAGVMGKQIDNTIRFDEDF"
     gene            complement(7154..7732)
                     /locus_tag="PK281_04010"
     CDS             complement(7154..7732)
                     /locus_tag="PK281_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3109 family protein"
                     /protein_id="HOY48540.1"
                     /translation="MLIVGDILVSEDLLEKKFVCDLQACKGTCCVQGDAGAPLGEEEP
                     GILEEEYEHFQSYIRPEGREVVKRDGVFTIDSDGDVVTPLIDGKECAYTVFDEQGIAG
                     CGIEQAWLAGKTKFRKPISCHLYPVRVKKLVDIDALNYHTWDVCKPACECGSKLQVPV
                     YRFLKEALTRAYGEEWYSQLELIAKEWEKGSK"
     gene            7795..8304
                     /locus_tag="PK281_04015"
     CDS             7795..8304
                     /locus_tag="PK281_04015"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOY48541.1"
                     /translation="MAAYDNLEFEDMLFEADELIRNNKIADAVSLLEGVIAQAPDFGK
                     AYNHLGWVYETKLKDYTRAENMYRQCLAFNPDYSPVYLNLSIVLSSLGKYDDLEDLLN
                     KAILVPGTDKAAIYNEFGILFELKQDYNKAIENFKTAIRFTLSDVNVDTYMKSIDRCR
                     KKRELLSSI"
     gene            8335..9792
                     /locus_tag="PK281_04020"
     CDS             8335..9792
                     /locus_tag="PK281_04020"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013750436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HOY48542.1"
                     /translation="MSQPDDLFKKVSAHAKEYGFVFQSSEIYDGLGAVYDYGQNGVEM
                     KNNIKEYWWKSMVQMNENIVGIDSAIFMHPKVWKASGHVDGFSDPMIDNKDSKKRYRA
                     DQLLEEQIEKYEKKGKSDKAAELEAAMNKALNDNDLDAMLNLIVEHEIVCPISGTRNW
                     TDVRQFNLMFSTQMGSLAEDADAVYLRPETAQGIFVNFLNVQKSGRMKIPFGIAQIGK
                     AFRNEIIARQFIMRMREFEQMEMQFFVRPGTEMEWFAKWKETRLKWHLALGTPAAKYR
                     YHDHLKLAHYANAATDIEFEFPFGFKEVEGIHSRTDFDLKQHQEFSGKKMQYFDPEIN
                     QNYIPYVIETSIGLDRMFLLTLCNAYKEEEVGEGDKKEIRTVLALPPQLAPFKVAILP
                     LTKKDGLPEKAREILDMLKPSYRCHYEEKDAIGKRYRRHDAIGTPFCVTVDHESLETN
                     TVTIRHRDSMVQERVSISDLTSILEKHVSMRSLLS"
     gene            10082..11494
                     /locus_tag="PK281_04025"
     CDS             10082..11494
                     /locus_tag="PK281_04025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOY48543.1"
                     /translation="MSELEKLSLVASRTSNIVIITDSNRRIHWVNEGFTRITGFNMEE
                     SIGKKPSELLFGPETDLVKAEEISARLNKGETVKEEQINYTKDGRKYWVQLDITPVLN
                     EKGELNAFVCIGDDISIRKEKEQKLRRNWREYDEIYEQAVMPMCFTNTQGIFVRVNQA
                     YCDLFECKREDLLGKDFIQTHFSKMPDSGQMELRLEADRIVKQRDTTKKEMRFSTFSG
                     KPLVIEVIRKMVEVNGHPVVSIYLVDTTEKRDFQLRILEQNKRLKEFAFLTSHKLRQP
                     LANILGLIELVKSESHSRQDVTITLETLRMLTSQLDGVVHEMGQALAELDVEVEKSLF
                     FNEQEDQRIHDVWIVDDDQVITYITQRLMKNADPTVKVTEFLSAKMALEKLRIDQAQP
                     DILLLDINMPGITGWEFLDELRSMHRFVNVYMYSSSIDPEDVKKARSYPMVRDFLSKP
                     IDMETVRNLLSIPIIRSQVS"
     gene            11546..12502
                     /locus_tag="PK281_04030"
     CDS             11546..12502
                     /locus_tag="PK281_04030"
                     /inference="COORDINATES: protein motif:HMM:NF016292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOY48544.1"
                     /translation="MNRILYVAFLSLLISFSSCNQSDARKGMTTIAFLDAFEDATIAE
                     AKKGFFRALRDSGYIPEKNLQVIYRNAQGDIPALTQSVDYFISQNVSLIATNTTISTI
                     SAVQKTNQIPVCMMVSPSPELAGLRNAKGQDPENLFGVYETLEYIDTAITLIHDVFPQ
                     AKRVGTLINQSEPQSVDALERIRKDAASLGLEVISLPANSSAETQLVMERLIDQKIDV
                     FFALPDNTIFSSFETVVSSCDKANVPIITSEAGLVSRGAVAGFGANMYDWGYASGQEA
                     AQFLTTRKLPAIKKLNKRVRMYNPSQAKRFNFQPGPEFKAVN"
     gene            12502..13374
                     /locus_tag="PK281_04035"
     CDS             12502..13374
                     /locus_tag="PK281_04035"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48545.1"
                     /translation="MQFYLTAISLGLALSALGWGIFLTLKIFNIPDITTDGSFTLGAA
                     ITAVQLTNGTPWYLVLFCSMLGGGLAGMLTGVIHTRLKVNALLSGILVMTALYSVNLM
                     VMGRSNIPLLQVNHLFIAFQPTGASYIDQAIILLAIMSIVGLCLIFMLKTDFGLAMRA
                     TGNSESMVNAMGVNTNRMKIIGLALANSLTAFSGSIVAQLQQFTDINMGIGIVIFGLG
                     SVMIGETLMDVFKRQGIAWRIIGVVIGCLIFRLVIAFTLASGADPNLLKLITAGVVLL
                     FVSIPRFKGKTAFR"
     gene            13371..14120
                     /locus_tag="PK281_04040"
     CDS             13371..14120
                     /locus_tag="PK281_04040"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOY48546.1"
                     /translation="MIEILKLNKTFHLGSERQVIAVQNLDISFENGSFNLLIGSNGSG
                     KSTLLNLIAGTILPDSGTISINGKPIQSIPSYKRSTSIARIFQDPTAGTAPDLSLLDN
                     FRIASLRTQKKGLRIGVDAAFRKVVAAHVSILDMGLENRLNQPMGSFSGGQRQALTLL
                     MATFDKLDVLLLDEPTAALDPKSAVRILELTQRIVAEKKIIAIMVTHELRQCIQVGTR
                     IIQLSEGQIIRDVAGNTKTQLRLEELANWFA"
     gene            14309..>15630
                     /gene="dxs"
                     /locus_tag="PK281_04045"
     CDS             14309..>15630
                     /gene="dxs"
                     /locus_tag="PK281_04045"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HOY48547.1"
                     /translation="MNIIPGELLAQIDSPADLRRFKEDDLVQICNELRQYIIDIVSVK
                     GGHFGASLGTVELSVALHYAFNTPYDQLVWDVGHQAYGHKMLTGRREVFHTNRVYGGI
                     SGFPKRKESEYDTFGVGHSSTSISAALGMAIASHLKDEKSRQHIAVIGDGALTGGMAF
                     EALNHAGASNNNMLVILNDNCMSIDPNVGALKEYLTDITTSHTYNRVKDEVWNLLGKI
                     SKFGPNAQAIVQKIENAIKSAVLQQSNLFESLNFRYFGPVDGHDVNRLVQVMNDLKDI
                     PGPKLLHVLTVKGKGYKLAEEDQTLWHAPGLFNKDTGEIIKSPKKGPEPPKYQDVFGH
                     TIVEIAKEDTRVVGITPAMPSGCSLNIMMKEIPDRAFDVGICEQHAVTFSAGLATQGM
                     IPFCNIYSSFMQRAYDQVIHDVAIQNLHVVFCLDRGGVAGADGPTHHGA"
BASE COUNT         4611 a         3376 c         2994 g         4649 t
ORIGIN      
        1 tcatttgcga aaaaagtaaa attcgctgtc aacatgactg gcttaatcgt aaactctact
       61 ggagttcatg ttaccggcga ttttcaagcc attgctggat tcgaaaatgg tgattggcaa
      121 tccaacacaa ctccgcttac tcaagagtca gcagatacca acatatatag tattgtagtc
      181 gatattcccg cttttgctaa atacgaatac aaattcctaa atggggatca attttatgat
      241 gtggaatttg ttccagttga atcgcgtgtt ggatacaatt tcaatgataa tcgctggctt
      301 tatgtcgatt ctctagctaa cgataccact ttcgttggtg ctattctttt cggtggaaat
      361 gctcctgtca acaagaagct cattcggttt ttagtgaata tggagaatga aacttccgta
      421 gcaacgaatg gtgtccatat ttctacaagc ttctcaggaa acaatccggc tagaaatatc
      481 ctgtacaact tcgattcact catctacgag ggaattcttt atgctgattc taatgtaact
      541 cactcatttc gttactataa tggttctctc tccagcgaag cagaagtcct tccgcttgat
      601 tgctctgtga atgaagagcg tcaaattact ctcgttgacc atttcgtgct tcccgtagtt
      661 tgttttgcca gctgctctaa ttgtaattca gttggtcatg aaactatcac ttcaccttca
      721 ctaagtctat ttcctaatcc agcacaatct catgttcaaa taacttcttc ccaatcttcg
      781 atttcatcct attctatctt cgacatggcc ggtcaagtgg ttcgtagtca aaagctcaac
      841 gaagtgaaat cctgtgttat caatcaagaa gaattgcctg caggaatcta ttttgttcag
      901 tcctctttga ctgatggatc tacacgcact ttacgcctta tttggaatta attcattcct
      961 actaaacaat caagtatgag aatttatttg accctaattg ctggactctt tcacttctgt
     1021 gttcaagctc agtctaattt ttacaacata tcaacgatcc agaaaatcga aatttatttc
     1081 agtcaaacca attgggatta ccaactcgat tctgccaaag caggaagtga agggtatatt
     1141 atggccgatt gggttcaagt aaatgatgtt caattcgaca gtgttggagt gaaatacaaa
     1201 ggcaatagtt cctacaacgc aaacaacact aaaaacccgc ttcacattgc gctagatgaa
     1261 ttcaaatcca atgcatatca gggcattacc gacatcaaac ttggaaacgg attctcagac
     1321 ccatcctaca ttcgagaagt actatcctac cagattcttg gaaattatat ggattgtcct
     1381 aaagcaaact ttgctcaagt ctatatcaat gatgtgtatt atggtgttta ctcaaatgct
     1441 gaatcaatca acaaacagtt ttgcgccgat cgttttggaa catccagtgg aacattcatt
     1501 aagtgcaacc caatcgttaa cccagggcca agtaataagt gtaatctcaa gttcttaact
     1561 tccgattctt cctcatactt caacttctat gaaatgaagt cggattttgg atggaataaa
     1621 ttggtcgcac tttgtgatag tgttacgaat actcctgcta gcatcgatca tgttttagac
     1681 atggatcgag ttatttggat gcttgctttt aacaatgtgt tagtcaatct cgattcttat
     1741 tctggagcat ttgcccaaaa ctactacctc tatcaagaca acaccaatcg ctttaaccca
     1801 atcatctggg atttgaatat ggcatttggt ggttttccaa atctaggaag tggaaactca
     1861 tccatgggaa gtttaaatct ttccgccatg caacaagtgg ccttgaatat tcacgcaact
     1921 gatgcgtatt ggcctttaat caaggatgtt caagcgaatc ccatgtttaa acgtatgtat
     1981 ctggctcacg tgaaaacgat tgtttccgaa atgtttgctg ggaatgaata ccaaacgatg
     2041 gcaaccaatc tacaagctat agtagatacc gccattcagt ctcaaacgaa tccaacttat
     2101 tcatatagtc agtttcaaag tgcaatgact acaagtgtta tggctggtcc attcacgata
     2161 cccggcattt caacacttat gtctgaacga gttacttatt tgcaaaatac aagtgattgg
     2221 aatttagtgg caccattaat caatactgta acggcaagtc catctcctgc agtacttttc
     2281 gaaacagtac atttttctgc tgcagtggct gggagttcga tcgtttatct cggttatcgt
     2341 tttagtgaaa atgatcgatt tacccgagta caaatgttcg acgatggtgc tcacaacgac
     2401 ggagccgctg gtgataatgt ttttggttta gacttaacta tgaactccct gttttgccag
     2461 tattatgtgt attccgaaaa tactgatgcc ggtatttttt ccccgcaacg tgccgaacac
     2521 gagttttata cactcttagc agaaacacaa gaagagtctg atagtccgct agtaatcaat
     2581 gaattcatgg tgagtaacac caattctgtt actgataatg ctgatgaatt tgaagattgg
     2641 attgaaatct ataactcaac caatcaagcc atcgatttaa caggaatgtt tcttacagac
     2701 aatcctctga acctcaataa atgggatttc cctgaaggct caagcattcc ggctggtggt
     2761 tattacattg tttgggcgga tgaagattct agtcagggaa ttaatcactg taactttaaa
     2821 ctatcggcag taggtgaatc gctttattta ttggatgaaa acctcgcttt tgtcgactca
     2881 ataacttggg gaccacaacc ttcggatcgc agtttggctc gtattccaaa tggtaacggt
     2941 ccattcgtgt taagcacaca ttcatataat gcatttaatc caacagcaaa cggatttgaa
     3001 gtattgtctg atcaaggaga actcatggtt tatcccaatc cagcaaagga tattctttgg
     3061 gttcaacaaa cacaactgaa atccgaaacg cttgtattaa tggatgtttc aggaaagata
     3121 attcgttctc aacaattttc tggacaaaca aaattaaaca catctgatat tgctcctggg
     3181 atgtatttat tacgtgttgg ttctcatgta aaaaagatac tcattcaacc ataaaaacga
     3241 caaagtgcca aataaaaagc ccctgtccaa actggaacag gggctttttt acaatcaact
     3301 atttttactt agctgccaca agcttcgcaa ccttctggat tatctaggga gcaagaaatc
     3361 tctccttgag catccatcac cattacattt tgtgcttcag ctactacagc aactttttgt
     3421 ggctcagctt gcgatggaac ggtaaattta atcgcatcag ctgcagcttt cgaacgcaag
     3481 taatacatac ctgttttcaa gcctttcttc caagcataaa agtgcataga agttaactta
     3541 gccatattcg ccgattccat gaagatatta agcgactgcg actgacagat atatgcacca
     3601 cgatcggctg ccatatcaat aatagtacgt tgacgaattt cccaaaccgt tttgtatagc
     3661 tctttaatgt tatcaggaat ttctacaatg ccttggatag aaccgtttgc accaataatc
     3721 ttgtctttca actgttcatt ccagataccc aaatcaacca agtcgcgaag caagtgcttg
     3781 tttacgatga tgaattcacc agaaagaaca cgacgtgtgt agatattgct agtatatggc
     3841 tcgaagcatt cgttattacc taaaacttgt gaagtagaag ctgttggcat tggagctagc
     3901 aataaggagt tgcgaacgcc atatttagct acttcttctt tcaacacatc ccattcccaa
     3961 cgattcgaag gagttacatc ccacatatca aattggaata tgcctttcga aacaggtgag
     4021 ccaggatagc tttcgtaatg accgtcttta attgcaaggt cttttgaagc cgtcattgct
     4081 gcatagtaaa ttgtttcgtg gatttcacga ttcaacttct gtgcttcctc acattcaaat
     4141 ggatagcgaa gcaaaatgaa tgcatcagcc aaaccttgaa caccaattcc tatcggacga
     4201 tggcgcatat tagaacgctc agcctctggt actggatagt aatttctgtc aatgatcttg
     4261 ttgaggttca gcgtaatctg ataagtaacc tcgaacaatt tattgtgatc aaattttccg
     4321 tcaataacga aacgcggtaa agcaatcgat gctagattac aaacagctac ctcattttcg
     4381 tcggtgtact cgataatctc tgtacaaagg ttcgatgatt taatcgtacc caagttttgt
     4441 tgattgctct tacggttaca tgcgtctttg taaagcatgt aaggagttcc tgtttcaatt
     4501 tgtgcttcta aaatagaaga ccaaatttca cgtgctttta ctgtcttacg tgcttttcct
     4561 tcggcttcgt aacgctcata aagttcctcg aacttctcac cccaacaatc tgaaagaccc
     4621 ggagcttcgt tcggacagaa taaagaccaa tcgccttctt cttccacacg tttcatgaaa
     4681 agatccggta cccaaagtgc gtaaaataaa tcgcgggcac gaacttcttc tttaccatgg
     4741 ttctttttca gattcaagaa atcgataata tccgcatgcc atggctcaat gtagatggca
     4801 aatgaacctt tacgttttcc tcctccttga tctacataac gagctgtatc gttgaataca
     4861 cgaagcatag gaacgattcc gttagatgtt ccattggttc cgcggatgta cgatcctgta
     4921 gcacgaacgt tatgaatgct taagccaatt ccaccggcac tttgactgat tttagcacac
     4981 tgcttaagtg tgtcgtaaat cccgtcaata ctatcatcct tcatagtaat gaggaagcaa
     5041 ctcgacattt gtggctttgg tgtacctgcg ttgaataaag ttggagtagc gtgtgtaaac
     5101 cagcgctccg acattaaatc ataggtcttg attacgttct cgatatcgtc tttatggata
     5161 ccaaccgaaa cacgcatcaa catatgttga gggcgttcaa caaccgaatt gttgattttt
     5221 aggaggtagg atttctccaa tgttttaaac ccgaaataat cgtatccaaa atcccggtcg
     5281 taaatgattg ttgaatcaag tagttcggca ttttttaaga tgatttcata cacatcatct
     5341 gcaatcaaag gtgcagcctg attggttttt ggatccacga agaaatacaa atccttcatc
     5401 gtttctgaga acgatttctt agtggttttg tgcaggttgg aaatagcaat acgtgatgcc
     5461 aaaagtgcat aatcaggatg ctttacagtc atcgaagctg ctgtttcagc ggctagatta
     5521 tcgagagctt ctgttgttac gccagggtaa atcccctgaa ttactttcat ggcaacactt
     5581 accggatcca cgtgtactgg ctctaatccg taacagagtt tctgtatacg agccgtgatt
     5641 ttgtcaaact taatggactc ctgtcggccg tcccgcttga ttacgtacat ggttagttag
     5701 ttaggtgttt ttaaaaaatg tctttttcaa tcaaaaatct tcgtcgaaac gaatggtgtt
     5761 gtcgatctgt ttgcccatta ctccggcttt ctgatactcg cccactttct tttcaaagaa
     5821 gttggttttt ccctgtagcg atatcatttc cataaaatca aatggattgc tcgcattgta
     5881 aattttagtg caacccaacg caattaataa tcgatctgct acaaactcaa tgtattgaca
     5941 catcaaatcc gcattcattc caataagaga aactggaatc gcatcgctca cgaaatcttt
     6001 ttctatttca acggcatttg atataatttc ttttacgcgc tcctcactta attgatgacg
     6061 tagttgtttg taaatcaaac aagcaaaatc acaatgtaat ccctcatcac gagaaattag
     6121 ttcatttgaa aagctcaagc ccggcattaa tccacgtttc ttcaaccaga aaatggaaca
     6181 aaatgagccc gagaaaaaga ttccttctac agatgcaaac gctaccaaac gttccgcaaa
     6241 tgttccattc tcaatccaac gtaatgccca atctgcctta cgtcccacag ctggaacatt
     6301 ctcaaccgca tgaaataacc tgtttttctc cgctgtgtcg ttaatgtagg tatcaatcaa
     6361 taatgagtat gtctctgagt ggatgttctc acacatgatt tgatatccat aaaaacaacg
     6421 agcttcagga tattgaactt ctttcatgaa gttaatcgcc aaattctcat tcacaatccc
     6481 atcactggct gcgaaaaatg ccaacacatg cttgataaag tgcttctcat ctggtgttaa
     6541 cttgttctcc caatcagtaa ggtcagcatg taaatcaatc tcctctgctg tccaaaaact
     6601 agcttcagct tgtttgtaca tctgccagat gtcggtatgc ttgatcggaa acaatacaaa
     6661 acgatccttg ttttcctgca atatcggttc gtctgctatt acgtttttca ttaggtcgac
     6721 ttcactcgtt attgatgtag gtaaaacgtt gctcattttg tttggtattg ataatcagtt
     6781 agttgcgttt tgtctgttgg cagtcgataa tcttaaaaag ctaagtggct tttaaacagg
     6841 gagatatcgc gaaaccttta ggaaaaaact tacttcacaa aaatgggtcg gaagggggct
     6901 tctcacaact cgtttttgtt aacatttgac tgcacttttt aacatttcgg ctggagcctt
     6961 aggagccatt gattttcagt acaaataagc atgggcgagc acttgggaat actacctctt
     7021 tagggagtta taaacacgac aaacgcttgt tagtttgaca gttaatccag agttaacata
     7081 gtccactcaa acaattctgt caagcacatt tgcagagatg aaacgctttg attatacgac
     7141 ttagtatcta cccctattta ctaccctttt cccactcctt ggcaattaac tctagctgag
     7201 aataccactc ttctccataa gctcttgtta atgcttcctt taagaatcga taaactggaa
     7261 cttgtagttt cgagccacat tcacatgctg gtttgcaaac atcccaagta tgataattca
     7321 atgcatctat atcgactagc ttctttacgc gtactggata taaatgacac gatatcggtt
     7381 ttcgaaattt tgtctttcct gctaaccatg cttgctcaat tccacatccc gctattcctt
     7441 gctcatcaaa aaccgtataa gcacattctt tcccatcaat aaggggtgtt actacatccc
     7501 catccgaatc aatagtaaaa acaccatctc gctttacaac ttctcttcct tcaggccgga
     7561 tatacgattg aaaatgctca tattcttcct ccaaaatccc cggttcctct tctcccaaag
     7621 gtgctcccgc atctccttga acacaacaag tgcccttaca agcttgaagg tcacatacaa
     7681 acttcttttc caataggtct tccgagacca gaatatctcc aacaatcagc atgggacaaa
     7741 ggtacacacc tcatcctcaa attccgtagg tttgcatccg caattaaaat actcatggca
     7801 gcatacgata atctggagtt cgaagacatg ctttttgaag ctgatgaact catccggaat
     7861 aacaaaattg ctgatgccgt ttctcttcta gaaggcgtca tcgctcaagc tcccgatttt
     7921 ggaaaagcat ataaccacct cggttgggtg tatgaaacca aattgaaaga ttatacccgc
     7981 gccgaaaaca tgtatcgtca atgtctggca tttaatcccg attattctcc tgtttacctt
     8041 aatctaagca tcgtcctttc ttcccttgga aaatacgacg atcttgaaga tttactcaat
     8101 aaagcaatcc ttgtcccagg taccgataaa gctgccatct acaacgaatt tggaatcttg
     8161 ttcgaactaa aacaagacta caacaaagcc atcgaaaact tcaaaactgc tattcgtttc
     8221 actttaagtg atgtgaatgt cgacacatat atgaagtcaa tcgaccggtg tcgcaaaaaa
     8281 cgcgaacttc tttcttctat ctgatttttc cttcatctta aataattcta cctcatgtct
     8341 caaccggacg atctctttaa aaaagtctcc gcccacgcta aagaatacgg tttcgtattc
     8401 caatcttccg aaatctatga tggactcggc gccgtttacg attacggtca gaacggtgtt
     8461 gaaatgaaaa acaacatcaa agaatattgg tggaaatcaa tggtgcagat gaacgaaaat
     8521 atcgtgggta tcgactccgc tatttttatg catcctaaag tgtggaaagc ttccggtcac
     8581 gtggatggtt ttagcgatcc gatgatcgat aataaagact ccaaaaaacg ctatcgtgcc
     8641 gatcaactcc tcgaagaaca aattgagaag tacgaaaaga aagggaaaag cgacaaagct
     8701 gccgaattgg aagctgccat gaataaggca ttgaatgata acgatttgga tgccatgctc
     8761 aatctgattg tggaacatga aatcgtttgc ccaattagcg gaacacgcaa ctggaccgat
     8821 gttcgtcagt tcaatctcat gtttagcact caaatgggtt ctttggccga agatgcagat
     8881 gctgtttact tacgccccga aacggctcaa gggattttcg tgaacttcct caacgttcag
     8941 aaaagtggac gaatgaaaat tcctttcggt attgctcaaa ttggtaaagc tttccgaaac
     9001 gaaattattg ctcgccaatt cattatgcgc atgcgcgaat tcgaacaaat ggaaatgcag
     9061 tttttcgttc gccctggcac cgaaatggaa tggtttgcca aatggaaaga aacacgtttg
     9121 aaatggcatt tagcgcttgg cacaccagca gctaaatacc gttaccacga tcaccttaaa
     9181 ctggcacatt acgcaaatgc tgcaaccgat atcgaatttg aattcccttt tggattcaaa
     9241 gaagtagaag gcatccattc acgcaccgat tttgatctta aacaacatca agaattctcc
     9301 ggtaagaaga tgcagtactt cgaccccgaa atcaatcaaa actatatccc ttatgtaatc
     9361 gaaacttcta ttggtctcga tcgcatgttc ttacttacac tttgtaacgc atacaaagaa
     9421 gaggaagttg gagaagggga taaaaaggaa attcgtactg tattagccct tcctcctcaa
     9481 ttagctcctt ttaaagtagc tattcttcct cttaccaaga aagatggact tcctgagaaa
     9541 gctcgagaaa tattagatat gctcaagcct tcttaccgct gccattacga agagaaagat
     9601 gcaatcggaa aacgttaccg ccgtcacgat gctattggta ctcctttctg tgtaactgta
     9661 gatcatgaaa gccttgaaac aaatacagtt accattcgcc accgcgattc aatggtacaa
     9721 gaacgggttt ctatttctga cctcacatcc attcttgaga aacatgtatc gatgcgttca
     9781 ttattaagct aatccgctaa aaattagttt tttgatgtct tgggtattgc tacctaagat
     9841 gaaagtttat aatcggttcc ggtacttttt ttctaataag agaaaacgtg ctggaacctt
     9901 ttgttttagg gctatgggac aagcttgtgg acagttttag ctattccaag tacaaatact
     9961 acctctcgtt tttatatgta aagaataaca agaattagat gcatttatga ctaattaagc
    10021 cgcaacctac acatgcgttt aaaggtaaac tctacctgaa aagcgagaga ttatgcacgt
    10081 attgtccgaa ctggaaaagt tatcgttggt tgcaagcaga actagtaaca tagttattat
    10141 taccgactcc aatcgtcgta ttcattgggt taatgaaggt tttacccgta ttactgggtt
    10201 taatatggaa gagtctattg gtaaaaagcc ttcagaactc ctctttggtc ctgaaaccga
    10261 tttggtaaaa gccgaagaaa tatccgctcg tttgaataaa ggagaaacgg ttaaagaaga
    10321 gcaaattaac tatacaaaag acggtcgaaa atactgggta cagctcgata tcacgcccgt
    10381 cttgaacgaa aaaggagaac taaatgcttt cgtttgcatt ggggatgata ttagcattcg
    10441 aaaagaaaaa gaacaaaagc ttcgtcgaaa ttggagggag tacgatgaga tttatgagca
    10501 ggctgttatg ccaatgtgct tcaccaatac tcaaggaata ttcgttcgag taaatcaagc
    10561 ctattgtgat ttgtttgaat gcaaacgcga agatttgctt ggtaaagatt tcattcaaac
    10621 tcatttctct aaaatgcccg attcagggca gatggaatta cgcctcgaag cagatcgcat
    10681 tgttaaacag cgcgatacca ctaaaaagga aatgcgtttc agcaccttta gtggtaaacc
    10741 tctcgtgatc gaagtaatcc gcaaaatggt agaagttaat gggcatcctg ttgtttctat
    10801 ttacctagtc gatactaccg aaaaacgaga ctttcaactc agaattctcg aacaaaacaa
    10861 acgacttaaa gaattcgctt tcctcacttc acacaaactt cgtcaaccac ttgccaatat
    10921 tcttggtctc atcgaattgg tgaaaagtga atcccattca agacaagacg taacaatcac
    10981 actcgaaaca cttcgaatgc ttaccagtca actcgatggc gtagttcatg aaatgggaca
    11041 agcgctggcg gaactggatg tcgaagtaga aaaaagtctc ttctttaacg aacaggaaga
    11101 tcaacgcatt catgatgtat ggattgtaga tgatgatcag gtgataacct acatcaccca
    11161 acgtctaatg aagaatgctg atccgacagt gaaagtaacc gaattcctaa gtgctaaaat
    11221 ggcactcgag aaattacgta tcgatcaagc ccaacccgat attcttctgc tcgacatcaa
    11281 catgccaggt attaccggat gggaattcct cgatgaatta cgctctatgc atcgatttgt
    11341 caatgtttat atgtactcat cttcaatcga ccccgaagac gtgaaaaagg ctcgtagtta
    11401 tcctatggtt cgcgattttc tatctaagcc gattgatatg gaaacagttc ggaatctctt
    11461 atcaattccg attatccgct cacaggtttc ctgattctaa ctttcacttt acaacaaatc
    11521 aagtaggatt cggtttcttt gtttcatgaa ccgaattctt tacgttgcct ttctgtctct
    11581 tctcatttcg ttttcatcct gcaatcaatc agatgcaaga aaagggatga ccaccattgc
    11641 ttttcttgat gctttcgaag acgcaactat agctgaagcg aaaaaaggct tctttagagc
    11701 cttacgtgat agtggctaca ttcctgaaaa aaaccttcag gtaatctacc gaaatgccca
    11761 aggtgatatt cctgctttaa ctcaatcggt cgattatttc atctctcaga atgttagcct
    11821 tatagctaca aacactacaa tctcaacgat ttctgccgtt caaaaaacaa atcaaatccc
    11881 tgtttgtatg atggttagcc ctagtcccga actagccgga ttgagaaatg caaaaggtca
    11941 agatcccgag aatctatttg gggtttatga aaccctcgaa tacatcgata ctgcaatcac
    12001 actcattcac gatgtttttc ctcaagcgaa acgtgttgga acgctcatca atcaaagtga
    12061 acctcaaagt gttgatgcat tagagagaat tcggaaagat gccgcctctc ttggacttga
    12121 agttatttca ttacccgcaa atagttctgc cgaaactcaa ttggtgatgg aaaggttgat
    12181 tgatcaaaaa atcgacgtgt tttttgcgct ccccgataat acaatattct cttctttcga
    12241 aacggttgta agctcatgcg ataaagccaa tgtgcccatc attacatccg aagctggact
    12301 agtaagtcgt ggcgcagtag ctggttttgg tgcaaatatg tacgattggg gatatgcatc
    12361 cggacaagaa gccgctcaat tcttaacgac aagaaaactt ccggcaatca agaaattgaa
    12421 caaacgtgtc cgcatgtata atccttccca agccaaacgg tttaattttc aaccaggacc
    12481 agaattcaaa gcagttaact gatgcagttt tatttaacag ccatcagctt aggtcttgct
    12541 ctttccgctt taggctgggg tatttttcta acgctcaaaa ttttcaatat tcccgatatc
    12601 actactgatg ggagctttac acttggcgcg gctattacag ctgttcaact aaccaacgga
    12661 actccttggt atttggttct attttgttcc atgcttggag gcggactcgc aggaatgctt
    12721 actggagtta tacatactcg ccttaaagtg aatgcgctgc tttcaggaat tcttgtaatg
    12781 accgcacttt attcggttaa tctgatggtc atggggcggt caaacatacc gcttttgcag
    12841 gtgaatcatt tatttatcgc cttccaacct accggcgctt cctacatcga ccaagcaatc
    12901 attttgttgg caatcatgag cattgttgga ttgtgcttaa ttttcatgct aaaaaccgac
    12961 tttggtcttg ccatgcgcgc aactggaaat tccgaatcca tggtcaatgc aatgggagtg
    13021 aatacaaatc gaatgaagat tattggttta gctctagcaa attccctaac agctttcagt
    13081 ggtagcatcg ttgctcaatt acagcagttt accgatataa acatgggaat tggaattgtg
    13141 attttcggtt taggttcagt tatgattggg gaaacattaa tggatgtttt taaacgtcaa
    13201 ggtattgcct ggcggataat aggagtggtg ataggctgcc tcatttttcg attggttatt
    13261 gcctttactc tagcttctgg agcagacccg aatctactca aacttatcac tgctggtgtt
    13321 gtgctcttat ttgtgagtat tccacggttt aaaggaaaaa cagcattcag atgatcgaaa
    13381 tactcaaact gaataagact tttcacctcg gctccgaacg tcaagtgatt gctgtccaga
    13441 atctggatat ttcatttgaa aatgggtcgt ttaatctttt aataggatca aatggctcag
    13501 gaaaatcaac cctactgaat ctaattgcag gaaccatatt gcctgactcg ggaaccatct
    13561 cgattaacgg aaaaccaata caatcaattc cttcctacaa aaggagcaca tcaatcgctc
    13621 gtatttttca agatccaaca gctggaacgg caccagacct ttccttgctc gacaacttcc
    13681 gtatagcctc actaagaact caaaagaaag ggcttagaat tggcgtggat gcagcatttc
    13741 gtaaagtcgt tgcagcgcat gttagcatcc tagacatggg gctcgaaaat cgactcaatc
    13801 aacccatggg cagtttctca ggcggtcaac ggcaggcgct tacactactc atggccacct
    13861 ttgataaact cgacgtttta ttgttagatg aacctacagc agctttagat cctaagtcgg
    13921 cagtccgtat cttggaacta actcaacgca ttgtagccga aaagaaaata atagccatta
    13981 tggtaacaca tgagcttcgt caatgcattc aggttggaac ccgcattatt caactctccg
    14041 agggccaaat cattcgggat gttgcaggta atactaaaac acaattacga ttagaagaac
    14101 tagctaattg gttcgcataa ccccagactg aggtccgagg ctgccgatct tttatctaat
    14161 attcaactcc ctttccccca gcatccccgg acttcagtcc gggattttca ctccccatca
    14221 caacattttc catcttcccg tgttaagctg aggttaattt ccaccataaa cgctaccttt
    14281 gaaatctatt tccccgtcaa ccaggcctat gaatattatt ccgggagaat tgcttgctca
    14341 gatcgactct cctgccgatc taagacgatt caaagaagac gacctcgtgc aaatctgcaa
    14401 tgagttacgt caatatatca tcgacattgt atctgttaaa ggcgggcact tcggcgcgag
    14461 cttaggaaca gtcgagcttt cagttgcact tcactatgca tttaacactc catatgatca
    14521 acttgtttgg gatgttggtc atcaagctta tgggcataaa atgcttactg ggcgtagaga
    14581 agttttccat acgaatcgag tttatggagg aattagtggc ttccctaaaa gaaaagaaag
    14641 cgaatacgat acatttggtg taggacatag ttctacttct atttctgctg ctcttgggat
    14701 ggctattgcc tctcatttga aggatgaaaa atcacgtcaa catattgcgg ttattggtga
    14761 tggcgctcta actggcggaa tggctttcga agcgctcaat catgcaggtg cttcaaataa
    14821 caacatgtta gttatcctaa acgataactg catgtcgatt gatcctaatg tgggtgctct
    14881 caaagaatac ctcaccgata ttaccacttc acacacatac aaccgtgtga aagatgaggt
    14941 ctggaactta ttagggaaga tttccaaatt cggaccgaat gctcaggcta tcgttcaaaa
    15001 gatagaaaat gcgattaaga gcgctgtttt gcaacaaagc aacttattcg aatcactcaa
    15061 ttttcgctat ttcggtcctg tcgatgggca cgatgtaaat cgtcttgttc aggtaatgaa
    15121 cgacctgaaa gatattcctg gtcccaaatt gctacatgtc cttacggtaa aagggaaagg
    15181 atacaagcta gccgaagaag atcaaacact atggcacgct ccgggacttt tcaataaaga
    15241 cacaggtgaa atcattaaat caccgaaaaa gggaccagaa ccacctaagt atcaggatgt
    15301 atttggtcat actatagttg agattgccaa agaagacaca cgtgtcgttg gaattacacc
    15361 ggcaatgcct tcgggatgtt cgctcaacat tatgatgaaa gagattcccg atcgggcttt
    15421 cgatgtggga atctgtgagc aacatgcggt tactttctct gctggtttag ctactcaagg
    15481 aatgattcct ttttgtaaca tctacagttc gttcatgcaa agagcgtatg atcaggtgat
    15541 acatgatgtg gctatccaaa atttacatgt tgtattttgt cttgatcgtg gcggtgtagc
    15601 aggtgctgat ggcccaactc accatggcgc
//
LOCUS       DAOOQW010000039        15191 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_4779_length_15191_cov_4.970270, whole genome shotgun sequence.
ACCESSION   DAOOQW010000039 DAOOQW010000000
VERSION     DAOOQW010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 15191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15191
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4779_length_15191_cov_4.970270"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..923
                     /gene="trxB"
                     /locus_tag="PK281_04050"
     CDS             <1..923
                     /gene="trxB"
                     /locus_tag="PK281_04050"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007092872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HOY48548.1"
                     /translation="CLIIGSGPAGYTAAIYAARADMKPVMYQGLQPGGQLTITTEVDN
                     FPGYPNGIDGTKMMEDLLAQAQRFGTDVRFGLATKVDFSGPVHKVWVDETKLITADTV
                     IIATGASAKWLGLESENKYNGFGVSACAVCDGFFYRGQEVAIVGGGDTAAEEATYLAK
                     LCKKVTMIIRRDELRASKAMQHRVFNTPNIEVLFNTETLEITGEQSVSGARFKNRVTG
                     EVFTRDITGFFVAIGHEPNSGIFKDYLDMDENGYIKTIPGSSRTNVAGVFACGDVQDH
                     IYRQAVTAAGSGCMAALDAERYLATVGVEA"
     gene            1083..1631
                     /locus_tag="PK281_04055"
     CDS             1083..1631
                     /locus_tag="PK281_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HOY48549.1"
                     /translation="MTTTNSLIHVPDEVIMSKIIVIRDKKVMLDRDIAELYGVSTKRL
                     NEQVKRNPKRFPPDFMFQITQDEKEILIQQFEHLNALKFSSTLPFAFTEHGAVMLSSV
                     LSSEQAIEINIQIVRVYTHIRQYLSDTTAFRLEIEKIKSKLENQSKNIELVFQYLDEL
                     MELQSNEIIRPAIGFQHSLARS"
     gene            complement(1670..3262)
                     /locus_tag="PK281_04060"
     CDS             complement(1670..3262)
                     /locus_tag="PK281_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013070665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HOY48550.1"
                     /translation="MSTITEEIGKRRTFAIISHPDAGKTTLTEKLLLFGGAIQTAGAV
                     KSNKIKNTAASDFMEIEKQRGISVATSVMNFKYKDVLINLLDTPGHKDFAEDTYRTLT
                     AVDSVILVVDCVKGVEEQTEKLMEVCRMRDTPVIVFINKMDREGQNPFDLLDELEAKL
                     NIKVRPLSWPISIGATFKGVYNIFDGSLNLFNANKTKIEEDILTIQSLNDPLLEEIVG
                     KKDADQLRQDIELIEGVYDPFDPIRFREGHIAPVFFGSAINNFGVRELLDTFIRLAPA
                     PRSREADTRIIAPDETKFSGFVFKIHANIDPRHRDRIAFLRVCSGKFERNKFFHHVRL
                     NKDYKFSSPATFMAQEKNIIDEAWPGDVIGLYDTGNFKIGDTLTEGENFQFRGIPSFS
                     PEIFKEVVNADPLKSKQLDKGVQQLTEEGVAQLFVQHNGSRKIIGTVGELQFEVIQYR
                     LKHEYGASCNFQHLNVYKACWVTYDDKEEFEKFARFRGHQLAVDKDGNYVFLAESSWM
                     LNTIIQDFPKLHFHFNSEFKTA"
     gene            complement(3295..5817)
                     /locus_tag="PK281_04065"
     CDS             complement(3295..5817)
                     /locus_tag="PK281_04065"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOY48551.1"
                     /translation="MRIRLSGVLRFFLFVLIAGVSVQLSAQVSNVEFRAKNFPGQEAN
                     FKLADEAYKQGDFYFLRGPIYFPLALKEYLIAQDFNPNNADLNQQIGLCYLSLQIDRL
                     KALPYLERARSLNSNLGNDFLYSLGKAYQYNLDFENAIRVYEGYIAALGAGSDFSKKE
                     KANKSIEECRSGIELVKKPVRVRIDNLGPGVNSAFPDYAPVISEDENKLIFTSRRENT
                     TGGMVDGEDSLYYEDIYITYKIDGDWAPAKNIGEPINKEDHEASINLSADGKKLLIYR
                     TSNGGDIFESKLEGIMWSEPRAMKEINTKGYENHATYSADGKYLFFISVKTDSSGYGG
                     KDIYIADIDENGKAHNIRNAGGLINSAYDEDGVYFHPDGKTLYFSSKGLNSMGGFDVF
                     KATWENEKLSAPINLGYPINSPEDDVFFVLSKDGKRAYFSSYREEGYGDKDIYIMNFL
                     EDAELLSSLQFSIRDSSSDKPMLAHITIKDAATGQIVAERETENGETIANLPAGKTYE
                     ITVSSDKFNSYTEVLEIPFDAGSQVVARKIDLGKSPQTFVSGLLIDKSSRIPVKGEVE
                     FLDAATMEVVKTSFSDKNGEYKIALPPGKEYTIQVKASGYSMSTQNLKLDATSNGNDI
                     VQSFELNRLDRSLMSVPKGRIYDASTGQTLKAEILLNEYGGQPVIMYYKPDSYDCVVF
                     NGATHTMVVNLDGYMTYTAQIHIPETKEKLEVVHDIPLVKAEKGAKIVLNNIFFDFNK
                     STLRPASYKSLNTLLSTLKRYPSMAIEISGHTDNVGTMDYNQKLSDQRAAVVKEYLVR
                     NGIASKRIGSYGRSFRQPIASNDTPEGRQLNRRTEIKILKMK"
     gene            complement(5935..6516)
                     /locus_tag="PK281_04070"
     CDS             complement(5935..6516)
                     /locus_tag="PK281_04070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HOY48552.1"
                     /translation="MNSQILLIDNYDSFTYNLVHLIEMLHGEKPLTLRVDEVNSAHIT
                     NSQAVVISPGPGLPNEMGHLMELVQLAINTKPVLGVCLGHQAIAEAYGGKLNQLAQVY
                     HGIAREAQVEISIPMYRDCPKTFEAGSYHSWTASDNDFPKELIVTARDNQREILSFIH
                     ESKPIWGVQYHPESILTPQGPTIVRNWLDFALK"
     gene            6571..7914
                     /locus_tag="PK281_04075"
     CDS             6571..7914
                     /locus_tag="PK281_04075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48553.1"
                     /translation="MSTINLGTFLRCIVLIGSIQTIQAQTGLNKPFRSDISFDYGFIR
                     FKGDLATSPYGIGNYSIGGSFHQSYKAWKADFQVAQSLITWNEQASLNRNNFQAKSMR
                     YGLLLGRYLFNPNTENFCNPYLLLGISSIQFNSYTDLKDENGLDYFYWKDGTIRDLPE
                     ASFNQTTSKILRRDYQYESPLAINQQSICFPLELGLRANVSPRMAVQFSWQSVFIQSD
                     NIDRNTDNPGWDNIHQITASVCVSLNTVRKVEPKKSNHPVIDYQDVDYQALLSTDEDA
                     DGVLDVNDRCYGTPKGAPVDEFGCMQDEDQDGIPDYKDLEKTTASGAWTDTLGRTYSD
                     AWLQEHYSDSTSYFVQVLRKINRNSRPYPVRKYIPESNIQRWNQLLEEHPEWRTVNYG
                     KSSEMPEEIRVVDANHDQFISLKELEKAANDLFDGKKGITREILEKAMIYAFGDQ"
     gene            7895..8326
                     /locus_tag="PK281_04080"
     CDS             7895..8326
                     /locus_tag="PK281_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48554.1"
                     /translation="MRLVISSISFILAALSSWAQPTISLNGPKQARFGLPTVIKTEIV
                     RNSTTGPVRLVTELPQGWILRSSDSNLGLINQEGNLVKTVWLNLPLQDTIRHSLLVII
                     PENHSGDAVIKAHLEYYSGDNIHHIACSPFAIEVRKYYSRY"
     gene            complement(8323..10413)
                     /locus_tag="PK281_04085"
     CDS             complement(8323..10413)
                     /locus_tag="PK281_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48555.1"
                     /translation="MSLEFVFQYPAWFLVFCLILGFLYSWLLYRKGKELKEAKPWLKK
                     LVFGLRFLAVSIIAFLLLSPLIKLINRESERPVIVLLQDNTGSIRQVKDSAFYKKEYP
                     GLIEDLREKLEETYDFRSYSFDNTLNDSFRLDFQGKETNLSEAMQEINTLYLNRNVGA
                     LIVASDGIYNQGSNPLYIPNEIKAPIYTIAMGDTTLQRDLMIARINHNQLAYLDNTFP
                     VEVVVNANKLPGKNSVLKIERDGVVLATQPISISNQAFIQTYPFQLKADKSGVLRYKV
                     SLSTVDGEFTLANNTREFFIEVIDSRQKILILADAPHPDVAAIRQSLQSNGNYEVEVK
                     LASEFTTSVKPYSLVILHQLPSKTNMIQRVVQELDNSNVPAWFIAGNSSLPGALNTVQ
                     PLVQFLSGKGSMNDASPRLNKDFALFTLSDDLRKAFNTFEPLQVPYANYRVQPSAAVM
                     LRQKIGSVETDYPLLAFSSDATRKTGILLGEGIWRWRLQDYLEHDDQNLFNEFISKIV
                     QYLSVRNERKNFRIVSKNSFNENEQVTFEAEVYNASYELVNTPDVLVNVFDSENRKYA
                     YSMSKTTTAYSLNAGIMRPGDYRYEASVVLAGKSYTAAGRFVVKPVLAELSSITADHT
                     LLRTLAIRNSGELFLPRDLVKIAERIQAREDVKPISRSQVSLKELIQLKWIFFLILIL
                     LGTEWFLRRRNGSY"
     gene            complement(10451..11197)
                     /gene="fabG"
                     /locus_tag="PK281_04090"
     CDS             complement(10451..11197)
                     /gene="fabG"
                     /locus_tag="PK281_04090"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015782609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HOY48556.1"
                     /translation="MKLLQDKVAIITGATRGIGRGIALKFAEQGANVAFTYMSSAQKA
                     LELEEELKALGVKAKGYHSDAAEYAAAEALVNDVVAEFGTLHIVVNNAGITKDGLLMR
                     MSEEQWDAVIHTNLKSVFNLTKAAIKPMLKNRYGAFVNMSSVVGGSGNAGQANYAASK
                     AGIEGFTKSVAQELGSRNIRSNAIAPGFIETEMTAELSDDVKGKWIEGIPMKRGGTPE
                     DVANATVFLASDMAAYVNGQVLNVCGGMRT"
     gene            complement(11439..12245)
                     /locus_tag="PK281_04095"
     CDS             complement(11439..12245)
                     /locus_tag="PK281_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48557.1"
                     /translation="MKSNKLLIDVPLPCSETWDEMQAVDAGKYCKSCAKHVTDFAGFT
                     DQELIRYFSTISGETCGRFRVEQLNRKIVATHVNRNSIQPRGLWLAGLMGLSGLTIHA
                     QKQEPNQKKEACVQVKPIQVKEGMGVYSFKDDHFHGQAIGNNQQVLPGAVISYVEGRI
                     AKGLIADNLGRFTIQRGDFRDSVITLTVMANEEGLKHISMVEEMKTIEIIVDKSRWDD
                     GDYVVGKYPQGRDPIYLQIEKRNRTQLFFYKLFHYRKWLKWKRLQKAASQ"
     gene            complement(12232..12996)
                     /locus_tag="PK281_04100"
     CDS             complement(12232..12996)
                     /locus_tag="PK281_04100"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOY48558.1"
                     /translation="MKTNLPSGAQIKIPNPCREDWTGMELTESGRFCNQCEKSVMDFT
                     HYTDQQLSEYLDRNRHTPICGRIPISKLNKPLEVPPVKYHYKGWWIWAALAGSSLTLQ
                     AQMESEMVQVADHNKDIELSIHVEADSGFIHVKGFLEDRGTSERLPFAFIEFKQKSVK
                     CVTDVNGEFVLQIPANLKPDEKIELTISYVGYKTKRIDFVYSQLVALQGSALQLQLDL
                     AENTIIGEIIYIRPPWHKRVWYKVKRIFKHKPREIE"
     gene            complement(13147..>15191)
                     /locus_tag="PK281_04105"
     CDS             complement(13147..>15191)
                     /locus_tag="PK281_04105"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013304653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HOY48559.1"
                     /translation="SKALNVSFTGGAVMGLGVSGLAVLGLGGLYIVLKQYFAPGATVD
                     SEEMIRTIEVLTGFSLGAESIALFARVGGGIYTKAADVGADLVGKVEAGIPEDDVRNP
                     ATIADNVGDNVGDVAGMGADLFGSYVATILATMVLGQEIDASTDNFGGMSPILLPMLI
                     CGLGIIFSIIGTWFVTIKDEKSNVQNALNLGNWSSMLLTVVASYFIVMWMLPETLDLR
                     GYKFSNINVFFAIVVGTVVGAVMSIVTEYYTAMGKAPVNSIIQQSSTGHATNIIGGLS
                     VGMKSTVIPIITLAAGIMASYHFAGLYGVAIAAAGMMATTAMQLAIDAFGPIADNAGG
                     IAEMSQLPPEVRERTDNLDAVGNTTAATGKGFAIASAALTSLALFAAFVGIAGISAID
                     IYKAPVLAGLFIGGMIPFIFSALCIQAVGKAAMDMVQEVRRQFREIPGIMEYKAKPEY
                     EKCVAISTKASIREMMLPGAIAIVTPVVVGFIFGPEVLGGLLAGVTVSGVLMGIFQSN
                     AGGAWDNAKKSFEKGVMINGEMFYKKSEPHKASVTGDTVGDPFKDTSGPSMNILIKLM
                     SIVSLVIAPYIAVVATTNTDEACMPGTCNHEMMEAHCKQSGVCAATGKACTWNDSTNT
                     CAHNDGTVCNQMNDASAKACCSPEGEMKAECKTGDKKDCCKPGAEAATAKKDCCKK"
BASE COUNT         4599 a         3287 c         2738 g         4567 t
ORIGIN      
        1 aatgtctgat cataggatca ggacccgctg gatatacagc agcaatttat gccgcacgtg
       61 ccgatatgaa accggttatg tatcaaggtt tacaacctgg cggacaatta acaattacca
      121 ccgaggtgga caatttccct ggttatccaa atggaattga tggtactaaa atgatggagg
      181 atttgttagc tcaagcacaa cgttttggaa cagacgttcg ctttggatta gcaacaaaag
      241 ttgatttctc tggtccggtt cataaagttt gggtcgatga aaccaaatta atcacggctg
      301 atactgtgat tattgcgaca ggtgcatcag ctaaatggtt gggtttagaa tcagaaaata
      361 aatacaatgg ttttggtgtt tctgcttgtg cagtatgtga tggattcttc tatcgcggac
      421 aagaagttgc aatcgttgga ggtggcgata ctgctgcaga agaagctacc tatctagcta
      481 aattgtgtaa aaaagtaacg atgataattc gtcgggatga attacgtgct tcaaaagcca
      541 tgcaacatcg cgtattcaat actccaaaca ttgaagtact attcaatacc gaaacacttg
      601 aaattactgg tgagcaatcg gtaagtggag cgcgtttcaa gaatcgtgta acaggtgaag
      661 tatttacacg tgatataaca ggtttctttg ttgcaattgg acacgaacca aactctggaa
      721 tttttaaaga ttatttagat atggatgaaa atggctatat caaaacgatt ccgggaagtt
      781 ctcgtacaaa tgtagctggt gtttttgcat gtggtgatgt gcaagatcat atctatcgtc
      841 aagctgtaac tgctgctggt agtggttgta tggctgcttt agatgctgaa cgctatttag
      901 ccacagttgg tgtagaagcc tgatattaac atacttgttc ataaaaagcc atgtagaatc
      961 tgcatggctt tttttatgtt tacaattctg aagctgccta taagctatcg gaagattatt
     1021 agaaaccact tattcattta tacataggcg gtcgcatatt gcgaccacct tcaattttaa
     1081 atatgactac aaccaatagc ttaattcatg tgcccgatga ggttatcatg agcaaaatca
     1141 tcgttattag agataaaaag gtaatgcttg atcgagatat tgcagaactt tatggagtaa
     1201 gcaccaaacg attaaacgaa caagtaaaac gaaatcctaa aagatttccg cctgatttca
     1261 tgtttcaaat cacccaagat gaaaaagaaa tcttgattca acaatttgaa catttaaatg
     1321 cgctcaaatt ctcctctact cttccttttg cctttaccga acatggtgct gttatgcttt
     1381 ccagtgtact ttcaagtgaa caggcaattg agataaatat tcaaattgtt cgggtataca
     1441 cacacatccg tcagtattta agtgatacca cagcttttcg tttggaaatt gaaaaaatca
     1501 aaagcaaatt ggaaaatcaa tctaaaaaca tcgaattagt tttccaatat ctagatgaat
     1561 taatggagct acaatccaat gaaattattc gtccagctat aggtttccaa catagtttag
     1621 cacgatcata atcacgaata ttagtcgtga cagaaatatt ccagcctaat tatgcagttt
     1681 taaattccga attaaagtgg aaatgtagct taggaaaatc ctgaatgatg gtgtttaaca
     1741 tccaagaaga ttctgctaag aacacgtaat tcccatcttt atctacagct aattgatgac
     1801 cacgaaaacg agcgaatttt tcaaattcct ctttatcatc ataagttacc caacaagctt
     1861 tgtacacatt caaatgctgg aaattacatg aagctccgta ttcatgtttg aggcgatact
     1921 gaattacttc aaactgaagt tcaccaacag ttccgattat tttccggctt ccattatgtt
     1981 gaacaaaaag ctgcgctact ccttcttcgg ttaactgctg aacaccttta tctaactgct
     2041 tcgatttaag cggatcggca ttgacaactt ctttgaaaat ctccggtgag aaacttggaa
     2101 ttcctctgaa ttggaaattt tcaccttcag taagtgtatc accaattttg aagtttccag
     2161 tatcgtataa tccaatcaca tcgcccggcc aagcttcatc aataatgttt ttctcctgag
     2221 ccatgaatgt tgcgggagaa ctgaatttat agtctttatt aagtcgaacg tgatgaaaga
     2281 atttattccg ttcgaacttc cctgaacaaa cacgtaaaaa ggcaattcgg tcgcggtgac
     2341 gaggatcaat attcgcgtga atcttaaata caaatccact aaatttagtt tcgtctggtg
     2401 ctataattcg agtatctgct tcgcgacttc tgggtgctgg tgctaaacga ataaacgtat
     2461 cgagtaattc acgtacacca aaattattga ttgccgaccc aaagaaaacg ggagcaatat
     2521 gtccttcacg aaagcgaatt ggatcaaatg gatcgtatac accttcaatc aattcaatat
     2581 cctgacgaag ttgatctgca tccttcttcc ctactatttc ttctagtaat ggatcattta
     2641 aactttgaat agtgagaata tcttcttcaa tcttggtttt attcgcgttg aagagattta
     2701 aactaccatc gaaaatattg taaacacctt taaatgttgc acctatactg attggccaac
     2761 ttaaaggacg aactttaatg ttcaattttg cttcaagctc atccaaaaga tcaaaaggat
     2821 tttgtccttc gcgatccatt ttattgataa acacaatcac gggagtatcg cgcatacggc
     2881 acacttccat gagtttttcg gtttgttcct ctaccccttt cacacagtca acaaccaaaa
     2941 taacgctatc tacagcagtt aaagttcggt aggtatcttc tgcaaaatcc ttgtggccgg
     3001 gagtatccaa caagttgatg agtacatctt tgtacttaaa attcatcacc gaagtagcca
     3061 ccgaaatacc acgttgcttc tcgatttcca tgaagtcgga tgctgcagta ttcttaatct
     3121 tattcgattt aaccgcacca gccgtttgaa tagcaccacc gaataacagt aatttttcgg
     3181 ttaatgtagt cttcccggca tcggggtgag agatgatagc aaatgtgcgg cgcttgccga
     3241 tttcttcggt aattgtactc atacaggcgg caaaaatagc aaatcctagc gcccttattt
     3301 catctttaat attttgattt cagtacgacg gttcaattgg cgtccttcgg gtgtatcatt
     3361 actagcaatt ggctgacgga atgaacgtcc ataagaacct atgcgtttag atgcaattcc
     3421 atttctaact aagtattctt taactactgc agcacgttga tcgctcagtt tttggttata
     3481 atccattgtt ccaacattat cggtatgtcc cgagatttcg atagccatgg atggataacg
     3541 cttaagtgtt gacagcaatg tattcaatga tttataagaa gccggacgaa gcgttgattt
     3601 gttgaaatcg aagaaaatat tgttcaacac aatctttgcc cctttctcgg ctttcaccaa
     3661 aggaatatca tgaacaactt ccagtttttc ctttgtttca ggaatatgaa tttgtgcggt
     3721 ataagtcata tacccatcta gattcaccac cattgtatga gttgcaccat tgaataccac
     3781 acaatcgtat gaatcgggct tgtaatacat gattacaggt tgaccgccat attcatttaa
     3841 tagaatttca gctttaagtg tttgaccagt tgatgcatca taaattcgcc ctttcggcac
     3901 cgacatcagt gaacgatcaa gacgatttag ctcgaatgat tgaacgatat cgttaccatt
     3961 agaagttgca tcgagcttaa gattttgagt cgacatggaa taaccactag ccttaacttg
     4021 aatggtatat tctttacctg gaggaagtgc tattttatat tctccatttt tatcgctgaa
     4081 agatgtttta acaacttcca tagtagctgc atcgagaaac tccacttctc cctttacagg
     4141 aattcgggaa gatttatcta ttagtaatcc tgacacaaac gtttgtggcg atttacctaa
     4201 atctattttt cgagcaacaa cctgagaacc tgcatcaaac ggaatttcaa gcacttcggt
     4261 gtaactgttg aatttgtcgg atgaaacagt tatttcatac gttttacctg ctggaagatt
     4321 tgcaatcgtt tccccatttt cggtttcacg ttcggctaca atttgtccgg tagcagcatc
     4381 tttaatcgtg atatgtgcca acatgggctt atcactactt gaatcgcgaa tgctaaattg
     4441 aagtgagctc aataactcag catcttccaa gaaattcatg atgtaaatat ccttgtcgcc
     4501 atatccttct tcccgataag aagaaaaata agcacgtttc ccatccttcg acaaaacaaa
     4561 aaacacatca tcttctggtg agttaattgg atagcctaaa ttgataggag cacttaattt
     4621 ttcgttttcc caagtagctt taaatacatc gaatccaccc attgaattca atcctttaga
     4681 agagaaatac aaggtttttc catcgggatg aaaatacact ccatcttcat cgtaagccga
     4741 attaatcaat ccacctgcat tacgaatgtt atgcgcttta ccattttcgt caatatcagc
     4801 aatgtaaata tccttgcctc catatccact tgaatcggtt ttcacggaaa tgaaaaacag
     4861 gtacttacca tcagctgaat aggttgcgtg gttttcgtat cctttcgtat tgatttcttt
     4921 cattgcacgt ggctccgacc acataatacc ttccaatttc gattcgaaaa tatctcctcc
     4981 attgctggtc cggtaaatca aaagcttttt cccatcagcc gaaagattga tactcgcctc
     5041 atgatcttct ttattaattg gctcaccaat gttttttgct ggtgcccagt ctccatctat
     5101 tttgtatgtg atatagatat cctcgtagta aagtgagtct tctccgtcca ccattcctcc
     5161 tgtcgtattc tcacggcgag acgtaaaaat taatttgttt tcatcttccg aaataactgg
     5221 agcataatca ggaaatgcag agttcacacc cggtccaaga ttatcaatac gaacacgaac
     5281 aggcttttta accaattcaa ttccagaacg acattcttca atagatttat tagctttctc
     5341 ttttttagaa aaatcagagc cagcacctaa agccgctata taaccttcat acactcgaat
     5401 tgcattctca aaatcgagat tgtattggta ggcttttccc aaagaataaa gaaaatcatt
     5461 ccccaaatta ctattcaacg aacgcgcacg ttctaaataa ggcaatgcct tcaaacgatc
     5521 aatttgtaaa ctcaaatagc ataacccaat ttgttgattt aagtcggcat tattgggatt
     5581 aaaatcctga gcaattaagt attcctttaa tgctagagga aaataaatgg ggccacgtag
     5641 aaagtagaaa tcaccttgct tgtaggcctc atctgctagt ttgaaattag cttcttgacc
     5701 gggaaaattt tttgcccgaa actcaacatt gctgacctga gccgacaatt gaacggaaac
     5761 acctgcgatt agcacaaata agaaaaatcg caatactccg cttaaacgaa tgcgcatggg
     5821 tcgattttat agggtttatt agacgaaaat aatgtgttaa aatcaactta acatgttttt
     5881 tacgactaaa atcgacttga ttaccaacat gcaaatgttg atgagtaaca ctccctattt
     5941 tagcgcaaag tcaagccagt tgcgaacgat agtaggtcct tgaggtgtta atattgattc
     6001 gggatgatat tgaactcccc aaataggttt actttcatga ataaatgaaa gtatttctct
     6061 ctgattatcg cgagcagtta cgatcaactc tttaggaaaa tcattatcac ttgctgtcca
     6121 ggaatgataa ctacccgctt caaatgtttt tggacaatct cgatacattg gaatggatat
     6181 ttcaacctga gcttcacgag cgataccatg atacacctga gctaattgat tgagttttcc
     6241 accgtatgct tcagctattg cctgatgacc aagacaaacg cctaaaacag gttttgtatt
     6301 aattgcaagc tgaaccagtt ccatcaaatg tcccatttcg tttggcaaac ctggtccggg
     6361 agaaataaca acagcttgag aattggtaat atgtgcagaa ttcacctcat caacacgaag
     6421 cgttaaaggc ttctccccat gaagcatctc aattagatgt accaaattat aggtaaatga
     6481 atcgtaattg tcgatcaaaa gtatctggct attcatggga aatttttact cgcttccgga
     6541 aagatatttt tctgcaattt tacgccattg atgagcacaa ttaacctggg gactttttta
     6601 cgttgtattg tacttattgg aagtattcaa actatccaag cacagactgg tttaaataaa
     6661 ccatttcgtt cggatatttc attcgactat gggtttattc ggtttaaagg tgatcttgct
     6721 acgagtcctt atggaattgg aaattacagt attggcggtt cattccatca atcctataaa
     6781 gcttggaaag ctgattttca agtagctcaa tctttaatta cttggaacga acaagctagc
     6841 ctgaatagaa ataattttca agctaaatcg atgcgttacg ggttattgct tggtcgatat
     6901 ttgtttaatc ctaacacgga gaatttctgc aacccatact tacttctcgg tatttcgtcc
     6961 attcagttta attcgtacac cgatttaaag gatgaaaacg gacttgatta tttttattgg
     7021 aaagatggaa caatccgaga tttaccggag gcatcattca atcaaacaac ttccaaaatc
     7081 cttagacgtg attatcaata tgaatcacct ttagccatca accaacaaag tatttgtttt
     7141 cctttagaat tgggattaag agccaatgtt tcaccacgaa tggctgttca atttagctgg
     7201 caaagtgttt tcatacaatc cgataatatc gatcgcaata ccgataatcc aggttgggat
     7261 aatattcacc agattacagc tagtgtctgc gtttcgctta atacagttcg taaggttgaa
     7321 ccaaagaagt caaatcatcc tgttattgat tatcaagatg tggattatca ggctttactt
     7381 tcgacggatg aagatgcaga tggagtttta gatgtaaatg atcgttgcta tggtactccc
     7441 aaaggtgcgc ctgttgatga atttggctgc atgcaagatg aagatcagga tgggattcca
     7501 gattataagg atttagagaa aacaacagct tcgggggctt ggacagatac tttaggtcga
     7561 acctattcgg atgcatggtt acaagaacat tattccgatt ctaccagtta ttttgttcaa
     7621 gtacttcgaa aaataaaccg aaattctcgc ccatatccag ttcggaaata cattcctgaa
     7681 tccaacattc agcgctggaa tcaactgtta gaagaacatc ccgaatggcg tactgttaac
     7741 tatggaaaat caagcgaaat gcctgaagag attcgtgttg tagacgctaa tcacgatcag
     7801 tttatcagcc tgaaagaatt agaaaaagcc gccaacgatt tattcgatgg taaaaaaggt
     7861 atcacccgag aaattctcga aaaagctatg atttatgcgt ttggtgatca gtagtatcag
     7921 tttcatatta gctgctctgt catcttgggc acagcctacg atttcgctaa atgggcctaa
     7981 gcaagcaagg tttggactac cgactgtaat taaaactgaa atcgttagaa attcaacaac
     8041 tggtcctgtt cgattagtaa ctgaattgcc tcagggctgg attttacgaa gttcagacag
     8101 caatctaggg ttaataaatc aagaaggaaa tctggttaaa acagtatggt tgaatttgcc
     8161 cttacaggat accattcgtc attccttatt agttattatt cccgaaaatc attccggaga
     8221 cgcagttatt aaggctcatt tggaatatta ttcaggtgat aatattcatc acatagcatg
     8281 tagtccgttt gctatcgaag taagaaaata ctattctcga tattaatagg atccgttcct
     8341 tctccttaga aaccattccg ttcctaataa aatcaaaatc aggaagaaaa tccacttgag
     8401 ttgaatcaat tctttcaagc taacctgact tctagaaatc ggctttacat cttctctagc
     8461 ctgaattcgc tcagcaatct ttaccagatc acgcggcaaa aacaactcgc ccgaattgcg
     8521 aattgctaat gttcttagta aggtatgatc ggctgtaata gaacttaact cagctaaaac
     8581 aggctttacg acaaatcgcc cagctgctgt ataactctta cccgctaaaa ccaccgaagc
     8641 ttcataccgg taatcacccg gacgcataat accagcattc aacgaatagg ctgtcgttgt
     8701 tttactcatg ctgtaagcgt atttgcgatt ctccgaatca aacacattca ccaatacatc
     8761 gggcgtattc accagctcat aagaagcatt atacacttca gcttcgaatg taacttgctc
     8821 attttcgtta aaactgtttt tactaacgat tcggaagttt ttacgttcgt ttcgtacact
     8881 cagatattga acgattttag aaataaattc attaaataag ttctgatcat catgctccaa
     8941 ataatcctgc aatctccagc gccaaatacc ttctcccaat aaaattcctg ttttccgagt
     9001 tgcatccgaa gaaaaagcca ataaaggata atccgtttct actgagccaa ttttttgacg
     9061 caacataaca gctgccgatg gttgaacacg atagttcgca tacggaacct gcaaaggctc
     9121 aaacgtgtta aatgcttttc ttaaatcatc gctaagcgta aataatgcaa agtccttatt
     9181 taaacgtggc gatgcatcat tcattgaacc tttgccactc agaaattgaa cgagcggttg
     9241 aacggtattt aatgcaccag gcaaggaaga attcccagca ataaaccaag ctggaacatt
     9301 cgaattatct aactcctgaa ccacacgttg aatcatgttg gtcttgctcg gaagttgatg
     9361 caaaataacc aaactgtatg gtttcacact ggtggtaaac tccgaagcta acttcacctc
     9421 tacttcataa ttcccgttcg attgcaaact ttgacgaatc gctgcaacat ctggatgtgg
     9481 tgcatcagca agaattaaga tcttttgccg actgtcgatt acttcaataa aaaactcgcg
     9541 agtattattg gctaaagtaa attcaccatc aacagtactc aaactcactt tatacctcaa
     9601 aacacccgat ttatcagcct taagctgaaa tggataggtt tgaataaatg cttgattgct
     9661 aattgaaata ggttgagttg ccagcactac tccatctcgc tcaattttca ataccgaatt
     9721 ttttccgggt aacttattgg cattcaccac tacttcaacc ggaaacgtat tatcgagata
     9781 agctaattga ttgtgattaa ttcgggcaat cattaaatcg cgctgcaggg ttgtgtcacc
     9841 catggcaatg gtgtaaatcg gcgctttaat ttcgttagga atgtatagcg gatttgaacc
     9901 ttgattgtaa ataccatcgc tggcaacaat caaagccccc acattgcgat tcaaatacag
     9961 ggtattgatt tcctgcatgg cttccgacag gttcgtttcc ttgccctgaa aatccaaacg
    10021 gaacgaatcg ttgagcgtat tatcgaaact gtatgaccta aaatcgtagg tctcttctag
    10081 cttttcacgt aaatcctcaa ttaatccagg atattctttt ttgtagaaag ccgaatcttt
    10141 cacctgacga atcgaaccgg tattatcttg caacagcaca attaccggac gttcactttc
    10201 ccgattaatc aatttaatca gaggagagag caacaaaaaa gcaataatcg aaacggccaa
    10261 gaaacgtagt ccaaaaacca atttcttcag ccaaggcttg gcttctttga gttctttgcc
    10321 cttgcggtat aaaagccagg aatacagaaa tcccagtatc aggcaaaaaa ccagaaacca
    10381 ggcaggatac tgaaaaacaa attcgaggct caaaagaaaa tccggttaat tcaactaaaa
    10441 ctaattacgc ttaggtgcgc attcctccac aaacgttcaa aacttgtcca ttgacatacg
    10501 ccgccatatc cgaagctaaa aaaactgttg cgttcgccac atcttctggt gtaccgccac
    10561 gtttcatcgg aatcccttct atccatttac ctttcacatc atcgcttaat tcggcagtca
    10621 tttcggtttc aataaatccg ggagcaatgg cgttgctacg aatatttctt gagcctaatt
    10681 cttgagctac cgatttggta aatccttcaa ttccagcttt cgaagcagcg tagttggctt
    10741 gtccagcatt tccacttcct cctacaaccg aactcatatt gacaaacgcc ccatagcgat
    10801 tcttaagcat gggtttgata gctgctttgg ttaggttaaa aaccgacttc aaattcgtat
    10861 gaatcacggc atcccattgt tcttcactca tacgcatgag caaaccatcc ttggtaattc
    10921 cggcattatt taccacaatg tgaagcgtgc caaattcagc cactacatca ttcaccaaag
    10981 cttctgcagc agcatattcg gccgcatccg aatgatagcc ttttgctttt acgccaagag
    11041 ctttcaattc ttcttcgagt tctaaagctt tttgagcact cgacatgtat gtaaatgcta
    11101 cattggcgcc ttgttcagca aattttaaag cgataccgcg accaattccg cgggtggcac
    11161 cagttataat agccactttg tcttgaagta atttcatgcg tatacgttct gagccccaaa
    11221 cctacatgaa ttcgggcgca tagccaagtt ccgactttcc aatttttcag taaaaaacaa
    11281 acaatgtttc gtgattaaaa tttgggcggt cgagccggct atccgctact agtcctcgct
    11341 gtaagccttc gcataagctt cggcttaggc tccgggctat ccgcttctat ccgtaccgct
    11401 ctgtgctcaa ctaaatctaa tccgataaac ctgaataatt actgcgaagc agccttttgc
    11461 aaacgcttcc acttcagcca cttacgataa tgaaacaact tataaaaaaa cagttgtgtt
    11521 cggtttcgtt tttcaatctg aagataaatg gggtctctac cttgtggata tttccctaca
    11581 acataatctc catcatccca acggctttta tctacaataa tttctatcgt tttcatctcc
    11641 tccaccatgc taatgtgctt caaaccttct tcgtttgcca tcactgttag cgtaataacg
    11701 gaatctctaa aatctcctcg ttgaatggta aatcgaccca aattatccgc aattaaaccc
    11761 ttagcaattc gtccttccac ataagaaatc acagctcccg gcagcacctg ttgattgttt
    11821 ccaattgctt gtccgtgaaa atggtcatct ttaaacgaat acactcccat tccttctttc
    11881 acctgaatgg gtttcacttg cacgcaggct tcctttttct gattcggctc ttgcttttga
    11941 gcgtgtatcg ttaatccact taagcccatc aaaccggcaa gccatagtcc tcgaggttga
    12001 atactattcc gatttacatg agtagctaca atcttacggt tcaactgttc tactcgaaat
    12061 cgtccacagg tttcacctga aatagtgctg aaatagcgaa tcaactcctg atcggtaaag
    12121 ccagcaaaat cggttacatg ttttgcgcag ctcttgcaat acttaccggc atcaacagct
    12181 tgcatttcgt cccaagtttc cgaacaggga agcggtacat ctataagtag cttattcgat
    12241 ttcacgaggc ttgtgtttga aaatccgttt caccttatac caaacgcgtt tatgccaagg
    12301 tggtcttata taaatgattt caccaatgat ggtattttct gctaaatcca attggagttg
    12361 caatgcagat ccttgtaatg ccactaattg tgaataaacg aaatcgattc gtttcgtttt
    12421 ataacccaca tatgaaatag tcagttcaat tttttcatcc ggtttcagat tagctggaat
    12481 ctgcaaaaca aattccccat tcacatccgt aacacatttg acacttttct gtttgaattc
    12541 tataaaagca aatggcaatc gctcactggt tccccgatct tctagaaatc ctttcacgtg
    12601 tataaaaccc gagtcggctt caacatgaat agataattca atgtccttgt tatgatctgc
    12661 cacttgaacc atttctgatt ccatttgagc ttgaagagtc aaactactac cagcgagtgc
    12721 tgcccaaatc caccagcctt tatagtgata ctttactgga ggaacttcca aaggtttatt
    12781 cagcttcgaa atgggaattc gtccacatat aggtgtatgc cgattccgat ctaaatattc
    12841 gctcagttgc tgatccgtat aatgggtaaa atccatcacc gatttttcgc attgattaca
    12901 aaacctaccc gattctgtta attccatccc cgtccaatct tcccgacatg gatttggtat
    12961 cttaatttga gcacctgatg gaaggttcgt tttcataaat cgtaaacgaa actagctgaa
    13021 gtaaatcgct atcccaaaaa caaaaaaggc cctccatcgc tggaaagcct ttttaagtat
    13081 tatgaattaa gtattatgta ttaagacaag ttcgtatact ttgtactttc tactttgtac
    13141 ttaatactat tttttgcagc aatctttctt agcagtagct gcttcagctc caggtttgca
    13201 gcaatctttt ttatctcctg ttttgcattc tgctttcatt tcaccttctg gtgaacagca
    13261 agctttagct gatgcatcgt tcatttggtt gcaaacagtt ccgtcgttgt gagcacatgt
    13321 gtttgttgaa tcgttccatg tacatgcttt tccagttgca gcacaaacac ctgattgctt
    13381 gcaatgagct tccatcatct cgtgattaca agtacctggc atacaagctt catcagtatt
    13441 cgttgtagca actacagcga tgtaaggagc aataaccaaa gaaacgatac tcatcaattt
    13501 aatcaagata ttcatcgatg gaccagaagt atctttgaat ggatctccaa cagtatcacc
    13561 tgttacagat gctttatgtg gttctgattt cttgtagaac atttcgccat taatcatcac
    13621 acctttttcg aacgattttt tagcgttatc ccaagcacca cctgcgtttg attggaaaat
    13681 ccccatcaat acacctgata ctgttacacc tgctaataaa cctcctaata cttcaggtcc
    13741 gaaaataaat cctactacaa ctggagttac gatagcaatt gcaccaggca acatcatttc
    13801 gcgaatagaa gctttagtcg aaatagccac acatttctca tactcaggtt ttgctttgta
    13861 ttccatgata cctggaattt cgcggaactg gcgacgaact tcctgaacca tgtccatagc
    13921 agctttacca accgcttgaa tacacaatgc cgagaaaatg aaaggaatca taccgccgat
    13981 aaacaaacca gctaatacag gtgctttgta aatatcgata gccgaaatac ctgcaatacc
    14041 tacgaatgca gcgaaaagtg ctaatgaagt taaagcagct gaagcaatcg cgaaaccttt
    14101 accagttgca gcagttgtgt taccaacagc atctaaatta tcggtacgct cacgaacttc
    14161 tggaggaagt tggctcatct cagcaatacc gcctgcatta tccgcaatcg gaccgaatgc
    14221 atcaattgcc aattgcatag cagtagttgc catcatacca gcagcagcga tagccacacc
    14281 gtataatcca gcaaaatggt aagaagccat gataccagca gctaaagtaa taattggaat
    14341 taccgttgat ttcatcccta cagataaacc accaataatg ttcgttgcgt gacctgtaga
    14401 agattgttga atgatggaat taactggagc tttacccatt gcagtgtaat actcagtaac
    14461 aatactcatg acagctccta caacagttcc aacaacgatt gcaaaaaata cgtttatgtt
    14521 agagaattta tatccacgta aatcaagcgt ttctggtaac atccacatta caataaagta
    14581 agaagctact acagttaata acatcgaaga ccagtttcct aggttcaatg cattttgcac
    14641 gtttgatttc tcatccttaa tggtaacaaa ccaagtcccg ataatagaga agataattcc
    14701 taaaccgcaa atcaacatcg gtaataggat tggtgacatt ccaccaaaat tatcagtgct
    14761 agcgtcaatt tcttgaccta ataccatggt agccaaaatg gttgctacat aagaaccgaa
    14821 taaatcggca cccataccag ctacgtcacc cacgttatca cccacgttat ccgcaatagt
    14881 tgccggatta cgaacatcat cttccggaat accagcttcc actttaccta ccaggtcagc
    14941 acctacgtca gctgccttgg tatagatacc gccacccaca cgggcgaaaa gggcaatcga
    15001 ctcagcaccg agggagaaac cggtcagtac ctcaatcgta cggatcattt cttctgaatc
    15061 cacggtagca ccgggcgcga aatactgttt taatacgata tataaaccgc caaggccgag
    15121 aacagccagg ccggatacac ccagacccat aacggcaccg ccggtaaagg agacattgag
    15181 tgctttactt a
//
LOCUS       DAOOQW010000040        15164 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_4795_length_15164_cov_5.702363, whole genome shotgun sequence.
ACCESSION   DAOOQW010000040 DAOOQW010000000
VERSION     DAOOQW010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 15164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15164
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4795_length_15164_cov_5.702363"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..814)
                     /locus_tag="PK281_04110"
     CDS             complement(<1..814)
                     /locus_tag="PK281_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48560.1"
                     /translation="MSPLQILLPAILCVLTVSLHATSYVWTGSVDTQWGEPNNWSPAG
                     IPDIGDDVTITNQTNDPVFEEISGLNNFTMTSGSLDLSAFTLVIYGTASFDGGSITNG
                     NIAFVSGSVCTFAGTTLSCGISGTMLNVYFNGGTFNNPVSITRTSATDNTSNGGNTYN
                     STFSLTNTGTGNVILSNVSADTYKSDATFSASNTGLIQVSYTASGNSFQGGLLFNSTG
                     TSLGIRLGQNGGTSTLSSGNVLGIGGSGFSVGDLRIKGLTQSGTTAQSLTLTG"
     gene            complement(1114..3057)
                     /gene="ispG"
                     /locus_tag="PK281_04115"
     CDS             complement(1114..3057)
                     /gene="ispG"
                     /locus_tag="PK281_04115"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate
                     synthase"
                     /protein_id="HOY48561.1"
                     /translation="MITLPQIPIYCNSLTSYERFRTREVMIGDLALGGDNPIRLQSMT
                     TTDTLDTAATVAQSIRMIEAGCELVRITAPSIKEAQNLENIRRELRAKGYNTPLVADI
                     HFTPNAAELAARIVEKVRVNPGNYADKKKFEHIEYTDSTYEAELDRIRERFTPLVKIC
                     KEYGTAMRIGTNHGSLSDRIMSRYGDSPLGMVESAMEFLMIAEENNFYNLVLSMKSSN
                     TQVMVQAYRLLVKQMMEKGMNYPLHLGVTEAGEGEDGRIKSAVGIGTLLEDGLGDTIR
                     VSLTEEPEFEIPVARALVQRYANREKHANIPAINQYPIDPFNYSRRTTLEAGNIGGEN
                     VPRVVMDFSQVSLENPAVLYRVGYAYSVPMDKWNLTDLACDFIFSGTQIPAFELPGTL
                     GVICDESVWTPGKFNLFPLIQSSSYKQGSLQPNQKKFIAVEIQDFDQVLFDKIKLDPS
                     AVLVCSTQNAHAMAELRRIVVYLEEQQIKTPVIIRRSYDLPDLSTFQLAAATDFGGLL
                     IDGLGDGIWLDATNDQPAIRETNQTAFGILQAARVRISRTEYISCPSCGRTLFDLQET
                     TAKIRKRTDHLKGLKIGIMGCIVNGPGEMADADFGYVGTGIGKITLYKGKEVVKRNVP
                     AENAVDELIQLIKDHGKWVDFQA"
     gene            complement(3216..3893)
                     /locus_tag="PK281_04120"
     CDS             complement(3216..3893)
                     /locus_tag="PK281_04120"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY48562.1"
                     /translation="MKYVLFSLLSVCFTLNGTAQSISISNFAMPDTVTLNFSTDVFFH
                     VKNTSDSLNILGNLKLNFLNETTGGPAVPLGGFDAMQFIAPQQERSFEVVVDITPQYF
                     QEGGNTIVIWPSFVGQPINSEDSIRINIYVNNINGIDQEKNQIESAYLIPNPVKGELS
                     ILPIGKAELPESITVRSLNGQILLNQRLNGSGKIDMLKISPGVYILEMSLINGEKITQ
                     RIVRIAE"
     gene            4008..5507
                     /locus_tag="PK281_04125"
     CDS             4008..5507
                     /locus_tag="PK281_04125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48563.1"
                     /translation="MHYSYPLKTRNAMDVLNQIIDGLSKEEIRFFKIFAHRQDSSGER
                     KDMALLDYIRKNPESESESKIVTKLYGKGDKNAYYRLKNRLLEDLNKSLVLQHSSDEE
                     ILQIYQLLAIVRIFTSKNQHQIAFHFLRKAEQRAQKVENNELLDIIYGEFIQLSHTLL
                     VINPEIYIAKRKENNEALARLREMDDVLAVVSYRLKISQNFGEQENALKNMLELVTNQ
                     YAGNATFLRSGRFRIKLFSLVSQLLLQRKDYLSLEDYLVQTWKEFSEEHIFTRNTHDT
                     KLQLLTYIVNALFKNGKVSESLAFAENLHAAMQEFNNLFYDKYEIFYYNALVNNYSTS
                     DIPKAITLLQEMQNRPNLKKVPFYELFIYLNLATSYFDLKNYNQAIKNLNKLYHIDSY
                     PKADKTLRFKISIAELIVRYELNDLDFWKYRYEQISREYQDEFSRPSGQRELDLLKMM
                     DRGVNEVEGLKNKSLRADLDELLAHLKQTSTEDEVIHYYRWLNEKVYGK"
     gene            5497..6636
                     /locus_tag="PK281_04130"
     CDS             5497..6636
                     /locus_tag="PK281_04130"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HOY48564.1"
                     /translation="MENKRVYFDYNASTPVDPRVLESMLPYFTEVFGNASSRAHAFGW
                     EAAEAVAIAREQVANLIGCETNELTFTSGSTESLNLALQGIARMYQSKGKHLITFSTE
                     HKAVLEPLFYLKDLGWEVDVLPVDERGLPNLEHLKKAIRSDTVFVAAMLANNESGIVW
                     PIAEMAELTRASGAFFICDATQACGKIPVDVNELGVDVLALSAHKFYGPKGCGALYLR
                     RKNPRVNLSPLFFGGGHENGKRPGTLNVPGIVGLGKAAELAKESLEHYAQKTSQLRDL
                     FEKELMKLEGVHIHGIDIQRLPNTSAFRIENVPATRLIQGMPNYAFSTGSACSSALPE
                     PSHVWTALGLTEEQAYETVRFSFGMFNQEEEIRNGISILRNIVNG"
     gene            complement(6638..7018)
                     /locus_tag="PK281_04135"
     CDS             complement(6638..7018)
                     /locus_tag="PK281_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48565.1"
                     /translation="MKTALITDQHLEHREWLNRLDFYRDEIKIMQNRIGEIASKNTAQ
                     EVLVQVEHFQNQLLIQSAHIDKLNHDIRKHESEIAAEIAKNPTASDHRRSEVHPEHKE
                     GIEMFEKLFNELRREQILWLAKVM"
     gene            complement(7103..7759)
                     /locus_tag="PK281_04140"
     CDS             complement(7103..7759)
                     /locus_tag="PK281_04140"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HOY48566.1"
                     /translation="MESAIWNGFVMGLGLSFSFGPVFFMLIQTSIDKGWKESLIFDAG
                     VLISDFIIIAAAMMMIFAMGVNVDFSNPAIQFWTTMIGALILIIFGLVLILNPKKETS
                     PTEVPEISTVSKLNHTGLFFKGLGINFLNPSVFLIWFGAVPAVAGSFKGDMSLIIAFF
                     SSTLISYFLIDLGKIYMARKLKRFLTPKAITLFHRISGIIFLAIAVWLLYRFYMRTHS
                     "
     gene            complement(7759..9294)
                     /locus_tag="PK281_04145"
     CDS             complement(7759..9294)
                     /locus_tag="PK281_04145"
                     /inference="COORDINATES: protein motif:HMM:NF015291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HOY48567.1"
                     /translation="MALVGIAIKRALTLQKTLRRVRRRQSPEKKQEKTFLKLIKKAQN
                     TALGRAYGFDEILRADNPYAVFSKQVPIFNYESIHAAWWHRSLEGEEDVAWPGKVQYF
                     ALSSGTSGAPSKKIPVTQAMIRSMRRTSVRQILSLAYYDLPPELFEKGIFMLGGSTDL
                     QWKSSYYEGDLSGINGTQIPFWFQAHYKPGKRIAKERDWEAKLEEIILAAKDWDIGIV
                     VGVPAWIQIVIERIIEHYQVKTIHDIWPNLRIYVHGGVFFEPYKKGFEKLMAHPLHYI
                     ETYLASEGFLAYQGTDKPGSMELVLNNGIFFEFIPFNDSNFDSDGMPLKEAQVLTLEQ
                     VKVGVDYALLISTCAGAWRYMIGDTIRFTDLDNSEIIITGRTKHYLSICGEHLSVDNM
                     NHGIMKAADHFGIGVTEFCVAGEIDETGFRHKWFVGTDDAVDADAFRLKLDEELKAVN
                     DDYIVERRSALKEMHLEILPVSMFYDWMRALGKEGGQNKFPRVLKKEQLAEWESFIQV
                     NKE"
     gene            complement(9308..10105)
                     /locus_tag="PK281_04150"
     CDS             complement(9308..10105)
                     /locus_tag="PK281_04150"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013997866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOY48568.1"
                     /translation="MERPEFLKGKRPTPYRVKKAHVPTLEETDGKIRLNKYISNAGIC
                     SRREADEMIKSGAIKVNGKVVTELGTRVSPEDKIQYGDETLSKETKRYLLLNKPKDTI
                     TTANDPEGRKTVMELVKNACKERLYPVGRLDRTTTGLLLMTNDGELAKKLTHPSYQVK
                     KIYHVSLDKNLKAVDMKRILDGLTLEDGLAEVDEIAYVGEGTDKSEVGIAIHSGKNRI
                     VRRIFEHLGYVVVKLDRTYFAGLTKKDLPRGKFRFLTEKELIMLRMS"
     gene            complement(11266..11628)
                     /locus_tag="PK281_04155"
     CDS             complement(11266..11628)
                     /locus_tag="PK281_04155"
                     /inference="COORDINATES: protein motif:HMM:NF009150.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HOY48569.1"
                     /translation="MALHNDLGKLGENLAVDFLIENGFNILERNWRNRFEEIDIIAIK
                     DSFLVIVEVKMRTNNNYGSPEDAISLRKQRNLVSAAEAYIREKNSDLETRFDVIAVTG
                     TEDQHSIHHIPWAFSPFD"
     assembly_gap    11915..12014
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(12152..13138)
                     /locus_tag="PK281_04160"
     CDS             complement(12152..13138)
                     /locus_tag="PK281_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002976215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3667 domain-containing protein"
                     /protein_id="HOY48570.1"
                     /translation="MSSKHALRKDSICQNCGETVTHRFCSFCGQENTESRQTFRHLLM
                     HFFEDITHYEGKFWKTMKYLFFRPAYLTREFLSGRRVSYVPPVRLYIFISFITFFLPY
                     ILPQYEETNHKPGKSHLTFLADTTIYNSFSFHNDEEGMSLIIPNNFKSQEQLDSLMKH
                     DPYFIIDDEYDYWMNKRFLRMQKYTPLELGEKFADAFSQNFPKALFAYMPLFALVLTL
                     FHRKKGWMYFDHAIFTLHYFSFLLFYVALTRIFDLSEFIVGAEIFGKMNLFVYGSYLV
                     SFPLYFFLAHKKMYSESWIKSIFKSLGIYILNIALFAIVLAVLMFWAVISIH"
     gene            13262..>13563
                     /locus_tag="PK281_04165"
     CDS             13262..>13563
                     /locus_tag="PK281_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019388418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOY48571.1"
                     /translation="MNAPKRYTVTAALPYANGPVHIGHLAGCYIPADTYVRFLRLMGK
                     DVLFICGSDEHGVPITLKARDLGITPQDVVDKYHKLMGDAFADLGISFDIYSRTSAP"
     assembly_gap    13564..13663
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <13664..15145
                     /gene="metG"
                     /locus_tag="PK281_04170"
     CDS             <13664..15145
                     /gene="metG"
                     /locus_tag="PK281_04170"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009411793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HOY48572.1"
                     /translation="KHWFLPLDKYQDDWINDWMLTQKDKWKSNVYGQCKSWLDQGLQP
                     RAITRDLEWGVKVPLEGEEGKVLYVWFDAPIGYISASREWSEKTGKDWKPYWQDEHSQ
                     LVHFIGKDNIVFHCIIFPAILKAHGDYILPDQVPANEFLNLEGEKISTSRNWAVWLHE
                     YLQDFPGRRDELRYVLTSIAPETKDSDFSWKDYQTRVNSELADILGNFVNRVMVLTHK
                     YFEGKVPTAEFNPSAEVEEAATRIFERVPELMLSYEFRAGLIETIGLARAGNKYLADA
                     QPWKLNLDTAREEVEKIIAYSLDIVSRLSVALSPFLPDTSARLRKMLNLPATFEWNDL
                     LISHLPKGHAIGLPEILYKKIEDSEVELQLQKLEASKAARPQPVKAHSPVKPAISYDD
                     FMKMDIRVGTILEAVKVPKADKLLQLLVDTGVDKRTVVSGIAEHFAPEAIIGQQVSLL
                     LNLEPRKIKGIESQGMILMAEDSDGKLKFVQPGEVVNPGSEIR"
BASE COUNT         4231 a         3027 c         3197 g         4509 t
ORIGIN      
        1 ttcctgttaa tgtgagtgat tgagctgtag tgcctgattg tgtcaaacct ttaatacgta
       61 aatcacccac actaaaacca cttcccccaa taccaagcac atttccacta cttaatgttg
      121 aagtacctcc gttttgtcct agtcgaatcc ctaagctggt cccagttgaa ttaaacaaca
      181 aaccgccctg gaatgagttt cccgaagcgg tataggatac ttgaattaaa ccggtgttgc
      241 tagcactaaa tgtagcatcc gacttataag tatcggccga aacattcgaa agaatgacat
      301 tccccgtacc ggtgtttgtc aaactaaacg tagaattata tgtgtttcct ccgttagatg
      361 tattgtcagt agcacttgtt cgagtgatgg ataccggatt attaaatgta ccaccattga
      421 aatacacatt caacatggtg cctgaaattc cacaactcaa tgttgtaccg gcaaatgtgc
      481 aaactgatcc agaaacaaaa gcaatgtttc catttgtaat acttcctcca tcaaatgacg
      541 ctgttccata aatgactaag gtaaatgccg acagatcgag cgaacctgaa gtcatggtga
      601 agttgttcaa tccagatatt tcttcaaata ctggatcgtt tgtttggttg gtaatggtaa
      661 catcatcccc aatatcagga atgcctgcag gtgaccagtt gttgggttcg ccccattggg
      721 tgtcaactga tccggtccac acatagctgg tggcatggag agagactgtc agcacacaca
      781 gtatcgccgg caataaaatt tgcaggggac tcatccggaa aaaatagccg tctcgtttca
      841 taagaaatca gaattatatc tctgaagtct tactatataa acatagcctt agttgcaggt
      901 tttggtaggc tataacatgg tctacttacc gctgcaagaa aaagattgaa atcgaatgaa
      961 ttatcaagta tgaaattttc agatgtctga aattcaggcg tttggcattt ttgaggaagt
     1021 tgactattcc tgtaagaaat gacgaaatgt tcgttttcga tttcaaatca accgcagtgc
     1081 gtactatcct ttcaagttgt tgggagttga cacttatgcc tgaaaatcaa cccatttacc
     1141 gtggtctttg ataagctgaa tcagttcatc cacggcgttt tcggctggaa cgttacgttt
     1201 caccacttct ttgcctttat aaagtgtaat cttaccgatg ccagttccta cgtagccaaa
     1261 gtcggcatct gccatttctc caggtccgtt aacgatacat cccataattc cgattttgag
     1321 tcctttgaga tgatcggttc gcttgcggat tttagcagta gtttcttgaa ggtcgaatag
     1381 tgttctgcca caagaaggac aagagatgta ttcggtacgt gagatacgaa cacgcgctgc
     1441 ttgtaggatg ccgaaagcag tttgattagt ttctcggatt gccggttgat cgttggttgc
     1501 atctagccaa attccatcac ccaatccatc aatcaataaa cctccaaaat cggttgcggc
     1561 agctaattgg aaagtcgaca agtcgggtaa atcgtagctt cgacgaatga tgacgggcgt
     1621 tttaatttgt tgttcttcca gataaacgac tatacgacga agttcggcca ttgcatgtgc
     1681 gttttgtgtg gaacaaacta atacagccga aggatcgagt ttaattttgt cgaataatac
     1741 ttgatcgaaa tcctgaatct caaccgcaat aaatttcttt tggttgggtt gaagacttcc
     1801 ttgtttgtag gaagaagatt ggatgagcgg aaacagatta aatttccccg gtgtccaaac
     1861 cgattcgtca caaataacac ctaaagttcc gggtaattca aaagccggaa tttgagttcc
     1921 actaaagata aaatcacagg caaggtcagt caaattccac ttatccatcg gaactgaata
     1981 cgcatatccg acacgataaa gaacagctgg gttttcaagc gaaacttgac taaaatccat
     2041 caccacgcga ggtacatttt ctccaccaat atttccagct tctagtgtag ttcggcgtga
     2101 ataattgaaa gggtcgattg gatactgatt tatagctgga atattcgcgt gtttctcacg
     2161 atttgcatag cgttgaacga gtgcacgagc aacgggaatt tcaaattcag gctcttcggt
     2221 caaagaaaca cgaatggtat cgcctaatcc atcttcgagt aaagtaccaa ttccaacagc
     2281 cgatttgatt cgtccgtctt cgccttctcc tgcttcggtc acacctaaat ggagtggata
     2341 attcatccct ttctccatca tttgtttcac gagtaaacgg tatgcttgaa ccattacctg
     2401 cgtgttcgac gatttcatgg aaagcactag attgtagaaa ttgttttcct cggctatcat
     2461 gaggaattcc atggccgatt ctaccattcc aagcggacta tcgccatatc gactcataat
     2521 acggtcggaa agcgaaccat gattggttcc gattcgcatg gcagttccgt attctttgca
     2581 gattttcacc agaggagtga agcgttcgcg gatacgatct aattcggctt cgtaggtgct
     2641 atcggtatat tcgatgtgtt cgaacttctt cttatcggcg taattccctg gattcacgcg
     2701 aactttttcc acgatacgag cagcgagttc ggcggcatta ggtgtgaagt gaatatcggc
     2761 aacgagtggt gtgttatagc ctttagctcg caactcacga cgaatgtttt cgagattttg
     2821 agcttctttg atgcttggag cagtaatccg aaccaactcg catcccgcct caatcattcg
     2881 gatactttgc gctacagtag cagcggtatc gagtgtatcg gtggtagtca tcgattgcaa
     2941 tcgaatggga ttatcgcccc caagagccaa atctccaatc atcacttcgc gtgtgcggaa
     3001 gcgttcataa gaagtaaggc tattacaata gattggaatt tggggcagcg taatcatgca
     3061 ggttgttttt tggcaaaagt actcgaacta tacttcgtcc tcaacaggtg atttgcatga
     3121 atagttcaga atagtgtgta aaaaactatt ctggaagaaa ttaataggtt aatgtgagct
     3181 attcacctaa taatagatgt aaaacgagaa cagctttact ccgcaattcg aacaatacgc
     3241 tgggtaattt tctccccatt aatcaagctc atttccaaaa tatatacacc cggtgaaatc
     3301 ttgagcatat ctattttgcc tgatccattt aatcgttgat taagcaagat ttgtccgttt
     3361 aatgatcgaa cggtgatgct ttcaggtaat tcagctttcc caataggaag gatagaaagt
     3421 tcgcccttaa ctggatttgg aattaaataa gccgattcta tttgattctt ttcctgatca
     3481 attccattga tgttattcac gtatatattt atgcgaatag aatcttctga attaattggc
     3541 tgaccaacaa atgagggcca gataacaatc gtattccctc cttcttgaaa gtattgtggt
     3601 gttatatcaa ctactacttc aaacgaacgc tcttgttgag gagcgataaa ttgcatggca
     3661 tcaaatccac ctagtggaac tgctggtcct ccagttgttt cgttaagaaa attcagtttt
     3721 agatttccta aaatatttag tgaatcactt gtgttcttaa catgaaagaa aacatcggtg
     3781 ctgaaattta gcgtaaccgt gtcgggcatt gcaaaattgc taatgcttat cgattgtgct
     3841 gttccattca aggtaaagca cacactcaac aaggaaaata aaacatactt catgaggaag
     3901 ctgaattttt tctcgacagt ttttctctct ggatgtggga gtagcattcg catagccagt
     3961 ttatacaatc aaaagtactt gaatcgaatt cagaataatg gttaaacttg cattattctt
     4021 acccgctcaa gactcgaaac gcaatggatg ttttaaatca gattatagat ggattaagta
     4081 aggaagagat tcgcttcttc aaaatattcg cacatcgtca ggattcctct ggagagcgaa
     4141 aggatatggc cttgttggat tatatccgta aaaatcccga aagtgaatcc gaatcaaaga
     4201 tagttactaa gctctatggc aagggcgata agaatgcata ttatcgccta aagaaccgat
     4261 tgctggagga tttgaacaag tccttggtgc ttcaacattc gtcggatgaa gagattttac
     4321 agatttatca gctcttggcg attgtccgga ttttcacctc taaaaatcag caccagattg
     4381 cttttcattt cttacgcaaa gctgagcaac gagcccaaaa ggttgagaat aacgagttgt
     4441 tggatattat ttatggtgag tttatccagt tatctcacac gcttttagtg atcaaccccg
     4501 aaatatatat tgctaagcgg aaagaaaaca atgaggcatt agcgcgcttg agggaaatgg
     4561 atgatgtgct ggctgttgtt tcttatcgat tgaagatctc tcaaaatttt ggagagcaag
     4621 agaatgcgct gaaaaacatg ctcgaattgg taacgaatca gtatgctggc aatgcaacat
     4681 tcttacgctc cggccgattc cgaattaagt tatttagttt ggttagccag cttctgttac
     4741 agcggaaaga ttacttgagt ttggaggatt acttagtaca aacctggaag gagttttccg
     4801 aagagcacat ttttacccga aacacacacg atactaagct tcaattgctt acctacatag
     4861 taaatgcttt attcaaaaat ggtaaggtga gtgaatcatt agcatttgct gaaaatctgc
     4921 atgctgcaat gcaagagttt aataatctct tctacgataa atatgagatt ttctattaca
     4981 atgctttagt aaacaattat tccacatccg atattcctaa ggctatcacc ttattacaag
     5041 aaatgcagaa ccgtccgaat ttgaagaagg ttcctttcta tgagttattt atttacctga
     5101 atttagctac gagttatttt gatttgaaga attacaatca agccattaaa aatctcaata
     5161 agctctatca tattgatagc tatccaaaag ccgacaagac attacgtttc aaaatttcga
     5221 ttgctgaatt aattgttcgg tatgagttga atgatttaga tttctggaaa tatcgatacg
     5281 agcaaatctc aagggaatat caggatgaat tttcgcgtcc atcgggacaa cgtgaattgg
     5341 atttattgaa aatgatggat cgaggtgtaa atgaggtaga aggattgaag aacaaatcac
     5401 tccgtgccga tttagatgaa ttgcttgcac acttaaaaca aacgagcacc gaagatgaag
     5461 tgattcatta ttatcgctgg ttaaacgaaa aggtttatgg aaaataaacg ggtttatttc
     5521 gactacaatg cgagcactcc cgttgatccg cgagtgttag aaagcatgtt gccctatttc
     5581 accgaagtgt ttggaaacgc atcaagtcgg gcacatgctt ttggttggga agccgctgag
     5641 gctgttgcaa tagcgcgtga acaagttgca aatctgattg gttgcgaaac gaatgaactc
     5701 acatttacat ctggttctac cgagtcgtta aatttagcat tgcaaggaat tgctcgaatg
     5761 tatcagtcga aaggaaagca tttaattact ttttcaacgg agcacaaggc tgtacttgag
     5821 ccactttttt atttgaagga tttaggttgg gaagtggatg ttttaccggt tgatgaacgc
     5881 ggattgccta atctcgaaca tctaaaaaaa gcaattcgtt ctgatactgt ttttgttgca
     5941 gctatgttag ctaataacga aagcggaatc gtttggccta tcgccgaaat ggctgaattg
     6001 accagagctt caggagcatt tttcatttgc gatgcaactc aagcctgcgg caaaatcccg
     6061 gttgatgtaa atgaattggg tgtagatgtg ttagccttat cggctcacaa attttatggt
     6121 ccgaaaggat gtggagcttt atatctaaga cgtaaaaatc cgcgcgtgaa tttatctcca
     6181 ttgttttttg gaggcggaca tgaaaatgga aagcgcccag gtacgttaaa tgttccggga
     6241 attgtagggc taggaaaggc ggctgagtta gctaaagaat cgctggagca ttatgctcaa
     6301 aagactagtc agctaaggga tttatttgag aaagaattaa tgaagctgga aggtgtgcac
     6361 attcatggaa tcgatataca acgattgccc aatacgtctg cttttagaat agagaatgtg
     6421 ccagcaacac ggttaataca aggaatgccg aactatgcct tttcaaccgg atcggcctgt
     6481 tcctctgcct tacccgagcc ctcacatgtg tggacggctt taggtttgac tgaagaacag
     6541 gcctacgaaa cggtcaggtt ctcatttggg atgtttaacc aagaagagga aatccgaaac
     6601 ggaatttcaa tcttgagaaa catcgttaat ggctgaatta catcactttg gctaaccaaa
     6661 gaatttgctc acgacgaagt tcgttgaata gtttttcgaa catttcaatt ccttctttat
     6721 gttctggatg aacttcagag cggcgatggt cagaagcagt tggattttta gcaatctcag
     6781 ctgctatttc tgattcgtgt ttacgaatat cgtgattcaa tttatcgatg tgagccgatt
     6841 gaatcaatag ttgattttga aagtgctcca cttgaactaa aacttcttga gcggtgtttt
     6901 tagaagctat ttcaccaata cgattttgca tgattttaat ctcatcgcga taaaaatcga
     6961 gacgattaag ccattcgcgg tgttctaggt gttgatcggt aataagtgct gttttcataa
     7021 tcgggtcaga ttggttacgg caaaggtgac ctaataaatg agcttgaact atgacctaca
     7081 tcatttagat ggctgatttt tctcagctat gggtgcgcat gtaaaaacgg taaagcaacc
     7141 aaactgcaat tgccaagaag attattcctg agatgcggtg aaatagggta attgctttgg
     7201 gagttaagaa gcgctttagc ttacgcgcca tgtatatttt acccagatca atcaggaagt
     7261 aggagatgag tgtactagaa aaaaaggcga taatcagcga catatcacct ttgaaacttc
     7321 cagcaactgc cggcacagct ccaaaccaaa tcagaaatac cgaaggatta aggaaattga
     7381 tacctaagcc tttgaaaaat aatcctgtat ggttgagttt ggaaacagta gatatttcgg
     7441 gaacttctgt aggactcgtc tctttctttg ggttgagaat caatactaaa ccaaagatga
     7501 taaggatgag tgctccaatc atggtcgtcc aaaactgaat ggctggattg ctaaaatcga
     7561 cattcactcc catggcaaaa atcatcatca tcgccgcagc aataatgatg aagtcgctta
     7621 tgagcacgcc tgcatcgaaa atgagtgatt ctttccaacc tttgtcgata ctggtttgga
     7681 ttaacatgaa aaaaaccggg ccgaacgaaa agctcaggcc aagtcccatg acaaatccat
     7741 tccagatggc gctttccatt attccttgtt aacctgaata aaggattccc attctgctaa
     7801 ttgctctttt ttgagcactc ggggaaattt attttgtccg ccttctttac ctaaagctcg
     7861 catccaatcg tagaacatcg aaacaggtaa aatttcgagg tgcatttctt ttaaagcgga
     7921 acgtcgttca acaatgtaat cgtcgtttac ggctttcagc tcttcgtcga gtttcaaacg
     7981 gaatgcatct gcatctactg catcatcggt tcctacaaac catttatgac ggaatccagt
     8041 ttcgtcaatt tctcccgcca cacaaaattc agtcacacca attccaaagt gatcagcggc
     8101 tttcatgatg ccgtgattca tattgtcaac gctaagatgt tccccacaaa tactgaggta
     8161 gtgtttcgtt cttccagtga tgatgatttc ggagttatct aaatcggtaa atcgaattgt
     8221 atcaccaatc atatatcgcc aagcaccagc acaagtggag atgagtaggg cataatcaac
     8281 accaactttt acctgttcga gcgttagaac ttgagcttct ttcaacggca ttccatctga
     8341 atcgaaattg ctatcgttaa acggaatgaa ttcgaagaaa ataccattgt ttagaaccaa
     8401 ctccatcgaa ccgggcttat cggtgccttg ataggcaagg aatccttctg aagctaaata
     8461 ggtttcgatg tagtggagtg ggtgagccat taatttctcg aatccttttt tataaggttc
     8521 gaagaatact cctccatgca cgtatatgcg aaggtttggc cagatatcgt gaatggtttt
     8581 tacttgataa tgttcaatta tccgttcaat aacaatttga atccaagccg gaacacctac
     8641 aacaatacca atatcccaat ctttggcagc tagaataatt tcttccagtt tggcttccca
     8701 atcgcgttct tttgcaatgc gtttacccgg tttgtaatgt gcctgaaacc aaaatggaat
     8761 ctgggtgcca ttaataccac tcaaatctcc ttcgtaataa ctcgatttcc attgcaagtc
     8821 ggttgatcct cccagcatga aaatcccttt ttcgaagagt tcgggtggaa gatcataata
     8881 ggctaaggaa agtatttgtc gaacgcttgt tctacgcata cttctaatca ttgcctgagt
     8941 aacaggaatc tttttactag gagctcctga agtacctgaa ctaagcgcga aatactgcac
     9001 tttccccggc caagcaacat cttcctctcc ttcgagagat cgatgccacc aagcagcgtg
     9061 gatagattca taattgaaga tgggcacttg tttactgaaa accgcataag gattatcagc
     9121 ccgtaaaatt tcatcaaacc cataagctcg tccaagcgca gtattctgtg ctttcttgat
     9181 cagtttcagg aaagttttct cctgtttctt ctctggactt tggcggcggc gtactctgcg
     9241 aagtgttttc tgcagtgtaa gcgcccgttt gattgcaatt cctactaatg ccatgttcaa
     9301 tcaatactta actcatgcga agcataatca gttctttttc tgtgagaaac cggaatttcc
     9361 cacgtggaag gtcttttttc gtcaagcctg cgaaatacgt gcgatctagc ttaaccacaa
     9421 catacccgag gtgttcaaat atacggcgta caattctgtt tttacctgaa tgaatcgcaa
     9481 taccaacttc agatttgtcc gttccttcgc caacatacgc aatttcatcc acttctgcca
     9541 aaccatcttc taaagttagt ccatccagaa tgcgtttcat gtctaccgcc ttgaggtttt
     9601 tatctaaact cacgtgatag atttttttca cctgataact tggatgagtc agctttttag
     9661 ccaattctcc atcgtttgtc attagcaata aaccagtagt ggttctgtcc aatcggccaa
     9721 ctgggtataa acgttccttg caagcatttt tcaccaattc catcacagtt ttacgtcctt
     9781 ctggatcgtt cgcagtagtg atggtgtctt taggcttatt cagcaataaa taacgcttgg
     9841 tttctttgct gagtgtttcg tcaccatatt ggattttatc ctctggacta acccgagttc
     9901 ccaattcagt aactactttc ccatttactt taatcgctcc acttttgatc atctcatccg
     9961 cttcgcgacg tgagcaaata ccagcattgg agatgtattt attcaaacgg atttttccgt
    10021 cagtttcttc taaagttggg acatgcgctt tcttcacccg ataaggtgtt ggacgctttc
    10081 cttttaagaa ttcaggtcgc tccattggtt cagaccagtc ctgatctgac tttcctcgcg
    10141 gacgaggtgc tgggcgatca cctaattttc ctttgcgttc gaatggacgg tcgccatctt
    10201 tcgcgtattt atcatcgcgt cgtggcggac gatcaccgaa tttcttatcg aatggtttat
    10261 catcgtagcg tttgggacga tcagagtctt ttttgtcaaa aggcttgtca tcgtaacgtt
    10321 ttggactatc accatccttc gcaaatttat catcacgacg tggtggtcga tctccgaatt
    10381 tcttgtcaaa aggtttgtcg tcgtaacgtt ttggacgatc cgaatctttc ttgtcgaaag
    10441 gcttatcatc gtatcgtttc ggacgatctg tgtctttaga gaatttatca tcacgacgag
    10501 gcggacgatc accgaatttc ttgtcggatg gcttatcgtc gtagcgtttt ggacgatcga
    10561 aatctttctt gtcgaaaggc ttatcatcgt aacgtttagg acgatctgtg tctttagaaa
    10621 atttatcatc ccgacgaggc ggtcggtctc cgaatttttt gtcggatggt ttatcgtcgt
    10681 agcgttttgg acggtcgctg ttagaatagc tgtctttgtc tttacgtgga attaaaccat
    10741 ctcgtttttt aggagctgga cgatcggttc cccattcagc attatcgcca cgtttttcgc
    10801 tttcttttac gaaagatttg attgggcgat cacctgaagt ttcaggttta aaagtaactg
    10861 gagggcggtt tccacgcggt tcgcgcttga ccattccttc acgttttttt ccaggaatca
    10921 gtttggcaaa ggcatctaca ccataatctt ctttggaagg tttaggagca gaagctcgtt
    10981 ccggtttttc cgactcaaat gaagcttttg tcgcacgcgg ttttctgata ggtgcatccg
    11041 actccgcttt tggagcacgg gtagcagttt tccttggagc tttctcagct gcaggttcgt
    11101 ctttttttac ccgtgtgcgc cgctcccgcg gggcttcttc gagttctgat tttttgagtc
    11161 gtttcggacg cgtaacacct tcttcgggtt gcactgcgtt ctctgccggt ttcttggcca
    11221 tagggatttc ttgagagcgc aaaagtagcc cattttcccc tataattagt caaacggact
    11281 gaaagcccac ggaatgtgat ggatactgtg ctgatcctca gtgcctgtaa cggcgatcac
    11341 atcgaatcga gtttccaaat cgctgttttt ctcgcggata tatgcttctg ctgcactcac
    11401 cagatttcgt tgctttcgga gactaattgc atcttcgggc gatccgtagt tgttgtttgt
    11461 gcgcattttg acttccacaa tcacgagaaa tgaatctttg attgcgatga tgtcaatttc
    11521 ttcgaatcga ttgcgccagt tgcgttcgag gatattaaat ccgttttcaa tgagaaaatc
    11581 aaccgctaga ttttccccga gttttcctaa atcgttgtgt aatgccattg ggttcatgtg
    11641 tttttgaaac accggaaccg gaactagacg aagttagtaa atgtgctgat gtgttagtgt
    11701 gctggtgtgc tgatttttga tgtgctccct tcgacaggct cagggagcgg aggacgagcg
    11761 ctgagcttgt cgaagcgtgc attagttaat atgctgatgt gtttagtgtg ctgatgtgct
    11821 gatttttgat gtgctccctt cgacaggctc agggagcgaa ggacgagcgc tgagcttgtc
    11881 gaagcgtgca ttagttaata tgctgatgtg ttagnnnnnn nnnnnnnnnn nnnnnnnnnn
    11941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12001 nnnnnnnnnn nnnnaggacg agcgctgagc ttgtcgaagc gtgcattagt taatatgctg
    12061 atgtgttagt gtgctgatgt gctgtttaga tttcgtttaa catgtgccgc gcgctgagct
    12121 tgtcgaagcg ggtggttgag gatttaattt cttaatgaat actaatcact gcccaaaaca
    12181 tcaatactgc taatacaatt gcgaaaagtg ctatgttgag aatgtatatc cccaatgatt
    12241 taaatatgga ttttatccac gattcagaat acattttttt atgagcaagg aagaaataca
    12301 aagggaaaga aactaaataa gagccgtaaa caaatagatt catttttccg aaaatttcag
    12361 ctccaacaat gaattctgac aaatcgaaaa ttcgagtcag agcaacataa aatagcaaaa
    12421 acgaaaaata atgcaaagtg aaaatggcat gatcaaaata catccatcct tttttacgat
    12481 gaaatagcgt caacaccaag gcaaataatg gcatgtaggc aaaaagtgct ttaggaaagt
    12541 tttgagaaaa agcatccgcg aatttttccc ctaattcaag tggagtgtat ttctgcattc
    12601 tcaagaagcg tttattcatc cagtaatcat attcatcgtc gattatgaaa tagggatcat
    12661 gcttcattag ggaatctaat tgttcctgtg atttgaaatt attcggaata attaaactca
    12721 ttccttcttc atcattatga aaagaaaacg aattataaat tgttgtgtct gctaaaaatg
    12781 ttaggtgcga ttttcctggt ttatgattag tttcctcgta ttggggcaaa atatagggta
    12841 ggaaaaatgt gataaaactg atgaaaatgt acaagcgaac aggcggtaca taactgactc
    12901 ttcgtcccga taaaaattca cgcgttaaat aagccggacg aaaaaataaa tacttcatcg
    12961 tcttccaaaa cttcccctcg taatgcgtaa tatcctcgaa aaagtgcatc aacaaatgcc
    13021 gaaaagtttg acggctttca gtgttttctt gtccgcagaa gctgcaaaat cgatgggtaa
    13081 ccgtttcgcc gcagttctga caaatagaat cttttcttaa tgcgtgtttc gaagacatgt
    13141 gtcgaaaata ctcggaatta tagaatgtaa caagtttaag ttggataaga aggtcaaaca
    13201 cgtcgaacga ttgaattctc tgtccattag cgctatcttt gcaccctcaa attcgaatca
    13261 cttgaacgct cctaaacgct acactgtaac tgccgcgctt ccgtatgcta acggaccggt
    13321 tcacattgga caccttgctg gttgctatat tccagccgat acctatgttc gctttttgcg
    13381 tttgatggga aaggatgtcc tttttatttg tggaagcgat gaacatgggg ttccaatcac
    13441 gttaaaagcg cgtgatctag gcattacacc gcaggatgtg gtagataaat accataaatt
    13501 aatgggtgat gcatttgctg atttgggtat ttcattcgat atctattcgc gcacttctgc
    13561 accnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnaaacatt ggtttttgcc
    13681 tttagataaa taccaagatg actggattaa cgactggatg ctcactcaaa aagacaagtg
    13741 gaaatcgaat gtttacggac aatgtaaatc ttggctcgat caaggtttgc agccacgtgc
    13801 gataacgcgc gacttggaat ggggcgtaaa agtaccactc gaaggggaag aaggaaaagt
    13861 gttatatgtg tggtttgatg caccaatcgg gtacatttca gcttcgcgtg aatggtcaga
    13921 gaaaacagga aaagattgga agccatactg gcaagatgag cattctcaat tggtgcattt
    13981 tattggaaaa gacaatatcg ttttccattg catcattttc ccggcaattc taaaagcaca
    14041 cggagattac attttacctg atcaagttcc ggcgaatgaa ttcttgaatc tcgaaggaga
    14101 gaagatttct acctctcgca attgggctgt ttggttgcat gaatatttgc aagactttcc
    14161 aggtcgtcgc gatgagttgc gttatgtgct aacatccatt gctcccgaaa caaaagactc
    14221 agatttctct tggaaagatt atcaaactcg cgttaattct gaactagccg atattttagg
    14281 aaacttcgtt aaccgcgtaa tggtcttaac gcataagtac ttcgaaggca aagttcctac
    14341 agccgaattc aatccatctg ccgaagtaga agaagctgct acacgcattt ttgaacgagt
    14401 tccagaatta atgttgagtt atgaattccg tgctggattg attgaaacca ttggattagc
    14461 tcgcgctgga aataaatacc tagccgatgc gcaaccgtgg aaactcaacc ttgataccgc
    14521 ccgtgaggaa gtagaaaaaa ttattgctta cagcttggat attgtaagtc gtttgtcggt
    14581 tgcattatcg cccttcttac ccgacaccag tgctcgtttg cgcaagatgt tgaatttgcc
    14641 agctacgttc gagtggaacg atttgctgat aagtcatctt ccaaaaggtc atgcgattgg
    14701 attacccgaa attctataca agaaaatcga agattccgaa gtggagctcc aattacaaaa
    14761 attagaagca tccaaagctg cccgcccaca acccgttaaa gcacattcgc ccgtgaaacc
    14821 agctattagc tacgacgatt tcatgaaaat ggacatccgt gttggaacca ttctcgaggc
    14881 cgtaaaagtt cccaaagccg ataaactttt gcagttattg gttgatactg gtgtggataa
    14941 acgtactgtc gtttcgggca ttgcagagca ttttgctccg gaagcgatta ttggtcagca
    15001 agtgagtttg ctactcaatt tagaacctcg taaaatcaaa ggaatcgaaa gtcagggaat
    15061 gattttgatg gccgaagatt ccgatggtaa attgaagttt gttcaaccag gagaagtggt
    15121 aaatccgggt tcggagattc gttgaaataa attacgaatt acga
//
LOCUS       DAOOQW010000041        15122 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_4821_length_15122_cov_5.335900, whole genome shotgun sequence.
ACCESSION   DAOOQW010000041 DAOOQW010000000
VERSION     DAOOQW010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 15122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15122
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4821_length_15122_cov_5.335900"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2217
                     /locus_tag="PK281_04175"
     CDS             <1..2217
                     /locus_tag="PK281_04175"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOY48573.1"
                     /translation="SSTDFPVPGNAFQPTYGGGSQDGMVVKFNANLSTLFWGTYLGGS
                     SPDGVMNLVLDQQGNVFVGGGTTSIDFPTTNGVVQQTNAGGQCDGFITGIRPNGQSIF
                     ASTFYGAELYDQVYILATDRQSNIYVFGQTEKSGNFYQSNFSFSEANGKQFISKFNNS
                     LTTRTWSTSFGNGLAKPDITPSAFTVDICGQIFISGWGGANNSSASGGTFGGTSGLTT
                     TPDAFQTSTDNNDFYLMVLDEADQSLVYATFFGGALSSEHVDGGTSRFDRKGIIYQSV
                     CAGCGGHDDFPTTPNVWSNTNGSQSGCNNAVLKFDFQLPATVASFTSPPIGCAPFTVD
                     FSNSSSFATSYSWLINGTEFSTSENPTSTLTNPGVYTIQLIASNPNSCNAVDTFTKQV
                     RVVNSTHDIFDSLSVCYLSSVELGPPFPVDPYYEVNWNPSIWLNEATAQQPISTPQQS
                     VNYVLYLSLGTCADTIEQYIDVRLSALDAGPDLNICRGQTVSIGLPGDESAYTYLWSP
                     STPLDTQVVAMPLASVDESTMFTLVRSPIDTALGCPGIDSLQINIPPNSPLADFETEV
                     IASCTDVKVKIINNSELADTYNWAFGKGSAPQNDIANPEITYNYGDTIHITLIVNNPN
                     CVDTLEFEQPLKDLSEYFSINHSDSFSPNGDGKNECFSPALQDLPAPDDTRFLSCTSL
                     RIFDRWGITVFESNETPDGCWNGKALNGQDCPSATYFFVFKGQGQELQGSVELIRE"
     gene            2248..6000
                     /locus_tag="PK281_04180"
     CDS             2248..6000
                     /locus_tag="PK281_04180"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOY48574.1"
                     /translation="MKAMQRTLLLFLLVFTSITVWAVDGSVYQRSGFIPNGGQWPSAV
                     KYRIGIQNGALFVRDGGLTYVLYDGEAVKAKHDHSHAGNHETENTSDLIRHHAVQMQL
                     MGANSGIQALTEDGSTTRYNYYQGNIQSKWASGLQAWKKINYPEIYPNTDWIIYTGAT
                     GIKYDFNVKPGGNASLIKMQFTGSESVSIRNNTLYIQTSLGEIIEQAPVAWQEENGVR
                     ELVACSFRQNQDGSIGFDLGQYDHSKLLTIDPQLIFATYSGSTDDNWGYTATYDNAGN
                     GYSGGIVFGANFPTTTGAYQEDFGGGVLDVGILKYNPDGSDALYVTYIGGSSPELPHS
                     MIVNEYDELLIFGTTGSDDFPVTSGAYSSDYKGGPSTSFDGITFNDGLDMFVCRLSND
                     GSQLLGSTLIGGTGNDGLNESLAVNYADQIRGALWVDANNNVYVGTSTQSTDFPTTAN
                     AIQPNYGGGSQDGIVIKLNGNLSQLIWSTYLGGSDADGIFYLVVDTDERAVVTGGTQS
                     SNFPTSASANQGTFGGDKDGFASVIDSAGTTLIASTFIGTGGYDQSFIVGTDKTNHIY
                     LFGQTEATGNTFNVNTAIGVSGGNQFLMKYDPLLTTIVWSNAFGSASGSPDIAPTALL
                     VDLCDKIYCTGWGGVLNPFGQGTNGLITTPDAFQSSTDNNDFYLYVMDNQAQSLIYAS
                     FLGGPNSLDHVDGGTSRFDRKGVIYQSICAGCGGQSDFPGITANSFSPTNNSFNCNNL
                     LVKFDFESPITVSAFASITDPIGCAPYTAEFSNTSVNADLFSWRIGDTEISTASNFTY
                     TFTESGTYEVVLIATSSLTCNAADTVSLTVEVISDIQGTLLPLTACKGDEVTLGPDEF
                     DDPYFQFRWNPGTTLSDSTVRKPTLIADSSLTYSVIVSVGTCVDTISQQLTVYGTERT
                     LLGIAEDCIYDSLEIGPIGEYIPGTTFSWTPATGLSSTSDQNPLAYLIGDRTYTVLIQ
                     RPEGCVDTMDVPVSARWDVMDAGPDVNACSGEPATIGLPDSQGNHTYEWTPTAFLTNE
                     TSATPSATVFQETQFNVIQFPETTSNECPARDSLIVKIVAKPTALFGFELYPDCKGMN
                     VAFSDSSISSEQLLWLFSNGQTSTEENPLTVFPFKDSLVVQLIAKNGECRDTFTFSQY
                     IDDISKYYKENNSNAFSPNNDGINDCFSPALQLVPQPWDIAFVPCSDIFIYNRWGELI
                     FSAEDNDSACWNGKTESGVELPEGVYYYRYRFGSTERAGFVELKRQ"
     gene            5997..6680
                     /locus_tag="PK281_04185"
     CDS             5997..6680
                     /locus_tag="PK281_04185"
                     /inference="COORDINATES: protein motif:HMM:NF014600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HOY48575.1"
                     /translation="MIQLSKEELLGQIDQTTHVGFIQLPIECCDEDAHFLRKEVVEAW
                     LRLFDAAKRDGLELKVISSTRNFERQKQIWENKWTGRTLTNGRNLAEGNLSAEEKARL
                     ILRFSSMPGTSRHHWGSDLDINSLEESYFEEERGKIEYEWLCLNAQKFGFFQPYTAKG
                     TDRNTGYEEEKWHWSFAPVSIPFLDAYLTQIAYSDISGFEGSDTAATLNVIENYVCGI
                     ASPRFSSPI"
     gene            6738..7325
                     /locus_tag="PK281_04190"
     CDS             6738..7325
                     /locus_tag="PK281_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-(ms[2]io[6]A)-hydroxylase"
                     /protein_id="HOY48576.1"
                     /translation="MLRLKKDTDPRWAYLAGQDIEEVLTDHAFCEQKAASTCISLIVK
                     YPEYPELVTEMSALVREEMEHFQRVHEIILKRGMKLGRERKDPYVNELAQFISKEGDQ
                     NKLLVDRLLFSAMIEARSCERFRVLSDNIDDEELAAFYRELMASEAGHYTLFLQFARK
                     YANPNVVDIDVRWQQWLDYEAEVISRYGKIGQIHG"
     assembly_gap    7341..7440
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7518..9365
                     /locus_tag="PK281_04195"
     CDS             7518..9365
                     /locus_tag="PK281_04195"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HOY48577.1"
                     /translation="MKIRVYILLLLCWGSTLVQAGTNKNTITRVDPAFWWVGMQNPQL
                     ELLVYHPGIGAGKVSLSYPQVTLISAVPAENPDFIYITLQIGKEAQAGFVPIDIQLGK
                     KKLHYDYELLARNASQKKAMGVNSSDFIYLIMPDRFANGDPKNDVIAGMNETALTRDS
                     MFYRHGGDLQGIINHLDYLQDLGLTALWINPVQENNEVKTSYHGYAITDHYKIDPRFG
                     DNALYVKLVQELHARGMKMVMDIVPNHIGIHHWLFQNMPSKDWVNQWDNFTKTSYRAP
                     TLLDPYASNFDRKKFNDGWFDQHMPDLNQRNPHVARYLTQNYIWWIEYAGVDDFRIDT
                     YAYSDQAYMAQLSKELHKEYPKLNLFGEIWDHGVGIQAFFTEGFRDRGNFNSGLDGAV
                     DFQLYFALNDALTKPFGWTEGLARVYYVLAQDILYKNANRNVTFLDNHDLSRFYSVVG
                     GDIRKFKMGIAFLMTMRGIPSMYYGTEILMKGFANPDGHVRSDFPGGWASDAKNKFTS
                     QGRTEEENQGFDFVRKLAQWRKANPVVQSGQLMQFVPEESIYVYFRYDDLKTVMVILN
                     ANETAKEIKTERYQERMGRKETGLDIMSGETIQLGTIKLDAWGVKVIEF"
     gene            complement(9433..9828)
                     /locus_tag="PK281_04200"
     CDS             complement(9433..9828)
                     /locus_tag="PK281_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48578.1"
                     /translation="MIKKLLFTFCVIPFGAMAQLSTPDDANLLALHEETPIEVSDEKL
                     KAIDTRLEVGRMDAEYVLYVNSVEQTTMLVQLMDQSGKALPLVRQVNVEEGENLIRLD
                     LTSYPAGAYMISLQAPFQRSSVIHRLYKQ"
     gene            complement(10274..12058)
                     /locus_tag="PK281_04205"
     CDS             complement(10274..12058)
                     /locus_tag="PK281_04205"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48579.1"
                     /translation="MKWTFPFFLYLLFCLNPLLAWETPVLVLPANQALMPTSVLIDWN
                     AVPLSTAYQLEFDTAATFNSPLRQRIQKNYIGSSNSGNDTKHQFNNLYFGEVYYWRVR
                     AWQANDTSQWSATRGFSTLGIVPIQSPANAQLDLNQGSVTLDWYSHPGALQYHLQVDT
                     TNLFNSVSLQNFHKTYTDTSSNAADSRQVINTNLSNHVYFWRVRAINSTDTSAWHLRW
                     FSTGIANIFVPNAPTQLAPEQGLTEQATTQTFQWSSSEFANLYQIYIDTIPQLNNPLI
                     YTIPDTSLTVEILKPNQTYYWYVRAFNTETIVSAWSPLRSFRTYTFIAQPTIVEPLAD
                     AVDVDLNTVFRWDPVPFATHYRFRLATNDSLGGMVEIETSNSQLEFDTLIRKQQYFWS
                     VQAFRDTSFESVASDTISFRLAALHTPQPVFPIHEAQLIDNSSIFSWTPIDEEAQYEL
                     RLSFESDVMHSYVLNTLNTSLEEDSLVWGATYSWNVRAVSDSGNSAWSEMREFLVINP
                     VGIESTKRQEISFWPNPAHSSIQFNRVNTVRQVKIIRSDGVLIRSINDKEIHSELKID
                     LSEFPAGIYSVFIETKEGKWISSRLIKD"
     gene            complement(12411..14675)
                     /locus_tag="PK281_04210"
     CDS             complement(12411..14675)
                     /locus_tag="PK281_04210"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013998019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HOY48580.1"
                     /translation="MAFDIEMIRNVYAGFGARVEAARKVVGRPLTLSEKILYTHLWEN
                     PVTTAFERGKSYVDFAPDRVAMQDATAQMALLQFMQAGRKQVAVPSSVHCDHLITAQV
                     GSSTDLSTAVNSNKEVYDFLASISDKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTD
                     SHTVNAGGLGMIAIGVGGADACDVMAGLAWELKFPKLIGIKLTGKLNGWTSPKDVILK
                     VAGILTVKGGTGAVVEYFGEGAKSLSCTGKGTICNMGAEIGATTSTFGYDDSMERYLR
                     STNRAEVADLANGIREHLTADPEVYANPEKYFDQIIEINLDELEPYLNGPFTPDLATP
                     ISKMKEVAAANGWPMKVDVGLIGSCTNSSYEDISRSVSLARQAIAKDLVSKAEFTITP
                     GSEMVRYTIERDGYLDVFDKLGAKVFANACGPCIGMWNRLGSEKQEKNTIVHSFNRNF
                     AKRADGNPNTYAFVGSPELVTALAIAGDLGFNPLTDTLTNSKGEQVKLDAPSGDELPA
                     RGFAVEDAGYQAPAEDGSSVEVKVSATSDRLQLLDSFPAWEGVDLSGLKLLIKAKGKC
                     TTDHISMAGPWLKFRGHLDNISNNMLIGAVNFFNEKTDAVKNQLTGEYGPVPATQRAY
                     KAAKIGSIVIGDENYGEGSSREHAAMEPRHLGVRAVMVKSFARIHETNLKKQGMLALT
                     FANKEDYDKILENDSIDIKGLTSFAPGKALEIVLHHDNGTSETILANHTYNEAQIEWF
                     KAGSALNLIKAAVK"
BASE COUNT         4534 a         3032 c         3047 g         4409 t
ORIGIN      
        1 tcttcaaccg attttcctgt gcccggaaat gcatttcaac ccacgtatgg tggtggttcg
       61 caagatggga tggtggtgaa atttaatgcg aatttgagca cccttttttg gggaacttat
      121 ttaggtggaa gttctcccga tggtgtcatg aatttagtgc tcgatcaaca aggaaatgtg
      181 tttgttggtg gtggtacaac gtctatcgat ttccctacaa caaatggagt agttcaacaa
      241 acgaatgctg gcggacaatg tgatggattt atcactggaa ttcgtccaaa cggacaaagt
      301 attttcgctt ctacatttta cggagcagaa ctttacgacc aagtttatat actcgcaacc
      361 gaccgtcaga gtaatatcta cgtattcgga cagaccgaaa aaagcgggaa tttttaccaa
      421 tctaacttca gtttttcgga agccaacggc aagcagttca tttcgaaatt caacaacagt
      481 ttaaccacac gaacttggtc aactagcttt ggtaatggtt tggcgaagcc tgatatcacg
      541 ccgagtgcat ttacagtgga tatttgtggg cagattttta tttcgggttg gggaggagcg
      601 aataattctt cagccagcgg tggtacattt ggcggaacta gtggattaac cacaactcca
      661 gatgcatttc aaacctcaac cgacaacaat gatttttacc tgatggtgct cgatgaggca
      721 gatcagagtt tggtttatgc aacatttttc ggtggtgctc tgagttcgga acacgtggat
      781 ggcggaacga gtcgattcga tcggaaagga attatctatc aatcggtttg tgctggatgt
      841 ggcggacacg atgatttccc tactactcca aacgtttggt caaatacgaa tggaagtcaa
      901 agtggttgta ataatgcggt tttaaaattc gattttcaat taccggcaac ggtagcaagt
      961 ttcacttcac cacccattgg ttgtgcgccg tttaccgttg attttagcaa ttcgagcagt
     1021 tttgctactt cgtattcttg gttgataaat ggtacagagt tttcaacttc cgaaaatcct
     1081 acatcaaccc taaccaatcc gggagtttat accattcaat taattgcttc caacccgaat
     1141 agttgcaatg cggttgatac gtttaccaaa caagttcgcg tcgttaattc gacacacgat
     1201 attttcgatt cactttcggt ttgctacttg agttcagtag aattaggacc accatttccg
     1261 gttgatccgt attatgaagt caactggaat ccgagcattt ggctgaacga agcaactgct
     1321 cagcaaccaa tttcaactcc tcaacaaagc gttaattacg ttctttactt atcacttgga
     1381 acctgcgccg atacgattga acaatacatc gatgtgcggc tttcggcttt ggatgccgga
     1441 ccagatttaa atatctgccg cggacaaaca gtttcgattg gacttcctgg tgatgaatct
     1501 gcctatacat atctctggtc gccaagcaca ccgctcgata cacaagttgt ggctatgcca
     1561 ttagcaagtg tggatgaaag tacgatgttc accttggttc gttctccaat tgacaccgct
     1621 ctaggttgtc cgggaattga tagtttacag ataaatattc ctcctaattc acctttagca
     1681 gattttgaaa cggaagtaat tgctagctgt accgatgtta aagtgaaaat catcaataac
     1741 agcgaattag ccgataccta taactgggca tttggaaaag gttctgctcc acaaaacgac
     1801 attgccaatc cggaaattac ctacaattac ggcgatacca ttcacattac tttgatagta
     1861 aacaatccta attgtgtcga cacattggaa tttgaacagc ctttaaagga tttgagtgag
     1921 tatttcagta tcaatcattc cgattctttt tcacccaatg gggatggaaa aaatgagtgt
     1981 ttttcgcctg ccttacaaga tttaccagca cccgatgata ctcgcttttt atcttgtaca
     2041 tcgttgagaa tctttgatcg ttggggaatt accgttttcg aaagcaatga aacacctgat
     2101 ggttgttgga atggtaaagc attgaacgga caggattgtc catcggccac ctatttcttt
     2161 gtgtttaaag gacaaggaca ggaacttcag ggttcggttg agttgattcg agaataagct
     2221 gaatatttgt gtaaattcgt tgcacgtatg aaagctatgc aacgcacact actcctgttt
     2281 ttattggtat tcacaagcat tacagtttgg gctgtagatg gatcggttta tcaacgaagt
     2341 ggatttattc ccaatggtgg acaatggcca tcagcagtaa aatatcgaat tggtattcaa
     2401 aacggagcgc tattcgtgag agatggcggt ttaacgtatg tgttatatga tggtgaagct
     2461 gtaaaagcaa aacatgatca ttcacatgct ggaaatcacg aaacagagaa cacttctgat
     2521 ttaattcgcc atcatgctgt tcaaatgcaa ttgatgggag ctaattctgg tattcaggct
     2581 ctaaccgaag atggttcgac aacccgatac aattactacc aaggtaatat tcaatcaaaa
     2641 tgggcaagtg ggcttcaggc atggaagaaa attaattacc ctgaaatata tccaaacaca
     2701 gactggatca tatacacagg agctactgga attaagtatg attttaacgt aaaacccggt
     2761 ggtaatgctt cactcatcaa gatgcaattt accggttccg aatcggtgag tattcgaaat
     2821 aacaccttgt acattcaaac aagtctggga gaaattatcg agcaagctcc tgttgcttgg
     2881 caagaagaaa atggcgttcg cgaattagta gcttgttcgt ttcgtcagaa tcaagatgga
     2941 agtattggat ttgatttagg tcaatacgat cattcgaaac ttttgacaat cgatcctcaa
     3001 ttaattttcg ccacttattc aggttctaca gatgataatt ggggctacac agctacttac
     3061 gataatgctg gaaatggcta ttcgggagga attgttttcg gagcgaattt tccaactaca
     3121 acaggagctt atcaagagga ttttggcggt ggtgtgttag atgtaggtat tttaaaatac
     3181 aatcccgatg gaagcgatgc attatatgta acatacattg gaggttctag tccagaacta
     3241 ccgcatagta tgatcgtgaa tgagtacgat gaattactca tttttggtac tactggttca
     3301 gatgattttc cagtaacatc tggcgcttat tcatcagatt ataaaggagg accaagtact
     3361 tctttcgatg gaattacatt caatgatgga ttagatatgt ttgtttgtcg cttatcgaat
     3421 gatggcagcc aactattagg ttcaacatta attggcggta ctggcaatga tggtttaaac
     3481 gagtctttag ctgtaaacta tgcggatcaa attcgtggtg cactttgggt agatgcaaac
     3541 aacaatgtgt atgtgggcac aagtactcaa agcaccgatt tccctacaac tgccaatgct
     3601 atacaaccaa attacggtgg aggaagtcag gatggaattg tgattaaatt aaatggaaat
     3661 ctgagtcaac taatttggtc tacatactta ggaggttcag acgctgatgg aattttttat
     3721 ctcgttgtag ataccgacga acgtgcagta gtgacaggtg gaacacaatc gagtaatttc
     3781 cctacttctg cttcagctaa ccaaggaaca tttggtggtg acaaagatgg ctttgcatcg
     3841 gttatcgatt cagcaggaac aactttaata gcatctacat tcattggaac tggtggttac
     3901 gaccaatctt tcattgtggg aactgataag acaaatcata tttatctttt tggtcaaacc
     3961 gaggcaactg gaaatacgtt taatgtaaat accgctattg gtgtaagtgg aggaaatcag
     4021 tttcttatga aatatgatcc tttgcttact accattgttt ggtcgaatgc ttttggctct
     4081 gcatccggtt ctcctgatat cgctcctacg gctttgttgg ttgacttatg cgataaaata
     4141 tactgtactg gttggggcgg tgttttgaat ccgtttggtc aaggtacaaa tggactcatt
     4201 accacacccg atgctttcca aagcagtacc gacaataacg atttctatct ctatgtgatg
     4261 gataatcaag ctcaatcgct tatttatgct tctttcctag gtggcccaaa tagtttagat
     4321 cacgtggatg gtggtacaag tcgattcgat cgtaaaggcg taatttatca atcgatatgt
     4381 gcaggatgtg gcggacaaag tgattttccg ggcattacag caaattcttt ttcacctacc
     4441 aacaactcgt ttaactgtaa taatttattg gtgaaattcg acttcgaatc acctattacg
     4501 gtttcagctt ttgcaagtat taccgatcca attggttgtg caccttatac tgctgaattc
     4561 agcaatacga gtgtaaatgc cgatttattt agttggagaa ttggagatac cgaaatttca
     4621 accgcttcta acttcactta tacatttacc gaaagtggta cgtatgaagt tgtattaatt
     4681 gcgacaagca gtttaacttg taatgcagcc gatactgttt cattaacagt tgaagtgatt
     4741 tcagacattc aaggaactct tctaccatta acagcttgta aaggagatga agttacttta
     4801 ggtccagatg aatttgatga tccatatttt caattccgct ggaatccggg aacaacatta
     4861 agcgattcta ccgttcgaaa acctacactt atagcagatt caagtctaac ctattctgtt
     4921 attgtaagtg ttggaacctg tgtcgacacg attagtcagc aattaactgt ttatggtact
     4981 gaacgaactt tgttaggaat tgctgaagat tgcatttacg attcgcttga gattggtcca
     5041 attggagagt acattccggg aactacattt tcttggacgc cagctacagg cttgagttct
     5101 acttctgatc aaaacccttt agcttacctt attggtgata gaacttatac ggttttaatc
     5161 caacgaccag aaggttgtgt tgatacgatg gatgttcctg ttagtgcccg ttgggatgtg
     5221 atggatgctg gtccagatgt gaatgcatgc tcaggagaac ccgcaactat aggtttaccc
     5281 gattcacaag gaaatcatac ctatgaatgg acgccaacag ctttcttaac aaatgaaacc
     5341 tctgcaactc ctagtgcaac tgtttttcaa gaaacacaat ttaacgtcat tcaatttccc
     5401 gaaacgacta gcaatgaatg tcctgcacgt gattctttga ttgtcaaaat tgtagctaaa
     5461 cctactgctt tgttcggttt tgaattatat cccgattgta aaggaatgaa tgtggctttc
     5521 agtgattcaa gtatttcatc agagcagtta ttatggcttt ttagcaacgg tcaaacaagc
     5581 accgaagaaa atccgctgac tgtatttcca tttaaggatt ctttagttgt tcagcttatt
     5641 gccaagaacg gtgaatgtcg cgatacattt acattttctc agtacatcga cgatatctcg
     5701 aaatactaca aggaaaataa ttccaatgca ttttctccta ataatgatgg aataaacgac
     5761 tgttttagtc cggctttgca gctagttcca caaccttggg atattgcatt tgtgccttgt
     5821 tctgatattt tcatttacaa ccgttggggt gaattaatat tctcggcgga ggataacgac
     5881 tcagcttgct ggaatggtaa aaccgaatcg ggagttgaat tgcccgaagg agtttattat
     5941 taccgttatc gttttggaag caccgaacgt gccggatttg tggagttgaa gcgtcaatga
     6001 tacagcttag caaagaagaa ttattaggtc aaattgacca aacaacacat gtcggcttta
     6061 ttcaactacc gatcgaatgt tgcgatgaag atgcgcattt cttaaggaag gaagtggttg
     6121 aagcatggtt gcgtttgttt gatgcggcga aacgtgatgg cttagagttg aaggtgattt
     6181 catccacccg aaattttgaa agacaaaagc agatttggga gaataaatgg acaggcagaa
     6241 ccttaactaa cggacgaaat ctggctgagg gaaatctatc cgccgaagaa aaagcccgtt
     6301 tgattttgcg cttttcgtct atgcccggaa cgtcgcgcca tcactgggga agcgatttag
     6361 atattaattc tttagaagaa tcgtatttcg aggaagaacg tggaaaaatc gaatatgaat
     6421 ggctttgctt gaatgctcaa aagtttggat tctttcagcc ttatacagcc aaaggaactg
     6481 atcgaaatac gggatatgaa gaagaaaaat ggcactggag ttttgcacct gtttcgattc
     6541 cgtttttaga tgcttatcta acccaaattg cctattcgga tatctcaggg tttgaaggat
     6601 ctgacactgc tgcaactttg aatgtgattg agaactacgt gtgtggaatt gcttctccac
     6661 gattttcatc acccatttaa atacaaccga agcttattta ttctgcttct tgattcaagc
     6721 ttttctaaat ttgcaccatg ctccgattga aaaaagatac cgatcctcgc tgggcatatc
     6781 tagcaggaca agacattgaa gaagtactta ccgaccatgc cttttgcgaa caaaaagctg
     6841 catcaacctg catttcgctg atagtaaaat atccggaata tccagaattg gtaacggaga
     6901 tgagcgcttt agtacgtgaa gaaatggagc attttcaacg ggtacacgaa atcattctga
     6961 aacgtggaat gaaactcggt cgcgaacgca aagatccata tgtaaatgaa ttagcgcagt
     7021 ttattagtaa agaaggtgat caaaataaat tgttggtcga ccgcttactt ttctctgcca
     7081 tgattgaagc tcggagctgt gagcgttttc gtgtgttaag tgataatatt gatgacgagg
     7141 aattagctgc tttttaccgc gaattaatgg cgagtgaagc aggacattat actttattcc
     7201 ttcagtttgc ccgcaaatac gcgaacccaa atgtggtaga tatcgatgtc cgctggcaac
     7261 aatggctcga ttacgaagca gaagtgatta gtcgttacgg gaaaatcgga cagatacacg
     7321 ggtgatttaa ttacgaatta nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7441 attacgaatt gtcaattacg gattatgaat tcttaattgc agattaggga ttggggtttt
     7501 agtgtatttt taacgctatg aaaatccgtg tttacatttt actactgctt tgttggggaa
     7561 gcacgctggt tcaggctggt actaataaga ataccattac acgtgtcgat ccagcatttt
     7621 ggtgggttgg gatgcagaat cctcaactcg aattactcgt gtatcatccg ggaattggtg
     7681 ctggaaaagt atcgctcagc tatccgcagg taacattaat aagtgctgtt cctgccgaaa
     7741 atccagattt tatatacatc accctacaaa ttggaaaaga agcacaagcc ggtttcgttc
     7801 caattgatat tcagttaggg aaaaagaaat tacactacga ttacgaactc cttgctcgca
     7861 atgcttctca gaagaaagca atgggcgtta attcatccga tttcatctac ctcatcatgc
     7921 ccgatcgttt cgcgaatggt gatccgaaaa atgatgtaat agctggaatg aacgaaactg
     7981 cgctcactcg tgattccatg ttttatcgtc acggtggaga tttacaagga attatcaatc
     8041 accttgatta tctgcaagac ctgggtttaa cagccctttg gataaatccc gttcaggaaa
     8101 acaacgaagt caaaacgtcc taccacggat atgcgattac cgatcattac aaaatcgacc
     8161 caagatttgg agataatgca ctatacgtaa aactcgttca ggaacttcat gcacgcggga
     8221 tgaaaatggt gatggatatc gttcctaacc acattggaat tcatcactgg ttgtttcaga
     8281 atatgccttc aaaagattgg gtgaatcaat gggacaactt tacgaaaaca tcctaccgtg
     8341 ctcctactct actcgatccg tatgcatcta atttcgaccg caagaaattc aacgacggat
     8401 ggttcgatca acacatgccc gatttgaatc aacggaatcc acatgtagct agatacctca
     8461 cacaaaacta tatctggtgg attgaatatg caggtgtgga tgatttccgg atagatacct
     8521 atgcatattc tgatcaagct tatatggctc agctttctaa agagctacat aaagaatatc
     8581 ccaagcttaa tttatttgga gaaatctggg atcatggtgt aggcattcaa gcgtttttca
     8641 ctgaaggatt tcgggatcgt gggaatttca attcgggatt agatggtgcc gtcgatttcc
     8701 aattgtattt tgctttgaac gatgcgctaa ccaaaccatt tggttggaca gaaggacttg
     8761 cccgcgtgta ttatgtgcta gcacaagaca tcttgtataa aaatgcgaac cgaaatgtga
     8821 cctttttaga caatcacgat ttaagtcgtt tttattcggt tgtgggaggt gatattcgaa
     8881 aattcaaaat gggaattgct ttcctaatga ccatgcgtgg tattccatcg atgtattatg
     8941 gaacggagat tctcatgaaa ggatttgcta atccggatgg acatgttaga agtgatttcc
     9001 cgggcggatg ggcaagcgat gcaaaaaaca aattcacatc tcaaggtaga accgaagaag
     9061 aaaatcaggg ctttgatttc gttcgtaaac tcgcacaatg gcgcaaagca aatccggttg
     9121 ttcaaagcgg acaattaatg caatttgttc cagaagaaag tatttatgtg tatttccgtt
     9181 acgatgattt aaaaaccgtg atggtcattt tgaatgcgaa cgaaacagca aaagaaatca
     9241 aaaccgaacg ctatcaagaa cgcatgggac gcaaagaaac tggactagat attatgagcg
     9301 gagaaacgat tcaattaggc acgattaaac tcgatgcttg gggtgtaaaa gtgattgaat
     9361 tttaaaatag taaagacata aaaaaagccc cgttagcaaa agttaacggg gctttgcttt
     9421 ttacaaccga ttttactgtt tgtataaacg gtggataaca gacgaacgtt gaaacggtgc
     9481 ttgaagcgaa atcatgtatg ctccggcagg ataagaagtc aaatcgagac gaatcagatt
     9541 ttctccttct tccacattca cttggcgaac caatggcaat gctttgccag attgatccat
     9601 caactgaacc aacatagtag tttgttcgac actgttcacg tataaaacgt attcagcatc
     9661 catacgacct acttcaagtc gtgtgtcgat agctttaagc ttttcatccg acacttcaat
     9721 gggtgtttcc tcatgcaatg ccaacaagtt agcatcatcg ggcgtcgata gctgagccat
     9781 cgcaccaaaa ggaattacac aaaatgtaaa gagtaatttt ttaatcattc aattgatttt
     9841 atgggtttat acaaagctgg ctctttccct acaaattgct tgtcaacata cttgggtgct
     9901 gtcacttatg taagtaaaaa cgagtattcg aataatcatt tattccattt tcaacaatac
     9961 cgtgtattag aaataatatc aaaatttaat tccttaaata atcatgtaat taattagcta
    10021 aatctgattg ttcgacatta aaaataccca taataaaact taaacaataa taaacttcat
    10081 taaatagctt tattcataat taagtattaa aaactctaat gcaaaaatgc acgaaataaa
    10141 atcaaatact aacaaacaaa aatccctcta ttacaggtta atatcgggat taagaaaaaa
    10201 ttgattcttc ttgaaatatg aattcaattt caacaatgaa tcaataatca ttaaaggttc
    10261 attccggttg aaatcaatct tttatcaatc gactcgaaat ccatttaccc tctttcgttt
    10321 caataaatac actgtatatt ccagcaggaa attctgataa atcaattttt aattctgaat
    10381 gaatttcttt atcatttatt gatcgaatta aaactccatc cgaacgtata attttcacct
    10441 gccgaacagt atttactcga ttaaattgaa tactcgaatg ggccggatta ggccaaaaac
    10501 taatttcctg acgttttgta gattcaattc ctaccggatt tattaccaaa aactcgcgca
    10561 tttctgacca agccgaatta cccgaatccg atactgctcg tacattccag gaatatgttg
    10621 ctccccaaac gagcgaatct tcttccaaag atgtattcag tgtatttaac acatacgagt
    10681 gcatcacatc tgattcaaaa ctcaaacgta attcatactg cgcttcttca tcaattggcg
    10741 tccagctgaa tatggacgaa ttatctatta attgagcttc atgaattgga aaaacgggtt
    10801 gaggagtgtg caaagcagct aaacgaaatg aaatggtatc cgaagccaca ctctcaaaag
    10861 aagtatcacg aaatgcctga acactccaaa aatattgctg cttacgaatt aacgtatcaa
    10921 attcaagttg cgaattactc gtttcaattt ccaccattcc acctaaacta tcattcgtcg
    10981 ctaacctaaa tcgataatgc gtagcaaatg gaaccggatc ccaacgaaaa acggtattca
    11041 aatccacatc cacagcatct gccaaaggct ctacaatagt aggttgtgca ataaatgtgt
    11101 atgtcctaaa actccgcaat ggcgaccatg ccgaaacaat cgtttctgta ttaaatgccc
    11161 gtacatacca ataataggtt tgattcggtt ttaatatttc aaccgttaaa ctcgtatccg
    11221 gaatggtgta aataagtgga ttatttaatt gcggaatggt gtcgatatat atctgataca
    11281 aattcgcaaa ttcactcgat gaccattgaa acgtttgtgt ggtagcttgt tccgtcaaac
    11341 cttgttctgg tgctagttgc gtaggtgcat tcggaacaaa aatattagct attccggtcg
    11401 aaaaccaccg taaatgccaa gccgatgtat ctgtcgaatt aattgctctt actcgccaaa
    11461 aataaacgtg gttcgacaaa tttgtattaa ttacttgacg cgaatctgct gcgtttgaac
    11521 tcgtatcagt atatgtttta tgaaaattct gcaacgaaac cgaattaaac aaattcgttg
    11581 tatcaacctg aagatgatac tgtaacgctc cgggatgtga ataccaatct agcgttacgc
    11641 ttccttgatt gagatcgagt tgggcatttg caggcgattg aatcgggaca attcctaaag
    11701 ttgaaaagcc gcgtgtagca gaccattgag aggtatcatt tgcttgccaa gctcgaactc
    11761 gccagtaata aacttctcca aaatacaggt tattaaactg atgtttcgta tcgtttcccg
    11821 aattggaact tccaatgtaa ttcttctgaa tccgttgtcg caagggcgaa ttaaacgttg
    11881 ccgccgtatc aaactctaat tgataagcgg tcgataatgg cacagcattc caatcaatca
    11941 acaccgaagt tggcattaaa gcttgatttg caggcaatac caaaaccggc gtttcccatg
    12001 ccagcaaagg attcaagcaa aataataggt acaagaaaaa cggaaaagtc cacttcatag
    12061 gcgcgcaatc aacgggtact gattcgttca aatgcccaaa aagttgccgc gttttgctgt
    12121 tagaccttac atgaaaggta tttttatgac tatgtgggcg ccaaattacg gtcgtcgcta
    12181 ctcgctgcta tcatcttgct cgcaaggaaa ttaattctaa aacgaaattc cctcaaacct
    12241 caaccatact gctaaacttc cgcgagctca agcaggccgc tccacccgag gcgctttcgt
    12301 gtaaatatta aatccagcaa gaaaaacagc attatcgtct tcaaaaaata gaataaaaaa
    12361 atccctgcca acagctgtga cagggatttc caaataaata atgtgtgaaa ttatttcacc
    12421 gcagctttaa tcaaattcaa ggcagaacct gctttaaacc actcaatttg agcttcgtta
    12481 taagtatggt ttgccaggat tgtttcgctg gttccattgt cgtgatgcaa cacaatttcc
    12541 aacgctttac ccggagcaaa cgaagtcaag cctttgatat cgattgaatc gttctccaga
    12601 atcttgtcgt aatcttcctt gttagcaaag gtcaaagcca acattccttg ttttttcaaa
    12661 ttggtttcat ggatacgagc gaagcttttc accatcaccg cacgaactcc caaatgacga
    12721 ggttccatag cagcatgctc gcgacttgaa ccttcaccat agttttcatc cccaattaca
    12781 atcgaaccaa tttttgcagc cttgtatgca cgttgcgtag ctggaaccgg accgtattcg
    12841 cccgtcaatt gatttttcac tgcgtcggtt ttctcgttaa agaagttcac cgctccaatg
    12901 agcatgttgt tcgaaatatt atccaaatgt ccacggaatt tcaaccaagg accagccatc
    12961 gaaatgtggt cagttgtaca ttttcctttg gctttgatca agagtttcaa accactcaaa
    13021 tcaacacctt cccaagctgg gaaagaatcc aataattgaa gacgatcgct tgtagcagaa
    13081 actttcactt ctacacttga accatcttct gctggagctt ggtaaccggc atcttccact
    13141 gcaaaaccac gtgctggaag ttcatcaccc gatggtgcat ccaatttcac ttgttcgcct
    13201 ttgctgtttg tcaaggtatc ggttaaagga ttaaatccta aatcacctgc aatcgctaga
    13261 gctgtaacta attcaggaga accaacaaat gcatacgtat ttggatttcc atccgcacgt
    13321 ttcgcaaaat ttcggttgaa tgagtgaacg atggtgttct tttcttgctt ttcagaacct
    13381 aaacgattcc acattccgat gcacggaccg caagcatttg caaacacttt tgcccccaac
    13441 ttatcgaata catctaaata accatcacgc tcaatcgtgt aacgaaccat ttcagatccc
    13501 ggcgtaatag taaattcggc tttagaaact aaatctttcg caatcgcctg acgagccaag
    13561 gataccgagc gactaatatc ttcatacgaa gagttggtac atgatccaat caagcctaca
    13621 tccactttca ttggccaacc gttagcagca gcaacttctt tcattttaga aattggtgta
    13681 gccaaatcgg gtgtaaacgg accattgagg tatggttcta attcgtcgag gttgatttcg
    13741 ataatttgat cgaagtattt ttctggattc gcgtaaactt caggatcagc agtaagatgt
    13801 tcgcgtattc cgttagcaag atcagctact tcagcgcgat tggtgctacg taggtaacgc
    13861 tccatggaat catcgtaacc gaaagtagat gtggtagctc caatttcagc tcccatgtta
    13921 cagatagttc cttttcctgt acagctaagt gatttagctc cttcaccaaa atattcaact
    13981 acagcacctg ttccaccttt tacagtaaga ataccagcta cttttagaat aacgtctttc
    14041 ggggaagtcc agccattgag cttacccgtt aattttatac cgataagctt tggaaacttc
    14101 aattcccaag ctaatccagc cattacatca caggcatcgg caccgccaac accaatggca
    14161 atcattccta aaccaccagc attaactgtg tgagaatcgg ttccaatcat catcccaccg
    14221 gggaaagcat aattttctaa aaccacttgg tgaatgatac ccgcacctgg tttccagaat
    14281 ccaattccgt atttatcaga aattgaagca aggaaatcgt aaacttcttt attcgaatta
    14341 acagcagttg aaagatcggt tgaagaacca acttgcgcgg taatcaagtg atcacaatgc
    14401 accgaagaag gcacagctac ttgtttacga ccagcttgca tgaattgcaa taaggccatt
    14461 tgagcagttg catcttgcat ggcaacgcga tctggagcaa aatcaacata ggattttcca
    14521 cgctcgaaag ccgttgttac cggattttcc caaaggtgcg tgtaaagaat tttttcagaa
    14581 agagtaagcg ggcgacccac tactttgcgt gctgcctcaa cacgcgcccc gaaacctgcg
    14641 tacacatttc ggatcatttc gatatcaaat gccatggaat tattttgttt tcgattgata
    14701 gtcagcaact tccaaaattg aaggttgctt tgctaaagta agcgattctg cctacgcaag
    14761 caaggataaa ccggaatgtt tctggaaaat atccatcaaa aaatcgacag taaactagat
    14821 ttaaaaccga agtttaccca tcactcggta cctgcgcgaa tgaaagaaag ggaaacgttt
    14881 atttaatgtg ctaattagct gatgtgctag tgtgctaatg gaaaagaaca ctgctacaaa
    14941 ctccaactaa atcaaacagc acattttttt aatgtgctaa ttagctgatg tgctagtgtg
    15001 ctaatggtaa agaacacagc tacaaactcc aactaaatca aacagcacat caacacacca
    15061 gcacactagc acatttattt atctttgccc gaaagctccc cgcatgtcgc accatcacca
    15121 cg
//
LOCUS       DAOOQW010000042        14471 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_5117_length_14471_cov_6.106964, whole genome shotgun sequence.
ACCESSION   DAOOQW010000042 DAOOQW010000000
VERSION     DAOOQW010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 14471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14471
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5117_length_14471_cov_6.106964"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(80..2242)
                     /locus_tag="PK281_04215"
     CDS             complement(80..2242)
                     /locus_tag="PK281_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013552521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOY48581.1"
                     /translation="MLLLNALEFNLPLKNPVLIFSLILFIVLFVPILLNRVKIPPLIG
                     LIIAGALIGPFGFNLMLRDASVTLFGTVGLLYIMFMAGLEIDLIQFKKNSGKSVILGL
                     YTFIFPMLIGTAVTYYVLHFSLMTSVLLASMFASHTLIVYPLLSKLGVAQNRAVTVTV
                     GATMITDTLALLVLAAVAGMSTGEINNEFWIRLGIGVLLFALIVLFLFPIIGRWFFKI
                     NQESVSQYIFVLGMVFLAGFLAEVAGIEPIIGAFLAGLALNRLIPHTSALMNRIEFVG
                     NALFIPFFLIGIGMLIDYRVFIKDWETLEVAAVMILCAMVSKYLACWLGQKTFGYTAD
                     ERRIMFSLSNAQAAATLATVLVGYNIILGTDASGQPIRLLNEYVLNGTILMILVSCTI
                     ASFAAQKGGRNIALLEAEEQLDGNVLESEKILIPLLSENQIDELIQLSISIKSKKNTK
                     GFYGLSVLGNSPTEVSNEKAAKKILDHAVQSAAATDHTLQPVLRYDSGTSQAIISVIQ
                     EQKISDILLGFTAGEGIEKVFPKELVEEILESSQATLFIYRPTQPLTTIKRQILVVPP
                     NAETEAGFALWLIRVWSLARNTGTKLLVYGHENSLKFFRDLQVKHPVDIQFETFTNWD
                     DFLLFSREVKQDDLFTVIMSRRLHRSYQSAMMRVPGLIEQYFKSNSVLLIFPIQGISS
                     NLNDSGFRDLNMMPSVTENLLRLDEIGKTVLGIFKRNK"
     gene            complement(2486..4582)
                     /locus_tag="PK281_04220"
     CDS             complement(2486..4582)
                     /locus_tag="PK281_04220"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOY48582.1"
                     /translation="MATPRKLLFYLSNQHNLIVKLTIFFISVALMVYVMPREGKFRFE
                     FVKGKVWLHEDFIAPFDFAINKSDSEIREETNLIERAGKKYYVHDSQVLFEKQRDLPA
                     ELDSFIRRVTGVDASAYAPAQKDRLKILENGRDWLSNLYVKGIIQNVLDSPEKSASNL
                     VVEMHDNIAEEKRISDYYTKNAAMDFLATNLYGSNKWEDPELKLFFERFITPNVFYSD
                     QLTQSAREKAISNISLTRGMVSKGELVITKGELVTPEKFQILESVRQEYEAQLGSLQI
                     DSFWVIAGQAGMITILLAMLLLFLILLRRDLFEDSLKLSFLFSLVVAEVVLVSLLTRM
                     HVVSVYIFPFCIMPIIIRSFFDTRLALFAHLLTIMIIGFLVPNPFEFFIIQMTGGIAC
                     IFSIVNMRKRSQLFITITLLTLAYLACYGAITLMVEGQPSAIRYRDMSWLGASAALSL
                     FSYPIIYFIEKVFGFTSDVSLLELSDTNNPLLKQLALQAPGTFQHSLQVANLAEAAIS
                     RIGGNTLLTRTGALYHDIGKMELARYFIENQVTGVNPHDELDFDESARLIISHVKRGI
                     QLARKHSLPEKIIDFIRTHHGNSRVQYFYRSFLRNFPEAEVDEAAFRYPGPIPFSRET
                     AVVMMADAVEATSRSLKIINSDTIDKLVEDIIRQQIEDNQYINANITFKDITAIKKLF
                     KKMLMNIYHVRTEYPK"
     gene            complement(4631..6289)
                     /locus_tag="PK281_04225"
     CDS             complement(4631..6289)
                     /locus_tag="PK281_04225"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOY48583.1"
                     /translation="MFLKQICLFSVLAFIPFFTIWWIRNEWVLFYVGFLNLFPVAITY
                     WFIHKGKLEWASFSSVIFACLNVYMYDDGFGGNTGYYYYLVSTILCGFILFEPNEIRL
                     RTTGIGISLLVLILTNIPGASPRFYNQIEGLDITQFHLYTVNFIVATILTFAVVYYMV
                     AAYRKSEKRLNEALSQAQEVAELKTQFLSNMSHELRTPMNAVVGMTDILIQENPRPDQ
                     LEHLNVLRFSSGNLLHIINDILDYSRIEAGKTEWDNNPFVLRSLLKNLQASLQPQAQA
                     KNIAFSFEIEPNVPGKLSGDENRLIQVLNNLLSNAIKFTHEGKVNLTVRVKSVSPELC
                     KLVFLVRDTGIGIPADKQQVIFDRFTQVSSDMTRQYGGTGLGLAICKRLLEAQNSRIY
                     VSSVLGQGSEFVFALEFNQVHESVVMEKSPSIISDAIRNVRVLLAEDNAINQLIARKF
                     LEGWGVDLKVVNNGREALAHVKANEVDLVLMDLQMPEMDGYEATRSIRRLQGDHFGKL
                     PIIALSASEANEIGEMYSKCGMNDFVHKPFNPEDLFQKMTLHLA"
     gene            complement(6428..7537)
                     /locus_tag="PK281_04230"
     CDS             complement(6428..7537)
                     /locus_tag="PK281_04230"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOY48584.1"
                     /translation="MSHKLRVAVTLSNDLVTDQRVQKVCRSLQSWGHEPILIGRKRKQ
                     SMPLPDWSFKAIRMPLFFETGPAFYAELNIRLFFKLLFISCDAIHANDLDTLLPAFIV
                     SRIRGKQLVYDTHEYFTGVPELVSRPRVQGIWKSIENWIFPKLKHVVTVNQSIADLYE
                     KEYGVKLGVMRNIPSSIPIKPSTRQEMNLPEDKFILILQGNGINVDRGGEEAVQMMEF
                     LDGCLLLIAGSGDVIPALKDYVKIHQLSDKVQFIDRLPYPELMRYTSCCDLGLTLDKD
                     TNINYRFSLPNKVFDYLHAGVPILSSNLPELKRIVEGYDVGKISNQHNPVALAAWVED
                     YKSNLDEQSRLKANCRKAALQLSWENEVQAIRNWY"
     gene            7620..8174
                     /locus_tag="PK281_04235"
     CDS             7620..8174
                     /locus_tag="PK281_04235"
                     /inference="COORDINATES: protein motif:HMM:NF025227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HOY48585.1"
                     /translation="MKSELRSGIAIGISGFLWILLEFFLGFHTVRIDYQPFFNLISFT
                     IPIVGIYWAMKAKRDRYFEGKITFMNALKTGLLITVVMSCISPILNFLYYTVVNPHYQ
                     SIMLSHERDMINGLNLSPEDKNSMLTSSIQNFSLMSLLLKSFLISAITGTLLSLITAA
                     LMKRNNLPDKADVVDHSVSDYTKS"
     gene            8245..9264
                     /gene="murB"
                     /locus_tag="PK281_04240"
     CDS             8245..9264
                     /gene="murB"
                     /locus_tag="PK281_04240"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016650457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HOY48586.1"
                     /translation="MLQLQSTTLRAYNTFGIDVQATRLAEIATSEDILEALEIRQKEN
                     RELLILGGGSNLLFTRDVDDLLLLNRLKGISHSFLSEDEVLVEAAAGENWHTFVQFCI
                     ENNWGGIENLSLIPGNVGASPMQNIGAYGVEIKDVFHSVRAMHVADGSIHEFDAPTCK
                     FGYRESIFKREQKGKFIILSVSYKLTRRNHQLNTTYGAISQELQSQGISNPSIRDVSN
                     AVMAIRRSKLPDPAVLGNAGSFFKNPVVDNSLLESIRLKYPNVVAYPAGDSHSKLAAG
                     WLIETAGWKGYRRDDAGVHALQALVLVNYGSATGAQIWQLSQDIVDDVHSKFGVELER
                     EVNVY"
     gene            9406..11364
                     /locus_tag="PK281_04245"
     CDS             9406..11364
                     /locus_tag="PK281_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009779824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HOY48587.1"
                     /translation="MKGAIAKARAVIERIGPQLDGGQFSVKRIPNESLTVTAHIYGDG
                     HDIVRALLLHRKVGEKKWVEEPFHEVDNDEWSGSFITGKPGMYEYTVVAWIDHALSWH
                     HGFCKKAEAGEKLDLELRIGAELLRKIAKKSKGEDAAWLNTTADLFENEEQYVNAVII
                     AKSDRLEDLIESLPLRENETVGQILPLVVEREKAQFSTWYEFFPRSASPDPARTGTFK
                     DCEALLPLIADWGFDTLYFPPIHPIGVSHRKGKNNSTTAHPGEPGSPWAIGSHLGGHK
                     AINPELGTMADFEKLVKAAKKQGIEIALDIAYQCSPDHPYVKEHPQWFRWRPDGTVQY
                     AENPPKKYQDVLPINFETEDWENLWNELKSVIEFWIGKGINIFRVDNPHTKPFIFWQW
                     IMSEMRRDHPQVIFLSEAFTRPKIMARLAKVGFQQSYSYFTWRQTKAELEEYLTELTQ
                     TELRDYFRGNFWPNTPDILPFYIQKTEHAHSAARYVLAATLSSNYGMYGPVYELLENE
                     PMPGKEEYFNSEKYEARHWDWHTVTPFRELMRKINRIRKENAAFHTTFNIQFCETDNE
                     NLIAYYKISQDKSNQVLTVVNLDPVNKQTGWVKLPETLMPAHEGHTLEMHDLMDDSHY
                     RWNRQWNYVELDPQLISAHIFHITALPH"
     gene            11373..13037
                     /locus_tag="PK281_04250"
     CDS             11373..13037
                     /locus_tag="PK281_04250"
                     /inference="COORDINATES: protein motif:HMM:TIGR02457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_process: GO:0000023 - maltose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative maltokinase"
                     /protein_id="HOY48588.1"
                     /translation="MPESLQRIKDIAQPHIDAEWNDLFNHERCIHQLENQILPVFLKR
                     MRWFGGKARMITAIKIYQNIPLETASSIARYLLLKVRYPDGPTEIYALPVVFIHERFA
                     GLGTFPAEAVIARIGEGNTAGYLIDGISDEDFRNTLFMLMNKRQIQKGDHYPLLASSG
                     KYLVKQGMPSTLKSVVMKADQSNTSVLFNDTFFLKLFRKVEYMINPDWEIVNFLTNQD
                     KFHQIPAYAGSIELQHPEKSPMLLVMLQQLIPNQGDAWKNTLAQLDHFFAQVIEREYH
                     LGPLPLKPHVLNISWDKTPEALQQMIGEEVFHAARLLGKRTAEFHLALIHADNDPAFT
                     PEDLDDVFQQKLLKELMFQVDSKFDLLERNLHRVPFSMRDDATHMVKDKEKVKAFFSK
                     VLEKPLQGKRIRLHGDYHLGQVLVADGDYYLLDFEGEPDKLHSERRDKYPSLKDVAGM
                     MRSYRYAAYATLFQNYQNDPELQAKLIPVADIWYHFVSRYFLGAYIEATQNSGLIPSD
                     DKVNDLLQIYSFQKAIYELGYEINNRPDWALIPLQSLVKFVKHYLD"
     gene            13092..>14471
                     /gene="glgB"
                     /locus_tag="PK281_04255"
     CDS             13092..>14471
                     /gene="glgB"
                     /locus_tag="PK281_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HOY48589.1"
                     /translation="MAKKKDTAEIVEVKPAKKAVSKKSETPAPKSSVKKTAKPAVTKA
                     ETKKASSKIETDQLASSQNLGNGNEDPSSFSLFTDFDIYLFRQGKHYRLYQKLGAHIC
                     DNNGVKGTFFAVWAPNASAVSVIGDFNSWNPEHGHMSARSDGSGIWEVFVPGVGHGSL
                     YKYMLRNAQTGRILEKFDPFAFFNEVPPKRASIVWDLNYSWKAKTWLGQKGRGSASKA
                     WSVYEVHAGSWKKPGSNEDDYLSYNELSKELVAYVKEMGFTHVEFLPLTEHPYSGSWG
                     YQTTGFFAPTSRFGSPQEFMAMVDAFHEAGIGVILDWVPSHFPEDSFALAQFDGTHLF
                     EHQDLRKGFHPDWKSLIFNYGRFEVRSFLISSAMFWADVYKVDGLRVDAVASMLYLDY
                     SRKEGEWEPNIYGGNENLEAISFLKELNETMYYHFPEIQMIAEESTSWPKVSGPVRDG
                     GLGFGMKWMM"
BASE COUNT         4298 a         2947 c         3084 g         4142 t
ORIGIN      
        1 aatacaagta caaatgagaa aattaaactg aatgatttca tccttatttc tggttttatt
       61 tctagtttat tttccaaact tatttatttc gtttgaaaat ccccaaaacg gttttgccaa
      121 tttcatctaa acgaagcaga ttctctgtta cagatggcat catattcaaa tctctaaatc
      181 cagaatcatt caaattagat gaaattccct gtattggaaa gataagtaat acgctgttcg
      241 atttaaaata ttgctcaatt agtcccggaa cgcgcatcat ggctgattga tacgaacgat
      301 gcagcctgcg actcataata accgtaaata aatcatcttg tttgacttca cgcgagaaca
      361 atagaaaatc atcccaattc gtaaaggttt caaattgaat gtcgaccgga tgttttactt
      421 gaagatctcg gaaaaatttg aggctatttt catgtccata aaccaggagt tttgttccag
      481 tatttctagc taaactccat acacgaatga gccataatgc aaagcccgct tcggtttcgg
      541 cattaggtgg gacaaccaat atttgtcgtt taatggttgt taaaggttga gtgggacgat
      601 agataaataa ggtcgcctga ctactttcca atatttcctc cactaattct ttcggaaata
      661 ctttttcaat tccttcacca gctgtaaatc ctaaaaggat atcactaatt ttttgttcct
      721 ggattaccga aattatcgcc tgacttgtac cgctatcata acgaagaaca ggttgcaaag
      781 tatgatcggt tgctgctgca gattggacag catgatcgag tattttcttt gcagcttttt
      841 cgtttgaaac ttctgtagga gagtttccta acactgataa tccatagaaa cctttcgtgt
      901 ttttcttcga tttaatactg atgctgagct gaatcaactc gtcaatttga ttctcggaaa
      961 gaagtggaat gagtattttt tcggattcaa gaacatttcc gtcaagttgt tcttcggctt
     1021 cgagtagggc aatgtttcga ccaccttttt gtgccgcaaa cgaagcaatg gtgcaggaaa
     1081 caagaatcat aagaatggtt ccatttagca catactcgtt gagtaaacga atgggttgtc
     1141 cacttgcatc ggtacctaat atgatgttgt aaccaactaa caccgttgca agcgtggcgg
     1201 ctgcttgtgc attactcaga ctgaacataa tacgacgttc atctgctgta tagccaaatg
     1261 ttttttgtcc taaccagcaa gctaaatatt tagaaaccat agcacaaagt atcatcactg
     1321 cagctacttc taacgtttcc cagtctttaa taaaaacgcg gtaatcgatt aacatcccga
     1381 ttccaatgag gaagaatggg atgaataatg cgtttcccac aaattcaatt cggttcatta
     1441 atgccgatgt gtgcggaata agccgattga gtgctaagcc agcaaggaaa gctccaatga
     1501 ttggttctat gccagcaact tcggcaagaa atcctgcgag gaaaaccatt cccaaaacga
     1561 aaatatattg ggaaacactt tcctgattta ttttaaaaaa ccatcgacca ataatgggaa
     1621 acaggaacaa tacaattaaa gcaaagagta aaactccaat tcctaaccga atccagaatt
     1681 cgttatttat ttcacctgtg gacattccag caacagcagc taagacaagt aatgctaacg
     1741 tgtcggtaat catggtggcg cccaccgtta ctgttactgc gcggttttgt gctactccta
     1801 atttgctgag taatggataa acaatgagcg tatgtgaggc aaacatactc gccaataaaa
     1861 ccgatgtcat gagggaaaaa tgcaacacat aatacgtgac agccgttcca attaacatcg
     1921 ggaaaatgaa ggtgtataat cctaaaatca cactttttcc gctgttcttt ttgaactgaa
     1981 ttaaatcgat ttcgagtcca gccatgaaca tgatatacag caatccaacc gtgccgaaaa
     2041 gcgttacact ggcatcgcgt agcatgagat taaacccaaa tggaccaatg agcgcaccgg
     2101 caatgatgag tccgatgagt ggtggtattt taactcgatt gagcagaatg ggcacaaaaa
     2161 ggactatgaa aagaatcaac gagaaaatga gaaccggatt ctttaatgga agattgaatt
     2221 cgagtgcgtt tagtaaaagc atttgggttg ggttgaacca aaagtaggtt aaacgaagct
     2281 tggaattgat ttattccaat aaaaggttgt tgggttttaa tttatgttga atgatgattt
     2341 taggaataag tctactgatt ttattgataa cgtattatta gtttttactt ctaagcggta
     2401 aggattgaag cgccagcttt cgctaaagct tcagctggtg aaaagaaatt gaagattata
     2461 gccaagctcg accacccaaa aaatattatt taggatattc tgtccgtacg tgatagatgt
     2521 tcatgagcat tttcttgaac agttttttga tggcggtgat gtccttgaaa gtgatgttgg
     2581 cgttgatata ttgattatcc tcgatttgct gacggatgat gtcctcgacc aatttatcga
     2641 tggtgtcgct gttgatgatt ttaaggcttc ttgatgttgc ttccacagca tcagccatca
     2701 taaccacagc ggtttcgcgg gagaatggaa ttgggccagg atagcggaaa gctgcttcat
     2761 cgacttcggc ttcggggaaa ttgcgcaaga aggaacggta gaaatactga acccggctat
     2821 ttccgtggtg tgtgcggatg aaatcgatga ttttttcggg aagtgaatgt ttacgggcaa
     2881 gctgaattcc gcgtttaacg tgcgaaataa ttaagcgagc actttcgtcg aaatcgagct
     2941 catcgtgagg attcacgccc gttacctgat tttcgataaa gtaacgcgcc agttccattt
     3001 ttccaatatc gtgatacaaa gcaccagtcc gggtaagaag cgtatttcca ccaattcggg
     3061 agatggcagc ttcggccaaa tttgctacct gtaaagagtg ctgaaacgtt ccaggagcct
     3121 gcaaagccag ctgttttaga agtggattat tggtgtcgga taattcgagt aatgacacat
     3181 cgctcgtaaa gccaaaaact ttttcgatga aataaataat cgggtaggag aaaagtgaaa
     3241 gtgcagcact ggctcctaac caagacatat cgcggtaacg tatagcagaa ggctgacctt
     3301 cgaccattaa cgtaatagca ccatagcaag ccagatatgc aagcgtgagt aaagtaatgg
     3361 tgatgaataa ttgcgaacgt ttacgcatgt tcacaatgct gaaaatacat gcaataccac
     3421 ctgtcatttg aataatgaaa aactcaaatg gatttggcac caaaaaaccg ataatcatga
     3481 tggtgagcaa atgtgcaaat agcgccaatc gtgtatcgaa aaatgagcga ataatgatgg
     3541 gcatgatgca aaatggaaag atgtaaacac ttaccacatg catgcgtgtg agcagactaa
     3601 caagcaccac ctcagccacc accaaactaa ataggaaact gagtttgagc gagtcttcga
     3661 acaaatcgcg tcgaagcaaa atgaggaata aaagcaacat cgccagtaga atggtgatca
     3721 ttccagcttg tcctgcgatg acccaaaaag agtcgatttg tagggatcct aattgggctt
     3781 cgtattcttg tcggaccgac tcgagaatct gaaatttttc gggagtaaca agttctcctt
     3841 tggtaataac gagttctcct ttactcacca ttcctcgtgt taaggaaata ttgctgatag
     3901 ctttttcgcg agccgattgg gtgagttgat cgctataaaa aacgttaggt gtgatgaagc
     3961 gttcgaagaa gagttttaat tcgggatctt cccatttatt gctaccatag aggttggtag
     4021 cgagaaaatc cattgctgca tttttggtgt aataatccga gatgcgtttt tcttcggcaa
     4081 tgttatcgtg catttcgacc accaaattgg acgcactttt ttcggggctg tcgagtacgt
     4141 tttggatgat gccttttaca tacagattcg ataaccaatc gcgaccattt tcgagaattt
     4201 tcagtcggtc tttttgagct ggagcatacg cgcttgcatc gacgccagta acacgtcgaa
     4261 tgaagctatc tagttcggca ggtaagtcgc gttgtttttc gaataaaacc tgcgaatcgt
     4321 gcacataata tttctttccg gcacgttcga tgagattggt ttcttcacga atttcagaat
     4381 cggatttatt aatagcaaaa tcgaatggtg caataaaatc ttcgtgcagc caaaccttac
     4441 cttttacaaa ttcgaaacgg aattttcctt cgcgcggcat gacgtaaacc atgagcgcaa
     4501 cactgatgaa aaaaatcgtc agtttaacaa tcagattgtg ctgattggat agataaaaga
     4561 gcaatttacg cggagtcgcc atcgccgacg aatatacggc gattcagaaa aagctggatt
     4621 tagaaaatga ttaagctaga tgaagcgtca ttttttgaaa taaatcctct ggattgaatg
     4681 gtttatgcac gaaatcgttc atgccacatt tcgaatacat ttcaccaatt tcgttcgcct
     4741 ccgaagccga aagtgcgatg atgggtaatt tcccaaaatg atcaccttga agtcggcgta
     4801 tcgaccgtgt ggcttcataa ccatccattt cgggcatttg taaatccatc aacactaaat
     4861 caacttcgtt tgctttcacg tgagccaaag cttctcgtcc attattgacc acttttaaat
     4921 caacgcccca accttcgaga aatttgcgag caattagctg gttgattgca ttatcctcgg
     4981 ctaaaagaac acgtacattt cgtatagcat cagaaattat agaaggactt ttttccataa
     5041 ctactgactc atgaacttga ttaaattcta gtgcgaatac gaattcagaa ccttgtccta
     5101 gcacactact tacataaata cgactgtttt gagcctcgag taagcgtttg cagatagcca
     5161 atcctaaacc tgttccaccg tattgacgag tcatatcgga tgaaacttgg gtaaatcgat
     5221 cgaaaatgac ttgttgttta tctgcaggaa ttccaattcc cgtatcgcga accagaaaca
     5281 cgagtttgca caattcagga gaaaccgatt ttactcgtac agtcaagttg acttttcctt
     5341 cgtgagtgaa tttaatcgcg ttgctgagca agttatttaa tacctgaata agtcggtttt
     5401 catctcccga aagtttccct ggaacgtttg gttcaatttc gaaactaaag gcaatgtttt
     5461 ttgcttgagc ttgaggttgt agggatgctt gtagattttt aagtaatgaa cgaaggacaa
     5521 atggattatt atcccattcg gttttacccg cttcgatgcg agaatagtcg agaatatcgt
     5581 tgatgatgtg aagtagattt ccagaggaaa atctcagcac gttaagatgt tccagttgat
     5641 cggggcgagg attttcttga attaaaatat cggtcattcc tactacagca ttcatgggcg
     5701 tacgcaattc gtggctcatg ttggaaagga actgcgtttt tagttcagcc acttcttgag
     5761 cttgactaag tgcttcgtta agtctttttt ctgattttcg atacgctgca accatgtagt
     5821 aaaccactgc gaatgtgagt atggtagcaa caatgaaatt tactgtatac aagtgaaatt
     5881 gagtgatgtc gagtccttca atttgattgt aaaaacgagg tgatgcaccg ggaatattcg
     5941 ttaggattaa gactaacaag gaaattccaa ttccagtagt tcgaagtcga atttcattgg
     6001 gttcgaacaa gataaatcca cataaaatgg ttgatacgag gtaataataa taaccggtgt
     6061 taccgccaaa gccatcatca tacatataca cgttaagaca agcaaaaatg acagaactaa
     6121 atgaagccca ctcaagcttt cctttgtgta taaaccaata cgtgatagct acaggaaata
     6181 aattgagaaa gcctacataa aatagaaccc actcatttct aatccaccaa atcgtaaaaa
     6241 acggaataaa agctagtacc gaaaacaaac agatttgttt caggaacatg ttgcggtttt
     6301 caagcatagt cgtgttgcat gtgcggttgt attacgcttt attgatagcg ctgttatact
     6361 gcaaacacgc gagatgacgt actaccttac atacgtaaca gttgacatca cggtacgaaa
     6421 gtgttgctta ataccaattt cgaatggctt gcacttcatt ctcccagctc agttgtagcg
     6481 cggctttccg acaatttgcc tttaatcggc tttgttcgtc caaatttgac ttgtaatctt
     6541 cgacccatgc tgctaaagca accggattat gctgattgct gatttttccc acatcgtaac
     6601 cttccacaat acgtttgagt tcgggcaagt tgctcgataa aattgggaca cctgcgtgca
     6661 aataatcaaa cactttattc ggtaaactaa atcgataatt aatattcgtg tctttatcga
     6721 gtgtaagtcc taagtcgcaa cagcttgtgt atcgcattaa ttcaggatat ggcagacgat
     6781 cgatgaattg tactttatca gagagttgat gaattttcac gtaatctttt aatgcaggaa
     6841 ttacatcacc agaaccagca attaataaaa gacaaccatc cagaaattcc atcatttgaa
     6901 cagcttcttc tccaccacga tctacattta ttccgttgcc ttgaaggatg agaataaatt
     6961 tgtcctcagg taagttcatt tcctggcgag tagatggttt aatgggaatt gaggaaggaa
     7021 tatttcgcat aactcccaat ttcacaccat attctttttc gtataaatcg gctatagatt
     7081 gatttaccgt aacgacgtgt tttaatttag gaaaaatcca attttcgatg cttttccaaa
     7141 tgccttgaac tcttggacgt gaaacgagtt ccggcacacc tgtgaaatat tcatgcgtgt
     7201 cgtacaccag ttgttttcct cggattcggg aaactataaa agcgggcaat aatgtgtcta
     7261 gatcgtttgc atgaatcgca tcgcaagaaa taaacagcag tttgaagaat agtcgaatat
     7321 tcagctcggc gtaaaaagct ggacctgttt cgaaaaacaa aggcatacga atcgctttga
     7381 acgaccagtc gggaagtggc atactttgtt tacgcttccg gcctatgaga atcggttcgt
     7441 gtccccaact ctgtaacgaa cgacaaactt tctgcacccg ttgatcggtt acaaggtcat
     7501 tacttaaggt tacggcaaca cgaagtttgt gcgacattcg gcgaagttaa acaaggcaat
     7561 cgtgcaggca agtcggattt tgtacctttc gcactttaac gaattggaat aggatggaaa
     7621 tgaagtcaga attaaggtcg ggaattgcga ttggcataag tggatttctt tggattttac
     7681 ttgaattctt tttgggattt cacaccgttc gtatcgatta tcagcccttt ttcaacctta
     7741 tttcattcac cataccaatc gttggaattt attgggcgat gaaagccaaa cgtgatcgct
     7801 atttcgaagg aaaaatcacg ttcatgaatg cactaaaaac gggtttgctc attacagttg
     7861 tgatgagttg tatcagtcca attctgaact ttctctatta cacagtggtc aatccgcatt
     7921 atcagagcat catgttaagt cacgaacgcg atatgattaa cggactcaac ctttcgccgg
     7981 aggataaaaa ttccatgctt accagttcga ttcagaattt ttccttgatg tcattgctct
     8041 tgaaatcgtt cctaatatcc gccattacgg gtactttact tagtttaatc accgctgctt
     8101 tgatgaaacg aaataatttg cctgataaag cagatgttgt tgatcattcg gtgagcgatt
     8161 acaccaagtc atgagcatgt tggctgttga aaaatagctg cattcggttt agtttgcttg
     8221 ttgcgatgaa ttagcttcgc ttcgatgtta cagcttcaat ctactacttt gcgcgcatac
     8281 aatacgtttg gtattgatgt tcaagctacg cgtttagccg aaattgctac ttctgaggat
     8341 atattagaag cactcgaaat tcgccagaaa gagaatcgtg aattattgat tttaggtggc
     8401 ggatcaaacc tattatttac tcgtgatgta gatgatttgt tattactcaa ccgcttgaaa
     8461 ggaattagtc atagtttcct gtcggaagat gaggttttag ttgaagcagc agcaggagaa
     8521 aactggcata cgttcgttca attctgcatt gagaacaatt ggggcggaat cgaaaattta
     8581 tcactcattc ccggcaatgt tggcgcttct cccatgcaga atataggagc gtatggagta
     8641 gaaattaaag atgtatttca ttctgttcgt gccatgcatg tagccgatgg aagcattcat
     8701 gaatttgacg ccccaacgtg taagtttgga taccgagaaa gtattttcaa acgggagcaa
     8761 aaggggaaat ttatcatcct gtcggtgagc tacaaactta ctcgtcgcaa tcatcagctc
     8821 aatacaacat atggtgctat ttcccaagag ctccaatctc aaggaatatc caatccaagt
     8881 attcgcgatg taagtaatgc cgtaatggcg attcgtcgtt ctaaactacc cgatccagct
     8941 gtcttaggaa atgccggtag ctttttcaaa aatccggtag tcgataattc cctgctagaa
     9001 tccattcgcc tgaaataccc gaatgtagta gcctatcctg ccggcgattc gcattcgaaa
     9061 cttgccgccg gttggcttat cgaaactgct ggttggaaag gatatcgccg cgatgatgct
     9121 ggtgttcacg cccttcaagc acttgttctc gttaactacg gctctgccac cggtgcccaa
     9181 atctggcagc tctcccaaga tatcgtcgac gatgttcact ccaaattcgg tgtcgaactc
     9241 gaacgtgaag ttaacgtgta ttaaaaatcc cccccttcga caagctcagg ggcgggattg
     9301 ttgtcttcgc tccctgagcc tgtcgaaggg agcacatcat ctccttagtg tcatttttac
     9361 aacatttttc ttgactcatc catctcatct cctatatttg gatgtatgaa aggagcaata
     9421 gccaaagcac gggcagttat tgaacgtatc ggtccgcaac tagatggagg acaattctca
     9481 gttaaacgca ttccaaatga gtcgcttaca gttacggctc atatttatgg agatgggcat
     9541 gacatcgttc gggcattatt acttcatcgt aaagtggggg agaaaaagtg ggtggaagaa
     9601 ccctttcacg aggtggacaa cgatgaatgg tcgggtagtt tcattacggg aaagcccggt
     9661 atgtatgaat atactgttgt agcatggatt gatcatgcat taagttggca tcacggattt
     9721 tgtaagaagg ctgaagcagg ggagaagctc gatttagaat tacggatagg agcagagctc
     9781 ttgcggaaga ttgctaagaa atcgaaaggg gaagatgctg cttggctcaa cactacagcc
     9841 gatcttttcg agaatgaaga acagtatgta aatgctgtaa tcattgcgaa aagcgatcgt
     9901 ttggaagatc taattgaatc cttaccctta cgtgaaaatg aaacggttgg acagattcta
     9961 cctttagttg tggagcgtga aaaggcgcag tttagcactt ggtatgagtt ttttcctcgt
    10021 tcggcttcac ccgatccggc acggactggc accttcaaag attgtgaagc attattgcca
    10081 ctaatcgccg attggggttt tgatacactc tattttcctc ccattcatcc cattggtgtg
    10141 tcgcatcgaa aagggaaaaa taacagcact accgcacatc ccggtgaacc tggttcgcct
    10201 tgggcaattg gttctcatct tggcggacat aaagccatca accccgaatt gggcaccatg
    10261 gccgattttg aaaagctcgt gaaagctgcc aagaaacagg gcatcgaaat cgctttggat
    10321 attgcctatc aatgttcgcc cgatcatcca tacgtaaaag aacatccaca atggtttcgt
    10381 tggcgccccg atggtacagt tcagtatgcc gaaaatccgc ctaagaaata ccaagacgtt
    10441 ttgcccatca acttcgaaac agaggattgg gaaaatcttt ggaacgaact gaaaagtgta
    10501 attgaattct ggattggcaa gggaatcaac atttttcggg ttgacaatcc gcacacgaaa
    10561 ccatttattt tctggcagtg gataatgtca gaaatgcgac gcgatcaccc acaagtgata
    10621 ttcttaagcg aagctttcac ccgtcccaaa atcatggctc gtttggcaaa agttgggttt
    10681 caacagtcat actcgtattt cacgtggcga caaacaaaag ccgaactcga agaatatctg
    10741 accgaattaa cccaaaccga attgcgggat tatttcagag gaaatttctg gcccaataca
    10801 cccgatattc ttccgtttta cattcaaaaa acagaacatg ctcattcagc tgctcggtat
    10861 gtgttagctg ctacgctttc ttcaaattac ggcatgtacg gaccagttta cgaattactc
    10921 gaaaatgaac cgatgcccgg gaaagaagag tattttaact ccgagaaata tgaagcacga
    10981 cattgggact ggcacacagt cacacctttc cgagaattga tgcgaaagat caatcggata
    11041 cggaaagaaa atgcagcctt tcatactacc tttaacattc aattctgcga aaccgacaac
    11101 gaaaatttaa tcgcgtatta caagattagt caggacaaaa gcaaccaagt tctcacggtt
    11161 gtgaatcttg atccagtaaa taagcaaacg ggttgggtaa aactacccga aacgcttatg
    11221 ccggctcacg aaggtcacac acttgaaatg cacgatttaa tggacgatag tcactaccgc
    11281 tggaatcgac aatggaatta tgttgaactg gatccgcaac tgatatctgc tcatattttt
    11341 catatcactg ctcttccgca ttaattaagc atatgcctga atcacttcaa cgaatcaaag
    11401 acattgctca gcctcacatc gacgcagagt ggaacgacct tttcaatcat gagcgatgta
    11461 ttcatcagct cgaaaatcag attcttccgg tttttctgaa acgaatgcgc tggtttggtg
    11521 gaaaggctcg catgattact gccataaaaa tctatcaaaa tattcccctc gaaacagctt
    11581 cgagtattgc tcgctacctg ttgctaaaag tgcgctatcc cgatggtcca actgagattt
    11641 atgctttacc agttgtattt atccatgagc gatttgctgg cttgggaact tttcctgccg
    11701 aagctgtaat tgctcgtatt ggagaaggaa acactgccgg gtatttgatt gatggaatat
    11761 ccgatgagga tttccgcaat accttgttta tgctcatgaa caaacgccaa attcagaaag
    11821 gagatcatta tcctttattg gctagttcgg gtaaatacct agttaaacaa ggaatgcctt
    11881 caaccttgaa atcggttgtg atgaaggctg atcagagtaa cacttcggtc ttattcaacg
    11941 atacgttttt cctcaaactc ttccggaagg tcgaatacat gattaatccc gactgggaaa
    12001 tcgtgaattt cctcaccaac caagataagt ttcatcagat tccagcttat gcgggttcaa
    12061 tcgaattaca acatcccgaa aaatcaccca tgctgttggt tatgttgcag caacttattc
    12121 ccaaccaggg tgatgcgtgg aaaaatacgt tagctcaact cgatcatttt ttcgcacaag
    12181 taatcgagcg ggaatatcat ttaggtccat tgccacttaa gcctcacgtg ctgaatattt
    12241 cttgggataa aacacccgaa gcgctgcagc aaatgattgg ggaagaggtt tttcatgctg
    12301 ctcgtttgct tgggaaaaga actgctgagt tccatcttgc cttgatacat gccgacaatg
    12361 atccagcatt tacgcccgaa gatctggatg acgtatttca gcaaaagctc ttaaaagagt
    12421 tgatgtttca agtggatagc aaattcgatc ttttggaacg aaacctacat cgagtgccat
    12481 tttcgatgcg cgacgatgct acacacatgg ttaaggacaa agagaaagta aaagcattct
    12541 tcagcaaagt cctcgaaaag ccattgcagg gaaaacgaat tcgtcttcat ggcgattatc
    12601 acctcggaca agttttagtg gccgatggtg attattattt gctcgatttt gaaggtgaac
    12661 ccgataaact tcattccgaa cgacgtgata aatatccatc cttaaaagat gtggctggaa
    12721 tgatgcgttc ctatcgttat gcagcctatg caacgttgtt tcagaactat caaaacgacc
    12781 ctgagcttca agctaaactc attcctgttg ctgatatctg gtatcatttc gtaagtcgct
    12841 acttcttagg tgcctatatc gaagcgactc aaaacagtgg tctcattcct tcggatgata
    12901 aagtgaatga tctacttcag atttattcgt tccaaaaggc gatttatgaa ttaggttatg
    12961 aaataaataa tcgtcccgac tgggcattaa ttccgcttca aagccttgtc aaatttgtca
    13021 aacactacct cgattaatca agctgattaa cgaaaaaata ttgttcatca taattagaac
    13081 atctaactcg catggcgaaa aagaaagata ctgccgaaat cgtagaagtg aaaccagcta
    13141 agaaagctgt cagtaaaaaa agcgaaacac cagcacctaa atcatcggtt aagaaaacag
    13201 ccaaaccagc tgtgactaaa gccgaaacaa agaaagcaag cagtaaaata gaaaccgatc
    13261 aattggcgag ttctcaaaac ttgggaaatg gaaatgaaga tccatcgagc ttttcactct
    13321 tcaccgattt tgatatttac ctcttccgcc aaggaaaaca ctatcgcctc taccaaaaac
    13381 taggagcgca tatttgcgac aacaacggtg ttaaaggaac tttctttgcc gtttgggctc
    13441 ctaatgcttc agcggtaagc gttatcggtg attttaatag ctggaatcca gagcatggtc
    13501 acatgtcggc acggagtgat ggttcgggta tttgggaggt ttttgttccc ggtgtgggac
    13561 atggtagctt gtacaaatac atgttacgaa atgcacaaac ggggcgcatt ctagagaaat
    13621 tcgatccatt cgcatttttc aatgaagtgc caccaaaacg tgcgtccatt gtttgggatt
    13681 tgaattattc ttggaaagca aaaacgtggt tgggacaaaa aggtcgcggt tctgcatcca
    13741 aggcttggtc ggtgtatgaa gttcatgctg gttcttggaa aaagcccggt tctaacgaag
    13801 acgattacct aagctacaat gaattaagta aagaacttgt cgcgtacgtg aaagaaatgg
    13861 gctttacgca cgtagaattc ttaccactca ccgaacatcc ctattcagga tcttggggat
    13921 atcaaactac cggattcttc gcaccaactt ctcgatttgg ctcaccacag gagtttatgg
    13981 cgatggtaga tgcttttcac gaagctggaa ttggcgtaat cctcgattgg gttccatccc
    14041 attttccaga ggatagtttt gccctcgcgc aattcgacgg cacgcatctt ttcgaacatc
    14101 aggatttacg taaaggcttt catcccgatt ggaagagcct cattttcaat tacggacgat
    14161 ttgaagttcg ttccttcctc atcagctccg caatgttttg ggccgatgtg tataaagtag
    14221 atggtttacg ggttgatgcc gttgcttcga tgctctatct cgattattcc cgtaaggaag
    14281 gcgagtggga accgaatatt tacggcggaa acgaaaacct cgaagccatt tccttcctaa
    14341 aggaattgaa tgaaacgatg tattatcatt tccctgaaat ccagatgatt gccgaagaat
    14401 cgacttcctg gccaaaagtt tcaggtccgg tgagagatgg tggtttaggt ttcgggatga
    14461 aatggatgat g
//
LOCUS       DAOOQW010000043        13749 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_5469_length_13749_cov_6.135096, whole genome shotgun sequence.
ACCESSION   DAOOQW010000043 DAOOQW010000000
VERSION     DAOOQW010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 13749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13749
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5469_length_13749_cov_6.135096"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1234)
                     /locus_tag="PK281_04260"
     CDS             complement(<1..1234)
                     /locus_tag="PK281_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48590.1"
                     /translation="MKNFIASVLLLFIATVAANAQGSNTSCSNASPFCTGTQFNFPAG
                     TSGEAAEAGPDYGCLGSEPNPSWYFLQIENTGTINIHMVGTNDTIDNTPTNDIDFICY
                     GPFTSLVDVCSQLTADNTVDCSYSGNEEEDVNIPNGITGEYYMILITNFSQEPCNIIF
                     SQNSGSGSTSCGPFNNGPLCAGQDLELVSFFNEVEYAFSWTGPNGFTSTEANPVIPNI
                     TEDMAGVYTLVTTNPTETFTGTTTVEISPSPNPSGFIALGDSCTGGSLTFTPDSVFSN
                     AIYTWTYPDGSTHTGNPYIDNNINPSMDGGISLSYEIDGCFSPPVTEFVKVNPNPQPV
                     IEGDNHSCFDGLVQIGLTEVYDSYTWSNSSTQPINFVNQGTFTVTVIDSNGCTGVSAP
                     FTVTNSQPDAQFTGITAFC"
     gene            complement(1263..1634)
                     /locus_tag="PK281_04265"
     CDS             complement(1263..1634)
                     /locus_tag="PK281_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48591.1"
                     /translation="MKKIALYFFCLTGVCISQSLVAQTITNQETVKPTEVILKKTSEN
                     KHRIFFSLNMQSLNEAQRSAFVNQLYSSKILAVISRISESGELKISCSKVFTISDAEQ
                     EINRIQQAIAQLPVNAKTDKY"
     gene            complement(1716..2705)
                     /locus_tag="PK281_04270"
     CDS             complement(1716..2705)
                     /locus_tag="PK281_04270"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOY48592.1"
                     /translation="MTLEPKVSVIIPTYNYAHFIGETLVSIESQRYQNWECIVVDNGS
                     TDNTKETVALWEQKDSRFKYLYISHTTTSGCRNAGVAKSIGDFIQFVDADDQIAPGKI
                     ENQIALFDKNPDASIVYSNARYYDHGIPDVLRFSHDPTQNKPWMEEYSGRTWDLLAKM
                     FDRNIFVISSPLLKRCVLEDTGGFFEGLNWVEDWDFYFRCFALNYRLVYDPAPDSLSL
                     IRVHPKSLSRNKAMMMEQSLIARTHVKTTLSKLTDYKNGNQLIGENNRYVKFLHRMLV
                     NEYAQNDAFKSRSHLIQFAWLDNDYKLIIKALQSCLTGKTITFPDETNESKPK"
     gene            complement(2722..3285)
                     /locus_tag="PK281_04275"
     CDS             complement(2722..3285)
                     /locus_tag="PK281_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48593.1"
                     /translation="MRFIPYALAILIAITAFSCKKLDQLVSFNLNTHDKVFWETAYDT
                     VLTDTIVGNEYASFMSAEYSFKDYDKFETNKSTPQSVESVEAFTLTILIDSGATHFGF
                     ADSMTVYLSSPKNLFKEVEIAHFTELPTTEHFMAIDMLVPNDDWASIIQKEKYQFRTE
                     FKLLSPMPDSIYLDYSMEFRLKAIPND"
     gene            complement(3336..4049)
                     /locus_tag="PK281_04280"
     CDS             complement(3336..4049)
                     /locus_tag="PK281_04280"
                     /inference="COORDINATES: protein motif:HMM:NF039186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene cyclase domain-containing protein"
                     /protein_id="HOY48594.1"
                     /translation="MPDKFLYLALMIGSLAYPLAQSFERRIELWKKWKHMFPAILLTA
                     GFFLIWDEFFTRIGVWSFSPDYTLGINIGSLPLEEWLFFLIVPYCCFFVYFVTAYFFR
                     KDVLGPFQRIIATTVSLILLTSAIAFYDRWYTAYTCFLPALLLLYLTYVKRVKYLGRF
                     FVGYFFCLIPFLIVNGVLTSKPVVMYNDAENSGVRIFIQGISNIPIEDLAYCMLLLLL
                     NVALYESFAKRNQREIDTR"
     gene            complement(4039..4497)
                     /locus_tag="PK281_04285"
     CDS             complement(4039..4497)
                     /locus_tag="PK281_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004570977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotene hydroxylase"
                     /protein_id="HOY48595.1"
                     /translation="MNWLINAGIIVATFFFMEFMAWATHKYVMHGFLWILHRDHHEPH
                     SNIFERNDAFFLIFAIPSWLCIMFGMMNGNDFRLYIGIGIAVYGFAYFLVHEVFIHQR
                     LNWFKRSDNWYLRAIRKAHKVHHKTLGKEHSESFGMLIVARKFFQEQRAR"
     gene            complement(4501..5148)
                     /locus_tag="PK281_04290"
     CDS             complement(4501..5148)
                     /locus_tag="PK281_04290"
                     /inference="COORDINATES: protein motif:HMM:NF016155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid biosynthesis protein"
                     /protein_id="HOY48596.1"
                     /translation="MSPFAKKLPFPFTPMHGAYVLLLFYAVGVAGIVSPWSAQFRFLT
                     PYNLLLSAFVLLAFHRKYDGKFWGFLISCVALGFGSEWLGVHTAIIFGHYSYGSVLGF
                     KLDKIPIVIGLNWYLLAYITVDLISRIKIPIGVKVILGAALMTGMDYLIEPVAIRLGF
                     WYWHSGVIPMSNYVGWFIVSLPLQWIGHKYYSGNNPVAPWLFIAMLFFFLTLHFI"
     gene            complement(5145..6617)
                     /gene="crtI"
                     /locus_tag="PK281_04295"
     CDS             complement(5145..6617)
                     /gene="crtI"
                     /locus_tag="PK281_04295"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004570978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HOY48597.1"
                     /translation="MKQKRVIVVGSGIAGLAAAIRLACKGYAVQVFEKNSYPGGKLTA
                     FVQDGYRFDAGPSLFTLPKQVDELFELAGENPRAHFNYQRLPVACHYFWDDGTFLPAP
                     GNESDFAEEVEKVLYVPAERVLTYLNKSKFIYDTTAPVFLHRSLHIARNFLNLETLRG
                     IIRMPRLGIFSNQHHDNVKQLGDPKLVQLFDRYATYNGSDPYQAPGVLQSIPHLEFGQ
                     GAWFPDGGMHSITTSLVALAKRKGVEFVFEKAVDEILVSQNQICGVHCGNETHDADLV
                     ICNSDVVPAYRKLLASQQAPEKALSQPRSSSALIFYWGIKQVFSQLNMHNIFFSNNYE
                     EEFRFIFEGKQISSDPTIYINLTSNYLPSDAPDGCQNWFVMINVPGNTGQNWDQLIAQ
                     ARKDILAKLSRRLGTDIESLIQCESILDPRSIEQKTSSFQGSLYGSSSNNRYAAFLRH
                     ANRSSKIRNLYFCGGSVHPGGGIPLCLQSARIVSDLISPT"
     gene            6931..8661
                     /gene="recJ"
                     /locus_tag="PK281_04300"
     CDS             6931..8661
                     /gene="recJ"
                     /locus_tag="PK281_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013069750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HOY48598.1"
                     /translation="MSNAPVVKRWTLLPDGDSELVDSLTQQLGIERKLANMLVQRGVK
                     TFEEARSFFRPSLSELHDPYLMKDMNKAVDRILLALESDETIMIYGDYDVDGTTSVAL
                     LYSFFRELCPERLHFYIPDRYAEGYGVSFRGIDVAEELGCTLIIALDCGIRSVDKVDY
                     AANKGIDFIICDHHLPGSEVPKASAVLDPKQSDCAYPYKELCGCGIGFKLIQALKQVL
                     PHAGDTDHWLDLVATAIAADIVPITGENRILAYFGMLQINTKPRPGIRMLLRSGKDGA
                     NSNPTKEFSISDLVFTAAPRINAAGRIDHGKKAVELLITENESTAEEILSHINTQNVK
                     RKDLDQLMTSEALAILDSNELYRDVKSTVLFKNDWHKGVVGIVASRVMEKYYRPTIIL
                     TESNGKATGSARSVKGFDVHEAISACADLLEQFGGHKYAAGMTLPLENVEAFRQRFEE
                     IVAETIQEESLVPVVDIDMELELSDITPKFYRIMKQFAPFGPGNMSPVFLTKHLQDDG
                     YGKIVGTHHLKLKLGKSGQPSFDAIAFGMAHMHPAISKGIPVDVCYTLEENHWNGKVN
                     LQWMVKDMRC"
     gene            complement(8719..9945)
                     /locus_tag="PK281_04305"
     CDS             complement(8719..9945)
                     /locus_tag="PK281_04305"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014085366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HOY48599.1"
                     /translation="MNKIKVANPVVELDGDEMTRIIWHFIKDKLILPYLDLDIKYYDL
                     GMEHRDATNDQVTIDAAEAIKKYNVGIKCATITPDEQRVEEFGLKQMWKSPNGTIRNI
                     LDGTVFREPIVCKNVPRLVPNWTAPICIGRHAFGDQYRATDFVTKGKGKLTIKFEGED
                     GQVIEHEVYNFKGDGVALAMYNTDESIRGFARSCFNQAISKGWPLYLSTKNTILKKYD
                     GRFKDIFEEIYQAEFKATMDAAGITYEHRLIDDMVASALKWNGNFVWACKNYDGDVQS
                     DTVAQGFGSLGLMTSVLVTPDGKTMEAEAAHGTVTRHYRMHQQGKKTSTNPIASIFAW
                     TRGLEHRGRLDNNEALINFCQTLEKVCVDVVESGKMTKDLAVCIYGNKVSDDQFLYTE
                     DFLEALNQELKSRLGA"
     gene            10177..10968
                     /locus_tag="PK281_04310"
     CDS             10177..10968
                     /locus_tag="PK281_04310"
                     /inference="COORDINATES: protein motif:HMM:NF013166.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOY48600.1"
                     /translation="MHISFSKGEIRYKAEGQGPAIVFLHGHMESAKIWKKYASELSKR
                     FRTIRIDLPGHGKSSVFDEVHSMEFMADAVRAVFEAEKIDKALLIGHSMGGYVALAFA
                     ETHPDKVRGLVLFSSISFADTKERQNERDRAIKAAEAHKMKYITSVIPNLFFERSGAK
                     ASKRIFKLVKIAAKQPKEGITAAIRGMRDRADRTKILKNAKFPILFLAGHDDFLIPLE
                     RIEEMHRQVPNAKLVVIEQCGHAGFIEQEKECFTAIKEFAVEKIL"
     gene            complement(11017..12057)
                     /gene="thiL"
                     /locus_tag="PK281_04315"
     CDS             complement(11017..12057)
                     /gene="thiL"
                     /locus_tag="PK281_04315"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HOY48601.1"
                     /translation="MLQQPANKTGLETLGEFGLIDKLTNGFEIQNESSKKGIGDDAAV
                     MNYGDRDVLVSSDMLLEGIHFDLTYMPIKHLGYKAAVVNFSDIYAMNGKPEQLTIGLA
                     CSNRFPVEALEQLYEGIAMACKNYGVDLVGGDTTSSRAGLVLSLTVLGSVEKGKAIGR
                     DGAKKGDLLVVSGDLGGAYVGLQLLEREKSVFMDHPDAQPDLEGNDYILERQLKPEAR
                     RDVVELLSEMGVTPTSMIDVSDGLSSEIMHLCKNSSVGCALYEEKIPIDPTTYNMARE
                     FALDPTVCALSGGEDYELLFTIKQEDFEKVKGNMLVSIIGHMTDADEGIRMIDKSGGV
                     HELKAQGWNSFQ"
     gene            complement(12060..13061)
                     /gene="dusB"
                     /locus_tag="PK281_04320"
     CDS             complement(12060..13061)
                     /gene="dusB"
                     /locus_tag="PK281_04320"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009412898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HOY48602.1"
                     /translation="MARIGTIELGEFPLLLAPMEDVSDPPFRLVCKQYGADMMYTEFI
                     SSEGLIRDAVKSKKKLDIFEYERPIGIQLFGSDIDSMREAAEIAAAAEPDLIDINYGC
                     PVKNVACKGAGAALLQDIPKMVSMTSEIVKCTTLPVTVKTRLGWDDSTKNILEVAERL
                     QDIGIQALTIHGRTRAQLYKGPADWQLIGEVKNNPRIHIPIFGNGDVDSPEKAVEMRN
                     QYGVDGVMIGRASIGYPWIFNEIKHFMRTGEHLAAPTIADRVKVCRTHLDASIEWKGP
                     VLGIIEMRRHYTNYFKGIPHFKEYRMRLVTLPTYPEVQAVLDEIETVMGNLPTEINA"
     gene            13208..>13749
                     /locus_tag="PK281_04325"
     CDS             13208..>13749
                     /locus_tag="PK281_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48603.1"
                     /translation="MPRNRFQLYPPALQDAGPTRRFIWLLILCLIGISLASFISVAIG
                     SLIFGFSVPELAGLANNPNQPGALRVLQISQLFSSCGIFLFPPVMLVYLQEVPIRKGL
                     YLSGKPNMILFILAMLLMWLQLPIINQLAAFNNLLVFPDALKPIGDWMLAQEAQAAKL
                     TELFLTMSSPVDFISSLLIMA"
BASE COUNT         4153 a         2801 c         2795 g         4000 t
ORIGIN      
        1 cacagaatgc cgtaatgcca gtgaattgtg catcaggttg cgaatttgtt actgtaaaag
       61 gagcagaaac acctgtacaa ccatttgagt cgatcacagt aactgtgaaa gttccttgat
      121 tgacaaagtt tattggttga gtagagctgt tcgaccaagt ataggaatca taaacttcag
      181 tcaatccaat ctgaactaat ccatcaaaac aagaatgatt atcaccttca attactggtt
      241 gaggattagg attaactttt acaaattcag taacaggagg tgaaaaacaa ccgtcaattt
      301 cataagaaag ggaaatgcct ccatccatag aagggttaat gttattgtcg atatatgggt
      361 tgcctgtatg agtagatcca tcaggataag tccaagtata aatcgcatta ctgaaaacag
      421 aatcaggggt aaatgttaaa cttcctcccg tacatgaatc cccaagtgca ataaatccag
      481 atggatttgg cgatggagaa atttcaacag tggtagtacc tgtaaatgtt tctgttggat
      541 tggttgtgac taatgtataa acaccggcca tgtcttctgt aatgtttgga atcacagggt
      601 tagcttctgt actagtaaat ccattgggtc ctgtccaact aaaagcgtat tcaacctcat
      661 taaaaaaact caccaattct aagtcctgtc cagcacataa tggaccattg ttgaaaggtc
      721 cacaacttgt tgagcctgat ccactatttt gtgagaaaat gatgttacag ggttcttggg
      781 aaaaattcgt aatcaaaatc atataatatt ctcccgttat tccattgggg atattaacat
      841 cttcctcttc attcccagaa tagctgcaat ctacggtatt gtctgctgta agttgagaac
      901 agacatctac taatgaggta aacggaccat aacaaataaa gtcaatatca ttggtgggtg
      961 tattatcaat ggtgtcattt gtcccaacca tatgaatgtt tattgttcct gtattttcaa
     1021 tttgaagaaa ataccacgaa ggatttggtt cagaacctaa acaaccatag tcgggaccgg
     1081 cttcagctgc ttctccactt gttcctgcag gaaaattgaa ttgggttcct gtacaaaaag
     1141 gcgaagcatt actgcaactc gtgtttgaac cttgagcatt tgcagctaca gtagcgatga
     1201 acaacagtag aacacttgcg ataaaatttt tcatttgagt atcttgaaat ggttgaaaac
     1261 aattagtact tgtcggtttt cgcattaacc ggaagttgag caattgcttg ttgaatgcga
     1321 ttgatttctt gttcagcatc tgagattgta aaaactttag aacaagaaat cttcaattct
     1381 cctgattcgg agatacgaga gataactgca agtattttag aactatacaa ttgattaaca
     1441 aacgctgaac gttgcgcttc gttaagcgat tgcatgttta aagaaaagaa aattcgatgc
     1501 ttgttttctg aagttttctt aagaatgact tcagttggct taaccgtttc ttggttagtt
     1561 atagtttgtg ctacaagtga ttgactaatg cacacgcctg ttaaacagaa gaaatacaaa
     1621 gcaatttttt tcatgaagta aatttggagt gctaaggtag ttaatttgtg cagaagtatg
     1681 aaagttgtca ggtgtttata gaataaacga cctatttatt ttggttttga ttcatttgtc
     1741 tcatcgggaa aagttattgt ttttcctgtt aaacaagatt gtaatgcttt gataattaat
     1801 ttgtaatcat tgtcgagcca agcaaattgg attaggtgac ttcgactttt aaaagcatca
     1861 ttttgtgcat attcattaac aagcattcga tgaagaaatt taacatatct gttattttct
     1921 ccaattaact gatttccatt tttgtaatca gttagtttag ataaagtagt ttttacatgg
     1981 gttctcgcaa taagcgattg ctccatcatc atagccttat ttcgactcaa acttttagga
     2041 tgaacacgaa ttagtgaaag ggaatctgga gctggatcat aaacaagccg ataattcagc
     2101 gcaaaacaac ggaaataaaa atcccaatct tctacccaat tcaaaccttc aaaaaatccc
     2161 cccgtatctt ccagaacaca acgtttaagc aatggagaag atatcacaaa aatattccga
     2221 tcaaacattt tagctagcaa atcccaagtt cgacctgaat attcttccat ccaaggtttg
     2281 ttttgtgttg gatcgtgact aaaccgcaaa acatctggaa taccgtggtc gtaataccgt
     2341 gcattgctgt aaacaataga tgcatccgga tttttatcga acaaggctat ttgattttca
     2401 attttaccgg gagcgatttg atcatcagca tccacaaact gaataaagtc gccaatactt
     2461 ttagctactc ctgcatttct acagcctgaa gttgtcgtgt gagaaatgta taaatattta
     2521 aaacgagaat ccttttgttc ccaaagagct accgtttctt ttgtattgtc ggttgagcca
     2581 ttatcaacca ctatgcattc ccagttttga tatcgttgag attcaatact gactaatgtt
     2641 tctcctataa aatgagcata gttgtatgtc ggaattataa ccgacacttt cggttcaagt
     2701 gtcattcgtg aggaacgaga atcaatcgtt cggtatagct tttaacctaa actccatgct
     2761 gtaatcgaga taaatcgaat caggcattgg acttaataat ttgaattcag tgcggaactg
     2821 atacttctct ttttgaataa tacttgccca atcatcgtta ggaacaagca tatcaatagc
     2881 cataaaatgc tctgtggttg gaagctcagt aaaatgagca atctcaactt ctttgaacaa
     2941 atttttagga gaggataaat aaacagtcat tgaatcagca aatccaaaat gtgtcgctcc
     3001 tgagtcaatc aaaatggtaa gtgtaaacgc ctctacactt tctacgcttt gaggagtcga
     3061 tttattggtt tcgaacttat cgtaatcttt gaaagaatat tctgcactca taaagctagc
     3121 atattcattc ccaacaatcg tgtcggttaa aacagtgtcg taagcggttt cccagaatac
     3181 cttgtcgtga gtattcaaat tgaaactaac cagttgatcg agtttcttgc atgaaaatgc
     3241 agtaatagca atcaaaatag caagggcgta gggaataaaa cgcatggtag aaagacatta
     3301 gaatttcaaa aatagcggat ttttctgatt tcccatcaac gagtatcaat ctctcgttga
     3361 ttccgcttgg caaaactctc gtataatgcc acatttaaga gtaataataa catgcaataa
     3421 gcaagatctt caatggggat attcgaaatc ccttggataa aaatccgaac accggaattc
     3481 tctgcatcgt tatacatcac aactggtttg gatgtgagaa caccattcac aatcaaaaat
     3541 ggtataaggc agaagaaata acccacaaaa aaacgtccga ggtattttac tcgtttcaca
     3601 taggtgagat acaagagcaa taatgccgga agaaagcaag tataagctgt ataccaacga
     3661 tcgtagaaag caatagcaga tgtgagtaaa atcaaactaa ctgtcgtagc tattatgcgt
     3721 tgaaaaggac ctaaaacatc cttccggaaa aaataagctg tcacgaaata cacgaaaaag
     3781 caacaatacg gaacaatcaa aaaaaacaac cattcctcaa gcggtaagct gccaatgtta
     3841 attcctagcg tataatccgg actaaaactc cacacaccaa ttcgggtgaa aaattcatcc
     3901 caaataagga aaaagccggc tgtaagtaat atagctggaa acatgtgctt ccattttttc
     3961 cacaactcaa ttcttcgctc aaaactttgt gcaagtggat aggccaaact gcctatcata
     4021 agcgcgaggt agaggaattt atcgggcacg ttgttcttga aagaattttc gagctactat
     4081 caacattcca aacgattcgg aatgctcttt tcctaaggtc ttatgatgca ctttatgtgc
     4141 tttccgaata gcgcgcaaat accaattatc actgcgttta aaccagttta atcgttgatg
     4201 gataaatact tcgtgaacca agaaataagc aaatccataa actgcaatgc ctattccgat
     4261 atacaagcga aaatcattgc cattcatcat cccaaacata atgcacaacc aacttggaat
     4321 agcgaaaatc aaaaagaaag catcgtttcg ctcgaaaata tttgaatggg gttcatgatg
     4381 atcacgatgc aaaatccaca aaaatccatg catcacatat ttgtgcgtag cccaagccat
     4441 gaactccata aagaagaacg tagcgacaat tatacccgca ttaataagcc aattcatggt
     4501 tcagataaag tgtaaagtta aaaagaagaa cagcattgcg ataaataacc aaggagcaac
     4561 cggattgttt cctgaataat atttatgtcc aatccattgc aagggaagcg aaacaataaa
     4621 ccatcctaca taattgctca tcggaattac tcccgaatgc caataccaaa agcctaaacg
     4681 aatggcaact ggttcaatca aataatccat accagtcatg agcgctgctc ccagtattac
     4741 tttcacccca attggaattt taattcggga aattaaatca accgtaatat atgcgagcaa
     4801 ataccaattt agaccaatta cgataggtat tttatctaat ttaaatccaa ggactgaacc
     4861 gtaactgtaa tggccaaaga taatagcagt gtgcactcca agccattcgg agccaaagcc
     4921 aagtgctacg cagctaatta aaaagcccca gaatttccca tcgtattttc ggtgaaaggc
     4981 taataataca aatgccgaaa gcagcagatt gtagggagtt agaaaacgaa attgagcact
     5041 ccaaggtgag acaattccag ctacgcctac cgcataaaaa agcaggagca cataagcacc
     5101 atgcatgggt gtaaacggga atggtagctt tttagcaaat ggactcatgt gggagaaatg
     5161 aggtcggaaa caatgcgtgc cgattgcaaa cacagtggaa tgccaccacc tggatgtaca
     5221 cttccaccgc aaaaatagag attgcgaatc ttagaagaac gattggcatg tcgcaaaaat
     5281 gccgcgtaac gattattcga actgcttcca tacaaagaac cttgaaagga agaagttttt
     5341 tgctcaatac ttcgcggatc taatatgctc tcacattgaa taagcgattc aatatcagtt
     5401 cctaatcgac gactaagttt agcaagaata tcctttcgtg cttgtgcaat gagttgatcc
     5461 caattttgtc cggtatttcc gggaacatta atcatgacaa accagttctg acaaccatcg
     5521 ggcgcatcgc ttggtaaata attactggtg agattgatgt aaatagtcgg gtcggaactg
     5581 atttgtttgc cttcgaaaat gaaccggaat tcttcctcgt aattattcga gaagaaaatg
     5641 ttgtgcatgt tgagttgcga aaatacctgc ttaattcccc aatagaaaat aagtgccgaa
     5701 cttgatctag gttgcgaaag cgctttttcg ggtgcttgtt gacttgccaa taactttcgg
     5761 taagcaggaa ccacatcgct gttgcagatt actaagtcgg catcatgtgt ttcattgcca
     5821 cagtgtactc cgcaaatttg attttgggaa acgagaattt catccaccgc tttttcgaac
     5881 acgaattcaa cacctttacg tttcgctagt gcaactaaag atgtggttat actatgcatt
     5941 ccaccatcgg gaaaccaagc accttgtcca aattcgagat gaggaatact ttgcaacacg
     6001 ccgggcgctt gatacggatc ggaaccatta taggtagcat atcgatcgaa caattgaacc
     6061 agtttgggat cgcctaattg tttgacatta tcgtgatgct gattagaaaa aatgcctaaa
     6121 cgaggcatgc gaattatgcc tctcaatgtc tctaggttga ggaaattacg agcaatatga
     6181 agcgaacgat gcagaaaaac gggagcagtt gtatcgtaga tgaacttgct tttattcagg
     6241 taagtcaaaa cgcgttcggc tggaacatat agcacttttt cgacttcttc tgcaaaatct
     6301 gattcattac ctggagcggg aagaaaggtg ccatcgtccc agaaatagtg gcaagcaacc
     6361 ggcaaacgtt ggtagttaaa atgcgctcga ggattttcac cagctagttc gaaaagctca
     6421 tcgacctgtt ttggtaaggt gaataaagaa ggtccagcat cgaagcgata tccatcttgc
     6481 acaaacgctg tgagtttccc accgggatag ctattctttt cgaatacctg aacagcatag
     6541 cctttacatg ccaatcgaat ggctgctgct aatccagcaa tgcccgaacc gactacaatg
     6601 acccgttttt gtttcacagc acaaaagaac tcaattgtga gaagaatgtt cgctaaactc
     6661 tggaatgaat tctaggagtt gcagatggga atatccaaag acattactga atatatgttt
     6721 ttacacgctc gtggcaagga tagagcggat agcccggagt ctaagtcgaa gcatttgcga
     6781 aggcttacat cgaggactaa gagcgatagc ctggtcgcca cccaaatatt tttgaacaga
     6841 ggcgtggata agttttacgg ttagatgcat gatttactcc gattgggaat tggagggcag
     6901 taattttgac gattcatttc tgattgattt atgtcgaacg ctcctgttgt taaacgatgg
     6961 actttattac ctgatggcga ttccgaactc gttgattcct tgactcaaca acttgggatt
     7021 gagcggaagt tggcgaatat gttggttcag cgtggagtga agacgtttga ggaagcgcga
     7081 agttttttcc gtccttcgtt gagtgagtta catgatcctt atttgatgaa ggacatgaat
     7141 aaagcggttg atcggatttt gctggctttg gaatcggatg aaaccataat gatttatgga
     7201 gattacgatg tggatggaac gacttcggtg gcgcttttgt actcgttttt cagggaatta
     7261 tgtccggaga gattgcattt ctatattcca gatcgatatg ctgaaggata tggggtttcg
     7321 tttcgaggaa ttgatgtggc ggaagaacta ggctgcacct taattatcgc gctcgattgc
     7381 gggattcgtt cggttgataa ggtagattat gctgcaaata aaggcatcga ttttattatc
     7441 tgcgaccacc atttaccggg ttcggaagtg cccaaagctt cagcagtgct agatcctaag
     7501 caaagtgatt gtgcttatcc gtacaaagaa ttatgtggat gtggaattgg gtttaagctg
     7561 attcaggctt taaaacaggt tttacctcat gccggcgata ccgatcattg gctcgattta
     7621 gtagcgactg ccattgctgc cgacattgtc ccgataacag gtgaaaaccg cattctggct
     7681 tattttggaa tgcttcaaat taatacgaaa cctcgtccgg gaatacgcat gttattgcgt
     7741 agtggaaaag atggggcgaa ttcgaatcca acaaaagagt ttagcatttc ggatttagtc
     7801 tttacggctg ctccacgcat taatgcagct ggtcggatag atcatggaaa gaaagcggtt
     7861 gaattgctca tcaccgaaaa tgaatcaaca gcagaggaaa ttctatcgca catcaacacg
     7921 caaaatgtca agcgtaaaga tttggatcaa ctcatgactt cggaagcact ggccatactc
     7981 gattcgaacg aattatatcg ggatgtgaag agtaccgtgc ttttcaaaaa cgattggcac
     8041 aaaggtgttg ttggaattgt ggcttcacgt gtgatggaaa aatattatcg tccaaccatt
     8101 attctcaccg aatctaatgg gaaagccacc ggtagcgctc gttcggttaa aggatttgat
     8161 gtgcatgaag ctattagtgc ttgtgccgat ttactggaac agtttggcgg acataaatat
     8221 gctgccggaa tgactcttcc gcttgagaat gtagaagcat ttcgtcagcg attcgaagaa
     8281 attgttgccg aaaccattca agaagaatcg ttagttccag ttgtagatat tgatatggaa
     8341 ctagaattat cggatatcac gcctaagttt taccgaatca tgaagcaatt tgctccattt
     8401 ggtccgggaa atatgtcgcc cgttttcttg accaaacatt tgcaggatga tggatatggt
     8461 aaaattgtgg gaacacatca tttgaaattg aaattaggta aatcagggca accatcattt
     8521 gatgcaattg ctttcggaat ggcacacatg catccagcta tttcaaaggg aattccagta
     8581 gatgtttgct atacgttgga agagaaccac tggaatggca aagtgaattt gcagtggatg
     8641 gtgaaagata tgaggtgcta aaaacgaaaa agccccaacc gaagttgagg ctttaatttt
     8701 tgctgaatcg ggattaaatt aagctcccaa acgcgatttt aattcttggt tcaaggcttc
     8761 taagaagtct tcggtataca agaattgatc atcgcttact ttgtttccgt agatacaaac
     8821 tgccaagtct ttggtcattt tgccgctttc aacaacatcc acacaaactt tctctaatgt
     8881 ttggcagaag tttataagcg cttcgttgtt atctaagcgt ccacgatgct ccaaaccacg
     8941 agtccaagca aagatgctgg caataggatt ggtgctggtt tttttgcctt gttgatgcat
     9001 gcggtagtga cgagtaacgg ttccgtgagc cgcttcagct tccatggttt ttccatctgg
     9061 agtaaccaat acagaagtca tcaaccctaa tgaaccaaaa ccttgagcta ccgtgtcgct
     9121 ttgtacgtca ccatcgtagt ttttacaagc ccaaacgaag tttccgttcc atttcaatgc
     9181 tgatgcaacc atgtcatcaa ttagacgatg ctcataggta atacctgcag catccatggt
     9241 agctttgaat tcagcttgat aaatttcttc gaagatgtct ttgaaacgtc cgtcgtattt
     9301 tttcaagatg gtgttttttg tactcaagta caatggccat cctttgctaa ttgcctgatt
     9361 aaagcaagaa cgagcaaatc cacgaatcga ttcatcggta ttgtacattg ctaatgcaac
     9421 gccgtcacct ttgaaattat atacttcgtg ctcaattact tgtccgtctt caccttcaaa
     9481 tttgatcgtt aacttacctt ttccttttgt tacgaaatcg gtagcacgat attgatcacc
     9541 aaatgcgtga cgaccaatgc agattggagc agtccagttt ggaactaagc gagggacgtt
     9601 tttacatacg attggctcac ggaaaacagt accatcaagg atgttacgaa tagtaccatt
     9661 ggggcttttc cacatttgtt tcaagccaaa ttcttccaca cgctgctcat ctggagtgat
     9721 ggttgcgcac ttgataccta cattgtattt cttaatcgcc tctgcagcat caatagtaac
     9781 ctgatcgtta gttgcatcgc ggtgttccat tcctagatcg tagtatttaa tatctagatc
     9841 gaggtaaggg agaatgagtt tatccttgat aaaatgccag ataatacggg tcatttcatc
     9901 tccgtcgagt tcaacgactg gattagcaac tttgattttg ttcatggtgt gatgatgaag
     9961 cggttaagcg aaaatgagtt attcatttcc gggctgcaaa ggacgttaac tgtgggcatt
    10021 tccgcaagtg agatttagaa aatattctgg agttttttta tcctcgaact gaagtccaag
    10081 gttacaattg aagaactgaa ttagctaaaa tgttaacatt ggaaatgcta taatcacttg
    10141 ggaattgatg tattttgcgc cccgaaaccc agctctatgc acatttcatt ttcaaaagga
    10201 gagattcgtt acaaagccga aggtcaaggt ccggccattg tttttctaca cggccatatg
    10261 gaaagcgcaa agatttggaa gaaatatgca agtgaattaa gtaagcgttt tcgcaccatt
    10321 cgaatcgatc ttcctggaca cggaaaatca tccgtttttg atgaagttca ttccatggaa
    10381 ttcatggctg atgcggttcg agcagtgttt gaagcagaga aaattgataa agccttgcta
    10441 attggtcact caatgggcgg ctatgttgca ctcgcatttg cagaaacaca tcccgataaa
    10501 gttcgtggat tggtgctttt tagctcgatc tcttttgctg atactaaaga gcgtcaaaac
    10561 gaacgcgacc gggcgattaa agcagccgaa gcgcataaaa tgaagtacat tacttctgtt
    10621 atacccaatc tgtttttcga acgaagtggt gccaaggcta gcaaacgaat attcaagtta
    10681 gttaagattg cagcaaaaca acctaaagaa ggtataaccg ctgctattcg tggtatgcga
    10741 gaccgtgctg atagaactaa aattctcaaa aacgctaagt ttccgatact ctttctcgct
    10801 ggacatgacg atttcctaat accacttgag cgcattgaag aaatgcaccg tcaagttcca
    10861 aatgccaaac tagtcgttat agagcagtgt ggccatgccg gatttattga gcaggaaaaa
    10921 gaatgcttta ctgccataaa ggaatttgca gtagaaaaga ttctctaatg tgaaaactaa
    10981 ccagcacatc agcacactag cgcatcagca catcgtttac tgaaaactat tccaaccttg
    11041 agctttgagt tcgtgaactc ctccgctttt gtcgatcatg cgaatacctt catcggcatc
    11101 ggtcatgtgg ccaatgatgc taacgagcat attgcctttc actttctcga aatcttcttg
    11161 cttgattgta aagagtaatt cgtaatcctc acctccgcta agtgcacaaa cagttgggtc
    11221 gagtgcaaac tcacgagcca tgttataagt agttgggtcg atgggaattt tttcctcgta
    11281 aagtgcacaa ccaacactac tgttcttgca caaatgcatg atttcagaac tcaagccatc
    11341 cgaaacatca atcatcgatg taggcgtaac gcccatttct gaaagtaatt ctaccacatc
    11401 acgacgcgct tcgggtttca gttgacgttc gaggatgtaa tcgtttcctt ccaaatctgg
    11461 ctgtgcatcg ggatgatcca taaacaccga tttctcgcgt tcgagtaatt gcaatccaac
    11521 atatgcacca cctaaatcac ccgaaacaac tagtaaatca ccttttttag ctccatcgcg
    11581 accaatagct tttccttttt caacgctacc taaaacagtt aaacttagca ccaaacctgc
    11641 acgggaggaa gtggtgtcac cacctactaa atccactccg tagtttttgc aagccatggc
    11701 aattccttcg tacagctgct ccaaggcttc aactggaaaa cgattggaac acgctaatcc
    11761 aatagttaac tgttccggct ttccgttcat agcatagatg tcggaaaagt ttactaccgc
    11821 tgctttgtag cctaaatgct tgattggcat gtatgtcaag tcgaaatgaa tgccttcgag
    11881 caacatatcg gatgatacca atacatcccg atcgccataa ttcatcacag cagcatcgtc
    11941 gccaatgcct tttttgctgc tctcgttttg aatttcgaag ccgtttgtga gtttgtcaat
    12001 gagaccaaat tcacctaaag tttcgagtcc ggttttattt gcaggttgtt gaagcataat
    12061 caggcgttta tttcggtagg gagatttccc attaccgttt caatttcatc gagcaccgcc
    12121 tgaacttcag ggtaggtggg caatgtaaca agtcgcattc ggtattcctt aaaatgtgga
    12181 atgcctttaa agtaattcgt gtaatgacga cgcatctcaa taatccctag aacaggacct
    12241 ttccactcaa tggaagcatc gaggtgagtg cgacacactt ttacacgatc ggcaattgta
    12301 ggtgcagcta aatgttcacc cgtgcgcatg aaatgcttga tctcattgaa gatccacgga
    12361 tagccaatgc tggcgcgacc aatcatcact ccgtctacac catattgatt ccgcatctca
    12421 acggctttct caggagaatc cacatcgcca tttccaaaaa taggaatgtg aatacgtgga
    12481 ttgtttttta cttctccaat gagttgccaa tcagccggtc ctttatacaa ttgcgctcgt
    12541 gttctaccat gaatagtaag cgcttgaatg ccgatgtctt gaagtcgttc ggctacttct
    12601 aagatgttct tggtcgaatc gtcccaacct aaacgggttt tcacggtaac cggaagtgtg
    12661 gtgcatttga caatctcact cgtcatcgac accattttag gaatgtcttg aagtaaagca
    12721 gcaccggcac ctttgcaagc tacattcttc accggacaac cataattgat atcgatcaaa
    12781 tcgggttcgg cagctgcagc aatctcagct gcttcacgca ttgaatcgat atcgcttcca
    12841 aagagttgaa ttccaattgg gcgttcatat tcgaaaatat cgagtttctt tttgcttttt
    12901 accgcatcgc gaatgagtcc ttccgaagaa ataaattcgg tgtacatcat atcggcaccg
    12961 tattgcttgc aaaccaaacg aaatggtgga tcgctcacat cttccattgg cgctaacaac
    13021 aatggaaatt cacccagctc aattgtacct attctagcca tgttctttca gccggcaaaa
    13081 ataggcataa atcccgctct ttcagctagt ctgattcaat cgtcattttg aatctcacat
    13141 ttttccaaaa tcctcataca acacgtacaa ttttatactt tcacccaatc gttacaaccc
    13201 aactcgaatg ccgcgtaacc gctttcaatt atatcctccc gctttacaag atgctggtcc
    13261 aactcggcga tttatctggc ttttgatttt gtgcctgatt ggaatctcac ttgcatcgtt
    13321 catttctgtt gctattggca gtttgatatt tggtttttct gttcccgaat tagcaggttt
    13381 agcaaacaat cccaatcaac cgggagcttt acgtgtgttg caaattagtc aactgttttc
    13441 ttcttgcgga attttcctgt ttcctcccgt tatgcttgtg tatttgcaag aagtccccat
    13501 tcgaaaagga ctttatttat cgggcaaacc caacatgatt ctattcatat tggcaatgct
    13561 gctaatgtgg ctgcaactgc ctattatcaa ccaattggca gcattcaata atttactcgt
    13621 ttttcctgat gcacttaaac caataggaga ttggatgttg gctcaagaag cgcaagcagc
    13681 taaattgacc gaattgtttt taaccatgtc gagcccggtc gattttatta gttccttgct
    13741 catcatggc
//
LOCUS       DAOOQW010000044        13547 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_5581_length_13547_cov_4.598873, whole genome shotgun sequence.
ACCESSION   DAOOQW010000044 DAOOQW010000000
VERSION     DAOOQW010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 13547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13547
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5581_length_13547_cov_4.598873"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(435..1637)
                     /locus_tag="PK281_04330"
     CDS             complement(435..1637)
                     /locus_tag="PK281_04330"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOY48604.1"
                     /translation="MLYKVLHLLIGAWMRIHLKRLFIRGLDNVPKNEPIILTCNHGNA
                     FLDAVLVAITLKRPVHFLTRADVFKKPWQRYILGKIKMIPVYRIRDGLDSLEQNNETF
                     SRCSKILKDGGAILIFSEGNAHPEKRLRPLKKGSARIAFQSAEAMGWTSDLKIIPVGI
                     NYIEHTRFRTEVMLGFGPSITVSDFRSEYESDQARGLRAINHQIFQGIQQEMIHIPER
                     AQDDFANIALAIGRAEKQYPLFRFRYLDEGRLDHEQQVLANFIERDSPDLRDKLHRFA
                     KKSGELHLPLSLAHAQVPQGRTAFLILCFIPAFLGLIIHALPMSLAFWFTGKKVRDAQ
                     FINSVLMGTGFFFTAFWYLFIIISKAIYAPYLLFFIPFFPILLRITLLYSEALSQQRA
                     RALRKKMK"
     gene            complement(1884..2636)
                     /locus_tag="PK281_04335"
     CDS             complement(1884..2636)
                     /locus_tag="PK281_04335"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOY48605.1"
                     /translation="MKRIYLVEDEPLAFKRLSKLILELEPNWEIVGHADSVESAVDFL
                     KKMPSPDLIFMDIQLADGISFSIFESVEIKAPVIFTTAYDEYALKAFKVNSIDYLLKP
                     IDIDELRNAISEFKQRSTSNQPDITSILQEINANKNYRSRFLLTKGDALIPVETSDIA
                     WIVAEDKLVFLIQNSGNKSLFSFTLDELTTQLDPKDFFRINRSVIAHRKSIQKAELHF
                     NGKIKIHLNPATETEQFVSREKAASFKDWWGS"
     gene            complement(2641..3714)
                     /locus_tag="PK281_04340"
     CDS             complement(2641..3714)
                     /locus_tag="PK281_04340"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOY48606.1"
                     /translation="MSQENPHSLFSGTRIANVKDILLMVFVGVLIVSYMFVKNLIQGN
                     VAEQAPDTVVNNALFSISISIILYVVFRVMFRYLNTHYPWLKSIGKRLQIQLLLVVGL
                     SSTIMALFTWLWYLLFFDGCFSRSSFFSNIIIAVLISLLINAIYEGVNLFRQLKDSQI
                     ETEKLKRQNIESHFETLKNQVGPHFLFNSLNTLLALIDEDTLQAKEFVKKLATYYRYA
                     LQVNERDVVELTTETALVKSYVFLLESRFGDNLKVYFDLPEQFDNKGIVPLSMQMLVE
                     NAVKHNVISNAYPLNIQIGIKDGFIFVENSKRKKDALEQSNRIGLENIRNRYQLLAGK
                     DIRVESEHPDYFRVELPLIELVK"
     gene            complement(3718..4389)
                     /locus_tag="PK281_04345"
     CDS             complement(3718..4389)
                     /locus_tag="PK281_04345"
                     /inference="COORDINATES: protein motif:HMM:NF021576.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2306 domain-containing protein"
                     /protein_id="HOY48607.1"
                     /translation="MKTLHTFLLILHVAGGMIGLITGLLNISNKKGARFHKLFGKIYV
                     GGMFTATISGTVLAAFGGNHFLFLIGIFSFYLAFSGLRSIQHKMQNGEVHYAIVDKLV
                     AGLTGIFGASMIALAAIQPVGSSKHIAPILLVFGSISLIFSLGDLLLFVGVRKIKDLK
                     NRWFFNHIGRMIGSYISAVTAFLVVNLDNLLPPLVIWLGPTVIGTIFITGFTTYYKRK
                     FAIGK"
     gene            complement(4403..6826)
                     /locus_tag="PK281_04350"
     CDS             complement(4403..6826)
                     /locus_tag="PK281_04350"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOY48608.1"
                     /translation="MKTNIFEGRFIRINKNILLVQFTIILASLFSLSTKVTAQELTQT
                     IRGTVSDAASQSPIPGATVVLVGSNPLKGVSADLDGNFRLIDVPVGRPVIQISMMGYQ
                     PVKLSNLVLTSTKELILNINLESSAIQGKEVEIVAKIDKDKPLNELAGVSARTFSVDE
                     AQRYAGSFGDPARLATSFAGVVAGNDQRNDIVVRGNTPLGVLWRLEGIDIPSPSHYSG
                     AGTSGGAVSILNTNVLANSDFLSGAYAAEYGNATSAAFDVKMRKGNNEKHEFTGQIGF
                     NGFEIGAEGPLSKNKKSSYLINYRYSTLGAFNAVGIEFVKGGVPQYQDISMKLHFQHK
                     NGYTSLFGIAGTSDIHFESEKDSLVWVKDPAKRQDLKNGSDMAVVGLTHVHFLNTKTS
                     LRTTVAATGNRFRTSIDSISPSYAMLDNFRSKMEESKLTGTAVLNSRLNSKTTFRAGV
                     IVSHLMYNSDVNRYSASLNARQQLIDFNGNGDLVQGYAQWHYKAGERWTLDAGIHALH
                     YYVNNQSSLEPRAAISFQATGNQRISIGYGNHSRVLPITIYLRETQLADGNMIQTNKN
                     LDLLRSNHFVAGYDWLIREHLRLKAEVYYQQQYNVGVQDGKLNSLSVLNYGANFGDVI
                     GPDSLTAKGKGFNKGVELTLEKFFHKNYYFLMTASLYDSKYKGSDGIERNTAFNNKYA
                     FNLLAGKEIPVGKTKQNVLVFSFRQVVTGGMWMTPINLEASQQMGFEIRNEEKAYSEQ
                     LPGYLRTDIRIGFRRNKKKITEEYGLDFQNLTNHKNLFSRSYNSSSKKIENNYQVGLF
                     PMMMYRITF"
     gene            complement(7120..7965)
                     /locus_tag="PK281_04355"
     CDS             complement(7120..7965)
                     /locus_tag="PK281_04355"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOY48609.1"
                     /translation="MALSNHQTRVITGAIFGIVLIGGILLSEYALTSIALFILLVGCF
                     EFNRLVHALSINTNRAFLYLANILVFAACIYFSMTQHSIGLVEHDTFLFLSIFIGLLF
                     LLFAWELFRGIPKPLENLASSILSLIYLGVPCGLLVSCSIGNDGNYLPWNVLYLFFFM
                     WASDTGAYFTGRAFGKHKLFERLSPKKTIEGFIGGIVTAMLVGIAAYYFLGGASLPMW
                     MLIGGVLSVTGSMGDLFESMLKRQANIKDSGTILPGHGGILDRFDSTLLSAPIYWVLL
                     HFIQS"
     gene            complement(7975..8205)
                     /locus_tag="PK281_04360"
     CDS             complement(7975..8205)
                     /locus_tag="PK281_04360"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HOY48610.1"
                     /translation="MENQWTVIASDTNVSRLYMLLNLMEQKDIPAQILNKVDSVYPSI
                     GEAELYVDAAFRLKALQIIHEAQNESPDTFQD"
     gene            complement(8284..10446)
                     /gene="ftsH"
                     /locus_tag="PK281_04365"
     CDS             complement(8284..10446)
                     /gene="ftsH"
                     /locus_tag="PK281_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007646506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOY48611.1"
                     /translation="MAEQPQNNTPGTPKNSGNRRLFPQSGKPKPSSGGGSNFYWIYGI
                     ITLLFIGSYFYNVGTNTREIKTKEFTEMLGAADVKEITVVNKEYAEITIKKEKLSKPE
                     YKNLKSPNMSASLEKGPHYNYTIGSVEDLNKIIDTAQVSVAPELRIHPSYESRKNWSG
                     EIISWVILLGVVFLFWMLLIRRMGGGGGGGQIFNIGKSRAQLFDKDTHVTVTFDNVAG
                     LDEAKVEIREIVDFLKNPKKYTILGGKIPRGALLVGPPGTGKTLLAKAVAGEAQVPFF
                     SLSGSDFVEMFVGVGASRVRDLFKQAKEKAPAIIFIDEIDAIGRARGKNPMAGANDER
                     ENTLNQLLTEMDGFGTNNGVIILAATNRADILDRALLRPGRFDRQIYVELPDLNGRRE
                     IFKVHLKPIKTDQSLDIEFLARQTPGFSGADIANVCNEAALIAARKNKDVVGKQDFLD
                     AVDRIIGGLEKKNKIISMDEKRVIAFHEAGHASVSWLLEHASPLVKVTIVPRGQSLGA
                     AWYLPEERSITTFEQLLDEMCAALGGRAAEEVTFGKISTGALSDLEKITKQAYAMVTI
                     YGLNDKIGNISFYDSSGQSEYSFNRPYSEKTAETIDQEVKKLIDMAYERTKKILQENK
                     TKLDSLATILLEREVIFREDLEEIFGKRIWDKDDESASSDISRSYKDQLKDNQDIAAA
                     NAAKEKSEEKIEPSNLSKPDTSTVSKEEEARTNPSEEA"
     gene            complement(10507..10896)
                     /gene="rsfS"
                     /locus_tag="PK281_04370"
     CDS             complement(10507..10896)
                     /gene="rsfS"
                     /locus_tag="PK281_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013551091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HOY48612.1"
                     /translation="MKKKAASIATDSAEALIDPIIKGIEEKKGKNIQVFDLRQTGHSV
                     CDFFVICNADSGTQVDAIAYSVEHEVKVATGESPWRSEGFENQEWILIDYVNVVVHVF
                     QTSIRDFYKIERLWADAIVRVIPESVA"
     gene            10938..11690
                     /locus_tag="PK281_04375"
     CDS             10938..11690
                     /locus_tag="PK281_04375"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOY48613.1"
                     /translation="MMELLFMGRNLIRLDSCASTNNFAASLLEQNAPEGTLVVAKEQT
                     GGRGQRGKTWYAEPGKNLTLTYILRPLSLRIADQFLLNKTIAIAVARTVELISPGSFI
                     RIKWPNDIFLENRKLAGILVETAVKGSQIVSCLAGIGINVNQTVFPDDSGNPVSLAME
                     LETQLVLDDVMMLLSKQMEVYYFRFSSSHKALLEEEYDSLLWKRGEVYPFKFNSEDFP
                     AEIVNVDEQGKLVLKTTEGEERRFSHGEIDWF"
     gene            complement(11695..12432)
                     /locus_tag="PK281_04380"
     CDS             complement(11695..12432)
                     /locus_tag="PK281_04380"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HOY48614.1"
                     /translation="MKFICTVIASILFINLHSQELPAIKVTGVANMEVTPDIAVFKLH
                     LNARNKSESLSIQDLTVVSNNVLTKLKELGYPEKEITLNDYSVVANYEYVGDKAKRNG
                     YRATQNLTVKTRLSKEIILKTLKAFSTEDARQTYITYNSVCSDSLQKAVEKKLIEHAI
                     TDASEKATIIATSLKVKLAQVQQVFYEVNEGYGSGNGAGGVDYYATSSGKSMNEMELV
                     NETYFTIEKETFIKSVRIDFGIEQNKP"
     gene            complement(12471..13505)
                     /gene="hutI"
                     /locus_tag="PK281_04385"
                     /pseudo
     CDS             complement(12471..13505)
                     /gene="hutI"
                     /locus_tag="PK281_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805282.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
BASE COUNT         4194 a         2710 c         2424 g         4219 t
ORIGIN      
        1 atctcaaaaa acgaggctcc ttcaaaccac ttcccgattg gatgttacgc accctaggct
       61 ggggacgtgt gaaagattga tttaatgtgc taatatgctg atggaagatt cagcatatga
      121 ctgcgctgta actttataac gaaatataaa cagcacatca acacactagc acaccagcac
      181 atcaattcaa tgtgctgatt agttgatgtg ctaatatgct gatggaagat taagcaaatg
      241 gcttgctcta actttataac gtaataaaaa cagcacatca acacactagc acaccagcac
      301 atcaattcaa tgtgctgatt agttgatgtg ctaatatgct gattgaagat tcagcatatg
      361 actgcgctgt aactttataa cgaaatccaa acagcacacc aacacactag cacatcagca
      421 cattaattcg cacatcattt cattttcttt ctcaaagccc gagctcgttg ttgggacaat
      481 gcttcagaat atagcaaggt aattctcagt aaaatgggga aaaaaggaat gaaaaagagt
      541 aggtaaggag cataaatagc tttgctgatg atgatgaata ggtaccaaaa agcggtaaag
      601 aaaaagcctg tgcccattaa taccgaattg atgaactgag catcacgaac cttttttcct
      661 gtgaaccaaa atgcaaggct catagggaga gcatgaatga ttaatcctaa aaatgctggg
      721 ataaaacaga gaattaagaa ggctgttcgt ccttgtggaa cttgggcatg cgcgagtgaa
      781 agtggcagat gtaattcccc actcttttta gcgaaacgat gcaatttatc tcttagatcg
      841 ggtgaatcac gttcgatgaa attagccaat acttgttgct cgtgatccaa tcgaccttcg
      901 tctaaataac gaaatcggaa caagggatac tgtttttctg cccgaccaat ggctagtgca
      961 atattggcaa aatcgtcctg agcacgttca ggaatatgaa tcatttcctg ttgaattcct
     1021 tggaaaatct gatggttgat ggctcttaat ccacgtgctt gatccgattc atattcactt
     1081 ctaaaatccg aaacagtaat agaaggtcca aatcccaaca tgacttctgt acgaaaacga
     1141 gtatgttcga tgtaatttat tccaactgga atgatcttca aatcgctcgt ccatcccatt
     1201 gcctcagccg actgaaatgc aattcgagca cttccttttt ttaaggggcg aagtcttttt
     1261 tcagggtgag cattaccttc agagaaaatt aaaattgcgc caccatcttt taggatttta
     1321 gaacaacgtg aaaaggtctc gttattttgt tccaagctat ccaatccatc ccgaatacga
     1381 tataccggaa tcattttgat tttacctaag atgtagcgtt gccaaggttt tttaaaaacg
     1441 tcggcgcgag taagaaaatg gacaggacgt tttaaagtga ttgcaacaag taccgcatct
     1501 aagaatgcat ttccgtgatt acaggtcagt atgatgggtt catttttcgg aacattatcc
     1561 aatcctcgaa tgaacaatcg tttgagatgg atccgcatcc acgccccaat gagcagatgt
     1621 aataccttat acaacatgcc cgcaagatag tattttagtg tgctggtgtg ctaatgtgtt
     1681 ggtgtgctaa tggtattatg cgagtgagtt attgtggaaa tttgcttgtg tgttactttg
     1741 ttgatgtttg attattgatg atagagggct tggcgtaaat acacgacaaa taacaaaacg
     1801 tatctaacag tcataatatt cagaaacaat acactagaaa ttaacagcac ataagcatat
     1861 taacacacca gcacatcatt ttcttaactt ccccaccaat ccttaaacga tgctgctttt
     1921 tctcgactta caaattgttc cgtttctgta gctggattta aatggatttt gatttttcca
     1981 ttaaagtgta attctgcttt ttgaatggat tttcggtgag caattactga acgatttatt
     2041 cgaaagaaat cttttggatc taattgagta gttaattcat ccagtgtaaa agaaaaaagc
     2101 gatttatttc cggaattttg aattagaaaa accaatttat cttcggctac aatccaagca
     2161 atatcagatg tttcaactgg aattaatgca tctccttttg taagtaaaaa tcgagatcga
     2221 taattcttat tggcgttaat ctcttgaaga attgaagtta tatcaggttg gttggaagtg
     2281 cttcgttgct tgaattcgga aatcgcattt cttaattcat caatatctat tggttttaat
     2341 aaataatcga tactattaac tttaaaggct ttcaaggcat attcatcata tgcagtggta
     2401 aagatgaccg gtgcttttat ttcaactgat tcgaaaatac taaagctaat tccatcggct
     2461 aattgaatat ccataaatat taaatcggga ctgggcattt tctttaaaaa atccacagca
     2521 ctttctacgc tatcagcatg tccgacaatt tcccaattcg gttctaattc aagaattaat
     2581 ttactcaaac gtttaaatgc caaaggctca tcttcgacta ggtaaatgcg tttcatgagg
     2641 ctattttact aattcaatta atggtagttc aacacgaaaa taatcgggat gttcactttc
     2701 gacccgaata tcttttccgg caagtaattg atagcgatta cgaatgtttt ccaatccaat
     2761 tcgattactt tgttctaatg catctttctt ccgtttacta ttttcaacga aaataaatcc
     2821 atctttaatt ccaatttgga tgtttaatgg atatgcattt gaaataacat tatgcttaac
     2881 cgcattttca accagcattt gcatggataa tggaacaatt cctttattat caaactgctc
     2941 tggtaaatcg aaataaactt ttaaattgtc accaaagcgt gattctaata ggaacacata
     3001 acttttgact aaagctgttt cggttgttaa ttctaccaca tctcgttcat tcacttgtaa
     3061 cgcgtatcgg taataggtag ctaacttttt aacgaattct tttgcttgta aagtatcttc
     3121 atcaattaaa gcaagtaaag tattcaaact attaaataag aaatgcggcc caacctgatt
     3181 ctttaatgtt tcgaaatggg attcaatatt ttgtcgctta agtttctccg tttctatctg
     3241 actatcttta agttgacgaa ataagtttac tccttcataa atagcattaa tcaaaagaga
     3301 aattagaaca gctataatga tattactaaa gaaagaactt cgagaaaagc aaccatcaaa
     3361 aaacaataaa taccatagcc aagtaaacaa agccatgatg gtagaagata atcctactac
     3421 caataataac tgaatctgta gacgttttcc tattgatttt aaccaaggat aatgtgtgtt
     3481 tagatatcga aacatcaccc taaaaacaac atacagaata atacttatac taatcgaaaa
     3541 aagcgcatta tttactactg tatctggtgc ttgttctgct acattacctt gaattaaatt
     3601 cttaacaaac atatagctca caatcagcac gcccacaaag accatcaata ggatatcttt
     3661 tacattggca atgcgggttc ccgagaaaag tgaatgcgga ttttcttgac tcatacctta
     3721 ttttcctatt gcgaatttac gtttataata ggttgtaaat ccggtaatga aaatagttcc
     3781 aataacagtt ggacctaacc aaattaccag tggtggaagt agattgtcta gattcaccac
     3841 taaaaatgct gtaacagccg aaatatacga tccaatcatt cgcccgatgt gattgaagaa
     3901 ccagcgattt tttaaatctt tgatcttcct gacacctaca aataataata aatcgcctag
     3961 cgagaatatc aaactgatac ttccaaagac tagtaatata ggggcaatat gtttcgaact
     4021 tcctactggt tgaattgcag ctaatgcaat cattgaggct ccgaatattc ccgttaatcc
     4081 agcaactagc ttatctacta tagcatagtg gacttctcca ttttgcatct tatgttgaat
     4141 ggatctcaat cctgaaaagg ctaagtagaa actgaatatt ccgattaaga ataaaaagtg
     4201 atttcctcca aatgccgcta gtactgttcc acttattgtt gctgtgaaca ttccgccaac
     4261 atatatttta ccaaatagtt tatggaaacg agcacccttt ttattgctaa tatttaataa
     4321 tccagttatc aatccaatca tacctcccgc tacatgcagg attaataaaa atgtgtgaag
     4381 cgttttcata atccttagtt tattagaaag taattcgata catcatcatt gggaatagtc
     4441 ctacctgata attattttca attttcttgg aagagctatt ataggaacgg ctaaacaaat
     4501 ttttatggtt tgttaaattc tgaaaatcaa gtccatattc ttctgtgatt ttctttttat
     4561 tcctgcgaaa tccgattcga atatccgttc ttaaatatcc tggtaattgc tcagaataag
     4621 ctttctcttc atttctaatt tcaaaaccca tttgttgtga tgcttccaaa ttaataggtg
     4681 tcatccacat tccacccgta acaacttgac gaaaactgaa taccaaaaca ttttgctttg
     4741 ttttcccaac tggaatttct tttccagcta ataaattaaa tgcatattta ttattaaatg
     4801 cagtatttcg ttcaatgcca tccgaacctt tgtatttact atcatacaaa ctggctgtca
     4861 tcaagaaata atagttttta tgaaagaatt tttccaatgt taactcaact cctttattaa
     4921 atccttttcc tttggctgtc aagctatctg gaccaatcac atcaccaaag tttgccccat
     4981 aattcaaaac cgataaacta ttcaatttac catcttgaac tcctacatta tattgttgct
     5041 gataatatac ttcagccttt aatcgtaaat gttcacgtat caaccaatca tatccagcaa
     5101 caaaatgatt ggaacgaagc aaatccaaat tcttatttgt ttgtatcata ttcccatcag
     5161 ctaattgagt ttctcttaaa taaatagtaa tcggtaatac tcggctatga ttaccataac
     5221 ctatactaat acgttgattt cctgttgctt gaaaactaat agccgctctt ggttctaatg
     5281 aactttgatt attcacgtaa taatgaagtg catgaattcc cgcatctaaa gtccaacgtt
     5341 caccagcttt gtagtgccac tgagcatatc cttgaactaa atctccattc ccgttaaaat
     5401 caatcaattg ttgacgtgca tttagtgaag cagaataccg attaacatca ctattataca
     5461 ttaaatgact aacaattaca cctgcacgaa atgtagtttt tgaattcaaa cggctattta
     5521 aaacagctgt tccggtgagt ttactttcct ccattttgct tctaaaatta tccagcattg
     5581 catatgaagg actgatagaa tcgatacttg tgcgaaagcg atttccagtg gcagctacag
     5641 tagttcgtaa actcgttttt gtatttaaga aatgtacatg agtcaaacca acaaccgcca
     5701 tatcagaacc attcttcaaa tcctgacgtt ttgctggatc tttcacccaa accaaactat
     5761 ctttttcgga ttcaaagtga atatcgcttg tacctgcaat tccaaataaa ctcgtatatc
     5821 cgtttttatg ctggaaatga agtttcatac ttatatcttg atactgagga actccacctt
     5881 taacaaattc aatcccaacc gcattaaatg cacctaaagt ggaataacga taattaatta
     5941 aatacgatga ttttttattt tttgataaag gaccttccgc tccaatttca aatccattaa
     6001 atccaatttg tcccgtgaat tcatgtttct cgttgttgcc ttttcgcatt ttaacatcga
     6061 atgctgcaga agtagcattt ccatattcgg ctgcataagc acctgataaa aagtcgctat
     6121 tcgctagaac gttagtgttt agaatagaaa cggctcctcc gcttgtcccg gcgccactat
     6181 aatggctcgg actaggaata tcaatacctt ctaatctcca caaaactcca agtggtgtat
     6241 ttcctcgaac aacaatgtcg ttacgttgat catttcctgc aacaactccg gcaaagctgg
     6301 tagctaatcg agcaggatct ccaaaactac cggcataacg ttgtgcttca tctactgaaa
     6361 atgttcgagc actcacgcct gctaattcat ttagtggttt gtctttatcg atcttagcta
     6421 caatctcaac ttccttccct tgaatagcag aagattccaa gttgatattt aggataagtt
     6481 ctttggtgga tgttaaaact agattgctga gtttaacggg ttgatatccc atcatgctga
     6541 tttgaattac cggtctacca acaggaacat ctattaagcg aaaatttcca tctaaatcgg
     6601 cgctaacacc ttttaatgga tttgatccaa ccaaaaccac agttgcaccg ggaattggac
     6661 tttgtgaagc tgcatcactc actgttcccc gaattgtctg agtgagttct tgagcagtta
     6721 ctttagttga tagactaaac aatgaagcca gaatgatagt aaactggact agtagaatgt
     6781 ttttattgat acgaatgaag cgaccttcaa aaatgtttgt tttcatatgc ttgtagaatt
     6841 gaaggcaaaa gtctttggaa tagacaatcc tacatataga attccgacga agcaacttat
     6901 ttgaaggatg aaccgatttc agaataggat aaaaaaacgg tccagtaaaa accggaccga
     6961 ataccactct taaatactgg gatcacaaac caccctgtgt aacaagacgg caaattacac
     7021 ctgagaaaag taagtgctta aattttgttt tcatatactc cttttggagt attttactga
     7081 tagaaacgga ataaaccgaa caaacagatt tttctagtat taggactgta taaaatgtag
     7141 aagtacccaa tagattggcg cagataatag tgtggaatcg aatcgatcta agattccacc
     7201 atgtccaggt aaaatggtgc ctgaatcttt gatgttagct tgtcgcttta acatcgactc
     7261 aaataagtca cccatagaac ctgtaacaga taaaacacct ccaataagca tccacattgg
     7321 tagagaagct cctcctaaaa agtagtaggc agcaatacca actaacatgg cagtaactat
     7381 tccaccaata aaaccctcaa tcgttttttt aggtgatagt cgttcgaaaa gcttgtgttt
     7441 tccaaatgcc cttccagtaa aataggcacc tgtatcagaa gcccacataa agaaaaacag
     7501 gtacaaaaca ttccaaggta aatagttgcc atcgtttcca attgagcagc taactaataa
     7561 gccacatggt actccaagat aaataagact tagaatagag cttgctaaat tttctagtgg
     7621 tttgggaata ccacgaaaca actcccacgc aaaaaggaga aatagtaatc caataaaaat
     7681 agataagaat agaaatgtat catgctcaac taatccaatt gaatgttgag tcatggagaa
     7741 atagatgcaa gctgcaaata caagaatatt agctaggtat aagaatgccc ggtttgtatt
     7801 aatagacaaa gcatgaacaa gtcgattaaa ctcaaaacac ccaacaagta atatgaataa
     7861 agctatgctt gtaagtgcat attcggataa aagtattcca ccaattaaaa ctatcccgaa
     7921 aatagctcca gttataacac gggtttgatg attcgataaa gccataatgc tacattaatc
     7981 ttgaaaagta tcaggtgatt cattctgagc ttcatgtata atttgaagcg ctttcaaacg
     8041 aaatgctgca tccacataca attctgcttc gccaatactt ggatataccg aatctacttt
     8101 atttagaatc tgggcaggaa tgtctttttg ctccattaaa ttgagtaaca tgtacaaacg
     8161 agatacattc gtgtcgcttg caattactgt ccattgattc tccatgctgc aaattacatt
     8221 aatcctataa aacacgaaag gacaacctaa gctgtccttt catgaaaaaa atataataag
     8281 tgattatgct tcctccgaag gattagttct agcttcttct tcttttgaaa cagttgaggt
     8341 atcgggtttt gataaattac tcggttcaat tttctcttcc gatttttctt ttgctgcatt
     8401 agctgctgca atgtcctgat tgtcttttaa ctgatctttg tatgaacgag aaatatctga
     8461 actagcactt tcatcatctt tatcccagat acgttttcca aaaatctctt ctaaatcttc
     8521 acggaaaata acctcacgtt ctaaaaggat tgtagctaaa gaatccaatt tagttttgtt
     8581 ttcctgaagg atcttcttgg tacgttcata tgccatatca atcaacttct tcacttcctg
     8641 atcgatcgtt tcagcagttt tttcgctgta tggacggttg aagctatatt ctgattgtcc
     8701 ggatgaatca tagaaactta tgtttccgat tttatcgttc aaaccataaa tagtcaccat
     8761 tgcatatgct tgtttggtga ttttttctaa atcgctaagt gctccggtag agatttttcc
     8821 aaatgtcact tcttcagctg cacgaccacc taatgcggcg cacatttcat ccaacaattg
     8881 ctcaaacgta gtaatcgagc gctcttcagg aagataccat gcagcaccta aagattgacc
     8941 acgtggtaca attgtaactt tcacaagtgg tgaagcgtgt tctaacaacc aacttaccga
     9001 tgcatgtccg gcttcgtgga aggcaattac gcgcttctcg tccattgata ttatcttgtt
     9061 ctttttctcc aaacctccaa tgatacgatc tactgcatct aagaaatctt gcttgccaac
     9121 cacatctttg tttttacgag ctgcaatcaa agccgcttcg ttacaaacgt ttgcgatatc
     9181 agcacccgag aatccaggtg tttggcgagc taagaattcg atgtctaaac tttgatccgt
     9241 cttaatcggc ttcaaatgca ctttgaatat ttcgcgacga ccatttaaat caggaagttc
     9301 cacataaatc tggcgatcga aacgtcccgg acgaagtaag gcacggtcca aaatatcggc
     9361 acggttagtt gctgccaaaa tgattacacc attattggtt ccgaaaccat ccatctctgt
     9421 aagcaactga ttaagcgtat tttcacgttc atcgtttgct ccagccattg gatttttacc
     9481 acgtgcacga ccaatcgcat caatctcatc aataaatatg atggcaggcg ctttctcttt
     9541 cgcttgtttg aaaagatcac gtacacgact agcaccaact cccacaaaca tttccacgaa
     9601 atcggaaccc gaaagggaga aaaacggtac ttgagcttct cctgcaacag ctttagccaa
     9661 taaggtttta cccgttcccg gagggccaac taataaagca ccacgaggaa ttttacctcc
     9721 taaaatggtg tattttttag ggtttttaag gaagtcaaca atctcacgaa tctcaacttt
     9781 tgcttcatct aaacctgcca cattgtcgaa tgtaacggta acatgtgtgt ctttatcaaa
     9841 cagctgagcg cgactttttc caatattgaa aatttgtccg ccgccgccgc cgccgcccat
     9901 tctacgaata agtagcatcc aaaataggaa tacgacacca agtaaaatca cccaactaat
     9961 gatttcaccg gaccagtttt tacgactctc gtaactagga tgaattcgga gttcaggagc
    10021 aacgctaact tgagcagtat cgatgatttt atttaaatcc tcaaccgatc caatggtgta
    10081 attgtaatgt ggcccttttt ctaaagaagc cgacatattg ggcgatttga ggtttttata
    10141 ttcgggtttg ctaagctttt ctttcttgat tgttatctca gcatattctt tattcaccac
    10201 cgttatttcc ttcacatcag ctgcaccgag catttcagtg aattctttgg ttttgatttc
    10261 acgtgtgttg gttccaacat tatagaaata ggaaccaata aatagcaaag tgattatccc
    10321 ataaatccaa taaaaattgg atccgcctcc actagaaggc ttgggtttac cagattgagg
    10381 aaaaagtcta cgattgccgg agtttttggg tgtacccgga gtattatttt gaggttgttc
    10441 agccattgga aaattcaaag cgtgattgca tgttgaaaaa gaacacgatt atcacagata
    10501 agtttatcag gcaacagatt cagggataac tcggacaata gcatctgccc aaagtcgttc
    10561 gattttgtag aaatctcgaa tagaagtctg gaatacatgc acaaccacat tcacgtagtc
    10621 gatcaatatc cattcttgat tttcgaaacc ttcgctacgc caaggagatt ctcctgttgc
    10681 aactttaact tcatgttcta ccgagtatgc gattgcatct acctgagtgc ctgaatcggc
    10741 attacagatg acaaaaaaat cgcagaccga atgtcctgtc tgccgcagat cgaagacctg
    10801 gatgttttta cctttctttt cttcgattcc tttgatgatc gggtcgatca gtgcttcagc
    10861 ggagtcagtg gcaatagatg ctgccttctt tttcattcag atgcttgatt gttgcgacaa
    10921 atttagtatt tctgccgatg atggaactat tatttatggg ccgtaacctg atccggttgg
    10981 acagctgcgc gtcgactaac aactttgctg ccagtttgct tgagcaaaat gctccagaag
    11041 ggacgcttgt tgtagcaaaa gaacaaacgg gtggacgggg acaacgtgga aaaacatggt
    11101 atgctgagcc gggcaaaaat ctcacgttaa cgtatatact tcgcccatta tcattgagga
    11161 ttgctgacca atttctgcta aataagacaa ttgcaattgc tgtagctaga acggtcgaac
    11221 tcattagtcc tggatctttt attcgcatta aatggccgaa tgatattttt cttgagaata
    11281 gaaagttggc aggtatctta gtcgaaacgg cggtcaaggg aagccagatt gtcagttgtc
    11341 tggcggggat tggaatcaat gtcaatcaga cagtttttcc agatgatagt gggaatccag
    11401 tttcattagc gatggagctt gaaacacagc ttgttttaga tgatgtcatg atgcttttga
    11461 gcaagcaaat ggaagtgtat tattttcgat ttagctcaag ccacaaagcc ttgttagaag
    11521 aagaatacga ttccttgtta tggaaacgag gtgaagttta tccgtttaaa tttaatagtg
    11581 aagatttccc tgccgaaata gtaaatgtag atgagcaagg gaaactagta ttgaaaacaa
    11641 ccgagggtga agaaagacgc ttctcacatg gagaaataga ctggttctaa tcgattaagg
    11701 cttattttgt tcgataccaa aatcgattcg aaccgatttg ataaacgttt ctttttcgat
    11761 ggtgaagtag gtttcgttga caagttccat ttcattcatc gatttccctg agctagtagc
    11821 ataataatca actcctcctg ctccgtttcc tgagccatat ccttcattta cctcataaaa
    11881 aacttgctgc acttgagcga gcttaacttt taatgaagtt gcaatgatgg ttgccttttc
    11941 ggatgcatct gtaattgcat gttcgatgag ttttttctct actgcttttt gcaaactatc
    12001 ggaacaaaca gagttgtagg taatatacgt ttgacgagca tcttcggttg aaaatgcttt
    12061 caatgttttt agaatgattt ctttactcaa acgtgtttta acggtcagat tttgagtggc
    12121 tcgatatccg ttacgttttg ctttatcgcc tacatactca taattggcta caaccgaata
    12181 gtcgtttaat gtgatttcct tttcgggata accaagctct ttaagttttg ttaaaacgtt
    12241 attcgaaacg accgttaaat cttgaatact caaactttct gatttgttcc gtgcattcaa
    12301 gtgaagttta aaaacggcta tgtcgggtgt tacttccatg tttgctacac ctgtgacctt
    12361 gatagctggt aattcctggg aatgaagatt tataaagaga atactcgcta ttactgtgca
    12421 aatgaatttc atacgaagaa gttttcttat gaatgtaaaa tgacagcaaa ttattgcgcc
    12481 acaccattta gaatcactgt tttcaccaag tcggttccaa aggcgtaggg tagaaaagca
    12541 aacgatggta ttttagatgt gataaagagg ttcgctactt ttcccggtgt gatgctgcca
    12601 tgcgtttcgt gcaatcccat agcataggcc gaattgattg ttgcagcatt gatagcttct
    12661 tcgggcgtca tacgtaagtt gatacaagct aagctgagca caaattgcat tcgtcccgaa
    12721 ggagatgaac ccggattgta atcggttgct aatgcgatag gtaaaccagc tgcaatcatt
    12781 tcacgagcag gtggttgttt tatttttaag aagaatgctg tggatgggag cagagtaggc
    12841 attgtgccac tattgagcaa tacttccatt tcttcttcac cagtaaattc taaatgatcc
    12901 acacttaatg ctccatgttt tactcctact tgaattcctc ccgaataatc aagctcgttt
    12961 gcatggattt tagcacgaag cccaagtcga ttggcagctc ctaaaatgcg atcggtttcg
    13021 tcgacggtga agaagccttt atcgcaaaac acatcaacga aatcggctaa accttcggaa
    13081 acaactttcg gtaacatttt ctctacaatc agatcgatgt aggctgaacg attttctttg
    13141 tattcggtag gaagtgcatg tgcacctaag aatgtggctc gaatggttaa atctgtagct
    13201 tctcgtaatc gtcgaatgac acgtagcatt tttaattcat cgtcgaccgt aagtccataa
    13261 ccacttttta tctccacagc accggttcca tatccttgaa tttcgatagc tcgtgttaaa
    13321 gcctgttcga aaagatcatc ttcgtatgtt gcctgtaagc gacgtgcaga attcaaaatt
    13381 ccaccaccac gacgagcgat ttcttcgtag ctcaaaccgt gaattttatc gacaaattcc
    13441 gactcacgac taccagcata aacgatatgt gtatgactat cgcaccaaga tggaaacaca
    13501 aatcgttaat gctggtagtc gtgagtcgga atttgtcgat aaaattc
//
LOCUS       DAOOQW010000045        13344 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_5705_length_13344_cov_5.519452, whole genome shotgun sequence.
ACCESSION   DAOOQW010000045 DAOOQW010000000
VERSION     DAOOQW010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 13344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13344
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5705_length_13344_cov_5.519452"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(81..506)
                     /locus_tag="PK281_04390"
     CDS             complement(81..506)
                     /locus_tag="PK281_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020211522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HOY48615.1"
                     /translation="MTEQELQETFDEQGHHISPVLPTSVINYLIDIDGTICDDVPNEE
                     PERMVTAEPYPDALETLNKWYDEGHIITFFTSRTEDLRDITEEWLNRHGFKYHGILLG
                     KPRGGNYHWIDNHIVRGTRFKGKFTEMETVMKEILVFKK"
     gene            complement(1040..1432)
                     /locus_tag="PK281_04395"
     CDS             complement(1040..1432)
                     /locus_tag="PK281_04395"
                     /inference="COORDINATES: protein motif:HMM:NF013633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HOY48616.1"
                     /translation="MGVRIDKYLWAVRIFKTRSMAIDAIKGGKVKLEGDNVKPARDVK
                     EGETYTIQLSQIQKTIRVKALLENRVAAKDVNLYMEDLTPESEYKSIDRMKETRFVWR
                     DRGTGRPTKKERRDIEEWFDEDSEKKVE"
     gene            complement(1432..2922)
                     /locus_tag="PK281_04400"
     CDS             complement(1432..2922)
                     /locus_tag="PK281_04400"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018944451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HOY48617.1"
                     /translation="MKKILVANRGEIALRVMRSAREMGIQTVAVFSEADRNSPHVRYA
                     DEAVCIGPAASSQSYLRGDKILEVCRELGVDGIHPGYGFLSENAGFARAVKEAGITFI
                     GPSPEAMEMMGSKLAAKAAAKAYNIPLVPGTEEAISDVDAARKIAEEIGFPILIKASA
                     GGGGKGMRIVENSGEFNEQLQLAISEATSAFGDGSVFIERFVTSPRHIEIQVMSDTHG
                     NCVYLFERECSIQRRHQKVIEEAPSAILSPELRKAMGECAVLVAKACDYVGAGTVEFL
                     VDDKLNFYFLEMNTRLQVEHPVTELISGKDLVKEQIRVARGEKLSFTQDELTINGHAL
                     EIRVYAEDPANNFLPDIGNLSVYQLPIGNGVRVDGGFEEGMDIPIYYDPMISKLIVHA
                     ENREAAIAKMIRAIDDYQIAGIETTLSFCRFALQHEAFVSGNFDTNFVKHYFKPELLE
                     RPATEDELEIAALFAAWDRTQQISATPAIAPQATTSKSAWKERRYN"
     gene            complement(2919..5861)
                     /locus_tag="PK281_04405"
     CDS             complement(2919..5861)
                     /locus_tag="PK281_04405"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOY48618.1"
                     /translation="MKRILFLLFFGLLLNLVNAQSNNIPGEFLVQLKGKHSAALIERN
                     MAINHPEWEMKATRLLSARFHIWLFTSLPGDDAEKLSYLSKDSKVVLAQHNHQVQLRS
                     TVPNDPSFTQQWALNNTAQSGGTAGADIQAEDAWDITTGGLTTSGDTIVVAVIDGGFQ
                     LNHPDLVQNIFVNRLEIPGNSIDDDGNGYVDDVNGWNAYDDNGTIPSDQHGTHVSGII
                     GARGNNNIGIAGVNWAAKILPIAGSSGDEATVVAAYAYAAEMRIRYNESNGLEGAYVV
                     STNASFGVDQGDPADYPIWCSFYDTLGTYGILNAGATANANFNIDQTGDIPTACVSQF
                     LIAVTNSTNADVKYNSAGYGVVSIDIASPGTAIYSTVTNSNYGNLTGTSMATPHVAGA
                     IGLMYGAACEELIAESKSNPASVALAMRNYLLDGAEQLSSLNNLVDGSRRLNLLGALQ
                     QVQSYICDPTAPPSANFSAPAPSRNGCPGLSVTFLNQSSANANSYSWEFPGGNPATST
                     EENPVVVYNSVGVFPVQLIANNDFGADTIQFPNYVDVNTSGTRFVLNETFDAATFTEA
                     GWSTVNPDNASTWILATVGGSSPGTQAAEIPIFANQAAIGQRDGLISPELSLLETSSN
                     SLYFEHAHRRRVTSVRDSLILAVSTDQGANWTRIAAYAESGQGSFATGNLTTSNFVPT
                     ANINWCTASTTGPDCFTLDISAWDGAPSFLVKFEAYNAGGNNIFIDNVKVSGICSNPV
                     IVQPQAAFAINTDPTCTNQPIQFTNSSTDATSYSWSFPGAFPSTSTEISPTVLYAAAG
                     TYSVQLIASNSQYSDTLDMQSFVTVQESPAPPLLTFGSETISTAAVGSFQWLFNGVVI
                     PNATTSQLTPTQSGDYTCVVTSPNGCSASSTMNIIIDGLEQLANSNLSIYPNPVEHDL
                     TIKLNSPETALCTLFDASGRKLISSTVVSSGIIDMKEYPAGVYILSVIVNGQQSHHKI
                     IHTSALAH"
     gene            complement(5954..7144)
                     /gene="sucC"
                     /locus_tag="PK281_04410"
     CDS             complement(5954..7144)
                     /gene="sucC"
                     /locus_tag="PK281_04410"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014780847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HOY48619.1"
                     /translation="MNIHEYQGKSILKRFGVGIQEGIVAETPEEAVEAAKLLQQQTGT
                     GWWVIKAQIHAGGRGKGGGVKLAKNLDEVRSISDQILGMTLVTPQTGPEGKFVRKVLI
                     AQDVYYPGPEPTKEFYMSVLLDRQTGRNIIIYTTEGGMDIEEVAHSTPEKILKEEIDP
                     RVGLQGFQARKIAYNLGLSGEANKQMVKFVTSLYKAYDSIDASQFEINPVLKTSDSKI
                     IAVDAKVNLDENGLFRHPDILEMRDTTEEDPTEVEAGEHNLNYVKLDGNIGCMVNGAG
                     LAMATMDIIKLAGGDPANFLDVGGTADAARVEQAFRIILKDENVKAILVNIFGGIVRC
                     DRVAQGIVDAYKNIGEIRVPIIVRLQGTNAVEAKKIIDDSGLRVKSAILLQEAADSVK
                     EVLS"
     gene            7333..7896
                     /locus_tag="PK281_04415"
     CDS             7333..7896
                     /locus_tag="PK281_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48620.1"
                     /translation="MFRESTISAQEISSISQRILGKTMQMWMDILRAQKIQPPTKQKL
                     IHFLSTNYMMDEADAEMISLRYFMLQASDAGSIYTDGYEITVTRTYPVGLHVLESYFT
                     EKNLRELHLLADLQLIKYNPGKNIRFQLANGEILLANFNGKSAGKCQVTLQHTKLADS
                     DKSEEARSFWKERLQMLADLFTGNDKS"
     gene            7883..>8282
                     /locus_tag="PK281_04420"
     CDS             7883..>8282
                     /locus_tag="PK281_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014034356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOY48621.1"
                     /translation="MIKAKSITKSFGELQVLKGVDLEIERGEVVSIVGASGAGKTTFL
                     QILGTLERPDGGELDFDGEKPTLLNDRKLSAFRNQQIGFIFQFHQLLPEFSSLENVMI
                     PGMIAGTSRSELEIRAKELLDQLGLKDRMDS"
     assembly_gap    8283..8382
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8383..8697
                     /locus_tag="PK281_04425"
     CDS             <8383..8697
                     /locus_tag="PK281_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOY48622.1"
                     /translation="IRAKELLDQLGLKDRMDSKPSTLSGGEQQRVAVARALMNKPKLL
                     LADEPSGNLDSKNARELHELFFQLRKDLGQTIVLVTHNEDLARMADRRLVMVDGRFQQ
                     EG"
     gene            8820..10613
                     /locus_tag="PK281_04430"
     CDS             8820..10613
                     /locus_tag="PK281_04430"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HOY48623.1"
                     /translation="MSTAQIQETAEAVLQRYFGYSEFRPLQEEIISQVLSNTDCLVLM
                     PTGGGKSVCFQVPALMKAGTALVISPLLSLMKDQVDALKANGIQAATWNSGTSPEEEF
                     DIIEFCKNGKLKLLYISPERLQVLKNSFMSEIHWSLFAVDEAHCISSWGHDFRPEYTR
                     LNGLKELYPDVPIIALTATADLSTRKDIVEQLKLRNARQFIASFDRPNLSLDVRGDMS
                     KDIQDKEILAFIRKRRNQCGIIYCSSRKKTEMLSHFLNRQGIRSEHYHAGMVYPDRVR
                     VQENFLYDRTQVICATIAFGMGIDKSDVRYVIHYNLPRSVESYYQEIGRAGRDGLPAE
                     TLLYYHHSDFRLQRKYALESGQVELNLEKLRLLKHYTEGTACRRKALLNYFHEAYPRE
                     CGNCDRCRARNTNQHLAESEVKQPNLQGLLEHLRQVRLQLARQEKLPAYIIFHESTLK
                     EMSELRPDQLPGICQVHGMSENKFRKYGKYFFLEIAQWYRNQGYSLPNESLLQTWLLL
                     REGKSPEDVAVQRALSLSTIFQHAGILIQAEYPLEQEAFLCSEKRKQIEKAVNVLGYT
                     GKRKPIVDYFNGALSYHELSFYLDSQKQISV"
     gene            complement(10603..12756)
                     /locus_tag="PK281_04435"
     CDS             complement(10603..12756)
                     /locus_tag="PK281_04435"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HOY48624.1"
                     /translation="MRVLSLLLSFTLFSVRLLADEGMWLPMLLKQLNEKDMKKMGMKI
                     SAEDIYSVNKSSMKDGVVLFGRGCTGEVISKEGLLLTNHHCGFGQIQDHSSLDHDYLK
                     DGFWAKNHADELPNPGLTVTFIIRMEDVTAKMIEGLDKITDESERENRITQRSMELVK
                     EAVANTHYEALIKPMYNGNEYVLFVTEVFRDVRLVGAPPQSIGNFGKDSDNWVWPRHT
                     ADFSLFRIYAGKDNKPADYSPDNVPFQPRYHFPISTSGIKENDFTMVYGFPARTNEYL
                     TSNAVDQIVNLSNPLKVDLRGQRLDVMNKYMSADRGVFIKYADKYSGVSNYHKKWAGE
                     AKGLMRAHAIDKKKEFESKFQSRVDADPKFSAYKNLLSDFNREYATLKDIQPELDLYN
                     EALLTIEILRFANGFRELTNGSIDLKSEKGKEILANQKKRVAEFFKDYYSLLDREIMT
                     AMLKSYYSKSVKPVSPDFIAGLSAQATTNDVEVVTTIVFDSSFLDDEQAVSKLLENPD
                     SAAIAKLKGDIAYKLAVFYFDQYRNVVLKNYNGVMARLIPMNRLYVKAQREVIPEKRY
                     YPDANQSLRVAYGKTEGYSPADGVNYTWFTTADGLLQKSNSGVVDYSIPERLKDLIQK
                     KDFGPYADKDGQLHTCFAASNHTTGGNSGSPVFNAKGQLIGTNFDRNWEGTMSDVYYD
                     INQVRNITADIRYTLFIIDKFAGATHLVNEMTIIH"
     gene            12956..>13344
                     /locus_tag="PK281_04440"
     CDS             12956..>13344
                     /locus_tag="PK281_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016650108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48625.1"
                     /translation="MSIQIQVPQPCHASWDKMTPQENGRHCKSCDKVVIDFSRMNDEE
                     IKKWFRKKEGEKICGHFRVDQVKRLQIVVKPREFKQLGWSPVQLIRVAIFLVFFSSLF
                     SCSPYPEQNGAMTEIVIDDEKDPNDEI"
BASE COUNT         3901 a         2656 c         2799 g         3888 t
ORIGIN      
        1 cgtatatatt gagtaacaat cccctttttg atccgaaagt tgctttgcag gaagattgga
       61 aaaaactgga aaagaagtaa ttacttctta aataccagaa tttccttcat aacagtttcc
      121 atctcggtaa acttgccttt gaatcgtgtt ccgcgaacga tgtgattatc gatccaatgg
      181 tagtttccgc cacgtggttt acccaacaaa attccgtgat atttaaatcc gtgacgattg
      241 agccattctt cggtaatgtc gcgtaaatct tcggtgcgtg aagtaaaaaa cgtgatgata
      301 tgaccttcgt cgtaccattt attgagcgtt tccaaagcat ctgggtacgg ctcagcagta
      361 accatacgtt caggttcttc attcggcaca tcgtcgcaaa tggttccgtc gatgtcgatt
      421 aggtaattaa tcacggaagt tggcaatacc ggactgatat gatgtccttg ctcgtcgaaa
      481 gtttcttgca gctcttgctc agtcatggct tttgcatttt atgcaaagat aacgctcgaa
      541 ctataactat tatgtaaacg aattagattt tttctgaagt tttatacacg acaatgttga
      601 aatacccgac aattgagcaa taagcgtgtt aataagacac tttttgatgt gctggtgtgt
      661 tagtttgctg atgtgctcct ttcgacaagc tcaaggagcg tgatgtagag cgctgtgctt
      721 ttgtcgaagc gtgttgttta gatttcgttc cgcttgctga gcctgtcgaa gcaagctttg
      781 attaatgtgc tgatatgcta gtgtgctgat gtgctgttta gatttcgtca gactggagct
      841 acttggttag tcttctaaat gattttgatt tgaaaatttg aagatttgaa aatttgaaaa
      901 tgagcttcac gttaaacttg aatcgcttgt tgagcctgtc aaaggaagct agttagtatg
      961 tttcaaaaat cgtaaatcgt aaatcgtaat tcgtaattcg tatttcgtaa ttcgtatatc
     1021 gtatatcgta aatcgtaaat cattccactt tcttctccga atcttcgtca aaccactctt
     1081 caatatcgcg gcgttctttt ttagtggggc gaccggtacc tcgatcacgc cagacgaagc
     1141 gagtttcctt catccgatcg atgcttttgt attcggattc gggtgtgaga tcttccatgt
     1201 atagattcac atctttggca gctacacggt tttcgagtag tgcttttacc cgaatagttt
     1261 tctgaatttg ggaaagttga atggtatacg tctccccttc cttcacatcg cgggcaggtt
     1321 taacgttatc gccttcgagc tttacttttc cacctttgat tgcatcaata gccatggaac
     1381 gggtcttgaa aatccgaacc gcccagaggt atttatcaat ccgaactccc attagttgta
     1441 gcgacgttct ttccaggccg atttactcgt tgttgcttga ggagcaattg ctggagttgc
     1501 gctaatttgt tgggtacgat cccaagcggc aaacaaagct gctatttcga gttcatcttc
     1561 agttgctgga cgttcgagca attcaggttt gaaataatgc ttcacgaaat tggtatcgaa
     1621 attcccagat acaaaggctt cgtgttgaag cgcaaagcgg caaaatgaaa gcgtggtttc
     1681 gattccggca atttgataat catcaatggc gcgaatcatt ttagcaatag cagcttcgcg
     1741 attctcagca tgaacgatta gtttgctaat catcggatcg tagtagattg gaatatccat
     1801 tccttcctca aatccgccat ctacgcgcac tccgttacct attggaagct gataaacaga
     1861 aagatttcca atatcaggta ggaagttatt tgctggatct tcggcataca cgcgaatttc
     1921 gagcgcatgt ccatttatcg ttaactcatc ttgcgtgaaa cttaatttct ccccgcgagc
     1981 aactcgaatt tgttctttga ctaaatcttt cccagagatt aattcagtta ccggatgttc
     2041 aacctgtaat cgggtattca tttcgaggaa gtagaaattg agtttatcat caactaaaaa
     2101 ctcaactgta ccagctccca cataatcgca agccttagct accagaacgg cacattcgcc
     2161 catggcttta cgtaattcag gacttaaaat agcggatggt gcttcttcga ttaccttttg
     2221 atggcgacgt tggatgctac attctcgttc gaataaatac acacagtttc catgtgtatc
     2281 ggacattacc tgaatttcga tgtgacgagg agatgtcacg aaacgttcga taaatactga
     2341 accatctcca aaagcagaag ttgcttcaga aatagccaat tgcaattgct cgttgaattc
     2401 accactattt tcaacgatac gcattccttt tcctccacca ccagcagagg ctttgatcaa
     2461 gattgggaat ccgatttctt cagcaatttt acgtgcggca tctacatccg aaatagcttc
     2521 ttcggttccc ggaacaagag gaatattgta ggctttagca gcagctttag cagctagttt
     2581 agaacccatc atttccattg cttcgggtga tggtccaata aaagtgattc cagcttcttt
     2641 caccgcacga gcaaaaccag cattttcgga aagaaatccg taaccgggat gaatgccatc
     2701 cacacctaat tcacgacaaa cttctaagat tttatcaccc cgtaaatagg attgactaga
     2761 agctgcagga ccaatgcaga ctgcttcatc ggcataacgc acatgaggtg aattacgatc
     2821 ggcttcggag aaaaccgcta ctgtttgaat tcccatttcg cgtgccgaac gcatgacacg
     2881 cagggcaatt tcgccccggt tggctacgag tatctttttc aatgtgctaa agcgcttgtg
     2941 tgaatgattt tgtgatgaga ttgttgtccg tttacaataa cagaaagtat gtaaactcct
     3001 gccggatact ctttcatatc gattattcca gaagaaacaa cggtgctaga aatgagttta
     3061 cgccctgagg catcgaataa agtacacagt gccgtttcgg gtgaattcaa cttgattgtt
     3121 aaatcgtgtt ccaccggatt tggataaata ctcagattcg aattggctag ttgttccaat
     3181 ccatcaataa taatgttcat ggtacttgaa gcagaacagc cgttaggaga agtcacgaca
     3241 caggtataat caccactttg ggtaggagtc aattgtgaag ttgttgcatt aggaattact
     3301 acaccattaa ataaccactg aaacgaacca acagcagcgg tcgatattgt ttcagaacca
     3361 aaagtcaaaa gaggtggggc tggagattct tgaacggtaa caaacgattg catgtcgagc
     3421 gtatcggaat actgactatt ggaagcaata agttgaactg agtaagtacc tgcagctgca
     3481 tataaaactg ttggacttat ttctgtggat gttgatggaa aagctcccgg aaaactccaa
     3541 gaatatgaag tagcatcagt acttgaatta gtaaactgaa taggctgatt ggtacaagtt
     3601 ggatcagtat tgatggcgaa agcagcttgt ggttgaacaa ttactgggtt tgaacagatt
     3661 ccagaaactt tcacattatc aataaagatg ttatttccac ctgcgttata ggcttcaaac
     3721 ttcactaaaa atgaaggagc gccatcccaa gcagaaatat ctagagtgaa gcaatcaggc
     3781 ccggtggttg aagctgtaca ccagttaata ttggcagttg ggacaaaatt ggaggtagtt
     3841 aaatttccgg ttgcaaatga tccctgacca ctttctgcat aagctgcaat gcgcgtccag
     3901 tttgcacctt gatcggttga aacagctaag attaatgaat cgcgaacgga agtaactcgg
     3961 cgacgatgtg catgttcgaa atacagggaa ttagaactgg tttcaagtaa tgatagttcg
     4021 ggactaatta atccgtcacg ttgtccgatt gcagcttggt ttgcgaagat tggaatttca
     4081 gccgcttgtg ttccgggtga tgaacctcca acagtagcta atatccaagt actagcatta
     4141 tcgggattaa cggttgacca accagcttcg gtaaatgtag cggcatcgaa tgtttcattt
     4201 aatacgaatc gggtaccgga tgtatttaca tctacatagt ttggaaattg aatcgtatca
     4261 gcaccgaaat cattgtttgc aattagttga acaggaaaaa ctcctacaga attatagact
     4321 acaactggat tttcttcagt agaagttgct ggatttcctc cagggaattc ccagctataa
     4381 ctgttcgcat tagcacttga ttgattgagg aatgttacag aaagacccgg acaaccatta
     4441 cgtgatggag ctggggcaga gaagttggca gaaggtggtg ctgttggatc gcagatatag
     4501 gattgaactt gttgaagcgc gcctaacaaa ttcaagcggc gactaccatc tactagatta
     4561 tttaaagagc taagttgttc tgcaccatcg agtaaataat tacgcatggc aagtgcgaca
     4621 gaggctgggt tggatttgga ttcagcaatt aattcttcac atgctgcacc atacattaaa
     4681 ccaatcgcac ctgcaacatg aggagttgcc atggaagttc cggtcaaatt cccataattt
     4741 gagttggtaa ccgttgaata gatagctgtt ccgggtgatg cgatatctat agaaacaaca
     4801 ccataaccag cactgttata ttttacatct gcattagtag aattcgtaac agcaatgagg
     4861 aattgagaaa cgcaagctgt aggaatatca cccgtttgat cgatgttaaa gtttgcattg
     4921 gcagttgcgc cagcatttaa gattccgtaa gtaccaagcg tgtcgtaaaa tgaacaccaa
     4981 atgggataat cagcaggatc gccttggtca acaccgaaag acgcattcgt agaaacgaca
     5041 taagcacctt ccagaccgtt tgactcgttg taacgaatac gcatttcggc agcataagca
     5101 taagcagcta caactgttgc ttcatcgcca gaagaaccag caataggtag gattttagca
     5161 gcccagttta caccagcaat acctatatta ttattacctc ttgccccgat aatacccgaa
     5221 acgtgtgtcc cgtgttggtc ggatggaatt gttccattgt cgtcgtatgc attccaacca
     5281 tttacatcat ctacataacc attcccatca tcatctatcg aattcccagg gatttctaaa
     5341 cgattaacga agatattctg cactaaatct ggatgattca attgaaaacc accatcaata
     5401 actgccacaa caatggtatc acccgaagta gttaatccac cggtagtgat atcccaagca
     5461 tcttcagcct gaatgtcggc tccggcagta ccgcctgatt gagctgtatt atttaatgcc
     5521 cattgttggg tgaaggatgg atcattagga acggtagaac gaagttgaac ttggtgatta
     5581 tgttgagcta aaactacttt cgaatcttta cttaagtagc ttaatttttc agcatcatcc
     5641 ccaggcaaac tggtaaaaag ccaaatatga aaacgagccg ataaaagacg agtggctttc
     5701 atttcccatt caggatgatt gatagccatg ttgcgttcaa ttagagccgc agaatgtttc
     5761 cctttaagtt gaactagaaa ttctccggga atgttattcg actgggcatt tacaagattg
     5821 agtaaaagtc cgaaaaagag taaaaataga attcgcttca tgtgatggtt tgaagtggca
     5881 agttaccaaa gaaatcgcct agtagaaact aaaaaaggcg cacaagggcg cctttctggg
     5941 taaattttac agattaggaa agaacttctt tcacgctatc ggcagcttct tgaagaagga
     6001 ttgctgattt aacgcgtagt ccagaatcat cgatgatttt tttcgcttcc acggcattag
     6061 taccctgaag tcgaacaatg attggaacac gaatttctcc gatgtttttg tatgcatcaa
     6121 caataccttg agcaacgcgg tcgcaacgaa caattccacc gaaaatattc accagaatag
     6181 cttttacatt ttcatctttt aggatgatac ggaaagcttg ttctacacga gcagcatcgg
     6241 cagttccacc aacatcaagg aagttagcag gatcgcctcc agctaatttg atgatatcca
     6301 tcgtagccat tgctaaacca gcaccattca ccatgcaacc aatgtttcca tccaatttca
     6361 catagttcag gttgtgttca cctgcttcta cttctgttgg atcttcttcg gttgtatcgc
     6421 gcatttcgag aatgtcgggg tgacggaaca atccgttttc gtcgagattc acttttgcat
     6481 ccacagcgat gattttgctg tcggacgttt tcaacactgg attaatctca aactgtgaag
     6541 catcaataga atcgtaagct ttataaagtg aggtaacaaa cttcaccatt tgcttattgg
     6601 cttcacctga aagtccaagg ttataagcaa tttttcttgc ttgaaaacct tgcaagccaa
     6661 cgcgaggatc gatttcttct tttaggattt tctccggagt gctatgagcc acttcttcga
     6721 tatccatacc accttcggtc gtatatatga tgatgttacg acccgtttga cgatcaagta
     6781 gcacactcat atagaattcc ttggtaggct ctggacctgg ataataaacg tcctgtgcga
     6841 ttaacacctt acgaacgaac ttcccttctg gacctgtttg aggagttacg agcgtcattc
     6901 caagaatctg gtcagagata gatcgaactt cgtctaaatt tttggcaagt ttcactccac
     6961 cacctttacc gcgaccacca gcatggattt gggctttgat aacccaccaa cctgtaccgg
     7021 tttgttgttg cagtaatttt gcggcttcga ccgcttcctc aggagtttcg gcaactatac
     7081 cctcctgaat accaacgcca aagcgtttaa gaatagattt accctggtat tcgtgaatgt
     7141 tcatgtgagc ttgaatttgc tggcaaaagt agtcgtttta atgaagcaag aaacaggaat
     7201 ctgtagctct ttttttatct gggtattact acctagttgc taacatttag gtgtgaatac
     7261 tattttcatg taactattat ttaccaacac acgtttgggg ctacattagt gtttcacaaa
     7321 ccaccaatat ctatgttccg agaatctacc atctcggcac aggaaatttc cagtatttca
     7381 caacgcatct tggggaaaac gatgcaaatg tggatggaca ttctccgtgc ccaaaaaatt
     7441 caacctccaa ctaaacagaa gttaatacac ttcttatcta ccaattacat gatggatgag
     7501 gctgatgcag aaatgatcag tcttagatac ttcatgttac aagcttcaga tgctggtagt
     7561 atttacaccg acggttatga aattacagta acccgaacgt atccagtagg acttcatgtt
     7621 ttggagagtt acttcaccga gaaaaacctc cgagagcttc atttacttgc tgatttgcaa
     7681 cttatcaagt acaatccggg gaagaacatt cgttttcaat tagctaatgg ggaaatattg
     7741 ttagccaatt ttaacggaaa gagcgctgga aaatgtcagg taacgttaca acatactaaa
     7801 ttagccgact cagataagtc agaagaagca cgttcgtttt ggaaagaacg attgcaaatg
     7861 ctggccgatt tgtttaccgg caatgataaa agctaaatcg attactaaat cttttggtga
     7921 actacaagtt ctcaaaggag tagatcttga aattgaacgg ggggaagttg tctctatcgt
     7981 tggtgcctca ggtgctggca agacaacttt cctccaaatt ttaggcaccc tagaacgccc
     8041 cgacggaggt gaattagatt ttgatgggga gaagcctacc ttattaaacg atcggaaact
     8101 ttctgcgttt cggaatcagc agattggatt catattccag tttcaccaat tattacctga
     8161 attttcatcc cttgagaatg tgatgatacc gggaatgata gctggcactt ctcgttcaga
     8221 attagaaatt agagcgaaag aacttttaga tcaattagga ttgaaagatc gaatggattc
     8281 tannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnattagagc gaaagaactt
     8401 ttagatcaat taggattgaa agatcgaatg gattctaaac catcgacttt atcgggaggc
     8461 gaacaacaac gtgtagccgt agcgcgagca ctgatgaata agcctaaatt gttgttagct
     8521 gatgaaccaa gtggaaacct agattcgaaa aatgcgcgtg aactgcacga gttatttttt
     8581 caattgcgta aagatcttgg acaaaccatt gttttagtaa cccataatga agatctggct
     8641 cgtatggccg atcgtaggtt ggtcatggta gacggccgtt ttcaacaaga aggttgataa
     8701 ttatttcagg aatttcagga tttgcccaaa tattcgtggc acttcaaaaa gctgtcataa
     8761 aaaagactcg agtaatattg tgattcgttc cgggcttcga aacaatcatt acacgatgta
     8821 tgtcaacagc ccaaattcaa gaaacagcag aagcggttct gcaacgttat ttcgggtatt
     8881 ccgaatttag acctcttcaa gaggagatca tttctcaggt tttaagtaat actgattgtt
     8941 tggttttaat gccaacaggc ggagggaaat cggtatgctt tcaagttcca gctttgatga
     9001 aagcaggaac ggcattggtt atttcaccat tactgtcatt gatgaaagac caagttgatg
     9061 cattgaaagc aaatggaatt caagcggcta catggaatag cggcacgtca cctgaggagg
     9121 aatttgacat aattgaattc tgcaaaaacg gaaaactcaa actgctgtat atttctccag
     9181 aacgcttgca ggtactgaag aacagtttta tgtcggaaat acattggtca ctatttgcag
     9241 tggatgaagc gcattgtata tcgagttggg gacatgattt tcgtccggaa tacacgcgat
     9301 taaatggatt gaaagaacta tatccggatg tgccgataat agcactaact gccactgccg
     9361 atttaagcac acgaaaagac attgttgagc aattgaaact tcgcaatgct agacagttta
     9421 tagcatcatt tgatcgtcca aatttatctt tagatgttcg aggagatatg tccaaagaca
     9481 tacaggataa ggaaatactg gctttcattc ggaaacgaag aaatcaatgt ggaatcattt
     9541 actgcagtag tcggaaaaag acggagatgt tatcccattt cctgaatagg caaggcatcc
     9601 gaagtgaaca ttatcatgct ggaatggtgt atccagatcg tgtgcgagta caggagaatt
     9661 ttttgtatga tcgaacgcaa gtgatatgtg ctacgattgc ctttgggatg ggcattgata
     9721 aatcggatgt acgctatgta attcattaca acttacccag aagtgttgaa agttactacc
     9781 aggaaatcgg gcgtgcaggt cgagatggct tgcctgctga aacgctctta tattatcatc
     9841 attctgattt tcgattacaa cgcaagtatg ctttagaaag tggacaagtt gagctgaatt
     9901 tggagaagtt gagattattg aaacattaca ccgaaggaac agcttgtcgc aggaaagctc
     9961 tgttgaatta cttccatgaa gcttatccca gagaatgtgg caattgtgat cgatgtcgag
    10021 ctaggaatac taatcaacac cttgctgaat cagaagttaa acaacctaat ctgcagggtt
    10081 tgctcgaaca tttaaggcaa gtaaggttac aactagctcg tcaagagaaa ttacctgcgt
    10141 acatcatctt tcatgaaagc acgttaaagg agatgtcgga attgagaccg gatcaattgc
    10201 cgggaatatg tcaggtacat ggaatgagtg agaataagtt tcggaaatat ggcaagtact
    10261 ttttcttgga aatagctcag tggtatcgta atcaaggata tagtttacca aatgaaagct
    10321 tgctgcaaac ttggctttta ttgagagaag gtaaatcacc ggaggatgtt gctgtacaac
    10381 gagcactcag tttaagtacc attttccagc atgctggaat cttaatccaa gcagagtatc
    10441 cgttagaaca ggaagctttc ctgtgttccg aaaaacgaaa gcaaattgaa aaagcagtaa
    10501 atgttcttgg ttatactggt aagagaaaac ccatagtgga ttacttcaat ggagcactaa
    10561 gctatcatga gttatccttc tatctcgatt cccaaaaaca gattagtgta tgatagtcat
    10621 ttcatttacc agatgagtcg cacctgcaaa tttgtctatg atgaataatg tataacgaat
    10681 atcagccgtg atgtttcgaa cttgattgat atcgtaatac acatcactca tggttccttc
    10741 ccagttacgg tcgaaattcg ttccgatgag ttgtcctttt gcattaaaaa ccggactacc
    10801 cgagtttcct ccagtggtgt gattggaagc tgcaaaacag gtgtgtaatt gtccgtcttt
    10861 atcggcataa ggaccaaaat ctttcttctg aatgagatct tttaggcgtt ctggaatact
    10921 gtaatcgacc actcccgaat tacttttttg aagcagtcca tcggcagtgg taaaccaagt
    10981 gtagtttacc ccatcggctg gagaatatcc ttcggttttc ccataagcaa cacgaagtga
    11041 ttgattggca tcaggataat agcgtttttc aggaatgacc tcccgttgag cttttacgta
    11101 tagtcgattc atgggaataa ggcgagccat aacaccgttg tagtttttca ataccacatt
    11161 ccgatactga tcaaagtaga aaacggcaag tttgtatgcg atatcacctt tcaattttgc
    11221 gatagctgca gaatctggat tttcgagcag ttttgaaaca gcttgttcat catccaagaa
    11281 gctggagtca aacacaattg ttgttaccac ttctacatca ttagttgtag cttgtgctga
    11341 cagaccagca ataaaatcgg gagagacagg cttaaccgat ttgctgtaat aacttttcag
    11401 catggcagtc atgatttcgc gatcgagtag agaatagtaa tctttgaaga attcggctac
    11461 acgtttcttt tgattagcga gtatttcctt tcctttttca gatttcaaat ctatacttcc
    11521 gtttgtcagc tctcgaaaac cattcgcgaa acgaagaatt tcgatagtga gcaaagcttc
    11581 gttatatagg tctaattcgg gttgaatatc tttcaatgtg gcatactcgc gattaaaatc
    11641 agagagtaga tttttatacg ctgagaattt gggatctgca tctaccctac tctgaaattt
    11701 agattcaaac tctttctttt tatcaatcgc atgagctcgc attaaacctt tagcttcacc
    11761 tgcccatttc ttatggtaat tacttacacc ggaatattta tcggcatact tgataaaaac
    11821 acctcgatcg gccgacatgt atttattcat cacatcgaga cgttgtccgc gaagatcaac
    11881 cttaagagga ttactcaaat tcactatttg atcaaccgcg ttagaagtta aatattcgtt
    11941 ggttcgagct ggaaaaccat acaccatggt aaaatcgttt tccttaattc cactggtaga
    12001 aatagggaaa tggtagcgag gctgaaaagg aacattatcg ggtgaataat cggctggttt
    12061 attatccttt ccggcataaa ttcggaataa actaaaatcg gcagtatggc gcggccaaac
    12121 ccagttgtcg ctgtctttcc cgaagtttcc aattgattgt ggtggagcgc ctaccaaacg
    12181 aacatcccga aatacttctg tcacaaaaag cacatactca tttccattgt acatcggctt
    12241 aatcaaggct tcgtaatgcg tattggcaac agcttctttg actaattcca tcgaacgttg
    12301 agtaatgcga ttctcccgct cactttcgtc cgtgatttta tcgagtccct caatcatttt
    12361 ggctgtgaca tcttccattc gtatgatgaa cgttacggtc aatccaggat tgggtaattc
    12421 atctgcatga ttttttgccc agaaaccatc tttcaaataa tcgtgatcta aacttgagtg
    12481 atcttgaatt tgtccgaaac cgcaatggtg attagttagc aacaaacctt ctttacttat
    12541 aacttctccc gtacaaccgc gtccgaaaag caccacacca tctttcatgc tcgatttgtt
    12601 gacactgtaa atgtcctcag cagagatttt catccccatt ttcttcatgt ccttctcgtt
    12661 gagttgcttc aacagcatag gcagccacat gccttcatcg gcgagaagtc gaacggagaa
    12721 aagagtaaac gacaataaaa gagatagtac acgcatagtg atggaatttc aggccgaaaa
    12781 tggcgaaaaa tattggaatt ctcaggatat gatagctttc gaatagaaga atcgatagtt
    12841 aagaattaca cttagaaatg aatagaatca tacaatgagt tatttcacta aaaaatgacc
    12901 aagtaagtga accccttttt ttgctttgca taaaggcgga actaacccgt ttcttatgtc
    12961 gatccaaatt caagtgcctc agccttgcca tgcttcttgg gataaaatga ctccgcaaga
    13021 aaatggtcgt cattgcaaat cgtgcgataa agttgttatc gattttagtc ggatgaatga
    13081 tgaagaaatc aaaaaatggt ttcgaaaaaa agaaggtgaa aagatatgtg ggcactttcg
    13141 ggttgaccag gtaaaacgcc ttcagatagt tgtaaaaccg agagaattta agcagttagg
    13201 atggtcgccc gtacaactga ttcgggtggc tatttttctt gtttttttct cgagtttatt
    13261 tagttgtagt ccttatcctg agcaaaatgg tgctatgacc gaaattgtca tagatgatga
    13321 aaaagatccc aatgatgaaa taag
//
LOCUS       DAOOQW010000046        13181 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_5797_length_13181_cov_5.471812, whole genome shotgun sequence.
ACCESSION   DAOOQW010000046 DAOOQW010000000
VERSION     DAOOQW010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 13181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13181
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5797_length_13181_cov_5.471812"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            202..573
                     /locus_tag="PK281_04445"
     CDS             202..573
                     /locus_tag="PK281_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48626.1"
                     /translation="MKILSLLLSLYFFAGSLMPGSDWDELPKVVDLIEHYQHHEKESQ
                     GQLSFSDFLFLHYSNTTDHSKDDCKLPFQHAFTSAYTAEIHHPELLIIQLAEYPIQPV
                     LHITKETRDHVSYVFQPPQLG"
     gene            700..5055
                     /locus_tag="PK281_04450"
     CDS             700..5055
                     /locus_tag="PK281_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010250892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HOY48627.1"
                     /translation="MLNKIIEFSVNNKLIIGLFIIALIGFGSYEFTRLPIDAVPDITN
                     NQVQVIAVAPSLGATDIERLVTFPIELANSNIAGMHEIRSFSRFGLSLVTIVFNDDVD
                     VYWARQQVAERLLQVQAEIPKGVGSITLGPVTTGLGEIYQYIVRPKKGYEHKYSAMDL
                     RTIQDWIVRRQLLGVKGVADVSSFGGQLKQYEIAVEPNKLKANNITINDIFAALENNN
                     QNTGGAYIEKGPTVQYIRSEGLVGSIRDIENTFIRNTSNGTPLLIRDVATVQYGSATR
                     YGALCYNDQGEVAGAIVMMLKGANSSEVIHDVKERVAQIQKSLPEGVVIEPFLDRTKM
                     VNNAIGTVETNLMEGALIVLFVLILFLGNLRAGFIVASVIPLAMLFAVIMMNLFGVSG
                     NLMSFGALDFGLIVDGAVIIVEAVLHQLTHSKSFLNINRFSKEQMDKEVKSAAGRMMN
                     SAVFGQVIILVVYLPILSLQGIEGKMFKPMAETVAFALTGAFLLSLTYVPMMTSLMMS
                     KNISHKPTFSDRMMTWVERHYQQALKRVLVIPKSIITGVIVLFSVSLFILSQLGGEFI
                     PALEEGDFAVETRVLTGSNLSTTIESTQKAAGILKSRFPEVLKVVTKIGSGEIPTDPM
                     PMEAADMMVILKDKSEWTSAETFDELAEKMTVAISEVPGITVSFQYPVQMRFNELMTG
                     AKQDVVCKIFGENIDTLAAYAQKLGELSSTVDGTKNVYVEPVTGLPQIVIEYNRAAIA
                     QFGLSIADINKVVNIGLAGQSTGFVYEGEKRFDLVVRLNGQQRKNIEDVRNLLIPTSE
                     GQQVPLSQLANVELKDGPNQIQREDAKRRIIVGFNARGRDVQSIVNELQEKVGKTIKL
                     PPGYFITYGGAFENLNAAKARLSITVPVSLLLIFLLLYFSFNSIRQGLLIYSAIPLSA
                     MGGIFALSLRGMPFSISAGVGFIALFGVAVLNGIVLVAEFNRLKKEGWTDLKKIVVMG
                     TKIRLRPVLMTAFVASLGFLPMAVSNGAGAEVQRPLATVVIGGLLIATFLTLFVLPVL
                     YILFEKKADKKASVKNPPVAQILIAVLLLGAGIGHAQTPIKLQAAIDTALKNNLKVKQ
                     QELLSNYQKQLEKTAWDLPQTSLGVEYGMINSRYTDNRFGLAQSFSFPTVYTRQKQLL
                     REEYKSSLLQLDVNKAELRRSVREAYFNLYYLNEKKKLLQRADSLYGAFLVKAEARFK
                     SGESNILEVTSASAQRGQMNLQLQHLEMEQARVQAEFQLLLNTSTQYIPDVGSTNELT
                     ITPSLQFNSNNPHLLVLEQNKVVSHAAYRTEKARLMPSLNVAYSNMTMFGSGSDNKSY
                     TYNTRFQSGQFGLGLPIFTGAQRARIKAAGIQQQIAEYSFSSGMIKMQSEYQLALQQL
                     DNYRKAQTYYDETGVPNSKLIIETANRQLNNGDINYLEWVILTNQAIQIQNESLGANY
                     QFYNALIHILYLNNN"
     gene            5058..6185
                     /locus_tag="PK281_04455"
     CDS             5058..6185
                     /locus_tag="PK281_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019977009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOY48628.1"
                     /translation="MNKLNILAFLVLFASCQSTNKENPEDSVQVESQTITLNDIQVKN
                     AGIAIGKLEKKTLSRLLNVSGKIDVPPQNMVSVSVPLGGYLKSTQLLPGMHIRKGEVI
                     AVMEDQQYIQLQQDFLVTQAKLNYTEKEFERQKELNQSKATSDKVFQQAESEYKTQKI
                     NLKSLGEKLKLIGIQPEKLSENTISKSINLYSPIDGYVTHVNVNIGKYTQPSEVLFEL
                     VNPADIHLALTIFEKDLDDIFIGQKLIAYNNRNPEKKHVCEIILVGKNLTADRNAEVH
                     CHFEKYDETLIPGMFMNAEINLKSIDTDVLPDDAIVSFEGHQYVFAQDKTNQFTLTEV
                     ETGSSESGFTQILNASQISGKSIVVKGAYTLLMQMKNTADE"
     gene            complement(6479..7906)
                     /locus_tag="PK281_04460"
     CDS             complement(6479..7906)
                     /locus_tag="PK281_04460"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY48629.1"
                     /translation="MKLSTIVSFPLFAAALLVAGNAEAQVNATSVSSFYQGMRADGVS
                     PIGVTRSNPNNALGAINVNDVNIGEVANNSSTVEFVSLGFGGELVLEFASPICNIWGS
                     NDLTVYETSYGSPSCASWPEKALVYARQDECQAWVLISPADGICQNADLDLGVLSWAK
                     YIKIEDITNPTTFLANNQDGYDVDGVVGFATCDGGSYTAGGKYSPNGVASLAQGLRKN
                     GTPVPANRSIQSRMLGAPQMSDASTTPANYPFFALGYGGSVILSFPYTIINVAGADLQ
                     VYETTFGDNPSRNCASYPEKASFEGSIDGSTWFSLDAVVTADDAGSILCRDGKLEIPA
                     AYAGLNFLRVTDVTSPFGPGTTDAYDIDGIVGLSQCGQSTSNPGGQKVATEEEIGEGE
                     FGFDIFPNPASDVASLNVTGVNNMDNYTIKVIDMMGRVISTESVNNGTGSFTHNLGIN
                     KLTAGVYFVSVESNGSREVAKLIKK"
     gene            complement(8263..8823)
                     /locus_tag="PK281_04465"
     CDS             complement(8263..8823)
                     /locus_tag="PK281_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48630.1"
                     /translation="MKQLFTLFLLLSSFIGFSQANKGMEYYDGWACTKKGDTLRGKIC
                     YESTKSGEKFDKIYFMDAAGAKKRLGPEKLVSYGVQGKVFEYILLEEAPSALPMQQVI
                     VGDISLYYGWFPTAETTPQKFFYEKGIFLKRKDQDGYFEVLEKKFQKSMSTYFKGDDD
                     IVKLIKDNNYEAKDIEKIVVAYNAKK"
     gene            complement(8895..9668)
                     /locus_tag="PK281_04470"
     CDS             complement(8895..9668)
                     /locus_tag="PK281_04470"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HOY48631.1"
                     /translation="MKYHLGIKSLILFFFLQVGVISTLASRELPSKPNPPRLVNDFAS
                     ILTESERNQLENKLVKFDDSTSNQIAIVTMSDLDGEPASSYAPELFNSWKIGHDGRNN
                     GILILVSMDNPREVFINTGYGMEEFVPDITAGRIVQEDLIPYFKNKEYYNGLDKASTD
                     LMQLLSGTFKGFPKKQRSRGIPPLVIVILIIVAVILISRKNRGGGRYMRTFGGPGFGG
                     WGGGFGGFGGGSSGGGFGGGGFGGFGGGSTGGGGAGGSW"
     gene            complement(9646..10080)
                     /locus_tag="PK281_04475"
     CDS             complement(9646..10080)
                     /locus_tag="PK281_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019037884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HOY48632.1"
                     /translation="MANKFISKEEESAIISAISLAEQETSGEIRVHLESTCNKDSVDR
                     AIEVFNLLKMDQTLLKNGVLIYVAIKDRKLAIIGDTGINEVVPSDFWDSTRDLLVTHF
                     KDGKYVEGLVHAIEEAGKVLKQYFPFKSDDQNELSNEISLGN"
     gene            complement(10070..10666)
                     /locus_tag="PK281_04480"
     CDS             complement(10070..10666)
                     /locus_tag="PK281_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019388265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOY48633.1"
                     /translation="MKSIVSIGIVLLVVILLFGIGGCSYNKMVQKQEAVSSAWSQVEN
                     VYQRRADLIPNLVNTVKGAANFEKSTLEAVMQARASATSVKIDANNLDAASFQKFEQA
                     QQGVSSALSRLLVSVEAYPELKANQNFRDLQTQLEGTENRIAVERKNFNEVVKDYNSH
                     IRSFPNNLFAGMFGFTVKDYFKAAEGSDKTPEVNFDGK"
     gene            complement(10814..11098)
                     /locus_tag="PK281_04485"
     CDS             complement(10814..11098)
                     /locus_tag="PK281_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48634.1"
                     /translation="MRNVPLAEKETVKNMVFPREEVLKSKDSAIERRTLLERATILSN
                     LYHNKVKIVFEDRDGSKMVDTTIWATTEAAIMLKGGVVIPIHRIHSIDLV"
     gene            complement(11169..12473)
                     /gene="tilS"
                     /locus_tag="PK281_04490"
     CDS             complement(11169..12473)
                     /gene="tilS"
                     /locus_tag="PK281_04490"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOY48635.1"
                     /translation="MKAPENISIDSLQLGGLSKNTPFILAISGGVDSCVLLHILAQAG
                     YRGTLAHVNFGLRGEDSNKDEQLVRQLASAYQWDIEVYDASEEMKTISGESIQMAARR
                     IRYNWFTQLAQKIQAKNVLLAHHADDQVETFFIRLLRGSGSEGLSAIKTGDGFYLHPL
                     LTISKAEIIEYATSNQLIWRDDQSNYSSTYLRNAIRLNGLPVWDEIQPSFRQMVQESV
                     MRLNEENLQLKQFVSNWLINHVLVDSTGKSILKKDLLSQASPVFILHEFLKEYGFSWR
                     ICTDIAQNLTQTHVQYYYAAGKTLRLDRLKVSVYEGVARVSRVENEVCPTISCQHVDE
                     MPDNLSVSDKNTAWLSAPKLKGNLSLRKWRKADVFWPWGMEGRKLLSDYFTDLKLSPE
                     ERNSQWLLMCDDEIVWVVNRRIDRRFAAVSGEKEIIKVQIID"
     gene            complement(12470..>13181)
                     /locus_tag="PK281_04495"
     CDS             complement(12470..>13181)
                     /locus_tag="PK281_04495"
                     /inference="COORDINATES: protein motif:HMM:NF012640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HOY48636.1"
                     /translation="FMPDLEPVQLFKKLCLLSPAPFSCLMKNGDNWLIGSSPERFLKK
                     EDTKLISQPIKGTVRRSANEDIDQQAIAKLKSDPKERAENVMIVDLVRNDLSHFAKRG
                     SVQVAELCEVYPFATVHHMISTVQAELTSADLGLEAIKKAFPMGSMTGAPKIRAMELA
                     DETENFSRGIYSGALGYFTSDGDFDFAVMIRSFTWNAASGYLSFSTGSAITNQAIASA
                     EYHECLLKAEALLKSLQQ"
BASE COUNT         4139 a         2709 c         2422 g         3911 t
ORIGIN      
        1 cattggtatg cgattgaagg ctgatggtat ctccaggtga tgcgcttaaa ttgatacaga
       61 caaaaccgag aagtaaaaat agaatgtgct ttttcatgaa taaaaccatt tacaagaata
      121 caaaatacag ccatttgttt tcgccaatta tcaaatactt cagggtgtta taacaatcaa
      181 atcattgctt atttttgtgc tgtgaaaatt ctgtccctgt tattatcact ctattttttt
      241 gccggtagtc ttatgccggg atcagattgg gacgaattac ccaaagtggt tgatttgatc
      301 gaacattatc agcaccacga gaaagaatca caaggtcaat tatccttttc agattttcta
      361 tttcttcatt attccaatac tacagatcat tctaaagatg attgtaagct gccctttcaa
      421 catgctttca catcagctta tacggccgaa attcatcatc cagaattatt aattattcaa
      481 ttagccgaat accctattca acctgtatta cacattacaa aagaaacgcg agatcacgtc
      541 tcgtatgtgt ttcaacctcc tcaattaggc tgagttgttg acgaattgtc gtcaccccta
      601 tcattttaaa ttgcttcctt attgatgttt ctacacatgc tattgcagca tacttaggaa
      661 gcaaatttcc ctcattgatt cactaattat cgactgataa tgctcaataa aatcatagaa
      721 ttttctgtga ataataagct aatcatcggg cttttcatca tagccttgat tggttttggc
      781 agttacgaat ttacacgact gcccattgat gccgttcctg atatcaccaa taatcaagtt
      841 caggtaatcg ctgttgctcc ttcgcttggc gcaactgata ttgaacgcct cgtgacattc
      901 cccatcgaat tggcaaacag caatattgct ggaatgcacg aaatacgcag cttctcccgc
      961 tttggtcttt cacttgtgac aatcgtattt aacgatgatg tggatgtcta ttgggcgaga
     1021 caacaagttg ctgaacgttt acttcaagta caagctgaaa ttcctaaagg tgtaggctct
     1081 atcacactcg gtccggttac cactggactt ggtgaaatat accaatacat cgttcgtccc
     1141 aaaaaaggct acgaacacaa gtacagtgca atggacttga gaactattca agactggatt
     1201 gttcggcgac aacttttagg agttaaagga gtggcggatg taagtagttt tggtggacaa
     1261 ctcaagcaat atgaaatagc tgttgagccc aataaactca aggctaacaa cattaccatt
     1321 aacgatattt ttgccgcgct agaaaacaat aatcaaaaca cgggtggtgc atatatcgaa
     1381 aaaggtccaa cggttcaata tatccgaagc gaaggtttag ttggtagcat tcgggatatc
     1441 gaaaacacat tcattcgaaa cacaagcaat ggaacgcctt tgctaattcg cgatgtagct
     1501 actgttcaat atggaagtgc tactcgttat ggtgctttgt gttataacga ccaaggcgaa
     1561 gtggcgggag cgattgtcat gatgctcaaa ggcgccaata gcagcgaagt aattcacgat
     1621 gtgaaagaac gcgttgctca aattcaaaag tcattgccag aaggtgtggt tatcgaaccc
     1681 tttctcgatc gaactaaaat ggttaacaat gccatcggaa cggtcgaaac aaacctcatg
     1741 gaaggtgcgc taatcgtact attcgtgctc attcttttcc ttggaaatct acgagcagga
     1801 tttatcgttg cctcagtcat tccgctagcc atgctgtttg ccgtaatcat gatgaatctt
     1861 tttggggtaa gtggaaacct catgagtttt ggagcgctcg atttcgggtt gattgtcgat
     1921 ggagctgtaa tcattgttga agcggtacta catcaactta ctcacagtaa atccttttta
     1981 aatatcaatc gattctcgaa agagcagatg gataaagaag ttaaaagcgc tgctggaaga
     2041 atgatgaata gtgctgtttt cggtcaagta atcatcctcg tcgtttacct gcccatcctt
     2101 agtttacaag gaattgaagg taaaatgttt aagccaatgg ccgaaacggt agcatttgcc
     2161 cttacaggtg cttttctgct ttctctcact tatgttccta tgatgacatc tttgatgatg
     2221 agtaaaaaca taagtcataa accaactttt tcagatcgga tgatgacttg ggttgaaagg
     2281 cactatcagc aagcattaaa gcgggttttg gttattccaa aatctataat tacaggtgtt
     2341 atcgtattgt tttctgtttc cctttttatt ctttcccaat taggtggaga atttattcct
     2401 gcacttgaag agggtgattt tgctgttgaa actcgagttc taacaggtag taatttatct
     2461 actacaatcg agagcactca aaaagctgca ggtattctca aatctcgttt tccagaagtg
     2521 ctaaaagtgg tgaccaaaat tggaagcggc gaaatcccga ccgatccaat gcctatggaa
     2581 gcagctgata tgatggtgat tctcaaagat aaaagtgaat ggacatctgc cgaaactttc
     2641 gacgagttag ctgagaagat gactgttgcc atttctgagg tacctggaat tacagtgagt
     2701 tttcaatacc cagttcaaat gcggtttaac gaattaatga cgggtgcgaa gcaagatgtg
     2761 gtttgtaaaa tttttggtga aaacatcgac acgctagctg catacgctca gaaactaggt
     2821 gagctttcat ctactgttga tggaaccaaa aatgtgtatg ttgaacccgt tactggctta
     2881 cctcaaattg taatcgaata caatcgtgct gctattgctc agttcggttt gtcaattgcc
     2941 gatatcaata aagtcgtaaa cattggacta gcaggccaaa gcaccggatt tgtctacgaa
     3001 ggcgaaaagc gtttcgattt ggttgtacgc ttaaatggac aacaaaggaa aaacatcgaa
     3061 gatgtgcgaa acttgctcat tcctaccagc gaaggacaac aggttccact ctcccaatta
     3121 gccaatgtag aattgaaaga tggtcccaat caaattcaac gtgaagatgc taaacgtcgg
     3181 attattgttg gatttaatgc acgtggcaga gatgttcagt cgatagtaaa tgaacttcaa
     3241 gaaaaggttg gaaaaacaat caaacttcct cctggatatt ttatcacgta tggtggagcc
     3301 ttcgaaaacc tcaatgctgc aaaagctcga ttgagtatta cagttccagt atccttgctc
     3361 ttaatctttc tcttgcttta tttttcattc aattcaatcc gacaaggttt gctaatctat
     3421 tcagccattc cgctttctgc aatgggagga attttcgccc ttagtttaag aggaatgcct
     3481 tttagtatct cagcaggagt aggtttcatc gcactttttg gagttgctgt attaaatggg
     3541 atagtactcg ttgcggagtt taatcgctta aagaaagaag gctggaccga cctcaagaaa
     3601 attgtggtta tgggaactaa aattcgtctt cgtccagtgt taatgactgc ttttgttgca
     3661 tcacttggat tccttccgat ggctgtcagt aatggtgccg gagcagaagt tcaacgacca
     3721 cttgctaccg ttgttattgg aggtttattg attgctacat tcttaacact attcgttctg
     3781 cctgtcctgt acattttatt tgagaagaaa gcagataaaa aagcctcggt taaaaatcct
     3841 ccagtagcac aaattctaat tgctgtattg cttttaggag caggaatcgg tcatgctcaa
     3901 actcctataa aattacaggc agcaattgat acagctttga aaaataatct gaaggttaag
     3961 caacaggaat tattgagcaa ctatcaaaaa cagctcgaaa aaacagcttg ggatttacct
     4021 caaactagct tgggtgttga atatgggatg attaatagcc ggtatacaga taatcgattc
     4081 ggcttagctc aaagctttag ttttcctact gtgtataccc gacagaaaca actgcttcgt
     4141 gaagaataca aaagcagttt gctccaatta gacgtcaaca aagcagaact tcgtcgttcg
     4201 gttcgagaag catatttcaa cttgtattac ctaaacgaga aaaagaagct tttacaaaga
     4261 gccgacagtt tgtatggtgc atttctagta aaagcagaag cccgtttcaa aagtggagag
     4321 agtaacattt tagaggtaac atctgcatcc gcccaacgcg gacaaatgaa cttacaatta
     4381 cagcatctcg aaatggaaca agctcgtgtg caagctgagt ttcaactttt attaaatacg
     4441 agtactcaat atattccgga tgtaggttct acgaatgaat tgactattac tccttcgtta
     4501 cagttcaatt caaacaaccc gcatcttttg gtattggaac agaataaggt tgttagtcat
     4561 gcagcttatc ggacagagaa agcgcgtcta atgccttcat taaatgtggc ttatagtaat
     4621 atgaccatgt ttggttctgg gtcagataat aaaagctaca catacaacac acgttttcaa
     4681 tctggacaat tcggtctcgg acttccaatt tttactggag cacaaagggc acgaataaaa
     4741 gcggcaggta ttcaacagca aattgccgaa tattcatttt caagtggaat gataaagatg
     4801 cagtcggaat accaacttgc tttacaacag ctcgacaatt atcgtaaagc ccagacttac
     4861 tatgatgaaa cgggagttcc aaactcaaaa ctcatcatag aaaccgcaaa tcgccagctg
     4921 aataatggtg atatcaatta cctcgaatgg gtgatcttga ccaatcaagc aatccaaatt
     4981 caaaatgaat cattgggtgc taattatcaa ttttataacg ccttgattca catcctttat
     5041 ctcaacaaca actgattatg aataaactca atatactggc atttctcgtt ttattcgcgt
     5101 cttgtcaatc aacaaacaaa gagaatcctg aggatagtgt acaggtagaa agtcaaacca
     5161 ttaccttaaa tgatattcag gttaaaaatg ccggaatcgc aatagggaaa ctggaaaaga
     5221 aaacactttc acgcttatta aatgttagcg gaaaaattga tgtccctcct caaaatatgg
     5281 tttctgtaag tgttccattg ggtggctatt tgaagtcgac tcaattgctt cccggcatgc
     5341 atatccgcaa aggagaagtc attgctgtaa tggaagatca acaatacatt caattacaac
     5401 aggactttct tgtgactcaa gcaaaactga attacacaga aaaagaattt gaacgacaaa
     5461 aagagttgaa tcaaagcaaa gccacgagtg acaaggtttt ccaacaagct gaatcggaat
     5521 ataaaacaca gaaaatcaac cttaaatcac tcggagaaaa actgaaatta attggaattc
     5581 aaccggagaa attatccgaa aacaccattt ccaaaagcat taatttgtat tcgcctattg
     5641 atggatatgt aacgcatgtg aacgttaata ttggcaaata cacacaacct agcgaagtgc
     5701 tttttgaatt ggtcaaccca gccgatatcc atttggcatt aaccattttc gaaaaagatt
     5761 tagatgatat tttcatagga cagaaactaa tagcatacaa caatcgaaat ccggagaaaa
     5821 aacacgtatg tgaaattatc cttgtaggta agaatctaac ggcagatcga aatgcggaag
     5881 ttcattgtca cttcgagaaa tacgatgaaa cattgattcc gggaatgttt atgaatgcgg
     5941 agattaattt aaaaagcatc gatacagatg tgttgccaga tgatgcaatc gtaagcttcg
     6001 aaggtcatca atacgttttt gcacaagata aaacgaacca attcacacta acagaagttg
     6061 aaacgggaag tagcgaatca ggatttactc aaattctaaa tgcatcacag atttcgggta
     6121 aatcgatagt tgtgaaagga gcttacacgc ttcttatgca aatgaaaaac acagctgacg
     6181 aataagattc attaatctaa atacagttaa accgataagt tctaaacgga actgtcggtt
     6241 tttttatgcc ctgctgctca atagaattgt tctttcggtg agagtcttca attctcgtta
     6301 acatatttct aattgagtat ctactaccaa taaatcaata tttattatag tatcacaaag
     6361 cttagtataa aatagagtaa gagtgtttga tacaaataag accgtatact aattgctaaa
     6421 aattaggcaa aaaaaaaccc tgaccgattg gtcagggttt tttagaaaag cagattgatt
     6481 attttttaat caatttagct acttcacgtg aaccgttaga ttcaacagat acgaagtaaa
     6541 cacctgcagt aagtttgttg ataccaaggt tgtgtgtgaa actaccagtt ccgttgttta
     6601 cagactcagt agagataaca cggcccatca tatcgataac tttgattgtg tagttatcca
     6661 tgttgttaac accagtaaca tttaaagaag caacatcaga agctgggtta gggaagatat
     6721 cgaaaccgaa ttcgccttca ccaatttctt cttcagtagc tactttttga ccacctgggt
     6781 tagaagttga ttgaccacat tggctaagac ctacgatacc atcgatatca taagcatcag
     6841 ttgttccagg accaaaagga gaagttacat cagtaacacg taggaagttc aaacctgcgt
     6901 aagcagcagg gatttctaat ttaccatcgc ggcaaaggat agaacctgca tcatcagcag
     6961 taacaactgc atctaggcta aaccaagttg aaccatcgat agaaccttcg aaagaagctt
     7021 tctctgggta actagcacag ttacgtgatg gattgtcacc gaaagttgtt tcgtaaactt
     7081 gaagatcagc tccagcaaca ttgatgatag tgtaagggaa agaaagaatt acacttccac
     7141 catacccaag agcgaagaat ggatagttag caggagttgt agaagcatca ctcatttgag
     7201 gagcacctaa cattctagat tggattgaac ggttagctgg tactggagtt ccgtttttac
     7261 gaagtccttg tgctaaagaa gcaacaccat ttggtgaata cttaccacca gcagtatatg
     7321 aaccaccatc acaagtagca aaacctacaa caccatcaac atcataacca tcttggttat
     7381 ttgcaaggaa agttgttgga ttagtgatgt cttcgatttt aatgtattta gcccagctaa
     7441 gtacacctaa atcaagatca gcattttggc agataccatc agcagggcta atcaaaaccc
     7501 aagcttgaca ctcatcctga cgtgcataaa ccaaagcttt ttctggccat gaagcacatg
     7561 aaggtgaacc gtaagaagtt tcataaacgg ttaaatcgtt gcttccccag atgttgcaaa
     7621 ttggacttgc gaactcaaga acaagctcac caccaaatcc aagagatacg aattcaactg
     7681 tagaagagtt attagctact tctccgatgt tcacatcatt tacgttgatt gctccaagag
     7741 cattgttagg gttgctgcga gtaactccaa ttggagaaac accatcagca cgcattcctt
     7801 gatagaagct agaaacgcta gtagcattta cttgtgcttc tgcatttcct gctacgagca
     7861 aagctgctgc gaaaagcgga aagctaacaa ttgtagataa tttcatactg ttttttgttt
     7921 tagttaggtt aacagggtca aagttatcta ttcttacaga gaagtcaaat taaagttaca
     7981 aaaaagtaag agtaattatt tttcacacaa tcgccttctg cgataatcga acgcggtttt
     8041 ggctattttt tgtacctaat ggacattttg gcaaaagttg tcaaccaata tgaacaataa
     8101 aacttacaca tgatgtgtcg cgataggggt ataaactcac actacccatg caattagtat
     8161 tgtctttcta ccaaacacat taagtctcca gtttttcgca tgggtagaaa aagaaaaagc
     8221 aggaaataaa tcctgcttca acttaatatc gatgtattgc tattactttt tagcattgta
     8281 tgcgacaaca attttctcaa tgtccttagc ttcgtagtta ttgtccttta taagcttcac
     8341 aatatcatcg tcgcctttga aataagttga cattgatttt tgaaacttct tttccaatac
     8401 ttcaaaatat ccatcctgat cttttcgttt caaaaagata cccttttcat aaaaaaactt
     8461 ttgtggtgtt gtttcagcag ttggaaacca tccgtaatac aaagatatat cgccaacaat
     8521 tacttgttgc atgggtaatg ctgaaggggc ttcttccaat aagatatact caaatacttt
     8581 cccctgaact ccatatgaaa ctagtttctc tggacctaag cgctttttag caccagcagc
     8641 gtccatgaaa taaatcttgt cgaacttttc tccagatttt gtgctttcgt aacagatctt
     8701 gcctcttaga gtatcacctt ttttagtaca agcccatcca tcgtaatatt ccatgccttt
     8761 gttggcctga ctaaatccaa taaatgaaga taaaagtaaa aacagggtaa aaagttgttt
     8821 catagtaaga gatgaattgt tgtttccaaa cctaaaagat tttctgtcag gaaacaatgc
     8881 gtgttaaaaa taagttacca gctaccacct gcaccaccgc cgccagtact tcctccgcca
     8941 aatcctccga agccaccgcc gccaaatcct cccccagaac ttccgcctcc aaaacctccg
     9001 aagccgccgc cccaacctcc aaatccaggt ccgccaaagg ttcgcatata tcttcctccg
     9061 ccacgattct tgcgcgaaat aagaattaca gctacaatga ttagtattac aattactaat
     9121 ggtggaattc ctcgtgatct ttgtttctta ggaaagccct taaaagtacc tgacaataat
     9181 tgcattaaat cagtactcgc cttatccagt ccattataat attccttgtt cttgaaataa
     9241 gggattaaat cctcttgaac gattcggcca gcagttatgt cgggaacaaa ctcttccatt
     9301 ccataacctg tattaataaa tacttccctt ggattatcca tggaaaccaa tatcaatatt
     9361 ccattattgc gaccgtcatg tccaattttc caagaattaa ataattctgg ggcatacgaa
     9421 gaagctggct ctccatctaa atcagacatc gttacaatgg caatttgatt ggatgtgcta
     9481 tcatcaaact taaccagctt attctccaat tgatttcgct ctgattccgt taaaatggat
     9541 gcgaagtcgt ttactaagcg aggcggatta ggcttgctag gtaactctct acttgctaga
     9601 gttgaaataa ctcctacttg tagaaaaaag aagagaataa gggatttaat tcccaagtga
     9661 tatttcattg gataattcgt tctgatcatc acttttaaaa gggaaatact gctttagtac
     9721 tttacctgct tcttcaattg catgaactaa accttctaca tacttgccat ctttaaaatg
     9781 cgtaaccagt aaatctcgtg ttgaatccca aaaatcactt ggaacaactt cgttaattcc
     9841 tgtatcacct atgatagcca gtttgcggtc cttaattgcc acataaatca aaacaccatt
     9901 tttcaatagc gtttgatcca ttttcaaaag attaaatact tcaattgctc gatcaactga
     9961 atctttatta caagtagatt cgagatgcac tcgaatttct cctgaagttt cttgttcagc
    10021 cagagaaatt gcgcttataa tagcagattc ctcttcttta gagatgaatt tatttgccat
    10081 caaaattcac ttctggagtt ttgtcagaac cttcggcagc tttgaaataa tccttcaccg
    10141 taaaaccaaa cattccagca aataagttat ttgggaatga acgaatatgg ctgttataat
    10201 ctttaaccac ttcgttaaaa ttcttccgct caacagcaat ccggttttcg gtgccttcca
    10261 attgcgtttg taaatcgcgg aagttttgat ttgcctttag ttctggataa gcttctactg
    10321 aaaccaataa acgagaaagt gcagaactaa caccttgttg agcttgctcg aatttttgaa
    10381 aacttgcagc atctaagtta ttcgcatcaa tctttacgct tgtcgcacta gcacgcgctt
    10441 gcatgacagc ctctaatgtg cttttctcaa aattagcagc cccttttacg gtattaacca
    10501 aattgggaat caaatcagct cgacgctggt aaacattttc aacttgcgac caagcgctag
    10561 aaacagcttc ctgtttctga accattttat tgtaagaaca tcctccaatt ccaaaaagga
    10621 gaattacaac taaaagaaca ataccaatgg aaacaatact tttcatgtat gaatgggttt
    10681 aaaaacaaga gccgggaaac aacaactgtt ccccggctca aattacaata aacctctata
    10741 caacatgcgc cactgagact cttttcatca atttccaacc acaaaaactg acgaagtctg
    10801 tcaaattggc ttattaaact aaatctatgc tgtggatacg gtgaatagga ataaccactc
    10861 ctcccttaag cataatagct gcttcggttg tagcccaaat agttgtatca accattttac
    10921 taccatcccg gtcttcaaaa acaatcttca ctttattgtg atatagattg ctcaatatcg
    10981 tagctcgttc gagcaatgtt ctacgttcaa tcgcagaatc tttacttttt aaaacttctt
    11041 cccggggaaa aaccatgttc ttcaccgttt ctttttcagc caggggcaca ttacgcatat
    11101 atcaggtttt gaatatttgt ttctccctcc tattacgtcc ttggtaaaaa gtcggtttcg
    11161 tttactccct aatcgatgat ttgtacttta ataatttctt tctctcccga aactgcagcg
    11221 aaccgcctgt caattctacg atttacaacc caaacgatct catcatcaca catcaataac
    11281 cattgtgaat tacgctcttc gggtgaaagt tttaaatcag taaaataatc acttaataat
    11341 ttacgtcctt ccatgcccca aggccagaat acatcagcct ttcgccactt tcttaaactc
    11401 aaatttccct tcaatttggg agcagacaac cacgctgtat ttttgtcgct aactgacaga
    11461 ttatcaggca tttcatctac atgttggcaa gatatagtag gacacacttc gttttccacc
    11521 cgtgaaactc gtgcaacacc ctcataaaca gacactttaa gcctgtctag acggagggtt
    11581 ttacccgctg cgtaatagta ctgaacatgg gtttgagtaa ggttctgggc tatatctgta
    11641 caaatccgcc aggaaaatcc atattccttt aaaaattcat gcaaaataaa aacgggtgaa
    11701 gcttggctca acaaatcctt tttcaatatg gatttcccag tagaatcaac tagtacatga
    11761 ttaattagcc aattagaaac gaactgtttt agttgcagat tctcctcatt caaacgcatg
    11821 acagattctt gaaccatttg cctaaaactc ggctgaatct catcccaaac gggcaagcca
    11881 tttaaacgaa tcgcattcct caaataagtg ctggagtaat tactttgatc gtcacgccaa
    11941 atgagctgat tactagttgc atactcaata atttcggctt tgctgatagt aagtaatgga
    12001 tgcaaataaa atccatctcc tgttttaatg gcagaaagtc cttcggaacc agaacctcgt
    12061 aacagtcgaa tgaagaaagt ctctacttga tcgtcggcat gatgcgccaa tagaacattc
    12121 ttagcttgta ttttttgtgc gagttgagta aaccaattat accgaatgcg cctcgctgcc
    12181 atttgtattg attcacctga aatggtcttc atctcctcac tcgcatcata aacttcaata
    12241 tcccattggt aagctgaagc aagctgacga accaattgtt catctttatt cgaatcttct
    12301 ccacgcagcc cgaagttcac atgggcgaga gttccacgat acccagcttg tgcaagaata
    12361 tgtagcaaca cgcaagaatc tacacctccg cttattgcta aaataaaggg cgtattctta
    12421 gataaacctc ctaattgcag cgaatcaatc gagatgtttt cgggagcttt cattgttgca
    12481 aacttttaag cagagcttcc gcttttaata ggcattcatg gtattctgca cttgcaatag
    12541 cctgattagt aattgcacta cctgtactaa atgataagta tccactagcg gcattccatg
    12601 taaatgagcg aatcatcact gcaaaatcaa aatctccatc tgaggtaaaa taaccaagtg
    12661 cgccagaata aattcctcga ctaaaattct cggtttcatc tgccaattcc attgctctga
    12721 ttttaggagc gccagtcatc gaccccattg gaaaagcctt tttaattgcc tccaatccca
    12781 aatcagcaga agttaattca gcctgaacag tagaaatcat gtgatgcaca gttgcaaatg
    12841 gatatacttc gcaaagttcg gctacttgta cacttcctcg ttttgcgaag tgtgataaat
    12901 catttcgaac caaatcaaca atcatcacat tttcagcacg ttcttttgga tcagatttaa
    12961 gttttgcaat agcttgctga tcaatatctt catttgcact tcttcttact gtacctttta
    13021 tgggttgcga aataagtttg gtgtcttcct tttttaaaaa ccgttctgga cttgatccaa
    13081 ttaaccaatt atcaccgttt ttcatcaaac aagaaaaagg agcaggagat aacaaacaaa
    13141 gctttttgaa tagttgcaca ggttctagat caggcataaa a
//
LOCUS       DAOOQW010000047        13081 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_5868_length_13081_cov_4.242361, whole genome shotgun sequence.
ACCESSION   DAOOQW010000047 DAOOQW010000000
VERSION     DAOOQW010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 13081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13081
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5868_length_13081_cov_4.242361"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="PK281_04500"
     CDS             <1..510
                     /locus_tag="PK281_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48637.1"
                     /translation="WDGIKADPVNYLSISLQRGLWTLPTEKQESICIYNNAQGYLDFI
                     QGYQSSHHQPLLDKINEQSIGKSSYSKRMIWLYSYWNTAFLIMNNKLFYMLFYGIYML
                     LIGLEIRRIYLFRKIDPIKILLMIVPLAMSLAACSLEVIHNRYYLPGILLQLIFLTIG
                     LRRKKLITN"
     gene            669..1616
                     /locus_tag="PK281_04505"
     CDS             669..1616
                     /locus_tag="PK281_04505"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HOY48638.1"
                     /translation="MRKPFILLGLLLLISPLIKAQDPAFTQYNGTAVYTNPALTGSSG
                     ATRIQGAQRIQWPSLPSAYYTTNLAADFSFAKLPVDFGTFYSYDNAGNGSLISSNLGI
                     SFARSHKIYKSLSVRWGVSASIASRKLDPSLLTFGDQIDSRYGFIYSSNTTISYPNVH
                     YALLNAGLVIHAKRFLIGYSAANVNQPNFGFMAESRLPIRQTLHSSLCLFQKEGKRKS
                     ALYANVLYMKQQGFHQILPSISYRYGKWKAGCSFRKGDAAIGMLGYTGKRLSIAYSYD
                     YTISTLTNQTGGAHEFTLGLILGKLNEKRPSMRWTEDLF"
     gene            complement(1824..3116)
                     /locus_tag="PK281_04510"
     CDS             complement(1824..3116)
                     /locus_tag="PK281_04510"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY48639.1"
                     /translation="MLKCILFITLFLFSLVLGAQNLVRNPSFEETDTCYTNCESTTEE
                     QNLNAIGWFNPTLSSPDVYSSCNQPISLPQTEPEQYACLNQFSPFNWRGFQIPHQGSN
                     YIGIAVELVNIQTYNIIPGWREYIASEFISPLENKKYCVSMNLSQASTLYYKYLLNQD
                     NALYSVVNKMGFNITLERPNYITNGSVYLDPSITFTPENDSYFSDTLNWEKVVMPYKA
                     NGGERYFTIGNFYNENDIQFEVITNFDLSNPETAYTNADCYLYLDDVEVIEIPALVSS
                     SDTSIYYGSTIQLSTSTPTEGYEWFEGDTLHSLGGGNSISVNPELTTSYYLKANQCKL
                     ITWDTVNVTVIPKPIIPVSVSFLNTITSSQFQIHYTGDFKPVLNVELFNSVGQLIQQF
                     QISESTPISLENVSAGIYYCRLSKDGVPVMTGKVVKVN"
     gene            complement(3470..7159)
                     /gene="metH"
                     /locus_tag="PK281_04515"
     CDS             complement(3470..7159)
                     /gene="metH"
                     /locus_tag="PK281_04515"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF007024.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HOY48640.1"
                     /translation="MSLLQEQLKKRILVLDGAMGTMIQRHQLEEEDFRKGHFENHPSP
                     LKGNNDLLALTRPEIILGIHEAYYEAGADIAETNTFSGTSIAQADYRLEHIVYDLNVA
                     AARLARQAADKYTALQPDKPRFVAGSIGPTNRTASISPDVNDPGYRAITFDELVEAYT
                     EQVRGLIDGGVDILLVETVFDTLNCKAALFAIDQYCEKQGIRMPVMVSGTITDASGRT
                     LTGQTAEAFLYSVSHIDLLSVGFNCALGARQLKPYIETLAKNSKFRISAHPNAGLPNA
                     FGQYDETPEQMGVQIREYLENNFINIIGGCCGTTPDHIREIARVAAEYKPHVIPDVEP
                     VPFYSGLEPVTIREETNFVNIGERTNVTGSKKFARLIMEGNYDEALTVALNQVEGGAQ
                     ILDVNMDEGMLDSEAAMVKFLNLMASEPDIAKLPFMIDSSKWSVIEAGLKCVQGKSIV
                     NSISLKEGEDAFRHHARLVKRYGAAVVVMAFDEQGQADSYERRIEICQRAYNILVNEV
                     GLPPTDIIFDPNILTVATGIEEHNNYAVDFIKATRWIKNNLPGARVSGGVSNISFSFR
                     GNDRVREAMHSAFLYHAIKAGLDIGIVNAGQLEVYEEIPKDLLELVEDVLLNRRPDST
                     ERLVEFAETIKSKGKVQEKDLEWRTWPVNKRLSHALIKGIVEFIDEDTEEARQLVDRP
                     LQVIEGPLMDGMNIVGDLFGSGKMFLPQVVKSARVMKKSVAYLLPFIEAEKREGESNS
                     NGTILMATVKGDVHDIGKNIVGVVLGCNNYEVVDIGVMVPYQKILDTAREIGADIIGL
                     SGLITPSLDEMVTVAREMEKQGFKIPLLIGGATTSKTHTAVKIAQNYSGPVVHVLDAS
                     RSVPVAGSLINPELRPKFMEDLDVEYQKIREQHAGRVSAKEYISLSAARENRIPIEFK
                     ASDVQTPAHIGNTTLVNYDLAELAQYIDWTPFFATWELYGKYPAILTDSVVGEEATKV
                     FADAHAMLKQLIDEKWLQANAVIGLWPANSVGDDIEIYTDETRTQVLETVHNLRQQNK
                     KAAGQANYCLSDFVAPKSSGIADYIGGFAVTTGLGIEEHVARFEANHDDYSAIMLKAL
                     ADRLAEAFAERLHQRVRQEFWGYASNETLSNEELITEKYRGIRPAPGYPACPDHTEKG
                     LLFQLLDASKQTGISLTESYAMYPTAAVSGWYFGHPESKYFGLGKINRDQVEEYAQRK
                     NMSLEDTERWLSPVLAYEPGLVPVS"
     regulatory      complement(7238..7338)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(7385..7759)
                     /locus_tag="PK281_04520"
     CDS             complement(7385..7759)
                     /locus_tag="PK281_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48641.1"
                     /translation="MYSTKNCPECGEIVHGRSDKRFCSDLCRNVYHNRERISESEAVK
                     EVNLILSRNRKILNTCFQGRVRRVASSYLSARGFDFRYFTHQSINPNGDVTLFCYEIG
                     YQKLDNDKIALQRDMNRVRLLA"
     gene            7985..8332
                     /locus_tag="PK281_04525"
     CDS             7985..8332
                     /locus_tag="PK281_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020080073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOY48642.1"
                     /translation="MNNLKNSVRLIGNLGGNPEVRELPSGKKLAKFSLATTDKFKDSE
                     GKLVNETQWHNLIAWGKTADFIEKYVEKGQEVVIEGKLQSRSYSDKEGAKKYITEISV
                     NEILKIGNGKRKE"
     gene            8499..9851
                     /locus_tag="PK281_04530"
     CDS             8499..9851
                     /locus_tag="PK281_04530"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOY48643.1"
                     /translation="MLSPSIYSSILPSLIILVGIGMLFRSLKLGYEIQRVSEEVSQKR
                     WKLINRLIKGFIVFYGIAICVSWLSFQPWVIFVFALLLLGGAYFVFKILQLSKFSLLT
                     FVKTHHSQAFLMEIFQTMGNALIVADKEGMITLVNPCLTRLIGIPQEEILGRKAEELF
                     LSEISDMPGVQHENGLLLPSLKEIPVLVTISHTYQQGNLIQIIVLQDIRKQKENEIRL
                     KKYLQKIEQNNLELEQFSYVAAHDLKAPLRAIQNLTEFIEEDMKQVPSGVQQHFQLIK
                     SRIARMENLINGLLEYARVGKKGVNLEITNVKELLEDIVDNLGVEKRSRMEIQSELPT
                     LFTHKLLLEQVFANLISNAHKYNTNPSPVIKIGSRDDGKQFEFSVSDNGPGIPADSKE
                     KIFKIFQTLQARDDYESTGVGLSIVKKIVEETGCEIHVDSEPGEGCTFRFTWPRSGAA
                     "
     gene            complement(9848..10300)
                     /locus_tag="PK281_04535"
     CDS             complement(9848..10300)
                     /locus_tag="PK281_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48644.1"
                     /translation="MKNYATLLILFFSLSTAVFAQHSKKGFEYTGTKTRTSLIGKSKM
                     FSEGVKPVEYSIYWNKKKNVIHVNAGEKEKWYSIELAQVDTIHGNVTYIGYIEGTTRN
                     TDSTVPDKNRCEVRFLIDKVSIKIGEARIEDYLLDGAKVWDDRPISTN"
     gene            10425..10940
                     /locus_tag="PK281_04540"
     CDS             10425..10940
                     /locus_tag="PK281_04540"
                     /inference="COORDINATES: protein motif:HMM:NF025227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 family protein"
                     /protein_id="HOY48645.1"
                     /translation="MRVKPEIKYGLLGALLLIIWTLTQYFAGFHTYHFAIAQYTGYGN
                     FLIVFICLWFGLKEKSRDYKGQFSVRRGVKESMYQLFITASLASLFMFLYDYKINSLW
                     VEEMVTWQRQNSSSFSLNLFLANDPHAEAIILSNTETHLCLYFLGIVVVGASMAFMIS
                     AMISKSKPSNA"
     gene            complement(10957..12054)
                     /locus_tag="PK281_04545"
     CDS             complement(10957..12054)
                     /locus_tag="PK281_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48646.1"
                     /translation="MIRRISAVLFLLFVSASAFAQKEVIVLKNSMIRMSDQKFYIKSV
                     IDGRGHTSDIGYVHKGSFNKKEHVELQGGLEKALTDFYTYAVTPDTSLIPIVIRVVFL
                     QVSERTDGPSEIGKAELKVEFYKPNAEGKWGKVFETEAQTEEVAPDVTSGHERRLRKV
                     LESVILSFNNSNWKTVQPEFIEYAEIRGQKSILLNNALVPEKKTWTSLIQAHAAFGSN
                     AEGWGLSYLGVSSVMKGSWAFPISVAMDKYVIDPGLILRNGYETAKLNYGKIGLGAMK
                     KIGGDFHFLFNVNIPLGVEELVRKNQVGSAITYTETTNFIYGVEPTQSFYFMTRSRIG
                     FYLGAGLYERFFKSEVYKSDLGLKIEAGLKF"
     gene            complement(12129..>13081)
                     /locus_tag="PK281_04550"
     CDS             complement(12129..>13081)
                     /locus_tag="PK281_04550"
                     /inference="COORDINATES: protein motif:HMM:NF026121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4476 domain-containing protein"
                     /protein_id="HOY48647.1"
                     /translation="RYTYVDGFVRGECFTTYQLIELLGYISEENDRLSLAMAAYPRLL
                     NKQDVYDIYNSFAYFSSAFRLHDYIATSQKPVTVVPSNPIPPKPLEINFPVLNYPDAA
                     NYNGPKNCVTSLSDNDFMMFVREIATHKSEQEKNEAALSLATRTCLTTSQSMKLATAL
                     NIENNRLELLKKAYLRVYDEANFDFATQVFTHVPNQAALKEFLVVSRKNAQAQLPPPP
                     CIVNDNDFRTIKEPLANESSSSTRLTIARQQIPRFNCYKSRQIKELVALFSSSTDKLI
                     LSKFAYDYVSDKENYFFEISPLLPASSDRQSLSTYIASKR"
BASE COUNT         3960 a         2524 c         2627 g         3970 t
ORIGIN      
        1 tgggatggaa ttaaagctga cccagtgaat tacctttcca tttcgctaca acgcggactg
       61 tggactttac caaccgaaaa acaagaaagc atctgtattt ataacaatgc tcaaggatat
      121 ctcgatttta ttcaaggata tcaatcttcc catcatcaac ctttattgga taaaatcaat
      181 gagcaatcca ttggaaaaag tagttattca aaacgaatga tttggctgta tagctattgg
      241 aatactgctt ttttgataat gaataataaa ctcttttaca tgctttttta tggaatttat
      301 atgctgttga taggtttaga aattcggcgc atttatttat ttcgtaaaat cgatccgatt
      361 aaaattttat tgatgatagt tccgctggca atgtcgctgg cagcatgctc cttggaagta
      421 attcataatc gatattattt accgggaatt ttgttgcaat tgattttttt gacgattgga
      481 ttgaggagga aaaaattaat tacgaattag taattacgaa ttacgaatgg acagtgaatt
      541 acgaatggac aattacgaat tacgaataga taatttcaaa tttattcaag gaaacatttt
      601 ggaacaatca taaactcatg attaattcgt taattgagtt gaatttttga aatcagattt
      661 atagtaggat gagaaaacca tttattttac tgggattatt actactcatt tcaccattaa
      721 taaaagctca agatccagct tttacacagt ataatggaac ggcagtatat acgaatcctg
      781 cattaactgg aagtagcggc gcgacacgta ttcaaggggc gcagcgcatc caatggcctt
      841 ctcttccgag tgcttattat acgactaacc tagcggctga tttcagcttt gcgaaattac
      901 ctgtcgattt tggaacgttt tattcgtatg ataatgcagg aaatggatcg ctcatttcaa
      961 gcaacttagg tatcagtttc gcacgatctc ataagattta taaaagctta agtgtccgtt
     1021 ggggagtatc tgcttcaatt gcttcaagaa agttagatcc atctttactc acttttggag
     1081 atcaaatcga ttcgcgttat ggttttattt attcatcaaa cacaaccatc tcctatccaa
     1141 atgtccatta tgctttattg aacgccggac tggttattca tgctaagcgt ttcctgattg
     1201 gctattctgc agcaaatgtg aatcaaccta atttcggttt tatggcagaa tcgcgcctac
     1261 ctattcgaca aacgcttcat tctagcttgt gcctttttca aaaagaagga aaacgtaaaa
     1321 gtgctctata cgcgaatgtg ttatacatga aacaacaagg cttccatcag attttaccaa
     1381 gcatttccta tcgctatggt aaatggaaag ctggatgtag ctttcgaaaa ggagatgccg
     1441 ccattggaat gttaggctat acggggaaac gtttgtcaat agcctattcg tacgattaca
     1501 ccatctctac attaaccaac caaacaggtg gcgcacacga attcacgctt ggattaattc
     1561 tcggaaaact caatgaaaaa cgtccatcca tgcgttggac agaggatttg ttttgatata
     1621 aaacgattta cgtattacga tttacgtatt acgatttacg aaaattttgg acggactggc
     1681 attcgttttc atgtgaatta aggaaacggt attcaccacc ctagcaggtt ttctcgtata
     1741 tcgtaaatcg tatagtccct atgggacgat cgtaaatcgg ttctagcagg ttttgtcgta
     1801 aatcgtacat cgtatatcgc ccctcaattc accttcacca ctttccccgt cattactgga
     1861 actccatctt tactcaggcg acaataataa ataccagcag atacgttttc caacgaaatt
     1921 ggagtggatt ctgaaatctg aaattgctga attaattgtc caacactatt aaataattca
     1981 acatttaaaa caggtttaaa atcaccggta tagtgaattt gaaattgcga tgaagtgatg
     2041 gtatttagaa atgaaactga aactggaatg attggtttgg gaatgaccgt aacatttacc
     2101 gtatcccaag tgatgagttt acattgattg gctttgagat aataagaagt agttagttct
     2161 ggatttactg aaatagaatt tcctccgccc aaagaatgta aagtatcgcc ttcgaaccat
     2221 tcgtaacctt ctgtaggtgt ggaagtggag agttgaatag ttgaaccata ataaatacta
     2281 gtgtctgagc ttgaaactaa tgctggtatt tcaataactt caacatcgtc caaataaaga
     2341 taacaatcag cattagtata ggctgtttca ggatttgaga gatcaaaatt tgttatgacc
     2401 tcaaattgaa tatcgttttc attataaaaa ttaccgattg taaaatagcg ttctccacca
     2461 tttgctttgt atggcattac tactttttcc caatttaatg tatcagaaaa gtaggaatca
     2521 ttttcaggtg tgaatgtgat actcggatcc aaataaacgc ttccattcgt aatataattg
     2581 ggccgttcaa gagttatatt aaatcccatt ttattaacga cagagtacag agcgttgtct
     2641 tgattaagca ggtatttata atacaatgtt gatgcttgag aaaggttcat acttacacag
     2701 tattttttat tttctagtgg acttataaat tcactggcaa tgtattctct ccatccagga
     2761 attatattat aggtttgaat gttaaccaat tctacagcaa ttccaatata attgcttcct
     2821 tggtgaggaa tttgaaaccc tcgccaatta aatggtgaga actgatttaa gcatgcatat
     2881 tgttctggtt ctgtttgagg cagcgaaata ggttgattac atgaagaata aacatctgga
     2941 gaacttaaag tcggattaaa ccatccaatt gcatttagat tctgctcctc ggttgtgctc
     3001 tcacaattcg tataacacgt gtcagtttct tcaaaactag gattacgcac taaattctgc
     3061 gcccctaaaa ccaaggagaa taaaaataaa gtgataaata aaatacactt aagcatgcac
     3121 gaatcgtatt tattctgctt aggaactaac tagattcgaa aggtagtgaa agcgccaatt
     3181 tctatatcgt acaattttat ggagatgtcg gtttgagtta aggttgacct ctccctaaat
     3241 ccctctccac aaggagaggg acttgcaatt tcgtatattt tgaagtttca tcgtattact
     3301 cgtatatcgt atatcgtaaa gcgaagcgat cgtaaatcgc cttcccgcct tagggatagc
     3361 agcaaaaatc cttttgtgag gtacgaacaa aagattgtag cggatagccc gggtttgttc
     3421 gccgaagccg gagcgaaggc gaacaaacaa ggcccaaatt attctttctc taggaaacag
     3481 gaactaaacc cggttcatac gccaatactg gacttaacca acgttcggtg tcttcgagcg
     3541 acatgttttt gcgttgggca tattcttcca cttgatcgcg gttgattttt cctaagccaa
     3601 aatacttgct ttcggggtga ccgaaatacc agccacttac tgctgccgtt ggatacatcg
     3661 cataactttc agtaagcgaa atacccgttt gtttgctggc atcgaggagt tggaacagca
     3721 gtcctttttc ggtatggtcg ggacaagcag gatagcctgg agcaggacga ataccgcggt
     3781 atttttcggt gatgagttct tcgttactca aagtttcgtt gcttgcataa ccccagaatt
     3841 cttgacgaac acgttgatga aggcgctcag caaatgcttc tgctaaacga tcggctaaag
     3901 ctttgagcat gatagcagaa taatcgtcgt gattggcttc gaaacgagca acatgttctt
     3961 caattcccaa accagtagtt actgcgaaac cacctatata atcagcaatt ccgctcgatt
     4021 taggtgctac aaaatcgctg aggcaatagt tagcttgtcc agcagctttc ttgttttgtt
     4081 gacgcagatt gtgaaccgtt tctaaaacct gtgtgcgagt ttcgtcggtg tagatttcaa
     4141 tatcgtcgcc tactgaatta gctggccata aaccaatcac tgcattggct tgcaaccatt
     4201 tttcgtcaat caattgcttc aacatcgcat gtgcatcggc aaaaactttg gtggcttcct
     4261 cccctactac tgaatcggtt aagattgcag gatattttcc atacaattcc caagtagcaa
     4321 agaatggcgt ccagtcgatg tattgcgcta attcagccaa atcgtaattc accaaggtgg
     4381 tgtttccaat gtgagctgga gtttgaacat cggatgcttt gaattcaata ggaatgcgat
     4441 tttcacgcgc tgctgataga gaaatatatt ctttcgcaga aacacgtcct gcatgctgtt
     4501 cgcggatttt ctgatattcc acatccaaat cctccatgaa ttttggacgt aattcaggat
     4561 tgataagcga acccgccaca ggcaccgaac gtgaagcatc aagcacgtga accactggtc
     4621 cactatagtt ttgagcaatc tttactgctg tatgtgtttt agaagttgtt gctcctccaa
     4681 tcaacaaagg aattttaaac ccttgttttt ccatttcacg ggcaacggtc accatttcgt
     4741 cgagcgatgg ggtgatgagt ccgctaaggc caataatgtc ggcgccaatt tcacgagctg
     4801 tgtccaggat tttctgataa ggaaccatca ctcctatatc aaccacttcg taattattgc
     4861 agcctaaaac aacacccaca atgtttttcc caatatcgtg aacatcgcct ttcacggtag
     4921 ccatgaggat ggttccgttg ctgttcgatt caccttctct tttttcggct tcgatgaatg
     4981 gtagcaaata cgccaccgat ttcttcatta cacgcgcgct ttttaccacc tgcggaagaa
     5041 acatttttcc actaccaaac aaatcaccca caatgttcat cccgtccatc aaaggacctt
     5101 caatcacctg caaaggacga tcaactagtt gtcgagcttc ttcagtgtct tcgtcgataa
     5161 attccacgat tcccttgata agcgcatggc tgaggcgttt gttcactggc caagttctcc
     5221 attccaggtc tttctcctga acttttcctt tcgacttgat ggtttcggca aattcaacca
     5281 aacgttcggt tgaatcggga cgacgattga gcaatacatc ttcaaccagc tcgagcaaat
     5341 ctttcggaat ttcttcgtag acctctaatt gtccggcatt gacaattcct atatccaatc
     5401 cagccttgat agcgtgataa aggaatgccg agtgcatagc ttcacgaacg cgatcgttac
     5461 cacggaacga gaaggaaata ttagaaacac cgccacttac acgcgctcct ggaagattat
     5521 tttttatcca gcgagttgct ttaatgaaat cgactgcata gttgttatgt tcttcgattc
     5581 cagttgcaac cgttagaatg ttgggatcaa agatgatatc tgtaggtgga agtccgactt
     5641 cgtttactaa gatgttataa gcacgttggc aaatctcaat gcgtcgttcg tatgaatcgg
     5701 cttgaccttg ttcatcaaac gccatcacca caaccgcagc tccgtatcgt ttcacgagtc
     5761 gtgcatggtg acgaaaagca tcttcccctt ctttaaggga gattgagttt acgattgatt
     5821 ttccttgaac gcatttcaat cccgcttcaa tcaccgacca tttagatgaa tcgatcatga
     5881 aaggaagttt ggcgatatcg ggttcagaag ccatgaggtt gagaaacttc accatagcag
     5941 cttccgaatc gagcatgcct tcatccatgt tcacatccag aatttgggca ccgccttcta
     6001 cttgattgag cgcaacagta agggcttcat cgtaattgcc ttccatgata aggcgagcga
     6061 atttcttaga acccgtcaca ttggtacgtt ccccaatatt gacaaagtta gtttcctcgc
     6121 ggatagtaac cggttcaagt ccgctgtaga atggaacagg ctccacatca ggaataacgt
     6181 gtggtttata ttcggcagct acacgagcaa tttcacgaat gtgatcagga gtagtaccgc
     6241 agcaaccacc aatgatgttg atgaagttat tttcgaggta ttcacgaatt tgaacaccca
     6301 tttgctcagg tgtttcatcg tattgtccga aagcatttgg caatccagca ttcgggtgag
     6361 cagaaatgcg gaatttggaa ttcttagcga gtgtttcgat gtaaggcttc aattgacgcg
     6421 caccaagggc acaattgaat ccaacagaaa gcaaatcgat gtgcgaaaca gaatagagga
     6481 atgcttcggc tgtttgtccg gttagtgttc taccagaagc atcggtaatc gttcccgata
     6541 ccataacagg catgcgaatt ccttgttttt cgcaatattg gtcgatggca aaaagtgccg
     6601 ctttacaatt cagtgtatcg aaaacggttt caacaagcag aatatcaacg ccaccatcaa
     6661 taagacctcg tacttgctcg gtgtaggctt ccacaagttc atcgaatgtg atggcacgat
     6721 agccaggatc gtttacatcg ggagaaatgc ttgctgttcg gtttgttgga ccgattgaac
     6781 cagcaacaaa acgaggctta tcgggttgaa gggcggtgta tttatcagca gcttgacgag
     6841 ctaaacgagc agcagctaca ttgaggtcgt aaacgatatg ttcgaggcga taatcggctt
     6901 gcgcgatgct ggtaccgctg aaggtattgg tttcggcgat atcggcaccg gcttcgtaat
     6961 aggcttcatg aatcccaaga atgatttcgg gacgcgtgag ggcgagtagg tcgttgttgc
     7021 cttttagagg agaaggatga ttttcgaaat gacctttccg gaaatcttct tcttccagtt
     7081 gatgacgttg aatcatggtt cccatggcgc catcaaggac gagaatacgt tttttcagtt
     7141 gctcctgtag taatgacata cgtgtatctt tcttcgcgtg aagaacacta acagaaagat
     7201 ttggttgccg caaacaggaa tttgaattga tggttcattc ttatctgctc cccaatgggg
     7261 ctaggaattg gcacctttct caaaagaggg ttgccaagac gtcttagggc ctattccctc
     7321 ggtctttctg gataagtgta cttcaatgaa cgtttattca ggggcaaaag tacaaaaata
     7381 ttcatcaagc aagcaagcgt acccggttca tatcacgttg aagtgcaatc ttgtcgttat
     7441 caagcttctg atatcctatt tcgtagcaaa aaagggtcac gtctccattg ggattaatgg
     7501 attgatgggt gaaatatcga aaatcgaaac cacgagcact taagtaggaa gatgcaactc
     7561 ttctcacacg accttgaaaa caggtattga ggattttcct attccgcgat aagatgaggt
     7621 tgacttcttt caccgcttca ctttcggaaa tacgttcccg gttgtgataa acattccggc
     7681 ataaatcaga acagaatcgt ttgtcgctac gcccatggac gatttcgccg cattcggggc
     7741 aatttttagt tgaatacata agattatata tagatagaat taatgatacc caaacttagt
     7801 gtatgagaat taaaaagtca agacagattt tcaattttcc caaaagccgt tttaaacgtt
     7861 tatatacgta tataaatgtt taacagacga ttagttgaaa atcgccctcg tatgtttgtc
     7921 ctgtcaattg ataaccggaa cgaacgggtg cacccatttc ttttatcatt taattattaa
     7981 acgtatgaac aacttaaaaa actctgtccg cctgatagga aatctgggtg gcaatccgga
     8041 agtgcgagaa cttccaagtg gaaagaagct agcgaaattt tcgttagcaa caaccgacaa
     8101 gtttaaagac agtgaaggaa agctggttaa tgaaacccaa tggcataatt tgattgcttg
     8161 gggaaaaaca gcagatttca ttgaaaaata tgtcgaaaaa ggtcaagagg tagtaataga
     8221 aggtaagctt caaagtcgat cctattcgga taaagaagga gcgaagaaat acattactga
     8281 aatctctgtg aatgaaattc tgaaaatagg aaacggaaag agaaaggagt agttcaaccg
     8341 aggaaggaaa tgctggtttg gtccagcttt ccttccattt tcaaacgtat ttggaattat
     8401 tctaaatatc gaaatcgtgt aagaaggtac gtgaatttga atgacaaaag tgcaactcct
     8461 aaatggttaa cacgtttaga attgtgataa ctcacgtcat gttaagtcct tcaatctata
     8521 gttctatttt gcccagcctg attattctgg taggaatagg aatgttgttt agaagcctaa
     8581 aacttgggta tgagatacag agggtttcag aagaagtttc acaaaagcgc tggaagctga
     8641 ttaatagact tatcaaagga tttattgtat tttatggaat tgcaatatgt gtttcctggt
     8701 tgtcattcca gccatgggta atattcgttt ttgctttgtt attattggga ggagcatact
     8761 ttgttttcaa aatccttcaa ttatccaagt ttagcttatt gacatttgta aaaacgcatc
     8821 attctcaagc atttttgatg gagattttcc aaacgatggg aaatgcgtta attgttgctg
     8881 ataaagaagg gatgatcacc ttggtaaacc catgtttaac aagacttata ggaattcctc
     8941 aagaagaaat tctaggtaga aaagcagaag aactttttct cagtgagatt agtgatatgc
     9001 cgggagtgca acatgagaat ggtttgttac taccatcact caaagaaatt cctgtattgg
     9061 taacaatttc gcatacgtat cagcaaggaa atttgataca aattattgta ttacaggata
     9121 ttcgaaagca aaaggaaaat gaaattcgtt taaagaagta cttacaaaag atagagcaga
     9181 ataatctgga gcttgaacaa ttctcctatg tagcagcaca cgatttaaaa gctccattac
     9241 gagctattca aaatcttact gagttcattg aagaagatat gaagcaagtt ccatcgggtg
     9301 tacaacagca ctttcagctg atcaagagcc gtatcgcaag aatggagaac ttgattaatg
     9361 gattattgga atatgcgcgt gttggtaaga aaggtgtgaa tctcgaaatc actaacgtaa
     9421 aagaattact ggaagatatt gtagataatc ttggtgtcga gaaacgttct aggatggaga
     9481 tacaatcgga attaccaact ttgttcaccc ataaattatt actagagcag gtttttgcga
     9541 atttgatcag caatgctcat aaatacaata cgaatccgag tccagtgatc aaaattggat
     9601 cgcgtgatga tgggaagcaa tttgagtttt cggtaagtga taatggacct ggtattccag
     9661 ccgacagcaa agagaaaata ttcaagattt ttcaaacact acaagcgcgc gatgattacg
     9721 aaagcacggg agtaggatta tcgattgtaa agaaaatagt agaagaaacc gggtgtgaaa
     9781 tccatgtaga ttcagaacca ggtgaaggtt gtacattccg ttttacctgg ccgagatcgg
     9841 gtgcagctta gtttgtagaa attgggcgat catcccaaac tttagcacca tcgagtagat
     9901 aatcttctat acgggcttca cctattttga tacttacttt gtcgattaag aatcttactt
     9961 cgcagcgatt tttatccgga acagttgagt cggtattacg agttgtccct tctatatatc
    10021 cgatataagt aacattccca tgtatggtat caacttgagc tagctcgatt gaataccatt
    10081 tttccttttc gccagcattc acgtgaatta catttttctt cttgttccaa tagattgaat
    10141 attcgactgg tttaacacct tcggagaaca ttttactttt cccgattaat gatgtacgtg
    10201 ttttagtacc agtgtattca aatccttttt tactgtgttg agcaaaaaca gcggtagaaa
    10261 gtgaaaagaa aaggatgaga agtgtcgcgt aatttttcat aggataggca aaagtcaaaa
    10321 ataataaatc aaacgatgca acgtgtatgg ttatcaatta agcgagaatt atttacacag
    10381 gtatattatt ttatgtattt taagccgaac tgaaatatcg ggctatgcga gtaaaacctg
    10441 aaataaaata tggcctatta ggtgcgttat tactcattat ttggactctc acccaatatt
    10501 ttgctggctt tcatacttac cattttgcta tagctcaata cacaggttat ggtaatttct
    10561 taatcgtttt tatttgtctg tggtttggat taaaggagaa aagccgtgac tataaaggtc
    10621 aattttcagt aagacgtggt gtaaaagaga gtatgtatca actatttata acagcttctt
    10681 tagcatcttt atttatgttc ttatatgact acaagataaa ctcactttgg gtagaggaaa
    10741 tggttacttg gcaacgtcaa aacagcagtt ctttttctct taatctattt ctagctaacg
    10801 acccacatgc cgaagctatt atactaagta atacagaaac ccatttatgc ctatactttt
    10861 taggaatagt ggttgttggc gcaagtatgg cttttatgat ttctgctatg atttcgaaat
    10921 caaaacctag caatgcttaa ataaaaaagt gcttgattaa aatttcaatc ctgcctcgat
    10981 tttgagacct aagtctgatt tgtaaacttc tgacttaaaa aagcgctcat ataatcctgc
    11041 tccaaggtag aaaccgatcc tagaacgtgt cataaaataa aaactctggg taggttcaac
    11101 accataaatg aagtttgtgg tttcagtata agtaattgct gagcctactt gatttttcct
    11161 gactaattct tccactccta aaggaatatt tacattgaat agaaaatgga agtcgcctcc
    11221 tatcttcttc atagctccta atccaatttt tccataattg agtttagctg tttcatagcc
    11281 atttctcaga ataagtcctg gatcaatcac atatttatcc atggcaaccg aaattggaaa
    11341 tgcccaactt cctttcatca cagaagaaac tcccaaataa gataaacccc atccctcggc
    11401 attcgatcca aaagcagcat gtgcttgaat taaacttgtc cacgttttct tctctggaac
    11461 tagtgcattg tttaataaaa tacttttctg tcctcgaatc tcagcatatt ctatgaattc
    11521 aggctgaaca gttttccaat tactgttatt gaaagaaaga attacacttt ctaaaacttt
    11581 tcgaagtcgt cgttcgtgtc ctgaagtaac atctggagca acttcctcgg tttgtgcttc
    11641 cgtttcgaat actttacccc atttaccttc tgcattcggc ttatagaatt caactttcaa
    11701 ctcagctttc ccaatttctg aaggaccatc agttcgttcc gaaacttgca agaaaactac
    11761 acgaataaca atcggtatca ggctcgtatc aggtgttaca gcatacgtgt aaaagtcagt
    11821 caaggccttt tctaatccac cttgcaactc cacatgttct tttttattga aagaaccttt
    11881 gtgcacataa ccaatatcgg aagtatgacc acgaccatct atcaccgatt tgatatagaa
    11941 cttctgatca gacatgcgaa tcatgctgtt tttaagaaca atcacttctt tttgagcaaa
    12001 agcagatgct gaaacaaaaa gtaaaaatag gacagcggaa attcgtctaa tcacaagagc
    12061 ggaataaatt caattaaggg cttcaaaagt aacatgccga ggataataaa caatacccaa
    12121 agtggtactt aacgtttcga cgcaatatag gtactcaaac tctgacggtc ggaactggca
    12181 ggtaaaagtg ggcttatttc aaagaagtaa ttctccttat ctgaaacata atcataagca
    12241 aacttggaaa ggattaactt atcggttgat gaactgaata aagcaactaa ttctttaatc
    12301 tgacgcgatt tatagcaatt gaatctcgga atttgttgtc gggcaatggt taatcgtgtt
    12361 gaagaagagg attcgtttgc gagaggttct ttaatcgttc taaaatcatt gtcgtttact
    12421 atgcaaggag gcggaggcaa ttgagcctgt gcgtttttac gactaacgac taaaaattcc
    12481 tttaaagcag cttgattggg aacgtgtgta aaaacctgag tagcaaaatc gaagtttgct
    12541 tcatcataaa ctcgcagata ggccttcttc aacaattcta agcgattgtt ttcaatgttt
    12601 aaagctgttg ccaacttcat gctttgagaa gtcgttaagc acgttcttgt tgctaaagac
    12661 agtgctgctt cattcttctc ctgttctgat ttatgggtag cgatctcacg aacaaacatc
    12721 ataaaatcgt tatctgataa ggaagtcaca caattctttg gtccgttgta attagctgca
    12781 tccggataat ttaaaacggg aaagttaatt tcaagtggct taggcggaat cggattacta
    12841 ggtacaacag ttaccggttt ttgacttgta gcaatgtagt cgtgaagacg gaaagcactt
    12901 gagaaatagg caaacgagtt atagatatcg tatacatctt gcttattgag caagcgagga
    12961 tatgctgcca ttgctaaaga aagacgatcg ttctcttcgg atatataacc cagcaattca
    13021 attaattgat aggtagtgaa acattctcca cgaacaaaac catccacata ggtataacgc
    13081 t
//
LOCUS       DAOOQW010000048        12996 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_5922_length_12996_cov_5.018932, whole genome shotgun sequence.
ACCESSION   DAOOQW010000048 DAOOQW010000000
VERSION     DAOOQW010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 12996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12996
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5922_length_12996_cov_5.018932"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1283)
                     /locus_tag="PK281_04555"
     CDS             complement(<1..1283)
                     /locus_tag="PK281_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48648.1"
                     /translation="MLLLNVRPLLADGGARFEQNKGQWPQQVRFRAEIPGGALFLEKQ
                     GLTYHFQKSNHEGHQHGTNEQEPELIQGHVIRMELIGSENEARFSGEKQLPYYSNYYI
                     GKDRKNWASNVSVFEKVISKHIYSGIDWIIYSNQTGLKYDFEIAPNANPGLIKMQYSG
                     ADKMKVVDGKLHIWTSLGMLEEHAPIAWQIIDGEKKDVSCKFELNNQEVSFKLGKYDS
                     NFPLIIDPQLVFGSFSGSTADNWGFTATYDNAGNTYSAGVVFGVGYQTTTGAYQQNFA
                     GGSGSRPCDIGIIKYNNAGQRLFATYLGGAGNEIPQSLIVSSSNELFLFGTTGSNDFP
                     TSSTAWQRTFGGGTDVNILNSGIKFPSGTDLFVSRLSENGTQLLASTLLGGSGNDGLN
                     TASQLRYNYADEARGGIAIDAQNNVYCAATTDRLG"
     gene            complement(1376..2506)
                     /locus_tag="PK281_04560"
     CDS             complement(1376..2506)
                     /locus_tag="PK281_04560"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY48649.1"
                     /translation="MKKRLLPTLLILSSVQVFSQVTVNQADMPSAGDTLRFSVANTSG
                     IDAGSGGSNQTWDFSNLQSTSQFVDEFLPVSSTPFTYVATYGFPFSSSYSDMGRLDNS
                     AFQIPTIPIPGFEISITDVYNFYKSNSGDFSQMGFGASINSFPVPIPYSDADVLVPLP
                     LTSSSTNSSAYSFNIQIPTLGYYGRQAVRNNEVDGWGTLITPFGTFQTLRVRSELVYT
                     DTLAIDQLGFGFQLPEQSEIKYKWLAPGYGWPLLEITATVLPFIGTETISRVVYRDGV
                     EDQTGLNDQLQSTDVLLYPNPASDVAIVNAHLKKASTMSYTILDAIGRVISQVNRGVQ
                     AAGTCMEILPLTQLNLADGMYFIKIQAGTDRPVVRPLHVNNH"
     gene            complement(2548..2982)
                     /locus_tag="PK281_04565"
     CDS             complement(2548..2982)
                     /locus_tag="PK281_04565"
                     /inference="COORDINATES: protein motif:HMM:NF021435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2141 domain-containing protein"
                     /protein_id="HOY48650.1"
                     /translation="MSILITPYLFLLTLIHSSVSVPSDMKIEISGIQNAKGHILIAVF
                     NQADGFPDESSKAFRKERIPAKSGSISYVLTGLPAGKYAIGIVHDENDNMKLDTGLFG
                     IPKEGFCFSNAAMGTFGPPSFEAAAILHPSKNQPHKLIVKYW"
     gene            3058..3669
                     /locus_tag="PK281_04570"
     CDS             3058..3669
                     /locus_tag="PK281_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48651.1"
                     /translation="MNSPFLAFLEGKEIPGFALLKNTRIENVARKGLRIAHFDEAHWS
                     ESFDVLKAMLPVATLLPTREAREQAAYKFSEEDLKASHFHFDDMPLALTNESSDRELF
                     GECYTIFWVVPANKEIIISQCLADLSSADKITGDHLKKLWDEKVNRIFFHFTGELDVV
                     EHTLLQDELHDELIEAINALPNAPEESAWDALNEDVFWDDDDY"
     gene            3656..4444
                     /locus_tag="PK281_04575"
     CDS             3656..4444
                     /locus_tag="PK281_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010232150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HOY48652.1"
                     /translation="MTTIKPFSNSELKALLDEKADTYNRTSFIETDPISVPHRFVHPF
                     DIEIAAFLAATIAWGQRVTLIRNANWLLDRMDQAPADFIRNLQEQDLKPFQKFVHRTF
                     NGEDCLNFLRALNRISHDYKSLEHLFKSDNGDVKSGIIQFRSIFLADEIVSRTAKHVS
                     DPAKNSAAKRLNMFLRWMVRKDVRKVDFGLWTVFNPSQLICPLDVHSGGVARKLGLLQ
                     RKQDDWQAAEELTAALSRFDPVDPVKYDFALFGLGVFEEFRKLP"
     gene            complement(4441..5160)
                     /locus_tag="PK281_04580"
     CDS             complement(4441..5160)
                     /locus_tag="PK281_04580"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOY48653.1"
                     /translation="MKRKLPYIFLGVIGGLMSGADLNAQVDPVSLGQVGLDQKVADVD
                     LLAELNEEYSTPKAKTPAKSLDLEIKASNLTIDLKKNKQVEFSARTAWATNFVWKFGD
                     GSTMSGFQHVKHEFKKPGKYEVTLLASNKEQVAKQTIEVNVVDNSAPLELEEMEHYIV
                     FPANNSLEVDIQLNLPRKEKHLLLEVKNVEGERVFEYEVGKVRKTELIHVDLQNLEAG
                     KYYAVLKGRKYSLVSRLTVAR"
     gene            complement(5499..6638)
                     /locus_tag="PK281_04585"
     CDS             complement(5499..6638)
                     /locus_tag="PK281_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008238261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOY48654.1"
                     /translation="MDFQLNENQKMIAEMIRNFGDKHIRPNMMEWDETQHFPREIFRQ
                     LGELGLMGVLVPQEYGGAGLSYTEYVTAIIELSKIDPSIGLSMAAHNSLCTGHTLMFA
                     NEDQKKKFLPKLASGEWIGAWGLTEPNTGSDAMRMKCVAHQDGDYWVINGTKCWITHG
                     ISAELAVVIVRTGELLDSHGMTAFLVERGTPGFMAGKKENKLGMRASETAEMIFDNCR
                     VHKDQVLGKVGDGFIQAMKILDGGRISIAALSLGIGKGAIEASVKYSKEREQFGQPIS
                     NFQGISFKLADMSTRYQASELLTFYAADLKNRGQRMTTESAMAKLHASEMCVYASNEA
                     VQIHGGYGYTKDFPVEKYYRDSKLCTIGEGTSEIQKVVIAKNILQ"
     gene            complement(6856..9048)
                     /locus_tag="PK281_04590"
     CDS             complement(6856..9048)
                     /locus_tag="PK281_04590"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY48655.1"
                     /translation="MRISLLISSIFLTCSGILAQSTQTILALDRNDDIPVFSIDSIQL
                     KFPTTGGFNYMQAGAIDLNNDGVQDLLMFDRTGDRILPFIYTGTPGNPDYRFEHSLVY
                     SFPKVKDFLIVKDFNCDGKADLFTYENSGFKVYKNISSASGLQFELYTESLQSYFNPA
                     TLAVFCIPVDVPVVEDMDNDGDQDLLVFGILGTCVEYHRNMAVEQLGRCDTLMLKLES
                     DNWGLFTESFSTNDVVINDSCDHPTGRNNALRHAGSTMLAYDADGDGDKDMLLGDIAY
                     RTMTQLINGGNANYGQITSSQTPFPSGTPYINLPIFPAAFFLDYDHDGVRDLIVSPNN
                     EGGSENHMCIWYYHNDGTDAIPVFTFVKKDLLLDESLDFGEGCIPTFFDYNGDGLQDI
                     VIGNYGYFQPAGTYKSQLALLKNISTDTSPKFKLVDTDFAGLAQLTGNTTNYHPSFGD
                     VDGDSDQDMVLGTSDGRIFYYINEAAPGNDANFVFSSPNFQGIDIGTYSAPQLIDVDT
                     DGLLDMLIGCREGTIHYYKNFGTPTNMILQEVNTTFGQVSTAENGDPNGFSTPTLFRK
                     SGVSYLLCGSLSGRFHLYSGIDGNLSGAFVLEDSLLLGTSDGERTSMSLKDLNDDGFL
                     DAVTGNYAGGINFFSGVFPSSLENKTAQEPNFVIYPNPGSDLFVRSLQEKIKQIEIFD
                     MQGRMVHFQSGTNQPTVALTDLQLDAGIYFVRVKGAQNTQTFKWIRKK"
     gene            9272..12283
                     /gene="secDF"
                     /locus_tag="PK281_04595"
     CDS             9272..12283
                     /gene="secDF"
                     /locus_tag="PK281_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010228664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HOY48656.1"
                     /translation="MQNKGAISFFAIMLALVCLFELSFTWKASSVESDAKIFAAGDPK
                     KEKAYLDSIASETVYNVLFRKYTYRECKAKELNLGLDLKGGMNVTLEVAVSDVVRSLS
                     NNSTDPTFLKAMELANARQKSNGGDFIDLFAAAVKETDPAARLSSPAFFGNKDLKDLV
                     NSKMTDEEVIKVIRTKVKSSVDNAYTVIRNRIDKFGVTQPNIQRLEGSDRILVELPGV
                     KEPERVRKLLQGAAKLEFWETIENKDAFQYLEAANKRLKDITNAGTAPAAVPAVTDTT
                     AAATPVDTTATVAKDTSLSLAEKLAKADTTSDKDTSAAAQRARAQKDNPLYVVLSPNI
                     NQTNEGSTFRPGPVVGISLTKDTSRVNQIFSMPQIKEVFPKDIRFFWSVKPFDEKENA
                     FELVAIKVPRDGKPRLTGEYVTDARLSYGQLNNKPEVTMAMNGEGAGIWKRMTGENVG
                     KSVAIVLDNYVYSYPTVQGEIAGGNSSITGNFTIKEAEDLSNILNSGKMDAPARIVEE
                     AVVGPSLGAEAINSGLLSALAGLILVFLFMGFYYSGGGIVADVALIANVFFLMGVMTS
                     LGAVLTLPGIAGIVLTIGMSVDANVLIYERIREELASGKAYKQAIADGYKGAYSAIID
                     SNVTTLLTGIILYIFGTGPIQGFATTLVIGILTSLFSAIFITRLCFEFLGKRNKEVKF
                     SIPMTAKLFSGSNFDFINQRKRFYIVSLAVIVIGVGFMVTKGFGYGVDFEGGRSYVVR
                     FDKPVQTDQAREVLYKAFQKSPDVKVYGSSNQLKITTGYMLEDESLAADSIVASKMLS
                     ELTPITGAVPVIQSSQKVEPTVANDIKDSAFLSVIFSLLVLFLYLLLRFRRWQYGIGA
                     IAALAYVVIFILSIFSIFNHILPFSLDIDQAFIAAILTVVGYAINDTVVIFDRIREYL
                     AMPNSKREDLSTIINNALNSTLSRTAVTGLTTIGVLIILFIFGGEVIRGFAFAMLIGV
                     IVGTYTSLFVASPVVVDLTRRVAGKSDEEKK"
     gene            12335..12751
                     /locus_tag="PK281_04600"
     CDS             12335..12751
                     /locus_tag="PK281_04600"
                     /inference="COORDINATES: protein motif:HMM:NF014470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HOY48657.1"
                     /translation="MLASVYLFFDVSGGEITVIILFILIFFGSKKIPELARGLGKGMR
                     EFQDASNSIRREIQTEANKIKDEVSKVQTQVREEVKLDQLTEDAPNTPKPLDSVAHVA
                     TPEPNPSVVTDESATNQTTNPEAAEIKSELPTTSQI"
     gene            12748..12957
                     /locus_tag="PK281_04605"
     CDS             12748..12957
                     /locus_tag="PK281_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006257970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HOY48658.1"
                     /translation="MIQGHIFLGALGPSEIILIGAGILLLFGGKKIPELMKGLGQGMR
                     EFKDAKDGKTELPEANKEKQTQINS"
BASE COUNT         3857 a         2662 c         2558 g         3919 t
ORIGIN      
        1 aatcccaacc ggtcagtggt agcggcgcaa tacacattgt tttgtgcatc gatggcaatt
       61 ccgcctcgtg cttcatcagc ataattataa cggagctgtg aagctgtatt taatccatca
      121 tttcccgagc cgcccagtag ggtagatgct aatagttgag tcccgttttc tgataatcga
      181 ctgacaaata aatcggtacc acttgggaat ttaattccac tatttaaaat attgacatca
      241 gttccaccgc caaaagtgcg ttgccaagca gttgaactgg taggaaaatc gtttgagcca
      301 gtagttccaa acaagaataa ttcattgcta ctacttacaa ttaaactctg aggaatttca
      361 tttcctgctc ctcctaaata ggtggcgaaa agacgttgtc cggcattgtt gtatttgata
      421 attccaatat cacacggacg agaaccgctt cctccagcaa aattctgctg atatgcacct
      481 gtcgttgttt gatacccaac tccaaataca actcctgctg aataagtatt tccagcattg
      541 tcgtatgtgg cggtaaatcc ccaattatcg gctgttgatc cggaaaagct tccaaaaacc
      601 aattgtggat cgataataag tgggaagttg gaatcgtatt ttcctagttt gaaacttact
      661 tcttgattgt tgagttcgaa tttacacgac acatcttttt tctccccatc aatgatctgc
      721 caggcaattg gggcgtgttc ttccagcatt cccagtgaag tccagatgtg taattttcca
      781 tctaccactt tcattttatc ggcaccggaa tactgcattt taattaaacc cggattggca
      841 ttgggcgcta tttcaaaatc gtattttaat cctgtttggt tggagtaaat gatccaatca
      901 attcccgaat aaatatgctt gctaattact ttttcaaaaa cagaaacgtt ggaagcccag
      961 ttcttacggt cttttccaat gtaatagtta gaataataag gcaactgttt ctcacccgaa
     1021 aaacgggctt cattttcgga gccaattaat tccattcgaa taacatgtcc ctgaattaat
     1081 tcaggttctt gttcattcgt gccatgttga tgtccttcgt gattactttt ttggaaatga
     1141 taggttaatc cttgtttctc gaggaatagt gcaccgccgg gaatttcggc acggaaacga
     1201 acttgttgag gccattgtcc tttgttctgt tcaaagcgcg cgcctccatc agccaataaa
     1261 ggtcgaacgt tcagcaatag caaaaaacag aataatacta accgagtact cgtttgcatt
     1321 ccatataggc tttttcaaaa atacctgctt ggaatgcttg tacgaggatt aggacctaat
     1381 ggttattaac atgcaatggt cgaacaaccg gacgatcagt accagcctga atcttgataa
     1441 agtacattcc atcagccaag tttaattggg ttagtggaag tatttccatg catgttccag
     1501 ctgcttgtac tccacggttg acttgagaaa tcactcgtcc aatagcatct aatatcgtat
     1561 acgacatagt gcttgctttc ttcaaatgag cattcacaat agccacatcc gaagctggat
     1621 ttgggtaaag aagtacatct gttgattgaa gttgatcatt taatccagtt tgatcttcta
     1681 ctccatcgcg atataccaca cgtgaaatag tttcggtacc aatgaaaggt aatacggtag
     1741 ctgtaatttc gagtaatggc catccataac caggagctaa ccatttgtat tttatttctg
     1801 attgctccgg aagttgaaat ccaaagccta attgatcgat tgctaatgta tcggtataca
     1861 ctaattcgga acgcacacgt aaagtttgaa aagtaccaaa tggagtgatt aatgttcccc
     1921 aaccatctac ttcgttattt cgaactgctt ggcgaccata atatccaaga gtaggaattt
     1981 gaatattgaa gctgtatgcc gatgagtttg tactggaaga tgtaagtgga agtggaacta
     2041 acacatctgc atcgctatat ggaattggaa ctgggaagct attgatgctg gcaccaaagc
     2101 ccatttgaga aaaatctccc gagttacttt tgtaaaaatt gtacacatcg gtaatggaga
     2161 tttcaaatcc cggaattggg attgtaggaa tttgaaaagc tgaattgtct aatctaccca
     2221 tatccgaata gctgctacta aagggaaatc cataagtagc tacataggta aatggggtag
     2281 aggaaacggg aagaaattca tctacgaatt gagatgtaga ttgcaaattg ctaaaatccc
     2341 atgtttggtt gctaccgccc gaacctgcat caataccaga tgtatttgca acactaaaac
     2401 ggagagtgtc gccagcagat ggcatatcag cttgattgac agttacctgt gaaaatactt
     2461 gtactgaact cagtataagg agtgtaggaa gtaaacgttt cttcatgtag ctagtttgtg
     2521 ctggcaatat acactttcag cacatcctta ccaatactta actatcagtt tatgaggttg
     2581 atttttacta ggatgtaaga tagcagcagc ttcaaatgat ggcggtccaa atgtgcccat
     2641 tgctgcattg gagaaacaaa atccttcttt gggaatacca aacaaaccgg tatcaagctt
     2701 catattatca ttttcatcgt gcacaattcc aatggcatat tttcccgcag gaagtccagt
     2761 caatacataa gaaatacttc ccgatttggc tggtatacgc tctttgcgaa atgctttaga
     2821 tgattcatcc ggaaaaccat ctgcctgatt aaacacggca atgagaatgt gacccttcgc
     2881 gttttgaatt ccgctgattt cgatcttcat atcggaagga accgaaacag aactgtgtat
     2941 caatgtgagc aaaaacaaat acggtgtgat taaaattgac atactcgaaa aacagaaact
     3001 tttacttatt gttcaaggca caagctaaat caaattcaat tgatattttt gccggatatg
     3061 aacagcccct ttttagcatt tctcgaaggc aaagaaattc ccggttttgc cttacttaaa
     3121 aatacacgaa tcgaaaatgt agcccgtaaa ggtttgcgga ttgctcattt tgatgaagcg
     3181 cattggtctg agtcgtttga tgtattaaaa gctatgttgc ccgtggctac tcttttgccg
     3241 acgcgtgaag ctagagagca agcagcttat aaatttagtg aggaagatct gaaagcatcg
     3301 cattttcact ttgatgatat gccattggct ttaaccaacg aatcgagcga tcgtgaatta
     3361 tttggtgaat gctacactat tttttgggta gttccagcga ataaagaaat catcatatct
     3421 cagtgcttag ccgatctttc ttctgccgat aaaatcacag gagatcatct gaagaaattg
     3481 tgggatgaaa aagtgaatcg aatttttttc catttcactg gtgagctgga tgttgttgaa
     3541 cataccttac tgcaagatga attgcacgac gaattaattg aagccatcaa cgccttgcct
     3601 aatgcaccag aagaatcggc ttgggatgcc ctaaatgaag atgttttctg ggacgatgac
     3661 gactattaag ccgttttcaa attccgaact taaagcattg ctcgacgaaa aagctgatac
     3721 atacaatcga accagcttta tcgaaacgga tcctatttca gtaccgcatc gctttgttca
     3781 tccattcgat atcgaaattg cagccttttt agcagccaca attgcgtggg gacaaagagt
     3841 aactttaata cgtaatgcca attggttgtt agatcgtatg gatcaagcac cagctgattt
     3901 cattcgcaac cttcaagaac aggatttaaa gccgtttcag aagtttgtcc accgtacgtt
     3961 taatggggaa gattgtttga atttcctaag agctttgaat cggatttcac acgattacaa
     4021 atctttagag cacttattta aatcagataa tggggatgtg aaatcgggta tcattcagtt
     4081 tcgatcgatt tttctggcgg atgagattgt ctcaagaacg gccaaacacg tgtcagatcc
     4141 agcaaagaat tcggctgcaa agcgcttgaa tatgtttttg cggtggatgg tgagaaagga
     4201 tgttcgaaaa gtagatttcg gactttggac agtttttaat ccaagtcaat tgatttgtcc
     4261 gctcgatgtt cattcgggtg gtgttgcccg taaactcgga ttgcttcaac ggaaacagga
     4321 cgattggcag gcggcagaag aattaactgc agcactaagc cgattcgatc cggtagatcc
     4381 ggtgaagtac gatttcgccc tatttggatt gggcgtattt gaagaatttc ggaaactgcc
     4441 ttaacgagca acagtcaagc gactaactaa gctgtatttt cttcctttca aaacagcata
     4501 atattttcct gcttctaagt tttgaagatc aacatggatg agttcggttt tacggacttt
     4561 cccaacttca tattcaaaaa cacgttcgcc ttccacgttt ttcacttcta ataagaggtg
     4621 tttttctttg cgaggtaggt tgagttgaat atcgacttcg agactgttat tagctggaaa
     4681 cacaatataa tgttccattt cttccagttc gagcggagcc gaattatcca ccacattcac
     4741 ttcgatggtt tgtttggcta cttgctcttt gttgctggct aataaggtaa cttcgtattt
     4801 acctggcttt ttgaattcat gttttacatg ttgaaagcca ctcatagtgc ttccatctcc
     4861 aaacttccaa acgaaatttg ttgcccaagc agttcgtgct gaaaattcaa cctgcttatt
     4921 cttctttaag tcgatagtta gattcgatgc tttaatctcg agatctaacg acttggcagg
     4981 agttttcgct tttggagtgc tatactcttc gtttaattcg gccaacagat caacgtcagc
     5041 tactttttga tctaatccga cctgtcccaa agaaacagga tcaacttggg cattcaaatc
     5101 agctcctgac atcaggcctc cgataactcc caaaaaaatg taaggtaatt ttcgtttcat
     5161 aattgctaca cattggtgca tagcaaacct agcttgggta tatcgctcaa ctagaccatt
     5221 gcaagccgac taccgaagca tgtaagagaa acgttttatt ggggaaataa aaatggatat
     5281 aattaactta atgtattgat aatgaaattg ttgaaatatt gaatattttt atttggtatt
     5341 caatatcatt tgaaaaatga actgtaaatt tttgtaaccc tgaaaggtta catcaaattc
     5401 aaaaaatccg tttgggcaga taaaaagtat caaacgtgaa aaagccgcat ctgcttagag
     5461 caaacacggc tttttttggg aaacagagat actgtttttt attgtaggat attcttagcg
     5521 attaccactt tttgaatctc ggaagttcct tctccaatgg tacatagctt agaatcgcgg
     5581 tagtattttt ctactgggaa atctttggta taaccatatc caccgtgaat ctgaactgct
     5641 tcgttagagg cataaacaca catttcagat gcatgcaatt ttgccattgc cgattcggtt
     5701 gtcatgcgct gaccacggtt ctttaaatcg gctgcgtaaa acgttaataa ttcagatgct
     5761 tgatatcgag tactcatatc ggccaacttg aagctaattc cttggaaatt gctgatgggt
     5821 tgtccaaact gctcacgttc tttagaatac ttgacagaag cttcaatcgc accttttcca
     5881 attcctaagg ataaggctgc aattgaaata cgaccaccat cgagaatttt cattgcttga
     5941 ataaagccat cacccacttt tcctaaaact tggtctttgt gaacacggca gttatcgaaa
     6001 atcatttcgg ctgtttcgga agcacgcatg cctagtttat tctctttctt accagccata
     6061 aaacctggag tgccacgctc tactaaaaag gctgtcattc catgcgaatc gagcaattct
     6121 ccagtacgta caattacaac agctaattca gcgctgatac catgtgtaat ccagcatttg
     6181 gtgccattga ttacccaata atcaccgtcc tgatgtgcta cacatttcat gcgcatcgca
     6241 tccgatcctg tgttaggttc tgttaagccc caagctccaa tccattcgcc gctagctaat
     6301 ttgggaagga attttttctt ttgatcttcg ttagcaaaca ttaaggtatg accagtacac
     6361 aaagaattat gtgcagccat agataagcca attgaaggat caattttgct taattcaatg
     6421 attgccgtta catattccgt atagcttaat ccagctccgc catattcttg aggaactaaa
     6481 acgcccatca atcctaattc acctaattgg cgaaaaattt ctcgagggaa atgttgcgtt
     6541 tcatcccatt ccatcatgtt aggacgaata tgcttatcac cgaaattacg aatcatttcg
     6601 gctatcattt tttgattttc gttcagctgg aaatccattt tttacgagct cgtttctttt
     6661 tatcgggcgc aaaaatagac gaaactccgc cctttacatc ttcaaattcc taaaaaatta
     6721 ttaaaaaatc aaagtgtcac atcgacaatt aaagaaatgc gaatgaacac acttaaatca
     6781 agctgataaa tgctgaatac tcaaaatcag atgggtagga tggaagtcag gctgaaaaaa
     6841 taccccaatc gaacgttatt tcttccgaat ccacttgaat gtttgggtgt tttgagcacc
     6901 ttttacacga acaaaataaa tccctgcatc taattgcaaa tctgttaatg ctacagttgg
     6961 ttggttggtt cctgattgga aatgcaccat tcttccttgc atatcaaata tttcaatctg
     7021 tttgattttt tcttgcaagc tgcgcacaaa caaatcagaa ccgggattag gatagattac
     7081 aaaattcggc tcttgagcag ttttattctc caaactactt gggaaaacac ccgaaaagaa
     7141 attgattcca cctgcataat tcccggtaac cgcatccaag aatccatcat cattcaaatc
     7201 tttcaaactc atgctggtgc gctctccatc gctagtacca aggagtaaag agtcttccaa
     7261 aacgaaagca ccactcaaat tcccatcaat tccactatac aagtgaaatc ttccacttaa
     7321 actgccacat agtaaatacg aaacgcccga tttacggaaa agggtaggag tagagaatcc
     7381 attgggatcg ccattttcag ctgtcgaaac ttgtccgaaa gtagtgttca cttcttgcaa
     7441 aatcatattg gttggtgtgc caaaattctt gtaatagtga atggttcctt cgcgacaacc
     7501 aatcaacata tccaacaatc catccgtatc cacatcaatt aactgtggtg ctgaataggt
     7561 tccaatgtcg atgccttgaa aattgggcga gctaaacaca aaattggcat catttcctgg
     7621 tgcagcttca ttgatataat agaaaattct tccatcggaa gttcctaaaa ccatatcctg
     7681 atcactgtct ccatccacat caccaaacga aggatgataa ttggttgtat tgccagttaa
     7741 ttgagccaat ccagcgaagt cggtatctac caatttaaat ttaggagatg tatcggtact
     7801 aatgtttttc aataaagcta gctgcgattt atacgttcct gcaggttgaa aataaccgta
     7861 gtttcctatg actatatcct gtaaaccatc gccattataa tcgaaaaacg ttgggatgca
     7921 accttcacca aagtcgagcg attcgtccaa taaaagatcc tttttcacaa acgtaaaaac
     7981 aggaatagca tccgttccat cgttatgata ataccaaata cacatatggt tctcggaacc
     8041 accttcattg ttcggactca caattaaatc gcgcactcca tcatgatcat aatcaagaaa
     8101 aaatgctgct gggaaaatgg gcaaattgat ataaggtgta cccgatggaa aaggcgtttg
     8161 agaactggtt atttgtccat aattcgcatt gccaccgtta atcaattgtg tcatagtgcg
     8221 atacgcaata tctcccaaga gcatgtcttt atcaccatca ccatcagcat catatgctaa
     8281 catggttgat cctgcatgac gaagcgcatt atttcttcct gtcggatggt cgcagctatc
     8341 gttaataacc acatcgttgg tcgaaaagct ttcggtaaat agtccccaat tatcgctttc
     8401 caatttgagc atgagtgtat cgcatcgacc gagttgttca acggccatat tgcggtgata
     8461 ttccacacaa gtgcctaaga ttccaaatac aagtaaatct tgatcaccat cgttatccat
     8521 gtcttccacc acaggtacat ccaccggaat acagaaaacc gctaatgttg ctggattaaa
     8581 ataagattgc aggctttcgg tatagagttc aaattgtaat cctgaagcgg agctgatgtt
     8641 tttatagact ttgaatccac tgttctcgta agtgaataaa tcggctttcc catcgcaatt
     8701 gaaatccttc acaatcagaa aatccttcac tttggggaac gaatacacta aactgtgctc
     8761 aaatcgataa tctggattcc ctggagttcc tgtatagata aatggtaaaa ttcgatctcc
     8821 cgttcgatca aacatcagta aatcctgaac gccatcattg ttaaggtcaa tagctccagc
     8881 ttgcatgtaa ttgaatcctc ctgtggtagg aaatttcagt tgaatcgaat caatcgaaaa
     8941 caccggaata tcatcgtttc gatccaaagc caatatagtt tgtgtgcttt gggccaaaat
     9001 acctgaacaa gtaaggaaaa tggagctaat cagtagggaa atgcgcatga acaaagatgt
     9061 taatttttcg catcccaaac atagtgaaag agcaatccta atcatcgaag tatctgacta
     9121 atttgatgat acattctacc cttgcgaatt ggtttcaatt gctaaaaggc ttgatattct
     9181 tgattttctg aataatttga gtgaattggt atttatggtt tgttgttcta tatttgccgt
     9241 ccgatttttt agaattattt aacaaaatcc aatgcaaaac aaaggtgcaa taagcttttt
     9301 cgcgatcatg cttgcgctcg tatgtctgtt cgagctttca tttacttgga aagcgagcag
     9361 tgtagaaagt gacgcaaaaa ttttcgctgc cggtgatccg aaaaaggaga aggcgtatct
     9421 cgactccatt gcgagtgaaa ctgtttacaa cgtgcttttc agaaagtaca cttaccgcga
     9481 atgtaaggcg aaggaactca atttgggtct cgacttgaaa ggtggtatga acgtaacttt
     9541 ggaagtagcc gtttctgacg tagttcgctc actttcaaac aacagtactg atccaacttt
     9601 cttgaaagcc atggaattgg ctaatgcaag acagaaaagt aacggtggag atttcatcga
     9661 cctttttgct gctgctgtta aagaaaccga tcctgctgct cgactttcct ctccagcatt
     9721 ctttggaaac aaagacttga aagatttggt taactccaaa atgactgatg aagaagtcat
     9781 taaggtgatt cgtaccaaag taaaatcttc ggttgataat gcttataccg ttatccgtaa
     9841 tcgtatagat aaattcggtg ttactcaacc taacattcaa cgtttagaag gttctgaccg
     9901 tattttggta gagcttccag gtgttaaaga acctgagcgt gttcgtaagc ttcttcaagg
     9961 tgctgctaaa ttagaattct gggaaaccat cgaaaataaa gatgctttcc aatacttaga
    10021 agcagctaat aagcgattaa aagatattac caatgcaggt acagctccag cagcagtgcc
    10081 agcggttact gatacaactg cagctgctac tccagtagat acaacagcaa cagttgcaaa
    10141 agatacaagt ctttcattag ctgaaaaatt agcaaaagct gatactactt ccgataaaga
    10201 tacttcggca gctgctcaac gtgctcgtgc tcaaaaagac aatcctttat atgttgttct
    10261 tagtccgaac atcaaccaaa ctaacgaagg aagtactttc cgtccaggtc cggttgtagg
    10321 tatctctctt actaaagata cttcacgtgt gaaccaaatt tttagcatgc ctcagattaa
    10381 agaagtcttc ccgaaagaca ttcgtttctt ctggagtgtg aaaccatttg atgagaaaga
    10441 aaatgctttc gaattagttg ctatcaaagt tcctcgtgat ggtaaacctc gcctaacagg
    10501 tgaatatgta acagatgctc gtttgagtta cggtcagtta aacaataaac cagaagtaac
    10561 tatggccatg aacggcgaag gtgctggtat ctggaaacgt atgactggtg agaatgttgg
    10621 gaaaagcgta gctattgtgt tggacaacta tgtatatagc tacccaacag tccaaggtga
    10681 aattgcaggt ggtaactcat ctatcactgg taacttcacc attaaagaag cggaagattt
    10741 atcgaacatc ctgaactcag gtaaaatgga tgctcctgct cgtatcgtgg aagaagctgt
    10801 tgtaggacca tctttgggtg ctgaagcaat caattcaggt ttactttcag ctcttgctgg
    10861 tttgattctc gtgttcttat tcatgggctt ctattacagt ggtggtggta ttgttgccga
    10921 tgttgccttg atcgcgaacg tattcttcct tatgggagtt atgacttcac ttggagcagt
    10981 attaacactt ccaggtatcg ctggtatcgt attaacaatt ggtatgtcgg tcgatgcgaa
    11041 cgtacttatc tacgagcgta ttcgagagga attagcttcc ggaaaagcat ataagcaagc
    11101 gattgctgat ggttacaaag gtgcttattc cgccatcatc gactcaaacg ttacaacctt
    11161 attaacgggt atcatccttt acattttcgg tactggtcca atccaagggt ttgcaactac
    11221 actcgttatc ggtatcttaa cttctttatt ctcggcaatt ttcatcactc gtttgtgctt
    11281 tgaattccta ggaaaacgta acaaagaagt gaaattctct attccaatga cagctaagtt
    11341 gttcagcgga agtaatttcg acttcatcaa ccaacgtaaa cgtttctaca ttgtatcact
    11401 tgcggttatt gtgattggtg ttggattcat ggtaactaaa ggatttggtt acggggtgga
    11461 ttttgaaggt ggtcgttcgt atgttgttcg tttcgacaag ccagtacaaa ccgatcaagc
    11521 tcgtgaagta ttgtacaaag cattccagaa gtctccagat gtgaaggttt acggaagctc
    11581 taaccagttg aaaattacaa caggttatat gttggaagac gaatcacttg ctgccgattc
    11641 aattgttgca agtaaaatgc tttctgagtt aactccgatc actggtgctg taccggttat
    11701 tcaatcatct caaaaagttg aaccaactgt agcgaacgac attaaagatt cagctttttt
    11761 atcagtaatc ttctcgttat tagtgttgtt cttatactta ctacttcgtt tccgtagatg
    11821 gcaatatggt attggtgcta ttgcagcact tgcatacgta gtaatattca tcctttcgat
    11881 tttctccatc tttaatcaca ttcttccatt ctcactcgat atcgatcaag cattcatcgc
    11941 tgcgatctta acagtagttg gttatgcgat taacgatacc gtagtaattt ttgaccgtat
    12001 tcgtgaatac ctcgctatgc ctaacagcaa acgtgaagat ttgtctacga tcattaataa
    12061 cgccttgaac agtacactat ctcgtactgc cgttaccggt ttaacaacca ttggagtatt
    12121 gattatcctc ttcatcttcg gtggtgaggt aatccgtgga tttgctttcg cgatgctaat
    12181 tggtgttatc gtaggtacat acacctcact cttcgttgcc tctccggtag ttgtcgattt
    12241 gactcgccgt gttgcaggta aatcggacga agaaaagaag taattttaca cactgaacct
    12301 gcaacaatcc cgttgtaggt tcagttttta gaccatgctg gcttcagtat accttttttt
    12361 tgatgtgagt ggcggagaga ttactgtaat cattctgttc attctcatct tttttggttc
    12421 taaaaagatt ccagaattag cccgtggttt aggtaaggga atgcgcgaat tccaagatgc
    12481 atcgaatagt attcgtcgcg aaatccaaac cgaggccaac aaaatcaaag acgaggtctc
    12541 caaagtacaa actcaggttc gcgaagaagt gaaactggat caactaacgg aagatgctcc
    12601 caatacaccc aagcctttgg attctgtagc acatgttgct acaccagaac ccaatccttc
    12661 tgtagttaca gacgaatcgg ctaccaatca aacaactaat ccggaagcag ctgaaatcaa
    12721 atcagaactt ccaacaacat cacaaatatg attcaaggac atattttcct cggagcactc
    12781 gggccaagcg aaatcattct tattggagca ggtattcttc tcctttttgg tggaaagaaa
    12841 attcctgaac taatgaaagg tcttggtcaa ggaatgcgtg aatttaaaga cgcaaaagac
    12901 ggtaaaaccg aacttcctga agccaataaa gaaaaacaaa cccagatcaa ttcctgatta
    12961 ctttttcttc ttaggaacac gtgctggtgt aggacg
//
LOCUS       DAOOQW010000049        12947 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_5955_length_12947_cov_5.489528, whole genome shotgun sequence.
ACCESSION   DAOOQW010000049 DAOOQW010000000
VERSION     DAOOQW010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 12947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12947
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5955_length_12947_cov_5.489528"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..914
                     /gene="lhgO"
                     /locus_tag="PK281_04610"
     CDS             <1..914
                     /gene="lhgO"
                     /locus_tag="PK281_04610"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF008726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HOY48659.1"
                     /translation="NQEQIPLLNNLYQRGIENGLTDIRKISGEELRKIEPHCAGIEAI
                     QVPYTGIIDYKEVSKKLAECILDSGGVIQTGTKVTGISLHDKGAKIQTSKGEYETKLV
                     INCAGLYSDKIAAMTHPEIDVRIIPFRGEYYKLRDDRRSLVKTLIYPVPDPNFPFLGV
                     HFTKMIDGGVEAGPNAVFAFKREGYTRTSFNASELWGSLSWPGFRKVATKYWKTGMGE
                     YYRSFSKAAFTRALQELIPEVQQSDLVPAGAGVRAQACDRSGGLLDDFAFLEKENVIH
                     VLNAPSPAATSSLSIGKTIAAMALKRF"
     gene            1099..4302
                     /locus_tag="PK281_04615"
     CDS             1099..4302
                     /locus_tag="PK281_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48660.1"
                     /translation="MKIYRLLFFFVHLVVFSFTVQAQGRREFIIYDGYLTSESGAISD
                     GASIRLLCNGRNEYLKFDPLTGKFQVRVGLNKECEISFSKPGSITTLISLNTFCPKER
                     QEEGFPDYELNVVLKPEIEKVPYTIKHDIVYDDDVYNFDFKENFARSTNRQLKEYKEL
                     TSVKKNSAEEQARLAEEKKKAEELKKQQLAEQKAAAERAKQLADSLASANNAADKAKR
                     EAEAKAAAAAEAAEKAKLENDLAARKAAEEKLRLEKELAEKARREAEEKARLDAIAKE
                     KADRDAREAAEKAKREADEKARLDAIAAEKAQKSAAEKARLEAEAEAKARREAEEKAR
                     LEALARDKAQKEADEKARREEIERARLAAETAKLAEKETNKRKRDSLENVMKQQQLAE
                     LKAQQELEARRKAEKEAADKARLEAEARQKAEAEAALKARKEAEAQAKAEAEARAAAE
                     KAALEKARLEAEARAKADAEAKAKAEKEAAEKARLEAEAKAKADAEAKAKAAAEAKAA
                     ESKAKAEAEAKAKAEAEAKAKADAEAKAAIEKARLEAEAKAKADAEAKAKAAADAKAA
                     EAKAKAEAEAKAKADAEAKAKAAAEAKAAEAKAKAEAEAKAKADAEAKAKTEAEAKAA
                     AEKARLEAEAKAKADAEAKAKAEADAKAAAEKARIEAAEKAKQDAIAKAAEEKAQKDA
                     ELKARAEAEAKAKAEAAAKAAEEKAAKAAAEKARLEAEAAEKAKLAAEAKAKAEAEAK
                     AKAEKDSLAKVQKEAEEKARLEAEAAVKAKKEAEEKARIEAELKAKADAEAQAKAKRE
                     AEEKAKVEREAAEKAKREADAKAKAEADAKAAAEKIAAEKARVEAEAKAKAEAEAQAK
                     ARAEAELKAKQEKEALEKAKREAEEKARQESIAKAAEEKAKREAEEKAKREAEEKARL
                     EAIAEAAKEKARKEAEEKATREAAEKARQEAIAKAAAEKARLEAEAKAKAEADEKARR
                     DAAAKIAADKAAKEAEEAAERAKPVDQRVRERIEKMLKTGVKEENVILRPYEAALQGY
                     ATKYYGYINFGDGKGNIELSKEEFLEYAKRFKVVQGQ"
     gene            complement(4349..5356)
                     /locus_tag="PK281_04620"
     CDS             complement(4349..5356)
                     /locus_tag="PK281_04620"
                     /inference="COORDINATES: protein motif:HMM:NF013116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HOY48661.1"
                     /translation="MELFLESHDLKEIEEAFKSGIPAGLTASVAFINRPASTDETALL
                     LKLAKMCSIIQIDALGEMAESIVHDAHRLLDLGISPETTVFRIPVSMEGIKACRILSK
                     EGLKVNIQSVFTLQQAYVAMQAGAAYISIPAGRLQDHGYDSQHVLEQCVSCAHTYGYQ
                     TRIMMTSVRSSDFVRSAIQMGVQAIALPWNIIRGLTENDYTKASNQQFFENTRLMTVQ
                     VKDALRGINPVVYLTDNILDAVVQMSQGGLGAVAVLDENQKVKGVFTDGDLRRLLQNG
                     GKDILQMKMGEMQFKSPISIESGAYLNDAARILKEKRIDNLLVIDNGKLIGMLDIQDL
                     S"
     gene            complement(5422..7155)
                     /locus_tag="PK281_04625"
     CDS             complement(5422..7155)
                     /locus_tag="PK281_04625"
                     /inference="COORDINATES: protein motif:HMM:NF036516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HOY48662.1"
                     /translation="MHDLHYQIGFSNPATQLIEICLTVRNNTESKVQIQLPSWRPGRY
                     ELGNFAKNIRSFSIEKFSGEVLNFQKIKKDLWEVETPNIAEFKVIYTYYACELNAGSS
                     YLDAEQLYVNPVNCCVYIPNRMNEACVLEIKVPGQYQIAIDLPAATEKNQFDCTNFDR
                     LADAPFIASAKLQHQIISTSNHHFHLWFMGDVKPDWERLNTDFKRFCDVQVDMMGDLP
                     ASDYHFYFQILPTAFYHGVEHTFSTVCALGPGTKIFKTDFYDELLGVSSHELFHAWNV
                     KTIRPSEMLPYDFTTENYSRMGWVYEGITTWYGDQFLLRSGVFDSKRYLKTLNDKLNR
                     HFSSYGRFNQAVAEASFDTWLDGYVPGIPHRKTSIYVEGSLVTCMMDFILREKSNDTV
                     TVDDLMRRLYADAIKGMAYSKERILQHLQELSGISFDSFYESYIEGKSDFEPQLEQGF
                     ARIGMKLEKSFLLSEAEHRFGIKLQETAGQLSITMLAPGSPAEKAGLMAGDVLLACNG
                     RKIAQNWSLLTDAQKSIQLHVFSKERLVQVDLAGDDLKWFVSRTVSMNPDASPAQRQA
                     YQKWAHQDFPQ"
     gene            complement(7158..8786)
                     /locus_tag="PK281_04630"
     CDS             complement(7158..8786)
                     /locus_tag="PK281_04630"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOY48663.1"
                     /translation="MRHKILSLILLATPLLNAQEIKKPKLVLGIVVDQMRFDYIYKYW
                     NKYGDGGFKRLVNGGYQCKNLQYNYVPTYTGPGHSSIYTGTTPERHGIISNDWFNRET
                     NRSIYCSEDPSVKTIGSKTEKVGQMSPRNLLSTTVGDELRVASKQGSRVYGIALKDRA
                     AILPAGHLANAAYWFDGATGNFVSSSFYCQELPKWLVEFNKKQKALAYLSQPWTTFLP
                     INQYTESLPDQTPYESPFKGTTSVTFPYNLKQLMATNDGQNLIRATPFGNSLTKDLAL
                     ELIYQEKLGKGDFTDMLCVSFSSTDYIGHYFGTDAVETEDVYLRLDADLKLMLDSIEA
                     YVGKENLIVFLTADHGGATVPAYLMDLRAPGGYMNYGPVESELKLWMKTQTGIDSNVI
                     GIFNDQVYLNEYDLKNNGNNISEIEWQVADWLLKVKGIHSTITASDLRRNEFTSGSNH
                     LIQKGFHPKRSGNVMITMEPNWMEYHKTGTTHGTAYNYDTRVPMLWYGWKIQPGETPL
                     PYYIDDIAPTVSWLLNIPFPNATSGNPIPLPLSR"
     gene            complement(8887..9756)
                     /locus_tag="PK281_04635"
     CDS             complement(8887..9756)
                     /locus_tag="PK281_04635"
                     /inference="COORDINATES: protein motif:HMM:NF022588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HOY48664.1"
                     /translation="MKKLFLFLFSISAVGSVSAQQIDTPRPSPLSTVTQKVGLTEFTI
                     TYSRPSAKGRKVFGDVVAFDKLWRTGANMATILKSSDEFSVAGTKIPAGEYSLFCIPG
                     STEWTVILNKTAKLSGTNGYKESEDAIRFKAKPVAITPAVETFSIGFNNLRDNAANIE
                     LSWENTRVSFEVQVEYDARVMKQIDDVMAGPTAGSYNSAASYYFNNGKDLNKALEYVN
                     KALEKGGEKYWILSLKAQIQAGLNDFKGAVETANKAKTMAQADEDDAYTKMNTDRINE
                     WTPKIQPAKKGKK"
     gene            complement(9763..11376)
                     /locus_tag="PK281_04640"
     CDS             complement(9763..11376)
                     /locus_tag="PK281_04640"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY48665.1"
                     /translation="MNLRNLLFCILFPLCASAQIDSFHVEIEPFSIADMPASHSGAIA
                     TYNGRWIILGGRINGLHGFQSPFAFPNNGKNESVWIVDPISGQKWSASLTGLPVNLYE
                     PLTSSNIPFYQDDNRLYMVGGYGWSDVAGDFRTFPTLTAIDLPCLHDAVVNGTSVETC
                     FRQITDSVLAISGSHLKKLNDRYYLIFGHRFDGIYAVNNGNNAFYHQTYSNEVRSFQI
                     QDDGVNLAITDYTALRDTVNFHRRDYNLVPQIFSDGEAGFTAFSGVFQYSVNLPYLNT
                     IDVRESSATVVPNFDQHLSQYHSAVMPVYDSTGNVMHSYFFGGMSRFTIDSLTQELVD
                     DTLVPFVKTISRISRFADGSMVEEQLPISFPEFLGSNMEFVPAPGVPLKQDAILNVNK
                     MQEDRQLAGYLVGGIWSPMANISSIDPSMSSANAQVYAVYIVRSSNDTGIVTQTLEAN
                     LPLAAFSCAPNPAIGSTQINLKLHQAGQVQLAAYTRKGELVEIISNQFMQAGEHKINW
                     SVSSLSSGTYILRARMNGVTKTVSIIINP"
     gene            complement(11376..>12947)
                     /locus_tag="PK281_04645"
     CDS             complement(11376..>12947)
                     /locus_tag="PK281_04645"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HOY48666.1"
                     /translation="ATRLGKMLRIDVSGATGFSIPPTNPFLTPGDNILDDIWALGIRN
                     PWRFSFDRLTGDLWIGDVGQDVYEEVDFIQAPDTGGQNYGWKCREGLHAYSGASCTVP
                     NRTDPVFEYAHGSTNGCSITGGYVDRGSLYASLNGRYFVTDYCSGRIWSLIPNGSGLA
                     TSTDHGVYVTNQYTTFGEDSYGEVYLAQQNGRISRLTTPDGSPMANINADGELAICPG
                     TTRTLSTGLNPLLSYQWYKDDVAISGATNNQYAATEAGTYKVDVTRVGAPTATSETLT
                     ITVFSVPELTASSAFSELCEDAAFPITLTGSPSGGTFSGTGVVDGNFDPFQLGAGTYT
                     IAYNYTTSDGCDAIPAEFDITLNPLPQVTLSIATTNFCIDSAIETLDVTPEGGQLNGN
                     GITNNSFDPAIAGLGEHVISYTYADANSCAGTASVTLNVESCLSVEDALKLGIQIYPN
                     PFKGKFQVSFDDNQSAWNLIEVFSITGQRCYSKPIQATGKQTIEIDLSAQPAGVYLLR
                     IQGETHQEVIKLIQE"
BASE COUNT         4156 a         2588 c         2762 g         3441 t
ORIGIN      
        1 cgaatcaaga acagattcca ttgttgaata atttatatca acgaggaatt gaaaatggct
       61 tgaccgatat acgcaagatt tctggtgaag aacttaggaa aattgaacct cattgtgctg
      121 gtattgaagc cattcaagtt ccatatactg gaattattga ctataaagaa gtttcaaaaa
      181 aattggctga atgtattcta gactcaggtg gtgtaattca aacgggtact aaagtcactg
      241 gaattagctt acacgacaaa ggagcaaaga ttcaaacgag caaaggtgaa tacgaaacaa
      301 agctggtaat taactgtgcc ggtttgtatt ccgataaaat tgctgctatg acacatcccg
      361 aaattgatgt gcgtattatc ccatttcgag gggagtatta taagcttcga gatgatcgtc
      421 gttctttagt gaaaaccttg atttacccag tgccagatcc taatttccca tttttgggtg
      481 ttcattttac caaaatgatt gatggaggag ttgaagcagg gcctaatgct gtatttgctt
      541 ttaaacgtga aggttatact cggacttcat ttaatgctag cgaattatgg ggttctttga
      601 gttggccggg atttagaaaa gtggcaacaa aatattggaa aactggaatg ggcgaatact
      661 atcggtcatt tagcaaagct gcttttacac gtgctttgca agagcttata ccagaagtgc
      721 aacaaagtga tttagtgcct gccggagctg gcgttcgggc tcaagcatgt gatcgttctg
      781 gcggcttact ggatgatttt gcgtttctgg aaaaggaaaa tgttattcac gtattaaatg
      841 cgccttcgcc tgctgctact tcatctttat caattggcaa aacaatcgca gctatggcat
      901 taaaacgatt ttagtcgcat acgattcaga attgaatacc gttatgaatg ttttagcaat
      961 tttaatgttc cattgttgat attttgaata aatttctacc agtaaaacat gtggaaatca
     1021 gccacagttg ttagttttgc aggtataatc caatcaagta ccctcttttg cccttttcaa
     1081 agggaatcaa agattgaaat gaaaatctac cgtttactat tcttttttgt ccatttagtt
     1141 gttttctctt tcactgttca agcgcagggc agaagagaat ttattatcta tgatggttac
     1201 cttacttccg aaagcggagc tatttctgac ggcgctagca ttcgattgtt atgcaatgga
     1261 agaaatgaat acctcaagtt tgatccactc acagggaagt ttcaagttcg tgttggctta
     1321 aacaaagaat gtgagataag cttttcaaag cctggaagta ttacaactct gatttcttta
     1381 aatacttttt gtccgaaaga gcgccaggaa gagggttttc cagattatga attaaatgtg
     1441 gtcttaaaac ctgagattga aaaagttcct tataccatca agcacgatat cgtatacgat
     1501 gatgatgtat ataatttcga tttcaaagag aactttgcac gttcgaccaa tcgtcagtta
     1561 aaagaataca aggaattaac cagtgttaag aagaattcag ctgaagagca agctcgttta
     1621 gctgaagaga aaaagaaagc agaagaactt aaaaagcaac agttagcaga acagaaagct
     1681 gcggcagaac gtgctaaaca attagctgat tcattagctt ctgcaaataa tgctgccgat
     1741 aaagctaaac gtgaagcgga agccaaagct gctgctgcag cggaagcagc tgaaaaagcg
     1801 aaactagaaa acgatttagc tgctcgtaaa gctgctgaag aaaaattaag attggagaag
     1861 gagttagctg aaaaggctag acgtgaagca gaagaaaagg ctcgtttaga tgcaatagct
     1921 aaagaaaagg cagatcgcga tgctcgagaa gcagctgaaa aagctaaacg cgaagcggat
     1981 gaaaaagcta gattagatgc tatagcagca gaaaaagcac agaaatctgc tgcggagaaa
     2041 gctaggctag aagctgaagc agaagcgaaa gctcgtcgcg aagcggaaga aaaggctcgt
     2101 ttagaagctt tagcacgcga taaagcacag aaggaagctg acgaaaaagc tcgtcgtgag
     2161 gaaattgaac gagctcgatt agctgctgaa acagctaaat tagctgagaa ggaaaccaat
     2221 aaacgtaagc gcgattcact cgaaaatgtg atgaagcaac agcaattggc tgaacttaaa
     2281 gctcaacaag aattagaagc tagacgcaag gcagaaaaag aagcagctga taaagctcga
     2341 ttagaagccg aagctcgtca gaaagcagaa gccgaagctg cattaaaagc acgtaaggaa
     2401 gcggaagcac aagcaaaagc tgaagcagaa gctagagctg ctgcagagaa agctgcatta
     2461 gaaaaagcac gtttggaggc ggaagccaga gcaaaagctg atgcagaagc aaaagcaaaa
     2521 gcagaaaaag aagctgcaga aaaagcacgt ttagaagcag aagctaaagc aaaagccgat
     2581 gcggaagcca aagccaaggc agcagctgaa gccaaagctg ctgaatccaa agcaaaagcg
     2641 gaggcagaag cgaaagcaaa agcagaagca gaagcgaaag caaaagctga cgcagaagcg
     2701 aaagcagcta ttgaaaaagc tcgtttagaa gccgaagcca aagcaaaagc agatgcggaa
     2761 gcgaaagcta aagccgctgc agatgcaaaa gctgccgaag caaaagcaaa agccgaagcc
     2821 gaagccaaag caaaagcaga tgctgaagct aaagcaaaag ccgcagcaga agcgaaggcc
     2881 gccgaagcaa aggcaaaagc agaagcggaa gccaaagcga aagcagatgc tgaagccaaa
     2941 gcgaaaaccg aagccgaagc caaagctgca gccgagaaag ctcgtttaga agcggaagca
     3001 aaagcaaaag ctgatgctga agcaaaagcc aaagccgaag ctgatgccaa agcagctgca
     3061 gagaaagctc gtatagaagc tgccgagaaa gctaaacaag atgcgattgc aaaagcagca
     3121 gaagagaaag ctcaaaaaga cgcggaatta aaagctcgtg ctgaggctga agcaaaagca
     3181 aaagcagaag ctgccgccaa agctgctgaa gaaaaagccg ccaaagccgc agccgaaaaa
     3241 gctcgtttag aagctgaagc tgcagagaaa gctaaactag cagccgaagc aaaagctaaa
     3301 gctgaagccg aagcaaaagc caaagccgaa aaagatagtc tagctaaagt tcaaaaagag
     3361 gcagaagaaa aagctcgatt ggaagccgaa gcagcggtta aagcaaagaa agaagctgaa
     3421 gagaaagcac gtattgaagc tgaattgaaa gcgaaagccg atgctgaagc tcaagcaaaa
     3481 gcaaaacgtg aagctgaaga aaaagctaaa gttgaacgtg aagcagccga aaaagctaaa
     3541 cgcgaagcag atgctaaagc aaaagcagaa gcagatgcta aagctgccgc ggaaaagatt
     3601 gctgctgaaa aagctcgtgt agaagccgaa gcgaaagcga aagccgaagc cgaagctcaa
     3661 gctaaagcac gtgcagaagc tgagttgaaa gctaagcagg aaaaagaggc acttgagaaa
     3721 gcaaaacgtg aggcggaaga aaaagcacgt caggaatcta tagctaaagc tgcagaggaa
     3781 aaagcaaaac gtgaagcaga ggaaaaagct aagcgtgaag ccgaagagaa agctcgttta
     3841 gaagcaattg ctgaagcagc taaagagaaa gcccgcaaag aagcggagga gaaagctaca
     3901 cgtgaagcag ccgaaaaagc acgtcaagaa gccattgcaa aagctgctgc tgaaaaagct
     3961 cgtttagagg ctgaagccaa agccaaagct gaagctgacg aaaaagcaag aagagatgct
     4021 gctgctaaaa tagctgctga taaagctgct aaagaggcag aagaagcagc tgaacgcgca
     4081 aaacctgttg atcaacgtgt tcgtgaacgt attgagaaaa tgctgaaaac tggggtgaaa
     4141 gaggaaaatg ttatccttcg tccttatgaa gcagctcttc aaggatacgc caccaaatac
     4201 tatggataca tcaactttgg tgatggtaaa gggaatatcg aacttagcaa agaggagttc
     4261 ctcgaatacg ctaaacgttt caaagtagtt caaggacagt aatccttcaa ctaatctact
     4321 ttacatgcta tacagtgctg tatgttattt aggacaagtc ctgaatatcg agcatgccga
     4381 ttagcttgcc gttatctatc accaatagat tatcgatgcg tttttccttc aatattctag
     4441 ctgcatcatt cagatatgct cccgattcaa tactaatcgg acttttgaat tgcatttcac
     4501 ccattttcat ctgaaggatg tctttacctc cattttgaag taaacgacgt aaatccccat
     4561 ccgtaaatac tccttttacc ttttgattct catctaaaac agccacagct cctaaacctc
     4621 cttggctcat ctgcaccact gcatcaagaa tgttatcggt taaataaacg accggattaa
     4681 taccacgcaa agcatctttc acctgaacag tcatcaatcg tgtgttctcg aaaaactgct
     4741 gattgctagc tttcgtgtaa tcattttcgg tgagtcctcg aatgatattc cacggtaatg
     4801 caatggcttg aactcccatc tgaatggcac ttctaacaaa atctgaagac cttaccgaag
     4861 tcatcatgat gcgtgtttga tacccatacg tatgagcaca acttacacat tgttccaaca
     4921 catgttgcga atcatatcca tgatcttgta aacgacctgc tggaatactg atatatgctg
     4981 caccagcttg cattgctaca tatgcttgtt gtagcgtgaa aaccgattgg atattcactt
     5041 tcaatccttc tttactcaag attcgacaag ccttaattcc ttccatcgat actggaattc
     5101 gaaacacagt tgtctcagga gaaataccca aatcaagcaa acggtgagca tcatgtacaa
     5161 tcgattctgc catttcaccc aaagcatcaa tctgaatgat tgaacacatt ttcgccagct
     5221 taagcaataa ggctgtttca tcagtcgaag caggacggtt tataaatgct accgaggcag
     5281 taagtccggc aggaataccc gatttaaagg cttcttcgat ttctttcaga tcatgtgatt
     5341 cgagaaatag ctccatggat gtgttcaata aataggatgc aaaggaaaca ggaaaatttc
     5401 agattatacg atttcgcccc atcattgagg aaaatcctgg tgagcccact tttgataggc
     5461 ttgtcgttga gctgggcttg catctggatt catactaact gtgcgactta caaaccattt
     5521 caaatcatca cccgctaaat caacttgcac caaacgttct ttagaaaaaa catgaagctg
     5581 aatggatttt tgagcatccg tcaacaaaga ccagttttga gcaatttttc ttccgttgca
     5641 agcaagtaaa acatcacccg ccattaaacc agctttttct gcaggtgaac ctggagcaag
     5701 catggtaata ctgagttgtc cagctgtttc ttgaagtttg ataccaaaac gatgttcagc
     5761 ttcagataaa agaaaagact tttcgagttt catgcctatt cgagcaaaac cttgttccag
     5821 ttgaggttca aaatcacttt ttccttcaat ataggattca taaaatgagt cgaatgaaat
     5881 acccgaaagc tcctgtaaat gctgaagaat acgttcttta gaatacgcca tcccttttat
     5941 tgcatctgca tacaagcgac gcattaaatc atctactgtt actgtatcat tagatttctc
     6001 tcgaaggata aaatccatca tacaggtaac taagcttcct tccacataaa tggaagtttt
     6061 gcggtgcgga atgcccggaa catatccgtc gagccaagta tcaaacgaag cttcagctac
     6121 agcttggttg aatcgcccgt aactgctaaa atgtcgattg agtttatcgt ttagtgtttt
     6181 gaggtaacgt ttcgaatcga aaactcctga acgcagcaaa aactggtcac cgtaccaagt
     6241 tgtgattcct tcgtaaaccc agcccattcg actgtaattt tcggtggtga aatcgtaggg
     6301 taacatctca cttggacgga tcgtttttac attccaagca tgaaacaatt cgtgtgagct
     6361 cacacctaaa agctcatcgt aaaaatctgt tttaaagatt ttagttcccg gtccaagagc
     6421 gcaaacagtg ctaaaagtat gttctactcc gtgataaaat gctgtaggaa gaatctggaa
     6481 atagaagtga taatctgaag caggtaaatc ccccatcata tccacctgaa catcacaaaa
     6541 acgtttgaaa tcggtgttta gtcgctccca atcgggtttc acatcaccca taaaccaaag
     6601 gtgaaaatga tgattacttg tcgaaattat ttgatgttga agtttagcag aagcaataaa
     6661 tggagcatct gccagtcgat cgaaattggt gcaatcaaat tgattttttt ccgtcgcagc
     6721 tggaaggtca atagctattt gatattgccc cggaaccttt atttccaata cacaagcttc
     6781 gttcattcga ttaggaatgt aaacgcagca attaactgga tttacataca attgctccgc
     6841 atcgaggtag gaagatcctg catttaattc gcatgcatag taggtatata taaccttaaa
     6901 ttcagcaatg ttaggtgttt caacttccca aagatctttc tttattttct ggaagttcag
     6961 cacttcgccg ctgaattttt cgatgctaaa actgcggata tttttagcaa agtttcctaa
     7021 ttcatatcgt cccgggcgcc aagatggtaa ctggatttga accttacttt cggtgttatt
     7081 cctaacagtc agacaaattt caatcagctg agttgccggg ttagaaaagc cgatctgata
     7141 atgtaaatcg tgcatgatta gcgagataaa ggaagtggaa ttggatttcc cgaagtggca
     7201 tttgggaaag gaatattgag caaccagctt accgttggag cgatatcgtc gatgtagtag
     7261 ggcagaggag tttctcctgg ttgaattttc caaccatacc aaagcatagg cacgcgtgtg
     7321 tcatagttgt aagcagttcc atgggttgtt cccgttttgt gatattccat ccagttgggt
     7381 tccatggtga tcatcacatt tccagaacgt ttcgggtgaa agcctttttg gattaagtga
     7441 ttactacccg aagtgaattc atttctgcgt aaatcgcttg ctgtgatggt gctatgaata
     7501 ccttttactt tcaataacca atctgcaact tgccattcga tttctgaaat gttgttacca
     7561 ttattcttta aatcatattc attcaaatac acttgatcat tgaaaatacc aatcacattg
     7621 ctgtcgattc ccgtttgagt tttcatccag agcttcaatt cactttcaac cggaccataa
     7681 ttcatgtatc cgccaggtgc acgtaaatcc attaaataag caggcactgt tgctccacca
     7741 tgatcggcag tgaggaaaac gattaggttt tcttttccca cataggcttc gatcgaatct
     7801 aacatcaatt tcaaatcagc atccaagcgc aaatacacat cttcggtttc tacagcgtct
     7861 gtaccgaagt aatggccaat gtaatcggtt gatgaaaaac tcacacaaag catatcggta
     7921 aaatcacctt tgcctaattt ttcttgatag atgagttcaa gtgctaaatc cttggtaagg
     7981 ctgtttccaa acggtgttgc acgaatcaaa ttctgtccat cattcgttgc cattaattgc
     8041 ttcaaattgt atggaaacgt aacggatgtt gtgcccttaa agggtgattc gtaaggtgtt
     8101 tgatcaggta aactttcggt gtattggttg atgggtaaga aagttgtcca aggttgagac
     8161 aagtatgcaa gtgctttctg ttttttattg aattcgacca accacttggg aagttcctga
     8221 cagtaaaaac tactacttac aaagttcccg gttgcaccat caaaccaata agctgcatta
     8281 gctaaatgtc cagctggaag gatagcagct cggtctttca aagcaattcc ataaacacgt
     8341 gaaccttgtt tgctagccac tcgtaactca tcgcccaccg tggtgcttaa tagatttcgt
     8401 ggcgacattt gccccacttt ttcggtttta ctaccaatcg tttttacact cggatcttcg
     8461 gagcaataaa tagaacggtt ggtttcgcgg ttgaaccaat cgttggagat gattccgtga
     8521 cgttcgggcg ttgtgcctgt gtagatggat gaatgccccg gaccagtata agttgggaca
     8581 taattgtact gcagattttt acactgatag ccaccattca ccagtcgttt aaaaccacca
     8641 tcgccgtatt tattccagta tttatagatg taatcgaaac gcatttggtc aacgactata
     8701 ccgagtacca atttgggttt cttaatttcc tgtgcgttca ataaaggagt tgctagcagg
     8761 attagactga gaattttatg gcgcataacg aaaatcaatg aggtaaaagt aaggaaagcc
     8821 gttggcctga atggctgtaa acaaaaaatc ccggcagaac cgggatttta ctggaagcat
     8881 tgctgcttat ttcttgcctt ttttagcagg ctgaatcttt ggagtccatt cgttgatgcg
     8941 gtctgtattc attttggtat acgcatcatc ttcatcggct tgcgccatag ttttagcttt
     9001 atttgccgtt tcaacagctc ctttgaaatc gttcaaacca gcttggattt gagcttttaa
     9061 acttaaaatc caatatttct ctccgccttt ttcgagtgct ttgtttacat actcaagtgc
     9121 tttgttgagg tctttaccat tgttaaaata gtaagaagcc gcgctattgt atgaacctgc
     9181 cgtaggacca gccatcacgt catcaatctg tttcataaca cgagcatcgt attccacttg
     9241 cacttcaaat gaaacgcgtg tgttttccca gctaagttca atgtttgcgg cattgtcgcg
     9301 caaattgttg aatccaatag agaacgtttc aacagcaggt gtgattgcta ctggtttagc
     9361 tttgaaacga attgcatctt ccgattcttt gtatccgttc gtacctgaaa gtttcgctgt
     9421 tttgtttaaa atgactgtcc attcagtact tccaggaatg cagaaaaggg aatattcacc
     9481 tgctggaatt ttagttccgg caacactaaa ttcatcactt gattttagaa tagtagccat
     9541 gttagctcca gttctccaga gtttatcaaa tgctaccaca tcaccaaata ctttacgtcc
     9601 ttttgcactt ggacgtgaat aagttatagt gaattctgtt aatccaactt tttgagttac
     9661 agtactaagc ggactaggtc ttggagtgtc aatttgttga gcagaaacgc tgcccactgc
     9721 agaaatacta aaaagaaata ggaaaagttt tttcatgaaa aattatgggt tgatgattat
     9781 ggatacagtt ttagttacgc cattcatacg agctcgtaaa atgtaagtgc ccgatgaaag
     9841 actggaaaca gaccagttga ttttatgttc acctgcttgc ataaattgat tggaaataat
     9901 ttctaccaat tcacctttac gcgtgtatgc agcaagttga acttgtccgg cttgatgaag
     9961 ttttaaattg atttgtgtgc ttccaattgc cggattaggt gcacaggaaa aagcagctaa
    10021 gggaagattc gcttctaaag tttgagtgac aattcccgta tcgtttgaag aacgcacaat
    10081 gtatacagca tacacttgtg cattagcaga actcatactt ggatcgatac tactaatatt
    10141 ggccatcgga ctccaaatgc cacccacgag ataaccagct aactgacggt cttcctgcat
    10201 tttattgaca ttcaaaatgg catcctgttt taaaggaaca cccggtgctg gaacaaattc
    10261 catattagaa cctaagaact cagggaagct aatgggtaat tgttcttcaa ccatagaacc
    10321 atcagcaaaa cgactaattc gactgatcgt ttttacaaat ggaacgagcg tatcatctac
    10381 caattcttgt gttaagctat caatcgtaaa ccgactcatt ccaccaaaga aatagctgtg
    10441 catgacattc ccggttgagt cgtaaacggg cataacagca ctgtgatact gacttaggtg
    10501 ttgatcgaaa ttcggaacaa cagtggcaga gctttctcga acatcaatgg tgttgaggta
    10561 aggcaagttg accgaatatt gaaacactcc agagaaagct gtgaatccgg cttcgccatc
    10621 agaaaaaatc tgaggaacta agttgtaatc tcgacgatgg aagttcacgg tatcacgcaa
    10681 agcagtgtaa tctgtaatgg cgagattaac tccatcatct tggatttgaa aggaacgaac
    10741 ttcgttgcta taggtttgat gatagaatgc gttattccca ttatttacag cataaattcc
    10801 atcaaaacgg tgaccgaata ttaggtagta gcggtcgttt agctttttca aatgtgagcc
    10861 actaattgct aatactgaat cggtaatttg acggaagcaa gtttcaaccg aggttccgtt
    10921 tacaactgca tcatgtaagc atggtaggtc gatggccgtt aaagttggaa aagttctgaa
    10981 atccccagct acatcgctcc atccatatcc tccaaccata tacaagcgat tatcatcctg
    11041 ataaaatgga atattggaag aagtcaaagg ctcatatagg ttgacaggca atccggttag
    11101 agaagccgac catttttgtc ctgaaatagg atcaacaatc caaaccgatt catttttccc
    11161 attattagga aaggcaaaag gtgactgaaa cccatgtagc ccgttgattc gtccacctaa
    11221 aataatccaa cgtccattgt atgtagcaat agcacctgaa tgggatgctg gcatatcagc
    11281 aatcgagaat ggttcaattt caacgtgaaa cgaatcaatc tgagccgaag cacaaagtgg
    11341 aaagaggatg caaaagagca gatttctcag gttcattatt cctgaatgag tttaattact
    11401 tcctgatgag tttcaccttg aatgcgaagc aagtaaacac ctgcaggttg agcgctaaga
    11461 tcaatttcga ttgtttgttt tcctgttgct tgaattggtt tagaatagca acgttgtccg
    11521 gtgatggaga aaacttcaat caaattccaa gctgattgat tgtcatcaaa gctaacttga
    11581 aatttacctt taaatggatt cggataaatc tgaattccga gtttcaatgc atcttcaaca
    11641 cttaaacatg attccacatt aagtgtaaca gaagcagttc cagcacaaga attcgcatca
    11701 gcatacgtat aactaattac atgttctcct aaacctgcta ttgctggatc gaatgagttg
    11761 ttagttattc cattaccatt taattgtccg ccttctggag tcacatcaag agtttcaatc
    11821 gcagaatcaa tacaaaagtt tgtagttgca atgctgagtg taacttgtgg aagcggatta
    11881 agtgtaatgt caaattctgc aggaatagca tcacatccat cgctagtcgt gtaattatat
    11941 gcaatagtat acgttccagc acctaattgg aatggatcaa agtttccgtc tacaacgcct
    12001 gttcctgaga aagtaccacc acttggacta ccagttagag taatagggaa agcggcatct
    12061 tcacatagct cagaaaaggc agaagaagca gttaattctg gaactgaaaa tacagtgatt
    12121 gttaaagttt ctgaagttgc agttggagca cctacgcgtg taacatctac tttgtatgta
    12181 cctgcttcgg tagctgcata ttggttgtta gttgcgcccg aaattgctac atcgtcttta
    12241 taccattgat agcttagaag tggattaagc cccgtgctca gggtacgagt tgttcctgga
    12301 caaattgcca attctccatc agcgttaata ttagccattg gactaccatc aggagtagtt
    12361 aagcgggaga ttcttccatt ttgttgagca aggtaaactt caccatacga atcttcgcca
    12421 aatgtggtat attgatttgt tacatatacg ccatggtcgg ttgatgttgc taaaccgcta
    12481 ccattaggaa ttagtgacca gattcttccg ctacaataat cggtaacgaa atatcgacca
    12541 ttcagagaag catacagact tcctcgatca acatatccgc cagttatcga acagccatta
    12601 gttgaaccat gagcatattc gaaaacagga tcagttctat ttggaaccgt acagctagca
    12661 cccgaataag catgtaagcc ttcgcgacat ttccaaccat aattttgtcc accagtatca
    12721 ggtgcttgaa taaaatcaac ttcttcgtat acatcctgtc caacatcgcc aatccaaaga
    12781 tcacccgtta aacgatcaaa gctaaaacgc caaggattgc gaatacctaa agcccaaata
    12841 tcatctagga tattatctcc tggggttaaa aatggattgg taggagggat actaaatcct
    12901 gttgctccac ttacatcgat acgtaacatt ttacctaaac gagtagc
//
LOCUS       DAOOQW010000050        12642 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_6148_length_12642_cov_4.965599, whole genome shotgun sequence.
ACCESSION   DAOOQW010000050 DAOOQW010000000
VERSION     DAOOQW010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 12642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12642
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6148_length_12642_cov_4.965599"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..869)
                     /locus_tag="PK281_04650"
     CDS             complement(<1..869)
                     /locus_tag="PK281_04650"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008990381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HOY48667.1"
                     /translation="MSRKIEIFDTTLRDGEQVPGCQLSTDEKVIIARELELLGVDVIE
                     AGFPISSPGDFLSVVELSKAVKVGVCALTRSRKEDIDVAGEALLRAKRGRIHTGIGSS
                     DIHIKHKFNTTREKVLEQAVWAVQYAKAKGVEVEFFAEDAGRADLAFLAQMVEAVIGA
                     GADVVNIPDTTGYCLPHLYGKRIQYLFDNVKNIDKATISVHCHNDLGLATANTIAGLI
                     AGAGQAEVTINGIGERAGNTSLEEVAMILRTHKDLGLHTNIKSEKLYALSSMVSDMMR
                     MPVQPNKAIVGANA"
     gene            complement(913..2013)
                     /gene="leuB"
                     /locus_tag="PK281_04655"
     CDS             complement(913..2013)
                     /gene="leuB"
                     /locus_tag="PK281_04655"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: protein motif:HMM:NF012407.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HOY48668.1"
                     /translation="MKKKIVILPGDGIGKEVTACGKKVLEQIADCFGHNFVFDYATIG
                     HEAIEATGTPLPDESLTKMRNCDAVLFGAVGHPKYDNDPSAKVRPEQGLLKMRKELGL
                     YANLRPIKLFDELLSASSIKPEILKGADILFFRELTGDVYFGERGRKDGNDTAYDTMI
                     YSRYEVERIAHKAFKAAQTRSKKLTSVDKANVLESSRLWREVVQEVAKQYPDVAVDHM
                     FVDSAAMKLIQSPTYFDVVLTGNLFGDILTDEASQIAGSMGMLASASIGDTVGLYEPI
                     HGSAHDITGKGIANPLASILSAALLLDISFGLKEESEAIIKAIQQTLKEGYRTHDIAD
                     STTPHEKILGTEVMGNVVCNQARILVNCQVPA"
     gene            complement(2111..2695)
                     /gene="leuD"
                     /locus_tag="PK281_04660"
     CDS             complement(2111..2695)
                     /gene="leuD"
                     /locus_tag="PK281_04660"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015754852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HOY48669.1"
                     /translation="MAKEKFITLKSTAVPLPIENIDTDQIIPARFLKATTREGFGENL
                     FRDWRYNSDNTPKDFVLNNTTYSGKILVAGKNFGCGSSREHAAWSIYDAGFRVVISSF
                     FADIFKGNALNNALLPLQVSDEFLKKIFTAIEKNPSTIITVNLEEQKVVLEDGSSESF
                     EINSYKRTCMMNGYDDIDYLLSLRDEIKHFELAH"
     gene            complement(2709..3047)
                     /locus_tag="PK281_04665"
     CDS             complement(2709..3047)
                     /locus_tag="PK281_04665"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOY48670.1"
                     /translation="MVFSLQDYLAKLPLPATEKWPHGVWDIEAFKNGTMSVILYHPHV
                     KDFQTTHEQDELYIVMSGSAELRIEGVVNSCKTGDVLFVPARKDHRFENFSEDFITWA
                     IFWGPKGGEK"
     gene            complement(3053..4462)
                     /gene="leuC"
                     /locus_tag="PK281_04670"
     CDS             complement(3053..4462)
                     /gene="leuC"
                     /locus_tag="PK281_04670"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013307076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HOY48671.1"
                     /translation="MASQRKTLFDKIWDAHIVKNSEGYPDAIYIDRHFIHEVTSPQAF
                     DGLRKRGIKVFNPSRTTATADHNVPTIDQHLPIKEALSRHQVETLRKNCPEFGIELYD
                     LGHPYQGIVHIIGPELGLTLPGMTIVCGDSHTSTHGAFGNIAFGIGTSEVEQVLATQC
                     ILQYRPKTMKIEINGKLGKGVLAKDIILYVIAKISASGATGYFVEYAGEAIRDLSMEA
                     RMTICNMSIEMGARGGLIAPDQTTFDYIKGRKFAPQGEKYDRLVEQWRQLKTDDGAEY
                     DLVLKYDAADIGPMITYGTNPGMGMRVTDRIPTLEELKDISEQTSFSKSLDYMGLEPG
                     SALLGKSIDYVFIGSCTNARIEDLRLVASLVAGKKKADGVEVWVVPGSKQVEDQARKE
                     GLDKIFEAAGFQLRQPGCSACLGMNEDKIPAGKYCISTSNRNFEGRQGPKSRTFLASP
                     LSAAAAAITGKVTDVRELV"
     gene            complement(4789..5562)
                     /locus_tag="PK281_04675"
     CDS             complement(4789..5562)
                     /locus_tag="PK281_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008610659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HOY48672.1"
                     /translation="MAQGLLIDMDGVVYGGDTMIPGADVFIARLLKENVPFMFMTNNS
                     QRTRLEAVRKLTKLGIHVTEKHVYTSAMATAKFLADQTQKCTAYVLGEGGLITSLHEN
                     GITLVDNDPDFVVLGEGRNFTLEMVQRAVDMILAGAKFITTNRDPSPKMKGWNNLGIA
                     ATTAMIEEATGVHAFVVGKPGPVMMRAARKALGLETAETTVIGDTMVTDIQGGVQMGY
                     KTILVLSGITKVDDLKKYAFKPDLVVDSVNEIALPLSWW"
     gene            complement(5808..6269)
                     /gene="tnpA"
                     /locus_tag="PK281_04680"
     CDS             complement(5808..6269)
                     /gene="tnpA"
                     /locus_tag="PK281_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014083778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HOY48673.1"
                     /translation="MSDTYTQLYIHLVFSVKGRNNFIQSIWEDELHKYITAVVQTDRH
                     KMLAINGMPDHIHIFLGLNPAFSISDLVKDIKRASNNWINDKGFVKGRFEWQSGYGAF
                     SYSRSQIDSVCKYIHNQKIHHARFSFKSEYVELLKLFDVSFKADYLFEFYD"
     gene            complement(6766..8019)
                     /gene="ilvA"
                     /locus_tag="PK281_04685"
     CDS             complement(6766..8019)
                     /gene="ilvA"
                     /locus_tag="PK281_04685"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009088991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HOY48674.1"
                     /translation="MASDTKAVNLDINAAAEVLKSVVLKTPLQFHRKLSEKFKAEIYL
                     KREDLQVVRSYKLRGAYNLIQSLTEEQRKRGVVCASAGNHAQGVAYSCRTLDIKGVIY
                     MPAITPKQKITQVRMFGGQNIEIVLIGDTFDECQTHALEFTTANNMVFIPPFDHLKVI
                     EGQGTVGKEIIDELSNIDYIFLPIGGGGLCAGVGQYFKVFSPQTKIVGVEPQGAPSMK
                     EALKAGKPVVLDSIQRFVDGASVKKVGDITFELCKDTLSDMLLVPEGKVSSTILQLYN
                     EDAIVAEPAGALSIAALDQYVDHLRGKKVVCVLSGGNNDIDRMQEIKERSLLYEGLKH
                     YFIVRFAQRPGALKEFVNDILGPNDDIVRFEFIQKNNKESGPALIGIEVKSKEDFHVL
                     IDRMNAHKLNYTLVNQNENLFEYLV"
     gene            complement(8049..8627)
                     /locus_tag="PK281_04690"
     CDS             complement(8049..8627)
                     /locus_tag="PK281_04690"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOY48675.1"
                     /translation="MTEEINQHFPKAYNRIDEATKLLGFTMASDVLTCSLLRTLASSK
                     PASKFLELGTGTGLSTAWILDGMDNDSTLTSIDFDAKFLEIAESFLGDDKRLSLVCTD
                     GADWVEANGDKKYDYIFADTWHGKYLMLDEVLAMLNRGGLYIIDDMMPQPNWPDGHHE
                     KAIKLVDLLEKRNDLVLTKQVWATGIIVAVKK"
     gene            complement(8728..9798)
                     /gene="ilvC"
                     /locus_tag="PK281_04695"
     CDS             complement(8728..9798)
                     /gene="ilvC"
                     /locus_tag="PK281_04695"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016199816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HOY48676.1"
                     /translation="MAQINFGGTVEEVVTREEFPMEKALDVMKNETIAIIGYGVQGPA
                     QALNLRDNGFNVIVGQRKGSKTWDKAVQHGWVPGETLFEIEEAVKKGTVVQFLLSDAG
                     QIALWPTIKPYFTPGKTLYFSHGFGITFKEQTGIIPSKDIDVILAAPKGSGTSVRRLF
                     LQGKGINASYAVFQDATGNAKTKAIALGIGVGAGYLFETDFKKEVYSDLTGERGTLMG
                     AIAGIFEAQYEVLRANGHSPSEAFNETVEELTQSLMPLVAENGMDWMFANCSTTAQRG
                     ALDWMKKFKAATLPVFQELYKSVATGEEARRTIDTCSKPDYREKLAEELKEVRDSELW
                     QAGAAVRSLRPEKNAVEASMIN"
     gene            complement(9843..10136)
                     /locus_tag="PK281_04700"
     CDS             complement(9843..10136)
                     /locus_tag="PK281_04700"
                     /inference="COORDINATES: protein motif:HMM:NF025087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOY48677.1"
                     /translation="MKQNFTLAIHSDNNFSVLNRIVNILNRRRVRIKKLMAHENENDF
                     TRGMALILVHTTPDLMEKVKHQLEKLIEVEQALYYEGSDVYIELSAKHTEVAA"
     gene            complement(10196..11890)
                     /gene="ilvB"
                     /locus_tag="PK281_04705"
     CDS             complement(10196..11890)
                     /gene="ilvB"
                     /locus_tag="PK281_04705"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HOY48678.1"
                     /translation="MISGSETLLQCLVAENVNTLFGYPGGAIMPVYDALYGYADKLTH
                     ILVRHEQGAVHAAQGFARVSGKVGVALATSGPGATNLVTGLADALIDSTPIVCITGQV
                     FAHLLGTDAFQEIDVINTTIPVTKWNIQVTEAKDIAPAIAKAFYIARTGRPGPVLVDI
                     TKNAQNEMIAEAEYVPCKNIRTYKPKPELNLSQVEEAAKLINAAKRPYILAGQGILLS
                     GATEALIAFSEKTGIPVASTLLGLGGFPTDHPNYVGYLGMHGNYGPNVNTNECDVLIA
                     VGMRFDDRVTGNVSKYAKQAKVVHIEIDRAEINKIIPATVAVHADAKEALTALITKCD
                     SNQYPEWIGSFHKMKDIEFEKVIKHEFNPTGELMMAEVIHHLSEMTNGEAIIVTDVGQ
                     HQMTTSRYYEYKNPRTNVTSGGAGTMGFALPAAIGAKLGMPVKQVIAVIGDGGYQMTV
                     QELGTIMQYKIPVKILVLNNNFLGMVRQWQQLFHEKRYSFTEMSNPNFVKLAEAYDIP
                     ARKVEKREDLKAALKEMLDAETSFFLEAVVGKEDNVFPMVPAGAGVAEVILEIPKK"
     gene            complement(11937..>12642)
                     /locus_tag="PK281_04710"
     CDS             complement(11937..>12642)
                     /locus_tag="PK281_04710"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009088999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HOY48679.1"
                     /translation="SVMKYLLEKGFIHGDCITVTGKTVKENLKDAKSLDFTTQDVIHP
                     LENPIKATGHLQILYGNLAEKGSVAKITGKEGEKFNGPARTFNSEFDLNAGISEGRVK
                     AGDVVVIRNVGPKGAPGMPEMLKPTSAIMGAGLGKSVALITDGRFSGGSHGFVIGHIT
                     PEAYDGGLIGLVEDNDPIEIDVRGRTINLKVDAATLAKRKAAWVQPKLRVKSGVLLKY
                     AKQVKTAADGCVTDEN"
BASE COUNT         3398 a         2972 c         2305 g         3967 t
ORIGIN      
        1 gcattcgcgc caacaattgc tttattcggt tgcacaggca tgcgcatcat atcagaaacc
       61 attgaactta atgcgtacaa tttctccgat ttaatattag tatggagacc caagtcttta
      121 tgggttctca agatcatagc aacttcttca agtgaagtgt ttccagcacg ttcaccaata
      181 ccgttaatgg taacttcagc ttgtcctgca ccagcaatta aaccagcaat ggtatttgcc
      241 gttgctaatc ccaagtcatt gtgacaatga acagagatcg ttgccttatc aatattcttt
      301 acattatcga ataaatattg aatgcgttta ccatataaat gtggtaagca ataccctgtt
      361 gtatccggaa tgtttacaac atctgcacct gcaccaataa cagcttccac catttgtgca
      421 aggaaagcca ggtcagcacg acccgcatct tccgcaaaaa attctacctc tacacctttt
      481 gccttcgcat attgaacagc ccacactgct tgctctaaaa ccttctcacg tgtggtgttg
      541 aatttgtgct tgatgtgaat atcagatgaa ccaataccgg tatgtattct accgcgcttt
      601 gcacgaagta gtgcttcacc agcaacatca atatcttctt tgcgggagcg agttaaggca
      661 catacgccaa cttttactgc tttcgaaagt tcgacaacag aaagaaaatc accaggactg
      721 gatatcggaa atccagcttc gataacatca acaccgagaa gctcaagctc gcgtgcaatg
      781 atgacctttt catcggttga caattgacag ccgggcactt gctcgccatc gcgaagcgta
      841 gtgtcgaaga tttcaatttt tctgctcatg gttaatttgt tatcggtgtt ctgtttgtag
      901 ttctgaattt atttatgctg gcacttgaca gttaaccagt atgcgcgcct gattgcatac
      961 tacattgccc atcacttctg ttcccaggat tttttcgtga ggagtagtag aatctgctat
     1021 atcatgtgtt ctgtatcctt cttttaatgt ttgttgaatg gctttgatta tagcttctga
     1081 ttcttctttc aatccgaaag aaatatcaag aagtaatgct gcagaaagga tcgaagccaa
     1141 tggatttgct attcctttac cggtaatgtc atgtgcactt ccgtggatgg gttcgtacaa
     1201 acctactgta tcaccaattg atgctgatgc caacattccc attgaaccag caatctgtga
     1261 tgcttcgtct gtaaggatat cgccaaaaag atttccggta agtactacat caaaatacgt
     1321 tggactttga atgagtttca tcgcagcaga atcaacaaac atgtgatcaa ctgctacatc
     1381 cggatattgc ttagcaacct cttgaacaac ttctctccac aagcgtgaac tttccaacac
     1441 attggcctta tcaaccgatg ttaatttctt gcttcttgtc tgtgcggctt taaaggcttt
     1501 gtgcgcaata cgttctactt cgtaacgtga atagatcatg gtatcgtaag cagtatcatt
     1561 accatcctta cgaccacgct caccaaaata cacatcgccc gttaactcac ggaaaaataa
     1621 aatatcagca cctttcagga tttctggttt aatgcttgag gctgaaagta gttcatcaaa
     1681 caattttatt ggacgaaggt tagcatacaa gcctaactcc tttctcatct tcaacaagcc
     1741 ttgttcagga cgaacttttg ccgatggatc gttatcgtac tttggatgac ctactgcacc
     1801 gaataaaaca gcatcacagt ttctcatttt cgtcaatgat tcatcaggaa gcggtgtacc
     1861 cgttgcttca atggcttcgt gcccgatagt agcgtaatcg aaaacaaaat tgtgcccgaa
     1921 gcaatcggca atttgttcta ataccttctt gccacaagcg gttacttctt ttccgattcc
     1981 gtctccaggc agtattacta ttttcttttt cattcaactt tagttcaagg ttccgagttt
     2041 aaagtttcgg gtttcgagtt tgaaatgcgt aaaccgaaaa gtctaaatac taatgtctaa
     2101 atactcaata ctagtgtgct aattcaaaat gcttgatctc atcgcgtaaa cttaataggt
     2161 agtcgatgtc gtcataccca ttcatcatac aggttctttt gtatgaattg atttcaaagg
     2221 attctgaact gccatcttcc aacactactt tctgttcttc aaggtttaca gtgatgatag
     2281 tcgatggatt cttttcaatt gctgtgaata tctttttcaa aaattcatcc gacacctgca
     2341 gcggaagcaa agcgttattc aatgcattac ctttaaaaat atccgcaaag aagctggaga
     2401 tcactacgcg gaatcctgca tcataaatag accatgctgc atgttcgcgg ctactgccac
     2461 atccaaagtt ttttcctgct accagaattt ttccggaata ggttgtattg ttcagtacaa
     2521 aatcttttgg tgtgttgtcg ctgttgtaac gccagtcacg aaaaagattt tcaccaaaac
     2581 cctcacgtgt tgtcgctttc aaaaagcgcg ctggaataat ctgatcggta tcaatatttt
     2641 caatcggcag tggaactgct gtgcttttta atgtaatgaa tttttctttt gccatgtctc
     2701 tctcaatatt atttttctcc gcccttcggg ccccagaaaa ttgcccaggt tataaagtcc
     2761 tctgaaaaat tttcaaaacg atgatctttt cttgctggaa caaacaacac atcacctgtc
     2821 ttacaagaat tcacaacacc ttcaattcgt aattcagcac ttccactcat cacgatatac
     2881 agctcatctt gttcatgtgt cgtttggaag tctttcacat gaggatgata taaaatcacc
     2941 gacatggtac cattcttaaa agcttcaata tcccaaacac cgtgcggcca tttctctgtt
     3001 gctggcagtg gtaattttgc gaggtagtct tgtaatgaaa ataccatgct cgttacacta
     3061 actctcttac gtctgttacc tttcctgtga tagctgccgc tgctgcactc aatggactcg
     3121 ccaaaaatgt tcttgacttt ggtccttgtc ttccttcaaa atttctgttc gatgtgctga
     3181 tgcaatattt acctgccgga attttatctt cattcattcc caagcatgct gagcatccgg
     3241 gttgacgaag ttgaaatcct gcagcttcaa aaatcttatc gagtccttct ttgcgtgcct
     3301 gatcttctac ttgttttgat ccaggcacaa cccaaacttc aacaccatca gctttcttct
     3361 taccggcaac aagcgatgca accaaacgca aatcttcaat gcgcgcattc gtgcagcttc
     3421 cgataaaaac ataatctatc gactttccta ataaagctga gccaggctca agccccatgt
     3481 agtcgagtga tttgctgaaa gatgtttgtt cggaaatatc ctttaactcc tcaagcgttg
     3541 gaatgcgatc tgttacacgc atgcccatac ctgggttggt tccataggta atcatcgggc
     3601 caatatccgc agcatcatac tttaatacta aatcgtattc agcaccatca tctgttttta
     3661 attgtctcca ttgttcaaca agacgatcat acttctctcc ttgaggtgca aactttcttc
     3721 ctttgatata atcaaatgtt gtttgatcag gtgcaatcaa tcctccgcgt gctcccattt
     3781 caatactcat gttgcaaatg gtcatgcgtg cctccattga caagtcgcga attgcttcgc
     3841 cagcatactc aacaaaatat ccggtagcac cgcttgctga aattttcgcg attacatata
     3901 agatgatgtc tttcgccaat acgccctttc caagtttacc attgatttca atcttcatgg
     3961 tcttgggacg atattgtaaa atacattgtg tcgccaatac ttgttctacc tcactggttc
     4021 caataccaaa cgcgatgtta ccgaaagcac cgtgcgttga tgtatggcta tcaccacaca
     4081 ctatcgtcat gcccggcaag gtcaatccca actcaggacc gatgatgtga acaatacctt
     4141 ggtatggatg accaagatca tataactcaa ttccaaactc cgggcaattc ttgcgaaggg
     4201 tttctacctg gtgacgtgac aacgcttctt taatcggaag atgctgatca atcgtaggta
     4261 cgttgtgatc ggctgtagcc gttgtacgtg atggattaaa aactttaatg ccacgtttac
     4321 gcaaaccatc aaacgcctgc ggactggtta cttcatgaat aaaatgacgg tcgatgtaaa
     4381 ttgcatccgg ataaccctca ctgtttttca caatgtgagc atcccagatt ttgtcaaata
     4441 atgttttccg ttgtgaggcc atgtttcttc ttaattcaac ttctcttttc aaatgtgtaa
     4501 caagtgttag tttgtacacg aaggttaaaa acgcaaccgc cccatccttc ccgatagcta
     4561 tcgggagtgg acggggcggt atacaaaggt ctgatgttat tctgtcatct catatgagat
     4621 gtactttaat caaaaaaata aaaaattaat aagagagttc ttgatgtgcg aagatgaaaa
     4681 aagttaaggc atgcatcaac gaattgttaa aaaattttta ccaaaactca caagtgtttg
     4741 gtagcgattt agcattgcaa acggtagatg cacgtaagaa gaaaaatgtt accaccacga
     4801 taatggcaat gcaatttcat ttactgaatc aaccaccaaa tcaggtttga acgcatattt
     4861 ttttaagtcg tcaactttcg taattccact taaaaccaag atcgttttat atcccatctg
     4921 cactcctcct tgtatatcgg tcaccattgt atcaccaatt actgtagtct ctgccgtttc
     4981 taacccaagt gcttttctgg cggcacgcat cattacggga ccaggctttc ctaccacaaa
     5041 tgcatgaaca cctgtggcct cctcaatcat ggcagtagtt gctgctattc ccaaattatt
     5101 ccatcctttc atctttggtg atggatcgcg atttgtagta atgaattttg ctcctgctaa
     5161 aatcatatca accgcgcgct gcaccatctc caaggtaaag tttcttcctt cacccagcac
     5221 aacaaaatcg gggtcgttat caaccaatgt tattccgttc tcatgaagac tggttattaa
     5281 tccgccttca cctaatacat acgctgtaca tttctgggtt tggtcggcta gaaatttagc
     5341 cgttgccatt gcgctggtgt aaacatgttt ttcggttaca tgtatgccaa gcttcgtaag
     5401 tttccgtacc gcttccaatc gggtacgttg actattattc gtcatgaaca tgaaggggac
     5461 attttccttt aacaaccggg caataaatac atccgctccg ggaatcatgg tgtcgccacc
     5521 atataccaca ccatccatgt caattaataa accttgtgcc atttctttta ttgatcttta
     5581 attcgttgga tcgaagatag gaattttaaa gatcaggcct tcacgatctt tgtgttatca
     5641 atcaaaatgt tttgccctgt attttttatt tcaatacaac agggggttaa aaaaggtgca
     5701 actagaatct aacaaacgaa aacatttacg ccagaggcgt aacctctttg tagaaaccgt
     5761 atgcgttagc gcatttgccc cggaggggca acctctttgt ccggctgtta atcgtaaaac
     5821 tcgaagaggt aatcggcttt gaatgaaaca tcaaacaact ttaacaactc cacatactcg
     5881 gatttgaaag aaaatctagc gtgatgaatc ttttgattat ggatatactt acatactgaa
     5941 tcaatctgcg atctgctata agaaaaggca ccatatccac tttgccattc aaatcttcct
     6001 ttcacaaatc ctttatcatt gatccaattg ttactcgccc tctttatatc cttcactaaa
     6061 tcagaaattg aaaatgcagg attcaaacca aggaagatgt gtatatgatc tggcatgcca
     6121 ttaatggcca acattttatg tctgtcggtt tgcacgacag cagtgatgta tttgtgtaat
     6181 tcatcctccc agattgattg aatgaaatta tttctgcctt ttacagaaaa tactaaatgg
     6241 atgtacaatt gtgtataggt gtcactcata attcgtgctg ctaacaggtt gccctccggg
     6301 gcaaattatc aaaacatgtt ttattccaca aagaggttgc cccgctgggg caaatgcatc
     6361 ttattataaa attaatacta ttagtcaaac tataaagttg tttctaccac gaacagagaa
     6421 atctaaacga atgtataatt ggtgtatttt atatgcacta ttcataatcc gtgctgctaa
     6481 caggttgccc ctccggggca aattattgaa acatacttta ttctacaacg aggttgcccc
     6541 gctggggcaa atgcatctta gcataaagtt taatctaaca cacctaattt gcaaactaac
     6601 ggcatcgtag tttcttgcgc cagagacgta acctctttgt agaaaaaaac gatttatttt
     6661 aaggctttgc cccagagggg caacctgttt gtgttcggca ttattggcac gactcaagat
     6721 tgcatcgccc gattgaatta actactgaag aaatgacaac tattcttaaa ccaaatattc
     6781 aaacaaattc tcgttctgat tcacgagtgt gtaattcaat ttgtgcgcat tcatccgatc
     6841 gatcaacacg tgaaaatctt ccttcgattt gacttcgata ccaatcaatg ccggaccgct
     6901 ctccttattg tttttctgaa tgaactcgaa acgcacgata tcatcattcg gaccgagaat
     6961 atcatttaca aattccttca acgctcctgg acgctgagca aagcgcacga tgaaataatg
     7021 ctttaatcct tcgtacaaca acgaacgctc ttttatttcc tgcatgcgat cgatgtcgtt
     7081 gttaccaccg cttaacacac atactacttt cttacctctt agatgatcca catattgatc
     7141 cagcgctgcg attgacaatg ctcctgctgg ctcggctaca atggcatctt cattgtaaag
     7201 ttgaagaata gttgaactca cctttccttc cggaacaaga agcatatccg acaatgtatc
     7261 cttgcacaat tcaaatgtga tatcaccaac cttttttaca gatgctccat caacaaaacg
     7321 ttgaatcgaa tccaacacta ctggcttgcc cgctttaagt gcttccttca ttgagggcgc
     7381 accttgcggt tcaacaccaa caatttttgt ttgaggtgag aatactttaa aatactgccc
     7441 tacaccagca cacaatcctc ctccaccaat tggcaagaag atgtaatcaa tgtttgagag
     7501 ttcatctatt atttctttgc caaccgtccc ttgtccttcg atcactttta aatgatcgaa
     7561 tggtggaatg aataccatgt tattcgctgt cgtaaattcc aaggcatgcg tctggcactc
     7621 gtcaaacgta tcgccaatca aaacgatctc gatgttttgt ccgccaaaca ttctcacttg
     7681 cgtaattttt tgtttggggg taatggcagg catgtagatt acacctttaa tatctaacgt
     7741 gcgacaagaa tacgcaacgc cttgcgcatg attacctgca ctcgcgcaca ctactccgcg
     7801 cttgcgttgt tcttctgtta agctttgaat taaattatag gcaccacgaa gtttgtacga
     7861 acgcaccact tgcaaatctt cgcgcttcaa ataaatctct gctttgaatt tttcagagag
     7921 tttacggtga aactgcaaag gtgttttcaa caccaccgat tttaaaactt cggctgctgc
     7981 gttgatgtcc agattaactg ctttcgtgtc ggatgccatt gttgtgttgg ctaaaatatt
     8041 attacttctt attttttaac agcaacgatg attcccgttg cccaaacttg ttttgtcaaa
     8101 actaaatcat ttcgtttctc taacagatca acgagcttga ttgctttttc atgatgacca
     8161 tccggccagt ttggctgtgg catcatatca tcaataatgt acagaccacc tctatttaac
     8221 atggcaagaa cctcatctaa catcaggtac ttaccatgcc acgtgtctgc aaagatgtaa
     8281 tcatactttt tatctccgtt cgcttcaacc caatctgcgc catcggtgca aacgagactt
     8341 aagcgtttgt catcccctaa aaacgattct gcgatctcta aaaactttgc atcaaaatca
     8401 attgatgtaa gtgtagagtc attatccata ccatccaaaa tccatgcagt cgataaacct
     8461 gttccggtgc cgagttcaag aaacttagaa gctggctttg agcttgctaa tgttctcaac
     8521 aacgaacacg tcagcacatc agacgccatg gtaaaaccca acaactttgt tgcctcatca
     8581 atacgattgt aggcctttgg aaagtgctgg tttatctctt cggtcatttg ccagtaatta
     8641 atttgtacac accttgttaa atgacttcag gtataagttt aaagtttcga gtttgaatta
     8701 actcgaaact ttaaactcga aactatttta gttaatcata ctcgcttcaa ctgcattctt
     8761 ctctggacga agtgaacgca cagcagcacc tgcttgccaa agttcgctgt cgcgtacttc
     8821 tttcaattct tcagcaagct tctcgcgata atctggtttg ctacacgtat cgatcgtacg
     8881 tcttgcttct tcgcctgtcg caacgctctt atataattcc tggaatacag gaagtgttgc
     8941 agctttgaat ttcttcatcc aatccaatgc tccacgctgc gctgttgttg aacagttagc
     9001 aaacatccag tccattccgt tttcagcaac aagtggcatc aaactttgtg taagttcttc
     9061 tactgtttcg ttgaaggcct cagatggtga gtgaccattc gcacgcaata cttcgtattg
     9121 tgcttcgaat atacctgcga tagcacccat caaagttcca cgttcacctg ttaagtcaga
     9181 gtacacttct tttttgaaat ctgtttcgaa caaataacct gcacccactc caatacctaa
     9241 agcaatagct tttgtttttg catttcctgt tgcatcctgg aacacagcat agcttgcgtt
     9301 gataccttta ccttgtaaga acaatctgcg aacagaagta cctgatcctt ttggagcagc
     9361 aagaattaca tcgatatctt tagatgggat aattccagtt tgctctttga aggtgatccc
     9421 aaatccgtga gagaaatata aagtcttacc cggtgtaaaa tatggtttga tcgttggcca
     9481 caatgcaatc tgaccagcat ctgaaagaag gaattgaaca acagtacctt tcttcaccgc
     9541 ttcttcaatt tcgaataacg tttcgccagg aacccaacca tgttgaacgg ctttgtccca
     9601 ggtcttggaa cctttgcgtt gtccaacgat tacattgaat ccgttgtcgc gcaagttcaa
     9661 tgcttgtgca ggaccttgaa caccatatcc gataattgca atcgtttcat tcttcattac
     9721 atccaatgct ttttccattg gaaactcttc gcgggttaca acttcctcta cggttccgcc
     9781 aaaattgatc tgtgccattt ttattgtgtt taattgttta cggtttgatt ttttaattct
     9841 tattacgctg ctacttcagt gtgttttgca ctcagctcga tatacacatc acttccttca
     9901 taatagagag cttgttctac ttcaattagt ttttcaagtt gatgctttac tttctccatc
     9961 aagtcaggcg tggtgtgtac taatattaac gccatacctc ttgtgaaatc attttcattt
    10021 tcgtgggcca tcagtttttt gatgcgcact cttctgcgat tcaaaatatt tacgatccgg
    10081 ttcagtaccg aaaagttatt gtccgaatgt atggctaagg tgaaattctg tttcataact
    10141 agactttaga ttttagacag agacaaaaga ctcttgcctt ttcattttgt cgattttact
    10201 ttttcggtat ttccaaaata acttccgcta ctcccgcgcc tgctggtacc attggaaaca
    10261 cattgtcttc tttaccgact accgcctcta aaaagaaaga tgtttcggca tccagcattt
    10321 cctttaaagc agcttttaag tcctcacgtt tttcaacctt ccttgccggg atgtcatacg
    10381 cttctgcaag cttcacgaaa ttcgggttcg acatttcggt gaatgagtag cgtttctcat
    10441 ggaagagttg ttgccactga cgaaccattc caaggaagtt gttattcaac actaaaatct
    10501 ttaccggaat tttatactgc atgatggtac ctaactcttg taccgtcatc tgatatcctc
    10561 catctccaat tactgcgata acttgtttaa caggcattcc taacttcgct cctattgctg
    10621 cgggaagcgc aaaacccatt gtgcccgctc caccggatgt tacattggtg cgtggatttt
    10681 tatattcgta atagcgggat gttgtcattt gatgttgccc tacatccgtt acgatgatcg
    10741 cttcaccatt tgtcatttcg gacaagtgat gaattacttc ggccatcatc aattctcctg
    10801 taggattaaa ctcatgttta atgaccttct caaattcaat atccttcatc ttgtggaagc
    10861 ttccgatcca ctctggatat tgatttgaat cacacttcgt gatcaatgct gtcagcgctt
    10921 ctttcgcatc agcatgaact gctaccgttg ccggtatgat tttgttgatc tccgcgcgat
    10981 caatttcaat gtgaactact ttggcctgct tggcatactt cgatacgttt ccggttacgc
    11041 gatcatcaaa tcgcatacct actgcaatga gtacatcgca ctcattcgtg ttcacgttcg
    11101 gtccgtaatt tccatgcatg cccaggtatc ctacatagtt tggatgatcc gttggaaatc
    11161 cacccaagcc taataaagtt gaagccactg gaatacctgt cttttcagag aacgcaatca
    11221 atgcttctgt tgcgcctgat aacaaaatac cttgtcctgc aagaatgtaa ggacgcttcg
    11281 ctgcgttgat cagcttcgct gcttcttcta cttgcgaaag gtttaattca ggtttcggtt
    11341 tgtacgtgcg aatgtttttg cacggaacat attctgcctc cgcaatcatt tcgttctgtg
    11401 cattcttggt gatgtccacc agtacaggtc ctggtcttcc ggtacgtgcg atgtaaaatg
    11461 ctttcgcgat agccggtgca atatcttttg cttcggttac ttgtatgttc cacttcgtta
    11521 ctggaatcgt ggtgttgatc acatcaattt cctgaaacgc atccgtacct aacaagtgcg
    11581 caaacacttg gccggtgata cacacgattg gtgttgagtc aatcaacgca tcggcaagtc
    11641 ctgtaaccaa attcgtggca ccgggtcctg atgttgccaa agcaacgcct actttaccag
    11701 acacgcgtgc gaaaccttgt gccgcatgta cagcaccttg ttcatgacgc accaaaatat
    11761 gagtgagctt atcggcataa ccatagaggg catcatacac cggcatgatc gcaccgcctg
    11821 gataaccaaa caatgtattt acattctctg ccacaaggca ttgtaataat gtttcagaac
    11881 ctgaaatcat tttggcgggt gacgcttcct tcttggagct ggcctctccc gcagttttaa
    11941 ttttcgtcag taacgcatcc atcagctgct gtttttactt gttttgcata cttcaacaga
    12001 actccacttt tcactctcaa cttaggttgc acccaagctg ctttacgttt ggccagggtt
    12061 gcagcatcta ccttcaggtt gatggtacgg ccccggacat ctatttcaat cggatcattg
    12121 tcttctacta aaccaatcag tccaccgtca tacgcttcgg gtgtaatgtg gccaatcaca
    12181 aaaccgtgag aacctccgga gaatcttccg tctgtaatca acgctacact cttgcctaga
    12241 ccagcaccca taatggcgga tgtcggcttc aacatttcag gcatacctgg cgcacccttc
    12301 ggaccaacgt ttcgaatgac gactacgtca cccgctttaa ctcgtccttc agagatgccg
    12361 gcattcaaat caaactcact gttgaatgtg cgagcaggac cgttaaactt ctctccttct
    12421 ttaccggtga tcttcgctac cgatcctttc tctgccaaat tcccgtaaag gatttgtaag
    12481 tgacctgtcg ctttgatcgg attctctaat gggtgaataa catcttgcgt tgtgaaatca
    12541 agcgacttcg catccttcag attttctttt actgttttac ctgttacggt aatacagtca
    12601 ccatgaataa atcctttctc caataaatat ttcattactg at
//
LOCUS       DAOOQW010000051        12398 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_6307_length_12398_cov_5.241270, whole genome shotgun sequence.
ACCESSION   DAOOQW010000051 DAOOQW010000000
VERSION     DAOOQW010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 12398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12398
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6307_length_12398_cov_5.241270"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1358
                     /locus_tag="PK281_04715"
     CDS             <1..1358
                     /locus_tag="PK281_04715"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOY48680.1"
                     /translation="QLIRSSTFPFYGEPLTGLQLMGLLETRNLDFKNLILLSVNEGTL
                     PASKAHRSFIPHDISAFFGLPTYRERDAVFAYHFYRMIQRAERVWLVYNTETDELGKG
                     EQSRYITQLEEELNQEHTQITRETYVPHLHNTFENVIQIEKSPEVMERLRNRIGGKDP
                     YNYLSPTALSSFINCSLKFYFQYISGVKVEVTKEDEIGLDVMGTVAHEVLQKFYLPLE
                     KKVLKKEHLLAMKPRINEEVKLAFLHAGIEERDISGGSNLLSYKGVEKMLGNFIDAEI
                     RFVEELEKEGQVLTIEQIERDLQFKMKIKNGSSVNELLFKGRTDRIDRIGSSIRVVDY
                     KTGNVQDKDVKLDEQSEIALDPNKAKALQLLQYALMVPEEFQNNHVIPGIISLRRPSV
                     GFIGLKIERNEGLTPDERKETEIVFREIATALFDEDQAFEQTDDRARCVYCDFATICN
                     R"
     gene            1401..4655
                     /locus_tag="PK281_04720"
     CDS             1401..4655
                     /locus_tag="PK281_04720"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HOY48681.1"
                     /translation="MKKLASIKLLLLLCCFYCSGLNAQQYYFANYTLNDGLPHSRVNA
                     VLQAKNGYFWVATNMGISRFDGHFFVNFSAKDGLGDNKVTSVFELSDTELILGHENGK
                     LSYYNGSKFEPLQLDPDTRRIFCLTPDHSGNLWIGTQTTGAYKVSRKNLVANIINKRF
                     DRYDAAKGLSRDVTSVLHDSNDQLWFITDLGIKQLNKKTGTFDFYLPSGIDFVQFSAI
                     AEDGNKDLWLGTPSSGCWRYSQKQKKYISYTVENGMLGTNFVSVLQANSKGEMMIGTW
                     GGGVTLVKGDNVEILAEKNGLSENKIRCLGEDREGNLWIGTNQNGVSCYRGQSFRMYE
                     RSSNGTNTQIGAICTDSKGNRWFGTTNGIFFMPKGSQVSRRIDFLTGGEEIEVTSILE
                     DRRGLIWVSTWGEGVLLIHPGDYQVERFQGKIPYNDPITFNEQYVHTVYQASNGTIWI
                     SMLRGVAAFSPSTNQLHTYTKRDGLAENSTTDIQEDSQGRIWIGSATSGLSIYENGKF
                     NVVKETNPTIYPSISSISRDEQSNIWIATEGGGIYKFDGKKYTNYTTQDGLLSNYVGL
                     IESDLKGKIWLGTNKGICSWDPNTHYTIIHDKTDKNSRVETKPNAVHRDKENHLWFGT
                     INGVIEFDPGLITTNFVESVTRISAVKSFQDTLRKEGVALNYKKNYLSFYFNGICFSD
                     PEKVKYKYQLEGFDSTWQESSVNFVTYNNLAPGSYTFRVKSCNNDGVWNKVPVTFAFT
                     ITPPFWMTWWFYTISFIGLTVIIVSAVKFRERNLRLEKRRLEQVVQERTSEIIEQKEE
                     IENQRDELRVNSALIEQKNLSITDSIRYARRIQLATLPYSDVIHQSLPESFILYKPKD
                     IVSGDFYAFAKKDNKILIAVADCTGHGVPGAFMSMIGTNLFNQVINEKNITEPAAILN
                     ELDKGIERALKQEETDNHDGMDIVICSLDFENNVFEMAGANRPLWIIRAGKIEDHEHL
                     EQYGNSMQIIRPDKNPIGGFHQEKKEHFTNHPFNFYPGDKLYLCTDGYADQFGGEKGK
                     KLLSKRLRDYLIEIRHSPIQEQEGLLDDYYEEWRGNHEQVDDVLIIGIRYPEKG"
     gene            complement(4652..6355)
                     /locus_tag="PK281_04725"
     CDS             complement(4652..6355)
                     /locus_tag="PK281_04725"
                     /inference="COORDINATES: protein motif:HMM:NF016612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HOY48682.1"
                     /translation="MLIGVGKSKVIFKKLHLPMHGYGRLDHIAESIETDLHARAFAFR
                     KGDITLAIINLECCYITHHLKKAVVEAVLLAHPQSRLSMESLMLCSQNTHSAPGGYAH
                     YAFFNLVSCGFRPDVFNAYKNACVDALSKALKDLEPSNLFLNADWMPDDSDVAFNRSL
                     DAFNSNPENIEIDENHTQLAINRMMKQLRISIDEHTHKGLINWFGVQGTSISSKNSKI
                     HPDNKGYAASLLENDKTEDRHFVAAFCGETQADVSPNYHGRAKWWPRGRYEDEFKSAY
                     FSGFFQFEHARNLFESRDHQIPISDNLDFIQYYVDMSEITCDAKFTPDNQEHETGAAV
                     LGLSFLEGDDIDNPGIDSITATLIRGIDTYKNTIRKIPVLSSRKSRVRYKDLGSAHGP
                     KQLVVELQEKRVLGYSNLNQIPLPGVMTDIGDEMKRQYEIGALAEHTWAPVIVPIQIL
                     ILGEIAILAFPGEITTTAGNRLKQSVLTVLEKRGVIDVILTSNANEFSGYTTTFEEYQ
                     TQHYEAGFTLFGQYTLAAYQTAFTKMAEKLCEPIEKRHIPEAPQPPDFSKEELDKRTA
                     R"
     gene            complement(6485..7141)
                     /locus_tag="PK281_04730"
     CDS             complement(6485..7141)
                     /locus_tag="PK281_04730"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOY48683.1"
                     /translation="MSAPKSEIRLLIVDDHQMMIDGIKSLLLYESDFRIVAETTQPKD
                     VMGLLAKEQADIVIADVSMPGITGIELTKLIRNQYPTIHVLALSMHGDRDTISDMLEA
                     GISGYVLKNTGKDELLEALHKIASGGLFFSEDVTREMMRSVRSSRPTPVVEKIRLTPR
                     EIEIVKLIAAEYSNAQIGDALFISERTVETHRKNIFRKTATKSVAGLIKLVMEQGFLD
                     "
     gene            7293..7754
                     /locus_tag="PK281_04735"
     CDS             7293..7754
                     /locus_tag="PK281_04735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48684.1"
                     /translation="MISKLKSLVFTSILFLFIHQLAGQSTHYKILENNPGNKSLLIGI
                     SPFDVNGYSPDISIGWALRTNWMMNKFLQVDLDIRNAYLDINQNGVYSPDGLKKAHWY
                     QAGAAFNFRTHTLTKPVKVVLQHSRGSKYEHITYIRVPSEVKRISSVTVDL"
     gene            7751..8260
                     /locus_tag="PK281_04740"
     CDS             7751..8260
                     /locus_tag="PK281_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48685.1"
                     /translation="MTILNNHFIENDFTRNLQEGDIQGKTLQGDVVYFRDTINYESIN
                     YVANSTGIYGGLNFRSIRDLLIQTDSYGEKSTRVLSDFYLDFLFTPLVHYSLRPNSKQ
                     AMFKDIDISIPENKKKLLGWRVGYSYASGKTVGFTAKIEAGQQPGIPGNGFFMNVGMG
                     MSIRALGNK"
     gene            8365..8808
                     /locus_tag="PK281_04745"
     CDS             8365..8808
                     /locus_tag="PK281_04745"
                     /inference="COORDINATES: protein motif:HMM:NF016087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein NlpE N-terminal
                     domain-containing protein"
                     /protein_id="HOY48686.1"
                     /translation="MKALQSIIGIVCLGLVSCQSNTTEKNSAVTSNDVGVEKVFEGQV
                     PCADCAGINLRVSLKNDNDSAGSFRLTQTFKGTRDGDQTFSDSGSFSMRKGLDADADA
                     WVYVIRPGQPEETRYFEKRGDTALLSLGNDGQRIQSDLNYLLKRK"
     gene            8910..11195
                     /locus_tag="PK281_04750"
     CDS             8910..11195
                     /locus_tag="PK281_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOY48687.1"
                     /translation="MRRSFLFYVSIISGAAAAIWYVVKSGKTLESGRPIQSLPKVAAE
                     DLFQNGLDTFLANIHHPLAILILQIISIIFVARIFGLIFTRIGQPSVIGEITAGIVLG
                     PSLLGMYFPEYSLFLFPVESLGNLQFLSQVGLILFMFVIGMELDLSVLKNKAHEAVVI
                     SHASIVIPYTLGMGLAYYIYSEYAPPTVPFLSFGLFMGIAMSITAFPVLARIIQERGL
                     TRTKLGAIAITSAAADDITAWCILAAVIAIVKAGSFMSALFTIGFSLVYVVVMVYLIG
                     PFLKRLGSIYSNRETISKGIVALVFIILLASAYSTEIIGIHALFGAFLAGVIMPPNLN
                     FRKILIDKIEDVSLVLLLPLFFVLTGLRTQIGLLNEQHLWFVCGLVICVAVAGKFVGS
                     ALAAKIIGQSWKDSLSIGALMNTRGLMELIVLNIGYDLGILTPTVFAMMVIMALVTTF
                     MTGPSLSFINWIFKEKYVQPEIIPVKKEVPALKVLISFGLASSGRKILRLATQLYGTD
                     SKSVHYSAVHVTPSADVSLINVSEFEQQSFRPIRAEAEKMKIELESHYKLSTDLHKEV
                     TSMIEKEEINLLLIGAGKSLYTGSLLGNIVGVAKTLSPENLIGTITGANPLFRTDYLI
                     DDKARYFIEQSSCDVGVLIDRDFSDAGTLFLPLFGEEDAFMLNYARKFILNSKSDVSV
                     MDSFEQLAGDSVIREKFNRLKSEFPDRIKLLQVKTIGKEFLLQQELMLISYPSWKGLV
                     DSKSLWLEHVPSVLILRSRLQ"
     gene            complement(11170..>12398)
                     /locus_tag="PK281_04755"
     CDS             complement(11170..>12398)
                     /locus_tag="PK281_04755"
                     /inference="COORDINATES: protein motif:HMM:NF023128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOY48688.1"
                     /translation="SAILPAYYGFVAKGDEHGMVSFFGFQVVNTSLYAWAVSASFLIV
                     ALFSPILASIADYTGRRKFFMKLFTWIGGLSCAGLYFFTAENLEWGIIAFTLAGIGYS
                     GGLVFYNAWLPEIAPEGQEDHISARGYALGYIGGVILLIINLVMLLKPALFGIHIPGM
                     AARISFITVGIWWIGFAQYTFLYLPKRTSTKPNIVNPILNGYKELAKVWKEFRQTHVL
                     RIYLMAFFFTMMAVLTIMYMAANFGKKEIGLKDEVLIPTILIIQLVGVAGSIGFARLS
                     RRYGNMEAMMLSVIIWIGICVGAYYVTESIGFMILASVVGLVMGGVQALARSTYSRLL
                     PPTNDHTAYFSFYDVTEKMSVVLGTFLFGLLETLTGSMRASILLLGVLFMLGLYFLIR
                     LRSLKIPAITVNANAK"
BASE COUNT         3816 a         2276 c         2546 g         3760 t
ORIGIN      
        1 aacagctcat tcgttcgagt acttttccat tttatggtga gccattaacc ggattgcaat
       61 taatgggatt gcttgaaact cgaaacctcg attttaaaaa tctcattcta ttatctgtaa
      121 acgaaggaac actacccgct tcaaaagcac atcgatcctt tattccgcat gatatttcag
      181 catttttcgg attgccaaca tatcgtgaac gagatgccgt atttgcctat cacttttatc
      241 gaatgataca acgtgccgaa cgcgtttggc tagtgtataa taccgaaaca gacgaattag
      301 gaaagggaga acaatcacgc tatatcactc aactcgaaga agaactcaat caggaacaca
      361 cgcaaattac acgagaaacg tatgtaccac atttgcacaa cacatttgag aatgtcattc
      421 aaatagaaaa gtcgcccgag gtgatggagc gattgagaaa tcgtattggt ggaaaagatc
      481 cttataacta cctatctcct actgcattat catcattcat aaactgctca ttaaaattct
      541 atttccagta catcagcggt gtaaaagtag aggtaacgaa agaagatgaa attggcttag
      601 atgtcatggg aacggtggcg catgaggtat tgcaaaagtt ctatttgcct ttggaaaaaa
      661 aggtgctaaa aaaagaacat ttgctagcca tgaaacctcg cattaatgag gaagtcaaat
      721 tggctttttt acatgctggc attgaagaac gtgatatttc tggaggaagt aatttgcttt
      781 cttataaagg tgtggagaaa atgttgggca attttatcga cgctgaaatt cgttttgttg
      841 aagagttaga aaaagaagga caagtattga ccattgagca aatagaacgt gacctacagt
      901 ttaaaatgaa aattaaaaat ggtagttcgg ttaatgaact attattcaaa ggtagaaccg
      961 accgaataga tcgcatcgga tccagtattc gagtggtgga ttataaaacg ggaaatgttc
     1021 aggataagga tgtgaaatta gatgagcaat ctgagattgc actcgatccc aataaagcga
     1081 aagcattgca acttttacaa tatgcattga tggttccgga ggaatttcag aacaatcatg
     1141 ttatcccagg aattataagt ttgagaagac cttctgttgg atttatcggg ttgaaaattg
     1201 aacgaaatga aggtttaact cccgatgaaa ggaaggaaac ggaaatcgtt ttcagggaaa
     1261 tagccactgc cctatttgat gaagatcaag catttgaaca aactgatgat cgagcaaggt
     1321 gcgtgtattg cgattttgcg acgatttgca atcgatgaat tttttaagcg gctattttct
     1381 atcttaatcc cctcttgaaa atgaaaaagc tagctagtat taaattgctg ttgttgctct
     1441 gttgctttta ttgcagtgga ctaaatgcac aacaatatta ttttgccaat tacaccttaa
     1501 atgacggatt accacattcg cgcgtaaatg cagtattgca ggcaaaaaat ggatatttct
     1561 gggttgcaac caatatggga atttcccggt ttgatggaca tttctttgtg aatttttcgg
     1621 ccaaggatgg attaggagat aataaagtaa ccagtgtttt tgaattatcc gataccgaat
     1681 taattctcgg tcatgagaat ggaaaactga gttattataa cgggagcaag tttgagcctt
     1741 tacaattaga tccagataca cgaagaattt tttgcttgac acccgatcat tctgggaatt
     1801 tatggattgg aactcaaact accggagctt ataaagtttc acggaaaaat ctagtagcga
     1861 atattataaa taaacgtttc gatcggtatg atgcagctaa aggattatcg cgagatgtga
     1921 ccagtgtatt gcatgattca aatgatcaac tgtggttcat taccgacttg ggaatcaagc
     1981 aattaaacaa gaagaccgga acctttgatt tctaccttcc aagtggtatc gatttcgttc
     2041 aattttcggc aattgcggaa gatggaaata aagatttatg gcttggaacg ccttcatctg
     2101 gatgttggcg gtattcgcaa aagcagaaga agtatatttc ctatacggtt gaaaatggta
     2161 tgctgggaac caatttcgtt tccgttttac aagccaattc aaagggcgaa atgatgattg
     2221 gtacatgggg cggtggagtt actttggtta aaggcgataa tgttgaaatc ctagcagaga
     2281 aaaatggttt aagtgagaat aaaattcgct gtctgggtga agatcgcgaa gggaatttgt
     2341 ggataggtac taaccaaaat ggtgtttcct gctatcgcgg acagtccttt cgcatgtatg
     2401 aacgttcttc aaatggaacc aatacccaaa ttggagcaat ctgtaccgat tcaaaaggca
     2461 atcgttggtt tggaacaacc aatggaattt tctttatgcc taaaggatca caagttagtc
     2521 ggcgcattga ttttttaact ggtggtgaag aaatagaagt tacctccatt ttagaagaca
     2581 gacgtggact aatttgggtg agtacttggg gcgaaggagt tttgcttatt catccgggag
     2641 attatcaagt agagcgtttt caaggaaaaa taccttataa cgatccaatt acctttaatg
     2701 aacaatacgt tcatacagtt tatcaagcta gtaatgggac tatttggata tcgatgttac
     2761 gtggtgttgc agcgttttct ccttcaacca atcaattaca tacctataca aaacgtgatg
     2821 gcttagccga aaacagcaca actgatattc aggaagatag tcaaggacgc atctggattg
     2881 gttcagctac atcgggtttg agtatttatg agaatggaaa atttaatgtt gtaaaagaaa
     2941 cgaatcccac tatttaccct tccatttctt cgatttcgcg agatgaacaa agtaatatct
     3001 ggatagcaac agaaggtggc ggaatatata agttcgacgg taaaaagtac acgaactaca
     3061 ctactcaaga tggtttgctt tctaattatg ttggattaat tgaaagtgat ttaaaaggta
     3121 aaatttggtt ggggacgaac aaaggaattt gctcttggga tcccaataca cattatacca
     3181 taattcacga taaaaccgat aagaattcgc gtgtcgaaac caagcctaat gcagtacatc
     3241 gtgataaaga aaatcacttg tggtttggta caattaacgg tgttattgaa tttgatccag
     3301 gacttataac caccaatttt gtagagtcgg ttacgcgtat ttcggcagtc aagtcgtttc
     3361 aagatacctt gcgaaaagaa ggtgttgcct tgaattataa aaagaattat ttgagctttt
     3421 attttaatgg tatttgcttt agtgatccag agaaagtaaa atataaatac caactcgaag
     3481 gatttgattc tacttggcaa gaaagctccg tgaatttcgt aacctataat aatcttgctc
     3541 caggttcata cacgtttcgc gttaagtcgt gcaacaatga tggagtttgg aataaagttc
     3601 ctgtaacgtt tgcctttacc attacaccac ctttttggat gacttggtgg ttctatacca
     3661 tcagttttat tggtctaaca gtgattattg taagtgctgt aaaattcagg gaacgtaatc
     3721 ttcgtctaga aaagcgccga ttggagcaag ttgttcaaga gcgaacttcc gaaatcatcg
     3781 agcaaaaaga agaaatcgaa aatcagcgag atgaattacg tgtaaatagt gccttgattg
     3841 aacagaaaaa tctatcaatt accgatagta tccgctacgc gagaagaatt cagttggcca
     3901 cattgcctta tagtgatgtg atacatcaat ccttgcccga atcatttatc ctgtataaac
     3961 ctaaggacat tgttagtggc gatttttatg catttgccaa gaaggacaat aagatattga
     4021 ttgctgttgc cgattgcact ggacacggtg taccgggagc attcatgagt atgattggaa
     4081 ccaatttgtt caatcaggta attaatgaga aaaatattac tgaaccggcg gctattttaa
     4141 atgaactaga taaaggaatt gaacgggcgc taaagcaaga agagaccgat aatcacgatg
     4201 ggatggatat tgttatttgc tcattggatt tcgaaaataa cgtattcgaa atggctggtg
     4261 caaaccgtcc attatggatt attcgtgctg gtaaaatcga agaccatgaa cacctagaac
     4321 aatatggtaa ttcgatgcag ataattcgtc cagataaaaa cccaattggt ggatttcatc
     4381 aggagaaaaa ggaacatttc acgaatcatc catttaattt ctatccaggc gataaactct
     4441 atttatgcac cgatggttat gccgatcaat ttggtggcga aaagggtaaa aaattattgt
     4501 cgaaacgact acgcgattat ttaatcgaaa tacgccactc acccattcaa gagcaagaag
     4561 gcttacttga tgattattac gaagaatggc gaggtaatca cgaacaagtg gatgatgtac
     4621 tcatcattgg tattcgatac ccagaaaaag gttagcgagc ggttcgttta tctaattctt
     4681 ccttagaaaa atcaggtggt tgcggagctt ctggaatgtg ccgtttttcg attggctcac
     4741 agagtttctc agccattttt gtaaatgccg tttggtaagc agctaaggtg tattgtccaa
     4801 ataaggtaaa tccagcctcg taatgttgag tttgatattc ttcgaatgta gttgtataac
     4861 cgctaaattc gttagcgttg gacgttaaaa tcacatcgat cacacctcgt ttttctaata
     4921 ccgtaaggac cgattgttta agtcgattgc cagcggtggt agtaatttca cccggaaaag
     4981 ctaaaatggc aatttctcca agaattaaaa tctgtatcgg aacaatcact ggagcccaag
     5041 tatgttcagc taatgcacca atttcatact gacgtttcat ttcatctcca atatcggtca
     5101 ttacgcccgg tagtgggatt tgatttaagt tcgaatatcc aagaactcgc ttttcctgta
     5161 attcaaccac taactgtttg ggaccgtgtg cacttcctaa atctttataa cgaacacgac
     5221 ttttacgtga actcaatacc ggtattttac ggatggtatt tttatacgtg tcgattcctc
     5281 gtattaatgt ggcagtaatg gaatcaattc ctggattatc aatatcgtca ccttctaaaa
     5341 aagaaagtcc gagcacagct gcaccggttt cgtgttcctg attatcgggc gtaaacttgg
     5401 cgtcacaggt aatttccgac atatccacat aatattggat aaaatcgaga ttgtcgctaa
     5461 ttggaatttg atgatctcgc gattcgaata aattccgtgc atgttcgaat tgaaaaaagc
     5521 cgctgaaata ggccgattta aattcatcct catatcgccc acgtggccac catttagcac
     5581 gaccgtgata attgggagaa acatcagcct gcgtttcacc acaaaaagcg gcaacgaaat
     5641 gtcgatcttc tgttttgtcg ttctctaata atgaagcagc gtatccttta ttatcgggat
     5701 gtattttgga attcttgctc gatatagaag ttccttgaac accaaaccag ttaatgagcc
     5761 ctttatgtgt gtgctcatca atcgaaatcc gaagttgctt catcattcga ttaatagcca
     5821 actgggtgtg attttcgtca atttcgatgt tttcgggatt gctgttaaaa gcatccaacg
     5881 aacgattaaa tgcaacatcc gaatcatcgg gcatccaatc ggcattgagg aataaattgc
     5941 ttggttctaa atcttttaac gctttagaaa gtgcgtcaac acaggcattt ttgtatgcat
     6001 taaacacatc cggtcggaaa ccacaactca ccaaattgaa aaacgcataa tgcgcataac
     6061 cacctggtgc actgtgtgta ttttgagaac agagcattaa actttccatc gaaagtctcg
     6121 attggggatg agccaaaagg actgcttcaa caaccgcttt cttgagatga tgagtgatgt
     6181 agcaacattc gaggttgatt attgcgagtg taatatcacc ttttcgaaat gcaaaagcac
     6241 gggcatgtaa atcggtttcg atgctttcgg caatatgatc caaacgccca taaccatgca
     6301 tgggcagatg caatttttta aatataactt tcgattttcc gactccgatc agcatggcta
     6361 ataaaggcca aaactactac aaattgattg gataacggat ggattttatg caggtgattt
     6421 cacgcctata gaagatgaga aaattcagca catcagcctg atttaacagc gcttgcggaa
     6481 ttcttcaatc caaaaaacct tgttccatca ctaatttaat caacccagca acgcttttag
     6541 tagcagtttt acggaagatg ttttttcgat gtgtttcaac cgtgcgttca ctaataaaga
     6601 gagcatctcc aatttgcgca ttcgaatatt cagcagcaat gagctttaca atttcgattt
     6661 cacgaggagt aagtcggatt ttttctacta caggtgttgg acgagaagaa cgaaccgatc
     6721 gcatcatttc gcgggttaca tcttcactga aaaataatcc accggaagct attttatgta
     6781 aggcttccaa tagttcgtct ttacccgtat ttttcaaaac atagccacta attccagctt
     6841 cgagcatgtc ggaaatagta tcccgatcac catgcatcga taatgcaagc acatgaatgg
     6901 taggatattg gtttcgaatg agtttggtta attcaattcc agttattcca ggcatagaaa
     6961 catccgcaat gacaatatct gcttgttctt ttgccagtaa tcccattaca tctttaggct
     7021 gagtagtttc tgccacaatg cgaaaatccg actcatataa taggagtgat ttaattccat
     7081 caatcatcat ttgatggtca tccacaataa gcaagcgaat ttctgatttc ggagcagaca
     7141 ttttcatccg ttaatggcgg ctaaattagt gtgtttattg aacaatcatg aatatctacc
     7201 caatagcacg gattaattat gcccttagta gggtatttcg gtagcgcttt actgaaagta
     7261 gttttgaaaa ctgaaattta aagccaaccc tcatgatttc gaaattgaaa agtctagtgt
     7321 ttacgagcat attgtttttg tttattcatc aactagcagg acaatcgaca cattataaaa
     7381 tcttggaaaa taatccagga aataaatcct tgttaattgg tatctcacct tttgatgtga
     7441 acggatattc acctgatatt tcgattggtt gggctcttcg tacgaattgg atgatgaata
     7501 agtttcttca ggttgatttg gatattcgaa atgcctattt agacatcaat caaaatggtg
     7561 tttactcacc cgatggttta aaaaaagcac attggtatca agcgggagca gcatttaact
     7621 tcagaactca tacgttaacc aagccagtaa aagtcgtgtt acagcattca cgtggttcta
     7681 aatatgagca cattacctat atcagagtcc cttcggaagt aaaaagaata agttcggtta
     7741 cggtggattt atgacgattt taaataatca tttcattgaa aatgatttta cacgcaatct
     7801 tcaagaaggt gatatacaag gaaaaacgct tcagggagat gttgtttatt ttagagatac
     7861 cattaattat gagtcgataa attatgtagc taattcgaca ggtatttatg gaggtttgaa
     7921 ttttcgctcc attcgcgatt tgttaataca aacagattcc tatggtgaaa aatcaactcg
     7981 tgtactttcc gatttttacc ttgatttttt atttactcct ttggttcact atagtttgcg
     8041 acctaattcg aaacaagcca tgtttaaaga catagatatt tctatcccag aaaataaaaa
     8101 gaaattactc ggctggcgtg ttggatattc atacgcatcg ggtaaaaccg ttggatttac
     8161 tgcgaaaatc gaagctggtc aacaacccgg aattcccgga aatggatttt tcatgaatgt
     8221 tggaatgggc atgtcgattc gtgctttggg gaataaatag ttgattattt aaattccatt
     8281 ctggaaaatt tcaatttaga attgagcttt catttcggga attaaatctg gtcgtatatt
     8341 tccgacatac ttaatgctca aatcatgaaa gcacttcagt cgattattgg aatagtatgt
     8401 ttgggattag tatcctgcca gtcgaatact acggagaaaa actcagcagt tacttctaat
     8461 gatgtaggcg tagaaaaagt atttgaagga caagtccctt gtgccgattg cgcaggaatt
     8521 aatttgcgtg ttagcttaaa aaatgacaat gattctgctg gtagttttcg attaacacaa
     8581 actttcaaag gaacacgcga tggtgatcaa acattcagcg acagtggttc ctttagcatg
     8641 agaaaaggtt tagatgctga tgcagatgct tgggtttatg tgattcgtcc cggacagccc
     8701 gaagaaacac gctatttcga aaaacggggc gatacagcac ttttgagttt aggaaatgac
     8761 ggtcagcgta ttcaatcgga tttgaattat ctgctaaaaa gaaaataagt tctcccgctg
     8821 tgttacgtgt attagctgat tattaattta tactcgagat taaaaaaggg aaataagtcc
     8881 tgtatcttcg cccttatttt cccaacccga tgcgtcgctc gtttttgttt tatgtcagta
     8941 ttatttccgg tgctgcagcg gccatttggt atgtagttaa gtcgggaaaa acattggaat
     9001 cgggtcgtcc aattcaatcg cttcccaaag ttgctgccga ggatttattt caaaacggac
     9061 tggatacatt tcttgccaat atccatcatc cgcttgccat tcttatttta caaatcatct
     9121 ctatcatttt cgttgcccga atttttggtt tgatatttac ccgaatcgga caaccatctg
     9181 tcattggaga aataactgca gggattgttc ttgggccatc gcttcttgga atgtattttc
     9241 cagaatattc gcttttcctt tttccggttg aatcgcttgg gaatcttcag tttttaagtc
     9301 aggttggact aattcttttc atgtttgtta ttggaatgga attggacctt tcggtgttaa
     9361 agaataaagc acacgaagcg gtagttattt cacatgccag tattgtaatt ccgtatacgc
     9421 tgggaatggg gctcgcatac tatatatata gtgagtatgc accaccaact gttccttttc
     9481 tttcgtttgg attattcatg ggaattgcca tgagtataac tgcatttccg gtattagcac
     9541 gaatcattca agaacgtgga ttaacccgaa ccaaactcgg agctattgct attacgagtg
     9601 ctgccgctga tgatattact gcttggtgca ttttagctgc agtaatcgct atcgtaaaag
     9661 ctggctcatt tatgtcggca ttatttacta ttggtttctc cttagtatat gtagtggtta
     9721 tggtttacct cattggacct ttcctcaaac gattgggtag catttattcc aaccgcgaaa
     9781 ccatttctaa aggtatcgtt gcactcgtat ttatcatttt acttgcatct gcctattcga
     9841 ccgaaattat tgggattcac gcattgtttg gcgcattcct cgcgggtgtt atcatgccgc
     9901 caaatttgaa tttccgaaaa atccttatcg ataagattga agacgtttcg ctcgtcctgt
     9961 tattgcctct tttctttgta ttaacgggtt tgcgtacgca gataggctta ctcaatgaac
    10021 aacatttgtg gttcgtttgt ggattggtta tttgtgttgc tgttgcagga aaattcgttg
    10081 gttcggcctt ggctgcaaag attatcggac aaagctggaa agacagtttg tcgattggcg
    10141 ctttaatgaa cacgcgcggt ttgatggaat taatcgtatt aaatattgga tacgatttag
    10201 gaatcctcac tcctactgtg tttgctatga tggtgataat ggcgttggta accactttta
    10261 tgacaggtcc gtccttatca ttcatcaact ggatattcaa ggaaaaatat gttcaaccag
    10321 aaattattcc ggtaaaaaag gaagttccgg ctttaaaagt tttgatatcg tttggattag
    10381 ctagtagtgg aagaaaaatt ttacgtttag ctactcaatt atatggtacc gattctaagt
    10441 cggtgcacta ttcggctgtt cacgttacac cgagcgctga tgtgagtttg atcaatgtga
    10501 gtgaatttga gcaacaaagt ttcagaccga ttcgcgcaga agctgagaag atgaaaattg
    10561 agctggaatc gcactataaa ctcagcaccg atttacacaa ggaagttacc agtatgattg
    10621 aaaaagagga aatcaacctc ttattaattg gagctggaaa atcgctgtac actggatctt
    10681 tattgggtaa tattgttgga gttgctaaaa cattatcacc cgaaaacctg attgggacaa
    10741 tcaccggggc gaatccgctt ttccgcaccg attacttgat tgatgataaa gcacgttatt
    10801 ttattgagca atcaagttgt gatgtaggag ttttaattga tcgcgatttt agtgatgcgg
    10861 gcactttgtt tcttccttta tttggagaag aagatgcgtt tatgttgaat tatgctcgca
    10921 agtttattct gaattctaaa tcagatgttt cggtgatgga ttcgtttgag caattagcgg
    10981 gagattcggt aatacgtgaa aaattcaacc gacttaaatc cgaattccct gatcgaatca
    11041 aacttctcca agttaaaacc attgggaaag aattcctttt gcaacaagaa ttgatgttaa
    11101 tcagctaccc aagttggaaa ggtctggtgg acagcaaaag cttatggctt gaacatgtac
    11161 cgagcgttct tattttgcgt tcgcgtttac agtaattgct ggtattttca aactacgcag
    11221 gcgaattaag aagtacaatc ccagcataaa caacacaccc aataaaagta ttgatgcacg
    11281 cattgatccg gtgagcgttt cgagtaatcc gaaaaggaaa gttcctaata caactgacat
    11341 tttctctgtt acatcataaa agctgaaata ggctgtatga tcgttcgttg gaggtaataa
    11401 tcgcgaatag gtagaacgtg ctaaagcttg cacaccgccc attactaaac caaccacgct
    11461 ggctaaaatc ataaatccaa tcgattcggt aacgtaataa gctcccacgc agattcctat
    11521 ccagataatg accgaaagca tcatagcttc catattccca taacgacgcg ataatcgagc
    11581 gaaaccaatc gatccggcta cgccaacaag ttgtataatg agaatagttg gaatgagtac
    11641 ttcatctttc aagcctattt cttttttccc aaagttggca gccatataca tgatagtcaa
    11701 tacggccatc atcgtaaaaa agaaagccat caagtaaata cgcaacacgt gagtttgacg
    11761 gaattccttc catactttgg ctaattcctt atatccattg agaataggat taacgatgtt
    11821 aggtttagtg cttgttcttt taggtagata tagaaacgtg tactgtgcaa atccaatcca
    11881 ccaaatacct accgtgataa aacttatacg cgctgccata cctggaatat gaattccaaa
    11941 tagtgctggc tttagtaaca tcaccagatt aatgataagc aaaatcactc caccgatata
    12001 tcctaaagca tatccacgtg cagaaatatg gtcttcttgt ccttcgggag caatttcggg
    12061 caaccaagca ttatagaaaa ccaatccacc actgtatcca attcctgcta gagtaaatgc
    12121 aattatgccc cattcgaggt tttcggcggt gaaaaaatat aatccagcac aagacaatcc
    12181 tccaatccat gtaaacagct tcatgaaaaa cttcctgcgt cctgtataat ctgcaatcga
    12241 agctagaatt ggagaaaaca atgcaactat taggaaagaa gccgaaacag cccaagcata
    12301 caaagatgta ttgaccactt gaaaaccaaa aaaggatacc attccatgtt catcaccttt
    12361 cgccacgaag ccataataag caggtaaaat agccgaag
//
LOCUS       DAOOQW010000052        11886 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_6685_length_11886_cov_6.926126, whole genome shotgun sequence.
ACCESSION   DAOOQW010000052 DAOOQW010000000
VERSION     DAOOQW010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 11886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11886
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6685_length_11886_cov_6.926126"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="PK281_04760"
     CDS             <1..507
                     /locus_tag="PK281_04760"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY48689.1"
                     /translation="GEYNAQPEFLIGGQKVISISYQDSAIVKYYEPTPLCRIHYYELT
                     DSVICIDKIVDVVSGAFEQTVTVIGGECFTYDPTGMINFDKSNYFRLTPNPTSSEVNI
                     DLNMAKALDATIRIMNAQGQLVYTQQVKQAEKQRVTVNTSELANGIYLVNLLTEKGLI
                     TKKLVVNH"
     gene            complement(574..1308)
                     /locus_tag="PK281_04765"
     CDS             complement(574..1308)
                     /locus_tag="PK281_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48690.1"
                     /translation="MLKKALILTGVSLSATLMAQIPEPVNSYQKSYFATVSEGILSAG
                     NLGKVALYDPTKPIPSNVLDFGDQATPVIRYSTFLHLGEQLHVNFSNTFGFYTGIGMR
                     NIGMINRLSDSVKVKQRVYSVGIPIALKIGNMGKKTYAAFGAEMELFFNYKQKTFKGS
                     SGRGEKVEKFNEWFSKRTELFNPSVFVDFNFGKGSYIKVRYYLNNFLVQTKQNYTLNN
                     VKYGFFPESSQLFALSFGRVIGAKRK"
     gene            complement(1328..2518)
                     /gene="kbl"
                     /locus_tag="PK281_04770"
     CDS             complement(1328..2518)
                     /gene="kbl"
                     /locus_tag="PK281_04770"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HOY48691.1"
                     /translation="MYGSYQQYLQKELASIEEAGLFKKERIILNPQGADIRVSSGQEV
                     VNFCANNYLGLSSHPAVIKAAHETLDSHGFGMSSVRFICGTQDIHKELEKKISAFLGT
                     EDTILYAAAFDANGGVFEPLFGAEDAIISDSLNHASIIDGVRLCKAVRYRYNNNDMAD
                     LEARLIESQAQRFRIIVTDGVFSMDGYIAQLDKIVELAEKYNALIMVDDCHSTGFLGK
                     TGRGTAEHCGVFGKIDIITGTLGKALGGAMGGFTSGKKEIIDLLRQRSRPYLFSNSLA
                     PSIVGAGIAVLDLLSSSTHLRDKLMDNTTYFRAKMKEAGFDIKEGVHPICPIMLYDAR
                     LSQVFAEKLLAEGIYVIGFYYPVVPKDQARIRVQISAAHERHHLDKAIAAFIKVGKEL
                     GVIQ"
     gene            2712..4028
                     /locus_tag="PK281_04775"
     CDS             2712..4028
                     /locus_tag="PK281_04775"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOY48692.1"
                     /translation="MTAISSAKSSSKIFFPGLDGLRFIAAAFVILFHVEYKRSWFGIS
                     NFLHINFFYTIGYYGVTLFFVLSGFLITYLILKEVEKEGKVNVIHFYIRRALRIWPLY
                     FFIILTGFFLWPNISGMAIPGFEELWDKLDWKIFLLYAFFLSPLVLVWVGNIPYLDQT
                     WSVSVEEQFYLLWPILIRFYYKKIVRVLFLVIFIMLAIKTGILLINHFTGRGSKFLIL
                     AELSRFGCMATGGLAAYAFFKNKESLLRFVYRTDVLIITLAFTASLLIWGFSTVLVAP
                     FLILIGLLVFNYTRKNQLAHGIWFLIGSLLMLALLIVSLAGLADFQLFETYKHEIIAF
                     PFALIILNISANPEKFGILQHPVMQYLGRISYGVYMYHNIMITVSVKVLLAFYPAPES
                     HVGHVLHYIIAFALAISCSALSYALLEKPFLKMKKRFTKIRSGANG"
     gene            complement(4029..4574)
                     /locus_tag="PK281_04780"
     CDS             complement(4029..4574)
                     /locus_tag="PK281_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009087385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dps family protein"
                     /protein_id="HOY48693.1"
                     /translation="MAAKKASSSKKTTASKKPATRISAARTTNSIGLDSVQGKELAEK
                     LNDLLANYQVFYMNARGFHWNIKGDKFFELHLKFEELYNNLVLKVDELAERVLTLGHV
                     PMHGYDDYLKTSVVKPVKNVSDGKKAIQSILDSFKVLITKQRVLLDLSDVANDEGTNA
                     LMSDYIREQEKLIWMYSAVLG"
     gene            4744..5922
                     /locus_tag="PK281_04785"
     CDS             4744..5922
                     /locus_tag="PK281_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007095203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOY48694.1"
                     /translation="MSKDRFQGHDYYQIDDLLTDEQKLIRDTVRAWVKKEVTPIIEDH
                     AQRAVFPKHLIKGLGEIGAFGPYIPVEYGGGGMDQITYGLIMQEIERGDSGIRSTASV
                     QSSLVMYPIYKYGSEEQRHKYLPKLATGEMMGCFGLTEPDSGSDPGSMITNIKDMGDH
                     YLLNGAKMWISNAPFADIAVVWAKNEAGKIRGIIVERGMEGFTTPETHDKWSLRASAT
                     GELVFDNVKVPKENLLPNVEGLKGPLGCLNSARYGIAWGVIGAAMDCYDSALRYSKER
                     IQFGKPIASFQLQQKKLAEMITEITKAQLLCWRLGVLRNEDRATAAQISMAKRNNVNM
                     ALEIAREARQMHGGMGITGEYPIMRHMMNLESVVTYEGTHDIHLLITGMDVTGINAFK
                     "
     gene            complement(5989..9678)
                     /locus_tag="PK281_04790"
     CDS             complement(5989..9678)
                     /locus_tag="PK281_04790"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HOY48695.1"
                     /translation="MLFLINSRVAVEPCIFAKVEIQTTGSGTELYCFMRINLIALLLG
                     FLVSCSSVEERKGPPILPEKSNSKPVQTVLAGKPRIVNLKNCPAPEQAILSNRNLFDP
                     EGIETAAASANFEVPLKKFSSEDGLGINAVICGFCDSRGNLWFGTNGGGVSCYNGHSF
                     VNYTANQGLADNIVRCIAEDKHGNMWFGTRGGGISCYDGLRFRNYSTSEGLTNSDIRA
                     IAIGKNGVVWIGTEGGGVNLLKPQNKGYSSKLEPFNTQINSRSEFIQCIYEDKRGMIW
                     IGSKSSGAQAYDPSGNNPNPFLALNARLGLADSSVTCMLQDKSGKIWIGTNSGLCRFN
                     GKRMDHFTQVDGLADNSVLCLFEDDKGMIWIGTQNGGVSCYDPTVEPLSGRKAFRNLT
                     TGNGLPNNQIYSITEDHSGNLWFGSWGGGVVQYSGNSISHLMTNEGKENIKVVSLAED
                     KAGHLWFTNQTQSLIRYDGEQIRSFSNSLLNRFGVVRGSIYDSKGSIWFATSGAGVCR
                     FDGKTIFSFTVGQGLAGNNTRSVFEDSKGRIWVGSFKQGVSCIAGDSILTYTTEQGLP
                     NNEVWNIMEDRKGNIWFSTHGGGASCFTGKEFLNYSMDQGLPGNVIWNINQDDAGNLW
                     FASDGGGVSRFDGKSFLSISVAQGLGDDVILASKVAQDGSIWLGTNKGFSVIRMFRSE
                     QDSLNTPESRISATNSLTNQELQQKGWKPEVEIYNKRTGFPVEDFYPNSMYFDRRGVV
                     WAGTSEGLVRFDYRQPITGRKSGLLELHAIRVNNERICWQDLREDTSQTNSLFVPAYR
                     NEEVLVFGRVLTEAERQEFKKKFSGIEFEHISAFYPIPKKLSLPYQFNNITIEFGAIE
                     TARPSLVRYQYKLEGYDSDWSPLSDKSSATFGNIPEGDYVFLVKSRSPEGVWTKPLEY
                     AFTVRAPWYRTWWSLGLYLILGASFLFVMFRWRTMNLVRDKKKLEHQVTERTTEVVQQ
                     KEIVEFKNREILASIKYAKRIQTAILPSARSIRQNLPNSFILYLPKDIVAGDFYWIEK
                     VGERVLFAACDCTGHGVPGAMVSVVCNNALNRAVREFGLVRPAAILDKVADLVIENFS
                     TSEDSIEDGMDISLCALNLVTMQLEWAGANNPLWIIREGNLTQYKPDKQPVGLYENPL
                     PFTNHTIQLESGDQLYVFSDGYADQFGGEELEKKLTRKRFRELILSVSKNDIRAQGDY
                     LESFLVNYRNDRMQIDDILVIGVQV"
     assembly_gap    9917..10016
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10017..11701
                     /locus_tag="PK281_04795"
     CDS             <10017..11701
                     /locus_tag="PK281_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48696.1"
                     /translation="PTNTTLVIGRVVDESGNPVAGSSVSAGSISSISNSDGTFVLSGV
                     SGTSRVFVSANKSGYFKGSRGAIAENNGITKIEITLLENTPNFIANAGSSNSLLLSNG
                     SGVELASNTVQDQSGASVSGSFQVSIVHLDPADPDFENKVPGGDLIGVDAASQELQLF
                     SYGMLLVQMTDNSGNELQLASGSTATIRMKVPTSQLSTAPSSIPLWHFNETSGKWEEQ
                     GSASLQGDTYVGTVSHFSSWNCDHPGSRATVRGLVRDCNGNVLPGIRVRVGQTSAFTD
                     ASGQYETFAPAGVDFDVAVESPELGIFSTPNTVSSLTAGSVFDAPAISVACPGYVTMS
                     LACSSGSTFNASVSVNWGASQSVFGSTQTPGTFKIAVPTNGQTANIIVTSLTNGDSYS
                     VSVTLPTSANQEISAGTFEMCNTSSSGNTIQSSFTINGDGFNNQTITINAMPVICYSV
                     FNTSDFSTVGIAMETYGLTVIFPGNTVNTYEHNDSTDVYATITNNGNSWVADSVLTIN
                     VTEFGPVGGRVKGNFNGIFRRVDYNQTSGEVNFFYTTVTNGHFEFIRRPDEN"
BASE COUNT         3454 a         2684 c         2088 g         3560 t
ORIGIN      
        1 ggagaatata atgctcaacc agagttttta attggcggac aaaaagtaat cagcatcagc
       61 taccaagatt cggctattgt aaaatactac gagccaactc cattatgccg cattcactat
      121 tacgagttaa ccgattcggt tatttgtatt gataaaattg tagatgtggt gagtggtgca
      181 tttgagcaaa cagtgactgt tattggcggt gagtgtttca cttacgatcc aactggaatg
      241 atcaatttcg ataaatcgaa ttacttccga ttaacaccga atccaacatc cagcgaagta
      301 aacatcgacc tcaacatggc aaaagctctt gatgcaacaa ttcgaattat gaatgctcaa
      361 ggacagctcg tttatacaca acaagtaaaa caagccgaaa agcaacgtgt taccgttaac
      421 acttccgaat tagcaaatgg tatttactta gtgaatctgc taaccgaaaa aggattgatt
      481 actaagaaac tggtcgtgaa tcactaatca gatttaacaa cataaaaaaa ccccggaacg
      541 actaatcatt ccggggtttt ttatttgata ttattacttg cgttttgctc caattactcg
      601 tccgaacgac aaagcaaaaa gctgagatga ttcaggaaag aagccatact taacattatt
      661 caacgtatag ttttgcttcg tttgaaccaa aaagttattg agataatagc gaaccttgat
      721 gtaggatcct tttccaaagt taaaatcaac aaataccgaa ggattaaaca actcggtgcg
      781 tttagaaaac cactcattga atttttccac tttctcacca cgaccgctgc ttcctttgaa
      841 tgtcttttgt ttgtagttaa agaaaagttc catttcagca ccaaaggcag cataggtttt
      901 ctttcccatg tttccgattt taagcgcgat tggaattccc accgaataaa cgcgttgttt
      961 tactttaaca ctatcactca atcgatttat cataccaata tttcgcatgc caattccagt
     1021 gtaaaagccg aaagtattgc taaaattcac atgtagctgt tcgcctaaat gtaaaaaggt
     1081 agaataacga ataacgggtg tagcctgatc accaaaatct aaaacattag atggaatcgg
     1141 tttagtagga tcgtataaag caactttacc caagtttcct gcagataaaa taccttccga
     1201 aacagtagca aaataacttt tttgataaga attaactggc tctggtattt gagccattag
     1261 tgtcgcagat aaagaaacac cggtcaatat gagggctttt tttaacatgt ccgaatgagt
     1321 ttaagtttta ttgaatgaca cctaattctt tgccaacttt tataaatgct gcaatcgctt
     1381 tatccaaatg atgacgttca tgtgcagccg aaatttgaac tcgaattcta gcttgatcct
     1441 taggaacaac cggataataa aacccaatta cataaattcc ttctgctagt aatttctcag
     1501 caaacacctg actcaaccgg gcatcataca acatgattgg acaaatcgga tgaacacctt
     1561 ccttgatatc aaatccagcc tctttcatct ttgcacggaa atatgtcgtg ttgtccatca
     1621 acttatctcg taaatgagta gatgagctca ataaatccaa aacagcaata ccagccccga
     1681 ctatggatgg tgctaaagaa ttggaaaaca aatacggacg agaacgctga cgaagtaagt
     1741 cgatgatttc ttttttcccg gaagtaaatc ctcccatggc accaccaaga gcttttccta
     1801 atgttccggt gatgatatca atcttcccaa atacaccaca atgctcggct gttccacggc
     1861 cagtttttcc aaggaaaccg gtgctatgac aatcatctac catgatgagt gcattgtatt
     1921 tttcagccaa ctcaacaatt ttgtcaagtt gagcaatata tccatccatc gaaaaaacac
     1981 catcggtaac tatgatgcgg aagcgttgtg cttgagactc aataagtcga gcttccaaat
     2041 cggccatgtc gttgttgtta taacgataac gaacagcttt acacaaacgc acaccatcaa
     2101 ttatagatgc atggtttaat gaatcagaaa tgatcgcgtc ttcggcccca aataagggtt
     2161 caaaaactcc tccatttgcg tcaaacgcag ctgcatacaa aatggtgtct tcggttccta
     2221 aaaaggcaga tatttttttc tctaattctt tatggatatc ctgtgttcca caaataaatc
     2281 gaacactact catcccaaac ccatggctgt cgagtgtttc gtgcgccgct tttataactg
     2341 caggatgtga agaaaggccc aaataattat tcgcacaaaa attaacaacc tcttgtccgc
     2401 tacttacacg tatgtcggct ccttgtggat ttagaattat ccgttctttt ttaaataacc
     2461 cagcttcttc aatggaggca agttcttttt gtaagtactg ttggtaagac ccgtacatgt
     2521 gcaggagttt ttggtgaata tccgcgcaat ataaagcaga ttaagttttc ttttagaata
     2581 acaaccctaa gattactgtt aatcctcaaa accagagcat ccccattttg ctatatttgc
     2641 cccttaacca aatacgctgt ggctgcgatg cactatttag tttcgccctt ttgccttcta
     2701 gactctgtta aatgacagcc atttcatcag ctaaatcctc atcaaaaatt ttcttcccag
     2761 gactcgacgg tctgcgtttt attgctgcag catttgttat cctatttcat gttgaataca
     2821 agcgtagttg gtttggaata agcaactttc ttcacattaa ctttttttac acgataggat
     2881 attatggtgt gaccttgttt tttgtgttga gcggttttct tatcacctac ctcatcttga
     2941 aagaagttga aaaagaggga aaagtcaatg tgattcattt ttacatccgg cgcgctttga
     3001 ggatttggcc gctttacttt ttcatcatac taacaggatt cttcctttgg ccgaatattt
     3061 ctgggatggc tattcctggc tttgaagaac tttgggacaa actcgattgg aagatttttc
     3121 tgctctatgc attcttcctt tctccgctcg tattagtatg ggttggaaac ataccttacc
     3181 ttgatcaaac ttggtcggtt agtgttgagg aacaattcta tctcctttgg cccatcctta
     3241 ttcgattcta ttacaagaaa atagtacgag ttctattctt ggttattttc atcatgttgg
     3301 caataaaaac cggcatcttg ctcattaatc attttacagg aagaggttct aaatttctaa
     3361 ttttagccga actcagcagg tttggttgta tggctactgg cggattggct gcctacgctt
     3421 ttttcaaaaa caaagaatcg ttattacgat ttgtttatcg caccgatgtt ctaattatta
     3481 ccctagcatt tacagctagt ttacttattt ggggcttctc cactgtactt gtagctcctt
     3541 tcttaatcct gatcggactc cttgttttta actatacacg aaaaaatcaa ttagcacacg
     3601 gcatttggtt tctcattggc tctttgttaa tgctcgcttt actcattgtt tcattagctg
     3661 gattagctga tttccaactt ttcgaaacct acaaacacga aattattgct ttccctttcg
     3721 cacttatcat cttaaacata agcgcaaacc cagaaaaatt cgggatttta caacatcctg
     3781 ttatgcaata cttggggcgc atttcttatg gcgtttacat gtatcacaac atcatgatta
     3841 cagtatcggt caaggtctta cttgcttttt atcctgctcc agaatcacat gttggacatg
     3901 tacttcacta catcatagca tttgctttgg cgatttcctg tagcgcactt tcttatgcgc
     3961 tgctcgaaaa accattcttg aaaatgaaaa aacgcttcac taaaattcga agtggagcca
     4021 atggttaatt atcccaaaac agccgagtac atccaaatta atttctcttg ctcacggata
     4081 taatcactca tcagagcatt tgttccttca tcgttcgcca catccgacaa atcaagcaat
     4141 acgcgttgtt tggtgattaa aaccttgaaa gaatctagaa tcgactgaat ggcttttttg
     4201 ccatcactta cattcttaac aggtttaaca acagatgttt taaggtaatc gtcataacca
     4261 tgcatgggaa catgtcctaa ggttaaaact cgttcagcta gttcatccac ttttaaaaca
     4321 aggttgttat agagttcttc gaattttaaa tgcagttcga aaaatttatc gcccttaata
     4381 ttccaatgaa atccacgggc attcatgtaa aacacttggt aattcgccaa taagtcattg
     4441 agcttttctg ccaattcttt tccttgaact gaatctaaac caatagaatt tgtcgtgcgg
     4501 gcagccgaaa ttctggttgc aggcttttta cttgctgtgg tttttttact ggaacttgct
     4561 tttttcgctg ccattggctg atttattacg ggtgggaaat gaaatgtatt cgctgctaaa
     4621 ataacgcaat cagcgcttca agtcatgtga ttttattgaa acaaaacaat cctctcacaa
     4681 acctgtttat tcacacttcg aaaaacctac ttttgcaagc gcaaaatcaa atcaaccttc
     4741 aacatgagta aagacaggtt tcaaggccac gattactatc aaatcgacga tctacttacc
     4801 gatgaacaaa agctaattcg cgatactgtt cgtgcttggg taaaaaaaga ggtcacacca
     4861 attatcgaag atcacgctca acgcgcggtt tttcctaaac acctaattaa aggtctcggc
     4921 gaaatcgggg cttttggacc atacattcca gttgaatacg gtggtggagg aatggatcaa
     4981 attacctacg gtcttattat gcaagagatc gaacgtggtg atagcggaat ccgttcaacg
     5041 gcttcggtac aaagctcttt ggtgatgtat ccgatttaca aatacggttc tgaagaacaa
     5101 cgtcacaaat accttccaaa actcgcaaca ggcgaaatga tgggctgctt tggtcttact
     5161 gaacctgatt ctggctccga tccaggaagc atgatcacca acatcaaaga catgggcgac
     5221 cattatttgc tcaacggtgc taaaatgtgg atttctaacg cgccttttgc tgatatcgct
     5281 gttgtttggg ctaaaaacga agctggaaaa attcgcggaa tcatcgttga acgcggaatg
     5341 gaaggtttca ctacacccga aactcacgac aaatggtctc ttcgcgcttc agctacagga
     5401 gaactcgtgt tcgataacgt taaagttcca aaggaaaacc tacttcctaa cgtagaaggt
     5461 ttgaaaggtc ctttaggttg cttaaactct gctcgctatg gtattgcttg gggtgttatt
     5521 ggcgctgcaa tggattgcta cgattctgca ttacgttatt ctaaagaacg tattcaattt
     5581 ggtaaaccaa tcgcaagctt ccaacttcaa caaaagaaat tggccgaaat gattaccgaa
     5641 atcaccaaag ctcaattgct ttgctggcgc ctaggcgttc tgcgtaacga agatcgcgct
     5701 actgccgctc aaatttccat ggcaaaacgt aacaacgtga acatggccct agaaatcgct
     5761 cgcgaagcac gtcaaatgca cggcggtatg ggaataactg gtgaatatcc tatcatgcgt
     5821 cacatgatga acctcgagtc ggttgtgact tatgaaggaa ctcacgatat ccatttactt
     5881 atcacaggaa tggatgttac tggcattaat gcttttaaat aacaggctag gattaagttt
     5941 ttttcctact cttattgcac ttcaaaaacc cggtttagcc gggttttttt atacctgtac
     6001 gccaattacc aaaatgtcat caatctgcat acgatcgttt cggtaattca ccaaaaagga
     6061 ttctaaataa tcgccctgtg cccgaatatc atttttcgaa acggataata tcaattctct
     6121 aaaacgcttt cgtgttaatt ttttctctaa ctcttctcct ccaaactgat cagcatatcc
     6181 atcactaaaa acatacagtt gatctcccga ttcaagctgt atagtatgat ttgtaaacgg
     6241 cagcggattc tcatacaaac caactggttg cttatctggt ttgtattgcg tcaaatttcc
     6301 ctcgcggata atccataacg gattgttcgc tccagcccat tctaattgca tagtaaccaa
     6361 attcagcgcg caaagagaaa tgtccattcc atcctcaata gagtcttcgc tggtagaaaa
     6421 attctcaatt accaaatccg ccactttgtc taaaatggct gccggtctta ccaaaccaaa
     6481 ctccctcaca gctctgttca aagcattatt acaaaccacc gaaaccatcg cgcccggaac
     6541 tccatgtccg gtacaatcac atgcagcaaa taaaactcgc tctccaactt tttctatcca
     6601 gtaaaaatca cccgctacaa tgtcctttgg caaatacaag ataaacgaat tgggaagatt
     6661 ttgtcggatc gatcgtgccg aaggtaaaat cgccgtttga atacgcttag catatttaat
     6721 tgaggctaaa atctctcggt tcttaaattc tacaatctcc ttttgctgaa caacttctgt
     6781 cgtacgctca gtaacttgat gctctaactt tttcttgtct cgaaccaaat tcatcgttcg
     6841 ccacctaaac ataacaaaca agaaacttgc acccaaaatc aaatacaaac caagcgacca
     6901 ccaagtgcga taccaaggtg ctctaactgt aaaagcatac tctaaaggtt tggtccaaac
     6961 tccttcggga ctgcgcgact tcactaaaaa cacataatct ccttcaggaa tatttccaaa
     7021 agtagcgctc gatttatcac ttagcggact ccaatccgaa tcatatccct caagcttata
     7081 ttgataccga actaggcttg gtcttgctgt ttcaatggcg ccaaattcaa tagtaatatt
     7141 gttaaactga tagggtaaac tgagtttttt aggaattgga taaaaggccg aaatgtgctc
     7201 aaactcaatt ccgctgaatt tctttttgaa ttcttggcgc tcagcttctg ttaatactcg
     7261 tccaaaaacc aatacctctt catttcggta tgccggtaca aataacgaat tggtttgaga
     7321 agtatcctct cgtaagtcct gccaacaaat ccgttcatta tttacgcgaa tggcgtgtag
     7381 ttctaataaa cccgattttc ttccagtaat cggctgacga taatcaaacc gaactagccc
     7441 ttcggaagta ccagcccaca caactccacg acgatcaaaa tacatggagt ttggataaaa
     7501 atcttcaact ggaaatcccg tgcgtttatt atatatctcc acttctggct tccatccttt
     7561 ttgttggagc tcctgattcg tcaaactatt cgtggcagaa attcgcgatt caggagtatt
     7621 caaactatcc tgttccgaac gaaacatccg aatgacactg aaccctttgt tcgttcccaa
     7681 ccaaatactt ccatcttggg ctactttgct agctaagatt acatcatcac ccaaaccttg
     7741 agcaaccgat atcgacaaaa atgattttcc atcaaatcgg ctaactccac caccatcgct
     7801 ggcaaaccat aaattccccg catcgtcttg gttgatgttc caaatcacat tgccaggtaa
     7861 gccttgatcc atcgaataat tcagaaattc cttaccagta aaacaacttg ctcctcctcc
     7921 atgtgtcgaa aaccagatat tgcctttccg gtcttccata atattccaaa cttcattatt
     7981 cggcaagccc tgttcagttg tataagttaa gatcgaatct cccgcgatac agctaactcc
     8041 ttgtttaaaa ctaccaaccc aaattcgccc tttgctatct tcaaatacac ttcgagtatt
     8101 gtttccagca agcccttgac ctaccgtaaa actaaatatc gttttcccat caaaccgaca
     8161 aactccagca ccgcttgttg caaaccaaat acttccctta gagtcgtaaa tcgatcctcg
     8221 aacaactcca aaacgattta ataaggagtt tgaaaacgaa cgaatctgct ccccatcata
     8281 acgaattaaa ctttgtgttt ggttggtaaa ccataaatgc ccagccttgt cttctgccaa
     8341 actcaccact ttaatgtttt cctttccctc attggtcatt aaatgcgaaa ttgaatttcc
     8401 gctatactga acaactccgc cgccccaaga tccaaaccat aaatttcctg aatgatcttc
     8461 tgttatactg taaatctgat tgtttggtaa accatttcca gtagtcagat ttctaaacgc
     8521 ctttctaccc gaaagaggct ccaccgttgg gtcataacag ctcacaccac cgttttgggt
     8581 tccaatccaa atcatgcctt tatcgtcttc gaacaaacac aaaacactat tgtcggcaag
     8641 accatctact tgggtaaaat gatccatcct ttttccgttg aaccgacaaa gtccgctgtt
     8701 agttccgatc cagatttttc ccgatttgtc ttgtagcata caagtaaccg agctatcagc
     8761 taaacccaag cgcgcattta atgctaaaaa tggattcgga ttatttcctg aaggatcgta
     8821 cgcttgagcg ccagaagatt tactgccaat ccaaatcatc ccacgcttat cctcataaat
     8881 acactgaata aattctgaac gcgagtttat ttgtgtatta aatggctcta gcttagacga
     8941 atagccttta ttctgtggtt ttaacaaatt aactcccccg ccttctgttc caatccaaac
     9001 cactccattt tttcctatag caattgcacg aatatcactg ttggtaagcc cttctgaagt
     9061 gctgtaattt ctaaatcgca atccgtcgta gcaagaaatt cctccgcctc gggttccaaa
     9121 ccacatatta ccatgcttat cctccgctat gcaacgaact atattatctg ctaaaccttg
     9181 gttagctgta taattcacga aactatgtcc gttgtaacaa ctcactcctc ccccattcgt
     9241 tccaaaccac aaattgccgc gagaatcaca aaatccgcat ataactgcgt taattcccag
     9301 tccgtcttct gaactaaatt tcttcagcgg aacttcaaaa tttgctgaag cagctgctgt
     9361 ctcgatgccc tcgggatcga ataagtttcg gtttgaaaga atggcttgtt ctggagcagg
     9421 acaatttttc agatttacaa tcctcggttt tcctgcaaga actgtttgaa caggttttga
     9481 attgctcttt tctggcaaaa ttggtggccc tttccgctct tcaactgaag aacaggatac
     9541 taaaaagccc aacaataatg caatcagatt aatgcgcatg aagcaataca attcagttcc
     9601 agaaccagtt gtttgaattt cgacttttgc aaatatacac ggctcaactg caacccgcga
     9661 atttattaga aatagcaagc atttaaattt tttggggtcc tgcagaaaag ccatccgcta
     9721 cgctccggct ttcatgcacc gggctttcgc ttcgccaaat aggctctgct ctatccctgg
     9781 cccaatgttt tgtgtacctt tgcaaaatcc acttgatatt ttctaccatg attctctacg
     9841 gaataaaaaa ttgcaacacc atgcaaaaaa ccaccgatat gctcgatagc aaaggagtgg
     9901 attacacttt ccataannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncgcc
    10021 aacaaatacc acattggtta tcggtcgagt ggttgatgaa tcgggcaatc ctgtagcagg
    10081 atcttcggtt tctgctggtt ctatttcttc gattagcaat tccgacggaa ccttcgttct
    10141 ttccggagtt agcggaacat ccagagtatt tgtatcggct aataaatccg gatacttcaa
    10201 aggttcaaga ggagccattg ccgaaaataa cggcatcacc aaaattgaaa tcaccctact
    10261 cgaaaacaca cctaacttta ttgctaatgc gggttcttct aattcacttt tactaagcaa
    10321 tggctctggt gttgaattag cttcgaatac cgttcaagat caaagcggcg ctagcgtttc
    10381 agggtcgttt caagtttcca ttgttcacct cgatcctgca gatccagatt tcgaaaataa
    10441 agttcccggt ggtgatttaa ttggcgtgga tgcagcatct caagaactac aacttttttc
    10501 atacggtatg cttctagttc aaatgactga taatagcggc aatgagcttc aattagcatc
    10561 tggtagtacc gccacaattc gcatgaaagt gccaacatct caattatcaa cagctccatc
    10621 aagcattccg ctttggcact ttaacgaaac ttccggaaag tgggaagaac aaggttctgc
    10681 atcacttcaa ggcgatacat acgttggaac ggtttctcat ttctcttctt ggaattgtga
    10741 tcatcccggt tctcgcgcta cagttcgtgg cttagttcgc gactgtaacg gaaacgtact
    10801 tcctggcatt cgcgttcggg ttggacaaac atctgctttt accgatgcat ccggccaata
    10861 cgaaacattt gctcctgcgg gtgtcgattt cgatgttgct gttgaatctc ccgaactcgg
    10921 tattttttct acaccgaata ctgtttcaag cttaactgca ggttcggttt tcgatgctcc
    10981 tgcgataagc gttgcttgtc ctggttatgt aaccatgagc ctagcttgta gctccggttc
    11041 tactttcaac gcttccgttt cggtaaattg gggcgcttct caaagtgtat tcggctctac
    11101 tcaaactcca ggaacgttca aaattgctgt tcccactaac ggacaaacag caaacatcat
    11161 cgttacgagc ttaacgaatg gcgattctta ttcggtttct gtaacactgc caacatctgc
    11221 caatcaagag attagcgccg gaactttcga aatgtgtaat acgagctcta gtggaaatac
    11281 catacaatcg agctttacaa ttaatggtga tggttttaat aatcaaacga ttactattaa
    11341 tgctatgcct gtaatttgtt actctgtctt caatacatcc gatttttcga ccgtaggaat
    11401 tgcaatggaa acttatggct taacagttat ttttccggga aacactgtca atacctatga
    11461 acacaacgat tcaacagatg tttatgcaac cattacaaac aatggtaatt catgggtagc
    11521 cgattctgta ttaaccataa acgtgactga atttggtcct gttggcggtc gagtaaaggg
    11581 aaatttcaat ggtattttta gacgtgtgga ttataatcaa actagcggag aagtcaattt
    11641 cttttacacg acagtaacaa atggacattt tgaatttatc cgtcgcccag acgagaacta
    11701 atccgaaata gaataattca agcccgacat ggaattttct gtgtcgggtt ttttattgat
    11761 taaaacccat aggcaattta aattagctcg gctttgattt tacccacttg aataaacaga
    11821 tatatactac cgtcgagcct gaataattca tacttttgca ccacaaaatt cacctgctat
    11881 gtcaac
//
LOCUS       DAOOQW010000053        11838 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_6715_length_11838_cov_5.462022, whole genome shotgun sequence.
ACCESSION   DAOOQW010000053 DAOOQW010000000
VERSION     DAOOQW010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 11838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11838
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6715_length_11838_cov_5.462022"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(38..334)
                     /locus_tag="PK281_04800"
     CDS             complement(38..334)
                     /locus_tag="PK281_04800"
                     /inference="COORDINATES: protein motif:HMM:NF014479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muconolactone Delta-isomerase family protein"
                     /protein_id="HOY48697.1"
                     /translation="MNQYLVDLDLPIEMTEEFIALIPEQRRQINELMSGGIVLSYALS
                     QDRTKVWATINAESELEVQEILESMPLIDYMEPTIYELAFYNATGNGLPAISLN"
     gene            complement(429..764)
                     /locus_tag="PK281_04805"
     CDS             complement(429..764)
                     /locus_tag="PK281_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006265470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HOY48698.1"
                     /translation="MTKNRKQTPVKPARNPVFDGVCLASAYIMQDGKPVGFMYREEPE
                     EDLDSGWRFMSGDEDDAYLDNEDNYGVYDVEAVMLLDPAIKQYLKLPYGTELERKDDH
                     FVQLPPEEE"
     gene            complement(768..1259)
                     /locus_tag="PK281_04810"
     CDS             complement(768..1259)
                     /locus_tag="PK281_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48699.1"
                     /translation="MQEPEEFQELTDANFFTVAEVYQLTEMVNETLASVSYHYWVNRA
                     QGQLFEVLDWITFHFDSGRSLSITAGPDSDGIKVSEPNIEAERKRLEEEFKGVVTVET
                     RNASKIRFWKDAIGKSITPSFIKHEGKVLNDSVVLKFEGAEPVEIFLGLEGLEVDDFD
                     EED"
     gene            complement(1262..3370)
                     /locus_tag="PK281_04815"
     CDS             complement(1262..3370)
                     /locus_tag="PK281_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOY48700.1"
                     /translation="MKLIRIAGLLMLGLTSLPETQAQLTYPNTRKTDVVEDYHGTKVA
                     DPYRWLENDTSAETKAWVIAENEVTNSYLSKIPYRNKIRDRINELLNYPRYSAPFSAG
                     KFYIYSKNDGLQNQSVYYRQDGLTGEPTVFLDPNTLAKDGTASAGFAGVSGDKKYIAY
                     TVSRAGSDWSEMRIREVATGNDLPDVIQYVKFSGAAFVGNGFFYSRYPSPEKGQELSG
                     QNKFHSIYYHQLGDKQERDRLIFIDKANPLRYHSAYATEDENFLVLSISEGTDGNETW
                     IRDLKAGEESFHPLISGFDFVSNVVDNEGDKLLVHTNIDAPNYRLVLIDPKNPAKANW
                     KNIIPESANLLKGVSTCGGKFFAEYLKDVNSQVFQYDRTGKMEHEIALPGVGAAGGFG
                     GYKEDTFCFYTYTSFNYPPTIFKYDIASGKSTVFRKSEVKFNPEDYEVKQVFAPSKDG
                     AKVPMFIVHKKGLKLDGQNPTLLYAYGGFNVSLEPGFSATRIPFLENGGVYVMANLRG
                     GGEYGETWHRAGMMEKKQNVFDDFIACAEWLQANKYTSKEKLAIQGGSNGGLLIGAVM
                     TQRPDLFAACIPQVGVMDMLRFQKFTVGWGWVDEYGSSEDAKMFPILYGYSPLHNIKE
                     VSYPATMVMTADHDDRVVPAHSFKFIATLQEKNKGANPVLIRIETNAGHGAGTALSKS
                     VEQAADIYSFIFFATHSPVN"
     assembly_gap    3446..3545
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3835..4365)
                     /locus_tag="PK281_04820"
     CDS             complement(3835..4365)
                     /locus_tag="PK281_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48701.1"
                     /translation="MNWFETVFDLLKYTIPLIVVFGFIYFILNNYLEENFKLRSLDLK
                     LTNQNAITPVRMQAYERIVVYLERITPSNMIMRIHKAGMSSRLLHAELLKTVRGEYDH
                     NIAQQIYMSNASWELVKSAKEEMIKLINAAAHKVGENADGVELAKVLLDITMSVEKLP
                     TQIALEYVKKEIRQSF"
     gene            complement(4497..7049)
                     /locus_tag="PK281_04825"
     CDS             complement(4497..7049)
                     /locus_tag="PK281_04825"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOY48702.1"
                     /translation="MKKVKLHGFPEEEIRVQVDLERMAQLGIPLNRVLGAIQTEASNI
                     PGGSIDAGARKFNIKTSGDYGSIEEIRNTVIATSGEKIVHLRDLAEVQLEEGDQSYRA
                     RFNGKRAMFISITEKEHTNIIETYGKVMPVLNAFQQKLDPSIKLENGFVQAEDVSKRL
                     TGFSRDITIAIFLVLLTLLPLGTRASIVVMISIPLSIAIGLTALNLLGYTINQLSIVG
                     LVIALGLLVDDSIVVVENIERFLRNGYTRKEAAIAATKQISLAVLGCTATLIVAFLPI
                     LFLPEGSGDFIRSLPMAVITTVLASLLVSLTIVPFLSSKILKPHENPKGNIFLRGLQR
                     LISGSYRELLVKALKRPILTIILAVVVFVGSVMLFPVVGFSLFPKSEKPMFLVNVETP
                     LGTNLDATDSVAHDVEKELLQVKGVKAVFANVGKGNPRVYYNIAGKNESANTAQFFVR
                     MEEMTLSEIEERVEDLRNKFTGYPGARIEVKQFEQGPPIEAPVAIRVFGENLDTLRTL
                     AAQVEARMKQTDGTIYTHNPLETYKTDIRVNINRDKASLYGVQTSEIDRTVRMGIAGL
                     KVSSYKDASGDDYAINVTLSHDRTQTLEVFNKLQVSSLSGKLIPLRQLANLSFETSVP
                     TISHYNKNRYVTVTSFVKKGFNTMNVTNDLLKKMEGIKLPEGFSFQAAGEVESSQESF
                     GGLGTIILITIFGFLAILLLEFGTFKSTMIVLSVIPLGIIGAVLILLLTGNTLSFVAV
                     VGMIALAGIEVKNSILLVDYTNYLREQGMELNEAIMEAGETRFLPIILTTMTAIGGMI
                     PLVLENSPLYSPLAWVLIGGLISSTVLTRLVTPVVYKLLAPKVEVKSHGLID"
     gene            complement(7076..7555)
                     /locus_tag="PK281_04830"
     CDS             complement(7076..7555)
                     /locus_tag="PK281_04830"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOY48703.1"
                     /translation="MNISEFSVKNYQFTIVISLLLLGLGLSALFTMPRGEDPPFNAPL
                     YFIIAVYPGTSPNDMEKLVADPIEEKLNELNDIKRIRSTIDDGLLVTQVEFTYGSDID
                     SKYNEVVREVNGIRANLPADMLSLDIQKVTSSDVSTYQLALVSETVPYHILQDKANL"
     gene            complement(7575..8630)
                     /locus_tag="PK281_04835"
     CDS             complement(7575..8630)
                     /locus_tag="PK281_04835"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOY48704.1"
                     /translation="MLKYFLPLSSILVVFFAACSAKSEPEKAEKTAVNVEVQTFQSSL
                     LAVPVQASGIITSDAEARLSFKTGGLISRMLVQEGQQVQKGQLLASLQLTEINAQVNQ
                     AQQGLDKAKRDFNRVEALRRDSAVTLEQFQNAKTALSVAEETVQIARFNQQYSSIVSP
                     VSGKVIRKLMNEGELAGPGMPVYFIQSNAQADWVVKLGVADVDWTRLQVGDSASVFAD
                     AFPGEVIPAIVDELGFAADPMTGTFPVKLRLVAGNRSLASGMVAKMTIHPHAKEAHLV
                     LPMQCLSEAQEDRASVFVLSKDKKTVSRVEVKILQILPEGVVVTGVPENSSIVTKGAA
                     YLHDQSQVIVTSASAKN"
     gene            complement(8664..10007)
                     /locus_tag="PK281_04840"
     CDS             complement(8664..10007)
                     /locus_tag="PK281_04840"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOY48705.1"
                     /translation="MVNIKAFSFFILLSGIASAQPSSIDQLIKTGLESNHSLKQHQIQ
                     LDKANAALHEARGLFLPSISFQAEYTYAQGGRKLNFPIGDLLNPVYGTLNQLTASNAF
                     PQVENVDIQFLPNNFHDTRLRSSLPIVNAEIWYLNKIRQEAIQGQQAELMVYKRNVVR
                     DIKSAYANYLKTLQAISIYQSGVDLLEKNLKLTQALVKQDMALPAAVFQVKAELSATQ
                     SKLESMNNDSRNAAAYLNFLLNRDLATEIPNDTLWLKTAGNQPSFNSFATQREEILQL
                     QSGLKQTESLLSMKKAYYIPTLGTFFDAGYQGFGYKMNSDQRYYLGGFQLKWNLFAGR
                     QNTWKVKQANSDVLLLNDRISEAESQLRLHLTTSQNALASALKQLESSKEALTFARES
                     YRLTAIRYENGQAIPLELTQALQQLTQAQLTLAIQQSEVRIREAELERAAASFTF"
     gene            complement(10051..10353)
                     /locus_tag="PK281_04845"
     CDS             complement(10051..10353)
                     /locus_tag="PK281_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48706.1"
                     /translation="MHTSYIAHPENLQFTSPQEKLSFVHKEFSRLLYCHFTPHLHRSL
                     LDDRSFTLSELGIENEFCTLLGFIIENHFHIQLPTSLNTSQSISDIESIISQHFAN"
     gene            complement(10393..11001)
                     /locus_tag="PK281_04850"
     CDS             complement(10393..11001)
                     /locus_tag="PK281_04850"
                     /inference="COORDINATES: protein motif:HMM:NF024701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOY48707.1"
                     /translation="MGIAERKEREKTEMRDLILQAATELFVERGYDDTSIRTIAEKIE
                     YSPATIYLYFKDKPELFHAMMDHAFSLLIEKFSVVDKSAHPIEQLRQLGQVYMKYALE
                     NPALYDLMFIMRKPMDHVACAEDWDCGLQAYHFLYTIIETCMQQELVRFTDAHVGAIT
                     VWSHLHGLIALNLRGRFVMIPEEMHAPLIQASIDSIIEIIQN"
     gene            11281..>11838
                     /locus_tag="PK281_04855"
     CDS             11281..>11838
                     /locus_tag="PK281_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48708.1"
                     /translation="MQQLYTQLKKTAATFLFVLIACFTTLSATAQHSVSREWNEAMLQ
                     CIRKDLARPVVHARNIFHISASMYDAWAAFDTTASTFLLGKTVGTYTCTFNGIPAPTD
                     RVAAQEEAISFAAYRMIRARFLNSPGAAISLPPLDTLMISLGYDPSITSVDYSTGSPA
                     ALGNYIATSYIAYGLTDGANQTGNYA"
BASE COUNT         3411 a         2464 c         2405 g         3458 t
ORIGIN      
        1 ttcttccccg ctggctgagc ctgtcgaagc cagcgaatta gttcaacgaa atagccggta
       61 agccattacc agttgcgttg taaaatgcca attcgtaaat ggttggttcc atataatcaa
      121 tgagtggcat agattctaaa atctcctgca cttccaactc cgattcggca ttaatcgttg
      181 cccaaacctt ggtgcgatct tgcgataaag cataagacaa tacaattcct cccgacatta
      241 attcattgat ctgacgccgt tgttcgggaa tcagggcaat gaactcttcg gtcatctcta
      301 taggaagatc taggtcgacg aggtattgat tcattacaca atgataggga aaagcactat
      361 ttccacatca acaggataaa aaaaggcagg aatttaatgt tcctgcctat ctgtgtttta
      421 cgttttgatt attcttcttc tggtggtaac tgcacgaaat gatcatcttt ccgctcgagt
      481 tcggttccat aaggaagttt gaggtattgc ttgatagcag gatctaacag cataaccgct
      541 tccacatcat acacaccata attatcttca ttatcgagat aagcatcatc ttcatcgccc
      601 gacataaatc tccagcctga atccaaatct tcttcaggtt cttcccgata catgaatcct
      661 accggttttc catcttgcat gatatacgca cttgccaaac atactccatc aaaaacagga
      721 ttacgggcag gcttaacagg ggtttgcttt ctattctttg tcatagctta atcttcttcg
      781 tcaaaatcat ccacttccaa accttccaaa ccaaggaaga tttctactgg ttcggcacct
      841 tcaaacttaa gtaccacact atcattaagt acttttcctt catgcttgat aaacgagggt
      901 gtgatgcttt ttccaattgc atctttccaa aaacgaatct tggatgcatt gcgcgtttca
      961 accgtcacaa cacctttaaa ttcttcttct aagcgtttac gttcggcttc aatattgggc
     1021 tcgctcactt ttataccatc cgaatcggga ccagcagtga tggataaaga acgtccacta
     1081 tcaaaatgaa acgtaatcca atcgagcact tcaaataatt gcccttgagc acgatttacc
     1141 cagtaatgat aacttactga tgcaagtgtt tcgttcacca tttcggtgag ttgataaacc
     1201 tctgctacag tgaaaaaatt ggcgtcggtt aattcttgaa attcttcggg ttcttgcata
     1261 attagttgac aggtgaatgg gtagcgaaga agataaacga ataaatatca gcagcttgtt
     1321 ctaccgattt acttagtgct gttccagctc catgtcccgc attggtttca atacgaataa
     1381 gaactggatt agcaccttta tttttttctt gcaaagtagc tatgaatttg aaggaatgcg
     1441 ccggaacaac tcggtcgtcg tgatcggcag tcataaccat agtggcagga taactcactt
     1501 ctttgatgtt atgaagtgga gaataaccgt ataaaatcgg gaacatttta gcatcttctg
     1561 atgaaccata ttcatcaacc cagccccaac caactgtaaa tttctgaaag cgtaacatgt
     1621 ccatcacccc tacttgagga atacatgcag caaataaatc gggacgctga gtcataacag
     1681 caccaatcaa caatcctcca ttagaaccac cttgaattgc gagtttttct ttagatgtgt
     1741 atttgttggc ttgcaaccat tcggcacagg caataaaatc gtcaaataca ttctgctttt
     1801 tctccatcat tccggcacga tgccaagtct caccatactc tccacctcca cgtaaattgg
     1861 ccatcacata cacaccacca ttttctaaaa aaggaatgcg tgtagctgaa aatccaggtt
     1921 ctaaactcac attaaatcct ccgtatgcat atagcaaagt cggattttgt ccgtcgagtt
     1981 tcaaaccttt cttatgtacg ataaacatag gcacttttgc accgtcttta cttggagcaa
     2041 acacctgttt cacctcataa tcttcaggat tgaatttcac ttctgattta cggaaaacag
     2101 tcgatttacc agaagcaata tcatacttga aaattgttgg aggataattg aatgaagtat
     2161 aagtatagaa gcagaatgtg tcttctttat agccaccaaa tccacctgca gcaccaacac
     2221 ctggcagagc aatttcgtgc tccattttac ctgtccggtc gtattgaaaa acttgagaat
     2281 tcacatcttt caaatattct gcaaaaaatt tccccccgca ggtagaaaca cctttcaaca
     2341 aattagcact ttcgggaatg atgtttttcc aattggcttt tgccggattc ttaggatcaa
     2401 tcaataccaa gcggtaattc ggagcatcga tatttgtgtg aaccaataat ttatcacctt
     2461 cattatcgac tacattactt acgaaatcaa aaccgctgat aagtgggtga aatgattctt
     2521 cacccgcttt caaatcacga atccaggttt catttccatc ggttccttcg cttatagata
     2581 aaaccaagaa attctcatcc tctgttgcat aggcactatg ataacgtagt ggatttgctt
     2641 tatcgataaa gattaaacga tcgcgttctt gcttatcgcc caattggtga tagtaaatcg
     2701 agtggaattt attttgtccc gaaagttctt gacctttttc gggtgatgga taacgcgaat
     2761 aaaagaaccc atttcctacg aaagcagctc ccgaaaattt cacgtattga atcacatccg
     2821 gtaaatcgtt ccccgttgct acttcacgaa tgcgcatttc ggaccaatcg ctgcctgcac
     2881 gactaaccgt gtaagcaatg tattttttat cacccgaaac tcctgcaaat ccagccgaag
     2941 ctgtaccatc tttagccaag gtattcggat cgagaaaaac cgttggttcg ccagttaaac
     3001 catcttgacg atagtaaacg ctttggtttt gtaaaccatc gtttttgctg tagatgtaaa
     3061 acttcccagc actaaatggt gctgaataac gtggataatt gagtagttcg ttaattcgat
     3121 cacgaatctt gtttcgataa gggattttag acaaatatga attcgtcact tcgttttcgg
     3181 ctatcaccca tgctttggtt tcggcagaag tgtcgttttc taaccaacga tagggatctg
     3241 caaccttagt tccgtgatag tcttccacta catcggtttt ccgagtgtta ggataggtaa
     3301 gttgcgcctg agtttcaggc agagatgtaa gtccaagcat aagtagaccg gcaatacgaa
     3361 tcagtttcat attcagggat tgaagcgcga aaggtacttt ttaaaaatgc ggctacaaaa
     3421 tgcacgggtc aaaatccgtt gacctnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnaaggt actttttaaa aatgcggcta caaaatgcac gggtcaaaat ccgttgacct
     3601 caatccccca gccccttcct ccatcctata gggaccacta tgtggcagag aaaagggaga
     3661 aacatacatg tttgctggaa gattcagcta atcgttttct atcgaaatac aaagtccctc
     3721 tctgccacct agtggtccct tcgggagaga gggatttagg gtgaggtcta acaaaaatgc
     3781 tgcataaata gagtgtgatc tattcatgct gcattagctt ggaagtttga tttatcagaa
     3841 cgactgacga atctcctttt tcacgtattc caacgcaatt tgagtaggaa gtttttctac
     3901 gctcatggtg atgtcgagaa ggactttagc aagctcaact ccatcggcat tctcgcctac
     3961 tttgtgagca gcggcgttga tgagttttat catttcttct ttagcacttt tcacgagttc
     4021 ccaagaagca ttcgacatat aaatttgttg ggcgatgtta tgatcgtatt cgccacgaac
     4081 ggttttcagc aattcggcat gtagtaaacg ggaactcatt ccagctttgt gaatgcgcat
     4141 gatcatattg cttggagtga tgcgttcgag gtaaactaca atgcgctcat acgcctgcat
     4201 gcgaacagga gtgattgcat tttggtttgt gagcttgagg tcaagtgaac ggagtttgaa
     4261 attttcttcc aagtaattat tgaggatgaa atagataaat ccaaaaacaa caattagcgg
     4321 aatcgtgtat tttaaaagat caaaaacggt ttcgaaccag ttcatgtgcg gtttattttt
     4381 attggcgtaa ggtatgaaac gttacgaacc tgaaacaagt tgcatgaaaa aatggaggaa
     4441 tgcaattttt tattcagctc cattttttca atactcattt aaatccaaaa gcttatttaa
     4501 tctatcaaac catggctttt cacctccact ttaggagcaa gaagtttata tacaactggc
     4561 gtcaccaaac gtgttagcac agttgaactt atcaatccac caattaaaac ccaagccaag
     4621 ggtgaataca aaggtgaatt ttccaagacc aaaggaatca tcccgccaat ggcagtcata
     4681 gtagttagaa tgataggaag aaatcgtgtt tcaccagctt ccatgatggc ttcgttgagt
     4741 tccatgcctt gttctcgcag gtaattggtg taatcgacca acagaatgga gttcttcacc
     4801 tcaattcctg ccagagctat cattcccaca acagcaacaa atgaaagcgt attgccggta
     4861 agtaaaagaa tcagcaccgc tccaattata cctaacggaa taaccgacag cacaatcatc
     4921 gtactcttaa acgtaccgaa ctctaaaagc aaaatcgcta ggaaaccgaa aatcgtaata
     4981 aggattatcg ttcccaaacc accgaaactt tcttgtgaac tttctacctc acctgcagct
     5041 tgaaaactaa atccttcggg tagtttgatt ccttccatct tcttgagcaa atcgtttgtc
     5101 acattcatgg tgttgaagcc tttcttgaca aagctcgtca ccgttacata gcgattctta
     5161 ttgtagtgac tgatagttgg aacagatgtt tcgaaactca aattggctaa ttgtcgcaaa
     5221 ggaatgagct tacctgatag ggaagaaact tgtagcttat taaatacctc aagcgtttgg
     5281 gtgcggtcgt gcgataatgt aacgttaata gcataatcat cacctgaggc atctttgtag
     5341 gaactcactt taagtccggc aattcccata cgtaccgtgc gatcgatttc ggaagtttga
     5401 acaccataga gcgaagcttt gtcgcggttg atgttgacac gaatatcggt tttgtaggtt
     5461 tcgagtggat tgtgcgtgta gattgtccca tcggtttgct tcatacgcgc ttccacctgt
     5521 gcggcaagtg tgcgaagtgt gtcgaggttt tctccaaaaa cacgaatggc tacgggtgct
     5581 tcaataggcg gaccttgttc aaattgcttc acttcgattc gtgcacctgg atagccagtg
     5641 aatttattgc gcaaatcttc cactcgttct tcaatttcgg aaagtgtcat ttcttccatg
     5701 cggacaaaaa actgagccgt attagccgat tcatttttac cagcaatatt gtaatacact
     5761 cgtggatttc cttttcccac attggcgaaa acggctttca caccttttac ctgaagtagt
     5821 tctttttcca catcatgagc aaccgaatca gtagcatcta gattggtccc aagtggtgtt
     5881 tcgacgttca ctaaaaacat gggtttctcg gattttggaa aaaggctaaa tccaaccaca
     5941 gggaataaca tcacacttcc tacaaaaact actactgcta gaataatagt tagaattggg
     6001 cgtttcaatg ctttcactag caattcacga tacgaaccac tgatcaggcg ctgtaatcca
     6061 cggagaaata tattgccctt tggattttcg tgcggcttta ggattttact ggaaaggaaa
     6121 ggcacgatgg tcaaggaaac cagcaaggat gctagaactg ttgtgatgac agccatgggc
     6181 aagctgcgga tgaaatcacc cgatccttca ggtaaaaata aaatgggaag aaaagctaca
     6241 attaaggtag cagtacagcc taaaacagct agagaaattt gcttggttgc agcaatagca
     6301 gcttctttcc gggtgtaacc atttcgtagg aatcgttcga tattttcgac caccacaata
     6361 ctatcatcta ccaataaacc gagtgcgatt accaaaccaa caatgcttaa ttgattgatt
     6421 gtataaccga gaagattgag tgccgttaat ccgatggcga tagaaagtgg aatactaatc
     6481 atcaccacaa ttgaagcacg agttcctaaa ggaagtagcg tcaacaaaac cagaaaaatc
     6541 gcaatggtga tgtcgcgaga aaagccagtt aagcgtttcg acacatcttc ggcttgaaca
     6601 aatccatttt cgagtttaat ggatggatcg agtttttgtt gaaaggcatt caacacgggc
     6661 attacctttc cgtaggtttc gatgatgttc gtatgttcct tttcggtgat ggaaatgaac
     6721 attgcacgtt ttccattgaa acgagcacga tatgattgat cgccttcttc gagttgtact
     6781 tcagctaaat cgcgtaggtg gacgattttc tcacctgagg tagcaataac agtatttcga
     6841 atttcttcga tggagccata atctccactg gttttgatgt tgaattttcg tgctccagca
     6901 tcaatacttc caccgggaat attcgaagcc tccgtttgaa ttgcacctaa aacacgattc
     6961 aagggaattc ccaactgtgc catgcgttcc aaatcgacct gaacccgaat ttcttcttcc
     7021 ggaaaaccat gaagtttgac ctttttcaag gttttaatct tctctaaatc cttcttcaaa
     7081 gattcgcttt gtcttgaaga atatgatacg gaaccgtttc gctaaccaaa gccagctggt
     7141 atgtgctcac atccgaggaa gtcacttttt ggatgtctaa actgagcatg tcggctggta
     7201 aattggcgcg tataccgttc acttcccgaa ctacttcatt gtacttgcta tcaatgtcgg
     7261 aaccataggt aaattcaact tgcgtcacaa gcaaaccatc atctatggtt gagcggatac
     7321 gtttgatatc gttgagctcg ttgagttttt cttcgatagg atcggctacc agtttctcca
     7381 tatcgttcgg actggttccg ggatacacag caatgataaa atacaagggt gcattgaaag
     7441 gaggatcttc accccgtggc atggtaaata atgccgacaa gcccaatccg agcaatagca
     7501 aggaaatgac aatggtgaac tggtaattct taacggaaaa ttcagagata ttcatcgctt
     7561 cagatggtat aaaatcagtt tttagctgag gcggatgtga caatcacttg actttgatca
     7621 tgcaggtaag ctgcaccttt ggtaacaatg ctcgaatttt cgggaacacc tgtcaccaca
     7681 acaccttcgg gtagtatttg aagaattttc acttctaccc tactcaccgt tttcttatcc
     7741 ttacttaata caaaaactga agcgcgatct tcttgtgctt ccgataaaca ctgcatgggc
     7801 aacacgagat gtgcttcttt cgcatgtgga tgaatagtca ttttagccac catgccactt
     7861 gcgagtgaac gattccctgc tactaggcga agttttactg ggaaagtacc ggtcatggga
     7921 tcagctgcaa accccaattc atctactatt gcaggaatga cttcacccgg aaaagcatcg
     7981 gcaaaaacag aagctgaatc acctacctga agccgagtcc aatctacatc agccacacct
     8041 aatttcacta cccaatcagc ctgagcgttt gattggataa agtaaacagg cattccgggg
     8101 ccagccaatt caccttcgtt catcagttta cgaatcactt tccctgaaac tggcgaaaca
     8161 atggaagaat attgttgatt gaaacgagca atttgtaccg tttcttctgc gacagataga
     8221 gctgtttttg cgttttggaa ttgttccaat gttacagcac tatcgcgacg caaagcttct
     8281 acccgattaa aatcgcgctt ggctttatcg agaccttgtt gtgcttgatt tacctgtgca
     8341 ttgatttcgg ttagttgcaa gctagctaag agttggcctt tttgcacttg ttgtccttct
     8401 tgcacaagca tccgggaaat gagcccgcct gttttgaagg ataaacgcgc ttcggcatcg
     8461 gaagtaatga ttccacttgc ctgaacggga acggctaata agctcgattg aaaggtttga
     8521 acctccacat ttacggctgt tttttcagct ttttctggtt ccgatttcgc ggagcatgcg
     8581 gcaaaaaaca cgactaaaat agagctgaga ggaaggaaat atttaagcat aattgaaaat
     8641 ataaaaggtt gggatagaag cgattaaaaa gtaaaactag cagcagcgcg ttcgagttcg
     8701 gcttcgcgga ttcgtacttc cgattgctga atggccagtg ttaattgagc ttgtgtgagt
     8761 tgttgtaaag cttgagtgag ttcgagtgga atggcttgtc cgttttcgta acgaatggca
     8821 gttagtcgat agctttcgcg ggcaaacgtg agtgcttctt tactgctttc gagttgtttg
     8881 agagcagatg caagtgcatt ttgagatgta gttaaatgca gacgtaattg actttcggct
     8941 tccgaaattc ggtcattaag tagtaataca tcggaatttg cttgcttgac tttccaggta
     9001 ttttgtcgac ctgcaaaaag gttccattta agttgaaaac cacccaagta atagcgttgg
     9061 tcggaattca tcttatatcc gaagccttga tatccagcat cgaagaaagt tcccagtgtt
     9121 gggatgtaat aggctttctt catgctgagc agcgattcgg tttgtttgag tcccgattga
     9181 agttgcaaga tttcttcgcg ttgggtagca aatgagttga aagagggttg attaccagcc
     9241 gttttcagcc aaagagtatc gtttggaatt tcggtagcta aatcacgatt aagcaggaaa
     9301 ttgagataag ccgcagcatt tcgactatcg ttattcatgc tttcgagttt ggattgcgta
     9361 gcgctgagtt cggctttcac ttgaaaaacg gcagctggca gtgccatatc ttgtttaaca
     9421 agtgcttgtg tgagtttgag atttttctct aataaatcaa ctcctgattg gtaaatagag
     9481 attgcttgaa gcgttttcaa gtagttggcg taggccgatt tgatgtcacg aaccacattc
     9541 cgcttataaa ccatgagttc ggcttgctga ccttgaatgg cttcctggcg gattttgttc
     9601 agataccaga tttcggcatt tacgatgggt aaagatgagc gcaaacgagt atcgtggaaa
     9661 ttgttgggaa gaaactgaat atccacattt tcaacctgag ggaaagcatt cgatgctgtg
     9721 agttgattca acgttccata aaccggattg agtagatcac caatgggaaa attgagtttt
     9781 cttccacctt gagcatatgt gtattcggct tggaaactta tagaaggcaa gaaaagtcca
     9841 cgtgcttcgt gaagcgcagc attggcttta tccagttgaa tttggtgttg cttcaaactg
     9901 tgattgcttt cgagacccgt tttaatgagt tggtcgatcg aagaaggttg agctgaggct
     9961 attccactga gcaaaatgaa aaagctgaaa gccttgatat taaccataga ttatacaata
    10021 aacatcgttt agtaaaaggg caaaaaaata tcagttagcg aaatgctggc tgatgattga
    10081 ttcgatatca gagatagatt gtgaggtatt caaacttgtc ggaagttgaa tgtgaaaatg
    10141 gttttcgatg ataaaaccga gcaatgtgca gaattcattt tcgataccta attccgacag
    10201 ggtaaaacta cggtcgtcga gtaaagagcg gtgcagatga ggagtaaaat ggcagtagag
    10261 taatcggcta aactccttat gaacaaagga aagtttctct tgaggagagg tgaattgtaa
    10321 attctcggga tgagctatat aagaggtgtg catcgtatga acagcgttta gttctagggt
    10381 aaaaaaaggt gattagtttt ggatgatttc gataattgaa tcgatagatg cctgaatgag
    10441 cggagcatgc atttcttccg gtatcattac aaatcgtcct cgtagattga gtgcgatgag
    10501 gccatgcaaa tgactccaaa ccgtgatagc acccacatgc gcatcggtaa atcgaaccaa
    10561 ttcttgttgc atacaggttt ctataatggt gtagaggaaa tgataagctt gcaatccaca
    10621 atcccaatct tcagcacaag caacatgatc catgggttta cgcatgataa acatgagatc
    10681 gtacaaagca ggattctcca atgcgtactt catgtacact tgtcctaact ggcgtaattg
    10741 ctcaattgga tgtgccgact tatctacaac agaaaatttc tcaatgagca aagaaaacgc
    10801 atgatccatc atggcatgga acagttcggg cttgtcttta aaatagagat aaatcgtagc
    10861 cggactatat tctatttttt cggcaatggt tcgaatggaa gtgtcgtcat atccacgttc
    10921 cacgaataat tcggtggcag cctgaagaat cagatcgcgc atttctgttt tctctcgctc
    10981 ttttctttct gcaattccca taatcaacta ctagattttg aacgcaacaa atatactaaa
    11041 cactgtttag taaaatcaaa aaccttagaa attaaacttt aaaaacctcc atctttttga
    11101 tttttaacaa tttataatct cggagaaata acagctacac ttcagcatta tatacatgtg
    11161 taggaatttt aggggtaatt ctatataaaa aattacattt taacagaatt tcttacttgc
    11221 tatttaaaac acaaccctgt acatttcaaa ttcttagtac ccactaaaaa caaaatgcct
    11281 atgcaacagc tctacacaca actaaaaaaa acggcagcaa catttctatt cgtattgata
    11341 gcatgcttta caactttatc ggcaacagca caacatagtg tgtcccgaga gtggaatgaa
    11401 gctatgttac agtgtattcg aaaagatttg gctcgacctg tcgtgcatgc ccgaaacata
    11461 ttccatattt cagcatcgat gtatgatgca tgggctgctt ttgatacaac tgcttctacc
    11521 tttctgttag gaaaaacagt tggaacatat acatgtacct ttaatggtat tccagctcca
    11581 acagatcgag tagctgccca agaagaagct ataagctttg cagcttaccg tatgattaga
    11641 gcgcgttttt tgaattcccc aggtgctgct atctcattac ctcctctcga taccttgatg
    11701 atttctttgg gatatgaccc atcgattaca tcagttgatt attcgactgg ttcgcctgcg
    11761 gcattgggaa attacatagc aacatcatac attgcttatg ggctaacaga tggagccaat
    11821 caaacaggga attatgct
//
LOCUS       DAOOQW010000054        11524 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_6987_length_11524_cov_5.101491, whole genome shotgun sequence.
ACCESSION   DAOOQW010000054 DAOOQW010000000
VERSION     DAOOQW010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 11524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11524
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6987_length_11524_cov_5.101491"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="PK281_04860"
     CDS             complement(<1..569)
                     /locus_tag="PK281_04860"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOY48709.1"
                     /translation="MAKPRDEQKIERIHAATLKLVQQQGFGGLKMADVAKEAQVATGT
                     LYVYFKDKVELINALYLHLKRKQAKHYLKPEDLDRPFKMSFRLVWENFLLVSLDFPEE
                     SAFIEQYYRSPFLRPEVRNEADLLLQAAHDVLLRGQSEELIKPLDTSLLLAHLSGSIL
                     ELVKWHYAGIIQLDRTNLDAPFSLAWDSV"
     gene            817..1737
                     /locus_tag="PK281_04865"
     CDS             817..1737
                     /locus_tag="PK281_04865"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOY48710.1"
                     /translation="MNRSFLYYLLISFGVCWLGAALIPLLDVGYGSFASTLIVALICM
                     PAPAFAGWLVQTRILKRPFSELGLTWKLANRSQLYLLPIWLFLLLIFNFVWIALLGNA
                     FGIEAFGTVDFSMDGFLLKLEEMTQGKTDLSEINFPSTYVIFLLVAMSGVVAGATVNL
                     PFTLGEEIGWRGFLFKQLENQSWLKRSLITGIIWGLWHTPLILMGHNYPEHPYLGVLM
                     MVFFCVSLSFPMDWVRRKTNSILGPGAIHGMINASAAGTFLFIQDGDSLMGSIAGFSG
                     ALACLTVFGAQYLVTKIIKEKESIAVHLPE"
     gene            1734..2768
                     /locus_tag="PK281_04870"
     CDS             1734..2768
                     /locus_tag="PK281_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015481911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48711.1"
                     /translation="MKTLSILFSAVLVSSSLLAQEPHLQNIRQLTFGGDNAEAYFSFD
                     NSKLTMQITNPKQGIPCDQIYLMDLQNQDKIVPRLISTGKGRTTCSYFMPGDKTILYA
                     STHLAADSCPPPPAPRKDGKYLWPLYNSFDIFVADLNGKIVKQLTDAPGYDAEATVSP
                     DGKMIVFTSDRSGDLELYIMDIDGKNVRQITSGLGYDGGAFFSPDSKKLVYRASRPQT
                     PEDIASYKQLLSEGLVAPTNMEIYTCNVDGTEQKQLTQLGKANWAPFFHPSGKKIIFA
                     SNHASTKGYDFNLFMINLDGSGLEQITNISNFNAFPMFSPDGKKLVFSSNRNNGGTRD
                     TNVFLADWKD"
     gene            2787..4013
                     /locus_tag="PK281_04875"
     CDS             2787..4013
                     /locus_tag="PK281_04875"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOY48712.1"
                     /translation="MKLRSIVLITVLFSSQLGAQNWFDPKLIVKHISVLASDSLRGRG
                     TGTEDERKAADYVISQWKAAGIQPKGEKDSWFQEFEFNAGMHGKGGRSGKTNNIIGFL
                     DNKAATTIIIGAHMDHLGDGSDGHSLDAHAKGQIHNGADDNASGTTGVIELARFYGMN
                     NETEKFNFLFICFSGEELGLLGSEYYANHPTIDLAQVNCMINMDMIGRLKTDKPVLEV
                     SGVGTAAEWMDMVKSFSSAAMEIKCDSAGVGPSDHTSFYNKQIPVLHFFTGTHSDYHK
                     PSDDVEKINAQGEEAVLMVISGVIAKLPTDHKLAFLKTRNPSMGSTSAFKVTLGIMPS
                     YAEDTAGLKVEAVLDDKAAFKAGMKDGDIITQMGDIKVTGIQSYMEGLGKFSKGDKTK
                     VTVLRGKETLVLDVEF"
     gene            complement(4099..4518)
                     /locus_tag="PK281_04880"
     CDS             complement(4099..4518)
                     /locus_tag="PK281_04880"
                     /inference="COORDINATES: protein motif:HMM:NF025887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HOY48713.1"
                     /translation="MKRIIFLLFLLPLGVQAQSEAQLAIRKVLDQQAADWNHGNLEAF
                     MQGYWNNDSLRFIGKKGITYGWKPVLENYRKSYSNQALMGVLTFDKLKIELLGEDAAL
                     VTGSWRVVTKTSEQGGWYSLIFKYIDNRWVIVLDHTS"
     gene            4569..5816
                     /locus_tag="PK281_04885"
     CDS             4569..5816
                     /locus_tag="PK281_04885"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOY48714.1"
                     /translation="MTNQEHSGTAALKVKPFRNFIAARFFLTMAIQMQAVAVGWQVYQ
                     LTKDPFALGIIGISEAIPFISLVLFAGYWADIYKRKQIILRSVTLFSLCAALLLFFAV
                     KPNLLPQNNPQWIFYLVIGITGIARALAGPAVQSMLPQLFKRELYANGAAWNSTVWQI
                     ASVSGPAVGGLIYGFYGKEVTYTVCMILVVLSLFFFSRLPDFPASNSKREGIFISLKQ
                     GLNFVFKQKIVLSALTLDLFAVLFGGAVSMLPVFANDILHTGAEGLGILRAAPSIGAV
                     ITSIYITRHAPMRHSGKILLTFVALFGLSMIAFALSEIFWLSFVILLLSGAFDAVSVL
                     IRSTIMQLFTPDEMRGRVSSVNSVFIGSSNELGAFESGLAAKTFGLVYSVIIGGCMTL
                     GVVATALRFAPKLRKLHLEDHVS"
     gene            5859..6518
                     /locus_tag="PK281_04890"
     CDS             5859..6518
                     /locus_tag="PK281_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011711049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HOY48715.1"
                     /translation="MITSFTEENYLKAIFKLAGQTEGNVSTNAIAEALQTKASSVTDM
                     MKKLGEKNLVNHTRYQGVKLTEAGKMCALDIVRKHRLWEVFLVERLGLGWEEVHDMAE
                     QLEHTSSPKLYEYLDRYLNYPRFDPHGDPIPDHTGKMERRSQISLLDVEAGVTHVVEG
                     VTDHLPDFLQYLQAINLMPGSTVTVTRRFAFDGSAGIRIGDSHEVIISRHVAAHILVK
                     S"
     gene            6522..6989
                     /gene="smpB"
                     /locus_tag="PK281_04895"
     CDS             6522..6989
                     /gene="smpB"
                     /locus_tag="PK281_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006990004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HOY48716.1"
                     /translation="MAQPNKQVTQLNIKNRKASFEYQLLMQFSAGIMLTGTEIKSVRA
                     GKVNIADAYCTFVKDELYVRNMHIQEYEMGNIFNHEPKRDRKLLLNKTELSKLRGKMK
                     DKGLTIVPLQMFLSESGFAKLDIALAKGKKLYDKRESLKDKDVKRSMARQDAD"
     gene            complement(6986..7630)
                     /locus_tag="PK281_04900"
     CDS             complement(6986..7630)
                     /locus_tag="PK281_04900"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HOY48717.1"
                     /translation="MPDSYRLKGLRNRLVDELRAKGIMSEPVLDAIRAVPRHQFFQDL
                     AFMEKAYEDIAFQIGAGQTISHPSTVAFQTQLLELKSGEKVLEIGTGSGYQTAVLAQF
                     GVKIFSIERQHALYIRTKKHLELMNISARLFFGDGYLGQPAFAPFDKILVTAGAPFIP
                     QALTEQLKPGGRLVIPVGVDGKQVMHVIDKQLDGTLQDAEHGEFRFVPLLESKS"
     gene            7814..8800
                     /locus_tag="PK281_04905"
     CDS             7814..8800
                     /locus_tag="PK281_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOY48718.1"
                     /translation="MLKIGVVGAGHLGKIHLKLIGEIDSYHLAGFFDADPEVAASVAK
                     DMNIHAFSTFEELLDAVDVVDVVTPTIYHHQYASKALRSSKHVFIEKPIATTVEEARD
                     LEQLAVEAGKKVQIGHVERFNPAYVAAMPFCQNPMFIETHRLALFNPRGTDVSVVLDL
                     MIHDIDIVLHSVNSTVKKISASGVSVVSETPDIANARIEFDNGCVANLTASRLSMKNM
                     RKTRFFQRDAYISVDFLDKEVEVIRMSTVEGEPDPLALTIELGEGKGLKQIFLEKPEI
                     LPTNAIKAELESFADAISHNQIPAVTATHGLQALDVAYRIIEKMNTTPHLFA"
     gene            8803..9669
                     /locus_tag="PK281_04910"
     CDS             8803..9669
                     /locus_tag="PK281_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48719.1"
                     /translation="MKYGYLIYLFICLSLFAGCDIINPEEQEAAYISIQQVNLSTDLD
                     IQGSNSSNIKDAWIYIDDNLIGAFPLPCKIPSLKTGNHKISIGAGIQVNGSSMLRTPY
                     IFYRFYQQENVNLEAGYVTELNPVVTYFDSITFGFKANFDDLSGNKLTSTAVSDTTVE
                     LTTDASKVFEGTGSCLARLLRDSGLVEFQMIDPVTLPKGGRNIFLELDYKTTHDLVIG
                     LRSYYTGASTKEEILLSLRANSNWNKVYINLTKAVSTQVNAANYRIYFSAYKQAGSVP
                     LEILLDNLKIVY"
     gene            9673..11100
                     /locus_tag="PK281_04915"
     CDS             9673..11100
                     /locus_tag="PK281_04915"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOY48720.1"
                     /translation="MISTKRDKIQGRIQVAADYFSAVLGWTLYYAFRKTYIEPIVFGH
                     PVRMEFNNTFWLGLAIIPVFWLILHHISGAYFDVYRKSRLGILGTTVWVSLLGSLILF
                     FAVSLDDFILSYRSYYISFSALFGIQFGVTAIQRMLIATRSARRIQSGKVYFNTLLIG
                     SGKKALDIYKKIEEQPVSSGNRFLGYVPVNGTLDPELSNTLQRIGHFDQMVQLIQDLD
                     VEEVIVAPENSEHAYLGKFLTELEVTTVVIKVIPDITDILLGSVRMSAIFGAPVIEIK
                     RQLMPRWQKSFKRLFDITVSLFVLVCLFPVYLITAIIVKTTSSGPALYSHPRIGFNGK
                     PFRIYKFRSMYCDAEKRGPQLSSKNDSRITPFGKFMRKTRLDEIPQFYNVLVGTMSIV
                     GPRPERQFFIDQIVEVAPHYRLLHKVRPGITGWGQVKFGYAENVDEMVERLKYDILYI
                     ENMTLALDLKILIYTFLIVLQGRGK"
     gene            11188..>11524
                     /locus_tag="PK281_04920"
     CDS             11188..>11524
                     /locus_tag="PK281_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009780018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HOY48721.1"
                     /translation="MKKIAVIGAGTMGNGIAHTFAQFGFPVILVDVSDAALSKGIQAI
                     TQNLDRQVQKGILTEAQKTETLTRISTQTQIDERLTDRDLVVEAASENFQIKSSVFKQ
                     LDQYCQPSCI"
BASE COUNT         3288 a         2397 c         2339 g         3500 t
ORIGIN      
        1 tttacactat cccaagccaa ggaaaatggt gcatctaaat tggttcggtc aagctgaata
       61 atgcctgcat aatgccactt gaccaactca agaatactgc ccgacaaatg tgccaatagt
      121 aaacttgtat cgagtggttt gatgagctct tcggattgtc cgcgtagtaa aacatcatga
      181 gctgcttgca gtaaaagatc tgcttcattt cgtacttcgg gacgcaaaaa tggtgatcga
      241 taatactgct caataaaagc agattcctca ggaaaatcga gactgaccaa taaaaaattc
      301 tcccaaacca atcggaaact catcttaaaa ggtcgatcta aatcttcggg tttgagataa
      361 tgtttggctt gtttgcgttt aagatgcagg taaagtgcat taatcaactc cactttatct
      421 ttaaaataca cgtacaatgt tccggttgcc acttgagctt ccttagccac atcggccatc
      481 ttcaaaccac cgaaaccctg ctgctgcacc aattttaagg tggctgcatg gatgcgttca
      541 atcttttgct cgtcgcgtgg ttttgccata atcgttgagg atgcaaatat aaatgaatat
      601 atattcaccg aacaaggttt tctttttttt tttcaaaagt tacatccatt gttaagtatc
      661 gcctgaggga ttgaagcgga aatcttttgt gaggcacgaa caaaaattgc agcgtaaagc
      721 ccgacccgaa atagttctac gaagcatcgc gaagtagaac gtatgtaggg acaggcccaa
      781 gcccaattgg aaatttcaat ttcctatctt gctcccatga atcgttcgtt cctgtattat
      841 ctgttgatta gctttggtgt ttgctggctg ggtgctgctc tcattccttt gctagatgtg
      901 ggctatggct cttttgcatc tacgcttatt gttgctctca tttgcatgcc cgctcctgcc
      961 tttgccggtt ggctggttca aacgcgtatc ctcaaacgtc ctttttctga acttggttta
     1021 acctggaaac tagccaatcg aagtcaattg tatttgcttc ccatttggct atttcttttg
     1081 ctgattttca actttgtttg gattgctctt ttgggtaatg catttgggat tgaagccttt
     1141 ggtaccgttg attttagcat ggatggattt ctgctcaaac tcgaagaaat gacacaaggg
     1201 aaaaccgatt tgagtgaaat taatttccca tctacctacg tgattttttt gttggttgct
     1261 atgtcgggtg ttgtggctgg tgcaacagtt aatcttcctt ttactttggg ggaagaaatt
     1321 ggctggcgcg gatttttgtt taaacaactt gaaaatcagt cttggctgaa acgtagctta
     1381 attaccggaa tcatttgggg attatggcac actcctctta ttctcatggg acataattat
     1441 cctgagcatc cttatttagg tgttttgatg atggtatttt tctgtgtttc actctcgttt
     1501 ccaatggatt gggtaagacg caaaacgaat tctattctag gaccgggtgc tatacatgga
     1561 atgattaacg cttccgctgc cggaacattt ttatttattc aagatggaga ttctctgatg
     1621 ggttccattg ctggatttag cggtgcatta gcctgtttga ccgtttttgg agctcaatat
     1681 ttagtgacaa aaatcataaa agaaaaagaa tcaatcgctg tacatttgcc cgaatgaaaa
     1741 cgctttccat tctattcagc gctgttctag ttagcagctc gcttttggct caagaacctc
     1801 atcttcagaa tattcgtcag cttacttttg gtggcgataa tgccgaagcc tatttcagct
     1861 ttgataatag caaactcacc atgcaaatca cgaatcccaa acaaggaatt ccgtgtgatc
     1921 agatttattt gatggatttg caaaatcaag ataaaatcgt tcctcgcctt atcagcaccg
     1981 gaaaaggtcg tactacttgc tcctatttta tgccgggtga taaaacgatt ttgtacgcat
     2041 ctacgcattt agctgccgat tcttgtccgc cgccaccagc accgcgtaaa gatggaaaat
     2101 acctgtggcc cttatacaat agcttcgaca tattcgtagc cgatttgaat ggtaaaattg
     2161 tcaaacaatt gaccgatgcg cccggatatg atgcagaagc aactgtttct ccggatggga
     2221 aaatgattgt ttttacttct gatcgttcag gtgatttgga attgtacatc atggatattg
     2281 atggcaaaaa cgttcgccaa ataacatctg gtttaggcta cgacggcggt gcattctttt
     2341 cgcccgatag taagaaattg gtgtatcgtg cttctcgtcc gcaaacaccc gaagacattg
     2401 ctagttataa gcaattactt agcgaaggat tggttgctcc aaccaacatg gaaatctata
     2461 cctgtaatgt ggatggaaca gagcaaaaac aacttaccca actaggaaaa gcgaattggg
     2521 cacctttctt ccatccttct ggtaagaaaa tcatttttgc ttctaatcac gcatcgacaa
     2581 aaggctacga cttcaatcta ttcatgatca atctcgatgg ttctggatta gagcaaatta
     2641 ccaacatcag caactttaat gcgtttccga tgttttcacc cgatggaaag aaactcgtat
     2701 tttcttcgaa tcgcaacaat ggcggcacgc gcgacaccaa cgtgttttta gccgattgga
     2761 aagattaata ccttattgaa accaatatga aacttcgttc gattgtcctg attaccgttt
     2821 tgtttagcag ccaacttggc gcacaaaatt ggtttgatcc taaactaatc gttaagcata
     2881 tttctgtctt agcttctgat tcacttcgtg gacgcggaac tggaaccgaa gatgagcgta
     2941 aagcagccga ttatgtgatt tcacaatgga aagctgcagg tatccagcca aaaggtgaaa
     3001 aggattcttg gtttcaagaa tttgaattca atgcgggtat gcatggtaaa ggtggacgta
     3061 gtggaaaaac aaataatatc ataggtttcc tggataataa agctgctaca accatcatta
     3121 ttggtgctca catggatcat ttaggtgatg gttcggatgg tcattctttg gatgcacatg
     3181 ctaaaggaca aatccacaat ggtgcggatg acaatgcttc tggaacaaca ggagtgattg
     3241 aattagctcg cttttatgga atgaataatg aaaccgaaaa attcaatttt ctattcattt
     3301 gtttttcggg cgaagagttg ggattattgg gttcggaata ttatgcaaat cacccaacaa
     3361 tcgatttagc tcaggtgaat tgcatgatca atatggatat gattggtcgt ttgaaaaccg
     3421 ataaaccagt attggaagtt tcgggtgttg gaacggctgc tgagtggatg gatatggtga
     3481 agtcgttttc gagtgcagct atggaaatta aatgcgattc ggcaggtgtt ggtccatcgg
     3541 atcatacatc attctacaac aagcaaattc cagtattgca tttctttaca ggcacacaca
     3601 gcgattatca taaaccatcg gatgatgtag aaaagatcaa tgcacaaggt gaagaagccg
     3661 ttttaatggt gatttctggt gtgattgcca aattacctac tgatcacaaa ttggcattcc
     3721 ttaaaacccg caatccatcg atgggtagca catctgcctt taaagtaaca ctaggaatta
     3781 tgccttccta tgccgaagat acagcaggtt taaaagttga agctgtcctc gacgataaag
     3841 cagctttcaa agcaggaatg aaagatggtg atatcatcac acaaatgggc gatatcaaag
     3901 taactggcat tcaatcctac atggaaggat taggaaaatt cagtaaaggc gataaaacca
     3961 aagtaaccgt attgcgcggc aaagaaacgc tggtgctgga tgtagaattt tagtcagcta
     4021 gaattattag ttaaattaaa tttcaaacga ggtgatttga atattcatgt gatcggaata
     4081 cggtcccaat tggatatttt aactcgtatg atctaacaca atcacccaac ggttatcgat
     4141 atatttaaat atcaaactat accatccacc ttgctcgctt gtcttggtta ccacacgcca
     4201 agaaccagtc actaaagcag catcttcgcc caataattct atcttcaatt tatcaaaagt
     4261 gagtacgccc atcaacgctt gattactata tgacttgcga taattttcca acacaggttt
     4321 ccaaccataa gtaattcctt tcttcccaat aaatcgcaaa ctatcgttgt tccaatagcc
     4381 ttgcataaaa gcttctaaat tgccatggtt ccaatctgct gcttgttgat ctaatacttt
     4441 acgaatagct aattgtgctt ccgattgagc ttgaactcct agtggcaata ggaacaacaa
     4501 aaaaataata cgtttcattt tcagattcat ttggtaggat gcaacgaagg tcggactttt
     4561 gtcgctaaat gaccaatcaa gaacattcgg gcactgctgc gttaaaggta aaaccctttc
     4621 ggaattttat agctgctcgt tttttcctca ccatggcgat acaaatgcaa gctgtggctg
     4681 ttggctggca agtgtatcaa ttaactaaag acccgtttgc tcttggaata atcggaattt
     4741 ctgaggctat tccgtttatt tcacttgtcc tttttgctgg ttattgggct gatatttata
     4801 aacgtaaaca aatcatttta cgctctgtca ccctgttttc cttatgtgct gctctgctcc
     4861 tattcttcgc agttaaaccg aatctgctcc cacagaataa tccacagtgg atattctacc
     4921 tcgttattgg cattaccgga attgcccgag cacttgctgg acctgccgtt caatcgatgt
     4981 taccgcaatt gttcaaacgg gaactttatg caaacggcgc cgcctggaat agtaccgtat
     5041 ggcaaattgc ttccgtttcg ggacctgctg ttggtggatt aatctatggt ttttacggaa
     5101 aagaagtcac ctacacagta tgcatgatac tcgttgtact ttcacttttc ttcttcagcc
     5161 gtttgcctga tttccctgca tcaaactcca aacgtgaagg aatcttcatc agcctgaaac
     5221 aaggtttaaa tttcgttttc aaacagaaaa ttgtcctttc tgcactaaca ctcgatttgt
     5281 ttgctgtact gtttggtggc gctgtttcga tgttgcccgt ttttgctaac gatatactac
     5341 acactggtgc tgaaggctta ggtattttac gtgccgctcc ttccattggt gctgttatca
     5401 cttcaattta catcacgcgt cacgccccca tgcgacattc gggtaaaatc cttctaacgt
     5461 ttgttgcctt gttcggtttg tcgatgattg cttttgcgct ttccgaaatc ttctggcttt
     5521 cgtttgtcat tctattatta agtggagcat tcgatgctgt gagtgttctc attcgttcta
     5581 ctatcatgca attgttcacg cccgatgaaa tgcgtggtcg ggtttcgtcg gtcaacagcg
     5641 ttttcattgg atcttcgaac gaattaggtg cattcgaatc gggattagct gccaaaacct
     5701 ttggtctcgt ttattcggtc atcattggtg gttgtatgac actcggcgtt gtcgccacag
     5761 cacttcgttt tgcacccaaa cttcgaaagc tgcatttgga agatcacgtt tcctaatcct
     5821 gcttcaaaca atctctttac aaagtctatc tttgtcgcat gattacctcc ttcaccgaag
     5881 aaaactacct taaggcaata ttcaagctgg ccggacaaac cgaagggaat gtgagtacca
     5941 acgccattgc agaagccttg caaacgaaag catcatcggt taccgatatg atgaagaagt
     6001 tgggtgagaa gaatttggtg aatcatacaa gatatcaagg tgttaagctt accgaagctg
     6061 gtaaaatgtg tgcgctcgat atcgtacgta aacaccgttt atgggaagta tttctggttg
     6121 aacgccttgg attaggttgg gaagaagtgc acgatatggc cgaacaactc gaacatacaa
     6181 gctctcctaa attatatgaa tacctcgatc gctacttgaa ttatccgcgt ttcgatccac
     6241 atggcgatcc tattcccgat catactggta aaatggaacg tcgctctcaa attagcttac
     6301 tcgatgtaga agctggtgtg actcacgtag tggaaggcgt taccgatcac ttacccgatt
     6361 ttttacaata tctgcaagca atcaacctta tgcccggctc aacagtaacc gttactcgtc
     6421 gatttgcgtt tgatggttcg gctggaattc gcataggtga tagtcatgaa gttattatct
     6481 cacgccacgt ggctgctcac attttggtca aatcctgaaa catggcacaa cccaataaac
     6541 aggtaactca actcaacatc aaaaaccgaa aggcttcttt cgagtatcag ttactcatgc
     6601 agtttagtgc cggtatcatg cttacgggta cagaaataaa atcggtacgt gccggaaaag
     6661 tcaacattgc cgatgcatac tgcacgtttg ttaaggatga attgtacgtg cgaaacatgc
     6721 acattcagga atacgaaatg ggaaatattt tcaatcacga acctaaacgt gatcggaaac
     6781 tacttttgaa taaaaccgaa ctgtcaaaac tgcgtggaaa aatgaaggat aaaggactaa
     6841 ccatcgtccc acttcaaatg tttctaagtg aaagtggatt cgctaaactc gacatcgccc
     6901 tagcaaaagg gaaaaaattg tacgataaac gcgaatcgct taaagacaag gatgtaaaac
     6961 gctcaatggc ccgacaagac gccgattaac ttttactttc caataaaggc acaaatctaa
     7021 attccccgtg ctcggcatct tgcaatgttc catcaagctg cttatcaata acatgcatca
     7081 cttgcttgcc atcaacgcct acgggaataa ctaatcttcc gccaggtttt aattgttcgg
     7141 tcaatgcctg tggaataaaa ggagctcctg ctgtgactaa tattttatca aatggagcaa
     7201 atgcgggttg acctaaatat ccatccccaa aaaacaaacg agccgatata ttcatcaact
     7261 ccagatgctt tttcgttcga atgtacaaag cgtgctgacg ttcgatacta aaaatcttca
     7321 ctccaaactg agctaataca gccgtttgat aaccactacc cgttcctatt tcgagcactt
     7381 tctcacctga ctttaattcc agtaattgtg tttgaaatgc tacggttgat ggatgcgaaa
     7441 tcgtttgtcc ggcaccaatc tgaaatgcaa tatcttcata tgccttctcc atgaaagcca
     7501 aatcttgaaa aaactgatgc ctaggaactg cacgtatcgc atccaaaaca ggttcactca
     7561 ttatgccttt ggctcgtagt tcgtctacaa gtcggtttct taatccttta agtcggtatg
     7621 aatcaggtat cagcatcgtt cttcagctcc ggagcacgaa aatactggtg cttgaagcat
     7681 tattgtgcag atggtctgtt cattcatcca caaacaggtg ttcatttttt tccgcttttt
     7741 aggtgttctt tcaatgcttc tcatcccatt ctttcggtga aaatgcgcta cgtttgcgca
     7801 aaattcagag agtatgctta agataggcgt agtcggtgct ggtcaccttg gaaagattca
     7861 cctaaaactc attggtgaaa tcgattcgta tcacctagcc ggtttttttg atgccgaccc
     7921 cgaagttgct gccagtgtag caaaagacat gaacatacat gccttttcaa ccttcgaaga
     7981 actattagat gcggtagatg tagtggatgt agttactcca acaatctacc atcatcaata
     8041 tgccagcaaa gcactccgat cgagcaaaca tgtttttatc gaaaagccga tcgccacaac
     8101 ggttgaagaa gcacgtgatt tagaacagtt ggctgttgaa gcaggtaaaa aagtacagat
     8161 tggacatgtt gaacgcttca atccggctta cgttgcagca atgccatttt gccaaaatcc
     8221 tatgttcatt gaaacacacc gtttggcatt attcaacccg cgagggacag atgtttcagt
     8281 tgtgcttgat ttaatgattc acgatatcga cattgtgctt catagcgtga attctacggt
     8341 taagaaaata agtgcttctg gagtttcagt tgttagtgaa acgcccgaca tcgctaatgc
     8401 acgtatcgaa tttgacaatg gctgtgtagc gaatttaact gctagtcgct tgtcgatgaa
     8461 aaacatgcgc aaaacacgat tcttccagcg cgatgcctac ataagtgtcg attttctgga
     8521 taaagaagtg gaagttattc gcatgagtac ggttgaaggc gaacctgatc ctttggcatt
     8581 aaccatagaa cttggtgagg gcaagggatt gaaacaaatc ttcctcgaaa aacccgaaat
     8641 tttaccaaca aatgcaataa aagctgaatt ggaaagtttt gctgatgcga tttcccacaa
     8701 tcagattccg gccgtaactg ccacgcacgg actccaggct ttggatgttg cttaccgcat
     8761 aattgaaaag atgaatacaa ctccgcatct gtttgcctaa gcatgaaata cggctacctg
     8821 atttaccttt ttatttgttt gagtctgttt gcaggatgcg atattatcaa tcctgaagaa
     8881 caagaagcag cctatatttc tatccaacag gtaaatctct caaccgatct tgatatacaa
     8941 ggaagtaatt cctccaacat aaaagatgct tggatttata ttgatgataa cctcattgga
     9001 gcctttcctt taccatgtaa aattccttct ttaaaaacag gaaatcacaa aatttcaatt
     9061 ggtgctggaa ttcaagtcaa tggttcttcc atgctaagaa caccctatat tttctatcgt
     9121 ttttatcaac aagaaaatgt aaatctggag gcaggatatg tgaccgaatt aaatcctgtt
     9181 gttacatatt ttgactcgat aacttttgga ttcaaggcta atttcgatga tttatctgga
     9241 aataagctca catcaacagc tgtaagcgac acaaccgttg aattaactac cgatgcaagt
     9301 aaagtgtttg aaggaacagg gtcatgtcta gcacgtttgt tacgcgatag cggattggtt
     9361 gaatttcaaa tgattgatcc agtaactcta cctaaaggag gacggaatat ttttctggaa
     9421 ctggattata aaacaactca cgatttagtc atcggacttc gctcttacta cacaggtgca
     9481 agtacaaagg aagaaattct gctttctttg cgggcaaatt ccaattggaa caaagtgtat
     9541 atcaatttaa ccaaagctgt aagtacccaa gtaaatgcag ctaattatcg aatctatttt
     9601 tcggcataca aacaagctgg atctgttccg cttgagattt tgctcgataa tcttaaaatt
     9661 gtttattaac gggtgatttc aactaaacgc gataaaattc aaggacgtat ccaagtcgcc
     9721 gccgactatt tttcggctgt gctcgggtgg accttatact acgcgtttcg gaagacctat
     9781 atcgagccta tcgtattcgg tcatcccgta cgaatggagt ttaacaatac cttttggctt
     9841 ggattagcca tcattccggt attctggctc attttacacc acatatcggg tgcttatttc
     9901 gatgtttacc gcaaatcgcg tctaggaatt ctaggaacaa ccgtttgggt tagcttactc
     9961 ggttcattga tattattctt cgctgtttca cttgacgatt ttatcctttc ctatcgttct
    10021 tactacatct ccttctctgc cctattcggg attcaatttg gtgttactgc cattcaacgt
    10081 atgttgattg ctacccgaag tgctcgaaga atccaatcgg gtaaagtgta tttcaatacc
    10141 ttattaatag gttcagggaa aaaagcactc gatatctata aaaagattga agaacaacct
    10201 gttagcagcg gtaatcgatt tttaggttac gttcctgtaa atggtacact cgatcccgaa
    10261 ctcagtaata cattgcaacg aatcggtcat ttcgatcaaa tggttcaatt gatccaagat
    10321 ttggatgtgg aagaagttat cgttgcaccc gaaaattcgg aacacgcata tctcggaaaa
    10381 ttcctaaccg aattagaagt aacaacggtt gtaattaaag taattcctga tatcaccgat
    10441 attcttttag ggtcagttcg gatgtcggca atttttggtg ctccagtaat cgaaatcaaa
    10501 cgtcaactca tgcctcgctg gcagaagtcg ttcaaacgat tgtttgatat caccgtttca
    10561 ctctttgttt tagtttgtct attccctgtt tatctcatca ctgcaataat tgtaaagact
    10621 acttcaagcg gtccagcact ttacagtcat cctcgaattg gattcaacgg taaaccattt
    10681 cgtatctata agtttcgttc gatgtattgc gatgccgaaa aacgtggacc gcaactcagt
    10741 agtaaaaacg attctcgcat tacaccattt ggtaaattca tgcgtaaaac acgactcgac
    10801 gaaattccac agttctataa tgtattggtg ggaaccatgt cgattgttgg tccgcgcccc
    10861 gaacgtcaat tctttatcga ccaaattgtt gaagttgcac cacattaccg tttgctacat
    10921 aaagttcgcc ctggaattac cggttgggga caagttaaat ttggctatgc cgaaaatgta
    10981 gatgaaatgg ttgagcgctt gaaatacgat attctgtata tcgaaaacat gaccttggcg
    11041 ctcgatttaa agattctgat ttatacgttt cttattgtcc ttcaaggaag aggaaaatag
    11101 aataatccag tttgaagttt cgatgggagt ttgcgttatt tgcactcctt tctgaatgct
    11161 cgtgcactca ggttaaatcg aaatgagatg aaaaaaattg ctgttattgg tgctggaaca
    11221 atgggaaatg gtattgcaca tacgtttgcc caatttggat ttcccgttat tttggttgat
    11281 gtaagtgatg ccgccttgag taaaggaatt caagcaatca cccaaaatct cgatcgccag
    11341 gttcaaaaag gtatcttaac agaagctcaa aaaacagaga ctttaactcg catttcaact
    11401 caaacacaaa tcgacgaacg tttgacggat cgtgatttag ttgtagaagc agcatccgag
    11461 aatttccaaa tcaaatcatc cgttttcaaa caattggatc aatattgtca gcctagctgc
    11521 atac
//
LOCUS       DAOOQW010000055        11421 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_7071_length_11421_cov_4.696639, whole genome shotgun sequence.
ACCESSION   DAOOQW010000055 DAOOQW010000000
VERSION     DAOOQW010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 11421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11421
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7071_length_11421_cov_4.696639"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..988)
                     /gene="carA"
                     /locus_tag="PK281_04925"
     CDS             complement(<1..988)
                     /gene="carA"
                     /locus_tag="PK281_04925"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HOY48722.1"
                     /translation="MQTTIPKTAVLLLEDGMAFYGKAVGKPFTSTGEICFNTGMTGYQ
                     EIFTDPSYLGQIVVATHVHIGNYGVNDLETESNSIKIAGLVCKSFSNHFSRIPAQGSL
                     TEYFEQQTIPAIEGIDTRAVVRYIRDKGAMNAIISSETTNLEELSTLLKNVPSMEGLE
                     LSSQVSTLEPYSVGDETSTYRVALLDLGVKSNIIRCLTERDCYVRVFPAKTSFSEMMD
                     FTPDGFMLSNGPGDPAAMGYAIETVKEILQSQVPMFGICLGHQVFAKAMGISTYKMFN
                     GHRGINHPIKNIITGLCEVTSQNHGFAVNAEEVKNHPEIEITHVHLNDQTIAG"
     gene            complement(1069..1563)
                     /gene="rplQ"
                     /locus_tag="PK281_04930"
     CDS             complement(1069..1563)
                     /gene="rplQ"
                     /locus_tag="PK281_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008464331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HOY48723.1"
                     /translation="MRHGKKLNHLGRTAPHRKAMMANIASSLILHKRINTTLAKAKAL
                     RVYVEPLITKSKEDTTHSRRTVFAYLKDKHAVAELFRDVSVKIADRPGGYTRIIKLGN
                     RLGDNAEMAMIELVDYNENLQKTAKAKVEKKTRRSGGAKKKADAPSTPAAETASESEE
                     TAAE"
     gene            complement(1601..2593)
                     /locus_tag="PK281_04935"
     CDS             complement(1601..2593)
                     /locus_tag="PK281_04935"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HOY48724.1"
                     /translation="MAILTFQRPDKVIMISSTDQHGQFEFRPLEPGYGITVGNALRRI
                     LLSSLEGFAITSIKIEGVEHEFSTIKGVVEDVTEIILNLKQVRFKRQIDSTEHEKVVI
                     SLNGQSQLTAGDIGKFTSSFQVLNHDFVICNMDPSVNLKMEITIDKGRGYVPAEENRP
                     ATQQIGLIPIDSIYTPIRNVKYSVENYRVEQKTDYEKLIIEITSDGSIHPKDALKEAA
                     KILIQHFMLFSDEKITEDVEEKVTTEEFDEDILHMRQLLKTRLVDMDLSVRALNCLKA
                     ADVDTIADLVSYNKNDLLKFRNFGKKSLTELEELVQSKQLNFGMNLAKYKLDKD"
     gene            complement(2637..3242)
                     /gene="rpsD"
                     /locus_tag="PK281_04940"
     CDS             complement(2637..3242)
                     /gene="rpsD"
                     /locus_tag="PK281_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018279609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOY48725.1"
                     /translation="MARYIGPKSKIARKFREPIFGPDKVLTKKNYPPGMHGNSKKRQK
                     VSEYGVQLQEKQKAKYTYGILEKQFALIFDRASRAQGVTGEMLLKLIEARLDNVVYRL
                     GLSNTRSGARQLVSHRHITVNGTVVNIPSYTLKAGDKIAVREKSKSMEVIQNAVSGHT
                     NKYSWLEFNASSLSGTFLNYPERELIPENIKEQLIVELYSK"
     gene            complement(3268..3657)
                     /gene="rpsK"
                     /locus_tag="PK281_04945"
     CDS             complement(3268..3657)
                     /gene="rpsK"
                     /locus_tag="PK281_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014166262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HOY48726.1"
                     /translation="MAKTTKTTKKRVVKVEAVGHAHINATFNNIIVSLTNTSGQVISW
                     SSAGKMGFRGSKKNTPYAGQMAAGDCSKVAYDLGLRKVKVFVKGPGGGRESAIRTIHN
                     SGIEVSEIVDVTPVPHNGCRPPKRRRV"
     gene            complement(3697..4074)
                     /gene="rpsM"
                     /locus_tag="PK281_04950"
     CDS             complement(3697..4074)
                     /gene="rpsM"
                     /locus_tag="PK281_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOY48727.1"
                     /translation="MARISGIDLPKNKRGEIGLTYIFGIGRSTAQKILNEAGISFDKK
                     VSEWNDDELGKIRDIINNTFKVEGALRSEVQLNIKRLMDIGSYRGIRHRSGLPVRGQT
                     TKNNARTRKGKKKTVANKKKVTK"
     gene            complement(4094..4210)
                     /gene="ykgO"
                     /locus_tag="PK281_04955"
     CDS             complement(4094..4210)
                     /gene="ykgO"
                     /locus_tag="PK281_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014790280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HOY48728.1"
                     /translation="MKVKTSIKKRSVDCKIVRRKGRLYVINKKNPKFKQRQG"
     gene            complement(4218..4436)
                     /gene="infA"
                     /locus_tag="PK281_04960"
     CDS             complement(4218..4436)
                     /gene="infA"
                     /locus_tag="PK281_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017185218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOY48729.1"
                     /translation="MSKQSSIEQDGTIIESLSNAMFRVELENEHVIIAHISGKMRMHY
                     IKILPGDKVKVEMSPYDLSKGRITYRYK"
     gene            complement(4457..5248)
                     /gene="map"
                     /locus_tag="PK281_04965"
     CDS             complement(4457..5248)
                     /gene="map"
                     /locus_tag="PK281_04965"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOY48730.1"
                     /translation="MIYYKTAEEIERIRISSLLVARALAEVAKIIGPGVTSLQLDKIA
                     EEFIRDNGGVPAFKGYGGFPNSLCISPNDQVVHGIPDNRPYESGEIISVDCGVLKDGF
                     YGDSAYTFGIGEVPEASKQLMQVTKESLLRGIGVALPGKRLHDISYEIQTYCEKHGYS
                     VVRELVGHGIGKNLHESPEVPNFGKRGSGIKLQEGLVIAIEPMINMGTKNVVHGKDGW
                     TIRTADKKPSAHFEHTIAIQKGKADILSSFEYVEEALKERNELVV"
     gene            complement(5261..6613)
                     /gene="secY"
                     /locus_tag="PK281_04970"
     CDS             complement(5261..6613)
                     /gene="secY"
                     /locus_tag="PK281_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009415363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOY48731.1"
                     /translation="MKNLINTIKNIYKVEELRTKIGNTLLFLLIYRLGSFVVLPGVDP
                     TALNVLKQQTSEGILGLLNMFSGGAFSNASIFALGIMPYISASIVIQLLTIAVPYFQK
                     MQKEGESGRKKMNQFTRYLTVIITAFQAPGYLANLNSQLGVNAISDHTAFFWISSTII
                     LVAGTMFIMWLGERITDRGIGNGISLIIMVGIIANLPAAILAEFGSRVEQQGGGMVMF
                     LLELVILLFIVIMSIMLVQGVRRIPVQVAKRIQGNKQVGGARQYIPLRVNAAGVMPII
                     FAQAIMFIPITIVGYAGIESLQGVMATFSNVTGFWYNFSFAVMIILFTYFYTAITVNP
                     KNMAEEMKRSGGFVPGIQPGRQTAEFIDSIMSRITLPGAVFLAVVAILPALAMMIGVT
                     NKFAQFYGGTSLLILVGVVLDTLQQIESHLLMRQYDGLMKSGRIKGRANAASPVSMAS
                     "
     gene            complement(6613..7062)
                     /gene="rplO"
                     /locus_tag="PK281_04975"
     CDS             complement(6613..7062)
                     /gene="rplO"
                     /locus_tag="PK281_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013305174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HOY48732.1"
                     /translation="MNLSNLKPATGSVKSSKRIGRGQGSGRGGTSTRGHKGAKSRSGY
                     SSKVGFEGGQMPLQRRVPKFGFKNINRVEYRGINLDTLQKLAEEKKLDAITFETLQQN
                     GLASKNDRIKVLGRGELTAKLAITVHAYTASAKTAIESLGGSATIIA"
     gene            complement(7079..7258)
                     /gene="rpmD"
                     /locus_tag="PK281_04980"
     CDS             complement(7079..7258)
                     /gene="rpmD"
                     /locus_tag="PK281_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020081796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HOY48733.1"
                     /translation="MSKLKITQIKSGIDRPERQKLTLRALGIRKMHNPVEHEATPQIL
                     GMVNKIKHLVSVEEI"
     gene            complement(7271..7789)
                     /gene="rpsE"
                     /locus_tag="PK281_04985"
     CDS             complement(7271..7789)
                     /gene="rpsE"
                     /locus_tag="PK281_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014085007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOY48734.1"
                     /translation="MANANIKRVRSSEIELKDKLVGVNRVTKVTKGGRAFTFSAIVVV
                     GNEKGIVGHGLGKAKEVTDAISKGVDDAKKTLIKVPVINGTVPHEQIGKFGGALVFLK
                     PAAHGTGVIAGGAMRAVLESVGITDVLAKSKGSSNPHNVVKATIDALSQMRDPATIAQ
                     QRGITMEKVFNG"
     gene            complement(7797..8153)
                     /gene="rplR"
                     /locus_tag="PK281_04990"
     CDS             complement(7797..8153)
                     /gene="rplR"
                     /locus_tag="PK281_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013996572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOY48735.1"
                     /translation="MAITKEYRRARIKERIRKVVVGDANAPRMSVYRSNKDIYVQLID
                     DSKGHTLLAASSSEASISSKKVNKIEQAALVGKAIAAKALEAGISEVRFDRNGYLYHG
                     RVKSLAEGAREGGLKF"
     gene            complement(8169..8726)
                     /gene="rplF"
                     /locus_tag="PK281_04995"
     CDS             complement(8169..8726)
                     /gene="rplF"
                     /locus_tag="PK281_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOY48736.1"
                     /translation="MSRIGKMPVTLPKGVTVSVSSSNLITVKGPLGELHQAVDPAIQL
                     SVEEGNVTVTRQNDDKDQRAKHGLYRSLIANMVKGVSEGFTTEQELVGVGYRAANTGQ
                     LLELSLGYSHNITFELPKEIQVETKTERGKNPLIIMKSTDKQLLGMVAAKIRSLRKPE
                     PYKGKGIKFVGEELRRKAGKSAGKK"
     gene            complement(8748..9152)
                     /gene="rpsH"
                     /locus_tag="PK281_05000"
     CDS             complement(8748..9152)
                     /gene="rpsH"
                     /locus_tag="PK281_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOY48737.1"
                     /translation="MTDPIADYLTRLRNAINANHRVAEIPSSKMKREMTKILFEKGYI
                     LSYKFEDASATDPQGTIKIALKYHPVTKIPAIRTLSRVSKPGLRKYSHVANIPRVING
                     LGIAILSTSKGVMTDKEARRENVGGEVLCYVY"
     gene            complement(9186..9455)
                     /gene="rpsN"
                     /locus_tag="PK281_05005"
     CDS             complement(9186..9455)
                     /gene="rpsN"
                     /locus_tag="PK281_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HOY48738.1"
                     /translation="MAKESMKAREVKRAKMVERYAEKRAALKAAGDSVGLQKLPRNSS
                     KIRLRNRCKLTGRPRGYIRQFGLSRITFREMASDGKIPGITKASW"
     gene            complement(9463..10020)
                     /gene="rplE"
                     /locus_tag="PK281_05010"
     CDS             complement(9463..10020)
                     /gene="rplE"
                     /locus_tag="PK281_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019386279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOY48739.1"
                     /translation="MEYTPNLKTKYRKEISVALLNEFGYKSKMQVPKLEKICINQGIG
                     AAVSDKKLIDSAVAEMTMITGQKAVPTKSKKDISNFKLRKGVAIGARVTLRGDKMYEF
                     LERLIVVALPRIRDFRGINDKGFDGRGNYTLGVTEQIIFPEINIDKVTRIMGMDITFV
                     TNAETDKEAFALLREFGLPFKNLKK"
     gene            complement(10020..10337)
                     /gene="rplX"
                     /locus_tag="PK281_05015"
     CDS             complement(10020..10337)
                     /gene="rplX"
                     /locus_tag="PK281_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016196843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HOY48740.1"
                     /translation="MKLHIKKGDKVTVLSGDSKGQIGEVREIDTKKMRAIVIGVNMIS
                     KHAKASTKYPQGGIIKMEGPVHISNLMLIEPKTGKPTRTGRKADANGKMVRFSKKSGE
                     IIK"
     gene            complement(10353..10721)
                     /gene="rplN"
                     /locus_tag="PK281_05020"
     CDS             complement(10353..10721)
                     /gene="rplN"
                     /locus_tag="PK281_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HOY48741.1"
                     /translation="MIQQESRLTVADNSGAKEVLCIRVLGGTKKRYASVGDKIVVTVK
                     HALPSGNAKKGSVTKAVVVRTKKEIRRADGSYIRFDDNAVVLLNNNDELRGTRIFGPV
                     ARELREKQFMKIVSLAPEVL"
     gene            complement(10750..11013)
                     /gene="rpsQ"
                     /locus_tag="PK281_05025"
     CDS             complement(10750..11013)
                     /gene="rpsQ"
                     /locus_tag="PK281_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016660790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HOY48742.1"
                     /translation="MEAIERNLRKERTGLVRSNKMDKTIVVAVERKVKHPKYGKFVKK
                     TTTFFAHDDKNECGIGDTVKISETRPMSKNKRWRMVEIVERAK"
     gene            complement(11021..11224)
                     /gene="rpmC"
                     /locus_tag="PK281_05030"
     CDS             complement(11021..11224)
                     /gene="rpmC"
                     /locus_tag="PK281_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HOY48743.1"
                     /translation="MKQVQVQELSLVELKDRITEETQVLAKMVFNHTVSTIENPLKIR
                     TTRRAIARMKTELRKRELASTAN"
     gene            complement(11231..>11421)
                     /locus_tag="PK281_05035"
     CDS             complement(11231..>11421)
                     /locus_tag="PK281_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009087332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribosomal protein L16"
                     /protein_id="HOY48744.1"
                     /translation="VRMGKGKGAPEYWVAVVKPGTIIFEAEGVPMEIAKEALRLAAQK
                     LPVTTKFIVRRDYVEATA"
BASE COUNT         3078 a         2448 c         2121 g         3774 t
ORIGIN      
        1 tacctgctat ggtttgatca tttaaatgga catgtgtaat ttctatttca ggatgatttt
       61 ttacttcttc agcattaact gcaaaaccat gattttgtga tgttacctca cataatccag
      121 tgataatatt ttttattgga tgattgattc cacgatgtcc attaaacatc ttataggtgc
      181 ttattcccat cgctttagca aaaacctgat gtcctaagca tataccaaac ataggtactt
      241 ggctttggag tatttctttt actgtttcga ttgcgtaacc catagctgct ggatctccag
      301 gcccgttcga taacataaag ccatcagggg taaaatccat catttctgaa aaactagtct
      361 tagccgggaa tactcgaaca taacaatctc tttccgttag acagcggatg atattacttt
      421 taacacccag atcaagcaat gcaacgcgat atgttgaagt ttcatcacca actgaatatg
      481 gctcaagagt agacacttga gatgaaagct ccaaaccttc catagaaggg acatttttca
      541 atagagtact aagctcttct aagttagtgg tctcactcga tatgattgca ttcattgcac
      601 ccttatctcg aatataccga actactgcac gtgtatcaat tccttcaatg gctggaattg
      661 tctgttgttc aaaatattcc gttaaagaac cttgagcagg aatccgagaa aaatgattag
      721 aaaagctttt acaaactaat ccagcaatct taattgaatt tgattctgtt tctaaatcat
      781 taacaccata attcccaata tgaacatgtg tagcaactac aatttgtcct aaataggatg
      841 gatcagtaaa tatttcttga tacccagtca taccggtatt aaaacaaatt tcaccggttg
      901 aagtgaatgg ttttcctaca gccttgccgt aaaatgccat tccatcttcc aataatagta
      961 ctgctgtttt ggggattgtt gtttgcataa ttttcgtggg caagatcaaa aaaaaaggat
     1021 aaaaccactt ggctttatcc tttcaaaatg ttcacaatgt tgatgacatc actcagctgc
     1081 tgtttcttcc gattcagatg ctgtttcagc tgccggtgta gaaggagcat cagctttttt
     1141 cttagcccca ccactacgac gagttttctt ctcaactttt gctttcgcag ttttttgaag
     1201 attttcgttg tagtcaacca actcaatcat cgccatttca gcattatcgc ctaaacggtt
     1261 accaagttta atgatacgtg tgtatccacc tggacggtca gcaattttca ctgaaacgtc
     1321 gcgaaataac tcagcaactg catgcttgtc tttaagataa gcaaatacgg tacgacggct
     1381 atgtgtagta tcttcttttg atttggtaat caaaggttca acatatacgc gcagcgcttt
     1441 ggctttagca agtgtagtat tgattcgttt gtgtagaatc aatgaacttg caatattcgc
     1501 catcatcgct ttccggtgcg gtgcggtgcg accaaggtga tttaattttt taccgtgtct
     1561 catgatgcaa tgtaatgttc tttagcctct cgggctgtga ttagtctttg tcgagtttgt
     1621 atttggccaa attcattccg aagttcaatt gcttgctttg aactagttct tcaagttcag
     1681 tcaacgactt cttaccaaag ttgcggaatt tcaataggtc atttttgttg tatgatacaa
     1741 gatcagcgat agtatctaca tcagctgctt taaggcagtt taatgcacgt acacttaaat
     1801 ccatatcaac taaacgagtt ttcaaaagtt gacgcatgtg aaggatatct tcatcaaatt
     1861 cttcagtcgt tactttttct tctacgtctt ctgtaatctt ctcatcagag aataacatga
     1921 agtgctgtat caaaatctta gctgcttctt tcaaagcatc tttcggatgg atagatccat
     1981 cacttgtgat ttcgataatc agtttctcat aatccgtttt ttgctcaaca cggtagtttt
     2041 ctactgagta ttttacatta cggattggag tgtaaataga gtcaattgga attaatccaa
     2101 tttgttgtgt agctggacga ttttcttccg ctggaacata tccacgacct ttgtcaatgg
     2161 ttatttccat tttcaagttc acagatggat ccatgttaca gatcacaaaa tcgtgattta
     2221 aaacttgaaa tgatgaagtg aatttaccaa tgtcgcccgc tgttaattgg ctttgaccat
     2281 taaggctgat cacaactttt tcgtgctcag tactatcgat ctgacgttta aagcgaactt
     2341 gtttaaggtt tagaatgatc tcagtaacat cttcaaccac gcctttaatg gtggagaatt
     2401 catgttccac tccttcaatt tttattgaag taatggcaaa tccttctaat gaagaaagaa
     2461 gaatccgacg gagagcatta ccaactgtaa ttccataccc tggttcaagt ggtctgaact
     2521 caaactgacc atgttgatca gttgaagaga tcatgattac tttgtcggga cgttgaaaag
     2581 tcagtatagc catagtattc gtggtttgaa tggtgaggta agagaagtgt aacgaattat
     2641 ttcgagtaca attccacaat cagttgttcc ttaatgttct caggaatcag ttcacgttct
     2701 ggataattca agaatgttcc gctgagtgat gatgcattaa attccaacca tgaatatttg
     2761 ttggtatgtc cagaaacagc attctgaata acttccattg atttagactt ctcacgcaca
     2821 gcgattttat cacctgcttt caaagtataa gatggtatgt taactactgt tccgtttact
     2881 gtaatgtgac gatgagaaac taattgacga gcgcctgaac gtgtattaga aagacctaaa
     2941 cggtaaacta cattgtctaa acgagcttca attaatttaa gcaacatctc acctgttact
     3001 ccttgtgcac gacttgcgcg gtcaaaaatt aacgcaaatt gtttctcaag gattccgtaa
     3061 gtatattttg ctttttgctt ctcctgaagc tgaactccat attcagaaac tttttgacgc
     3121 ttcttagagt tcccatgcat tccgggagga tagtttttct tcgtgagcac cttgtccgga
     3181 ccaaagattg gttcacggaa cttacgggcg atcttggatt tcgggccgat atatcttgcc
     3241 atgaggttgt attgttaaaa tgaatgatta aacgcggcgg cgttttggtg gacggcaacc
     3301 attatgtggt accggtgtta catcaacgat ttcgcttact tcgataccgc tattgtggat
     3361 tgtacgaatt gcagattcgc gaccaccgcc tggtccttta acaaacacct tcactttgcg
     3421 aagacctagg tcgtaagcaa cttttgaaca gtcgcctgca gccatttgac ccgcataggg
     3481 tgtgtttttc ttagatccgc gaaatcccat cttgccagct gaactccagg aaattacctg
     3541 gccacttgtg ttggtgaggg atacaataat attgttaaag gttgcattaa tgtgagcgtg
     3601 acccactgct tccactttaa caacacgctt cttggttgtt ttggttgttt tagccatttc
     3661 ttaatggatt ctacgattgt ttcggattca ggaatattat ttagttacct tcttcttgtt
     3721 ggcaactgtt ttcttcttcc ctttccgggt acgtgcatta tttttagtgg tttgtccacg
     3781 aactggtaaa ccagaacgat gacgaatacc gcgataggat ccgatatcca ttaaacgctt
     3841 aatgttcaac tgaacttctg aacgaagtgc accttctact ttaaacgtat tgttaatgat
     3901 atcacggatc tttcctaatt catcatcatt ccactccgat acttttttat cgaaggaaat
     3961 tcctgcttca ttgaggattt tctgtgctgt agagcggccg atcccaaata tataggtcag
     4021 tccaatttct ccacgcttat ttttaggaag gtcaattccg gaaatacgag ccatgttgta
     4081 tgctgtttgg tgcttatcct tgacgttgtt tgaacttagg attctttttg ttgattacat
     4141 aaagtctccc tttgcggcgt acaatcttgc agtccacact ccgtttctta atggaggttt
     4201 ttactttcat tgtgttatta tttgtagcga taagttatcc gaccttttga caaatcgtat
     4261 ggagacatct ccacttttac cttatcaccg ggtaaaatct tgatatagtg catacgcatc
     4321 tttccagata tgtgtgcaat gataacgtgc tcgttctcca gttccacacg aaacattgca
     4381 ttcgacaatg attcgataat ggttccgtct tgctcgatgg aggactgttt tgacacgttt
     4441 attattcgtt tttatgttaa actaccaatt catttcgttc tttcagagct tcttcaacat
     4501 attcaaatga agaaagaata tctgccttgc ctttttgaat agcaatcgta tgttcaaaat
     4561 gtgctgaagg tttcttatcc gcggttcgta tggtccaacc atcttttccg tgaactacat
     4621 tcttcgttcc catattaatc ataggttcaa ttgcaatcac taacccttcc tgtagcttaa
     4681 tccctgaacc acgttttcca aagtttggca cttcgggtga ttcatgtaga tttttaccaa
     4741 ttccatgacc aactaattca cgaacaactg aatatccatg tttttcgcaa taggtctgaa
     4801 tttcgtagct gatatcatgc aaacgttttc caggtagtgc aaccccgatt cctcgaagta
     4861 aactctcttt ggtcacttgc attaattgct tggatgcttc aggaacctca ccaataccga
     4921 atgtataggc agaatcccca taaaatccat cttttaaaac tccacaatca acagaaataa
     4981 tttctcctga ttcataagga cgattatctg gtatcccatg tacaacctga tcattaggtg
     5041 aaatacataa ggaatttgga aaccctccat aacctttaaa agcaggaact cctccattat
     5101 cacgaatgaa ttcctcagca attttatcca gctgcaaact ggttacccca gggccgatga
     5161 ttttggcaac ttccgctagg gcgcgggcaa caagtaaaga actaattctt atgcgttcaa
     5221 tttcttcggc ggtcttgtaa tagatcatcc aaaaaaattg ttagctagcc atacttaccg
     5281 gagaagctgc gtttgcacgc cccttgattc ttccactctt catcaaacca tcgtattgac
     5341 gcatcaataa atgactttca atttgttgaa gtgtatctaa aacaacccct actaaaatta
     5401 gaagagatgt tcctccgtaa aattgtgcga atttatttgt cacaccgatc atcatggcca
     5461 aagcaggtag aattgctact actgctaaga atactgcacc cggaagtgta atacgtgaca
     5521 taatactgtc gatgaattca gctgtttgac gaccaggttg aattcccggt acaaatcctc
     5581 cactacgttt catttcttca gccatatttt tcggattaac cgtaatagct gtatagaaat
     5641 aagtgaagag tataatcatt actgcaaatg agaagttata ccagaaacct gtcacgttag
     5701 agaatgttgc cattacccct tgtaaagatt caattccagc ataccctaca attgtaatag
     5761 ggataaacat aattgcttga gcaaaaatga ttggcattac acccgctgca tttacacgta
     5821 acggaatata ctgacgagct ccaccaactt gtttatttcc ttggatacgt ttagctactt
     5881 gtactggtat tcttctaact ccttgaacaa gcataatcga catgattacg atgaacaata
     5941 gaataacaag ttctagtaag aacattacca taccaccacc ttgttgctca acacgtgaac
     6001 caaactcagc taaaatagca gctggaagat tggcgatgat accaaccatg ataattaatg
     6061 aaataccatt accaattcca cgatcagtaa ttcgctctcc taaccacata ataaacatgg
     6121 taccagcaac tagaataata gtagaagata tccagaaaaa tgcggtatga tcagaaatgg
     6181 catttactcc aagctggcta ttaagattcg ctaaataacc cggtgcctga aacgcagtaa
     6241 taataacggt taggtaacga gtaaattgat tcatcttttt tctgccgctc tccccttctt
     6301 tctgcatttt ttggaaatat ggaacagcaa tagtcaaaag ctgaataaca attgaagcag
     6361 agatgtatgg catgataccc aatgcaaaga tggaagcatt ggaaaatgcc ccacctgaaa
     6421 acatattgag taatcctaga atcccttctg aagtttgttg cttcaatacg tttaatgcag
     6481 ttggatcaac accaggcaat accacgaacg agcccagtct ataaatgagc aggaaaagaa
     6541 gcgtattgcc aattttggta cgaagctcct ctactttgta tatgttcttg atggtattga
     6601 tcagattctt catcaggcaa tgattgtagc gctaccgcca agtgattcaa tagctgtttt
     6661 tgcagaagct gtataagcgt gaactgtgat agctaattta gctgtcaatt ctccacgtcc
     6721 taatacttta attcggtcat ttttagaagc taaaccgttt tgttgaagcg tttcaaatgt
     6781 gatagcatcc agtttcttct cttcagcaag tttctgaagt gtatcgaggt tgataccacg
     6841 gtattcaaca cgattgatgt tcttaaatcc aaatttagga actcgacgtt gaagtggcat
     6901 ttgacctcct tcaaaaccta ccttacttga atatcctgaa cgtgacttag ctcctttatg
     6961 tccacgggta gatgtaccac cacgaccgga accttgtcca cgaccaatcc gcttgctgct
     7021 ttttactgag ccagttgccg gcttaagatt actgaggttc atttctcaat gatttttttc
     7081 agatttcttc tacagaaacc agatgtttaa ttttatttac cattccaaga atctgtggag
     7141 tggcttcatg ctcaactgga ttgtgcattt tgcgaatccc aagagcacgt agagtcaatt
     7201 tctgacgctc cggacggtca ataccgcttt ttatctgagt gatttttagt tttgacatgg
     7261 gaggcaacta ttatccgttg aatacttttt ccattgtaat accacgctgt tgagcaatgg
     7321 ttgctggatc acgcatttgg cttaaagcat caattgttgc tttaaccacg ttgtgtggat
     7381 tagatgatcc ttttgatttt gcaagaacgt cggtaatacc tacactctct aatacagcac
     7441 gcatcgcacc accagcaatt acaccagttc catgtgcagc aggtttcaag aaaaccaatg
     7501 ctccaccaaa ttttccaatt tgctcatgtg gtacagttcc gttaattact ggaactttaa
     7561 tcaaagtctt cttagcatca tcaacacctt tgctaatcgc atcagttacc tcttttgctt
     7621 ttccaagtcc gtgtcctaca attccttttt catttccaac aacaacaatt gctgagaatg
     7681 taaaggcgcg accacctttg gttactttgg ttacacgatt tacgccaacc agcttgtctt
     7741 ttaattcaat ttcgctggag cgaactcgtt tgatatttgc gtttgccatg ttctgattag
     7801 aattttagac caccttcacg tgcaccttcg gctagagatt tcacccgacc gtggtaaaga
     7861 taaccatttc ggtcgaagcg aacttcactg attccagctt ctaaggcctt cgcagcaata
     7921 gctttcccta ctaaggcagc ttgttcgatc ttgttaactt ttttagaaga aatcgatgct
     7981 tcagaagaag aagcagccaa tagggtgtga cccttactgt cgtcgatcaa ttgaacgtag
     8041 atgtccttgt tgctgcggta aacactcatg cgaggtgcat tagcatcgcc aacaaccact
     8101 ttgcggatgc gctctttgat tcgcgctctt ctgtattctt tcgtgatagc catgatccgt
     8161 aatgattctt atttcttacc agcagattta ccagctttgc ggcgaagttc ttctcctacg
     8221 aacttaatac ctttaccttt atatggttca ggtttacgta atgatcgaat cttagctgcc
     8281 accataccca gtaattgttt gtcggtggat ttcataatga taagcgggtt cttaccacgc
     8341 tcagttttgg tttcaacttg aatttctttc ggtaactcaa acgtaatgtt atgagaatat
     8401 ccaagagaaa gttctagaag ttgtccagta ttagcagcac ggtaacccac acctactaat
     8461 tcttgttctg ttgtaaaacc ttcagataca ccttttacca tgttggcaat aagtgaacga
     8521 tatagaccat gcttagcgcg ttgatcttta tcgtcgttct gacgagttac tgttacgttt
     8581 ccttcttcaa cactaagttg aattgcagga tctacagctt gatgtaattc gcctagtggc
     8641 ccttttacag taatcagatt gctagacgat acagaaactg taactccttt tggcaacgtg
     8701 acaggcattt ttccaatacg tgacatgatt cttttcctct ttggcgatta ataaacgtag
     8761 cagagaactt ctccacctac attttcacga cgtgcttcct tatcggtcat cacacctttt
     8821 gatgtagata gaatggcaat acctaatccg ttgattacgc gtggaatatt tgccacatgt
     8881 gaatatttac gtaatcctgg ttttgataca cgagatagtg tacgaattgc agggattttc
     8941 gtaaccggat gatatttcaa tgcgattttg atcgtgccct gtggatcagt ggctgatgcg
     9001 tcttcgaatt tgtaactgag gatgtaccct ttttcaaaaa ggattttagt catctcacgt
     9061 ttcatcttcg acgatggaat ttcagctacg cgatggttag cgttgatggc attccggagc
     9121 ctggttagat aatctgcgat cgggtcggtc attttgaatt gtattttatt gattctttaa
     9181 gcgaattacc agctggcttt tgtaatccct gggattttcc catcagatgc catttcacgg
     9241 aatgtgatac gtgacaatcc gaattgacgg atatatccac gaggacgacc cgttaatttg
     9301 caacgattac gaagacgaat cttcgaagag ttgcgtggaa gcttttgaag tccaactgaa
     9361 tctcctgcag cttttaatgc tgctcttttt tcagcataac gttcaaccat tttcgcccgt
     9421 tttacctcgc gggctttcat gctttcctta gccattggtc gcttattttt ttaaattttt
     9481 gaaaggaaga ccaaactcac gtaatagtgc gaatgcctct ttatctgttt ccgcattggt
     9541 tacaaacgta atgtccatac ccatgatacg tgttactttg tcgatgttga tttctgggaa
     9601 gataatctgc tcagttacac ccaaagtgta atttccacga ccatcaaatc ctttatcatt
     9661 aattccgcgg aagtcacgaa tacgtggtaa agctaccaca ataagtcttt ccaagaattc
     9721 atacatcttg tcgccacgta aagtaacgcg agctccaatt gcaactcctt tacgaagttt
     9781 gaagttggaa atatcttttt tcgatttcgt aggaactgct ttttgaccag taatcatagt
     9841 catttcagct acagctgaat caatcagttt cttatccgaa acagccgcac caataccttg
     9901 attgatacag atcttttcca atttaggtac ttgcattttg cttttgtacc cgaattcgtt
     9961 cagtaaagca acagaaatct ctttgcgata cttggttttg aggttaggtg tgtattccat
    10021 cacttaataa tctcccctga ttttttagaa aaacgtacca ttttaccatt agcatctgct
    10081 ttacggccag tgcgggtagg ttttccggtt tttggttcga tcaacattag gttggaaatg
    10141 tgaacaggtc cttccatttt gatgatcccg ccttgcggat atttcgtgct cgctttagca
    10201 tgtttggata tcatgttaac gcctattaca atagcacgca tttttttagt atcaatttca
    10261 cgtacttctc caatctgacc tttagagtca cccgatagta ctgttacttt atctcctttt
    10321 ttgatgtgaa gtttcatcgc tctgtagctg atttaaagta cctctggtgc aagggaaacg
    10381 attttcataa actgtttttc acgcagttca cgtgctaccg gtccgaaaat acgggtacct
    10441 cgtaattcgt cattattatt taacagcacc acggcattgt catcaaatcg gatgtacgag
    10501 ccatcagcgc ggcgtatttc ttttttcgtg cgtaccacaa ccgctttggt tacggagcct
    10561 ttctttgcat ttcctgatgg taatgcgtgt tttactgtaa ctactatctt gtcgcctacc
    10621 gaagcgtagc gtttctttgt tccgcctaat acgcggatac ataacacctc tttggcgcca
    10681 ctattatccg ctactgtaag acgagattcc tgttgtatca tgacttagtt atgattctgt
    10741 tcggttcaat tatttcgcac gctctacaat ttccaccatt ctccaacgct tgttcttgct
    10801 cattggacgg gtttcactga ttttaacggt atctccgatg ccgcactcat tcttgtcatc
    10861 gtgagcaaag aatgttgttg ttttcttcac aaacttaccg tacttcggat gttttacttt
    10921 acgctcaaca gctacaacaa tggttttgtc cattttgtta ctgcgaacca atccggtacg
    10981 ctcttttcga aggtttcttt caatagcttc catcagcttc ttaattagcg gttgatgcga
    11041 gttcacgctt gcgaagttct gtcttcatac gggctattgc acgacgtgta gtacggatct
    11101 tcagtggatt ttcaatagta gaaactgtat ggttaaatac catcttagct aatacttgtg
    11161 tttcttctgt gattctgtct ttcaactcaa cgagtgaaag ctcctgaact tgaacttgtt
    11221 tcataactta tcaggcagtt gcttctacgt aatcacggcg tacgatgaat ttcgtcgtta
    11281 ccggtagttt ttgtgctgct aatcgaagag cttctttggc aatttccatt ggaactcctt
    11341 ctgcttcgaa aatgatggtt cctggcttaa caacagctac ccaatattca ggtgctcctt
    11401 tacctttacc catacgaacc t
//
LOCUS       DAOOQW010000056        11337 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_7162_length_11337_cov_5.718490, whole genome shotgun sequence.
ACCESSION   DAOOQW010000056 DAOOQW010000000
VERSION     DAOOQW010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 11337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11337
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7162_length_11337_cov_5.718490"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..708
                     /locus_tag="PK281_05040"
     CDS             <1..708
                     /locus_tag="PK281_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48745.1"
                     /translation="DADLLNRMASKQGLLYRSAIISNDYYALKQLYQEDSLIKGNTEL
                     VNWLLLKGIYDLQNIRDSKLEQSYGLLLKMKRDSSLSPKIQLEVNRILATLQPKIKGE
                     PFPDLGCHDQPYWKQQKWKGLPVYLAILPNYAPSSQLVIRQFAALQTKYGKEMHFALL
                     IPEADANEMNMLNKQFPELDLGQMDLCREKVQEIIPSTSRTNFILIDRLGNVFQNPAE
                     GPETDVESAFLGLIKLH"
     gene            complement(695..1330)
                     /locus_tag="PK281_05045"
     CDS             complement(695..1330)
                     /locus_tag="PK281_05045"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOY48746.1"
                     /translation="MSQKYAVYLNRKALFFNNPQGIQPQGDTVWIDAGASKKQLFEAV
                     ERLKSAKNECQQLSLTAINLDQGTEWLKSKYSFIQAAGGIVETPDNQILFIFRLGCWD
                     LPKGKVEPGEDLPSAAQREIEEETGILDLSIQSELCRTWHTYPHKGKEVLKETVWYWF
                     KTSKAWPTIPQTEEDITEAVWKSPDQWTEVEANTYPSILDVLQARKAISEA"
     gene            1329..1985
                     /gene="pyrE"
                     /locus_tag="PK281_05050"
     CDS             1329..1985
                     /gene="pyrE"
                     /locus_tag="PK281_05050"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HOY48747.1"
                     /translation="MLYNTDTAAQLADFLLQIKAIKLQPSKPFVWASGWKSPIYCDNR
                     LTLSYPKVRTFIRQELAKAIADKFEKPDVIAGVATGGIAHGALVAQELGLPFIYVRSA
                     PKGHGMENLVEGKIEPGQTVVVVEDLISTGQSSIKVVKALRDAGCIVKGLVSIFDYGF
                     DVAVAQFKEDKCPFYSLSNYDSLLDQAVKNKYVALEEIDQLKSWRDNPSSWGATLKIA
                     "
     gene            1989..2381
                     /locus_tag="PK281_05055"
     CDS             1989..2381
                     /locus_tag="PK281_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48748.1"
                     /translation="MKLETSWKPVQTTPDQVFAFLSDMNNLKKLMPEQVINWTSDTDT
                     CQFTIKGMADLGMKVVERNEHTSIKMSSHGKVPFPFTLTVGIRPLDAISEAQLVFDGE
                     VNPFLKMMVEKPLGNFFNMLLDGLGRQF"
     gene            2523..3002
                     /locus_tag="PK281_05060"
     CDS             2523..3002
                     /locus_tag="PK281_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48749.1"
                     /translation="MKNTLKLAAVALIAVFVVACSGGNGPETNAKAYLDALNAEDFDK
                     AKEFATEDTKGMLDFMKQIATMGGEKKEAKEKKEIKDLKCTVEGDTTATCTYMAGDKE
                     EKLNMKKSGEKWLVHQPKENPMGGGEEGGAMEGDMGTTDTSATAAPAAEAAPAAESH"
     gene            3141..3668
                     /locus_tag="PK281_05065"
     CDS             3141..3668
                     /locus_tag="PK281_05065"
                     /inference="COORDINATES: protein motif:HMM:NF017519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX/YebB-like N1pC/P60 family cysteine
                     hydrolase"
                     /protein_id="HOY48750.1"
                     /translation="MSQQEKGLLHSGDIILRRGYGMFSDGIVEVQNSPAAVSHCAMIV
                     GEPGKWRVAHALSSSVAAIDGAQEQSLQRFLNESVPNSIIVKRFRCQEDTIARIVDRV
                     SYYTRIHKPFDHNFDKVDTTRFYCTELFQHCFKDVLGRDIFLGQLDSNTTGIYDLSTF
                     QDTSLFETIIDHHTH"
     gene            3745..4764
                     /locus_tag="PK281_05070"
     CDS             3745..4764
                     /locus_tag="PK281_05070"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOY48751.1"
                     /translation="MKKIITSSLLLLGLISAKAQNEAAIAAYNQGISEYNVKDFQTAL
                     GSFNKAIESDSTFGKAWFNRAVCKSDLKDYAGAITDYQRSMKIEGENAKTLFFIGQAR
                     FLSADKTGALEDFKKSSQLDPTNAESFFYAGGILFEIDDFQGATEEFTKAIKARSDYA
                     EAYNDRGSCKLKLEDIKGAIDDYREATKLNPKLAIAYNNLAAALRRSGDLQASLQEFN
                     YSIIADPKMATSYVKRGRLKLEMQDLDGAMTDFNKAIELNPKDQYALNNRGCLKNKLR
                     DFPGAIADFDQAIRINPSYGYAYLNRGIAKENLRDLAGACKDWSEASQNGVDAAKQYV
                     DSECN"
     gene            4773..6836
                     /locus_tag="PK281_05075"
     CDS             4773..6836
                     /locus_tag="PK281_05075"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOY48752.1"
                     /translation="MKKILLLALFMLPVFIQAQQNVEFTKANFPDNKAGLKEAIKNIE
                     TGDSYYLAADYLKYLAVDFYVKANNFNPNNALLNFRLGHSYLNSPNKYKALDFLKKAY
                     QMNPSIDPEVTFYLGEAYHLNHDFDNAIKYYSMYKSSATKKPGFDPDKRITECNEGKR
                     LVANPVRVMIENLGPVVNSKYREYAAVITADESVMLFTSRRDNTTGQAIAEDGMFYED
                     ILSTTKQNKAWTPPVNLPAPVNTKLHDATIALSADGQKLFIYTDENEGDVLLSKLEGD
                     KWSKPEQAGGNAINTKYSEIHACFSYDEKSIYFVSNKPEDNYGGFDIYVTHQDANGRW
                     GKPENLGPAINTIYDEEGVYLHPDGKTMYFSSKGHKTMGSYDIFRSVFDDGKWSEPEN
                     IGYPINSADKDAFFVVSASGKHGYYSSARMDAIGETDIYMITLLGPEKEPILNVEDQL
                     LLSRMMITGEKARAESVVASKTDKNKFRSEVTILKGTIVDALDKHPLNAIIVITDNEK
                     NAIVSTFQANSKTGQYLVSLPSGRNYGIAVTMEDYLFHSENIDLPKSTAYQEIVKDVE
                     LSKLEIGKKIVLRNIFYDFDKATLRPGSMAELDRLAKLLEQNPKIRIEISSHTDNVGS
                     AAYNQKLSTNRALSVVDYLVDKKGISKNRLEFKGYGFDDPIAPNDTDQGRQLNRRTEF
                     KILSK"
     gene            6873..7535
                     /gene="mnmD"
                     /locus_tag="PK281_05080"
     CDS             6873..7535
                     /gene="mnmD"
                     /locus_tag="PK281_05080"
                     /inference="COORDINATES: protein motif:HMM:NF033855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD"
                     /protein_id="HOY48753.1"
                     /translation="MIQLLPTSDGSTTLYDSNLDETYHSVHGALQESMHVFIEKGLRL
                     RLPEQKHIRILEIGFGTGLNALLSLRETKLDPTLTVDYWCLEPFPISKSIYLQLNYTQ
                     PEEQANFLAFHQAEAGRSISINSSFTFRRLEQSIDGFTSEQMFDMVFYDAFGPPSQPE
                     LWTTETLKYSVDVLSPGGIWVSYCAKGDVRRSLISLGLQAKRLPGPPGKRHMLFAQKG
                     NV"
     gene            7566..8027
                     /locus_tag="PK281_05085"
     CDS             7566..8027
                     /locus_tag="PK281_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007094292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4920 domain-containing protein"
                     /protein_id="HOY48754.1"
                     /translation="MKKTLTFLMLLVFGAIQVNAQKAEYFGEKIKKDGAISTAEFLKM
                     MEGKDSLVVKIEAPINEACQKKGCWMNVDLGGGKEMMVRFKDYGFFVPKDCNGKKAIM
                     NGIAYREVVSVEMLRHYAEDAGKSKEEIMKITQPETKLSFEADGVIIYAVK"
     gene            8030..8425
                     /locus_tag="PK281_05090"
     CDS             8030..8425
                     /locus_tag="PK281_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48755.1"
                     /translation="MYRIAFLIAIVSMCFISASAVKQTQSPLSKLMKTMAADMKKARV
                     SVLEGKKPKNFGKHYDRIQTAAPSSEAKKGEHFQEYATSFLTQMERMQQASPETAKAE
                     FNALSKTCIRCHESYCPGPISMLRKLQVP"
     gene            complement(8466..>9278)
                     /locus_tag="PK281_05095"
     CDS             complement(8466..>9278)
                     /locus_tag="PK281_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48756.1"
                     /translation="LVCQDAVTLPLLEVSAGVKSRYQTQSQQCKPVQLINGLDLVSKA
                     EQGLKQCKNQRLNIELALLQLSLINAQQEEKKSPELSAAPAQDAVPKPNPTATPAPAE
                     KNPVLNFRGSVPLKTVSINQHLKKNEPVVKVESEEVKTHSVIEDLPRKHFELADLQAV
                     WKQYCDQLLASGRNSMQGTLTLHEPELGDNYQVVYRIHNDSQLRELELEKPDLVGFLR
                     KQLQNFNLQFTIEKTESNKSFRPFTPDEKFKAMEERTPALSILKAKIGLQPE"
     assembly_gap    9279..9378
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9379..10007)
                     /gene="dnaX"
                     /locus_tag="PK281_05100"
     CDS             complement(<9379..10007)
                     /gene="dnaX"
                     /locus_tag="PK281_05100"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014200936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HOY48757.1"
                     /translation="MENFIVSARKYRPVTFHSVVGQEAITGTLKNAIRNNQLAQAFLF
                     CGPRGVGKTTCARILAKTINCFNLSADTEPCNACESCTSFNQNASFNIHELDAASNNS
                     VDDIRALIDQVRFAPQVGKYSIYIIDEVHMLSTAAFNAFLKTLEEPPAHAIFILATTE
                     RHKILPTILSRCQVFTFSRIKVDDIARHLAYVASSEKIEAEPDALHVIA"
     gene            10190..10696
                     /locus_tag="PK281_05105"
     CDS             10190..10696
                     /locus_tag="PK281_05105"
                     /inference="COORDINATES: protein motif:HMM:NF012355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HOY48758.1"
                     /translation="MKNLSILALPIALLFASCGGNQPPADAPKTDTTSKTTPESSTPG
                     EAKLTIKVIGNSMADMGYEPTSSTVKAGDKVTLTLINTNTMEGMLHNWVLVKLGTGQE
                     VATAAIAAGMDKAYIPENPNVIAASPLAKPNETVTFEFTAPAAGQYNYICTFPGHFPK
                     MVGKLYVE"
     gene            10829..11152
                     /locus_tag="PK281_05110"
     CDS             10829..11152
                     /locus_tag="PK281_05110"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HOY48759.1"
                     /translation="MNFFSQFLERYPFIRNKYLLVFLFFLVWIVFFDKNNLISQYEDR
                     QALYELNKEKRYYQEEIENTHEQLNELMTDNRSLEKFAREKYKMKKANEEIWLVVDKS
                     KKPAQ"
BASE COUNT         3445 a         2197 c         2284 g         3311 t
ORIGIN      
        1 gatgccgatt tgctcaatcg aatggcaagt aaacaaggac ttttataccg aagtgccata
       61 atatcaaacg attattatgc actcaagcaa ttatatcagg aagattcctt gattaaagga
      121 aataccgaac tcgttaattg gcttttgtta aaaggcatct atgatctcca aaatattcga
      181 gatagcaagt tggaacagtc atacggactt ctcttaaaaa tgaaacgtga ttcgagttta
      241 tcgccaaaga ttcaactgga agtaaatcga atactagcaa cccttcaacc taaaataaaa
      301 ggtgagcctt ttcccgattt gggatgtcat gatcagccat attggaaaca acaaaagtgg
      361 aaaggattgc cagtatatct ggctatttta ccgaattatg cacctagttc acagttagtt
      421 atacgtcaat ttgctgcttt gcaaaccaag tatggtaaag aaatgcactt tgctctactc
      481 attcccgaag cagatgcgaa cgagatgaat atgctcaata agcagtttcc tgaattggat
      541 ttgggccaaa tggatttatg tcgtgaaaaa gtgcaagaaa ttattccgtc aaccagtcgc
      601 accaatttca ttttgataga tcgattgggt aatgttttcc aaaacccagc ggaaggacct
      661 gaaacagatg tagaatcggc gtttttagga ttaattaagc ttcactaatt gcttttcgag
      721 cttgaagaac atcaagtata gaagggtaag tattggcttc tacttcggtc cactggtcgg
      781 gtgatttcca aacggcttcg gtaatatctt cttcggtttg cgggatggtt ggccaagctt
      841 tgctggtttt aaaccaatac caaacggttt ccttaagtac ttcttttcct ttgtgaggat
      901 aagtatgcca agttcgacaa agttctgatt gaatgctcaa atcgagaatt cctgtttctt
      961 cttcaatttc acgttgagca gcgcttggta gatcttcacc cggttctact tttcctttag
     1021 gtaaatccca acaacctaat ctgaaaatga aaagtatttg gttatcagga gtttcgacaa
     1081 ttccaccagc agcttgaata aatgagtact tcgacttcaa ccattcggtt ccttgatcca
     1141 gatttatggc agttaaagaa agttgctgac attcattttt agctgatttt aatctttcaa
     1201 ctgcctcaaa cagctgtttt ttagaggctc ccgcatctat ccaaacagta tcaccttggg
     1261 gttgaatacc ttgcggattg ttgaaaaata aggcttttcg gttcaggtaa acggcgtatt
     1321 tttgcgacat gctttacaat actgatactg ccgcacagct ggcagatttt cttttacaaa
     1381 ttaaagccat aaagcttcag ccatccaagc cttttgtttg ggcttcgggt tggaaatctc
     1441 ctatttactg tgataatcgt ttgaccttgt catacccaaa ggttcgtacg ttcatccgtc
     1501 aggagttagc gaaggcaatt gccgataagt ttgagaagcc agatgtaatt gccggtgttg
     1561 ctacaggagg aattgcgcat ggtgctttgg ttgctcaaga attaggctta ccatttatat
     1621 atgtacgttc tgctcccaaa gggcacggaa tggaaaatct ggttgaaggt aaaattgaac
     1681 caggtcaaac agtagtggta gtggaagatt tgatttcaac tggacaaagc agtatcaagg
     1741 tcgttaaagc tcttcgtgat gcgggatgca tagttaaagg acttgtatct atcttcgatt
     1801 acggtttcga cgtagctgtt gctcagttta aggaagataa atgtccgttt tatagcctaa
     1861 gcaactacga tagtttactt gatcaggctg taaagaataa gtatgtagct ctggaagaaa
     1921 ttgatcagtt gaaaagctgg cgtgataatc cctccagttg gggagccaca ttgaaaattg
     1981 cttaacgcat gaaattagaa acatcttgga aacctgttca gacaacacct gatcaggttt
     2041 ttgctttcct atccgacatg aataacctga aaaagcttat gccagagcag gttataaact
     2101 ggacttctga tacagatacg tgtcagttca ccattaaagg gatggctgat ttaggaatga
     2161 aggtcgttga gcggaatgag catacatcta ttaagatgag ttcgcatgga aaagttccat
     2221 tcccatttac attaacggta ggaattcgtc ctttagatgc tatttctgaa gcacaattgg
     2281 tatttgatgg agaggtaaat ccctttttaa agatgatggt agaaaaacct ttagggaatt
     2341 ttttcaacat gcttttggat gggcttggac gccagttttg agtaattgca aggacttcca
     2401 cctacttcta gaacaccaaa cggaataagt cacaggcgat ttgttaaaat ctgttaaata
     2461 atctttggtt attccgaaaa atcagggcaa atttgcaccc aactatattt taacccacac
     2521 tcatgaaaaa cacactgaag ttagctgctg tcgctttgat cgcagtattc gttgttgctt
     2581 gctcaggcgg aaacggtcct gaaaccaatg caaaagctta tctagatgca ttaaatgcgg
     2641 aggatttcga caaagcaaaa gaatttgcta ctgaagacac aaaaggaatg cttgatttca
     2701 tgaaacaaat cgctacaatg ggcggtgaga agaaagaagc aaaagagaaa aaagaaatca
     2761 aagatttgaa atgtacagtt gaaggtgaca ctacagctac atgtacttac atggctggtg
     2821 acaaagaaga aaaactgaac atgaaaaaat caggagaaaa atggctagtt caccaaccaa
     2881 aagaaaaccc aatgggtggt ggtgaagaag gtggtgcaat ggaaggcgat atgggcacaa
     2941 cagatacgtc tgctactgct gctcctgctg ctgaagctgc tcctgctgct gaaagtcact
     3001 aatcattcct cttccgttac ggcatgcgca ggtttcggaa gatattaatc atttcaacgg
     3061 cccttttttt tgccggcata ggttttatct tgtataaagc cttttggagt ccagaaaaca
     3121 ctaaacaagt tttctacccc atgagccagc aggaaaaagg gcttttgcat tcaggagata
     3181 ttatccttcg tcgcggttat ggaatgttct ccgatggtat cgtcgaagtt cagaatagcc
     3241 ctgccgctgt atctcattgt gccatgatag ttggcgaacc cggtaaatgg agagttgccc
     3301 atgcactatc cagctctgtc gctgctattg atggtgctca agaacaatcc ttacaacgat
     3361 ttctcaacga aagtgtccca aatagcatta tcgtcaaacg attccgttgt caggaggata
     3421 ccattgcccg aattgtagat cgggtaagct actacacacg tatccacaaa ccgtttgacc
     3481 ataatttcga taaagtcgat actacccgat tctattgtac cgaattattt cagcattgtt
     3541 ttaaagatgt acttggtcga gacatctttt tgggtcaact cgattccaat acaacaggta
     3601 tttatgacct aagtaccttt caggatacct ctctttttga gacaattatt gatcatcata
     3661 cacactgaaa ggtatttttc ctaaaaaccg ttatttcatc cgtctcattt gttctatatt
     3721 cgaaaccgga aatcgagaat gaacatgaaa aaaattatca cgtccagtct gttattgctg
     3781 ggacttatat ctgccaaggc tcaaaatgaa gccgctattg cagcatataa tcaaggtatt
     3841 agcgagtata atgttaaaga tttccaaacg gctttgggaa gtttcaataa ggcaattgaa
     3901 tccgattcta cctttgggaa agcttggttt aaccgggctg tatgtaagtc ggacttgaaa
     3961 gactatgcag gagcgattac agattaccaa cgttccatga aaatagaagg cgaaaatgca
     4021 aagactcttt tctttatcgg acaagctcga tttcttagtg ctgataaaac gggtgcatta
     4081 gaagatttca agaaatcctc tcaactcgat cctaccaatg ctgaatcttt tttctatgcg
     4141 ggagggattt tatttgaaat agatgatttt caaggtgcaa cagaagaatt tactaaagca
     4201 ataaaagcaa gaagcgatta tgctgaagcc tataatgatc ggggtagctg taaacttaaa
     4261 ttagaagata ttaaaggtgc tattgatgat tatcgagaag caactaaact caatccaaaa
     4321 ttagcaattg cctataataa cttagcagca gctttacgtc gatcgggcga tttacaagca
     4381 tctcttcaag aatttaatta ttccattatt gccgatccta aaatggcaac ttcttatgtt
     4441 aaacgtggtc gtttgaaatt agaaatgcaa gatttagatg gagcaatgac tgatttcaac
     4501 aaggctattg aacttaatcc gaaagatcaa tatgctttga ataatcgtgg ttgtttgaaa
     4561 aataaactcc gcgactttcc cggagcaatt gccgatttcg atcaagctat tcgcatcaat
     4621 ccatcttatg gttatgctta tctcaataga ggaattgcca aagaaaattt acgggatctt
     4681 gccggcgcct gcaaagactg gtctgaagca tctcaaaatg gcgtagatgc tgccaaacaa
     4741 tatgttgact ctgaatgcaa ttgattacag acatgaaaaa aattctcctc ctagcccttt
     4801 tcatgctgcc tgttttcatt caagcgcagc agaatgtcga attcaccaag gcaaattttc
     4861 cggataacaa agccggtttg aaagaagcaa taaaaaacat tgagactggt gatagctatt
     4921 atttagctgc tgattatctc aaatatttag ctgttgattt ctacgttaaa gccaataatt
     4981 tcaatccgaa caatgctcta ttaaactttc gtttagggca ttcatacttg aattcaccta
     5041 acaaatacaa agcgctcgat ttcttaaaga aagcttacca aatgaatcct tccattgatc
     5101 cggaagtgac tttctactta ggtgaagctt atcacttgaa tcacgatttc gataatgcga
     5161 ttaagtacta ttctatgtac aagtcttcgg ctaccaaaaa gcctggattt gatcctgata
     5221 aacgtattac cgaatgtaac gaagggaaac gcttagttgc caacccagtt cgtgtaatga
     5281 tcgaaaatct tggaccagtt gtaaattcca aataccgcga atatgctgct gtaattactg
     5341 ccgatgagtc ggtcatgtta tttacttcac gaagagataa cactaccgga caagccatcg
     5401 ctgaagacgg tatgttttac gaagacattc tttccaccac caaacaaaat aaagcttgga
     5461 ctcctccggt aaacttaccc gctccggtta atactaagct tcacgatgct actatcgcct
     5521 tgtcggccga tggacagaaa ttgttcattt acacggatga aaatgaagga gatgttcttt
     5581 tatctaaact agaaggtgat aagtggtcaa aacccgaaca agctggtgga aatgctatta
     5641 ataccaaata ttccgagatt cacgcctgtt tttcttacga tgaaaaatca atctatttcg
     5701 tgagtaacaa accggaagat aattacggtg gatttgatat ttacgtaaca catcaagatg
     5761 ctaacggacg ttggggaaaa cccgaaaatc taggtcctgc tatcaatact atctacgatg
     5821 aagaaggagt atatcttcac cccgatggta aaaccatgta tttcagttct aaagggcata
     5881 aaaccatggg aagctacgat attttccgta gtgtttttga tgatggaaaa tggtctgagc
     5941 cagagaacat tggttatcct attaactcag ccgataaaga tgcctttttc gttgtatcag
     6001 catcaggtaa acatggttat tactcctctg ctcgtatgga tgctattgga gaaaccgata
     6061 tctatatgat tacgctactt ggtccagaga aagaaccaat cctgaacgtg gaagatcagc
     6121 tcttgcttag ccgtatgatg attactggtg aaaaggctcg tgctgaatcg gttgtagctt
     6181 ctaaaaccga taaaaataag ttccgttcag aggttaccat cctaaaagga actatcgtag
     6241 atgcattgga taaacatccg ttgaatgcca ttatcgtaat tactgataac gagaaaaacg
     6301 caatcgtttc tactttccaa gctaactcta aaacaggaca ataccttgtt tcgcttcctt
     6361 ccggacgaaa ttatggtatc gctgtaacca tggaagatta ccttttccat tctgaaaaca
     6421 tcgacttacc taaatctact gcttatcagg agatcgtgaa agatgttgag ttaagcaaat
     6481 tggagattgg taagaaaatc gtacttcgta atatcttcta cgatttcgat aaggcaacac
     6541 ttcgcccagg ttctatggct gagttggatc gtttagctaa acttcttgag caaaatccta
     6601 aaatccgtat tgagatttcc agccataccg ataacgtagg ttctgcagca tacaatcaaa
     6661 aactctctac caaccgagct ctttcggtag tggattactt agtcgacaaa aaaggcattt
     6721 caaagaaccg tctcgaattt aagggatacg gtttcgatga tccaattgct cctaacgaca
     6781 ccgatcaagg tcgacagtta aatcgtagaa ctgagtttaa gattctttcc aaataagttg
     6841 tattgcagct tcttttgggg gattaatcga acatgattca actgcttcct acctccgatg
     6901 gtagtacaac gctctatgat tccaacttgg atgaaaccta tcattcggtt catggagctt
     6961 tgcaggaatc gatgcatgtt tttattgaga aaggactaag acttcgatta cctgaacaaa
     7021 agcacattcg aattttagaa attggtttcg gaactggctt aaatgctttg ctaagtcttc
     7081 gagaaacgaa actcgaccca actttaacgg ttgactattg gtgtttggaa ccatttccaa
     7141 tttcaaagtc gatttacttg caattgaatt acacgcaacc cgaagagcaa gcgaattttt
     7201 tggcctttca tcaagccgag gcaggaaggt ctatttctat caatagtagc tttactttta
     7261 ggcgtttaga acaaagcata gacggattta cctcagaaca aatgttcgat atggtttttt
     7321 acgatgcttt tggtccgcct tctcaacccg aattatggac tactgaaacg ttgaaataca
     7381 gcgttgatgt gctttcacct ggcggaattt gggtaagtta ctgtgctaaa ggtgatgtgc
     7441 gaagaagttt aatttccttg ggtttgcagg caaaacgact acctggtcca ccaggaaaac
     7501 gtcacatgct tttcgcacaa aaaggaaacg tgtaaattcc taaattgcag caccaaaatt
     7561 aaactatgaa aaagacactt acattcctga tgttgcttgt ttttggagct attcaggtga
     7621 acgcgcaaaa agcagaatat ttcggcgaaa aaatcaaaaa agacggagcg atttctaccg
     7681 ccgagttttt gaaaatgatg gaaggcaaag attctcttgt cgttaaaatt gaagctccaa
     7741 tcaacgaagc ttgtcagaaa aaaggctgct ggatgaatgt tgatttaggt ggcggaaaag
     7801 aaatgatggt tcgctttaaa gattacggtt tcttcgttcc gaaagattgt aatggaaaga
     7861 aagcaatcat gaatggaatt gcctatcgtg aagttgttag cgttgaaatg cttcgtcact
     7921 atgccgaaga tgcaggcaaa tcgaaagaag aaattatgaa aatcactcag cccgaaacga
     7981 aattatcttt cgaagcggat ggtgtaatca tttacgcggt taaataagta tgtaccggat
     8041 tgcttttctc attgctatag ttagcatgtg ttttatctcg gcttctgcag ttaaacaaac
     8101 gcaaagtcct ttgagtaaac tcatgaaaac gatggctgcc gatatgaaaa aggcgcgtgt
     8161 ttctgtgttg gaaggcaaaa agcctaaaaa cttcggtaaa cattacgacc gcatccaaac
     8221 agcagctcca agtagtgaag caaaaaaagg agaacatttt caggaatacg ctacttcatt
     8281 tttgactcaa atggaaagaa tgcaacaagc tagtcccgaa acggctaaag cagaattcaa
     8341 tgcgctaagt aaaacctgta tacgctgcca cgaaagttat tgtccagggc ctattagtat
     8401 gctgagaaaa ttacaagtac cttaaacaaa acgcccggta gattattctt ccgggcgttt
     8461 ttattttatt caggttgtaa tccgattttt gctttgagta tgcttaatgc gggtgttcgt
     8521 tcttccatgg ctttaaactt ttcgtctggg gtaaaaggcc gaaacgattt attggattct
     8581 gtcttttcga tagtaaactg gagattaaag ttttgcaatt gtttcctaag aaaaccaact
     8641 aaatcgggct tctccaattc taattcacga agctgactgt cgttgtgaat ccgataaaca
     8701 acttgataat tatctcctaa ttcgggctcg tgaagtgtaa gcgttccttg catggaattt
     8761 cgtcccgaag caagtaactg atcacagtat tgtttccaaa cagcttgtag atcggctaat
     8821 tcaaaatgtt tgcgaggtaa atcttcgata accgaatgag tttttacttc ttcgctttcg
     8881 actttaacaa ccggctcatt tttctttaag tgctgattga tcgacaccgt tttaagcggc
     8941 acacttcccc gaaaatttaa taccggattc ttttctgcag gcgcaggtgt tgctgttgga
     9001 tttggcttag gtacagcgtc ttgcgctggt gcagcactta actcaggact ttttttttct
     9061 tcctgttgag cgttaatcaa gcttaactga agcaaggcca attcgatgtt taggcgctga
     9121 tttttacact gctttaagcc ttgttcagct ttactaacta aatcgagccc gttgattaat
     9181 tgtacaggct tacattgctg actttgcgtt tgatatctgc tttttacacc agcacttact
     9241 tctaaaagtg ggagcgtaac agcatcctga caaaccaann nnnnnnnnnn nnnnnnnnnn
     9301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9361 nnnnnnnnnn nnnnnnnntg agctatcacg tgaagtgcat ctggttctgc ttcaattttc
     9421 tcgctactgg ctacatatgc taagtgacga gcaatatcat ccaccttaat tcgactaaac
     9481 gtaaatacct gacaacgaga aaggatcgtt gggagaattt tatgacgctc cgttgtagct
     9541 aaaatgaaaa tcgcatgagc aggaggttct tctaatgttt taagaaatgc gttaaatgcc
     9601 gcagtactga gcatgtgaac ctcatcgatg atgtagatgg aatatttccc aacctgtggt
     9661 gcaaatcgaa cttgatcaat caaagcacga atatcatcca ccgaattgtt ggaagcagca
     9721 tctaattcat gaatgttgaa tgacgcattc tgattaaatg aagtacaact ctcacaggca
     9781 ttacacggtt ctgtatcagc cgataaattg aaacaattaa tcgtttttgc tagaattcgg
     9841 gcacaggtgg tttttccaac tcctcgcggt ccgcaaaaca gaaatgcttg tgcaagttga
     9901 ttattacgga tggcattttt taatgtaccc gtaatagcct cttgtccaac caccgaatga
     9961 aaggtgacgg ggcgatattt ccgggccgag acgatgaaat tttccattgt acaaatataa
    10021 cccaagccta agcctagaca cacggtgtta acatctttta gaaatttgtc ttttgagcgc
    10081 tattcatctt caaaaggact atttgttggt tgattttttt gaaatctaag tcacattctg
    10141 acaaacaatt cacccgaaac tttgtcttgt tatctaacaa tttcatgtaa tgaaaaactt
    10201 aagtattctc gcactcccaa ttgccttgtt atttgcatcc tgtggcggaa atcaaccacc
    10261 tgctgatgca cctaaaacag atacaacttc caaaacaact cccgaatctt ccactccagg
    10321 tgaagccaaa ctaacgatta aagtcattgg taattcaatg gctgatatgg gctatgagcc
    10381 aacttcttca acagtaaaag cgggcgataa agtaacactc acgctcatca acaccaacac
    10441 catggaaggc atgttacata attgggtttt ggtgaaatta ggaactggtc aggaagtagc
    10501 tactgctgct atcgctgctg gaatggacaa agcatacatt ccagaaaatc caaatgttat
    10561 agctgcttcg cctttggcta aaccaaacga aacggtaacg ttcgaattta ctgcgcctgc
    10621 tgcaggacaa tacaactaca tctgcacatt cccagggcat ttccctaaaa tggtagggaa
    10681 attatatgtt gaataaatcg gtgtaatttc tttggaaagc ctctgttctt aattggatag
    10741 aggctttttt gctaaaatca acaagatggg tctgacaact tttcgatctt tttcacttac
    10801 atgtctgatg cacgcatatt tttggaggat gaatttcttt agccaattcc tcgaacgata
    10861 tccgttcatc cgcaacaaat acttgctggt gttcttgttc ttcttggttt ggattgtctt
    10921 ttttgataaa aacaacctta tttctcaata cgaagaccgt caggcacttt acgaattgaa
    10981 taaggagaaa cgttactatc aagaggaaat tgaaaacacc catgagcagt taaacgaact
    11041 catgaccgac aaccggagcc tcgaaaaatt cgcacgtgag aagtataaaa tgaagaaagc
    11101 gaatgaggaa atttggttgg tggtggataa atctaaaaag cctgcacaat aaggcagcaa
    11161 gatttttagg gattgaattt gctacttttg ttcttcaagc ctgaagcctt tgaaaccgct
    11221 attctctgaa ttttctgcta ctaattttga ccaatggaaa gctcgacttc tccaggatct
    11281 caaggtcgat tccgttgata agtacctgag cacccgaatc gaagaactgg aaacccc
//
LOCUS       DAOOQW010000057        11315 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_7185_length_11315_cov_4.574156, whole genome shotgun sequence.
ACCESSION   DAOOQW010000057 DAOOQW010000000
VERSION     DAOOQW010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 11315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11315
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7185_length_11315_cov_4.574156"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..614)
                     /gene="thiL"
                     /locus_tag="PK281_05115"
     CDS             complement(<1..614)
                     /gene="thiL"
                     /locus_tag="PK281_05115"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004569451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HOY48760.1"
                     /translation="MSANRSEINQLGEFGLIDRISTKFSLTNSTSIKGIGDDAAVIDA
                     GDDFVLISTDMLVEGIHFDLSYMPIQHLGYKAVAVNVSDIAAMNGKAEQITVSIALSN
                     RFSLEAIDALYAGIKSACESYGADLVGGDTTASVSGLIISISVVGRVKKDKVSYRSGA
                     KPTDIICVTGDLGAAFMGLQILEREKEVFLTNPDMQPGIEQYEYL"
     gene            743..907
                     /locus_tag="PK281_05120"
     CDS             743..907
                     /locus_tag="PK281_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48761.1"
                     /translation="MKKIIGLFLAAGILLSACGPKPYYKTAKGKKKTKYYNEIQFGGK
                     SASEMKKPKN"
     gene            complement(952..1350)
                     /gene="mce"
                     /locus_tag="PK281_05125"
     CDS             complement(952..1350)
                     /gene="mce"
                     /locus_tag="PK281_05125"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019386910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HOY48762.1"
                     /translation="MEKLEHIGIAVKSIEESNVVFAKLLGREHYKIEKVESEGVTTSF
                     FEVSGVKIELLEATRPDSPIAKFIEKRGEGIHHLAFAVADIDASIEEYKKKGFEPINQ
                     EPKKGADNKMICFLHPKKSGGVLIELCADI"
     gene            1422..1835
                     /locus_tag="PK281_05130"
     CDS             1422..1835
                     /locus_tag="PK281_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48763.1"
                     /translation="MMIETLSDMHVEAARIQQEIKDYLGLRTSDLVFEFTSIDGKVKL
                     DLITINPRHNQAFLFHNETGADKVDALKKMLAYVQDYKEKENSYTIQWVAKSDKELHT
                     SYFKASNIMDALQKLYYGRDMNTITVFSVVLNPIS"
     gene            1840..2166
                     /locus_tag="PK281_05135"
     CDS             1840..2166
                     /locus_tag="PK281_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007646870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HOY48764.1"
                     /translation="MINVTEKAKDRIIQLRREEGKAVENNVRVLVKGGGCSGLMYDLN
                     FDDEIKSADQIFEDKGIKILVDKKSLLYLLGTTLDFSDGLNGKGFQFINPNATRTCGC
                     GESFAV"
     gene            complement(2175..2549)
                     /locus_tag="PK281_05140"
     CDS             complement(2175..2549)
                     /locus_tag="PK281_05140"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019387571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HOY48765.1"
                     /translation="MKNKKFSTPQRLKSFVYAFNGLKILIREEHNSRIHFVIAIVVTI
                     AGFILEISVEEWIAIVIAIGFVIAAEIFNSSIENIADFISPEKHEKIKKIKDLAAAGV
                     LISALTALTIGLIIFLPKIIEF"
     gene            complement(2673..3662)
                     /locus_tag="PK281_05145"
     CDS             complement(2673..3662)
                     /locus_tag="PK281_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006987215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOY48766.1"
                     /translation="MKKKIRWGILGCGKIANKFAADLQWVEDAELVAIASREVEKGAA
                     FAKQYNAPLNFNTYEGLASCKDVDVIYIATPHGFHHEHTLLCLQHKKAVLCEKALALN
                     TKQVTEMINFAREQKVFLMEAFWTKFLPQYQKVSSMISAGEIGTIKMIEADFGFKATD
                     PTPQRMYDPVLGGGALLDVGIYPVFLALSFLGKPLDVQAFITPYDTGVDEQISMTMKF
                     KEGALAVLNASFAADTPVEATLVGTEGYIRISNRFHNATSKIELIKDKQSPVEIQVHR
                     EEGYGYQFEARHVGECLRTGLTESPIMSHTDSLLLMETLDRVRANCGIKYEVD"
     gene            complement(3684..4541)
                     /locus_tag="PK281_05150"
     CDS             complement(3684..4541)
                     /locus_tag="PK281_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOY48767.1"
                     /translation="MRNVRLVLGSGGARGMAHIGVIEELEKEGFSIKEVVGCSMGAVV
                     GGIYCAGYLQEYKHWLIGLSRLDVFRLLDFAFSSQGFVKGERVFKEIEKLIGDHTIEN
                     FQIPFTAVAADMMKKREVHYTSGSLFKALRASIAIPTVFTPVMEGRSQLVDGGVLNPL
                     PLNVIHPEEGDVVVAVNLNANTPVEANSPVVEEGKEMAAYQKMLIALLPQFLSSENRV
                     EQFGFFDLLNKSFDMTQDRLTELTIDVHKPDIVVDISRDACGVFEFYRANEIIEQGRK
                     AFQKAYTKI"
     gene            complement(4980..>11315)
                     /locus_tag="PK281_05155"
     CDS             complement(4980..>11315)
                     /locus_tag="PK281_05155"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HOY48768.1"
                     /translation="TSPLDSGNGSASAYDIRYSTSPITDENFGDATQYLNPPAPQPAG
                     ATESLTISGLLPGMVYYFAIKSADFFGNFSSVSNILQQATNFAPVITLSATSLTQDLQ
                     TALTGSQILTISNTGLGVLNFALSVPSGFATASPLSGTVAPGASLDVTITFDANNLLS
                     GSYNQTLDISHNDPLQDSTSVHLTLNVTNNGFPIASVEPDSLDFGNVFETDFASQFVY
                     VHNAGSEPLTITDIASDNTDFTAEADSLFVVPFGNAAIKVTYTASSLGTSSGNIIILT
                     NDGINPSLVVSVKGTGVVAPEIVVSPDSLSASLNTGKSSTQILTIGNTGGSNLDFSIA
                     VKSSAPASVASVQTVSVPFKSASSVAGTQKQTTKSYGKYSNQIKLKSVGKSAVASKVL
                     IISPDDDMTDLAFVLNSFDDIDATILSGGSLPGLSLDTLMQYDVVLTSNNTQWLASGG
                     IDPALIGDLLADYIDAGGKVIVNQFAYSYDAWQMAGRFIDDGYGPFVPSTTDAVVDVE
                     LGTILAPGHPIMQGVSSLFYSGYVQNVGLSPGSIALAEWNDGELFLAVNSNVVALNML
                     PSLGNGDPLQWSGDLPTIFQNAVHYLSGGGSFVSVSPSEGTIAPGTQLDLEVTFDASG
                     LDAGIYNALVDISTNVPYHQLVSIPAELTVLGPEFTISPDSLYDELENQQTSTHTLVL
                     SNNGPDDHSFTVAVQGSGFSTSSVKIIKPQSAGRTATANPAKQKQQKSARKNNDRDDI
                     HVISNTTETAKSAGRIATVAQSSVGLYATDFEDLSIGDINGQNGWFGQWVNWTVEAEN
                     PYGGSQHFRGLSDGFGFTASISPSGPIGADPISTVSMKVNLQGSNVTWQIAPQSPSAG
                     LVVTRIGFEADGSMTALVSDGAGGGFFQTIAPSVPTGYFDLTISASRDSATFDVFIDD
                     AKIFSGLGFAGDIEEVAILSWMEEAGPTLDIDDFQIIDGVKEDAPTFVTVSPLSGNLP
                     SGATAEITVNFASEDLTFGLYSTNINIDIDGSKLTVPASLRVVGDEAIDVSPTVLQSV
                     LDYKADTTMVFNIANTGGRPLSYGIQVIGASTDMANLPKSPIGKFANWTTDKRIVDKK
                     AKDDSASKSITSKSAKVELLAGSALLEENFEGGTFPPAGWSTIDNEGEGVVWGFAEDV
                     GEGNYSGVGEAATISSDVFGPAEFDAELISPSVNIAGLKNIVVQYNVNYSNYANLDFL
                     DLDIQVDGGAWTNVLSWNEDHGTFRGTPGEFVSVSLDAYLSGASSFRLRWHYYDPNTG
                     DWDWYAQIDDVAILGDARAWLSVAPASGIIPVGESVDISAKFDASDIEPGFYVAGILV
                     TSNAVQDPLVGVVASLDVRDPAVISVDPDSFYVELPDQLTTQRILTVSNSGESALQYS
                     VTEVAIPTSTGIVQARRESSEKRTTPSQQTLNLFDTKAIQNSIAKHAGMDIYSTGFEE
                     FATGDINGQNNWVGQYSNWTIESYNPASGSQHFSGLADGLGQSLAFSPLVLPGSDDVS
                     TVSMKLDLDPGVTWQIIPQSPTAGSVNTRFQIAPDGSLSALVQDSVGNVYYQPIAGTL
                     PDGYFDFRIENKASTHQFTVYVNGSPIFTGGGFADEIEQVVIFSLMEDAGQLLDIDNF
                     YILDGPAQAPWLTVDPKSGSAASGSSSIVNLYFDATDVEPGTYSKILNISSNDPATPN
                     VSVPVTLVVLPNLPPVLATAYDKAVRELGTLNLSFTATDADDSVVVVRLENAPDFITM
                     TNMQNGLVSYSAKPLIGDAGDYEITVVAEDGRGGIATENFNLKVTPYGVQNFSLINFQ
                     TGAVLQNFEDTVTIDIADPLISKYTIRANTNPGTVGSVKFTLDGSNTNTDNSRYYTIN
                     AWLIPVLSGGDHSITAQGFTRSNGNGDGGQVKMGVIRVINSVAVTDFDVVSGSGTKLL
                     DLEDGGVIDISQSGFNKINIVAYTGSSTIRSVKFTLNGTIARIDNGSPFALKGNADGS
                     DTFWPASPGSYTLTATPYLSYHAWGPAGTPLTIHFQVINGEDPTVANARSGSASSENE
                     REEMQVQEFGEYSWTIYPVPVIDDLHIKLEGKVEGNVSVNIINTQGQSVHLKDGTAET
                     FRDYTVSTVKLGLTTGFYLIQIQQADGKRITKKFMKE"
BASE COUNT         3407 a         2474 c         2078 g         3356 t
ORIGIN      
        1 aggtattcat attgttcaat accaggttgc atatctggat ttgttaagaa cacttctttc
       61 tcgcgttcta aaatttgcaa gcccatgaaa gccgcaccta aatcgcctgt aacacaaata
      121 atatccgttg gctttgctcc actgcgatat gataccttat ctttttttac acgacccaca
      181 actgagatcg aaataattaa acccgaaaca gaagcagttg tatcgccacc aactagatct
      241 gcaccataac tttcacaggc gcttttaata ccggcataca acgcatcgat cgcttcaagt
      301 gaaaagcgat tactcaaagc aatgctcact gtaatctgct cagccttccc attcatggcg
      361 gcaatgtctg atacatttac agccacagcc ttatatccca aatgttgaat gggcatgtac
      421 gacaaatcaa aatgaatgcc ttccaccagc atatccgtag agatcaacac aaaatcatca
      481 ccggcatcaa tcacggctgc atcatcgcca atacctttta tggatgttga atttgtgagg
      541 ctgaatttcg tgctgatccg atcaatcagt ccaaattccc caagttgatt aatctccgat
      601 ctatttgcgc tcatataaca atggtttaac ttgcccttca ccgatttgaa ttgggcttta
      661 tcctgcaaaa gtaagattat gaacgaatat ggaaggtttt gttcgaaata agctggtatc
      721 tatgcatttg aaatcattaa ccatgaaaaa gattattgga ctcttccttg cagcgggcat
      781 cttacttagc gcatgtgggc ccaagcccta ttataaaact gcgaagggta agaagaaaac
      841 gaagtattac aacgaaattc agttcggtgg caaaagcgcc agcgaaatga aaaaacctaa
      901 gaattaaaat tcttccctaa atcccgctaa aatcgggagg cataggattt tttaaatatc
      961 agcacacagt tcgattaaaa cacccccact ttttttaggg tgcagaaaac aaatcatttt
     1021 gttatctgca cctttttttg gctcttgatt gatcggttca aatccttttt tcttgtattc
     1081 ttcaatgctg gcgtcaatat cagcaacagc aaaggcaagg tggtgaatac cttctccccg
     1141 cttctcaatg aacttagcaa tgggcgaatc tggacgtgtg gcctccaaca attcgatctt
     1201 tacacccgaa acttcgaaaa aggaagtcgt taccccttcc gattctacct tttcaatttt
     1261 ataatgttca cgccccaata gcttggcaaa gactacattt gactcttcaa tgcttttaac
     1321 cgctattccg atatgctcaa gtttttccat aattgctacc tttgcaggca aagaagaaac
     1381 tttttggcta aatcgaaagt ttagcataat gaaaattact tatgatgatc gaaactttga
     1441 gcgatatgca cgttgaggcg gctcgtattc agcaggaaat taaggactac ctgggcctgc
     1501 gtacctccga ccttgttttt gaatttacct ctatcgatgg caaggtaaag ctcgatctca
     1561 ttaccattaa tccacgacac aatcaggctt tcttgtttca caatgaaacg ggtgccgata
     1621 aagtagacgc attgaaaaag atgctcgctt atgttcagga ctacaaggaa aaagaaaatt
     1681 catacaccat acaatgggta gcgaagagtg acaaagaact tcacacttcg tactttaagg
     1741 caagcaacat tatggatgcc ttgcaaaaat tatattacgg acgcgacatg aatacgatta
     1801 ccgttttcag tgttgtatta aatccaattt cataaagcta tgattaacgt aactgaaaaa
     1861 gcaaaagata gaatcattca acttcgcaga gaggaaggta aggccgttga aaacaatgta
     1921 cgcgttttgg tgaaaggtgg cggctgctct ggtttgatgt atgatttgaa ttttgatgac
     1981 gaaataaaat cagctgatca gatttttgaa gataagggta ttaaaattct ggtcgacaaa
     2041 aaaagcttgc tgtatttgct cggtactaca ttagatttct ctgatggatt aaacggaaaa
     2101 ggtttccaat tcattaatcc aaatgctacg cgcacatgtg gatgtgggga gagttttgcg
     2161 gtttagtaca gaatttagaa ttcaataatc tttggtagaa aaataattaa tccaatggta
     2221 agcgctgtta atgcacttat taaaactccg gctgcagcca aatcttttat cttcttaatc
     2281 ttctcgtgtt tttctggcga gataaagtcg gctatatttt cgatagaaga attgaaaatt
     2341 tccgctgcga taacaaaccc aatggcaatt acaatggcaa tccattcttc tactgatatc
     2401 tctaatataa atccagcaat cgtaacaact attgctatta caaaatgaat tcttgaattg
     2461 tgctcttccc ttatcagaat tttcaatcca ttaaaagcat aaacaaaact ctttagtcgt
     2521 tgtggggttg agaatttttt atttttcatt gctaagccat tcaatccaat tccaatctcg
     2581 gattacctat cactattgtt tggtctcagg atttatttgt tcctcttcac atcgctttca
     2641 ttcaacctac accagtgtca ataaatccct tttcaatcaa cttcgtactt aataccgcag
     2701 tttgcgcgta cacgatcaag cgtctccatt aaaagcagag aatcagtatg actcattatc
     2761 ggactttccg ttaaacctgt tctcaaacat tctcccacgt gacgcgcctc aaactgatag
     2821 ccataacctt cttcccgatg cacttgaatt tcaactggag actgcttatc ctttatcaat
     2881 tcaatctttg acgttgcatt atggaaccga tttgaaatac ggatgtaacc ctccgtgcca
     2941 acaagggttg cttctaccgg agtatcggca gcaaaacttg cattcaatac cgctaaagct
     3001 ccctctttaa atttcatggt catggaaatc tgctcatcta caccagtatc gtaaggagta
     3061 atgaacgcct gtacatccag tggctttcct aaaaaagaga gcgcaagaaa taccggataa
     3121 attccaacgt ctaacaacgc gcctcctcct aaaacgggat cgtacatacg ttggggtgtt
     3181 ggatcggttg ccttaaatcc aaagtcagct tcaatcattt ttatcgttcc tatttcaccc
     3241 gcagaaatca tggaggatac tttttgatat tgcggtaaaa acttggtcca aaatgcttcc
     3301 atcaaaaata ccttttgctc acgggcaaag tttatcattt cagttacttg cttcgtattt
     3361 aaagccaatg ccttttcgca gagcactgct tttttatgtt gaagacagag caaggtatgt
     3421 tcatggtgaa atccgtgcgg tgtagcaatg tagatcacat cgacatcctt gcaagatgcc
     3481 aatccttcat atgtgttaaa atttaatgga gcattatact gttttgcgaa cgcagctcct
     3541 ttttccacct cgcgtgatgc tatggccaca agttctgcat cttcaaccca ttgcagatca
     3601 gccgcaaact tattggcaat ttttccgcag cccaaaattc cccatctgat tttctttttc
     3661 atctacctgt tgtttatgct acattagatt ttagtgtatg ctttttgaaa agcctttcta
     3721 ccctgctcaa taatttcgtt tgcacgataa aattcaaaca caccacatgc gtctcgcgat
     3781 atgtccacaa cgatgtcggg tttatgaaca tcgattgtta attctgtaag gcggtcttgt
     3841 gtcatgtcaa acgacttgtt gagtaaatca aagaagccga attgctcaac tctattttca
     3901 gagcttaaaa actgaggcaa caaagcaatc aacatttttt gataggcagc catttctttt
     3961 ccttcctcaa caacaggaga atttgcttcc actggagtat tggcattgag gtttaccgct
     4021 acaaccacat caccctcttc cggatggatt acattcaatg ggagtggatt taaaacacca
     4081 ccatccacta attgtgatcg tccttccatt acgggagtaa atacagttgg tatagcaatg
     4141 gacgcgcgca acgctttaaa taaactaccg gatgtgtagt gaacttcacg tttcttcatc
     4201 atatctgccg ctactgcagt aaaaggtatt tggaaatttt caatagtatg atctccaatt
     4261 aatttttcga tttctttaaa aacacgttcg ccctttacaa agccttgaga tgaaaatgca
     4321 aaatcgagca gcctgaacac atccaatcgt gaaaggccaa tgagccaatg cttatactcc
     4381 tgtaaatacc ctgcacaata aatacctcca accacagcac ccattgaaca tcccacaact
     4441 tctttgatgg aaaatccttc tttctctaat tcttcaatta cgccaatatg cgccatacct
     4501 cgtgctcctc cactacctaa taccagtctg acgttcctca ttaccacaga ttattcttaa
     4561 agatatagaa cagaatatta cgaatatatt ttattcctgg cttcacgagg gaaatcgtct
     4621 ttaaagagtt tgagatgctt tcctgttagc actttttttg ttgttgaacc cgtacacaaa
     4681 caggtgactc ctctggagtc aaataaacta acaaataaat tctacaaaca gattgctcct
     4741 ccggagcagc gagcagcaaa cgattaaaaa ttatcaaatc aatttaaaac aatcaataat
     4801 tataaagtta aaaggcgatt ttcctgatgt cttcacggat accttagaaa ctcgcatagt
     4861 atgatcggaa aataatcacc tcatgttgat acctgttgat tattctatat ctcaaatatt
     4921 gggcaataaa aaagggtgag gttaacaacc ccaccctctt aatcatttac tattattact
     4981 tactccttca taaacttttt ggtaatgcgt ttaccatcag cctgctgaat ttgtattaaa
     5041 tagaaacctg ttgttaagcc gagtttaact gtactgacag tatagtcacg gaaagtttct
     5101 gctgttccat cttttaaatg aacggattgc ccttgtgtat tgataatgtt tacggataca
     5161 tttccttcca ctttaccttc gagcttgatg tgaagatcat ctattacagg tactgggtat
     5221 atcgtccatg aatattcccc aaattcctga acttgcattt cttccctctc attctcggat
     5281 gaagcactac ctgatcgcgc attagcaacc gttggatctt ctccattaat aacctgaaaa
     5341 tgaattgtaa gaggagttcc tgctggtccc catgcatggt aactcaagta tggagttgct
     5401 gttaatgtat agcttccagg acttgccggc cagaaggtat cgcttccgtc tgcattacct
     5461 tttaaggcaa atggcgaacc gttatcaatt cgtgctatag ttccgttgag cgtaaactta
     5521 acacttcgta tagtgctgct acccgtatag gctacaatat taatcttgtt gaatccagat
     5581 tgactaatat caattactcc tccatcttca agatcaagca acttcgtgcc gcttccactc
     5641 acaacatcaa aatcagttac cgctacagaa ttaattactc taatgacacc cattttaacc
     5701 tgacctccgt caccatttcc gttgcttcgt gtaaagcctt gcgcagtaat ggaatgatcg
     5761 cctccactca atacaggtat tagccatgcg ttaatggtgt aatatcgaga gttgtcagta
     5821 tttgtattcg aaccatccaa tgtaaacttc acacttccaa cagtgcctgg atttgtattg
     5881 gcacgaatcg tatattttga aatcagcgga tcggcaatat caattgttac tgtgtcttca
     5941 aaattctgaa ggactgctcc tgtctggaag tttatgagac tgaaattttg aacaccatat
     6001 ggtgttacct ttaagttaaa gttttcagtt gcaatacctc cgcgaccgtc ttcagcaaca
     6061 actgtaatct cataatcacc agcatcgcct attaatggtt ttgctgaata cgaaaccaat
     6121 ccgttttgca tatttgtcat cgtgatgaaa tcaggagcgt tctccaatcg cacaacaaca
     6181 actgaatcat cagcatctgt ggccgtaaag gaaaggttca acgttccaag ctcgcgaact
     6241 gctttgtcat atgcagttgc taataccggt ggaagatttg gtaaaacgac aagcgtaact
     6301 ggcaccgata catttggtgt tgccggatca ttgcttgaaa tatttaaaat cttactatag
     6361 gttccaggct caacatccgt tgcatcgaaa tacaaattaa ctattgatga acttccagaa
     6421 gctgcgctac ccgatttagg atcaaccgtt agccatggtg cctgagcagg accatccaga
     6481 atataaaagt tatcaatatc caaaagttgt ccggcatcct ccattaatga gaagataaca
     6541 acttgctcta tctcatcagc gaatccgcca cccgtaaata ttggtgagcc atttacataa
     6601 acagtaaatt gatgcgtact cgctttattc tcaattctaa aatcaaaata tccatcagga
     6661 agtgttccag cgattggttg ataataaaca tttccgacac tgtcttgaac aagagcactc
     6721 aaacttccgt ctggagcaat ttgaaaacgg gtgttaacgc ttcctgctgt aggcgattga
     6781 ggaatgatct gccatgtaac accaggatca agatctaatt tcatagagac cgttgataca
     6841 tcatcgctac ctggtaaaac aagtggtgag aatgcaagtg attgaccaag tccatccgct
     6901 agtccactaa aatgttgtga tcccgatgct gggttataac tctctatagt ccagttgctg
     6961 tattgaccaa cccaattatt ctgaccatta atatcgcctg ttgcaaactc ttcaaagcct
     7021 gtagaataga tatccatgcc tgcatgtttc gctattgaat tctgaatggc tttggtatca
     7081 aataaattta aagtttgctg cgaaggcgta gtgcgtttct ctgaagattc tcttctcgcc
     7141 tgtacaattc cagttgaggt tggtattgcc acttcggtaa cagaatattg caaagcgctt
     7201 tcaccagaat ttgaaacggt aagaatacgt tgcgtagtta attggtctgg caattcgaca
     7261 tagaatgaat ccggatcaac agaaattact gcaggatctc gtacatccaa agaagcaaca
     7321 acaccaacca atggatcctg aactgcattg cttgttacca gaatacctgc tacatagaat
     7381 ccaggttcga tatcggatgc atcaaatttt gcagaaatgt caacgctttc tccaaccgga
     7441 attatacccg atgcaggagc tactgataac catgcgcgtg catctcctaa aattgctaca
     7501 tcatcaatct gtgcatacca atcccaatca cctgtattag gatcataata atgccaacgt
     7561 aaacggaatg atgaggctcc tgataaataa gcatcaagag atactgaaac aaattcaccc
     7621 ggtgtacctc taaaggtacc atggtcttca ttccaactta atacattcgt ccatgcacca
     7681 ccgtcaacct gaatatcaag atcgagaaaa tccaggttgg catagtttga gtaattcaca
     7741 ttatactgaa ctactatatt ttttaatcct gctatgttta ccgatggcga aattaattca
     7801 gcatcaaatt ctgcagggcc aaaaacatcg ctgctaattg ttgctgcttc tccaacacct
     7861 gaataatttc cttcaccaac atcttctgca aaaccccaaa ccacaccttc gccttcattg
     7921 tcgattgtgc tccatcccgc aggcggaaaa gttcctccct caaaattctc ttcaagcaat
     7981 gcacttcctg caaggagttc aacttttgct gatttagatg ttattgattt agatgcgcta
     8041 tcgtcttttg cttttttgtc gacaatgcgt ttgtctgttg tccagtttgc aaatttacct
     8101 atcgggctct taggtagatt agccatatct gtacttgctc ctattacctg aattccataa
     8161 ctcaatggcc tgccgcccgt gttcgcaatg ttaaacacca tggtggtatc ggctttgtaa
     8221 tcaagaacag actgaagtac tgtaggacta acatcgattg cttcatctcc tacaactcga
     8281 agtgatgcgg gaaccgttaa tttagaacca tcaatgtcta tattaatatt tgttgaatac
     8341 aatccgaagg taagatcctc agatgcaaaa ttcacggtga tttccgcagt tgcaccagaa
     8401 ggtaaatttc cggaaagagg tgaaaccgtt acaaaggttg gtgcatcctc tttaacacca
     8461 tcgataatct gaaaatcatc aatgtctaat gttggaccag cttcttccat ccatgaaaga
     8521 atagctactt cttcaatgtc tccggcaaaa ccaagaccac taaagatttt agcatcatca
     8581 atgaacacat caaaagtcgc tgaatctctg ctggctgata ttgttaaatc aaagtagcca
     8641 gtaggaacac ttggtgctat tgtttgaaag aaacctcctc ctgcaccatc actcaccaaa
     8701 gccgtcatgc taccatcagc ttcgaaacca atacgagtaa caaccagtcc ggctgatggc
     8761 gattgaggag caatctgcca agttacattt gatccttgaa gatttacttt cattgataca
     8821 gtagaaatcg gatctgctcc gatcggccca gatggagaaa tggaagctgt gaacccaaat
     8881 ccatctgata aacctctgaa atgttgagag ccaccatacg gattttctgc ttcaacagtc
     8941 cagttaaccc actggccaaa ccatccattt tgaccattga tatctcctat tgataaatct
     9001 tcaaaatccg ttgcatataa tccaacagaa ctttgtgcaa ctgttgctat tcgaccagca
     9061 ctttttgctg tttctgttgt atttgaaatc acatgaatat catcacgatc attattctta
     9121 cgagcagact tctgttgttt ttgtttcgca ggattagctg tagcagtacg cccggcagat
     9181 tgaggtttta ttattttcac tgacgatgtt gaaaaacctg aaccctgaac agcgacagta
     9241 aacgaatgat catccggacc attgttgctc aatactaatg tatgtgttga tgtttgttgg
     9301 ttttctaatt catcataaag tgaatcagga cttattgtaa attccggacc aagaacggta
     9361 agctcagcag gtattgatac tagttgatga tatggaacat ttgtcgagat gtcaactaaa
     9421 gcattataaa taccagcatc caagcctgag gcatcaaagg taacttcaag atcaagttgt
     9481 gttccaggag caattgttcc ttcagatgga gaaacactta caaatgatcc accaccagaa
     9541 aggtaatgaa cagcattctg gaaaatggtg ggcagatcac cagaccattg cagtggatcg
     9601 ccattaccta aacttggaag catgtttagt gctacaacat tactattaac agcgaggaat
     9661 aactcaccat cattccattc agcaagcgca attgaaccag gagaaagtcc tacgttctga
     9721 acatagccac tataaaagag tgaactaaca ccttgcataa tcggatgccc cggagcaagg
     9781 attgttccta actcaacatc gacaactgcg tctgttgttg atggaacaaa aggtccatat
     9841 ccatcatcga taaatcttcc tgccatttgc cacgcatcgt atgagtaggc aaattgattt
     9901 acaattactt tacctccggc atcaatgtag tcggcaagca aatcaccgat cagggcagga
     9961 tctattccac cagatgcaag ccattgtgta ttgttggatg ttagtacaac atcatattgc
    10021 attaaggtat ccaaagacaa acctggtaat gaacctccgg aaagaatagt ggcatcaatg
    10081 tcatcaaagc tattgagaac aaaggcaaga tcagtcatgt catcatctgg agaaataatt
    10141 aataccttgg atgcgacagc agatttacca actgatttca atttgatttg attggaatat
    10201 tttccgtacg atttagtagt ttgcttttgt gtacccgcta ctgacgatgc agatttgaat
    10261 ggcactgaaa cagtttgcac cgaagctaca cttgcaggtg ctgaagattt taccgcaatc
    10321 gaaaaatcta aattgcttcc tcctgtgtta ccaatggtaa gaatttgcgt tgagcttttc
    10381 cctgtattca gagatgcact taatgaatct ggcgatacta caatctccgg tgcaacgact
    10441 ccagttccct taactgaaac cacaagagat ggattgattc catcattggt taaaataata
    10501 atgttgccgg atgatgtgcc caatgatgaa gcggtgtagg taacttttat agctgcatta
    10561 ccaaacggca caacgaacaa cgagtcagct tcagcagtaa agtctgtatt atcagaagca
    10621 atatctgtaa tcgttaatgg ttcggatcca gcattgtgca catatacaaa ttggcttgca
    10681 aaatctgttt caaacacatt tccaaaatca agtgaatcag gttcgactga tgctattggg
    10741 aagccgttgt ttgtaacgtt caatgtaaga tgaactgatg tgctgtcttg caatggatca
    10801 ttatgagaaa tatcaagagt ttgattgtat gaaccactca acagattatt cgcatcaaat
    10861 gtgatcgtta catcaaggct tgcaccaggt gctactgtac ctgacaaagg tgaagcagtt
    10921 gcaaaaccac taggtacact taaagcaaaa ttcaaaacgc ccaagcctgt attgctaatt
    10981 gttagaattt gcgaaccagt tagtgcagtc tgtaagtctt gtgtcaatga agttgccgac
    11041 aatgttataa caggtgcaaa gtttgtagcc tgttgaagaa tgttggaaac gcttgagaag
    11101 ttaccaaaga aatctgcaga cttaatcgca aagtaataaa ccattcctgg cagcaatcca
    11161 gaaattgtta acgactctgt tgctcctgct ggttgaggtg ctggtgggtt aaggtattgt
    11221 gttgcatcac caaagttttc atctgtaatg ggtgatgttg aataacgaat gtcataagca
    11281 gatgcagaac catttccgga atcaagtggt gaagt
//
LOCUS       DAOOQW010000058        11245 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_7243_length_11245_cov_6.233423, whole genome shotgun sequence.
ACCESSION   DAOOQW010000058 DAOOQW010000000
VERSION     DAOOQW010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 11245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11245
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7243_length_11245_cov_6.233423"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..347
                     /locus_tag="PK281_05160"
     CDS             <1..347
                     /locus_tag="PK281_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HOY48769.1"
                     /translation="QDGAVIHATFEQAATTIGNRVSIGHNALVHGCTLHDNVLIGMGA
                     IVMDNAVVHSGSIIAAGAVVLENSVVESGWIWAGVPAKPVKKVSEELVRGQIDRIADN
                     YLKYASWFKDEE"
     gene            complement(348..1088)
                     /locus_tag="PK281_05165"
     CDS             complement(348..1088)
                     /locus_tag="PK281_05165"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOY48770.1"
                     /translation="MVTEQNPPLHKVRYLLAGFVFFMSFLSLNAQVKVSGTVYDKESG
                     RPLPYAFVIIQRTQNGVFCDQRGQFSLDVQPTDSLLFSLTGYQFTKVLLGDSVRKPEY
                     SLRIPLVVKPVKLRTFEVKAPKTFDQILADLEKAESSKVHHTTSVSNAIESPITFLYM
                     QFSREGKAIRRISELRAEDQKQALIKELFTKYMLAHIIDLDEKDMDDFIAFSGLSNTF
                     NLFDTEYELVVYVKNRYVDYKKYRGIED"
     gene            complement(1096..2637)
                     /locus_tag="PK281_05170"
     CDS             complement(1096..2637)
                     /locus_tag="PK281_05170"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006259844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HOY48771.1"
                     /translation="MQDKIQRLEDMKSEALLGGGAKRIESQHKKGKLTARERIALLLD
                     EGSFEEIGQLVTHRSSNFGLEKQKFYGDGVVTGFGTVHGRTVYVFAQDFTVFGGSLAE
                     AHATKICRIMDLAMQNGAPVIGLNDSGGARIQEGVVSLGGYADIFYRNTLASGVIPQI
                     SAIMGPCAGGAVYSPAITDFILMVEDTSYMFVTGPNVVKTVTHEEVSSEELGGASAHS
                     VKSGVTHFSCTNGVSCIEDIKKLLSYVPSNCEEEAPRLPYTPGNEKRPVLNTIIPENP
                     NQPYDIREVIDQVIDNDTFFEVHKNFAENIVVGFARLGGRSIGIVANQPAYLAGVLDI
                     KSSQKAARFVRFCDCFNIPLLVFEDVPGFLPGTDQEWNGIITNGAKLLYAFCEATVPR
                     VTVITRKAYGGAYDVMNSKHIGADMNYAWPSAEIAVMGAKGAAEIIFKKEIEEAPIPA
                     EKLLEKEREYAEHFANPYSAAERGYVDEVIHPEQTREKLIRAFKMLENKVAKLPRKKH
                     GNIPL"
     assembly_gap    2745..2844
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2845..3568)
                     /locus_tag="PK281_05175"
     CDS             complement(<2845..3568)
                     /locus_tag="PK281_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013069520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOY48772.1"
                     /translation="MSGHSRWANIKHRKGAKDKRRSKLFTRLLKELMMASRMGGPDPD
                     ANPRLRLAIQNAKGANIPKDTITRNVEKGAGIGGETFVEPTYEGYAPSGIAVFVECAT
                     DNLNRTVQDVRMVFNKYGGNLGTNGSLSFVFDRMGVFEIPQGNLDEDEFTMEMIDANA
                     NEIVLEEGMFTVYVPFESFGEMQRKLESMGIEAESAGLQQIPKERTTLDLEAAQKVMK
                     LIEMLEDLDDVQQVYHNLELTEE"
     gene            complement(3617..4261)
                     /gene="can"
                     /locus_tag="PK281_05180"
     CDS             complement(3617..4261)
                     /gene="can"
                     /locus_tag="PK281_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HOY48773.1"
                     /translation="MKPVERLLLENKAWAAEKLETDPAYFLRQSKDQSPEFFWIGCSD
                     SRVPANEVTNTEPGEIFVHRNVANLVVNTDLNLLSVLQYAVDVLKVKHIIVCGHYGCG
                     GVKAAMTQQSFGLINKWLRNIKDVYRFHRVELDGISDHEKRADRLVELNVNEQIHNLA
                     KTSIVQKAWASRQSPVLHGWVYGLDDGILHEIINLDHTYNVDPIYRFEPDELKD"
     gene            complement(4264..5823)
                     /locus_tag="PK281_05185"
     CDS             complement(4264..5823)
                     /locus_tag="PK281_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007847302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HOY48774.1"
                     /translation="MPHQLPLIERIKKDLPASVMVFLVALPLCLGIALASGAPAFSGI
                     LAGIIGGIVIGSFSGSHISVSGPAAGLTVIVVDSIKQLGSFEAFLLAVCISGVLQIVL
                     GIIKAGVMANFFPSSVIKGMLASIGLVLILKQLPKALGYDFDFEGDESFEEAGGHNTF
                     TDIYYAFLNLRPGAVIISISSLLIMLAWDSFVTKLGGFFRFVPGALMAVATGILFNEA
                     FGLWMPELFLEASHRVDLPTGILHSGLSDLILFPDFNAWNNPKIYSVAFTIAVVASIE
                     SLLSIEAGDQIDPYKRITPLNRELVAQGIGNLCSGFIGGLPVTAVIVRTSANVLAGAV
                     SKYSTIIHGILLIISILLFPLLLNSIPLSSLAAILLIVGFKLTKPSLFVQIYKKGLTQ
                     FIPFVITILAILFTNLLIGIGIGLASGLFFVLRSNFRRSISVTIDKENVLIRLRHNVS
                     FLNKTLLREALMNVKNGSTILIDGTHAVFIDQDIIETIETYCSSAEEKNILVHIKRTK
                     TSQNEYFRKND"
     gene            complement(5980..8952)
                     /locus_tag="PK281_05190"
     CDS             complement(5980..8952)
                     /locus_tag="PK281_05190"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOY48775.1"
                     /translation="MRFNFQMQDTQLIFSFYRHPSFGLSLDAYLVSLLENKTFSYNYQ
                     RLVYDRMNDYAYPYTEEEKKVLRKISELSPKSIEQQFNKKQIRSNLFLEKLANDKEQM
                     KMLSAYFDRRLSACLELLKGQKVYWKERVSDHPGMQSFLVESEPAHVTYFFDRQPEGI
                     QYRLMVEHDSKKLDLKKPGAEILSQSPCWLLLGNSIYHFDDGTDGNKITPFLKQQELH
                     IPDKLADKFLESFMLRASRRFHVQYSGFNVLTEPERIQAVISIENSLDKSPVLQLNFQ
                     YDRFTVTAAQEQEMIVSLQREENGMKLMRFTRDKQFELNKTGILESTGLKRVKPSWFL
                     PEHSEQKNWTRDQLLEWLSKHKDVFSQQEIELDIVLEGKRFIAEQARVETRVVKEDTD
                     WFDVLAVVLIGGFEIPFIKFRRNILEHDRYFKLPDGRMMLLPEAWFSEYADLFEFGET
                     NDSSIRLHKQHEGLLNNHPLLNGDLNTRLSEAGKSLYTDSQRTEFVVSENLNATLRDY
                     QKKGFDWMCRLSLKGLGACLADDMGLGKTLQVISVLLWSKERQVPKLESPAPQIQLDL
                     FGFSGEIPRADWKIPSLIVMAPSLVYNWENELRKFAPTLRIHKHLGQRRTTDPQHFGR
                     FDIVLTTYGIVRNDVDLLKQTEFDYIVLDESQLIKNARSVSFQTVKQLVAQQRIVLTG
                     TPVENSLTDLWAQLTFLQPGLLGSFKFFRQEFVIPIEQQQDAAKLEKLRKIIQPFILR
                     RTKEEVAPELPELTRSVHYCEMSPEQKEYYEEQKSVYRNKILENISREGLEKSQLLIL
                     RGLTHLRQIAIHPQLLDSSYTGESAKMDQVLELLSQLRESGRKVLMFSPFVRHLNIYR
                     KYFQEQKLPFSFLAGEVPMQQRAGVIQEFDQHTGHRVFLIQLKTGGSGLNLTKADNVF
                     LLDPWWNPAAEDQAIARSHRIGQHQPVFAWRFITKDTIEEKILHLQERKTRLANDILE
                     GTSSFRTLTEEELTDLFG"
     gene            complement(8995..10182)
                     /locus_tag="PK281_05195"
     CDS             complement(8995..10182)
                     /locus_tag="PK281_05195"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOY48776.1"
                     /translation="MRKLLADRLFPVESESIENGILCIDDQDKVISVLKPGDEGYNPE
                     DAEHYDGWIIPGMVNTHCHLELSHLKGIVSEQTGLDGFVEELMQVRSAPEEIQVAAML
                     DADFDMWNRGIVAVGDISNSARSFPIKEKSNIRYFTFIERFGLNPDFADLAFLTGIEL
                     LKQLQSLSRNTDGNLSPHAPYSVSEQLLSKLVEHIANSGGVLSIHNQETASENEFFKT
                     GGGRMQQRMSNMNISTERFVPSGKSSLETLIPKLPAANRIQLVHNTFSSETDIRIALD
                     KLPHVFWCVCPSANQYIESKLPPIKSLRAIHCEITVGTDSLASNHQLDLLAELRLLQQ
                     ADQSVTSDELIRWSTLNGARLLGFDSELGSFSKGKKPGIVLIEHVDKINGLILPESTS
                     RML"
     gene            10251..>11245
                     /gene="rlmN"
                     /locus_tag="PK281_05200"
     CDS             10251..>11245
                     /gene="rlmN"
                     /locus_tag="PK281_05200"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOY48777.1"
                     /translation="MSETRKDIRTLSLSELEAWFSEQGQQRFRSKQVYEWLWKKSAHS
                     FAEMTNLSKDLRAKLEEHFVIRGIEVASFQKSNDRTIKTAFRLHDGPVVEGVLIPTSS
                     RMTACISSQIGCSLTCKFCATGKLKLLRNLDAAEIYDQVVAIRRQSEENYQIPLSNIV
                     YMGMGEPLLNYKNVLASIEKITSPEGLGMSPQRITVSTAGISKMIKKLGDDEVKFNLA
                     LSLHAANDEKRSKIMPINDTNTLDVLAEALQYFYDKTGTRPTLEYIIFKGFNDSIEDA
                     RELAAFVKRVPSKVNIIEYNPIDEGEFQRATDEQLNIFTTFLEQKGVIVNVRRSRG"
BASE COUNT         3202 a         2388 c         2283 g         3272 t
ORIGIN      
        1 ttcaagatgg tgctgtaatt cacgccacat ttgaacaagc tgctacaaca attggtaatc
       61 gagtttcgat cgggcataat gcgctggttc atggttgcac cctgcacgac aatgttctca
      121 ttggaatggg cgctattgtg atggataatg ctgtagtaca ttcgggtagc atcattgctg
      181 ccggagcagt tgtactcgaa aatagcgtag tagaatcagg ttggatttgg gctggtgtgc
      241 ctgctaaacc tgtgaagaaa gtgagcgaag aacttgtgcg cggacaaata gatcgcatcg
      301 ccgataatta cctcaaatac gcatcctggt ttaaagacga agaataatca atcttcgata
      361 cctcggtatt tcttgtaatc cacataacga ttcttcacat aaaccaccaa ttcgtattcg
      421 gtatcgaata ggttgaatgt attcgacaag cctgaaaaag caatgaagtc gtccatgtct
      481 ttctcatcga gatcaataat gtgagctaac atgtacttag taaagagttc tttgataagc
      541 gcttgctttt gatcttctgc tcgtagttcc gaaatgcgac gaatggcttt tccttcgcgg
      601 ctaaactgca tgtacaagaa cgtaatcgga ctttcgatgg cgttacttac cgaagtggtg
      661 tgatgtactt tggagctttc ggctttctct aaatcggcta aaatctgatc aaaggttttt
      721 ggagctttta cttcgaacgt gcgcagtttt acgggtttta caaccaatgg aattcgtaat
      781 gaatattccg gtttacgaac cgaatcgccc agtaatactt tagtaaactg atatcctgtt
      841 aaggaaaaca agagcgaatc tgttggctga acatctaaag aaaactgtcc gcgttgatca
      901 cagaaaacgc cattttgtgt gcgttgtata atgacaaaag cgtaaggcaa tggtcgaccg
      961 ctttctttgt catacactgt tcccgatact tttacctgtg cattcaggct caagaaggac
     1021 ataaaaaaca caaagcccgc aaggaggtag cgaactttgt gtaaaggagg attttgttcc
     1081 gtaaccacta taaaattata acgggatatt cccgtgtttc ttgcgtggaa gtttcgctac
     1141 tttattttcg agcattttaa acgcacgaat tagtttttcg cgtgtttgtt cggggtgaat
     1201 tacttcgtct acataaccac gttcggcagc gctgtatgga ttggcaaaat gctcagcgta
     1261 ttcgcgttct ttttcgagca atttctcagc aggaattgga gcttcttcga tttctttttt
     1321 gaagataatt tctgctgctc ctttcgcgcc cattactgca atctcggcac ttggccaagc
     1381 ataattcata tcagctccaa tatgcttaga attcatcaca tcgtatgctc caccgtatgc
     1441 tttgcgtgta ataacggtaa cacgcggaac ggttgcttca caaaatgcat aaagcaattt
     1501 tgctccattg gtaatgatac cattccactc ttgatcggtt cccggaagga atcccggaac
     1561 atcttcaaac actaacaaag gaatattgaa acaatcacag aaacgtacga aacgagccgc
     1621 tttttgtgaa gattttatat caagcactcc tgccaaataa gctggttggt tagctacaat
     1681 tccaatgctt cgtccgccta aacgggcaaa tcctactacg atattttcag caaagttctt
     1741 atgtacttca aagaaggtat cgttatctat aacttgatca atcacctcgc ggatatcgta
     1801 aggttgattc ggattttcgg ggatgatggt gtttaatacc gggcgtttct cattcccagg
     1861 tgtgtaaggt aatcgaggcg cttcttcttc gcagttcgag ggaacgtagc tcaacagttt
     1921 cttaatatcc tcaatgcacg aaacgccatt tgtacacgag aaatgcgtta ctcccgattt
     1981 tacactgtgt gccgatgcac cacctaattc ttcggagcta acttcttcat gtgtaacggt
     2041 ttttaccacg ttcggacccg tcacaaacat gtaggaagtg tcttccacca tgagaatgaa
     2101 atcggtgatg gcaggagagt aaacagctcc accagcacat ggtcccatga tagccgaaat
     2161 ttgaggaatc acgcccgatg caagtgtgtt acggtaaaag atatctgcat atccacctag
     2221 cgaaacaaca ccttcctgaa tacgtgctcc acccgaatcg ttcaagccaa taacaggtgc
     2281 gccattttgc atcgctaaat ccatgatgcg gcaaatttta gtggcatgtg cttcggctaa
     2341 agatcccccg aaaacagtga aatcttgagc aaatacatat accgttctgc catgaacagt
     2401 tccaaaacca gtcactacac catcgccgta aaacttctgt ttttccaaac cgaaattgct
     2461 gcttcggtga gtaacaagtt gtccaatttc ttcaaaagaa ccttcatcta acaataaagc
     2521 aatgcgttcg cgggcagtga gtttgccttt tttatgttgt gattcgatgc gctttgctcc
     2581 tccacccaaa agggcttccg acttcatgtc ttcgagacgc tgaattttat cctgcatgct
     2641 gttagtggtt ttatcggtaa tcggattcat ggctaaagtt cgttggcgag ctcttcggtt
     2701 agctcaaggt tgtgatatac ttgctgaaca tcgtccaaat cttcnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnngctctt cggttagctc aaggttgtga tatacttgct
     2881 gaacatcgtc caaatcttca agcatctcaa taagcttcat cactttttgg gctgcttcta
     2941 aatcgagtgt tgtgcgttct ttaggaattt gttgtaatcc agcactttcg gcttcaatgc
     3001 ccatgctttc gagcttgcgt tgcatttctc caaacgactc gaatggtaca taaacggtaa
     3061 acatgccttc ctccaaaacg atttcattcg cattcgcatc aatcatttcc atggtaaatt
     3121 cgtcttcatc gaggttacct tggggaatct caaatactcc cattcgatcg aacacaaaac
     3181 tcaaagaacc attcgtacct aagtttccgc catatttatt aaacaccatg cgcacatctt
     3241 gaacggtacg attcaaattg tcggttgcgc attcaacaaa caccgcaatg cccgatggtg
     3301 cgtagccttc gtatgttggt tctacaaagg tttccccacc aataccagct cctttttcta
     3361 cattacgtgt gattgtgtct ttaggaatgt tagctccttt ggcattttgg atggctaaac
     3421 gtaaacgtgg atttgcatct ggatcagggc cgcccatgcg agaagccatc atcaattcct
     3481 ttaaaaggcg cgtaaaaagc ttactacgac gcttgtcttt agctcctttg cggtgtttga
     3541 tgtttgccca tctgctatgt cctgacatga atgctgcttt ttggaggccg ctaagttaac
     3601 aaagtgcggc gctcaatcag tctttcaatt catcgggttc gaagcgataa atagggtcta
     3661 cattgtatgt atggtcaaga ttaataatct catgtagaat accatcatcc agtccataaa
     3721 cccatccatg taaaaccgga ctttgacgtg atgcccaagc tttttgaaca atggaggttt
     3781 ttgccagatt gtgtatttgt tcgttcacat ttaactcaac caagcgatct gctcgttttt
     3841 catgatcact aattccatcc aattcaacac ggtggaaacg gtaaacatct ttaatgttac
     3901 gcaaccattt attgatgagc ccaaatgatt gttgagtcat cgcagccttc acaccaccac
     3961 agccataatg tccacaaaca ataatatgtt tcactttcaa cacatctact gcatattgca
     4021 aaacgcttag aaggttgagg tcggtattaa ccactaagtt agcgacgttt cggtggacaa
     4081 aaatctcacc cggttcggta ttagtgactt cgttagctgg tacgcgactg tcggaacaac
     4141 caatccaaaa aaactctggg gattggtctt tcgattgacg aagaaaatag gctgggtcag
     4201 tttctaattt ttcggcagcc caagctttgt tttcgagtaa taatcgttca acgggtttca
     4261 tgctcagtcg tttttccgga aatattcatt ttgacttgtt tttgtgcgct tgatatggac
     4321 aagtatattc ttctcttcag cagatgagca gtacgtttcg atggtttcta taatatcttg
     4381 atcaataaaa accgcatgcg taccgtcaat tagaatagtg ctaccattct tcacattcat
     4441 caaagcttct cttagcaatg ttttattgag aaatgaaacg ttatggcgaa gccggattag
     4501 gacattttct ttatcgatgg ttacacttat cgaacgtcta aaattggaac gaagcacaaa
     4561 gaaaagtccg ctcgctaaac caattccaat tccgataagc agattggtaa atagaatggc
     4621 taaaatggtg atgacaaatg gaatgaactg ggtaagtcct ttcttgtata tctggacaaa
     4681 taagcttggt ttggtaagtt tgaaaccaac aattagcaaa atagcagcaa gcgaagaaag
     4741 tggaattgaa tttagaagta aggggaaaag caggatgcta ataatcagta atattccatg
     4801 aattattgtt gaatatttgg aaacggctcc cgctaaaaca ttggctgaag tacgaacaat
     4861 aacagctgtt acaggcaatc caccaataaa tccggaacat aaattcccaa tgccttgtgc
     4921 aactaattcg cgatttagag gcgtaatgcg tttataagga tctatttgat ctccagcttc
     4981 aatactgagc aagctttcaa tactggctac aaccgctatt gtaaaggcaa ctgaataaat
     5041 tttcggattg ttccaagcat taaaatcagg gaaaaggatt aaatcggata gtccagaatg
     5101 taaaatgcct gttggtaaat ctacacgatg acttgcttct aagaatagtt caggcatcca
     5161 cagtccaaat gcttcattga aaagaatccc cgttgctact gccattaagg ctcctggtac
     5221 aaatcgaaag aatccaccta gtttcgttac gaacgaatcc caagcaagca taatgagtaa
     5281 tgaacttatg ctgataatga ctgcgcccgg tcttagattt aaaaaagcat agtagatatc
     5341 agtaaatgta ttatgtccgc cagcttcttc gaaactttca tctccttcga aatcgaaatc
     5401 atatccaaga gccttgggta attgcttaag gataagcaca agtccaatgg atgccaacat
     5461 tcctttaata accgacgagg gaaagaaatt ggccattaca ccggctttaa taatacctaa
     5521 cacaatttgc aaaacacctg aaatacaaac ggctagcaaa aatgcttcga acgaaccgag
     5581 ttgtttgatt gaatcaacaa caattactgt taatccagca gcaggtccac ttacgcttat
     5641 gtgtgaacca ctgaaagaac caataacaat cccacctata atacctgcta aaattcccga
     5701 aaaagcaggt gctccgctag ccaacgctat tcccaaacat aagggcagcg ctactagaaa
     5761 taccatgacg gatgcaggaa gatctttttt aattctctct attaatggta actgatgagg
     5821 cattgacggg ttgggctttt ttgaaaggcg ggtatattcc ttttaatcac tcagatatta
     5881 attctgttta agcagatttt aatctgaaag cattttgcta aactaaggca taatcagatt
     5941 tgcctaacag cacattccag tttttgtgga atatttcaac tagccaaaca ggtcggttaa
     6001 ctcttcctcg gttaatgttc taaatgagga agttccttct agaatatcgt tggcaagtcg
     6061 ggttttacgc tcctgtaaat gcaggatttt ctcttcaatc gtgtctttgg tgatgaaacg
     6121 ccaagcaaat acgggttgat gttgtccgat acgatgcgag cgagcaatag cttgatcttc
     6181 ggctgccgga ttccaccacg ggtcgagtag gaaaacgtta tcggctttgg tcagattcaa
     6241 accagaacca cccgttttca attgtatcaa gaaaacccga tgtccagtat gttgatcaaa
     6301 ttcttgaatc acaccagcgc gttgctgcat gggaacttct ccagctaaaa acgaaaaagg
     6361 cagcttttgc tcttggaaat acttccgata aatattcaaa tgccgcacaa agggtgagaa
     6421 catgagcacc tttcgaccac tttcgcggag ctgagaaagc aattccagca cctgatccat
     6481 ttttgctgat tcgcccgtat agcttgaatc taacaattga ggatgtatcg caatttgacg
     6541 caaatgtgtt aaaccacgaa gaatcaacag ctgacttttc tccaaacctt cgcgggaaat
     6601 gttttcgagg attttgttcc gataaaccga tttctgttcc tcgtaatatt ccttttgctc
     6661 tggcgacatt tcacaataat gtaccgaacg tgttaattca ggaagttcgg gtgcgacttc
     6721 ttctttggtt cgtcgcagaa taaacggctg aatgattttt cttagctttt caagtttagc
     6781 tgcatcctgc tgttgttcaa taggaatcac aaattcttgg cggaagaact taaaacttcc
     6841 caataaaccg ggttgtagaa aggtgagttg agcccatagg tctgtaagtg aattctctac
     6901 aggcgtgccc gttagcacga tacgttgttg cgctactaat tgcttcaccg tttgaaaact
     6961 aaccgaacgc gcatttttaa tcaactggct ctcatccaac acgatatagt cgaactcggt
     7021 ttgtttcaaa agatctacat cgtttcggac aattccataa gtagtgagga caatatcgaa
     7081 tcgaccaaaa tgttgaggat cggtggtgcg acgttgacca aggtgtttat ggattcgaag
     7141 tgttggggca aattttctaa gctcattttc ccaattgtaa actaacgaag gagccatcac
     7201 aatgagtgaa ggtattttcc aatcagcacg cggaatttca cccgaaaaac caaacaaatc
     7261 gagttgtatt tgtggggctg gagattctaa tttgggcact tgtcgctctt ttgaccacag
     7321 caaaacggat attacctgaa gagtttttcc caatcccata tcatccgcca aacaagctcc
     7381 taagcctttc aacgaaagac ggcacatcca atcgaagcct tttttctggt agtcgcgtaa
     7441 ggtggcattc aaattctcgc tcaccacaaa ttcggtacgt tgcgaatcgg tataaagcga
     7501 ttttcctgct tcacttaatc gagtattcaa atctccattt agcagcggat ggttattcaa
     7561 taaaccttcg tgttgcttat gaagccgtat gcttgaatcg tttgtttcgc caaactcaaa
     7621 taaatcggca tactccgaaa accatgcttc tggcaatagc atcatccgcc catcgggaag
     7681 cttaaaatag cggtcgtgtt ccagaatatt cctgcgaaac ttgataaacg ggatttcaaa
     7741 tccgcctatt aacacaactg ccaacacatc aaaccaatcg gtatcttctt tcactactcg
     7801 ggtttcgaca cgtgcctgtt cggcgataaa gcgtttccct tcgagcacaa tatccaactc
     7861 gatttcttgt tgcgaaaaaa catccttgtg cttcgaaagc cattctaaca actgatcgcg
     7921 cgtccaattt ttctgctccg aatgttcagg taagaaccag gaaggcttaa cccgtttcag
     7981 tccagtgctt tctaatattc cggttttgtt gagttcaaat tgcttgtcgc gcgtaaatcg
     8041 catgagcttc atcccatttt cctcccgttg caacgaaaca atcatttctt gctcctgtgc
     8101 tgcagtcaca gtgaagcgat cgtactggaa attgagttgt aaaaccggac ttttatctaa
     8161 gctattctca atgctgataa ctgcttgaat acgttcgggt tcggttaaaa cattgaatcc
     8221 tgaatactgt acatgaaatc tccgagaagc tcgaagcata aaggattcga ggaatttatc
     8281 agctaattta tctggaatgt gaagttcctg ctgtttgagg aacggtgtga ttttattccc
     8341 atccgttcca tcatcaaaat gataaatcga attgccaagt aataaccaac aagggctttg
     8401 acttagaatt tcggcaccgg gtttctttaa atctaacttc ttggaatcat gctcaaccat
     8461 cagtcgatat tgaattcctt caggttgacg atcaaaaaaa tacgtaacgt gagccggctc
     8521 cgattctacc aggaaactct gcatgcccgg atgatccgaa acacgttctt tccaatatac
     8581 tttttgtcct ttgagtaatt cgagacaagc cgataatcga cggtcgaaat aggcgctgag
     8641 cattttcatc tgctctttat cattcgccag tttctcgaga aacaaattgc tgcggatttg
     8701 ctttttgttg aattgctgct cgatcgattt cggacttagt tcgctgattt tacgcaatac
     8761 tttcttttcc tcttccgtgt atggataggc gtagtcgttc attcgatcgt aaaccagtcg
     8821 ctgatagtta taggaaaacg ttttattttc taataaacta accaaataag cgtctagact
     8881 taagccaaac gaaggatgac gataaaagct gaaaatcagt tgagtatcct gcatttgaaa
     8941 attgaaacgc attcttagaa agcagaatac ggaacaatcc acaattttaa cgaatcaaag
     9001 cattcgggaa gtgctttcgg gtagaattaa cccatttatt ttatcaacat gctctattag
     9061 cacgattcca ggtttcttgc ctttgctaaa actgcctaat tcagaatcaa atccgagtaa
     9121 tcgagcccca tttaaagtac tccaacgaat gagttcatca ctagtaacac tttggtcggc
     9181 ttgttggagt aaacgcaatt cagctaatag atcgagttga tggttagagg ctagactatc
     9241 ggttcctacc gtgatttcac agtgaatagc acgaagtgat tttataggcg gaagtttaga
     9301 ttcgatgtat tgattggctg atggacacac acaccaaaaa acgtgaggaa gtttatctaa
     9361 ggcaattcga atatctgttt cgctgctgaa agtattgtga accagctgaa tgcgattggc
     9421 agccggcaat ttgggaatta aagtttctag cgaagatttt ccagatggca caaagcgctc
     9481 agtcgagata ttcatgttac tcatgcgttg ttgcatgcga ccgccacctg ttttgaaaaa
     9541 ctcgttttca ctggctgttt cttgattatg aatgctcaaa acgccacccg aatttgctat
     9601 gtgttcaacc aatttggaaa gtagctgctc tgaaaccgaa tatggcgcat gcgggctcaa
     9661 attcccatcc gtatttcgac ttaaagattg cagttgtttg agcaattcta ttccggttaa
     9721 gaatgctaag tcggcaaaat ctggattcaa tccgaaacgc tcaataaacg tgaaataacg
     9781 gatgttcgat ttctccttaa ttggaaagct acgagcacta ttggaaatgt cgccaaccgc
     9841 cacaattcct ctattccaca tgtcgaaatc ggcatcgagc attgctgcaa cttggatttc
     9901 ttcaggtgcc gatcgtacct gcataagttc ttcaacaaat ccatccaaac ctgtttgttc
     9961 acttacgatt cctttcaaat gtgaaagttc caaatggcaa tgtgtattca ccattccggg
    10021 aattatccat ccatcgtaat gttctgcatc ttcggggttg tagccctcat cacccggttt
    10081 tagcacagat attaccttat cctgatcatc gatgcaaaga attccattct cgatagattc
    10141 agattcaaca ggaaagagtc ggtcggctaa aagcttgcgc atgcgacaaa ggtaatcggc
    10201 taaacgtaat ccaatccaag catctgaata gcttattgta tctttgacgc atgtcagaaa
    10261 cgcgtaagga cattcgtaca ctcagtttaa gcgaactgga agcctggttt tcggagcagg
    10321 gacagcagcg ttttcgctct aaacaggtat atgaatggct ttggaaaaag tcggcacatt
    10381 cgtttgctga gatgaccaat ttgagcaagg atttacgcgc aaaattggaa gaacattttg
    10441 tgattcgagg tattgaagtt gctagttttc aaaagagtaa cgaccgaacg atcaaaacag
    10501 cgtttcgatt gcatgatgga ccagtagttg aaggcgtgtt gattcctacc tcgagtagaa
    10561 tgacagcttg catctcatcg caaattggtt gcagcttaac ctgtaaattt tgtgcaacgg
    10621 gtaaattgaa acttcttcga aacttggatg ctgcagagat ttatgatcag gttgttgcca
    10681 tccgccgtca aagcgaagaa aactatcaaa ttcccctttc gaatatcgtg tacatgggca
    10741 tgggcgaacc tttgctgaat tataaaaatg tattagcgag cattgagaaa atcacttctc
    10801 ccgaaggttt gggcatgtcg ccacagcgaa ttaccgtatc aaccgctgga atttctaaga
    10861 tgattaagaa attgggtgat gatgaggtga aattcaattt ggcattatcg ctccatgcgg
    10921 ctaacgatga gaagcgtagc aagattatgc ccatcaacga taccaatacc ttggatgtgt
    10981 tggctgaagc acttcagtat ttttatgata aaacgggcac tcggccaacg ctggagtata
    11041 ttattttcaa aggattcaac gattcaattg aagatgcacg cgagttagca gcatttgtaa
    11101 aacgtgttcc atctaaagtg aatatcatcg aatataatcc aatcgacgaa ggtgaatttc
    11161 aacgggcaac ggatgagcaa ttgaacattt tcactacgtt tttggagcaa aaaggagtca
    11221 ttgtgaatgt gcggagaagt agggg
//
LOCUS       DAOOQW010000059        11218 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_7263_length_11218_cov_5.868225, whole genome shotgun sequence.
ACCESSION   DAOOQW010000059 DAOOQW010000000
VERSION     DAOOQW010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 11218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11218
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7263_length_11218_cov_5.868225"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="PK281_05205"
     CDS             complement(<1..662)
                     /locus_tag="PK281_05205"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF018696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOY48778.1"
                     /translation="MALYFHGNAPLKFQHQYLVTRDYIIPYLEKNGGWKENMQVLEIG
                     CGEGGVLKAFVEKGCICTGMDLSTSKIENGKNLQAEEIAAGKLQLFSADIYDQSTMDA
                     FRGKFDLIILKDTIEHIPDQEKVMAQLPVYLKEGGKLFFAFPPWRMPFGGHQQTATTK
                     KGQLPWMHLLPRSLYESYLRKIGEPQPTITSLLEIYDTRISINRFEYILKKTGYTIHH
                     RRL"
     gene            complement(749..4435)
                     /locus_tag="PK281_05210"
     CDS             complement(749..4435)
                     /locus_tag="PK281_05210"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOY48779.1"
                     /translation="MKILTNRSPWLLLVLAILCLLAAEAGFHFTHFPAPTEQKSLLEQ
                     YIKQRATDLDKESEAFRHSGCRDLSFYSNFPAHWDELSLQKNMAFIGYSERSVYFWTD
                     NSVPISSAGLPSIDEKSLLKLKNGWYLVRRYADGPCNLLGLALVCREYSYQNDYLQNS
                     YPGLENLPEGSRIVAVQEDGALPILGDQGQTMAWFLPDAGGADVEKYPTWPVALSIAG
                     LLIFLLAGHQIATSFAKQGKIAGAWAWWLILIGLRTLSFFFHFPGPLVHLALFSPSEY
                     AASNWLPTLGDLLINTVLFLYSSWLLFQLGKSISARPALRKPLIVLLLSVVLFLSWFV
                     GDVIRGLIINSSISLDISDVLSLTTSSYLSFLIIGLLFASLFLLMDRLSRWIAPWLTH
                     AWDLPVLLILTSVVLTIGLYLFDPVDLIVIPVSALILLLIYYSRNQSNGFGYARIIGL
                     IMSFAFLGTYLLQANSSEREHEKRKLLSGKISAERDPIAESLFLEVEKKILTDSILKT
                     YLRPSSVATGQVRDLAQLYFNSYWEKYSINVNVFGADECPMTALYTTSVSDPMKFDWL
                     IDSIGIPTMSDRFFFMDNGSGRISYMARLPILNENRDSIPLGTLYIDFQSRYTPEEIG
                     YPELLLDKMVSTRTDLSNYSYARYNDGKLVSHYGTYPYELSDTLFRKASVEEYTFFQT
                     DKFSHLIYHPLDHAKLHSYVILSLPKAGILGLLTPFSYLVLFFSLLFLIPFLGNALLE
                     GRNLIRISFKRRIQLSIVLILFISLILIGGGTIVYIISNSNQKNLRNISEKIHSLLVE
                     TEYILGKDDGLSPFKSEDIAYALTRQANVFFADINIFDPYGQLYASSRTKIFEEGLVS
                     KKMDPEAFYNLSVKKSSEFIHEENIGKLNYFSAYVPLRNNENRVIGYLNLPYFARQNE
                     LRKEIATFVVAIVNIYVLLIVLVVIAAIFLSNTVTEPLRLIQEKLGNIQLGRKNEMIE
                     WKGHDEIAELINEYNRMVSELAQSADKLARSERESAWREMAKQVAHEIKNPLTPMKLS
                     TQMLKRAWDDGAPGFDQRIDRYTQNLIEQIDSLSHIATEFSNFAKMPKMVKERVDLHG
                     LLQGAIDFHQGEQGVVISMELQCKTPCYILTDKEQMLRVFNNLLRNAIQAIPEDQQGK
                     IDVSLKQENDRYCFAVKDNGTGISDDLRDKIFSPNFTTKNAGMGLGLAMVKNIVETSE
                     GRIWFESALGEGTTFFVELPMDKS"
     gene            complement(4592..5356)
                     /locus_tag="PK281_05215"
     CDS             complement(4592..5356)
                     /locus_tag="PK281_05215"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOY48780.1"
                     /translation="MKRLLQIEWLKLASYRTFYVFTGFYVLLIFLVYYGFDKFTTTPM
                     INLSVVYQFPNVWYYIAYVSSWFAPVLGLLMINLVSNEFSFRTLRQHVIDGLSREEIL
                     KSKLLLAAVISIGAGLVALMSGLLFGFTKGGGANSGDIFSEMTYLLRAMWVCFGLMSA
                     AILISLIVRRSALSILVFISLYWIVEPILGRAWLSDLYPYFPLNSLDEFISSPFSFDS
                     VSFGKQSTPTGVNIAALIYPILFIAASWRLIKTKDI"
     gene            complement(5353..6240)
                     /locus_tag="PK281_05220"
     CDS             complement(5353..6240)
                     /locus_tag="PK281_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013619678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOY48781.1"
                     /translation="MTLLEVDSLLKRYGKNTAVNGVSFRVEKGQVFGLLGPNGSGKTT
                     LLGCLTGILIADQGSFSWFGNEEFSSARKRIGALIETPNFYPYLSAWKNLEIVAKIKG
                     CELSRIDVVLKEVELFDRKNDAFKTFSLGMKQRLAIAGALLCDPEILVLDEPTNGLDP
                     QGINEIRQLILRQKANGKTIVLASHLLNEVEKVCTDVVILKKGNVLSSGKVEQLLTEN
                     KRIELGSNDLSALRNAIQSFTGISNIQDRNGFLIADLADDLDAASINKQLQEKGILLN
                     HLVVVKSDLESEFLKLVQK"
     gene            complement(6241..8226)
                     /locus_tag="PK281_05225"
     CDS             complement(6241..8226)
                     /locus_tag="PK281_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase E1 component subunit alpha/beta"
                     /protein_id="HOY48782.1"
                     /translation="MQLSFKRNGKSDELLLHIYKELVYPRMIEEKMLNLLRQGKISKW
                     FSGIGQEAISVGTVVAMDKEEYILPMHRNLAIFTGRGMPFHQLFTQWQGKFNGYSKGR
                     ERSFHFGSKEHKVVGMISHLGPQLGLADGIALAHKLRKENNVTAVFTGDGGASQGDFH
                     ESVNVAAVWDLPVIFIIENNGYGLSTPSNEQFRFNSFTEKGPGYGIPARQVDGNNVLD
                     VYDAVQEIRADIQKNPRPWLLECMTFRMRGHEEASGTKYVPQELFELWGKKDPVVNYE
                     AWLLQEGVLTDESLASIRAEFKKTIEEGVAITFAEPEITPDTAYEISDVYAPFVSTTQ
                     SPASESKTDKRFIDAISDGLALAMEKHPNLVLMGQDIAEYGGVFKITEGFVERFGKDR
                     VRNTPLCEAAIVGSSLGLSINGMKAMMEMQFADFVTEGFNQIVNNLAKSHYRWGQHAD
                     VVVRMPTGAGVAAGPFHSQSNEAWFTHTPGLKVVYPAFPNDAKGLLLASFEDPNPVMF
                     FEHKALYRSISEPVNDDYFTIEIGKANLLREGKDVSIITYGFGVHWALDVLNKHPEIN
                     ADLLDLRTLLPFDEEAILASVKKTGRVIILHEDCLTGGIGGEIAARISEKCFEYLDAP
                     VVRSASLDTPVPFSSVLEQNFLPKGRFEQQLLDLISY"
     gene            complement(8456..8689)
                     /locus_tag="PK281_05230"
     CDS             complement(8456..8689)
                     /locus_tag="PK281_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48783.1"
                     /translation="MKKVTFAILTVGVALFASCGGNNAEAEKAKADSIAAAAKADSIA
                     KAEADAAAAAAAADTTAKVDTAAAAAAPAPAAH"
     gene            8933..9433
                     /locus_tag="PK281_05235"
     CDS             8933..9433
                     /locus_tag="PK281_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48784.1"
                     /translation="MKKFQMNLAKNLRLIGLTILAGTMTLTYSGCKKEGCTDTDANNY
                     NEEADEDDGTCTYDSEKFIGVYTSTSPCVAGGSWNTTISASSSSKKKVVVTNIGGLGS
                     SATATADIDGSTIKINSQSVIDSDGDSWTVSSTSGTLSGTSINLTITYTFGVSNLTCS
                     ETWNKQ"
     gene            complement(9706..10128)
                     /locus_tag="PK281_05240"
     CDS             complement(9706..10128)
                     /locus_tag="PK281_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48785.1"
                     /translation="MKKMLFIFSAVALATLTACGPSAEELAKKEQATKDSIAAVEKAR
                     QDSIDSAMKEQARLDSIAAAEAAAAEAATAAPVKGSKPTKGTTPKQEEPKKEDPKNMK
                     DAVKGGSNTPTNMKDAVKDQKGGSTTSPKSMKDAMKGK"
     gene            10314..10820
                     /locus_tag="PK281_05245"
     CDS             10314..10820
                     /locus_tag="PK281_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48786.1"
                     /translation="MKHPFFPTSILIILVYVALALPFSSCEPDPGLPSTDSIITVDTL
                     PYNYIYKSYVINNWMCNVSGYHYTPYQLEIKQDTINFNQVLVSNFANISGQVVGVLSS
                     DNSADSIIITVPTQYFSYPSSSNPNIYDTLFVNGNLFVYPDSIIIQQHTKINSFYGDV
                     CIGFGNPQ"
     gene            10890..>11218
                     /locus_tag="PK281_05250"
     CDS             10890..>11218
                     /locus_tag="PK281_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48787.1"
                     /translation="MKSFSLIFLSLCLALSSCDRDGCTDPNANNYDDKYENDDGSCRY
                     DSDPFDGRWEAHDSILAFGVYTADEVRTLDIRILTANRAKVKFFWRNADGTYSDTLEA
                     NSKPNTLS"
BASE COUNT         3176 a         2326 c         2392 g         3324 t
ORIGIN      
        1 agtcggcggt gatggatggt gtagccggtc ttttttagga tgtactcaaa gcggttgata
       61 gaaatacggg tgtcgtaaat ttctagcaag gatgtgatgg ttggttgagg ttcgccgatt
      121 ttacgtagat aggattcgta aagagagcgg ggcagaagat gcatccaagg cagttgtcct
      181 tttttggtgg tagctgtttg ttgatgtccg ccaaaaggca tgcgccaagg tggaaacgcg
      241 aaaaacaatt taccgccttc tttcaggtaa acgggaagtt gcgccattac tttttcctga
      301 tcgggaatgt gctcgatggt atcttttagg atgataagat cgaactttcc acgaaaggca
      361 tccatggttg attggtcgta gatatcggcg gagaaaagtt gaagtttacc agcggcaatt
      421 tcttcggctt gcaggttttt tccattctcg attttagagg tagaaagatc cattcccgtg
      481 cagatgcaac ccttttcgac aaatgctttg agcacaccac cttcgccaca gccaatctcg
      541 agcacctgca tgttttcttt ccaaccacca tttttctcta ggtagggaat gatgtagtcg
      601 cgggtaacta agtactgatg ctgaaactta agcggagcgt tgccgtggaa gtagagagcc
      661 atatcgaaaa tcgttgccga agataggacg aaatcgcaag ggatgaatca tccttgtttt
      721 gagggatggt ttggtagtag tctacagttt aagatttatc catcggtaat tcaacaaaga
      781 aggtggttcc ttcacccaat gctgattcaa accaaatgcg tccttctgat gtttcgacga
      841 tgtttttaac catggccaat ccgagtccca tgccagcatt ttttgtcgtg aagttgggag
      901 agaaaatctt gtctcttaaa tcgtccgaaa tgcctgtgcc gttgtccttc accgcaaagc
      961 aatagcggtc gttttcttgt ttcaggctta catcaatttt tccttgttga tcttcgggaa
     1021 tagcttgaat cgcattgcgc aacaagttgt tgaatacacg caacatttgc tctttatcgg
     1081 tgaggatgta acatggagtt ttgcactgta attccatcga aattactaca ccttgctcac
     1141 cttggtggaa atcgattgct ccttgcaaca agccatgtaa atcgacccgt tccttaacca
     1201 ttttaggcat cttggcgaag ttcgaaaact cggtagcaat atgacttaac gaatcaatct
     1261 gctcaatgag gttttgcgtg taacggtcga tacgttgatc aaatcccgga gcgccatcat
     1321 cccaagctcg tttgagcatt tgggtgctca atttcatcgg tgtaagtgga tttttaatct
     1381 cgtgtgccac ctgtttcgcc atctctcgcc atgcactttc acgttccgat cgcgcaagct
     1441 tatccgcact ctgcgctaac tcgctcacca ttcgattgta ttcgttaatc agctccgcta
     1501 tttcatcatg gcctttccat tcgatcatct cgttctttcg ccccagctgg atatttccaa
     1561 gtttttcctg aatcaaacgc aatggttcgg ttaccgtatt ggataagaaa atggcagcaa
     1621 ttacgaccaa tacaatcagt aaaacgtaaa tattgacaat cgctacaacg aaggtggcaa
     1681 tctctttacg cagctcattt tgtcgggcga aatagggcag gttgaggtaa ccaatcaccc
     1741 ggttttcatt atttcggagc ggtacgtaag ccgagaaata gttgagtttt ccgatatttt
     1801 cctcatggat aaactcactc gatttcttca ccgaaagatt gtaaaaagcc tccggatcca
     1861 tctttttcga aactaagcct tcctcaaaaa tcttcgtgcg tgatgaagca taaagttgtc
     1921 cgtaaggatc gaaaatattg atatcagcga agaatacgtt tgcctgtcgg gtgagcgcat
     1981 acgcaatatc ctctgattta aacggactca atccatcgtc tttcccaagt atgtattcgg
     2041 tttcaactag taaagaatgg attttctcac tgatgttgcg caggtttttc tgattgctat
     2101 tggagatgat gtaaacgatt gttcctcctc caattaatat cagggaaatg aacaaaatca
     2161 gcacgatcga caactgaata cgacgcttaa agctgatgcg gatgaggttt cttccttcga
     2221 gtagcgcatt ccccaaaaat ggaatcagaa acaacaaact gaagaataat accaaatagg
     2281 aaaatggtgt gagaagcccg agaataccgg ctttgggcag acttaaaatt acgtaagaat
     2341 gcagttttgc atgatcgagt ggatgataaa tcaggtggct gaatttatcg gtttggaaga
     2401 aggtgtattc ctcaacagaa gctttccgga aaagtgtatc cgacaattca tatggatagg
     2461 ttccataatg tgaaaccaat ttcccatcgt tgtatcgcgc gtagctgtag ttactcagat
     2521 cggtgcgggt actcaccatt ttatcgagta gcaactcagg atatccaatt tcttcgggtg
     2581 tgtaacgcga ttggaagtcg atgtacaacg ttcctaatgg aattgaatct cgattctcgt
     2641 tgaggatggg caatcgagcc atgtagctga ttcgtccaga tccattatcc ataaagaaga
     2701 accgatcact catagttgga attccgatag aatcgatcaa ccagtcgaac ttcattggat
     2761 cgctcaccga agtggtgtac aaagctgtca tcggacattc atccgccccg aaaacattca
     2821 cattgatgct atatttttcc caatagctgt tgaagtacaa ttgagcaagg tcgcgtactt
     2881 gtccggttgc tacgctgctc ggtctcaaat aggttttgag gatagaatcg gtgaggattt
     2941 tcttttctac ttcgaggaat aagctttcgg cgatagggtc gcgttcggcg gagattttac
     3001 cggagagtag tttacgtttc tcatgttcgc gttcggagct attggcttgt aacagataag
     3061 ttcccagaaa tgcgaaactc atgatgagtc cgataatccg cgcataccca aaaccgttac
     3121 tttgattacg ggagtagtag attagaagta aaatcaaggc tgaaaccgga atcacaatca
     3181 gatcgactgg atcaaacaga tacaatccaa tggttaaaac gacgctggtc agtatcaata
     3241 aaaccggtaa atcccatgca tgtgttaacc aaggtgcaat ccagcggcta agtcggtcca
     3301 tgagcaaaaa cagggacgcg aaaagcaaac cgataatgag gaacgatagg taactgctgg
     3361 tggtcagaga aaggacatca gaaatatcga gcgagatgct ggagttgatg atcaaacctc
     3421 gaatgacgtc cccaacaaac cacgaaagga atagcactac gctcagaagc agcactatta
     3481 atggttttcg tagagccggt cttgccgaaa tgctttttcc taactgaaat aaaagccagg
     3541 aagagtagag gaaaagaacg gtgttgataa gcaagtcgcc caaagttggc aaccaattac
     3601 tagccgcgta ttcacttgga ctaaacagtg ccaaatgcac gagtggaccg ggaaaatgga
     3661 agaagaagct taatgttctt aaaccaatca atatcaacca ccaagcccaa gctccagcaa
     3721 tttttccttg tttggcgaat gaagtagcga tttgatgtcc tgccaagagg aaaatcaata
     3781 aaccagcaat ggagagagct accggccaag taggatattt ttctacatct gcaccacccg
     3841 catcgggtag aaaccaagcc atcgtttgac cttggtcacc tagaattggc aatgcaccat
     3901 cttcctgaac agctactatt cgtgagccct ccggaagatt ttctaaaccc gggtaactat
     3961 tttgcaagta atcgttttgg taggagtatt cccgacaaac gagcgctagt ccgagcaaat
     4021 tgcaaggccc gtcggcgtat cgtcttacga gataccaacc atttttgagt ttgagaaggc
     4081 ttttttcatc aattgagggt aaaccagcgg aggaaatcgg aacggaattg tcggtccaga
     4141 aatacaccga acgctcggaa tagccaataa aggccatgtt tttttgaagc gagagttcat
     4201 cccagtgagc ggggaaattg gagtagaatg aaaggtctct acaacccgaa tgacgaaagg
     4261 cttccgattc tttatcgaga tcggtagcgc gttgtttaat gtattgttcg agcagcgatt
     4321 tctgttctgt gggagcagga aaatgggtaa agtgaaatcc cgcctcagcc gccaacaagc
     4381 agagaattgc caataccagc aataaccaag gactacgatt cgtcaggatt ttcacagaaa
     4441 ccaaaaatac gtaatcagaa actggtatag agtattaagt attatgtatt gagatggatt
     4501 ggagaaatta agtcgaggct cacttaaaat gtcttccctc tttctcatta cttcatattc
     4561 tcactttgta ctctctactt tgtactttct actatatatc cttcgtctta atcaacctcc
     4621 aagaagcggc aataaacaaa attgggtaaa tcaatgccgc aatgtttact cctgttggtg
     4681 tgctttgttt gccaaaactg actgaatcga aactgaaagg ggatgagatg aattcgtcga
     4741 gactattgag tgggaaatac ggatataaat cgcttagcca agctcgtccg agaattggtt
     4801 ctacaatcca gtatagggaa ataaaaacca ggattgaaag tgcggaacga cgaacgataa
     4861 gacttatcaa tattgcagca ctcatcaacc caaagcaaac ccacatggct cgcagtaagt
     4921 aagtcatttc actaaaaata tcacccgaat tggctcctcc acctttggta aatccgaaca
     4981 ataatccgct cattaaagcc accaaaccag cgccaatgct gataacagca gccaatagca
     5041 gtttgctttt taggatttct tcgcgagaaa gtccgtcgat tacgtgttga cgcagcgtgc
     5101 gaaaggagaa ctcattcgat acgaggttga tcatcaacag tccgagcaca ggcgcgaacc
     5161 agctcgaaac gtaggcgatg taataccata cgtttggaaa ttgatacact acgctgaggt
     5221 taatcatcgg agtagtggtg aacttgtcga agccgtaata gactaagaaa atcagcagga
     5281 cgtaaaatcc ggtaaacacg taaaaggtgc ggtagctggc tagtttcagc cattcgattt
     5341 ggagtagcct tttcattttt gcaccagttt taagaattca ctttctagat ccgacttcac
     5401 tactaccaag tgattgagta ggattccttt ttcttgtagt tgtttgttaa tgctagctgc
     5461 atcaagatca tctgctaaat cggcaatcaa aaatccgttg cgatcttgaa tattactgat
     5521 tcctgtaaat gattgaatcg catttctcaa tgcagaaagg tcatttgatc ctaattcaat
     5581 gcgtttattt tcggtaagta gttgttccac ttttccgctg cttaacacat ttcctttttt
     5641 gaggatgaca acatcggtac aaaccttttc cacctcatta agtaagtggc tagctagaac
     5701 gatggttttt ccattggctt tctgacgaag tattaactga cgaatctcgt taattccctg
     5761 aggatcgagt ccgttcgttg gttcatctaa taccagtatt tctgggtcac atagcaatgc
     5821 tcctgcaatg gctaggcgtt gtttcatccc aagtgaaaag gttttgaacg catcattttt
     5881 acgatcaaac aattccactt ctttcaaaac cacatcaatg cgagaaagct cacagccttt
     5941 aatttttgca acaatctcta aattcttcca agcacttagg tatggataga aattaggtgt
     6001 ttcgattaag gcgccgatgc gtttacgggc ggatgaaaat tcttcatttc caaaccagct
     6061 gaaacttcct tggtcggcaa tcaatattcc agttaaacat ccgagtagcg tggtttttcc
     6121 gctaccgttg ggtccaagta atccgaacac ctgtcctttt tctacacgaa aactcacccc
     6181 gttaaccgcg gtgttttttc cataccgctt cagcagtgaa tcgacttcga gtaaggtcat
     6241 ttaatacgaa atgagatcaa gtaattgctg ttcgaaacgt cctttaggca agaaattctg
     6301 ctccaatacc gaagaaaaag gcactggagt gtcgagactt gctgaacgaa caactggcgc
     6361 atcaagatac tcaaaacatt tttcactgat acgagcggcg atttcacctc caattccacc
     6421 tgtgagacag tcttcatgca ggataattac gcgtcctgtc ttcttcactg aagctaaaat
     6481 tgcttcttca tcgaaaggta acaaggtgcg taaatcgagt aagtcggcat tgatttctgg
     6541 gtgcttgttt aacacatcca aagcccaatg aacgccaaaa ccataggtaa tgattgacac
     6601 atcttttcct tcgcggagca gattagcttt accaatttcg atagtgaaat aatcatcatt
     6661 tacaggttcc gaaatgctac gataaagcgc tttgtgctcg aagaacatga caggattcgg
     6721 atcttcaaat gaagccagca acaagccttt tgcatcattc gggaaagccg gataaactac
     6781 cttcaaacct ggtgtatgcg taaaccaagc ttcattactc tgactatgaa acggtccagc
     6841 agccacgcct gcacccgtag gcatacgaac aactacatcg gcatgttgtc cccagcggta
     6901 gtggctttta gccagattat ttacgatttg attgaaacct tctgtgacga aatcggcaaa
     6961 ctgcatttcc atcatggctt tcataccatt gattgaaagt ccgagactac ttcctacgat
     7021 tgcagcttca cacagaggcg tattgcgcac acggtctttt ccaaaacgtt cgacaaagcc
     7081 ttcagtaatt ttgaaaacac caccatattc tgcaatatcc tgtcccatga gtacgagatt
     7141 aggatgtttt tccatagcga gcgctaaacc gtctgatatg gcgtcgataa agcgtttgtc
     7201 ggttttcgac tcagaagctg gagattgtgt tgtgctcacg aatggagcat acacatcgga
     7261 tatttcgtaa gcagtgtcgg gtgtaatttc gggttcggca aaagtgatgg caacaccttc
     7321 ttcgattgtt tttttgaact cggcacggat gctagcaagc gattcatctg ttagcacgcc
     7381 ttcttgtaag agccaagctt cgtagttcac tactgggtct ttctttcccc atagttcgaa
     7441 taattcctga ggaacgtatt tagttccgga ggcttcttcg tgtccacgca tacggaaagt
     7501 catgcattcg agtaaccaag gacgtggatt tttctgaatg tcggcgcgga tttcctgcac
     7561 ggcatcatac acatcaagca cattgttgcc atctacttgt cgagctggaa tgccgtatcc
     7621 cggacctttt tcggtaaagg agttaaagcg gaactgttcg ttgctaggcg tagaaagtcc
     7681 gtatccgtta ttttcgatga tgaagattac tggtaaatcc cacacagcag ctacgtttac
     7741 cgactcgtga aaatctcctt gactggcgcc accatcaccc gtaaaaacag cagttacgtt
     7801 gttttctttt cgaagcttgt gtgcgagtgc aataccatcg gctagtccaa gttgaggtcc
     7861 gaggtgagaa atcatcccaa caactttgtg ttcttttgag ccaaagtgga aagaacgttc
     7921 gcgacctttc gagtagccat tgaatttacc ttgccattgg gtgaaaagtt ggtggaaagg
     7981 catgccacga ccagtaaaaa tggctaggtt tcggtgcatg gggaggatgt attcctcttt
     8041 atccatggct acgacagttc caacggaaat ggcttcttgt ccgattccag agaaccattt
     8101 agagattttt ccttggcgta ggagattgag cattttttcc tcgatcattc ggggataaac
     8161 cagctcttta tatatatgaa gtaaaagttc gtcgcttttg ccgttgcgct tgaagcttaa
     8221 ttgcatgaat tcgggatttg ggctgcgaaa ttagggcaaa ttcctaactt tgactcatgc
     8281 aaacggtctt ggtttttgtg attgttgggt tggcagcgat ttggttggga agaagattgt
     8341 ttttttcact tcgtccagcc aagaaggata aaggctgcga ctcatgtcat tgatgtaggg
     8401 ataaaacaaa aaatcccgca caaggcggga ttttttttca gttcttaagt actgattagt
     8461 gagcagcagg agctggagca gcagcagcag cagcagtgtc aactttagca gttgtgtcag
     8521 cagcagcagc agcagcagca gcgtcagctt cagctttagc aattgaatca gctttagcag
     8581 ctgcagcaat tgagtcagct tttgcttttt cagcttcagc attgtttcca ccgcaagaag
     8641 cgaaaagggc tacacctaca gtaaggatgg cgaaagttac ttttttcatg atgttgtttg
     8701 tttttatttt gattgattca gcgggacaaa attaaaaaaa ataaaaaacc aacaagcaaa
     8761 atcaaaaaaa atgttaatcc cttattgtga ttgactaaaa aggtggtttt ccgattaaaa
     8821 cagtcataaa gttagtgtca gacttactca aaatcggcgt tttcctgcgt atataaattt
     8881 tggtgttttg aatattcatg tatttttgct cccaaaccta taaacagcag acatgaaaaa
     8941 atttcaaatg aatcttgcca aaaacttacg attgatcggt ttaacaattt tagccggtac
     9001 aatgaccttg acttattcag gttgtaaaaa agaaggatgt actgatacag atgctaacaa
     9061 ttacaatgaa gaagcagatg aagatgatgg tacatgtact tacgatagcg aaaaattcat
     9121 cggagtttac acatcgactt ctccatgtgt agctggtggt tcttggaaca ctactatttc
     9181 agcttcttct tcaagcaaga aaaaagtagt tgttactaat attggtggtt taggttctag
     9241 cgctactgct acagctgaca tcgatggaag tacaatcaaa atcaatagcc aaagcgtaat
     9301 tgattctgat ggtgattctt ggacagtatc ttctacatct ggtacacttt caggaacttc
     9361 aatcaactta actattacct atactttcgg ggttagtaat ttgacttgca gcgaaacttg
     9421 gaataagcaa taagctcatt cgaataaatt taaaagggct gtccatttgg gcagcccttt
     9481 ttttatggat ataagtgatt ttttacaaat gatattcctg caatgaactt ataacttcgt
     9541 acagttattt ctttaaggtt tgatataata cttagggttt agttcaatcg agtttattag
     9601 tttggttaaa acaactcgtt aagctttggg atttatgaat gggatagggc aaaaaaaaac
     9661 cttcccgatt aagagaaggt tttttaatga tatgcagatt aggaattatt tccctttcat
     9721 tgcgtctttc atagatttag gactagttgt agatccacct ttttgatctt taacggcatc
     9781 cttcatgtta gttggtgtat ttgatccacc tttaacagca tccttcatgt ttttaggatc
     9841 ttctttctta ggctcttctt gtttaggagt tgtgcctttt gttggcttac ttccttttac
     9901 aggagcagca gtagcagctt cagcagcagc agcttcagca gcagcgattg aatcaagacg
     9961 agcttgttct ttcattgctg aatcgataga atcctgacgt gctttttcaa cagcagcgat
    10021 tgaatcctta gtagcttgtt cttttttagc cagttcttct gcgcttggac cgcaagcagt
    10081 cagggttgca agagcaactg cagaaaaaat gaaaagcatt tttttcatgt tgtatgggtt
    10141 tttgaatatt cgagggcaaa aatagtaatt ctaatgttgt caaacctaaa aacaaatgaa
    10201 ttgggattaa attctagtgt aaagcacacc tttacttagt gtcagctgct gatagattga
    10261 tatttgctct gtttatctgt attttattta ctttcattta tatatttcct cctttgaagc
    10321 atccattctt ccccacttcg atacttatta ttctagttta tgtagccttg gcacttcctt
    10381 ttagtagctg tgagcccgac cccggacttc catcaactga ttctattata actgtcgata
    10441 cgctaccgta taattacatt tataagtcat atgtaattaa taattggatg tgtaatgtgt
    10501 caggatatca ttatactcct tatcaactag agattaaaca ggatacgatt aattttaacc
    10561 aagttcttgt ttcaaacttt gcaaatataa gtgggcaagt tgtaggtgtt ttaagcagtg
    10621 ataattctgc ggattctatc ataattactg ttccaaccca atacttttct tatccatcat
    10681 caagcaatcc taacatttat gatacattgt ttgtcaatgg taatcttttc gtttatcccg
    10741 attcaataat aattcagcaa cacacaaaaa taaattcctt ttatggggat gtttgtattg
    10801 gttttggaaa tccccaataa tcagtttttc caccatataa gtaccatcga atcattcttt
    10861 gcgtttaatt tgccgattca taaactgcca tgaaatcgtt tagtctgatt tttctctcgc
    10921 tttgtcttgc acttagctcc tgcgatcgtg atggttgcac cgatccgaat gccaacaact
    10981 acgacgataa atacgaaaac gacgatggtt cttgtcgtta cgattcagat ccattcgatg
    11041 gtcgctggga agcacacgat tctatcttag cctttggtgt gtatactgct gatgaagtgc
    11101 gtacgctcga cattcgcatt cttactgcca atcgtgctaa agtgaagttt ttttggcgca
    11161 atgctgatgg aacttattcc gatacactcg aagccaattc caagcctaat acgctttc
//
LOCUS       DAOOQW010000060        11078 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_7371_length_11078_cov_5.757870, whole genome shotgun sequence.
ACCESSION   DAOOQW010000060 DAOOQW010000000
VERSION     DAOOQW010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 11078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11078
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7371_length_11078_cov_5.757870"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..974
                     /locus_tag="PK281_05255"
     CDS             <1..974
                     /locus_tag="PK281_05255"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HOY48788.1"
                     /translation="HLYSALCSSKVICLEFIPGIKINKIEALQAQGYDLKAIARKGFD
                     AYFKQIFEWGYFHADPHPGNLLVLPGEVIGILDFGMVGRLSEKDRSALVEFIIGLGSD
                     DTARIVKNIEKLQGSEIADKPALEKDMEAFIEEFGTQAVKDIDLNEALNRGRLLINKH
                     KLKLNPDLFLLLRTVSMLEGLGMSLDPDFRSLEVIKPYAFKLLQKNLNPKNLFKSRKL
                     ISALADLASLAASFPADARKIVDKLKNDQVKVQIDDRATRHLAREVNLAGKRIGNSLV
                     FMSLFAGGCYFSSLPLPELFWHLNTPALVCLGAAFLVLSNLWYLRRK"
     gene            complement(976..>1263)
                     /locus_tag="PK281_05260"
     CDS             complement(976..>1263)
                     /locus_tag="PK281_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HOY48789.1"
                     /translation="ALFEVEKPLTTLGIGVDALPVHIRYSPVLTGNNLGQLGNVEHIP
                     SEIECREFKNSGELELLLAQVTESDFASHRLAQQLLAQGEIQKAWKVLLAS"
     assembly_gap    1264..1363
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1364..1725)
                     /locus_tag="PK281_05265"
     CDS             complement(<1364..1725)
                     /locus_tag="PK281_05265"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006988767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HOY48790.1"
                     /translation="MLTVDPKEVSIAKFHSILLGAVGPRPIAFASTIDEDGRPNLSPF
                     SFFNAFSANPPVLVFSPARRVRDNTTKHTLDNVLRVPEVVINVVNYEMVQQMSLASTE
                     YPEGVNEFEKAGFTQLAS"
     gene            1901..2269
                     /locus_tag="PK281_05270"
     CDS             1901..2269
                     /locus_tag="PK281_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010517543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3276 family protein"
                     /protein_id="HOY48791.1"
                     /translation="MDYNEQSDREEIYSRPVRAGKRTYFFDVKSTRGDDLYLTITESK
                     KRFKDDGTFFYEKHKIFLYKEDFEKFAEGLNDAIGFITDNNVSVPQTSAPESYTASAT
                     TENETSSWSNVEFDDLDGKA"
     gene            complement(2272..2760)
                     /locus_tag="PK281_05275"
     CDS             complement(2272..2760)
                     /locus_tag="PK281_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013620911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HOY48792.1"
                     /translation="MDFRVEIHSINHIAYLSWFITNHSCMDQIVSHAPQAIRIAQIAF
                     ACFFSVLFLQSGLDKLMNFRENLSWLVNHFSKTFLSAVVPVLFILLTLCELSAGVVSG
                     YGIIELIIHKSSTVAYYGAALSVLALLFLFFGQRIAKDYGGASGIVSYFIASVFALIL
                     MA"
     gene            complement(2802..3323)
                     /locus_tag="PK281_05280"
     CDS             complement(2802..3323)
                     /locus_tag="PK281_05280"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOY48793.1"
                     /translation="MTTILSATNRLNSMTRRVTEKYAELMHERGFPIQVEFMEDIPVN
                     TTSDAIYRKGDNEMRIYGHRIFNSADRFVIISPEYNGSIPGILKLMIDSCDPEIFKGK
                     KFALVGVASGRAGNLRGMDHLTDILHYLQAEVYSLKQPISQVFKLLDDDKSLSDPATL
                     QVLMKQVDGFQRF"
     gene            3363..4046
                     /locus_tag="PK281_05285"
     CDS             3363..4046
                     /locus_tag="PK281_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010520081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HOY48794.1"
                     /translation="MNLTYFGHSCFLIETKAAKILFDPFIRNNELAAHIDPLKIKADY
                     ILISHGHFDHLSDAEEIALNTNALLIANWEIYSWYLKKGLKNLQPMNSGGKLKTSFGS
                     IVMTQAIHSSSLPDGSYGGNPNGFVLHSDEMSVYYSGDTDIFQDMQLIAKFNKPKYAL
                     LPIGNIFTMDIHTAIESAKLLQVNRVIGLHYDTFPPIQINKLEAIRAFAAENIELILL
                     EIGETKSLD"
     gene            complement(4043..4675)
                     /locus_tag="PK281_05290"
     CDS             complement(4043..4675)
                     /locus_tag="PK281_05290"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HOY48795.1"
                     /translation="MRNRPRLRKFLIITGTLLLTLLIVYVARRPIMRTVGNYLIEEDE
                     LSTCEALFVLSGNPNDRSLEAARLLKAGYAPYIVCTGEVVPRLFEVIGDSTTDEADLS
                     RISLLDAGVPDSKIELLHIGTSTREESDAILAYCKNKGLKKIMVVSDKFHTNRINYAF
                     RSIYDDAGVEMVLRGCPSTAYSEEKWWAEESGLLMVNNEYVKLVYYHLRH"
     gene            4726..5427
                     /locus_tag="PK281_05295"
     CDS             4726..5427
                     /locus_tag="PK281_05295"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOY48796.1"
                     /translation="MLSYTKITLNIVLSYFRPEAIIMNRLFLLVFILTGMHSFLLAQA
                     PARKVIQFSGVVVSGDSLQPVPYTNIADKSTRHGTVSDYYGFFSFVAQENDTIEFSSI
                     GFKKAQYIIPQSLTESRYSIIQVLNRDTIELATTVIYAWPTREQIYEYFLSAPIPDDD
                     VERARKNLAQEKITDMAINMPMDANMNYRNSMQNYNTRLYTAGQIPMNNLLNPIAWAK
                     FVEAWKKGDFKRKSN"
     gene            5472..7988
                     /locus_tag="PK281_05300"
     CDS             5472..7988
                     /locus_tag="PK281_05300"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HOY48797.1"
                     /translation="MQRLLLLFLLVVSTGINAQNATITGRVTGEQGNLLAGVIVYRIG
                     DLSKAVTSDSEGRFKLTVPADQQLYIVYKLTGYESDTLALRLKPGEQKFIDRYLSMKV
                     KDVSTFEVIDQGDRQTGLQRLDPLIVNTLTGPGDAITMTLKTLPGVYSNSELSSQYSV
                     RGGNYDENLVYVNDVEIYRPFLVRAGQQEGLSFANPAMVENLKFSAGGFEAKYGDKMS
                     SVLDITYKRPKGFGGSASASILGGNLQLEGATKNYRLSGMVGARYRTTQYLLNSLDVN
                     GQYRPSFTDVQGYVLYNITEKWDIDFLGNYAVNNYRVFPEDQTTEFGNINQALQFKVY
                     FDGKEQSRFATSMGAMTTTLRPTKKLKLKLITSVFQSSESEKSDVAGEYYINELDRDL
                     GSEDFGDVAFNRGIGAYQTFIRNELHYTVANIEHKGYYENGDHYILWGFRAQNEQIND
                     HIKEWRMIDSAGFSMPISQTIDQLYGPDSIVLNTYLKSSTSLNSMRYQGYVQDNYKWR
                     KDSIDFALTGGIRANHWTINGQTTVSPRLVFSVKPLSWKQDFTFRFAAGHYAQPPFYR
                     ELRGFDGSINKNLKAQESWHFITGADFTFKAWGRPFGFYAEGYYKILRNLVPYEIDNV
                     RVRYYADNISDGYAAGIDMKVNGEFVKGVQSWASMSIMKTQEDLRNDYYYIRYNSEGE
                     KIIPGYSTNTVAVDSQRVEPGYIPRPTDQRFNFSLFFQDYLPKNESISMQITLVIGSR
                     LPLGPPDFNRYRDTLRIPPYRRVDIGFSKQFIRAGKPLPKTSPFHNLKTLTLSLEVFN
                     LLGVNNTVSYTWIKDVTNRTYAIPNYLTSRLLNLRLQASF"
     gene            8063..9655
                     /locus_tag="PK281_05305"
     CDS             8063..9655
                     /locus_tag="PK281_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48798.1"
                     /translation="MKTILSHNHTSKSAFLVIGLLMLIVWIKTQVFFHFSILYTDCDQ
                     AVLWQTLHDISKGNFFQPCFYGQAYNSGLESILAWPFYALGMKEYYALPLVSILISLL
                     PYLILALLFIQKKDWWAAIFTLLLYLWLPPFYDLFTALPRGFVTGLFACSFLLPAWFF
                     PENKKVLALAGFTLPFAFWLNYNTVIFIVPFLVKLALAHYKKPISFFIFYFILTVPGL
                     VWILFTRNFYLTHPHYLVHSIEISKFKWKYIYEFWTNWNNWLGYLTPGLWKFSQLVFI
                     FLLIPLITFIRTKNREMLLIYLSFIGILVFSTGFEKIKDGVTSDIFFPLSRMFLCFTL
                     MLILFLAPIFSAWIKRFQASWISLLAIVALSSMITIQKHNELALRSKWDFINKLEIRV
                     QQMPIEHVFYKCNELQQISNQYKPDLIVFAINETYEFNYLLLPYACPCLVDDLPTTIV
                     KSYDRRGHLITKIKRIKFHRILVLNKQETLEKRVQSDPKHYRKIKSRENSYFLFESKE
                     GITWLDFNFSISNYQERIRFRP"
     gene            complement(9652..10161)
                     /locus_tag="PK281_05310"
     CDS             complement(9652..10161)
                     /locus_tag="PK281_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48799.1"
                     /translation="MKTDISFIARYFKAELLESLVFIAIGIITFSIALAAWFKFKTPF
                     LSGLAWPFLLIAFIQLTVGLSVYFRSPKDIIRVEEIAFNSPELSQQEIPRMETVMKNF
                     VIYRYVEISLLVIGIGLLVFLSSAFARGIGLGLLIQAALMLGADYFAEKRGSEYQADL
                     ISHMDKMKN"
     gene            complement(10163..10999)
                     /locus_tag="PK281_05315"
     CDS             complement(10163..10999)
                     /locus_tag="PK281_05315"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOY48800.1"
                     /translation="MNKYSTKMNQTVIITGASTGIGRAAAEYFASKGWNVAATMRNPE
                     KYPELKSISGIKTYVLDVTNPSSIDAALSQIQNDFGRIDALVNNAGYGVDGVFEAMSD
                     AVIEKQFDTNVFGLMRMTRAIIPIFRNQKSGTIIQIASVGGRVAFPLFSIYHGTKWAV
                     EGFSESLRYELEPLGIKMRIIEPGAIKTDFYNRSRVFVKPETTNQYDEFVAKCEKTSS
                     NAGNTGASALSVAKVIYRAATDGGNRLRYATGFPAPMLLWMRKWMPDSWFKFVVKSSY
                     GI"
BASE COUNT         3424 a         2130 c         2028 g         3396 t
ORIGIN      
        1 cgcatttata ttcggcatta tgctcttcca aagtaatttg tttggagttt attcccggaa
       61 ttaaaatcaa caaaattgaa gcattacagg ctcaaggata cgacctgaaa gccattgccc
      121 gtaaagggtt tgatgcctat ttcaaacaaa tattcgagtg gggatatttc catgctgatc
      181 ctcatccagg aaatctgtta gtattaccgg gtgaagtcat tggaattcta gactttggaa
      241 tggtaggacg attgagcgaa aaagatcgtt ctgccttagt cgaatttatt atcggtttag
      301 gaagcgacga taccgcccga attgtaaaaa acattgagaa acttcaaggt tctgaaatcg
      361 ccgataaacc tgcattagag aaagatatgg aagcctttat cgaagaattc gggacacagg
      421 cagttaaaga tattgatctc aacgaagcgt taaatcgtgg tcgactatta attaataaac
      481 ataaacttaa actcaatccc gatttattct tgttgcttcg aacagtctct atgctcgaag
      541 gattgggaat gagtttagat ccggatttta gatcgctgga agtaattaag ccttatgcat
      601 ttaaattact tcagaaaaat ctaaacccga agaatttatt taaaagccgg aaacttattt
      661 cagcattagc tgatttggct tctttagcag catctttccc cgctgatgca cgaaaaatag
      721 tagataagct gaagaacgat caagtaaaag ttcaaatcga cgatcgagca acacggcatt
      781 tagcaagaga agtaaatctg gcaggaaaac gcatcggcaa ttcgctcgtg tttatgagtt
      841 tgtttgcggg cggatgttat ttcagcagtt tgcctttacc cgaattattc tggcatttaa
      901 acacaccagc acttgtttgc ttaggtgcgg cattcttggt tttatcgaat ctttggtatt
      961 tacgacgcaa gtaaattagc tagcaagaag aactttccat gccttttgaa tctctccttg
     1021 agccagtaat tgctgagcta aacggtgaga ggcgaaatca ctttcagtca cttgagcgag
     1081 caaaagttct aattcacctg agtttttaaa ttcacgacat tctatttccg atggaatgtg
     1141 ttcaacattt cccaactgcc ctaaattatt ccccgtcaat accggagaat accgaatatg
     1201 tacaggtaat gcatctacac caatacctaa agttgtaagc ggtttttcta cttcaaacaa
     1261 tgcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntctgatg ccaattgggt
     1381 aaatcctgct ttttcaaact cattcacacc ttccggatat tcagtactgg caagcgacat
     1441 ctgctgtacc atttcgtaat taaccacatt tattactact tcaggaaccc gaagcacatt
     1501 gtcgagcgta tgtttagttg tattgtcgcg aacccgtctg gcaggcgaga aaactaatac
     1561 aggaggattc gcactaaatg cattgaagaa actaaatgga ctcaggttcg gacgaccatc
     1621 ttcatcaata gtactggcaa aagctatggg gcgaggacca acagcaccaa gcaaaatact
     1681 gtggaattta gcaatcgaaa cttctttggg atcaaccgtt agcatgagaa aattttgaat
     1741 tcaaaagtag gagatagaat tacaaataag gtgtaatgag atagaaaata gaatcaatat
     1801 ggattagcgt gtttttttac tcgaaaagtc tgagggataa gaattttaat aaatttgtcc
     1861 agctacctaa atcaaacgca ttttttataa cgtagaaagc atggattaca acgaacaatc
     1921 ggatcgtgag gaaatttact ctagaccagt aagagcggga aaacgaacgt atttttttga
     1981 tgttaaaagc acccgtgggg atgatttgta cttaacaatc acagaaagca aaaaacgctt
     2041 taaagatgat ggtactttct tttacgagaa gcacaaaatc tttttgtaca aagaggattt
     2101 cgagaaattt gctgaaggct taaacgatgc aattggtttt atcaccgata acaatgtgag
     2161 tgttccacaa acatcagccc ctgaaagcta caccgcttct gcaacaaccg agaatgaaac
     2221 atcttcttgg tcgaatgtag aatttgatga cttagatggc aaagcttgat ttcaagccat
     2281 cagtatcaaa gcaaatacac tagcaataaa gtaagaaaca ataccgctgg ctccaccata
     2341 atctttggca atacgttgtc cgaagaatag gaacaataaa gctagcaccg ataaagcagc
     2401 accgtagtaa gctactgtgc tgcttttgtg tataataagt tcgataatac catatcccga
     2461 aaccactcct gcagataatt cacaaagcgt gagtaaaata aacaatacgg gtactacagc
     2521 actcaagaat gttttagaaa agtggttaac tagccaagat aaattctccc gaaaattcat
     2581 cagtttgtct aggccagact gaagaaataa cacagaaaag aagcaagcaa atgctatttg
     2641 agcaattcga atggcttgcg gggcatgact aacaatctga tccatacacg agtggttggt
     2701 gatgaaccaa gataagtagg caatatgatt tatcgaatgg atttcaacac gaaaatccaa
     2761 cagaataaat caagtaactg aaaagcagtc gattaaaaaa tctagaatcg ttgaaaacca
     2821 tccacctgtt tcatcaatac ttgcaaagtg gcgggatcgc ttaatgactt atcgtcatcg
     2881 agtagtttaa acacttgtga aataggctgt ttcaaggaat aaacttcggc ttgtaggtaa
     2941 tgtaaaatat ccgtcaaatg atccattccc cgcaaattcc ctgctcgtcc agacgcgact
     3001 cccactaaag caaatttctt ccctttgaat atttcagggt cgcatgaatc aatcattaac
     3061 ttcaaaatcc cgggaatgct tccattatac tcgggtgaaa taattacaaa acgatctgcc
     3121 gaattaaaaa tacgatgtcc atagattcgc atctcattat ctcctttccg gtaaatagca
     3181 tcacttgtag tatttactgg aatatcctcc ataaactcaa cctgaatcgg gaaaccacgc
     3241 tcatgcatta actcagcgta cttttccgtc acacggcgtg tcatactatt taatcgattg
     3301 gttgctgaaa gtatcgttgt cattatttcc cttcgtagta aattgctcaa tatttttttc
     3361 caatgaacct aacatacttt ggccattctt gttttcttat tgaaacaaaa gctgcaaaga
     3421 tattattcga tccgtttatc cgaaacaatg aactagcagc tcatattgat ccgctaaaaa
     3481 ttaaagccga ttatattcta atcagccacg gacatttcga tcacctttca gatgctgaag
     3541 aaatcgcatt aaatacaaac gcattactaa ttgcgaattg ggaaatttat tcctggtatt
     3601 taaagaaagg tttgaaaaat cttcaaccca tgaatagtgg aggaaagcta aaaacctcat
     3661 tcggaagtat cgtcatgaca caagccattc attcgagcag tcttcctgat ggaagctatg
     3721 gcggaaaccc caatggtttt gtacttcatt cggatgaaat gagtgtttac tattcgggcg
     3781 ataccgacat ttttcaggac atgcagctta ttgctaaatt caataaaccg aagtatgcct
     3841 tattacccat cggtaatatt ttcaccatgg atattcatac agctattgaa tcggctaaac
     3901 tattacaagt aaatcgtgta atcggattgc attacgatac cttccctcct attcaaataa
     3961 ataaattgga agcaatacgt gcatttgcag ccgaaaacat agaattaatt ctgttagaaa
     4021 ttggggaaac taaatcttta gattaatgcc tcaaatggta gtaaactaac ttgacgtatt
     4081 cattatttac catcaataat ccagattctt cggcccacca cttttcttcc gaataagcag
     4141 tactcggaca tccacgcaat accatttcca ctcctgcatc atcgtaaata cttctaaatg
     4201 catagttaat tctgttggtg tggaatttat ccgaaaccac cataattttc ttcaagccct
     4261 tgttcttaca ataggctaga atggcatcgc tttcttcacg cgtacttgtg ccaatgtgta
     4321 acaattcgat tttagagtct ggtactccgg catctaataa agaaattcga cttaaatctg
     4381 cttcatcagt tgtactatct ccaattactt caaaaagtcg aggaactact tctcctgtac
     4441 aaacaatata tggtgcatat cctgctttca agagtcgtgc agcttctaat gaacgatcat
     4501 ttggattgcc acttaaaaca aacagtgctt cacaagtgct cagttcatct tcttctatca
     4561 agtaattacc aacagttcgc ataatgggtc ttcgagccac ataaacgata agtagtgtta
     4621 ataatagcgt accggtaatg attaaaaact tccggagtcg aggtcgattt cgcatatgcg
     4681 aacctacaat tttttaaaga tttcagtttg ttcagaaaac tgtttatgct ttcctatacc
     4741 aaaatcacct taaacatcgt tttgtcgtac tttcgccctg aagcaattat catgaataga
     4801 cttttcctcc ttgtattcat tctcacagga atgcactctt tcctgctggc tcaagctcct
     4861 gcgcgcaaag taattcagtt ctctggagtg gttgttagtg gtgatagctt acagcctgtt
     4921 ccctacacaa acattgctga taaatcgact cgtcacggta cagtgagtga ctattacggt
     4981 ttcttctcct ttgtagcgca agaaaacgat accatcgaat ttagcagcat tggatttaag
     5041 aaagctcaat acatcatccc acaaagttta actgaatcgc gatattcgat tattcaagtc
     5101 ctaaatcgcg acacaataga attggctact accgttatat acgcatggcc aactcgtgaa
     5161 cagatttacg aatacttttt aagtgcaccc attccggatg acgatgtgga acgtgctcgg
     5221 aaaaatttag ctcaggaaaa aattaccgat atggcgatca atatgcctat ggacgccaac
     5281 atgaattatc gaaattccat gcagaattac aatactcgtt tatatactgc tggacaaata
     5341 cctatgaata atctccttaa tcctattgct tgggctaaat ttgttgaagc atggaaaaag
     5401 ggtgatttta aacgtaaaag caactaacat tcgtagaaac taacatttag attcctacgt
     5461 aaatcgatct aatgcagcga ctgcttctcc ttttccttct cgtagtttca accggaatta
     5521 atgctcaaaa tgcgactatc acaggtcgtg ttactggtga acaaggtaat ttacttgctg
     5581 gtgtaattgt ttatcgcatt ggcgatttat ctaaagcagt cacctccgat tcggaaggtc
     5641 gtttcaaact tacagttcct gctgatcaac aactctacat tgtatacaaa ctaactggtt
     5701 acgaatcaga tacactagca ttaaggctta aaccgggcga acaaaaattc attgatcgat
     5761 acttaagtat gaaggtgaag gatgtttcca cctttgaagt gatagatcaa ggtgaccgtc
     5821 aaacgggctt acagcgctta gatcctttaa ttgtaaatac gctaactgga ccgggtgatg
     5881 caattacaat gactttaaaa accttacctg gtgtatattc taatagtgaa ttaagttccc
     5941 aatattcggt acggggagga aattatgatg agaatttagt ttacgtaaat gatgtagaaa
     6001 tttatcgccc ttttttggtt cgggcaggac aacaggaagg tttgagtttt gccaatcctg
     6061 caatggtgga gaatttgaaa ttttctgctg gtggatttga agctaagtat ggggataaaa
     6121 tgtcttcggt attagacatt acctacaaac gtccgaaagg ctttggcgga tctgcttcgg
     6181 ctagtatttt gggaggaaac cttcaacttg aaggagcaac taaaaattac cgtttatcag
     6241 gaatggttgg tgctcgctat cgtactaccc aatatttatt gaatagttta gatgtaaatg
     6301 gacaataccg tccatcattt actgatgttc aagggtatgt gctctataac atcaccgaaa
     6361 aatgggatat tgatttctta gggaattatg cggtaaataa ttatcgagtt tttccagaag
     6421 atcaaacaac cgaattcgga aatatcaatc aagcacttca atttaaggtg tattttgatg
     6481 gaaaagaaca aagtaggttt gctacatcaa tgggtgcgat gaccactact ttgcgcccta
     6541 ccaagaaatt aaaactcaaa ttaattactt ctgtttttca atcttccgaa tccgaaaaat
     6601 cggatgtagc aggtgaatat tacatcaacg aattagatcg agatttagga agcgaagatt
     6661 ttggtgatgt ggctttcaac agaggaattg gcgcctatca aacctttatt cgaaatgagc
     6721 tacattatac ggttgctaat atcgagcaca aaggttatta tgaaaatggc gaccactata
     6781 ttttatgggg atttcgtgct caaaatgaac aaataaacga tcatattaaa gaatggagaa
     6841 tgattgattc ggcggggttt tccatgccaa ttagtcaaac catcgatcaa ctttatggtc
     6901 ccgattcgat tgtattaaat acatacctca aaagttcaac tagcttgaat tcgatgcggt
     6961 atcaaggata tgtgcaagac aattataaat ggagaaaaga ttctatcgat tttgctctaa
     7021 caggcgggat tcgagcgaat cattggacta ttaacggaca aacaactgta agtccaaggt
     7081 tagttttttc agttaaacct ttgagctgga aacaagattt tacttttcga tttgctgcag
     7141 gtcactatgc tcaaccgcca ttttaccgag aattgcgagg ttttgatgga tcaataaaca
     7201 agaatttaaa agcacaagaa agttggcatt ttatcaccgg agccgatttt acttttaaag
     7261 catggggacg acctttcggt ttttatgctg aaggatatta caagattcta cgaaatttag
     7321 ttccgtatga aattgacaat gttcgagtgc gctattatgc cgataatatc agcgatggct
     7381 atgctgccgg aattgatatg aaggtgaatg gtgaatttgt aaaaggtgtt cagagttggg
     7441 caagtatgag tattatgaaa acgcaggaag atttgcgtaa cgattactac tacatacgtt
     7501 acaacagcga aggcgagaaa attattcctg gatattccac taatactgta gcagttgatt
     7561 cgcaacgagt ggagccgggt tatattcctc gccctaccga tcaacgtttt aattttagct
     7621 tattcttcca agattattta ccaaaaaacg agagcatttc gatgcagatt actttggtga
     7681 ttggttctcg tttaccgctt ggcccgcccg attttaaccg ctatcgtgat actttgcgaa
     7741 ttcctcctta tcgccgtgtg gatattggat tttcgaaaca gtttattcgt gcaggtaaac
     7801 cattacctaa aacttcgccg tttcacaatt tgaaaacact tacgttgagt ttagaagtgt
     7861 ttaatctatt gggcgtaaat aatacggttt cttatacttg gattaaagat gtgacgaatc
     7921 gtacgtatgc cattccaaat tatttaacga gtcgcttgtt aaatctacga ttgcaggcga
     7981 gtttttagat tgggatgatg ttgaattatt cgtgcgaacg tattttgata acgtattaat
     8041 tttacgctcc aattttcagc gcgtgaaaac aattctaagt cataaccata cgagtaaatc
     8101 tgcattcctt gttattggat tgttgatgct gatcgtgtgg attaagactc aagttttttt
     8161 ccatttctct atcctttata ccgattgcga ccaagctgtt ctttggcaga cgttacacga
     8221 tatttctaaa ggtaattttt ttcaaccttg cttttatgga caagcctata attcgggact
     8281 tgaaagcatc ttagcatggc cgttttatgc attgggaatg aaggaatact atgcgcttcc
     8341 actcgtttca atacttattt cactattacc ctatttaatt cttgcacttt tatttattca
     8401 aaaaaaagat tggtgggcag caatcttcac tttgctgctt tacttatggt taccaccttt
     8461 ttatgattta tttacggcat taccaagagg ctttgtaacc ggattatttg cttgttcatt
     8521 tttacttccc gcttggttct ttcccgaaaa taaaaaggtg cttgcattag ctggatttac
     8581 cttgccattt gccttttggc tcaattataa cacggtaatt tttattgttc cttttcttgt
     8641 aaaattggca ctagcacatt acaagaaacc tatttcattt tttatattct actttattct
     8701 cactgttcct ggcttagtgt ggatactatt tacacggaat ttttatttaa ctcatccgca
     8761 ttacctcgtt cactcgatag aaataagcaa gttcaaatgg aaatacatct atgagttttg
     8821 gacgaattgg aacaattggc tcggctactt aacacccggt ttgtggaaat tctctcagct
     8881 ggtttttatc tttctactaa ttcctctcat tacttttatt cgaacaaaaa atcgggaaat
     8941 gctcctgatt tatctgtcct ttataggtat tctagttttt tctactggtt ttgagaaaat
     9001 taaagacggc gttactagtg atattttctt tcccttatca cgcatgtttt tatgctttac
     9061 gctcatgctg attttattcc tagctcccat tttttcagct tggataaaac gttttcaagc
     9121 aagctggatt agcttgttag ctattgtagc attaagctcg atgattacca ttcaaaagca
     9181 taacgaatta gcacttcgta gtaaatggga ttttattaat aaactggaaa ttcgcgttca
     9241 gcaaatgccc atcgaacatg tgttttataa gtgcaatgag ctgcaacaaa tctccaatca
     9301 atacaaaccc gatttaattg tatttgcaat aaatgaaaca tacgaattca attacttatt
     9361 actgccttat gcttgtccat gtttggtgga tgatttacct actaccattg taaaatcgta
     9421 cgatcgccgt ggacatttaa tcacgaaaat taagcggatt aaattccatc ggattttagt
     9481 gctcaacaaa caggaaacac tcgaaaaacg agttcaatct gatccgaaac attatcgaaa
     9541 aattaagtcg cgtgaaaatt cttatttcct gtttgaatcg aaagaaggaa ttacttggtt
     9601 agactttaat ttctccattt ccaattacca agagcggata agattccgac cttaattttt
     9661 cattttatcc atgtggctaa ttaagtctgc ttgatattct gaaccgcgct tttcggcaaa
     9721 ataatcggct cctaacataa gtgctgcttg aatcaataat ccaagcccaa ttcctctggc
     9781 aaaagccgaa gaaaggaaaa ctaataatcc gataccaatt actagtaaac taatttcgac
     9841 atagcggtaa attacaaaat tcttcatgac agtttccatt cgaggaattt cttgctgaga
     9901 taattctgga gaattgaagg caatttcttc cactcgtatt atatcttttg gagaacggaa
     9961 atacacactt aatcctactg ttaattggat gaatgcgatg agcaaaaatg gccaagctaa
    10021 acccgataaa aagggcgttt taaatttaaa ccaagctgct aatgctatgg aaaatgtaat
    10081 aattccaatt gcaataaaga ctagactttc gagcagttct gctttgaaat aacgggcaat
    10141 aaatgaaata tctgtcttca tattaaattc cataactcga tttaacaaca aacttaaacc
    10201 aactatcagg catccatttg cgcatccata ataacatggg tgcaggaaaa ccagttgcat
    10261 aacgtaaacg atttccacca tcggtagctg ctcgataaat gacttttgct actgataatg
    10321 cagaagctcc tgtattacct gcattactgg atgttttctc acatttcgct acaaattcat
    10381 cgtattggtt agtcgtttct ggcttgacaa acacgcgact tcggttataa aaatcggttt
    10441 taatagctcc cggttcaatg atgcgcatct taattcctaa aggttctagc tcatagcgta
    10501 aactctccga aaaaccttca acagcccatt tggtgccatg atagatggaa aataatggaa
    10561 atgccactcg tccacctacc gaagcaattt gaataatcgt tcctgatttc tgattacgaa
    10621 atatgggtat gatagctcga gtcatacgca tcaatccaaa cacattggta tcaaactgct
    10681 tttctataac agcatcgctc atggcttcaa acacgccatc aacgccatat ccagcattgt
    10741 taactaatgc atcaattcga ccgaaatcgt tttgaatttg acttaatgct gcatcaatac
    10801 tcgaaggatt ggttacatca agcacgtatg ttttaattcc cgaaatcgat tttagttcgg
    10861 gatatttctc aggatttcgc atagtagcag cgacattcca tccttttgaa gcaaaatact
    10921 cggctgctgc tcttcctatt ccggtggaag ctccggtaat gatgactgtt tgattcattt
    10981 tagttgaata tttattcatt tacgtgtcaa aaaaaatcag cgttttacac tatcccaagc
    11041 caaggaaaat ggtgcatcta aattggttcg gtcaagct
//
LOCUS       DAOOQW010000061        10927 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_7503_length_10927_cov_4.573124, whole genome shotgun sequence.
ACCESSION   DAOOQW010000061 DAOOQW010000000
VERSION     DAOOQW010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 10927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10927
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7503_length_10927_cov_4.573124"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /locus_tag="PK281_05320"
     CDS             <1..714
                     /locus_tag="PK281_05320"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HOY48801.1"
                     /translation="TAIYKVRTSLYDLVKNIDSSAAVKRTIDVNAIMNLNDKLMKPQI
                     TFDITLPNADDNTLNLFKSQIYSEDELNKQVFSLVMFRGFMPNQGGATETTGLNGVGS
                     NASELLTSQLSNMLSQLSNDFNIGVNYSQGGVASKDQVSVNLQTQLFNDRISIDGNVG
                     TAGSVATSANTTNMVGEFNIEIKVTEDGAVRVRVFNRSNQYLLVSNDVPYTQGVGLFY
                     RREFETMGDLFKDKKKENN"
     gene            complement(711..1250)
                     /locus_tag="PK281_05325"
     CDS             complement(711..1250)
                     /locus_tag="PK281_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007844583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HOY48802.1"
                     /translation="MHPWHDITPGSSIPERVFAIIEIPKGSKAKYEVDKPSGLIKLDR
                     VLFSAVHYPANYGFIPQTYAGDHDPLDILVFSQIDFPPLTLVEATVIGVMRMVDKGET
                     DDKIIAVATQDISVNYLNDLSELPPHTMMEIQRFFEDYKRLENKNPVVIPTVLGKEEA
                     YAIIQESLVEYKKLFPKKD"
     gene            complement(1250..1768)
                     /locus_tag="PK281_05330"
     CDS             complement(1250..1768)
                     /locus_tag="PK281_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HOY48803.1"
                     /translation="MMWNDLLQITGRTVAVYLFIVLFLRIFGKKELAQLSVVDLVFIL
                     LISNSVQNAMVGPDTSLTGGLVAAGALFITNFIFKYLFRKLPFINRLIQGEPIMLIYK
                     GVVKNGNLKQVQMSLEELEAAIREHGVEHINQVDLAIFEVDGNISVLSKNFQHSTHHK
                     HMSHKPTLKSNI"
     gene            1846..2760
                     /locus_tag="PK281_05335"
     CDS             1846..2760
                     /locus_tag="PK281_05335"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HOY48804.1"
                     /translation="MFYFALIASFLIALVYIRFLRQMDVFEPEQWRYTWICFLLGVFS
                     VAGIFSVYYFFPQIMARPQDGNFAIRFQFHFFSVASLEELAKISPFLLLLMSKRVINE
                     SFDYIKYASLGAMGFAALENVLYFSRNLHIIEGRAFYTAVMHMFTSSLIAYCMYAFKK
                     RFNIPGFVVFCFAYPIAAGFHGLFNALISSGQTYLLGIGLVLCMLIAWGRMMNNTLNH
                     SEFFHSEPVQNKITMAGIQLLIGWGLVFLFAAFAIGILEGWSQSFVFLREGFVFGFIS
                     GAGLYLALARPRIRQGKWFPLIGRLKKE"
     gene            2924..4744
                     /locus_tag="PK281_05340"
     CDS             2924..4744
                     /locus_tag="PK281_05340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOY48805.1"
                     /translation="MKQNYTRRILDKLLIRNAQLPLHEDDLISRQRLNLFRTYSLTSF
                     LISLTITYQVISIFHWSDFIGIALILLTLIIAVNYFLLDRHLNYKVAYRIAILSTILT
                     LHVVTYYSGGIRNSGMIYLGSFILVTFMLLGNREGKIISVLSILNIIFFYIYTSTIGH
                     DVRNIIDSDSTGDMLNLDYLITYCTGTLLIYSLSNNLESSKNIVIAKVTEAKDSLEKK
                     NEELKKLSLVASSTDNSVLITDRFGFIEWVNEGFVRMKGFTFDEVIGRKSQDLFYGPL
                     TAKETIQRIQDLTSRNQSFSGEIQKYHKNGHTIWVQVTITPVMEEDGRVSRFIHVESD
                     ITERKKAEEKMAEYYTYLEKANKELDKFAYVVSHDLKAPLRAISNLTAWIEEDIGDRF
                     TEDASDHFKMLKGRVMRMEGLINGILDYSRADRVKSPNVNVNVKELIDEVVDTLVQDE
                     HVHIQITDKLPVLFTERMKLQQVFSNLISNAIKHSDKDETIINIDCKEEESDFVFSIE
                     DNGPGIDEQFHEKIFVIFQTLQARDTFESTGVGLAIVKKIVEESGGSIRVESEVGKFT
                     RFVFHWPKQSSSSFRPFQFTLNQPAATQSSNSASVSTLAS"
     gene            4741..4929
                     /locus_tag="PK281_05345"
     CDS             4741..4929
                     /locus_tag="PK281_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48806.1"
                     /translation="MKSPLHIFAAKSFDKAPLPSVSPAQLKPAVSPIHTLSDKEKDEL
                     RKITELLEQLRYSDNPDA"
     gene            5187..6623
                     /locus_tag="PK281_05350"
     CDS             5187..6623
                     /locus_tag="PK281_05350"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY48807.1"
                     /translation="MMNSKILFALLGIIGLGKIVSAQTAVSGGIYTNTTWTLANSPYL
                     MTNNIVVFPGITLTIEPGVEVRVKENGGSGTQYYLETRGTINMVGTSDAPITFRADTA
                     LTTIGAWQGIIIKNSQGASINYNYVNIANAVSSFVYDGSVPSLIQLNKCNFSYNANAV
                     VVGIDLIAEDCVFLGNETAIYGWANFTFRNCVFDDNNSALFIYASSLDMNNCVVRNNS
                     LGIGINSGSINGTLVRNTLFENNILAYTNANNGLIDSCTFIGNTDAVTNTTYLTVTNS
                     TFNNNGTALQVGFGSQVNDCLIEQNQTGVAFGPISFGQPMPVIENNRICSNALYNIDN
                     RTDLNIFIPTNCFCTTDSTEIETKIFDGYDDITKGLVGYAIFDTSCSNVLRLINKFGV
                     PTSLSENEIQENINVFPNPVIDQLTILNNNSFSSCSLITMDGKEVLTEGLMAGNTQIS
                     MSQLPAGIYFLVLRGAGQQSRTKKVVHL"
     gene            6843..7292
                     /gene="umuD"
                     /locus_tag="PK281_05355"
     CDS             6843..7292
                     /gene="umuD"
                     /locus_tag="PK281_05355"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007648838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HOY48808.1"
                     /translation="MAALKLHSSNNLDIYSALSDTALDLPLVSEGISAGFPSPAMDFT
                     DLTIDLNKHLIKHPSSTFYGRVKGQSMKDEGINDGDLLVIDKSLQPADGKIAVCYLDG
                     EFTIKRIRIDKKECWLMPANELYKPIQVTAENDFLIWGIVTHVIKSF"
     gene            7292..8551
                     /locus_tag="PK281_05360"
     CDS             7292..8551
                     /locus_tag="PK281_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007648185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HOY48809.1"
                     /translation="MFALVDCNNFYASCERVFRPELIGKPIVVLSNNDGCVIARSNEA
                     KAVGIPMGAPAFEYEALFVQHQVHVFSANFPLYGDMSQRVMNILGEYTPDIEIYSIDE
                     AFLKLKGFERFDLQEYGKDMQRRVTKWTGIPVSVGIAPTKALAKIANRIAKKFPKETG
                     SSYLINTEEKRIKALKWVQIEDVWGIGRQHAKRLRLIGVKTAYDFTQLHDAWVQHHMS
                     IVGLRLKHDLQGSPTLDLELVQPKKNIATTRSFERNYTKLAELEERVATFAVSCAEKL
                     RKQKSCCNTIMVFIHTNGHRKDLPQYSRNIVVKLPFATNSGIELAKFAKQALKHIFRE
                     GYHYKKAGVLVMDFTPQDKTQLRLFDNSNALHIPLMTAVDKINAMCGQQKIRLAAQDQ
                     KRVWKMKQEKLSPRYTTKLSDIIIIHT"
     gene            8551..9153
                     /locus_tag="PK281_05365"
     CDS             8551..9153
                     /locus_tag="PK281_05365"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HOY48810.1"
                     /translation="MCFHSKQSKSAQELKARFKAEFLHEEQYTPGIYNGFQYPQTPVI
                     THLHPQTIELLNWGLIPAWAKDSSFRKNTLNARYETIGEKPSFRNITHNRCLVLADGF
                     YEWQWLDEKGKKKQKFEITTPNGEAFAFAGLWTEWVDKSTGEILKTYTILTTEANELM
                     SQIHNSAKRMPIIVDARHEQEWLEGKPLIMGNADLIAAPI"
     gene            9270..9863
                     /locus_tag="PK281_05370"
     CDS             9270..9863
                     /locus_tag="PK281_05370"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOY48811.1"
                     /translation="MNNRQQLFEEVKQVVFQLLTDKLPPHLYYHCLDHTEDVIREATR
                     LAKAEQLSDQETLLVLLAAVFHDTGFIQSPQDHEARSCDIAREMLNNFPLTTDELDLV
                     CEAIMATKIPQVPMSKIAEVLCDADLDYLGRNDYDEISDRLFREIKHSNPNFSLSDWI
                     KLQDNFLKAHHYYTATSQRERNEIKAVNHQRVINQNN"
     gene            10114..>10927
                     /locus_tag="PK281_05375"
     CDS             10114..>10927
                     /locus_tag="PK281_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48812.1"
                     /translation="MRFLLTCILCLSSLLIVAQPHPYGCQHWRQSAGKGQALSSSASR
                     SLNESILRSDTFDILHYDIAIDLTAYGSQQISAQTEITFLPKMANLDFIRFDLVRLIV
                     DSVKNESGLLPFATDSQFVKVQYPSALTLNDTNSLTIYYHGHPYRDPVWGGFYFESGY
                     IYNLGIGLSTIPPNFGKVWYPCFDSFVERATYAYHVKTSGSYKAHCQGTFLGEQQLSG
                     DTVIRSYEFNQPLPTHLSAIAASNYFDLNFTHNGNYAPVPVKLTAKPADTSAM"
BASE COUNT         3206 a         2344 c         2080 g         3297 t
ORIGIN      
        1 accgcaattt ataaggttag aacatctctc tacgatttag tgaagaatat cgattcgagt
       61 gctgctgtta aacgtacaat tgatgtgaat gcaatcatga atttgaatga taaactcatg
      121 aaacctcaaa tcacattcga tattacactt cctaatgccg acgataatac actcaaccta
      181 tttaaatcgc agatatacag cgaggatgaa ttaaataagc aggtgttttc cttagttatg
      241 ttccggggat tcatgccaaa tcaaggtggt gcaaccgaaa caactgggtt aaatggagta
      301 gggtcaaatg caagtgaatt attaactagt cagctgagta atatgctttc acagttgagt
      361 aatgacttta atattggagt aaattatagt cagggcggag tagcttcaaa agatcaagta
      421 tcggttaacc tacaaacgca attattcaac gatcgaatta gtatagatgg gaatgttgga
      481 acggctggtt ctgttgcaac atcagctaat acaaccaaca tggtgggtga atttaatata
      541 gagattaaag taaccgaaga tggagctgtt cgtgtacgag ttttcaaccg ttccaatcag
      601 tatttacttg taagtaacga cgtgccgtat acacaaggtg taggtttatt ttaccgacgc
      661 gaatttgaga cgatgggaga tttatttaaa gacaaaaaga aggagaataa ttagtctttt
      721 ttcggaaaca actttttgta ttcaaccaag ctttcttgaa taattgcata cgcttcttct
      781 ttcccaagaa cggtaggaat tactacagga tttttgtttt ctaatcgttt ataatcttcg
      841 aaaaagcgtt gaatctccat catagtatga ggaggtaatt cggataaatc attcaagtaa
      901 ttcaccgaaa tatcttgagt agctactgct ataattttat catctgtttc gcctttatca
      961 accatgcgca taacaccaat aacagtggct tctaccaagg taagaggagg gaagtcgatc
     1021 tgactaaaaa ctaaaatatc gagtggatca tgatcgcctg cataggtttg tggaataaat
     1081 ccataattag cagggtagtg aacagctgaa aataaaaccc gatctagttt tatcaatcca
     1141 ctaggtttat caacttcata ttttgcttta ctgcctttag gtatttcaat aattgcaaat
     1201 actcgttcag gaattgaaga accgggtgtt atatcgtgcc aaggatgcat taaatattcg
     1261 attttaatgt tggtttatgt gacatatgtt tgtgatgggt actatgctga aaattcttac
     1321 tcaatacact gatatttcca tcaacctcaa atatggctaa atcaacttga ttaatatgtt
     1381 caactccatg ttccctaatt gcagcctcta attcttccaa actcatttga acttgtttca
     1441 ggtttccatt ttttaccact cccttataaa tcaacatgat tggttcacct tgaattaaac
     1501 gattgataaa aggtaatttc ctgaatagat atttgaaaat aaaattggtg ataaataagg
     1561 ctcctgctgc gactaaccca cctgtcaatg aagtatcggg gccaaccata gcattctgaa
     1621 ccgaattact tattagtaaa atgaatacga ggtctacaac tgaaagttgg gctaattcct
     1681 tctttccaaa tattcttaga aacaatacga taaataaata aaccgcaact gttcttcctg
     1741 taatttgaag caaatcattc cacatcatgt tgcaagctac atcattttaa gactttctaa
     1801 aatgactgat tttcattttt gtatatttca tttttatttc tccacatgtt ctatttcgcg
     1861 ctaattgcct cctttttaat cgcattggtt tacatccgtt ttcttcgtca aatggatgta
     1921 tttgaacccg aacaatggcg atatacttgg atttgctttt tacttggcgt cttttctgtt
     1981 gcaggtattt tttctgtgta ctatttcttt cctcaaatca tggctcgtcc tcaggatgga
     2041 aactttgcta ttcgatttca atttcatttt ttttccgtcg cttctctcga agaattagcc
     2101 aagatttctc cctttctcct tttgctaatg agcaagcgag ttattaacga atcattcgat
     2161 tacataaaat atgcatcact tggagctatg ggttttgcag cattagaaaa cgtattgtat
     2221 ttcagtagga atcttcatat cattgaagga agagcctttt atactgccgt aatgcacatg
     2281 tttacctctt cgttaattgc atattgcatg tatgcattta aaaaacgctt caatattccc
     2341 ggattcgtag ttttttgttt tgcttatccc attgcagcag gatttcatgg tttattcaat
     2401 gcactcattt ctagcggtca aacttacttg ctagggattg gacttgtatt atgtatgttg
     2461 attgcttggg gacgaatgat gaataataca cttaatcatt ccgaattctt ccattctgaa
     2521 cctgttcaaa ataaaattac tatggcaggt attcaactgc taattggttg gggacttgtt
     2581 ttcttatttg cggcatttgc cattggtatt ttagaaggtt ggagccaaag tttcgttttt
     2641 ttgcgtgaag gctttgtttt tggatttatc tcaggagctg gtttatactt ggctttagcc
     2701 cgtccgcgca ttcgacaagg aaaatggttt cctttaatcg ggcgattgaa aaaagagtaa
     2761 cttcatactt acattttgta gtaatttcta ttggtcacat ttcgtgtaag atgtaattga
     2821 agattgacct cttttacatg tttacagaag taactattta ggggtatgtt ccgaataaac
     2881 tgctgagaca catatcataa ttgtctctta taacatctgg gaaatgaaac aaaactacac
     2941 caggcgtatt ttagataaat tgctcatccg caacgcgcag ctacctttgc acgaagatga
     3001 tttgatttcg agacaacgac tcaatctgtt tcgtacctat tcgcttacgt cattcctgat
     3061 ttctctaaca attacctatc aggtaatttc catttttcat tggtccgatt ttatcggaat
     3121 tgccctcatt ctacttactt taatcattgc ggttaattac tttttacttg atcgacacct
     3181 caattataaa gtagcatatc gaatagccat tctgagtact atacttactc tacatgtggt
     3241 tacttactat tcaggaggta ttcgaaactc gggaatgata tacctcggct cctttattct
     3301 ggtcactttc atgttgctcg gaaatagaga agggaaaatt atttcggtgc tcagtattct
     3361 caacattatt ttcttctata tctacacctc tacaattgga catgatgtaa gaaatatcat
     3421 cgatagtgat tctacaggtg atatgcttaa tcttgattat ctcattacct attgtactgg
     3481 tacacttctc atttacagtc tttccaataa tctcgaaagc tctaaaaaca tcgttattgc
     3541 taaagttacc gaggcaaagg attcactcga aaagaagaac gaagaactca aaaaactctc
     3601 tcttgttgca tccagtaccg ataactcggt tctcattact gatcgttttg gttttatcga
     3661 atgggtgaac gaaggttttg ttcgaatgaa gggatttaca ttcgatgaag ttattggccg
     3721 caaatcgcag gatctcttct atggtccgct taccgctaag gaaacgatac aacgtattca
     3781 agatttaact tcacgcaatc aatccttttc gggggagatt cagaaatacc ataaaaatgg
     3841 tcataccatt tgggttcagg ttaccattac tcccgttatg gaagaagatg gtcgggtttc
     3901 gcgtttcatc cacgtagaga gcgacattac cgagcgtaaa aaggcggaag agaaaatggc
     3961 tgaatactat acttatctgg aaaaagccaa caaagagctc gataagtttg cttacgtggt
     4021 ttctcacgac ttaaaagctc cacttcgtgc cattagtaat cttactgctt ggattgaaga
     4081 agacattgga gaccgtttta ccgaagatgc tagcgaccac tttaaaatgc taaaaggtcg
     4141 agtgatgcgt atggaaggtt tgattaacgg tattctcgat tattctcgtg ccgaccgagt
     4201 gaaaagtccg aatgtaaacg tgaatgtcaa agagcttatc gacgaagtgg ttgatacgct
     4261 tgtacaagac gagcatgttc atatccagat tactgataaa cttcctgttc tatttaccga
     4321 aaggatgaag ttgcaacagg tgttttcaaa cctaatcagt aacgccatta aacacagcga
     4381 taaagacgaa actattatca atattgattg caaagaggag gagagcgatt tcgttttctc
     4441 gattgaggat aacgggccag gtattgatga acaattccac gaaaaaatat tcgtcatttt
     4501 ccaaacatta caagcgcgcg ataccttcga aagtaccggt gttggacttg ctatcgtgaa
     4561 gaaaattgtg gaagaaagtg gcggttcgat tcgcgttgag tcggaagtgg gcaagtttac
     4621 ccgtttcgtc ttccattggc caaagcaaag cagttcttcc tttagaccat ttcagtttac
     4681 gctaaatcaa cctgctgcta cacaatcttc aaacagtgca tccgtttcaa ctttagcatc
     4741 atgaaaagcc cattgcacat attcgctgca aagtctttcg ataaagctcc tttgccctcg
     4801 gtttctccag ctcagttgaa accggccgtt tcgcccattc ataccttgag tgataaggag
     4861 aaggacgaac ttcgtaaaat caccgaacta cttgaacaac ttcgctacag cgataatccc
     4921 gacgcctaat tactccgttt gggataacca aatttgatta acggattgct ttagattaga
     4981 tgttttataa tagctattag catcaacgat tattctcaat tccccttttt tactttcccc
     5041 tttttacttt ctactttccc ccttttttac tctccccttt ttactttcta ctttatactt
     5101 tccccctttt ttactttttc cctttttccc tgattcatcc tgaacatttc taatttgcct
     5161 gtgtttatca tcgaaaaata tcatcgatga tgaatagcaa aattttattc gcacttctgg
     5221 gaattattgg attgggtaaa atcgtatccg cacaaacagc ggtaagcgga ggtatttata
     5281 ccaacacaac ttggactctg gctaatagtc catacttgat gaccaacaat attgtggtat
     5341 ttcctgggat aacattaacc atagaacccg gtgtagaggt gcgagtaaaa gaaaacggcg
     5401 gatcgggaac ccagtactat ctagaaaccc gcggtaccat aaatatggtt ggtacttccg
     5461 atgctcctat tacattcaga gccgatacag ctttaacaac aattggtgca tggcagggaa
     5521 ttattatcaa gaactctcaa ggtgccagca ttaattacaa ttacgtgaat atagctaacg
     5581 ccgtttcttc ctttgtatat gacggttcag tgccatcgct tatccaattg aacaaatgca
     5641 acttcagcta taacgcaaat gccgtggtgg taggaataga tttgatagcc gaagactgtg
     5701 tttttttggg taacgaaacg gccatttacg gttgggccaa ttttacattc agaaactgtg
     5761 tttttgatga taataattct gcacttttca tatatgccag tagtctagac atgaataatt
     5821 gcgtggttcg caataatagc ttgggcattg gcattaactc cggttccata aatggtacct
     5881 tggttagaaa tacactattc gagaacaaca tacttgcata caccaatgct aacaacggct
     5941 taattgattc gtgtactttt attggtaata ccgatgctgt aacaaatact acttacctaa
     6001 cagttacaaa cagcactttt aacaacaacg gcactgcatt gcaagtggga tttggttctc
     6061 aggtaaatga ttgcctgatt gagcagaatc aaaccggcgt agcctttggt ccaataagtt
     6121 tcggtcagcc tatgcctgtc atagaaaata accgtatttg ttcaaacgcc ttgtataaca
     6181 tcgacaaccg aacagatctg aacatattta taccaaccaa ctgtttctgc accaccgatt
     6241 caaccgaaat agaaacaaaa atctttgacg gctacgatga tataaccaag ggacttgtcg
     6301 gttatgccat attcgacaca tcttgttcta atgttctgag attgattaat aagtttggag
     6361 tgcccacttc gcttagcgaa aacgaaatac aagagaatat taacgttttc cctaatccgg
     6421 ttatagatca actgaccatt ttgaacaaca attcattcag ctcgtgttct ttaatcacga
     6481 tggatggtaa agaggttttg actgagggac ttatggctgg aaatactcag atcagtatgt
     6541 cacaattacc agcagggatt tattttcttg ttctcagagg agctggacag caaagtcgca
     6601 ccaagaaagt cgtgcatttg taaattagaa tgtctgttta ttttatttga cacattcaat
     6661 tcattcagct ctccaatttt ggcaatttaa gtgtgcatag attgatgccg aaataggcaa
     6721 aaaacatccg cctcgcgcgt gtcttttttc tacccctttc gtatcttgca caagtagcaa
     6781 ggaggagttc gttagctggt aaatgccagt aacacactag tttccgcctt gctcatacgc
     6841 gcatggctgc tctcaaatta cattcttcca ataacctcga tatctattcc gctctttcgg
     6901 atacagcact cgatttgccc ctcgtttccg aaggcattag tgctggattt ccctcgccgg
     6961 caatggattt caccgatctt accatcgatc tcaacaagca cctcatcaaa catccttctt
     7021 ccaccttcta cggtcgcgtg aaagggcaat ccatgaaaga tgaaggcatt aacgatggag
     7081 atttgctcgt gattgacaaa agtcttcagc ctgccgatgg gaaaattgcc gtgtgttacc
     7141 tcgatggcga attcaccatc aaacgcatcc gaatcgataa aaaagaatgc tggctcatgc
     7201 ccgccaacga actctataaa cccattcaag taaccgccga aaacgacttt ctcatctggg
     7261 gaatcgtcac gcacgtgatt aaatcattct aatgtttgcc ctcgtcgatt gcaacaactt
     7321 ttacgcttcc tgtgagcggg tttttcgccc cgaactcatc ggtaaaccca ttgtggtgct
     7381 gtcgaacaac gacggttgcg tgattgctcg tagcaacgaa gccaaagccg ttggaattcc
     7441 catgggcgcc ccagcattcg aatacgaagc gctttttgtg caacatcagg tacacgtttt
     7501 ctccgccaat tttccgctct atggcgatat gagtcagcgg gtgatgaaca tcctcggcga
     7561 atacacgccc gatatcgaaa tttacagcat cgacgaagcc ttcctcaaac tcaaaggttt
     7621 cgaacgtttc gacctgcagg aatacggaaa agacatgcag cgcagggtaa ccaaatggac
     7681 tggaatacct gtcagcgttg ggattgctcc taccaaagcg ctggcgaaaa ttgccaatcg
     7741 gattgccaag aaattcccca aggaaacagg cagttcctat ctcatcaaca ccgaggaaaa
     7801 acgcatcaaa gcgctcaaat gggttcagat tgaagatgtg tggggaattg gtcgtcagca
     7861 cgccaaacgg cttcggctca tcggtgtgaa gactgcctac gacttcactc agctgcacga
     7921 tgcttgggtg caacatcaca tgtcgattgt cggcctacgc ctcaaacacg atctgcaagg
     7981 ctcgcccacg ctcgatctcg aactggtgca acccaagaaa aatatcgcca ccacacgctc
     8041 cttcgaacgc aattacacca aactcgccga actcgaggaa cgcgtagcca cattcgccgt
     8101 ttcctgcgcc gagaaactcc gcaaacagaa gtcatgctgc aataccatca tggtgtttat
     8161 tcataccaac ggtcaccgca aagatttacc tcagtacagc cggaatatcg tagtcaagct
     8221 acctttcgca accaactccg gaattgaact tgccaaattc gctaagcagg ctcttaagca
     8281 tatctttcgc gaaggttatc actacaaaaa agctggcgta cttgtgatgg acttcacgcc
     8341 acaagacaaa actcaattgc gcctgttcga caatagcaac gcacttcaca ttccgctcat
     8401 gacggcggtc gataagatta acgcgatgtg cggacagcag aagattcggc tcgcagcgca
     8461 agatcaaaaa cgtgtgtgga aaatgaagca ggaaaaacta tcgccccgct acaccaccaa
     8521 actgagcgat atcattatca ttcatacctg atgtgttttc actccaagca atccaaatcg
     8581 gctcaggaac ttaaagctag gtttaaagcc gaatttcttc acgaagaaca gtacacaccg
     8641 ggcatctaca atggatttca atatcctcaa acgcccgtca tcacccatct gcatccccaa
     8701 acaatcgagc tcctgaactg gggacttatt cctgcttggg caaaagacag tagttttcga
     8761 aaaaacaccc tcaatgcccg ttacgaaaca ataggggaga agccttcctt ccgaaacatc
     8821 acccacaacc gatgtttagt acttgctgat ggcttttatg aatggcagtg gttggatgaa
     8881 aaaggcaaaa agaaacagaa atttgaaatc acgactccca atggggaagc atttgccttt
     8941 gctggtttgt ggaccgaatg ggtagataaa tcaaccggtg aaatcctgaa aacatacacc
     9001 atcctcacca ccgaagccaa cgaattaatg agtcagattc ataattcagc gaagcgaatg
     9061 cctatcattg tagatgcacg tcatgagcaa gaatggcttg aaggaaagcc tttaattatg
     9121 ggcaacgctg atttaattgc cgctccaatc tgattcaatt acataaaacg ataaacacag
     9181 tcctcagaat gcgaatcgtt tccgacagat gttttattat tgcaaacaat tacgcacatg
     9241 gaatcgtata ttaatgcctg aaacaacgca tgaacaacag gcagcaactt tttgaagagg
     9301 tcaaacaggt agttttccag ctgttaacgg ataaacttcc tccgcatctg tattatcatt
     9361 gtttggatca taccgaagat gtaattagag aagcgacgcg tttggctaag gctgaacagc
     9421 tcagcgatca agaaacactg cttgtacttt tagctgctgt ttttcacgat accggattta
     9481 ttcaatcacc acaagatcac gaagccagaa gctgcgacat tgctcgggaa atgttaaata
     9541 acttccctct tactaccgat gaactggatt tagtttgtga agctatcatg gcaactaaaa
     9601 tccctcaagt gcccatgagc aaaattgctg aagtactctg cgacgccgat cttgactacc
     9661 tcgggcgtaa cgattacgat gaaatcagcg atcgactatt ccgtgaaata aagcactcga
     9721 atccaaattt cagtctatca gattggatta aacttcagga taatttcttg aaggctcatc
     9781 attattacac agccacaagt cagcgtgagc ggaacgagat taaagctgtt aatcatcagc
     9841 gagttattaa tcaaaataac taggtaattt cctgcgttct tgcatagaaa ttaggcttgg
     9901 tgttctccag tctactactg attctattct cattcaattc ttattcttga gcaatagtta
     9961 atgggcgtca acattcttgt tgaaaatgtt gctctaatgc aacaattcag ttctgattta
    10021 atgctggttt gatgcctaat tggatgaatt aatcaatgta aactcattca atttcttcga
    10081 attttcctat actttcgttc aaaattgact tgtatgcgtt ttcttctgac ttgcatttta
    10141 tgcctgagtt ccctgcttat agttgcacaa ccgcatcctt atggttgtca gcattggcgt
    10201 caatcagcag gaaaaggtca agcattatcc tcctcagcga gcaggagttt aaatgaaagc
    10261 atccttcgca gcgatacctt cgacatcctt cattatgata ttgcaatcga tctaactgca
    10321 tatggaagtc agcaaatttc tgctcaaaca gaaatcacct ttcttcctaa aatggccaat
    10381 ctcgatttca tccgttttga tctcgttcgc ctaattgtcg actccgttaa gaacgaatct
    10441 gggctgcttc cttttgcaac cgatagtcag ttcgtcaaag ttcaatatcc atcagcactc
    10501 acgcttaacg acaccaattc tcttacaatc tattatcatg gtcatcctta tcgagatcca
    10561 gtttggggcg gattctattt tgaaagtggc tatatctaca acttaggaat tggcttatct
    10621 accattcctc ccaattttgg aaaagtttgg tatccctgtt tcgactcatt tgtcgaacga
    10681 gccacatacg cctatcacgt aaaaacatca ggttcgtaca aagctcattg tcagggtact
    10741 tttttgggtg aacagcaatt aagtggtgat acagttattc ggtcatacga attcaatcag
    10801 ccattaccca cacatttatc ggcaattgct gcatctaatt acttcgatct aaacttcacg
    10861 cacaacggta attatgcgcc agtacctgta aaactcaccg ccaaacctgc cgacaccagc
    10921 gcgatgc
//
LOCUS       DAOOQW010000062        10545 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_7859_length_10545_cov_6.221354, whole genome shotgun sequence.
ACCESSION   DAOOQW010000062 DAOOQW010000000
VERSION     DAOOQW010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 10545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10545
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7859_length_10545_cov_6.221354"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1806)
                     /locus_tag="PK281_05380"
     CDS             complement(<1..1806)
                     /locus_tag="PK281_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOY48813.1"
                     /translation="MKKHFLSLILSAAVGSLLAQSAMTPEQLLKVARVSAIGVTKDGK
                     NLVYRASVPDLAANKNSSKLYMIPLAGGVATPLDSLGSLVADASISKDGKYQLQEHLV
                     KVDKVNAVDYYPEMTKANVQIYDGLNYRHWDTWEDGKYRHVFLHKMINGKADEGKDLM
                     PGEPYDSPQQPMGDESDYTFSNDGMQVVYVCKKKSGTAYAISTNTDLYSYDITTGLTS
                     NLTEGRMGYDTDPLFSSQNTLAWLSMKRDGYEADKNDIIVRGKAGDMNLTANWDGTVK
                     SYRWADDGKKIYFTAAIDGTLQLFEVDYPGMTKKLPVVQQLTTGDFDITGIVSQVGNT
                     LLLSRTDMNHASEIYTYDMKSKGLKQLTHVNDELYAGVKMSKVERRYVTTTDGKKMLV
                     WMVYPPDFDPAKKYPTLLYCQGGPQSPVTQFYSFRWNFQVMAAQGYIVVAPNRRGLPG
                     FGVEWNEAISKDWGGQSMRDYLSAIDDASKESYVDKSRLGCIGASYGGYSVFNLAGMH
                     NKRFKTFIAHAGVFDLKSMYGTTEEVFFTNFDMGGAYWEKNNAVAQKTYAEFDPSAKV
                     GNWDTPMLILHGGKDFRVPEGQGFEAFQALQLRNIK"
     gene            complement(1963..3090)
                     /locus_tag="PK281_05385"
     CDS             complement(1963..3090)
                     /locus_tag="PK281_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48814.1"
                     /translation="MKRLTLSLILFVATSFSLLVAQSNRPLLAVGDIDANDALPGNGY
                     STLTEMAMRKMERIGTYEILDQYDMNFLAKRDGINLTGCFSKICLTEMGTKLHVDKIL
                     TGSIRTVGDNITVNFRILDVKNGTVERTHTNDFLKINAEYRNMIEITLDELMGNPVDE
                     EIKKKLTLKNDFDNTVNNPYQLRLRTDGPRMGFTMFTGETAAILGSNRNNGGFEAQPM
                     MFQFGYQFEKQYLNEGNFQALFEFIPMMTGLDQGLFIPSITVMNGLRNNKNGWEFAFG
                     PTFSVVTKSQGYYTADTHKWTLLNDSLQTPENADVITRLDSRGDAAIQAGFIFAFGKT
                     FKSGKLNIPVNAFLIPNNKGLRFGVSFGFNARDRYAYSPVR"
     gene            3305..4303
                     /locus_tag="PK281_05390"
     CDS             3305..4303
                     /locus_tag="PK281_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48815.1"
                     /translation="MPNKTRIFHQQRFIALLKTLVPGQVNLARDLMDILEISQDGAYR
                     RLRCETAITLDETVKICLHYSIPLEALNNEVPDVVTFQFNPLDQSLDQFRNYLATLSE
                     QLKSFRKHAQPDFHYAAEDIPMFYHFGFPALTAFKLLYWMKSILNIPEFESSHYPFPV
                     EEYFDTELLKGLYQHYAEIPGTEIWTDETIESTLQQIKFYWDAGFFVSKDAALTVIDQ
                     MEELIRRVARQTEIGQKIGYNGASTGAVLNVYLCDLMIGNNSIYVSTGESAISSIGYN
                     TFNSILTRNEAFNKQHLLWMDNLKRKSIQISGMAEKIRNQFFKAQLKKIDQLRDAL"
     gene            4505..5827
                     /locus_tag="PK281_05395"
     CDS             4505..5827
                     /locus_tag="PK281_05395"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007646617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOY48816.1"
                     /translation="MSKSSFSDLNLNTPLLNAINDLGFEHPTPIQERAFSAMMAGNDV
                     VGLAQTGTGKTIAYLLPCLRLWKFSKDKLPQTLILVPTRELVVQVVEEITKLATYMNI
                     TAVGVYGGANIRTQKDAIHKGLDILVATPGRLLDLALDGTLKTKTIKRLVIDEVDEML
                     SLGFRPQLERIFDVLPPKRQTLMFSATLSDDVDSMISTYFRAPIRIEAAPAGSPLDNI
                     SQTVIEVPNYNTKLNLLNHLLSQDNMDRVLLFAGSRKLADKLYEHLEAEFPTESGIIH
                     SNKAQNTRFRAVENFAEGKQRILIATDILSRGIDITGVSHVINFDVPDVMENYIHRIG
                     RTGRADAKGDAITFVMEKDAEMIESIEELMQMNIERLPIPEEVEISSVLTLDELPHIN
                     MPGSKVKKSKYEPGPAFHEKKDKNKKVNVRKTRAQQMREKYNKPRRKG"
     gene            complement(5829..6950)
                     /locus_tag="PK281_05400"
     CDS             complement(5829..6950)
                     /locus_tag="PK281_05400"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family N-terminal domain-containing
                     protein"
                     /protein_id="HOY48817.1"
                     /translation="MKKVKFDRAIPYNDLKDLPPSDSIFDTEVLLKWGITSRALAELN
                     RNCLRIPEPNMLINTISLQEAQSSSAIENIFTTDDELYKAISDTVRESNSTHATKEVL
                     RYREAMWAGYLSIKENGKINLKTTIDVFQQIQRTKQGIRSAQSQIVIKRGQSEFKPGE
                     IIYTPPRGEGIIEQKMMNLFTYLNDHKNYTHDPLLKMIIGHYQFEAIHPFVDGNGRTG
                     RILNQLYLINEGLLSHPVLFLSKYILENKDEYYAKLAAVTLRGSWKPWIMYMLDAIHK
                     TAIITNQRIDDILIQMDATYLHAKGKIKWYNLEIHLMIFSQPYIKPKRLGSLLKISSR
                     TTLTKYMNQLVDAGILSPQYDSKEVFFINNDLIRILQSK"
     gene            complement(7064..7948)
                     /locus_tag="PK281_05405"
     CDS             complement(7064..7948)
                     /locus_tag="PK281_05405"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOY48818.1"
                     /translation="MKAVWKAHLAVLIANVIYGANYSVAKEVMPAYFAPFGIIVLRVS
                     GAILLFALLFLRKTERIRKEDRMRFLLCGLMGVACNQLLFFKGLSLTTPIEAAIILTA
                     NPVLVLIAAAVIARERITSWKALGILLGLIGAMILILQRPHTAEGSENYLGNLMILLN
                     AAFYAVYLVIVRPLMQRYRASTVMLFVFLCGWIPILPIGMSEVMAVDWVNVPMWAFGS
                     LAFIVVGATFLAYILNAYALATVSASVVSIYIYLQPFLAALIAIAWGKDELDMSKIIS
                     AAFVFAGVWLTSRPQSKN"
     gene            complement(7968..8900)
                     /locus_tag="PK281_05410"
     CDS             complement(7968..8900)
                     /locus_tag="PK281_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48819.1"
                     /translation="MINSRIRPFSIIVFFLILVGKPAISCDVCGCSASGLSGGMFPQI
                     QSNMLGWRFTPARFTHPATLDNMNGASLVKEDRYTDQELFFRWYAHKRLQLWITAPYR
                     VHERIETLRTTTIQGIGDMQASALYTIVNTNQDTIHRNWKHIWLAGAGVSLPTGKYRQ
                     RDETLTTLPNGFQLGTGAYSAVLNSVYLIRYKRLGVQVQGDYRTWTANESQFQKGDQY
                     SVQSQVFYRFPVKSLVLLPQAGMRYEHLDQDIEFDAPRVQSGSTSSWATAGLDIYAKH
                     WMLGCSVQSPIQQTLTSAQPSSGVRFSVMAGIVW"
     gene            complement(8956..9744)
                     /locus_tag="PK281_05415"
     CDS             complement(8956..9744)
                     /locus_tag="PK281_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48820.1"
                     /translation="MKRSLNKILFFLSIATISLSSCKKDEETPTPTPTPTPQTGMFKV
                     EFEHGFDGAEFAFDTTYTNAAGESLTFTKFDYYISNVKLTKTDGTTWSQPNSYYLLKS
                     NDDLSRLLTISDVPTGTYSGITFSIGVDSTHNVSGAQTGALDPANEMFWSWSTGYIFI
                     KAEGTSPASSMGGAFEYHIGGFQTANNTNCVRTVSHTFGGATMTIAPNTAPQIHMAVD
                     IAKLFNGTSNLSVAATPMVHMPGADAVAIADRFQEAFEFEHLHE"
     gene            complement(9831..10199)
                     /locus_tag="PK281_05420"
     CDS             complement(9831..10199)
                     /locus_tag="PK281_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48821.1"
                     /translation="MRTLAVLFVFLILLPSLVKVAVVGNYVVQYERYATELCENQDKP
                     ELACNGKCQMMKELNEAEQNAPVAPSLPEFKVKEQPVCELNSAALISFSVELKQEQPL
                     SNFEGLPEGIFGNVFHPPCC"
BASE COUNT         3195 a         2411 c         1901 g         3038 t
ORIGIN      
        1 tttgatattg cgtaactgca aagcttggaa cgcttcaaat ccttgtcctt caggaacacg
       61 gaaatctttt cctccgtgaa gaatcaacat cggtgtatcc cagttaccca ctttcgcact
      121 tgggtcaaat tcagcatagg ttttttgtgc cactgcattg ttcttttccc aataagcacc
      181 acccatatca aagttggtaa agaacacttc ttcggttgtt ccatacatac tcttcaaatc
      241 gaaaacaccc gcgtgagcaa taaaggtctt gaaacgcttg ttgtgcatac ctgccaaatt
      301 gaaaacggaa taaccaccat agctagctcc aatacaacca agtcgcgatt tgtccacata
      361 actttctttc gaagcatcat caatcgcact caaataatcg cgcatcgatt gtccacccca
      421 gtctttacta atcgcttcat tccactccac tccaaaacct ggtaatccac gacggttagg
      481 tgcaactaca atatatcctt gcgctgccat tacctgaaaa ttccaacgga atgagtaaaa
      541 ctgagtcact ggagattgcg gtcctccttg acaatacaac aaggttggat atttcttagc
      601 tggatcaaaa tcgggtggat aaaccatcca caccaacatc ttcttaccat cggttgtagt
      661 cacatagcga cgttcaactt tactcatttt caccccagca tacaattcat cattcacatg
      721 agtaagctgc tttaagcctt tgctcttcat gtcgtaagta taaatctctg aagcatgatt
      781 catatccgtg cgactcaaca acaaggtgtt tccaacttga gaaacaattc cagtaatatc
      841 aaaatcacca gtggtcaatt gctgaacaac tggcaatttc ttagtcattc caggataatc
      901 cacttcaaac aattgcaagg ttccatcaat cgctgcagtg aaatagattt tttttccatc
      961 atctgcccaa cgatacgatt ttactgtacc atcccaattc gcagttaaat tcatatcgcc
     1021 cgcttttcca cgaacaataa tgtcgttctt atccgcttca tagccatcac gtttcatgct
     1081 taaccaagcc aaggtatttt gagaggagaa caatggatcg gtatcataac ccattcgccc
     1141 ttcagtcaaa tttgacgtta aaccagtagt tatatcatag ctgtacaaat cggtattggt
     1201 gctgattgcg taagctgttc ctgatttttt cttgcacaca taaaccactt gcattccatc
     1261 gttgctgaat gtgtaatcgc tttcgtcgcc catcggttgc tgcggactat catacggctc
     1321 acccggcatc aagtcttttc cttcatccgc ttttccattg atcattttgt gaagaaaaac
     1381 gtgacggtat tttccatctt cccacgtatc ccagtgacga taattcaagc catcgtaaat
     1441 ctgcacattg gctttggtca tttcgggata ataatcgaca gcgttcacct tatccacttt
     1501 caccaaatgc tcttgaagtt ggtattttcc atctttacta atgctagcat cagccactag
     1561 cgaacccaaa ctatcgagtg gagttgcaac tcctccggct aatggaatca tataaagttt
     1621 agagctgttt ttattggcag ctaaatcagg aacgctagct cgatacacga gattcttacc
     1681 gtcttttgtc actccaatag ccgatacgcg tgctactttt aataactgct ctggagtcat
     1741 ggccgattgt gccaaaaggc taccaacagc ggcactcaag atcaaggaaa ggaaatgctt
     1801 tttcatttcg gaattcagtt tagggcggtg aatatacagg attcgttcga ttcaatttgc
     1861 tttcacagat gttcaatact cgttcaatct cacccaaata aaaaacgccc gaagatttct
     1921 ctccgagcgt ttgccccaat catgaggact agctgctgac acttatctta ccggactata
     1981 tgcataacga tcacgtgcat tgaatccgaa cgacactccg aaacgcaaac ctttgttgtt
     2041 gggaatcaag aaggcattca caggtatatt caattttcca gatttgaacg ttttaccaaa
     2101 agcgaagatg aaacctgctt gtatggctgc atcaccgcga ctgtccaaac gagtaatcac
     2161 atcggcattt tctggagttt gaagactatc gtttaacaaa gtccatttat gtgtgtcggc
     2221 ggtgtaataa ccttgacttt tagttactac tgagaaagtt ggtccgaatg cgaattccca
     2281 tccgtttttg ttgttacgta aaccattcat cacggtaata cttggaatga aaagtccttg
     2341 atctaatcca gtcatcatcg gaataaactc aaacaaggct tggaaattcc cttcattcaa
     2401 atactgtttc tcgaactgat atccgaattg gaacatcatc ggttgtgctt caaaaccacc
     2461 attgttgcgg ttcgaaccta aaattgcagc tgtttctccg gtgaacatag tgaatcccat
     2521 acgcggacca tcagttctca aacgcagttg atatgggttg tttacggtat tatcgaaatc
     2581 atttttcagg gtgagttttt tcttgatttc ctcatctacc ggattaccca tcaactcatc
     2641 caaagtaatc tcaatcatat tgcggtattc agcattgatt ttcaagaaat cgttagtgtg
     2701 tgtacgttct accgttccgt ttttcacatc gagtatgcgg aaattcacgg tgatgttatc
     2761 gcctactgta cgaatgcttc cggttagaat tttatctaca tgaagttttg ttcccatttc
     2821 ggttagacaa attttcgaaa agcaaccagt caaattaatt ccatcgcgtt tagctaagaa
     2881 attcatgtcg tattgatcaa gtatctcgta agtaccaatg cgctccattt tacgcatggc
     2941 catttcggtt aaagtggaat aaccgtttcc aggaagtgca tcatttgcat caatatctcc
     3001 caccgctaaa agtggacgat tgctttgagc tactaaaagt gaaaatgaag tagctacgaa
     3061 aaggattagg gaaagtgtca gacgtttcat aagaatttgt ttttggtttt ttgttgagac
     3121 aaagttgcag cagttcaaca cctcacttct acggatattc accaacacat taggacttca
     3181 attaatattt aataattcca cgccactaac agatctaatt ttgcacttga tttagtatta
     3241 ttttcaaact acatttgaca tttacgcata tcacacaaat caaaagcaac tcatttacgg
     3301 aataatgcca aacaaaaccc gcatttttca tcagcaacgt tttattgcgc tgctcaagac
     3361 attggttccc ggtcaagtaa acctggcgcg cgaccttatg gatattctgg aaatcagtca
     3421 ggacggggcg taccgaagat tgcgttgcga aacagccatc acgctcgacg aaaccgtgaa
     3481 gatttgcctg cattacagca ttccgctcga agctctcaac aacgaagtgc ccgacgtagt
     3541 cacctttcag ttcaatccgc tcgaccaaag cctcgatcag ttccgcaatt acttggcaac
     3601 gctgagcgaa caattgaaat ccttccgcaa acacgcacaa cccgattttc actacgccgc
     3661 cgaagacatt cccatgtttt accatttcgg ctttcctgct ttgaccgcct tcaagctgct
     3721 ttactggatg aaatccattc tcaatattcc cgagttcgaa agcagtcatt acccttttcc
     3781 cgtagaagag tatttcgata ccgaacttct aaaaggactg tatcagcact atgccgaaat
     3841 tccaggaacc gaaatctgga ccgacgaaac catcgaaagc accttgcaac aaatcaaatt
     3901 ctattgggat gcgggattct ttgtcagcaa ggatgcggca ttgactgtaa tcgaccaaat
     3961 ggaagagctc attcgtcgcg ttgcccgcca aaccgaaatc ggacaaaaaa ttggctacaa
     4021 tggcgcatcc acgggcgctg tgctcaatgt gtacctctgc gatttaatga ttgggaacaa
     4081 ctccatctat gtaagcaccg gcgaaagtgc catttcctct atcggttaca acaccttcaa
     4141 ctccatcctc acccgtaacg aagccttcaa caaacaacac ttgctctgga tggataacct
     4201 aaaacgtaaa tccattcaaa tcagcggcat ggctgagaaa atccgcaatc aattcttcaa
     4261 agcccaattg aaaaaaatcg accaactccg cgacgcgctt taaaaaaata agtgtttggg
     4321 cgtatgcgta tgtctatttc gcaccgctac atatacacac gcaccgcgct atcgcccatg
     4381 cacggcattt ggctctatcg cttacgcaga cattccattt catatgttgc taaatactac
     4441 agcctatgga gaatagtcaa acaagcttat cttttcacct acctttacca gcccagcaaa
     4501 caccatgagt aaaagcagtt tttccgattt aaacctcaac actccgctcc taaatgctat
     4561 caacgattta ggttttgagc atccaactcc cattcaagaa cgcgccttct cagctatgat
     4621 ggcgggtaac gacgtggttg gattagcaca aactggtacc ggaaaaacta tagcttatct
     4681 cctgccctgc ttgcgtctct ggaaattctc caaagacaaa cttcctcaaa cacttatcct
     4741 cgttccaacc cgcgaactag tagttcaagt agtcgaagaa attaccaaac tcgctaccta
     4801 catgaacatc actgctgttg gtgtgtatgg tggtgcaaac attcgtaccc aaaaagatgc
     4861 gattcacaaa ggcctcgaca ttctcgttgc cactcccggt cgcttgcttg atcttgctct
     4921 tgatggaaca ttaaaaacca aaaccatcaa acgtttggtc atcgacgaag tagatgaaat
     4981 gctcagcctc ggttttcgtc ctcagctcga acgcattttc gatgtgcttc ctccaaagcg
     5041 ccaaacgctc atgttctctg ccacgctttc cgacgatgtc gattccatga tcagcaccta
     5101 tttccgtgct cctatacgaa ttgaagctgc gccagctggt agtccgctcg ataacatctc
     5161 gcagactgtt attgaagtgc ctaactacaa caccaaactc aatctgctca atcatttgct
     5221 cagtcaagac aatatggacc gcgtgctcct atttgccggc agcagaaaat tagctgataa
     5281 actttacgag cacctcgaag cagaattccc aaccgaatcc ggcatcatcc actccaataa
     5341 agcgcaaaac acccgttttc gagccgtaga gaattttgcc gaaggaaaac aacgcatcct
     5401 aattgccacc gatattctat ctcgcggtat tgatattact ggcgtttcgc acgtaatcaa
     5461 tttcgatgtg cccgatgtga tggagaatta catccaccgc attggccgta ccggtcgtgc
     5521 tgatgcaaaa ggcgacgcca ttacgtttgt gatggagaaa gatgccgaaa tgattgaatc
     5581 cattgaggaa ttgatgcaaa tgaacatcga gcgtttgcct attcccgaag aagtagaaat
     5641 atcaagcgtc ctcacactcg acgaactacc gcatatcaat atgccgggtt ctaaagtcaa
     5701 gaagagtaaa tacgaacctg gaccagcttt ccacgaaaag aaagacaaga ataaaaaagt
     5761 caacgttcgt aaaacccgcg cccaacaaat gcgggagaaa tacaataagc caagaagaaa
     5821 aggctaattt atttagattg aagaattcta attaggtcgt tgtttataaa gaacacttct
     5881 tttgaatcat attgtggact taaaattccc gcatcaacta attgattcat atatttcgtt
     5941 aaagtagttc gactactaat ttttaataaa cttcccaagc gtttgggttt tatatagggt
     6001 tgagaaaata tcatgagatg aatctccaaa ttataccatt ttattttgcc ttttgcatgt
     6061 aaataagtag catccatttg aattaatata tcatcaattc gttggttagt gataatggca
     6121 gttttatgaa ttgcatctag catatacatt atccaaggct tccaagagcc tcgcaatgta
     6181 acagcagcaa gcttagcata atattcatct ttattctcta aaatatactt actcaaaaac
     6241 aaaacagggt gagataatag accttcattt attaaataca attgatttaa aattcggcct
     6301 gttctaccat tcccatctac aaaaggatgt attgcctcaa attgataatg accaattatc
     6361 atctttaaaa gtggatcatg agtatagttt ttatgatcat ttaaataggt aaatagattc
     6421 atcatctttt gttcaataat gccttccccc cttggcggtg tataaattat ttctccaggt
     6481 ttaaattcac tctgtcctct tttaattacg atttgtgatt gtgctgaacg aattccttgt
     6541 ttagttcttt gaatttgttg aaaaacatcg atcgtagttt ttaaatttat ttttccgttt
     6601 tctttaatag ataaatatcc agcccacatt gcttctcgat atctaagtac ttcttttgtt
     6661 gcatgagttg aattcgattc tcgaacagta tcagatattg ctttgtataa ttcatcatcg
     6721 gttgtaaaaa tattttcaat tgcactagag gattgagctt cttgaagtga aattgtatta
     6781 attaacatat tcggttcagg tattcgcaaa caattccgat tgagttccgc caatgctcgc
     6841 gatgtaatcc cccattttaa taaaacctca gtatcaaaaa ttgagtctga aggaggtaag
     6901 tctttaagat cattatatgg aatagcccta tcaaatttga cctttttcat atgtccaata
     6961 aattagctaa atgtgaactt aagtcaaaaa tacattcaaa aatggacaga attacacacg
     7021 tgttcatttt tcagctattt tatgaacacg tagacctaag ttcctaattt ttactttgcg
     7081 gtcggctcgt taaccaaaca cctgcaaaaa caaaggcagc agagataatt ttggacatat
     7141 ccagctcgtc ttttccccaa gcaattgcaa tgagtgcggc taaaaagggc tgcaaataaa
     7201 tgtaaatact caccaccgaa gcactcacag tagccaatgc ataagcatta agtatatagg
     7261 ctagaaacgt tgccccaact acaataaacg ctaaagaacc gaatgcccac atgggaacat
     7321 tcacccaatc aaccgccatt acttcgctca tcccaatggg tagaatagga atccatccac
     7381 ataagaacac aaataacatc accgtactcg ctcggtagcg ctgcattaac gggcgaacaa
     7441 tcaccagata aactgcataa aaagcagcat tcagtaaaat catcaagtta ccaagataat
     7501 tctcacttcc ttctgccgtg tgagggcgct gaagtataag aatcatcgca ccaattaatc
     7561 caagcaaaat ccctaaagct ttccagctag ttatacgttc tcgtgcaatc acagctgcgg
     7621 caatcaatac caaaactgga ttagccgtta aaataatagc ggcttcaatg ggtgtagtta
     7681 agctgagtcc tttgaaaaat aagagttgat tacaggcaac gcccatcaat ccacataaaa
     7741 gaaaacgcat ccgatcttct ttccgaatgc gttctgtttt tctaagaaag agtaatgcaa
     7801 aaagtaaaat tgcccctgat acgcgcagta cgattattcc aaacggtgcg aaatacgccg
     7861 gcatcacctc tttggcgacc gaataattgg ctccgtatat cacattcgca atcaatactg
     7921 ccaaatgcgc tttccacact gctttcatgc agcgcgaagg tacatcatca ccaaacgatt
     7981 ccagccatca cactaaatcg tactcccgaa ctcggttgcg ccgaagtaag cgtttgctga
     8041 ataggcgatt gcaccgaaca gcctaacatc caatgtttag cataaatatc taaacctgcc
     8101 gttgcccacg aacttgtact tcccgattgg acacgtggcg catcaaattc aatatcttga
     8161 tctaaatgct cataccgcat tcccgcttgg ggcaataaca ccaaactttt cacaggaaat
     8221 cggtaaaaca cttgcgattg cacactgtat tgatcaccct tttgaaactg tgattcatta
     8281 gccgtccagg tgcgataatc gccttgaact tgcacaccga gacgcttata acgtatcaaa
     8341 tacaccgaat tcaacacagc agaatacgct cctgtaccta attgaaaacc attaggtaaa
     8401 gtggtcaatg tttcatcgcg ctgacgatat tttccggttg gcaaagaaac acctgcacca
     8461 gctagccaaa tgtgtttcca atttcgatga atcgtgtctt gattggtatt cacaatcgtg
     8521 tacaaagcag atgcttgcat atctccaatg ccttgaatgg tagtagttcg caatgtttca
     8581 attcgttcgt gaacacgata cggtgctgtt atccataatt gaagtcgttt gtgagcatac
     8641 caccgaaaaa acaattcttg atcagtatag cgatcttcct ttaccaagct ggcaccattc
     8701 atattgtcaa gtgtagctgg atgggtaaat cgtgcaggag taaaacgcca accaagcata
     8761 ttactctgaa tttgtggaaa cattcctccc gacaaaccac tagccgaaca tccacataca
     8821 tcacagctaa tagccggttt ccctactaga atcaagaaaa aaacgataat cgaaaatgga
     8881 cgaatccttg agtttatcat acgcgttaaa tttaaacagg cgcataatgt gagattacgc
     8941 gcctgtactg tatgattatt cgtgtaaatg ctcgaattcg aatgcctctt ggaaacggtc
     9001 agcaatggca actgcatcag cccctggcat gtgaaccatt ggagttgctg caacacttaa
     9061 attgctagtg ccattaaaca atttagctat gtcaacagcc atatgtattt gtggagctgt
     9121 attcggagca attgtcattg tagcacctcc aaaagtatgg cttactgttc tcacacaatt
     9181 ggtattattt gctgtttgga aacctccaat atggtattca aaagcacctc ccatcgaaga
     9241 ggctggacta gttccttctg ctttgatgaa gatataacca gtactccagc tccagaacat
     9301 ttcatttgct ggatccaatg ctccagtttg tgctccactc acattatgag tagaatcaac
     9361 tcctatagaa aatgtaattc cagaataggt tcctgttggc acatccgaaa tagtaagtaa
     9421 acgactcaaa tcatcattac tctttaataa gtagtaacta ttcggttgag accaagttgt
     9481 accatccgtt ttcgtcagct tgacattaga aatatagtaa tcgaacttgg tgaaagtcaa
     9541 tgattcacca gctgcattgg tataagttgt atcaaaagca aactctgccc catcaaaacc
     9601 atgctcaaac tccactttga acattcctgt ttgaggagtt ggggtgggtg ttggcgtagg
     9661 tgtttcttcg tcttttttac aagaagaaag tgaaatggta gcaatagaaa gaaagaaaag
     9721 gattttgttc aatgaacgtt tcataggtat aaaaatgtca cagccaattc attcagagaa
     9781 tgagctgcag attagaaaat gtgtttaaaa aagaatcaac tcaattgctt ttagcaacaa
     9841 ggtggatgaa acacatttcc gaatatacct tcgggtaatc cttcaaaatt tgaaagcggt
     9901 tgttcttgct tcaattcaac agaaaacgaa ataagtgctg ctgaattcaa ttcacaaaca
     9961 ggctgttctt taaccttaaa ctcgggtaat gatggagcaa ctggagcgtt ttgttctgct
    10021 tcgttcagct ctttcatcat ttgacatttc ccgttacaag ctaattcagg cttgtcttgg
    10081 ttttcacaca actctgtagc atagcgttca tattgcacca cataattccc aactaccgcc
    10141 acttttacca aagaaggcag aagaataaga aatacaaata aaacagctaa agtccgcatg
    10201 attctggact gcaaaaataa aggaatattg agcttataaa atgatttatt tcatataata
    10261 gttaacccgc gttgtaccat taaaaattaa taatgttctt ccatgaaatt gtaacgaata
    10321 agccaatatt caatgctatt ggatggtttt aaataaattt attttaaact aatattagat
    10381 ttagaaacgg atcagagcgt tggatatgtc tagggggcga gggtgtgttt cgacatagtt
    10441 gagaataaag cttgtcctaa caaaatggtc gaaaaaaggc catgagcgca tgcagcatcg
    10501 tctagacttg accttcttta ctcgtaaatg tgtatttttc aaagg
//
LOCUS       DAOOQW010000063        10515 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_7885_length_10515_cov_4.675526, whole genome shotgun sequence.
ACCESSION   DAOOQW010000063 DAOOQW010000000
VERSION     DAOOQW010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 10515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10515
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7885_length_10515_cov_4.675526"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /locus_tag="PK281_05425"
     CDS             <1..466
                     /locus_tag="PK281_05425"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOY48822.1"
                     /translation="AAKVIEHSPIPVIVIPDSFRNKAIKNIGYASDLAHVSDELTKIL
                     PIAEALGSKVEIFHVEPTFPTSEAFKKFDAEKNMAEWKKKFKLDSLTYKMVRTKFDND
                     FYTGVDKYRRNSKPDMICMVTHRRNWVSKILDPSKSKGIAYHNEIPVLCIKG"
     gene            531..995
                     /locus_tag="PK281_05430"
     CDS             531..995
                     /locus_tag="PK281_05430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48823.1"
                     /translation="MFGNKYLIPVVSGVCFCIALISCNHAKQLEENISSHGKSESHNQ
                     GLNCMDCHKKGGEGEGWFYIAGTAYHANEQSPAKDVTVLMYTQPDGQGSPKYTIEGDQ
                     LGNFYTTAITGFGAGLYPAIIFNSDTSYMASSITNGACNSCHGVSTTRITVD"
     gene            complement(996..1334)
                     /locus_tag="PK281_05435"
     CDS             complement(996..1334)
                     /locus_tag="PK281_05435"
                     /inference="COORDINATES: protein motif:HMM:NF025494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4296 domain-containing protein"
                     /protein_id="HOY48824.1"
                     /translation="MRNVLFASVLFLLSACQSDNEVPEGILPDSTMKSVLVELSIVDA
                     AYNVSMTNMNAPKFKSELFYEQVMKQHGTTRAQFIRSMDFYAQHTKRLQKIYEDALVD
                     LANRQAKANQ"
     gene            complement(1331..2683)
                     /locus_tag="PK281_05440"
     CDS             complement(1331..2683)
                     /locus_tag="PK281_05440"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004569327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HOY48825.1"
                     /translation="MGKGYFIHNATIINEDKRFVGCLKITGSFIESIYEGPIKPEENL
                     KNHQVIDAQEYWLIPGAIDDQVHFREPGLTWKGDLYTEPKAAVAGGVTSFMEMPNTVP
                     GAVTQALLADKYVIAAEKSLTNYSFYLGTTNSNLDQLLKTDPKTVCGIKIFMGSSTGD
                     MLVDNQHTLEDIFREVKMLIALHCEDDNLIKANAQLAREQFGDQVPFTLHSTIRNAEA
                     CYSSSSKAVELASRFDTRLHILHISTEKELSLFRNDIPSSQKRITTEACVHHLWFEEA
                     DYQTKGAWIKWNPAIKSHRDREAIREAVINGKIDVLATDHAPHSIEEKKKSYFNCPSG
                     GPLIQHALPALFELCRQGVFTPELVVRKYAHTVADLFQVHKRGYLREGYYADVVLINP
                     NQSQLVTEESLLYKCGWSPFTGTRFNSVVSRTFVNGNLVYDDGVFDETNKGMRLSFDR
                     "
     gene            complement(2701..3957)
                     /locus_tag="PK281_05445"
     CDS             complement(2701..3957)
                     /locus_tag="PK281_05445"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015753000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOY48826.1"
                     /translation="MKVFKFGGASVKDANGVRNVASIISKYTDEQLVIVVSAMGKTTN
                     ALEKVCQAFFHQTGEAPALLEEVKATHQRIIDDLFPNKNANLQQEFDSIIAELYWAIE
                     ETQIKSYDYEYDQIVSQGEFLSTRIVAAFLNDQNIKTQWLDARDLIHTDNTYREGMVD
                     WDKTKHAVSQIIQPTKNSLLYLTQGFIGGTSENFTTTLGREGSDYSAAILAYCLDAES
                     VTIWKDVPGVLNADPREFPQAQLLPELSYYDAIELAYYGATVIHPKTIKPLQNKNISL
                     FVKSFVDSTAAGTRIREDNLRQEVPTYIFKKDQVLISVSPRDFSFILEENLRDLFDLF
                     TKFRAHVNVMQNSALSFSVCVSIEANRLQSLLKELELKYKVKYNEGCELITIRNYTDE
                     LAQKLIGSRPVLMEQKSRHTLQIVLR"
     gene            complement(3954..4439)
                     /locus_tag="PK281_05450"
     CDS             complement(3954..4439)
                     /locus_tag="PK281_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019975808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOY48827.1"
                     /translation="MNYIIEEATPDDVPHILALIKQLAEYERASNEVTVTESILLADG
                     FGPNKIYSCFVARMEDKSIRGIALYYNKYSTWKGSCIFLEDIIVEEPFRGYGIGKELF
                     RAVYQIARNSKAGRMEWQVLDWNEPAIGFYKRFNAQFDAEWINCKFNYEQLQQTAAED
                     L"
     gene            complement(4567..5532)
                     /locus_tag="PK281_05455"
     CDS             complement(4567..5532)
                     /locus_tag="PK281_05455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48828.1"
                     /translation="MAKNIEFSAEEMSELHAFYIQEMEKAEERLKAIKSIIAKIGTAP
                     KKRGRKPSAEKQLTKSISADLKAAAPKVAGRKRGRPAKPKTDAPKAAPKRRGRPAKVT
                     ASPVVASSTAPVVATEPKKRGRKPGSKASAKPAVAKKASTKVAKAKVVKAAKVSAPKA
                     AKKAVKPVAKKAAKPVVKKEAAVKAPKAPKVKAAKAPKVKAAKAPKVKAAKAPKVKAE
                     KAPKVKAVKAPKVKAVKAPKVKAVKTVDAPKPVVKADVKSDKPSTPVAPKAVKIAKKP
                     APAKKPVAVKVKPAKKVEPKKVEVKKAPEASAPAATPTSNPTTQA"
     gene            complement(5900..7072)
                     /locus_tag="PK281_05460"
     CDS             complement(5900..7072)
                     /locus_tag="PK281_05460"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HOY48829.1"
                     /translation="MSVYRQIIFLCFLTRALIAQEEQVRIGLFRASEITFVQFEMGEN
                     TWLEIDPGVRIWGEESGAKIQIQVKGNSLEIRTSSLVLGTANKVRFHSNSGNALFLKP
                     LKPDYKGNRYAGDIVVSVSSGKLKFINQVNETDYLVGVLRGEVGYNKPVSLYQVHAIL
                     SRTYARYYQWRHKSEGFELCDQTHCQVYKGYFDYDPYRLAVLTTRNIVVKDSLTGNLA
                     ELLFHSNCGGITNASEDVWKSTLSYCRSTEDTFCLVGKNAFWNTRITLEKFCEKLAIA
                     YPTDSISKLELCTSICSFEENRPHQIQLANRTFRMIDLRTRFDLKSAWFDWECRNDSV
                     YFYGKGFGHGVGLCQEGAIRMSELGFGPEAILRHYFTGILITGTPLKPEDIEQTKF"
     gene            complement(7146..8258)
                     /gene="lpxB"
                     /locus_tag="PK281_05465"
     CDS             complement(7146..8258)
                     /gene="lpxB"
                     /locus_tag="PK281_05465"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HOY48830.1"
                     /translation="MKYYVIAGEASGDLHGSNLLKAIKEEDEQAEFRCWGGDLMQSQG
                     ATIVKHYRDLAFMGFTEVLLNIGTIVKNIRFCKADILAFNPDVLILIDYPGFNLRIAE
                     FAHENSIRVFYYISPQIWAWKQNRVFKIRKVVERMYVILPFEKDFYARFNVDVTFVGH
                     PLLDAIQTFVPDSEFRQRHGITNQPIIALLPGSRKQEISKMLPEMLQSIKSLDTCIPI
                     IAGAPSIDPAQYQEWSGGTQVKVISGETYQLLSHSQAALVTSGTATLETALFSVPEVV
                     CYKGGQLSYWLARRLIKVKYISLVNLILDRMAVPELIQDDMNARKIEQTLSTLIQEGK
                     ERTAQLQDFSELKIKLGGPGASKRAAQDMVMRLMKS"
     gene            8329..9093
                     /locus_tag="PK281_05470"
     CDS             8329..9093
                     /locus_tag="PK281_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008239822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOY48831.1"
                     /translation="MKVTFLGTGTSQGVPVIACTCEVCASTDLRDNRLRCSILVETEA
                     GSFIIDTGPDFRQQMLRQNVKRLDAVLYTHEHKDHIAGLDDIRAFNFVLQKHMDVYLS
                     NYVEDALRREFAYIFAEDKYPGIPLINLHRIDENPFVLLGKTIIPIMVSHYKMPVFGY
                     RIGDFCYVTDAKTISASELDKMKGCKVIVLNALRKETHVSHLTLDEAIEILEYLKPEQ
                     AYLTHISHQLGKHQDIEPELPSFIRLAYDGLTIECS"
     gene            complement(9102..>10515)
                     /locus_tag="PK281_05475"
     CDS             complement(9102..>10515)
                     /locus_tag="PK281_05475"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOY48832.1"
                     /translation="QNQCGYQLDMSISSAGIIDLTPPTLQAVTSQVSCGSTAITFSFS
                     EFVKCNTVNVTGFSLTDPLGVDIPLTGITSIACLNGGLYDKEFTLLLGQQLVQGGSYE
                     IQSFGRVEDNCGNFDPDTQSIFFTFNAVEIQTVNQTQLVDCRANNGIADVTTQFGTGP
                     IAYAWSPSGQTGQTAQGLGFGWQYVSVIDALGCIDRDSIFIEDINNFQVQVVILPDTC
                     SFGLGSAFVLATGGTPFEDRPGIQQEYNYFWNVTDQQNDTTFVDSLLTGSYELSVTDS
                     FGCQYSTIVNIPDYRFNLIPDFVFSPDTTPIPGIFPTVSFVNQSINATNFLWNFGSGE
                     ISTEYEPDYVFPGSGTYDVELIAMNSFGCKDSISKQVTIDFLYTFFAPNAFTPNDDLV
                     NDSFNVVVTGIMDSTYLMVIFDRWGKEIYVTNDKKQAWSGKDSSSGKMYASGCYMFRS
                     SFIDQSGKKHVVYGKVLLIG"
BASE COUNT         3256 a         2015 c         1840 g         3404 t
ORIGIN      
        1 agcagctaaa gtgattgagc attcgcctat tcctgtaatt gttattccag atagtttccg
       61 aaataaggct attaagaata tcggttatgc aagcgattta gctcatgtaa gtgatgaatt
      121 aactaaaatt ttacctattg cagaggcgct tggttcgaaa gtggaaattt tccatgttga
      181 gccaacattc ccaactagtg aagcattcaa gaaatttgat gcggaaaaga atatggctga
      241 atggaaaaag aaattcaaac tagattctct cacttataaa atggttcgaa ctaaattcga
      301 caatgatttt tatacaggag tggataaata tcgtcgcaat tcaaaacccg atatgatttg
      361 tatggttact catcgtcgta attgggtttc taaaattctc gatccaagca aaagtaaagg
      421 cattgcctac cataacgaaa taccagttct ttgcataaaa ggctaaacca aagcttgtat
      481 aacatacgtt aaacaagttg atatcgttta agcgattaaa taagcgtttt atgttcggaa
      541 ataagtactt gattcctgtt gtttcaggtg tctgtttttg tattgcattg atttcttgta
      601 atcatgctaa acaattagaa gaaaatatca gctctcacgg taaaagtgag agccataatc
      661 aaggcttgaa ttgcatggat tgccacaaaa aaggcggtga aggcgaaggt tggttttaca
      721 ttgctggcac agcttatcac gcaaatgagc aatcaccagc taaagacgta acagtcttaa
      781 tgtatactca accagatggt cagggttctc ctaaatatac gattgaagga gatcaactag
      841 ggaattttta caccactgcc ataactggat ttggagcggg tttgtatcct gcaattattt
      901 tcaattccga cacatcgtac atggcatctt ccattacaaa tggagcatgt aattcctgtc
      961 acggtgtgag tacaactcga ataactgttg attaattact gattggcttt agcctgacga
     1021 ttggctaaat caactaacgc atcttcatat atcttttgca aacgcttcgt gtgttgcgca
     1081 taaaaatcca tactgcgaat aaattgagca cgcgtggttc cgtgttgttt catcacttgc
     1141 tcataaaata attccgattt gaatttagga gcattcatgt tcgtcatact cacattataa
     1201 gcagcatcca caatactcaa ttcaactaaa accgatttca tcgtactatc aggcaaaatg
     1261 ccttctggta cttcattatc cgattgacag gcagataata aaaataaaac acttgcaaat
     1321 aggacgtttc tcatcgatca aaacttaggc gcattccttt gttcgtttca tcaaaaacgc
     1381 cgtcatcgta aaccaaattt ccatttacaa acgtacgtga aacaaccgaa ttaaatcgag
     1441 ttccagtaaa gggagaccat ccgcatttat acaaaagtga ttcttctgta accaattgcg
     1501 attgattcgg gttaattaaa accacatctg cataataccc ctcgcgtaaa tatccgcgct
     1561 tgtgaacttg aaataaatca gctaccgtgt gagcatattt ccgaacaact aattcgggag
     1621 taaaaacacc ttgtcgacac aattcaaaca aagcaggtaa tgcatgttga attaatggtc
     1681 cgcccgaagg acaattgaaa tacgactttt tcttttcttc aatcgaatga ggagcatgat
     1741 cagtagctaa aacatcaatt ttaccattga ttacagcttc acgaatagct tcacggtcac
     1801 gatgactttt aattgcagga ttccacttta tccaagcacc ttttgtctga taatcggctt
     1861 cttcaaacca taaatgatgc acacaggctt ctgtagttat tcttttttgt gatgatggaa
     1921 tatcgtttct gaataacgaa agttcttttt cagtagagat atgtaaaata tgtaaacgtg
     1981 tatcaaaacg gctagctaat tcaactgctt tagatgatga actataacac gcttctgcat
     2041 ttctaattgt ggaatgtagc gtaaatggaa cttgatcgcc aaattgctct cgtgctaact
     2101 gagcattggc tttaatcaaa ttatcatcct cacaatgtag ggcgataagc attttcactt
     2161 cccgaaaaat atcttcgagt gtatgctgat tatctacgag catatcccca gtactcgaac
     2221 ccatgaatat cttaattcca cataccgttt tgggatctgt cttgagtaat tgatctaaat
     2281 tggagttagt tgtacccaga taaaaactat aattcgtgag cgatttctct gcagcaatta
     2341 catatttatc ggcaagtaaa gcttgagtaa cagctcctgg gacagtatta ggcatctcca
     2401 taaagctagt tacaccaccg gcaacagcag cttttggctc ggtatataaa tctcctttcc
     2461 aagttagtcc aggctcacga aaatgcacct gatcgtcaat cgctccgggt attaaccaat
     2521 attcttgtgc atcaattacc tgatgattct tcaggttttc ttcgggtttg attggacctt
     2581 cataaatgga ttcgataaaa gaacctgtaa ttttcaaaca tccaacaaaa cgcttgtctt
     2641 cgttgatgat ggttgcatta tgaataaaat atccctttcc catgacgatt tgtttcggtc
     2701 ttagcgtaaa acaatttgca aggtatgcct acttttttgt tccatcaaaa caggtcgaga
     2761 acctataagt ttttgagcca attcatcggt ataatttcgg atggtaatta attcgcagcc
     2821 ttcattatac ttcactttgt atttcaattc caattctttt aacaaggatt gcaaacgatt
     2881 tgcttcaata ctcacacaaa cagaaaaact aagtgccgaa ttttgcatca cattcacatg
     2941 tgctctgaat ttggtgaata ggtcaaataa atcacgcaaa ttttcttcta aaatgaagga
     3001 gaaatcacgc ggtgaaaccg aaatcaacac ttgatctttt ttgaaaatat acgttgggac
     3061 ttcttgtcga agattatctt cccgtatacg agttcctgct gctgtagaat caacaaagga
     3121 ttttacgaaa agtgaaatgt ttttattttg aagtggtttg attgttttag ggtgaatcac
     3181 agtagcacca tagtatgcta attcgatcgc atcatagtat gataattcag gtaacaattg
     3241 tgcttgagga aattctcgtg gatcagcatt taaaacaccg ggtacatctt tccaaatggt
     3301 aacactttct gcatctaaac aataggctaa aattgcagca gaataatccg aaccttctcg
     3361 tccgagtgtt gtggtaaaat tctcagaagt accgccaata aaaccttgtg ttaagtatag
     3421 gagagaattt tttgttggtt gaataatttg agaaacagca tgcttcgttt tatcccaatc
     3481 aaccattcct tctcgatagg tattatcggt atgaattaaa tcacgtgcat cgagccattg
     3541 cgttttgatg ttttgatcat ttaaaaaagc tgcaacaatt cgggtagata aaaattcacc
     3601 ttgtgaaaca atttgatcgt attcgtaatc gtatgatttt atttgggttt cctcgattgc
     3661 ccaatataat tcagcaataa tagagtcgaa ttcctgttga agattagcgt tcttgttcgg
     3721 gaataaatca tctatgattc gctgatgcgt cgcttttact tcttctaaca atgcaggagc
     3781 ttctcctgtt tgatggaaaa atgcttgaca aactttttcc aatgcattgg tcgttttccc
     3841 cattgcagac acaacgataa ctaattgctc atccgtgtat ttgctaataa tagaagctac
     3901 gtttctaact ccatttgcat ctttaacgga tgcacctcca aacttaaaaa ccttcataaa
     3961 tcttctgcgg ctgtttgctg taattgttcg tagttgaatt tgcagttgat ccattccgca
     4021 tcaaactgtg cgttaaaacg cttataaaag ccaattgctg gctcattcca atcgagtacc
     4081 tgccattcca ttcttcctgc tttcgaattt ctggcaattt ggtaaacggc acgaaatagt
     4141 tcttttccga ttccgtaacc acgaaatggt tcttcaacta taatatcttc cagaaaaata
     4201 catgagcctt tccaagttga gtatttgtta taatacaagg caattccacg aatggattta
     4261 tcctccatac gagcaacaaa acagctatag attttattgg gaccaaatcc atcagctaat
     4321 aaaatagatt cggtgacggt tacttcattg gatgctcttt catattcggc taattgttta
     4381 attaaagcga gaatatgcgg cacatcatca ggagttgctt cttctataat ataattcatg
     4441 caacgaaatt aggctccaat aagatggagt gcaagaagct ttcaattaaa tcatgaacaa
     4501 ataaggtgaa taaaaaaagc ggctgcctct ttaaaagaaa cagccgcttt ataaaaaaga
     4561 ataaaattat gcttgagtag taggatttga agttggagtt gctgcaggag cagatgcttc
     4621 aggagctttt ttcacttcta cctttttagg ctcaaccttt tttgcaggtt ttacttttac
     4681 cgcaactggc tttttagctg gagctggttt tttcgcaatt ttaactgctt ttggtgctac
     4741 cggagttgat ggtttatctg attttacatc agctttaaca acaggttttg gagcatcaac
     4801 agttttaact gcttttacct ttggtgcctt aacagctttt actttaggag ctttaactgc
     4861 ttttactttt ggagcttttt cagctttcac tttaggagct tttgcagctt tcactttagg
     4921 agcttttgca gcttttacct taggagcttt tgcagctttt actttaggtg ctttaggtgc
     4981 ttttactgct gcttcttttt taactactgg tttagcagct tttttagcaa ctggtttaac
     5041 agctttctta gctgcttttg gagcactaac ttttgcagct tttaccactt tcgctttagc
     5101 aactttagtt gaagcttttt tagctactgc tggtttcgca cttgcttttg aacctggctt
     5161 gcgcccacgt tttttaggct cagttgcaac tacaggtgct gttgaactag caactactgg
     5221 acttgcagta acttttgccg gacgaccacg acgtttaggt gccgcttttg gtgcatcagt
     5281 tttcggttta gcaggacgtc cacgtttacg accagcaact ttcggagctg ctgcttttaa
     5341 gtctgcagaa atagacttag taagttgttt ttctgctgaa ggtttacggc cccttttttt
     5401 aggtgctgtt ccgattttag caataatgga cttaattgct tttaatctct cctccgcttt
     5461 ttccatttct tggatgtaaa atgcgtgaag ttcagacatt tcctcagcgg agaattcgat
     5521 gttttttgcc atataaattc atttattttg tggctaatat attttaaatc attaataaag
     5581 gttacattct tgatacacta atttcattaa ttaataataa taaattgcta actaactgat
     5641 tttgtgtgag attaatttta attaaaatga attatttttt tgcaagattt attgaattcc
     5701 tgttttttat ctaaatatca atcttcatat ccggtaattt attcctttta aaaaacattc
     5761 attctcaata agtaagacaa caaataaaat atcagataat agtccgaact tttctttcca
     5821 ataatatttt gattttaatc acttatcatg ttttcaaatc atgtttcatc agctatttct
     5881 tacatcgaat ttcaatttgt caaaatttag tttgctctat atcttccggt tttaaaggtg
     5941 ttcctgttat taatatacct gtaaaataat gtcgaagaat tgcttcagga ccaaagccta
     6001 attcggacat tcgtattgca ccttcttgac agagtcctac accatgccca aatcctttac
     6061 cataaaaata aacgctatcg tttctacact cccaatcaaa ccacgcagat tttaaatcaa
     6121 atcgagttcg taaatcaatc attcgaaatg ttctatttgc taattgaatt tgatgtggac
     6181 ggttttcttc aaaactgcaa atagaagtgc ataattcaag ttttgagatt gaatcggttg
     6241 gataagctat cgctaatttc tcacagaatt tttccagtgt tattcgtgta ttccaaaatg
     6301 catttttacc aactaaacaa aatgtatctt ctgtacttcg acaataggaa agtgtcgatt
     6361 tccaaacatc ttctgatgca tttgtaattc ctccacaatt agaatggaat aacaattcag
     6421 caagatttcc agtcaaacta tctttaacta caatattcct tgttgttaaa acagcaagtc
     6481 gatacggatc ataatcaaaa tagcctttgt aaacctgaca gtgggtttga tcacataatt
     6541 caaatccttc tgatttatgt cgccattgat aataacgtgc atacgttcgt gataaaatag
     6601 catgcacttg ataaagcgat actggtttat tgtatcctac ttcacctctt aaaacaccaa
     6661 ctaaataatc ggtttcattg acttgattaa taaatttaag ttttccagaa gaaacagaaa
     6721 ctacaatatc tccagcatag cgattccctt tataatccgg ttttagtggt ttaagaaaaa
     6781 gagcattccc tgaattagaa tgaaaacgca ctttgtttgc agttcctaaa accaatgaac
     6841 ttgttcggat ttcaagcgaa tttcctttta cttgaatctg aattttagct cccgattctt
     6901 ctccccaaat tcgaacacct ggatctattt ctagccatgt gttttctccc atttcaaact
     6961 ggacgaaagt aatttctgat gcgcgaaata atccaattcg aacttgttcc tcttgagcaa
     7021 tcaatgctct cgtcagaaaa cacaaaaaaa taatctggcg gtatacagac ataccacaaa
     7081 attaaaccag ccagaagcta ataaaacaag taattcagtg gtttataaac ataagatatg
     7141 tgtattcacg atttcattaa tcgcatcacc atatcttgag cagcacgttt agatgcacct
     7201 ggaccaccta gttttatttt caattcgctg aaatcctgta attgagcagt tctttcttta
     7261 ccttcttgaa tcaatgtact taaggtttgc tcaatttttc gtgcattcat atcatcttga
     7321 attaattctg gaactgccat tcgatccaat attaaattca ctagcgaaat gtattttacc
     7381 tttattaatc gacgcgctaa ccaatacgat agttgtccgc ctttataaca aacaacttct
     7441 ggcaccgaaa acaaggcagt ttcaagtgtt gcggtccccg aagtaaccaa tgcagcttgt
     7501 gaatgcgata atagctgata cgtttcacca gaaataactt ttacctgagt tcccccactc
     7561 cactcttggt attgagcagg atcgatagat ggagctcctg caataatcgg aatacaagta
     7621 tctaaacttt tgatgctttg taacatttcg ggaagcattt tggagatttc ctgcttgcgg
     7681 cttccgggta ataaagcaat tattggttga ttagtaatac cgtgtcgttg acgaaattca
     7741 gaatcgggca caaacgtttg aattgcgtct aataatggat gtccaacaaa tgtcacatct
     7801 acattaaatc gtgcatagaa atccttctca aagggtagaa taacatacat cctttctact
     7861 acttttcgaa tcttaaatac acgattttgc ttccaagccc atatttgagg agaaatataa
     7921 tagaatactc gaatggagtt ttcgtgtgca aactcggcga tgcgtaagtt aaaacctgga
     7981 taatcaatta agattaaaac atcggggttg aatgctagaa tatcggcttt acaaaaccga
     8041 atatttttaa cgattgtacc aatatttaat agtacttcag taaaacccat gaaagctaaa
     8101 tcgcggtaat gcttgacgat tgttgctcct tgtgattgca ttaaatctcc gccccagcat
     8161 ctgaattcgg cttgctcatc ttcttcttta atggctttga gcaaattcga tccatgcaaa
     8221 tcgcctgaag cttcacccgc aattacatag tacttcatat ctgacaaaga aaatgattca
     8281 ttggtagaat accactaaaa aatgggaatt ttcatgtagg tttgaaacat gaaagttaca
     8341 tttctaggga ctggtacttc tcaaggagtt cctgtgatag catgtacttg tgaagtgtgt
     8401 gcatcgactg atttacgcga caatcgttta cgctgttcaa ttttagttga aaccgaagca
     8461 ggatcattta ttattgatac tggtcctgat tttaggcagc aaatgttaag acaaaacgta
     8521 aaacgcttag atgcggttct atacacgcat gaacataagg atcatattgc aggtttagac
     8581 gatataagag cttttaattt cgtgctgcaa aaacacatgg atgtatatct aagtaattat
     8641 gtagaagatg cgctacgaag agaatttgca tacatattcg cggaagataa atatccagga
     8701 atacctttaa ttaaccttca tcggattgat gaaaacccat ttgtattatt gggaaaaacg
     8761 atcattccaa tcatggttag ccattacaaa atgcccgttt ttggttatcg tataggtgat
     8821 ttttgttatg tgaccgatgc caaaacaatt tcagcttctg aattggataa aatgaaagga
     8881 tgcaaagtaa tcgtgttaaa tgccttaagg aaagagacac atgtatcgca ccttacatta
     8941 gatgaagcaa tcgaaattct agaatatctg aagcctgaac aagcctatct gactcacatc
     9001 agtcaccaat taggcaaaca tcaagatatt gagcccgaat taccgagttt cattcgttta
     9061 gcttatgatg gcttgacaat cgaatgtagt tagttgggga tttatccaat caacaatact
     9121 tttccgtaaa caacgtgctt tttaccagat tggtcgatga agctgctgcg gaacatataa
     9181 caacctgaag catacatctt tcctgaactt gagtctttac ccgaccaagc ttgcttctta
     9241 tcatttgtta catatatttc tttgccccag cgatcgaaga taaccatgag gtaagttgaa
     9301 tccataattc cagtgacgac gacattaaaa gaatcgttta cgagatcgtc atttggagta
     9361 aaggcattag gagcaaaaaa ggtatataga aagtcaatgg taacttgttt cgatatagaa
     9421 tccttacaac caaaggaatt catcgctatg agctcaacat cgtaggttcc agatcctggg
     9481 aaaacataat cgggttcata ctcggttgaa atttcaccac ttccaaaatt ccacaagaaa
     9541 ttcgttgcat taatggattg attaacgaaa gaaaccgttg gaaaaatacc cggaattgga
     9601 gttgtatctg gagaaaaaac gaaatcaggt ataaggttaa atcggtaatc gggtatatta
     9661 actatagtag agtactgaca tccaaaactg tcggtaaccg ataattcata ggaaccagtt
     9721 aataaactat ctacaaaagt ggtatcgttt tgctgatcag taacattcca gaaataatta
     9781 tactcttgct gaattcctgg acgatcttca aatggggttc ctcctgttgc taatacaaat
     9841 gctgatccca atccaaagga gcaagtatct ggtagaataa caacttgaac ttgaaagtta
     9901 ttgatgtctt ctataaaaat agaatcccga tctatacatc ctaaagcatc aataacagaa
     9961 acatattgcc aaccgaatcc aagtccttga gctgtttggc ctgtttgtcc tgaaggactc
    10021 caagcataag caattggacc agtaccaaat tgagttgtca catcagcaat accattatta
    10081 gctcggcaat caaccaattg agtttgattt actgtttgaa tttcaacagc attaaaagtg
    10141 aagaaaatac tctgtgtatc gggatcaaaa tttccacaat tgtcctctac tcggccaaaa
    10201 ctttgaatct cataagatcc accttgcacc aattgttgtc ctaatagaag tgtaaattct
    10261 ttatcatata aacctccatt taagcaagca atacttgtaa tacctgtaag aggaatatca
    10321 actccgagtg gatcggtcaa gctaaaccca gtaacattaa cggtgttaca cttaacaaat
    10381 tcactaaaac taaatgtaat tgcggttgat ccacaactaa cttgagaagt aacagcttga
    10441 agtgttggag gagttagatc gataattcct gcagaagaaa tcgacatatc taattgatat
    10501 ccacattggt tttgt
//
LOCUS       DAOOQW010000064        10459 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_7942_length_10459_cov_4.639946, whole genome shotgun sequence.
ACCESSION   DAOOQW010000064 DAOOQW010000000
VERSION     DAOOQW010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 10459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10459
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7942_length_10459_cov_4.639946"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(53..937)
                     /locus_tag="PK281_05480"
     CDS             complement(53..937)
                     /locus_tag="PK281_05480"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 domain-containing protein"
                     /protein_id="HOY48833.1"
                     /translation="MKSMYRFLVAVLLVSFFTSCEREIEIELPNAENLLVVEGSIETG
                     QAPVVLVTRNRGFFKDFPTDLESFINEFVIQDALVVVSDGAVNDTLIFTVNILAYPYV
                     YYTSTNMVGEVGKTYSLTIVADGKTIRSHTTIPPAVSIDSTWFGLNPFDSSEDSLGVA
                     YLQMQDPDSMGNAYRLFAKKYSEFSFNQVAGSEFNDDYVNGQKVIFFAGQGEKPFVSS
                     DTFIPKEYFYNLGDTVILKFCSIGAKELKFFYTMDAALNSNGNPFAAPTLVKSNIEGG
                     LGVWCGYSPAFDTLIAIK"
     gene            complement(937..3306)
                     /locus_tag="PK281_05485"
     CDS             complement(937..3306)
                     /locus_tag="PK281_05485"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOY48834.1"
                     /translation="MISVLRNIIVLLLLLVFPNLIEAQTKHTLSGYVKDAATGEFLPG
                     ANVYLKEIMQGTSTNSYGFYSLTVPSGNYTLGVSFMGYTEYSAPVKLTSDLRFNVTLQ
                     PKVIETKVVEITEKRIENNIKSTDMGRVELSMDKMKKLPAFFGEVDLVKIVQLLPGVK
                     NAGEGNTGLYVRGGGPDQNLVLLDEAVVYNAAHLLGFFSVFNSDAVKTVDLHKGGMPA
                     QYGGRLASVLDISMKEGNSQKYKVDGGIGVIASRLTVQGPIKKDTASFIISARRTYID
                     AVTKPFIKSTSPTKGSGYYFYDLNTKINYRISDKDRLFLSGYFGRDVFEFKSTKSNFN
                     IRIPWGNAMISSRWNHLFSNKLFCNTSLIFSDYHFESNISQTAFNIKFASGVRDYNAK
                     LDFTWYPTIRHTVKFGANYIFHRFSPNNTSGDIAGEELNFGPKVYVFAHEAALYAGDD
                     FDITDKLRVHGGLRASWFAQVGPYTQYIKNEKDQKVDSVKYAAGDLIKQYPNVEPRLM
                     MRYTVNKNASIKASFTQNYQYIHITSLANQSLPTDLWFPSTARVKPQFGTQYSLGYYQ
                     NFKENTWETSIETYYKTMKNQIEFKDGTLPSSGVGDNLDNYLTFGSGEAYGTEFFVKK
                     ALGKTSGWVGYTLSWTKRTFPELNDGKTYFARYDRRHDLSVVISHELTSKWIFSAVFV
                     YASGNLLWLPTSVYFFEGKPVVNYGERNNYRMPAYHRLDLSATYTPVTTKKVKSSWNF
                     SIYNVYSRLNPYFIYVETTGSPLTGDLKNSAKKVSLFPIIPTVTYNFSF"
     gene            complement(3335..3709)
                     /locus_tag="PK281_05490"
     CDS             complement(3335..3709)
                     /locus_tag="PK281_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006257165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOY48835.1"
                     /translation="MQTESSLVTLVVGASTNPERYAYRAIEKLRAYNHPVLAFGLKEG
                     QVGDVTISKQLPSQTVIDTITLYVGPDRQADLIQPLLDLKPRRIIFNPGTENPSFIKQ
                     AEQAGVIAEVACTLVLLSLNEY"
     gene            complement(3855..5048)
                     /locus_tag="PK281_05495"
     CDS             complement(3855..5048)
                     /locus_tag="PK281_05495"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HOY48836.1"
                     /translation="MKLHAQNQSPVSKSPTFSFNNGLGFVSPDSSFGLSIRLRIQNRI
                     GATLTEMSDGTWQVTQNEFRVRRARVRFDGFVFNPKWTYAIQLSFSRADMDWDGSSVP
                     NVLRDAMIFYRPSKNWIIGFGQGKLPGNRQRVISSGEQQFVDRSIVNATFTIDRDFGI
                     NTSYTIPIGKPQIIVRAAITSGEGRNEGVVAYNKVNAGLCYTGRFEFLPFGAFDKRGD
                     YSEADLARETKPKLSLAGGWSYNEQTVRTGGQLGRSLLSPLNMNTLITDVLFKYRGLS
                     IYGEYVNRMSTTSPLTYSYDGNGKIIDTRWIYQGEGILGQAGYLFKNNYEIAARFAQI
                     TPQKEINNLEEIRKNYAICFSKYLKGHRLKLQSDFTLEELYDGQSKARNGRNIAWRFQ
                     IEMGL"
     gene            5339..5959
                     /locus_tag="PK281_05500"
     CDS             5339..5959
                     /locus_tag="PK281_05500"
                     /inference="COORDINATES: protein motif:HMM:NF013982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HOY48837.1"
                     /translation="MVPKNNLFFDLFAEDTANLVVQAKRFHELFSAKSPEEIQAIIED
                     VRILENKGDEITHKIFLELSANFITPFDREDIHFLASSIDDIADYINGSASRINLYKV
                     TEFTEPMRKLSEVIIKQAEDIHLAVSNLKGTKGTAKIKEAIVRINFLENQADDIFDNA
                     IAHLFAEEKDAIKLIKEKEVLSALETATDKCEDVGHALESILVKTT"
     gene            5973..7559
                     /locus_tag="PK281_05505"
     CDS             5973..7559
                     /locus_tag="PK281_05505"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HOY48838.1"
                     /translation="MDYPVFLVVIIALALIFDYINGFHDAANSIATVVSTKVLTPFQA
                     VVWAAFFNFVAFFIFKDHSVANTISKVVHEQFTSPELHPLPMIFAGLLSAIAWNLLTW
                     WLGIPSSSSHTLIGGFAGAFMAAFGIDAVRSGVVWATVIFIFIAPIAGMLMAYLISIW
                     FLNSFRKSIWMKLVSLSIIAGVVYLVWGALEFQKDFNGGSAYVLQFDAPIKKDELKKT
                     FTFKDDAGKEHTPVLKAGKDSLGQVDASGQVWVLRTSYLSKKGENEVNEKLSNSILAA
                     ASKSEHPVTITETHRITASTQPEERFDDSNLKSDFKPYWLKVVFHGSNFKWILLGFIV
                     VVMAVFTLFLSSFNNTKSNKWFRKLQLFSSAAFSIGHGGNDAQKVMGIISVALIANGN
                     IESLKDMPNWVPLSCYAAISLGTMSGGWKIVKTMGNKITKVTPFEGVAAETAGAITLF
                     FTEGIKFPWKIGDEVIKGIPVSTTHTITGSIIGVGITKRISAVKWGVTGKLLVAWIIT
                     IPISAAMGAIFYYICRLFGL"
     gene            7658..8773
                     /locus_tag="PK281_05510"
     CDS             7658..8773
                     /locus_tag="PK281_05510"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019669745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HOY48839.1"
                     /translation="MILAVPKEKKARENRVALTPEVVKELVGKGFSVLVERGAGERSY
                     LADSLYEAAGARMGDSQAVFSEADVVLAVNAPTSDDIARMKKGAVLISFLFAATAPET
                     VSALESAGISAFSMDAVPRISRAQKMDALSSQSNLAGYKTVLMAANALGKIFPMLMTA
                     AGTVKPAKVVIMGAGVAGLQAIATAKRLGAIVEVSDIRPETKEQVQSLGGYFIELKGD
                     DSMKTEGGYVKEVSEDFLRKQQELVGKHVAEADIVITTALIPGKKAPRLITEAMVKSM
                     KAGSVIVDMAVEQGGNCEISELNQTVVKHGVTLIGESNLPSLLPLNASQLYAKNISTF
                     LYHLADKDAFRWEMEEEITKGSLIVHQGKAVHPSVAK"
     gene            8806..9114
                     /locus_tag="PK281_05515"
     CDS             8806..9114
                     /locus_tag="PK281_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019669744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HOY48840.1"
                     /translation="MENILAFIQENIDMIYFIILSIFVGVEVIGHVPTVLHTPLMSGA
                     NAIHGVVIVGAIYVMLNLASDDYVSLALGFLAVLLGTLNVVGGFVVTDRMLEMFKKKK
                     "
     gene            9127..>10459
                     /locus_tag="PK281_05520"
     CDS             9127..>10459
                     /locus_tag="PK281_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019669743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HOY48841.1"
                     /translation="MNLSLLEICYLIASVTFVIGLKMMGNPKTARKGNLIGAAGMTTA
                     IFGTILLHEGEVPGIIYGLIALAILIGTVAGWVTARKVAMTKMPELVSLFNGMGGACA
                     ALISLIEYPHLSHDTAALIPVLAGLIIGSVSFSGSMIAFLKLNGTMNKAIRIPSYNII
                     NTILMIITLGLGAMMVINGDLNTTAFMYLLLALSLLYGVLFVVPIGGADMPVVISLLN
                     SFTGLAAAFGGFLYGNKAMLTGGILVGSAGTLLTVIMCKAMNRPLSNVIFGAFGGGSA
                     GVGASAEGGTVKDIQVADLAVLMNYSKKVIIVPGYGLAVAQAQHVIHEIELILESRGV
                     EVKYAIHPVAGRMPGHMNVLLAESSVDYGKLAEMEEVNPEFASTDVVLVVGANDVVNP
                     AAKTDPSSPIFGMPILEVENATHIIVNKRSMSAGYAGIDNQLFYNPKTSMLF"
BASE COUNT         3113 a         2013 c         2034 g         3299 t
ORIGIN      
        1 aaaaaaacgt ggaaattaaa aataggatcc tgttcatgct acaaatatac aattacttga
       61 tggcgattaa tgtgtcaaaa gcaggagaat atccacacca aacacctaat cctccttcaa
      121 tatttgattt tacaagagtt ggtgcagcaa aaggattccc attcgaattt aatgcggcat
      181 ccattgtgta aaagaatttg agctctttgg caccaatgga gcagaatttc aaaatcacgg
      241 tatctcccaa attgtagaaa tattccttag ggataaatgt atcagacgaa acaaaaggct
      301 tttctccttg tccggcaaag aagattactt tttgtccatt cacataatca tcgttaaact
      361 ccgaacccgc cacttgatta aaggagaatt cactgtattt ttttgcgaat aatcgatagg
      421 catttcccat cgaatcggga tcttgcattt gaagatatgc aactcccaaa ctatcttctg
      481 aactatcaaa tgggtttaat ccaaaccaag ttgaatcaat tgatactgct ggtggaattg
      541 tagtatggga acgaattgtt tttccgtctg ctacaatagt tagtgaatag gtttttccaa
      601 cttcacctac catgttagta cttgtgtaat acacataagg gtatgcgaga atattgacgg
      661 tgaaaattaa cgtatcgtta accgctccat cacttactac aacaagcgca tcttgaataa
      721 caaattcatt aataaaagat tccaaatcgg ttgggaaatc cttaaaaaat cctctattgc
      781 gagttaccaa aacaacagga gcttgacctg tttcaatact tccttctaca accaataaat
      841 tctcagcatt cggaagttca atctctattt cacgttcaca ggatgtgaaa aaactcacca
      901 acaaaacagc aactaaaaat ctatacatgg acttcatcag aaactgaaat tataggttac
      961 tgttggtatt attggaaaca aggaaacttt ctttgccgag ttctttaaat cgcctgttag
     1021 tggactaccg gttgtttcaa cataaataaa ataaggattc agtcggctgt aaacattgta
     1081 aatagagaag ttccagctcg attttacttt ttttgtagtg actggtgtgt aggtagctga
     1141 aagatccaat cggtgatagg caggcatacg gtaattgtta cgttcgccgt aattcacaac
     1201 tggttttcct tcaaagaaat aaacactggt tggcaaccat aataagttac ccgatgcata
     1261 aacaaaaaca gcactaaata tccatttgga tgttaattca tgacttatta caaccgataa
     1321 atcgtgtcga cgatcgtaac gtgcaaaata ggttttacca tcatttaatt cagggaaagt
     1381 ccgtttcgtc catgaaagtg tgtagccaac ccaaccactc gtttttccaa gtgctttttt
     1441 gacaaaaaat tcagttccat atgcttcacc agaaccgaaa gtcaggtaat tatcgagatt
     1501 gtcgcctaca ccactcgaag gtagagttcc atccttaaac tcgatttgat ttttcattgt
     1561 tttataatac gtctcgattg acgtttccca ggtattttct ttgaaatttt gataataccc
     1621 taaagaatat tgagttccga attgaggttt tacacgcgct gtactcggaa accataaatc
     1681 ggtaggaaga ctttgattgg ctaatgaagt aatatgaatg tattgataat tctgggtgaa
     1741 ggatgcttta atggatgcat ttttatttac cgtataacgc atcattaaac gaggctccac
     1801 attgggatac tgtttaatta aatcgccagc tgcatatttt acggaatcaa ctttttgatc
     1861 tttttcgttt ttgatgtatt gtgtgtatgg accaacctga gcaaaccaag aagctctcaa
     1921 tcctccgtga acacgaagct tatcggtaat atcaaaatca tcacctgcat ataatgctgc
     1981 ttcatgggca aacacataaa cctttgggcc aaaatttaat tcttctcctg caatatcacc
     2041 gctggtatta ttgggagaaa atcggtggaa aatataattc gctccaaatt taaccgtgtg
     2101 tcgaatggta gggtaccaag taaaatcaag ctttgcatta taatcacgaa cacctgaggc
     2161 aaacttaata ttgaaagccg tttgagaaat attggattcg aaatgataat ctgaaaatat
     2221 caaggaagta ttgcagaata acttattact aaaaaggtga ttccaacggg acgaaatcat
     2281 ggcatttccc catggaattc gaatgttgaa attgctcttc gtgcttttaa attcaaacac
     2341 atctcgtcca aaataacctg ataaaaacaa gcggtcttta tcactaattc gataattaat
     2401 tttggtattt aaatcataaa aataatatcc cgagcctttg gtaggagagg tgcttttaat
     2461 gaatggttta gtaactgcat caatgtatgt tctacgagct gaaataataa aagaagcagt
     2521 gtcttttttg atcggacctt gaacagttaa cctagatgca atcactccaa ttcctccatc
     2581 taccttatat ttttgactat ttccttcctt catactaata tccaacaccg atgctaaacg
     2641 acctccgtat tgagcaggca ttccgccttt atgaagatca accgttttta ctgcatccga
     2701 attaaatact gaaaaaaatc caagtaagtg agcagcatta tatacaaccg cttcatccag
     2761 taaaaccaag ttttgatcgg gaccaccacc acgcacatat aatcctgtat ttccttctcc
     2821 agcattttta acgcccggta gcaattgaac tatttttact aaatcgactt caccaaaaaa
     2881 cgcgggtagc tttttcattt tgtccatcga caattccacc cgtcccatat cggtactctt
     2941 gatgttattt tcgattcttt tttcggtgat ttcgaccact ttagtttcaa ttactttagg
     3001 ttgaagcgta acattgaaac gtaggtcact agtaagtttt acaggtgctg aatactccgt
     3061 ataacccata aaagaaaccc ccaaagtata attcccactt ggaacagtta gggagtaaaa
     3121 tccataacta ttggtggatg taccttgcat gatctctttc aagtaaacat tcgcaccggg
     3181 tagaaattca cctgtagctg catcttttac atatccactt aaggtgtgct tagtttgggc
     3241 ttcaattaaa tttggaaaaa caagaagaag caataagaca ataatgtttc gcaaaactga
     3301 tatcatataa ttatgggcaa cagatcaact ttatttagta ttcatttaat gatagcagca
     3361 ccaatgtgca agcaacttca gcaataacac cagcttgttc tgcttgttta ataaatgatg
     3421 gattttctgt ccccggattg aaaattattc tccttggctt taaatcgagt aggggttgta
     3481 taagatcagc ttgtcgatct ggtcctacat ataaagtaat ggtatcaatt acggtttgac
     3541 tgggaagctg tttgctaatt gtcacatctc caacttgtcc ttcttttaaa ccaaaagcta
     3601 aaacgggatg attataagca cgaagttttt ctatggcacg ataagcatat cgttcaggat
     3661 tagtgctagc gcctacaact aaagtaacaa gagatgattc tgtttgcata tcgagtgcaa
     3721 atatccactt atttcagttg tgaagtatca agttaattac atcaaaatta agcttttcat
     3781 aagaaattga ggatgcttgg aataatagga gtgtaggaac tagtatatca tcttaagcag
     3841 agaataatca gtacttataa acccatttca atttgaaaac gccaagcaat atttcgccca
     3901 tttcgagctt tagattgtcc atcgtaaagt tcttctaagg tgaaatcgga ttgtaatttc
     3961 aagcgatgtc cctttaaata tttcgaaaaa catatcgcat agttttttcg gatttcttct
     4021 aaattgttaa tctctttttg tggcgttatt tgagcaaatc gagcagctat ctcgtaatta
     4081 tttttaaaaa gatagccagc ctgtcccaaa ataccttctc cttgatatat ccatctggta
     4141 tctattattt ttccatttcc atcatacgaa taggttagtg gagaagtggt tgacatccta
     4201 ttcacatatt ctccataaat actcaagccg cgatatttga ataaaacatc cgtaattaag
     4261 gtgttcatat tcaagggtga taaaagagat cttcctagtt gtccgccagt tcttaccgtt
     4321 tgttcattat atgaccaacc tccagcaagc gacaatttag gtttcgtttc acgggctaaa
     4381 tcggcttcgg aataatcacc tcgtttatca aatgccccaa atggaagaaa ctcaaaccgt
     4441 cctgtataac ataaaccagc gttaacttta ttatatgcaa caacgccttc atttcgtcct
     4501 tcacctgatg taattgctgc tcttactatt atttgtggtt tgccaattgg aatcgtgtat
     4561 gaagtgttta taccaaaatc acgatcaatt gtgaaagttg catttactat tgatcgatcc
     4621 acaaattgct gttctccaga tgatataacc cgttgacgat ttccgggcaa tttaccttga
     4681 ccaaatccaa tgatccaatt tttacttggc cgataaaaaa tcattgcatc tcttaaaaca
     4741 tttggaaccg atgaaccatc ccaatccata tctgctctgc taaaacttaa ttgtattgca
     4801 taagtccatt taggattaaa tacaaaacca tcgaaacgaa cacgagcacg tctcacgcga
     4861 aactcgtttt gagttacttg ccaagttcca tccgacattt ctgttaaagt tgcacctatc
     4921 cgattttgta ttcttagtcg aatagaaagt ccgaaggagc tatctggact cacgaaaccc
     4981 aatccattat tgaagctgaa tgtaggagat ttgcttactg gggattggtt ttgtgcatga
     5041 agtttcacag tcaaaacgaa tagagttaaa aaaactgctc gaatgatgct tttggttgaa
     5101 aaaggtagat ttaacacagg attaacttat taatgactgg ctaacaaata gttaattttc
     5161 ctcgtctgga aattttgcgc aaatgtattg gattagattg gccgactcac ctcttgcaat
     5221 attaacttaa cattccctta attcagagaa cgcgtagaat taatatggta ggagttcttt
     5281 tgcggcaatt ctaaattaga ctctagaaat gatgcgcctg aacaaccttt tttcatttat
     5341 ggttccgaaa aacaatttgt tttttgacct gtttgccgag gatacggcta atttggttgt
     5401 acaagcaaaa cgcttccatg agctattcag tgctaaatcg cccgaagaaa ttcaagcaat
     5461 tattgaagat gttcgtatac ttgaaaacaa gggtgacgaa atcacacaca agattttttt
     5521 agagttatct gccaacttca tcactccatt tgatcgcgag gatattcact tcttagcctc
     5581 aagtattgat gatattgccg attacatcaa cggttcggct tctcgtatca acttatataa
     5641 agttaccgaa ttcaccgagc ccatgcgtaa actcagtgaa gtgattatta agcaggcaga
     5701 agatattcat ttggctgtat ccaatttgaa aggaacaaaa ggaacggcta aaatcaaaga
     5761 agccattgtt cgtatcaatt tcttagaaaa tcaagcggat gatattttcg acaatgcgat
     5821 agcgcatttg ttcgcagaag aaaaagatgc tattaagttg attaaggaga aagaagttct
     5881 tagtgcatta gagactgcaa ccgacaaatg cgaagatgtt ggacatgcgc ttgaatctat
     5941 tttggtgaaa accacttaat cgcttttgag ttatggatta tccggttttc ctagtcgtaa
     6001 ttattgcact agcgctaatt ttcgactata tcaatgggtt tcatgatgca gcaaattcaa
     6061 ttgctacggt tgtttctaca aaagttctaa caccgtttca agctgttgtt tgggccgcat
     6121 ttttcaattt cgtagccttc ttcatattta aagatcacag tgttgcaaat accatttcca
     6181 aagtggtaca tgagcaattc acttcgcctg agctacatcc tttacccatg atttttgccg
     6241 gattactctc ggctatcgct tggaacctac taacctggtg gctcggaatc ccgtcttctt
     6301 cttcacatac cttaataggt ggttttgcag gtgcttttat ggccgctttc ggtattgatg
     6361 ctgttcgttc gggtgttgtt tgggctactg taatattcat attcattgcc ccaattgccg
     6421 ggatgttaat ggcttacctg atttcaatct ggtttctcaa ttcattccga aaaagcattt
     6481 ggatgaaact agtctctctc tcaataattg ccggtgttgt ctatctggtt tggggagcac
     6541 tcgaattcca aaaagatttt aatggtggat cggcttatgt tctgcaattt gatgctccaa
     6601 ttaaaaaaga tgaactgaaa aagacattta cttttaaaga tgatgccggg aaagaacata
     6661 ctcctgtcct caaagctgga aaagatagtc ttggtcaagt agatgcttct ggacaagttt
     6721 gggttttacg tacttcttac ctttccaaaa aaggagaaaa tgaagtgaac gaaaaattaa
     6781 gtaattccat tctcgcagcc gcttctaaat cggaacaccc agttacaatt accgagactc
     6841 accgaattac cgcatcaact caacctgaag aacgtttcga cgattcaaat ctgaaatccg
     6901 atttcaaacc ttattggctg aaagtagttt ttcatggcag taatttcaaa tggattctat
     6961 taggatttat tgttgtggtg atggctgtct ttaccttgtt tcttagctca ttcaacaata
     7021 cgaaatccaa taagtggttc cgtaaacttc agcttttttc ctctgcagca ttcagtatcg
     7081 gtcacggtgg taacgatgca caaaaagtaa tgggtattat ttcagtagcg cttattgcta
     7141 atgggaatat tgaaagccta aaagacatgc ctaattgggt tcctttgtct tgctacgccg
     7201 ctatttctct tggaacgatg agtggtggtt ggaaaatcgt taagacaatg ggtaataaga
     7261 ttacgaaagt tactcctttc gaaggtgtag ctgctgaaac agcaggtgcc atcaccttat
     7321 tcttcaccga aggaataaaa tttccatgga aaataggaga tgaagtcatt aaaggtattc
     7381 cagtaagtac aactcatact attacaggtt cgattatcgg tgtcggaatc accaaacgta
     7441 tctcggctgt gaaatggggt gttaccggta aattactcgt ggcttggatc attactattc
     7501 cgatttctgc tgccatggga gccatctttt actacatctg ccgcttgttc ggactgtaaa
     7561 accctttatc cataatggtt ctaaactgtt aaaggataat ttcaaatttt gttgctgttg
     7621 ttgcatttct tctatttttg tcggaaatgc tacgcctgtg attcttgctg tacctaaaga
     7681 gaaaaaagcc cgtgaaaatc gggttgcatt aacacccgaa gtggtgaaag aacttgtcgg
     7741 aaaaggcttt tcagttcttg tggaacgagg agccggtgaa cggtcttact tagccgattc
     7801 attatatgaa gctgctggag cccgaatggg ggatagccaa gccgtttttt ccgaagccga
     7861 tgttgtactg gcagtaaacg ccccaacttc cgatgacatt gcccgcatga aaaagggtgc
     7921 tgtcttgatt agtttcctat ttgccgccac tgctcccgaa accgtttctg cactcgaaag
     7981 tgccggaatt agtgcattct ccatggatgc cgttccacgt atttctcgtg ctcagaaaat
     8041 ggatgctctc agctcgcaaa gtaacttagc tggttacaaa acggtgctaa tggctgctaa
     8101 cgccctaggg aaaatcttcc ctatgttaat gactgctgct ggaactgtta agccagcaaa
     8161 agtggttatc atgggtgctg gtgttgctgg cttgcaagca attgcgacag caaaacgttt
     8221 aggtgctatc gttgaagtaa gcgacattcg tcccgaaacc aaagaacaag tccaaagttt
     8281 gggtggatat ttcatcgaat tgaaaggcga tgattccatg aaaaccgaag gtggttacgt
     8341 gaaagaagtg tcggaagact ttttgcggaa acaacaagag ttggttggta aacacgttgc
     8401 cgaagccgat atcgttatta caactgcact tatcccaggg aaaaaagctc cacgcttaat
     8461 caccgaagct atggtgaaat ccatgaaagc cggatctgtc attgtggata tggcagttga
     8521 acaaggagga aactgcgaaa tctctgaact caatcaaaca gttgtaaaac acggtgttac
     8581 tcttattggt gaatcgaatc ttccatcttt attgcctttg aatgcaagtc agctgtatgc
     8641 taaaaacatc agtactttct tgtatcacct ggcggataaa gatgcattcc gttgggaaat
     8701 ggaggaggaa atcaccaaag gttcgttgat cgttcaccaa gggaaagctg ttcacccttc
     8761 ggttgctaaa taatcagcta ttcgcattct tttaaaattc attctatgga aaatattctt
     8821 gcctttattc aggaaaatat tgacatgatt tacttcatca tcctttcgat tttcgttggg
     8881 gtggaagtaa ttggtcatgt accaactgtt ttgcatactc cactcatgtc gggcgccaac
     8941 gcgattcacg gggtggtgat tgtgggtgcg atttacgtaa tgctcaattt ggcttccgat
     9001 gattatgtat cactcgcatt aggatttcta gccgttttgc taggaacatt aaatgtggtt
     9061 ggtggatttg tggtgaccga ccggatgttg gaaatgttta agaaaaagaa ataagagggc
     9121 cgaaccatga acttatcact actcgaaatc tgctatctga ttgcatccgt gacatttgtc
     9181 atcggattga agatgatggg aaatcccaaa actgcccgta aaggaaatct gatcggtgca
     9241 gctggtatga ccacagcaat ttttggaact atccttttac atgaaggaga agttcctgga
     9301 attatttatg gattaattgc cttggctatt ttaattggaa ctgttgctgg ttgggtaact
     9361 gcccgcaaag tagccatgac caaaatgccc gaattggtat ccttgtttaa tggaatgggt
     9421 ggtgcttgtg cagcattaat ctcactcatt gaatatccac atttatcgca cgatactgct
     9481 gctttaattc cggttttagc tggattaatc attggctctg tttcattttc aggaagtatg
     9541 attgctttct tgaaactgaa tggtaccatg aataaagcta ttcgaattcc ttcatataat
     9601 atcatcaaca caatcttgat gattatcaca ttaggtttag gtgcgatgat ggtgataaat
     9661 ggtgatttga atacaacagc atttatgtat ctgcttttgg cattgtcgct cctatacgga
     9721 gtacttttcg ttgtgcctat tggtggtgcc gatatgccag tggttatttc tttgttaaac
     9781 tcttttaccg gtttagctgc cgcctttggt ggattccttt atggaaataa agccatgctt
     9841 acaggtggta ttctcgttgg ttctgctgga acattgctta ccgttatcat gtgtaaagcc
     9901 atgaatcgtc ctttgagcaa cgttattttc ggagcttttg gcggaggttc agcaggagtt
     9961 ggggcttcgg ccgaaggtgg aacagtaaaa gatattcaag ttgcagattt agctgttttg
    10021 atgaattact cgaaaaaagt aatcatcgta cctggatatg gtttagctgt agctcaagct
    10081 caacatgtta ttcatgagat tgaactcatc ctagaatctc gtggtgttga agttaaatat
    10141 gctattcatc cagttgctgg tcgtatgcct ggtcacatga acgttcttct tgctgaatca
    10201 agtgttgatt atggtaagct tgccgaaatg gaagaagtaa atcccgaatt tgcttcaact
    10261 gatgttgttt tggtggttgg agcaaacgat gttgtaaacc cagcagctaa gaccgatcct
    10321 tcaagtccaa ttttcggtat gccaatccta gaagtagaaa atgctactca tattatagta
    10381 aataaacgtt ctatgagtgc tggttatgca ggtatcgaca atcaattgtt ttataatcct
    10441 aaaacaagta tgctctttg
//
LOCUS       DAOOQW010000065        10417 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_7972_length_10417_cov_5.709805, whole genome shotgun sequence.
ACCESSION   DAOOQW010000065 DAOOQW010000000
VERSION     DAOOQW010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 10417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10417
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7972_length_10417_cov_5.709805"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="PK281_05525"
     rRNA            <1..254
                     /locus_tag="PK281_05525"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            337..448
                     /gene="rrf"
                     /locus_tag="PK281_05530"
     rRNA            337..448
                     /gene="rrf"
                     /locus_tag="PK281_05530"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            520..592
                     /locus_tag="PK281_05535"
     tRNA            520..592
                     /locus_tag="PK281_05535"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:553..555,aa:Met,seq:cat)
     gene            812..1693
                     /gene="era"
                     /locus_tag="PK281_05540"
     CDS             812..1693
                     /gene="era"
                     /locus_tag="PK281_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013070280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HOY48842.1"
                     /translation="MPFQSGFVNIIGKPNAGKSTLMNALIGERLSIITPKAQTTRHRI
                     IGILNNPEYQIVFSDTPGIIRNPAYKLQESMNNFVEGSFQDADIFLYIAEVGENPTID
                     DIPEQLVNTTIPILFLLNKIDTGNQEQVEKKVQLWKGLLPTAEIIPVSALLNFNLDYI
                     LKRILHHLPEGPLYYEADALTDRSERFFITEIIREKILLNYQKEIPYSVEVAVEEFKD
                     EPEIARIQAVIYVSRESQKGIIIGHQGAMLKKVSTEARLAMESFLGKKVFLAVQVKVS
                     KDWRDSERQLKRFGYTS"
     gene            1696..3003
                     /gene="der"
                     /locus_tag="PK281_05545"
     CDS             1696..3003
                     /gene="der"
                     /locus_tag="PK281_05545"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002669503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HOY48843.1"
                     /translation="MGSIVAIVGRPNVGKSTLFNRITETRDAIVDEVAGVTRDRHYGK
                     ASWTGHDFTIIDTGGYVNGSDDVFEEEIRNQVKIAMEEADVLLFLVDITAGVTELDKE
                     VANIVRRSKTKTLLVANKVDNHERAALAGEFYSLGLGEVFCISSINGSGTGDLLDAIV
                     DGMEIVPEHEPEPEVPRIAVVGRPNVGKSSFVNALLGNDRNIVTPIAGTTRDTIHTRY
                     KAFGHDFYLVDTAGLRKKGKVHDDLEFYSVMRTVRAIESCDVCILMIDAQDGIEAQDV
                     NILWLALRNNRGVVILVNKWDLVEKDTNTTKKFEEMILDRIAPFKDVPIIFTSTTQKQ
                     RLLKAVESAVEVFENRKRHIQTRQLNDFLLPLIEDMAPPAVKGKYLKVKYVTQLKMHY
                     PAFVFFCNLPQYFKEPYKRFLENKLREKYNFKGVPITIFFRSK"
     gene            complement(3007..3714)
                     /locus_tag="PK281_05550"
     CDS             complement(3007..3714)
                     /locus_tag="PK281_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48844.1"
                     /translation="MSEHEVPENDAALTELFNEVQHVLSFSEKRDLETLQLFGLGSSV
                     SSGKNARFKEGSLHQYYREDQIRSICVRYRLRFLPVKLYSGEVPYEVVSLIKKHDHIY
                     TERDNQYYILASVSFFLAKDPYAAPLLFSVSEDGRYELICQWGKEMPWYIPIIKYPYR
                     DFRSMVFSSLIFGLTIVTLSSLLGHFDTSNIFKSILIQVPILVLFAGVFSTLALCWGL
                     VTHTDFSSDNWNSRFFR"
     gene            complement(3734..4282)
                     /locus_tag="PK281_05555"
     CDS             complement(3734..4282)
                     /locus_tag="PK281_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009779296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48845.1"
                     /translation="METTARAGMLVKHPIYGKGTIVKVDTTFYTIHFPNRGNMDISKR
                     TEDLELFEPVVETHSEEEEFSMSQMERTLRRLLNEYSDIQHTAPLADKWQGGTLIFQP
                     ADTNLKPKEVPIETFFHKIVMVRDRLRVMGQQINANSKLSDADKVDLQQYITRIYGSM
                     TTFNVLFKDSNHYFVGEKKNNE"
     gene            complement(4567..5817)
                     /gene="metK"
                     /locus_tag="PK281_05560"
                     /pseudo
     CDS             complement(4567..5817)
                     /gene="metK"
                     /locus_tag="PK281_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023567.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
     gene            complement(5912..6826)
                     /gene="gldA"
                     /locus_tag="PK281_05565"
     CDS             complement(5912..6826)
                     /gene="gldA"
                     /locus_tag="PK281_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     ATP-binding subunit GldA"
                     /protein_id="HOY48846.1"
                     /translation="MSIQVSSVTKLYGEQKALDTISFDVNPGEILGFLGPNGAGKSTM
                     MKIITTFIPPTLGEVKVCGFDIQSQSMEVRRNVGYLPEHNPLYLDMYVREYLGFIAGL
                     HQLSNKDKRITEMLGLTGLSNEQHKKIGALSKGYRQRVGLAQALIHDPKVLILDEPTS
                     GLDPNQLADIRELIRNIGKEKTLMFSTHIMQEVEAVCDRVIIINKGKLVADGKAGELG
                     NRSRGYSIHVEFDKEISRGALTGISGVKRVDKLTDQHWELFVEGSEDIRSEIFRFAVE
                     KQLTVLTINRHEQSLEEVFQELTRKNDN"
     gene            complement(7078..9549)
                     /locus_tag="PK281_05570"
     CDS             complement(7078..9549)
                     /locus_tag="PK281_05570"
                     /inference="COORDINATES: protein motif:HMM:NF008897.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     UDP-N-acetylmuramoyl-tripeptide:D-alanyl-D-alanine
                     ligase/alanine racemase"
                     /protein_id="HOY48847.1"
                     /translation="MNSYSIQTLAGWLGKEAMLAFPGTTTIHEILYDSRRINNPAASL
                     FVALVTSRNNGHRYIANLIRQGVCNFLIQEKPDEGLRTQANFILVSDTRIALQKIAAH
                     HRKQFEIPVLAITGSNGKTIVKEWLYESLKDDYQVVRSPKSFNSQIGVPLSVWQISTS
                     DQLAIIEAGISAPSEMVSLQEIILPTLGIFTNIGHAHAENFPSVQVHIQEKMKLFSQV
                     KTLVCCADHSLIIEEAKNQKINCLTWGKNPSADLRIVDEQIKGTGKQIQLVWQNSEFT
                     LSLPFVDAASVENALHTALMLLHVGLSIEKIKAKISQLHPVEMRLEFRKGIHQCTLIN
                     DTWSADLDSLRIALEAMEQQQQHQKRTLILSDILESGKSLEELYANVAELIKRKEVTR
                     FIGVGEQMLQFAPLFSGEKLFFPSTEALLAQLGSIGFQNECILLKGARIFGFERIASA
                     LQQKTHETVLEIKLSAMVNNLNFFRSKLSPGVKTMAMVKAFSYGSGSFEVANVLQFNG
                     VDYLAVAYADEGIELRNAGIALPILVLSPEVDSFISMIENRLEPEIYSFRMLELFSQA
                     VLARNGFDGQPIRIHIKFDTGMHRLGFEEAEIPELIRRLNEIPQLETVAAFTHLAASG
                     EAQHEAFTQEQISRFIRFTDALQQGIEKPFLRHVLNSSGISHYPQAQFDMVRLGIGLY
                     GIGDVEEQQHLEAVSKLSTSISQIREVKAGESVGYSRKGQVNQPSRIATLPIGYADGF
                     LRKLGNGRASVRVRGKQAFTIGNICMDMCMIDITHIPEAQEGDSVIIFEHAADIHQLA
                     NWLETIPYEVLTGVSERVKRVYYQD"
     gene            complement(9546..10157)
                     /locus_tag="PK281_05575"
     CDS             complement(9546..10157)
                     /locus_tag="PK281_05575"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019669272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HOY48848.1"
                     /translation="MFLENTLHSTKRRGSIEVICGSMFSGKTEELIRRLRRATFAKQR
                     VEIFKPAVDVRYHEVDVVSHDSNSIRSTPVESSQSILLLSGEVDVIGIDEAQFFDSEL
                     VNVCNELANMGIRVIVAGLDMDFRGFPFGPIPALLATAEYVTKVHAICMVCGNLANHS
                     HRFDNTNESLVLLGETDSYQPLCRDCFYERNPLPYTGSQARKS"
BASE COUNT         2925 a         2073 c         2235 g         3184 t
ORIGIN      
        1 aaagttgagt gaacctgact ctagtacgag aggaccgagt cggacgaacc tctagtgtgc
       61 ctgttgtcac gccagtggca ccgcagggta gctatgttcg gttgagataa gcgctgaaag
      121 catctaagtg cgaaactcat cacgagatta gctttctttt aagggtcgta ggagactact
      181 acgttgatag gctacaggtg taaaggcagt aatgtcatag ccgagtagta ctaattgccc
      241 gtaaactttc ttatctgaca gcgatttctt tgatttttac cttgtatttt ctttcttata
      301 tgtcactatc tgatcctttt tcaggtatta aggccttcag gtgactatag caatgaggtc
      361 cacctcttcc cattccgaac agagaagtta agctcattag cgcagatggt actgccataa
      421 ccggtgggag agtatgtcgt cgcccgattt ttttactaaa agccccttca ttggggcttt
      481 ttagttctaa gtattgagta atttactcaa atggtatatt gcggggtgga gcagttggta
      541 gctcgttggg ctcataaccc aaaggtcatc ggttcgagtc cggtccccgc tactaaaagg
      601 cctggttttt taaccaggct ttttttttaa ctcaaaattc agggcttgct tgaccattcc
      661 ctatatcata cctcgaaaga acgcctagat ttgatgttta acttggcctt tttgatcctt
      721 taaacacctt ttttctgttt tcatctaggg tattgttcac tttagttcga cctaagtagc
      781 tgctttggct atctttgcac aaataatcgt tatgcctttt caatcaggtt ttgtcaatat
      841 aataggtaaa cctaatgcgg gtaagtcaac tcttatgaac gcattgatag gagaacgatt
      901 atctattatt acccctaaag cacaaaccac tcgacatcgt attatcggaa ttttaaataa
      961 tccggaatac caaattgtgt tttcagatac acctggaatc attcggaatc ctgcttacaa
     1021 attgcaggaa tcaatgaata acttcgtgga aggaagtttt caagacgctg atatcttctt
     1081 gtatatcgct gaggtaggcg aaaatcctac aatcgatgat atccccgaac agcttgtaaa
     1141 tactactatt ccaatccttt ttcttcttaa taagattgat acaggtaatc aggaacaagt
     1201 agaaaaaaag gttcagctct ggaaaggcct tttgccaact gctgagatta tccccgtttc
     1261 ggctcttctg aattttaatt tggattatat ccttaaaaga attcttcatc atcttcctga
     1321 aggtccttta tattatgaag ccgatgcact tactgatcgt tctgaacgat tttttattac
     1381 tgaaataatt cgagagaaaa tcctcctaaa ttatcagaag gaaattcctt attcggttga
     1441 agttgctgtg gaagaattta aggatgagcc cgaaattgct cgtattcagg cagtaattta
     1501 tgtttcacga gaatctcaaa agggaataat aatcgggcat caaggtgcca tgcttaaaaa
     1561 agtttctacc gaagctcgct tagcaatgga atcatttctt gggaaaaaag tatttcttgc
     1621 tgttcaagta aaagtgagta aagattggcg cgatagcgaa cgccaactta aacgttttgg
     1681 ctataccagt taatcatggg aagtattgtt gcaattgttg gacgtccgaa tgtcggtaag
     1741 tctacactct ttaatcgtat tacagaaact cgagatgcca ttgtcgacga agttgctggt
     1801 gtaactcgtg atcgtcatta tggaaaagca tcatggaccg gtcatgactt tactattatt
     1861 gataccggtg gttatgtgaa cggttcggat gatgttttcg aggaagaaat tcgcaatcag
     1921 gttaaaattg ccatggaaga agctgacgta ttgctctttc tggttgatat tactgcaggt
     1981 gttaccgaat tagataaaga agttgccaat attgttcgtc gttctaaaac caaaacctta
     2041 ctcgttgcca ataaggttga taaccacgaa cgtgctgccc tagctgggga attttactct
     2101 ttaggccttg gagaagtttt ctgtatttca tcaataaatg gttctggtac aggtgattta
     2161 cttgacgcaa ttgtagatgg gatggaaatc gtgccagagc atgaaccaga accggaagtt
     2221 cctcgcattg cagtagtagg taggcctaat gttggaaaat catcttttgt caatgctctt
     2281 ttaggaaacg atcgaaacat cgtaacacca attgccggaa caacgcgaga tactattcac
     2341 acccgctaca aggcttttgg acacgatttc tacctggttg atacggctgg attacgtaaa
     2401 aaagggaaag tacacgatga ccttgaattt tattcagtca tgcgaacagt acgtgcaata
     2461 gaaagttgcg acgtatgtat actcatgatt gatgctcaag atggtatcga ggcccaagac
     2521 gtgaatattc tttggctagc acttagaaat aacagaggtg tcgtgatcct agttaataaa
     2581 tgggacttag tagaaaaaga cacaaataca accaaaaaat tcgaagagat gattttggat
     2641 cgtattgctc catttaagga tgttccaatt attttcactt cgaccaccca gaaacaacga
     2701 ttattaaagg cagtagaatc ggctgtagaa gtttttgaga atcgtaaacg ccacattcaa
     2761 acacgccagt tgaacgactt tcttctgcca ttgatagaag atatggcgcc tcctgctgtt
     2821 aaaggtaaat acttgaaagt gaagtatgtt actcaattga aaatgcatta ccctgcattt
     2881 gtatttttct gtaatctccc tcaatatttt aaagagcctt acaaacgttt cctcgaaaac
     2941 aaactccgag agaaatacaa ttttaaaggt gttccaatta ctatattctt ccgaagtaaa
     3001 taagttttaa cggaagaatc gactgttcca attatcggag ctaaagtcgg tatgcgtgac
     3061 taatccccag cataaggcta aggtagaaaa cacacccgca aacagtacca agattggtac
     3121 ttgtatgagt atgcttttga aaatattgga agtatcgaaa tgacctagaa gggaagaaag
     3181 ggttacgata gttagcccaa aaattaacga actaaacacc atacttctga aatctctata
     3241 ggggtattta ataatcggaa tataccaagg catttctttg ccccattggc atattaactc
     3301 gtatcggcca tcttcactta cggaaaaaag taaaggtgca gcataaggat ctttcgctaa
     3361 aaagaaagaa acggaagcca aaatgtaata ctggttgtct ctttcggtat agatatggtc
     3421 gtgctttttt atgagcgaaa ccacttcata tggtacctct cccgaataga gtttaacagg
     3481 aagaaagcgc aatcgatatc gaacacaaat agatcgaatc tgatcttcgc ggtaatattg
     3541 atgtaaagaa ccctctttaa atcgagcatt ttttccagaa gaaacactcg agccaagtcc
     3601 aaataattgt aaggtttcta aatcacgttt ttccgaaaac gacaatacat gttgaacttc
     3661 attaaagagt tcagttaatg cagcatcgtt ctctggaact tcgtgttcgg acatattaca
     3721 tcaacgctta gtcttactca ttgttcttct tttccccaac aaaatagtga ttactgtcct
     3781 taaacaatac attaaacgtt gtcatactac catatatacg tgtaatatat tgttgtaaat
     3841 caaccttgtc ggcatcactt agtttggagt ttgcgttgat ttgctgtccc attacacgta
     3901 atcgatcgcg caccatgaca atcttgtgaa agaacgtctc gattggaact tctttaggtt
     3961 ttaagtttgt atcggccggt tggaaaatca gcgttccacc ttgccactta tctgctaaag
     4021 gtgcggtgtg ttgaatatcg ctgtattcgt tcaataaacg tcttaacgta cgctccattt
     4081 gcgacatgga aaattcttcc tcctcagaat gagtttcgac aacaggttca aaaagttcta
     4141 agtcttcggt acgtttcgaa atatccatgt ttccacgatt gggaaaatga atggtataaa
     4201 aggttgtgtc gactttaacg attgttcctt ttccataaat gggatgtttg acaagcatgc
     4261 cggcgcgagc agtagtttcc atgaactgaa cgggttgatt tagggtgaaa aaaaagcccc
     4321 ggtggtagac cgaggctcga aaatacaaca gaaaaattat gctttagcag caactttttt
     4381 gctagcagct tttttagctg gagctttagc cgctactttt ttacttgcag gagcttttgt
     4441 agctgctttt tttgcatctg ctttaactgc aggtttttta gctgccgcct ttggggcttc
     4501 ttttttagca gctgctttag gagcttcttt ctttgctaca gccgctttag gagctgcttt
     4561 agaaccaatt ccaaaacctt ttttgaacgc ttcaacataa tcgagtttct cccatgggaa
     4621 caactcgact tttacttttt tctcgttctt tttgcctttg ttgaacactt tagtaaccgt
     4681 ttgtggttcg cggcccatgt gtccgtaagc agcagtttcg ctataaattg gagtacgtag
     4741 tttgaaacgt tgttcaatag cgtaaggacg catatctacc aatttagcaa cgatttgagc
     4801 gatttgtcca tcgttcattg ccactttagc agtaccatat gtgtttacat ataatcctac
     4861 tggttctgca acaccaattg cataagctac ttgaacaagc acttcatcag caacaccaga
     4921 agcaacaagg tttttagcaa tgtggcgagt agcgtaagct gccgaacggt ctactttaga
     4981 aggatctttt ccagagaatg caccaccacc gtgagctcct ttaccaccat atgtatctac
     5041 gatgatttta cgaccagtaa gtcctgtatc tccgtgtgga ccaccaatta caaacttacc
     5101 tgtagggttg atgtggtaaa caattttatt gttgaacaat gcctgaacac gtttaggcaa
     5161 tttcttaaga atgcgtggaa tcaagatatt gattacgtct ttcttaattt gctctaacat
     5221 tttcttttca gcatcgaaat catcatgctg agttgaaata acgattgcat cgatacgaac
     5281 tggctcattg ttatcattgt attcaatggt aacttgagat tttgcatctg gacgcaaata
     5341 tttcatttgt ttgttttcgc gacggatatc ggctaattca cgaagaagca aatgagctaa
     5401 ttccaatgga agcggcatat agttgtcggt ttcgcggctt gcataaccga acatcattcc
     5461 ttggtcacca gcaccttgtt tccaaggatc ttttttctca actccttggt tgatatcggc
     5521 tgattgttcg tggattgcag agaaaattcc gcatgaattt gcctcgaaca tgtattccga
     5581 ttttgtatac ccgattttac ggattacctc tcgggcaatt tcctgtacat caaggtatgc
     5641 ttcggattta acctctccag caagtacaac ctgacctgta gttacaagtg tttcgcaggc
     5701 tactttagaa gatggatcga atgctagaaa atgatcaatc agtgcgtcag aaatctgatc
     5761 ggcaactttg tccggatgtc cttcggacac tgattcggaa gtaaaaagat atggcatcta
     5821 tcgtgagttt taatggatga agcgggcgca aaaatagaga ttgctaggga cttaacaaat
     5881 cagaatgatg tgattgtgcg tgaattcgga attaattgtc gttttttcgt gttaattcct
     5941 gaaaaacttc ctctaaactt tgttcatgac gattaattgt gagaacggtg agttgttttt
     6001 cgactgcaaa tcgaaatatc tctgagcgaa tatcttcgct tccttcgaca aacaattccc
     6061 aatgttgatc ggtcaattta tctacgcgtt taactcctga gattccggtt aatgcaccgc
     6121 gtgaaatttc tttatcgaat tctacgtgaa tgctatatcc acggctccga ttgcccaatt
     6181 ctcctgcttt accatcggct actaatttgc ctttgttgat gataattacc cgatcgcaaa
     6241 cagcttctac ttcttgcatg atgtgcgtag aaaacatgag ggttttttct ttcccaatgt
     6301 tgcgaatgag ttcacggata tcggctaatt gattgggatc gaggccactt gttggttcat
     6361 cgagaatgag cactttagga tcatgaatga gtgcttgagc taaaccaaca cgttgacgat
     6421 atccttttga taatgcacca atttttttgt gttgttcatt gctcaatcca gtgagaccta
     6481 acatctcggt gatgcgtttg tctttgtttg ataattggtg caaaccagct ataaatccta
     6541 agtattctcg cacgtacata tctagataca aaggattatg ctcgggcaaa tatcctacgt
     6601 ttcgacgcac ctccatgctt tgcgattgga tgtcgaaacc acacactttt acttcgccca
     6661 atgtaggagg aataaaggtg gtgatgattt tcatcatagt tgattttccg gcgccgttag
     6721 gtccaagaaa gccaaggatt tcacccggat taacgtcgaa tgaaatagta tcgagtgctt
     6781 tttgttcgcc gtaaagcttg gtaactgaag aaacctgaat gctcacattt actgaattga
     6841 gttgcaaatt aagataaaag cacgggattg ctaaaaaaca gtttttatgc tttttcggaa
     6901 cagagtgctt tctgattaaa acgctacccc gcgcaaggga tagaaatgga aagcccgcat
     6961 cgtatgccaa agcttaagcg acggcataca gaggacttgg aatggatagc ccggttcatg
     7021 cgccacaaaa tacagaggac ttggagtgga tagcccggtt tatgcgccct aagaaactta
     7081 atcctgataa tagacacgtt ttactcgttc ggaaacgccc gtcaatactt cgtaaggaat
     7141 ggtttcgagc cagtttgcca attgatgtat atcggcagca tgttcgaaaa tgatgactga
     7201 atcgccttct tgagcttcgg gaatgtgcgt aatgtcgatc atgcacatgt ccatacagat
     7261 attgccaatg gtgaacgcct gcttaccacg aactcgcacc gaggccctgc cattaccgag
     7321 tttccggaga aaaccatcgg catatccaat gggtaatgta gcaatgcgtg atggttgatt
     7381 tacttgccct ttgcggctat aacctaccga ttctcccgct tttacttccc gaatttgaga
     7441 aatgcttgtg ctgagtttgc taactgcttc gaggtgttgc tgttcttcta catcgccaat
     7501 tccatatagt cctattccca agcgaaccat gtcaaattga gcttgtggat aatgtgaaat
     7561 gccggatgaa ttgagcacat gtcgaaggaa tggtttctct attccttgtt gaagggcatc
     7621 ggtaaatcga ataaatcgac tgatttgttc ttgtgtgaac gcttcgtgtt gtgcttctcc
     7681 acttgccgct agatgtgtaa atgctgctac tgtttcgagt tgtgggattt cattcaaacg
     7741 acgaattaat tctggtattt cggcttcctc gaatccgagt cggtgcatgc ctgtgtcgaa
     7801 tttgatgtgt atccgaatgg gttgaccgtc gaaaccgttt cgcgccagaa ccgcttgaga
     7861 aaatagttcg agcatacgaa aactgtaaat ctctggctca aggcgatttt caatcatcga
     7921 aataaagctg tcgacttcgg gactgagcac caaaattggg agcgcgatac cggcatttcg
     7981 gagctcgata ccttcgtctg cataagccac agcgaggtaa tctacaccat tgaattgcaa
     8041 aacgtttgct acttcgaagc taccactgcc gtatgaaaaa gcttttacca tcgccatggt
     8101 tttaacacct gggctcagtt tggagcggaa gaaatttaag ttgttgacca tcgcagaaag
     8161 tttgatttct aggacggttt catgtgtttt ttgttgaagt gctgaagcaa tgcgttcgaa
     8221 tccaaaaatg cgggctcctt tgagcaaaat gcattcgttt tgaaatccaa tagaaccaag
     8281 ctgagcaaga agtgcttcgg tgcttggaaa gaacaatttt tcgcccgaaa aaagtggggc
     8341 aaattggagc atttgttcgc caacaccaat gaatcgagtt acttcttttc gtttgattaa
     8401 ttcagccaca ttagcgtaca actcttcgag cgattttcca gattcgagaa tgtcggaaag
     8461 aatgagtgtt cgtttttgat gttgttgctg ttgttccata gcctcaagcg caatacgaag
     8521 cgagtcgaga tcggcactcc aggtgtcgtt gatgagtgta cattgatgaa ttccttttcg
     8581 aaactcgagt cgcatttcga ctggatgcaa ctggctgatt ttagctttta ttttttcgat
     8641 ggataagcca acatgtaaca acataagtgc tgtatgcaag gcattttcaa cagaagcagc
     8701 atcgacaaat ggaagtgaaa gtgtgaattc agaattctgc caaactagct gaatttgttt
     8761 gcccgtgcct ttgatttgtt cgtcgacaat tcttaggtcg gcagatggat ttttacccca
     8821 agtgaggcaa tttatttttt gatttttagc ttcttcaata atgagcgaat gatcggcaca
     8881 acataccaag gtttttactt ggctgaaaag cttcattttc tcttgaatat gaacctgaac
     8941 agaagggaag ttttcggcgt gtgcgtggcc gatattggta aaaatcccca gcgttggcag
     9001 aatgatttct tgaagtgaaa ccatttcgga aggagctgaa attccggctt cgataatggc
     9061 caattgatcg gaagttgaaa tctgccaaac cgataatgga acaccaattt gggagttgaa
     9121 acttttaggt gaacgaacta cttggtaatc atcttttagc gattcgtaaa gccattcttt
     9181 cacgatggtt ttaccattac ttcctgtgat agcgagaacc ggaatttcga attgtttgcg
     9241 gtgatgagcc gctatttttt ggagtgcaat tcgcgtatcg gaaactagaa tgaagttagc
     9301 ctgagtgcgt aatccttcat cgggtttttc ttgtatgaga aagttgcaaa cgccttgtcg
     9361 gatgaggttt gcaatgtatc gatgtccgtt atttcgggaa gtaacgagcg caacgaataa
     9421 gctagctgcc ggattattga tgcggcgact gtcgtaaaga atttcgtgaa tagtagttgt
     9481 tccgggaaaa gctagcattg cttctttccc cagccatcct gcgagtgttt ggatggaata
     9541 tgaattcatg atttacgcgc ctgtgaaccg gtgtaaggaa gaggattgcg ttcataaaag
     9601 caatcacgac aaagtggctg ataagaatcg gtttcgccca ataatactag gctttcgttg
     9661 gtattatcga aacgatgtga gtgattggca aggtttccac acaccatgca gattgcgtga
     9721 accttggtca catattcggc tgtggcgaga agtgctggaa ttggtccgaa aggaaatcct
     9781 cggaaatcca tatccaatcc tgcaacaatg acacgaatgc ccatattggc aagttcgtta
     9841 cacacattta cgagttcgga atcgaaaaat tgagcctcat caatgccaat tacatctact
     9901 tcgcccgaga gaagtaaaat actttgtgat gattctaccg gtgttgaacg gatcgaattg
     9961 gagtcgtgag agaccacatc aacctcgtgg taacgtacgt ctaccgcggg tttgaatatc
    10021 tcgactcgtt gtttagcaaa ggttgcacga cggagtcgac gaatgagttc ttcggttttt
    10081 cccgaaaaca tggaaccgca gattacttca atagaaccgc gacgtttggt ggagtgtaaa
    10141 gtgttttcga gaaacatgga agctaccgat atgtttttac gtatttttac ccggtggatt
    10201 gattccacaa aactaagtaa aagacattcg attcattact attgggataa acccttatgg
    10261 acgccaaccg catacacgac gatataaccc atctgcttta caaggtggaa gagcagtata
    10321 gccgtattct acaatacaac gattctattc cgatgatgga agtagatttg gctttgaaag
    10381 acgtaaggga tctttacgaa tcactgctcg acctacg
//
LOCUS       DAOOQW010000066        10407 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_7979_length_10407_cov_5.085684, whole genome shotgun sequence.
ACCESSION   DAOOQW010000066 DAOOQW010000000
VERSION     DAOOQW010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 10407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10407
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7979_length_10407_cov_5.085684"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /locus_tag="PK281_05580"
     CDS             complement(<1..551)
                     /locus_tag="PK281_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004570201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HOY48849.1"
                     /translation="MNEVVIIAAVRTPIGSFNGSLAGLTATQLGAAAIKGAVERAGIQ
                     PEQVQEVFMGNVISANLGQAPATQAATYAGLPDSIPSTTINKVCASAMKAVMLGAQSI
                     MAGTNDVVIAGGMESMSNIPYYLDKARNGYRLGHGQIIDGLVRDGLWDVYKNYHMGSA
                     AELCSREYSISREEQDAYAIESYT"
     gene            724..2766
                     /gene="ligA"
                     /locus_tag="PK281_05585"
     CDS             724..2766
                     /gene="ligA"
                     /locus_tag="PK281_05585"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014783914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOY48850.1"
                     /translation="MTQEEAKLRIAQLTEEINAHNYKYYVEAAPKISDREFDVLLEEL
                     QKLEKAFPELAALDSPTQRVGGSVTKNFDTVIHQVPFLSLSNSYSKEDLIDFDERIRK
                     SIDQEFTYVCELKFDGVAIGLQYSNGKLRQAITRGDGVQGDVVTANARTIGSIPLSLH
                     GSGFPEEFEIRGEVFMPLKSFEGLNKTRRAELEDLGYDEEQIADRLFKNPRNAASGSL
                     KMQESSEVAKRKLDCFMYFLIADGLDVQSHYDNLQLAKSWGFKISEHIRVCKSIDEIM
                     DFIDEWENKRDSLPFDIDGIVIKVNEYKVQRVLGNTAKSPRWAIAYKYKAQRVSTILE
                     KITYQVGRTGAITPVANLKPVFLAGTTVKRASLYNADYIAGMDIREGDRVFVEKGGEI
                     IPKVVEVDTSLRGMFSEPTQYITQCPECEATLIRKEGEAIHYCPNELGCPPQIKGKIE
                     HFIGRKAMDINSLGEKTIQALFDKGLIHTVADLYSLKFEDIFGQMEGFKETSTRNIIE
                     GIEASKKVPFERVLFAIGIRYVGETVAKKLALKFRNVDALMQATKEELLDTEEIGEII
                     ALSVLEYFSVAEHREQIEKLRQAGLQLELDELAHQPKSDRLQGKSFVVSGVFSVFSRD
                     ELKKTIEENGGKVLSGVSASTTYLIAGEKMGPEKRKKAEKLQVAIITEQEFIEMIS"
     gene            2763..3362
                     /locus_tag="PK281_05590"
     CDS             2763..3362
                     /locus_tag="PK281_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48851.1"
                     /translation="MSVIDKLKLSYAHMQLKREVANISRDRTDPDIHRAGCIGILYDA
                     TDRDTFEFVREFYKDLKDSHKSPVSLGYIDFKETLSFHPLARPEADYFFKSQVNWLQK
                     PNSAVVDNFINEPFDILINLTLQDNYSLDYIAAVSKAGLKIGRAQSAVAFCYDLTFQL
                     DESADLKTFAYTIVHYLSQINHEHTSNHKRKRSSHYYTV"
     gene            3307..4218
                     /gene="dapA"
                     /locus_tag="PK281_05595"
     CDS             3307..4218
                     /gene="dapA"
                     /locus_tag="PK281_05595"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006260226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HOY48852.1"
                     /translation="MNTHPTIKGSGVAIITPFDPKGNVDEHALRRLVNHLAEGGIDVL
                     VALGTTGESVTLSKDEKKRVQEIVLEENAGRCAVMLGVGGNNSHEVVETLQHTDTNGI
                     SAILSVAPYYNKPNQEGHFQHYKLVAEASAKPVMLYNVPGRTGVNMTAETTLRIAHEC
                     KNVCGVKEASGNMEQIMQIIRQRPDGFLVHSGDDALTLPLLACGADGVISVVANAYPL
                     LFSEMTRRAMDGDVVSARPIHYALLEFTRLMFADGSPAGIKSALKLLGICDEYVRLPL
                     VNVRSDIYGAIEREMQHIKHFHLQATR"
     gene            4253..5656
                     /gene="glmM"
                     /locus_tag="PK281_05600"
     CDS             4253..5656
                     /gene="glmM"
                     /locus_tag="PK281_05600"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013996759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOY48853.1"
                     /translation="MALIKSISGIRGTIGGAPGDGLSPVDIVKFTSAFATWVKQRQQR
                     DHYTIVIGRDARISGEMFRNLVVGTLQGMGIDVIDLGLSTTPTVELAVEMENAHAGII
                     LTASHNPKQWNALKLLNEKGEFISAEDGQNVLDIADREDFNYVDVNHLGSYHQVDGYM
                     RKHIDRVLALPLVDVEAIRNANFRVVIDAVNSTGGIAIPMLLKALGVEQVTELYCTPD
                     GKFPHNPEPLPEHLRDLSEEVVKKKAHLGICVDPDVDRLALVCEDGDMFGEEYTLVAV
                     ADYVLKNTPGSTVSNLSSTRALREVTEKYNNQYFASAVGEVNVVEMMKAQNAIIGGEG
                     NGGIIYPELHYGRDALVGIALFLTHLAKYGKSVSMLRSTYPNYHISKNKVELTPELNV
                     DEILVKVQEKYKKQPINTIDGVKIEFDKSWVHLRRSNTEPIIRIYSEAENMATADNLA
                     NKIIMDIKEFTAEMMLK"
     gene            5707..6849
                     /locus_tag="PK281_05605"
     CDS             5707..6849
                     /locus_tag="PK281_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013751616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HOY48854.1"
                     /translation="MAIYLDNAATTRLDDEVIDAMLPLMREQFGNPSSIHSLGRITRS
                     AIEKSRKTVASLLNVSPSEIFFTSGGTEANNMAIVRSIEDFGLKHAITSPTEHHAVLH
                     TLEHLAEEGKIELSILNVDEKGNVDLAQLEELLKTKPRSLVSLMHANNEIGTRLPLKE
                     VATLVKSYNGIMHCDTVQTMGHYKFDLQEIPVDFINCAAHKFHGPKGVGFIFIRSGSN
                     IHPFIYGGAQERNMRGGTENLYGIVGLAKAMEVAYRDLEAHQQHVQGLKNYMIEQLRE
                     SIPGVQFNGDISENSLYTVLNVSFPPNESGDMLLFNLDINGICASGGSACSSGSDIGS
                     HVLGALKVAPTRHNVRFSFSKYTTKEEIDTTVNKLKEILQPVSVSS"
     gene            6854..7873
                     /locus_tag="PK281_05610"
     CDS             6854..7873
                     /locus_tag="PK281_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HOY48855.1"
                     /translation="MSDQPSAHMPSVAVIGAGSWATAIVKILNERPIHVHWWMRSEEQ
                     VQHIAQFSHNPRYLSDIQLDMNKVHPSSDLSAVIAGSEMIILALPAAFVHQTLSGLSK
                     DAFHGKSVFSAIKGMIPERHENITDYLHTSIGIHPKQMGIIAGPCHSEEVAMERQSYL
                     TVSAVDEELALRMAHILNCRFIHTHTILNDVQGIEYATVMKNIISLAVGITRGMNYGD
                     NFQAVLVSNAMQEMKRFLDAAVPNANRDLNESAYLGDLLVTAYSQFSRNRIFGQMIGK
                     GYTVKSAQLEMNMIAEGFYAVHSLNEVNKSLGVDLPICDFVHRILYEGGSPSREIRVL
                     EAKLK"
     gene            complement(8042..>10407)
                     /locus_tag="PK281_05615"
     CDS             complement(8042..>10407)
                     /locus_tag="PK281_05615"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOY48856.1"
                     /translation="LFYYVLEVRHSPEGAFETATSVTMGQPGSTVITEPLGNYPLENY
                     QVRQYSIQAAGDLDDDGRNDVVELLAIPNQSPLNAAQSTLINDGLVAVDQFTKFKELS
                     ITRELVPWNEYLNGKVFVKYMIADFYTDHPKLYFINTNTHPLHENFAYAIGIDFVSSE
                     INTGHIIYHPSTISNNGTLGTFAFNFSGGNYEDFSQVQKVHEMLAANMPFIRNNLSYF
                     VIEQNESQFAADSLLFEASRVPVLLESDVYADVNYWGLNPAEGFGFFRQLATGEVPGA
                     KDIVLYESLPTTLPRVGGIITSVIQTPLSHVNLRAIQNHIPNAFIRDPLLIDSIAALM
                     NHYIYFKVEQERYFIREATLDEVNAWHENLRPLTEQIPPLNLTYTSVLPLSEITFNMF
                     DGFGAKCANVATMRTFGFPEGTIPDGFGVPFYFYQEFMSYNNLFEEIETIMANPAFIS
                     DRNVRDDLLDDFREQIEDAQMPPWMLTELSAMQTSFPVGTSIRCRSSTNNEDLPGFNG
                     AGLYDSKTQHPDEGHISKSIKQVYASLWNLRAFEEREFYRINHFKASMAVLCHPNHED
                     EKVNGVGVSTDPIYNSENTFYLNSQVGDDLITNPGANTIAEEILLDRVSMSENDFIII
                     QHSNLVPADSVIMTEEYLEQMRSYFSVIHDQFEVLYKAVGNSTFAMDIEYKITSDDKL
                     VIKQARPWVSYVYQEQIPESEGEHYKLEVFPSPASSYISIYCRDCEIGILKIYDVQGR
                     LVHEQSIANPGNVVQNIAVGHLNAGFYIVSGTSADEKNRYSRRFMKN"
BASE COUNT         3082 a         2079 c         2292 g         2954 t
ORIGIN      
        1 gtatacgatt caattgcgta agcatcttgt tcttcacggc taatgctgta ttcacgtgaa
       61 cacaattccg cagctgaacc catgtggtag tttttataca catcccacaa accatcacgt
      121 accaaaccat caatgatttg tccgtgaccc aaacgataac cattacgtgc tttatcgagg
      181 taatacggta tgttcgacat gctttccatt ccaccagcaa taaccacatc attggttccg
      241 gccatgatcg attgagctcc caacatgacc gctttcatag ccgaagcaca tactttatta
      301 atagtagtac taggaattga atcaggtaaa ccagcgtaag tagccgcttg agtagcaggt
      361 gcctgaccta aattggcaga aatcacatta cccataaata cttcttgtac ttgttccggc
      421 tggattccag cgcgttcaac agcacctttt atagcagcag cacctaactg tgttgcagta
      481 agtccggcca aagagccgtt aaaactacca atcggagtac gaacagcagc aatgattaca
      541 acttcattca tgatttggga aaacgagggt taaaaatcgc tgcaaactta tgaaaagttc
      601 attagtagtg tgctaatatg ctaatatgct aatgtgctga tgtgctgtta aattattccg
      661 actgtttata aaatgaatca ccatgctcta ttatttctca ctgcattctg tgagatttgc
      721 aacatgactc aggaagaagc aaaattgcgg atagcgcagt tgaccgaaga gataaacgca
      781 cacaattaca agtattatgt ggaggctgca ccgaaaattt cggatcggga atttgatgtc
      841 ttgttggagg aattgcaaaa gctagaaaaa gcatttcccg aattagcagc actcgattct
      901 cctacgcaac gtgttggtgg aagtgtgact aaaaatttcg acacggttat tcatcaagtt
      961 ccatttcttt ccttatcgaa ttcatactcg aaagaggatt tgattgattt cgacgaacgt
     1021 atccgcaaaa gcatcgacca agaatttacc tatgtatgtg agttgaagtt tgatggagtt
     1081 gctattggat tacaatattc aaatggaaaa cttcgtcagg ctattacgcg gggcgatggt
     1141 gttcagggtg atgttgttac tgcaaatgct agaacaattg gaagtattcc acttagtcta
     1201 catggatcgg gttttccgga ggaatttgaa attcggggcg aagtctttat gccactcaaa
     1261 agcttcgaag gattgaataa aacccgtcgt gccgaattag aagatttagg ttacgatgaa
     1321 gaacaaattg ccgatcgtct tttcaaaaat ccgcgtaacg cagcttctgg aagtctcaaa
     1381 atgcaagaga gttccgaagt agccaaacgg aagctcgatt gtttcatgta tttccttatt
     1441 gctgatggat tggatgtcca aagtcattat gacaaccttc agctagctaa gtcatgggga
     1501 tttaagattt cggagcacat tcgagtgtgt aaaagcatcg atgaaataat ggattttatt
     1561 gatgaatggg agaacaaacg cgattcttta ccattcgaca tcgatggaat tgtgatcaaa
     1621 gtgaatgaat ataaggtgca acgcgtttta ggtaacaccg caaaatctcc acgctgggcc
     1681 attgcataca aatacaaagc acaacgagtt agcaccattc tcgagaaaat cacttatcaa
     1741 gttggtcgta caggagcgat cactccagtt gctaatctaa aaccagtatt cctcgctgga
     1801 acaaccgtta aacgtgcatc cttatacaat gccgattaca tagccggaat ggatatccgc
     1861 gaaggcgatc gggtatttgt ggaaaaaggt ggagaaatca ttcctaaagt agtcgaggtc
     1921 gatacttcac ttcgtggaat gttcagcgaa cctacacagt acattaccca atgccctgaa
     1981 tgtgaagcaa cgctcattcg taaagaaggt gaggcaatac actattgtcc gaatgaattg
     2041 gggtgcccac ctcagataaa aggtaaaatt gagcatttca ttggtcgtaa ggcaatggat
     2101 atcaatagtt tgggcgagaa aaccattcaa gctttattcg acaaaggttt aatacatacc
     2161 gtggctgatt tatacagctt gaagtttgaa gatattttcg gacagatgga aggattcaaa
     2221 gaaacatcta cccgaaatat tattgaagga attgaagctt cgaaaaaagt tccttttgag
     2281 cgcgttttgt ttgcaatcgg aattcgttat gtaggcgaaa cggtagctaa aaaactagca
     2341 cttaaatttc gaaatgtgga tgctttgatg caggctacaa aagaagaatt acttgataca
     2401 gaggaaatag gggagattat agccttgagt gtgctcgaat attttagtgt tgccgaacac
     2461 cgtgagcaaa tcgagaaact tcgccaagct ggattacagc tcgaattgga tgaattggca
     2521 catcaaccta aaagtgaccg attgcaagga aaaagctttg tcgtatccgg tgtttttagc
     2581 gtattttcgc gcgacgaact caagaaaaca atcgaagaga acggcgggaa agttttaagc
     2641 ggagttagtg catccacaac ctacctcatt gcaggtgaga aaatgggacc agaaaagcgc
     2701 aagaaagctg agaaactcca agtcgcaatc atcaccgaac aagagttcat tgagatgata
     2761 tcatgagtgt tatcgataag ctgaaactct cgtatgccca catgcagctg aaacgcgaag
     2821 tagcgaacat cagccgcgat agaaccgatc cggatattca ccgggctggc tgtattggta
     2881 ttttatacga tgccaccgat cgtgatacat tcgaatttgt tcgtgagttt tataaggatt
     2941 tgaaagacag tcacaaaagt ccggtatcat taggatatat cgactttaaa gaaacgcttt
     3001 cctttcatcc gctagcgcga cctgaagctg attacttctt taaaagtcag gttaattggt
     3061 tgcaaaaacc aaatagtgcc gtagtcgata attttatcaa cgagccgttt gatatcttaa
     3121 tcaatctcac gttacaagac aattattcat tggattacat tgcagcagtt tccaaagccg
     3181 gactcaagat tggtcgggct caaagtgcag ttgcattttg ttacgattta acttttcagc
     3241 tcgacgaatc agccgatttg aagacctttg cttacacgat agttcattac ctaagtcaaa
     3301 tcaaccatga acacacatcc aaccataaaa ggaagcggag tagccattat tacaccgttt
     3361 gatcctaaag gaaatgttga tgaacacgct ctgcgtcgcc ttgtaaatca cttggctgaa
     3421 ggtggaattg atgtgcttgt tgctcttgga actacaggtg agtcagttac tttaagcaaa
     3481 gacgaaaaga aacgtgttca agaaattgta ttagaggaaa atgctgggcg ttgtgctgtc
     3541 atgttaggtg ttggaggaaa caattcgcat gaagttgtag aaacgcttca acataccgat
     3601 acgaatggca tttcagctat tttatcggtt gctccttatt acaataaacc aaatcaagaa
     3661 ggtcattttc agcattataa actagtagct gaagcgagtg ctaaaccagt gatgctttat
     3721 aatgttccag gtagaacagg cgtaaacatg actgccgaaa caaccttgag aatcgcacat
     3781 gaatgtaaaa acgtgtgtgg cgtgaaagaa gcttctggaa acatggagca aatcatgcaa
     3841 ataattcgtc aacgtcccga tggattcctg gttcacagtg gcgatgatgc tttaacgctg
     3901 cctttattgg cttgcggtgc cgatggtgtg atctcggttg tagctaatgc ttatccttta
     3961 cttttctctg aaatgactcg tcgcgcaatg gatggagatg ttgtttcagc tcgtccaatt
     4021 cattacgcat tactggagtt tactcgattg atgtttgcag atggaagtcc agcaggtatc
     4081 aaatcggcac tcaaattatt gggcatttgc gatgaatatg tgcgtcttcc tttagtcaat
     4141 gtacgctccg atatttatgg ggcaattgag cgggaaatgc agcatatcaa acactttcac
     4201 cttcaagcga ctcgataagt caactgaatt ttaaattatt attccaacag tcatggcatt
     4261 aattaaatca atttcgggaa ttcgtggaac cattggtggt gctccgggag acggactttc
     4321 acctgtagat attgtcaagt ttacttcagc ttttgctact tgggtaaaac agcgccaaca
     4381 acgcgatcat tacacaattg ttattggtcg tgatgctcgt atttcgggag aaatgttccg
     4441 caatctggtt gttggtacct tgcaaggaat gggcatcgac gtgattgatt tgggcttaag
     4501 taccactcca actgttgagt tagcagttga aatggaaaat gcgcatgcgg gaattatcct
     4561 tacagctagc cacaatccta aacaatggaa cgccttgaaa ttgctcaacg aaaagggcga
     4621 attcatctct gcggaagatg gtcaaaatgt actcgatatt gctgacagag aagattttaa
     4681 ctatgtggat gtaaatcatt taggtagcta tcatcaagtg gacggataca tgcgtaaaca
     4741 catcgaccgt gtattagcct tgccattggt ggatgtggaa gctattcgta atgccaattt
     4801 ccgagtggtg attgatgcag ttaattcaac tggaggaatc gctattccaa tgttattaaa
     4861 ggctttagga gttgaacagg tgacagaatt gtattgcaca cctgacggaa aattccctca
     4921 taatccagaa ccgctaccag agcatttgcg cgatttgagc gaagaagtgg tgaaaaagaa
     4981 agctcatttg ggtatttgtg tagatccaga tgtggatcgt ttagctcttg tatgtgaaga
     5041 tggagacatg ttcggtgagg agtacacctt ggttgctgtg gccgattatg ttttgaagaa
     5101 cacacctggt tctactgttt caaatttgtc ttcaaccaga gcattacgtg aggtaactga
     5161 aaagtataat aatcagtatt ttgcgtcagc agtaggggag gtgaatgtgg tagaaatgat
     5221 gaaagctcaa aacgccatta ttggtggaga aggaaatggt ggaattatct atccggaatt
     5281 acattacgga cgagatgctt tagttggaat cgctttgttc ctcacgcatt tagctaaata
     5341 tggtaagagt gtttcgatgc ttcgatcaac ttatcctaac tatcatattt ctaagaacaa
     5401 agttgagttg actcctgagt taaatgtgga tgagattcta gttaaagttc aggagaaata
     5461 taaaaaacag ccaatcaaca cgatagatgg ggtgaagatt gagtttgata aatcttgggt
     5521 gcatttacgt cgttcgaata cagagccgat tatccgcatt tattccgaag ccgaaaacat
     5581 ggcaactgcc gataacctag cgaataaaat catcatggat atcaaggagt tcacggctga
     5641 aatgatgctg aaataaacct attgttcaac caacaggtcg ttgcagcggc cttttcccaa
     5701 aatactatgg caatctatct cgacaatgca gcaactactc gcttagatga tgaagtcatt
     5761 gacgcaatgc ttcctttgat gcgcgagcaa tttggtaatc cttcctcgat acattcttta
     5821 gggcgtatta ctcgttcggc aattgagaaa tctcgtaaga cagtagcttc tttgctaaat
     5881 gtttcaccat cggagatttt ctttacatcc ggcggcaccg aagcgaataa catggcaatc
     5941 gttcgaagca ttgaagattt tgggttaaag cacgccatta cttctcccac cgaacatcat
     6001 gcagtattgc atactttgga gcatttagcg gaagaaggaa aaattgaatt gagcatctta
     6061 aacgtcgatg aaaaaggaaa tgtcgatcta gctcaattag aagagttatt gaaaaccaaa
     6121 ccgcgttcat tggtgtcgct tatgcacgcc aataacgaaa ttggaactcg tttgcctttg
     6181 aaagaagttg ccactctggt taaaagctat aatggcatca tgcattgtga taccgtgcaa
     6241 acaatgggtc attataaatt tgatttgcag gaaattcctg tagattttat caattgtgct
     6301 gcgcataaat ttcatggtcc caaaggagtt ggattcattt ttatccgttc gggatcgaat
     6361 attcatccgt tcatttatgg tggtgctcaa gaacgcaata tgcgtggtgg aaccgagaat
     6421 ctatacggaa ttgttggatt agctaaagcg atggaagtag cgtatcgcga cttagaagcc
     6481 caccaacaac atgtgcaagg tttgaagaac tatatgattg agcaattgcg cgaaagtatt
     6541 cctggtgtgc aattcaatgg tgatatttca gaaaactcgc tttacacggt gttgaatgtg
     6601 tcatttcctc cgaatgaatc gggtgatatg ttgctattca acctcgacat caatggtatt
     6661 tgtgcttcag gaggaagtgc ctgttcttct ggttctgaca ttggttcgca tgttttaggt
     6721 gcattaaaag ttgctccaac acgtcacaat gttcgtttct catttagtaa atacaccacc
     6781 aaagaagaaa tcgacacgac tgtaaataaa ctcaaggaaa tccttcagcc ggtttcggtt
     6841 tcatcttaag cctatgagcg atcaaccatc cgctcacatg ccttcggttg ccgtaattgg
     6901 cgcaggaagt tgggctacag ccattgttaa gatattaaac gaacgtccca ttcatgttca
     6961 ttggtggatg cgttccgaag agcaagttca gcacattgcg cagttttctc acaatccgcg
     7021 ctacctgagc gacattcagc tcgacatgaa caaagtgcat ccttcttcgg atttatcagc
     7081 tgtaatagct ggctcggaaa tgatcatttt ggctttgcca gctgcttttg ttcatcaaac
     7141 cttgtcggga ttatctaaag atgcttttca tgggaaatcg gtattctcag ccatcaaagg
     7201 catgattccc gaacggcacg aaaacattac tgactatctg catacaagca tcggcattca
     7261 tcccaaacaa atgggtatca tcgccggacc atgtcactcc gaagaagtag caatggaacg
     7321 tcaatcatat ttgactgttt cggctgtgga tgaggaattg gctttgcgca tggcgcatat
     7381 tctcaactgt cggtttattc atacgcacac gattttaaac gatgtgcaag gaattgaata
     7441 tgcgacggtt atgaagaata ttatctcttt agctgttggt attacccgtg ggatgaatta
     7501 tggagataat ttccaagcag tattggtttc gaatgccatg caagagatga agcgttttct
     7561 cgatgcagct gtacctaacg caaatcgcga cttaaacgaa agtgcctatt tgggagattt
     7621 gctcgttact gcttactcac aattcagtcg taaccgtatt ttcggacaaa tgattggaaa
     7681 aggctacacc gttaaatcgg cacaactcga gatgaacatg attgccgaag gtttttacgc
     7741 agttcattca ctcaacgaag tcaacaaaag cctcggagtt gatttgccaa tctgcgattt
     7801 cgtacaccga attctttacg aaggaggttc tccgtcaagg gaaattcggg tgttggaagc
     7861 gaaacttaaa taatgtgctg atgtgctagt atgctgatgt gctgattgct aacgcaaacc
     7921 ggaaaaaaag agcaacctat gcattgctaa agagcatgaa aatttcataa ccgtgggaga
     7981 acccgtggaa ataatgaatc tcttttcaac cgaacccggg aatagttcat cgctagcaac
     8041 ctcaattctt cataaatcta cgggaatagc ggtttttttc gtcggccgat gttccactga
     8101 cgatatagaa tcccgcgttt aggtgaccca ctgcaatatt ctgtacaaca ttgccgggat
     8161 tagcaataga ttgttcgtgc accaacctgc cttgaacatc gtagattttc agtattccga
     8221 tttcgcaatc gcggcaataa atgctgatat aactgctggc tggacttgga aaaacttcca
     8281 gtttgtaatg ttctccttcc gattccggaa tttgctcctg atatacatag gacacccacg
     8341 gccgggcttg tttaatgacc agtttatcat cactagtaat tttatattcg atatccatcg
     8401 cgaaagtgga attgccgact gccttgtata ggacttcaaa ctgatcatgt ataactgaaa
     8461 aataactgcg catttgttcc aggtattctt ccgtcataat aacgctgtct gcaggaacaa
     8521 ggttggaatg ctggatgata atgaaatcat tttcgctcat cgagacacgg tcgagcagaa
     8581 tttcttcggc aatcgtattg gcacccggat tcgtaatcaa atcatctccc acctgcgaat
     8641 taaggtagaa ggtattttca gaattgtaga ttggatcagt acttacgccc acgccattga
     8701 ctttttcatc ctcatggttt gggtgacata acacagccat cgacgctttg aaatgattga
     8761 tcctgtaaaa ttcacgctct tcaaaagcgc gcagattcca caaacttgca taaacctgct
     8821 tgatcgattt tgaaatatgt ccttcgtcgg gatgctgcgt tttggaatcg tacagtcctg
     8881 caccattaaa accgggcaaa tcttcattgt ttgtacttga tctgcatcga atggaagtgc
     8941 caacaggaaa tgaagtttgc atggctgata attccgtcag catccatggt ggcatttgcg
     9001 cgtcttctat ctgttccctg aaatcatcca gcaaatcatc gcggacatta cgatccgata
     9061 taaatgctgg attggccata atcgtttcga tctcttcgaa caaattatta taggacataa
     9121 actcctgata gaaataaaat ggcacaccaa atccatcagg aattgttcct tcaggaaatc
     9181 cgaatgtcct catggtcgcc acattcgcac actttgctcc aaagccatcg aacatattga
     9241 atgtgatttc actaagcggt aaaacagagg tgtaagtcaa gttaaggggt ggaatctgtt
     9301 ctgttagcgg acgaagattt tcatgccagg cattcacttc atctaaagtc gcttcgcgga
     9361 taaaatacct ttcctgttcg accttaaaat agatgtaatg gttcatcaac gcagcaatcg
     9421 aatcgattaa aagcggatcg cggataaatg cattgggaat gtgattttgg atagcccgga
     9481 gattgacatg cgacagcggt gtttgaatga cggacgttat aattccacca acacgcggta
     9541 aagtggttgg taatgattcg tacagtacga tgtccttagc accaggaact tcccccgttg
     9601 caagttgacg aaaaaagcca aatccttcgg ctggatttaa accccagtaa tttacatcgg
     9661 cgtatacatc gctttcgaga agcaccggaa cgcgcgacgc ttcaaagagc agactatctg
     9721 ctgcaaattg actttcgttc tgttcaataa cgaaatagga aagattattc cgtataaacg
     9781 gcatgttggc agcgagcatt tcgtggactt tctgcacctg actaaaatcc tcatagttgc
     9841 ctccgctaaa attgaacgca aatgttccaa gagtgccgtt gttggaaatt gtggaaggat
     9901 gataaatgat atgaccagta ttgatttcac tgctcacgaa atcaataccg atcgcatatg
     9961 caaaattttc gtgtagcgga tgagtgttcg tgttgatgaa atacaattta ggatgatcgg
    10021 tgtaaaaatc agcaatcatg tatttgacaa acacctttcc gttcaggtat tcattccagg
    10081 gaacaagttc gcgtgtgatt gataattcct tgaattttgt aaactgatcg actgcaacaa
    10141 gtccatcatt aatcaaggtc gattgtgctg cattcagtgg gctttggttg ggaatggcca
    10201 gtaattcaac tacatcattc ctaccgtcgt catccaaatc accggccgcc tgaattgaat
    10261 attgccgaac ttggtaattt tctagcggat aatttccaag cggttctgta atcacagtgc
    10321 taccaggttg ccccatagtg acagacgttg ctgtttcaaa cgcgccttcc ggagaatgtc
    10381 tcacttccaa tacataataa aacagtg
//
LOCUS       DAOOQW010000067        10323 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_8068_length_10323_cov_4.894819, whole genome shotgun sequence.
ACCESSION   DAOOQW010000067 DAOOQW010000000
VERSION     DAOOQW010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 10323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10323
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8068_length_10323_cov_4.894819"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(159..2132)
                     /gene="asnB"
                     /locus_tag="PK281_05620"
     CDS             complement(159..2132)
                     /gene="asnB"
                     /locus_tag="PK281_05620"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HOY48857.1"
                     /translation="MCGIAGILSPEGINPDDLYRMSQTIAHRGPDGDGFVFFSEQIAL
                     PRASKDTASTCIAQNLAWSPSDSNPQVPFRPDVGFMHRRLAIIDLEATGHQPMCSVDK
                     RYWITFNGEIYNYIELREELEKLGAVFITRSDTEVILQAYQYWGEACLHRFNGMWAFG
                     IYDTINQTFFASRDRFGVKPFYYHHTGKTLAFASEQKALLGLNWVPLEVNPDAVFDYF
                     VFSQIEYQAQGFLKGIDELPAGCLLHYQVKTGQCQIKTWYTLEVNHENETWSKSQHQL
                     HTETIRELLSDAVRLRLRADVPVGSCLSGGLDSSALVGWMRKHLGAEIPIHVYTAVFP
                     GNRVDEGHWADKMAQSVNATSHHVQPDSETLIRDLEDLTYCQDIPIWSTSTYAQYRVM
                     QLVKESGIKVVLDGQGGDELFGGYEPHRSFFWQGLSVMDMIRDMQQGMNLRSGLKFHA
                     HQQARYDLAFRMPVSLSSRFYQSYFQENSYLDKDFFNQHIQRFALQRQYRTDDLNGRL
                     ALEMQNTTLKGYLKCEDRCAMWHGIESRTPFADDLPLIEYLFSVPAVYKMHGSRLKAL
                     LRDASTSVLPEAIKNRTDKQGYTTPNNEWIKKIAPHFQEQFNQVLAPFLQVDKIKNEY
                     SNLFNPTGDADTGRVFKFISFAVWMQKLAQLKA"
     gene            complement(2135..2944)
                     /locus_tag="PK281_05625"
     CDS             complement(2135..2944)
                     /locus_tag="PK281_05625"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOY48858.1"
                     /translation="MSSITRKLSKLSRGLALLIKNPALLNRLIDEEDLHETAVKQEFK
                     LPDGLAEVSFQELWTSETEVVSPFAFLDGGSLPTDLALLRILAKKINAQTYFEIGTWR
                     GESVSNVADIIPTCYTLNLSAEQMRARQWEESYIALHGHYSQAFPNIQHLHGDSRSFD
                     FSSFEGKMDLVFVDGDHHYDSVVQDSQTAFRLVSPTGIIVWHDYGNSPEQVRWNVAHA
                     ILEGCPPEKRQQLCAISNTLCAAYLPFEVKRGVRHYPRKPEKSFRITLARS"
     gene            complement(2948..4207)
                     /locus_tag="PK281_05630"
     CDS             complement(2948..4207)
                     /locus_tag="PK281_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48859.1"
                     /translation="MLRLMLFTFVSRLATAAVNFGTIILLSRKLGPEGKGIATLILVI
                     VTSVQLICDFMGGAAMVYLSPRYRLKNLLFPAWTWTAAWSLLAPLVVWWIRPDLFEFY
                     GWHIAGLSFLNASMNQQLHLLNGREQFKLVNILNFLTAILTVSTLAAYIWWNPKPIHY
                     MYALYSGWLPAWIISLILLLRLPKVGENLSFKKSLQALLKFSSANQFGHMLQFSNQRI
                     AYFLLPAFALGIYSNAVSLAEAMWMLATSIATIQYGTIANSNDKAKAIELTVPLFRAS
                     ILVTSAAGLFICLLPASVFGLLFGDAFLPVKENLYFLMPGVVFISGYLILGHYFSGTG
                     QFMKNNYAITAGLLVTLIGFLFLAATQKQGINEHQAALVTTFANMATFFSVIWLFKSD
                     SGVALRELLPRFSDLGLLIQKLRSKVR"
     gene            complement(4201..5382)
                     /locus_tag="PK281_05635"
     CDS             complement(4201..5382)
                     /locus_tag="PK281_05635"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOY48860.1"
                     /translation="MSGEKPIHVLFLASWYPSKKEPTLGIFIRRHAQAVAIQAKVTVL
                     HAFADPELKEGEFRLDRKSEGNLHEFIVSYGRSVGGFSLFRKWKNWRQIRKHYQFGLE
                     KVIDWNGKPDLIHLHVPWPLGKIARLFSKKLKVPYGVTEHWTGYQPEDGRYTGSLLKR
                     VTRKTIRKAAFVAPVSHQLQVAMQNKGLKGNYVVVPNVVDTQVFVPGKPVDDKIRLVH
                     VSSLDDAQKDVTGLLKAFQSALREAPNLELQIIGGGNDESALKRLSNELGLTFRGVDF
                     KGKLQGEELVQELQKAHALILNSRYENQPVVILEALACGIPVISPAIGGIPEIIDESR
                     GILFNREDEFGLKKAILEFRSRYTSYNSTELRTYAVTNFSYQAVSNQLLALYTKIIAA
                     C"
     gene            complement(5406..>6769)
                     /locus_tag="PK281_05640"
     CDS             complement(5406..>6769)
                     /locus_tag="PK281_05640"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOY48861.1"
                     /translation="EDGSGFVNEALITGESMPVEKLPGSSVTGGTINEKGIFRMRAMK
                     TGKESVLAGIIDLVKSAQSKKPPVQRLGDQISAVFVPVVIAIALITWLVTYVALENTM
                     QESLLRAIAVLVISCPCAMGLATPTAVMAGIGRAAKAGILFRDGAAIEHLAKVKTIVL
                     DKTGTLTTGEFTIEKLDLFNQVDEEYVRSIIRSLESHSSHPIARSLVKYLGTGPLITF
                     NDIEETKGLGMKALTADLQEFELGSGRLIDDKELANKYDLFLLQNSTCIAAIRLADEL
                     KPGCKESIEALHALGIKTVMLSGDSDRKCQDVAKLTGIQEVYSSQSPEDKLQIIGKLN
                     KEAPTAMIGDGVNDAPALNLASVGISLTEASKVALGSAQVILLQKRSLDGLPLAVKLS
                     RSTLRTIHQNLFWAFFYNTIAIPVAAAGFLQPMIAAFSMAFSDVFVIGNSVLLQYRRL
                     NNS"
     assembly_gap    6770..6869
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6870..7693)
                     /locus_tag="PK281_05645"
     CDS             complement(<6870..7693)
                     /locus_tag="PK281_05645"
                     /inference="COORDINATES: protein motif:HMM:NF012350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HOY48862.1"
                     /translation="MKTAEAQRTLQVDGMTCANCALGIQNLLRNKGLKDAEVTFAIGE
                     VRYTSIDSYEESAIKKDIENLGYQIIEPETPVQTGLNSTEKRFIISLIFSLPLLAHMV
                     ISWHVLHMPWVQFGLSLPVMFIGWQYFGKSAWNSLKTGVPNMDVLITIGSGSAFIYSI
                     WGSIIGTGTANPEQYLFFETAASIITLVLLGNVIEQRSVKQTGNAIRELAALQPETAR
                     RISEHDNHTHIEEIPARLIVPGDLLLVNYGDKIPADGILEDGSGFVNEALITGESMP"
     gene            complement(7696..9006)
                     /locus_tag="PK281_05650"
     CDS             complement(7696..9006)
                     /locus_tag="PK281_05650"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOY48863.1"
                     /translation="MLADLLVTLLLVVLNGFFVGAEFAIVKVRQSQIDLKVASGNSFA
                     KFAKHIVTNLDAYLSASQLGITLASLGLGWIGERVVANLVLTIFAKIGYTADPVTAHS
                     IAIPIAFTLITVLHIVLGEQVPKTAAIRNPMQATLFVALPMRIFYVVFRPFIWTLNGM
                     SNLTLRMFGLSSADHTDIHTEEELRMILTESEEGGAIKQSEHELIQNVFEFDDRMVKQ
                     IYVPRTRVVAIDIEDDVEKIIDQVVEEGYSRLPVYKDSIDNIIGIIHSKDLIRHIRLK
                     KPIDLNTLLRPAYYVPLGKFVNDLLRELQRQHIQMAIVTNEFGGTEGIVTMEDIIEEL
                     VGEIQDEYDEEKPAVEKKSETEFIVNATSSISDVNDWLPIALEEGTDYETVSGLVNKL
                     FDGIPAVNEKKEFGGYQITILKRFKHSVEQVKLEVIDRDSLTLD"
     gene            9292..9365
                     /locus_tag="PK281_05655"
     tRNA            9292..9365
                     /locus_tag="PK281_05655"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9326..9328,aa:His,seq:gtg)
     gene            complement(9398..10156)
                     /locus_tag="PK281_05660"
     CDS             complement(9398..10156)
                     /locus_tag="PK281_05660"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HOY48864.1"
                     /translation="MQPKKGIICILLSMISIAMFGQQNTFLEKQKTYARVKSAWKNCH
                     VELVSLLKKQHLEEKKLQIFLRAFKKEDELEVWGKNSDSKTFVHLRTYKICAKSGSLG
                     SKIQQGDGQVPEGVYTIDRFNPASSYYLSLGVSYPNKIDLARSAGKDPGGDIFIHGEC
                     VTIGCLPMTNEKIEEIYLLAVMAKSAQQSNIPVHFFPFRMGKAQLELALKTKEGKLWG
                     DFWKNLQLVYQYFEDNKAPGTWVKGEGISYKRNG"
BASE COUNT         3021 a         2161 c         2076 g         2965 t
ORIGIN      
        1 gctagcgctg gaagtcaaga tagcagctgc tgaaagtagt gcgtagactg tgtgtttcat
       61 gtgatgtttg tgttaaaaaa tcctcagaac gcaaatctaa cgataaatta atatgaatga
      121 cgtgcaatta acctgaagtc agacttaatg caaaaaaatt acgctttcaa ttgagcgagt
      181 ttctgcatcc aaacagcgaa actaataaac ttaaaaactc ttccggtatc agcatcacca
      241 gtaggattga agagattaga atactcgttt ttaattttat caacttgtag aaaaggagcg
      301 agaacctgat taaattgttc ctggaaatga ggggcaatct tcttgatcca ctcattattg
      361 ggagttgtat atccttgctt atcggttcta ttttttattg cttctggtaa taccgatgtc
      421 gaggcatctc tcaaaagtgc ttttaatcta gaaccatgca ttttataaac agccggaacc
      481 gaaaatagat actctatcaa tggtaaatca tcggcaaatg gtgttcgaga ttcgattcca
      541 tgccacatcg cacatcgatc ttcacatttc aaataacctt tcaatgttgt attctgcatc
      601 tcaagcgcta aacgaccatt taaatcatcg gtccgatatt gacgttgtaa tgcaaatcgc
      661 tggatatgct ggttaaaaaa atccttgtcg aggtatgagt tttcctggaa ataggattgg
      721 taaaaacggg aacttaaact caccggcatg cgaaaagcta aatcgtaacg tgcctgttgg
      781 tgtgcatgaa attttaaacc cgatcgcaaa ttcatccctt gctgcatatc gcgaatcatg
      841 tccattaccg aaagtccttg ccagaaaaat gaacgatgtg gctcatatcc tccaaataac
      901 tcatcacctc cttgtccgtc taatactacc ttaattccac tttccttcac caattgcatc
      961 actcgatatt gcgcgtaggt actagttgac caaatcggaa tatcctgaca atatgttaaa
     1021 tcctctaaat ctcgaattaa tgtctccgaa tcgggttgaa catgatgcga agtggcatta
     1081 accgattgag ccattttatc agcccaatgc ccttcatcta ctcgattacc agggaaaaca
     1141 gccgtatata catgtatggg gatttcggct cctaaatgtt tcctcatcca acctactaat
     1201 gccgaagaat cgagtccgcc acttaagcaa gagccaactg ggacatcagc acgtaatcgc
     1261 aaacgaacgg catcactgag taattcacga atggtttcgg tatgaagttg atgttgagat
     1321 ttactccaag tctcattttc atgatttact tcaagcgtat accaagtttt aatttgacat
     1381 tgacccgttt ttacttggta atgcaataaa catccggcag gtaattcatc aattcctttt
     1441 aaaaaacctt gggcttgata ttcgatttga ctaaacacaa aataatcgaa aacagcatcg
     1501 ggatttactt ctaaaggcac ccaattcaag ccgagtagcg ctttttgttc ggaagcaaat
     1561 gccagtgttt tacctgtgtg gtgatagtag aaaggcttta caccaaaccg atcgcgtgaa
     1621 gcaaaaaatg tttggttgat ggtatcgtaa attccaaaag cccacattcc attaaaccga
     1681 tgcaaacagg cttcacccca atattgatag gcttgaagta taacttctgt atcgctgcgc
     1741 gtgatgaaaa cggctcctag tttttctaat tcttctcgta attcgatgta attgtaaatt
     1801 tccccgttaa aggttatcca gtaacgttta tcaacgctac acatgggctg atgtccagtt
     1861 gcttctaaat cgataatggc taaacgtcga tgcatgaaac ctacatcggg gcgaaaagga
     1921 acttgaggat tggaatcact tgggctccag gctaaatttt gagcaataca ggtagaagca
     1981 gtgtccttgc tggctcgtgg tagggcaatc tgctcggaga aaaagacaaa accatcaccg
     2041 tcgggaccac gatgagcaat ggtttgactc attcggtata aatcgtccgg attaattcct
     2101 tcgggagata gaatgccagc tattccacac atgattagct acgggcaagt gttatgcgga
     2161 aacttttttc gggtttgcgc ggataatgtc gaacgccacg tttcacttca aatggaaggt
     2221 aagctgcaca aagcgtgttg gatatggcgc aaagttgttg gcgtttttcg ggtggacatc
     2281 cttccaaaat tgcatgagct acattccaac gtacttgttc gggcgaattt ccgtagtcgt
     2341 gccaaacgat aattccagta ggacttacta atcgaaatgc cgtttgcgaa tcctgaacta
     2401 cgctatcgta atgatgatca ccatcgacga aaactaaatc catcttccct tcaaacgagc
     2461 tgaaatcgaa acttcgcgaa tcaccgtgca ggtgttgaat attcggaaat gcttgagaat
     2521 agtgaccatg cagagctatg taggattctt cccattgacg ggcacgcatc tgttcggcac
     2581 tgagatttag tgtgtaacaa gttgggatga tatcggctac attactcacg ctttctcctc
     2641 gccaagtacc aatttcaaaa taagtttgag cattgatttt cttagctaga atgcgaagaa
     2701 gagcaagatc ggttggtaaa gatccgccat cgagaaatgc aaatggacta acaacttctg
     2761 tttcactcgt ccataattct tgaaaggaaa cttctgctaa accatcaggg agtttaaatt
     2821 cttgcttaac agctgtttcg tgcaaatctt cctcatcaat caagcgattt aataaagccg
     2881 gattctttat cagcagagct aatccgcgag ataatttaga aagttttcgg gtgatggagg
     2941 acatatttca gcggactttg ctgcgaagct tttggatgag taaacccaaa tcagaaaatc
     3001 gaggtaacaa ttcacgaaga gctacgccgc tgtcggattt aaagagccaa ataacggaaa
     3061 aaaatgtcgc catattggca aaggtggtga caagagcagc ctgatgttcg ttgatacctt
     3121 gtttttgagt tgcagctaaa aagaggaaac cgattagcgt tacaagcaaa ccggcagtaa
     3181 ttgcataatt gtttttcatg aattgtcccg tgcctgagaa ataatgtccc aaaatcaagt
     3241 atccactgat aaacaccacg ccaggcatga gaaaatagag attttccttc accggtaaaa
     3301 aggcatctcc gaacagcaaa ccgaacaccg aagctggaag cagacaaata aacaatccag
     3361 cagcagaagt cactaagatg cttgcccgaa aaagcggaac cgttaattct attgcttttg
     3421 ctttatcgtt tgaattggca attgtaccat attgaatagt tgcgattgaa gtcgctaaca
     3481 tccacatggc ctctgcaagc gacactgcat tggaataaat accaagcgca aaggcaggta
     3541 aaagaaaata ggcaattcgc tgattgctaa actgtaacat gtgaccaaat tgattggccg
     3601 aggagaattt gagaagtgct tgcagacttt ttttgaagga taaattttca cccactttag
     3661 gaagtcgaag tagaagaatg agtgaaatga tccaagctgg taaccaaccc gaataaagcg
     3721 catacatata atgaataggc tttggattcc accaaatata agctgcgagg gtgcttaccg
     3781 tgagaatggc tgttaggaaa ttgaggatat tgaccagctt aaactgctca cgaccattta
     3841 acaaatgcaa ttgttgattc atgctagcat tgaggaacga caaacctgca atgtgccagc
     3901 cataaaattc aaataaatcg ggacgaatcc accaaacgac aagcggggcg agaaggctcc
     3961 aagcagcagt ccatgtccag gcgggaaaca gcagattttt cagccgatag cggggagaaa
     4021 ggtagaccat tgcggcaccg cccatgaaat cgcaaatgag ctgaacactc gtaacaataa
     4081 caagtatgag tgtggcgata ccttttcctt caggacctaa tttgcgcgat aataggatga
     4141 ttgttccgaa gttgactgca gccgtggcaa gtcggctcac aaacgtgaac aacatcaatc
     4201 ttagcatgca gcaataatct tagtatagag tgccagaagt tgattcgaaa cagcttgata
     4261 gctgaaattc gttacggcat aggtacgaag ctcggttgaa ttatagctag tatatcgact
     4321 tctaaattcg aggatggctt tttttagtcc aaattcatcc tcccgattaa ataagattcc
     4381 tctgctttca tctattattt caggaatacc accgattgct ggtgagatca ccggaatgcc
     4441 acaagcaaga gcttccaata tcaccactgg ttggttttcg tagcgactgt tgagtattaa
     4501 agcatgcgct ttttgtaatt cctgaaccag ttcttcccct tgaagtttcc ccttaaaatc
     4561 taccccacga aatgttaatc ctaattcatt ggataaacgc tttaatgcag attcgtcgtt
     4621 gccaccgcca attatctgaa gttctaaatt tggtgcttca cgtaatgcag attgaaatgc
     4681 cttcaataaa ccggtcacat ctttctgagc atcatctaaa gatgaaacgt gtactaaacg
     4741 aattttatca tcaacaggtt ttccaggaac aaatacctga gtatccacca catttggaac
     4801 aacaacatag ttgcctttca agcctttgtt ttgcattgcc acttgaagtt gatgacttac
     4861 cggcgcgaca aatgcagctt tgcgaatcgt ttttcgagtt acgcgtttaa gaagtgagcc
     4921 agtatagcga ccatcttcgg gttgataacc tgtccaatgt tctgtaacac cgtatggaac
     4981 tttcagtttt ttactaaata aacgagcaat tttgccaagt ggccaaggaa catgaagatg
     5041 gattaaatca ggttttccat tccaatcaat aactttttct agtccgaatt gatagtgttt
     5101 gcggatttgt cgccaatttt tccatttccg aaatagactg aaaccaccta cactgcgacc
     5161 ataagaaacg atgaattcat gtaggtttcc ttccgattta cgatcaagac gaaattcgcc
     5221 ttcttttaat tcgggatcag caaaagcgtg taatacagtc actttggctt gtatggcaac
     5281 agcctgagca tgtcggcgaa tgaagatacc gagcgtgggt tctttcttac tgggatacca
     5341 ggaagctagg aaaaggacgt gtatgggttt ttctccgctc acagcataca aattcgtcgc
     5401 tcaaattacg aattattcaa ccggcgatac tgaagcaaaa cggaattacc aataacaaaa
     5461 acatccgaga aagccatcga aaatgcggca atcatgggtt gaaggaatcc tgcagcagcg
     5521 accgggatcg cgatagtgtt gtagaaaaat gcccaaaaga gattttggtg tatcgtacgt
     5581 aaggtgcttc tactaagttt cacagctaat gggagtccat cgagcgagcg tttttgtagc
     5641 aaaataactt gagccgaacc aagggcaact ttactggctt cggttagtga aatacctaca
     5701 gaagctagat ttaatgcagg tgcatcattc acaccatctc caatcatagc agttggtgct
     5761 tctttgttca atttgccaat tatttgaagc ttatcctcgg gtgattgaga actgtagact
     5821 tcctgaatac ccgtaagttt cgcaacatcc tgacattttc ggtcgctatc tccactgagc
     5881 atgacagttt taattcctaa agcatgcaaa gcctcgatcg attctttgca tccaggtttg
     5941 agttcatctg ctaaacgaat agccgcaatg caggttgaat tttgtaaaag aaataagtcg
     6001 tatttattgg ctagttcttt atcatcaatc aatcgccctg aacctaattc aaattcttga
     6061 aggtcggctg ttaaggcttt cattccaagt cctttggttt cttcaatatc attgaatgtg
     6121 atgagcggac ctgttcccaa gtatttaacg agcgagcgag cgatagggtg ggatgaatgc
     6181 gattcaagtg aacgtattat agaacgtaca tattcctcat cgacctgatt aaacagatca
     6241 agtttttcga ttgtaaattc tcccgtagta agtgtgccgg ttttatcgag cacaatggtt
     6301 ttaacttttg ctaaatgctc aattgctgca ccgtctcgaa atagaattcc agcttttgca
     6361 gcccgtccaa ttcctgccat gacagctgtt ggagtagcaa gtcccattgc acatggacaa
     6421 gaaataacta aaacggctat tgctctgagc agactttcct gcatggtgtt ttcaagtgct
     6481 acataagtaa ccaaccaggt aatgagtgca attgcaataa caactgggac aaatactgca
     6541 cttatttgat cacctaaacg ttgaaccgga ggttttttgg attgggcaga tttaacgaga
     6601 tcgatgattc cggccagcac gctttctttt ccagttttca tggcgcgcat gcggaatatg
     6661 cctttttcgt tgattgttcc tccagttacg gaagatccag gaagtttctc aacaggcata
     6721 ctttcaccgg tgatgagtgc ttcgtttaca aaaccactcc catcttctan nnnnnnnnnn
     6781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnng gcatactttc accggtgatg agtgcttcgt
     6901 ttacaaaacc actcccatct tctaaaattc catccgctgg gattttatcg ccataattca
     6961 ccaagagcaa atcgccagga acgattaatc gagccggaat ttcttcaatg tgcgtatgat
     7021 tatcgtgttc gctaattcta cgagcagttt cgggttgaag agcagctagt tctcgaatag
     7081 catttccggt ttgcttaacg gagcgttgtt ctataacgtt tcctaagaga actagagtaa
     7141 tgatacttgc agcagtttcg aagaacaggt actgttctgg attggccgtt cctgttccaa
     7201 tgatacttcc ccaaatacta taaataaaag ctgaacctga gccaatagta atgagaacat
     7261 ccatattagg aactcctgtc tttaaggaat tccaggcact ttttccaaaa tactgccagc
     7321 caataaacat gactggtaaa ctcaaaccaa actgcaccca aggcatatgt aacacatgcc
     7381 aagaaatgac catatgagca agtaatggaa gcgaaaagat tagtgaaatg atgaatctct
     7441 tctcggttga gttcaatcct gtttgaacag gagtttcggg ttcaatgatt tgatacccta
     7501 aattctcaat gtctttcttt atggctgatt cttcgtacga atcaatacta gtgtagcgaa
     7561 cttcaccaat tgcaaaagtt acttcagcat ccttcagacc tttgtttctt aacaaattct
     7621 gaattcccaa ggcacagttg gcgcatgtca taccatccac ttggagcgta cgttgtgcct
     7681 ctgccgtttt cataatcagt ctaaagttaa cgaatctcgg tcaataacct ccagtttaac
     7741 ttgctcaaca ctatgtttaa agcgcttgag aattgtaatc tgataaccac cgaattcttt
     7801 tttctcgttt acagcaggaa tgccatcaaa taacttattg actaatcctg aaacagtctc
     7861 gtaatcagtt ccttcttcta gcgcaatagg taaccaatcg tttacatcac ttatcgaaga
     7921 agtcgcatta acaataaact ccgtttcgct tttcttttct accgctggtt tttcttcatc
     7981 atactcatcc tgaatctcac caaccaattc ctcaataata tcttccatgg ttacaattcc
     8041 ttcggttcca ccaaattcat ttgtaacaat cgccatttgg atatgttgac gttgaagttc
     8101 gcgcaaaaga tcattcacaa atttacctag cggaacatag taagctggac gaagtaaagt
     8161 gttaaggtcg ataggttttt tgaggcgaat gtgtctgatt agatctttcg agtggataat
     8221 tccgatgatg ttatcaatcg agtctttgta aacgggcaaa cgagaataac cttcttcaac
     8281 tacttggtcg atgatttttt ctacatcatc ttcaatatcg attgcaacca cacgcgtacg
     8341 gggaacataa atctgtttca ccatgcgatc gtcgaactcg aacacgtttt gaatgagttc
     8401 atgttccgat tgtttgatcg ctccaccttc ctcactttcg gtaagaatca ttcgaagttc
     8461 ttcttcggta tgaatatcgg tatgatcagc agaggataat ccgaacatac gtaatgtcaa
     8521 attcgacata ccgttcaaag tccaaatgaa cggacgaaac acaacgtaga aaatgcgcat
     8581 cggcaaggct acaaacaagg ttgcttgcat tggattgcgg atagcagcgg ttttcggaac
     8641 ctgttcaccc aaaactatat gtaatactgt aatgagtgtg aatgcaattg gaatagcaat
     8701 ggaatgagca gttacaggat cggctgtgta gccaatttta gcgaaaatgg tcaaaactaa
     8761 attggcaaca actcgttcac ctatccaacc caaaccaaga ctggcaagag taattcctaa
     8821 ctgagaggca ctcaggtaag catcgaggtt cgtaacgata tgtttagcaa acttggcgaa
     8881 actatttcca gaagctactt tcaggtcgat ttgtgattga cgcaccttca cgatggcaaa
     8941 ttcagctcct acaaagaagc cgtttaacac aaccaataga agcgtcacga gtagatcagc
     9001 taacataggt caagttaaat aatgaaacag gaagggagat tgtaaaacac aggaaagtgt
     9061 tcatccgtgt agtaaatacc tgaggtattg gtgtgcggtt aattcgagca aagccgcaca
     9121 atcgactttt agggggaggt tggtccaacg ttttttctta atggtgcaaa ggtataaaaa
     9181 taggcatctg aacaacaaat cactattcag ggccttttaa agcttttaga tatttttttt
     9241 caagccccct tgttggatgc attcctttat tatatttgcg ccctcttaat ggtggccata
     9301 gctcagttgg ttagagcgct agtttgtggc actagaggtc gggggttcga gaccccttgg
     9361 ccacccagaa accccgcttg tcggggtttt tttgtttcta cccatttcgt ttatagctaa
     9421 ttccttcacc ttttacccaa gtgccgggag ctttattgtc ttcgaaatat tgatatacga
     9481 gctgtaaatt cttccagaaa tctccccata atttgccttc tttcgttttt aaggctaatt
     9541 ctaattgcgc ttttcccatg cgaaaaggga agaaatgaac aggaatattg ctttgttgag
     9601 ccgactttgc catcacagcc aacaagtata tttcctcaat tttctcattg gtcatcggaa
     9661 ggcaaccaat tgtcacgcat tcaccatgaa taaatatatc cccgccagga tctttgcctg
     9721 cacttctagc taggtcgatt ttattgggat aagatacacc taaagacaaa taataggaag
     9781 aagctggatt gaaacgatcg atggtataaa ctccttcggg tacttgtcca tcgccttgtt
     9841 gaattttcga gcccaaactt cccgacttag cacatatctt atatgttctc agatgaacaa
     9901 aagtttttga gtcggaattt tttccccaca cttctaattc gtcctctttt ttgaacgctc
     9961 gaaggaagat ttgcagcttt ttttcttcga gatgttgttt ttttaagagt gaaacgagct
    10021 caacatggca atttttccat gccgatttta cccgagcata ggttttttgc ttttctaaaa
    10081 aggtgttttg ttgtccaaac atagcaatgg aaatcatgct taagaggatg catattatac
    10141 cttttttagg ctgcataagt cttgatttat tttttgagtg aacgttcttt tcggtcgtaa
    10201 agtattcata atcatcactt ccggaaaaag aaaaaccccg atcgaattga ccggggtttg
    10261 ctggtggtgc ctccagggat cgaaccaggg acacatggat tttcagtcca ttgctctacc
    10321 atc
//
LOCUS       DAOOQW010000068        10292 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_8107_length_10292_cov_5.080199, whole genome shotgun sequence.
ACCESSION   DAOOQW010000068 DAOOQW010000000
VERSION     DAOOQW010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 10292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10292
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8107_length_10292_cov_5.080199"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(12..656)
                     /locus_tag="PK281_05665"
     CDS             complement(12..656)
                     /locus_tag="PK281_05665"
                     /inference="COORDINATES: protein motif:HMM:NF012363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOY48865.1"
                     /translation="MTERKYLSCLVIFIIGCIGILNANAQVNNTSTSIPLDTTYTVWS
                     TKKKLIRDYPYIKPVEPSSSNAIIQKENVVYLTLKDTPFGERDLHVDIFIPNKKKKYY
                     PALVLVHGGGWRAGNKTLNTPMAQKLAENGFVVVSVEYRLSLEAKYPAAVHDIKAAIR
                     WMREHASEYQIDTKHIAIGGSSAGGQLASLIGSTNRNSKFEGVLGNLNSDWLNQ"
     gene            complement(660..2204)
                     /locus_tag="PK281_05670"
     CDS             complement(660..2204)
                     /locus_tag="PK281_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008611335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HOY48866.1"
                     /translation="MKTSMNIILFLTFFFVSSCKSNDDPTPPSGGDKPIQIVEDAFAF
                     SGAEGFGREATGGRGGKVMYVTNLNDAGTGSLRSAVQASGARYILFKISGTITLKSNL
                     NITNGNVTIAGQTAPGDGITLRDYPVNVNADNVIIRFLRFRMGDAAQQEGDALGGRFH
                     KNIMVDHCSMSWSTDECVSFYANENTTVQWCIIAESLRNSVHGKGAHGYGGIWGGKKA
                     SFHHNLLAHHDSRNARLGEEAGKAFALTDLVDLRNNVIYNWGSNSTYGGEGMNVNIVN
                     CYFKPGPATIANNGSSKQARIASFDKNKIKGTEVYDTWGKFYVDGNYIEGNANATNDN
                     WTYGVLNQFHSTYTTYPASCTDVNGNTIACEYTIPISDADKTAMNRTTPHDIENNVTT
                     HTALKAYDRVLDHAGASLVRDAVDVRIVTDVKNKTYQHEGSNGSTNGIIDTQGDVGGW
                     PELNSTTPPDDTDGDGMPDAWETTMKLDPAVAKPNGKDLSTGYDNIEVYLNSLVKAIV
                     EEQLKL"
     gene            complement(2279..4036)
                     /locus_tag="PK281_05675"
     CDS             complement(2279..4036)
                     /locus_tag="PK281_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4957 domain-containing protein"
                     /protein_id="HOY48867.1"
                     /translation="MALEEIKSVFKSIILLSERLDINSKQMTTKKFNIGGVLFIITLC
                     AIFFYSCDEEKIYPETRLFRPVLNQDLQAVGNTIVVNMGNLKKAVSYTVEVSRDTFKT
                     IDYTIQSDTSQFVINDELLNGDPLFWATLYQLRAVAHAEDPQYDSKVSDLGNVKTQQF
                     PTILNTPKSYDVIDVAARVSWLVAGAKVTGIKVFAGNDLKLTTPLLEFVVPDADQESG
                     ETFVVQGLDPATKYQVAIYSEDELRGWVDYQTLVQDIDPAASGVHDLTGATDPTAVAT
                     EVAAAADGDVILIARGSKFDMPTVALNKSITIRAAYGFQPKKAMLYNTNGNWNIAGGS
                     TIDHIRFVDLELRGGNFSGTYVFNPATDNISVNEITFENCYINTMRGIMRIRNNNVVI
                     ENYNIINTVVDSIGSYGLLTTDTNVNGPPSTTAQVHNILFQNSTFNHLQFGLSTYNNM
                     QSITIESYTFSNMVTTESGNYIFRFRGGAGNNDVINGISIKNSIFGPGWNISGTATNN
                     IRGKDGLPNTTIEVINTYGTSDWAFIATYEITGLPSSTYLGTQTALWVDPAKSNFNFK
                     DTGFAGKYNTGDPRWRIKL"
     gene            complement(4017..5873)
                     /locus_tag="PK281_05680"
     CDS             complement(4017..5873)
                     /locus_tag="PK281_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008611337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOY48868.1"
                     /translation="MKLRNILILMGMLVATSCNDFLDPKSLSTFDDTYIFSNVDDARK
                     GVNAAYTYFGQDAFRSRLSNNMTGNTDIERQSGWSSSSDRYQIWDLNALESNNDLKIV
                     WENAYKAIRDSNIAIEGIEKSSALNSSDGSVSKTMYNLLGEAYTIRAYWYSMLIFYFG
                     DVPFVQLAPKAGNDFYLPKEDRNVILSAVIDDLIAIESKMSWADELPYGIEQINREYT
                     LGMIARLALQRGGWFLTPDLTMDRETDYLEYYQIAKDYSAKLMLLKDRPLPADFRQVF
                     MNENKFIAPVNQDILFEVPFALGNGDVGWNIGITVEGGATANHDYGAGNNYMSMPPTY
                     YLSFDSDDIRRDVTCGMYKVNMNFEEEFIGYSNGLNTSNGHNISQGKWSRYFLETPPG
                     KASAKGTGINWPMLRYSDVLLMFAEAENELNGPTGEAQNAMRRVRQRAFDPSVWSDKV
                     DAYISNVSASKNDFFEAIVDERAWEFGGEMIRKYELIRWGNYSVKMDETVNGLKALND
                     ILFPGGTADPGAAAANNLPDFIYWKRNASGKFIILNPDSYVHPADVDGTWTQSPFLNV
                     LHDNTLTYPEWVTKDWANYVDRPNGVRYIFPIPASEIAQGVLKNDGYGFGGN"
     gene            complement(5902..9222)
                     /locus_tag="PK281_05685"
     CDS             complement(5902..9222)
                     /locus_tag="PK281_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008990180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOY48869.1"
                     /translation="MNKVLRLKWMIGALIFLIASIAFPVTSRAQEKKISGTIKSDDGT
                     SLPGVSVLEKGTKNGTVTDADGKFTLAVSSDATLVLSFIGMKPQEIVVGNQTSIEVTM
                     ESDLTALDEVVVVDYGYGTVKKSDMTGATASIGAKEIAKIPVASTAQALTGRLPGVTV
                     TTTDGSPDADILLRVRGGGSITQNNSPLLVVDGFIVSSIRDIPPTDVESITVLKDAAS
                     SAIYGARASNGVIVITTKKAAAGKTEVSYNGFVQFKNLPNDRKYQVLSPYEFALANYE
                     YAKLRSESDVEAFEKYFGVYDDLDIYKDKKPTDWQDELFGGTKVSQYHNINFKGGTDK
                     TKVMLSLTNNKDQGLMINSDFTRNVLNFKIDHQIGRTLKFEASTRITNTVVNGAGTAT
                     GTSTNPQVNVKDAVQTRPVNGIADELEPDPNQGAGSDDDYQKFITARIGPVKLAEQDW
                     REKTTTNYVLNAGLTWNVLKNLDVKSVFTSEQGYGKNLRFYGPDTGESFNNGGSAPLG
                     EQTKNNTTSYRWYNTIAYRVKDQGQHKLDFLVGQEISSTGGSEDFIRSEDFRNSITPD
                     ELFANMQLGRTDRIYTRNYIETNRFSLFGRGNYMFNDKYIATVTFRSDVSSIFAKGNR
                     VGYFPALALGWKMSQENFMQNIKFVNDLKMRVSYGTTGNDGVNAGAAQNLFAASTLRG
                     PGWGNVDNVYYTPNSTVLYNPDLVWERTIQRNLGFDFTVANSRISGTLDVYWNTANDL
                     LLTSAIPSNTGYTTQWNNIGTTSNKGVELGLTGQIIETSDFSLSVNVNGAINRGEIEK
                     LDGTNDRFVQSNWASTDLNNINDYYLQVGGKIGDIYGYETAGYYSVDDFESYDETTSK
                     YILKTDVASSGAVVGNTNIRPGFLKLVDQLTVDTDGDGKMDAGDGIIDAKDRKVIGNT
                     SPKIEGGFGVNASYKGFDFSMFFNFRFGNDVYNAGKIQYNQFRRVTYGNLLNTMNSEE
                     RFTYIDVDGSYTGTPGEVVTDLQQLGEMNEGKSIWSHASHGIAGAVIHSWAVEDGSFL
                     RLNNLSIGYSLPRDLISKVGLSRFRIYATGSNLHVWTKYSGYDPEVSSYQNPLTPGLD
                     YSSMPRGRAYTIGVDLTF"
     gene            complement(9389..>10292)
                     /locus_tag="PK281_05690"
     CDS             complement(9389..>10292)
                     /locus_tag="PK281_05690"
                     /inference="COORDINATES: protein motif:HMM:NF027479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4861 family protein"
                     /protein_id="HOY48870.1"
                     /translation="PRNYTKRTQAELSHKVDGKFVNREYIGGTFKNVDYLRVPPEHKD
                     HSWFIRYEGPGWESDKVGYRFYLDQRNATDVFGKKGPEMVLQNVGLDGFDSYHEIQPW
                     GMDVMKVGKSLGVGSIGAFENGKTIRVEKTDSVTCRITENGVVYSSILTNYYGWKLGG
                     KKYDVQSTISIHAGTRLSHQILSVTNNPDTLCTGIVKDKLAKVFKSKGDAQHWGYIAT
                     YGNQSLNNDALGLVVFFNPANALGFKEDEFSEIIALKPNMGKLEYYFEGAWELEANGI
                     SNETEFLSYIQSMASQLSQPVSAR"
BASE COUNT         2962 a         2296 c         1806 g         3228 t
ORIGIN      
        1 acttgcatcg cttattggtt caaccaatcg gaatttaaat taccaagcac tccttcaaat
       61 tttgaattcc gattggttga accaataagc gatgcaagtt ggccacccgc agaacttcct
      121 cctatcgcaa tatgctttgt gtctatttga tattcactag catgttcacg catccatcga
      181 atagcggctt taatatcatg gacagccgct ggatattttg cttcaaggga aagtctgtat
      241 tcaactgaaa ccacaacgaa accattttcg gctagctttt gagccatggg tgtgtttaag
      301 gttttatttc ctgcacgcca gccaccacca tgtactaata ctaaagcagg ataatacttt
      361 ttctttttat tgggaatgaa tatatcaaca tgaagatcgc gctcaccaaa aggtgtgtcc
      421 ttaagcgtaa ggtagactac attctccttt tgaatgattg catttgatga agatggctca
      481 accggtttga tgtagggata atcccgaatt aatttcttct tcgtgctcca tactgtataa
      541 gtggtatcaa gtggaattga agttgaggta ttatttacct gcgcattagc atttaagata
      601 ccaatgcatc cgataataaa aatcactaag catgaaaggt atttcctctc tgtcattttt
      661 tataatttca attgttcttc cacgattgct ttcaccagac tattcaagta gacttcaata
      721 ttgtcatatc cggtactaag atccttgcca tttggtttcg ctacagccgg gtcaagcttc
      781 attgttgttt cccatgcatc aggcattcca tctccgtcag tatcatcagg aggtgttgta
      841 ctgttcagtt cgggccatcc acctacatca ccttgtgtat caatgattcc atttgtgctt
      901 ccgttagagc cttcatgttg atacgtttta ttttttacat cagttactat tcgaacatca
      961 acagcatcgc gaacgagtga agcacccgca tgatcaagaa cacggtcgta tgcttttagg
     1021 gcagtatgag tggttacatt attctctatg tcgtgtggcg tagttctatt cattgctgtc
     1081 ttatctgcat ccgaaattgg aatagtgtat tcacaggcaa tggtattacc atttacatcc
     1141 gtacaagaag cagggtaggt agtgtaggta ctatgaaatt gattgagcac tccataggtc
     1201 caattatcat ttgtagcatt tgcattacct tcaatataat ttccatcaac ataaaattta
     1261 ccccaggtat cataaacttc tgttcccttg attttatttt tgtcgaacga agcaatccgt
     1321 gcctgcttgg agcttccatt gttcgcaatg gttgcagggc caggtttaaa ataacaattc
     1381 acaatattta cgttcatgcc ttcgcctcca taggtactat tactacccca gttataaatc
     1441 acattgttac gcagatcgac aagatcggta agggcaaatg cctttccagc ttcttcacca
     1501 agtcgtgcat tgcgactatc atgatgagcc aaaagattat gatgaaaaga tgctttcttc
     1561 cctccccaaa ttccaccata tccatgagca cctttaccgt gaacagaatt acgaagactt
     1621 tcagcaataa tacaccactg caccgttgta ttttcgttgg cataaaaaga aacacattca
     1681 tccgttgacc agctcatcga acaatgatca accattatgt tcttatgaaa tcttccgcca
     1741 agtgcatcac cctcttgttg tgcagcatca cccattcgga aacgaaggaa ccggataatg
     1801 acattatcag cattcacatt cacaggataa tcgcgaagcg tgattccatc ccctggagcc
     1861 gtttgtccag cgatcgtaac atttccattc gtaatattca gatttgattt taaggtaatc
     1921 gttccggaaa ttttaaacag aatgtatcgt gctccagagg cctgaaccgc tgaacgtaaa
     1981 cttccggtac cagcatcatt cagatttgta acatacatta ccttaccacc acgtcctcct
     2041 gtcgcttctc ggccaaagcc ttctgctccg gaaaatgcaa aagcgtcttc tacgatttga
     2101 atcggtttat ctccacctga tggaggagtt ggatcatcat tgctcttaca gctgctaaca
     2161 aaaaaaaatg ttagaaataa gataatattc atactcgtct tcattggcaa gacaatttgt
     2221 atttctctaa aaatgattaa acaaaaagtg tgaccttgat ccggtcacac ttttaatcct
     2281 aaagctttat tctccagcgc ggatctcccg tattatattt cccggcaaaa ccggtgtctt
     2341 taaagttgaa gttgctcttg gctggatcaa cccacaatgc agtctgtgtt cccaaatagg
     2401 tagaagaagg aagacctgtt atttcatatg ttgctatgaa cgcccaatca gaagtaccat
     2461 aagtattaat cacttcaatc gttgtgtttg gtagtccatc ctttccacga atgttgtttg
     2521 ttgccgttcc gctaatattc catcccggac caaaaataga attcttaata gaaattccat
     2581 tgatcacatc gttattaccc gcaccacctc tgaatctgaa aatataattt ccactttcag
     2641 ttgttaccat attgctgaac gtataacttt caatggtaat ggactgcata ttattgtaag
     2701 tagacaatcc aaattgcaaa tgattaaagg tactattctg aaataaaata ttatgaacct
     2761 gagccgtagt ggatggcggt ccattaacat ttgtatcggt agttaacaat ccgtagctcc
     2821 caatactgtc taccacagtg ttaataatgt tataattctc tatcaccaca ttattgtttc
     2881 gtattcgcat gatacccctc atcgtgttga tatagcaatt ctcaaaggta atttcattca
     2941 cactaatatt atctgtcgca ggattaaaga cataagtacc tgaaaaattt ccaccccgaa
     3001 gttcaagatc aacaaaacga atatgatcta tggttgagcc cccggcaata ttccagtttc
     3061 cattggtatt gtataacatt gccttcttgg gttgaaatcc ataagccgcg cgaatggtga
     3121 tcgatttgtt caatgctaca gtcggcatat caaactttga tcctcttgca ataagaatta
     3181 catcaccgtc tgcagctgcc gccacttctg tagccacagc tgttggatca gttgcgccag
     3241 tcaaatcatg aacgcccgaa gcagcaggat caatatcctg aacaagtgtt tgataatcaa
     3301 cccaaccgcg gagttcatct tcactataaa tggctacctg atattttgtt gccgggtcca
     3361 gtccttgaac aacaaatgtt tcacctgatt cctgatctgc atctggcact acaaactcca
     3421 gtaatggggt tgttaatttt agatcgttgc ctgcaaatac ttttattcct gtcacttttg
     3481 ctccagctac taaccatgat acccttgcag ccacatcaat tacatcataa cttttcgggg
     3541 tattgagaat agttgggaat tgttgtgtct ttacatttcc aaggtcagat accttgctgt
     3601 catattgagg atcctcggca tgagccactg cacgcaattg atagagcgtt gcccagaaaa
     3661 gaggatctcc gttaagaagt tcatcgttga ttacaaattg acttgtgtct gactgaatag
     3721 tataatcaat cgtcttaaat gtatcacggc tcacttctac tgtgtaggaa actgctttct
     3781 ttagatttcc catgttaaca acaatcgtat taccgactgc ttgtagatcc tggttaagca
     3841 caggtctgaa tagacgcgtc tcagggtaga tcttttcctc atcgcatgaa tagaagaata
     3901 ttgcgcataa ggttattata aaaagaactc cacctatgtt aaatttcttt gttgtcatct
     3961 gtttagaatt tatatctaat cgttcgctta atagtatgat agatttgaat accgatttaa
     4021 tttcctccaa agccatatcc atcattcttt agcacgccct gtgcaatttc actggctggt
     4081 attggaaaaa tatatcgtac tccattgggt ctatcaacat aatttgccca gtcttttgtt
     4141 acccactccg gataggtaag cgtgttatcg tgcaagacat ttaagaaagg actttgagtc
     4201 caggttccgt caacatcagc agggtgaaca tagctgtcgg ggttgagaat gataaatttc
     4261 ccactggcat tccttttcca ataaataaaa tcaggtaagt tgttggcggc tgcagctccg
     4321 ggatcagcag ttccacccgg aaataatatg tcgttcaaag ctttaagacc atttacggtc
     4381 tcatccatct tcacgctata gttaccccaa cgaatcaatt catatttgcg aatcatttca
     4441 ccaccaaact cccaggcgcg ttcatctaca attgcttcga agaaatcgtt cttgcttgcc
     4501 gagacgttcg aaatataagc atcgacttta tcgctccata cggatggatc aaaggcacgc
     4561 tgacgtacac gcctcattgc attctgcgct tcgccagtcg gtccgtttaa ttcattctcc
     4621 gcttctgcga acattagtaa cacatcagaa taacgaagca taggccaatt aataccagtg
     4681 cctttagcag aagcttttcc aggaggagtt tccagaaaat aacgactcca ttttccttga
     4741 gaaatattat ggccgtttga tgtgttgagg ccattactgt agccaataaa ctcttcttcg
     4801 aaattcatat tgactttata cattccgcag gtaacatctc ttcggatgtc atcactatca
     4861 aatgaaaggt aatatgttgg tggcatcgac atataattat tgccagcacc ataatcatga
     4921 ttagcggttg cgccaccttc cacggtaata ccgatattcc atccaacatc gccattacct
     4981 aaagcgaatg gtacttcaaa aagaatatcc tgattaacgg gtgctataaa tttattttcg
     5041 ttcataaaca cctgtctgaa atcagcaggc aaaggccggt ctttcaataa cattaacttg
     5101 gctgagtagt cctttgcgat ttggtaatat tcaaggtaat cagtctcacg atccattgtt
     5161 aagtcgggtg tcagaaacca tcccccacgt tgtaaagcta aacgtgcaat catacctaat
     5221 gtatactcac gatttatctg ttcaatgcca tacggaagtt catccgccca ggacattttg
     5281 ctttcgatag caataagatc atcaatcaca gcggatagta ttacgttacg atcctccttt
     5341 ggcaagtaaa aatcattacc tgcttttggc gcgagctgaa caaaaggcac atcaccaaaa
     5401 taaaatatta acatgctata ccagtaggct cgaatagtgt acgcttctcc caataaatta
     5461 tacatagtct tggagactga tccatctgaa gaattaagtg cgctgctctt ttcgatacct
     5521 tcaatggcaa tgtttgaatc gcggatggcc ttgtaggcat tttcccaaac aatttttaaa
     5581 tcgttgtttg attcaagtgc attaagatcc catatctgat accgatcaga tgaactgctc
     5641 catccgctct ggcgttcgat atcggtatta cctgtcatgt tgttagaaag gcgagagcga
     5701 aatgcatctt gcccaaagta cgtgtacgct gcgttaacac cttttcgtgc atcatctaca
     5761 ttcgaaaaaa tgtaggtatc gtcaaaagtt gaaagtgact tggggtcaag aaaatcattg
     5821 caggatgtcg ctaccaacat tcccatcaat attaaaatat ttctaagttt cataaacttc
     5881 attttttaaa tatttaattg attaaaatgt gagatcaact ccaatggtgt aagcacgacc
     5941 tcgtggcata gaagagtaat ctaagcccgg tgtaagtgga ttttggtatg agcttacttc
     6001 aggatcgtat ccggaatatt tagtccagac gtgaagattg ctgccggttg cgtaaattct
     6061 aaatctggat aaaccaactt ttgaaattaa atctcttggt aatgaataac caatggagag
     6121 attattcaat cgcaagaatg atccatcttc tactgcccat gaatgaatta ctgcacccgc
     6181 aattccatga cttgcgtgtg accaaatcga tttaccttca ttcatttcac cgagttgttg
     6241 aagatctgtt acaacttcac caggagtacc agtatacgat ccgtccacat cgatgtaggt
     6301 aaaacgctcc tccgaattca tcgtattcag caagtttcca taggtaactc ttctaaactg
     6361 gttgtactga attttacctg cgttgtaaac atcattacca aatctgaaat tgaaaaacat
     6421 ggaaaagtca aatcctttgt acgatgcgtt tactccaaac ccaccttcaa ttttaggtga
     6481 cgtgttacct attactttcc gatctttcgc atcgataata ccatcgcctg catccatctt
     6541 accatcacca tccgtatcaa cggtaagttg atccaccaat tttaaaaagc cgggtctgat
     6601 attcgtattt ccaacaactg caccagaact tgcaacgtct gtttttaaaa tatatttact
     6661 ggttgtttca tcatacgatt cgaaatcatc gacactatag tatcctgcag tttcatatcc
     6721 atagatgtca ccaatctttc caccgacctg taaataataa tcgttgatgt tgttaagatc
     6781 tgtgcttgcc caatttgatt gaacaaagcg atcattggta ccatccaatt tctcgatctc
     6841 gcctctgttg atcgcaccgt taacatttac agataagcta aagtcactag tctctataat
     6901 ctgtccggtt aatcccaact ctacaccttt attggaagtt gttccaatat tattccattg
     6961 tgttgtgtat cctgtattag aaggaatcgc tgaggttaat aaaagatcat tcgcggtatt
     7021 ccagtataca tcaagtgttc cagatattct ggaatttgcc accgtaaaat caaatcccaa
     7081 attgcgttga atagttcttt cccacacaag atccggattg tataaaacag tgctattggg
     7141 tgtgtaataa acgttatcaa catttcccca acccggaccg cgtagcgttg aagcagcaaa
     7201 taaattctgt gcggctcctg cgtttacacc atcatttcct gtcgtaccat aacttacgcg
     7261 catcttcaaa tcattaacaa actttatgtt ttgcataaag ttttcttgag acatcttcca
     7321 tcccaacgcc aatgcaggga agtatcctac ccgattacct tttgcaaaga tgctggagac
     7381 gtccgatcga aatgtaacgg tagcgatgta cttatcatta aacatatagt ttcctcttcc
     7441 gaaaagtgaa aagcgatttg tttcgatata atttcgggta tagatacgat cagttcttcc
     7501 caattgcatg tttgcaaata attcatcggg tgttatagaa tttctgaaat cttcagatcg
     7561 gataaaatct tcagatcctc cggtagaaga tatttcctga cctacaagaa aatcaagctt
     7621 atgttgcccc tgatctttta cccgataggc aatggtgtta taccatcgat agctggtcgt
     7681 attatttttg gtctgctcac ccagtggagc actacctcca ttgttgaacg attcacccgt
     7741 gtcaggacca tagaatctta agtttttacc gtatccctgt tcgcttgtaa aaacgctttt
     7801 aacatcaagg ttcttcaata cattccaggt caaccctgca tttaatacat aattagtagt
     7861 ggtcttttct ctccaatcct gttccgccaa tttaacgggt ccaatcctcg cagtaataaa
     7921 tttctgataa tcatcatctg atcctgctcc ttgattagga tcaggttcca gctcatctgc
     7981 aataccatta accggtcttg tttgaacggc atccttaacg tttacctgtg gattcgtaga
     8041 agttcctgta gctgtacctg caccgttaac aaccgtattg gtaattctgg ttgaagcttc
     8101 aaattttagt gttctaccaa tttgatgatc aattttaaag ttcagaacat tccgggtgaa
     8161 gtccgaatta atcatcaatc cttggtcttt attattggtg agactcagca tcactttagt
     8221 tttatccgta ccccctttga aattaatgtt gtgatattga gatacctttg tgccaccaaa
     8281 caattcatct tgccaatcgg ttggtttctt gtctttataa atatctaaat catcataaac
     8341 accaaagtat ttttcgaagg cttctacatc cgattcagac ctaagctttg cgtattcata
     8401 gttagccaaa gcaaattcat acggagacaa tacctgatac tttcgatcat taggtaaatt
     8461 cttgaattgc acaaaaccat tgtaggaaac ttcggttttt cctgccgctg ccttttttgt
     8521 agtaattaca ataactccat tcgatgcacg ggcaccatat atagcagacg atgcagcatc
     8581 cttcaaaacg gtaatacttt ccacatctgt gggaggtata tcgcgaatgc ttgaaacaat
     8641 aaaaccatcg acaactaaaa gtggtgaatt attttgggtg attgaacctc ctccgcgcac
     8701 tctcaataaa atatcggcat ctggagatcc atctgtagtt gttacggtta ctccaggcaa
     8761 tctacccgta agtgcctgag ccgtacttgc cactgggatc ttggcaattt ctttcgcacc
     8821 aatagaagca gtcgcgccag tcatatccga tttcttcact gtgccatatc cataatcaac
     8881 cacaacaacc tcgtccaatg cggttaagtc agactccata gttacctcaa ttgaagtttg
     8941 attaccaaca acaatttcct gaggtttcat cccgatgaag gataagacaa gtgtcgcatc
     9001 agaagatact gctaatgtaa atttcccatc ggcatcggta accgtaccat tttttgttcc
     9061 cttctcaagc actgaaaccc caggtaaaga agtaccatca tccgatttaa tagttccaga
     9121 aatctttttt tcctgagcac gtgaagtgac cggaaaagct attgaagcaa tcaaaaagat
     9181 caaagctcca atcatccatt tgagtcgtaa tactttattc ataggctcat tattttttta
     9241 atatgatttt tttcgatctg cgcaatcgat tgcgcatatg gatgtaaaca taacaacatt
     9301 ttttaaatta ttcatagttt acaatgtatt tcaatcgatt taatatagag agcaaccgat
     9361 tccgctaaat ctcaacattc tgagctgttt aacgcgctga aactggttga gataattgtg
     9421 aggccataga ctgtatatag cttaaaaact ctgtttcgtt agaaattcca tttgcttcca
     9481 attcccaggc gccttcgaaa taatattcca gtttacccat atttggtttt aatgctatga
     9541 tttcactgaa ttcatcttcc ttaaatccta gcgcatttgc gggattgaag aaaacgacca
     9601 accccagtgc atcattattt aaactttgat ttccgtatgt agcgatatag ccccaatgct
     9661 gtgcatcgcc tttactttta aatacttttg caagtttgtc tttgacaata ccggtacaca
     9721 gtgtatccgg attattcgta actgacaaaa tttgatgcga caagcgtgtg ccagcatgaa
     9781 ttgaaatagt agattgaaca tcatacttct tacctcctaa tttccatcca taataatttg
     9841 ttagaataga agaatagaca acgccattct cggttatccg acaggtaacg ctgtcagtct
     9901 tttcaacgcg aatagtttta ccattttcaa aggcaccaat agatccaaca cctaaagact
     9961 tacctacctt cattacatcc ataccccacg gttgaatttc atggtaagaa tcaaatccat
    10021 ccagaccaac attttgcaat accatctcag gtcctttttt tccaaataca tcagtcgcgt
    10081 ttcgttgatc aagataaaag cgatatccta ctttatctga ttcccaaccc ggaccttcat
    10141 accggataaa ccatgagtga tccttatgct caggaggaac acggagataa tcaacattct
    10201 taaatgtgcc accgatgtat tcacgattaa caaacttgcc gtctacctta tgagacaatt
    10261 ctgcctgagt acgtttggta tagtttcttg gc
//
LOCUS       DAOOQW010000069        10193 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_8203_length_10193_cov_5.460347, whole genome shotgun sequence.
ACCESSION   DAOOQW010000069 DAOOQW010000000
VERSION     DAOOQW010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 10193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10193
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8203_length_10193_cov_5.460347"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2456)
                     /locus_tag="PK281_05695"
     CDS             complement(<1..2456)
                     /locus_tag="PK281_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48871.1"
                     /translation="MNLNFYKYFIGILLISLSGFVQAQNTPLNISIVVLPPYSMEAMD
                     YFSSPTQTTLTILNPTNQPLSVYLAGSLKNLSTNQSVTIPNNVTPAVPPLIVEPGVRI
                     MTGADLQQYVDESTVQFSGISQEQVISGNLPEGEYQLCLQAYDYQTHELRSQNEPLGC
                     SNVFTIQFVQPPILISPQCSTEVTSTTPQNIIFSWFPPAVSINPGLLNYEFKLVELPE
                     GMNPLQVMNSTGVPVLTHTTPSPVFLYSTQSPALIPGRFYAWRVKVTDVSQSIVFSNN
                     GESEICTFRYGMPFLFDTPPVSSLVYPVPGKRIPFIQVPLISRTEPFDSTVTSLSVET
                     ELREGNVMFDLIQQTKEYPNGLRAQLQQQLGVFPTIAQLQHAEIGRDLSNGATFGELK
                     PGKTYSVSSNHSFTYGDGHVETSNANGNFLQGFEMPVLQVPSRDTTYLNHTVSFQFKT
                     SDFPTFLPTEAGIIPPVDLLNSLIGNDPKLFQAEVKERYRIEVAKTSSFSTILFAATG
                     VFNETLQILKTTTPEIVKDRFYKSVKKDLFLPDTGEYYWRVHWLSNRTDSASTPYQTS
                     EVNRFRIAGTLAEDAIQSACMADCDAPLITDRTPVGGLLVNQKVKVGKFEMEIKTIEY
                     LGPAARGTGLIRVPFMNANIKVEFNEALFNGAKQFYQGNVFATHDNSGFLPNIPGVGQ
                     LTVTNQEELLAYVSDSRYKSVLDPSVPMGLPLGLDKEIEGERYMVAIVGMRFDPERAS
                     LSAAMSFALPFLPARTSDGETQRIGLGASDICFHPDGLAGAGVGTLYLADPLEADYAP
                     GQYFRLNNTRVTAGVVADSGT"
     gene            complement(2456..4120)
                     /locus_tag="PK281_05700"
     CDS             complement(2456..4120)
                     /locus_tag="PK281_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48872.1"
                     /translation="MRLLFLSICACLFAQFSVFSQDLESIGKQKPIKFGGGLSLFADA
                     YSSHGIPPRSTPFNWRLTGSPTVTIYGISFPFYFNIGSQERSFTQPFNQYGVSPRYKW
                     ITAHLGWRNLSYSQYSLAGLSFYGAALELNPGKFRFSAMYGRFNKAVREDTTQQFIQA
                     TPTYKRIGYSARIGVGTPQNFFDLIYFKAQDDSTSYKDHPQRLKPAENAVFGINSKFL
                     LFKHVQLNLDAAASAYTRNIQLDSISDPQFKKFASILNVNASSQLLYAGNASIGYVSK
                     YINLKLQYKRVSQDYKSMGAYMYQTDLESYTIEPSFNLFKNKVRINGSFGKQNDNLSG
                     KRLISNLRTIGSVGLSVNVSKTYSVDLQYGNYGIAQRAGIIPINDTIRMAIANQNINL
                     MNRFTLMNKKRAMTVILLTTYQEMTNLNPLFPAFVESKVMLGNLNVNYLVIPTGINFS
                     GGFNYTRSQFGAGTIVSVGPILGVGKTLFKSRLNTSINTSLLSNKFDGKANGTTITGG
                     LNLTLRINKSQSLLGNIRFTSNSSNNPLSVPFTEVFFSAGYQFNLH"
     gene            4371..5705
                     /locus_tag="PK281_05705"
     CDS             4371..5705
                     /locus_tag="PK281_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOY48873.1"
                     /translation="MATRKPKQKKIYVLDTSVILYNHNAIYSFEDNDVAIPITVLEEL
                     DNFKKGNDTKNFEAREFIREMDRLSGDFILTEWLTLGPKLGRFKVEMNGKGKLDAVQI
                     LGEDKADHRIINSALALSKENPEMKVVLVTKDINLRLKAKSLNLHSEDFQTGKIKNVE
                     ELYTGKSVIHGVNVAVIDQLYTHGSCSPSDFLSDDPIFNHFYILKNGKHSVLATYNPA
                     TLQMERVEKTAAYGIRPRNAEQIFALHALMNPEVKLVTLQGVAGTGKTLLALSGALEQ
                     KREYRQVYLARPIVPLSNKDIGYLPGDIKSKLNPYMEPLWDNLKMIQNQWEETDKEYK
                     KITEMVESEKLMITPLAYIRGRSLSNIYFIVDEAQNLTPHEVKTIITRAGENTKIVFT
                     GDIFQIDTPYLDTQSNGLSYLIDKIRNNPLYAHITLEKGERSELANLANDLL"
     gene            5946..6638
                     /locus_tag="PK281_05710"
     CDS             5946..6638
                     /locus_tag="PK281_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48874.1"
                     /translation="MKKTYITPALFAIILLVLGSCEKKFKETSPLNLKVKMINPDVSD
                     FVTLKTGQIYLSTFGITGDRKQGDDVLFVNNLPADSYADLKTETVSPAVNYSLPQGTY
                     TNLVVNMGIKPKDSIGAIVLQGEYVEEEEGGEHEIHRFIFRYDPETLYSLNTTLLGEI
                     NVVSDQNTSANVILDVKYLFANISEQMWHSSHHIEIDGVETILINETENTTIYNLLIS
                     RLNAAFSAQVVQ"
     gene            6644..7231
                     /locus_tag="PK281_05715"
     CDS             6644..7231
                     /locus_tag="PK281_05715"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOY48875.1"
                     /translation="MAEEASDKQLVDGCVRGERRFQELLYKRFSPKMYSVCLGYAGRG
                     NEAADMLQDAFIKVFRNIGEFRFDCPLEAWIRRIVVNTAIDQYRKASRQAMPLSLDDT
                     LELDDETVFSHPSDHKYFLDLVSALPDGYRLIFNLYVIEGLNHREIAEQVGISEGTSK
                     SQLARAKKYLQQKLKQETATNPSAQKNEGRSFQLV"
     gene            7203..8543
                     /locus_tag="PK281_05720"
     CDS             7203..8543
                     /locus_tag="PK281_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48876.1"
                     /translation="MKEDLFNWYKKGFDNLPPTEPPPMVWENIASALDGKPKRRIPAF
                     WWWSGLSILVVGLATAVWLNYSPTSNVANAKPESFSAQSKRPQTVMKHTAPSKQKESS
                     NSIQSKTVNSDNPFRETSPSQASTEQVATANNTSFSKQKASITSHSPEILVSTPSNPE
                     NNLENTNAPVNSLNSNPLAEETNLSITQKTETILTHSSGLILPKRSFIGFELSTINPE
                     LTTTSQQLPELIIPKMNNSGSSWFFGVASEINNNWVFNHDLVDAMRKESMNSILPHFS
                     STHSLVASKEFLPNHRIRAELILSKKEGQHYVTYLNGQLLKKDLHLNYSGATLLYDYR
                     NPSPKNLFGFTLRTHWLGGASLCYLSESKLAIGQTMVSPSGYRKMNISLVAGMEKEVF
                     FTRQLSLGAGLRLMGGLTNIFKGYENIPAWFNRTHTGNIAGTLSVRYYLNIPNH"
     gene            8723..>10193
                     /locus_tag="PK281_05725"
     CDS             8723..>10193
                     /locus_tag="PK281_05725"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOY48877.1"
                     /translation="MITHRLLQRLVLGFVFLFPHISISQAYWWKDAVFYEVFVRSFYD
                     SNGDGKGDINGLIEKLDYLNDGNPSTQSDLGVTALWLMPIMPSPSYHGYDVTDYYGVE
                     SDYGNRQAFKNLIDSAHARGIKVIIDLVMNHSSSSHPWFQSALGSVNSPFRDYYIWED
                     SNPGFTGPWGQTVWHPRNGKWNYGIFWNEMPDLNYRNTSVQTEMKSVARFWTDSMHVD
                     GFRLDAIQYLVEDGTQLSGTPETFAYIEDWSSNFKSVHPEVMTVGEIWSPTAVGVPYV
                     QDGRLDMCFEFDLAESIISSLLANSPNTFQNMMNTVNTSFPESRYGTFLTNHDINRSF
                     DQLGQNTDKAKLAAAVYLTLPGTPFIYYGEEIGMTGTGNDETKRKPMQWSNLANAGFS
                     WGTPWEPLASNYNLVNVAAQQQDNTSLWHTYQQFISVRKENTALRKGAYKVMADVNTE
                     LIGYARYTNDELLLVIHNFTGNDVSNAAFALGATNLPEGN"
BASE COUNT         3222 a         1973 c         2033 g         2965 t
ORIGIN      
        1 gtacccgaat cggcaactac accagctgtt acacgtgtat tatttaaacg gaaatattgt
       61 ccgggagcat aatctgcttc gagtggatca gctaaataga gtgtgccaac acccgctcca
      121 gccaaaccat cgggatgaaa gcaaatatcc gaagccccaa gcccaattcg ctgcgtttct
      181 ccatcggatg tacgagcagg taggaaagga agtgcaaaag acatagcagc agataaggaa
      241 gcacgctcgg gatcgaatct cattcccaca attgccacca tataacgttc tccttcaatc
      301 tctttgtcaa gtcccaaagg caatcccatc ggtacagaag gatctaaaac tgatttgtac
      361 ctgctatcgc tcacgtaagc aagcaattct tcttgattgg taacggtaag ctgtccaact
      421 ccgggaatat tgggcaggaa accactgttg tcgtgagtag caaatacatt tccttgatag
      481 aattgtttcg ctccattaaa gagtgcttca ttgaattcaa ccttgatgtt agcgttcata
      541 aatggcacac gaatcaagcc tgtacctcga gctgcaggac cgaggtattc gattgtttta
      601 atctccattt cgaactttcc aaccttcacc ttttgattca ctaataaacc accaacagga
      661 gttctatcgg taattaaagg cgcatcacaa tcggccatac aggcactttg aatggcatct
      721 tctgctaatg tcccagctat tcgaaagcga tttacttcac ttgtttgata aggtgtagat
      781 gcggagtcgg tgcggttact aagccagtgt actcgccagt aatactcacc tgtatcggga
      841 agaaatagat ccttttttac tgatttataa aatcgatctt ttacaatttc gggagttgtg
      901 gttttgagaa tttgaagtgt ttcattgaaa actcctgttg cggcaaataa aatcgtagag
      961 aaactgctcg tttttgccac ttcaattcga taccgctctt ttacttctgc ctgaaataat
     1021 ttggggtcat ttccgataag ggaattcaat aaatcaacag gaggaattat cccagcttct
     1081 gtaggtaaaa aagtggggaa atcggaggtt ttgaactgaa atgaaacggt gtgatttaga
     1141 tacgttgtat cacgcgaggg tacttgtaaa acaggcattt cgaacccttg aagaaaatta
     1201 ccattcgcat tggatgtttc tacatgtcca tctccatatg taaaagaatg attgctagaa
     1261 acagaatatg ttttacccgg cttaagctcg ccaaaagtgg ctccattact taaatcacga
     1321 ccgatttctg cgtgttgcaa ttgagcaata gtgggaaaaa ctccaagttg ctgttgtaat
     1381 tgagctctta atccgttggg atattctttt gtttgttgaa taagatcaaa catcacattt
     1441 ccttctcgta attctgtttc aaccgaaagg cttgtgactg ttgagtcaaa tggctccgtc
     1501 cgactaatta acggaacctg aataaaagga atacgcttcc ccggaacagg ataaaccaaa
     1561 gaggaaactg gaggagtatc aaaaagaaag ggcattccat aacgaaacgt acagatttcg
     1621 ctttcaccat tattactaaa tacaatcgac tgagacacat ccgttacttt cactctccaa
     1681 gcataaaaac gtcctggaat taatgctgga ctttgcgtcg agtacaaaaa caccggagat
     1741 ggagttgtat gcgttaaaac aggcactccg gtagaattca ttacctgtaa aggattcatt
     1801 ccttcgggca attcaaccaa tttaaactca tagttcaaca aacccggatt aatcgaaact
     1861 gcaggtggaa accaactaaa aataatattc tgaggagttg tactagtaac ctctgtagag
     1921 cattgtgggc ttattaaaat gggaggttga acaaactgaa tcgtgaatac attactacat
     1981 cctaatggtt cgttttgaga tcgcaattca tgtgtttgat aatcatacgc ttgaaggcac
     2041 aattgatact ctccttcggg tagatttcca ctaataactt gttcttgaga aatgcccgaa
     2101 aactgaacag ttgactcatc aacatattgc tgcaaatcgg caccagtcat aatacgaacg
     2161 cccggttcaa caatcaaagg aggaacagca ggagttacat tattgggaat tgtaacactt
     2221 tgattggtag ataaattttt cagacttcca gctaaataaa ccgaaagagg ttgattagtt
     2281 gggttaagga tagtgagtgt ggtttgcgtt ggactactga aataatccat tgcttccatc
     2341 gaataaggag gcaacacaac aatgctgatg ttcagtggtg tattttgagc ttgaacaaac
     2401 ccggaaaggg aaatgagcaa aatgcctatg aaatatttat agaaattcag gttcatcagt
     2461 gaagattaaa ttgatatccg gcagaaaaga aaacttcggt aaatggtaca gatagcggat
     2521 tgttagaaga attgcttgta aatcgaatat ttcccaataa actctgagat ttattaattc
     2581 gtaacgtgag atttaaacct cctgtaatgg ttgtcccatt tgcctttcca tcaaatttgt
     2641 tacttaataa acttgtattt attgacgtat ttaatcgtga tttaaatagc gtttttccaa
     2701 cacctaaaat gggtccgaca gagacaattg tccctgctcc aaactggctt cgggtataat
     2761 taaatccacc actgaaatta attcctgtcg ggatgactaa ataattaaca ttaagatttc
     2821 cgagcatgac tttgctctca acaaaagccg gaaacaatgg atttagattg gtcatttcct
     2881 gataagtagt gagcagaata actgtcatag ctcttttttt attcatgaga gtaaagcgat
     2941 tcatcagatt aatattctga ttcgcaatgg ccattcgaat ggtatcatta atgggaataa
     3001 ttccagcacg ttgagctatt ccataattgc catattgaag gtcaacactg tatgttttac
     3061 tcacattaac acttaatcca acagaaccta tagttcttag gttgctgatt aatcgcttgc
     3121 cagaaaggtt gtcgttctgc tttccaaaac ttccgtttat ccgtacttta ttcttaaata
     3181 aattaaagga tggctcgatt gtataagatt ctaaatcggt ttgatacatg taagcaccca
     3241 tagatttata atcttggcta actcgtttgt attgaagttt aagattgatg tactttgaaa
     3301 catatccaat acttgcattt ccggcatata gtaattgaga cgaagcgttt acattcagaa
     3361 tacttgcgaa tttcttgaat tgaggatcgc tgattgaatc gagttgtatg ttgcgtgtat
     3421 aggcacttgc agcagcgtca agattaagtt gaacatgctt gaataataaa aacttcgagt
     3481 taattccaaa tactgcgttt tcggcgggct tcagacgttg tggatgatct ttatatgagg
     3541 tagaatcgtc ttgtgcttta aaataaatta agtcgaaaaa gttctgtgga gttcctactc
     3601 caattcgggc actatagcct atacgtttgt atgttggcgt agcttggata aattgctgtg
     3661 ttgtgtcttc gcgaaccgct ttattaaatc gaccatacat ggcagaaaac cgaaattttc
     3721 cgggattcaa ttcgagtgct gcaccgtaaa atgataagcc tgctaaagaa tattgggaat
     3781 aagataaatt tctccaacct aagtgagctg taatccactt gtagcgagga cttacaccat
     3841 attgattaaa tggttgagta aagctgcgtt cttgagagcc gatattgaaa taaaatggaa
     3901 aactaattcc ataaatagta accgtaggac ttccggttaa tcgccagtta aatggagtgc
     3961 ttcgaggagg aatgccgtga gaagaatagg catctgcaaa cagagataat ccgccaccaa
     4021 acttaattgg cttttgttta ccgattgatt ctagatcttg tgaaaagacc gaaaactgag
     4081 cgaacaaaca agcacaaatc gacagaaata aaagacgcat ttacccagaa tcttggttgt
     4141 tccaaagcca ttccggactt tactgggtat caatcaccaa tattagtgag taaaaaatgt
     4201 tgggtgggta aaaaatgttg ttaaagcaat gtgactggca ctttaacaaa tctgaaataa
     4261 tgttggatga ttgatttcaa aacctgttga aacatcgttc acaacttcta cctcacctaa
     4321 taccgccccg ttttacttta cgtttagatt agcaaaaaac ggtttcttac atggctactc
     4381 gcaaacctaa acagaagaag atttatgttc tagatacatc ggtcattcta tacaatcaca
     4441 acgcgattta cagttttgag gataacgatg tagcgattcc aataacggtt ttagaagagc
     4501 tcgacaattt caaaaaaggg aacgacacca agaattttga ggcacgtgag tttattcgag
     4561 agatggatag gctttcggga gattttatcc tgacagaatg gttgacgctt ggtccgaaac
     4621 tagggcgttt caaagtagag atgaatggca aaggaaagct ggatgctgtt caaattttag
     4681 gggaagacaa ggccgatcat cggatcatta attcggcact tgctttaagc aaggaaaatc
     4741 ccgagatgaa ggttgttttg gtgaccaaag acatcaacct acgtttgaaa gctaaatcgc
     4801 tcaacttgca tagtgaggat tttcaaaccg gtaaaatcaa gaatgtagag gaattataca
     4861 cgggcaaatc ggtgattcat ggagtgaacg tggccgtgat tgatcagctg tatacacatg
     4921 gttcctgttc gccttcagat ttcttaagtg acgatccaat tttcaaccat ttctacatcc
     4981 tcaaaaacgg aaaacattcg gttttggcga catacaatcc agctacttta caaatggagc
     5041 gggttgaaaa aacggcagct tatggaattc gtccacgaaa tgccgaacag attttcgctt
     5101 tacatgcctt gatgaatccc gaagtgaaat tggtgacttt acaaggtgta gcaggaacag
     5161 gtaagacctt attagcatta agtggagcac ttgaacagaa acgcgaatat cgtcaggtgt
     5221 atcttgctcg tcccattgtg cctttgagca ataaggatat aggttattta ccgggtgata
     5281 taaaaagcaa gttgaatcct tatatggaac cactttggga caatctcaaa atgattcaaa
     5341 atcagtggga agagactgat aaagaataca agaaaatcac cgaaatggtg gagagcgaaa
     5401 agctgatgat aactccatta gcttatatac gcggaagaag tttatcgaat atttatttca
     5461 ttgtggatga ggctcaaaac ctaacaccac atgaagtaaa aaccattatc acccgtgctg
     5521 gagaaaacac taaaattgtc tttacaggtg atattttcca aatcgacaca ccttatcttg
     5581 atactcagtc gaatggatta agctatttga ttgacaagat tcgcaacaat cctctgtatg
     5641 ctcacataac actggaaaaa ggtgagcgtt ctgaattggc taatttggca aacgatcttt
     5701 tgtaatagct tttaaaaagg agcggaattc tcgtcttgat aaccgcttca gcattattta
     5761 aaacaataaa ggctttctta catttttcaa caaagaaatg taatcgtgaa tttttttatt
     5821 cgctggctaa gctattgata taaaacacat tagaacatta tttatattgc agaactcaaa
     5881 ctaaaagtat agacgtgcaa cgctcttttt aatttatacg tctaaaagac aggtacatag
     5941 atcctatgaa aaaaacttac atcacacctg ccttatttgc aattatactt cttgttttag
     6001 gctcttgcga aaagaagttt aaagaaacat ctcctttaaa cttgaaggtt aaaatgatca
     6061 atcccgatgt atcagatttt gtgacgctaa aaacgggaca aatttatcta agcacattcg
     6121 gaattactgg cgaccgtaaa caaggcgatg atgttctgtt tgtgaataat ctacctgccg
     6181 attcgtatgc agatcttaag actgaaacag tttccccggc tgtcaattat tctttaccac
     6241 aaggcacata tacgaatcta gtagtaaaca tgggaatcaa gcccaaggat tcaattggag
     6301 ccattgtatt gcaaggagaa tatgtggaag aagaagaagg tggcgaacat gaaattcatc
     6361 gttttatttt taggtatgat cccgaaacat tgtattcgct aaacaccaca ttattgggag
     6421 aaattaatgt ggtttcagat caaaacacga gtgcaaatgt gattttagat gttaagtatc
     6481 tgtttgctaa tatttcagag caaatgtggc attcttcaca tcatattgaa attgatggag
     6541 tagaaaccat tttgattaac gaaactgaaa acactaccat atacaacttg ttgattagcc
     6601 gactaaatgc cgcattttca gcgcaagtag ttcaataatt gctatggccg aagaagcctc
     6661 agataagcaa cttgttgatg gctgcgttcg tggagaacgt cgctttcagg aattgctata
     6721 caaacgcttc tcccctaaaa tgtactctgt ttgcctaggc tatgcaggac gaggcaatga
     6781 agcagcagat atgcttcaag atgcctttat caaagttttt agaaacattg gtgagttccg
     6841 atttgattgt cctctagaag cttggattag acgcattgtg gttaatacag ctatagatca
     6901 gtatcgaaaa gctagtcgac aagcaatgcc actgtcgttg gatgatacac ttgaattaga
     6961 cgacgaaacc gtattctctc atccaagtga tcataagtac tttcttgatt tagtatctgc
     7021 tctacccgat ggctatcgac tcattttcaa tctgtatgtg attgaaggat taaatcaccg
     7081 ggaaatagcc gaacaggttg gaattagcga aggaacatct aagtcgcaac tagctcgagc
     7141 taagaaatac ttacaacaaa aactcaagca ggaaactgcc acaaacccat cagctcaaaa
     7201 aaatgaagga agatcttttc aattggtata agaaaggttt tgataacctt ccgccaaccg
     7261 aacctccacc aatggtatgg gagaatattg cctctgcact cgatggtaaa cccaagcgta
     7321 gaattccagc attttggtgg tggagtggac taagtattct agttgttgga ttagcaacag
     7381 cagtatggtt gaactattct cctacaagca acgtcgcaaa cgcaaaacct gaatcattct
     7441 ccgctcaatc aaaacgaccg caaacagtga tgaagcatac agcgccttca aaacaaaaag
     7501 aatcctcaaa ttcgattcaa tcaaagactg ttaattctga taatccattt cgggaaacaa
     7561 gtcctagcca agcctcaacg gaacaagtcg ctaccgctaa taatacaagt ttttctaagc
     7621 agaaagcttc aataacaagt cattctcccg aaatacttgt ttccacacct agtaatcctg
     7681 aaaataatct agaaaatact aatgctccag ttaattcact caacagcaac ccactagctg
     7741 aagaaactaa tctatctatc acccaaaaaa cagagactat cctgactcat tcttctggat
     7801 taattttacc aaaacgatct ttcataggct ttgagctttc cacaataaac ccagaattaa
     7861 caaccacttc acagcagtta cccgaattaa tcatcccgaa aatgaataat tcgggatcat
     7921 cttggttctt tggtgtggct tctgaaatca acaataattg ggtatttaat catgatttag
     7981 ttgatgcgat gagaaaagag tcgatgaact ctattctgcc tcacttttct tctacacatt
     8041 ctttagttgc gtcaaaagaa ttccttccta atcaccgcat tcgtgctgaa ttgatactat
     8101 ccaaaaaaga aggccaacat tatgtcacct atcttaacgg gcaattattg aaaaaagacc
     8161 tccatctgaa ttattcgggt gctacattac tctatgatta ccgaaatcct tcgcctaaaa
     8221 atttgtttgg tttcacatta aggacgcatt ggttaggtgg tgcatcactt tgttacctgt
     8281 cggaaagcaa attagctatt ggacaaacca tggttagccc gtctggttac cgaaaaatga
     8341 atatttcttt agtcgcaggt atggaaaagg aagtgttttt tactcgtcaa ttatctcttg
     8401 gggcagggtt aaggttaatg ggcggattaa ctaatatttt caagggatat gagaatattc
     8461 cagcttggtt taatcgcaca cataccggaa atattgctgg aacattatcg gttcgatact
     8521 acctgaatat tcccaatcat taaaaatttc ttctccacac aaccctattt ttagtttcat
     8581 cgtcatttga tttgagagga ctttggtcag gaatctcatc tcaaaccatc tcttttttca
     8641 accaaaaacc ggctgcctta gcaacaagtg acagccggtt ttatttttac caacaagcca
     8701 ttatggtttg tagctttgtg tgatgataac acacagattg ttgcagcgat tggttttagg
     8761 gtttgttttc cttttccctc acatttctat ttctcaagcc tattggtgga aagatgccgt
     8821 attctatgaa gttttcgttc gtagtttcta cgacagcaac ggcgatggaa aaggcgacat
     8881 caacggatta attgaaaaac tcgattacct caacgatggc aatccttcaa cccaaagcga
     8941 tttaggcgta actgctttat ggttaatgcc aatcatgcct tcgcctagtt atcacggata
     9001 cgatgtaaca gattattatg gcgttgaaag cgattatggg aatcgtcagg cattcaaaaa
     9061 cctcatagac agtgcccatg cacgtggaat aaaagtcatt atcgacttgg tgatgaacca
     9121 ttcctccagt tcacatcctt ggtttcaatc tgcattaggc tcggtgaaca gtccgtttcg
     9181 agattattac atttgggaag acagcaatcc tggattcaca ggtccttggg gtcaaaccgt
     9241 ttggcatcct cgaaatggaa aatggaatta tggcattttt tggaacgaga tgcccgattt
     9301 gaattaccga aatacatcag tacaaactga aatgaaatca gtagctcgct tctggacaga
     9361 tagcatgcat gtggatggtt ttcgtttaga tgccattcaa tatttggtag aagatggcac
     9421 acaactatcc ggaacacccg aaacatttgc ctatatcgag gattggagtt caaatttcaa
     9481 aagcgttcat cccgaagtga tgactgttgg cgaaatctgg tcacctactg ctgtaggcgt
     9541 tccttatgtt caagatggtc gcttggatat gtgtttcgaa tttgatcttg cagagtcgat
     9601 tattagcagt ttactagcta acagtccgaa cacctttcaa aacatgatga ataccgtgaa
     9661 cacctcgttt ccggaaagtc ggtatggaac gtttttaacg aatcacgaca ttaaccgctc
     9721 gtttgatcaa ttgggacaaa acactgataa agctaagctg gctgcagctg tttatctcac
     9781 gttgccagga actccattta tctattatgg ggaagaaata ggtatgacag gaaccggtaa
     9841 cgacgaaacc aaacgtaagc ctatgcagtg gagtaatctc gccaatgcag gattttcatg
     9901 gggaacacct tgggagccat tagcaagcaa ttacaatttg gtgaatgtgg cagctcaaca
     9961 acaagacaat acatcccttt ggcatactta ccagcagttt atttctgtca gaaaagagaa
    10021 tacagcctta cgaaaaggtg catacaaggt gatggccgat gtgaatactg aattaattgg
    10081 ctacgcacga tacacaaatg atgagctatt attggtcatt cataatttta ctggaaatga
    10141 cgtatctaat gctgcttttg cactaggagc gaccaatttg ccagaaggaa att
//
LOCUS       DAOOQW010000070        10137 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_8259_length_10137_cov_4.931859, whole genome shotgun sequence.
ACCESSION   DAOOQW010000070 DAOOQW010000000
VERSION     DAOOQW010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 10137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10137
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8259_length_10137_cov_4.931859"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..869
                     /locus_tag="PK281_05730"
     CDS             <1..869
                     /locus_tag="PK281_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48878.1"
                     /translation="DLALKDVRDLYESLLDLRTLAEHQRRKATAEPVTASAPTPVAEP
                     VIEPVAEVKVEIISEPEEEVVQPEKEVAAEIIPVEEKVEEKVVAPEPVAQPKPESIQE
                     EIKAAAEKLPEASPIAWQKTFDGTQVKEEVSKPIVAEPPAAVVTPKVDLLQERQKDFV
                     PTVRKIEFKPESTPNEPKKESIFEKAASLYDKIAKPSEKTVANQATRQPISNIKSSIG
                     INEKFAYLKDLFKNNVNEYNEALDKLNNFENYGEAEDFFQELKAKYNWDPESKSFQGL
                     ADLLNRRYLHNA"
     gene            871..1608
                     /gene="rsmI"
                     /locus_tag="PK281_05735"
     CDS             871..1608
                     /gene="rsmI"
                     /locus_tag="PK281_05735"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015753468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HOY48879.1"
                     /translation="MGKLILVPTPLGNLEDITLRSIRLLKEADVVLAEDTRTSGNLLR
                     LLDISRPLRAFHQHNEHQVLQSTIRLIEESKTVVLISDAGTPGISDPGFLLVRACIEA
                     GIEVECLPGPTALIPALIISGLPADRFCFEGFLPHKKGRQTKIKRLATEERTFILYES
                     PFRLVKSLRQLAEFCGTERKAAVVRELTKMFEECKRGTLTELADYYEKHPPKGEIVLV
                     VGGINQSGDEEEPEEIEESGRAKRKKQ"
     gene            complement(1539..3143)
                     /locus_tag="PK281_05740"
     CDS             complement(1539..3143)
                     /locus_tag="PK281_05740"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOY48880.1"
                     /translation="MLPKQLRFLHPYLHFTGLLLMVAGLPFSLLLMSLSQFFIGGNWI
                     LEGGYKEKWERFKSNKSAWLLCGIFLMLLPGMLWTSNLNDGLKLIRINLPFLIFPFVL
                     ASTKPLKKEWYNLLLKLAVLSVFLATLACTAFGLPRWLNGELADIRTISIFISHIRFA
                     LLIILIALIGMWMLVSNAIPINTYERWGLRIIITWLVVFLFILQSLTGLGILLVLSFL
                     WGMYRLWKKASLLKVLIILFIPITATGIIVWQGLEAWKRYSTPDAMYLLPAPEKSPRG
                     NNYIHDFAVLENGHYVLSYVCDTELYSSWAKRSTQHLDSLDGKGNPTRFTLIRYLNSK
                     GLKKDQDGVNALSDLDIRYIEKGVANVNYTGLWGIRMRFYQLLWELDYSKRGGNKPVG
                     HTIMMKLEFWKNSFSLISENPIYGVGTGDVPDSFKAEYQKSSSWLDPEWWMTSHNHYF
                     YITVALGFPGLICFLICFILPIIRAPRPIYAPLLAFFCIAAFSMLTEDTLTTQAGVSF
                     VAFFYCFFLFARPDSSISSGSSSSPD"
     gene            complement(3193..4215)
                     /gene="hemH"
                     /locus_tag="PK281_05745"
     CDS             complement(3193..4215)
                     /gene="hemH"
                     /locus_tag="PK281_05745"
                     /inference="COORDINATES: protein motif:HMM:NF012964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HOY48881.1"
                     /translation="MKSSKTGVLLIQLGTPDSPSTADVRKYLREFLMDARVIDVPYLV
                     RQVLVNGIIANFRAPKSAKLYQEIWTKEGSPLLIYGKALKHDLQKKLGDTFIVELGMR
                     YQQPPMRKALERFRQAKVNKLILLPLYPQYASSSTGSSLQLAFETIARWEVMPEVHAI
                     SSYFDHQDYIRLWAEKGKALNDGNFDAFMFTFHGVPWRHIRKTECGGTCQDGPKECPA
                     INEHNHVCYRAQCFANARAVRDLAGFPADKTFINFQSRLGKDPWLTPYTDETLIRLAK
                     EGKKRILVFSPSFTADCLETIHEVGTEYNELFKQHGGQELVLVPSMNMDAGWINFLEK
                     MILEKN"
     gene            4373..6631
                     /locus_tag="PK281_05750"
     CDS             4373..6631
                     /locus_tag="PK281_05750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOY48882.1"
                     /translation="MLNSVSASSCFIRNDLVNQALSFGDWEVNLKSGETRWSDYLFSF
                     FEINDHKSFNFFKTNPYYSQEDTLRLTTKANDAIRNHQKTEGVFKVQLPSGKTAWHYS
                     IFQRIEENSADIELLHVTHQDITNQIKLGQKNIGFNPEYNHLIHQLQEIVFQLNAEGC
                     ISFVNDIWEQTTSYSKAETIGKPLKRWVHSHDLDKLDAIFKNLSDKAVSSIEDEIRII
                     DKEGEFIWVSLRLNHSGLETKSLSFFGSMLDINQQKKSTALLLKQQLAIENSREGIAI
                     MNNNDQYTYLNRAHIELFGYSDETEFLGKSWKFIYPPDEIERISNEVFPEFIAKGYYK
                     GTTRGLRKDGTILYQDICLTSLPDGGLMCITRDISEVVKKNEELYRLAIVAEKTNSIV
                     MIGDDKGRVQWVNDSFTEITGFRSEEIVGKRPDEVLNGTETSPETQQEIIDAIQKGTG
                     FQGEILSYKKDGSPIWLLIDVAPIFNSMGRLVNFVAVENDITILKQAEQNTISALEKE
                     RQLNQFKSHFINLASHEFRTPLATIQSSMDILKLRMQKEVQTTSGFRVSFFQHHNRIE
                     QEIKWMTEVMNNILVLGKTDAGRLNVKQEKICMNELIKDVMSDKVSVSEGKDTFSLQI
                     NGECYYMYLDPQLMRHVVYNLLSNALKYTLSDRVPEILIDYLPDELHIHVKDYGIGIP
                     DSEMQHLFTSFFRASNTINIQGIGLGLVIVKQLVEMHGGRVEVTSKYGCGTEFIVILP
                     KEQFENEESINC"
     gene            6609..7265
                     /locus_tag="PK281_05755"
     CDS             6609..7265
                     /locus_tag="PK281_05755"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOY48883.1"
                     /translation="MRKVLIVEDEIVLRNNMEEIISLHGFEVKSAENGEAGLLLARHF
                     QPDLILCDIKMPRYDGFWLIEQIRNEVGLQSVPFIFVSAKVDRRDVREGMELGADDYI
                     TKPFTSNELIQAINSRLDRVKILRSQTDAKESTAAEEIDQKELEKVQEMINRLTPSEK
                     RILKRIAENHSSLEIAQELFISTKTVENHRSNITNKLGLKGHLSLLRFCLNNQKIILG
                     "
     gene            7584..7952
                     /locus_tag="PK281_05760"
     CDS             7584..7952
                     /locus_tag="PK281_05760"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOY48884.1"
                     /translation="MHIFLIDDDGITNMLNQYFFEEHFRDVTISAYTDPEDALDFLMN
                     SKEQPNYIFLDINMPVMDGWEFLENYEKRKIDKEHKPDIYLLSSSVDPADFNRAKTKC
                     YVKGFISKPLEIEKLGFMKK"
     gene            8125..8526
                     /locus_tag="PK281_05765"
     CDS             8125..8526
                     /locus_tag="PK281_05765"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOY48885.1"
                     /translation="MEVGNIWLIDDDPMNNVFNSYLIQDHFPEIQIECFLFAGDALKE
                     LAKFPRYRKFPDLILLDITMPVIDGWDFLQVCTERFPGLRYTTKIYMLSSSENMADRQ
                     KAIEHRFVSGFLSKPLQTSFLLDLIRNHQLV"
     gene            complement(8988..>10137)
                     /locus_tag="PK281_05770"
     CDS             complement(8988..>10137)
                     /locus_tag="PK281_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HOY48886.1"
                     /translation="MALQRLKEAAEKAKVELSSSASTEINLPYVTATATGPKHLVRTL
                     TRAKFEQLADDLIRRTIEPCRTALKNANYSTSDIDEIILVGGSTRIPAIQAAVEKFFG
                     KAPNKGVNPDEVVAIGAAIQGGVLTGEVKDVLLLDVTPLSLGIETMGGVMTRLIESNT
                     TIPTKKSEVFSTASDNQPSVEIHILQGERPMAAQNRTIGRFHLDGIPPAPRGVPQVEV
                     TFDIDANGILHVAAKDKATGKEQKIRIEASSGLSDDDIKKMKAEAEANAEIDRKAKEE
                     AEKINQADTLIFQTEKQLKEYGDKIPADKKGAIETALASLKAAHQSRDLTAIDGALET
                     LNTAWSAASEDMYKATQGADAGASSQPEGQAQNAEVTDVDFEEVKDDK"
BASE COUNT         3137 a         1767 c         2122 g         3111 t
ORIGIN      
        1 tagatttggc tttgaaagac gtaagggatc tttacgaatc actgctcgac ctacgaacat
       61 tggctgagca ccaaagacga aaagctactg ctgagcctgt aactgcatct gctccaacac
      121 ctgttgctga acccgttatc gaacctgttg ctgaagtaaa agttgaaatt atttcagaac
      181 cagaagagga agtggttcag ccagagaaag aagttgcagc tgaaattatt cctgtagaag
      241 agaaagtaga agagaaagtt gttgcaccag aaccggtagc tcagcctaaa ccagaatcga
      301 ttcaagaaga aataaaagct gcagcagaga aattgccaga agcttcacct atagcatggc
      361 aaaaaacatt tgatggcact caggttaaag aggaagtttc aaaaccaatt gttgcagaac
      421 ctcctgctgc tgtagtaact cctaaagttg atttattaca agaacgtcag aaagattttg
      481 ttccaaccgt tcgtaagatt gagttcaaac cggaatcaac accgaatgaa ccgaagaaag
      541 aaagcatttt tgagaaagca gcttcattgt acgacaagat tgccaaacct tctgaaaaga
      601 ctgtagccaa ccaagctact cgtcagccga tttcgaatat taagtcatcg attggtatca
      661 acgagaagtt cgcgtatttg aaagatttgt tcaagaacaa cgtgaacgaa tacaacgagg
      721 cactcgataa gctcaataat tttgagaatt atggtgaagc cgaagatttc tttcaggagc
      781 ttaaagctaa gtacaattgg gatccagaaa gcaaatcatt ccaaggttta gctgatttgt
      841 tgaatcgtcg ctatttgcat aacgcttaat atgggtaaac tcatcctggt gccaacacct
      901 cttggcaatc tggaggatat tacactgcgt tcgattcgtt tgctgaagga agccgatgtg
      961 gttttagcag aagacacacg gacttcggga aatctattac gattacttga tatttcgagg
     1021 cctttacgtg cctttcatca acacaatgaa catcaagtgt tgcaatcgac tattcgtttg
     1081 atagaagaga gcaagacagt tgttcttatt tcagatgctg gaacaccggg tatttctgat
     1141 ccaggatttt tacttgttcg ggcctgtatt gaagccggaa ttgaagtaga atgtcttccc
     1201 ggaccaaccg cattaattcc tgcattaatc atttctggct tacctgctga tcggttttgt
     1261 tttgaaggat ttcttcccca taaaaaaggg cggcaaacta agatcaaacg attagcaacc
     1321 gaagagcgca cgtttatctt gtatgaatca ccatttcgat tggtgaagtc gctccgacaa
     1381 ttagctgaat tttgtggaac tgagcgtaaa gctgcagttg tgcgtgaact cacgaaaatg
     1441 tttgaagaat gcaaacgcgg tactttaaca gagcttgctg actactacga aaagcatcca
     1501 cccaaagggg aaattgtact agttgttggc ggaataaatc agtctggcga tgaagaagag
     1561 ccagaagaaa tagaagaatc gggacgagca aaaaggaaga agcagtagaa aaaagctaca
     1621 aacgaaacac ctgcctgagt agttaacgtg tcttcggtaa gcatcgaaaa cgcagcaata
     1681 caaaagaaag caagtagtgg agcgtagatt ggtcgtgggg cacggatgat gggaagaatg
     1741 aagcagatta gaaagcaaat taatcccgga aatcctaaag ctacagtgat gtagaaatag
     1801 tgattgtgac tcgtcatcca ccattctgga tcgagccagc tggatgattt ttggtattcg
     1861 gctttaaagc tatcgggaac atcgcccgta ccaacaccat aaattggatt ttcgctgatg
     1921 agactaaacg agttcttcca gaattccaac ttcatcatta tcgtgtgacc aacaggttta
     1981 tttccacccc gtttgctata atcaagttcc catagcaatt ggtaaaaacg cattcgtatt
     2041 ccccacaagc ctgtataatt tacgtttgca acaccttttt caatgtagcg aatatcaaga
     2101 tccgacaggg catttactcc atcctgatct tttttaagtc ctttagaatt cagatagcgg
     2161 ataagtgtaa atcgagttgg atttcctttt ccgtcgaggc tatcgaggtg ttgtgtggag
     2221 cgtttagccc aagaagaata caattcagta tcacatacat aactcaacac ataatgtcca
     2281 ttttcgagga cagcgaaatc gtgtatgtag ttgtttccac gaggtgattt ttcgggagca
     2341 ggaagcaagt acatcgcatc gggcgtactg taacgtttcc aagcttctaa accttgccaa
     2401 acgataattc ccgttgcagt aattggaata aatagaatga tgagcacttt aaggagcgaa
     2461 gcttttttcc aaagtcgata cattccccag agaaaggata gaacgagaag aattcccaat
     2521 ccggtaagcg attgtaaaat aaataggaag acaaccagcc acgtgatgat gattcgaagt
     2581 ccccatcttt cgtaagtatt gatgggaatt gcattcgaaa cgagcatcca cataccaatt
     2641 agggcaatga ggataataag aagtgcaaaa cggatatgag agatgaagat ggaaatggtt
     2701 cgtatgtcgg ccagttctcc attaagccat cgcggtaaac caaaagcagt acaagctagt
     2761 gttgcaagaa acaccgaaag gactgcgagt tttaggagca agttgtacca ttcttttttc
     2821 aaaggtttgg tagaagcaag aacgaaagga aagatgagga atggcaggtt gattcgaata
     2881 agtttcaagc catcgtttag attgcttgtc catagcattc ctggcagtag cataaggaaa
     2941 atcccacaaa gcagccaagc acttttattt gatttgaacc gttcccattt ttctttataa
     3001 ccaccttcca gaatccagtt tcctccaata aagaattggg aaaggctcat tagaagtaat
     3061 gaaaatggca agcctgcgac catgagtaac aggccggtaa agtgcaaata ggggtgaaga
     3121 aagcgtagct gtttaggaag cattcgctga tttaaaacag gcggttaaat tggagaaaaa
     3181 tatcggattg cattagttct tttccaagat cattttttcg aggaagttga tccatccagc
     3241 atccatattc atacttggta ctaaaacgag ttcttgtcca ccgtgttgtt tgaagagttc
     3301 attgtattcg gttcccactt cgtgaatggt ttcgaggcaa tcggcagtaa atgaagggga
     3361 aaatactagg atgcgttttt taccttcttt cgctaagcgg ataagcgttt catcggtgta
     3421 gggcgttaac caagggtctt ttcctaaacg cgattgaaag ttgatgaagg ttttatcagc
     3481 cggaaaccca gctagatctc tcactgcccg tgcatttgca aaacactgtg cccgatagca
     3541 aacatggttg tgttcgttaa tagcaggaca ttcttttggt ccatcctgac acgttcctcc
     3601 acattcggtt ttacgaatat gtcgccaagg aactccatgg aaagtaaaca tgaatgcatc
     3661 gaaattaccg tcgttcaagg cttttccttt ttctgcccat aagcggatgt agtcttgatg
     3721 atcgaagtag gaagaaatcg cgtgaacttc cggcattact tcccaacgag caattgtttc
     3781 gaaagctaat tgaagagaag aacccgtgct agatgaggcg tattgcgggt atagcggaag
     3841 taggattagt ttattcactt tggcttgacg aaatcgctct agggcttttc gcatgggcgg
     3901 ttgttggtaa cgcattccta attcaacaat aaacgtatct cccaactttt tctgaaggtc
     3961 gtgttttaaa gccttaccat atatgagaag aggagatcct tccttagtcc aaatttcttg
     4021 gtaaagtttg gcagattttg gagctctaaa attggcaatt ataccattta ccaatacttg
     4081 tcgaactaaa taggggacat caatcacccg agcatccatc agaaattctc taagatattt
     4141 ccgcacatcg gcagttgatg gactgtcggg agttcccaat tgtattaata aaacgccggt
     4201 tttactcgat ttcatagtga atataccttc taaagaaggg aaggcaaatg tagccaatgg
     4261 attgtttgag atgacaagga aatatgggta ttgctgctca tttcagttgt gaaagtgaag
     4321 ttctatgttt gtgtttaagc atttagatgc aaataaatct accaaaccaa ttatgctgaa
     4381 ttcagtatcg gcaagttctt gttttattcg aaatgactta gttaaccaag cattgagttt
     4441 tggtgattgg gaggttaatt tgaagagcgg agaaacacgc tggtcggatt atttgtttag
     4501 cttttttgag attaacgatc acaagtcttt taatttcttt aaaaccaatc catactattc
     4561 tcaagaagat acattacgat tgaccaccaa ggcaaatgat gccattcgaa atcatcaaaa
     4621 aaccgaaggt gtatttaagg tgcaactacc aagtggaaaa acagcttggc attattctat
     4681 tttccagcgc atagaagaaa attcagcgga tatagaattg ttgcatgtta ctcatcaaga
     4741 cataaccaat caaattaagt tagggcaaaa gaatattggg tttaatccgg aatacaacca
     4801 tttgattcat caattacaag aaattgtatt tcaattaaat gcggagggtt gtatctcttt
     4861 tgttaatgat atttgggagc aaacaacctc gtattcaaag gctgaaacga ttgggaagcc
     4921 cttgaaacgc tgggttcatt cgcacgattt agataagtta gatgctattt ttaagaattt
     4981 atcagataaa gccgtttcga gtattgaaga cgaaattcga ataatcgata aagagggtga
     5041 atttatttgg gtttcattac gattgaatca ctcaggttta gagacaaaat cgcttagttt
     5101 ctttggttct atgctggata ttaatcagca aaagaaatca actgcattat tactcaaaca
     5161 gcaactcgcc attgagaatt ctcgagaggg cattgctatc atgaataata acgatcaata
     5221 cacgtattta aatcgtgcac atattgaatt gtttggatat tctgatgaaa ctgaattttt
     5281 aggtaaatcc tggaaattca tttatccacc ggatgaaatt gaacggattt cgaatgaagt
     5341 atttcctgag tttattgcta aaggatatta caaaggaaca actcgaggat taagaaagga
     5401 tggtactatt ttgtatcaag atatttgtct gacatccttg cctgatggag gtttgatgtg
     5461 tataacacga gatataagtg aagttgtaaa gaagaatgaa gaactttatc gtttagcaat
     5521 tgttgcagag aaaaccaatt caattgtcat gattggtgat gataaaggtc gtgtgcaatg
     5581 ggtaaacgat agtttcacag agataaccgg ttttagaagc gaggaaattg ttggtaaacg
     5641 tcctgatgaa gttttaaatg gaacggaaac cagtccggaa acgcaacaag aaattattga
     5701 cgccattcag aaaggaacag gttttcaagg ggagatatta tcttataaga aagatggttc
     5761 gcctatttgg ttgttaatcg atgtagcgcc aattttcaat tcaatgggtc gcttggtgaa
     5821 tttcgtagca gttgagaatg atattacgat actgaaacaa gcagagcaga atacgatatc
     5881 ggctttagag aaagagcgtc aattaaatca atttaaatct cattttatca atttagcatc
     5941 acatgaattc cgtacaccgc tggcaacgat tcagtcgagt atggatattt taaagttaag
     6001 gatgcaaaaa gaggttcaaa caacctcagg attcagagtt tcgttttttc agcatcataa
     6061 ccgaattgaa caagaaatta agtggatgac ggaggtgatg aataacatac tggtattggg
     6121 gaaaacagat gctggcaggt tgaatgtgaa gcaggagaag atctgtatga atgagttgat
     6181 taaagacgta atgagtgata aagtatctgt ttcagaaggg aaggatacgt tttcgttaca
     6241 gattaatggc gaatgttatt acatgtactt ggatcctcag ttaatgaggc atgtcgtgta
     6301 taatttattg tcgaatgcct tgaaatacac attaagtgat cgagttccag agattttgat
     6361 agattattta cctgatgaat tacatattca tgtaaaagac tatggaattg gaatacccga
     6421 ttcggagatg cagcatttat ttacttcatt tttccgagct tcgaatacga tcaatattca
     6481 aggtatcggt ttaggcttgg tcatcgtgaa acagttggta gagatgcacg gaggaagagt
     6541 ggaggttacg agtaaatatg gatgcggaac tgaatttatt gtaattttac ctaaagaaca
     6601 atttgagaat gaggaaagta ttaattgttg aagacgagat cgtgttgcgg aacaacatgg
     6661 aagaaatcat ctcacttcat ggtttcgaag ttaagagtgc agaaaatgga gaggcgggtt
     6721 tattattagc tcgtcatttt caacctgact taattttatg cgatattaaa atgcctcggt
     6781 atgatggttt ttggctgata gaacaaattc gtaatgaagt tgggttgcaa tcggtgccgt
     6841 tcatttttgt ttctgccaaa gtcgatcgtc gtgatgttcg tgaaggaatg gaactaggag
     6901 cagatgatta tatcaccaag ccatttacaa gtaatgagtt aattcaggca attaattcgc
     6961 gtcttgatcg agttaaaata ttacggtcac aaacagatgc caaagaaagc actgcagccg
     7021 aagaaattga tcagaaagaa cttgaaaaag ttcaagaaat gattaatcgt ttaacacctt
     7081 ctgagaaaag aattcttaag cgaattgcag agaatcattc atcattagaa attgctcagg
     7141 aattatttat tagtaccaaa acagttgaga atcatcgttc taatattacc aacaaacttg
     7201 gtttaaaagg gcatttgtct ttattgcgtt tttgcttaaa taatcaaaaa ataattctag
     7261 gataatttta tcttttttga cagaatttaa tttcaaaaat gcgattttat tattcgcagt
     7321 tgatgcgtat tttctattta tagaggtttt cctgatagtt gtttagccta tgaaaaggcc
     7381 agtttttaaa ttgactattt gaataaaatt tattcgaatt aattcattat ttagggcaat
     7441 aaacgaatat tatttaattg ataaatagaa atttaaaggc atttattttt cattagtatt
     7501 ggcacttagg tgttgacatt catttattta cgatataggt attggtagtt aagtagttcc
     7561 tatatttact aaaataaatg gctatgcata ttttcttaat tgacgatgat ggaattacaa
     7621 atatgcttaa tcagtatttt tttgaagagc attttcggga tgtaaccata tctgcgtata
     7681 ctgatcctga agatgcatta gattttttaa tgaattcgaa agagcaaccg aattatattt
     7741 ttttagatat aaatatgccc gtaatggacg gttgggaatt tttagagaat tatgaaaaac
     7801 ggaaaataga taaggaacat aagccagata tttatttgct ctcatcttct gtagatcctg
     7861 ccgattttaa tcgcgctaaa acaaaatgtt atgtaaaggg ctttatatct aagcctcttg
     7921 agattgaaaa attgggattt atgaagaaat gaatttattt ttttgtaata tttccaatgt
     7981 aagttttagg ggcagttgta tgcgcttcgt tttgagtcag ttgcgatcta attaaagcct
     8041 tattaaaatc tgtaaggtaa acgcgtactt tacaaatcct attctatatc ctttgtttag
     8101 ttttcgctcc taatctcaac caagatggaa gtaggaaata tttggctcat cgatgacgac
     8161 ccgatgaata atgtctttaa cagttatttg atacaggatc attttcctga aatacaaatt
     8221 gagtgttttc tttttgcagg cgatgctttg aaagaactcg caaagtttcc acgttatcgg
     8281 aaattccctg atttaatttt attggatata acaatgcccg taatcgacgg ttgggatttt
     8341 ctccaagtct gcaccgaacg cttccctggc ttacgttata cgactaaaat atacatgttg
     8401 tcttcttctg agaatatggc agatcgtcaa aaggctattg aacatcgatt tgtttctgga
     8461 ttcttatcga aacctcttca aaccagtttt ctattggatt taatacgcaa ccaccaattg
     8521 gtttgaattt ttttcaaccc aaattttaat gtaatcggaa atacttggaa agacctgttt
     8581 tattggatta ataagtctac taattgttgc acatttggct tgtttgatcg agctcgtttt
     8641 ccaaaaatga ccggagttga ttttggacat ttaatttgaa atttcatttt ttgaaatagg
     8701 tgtcagccct ctttcattat ctagaattaa tactcacttt tgcaccatta ggttaagtta
     8761 tcacataatt catttactag atacaatcat ttcctgagca gttatgattg atgtaaatga
     8821 gcagttgtca ttctgaatgg gttaggagat caactgattt tatgtgtgct attacccgga
     8881 gtcgcttcag cacaggtggc tccgggtttt tttatgcaat caagttcggg cataaaaaaa
     8941 ccgccactcc caatggaaat ggcggttgat agaataaaaa gcaattatta tttatcgtct
     9001 ttcacttctt caaaatcaac atcagtcact tctgcatttt gagcttgtcc ttctggttga
     9061 gaagaagctc ctgcatcagc tccctgagtt gctttgtaca tatcctcaga agctgccgac
     9121 catgctgtat taagtgtttc taatgcaccg tcaatagctg ttaagtcgcg actctgatgt
     9181 gctgctttta aagaagctaa agcggtttcg attgcacctt ttttatcggc tggaatctta
     9241 tcaccatatt ctttcaattg cttttcagtc tggaaaatca atgtgtcagc ttggttaatt
     9301 ttttcagctt cttctttcgc tttacggtca atttcagcat tggcttcagc ttccgctttc
     9361 attttcttga tatcatcatc acttagtcca gaagaagctt caatacgaat tttttgttct
     9421 ttacctgtag ctttatcttt agctgcaacg tgaagaattc cgtttgcatc aatatcaaaa
     9481 gtaacttcta cttgaggaac accgcgtggt gctggtggaa ttccatctag atggaaacga
     9541 ccaattgtac ggttttgagc agccattgga cgctctcctt gaaggatatg gatttctact
     9601 gatggctgat tatcgctagc ggtagagaat acttccgatt tcttagttgg aatagttgtg
     9661 ttcgactcga tcaaacgtgt catcacacca cccattgttt cgattcctaa tgaaagtgga
     9721 gttacgtcca gtagcaatac atctttcact tcacctgtca atacaccacc ttggattgca
     9781 gctccaattg ccacaacttc atcgggattt actcctttgt taggtgcttt cccgaagaat
     9841 ttctccaccg cagcttggat tgcagggata cgtgttgaac caccaactaa gataatctca
     9901 tcgatatcac ttgtgctgta attagcattt ttcaatgcag tacggcaagg ctctatggta
     9961 cggcgaatta aatcatcagc cagctgttcg aattttgcac gtgttaacgt acgaaccaag
    10021 tgtttaggtc ctgtagctgt tgctgttacg taaggaaggt taatttctgt actagcagaa
    10081 ctagaaagtt ccacttttgc tttttcggct gcttctttta agcgttgaag tgccatt
//
LOCUS       DAOOQW010000071        10078 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_8304_length_10078_cov_4.581263, whole genome shotgun sequence.
ACCESSION   DAOOQW010000071 DAOOQW010000000
VERSION     DAOOQW010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 10078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10078
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8304_length_10078_cov_4.581263"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1953
                     /locus_tag="PK281_05775"
     CDS             <1..1953
                     /locus_tag="PK281_05775"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HOY48887.1"
                     /translation="TEIPLIDFEHLENLPADSARIFSTPVFEGNLISADSKKLTLYLR
                     HEDQLPKSTADSLLLGIESKLKQFGGKTHLMGKLRSEKAYLGKTQQELLLFMSLSFVL
                     VTFFLWLTFRNLWGVLVPLSVVVLSIIWTIGIMAATGKPIDIMIILMPCILFVVGMSD
                     VIHITSQFYEKIEAGFKRIDAIRLALQEVGFATFLTCISTAVAFLTLNTTSIQPIRDF
                     GTYTAIGVVVAYIFSITLLPFILLRVKNPDKFRIHSSQARWDGFLRGTLRAIFKRKAL
                     VIWGSVGVLIFSVAGINRIEINNSVLDDLDEKDPIKEDFRFFDEYFSGVRGFEMSVKP
                     AAGKTSILTWESVQELQKLEFFLENTYQTGALVSPLGLLRGFNQATHDGIAEWYKLPE
                     TRIQFDSLVKTMRPFLGRKEIRLFLSRDEKIGRITGRIKDKGSKAVGKKNTKLERFIQ
                     DASFKQIEVRVTGSSDLIDKSNSYLTENMLQGLSLDIAVLMLIIGLIFRSWRMMLLSI
                     LPNLLPLCMVAGLMGWAGIEMKVTISIIFSIAFGIAVDDTLHLLSRLKIEMAKGQSLP
                     VALKTTYLSTGKAMILTAMVIAAGFSTLMLSDFKSTFYVGMMISVTLIFALIAELVLM
                     PVLLVVLFGKKSTQKKPIQVADLENN"
     gene            2075..2524
                     /locus_tag="PK281_05780"
     CDS             2075..2524
                     /locus_tag="PK281_05780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48888.1"
                     /translation="MKKALFTALLALAFNLGFAAESADLFKVDDQEINTEFAQLNELE
                     QFVNANEGITLEEINPSNPLVQNVNNSSDILGILSTLRGDAPLGIPSFVWGLCLSWVG
                     VLVVYFVADDRDETKKAFTGCAVNAGLYIIWIVVYVVIIGNSFLFFG"
     gene            2683..3231
                     /locus_tag="PK281_05785"
     CDS             2683..3231
                     /locus_tag="PK281_05785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48889.1"
                     /translation="MGSVLYDRSYRKLVYHVTFPSKEDWVIMDTVFNRIVDKKLVARQ
                     FVPMLPSSTIFEFALQNNLDNFGLEKSSYTLSKIDRDGDMVLTTWLPDKRLQKAIGKI
                     IVSKRAGKLVGVAFYTPKNELVKKQIFKGYLKSSGVEFPAEILEILYKVGGKETKIST
                     FKNLRINEMNDDALYNFPVPAK"
     gene            3197..3574
                     /gene="yidD"
                     /locus_tag="PK281_05790"
     CDS             3197..3574
                     /gene="yidD"
                     /locus_tag="PK281_05790"
                     /inference="COORDINATES: protein motif:HMM:NF013932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOY48890.1"
                     /translation="MRYIIFLFLLSNFSIQAQHFGSSELRQGFHKPHVHPDFGFAKNN
                     TNEIQYLFSGLFLFYKFAVSSQDNNKCTFSPSCSEYGMLAVKKKGPILGMLATLDRLQ
                     RCNGLSPKNYTIDQEKNLLIDNP"
     gene            3574..4395
                     /locus_tag="PK281_05795"
     CDS             3574..4395
                     /locus_tag="PK281_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48891.1"
                     /translation="MRNLILFWMLVSFSPAFSQNLYDLANSKEFASYLYRSQQYSLAS
                     IELERLLFIQPDNDTLRETLVRSYMLNKDYVVSIKRLDSFFPNPREIKKPFDDLFAYS
                     LLADKKTERARTFLSSNETLEPEKRQYYQAFSYLLEQDYKQADKLLSEIPNTNTTLAP
                     LRDLSKEGVSMRHKSGGLAMAMSAIVPGSGKFYTGDWKDGIISLISIGVTGYQAIHGF
                     QVKGTKSAYGWIYGTIATGFYLGNIYGSFTSAKRFNKRQSDRVAQKVDAAFSIQP"
     gene            4370..5197
                     /locus_tag="PK281_05800"
     CDS             4370..5197
                     /locus_tag="PK281_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48892.1"
                     /translation="MLLLVFSLSILFPRASAQDLEETYRFGLEQYAISNYESATLAFE
                     RILYFGKGQYIISTLDHLARIKLAQGDPESAIKYYHRASVTADEYAEKSWYTLRKCAG
                     LLSLQKTKLAMVDLYDITDNLPDSTLKYKYFLLGVAHFAELNFAESRIAFKQALPETE
                     VNKRNSLDSLFNVVQDIKHPKAKTAKILSIIIPGAGQFYAGDIKNGVNSLLLTGGFMF
                     LGINTAVKYGFMNSFVSVFPWFQRYYMGGYHRAERIAVERLAEKRDYIYQEVLNLYQ"
     gene            complement(5205..5927)
                     /locus_tag="PK281_05805"
     CDS             complement(5205..5927)
                     /locus_tag="PK281_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013187351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HOY48893.1"
                     /translation="MDKELNKKPVLVVLTGAGISAESGIRTFRDSGGLWEEHSIMDVA
                     TPEGWRKNPALVLDFYNQRRKQLEEVVPNAAHVALVKLENYFDVHVITQNVDDLHERA
                     GSTQILHLHGELIKARSERYEDNIYTIGYAPIQLGQTCEHGTQLRPHIVWFGEDVPMI
                     NTATELVKQAEILLVIGTSLNVYPAAGLLYHAPYDSRKYLVDPSENTTGQIYRLTHIQ
                     DLAGKAVPKLVEQLIAEYQSTQ"
     gene            6041..8347
                     /locus_tag="PK281_05810"
     CDS             6041..8347
                     /locus_tag="PK281_05810"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HOY48894.1"
                     /translation="MYYLIQIAGYQKNMDDYKKKQALDYHANGRPGKIEVISSKPTST
                     QRDLSLAYSPGVAEPCLEIEKNPDDVYKYTTKGNLVAVISNGTAVLGLGNIGPEASKP
                     VMEGKGVLFKIFADIDVFDIEVDATDIDKFVDTVKAISPTFGGINLEDIKAPECFEIE
                     RRLKESLKIPVMHDDQHGTAIITGSALLNALEIAGKKIAEVKVVFNGAGASAISCARL
                     YLQLGVKKENLIMCDSTGVISTSRTDLDSQKQFFAANTDKKTLTEAFVNADVFVGLSK
                     GNIVNQDMIRSMAKDPIVFALANPDPEIPYHDAIAAREDIIMATGRSDYPNQVNNVLG
                     FPFIFRGALDVRATSINEEMKLAAVYAIANLAKQPVPEQVNMAYNKANLQFGREYIIP
                     KPVDPRLITYVAPAVAQAAMDSGVAKNPIADMEAYKLELIRRMGLDNSLMDRLTTRAK
                     TNPKRIIFAEADHHKILKAAQVVNNEGIAIPILLGNRIKIKEICDEFNINLDGVEIID
                     PRDSEQDDRRHAFGDIFYDLRKRRGITLYEARKMMRERNYFGAMMVRTGAADALISGL
                     TRRYPDTVKPALQVIGIRENVSRVAGLYIIMTKNGPVFFSDTTVNINPTAEELVDITI
                     LTADAVSNLNITPKIALLSYANFGSAEGADALKMREVARMLHERRPDLIVDGELQANF
                     AMNNNMLKEQFPFSCLVDNKVNTLIFPNLAAGNIAYKLMQELASAEAIGPLLLGMKKP
                     VHILQLGSSVREIVNMVTLAVVDAQAHS"
     gene            8351..8935
                     /gene="ruvA"
                     /locus_tag="PK281_05815"
     CDS             8351..8935
                     /gene="ruvA"
                     /locus_tag="PK281_05815"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010518470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HOY48895.1"
                     /translation="MYEYFSGKLIEKTPTYAVIDCGGVGYVLNISLSTFSQITNPDKC
                     LLYAHLAVREDAHVLYGFATRSERETFRLLINVSGVGAGTARMILSSLSSSEIAEAVM
                     SNNVTTLKNVKGIGEKTAQRIIVDLKGKFDRDSVSPELFAGLHNTVRSEALTALTLLG
                     FPKINAEKALDKVIKSAGQELSVEDMIKQTLKML"
     gene            8977..>10078
                     /gene="sprA"
                     /locus_tag="PK281_05820"
     CDS             8977..>10078
                     /gene="sprA"
                     /locus_tag="PK281_05820"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HOY48896.1"
                     /translation="MKTYYKYLSGSIAALLFMAVAWSANSRPFGWVEEEFRFQPESLV
                     GDTTDPDSLLPFPFSDDELDPLDPSPNGLFLADPSNIETTIELNPKTNEYDVNQKMGD
                     MRYGYPSYLTFSEYQEMDFDAALQKNWRDRMASDDASKPKKGFQPKIIVPGDAFATIF
                     GSNTIDIRPQGSAELIFGGNFNRTQNPALPIRQQRVGAFNFQQKIQMNIVGKIGDKIQ
                     LQTNYNTETGFNFDNKVNLKYEGKEDEIIQLLEAGNVSMPLTGSLITGSQSLMGVKTK
                     LKFGRLTATTIFSQQQGDKKTINVQGGAQTTQFDISASDYDANRHYFLNQFFKDRYDQ
                     SMSTLPNVTSPFNVTRVEVYITNRTYATTNVRN"
BASE COUNT         3104 a         1826 c         2039 g         3109 t
ORIGIN      
        1 acggagattc cattaatcga ctttgagcat ttagaaaatc ttccggctga ttcggctcgg
       61 atatttagca caccagtttt tgaagggaat ttgatttcgg cagatagtaa gaagctaacc
      121 ttgtatttgc gtcatgaaga tcagctaccc aaaagcactg cagatagttt attgctaggc
      181 atcgaaagca aactaaaaca gttcggaggc aaaacccatt taatgggaaa actccgttcc
      241 gaaaaagcct atctaggaaa aactcagcag gagcttttgc tgttcatgtc gttatcgttt
      301 gtattagtca cttttttcct ttggctaaca ttccgaaatc tatggggtgt tttagttccc
      361 ctatcggtag tggttttatc catcatctgg acgattggaa tcatggctgc tacaggcaaa
      421 ccgattgata ttatgatcat tctcatgcct tgtattttgt ttgtggtagg catgtcggat
      481 gtgattcata ttacaagtca gttttacgaa aaaatagaag ccggatttaa gcggatagat
      541 gcgataagat tggcacttca ggaagtcgga tttgctacgt ttctaacctg tataagtacg
      601 gctgttgcat ttttaacttt aaataccaca tctattcaac cgatacgcga tttcggaacc
      661 tatacagcaa taggagttgt ggttgcgtac attttctcca tcacattatt accattcata
      721 ttacttcggg ttaagaatcc ggataaattt agaattcact cttcacaagc ccgttgggat
      781 ggatttttac ggggcacttt acgagcaatt ttcaagcgaa aagctcttgt gatatggggt
      841 tcggttggag tactgatttt ctctgtagca ggaattaatc gtatcgagat taacaattcg
      901 gtgttagatg atttagatga aaaagacccg ataaaagaag attttcggtt ttttgatgag
      961 tatttctctg gagttcgggg gtttgaaatg tcggtcaaac ccgcagctgg aaaaacgagt
     1021 attctaacat gggagtcggt tcaagaactg cagaagttgg agtttttcct tgaaaacaca
     1081 tatcaaacag gagcattagt ttcccctttg ggtttgctaa gaggattcaa tcaagcaacc
     1141 cacgatggta tagctgaatg gtataaacta cctgaaactc gcatacaatt cgactcattg
     1201 gtaaaaacca tgagaccttt tttaggaaga aaggaaattc gtttgttttt atcgcgggat
     1261 gaaaaaattg gtcggattac aggccggata aaggataaag gcagcaaagc ggtaggaaag
     1321 aagaatacaa agcttgagcg atttatacaa gatgcgtctt tcaaacagat agaagttcgt
     1381 gttacgggtt cttccgattt gattgataaa agcaatagtt atttaactga aaacatgctt
     1441 caaggcctca gtttagacat agcggttttg atgttgatta ttggattgat tttccgttcc
     1501 tggcggatga tgttattatc gatattgcct aatttactgc ctttgtgtat ggtagcagga
     1561 ttaatgggct gggctggaat tgagatgaag gtaacaattt cgataatctt ctccattgca
     1621 tttggtatag cagtagatga tacacttcac ctactgagca ggttaaaaat cgaaatggca
     1681 aaaggacaga gtttgccagt tgcattaaag actacttatt tatctactgg aaaggcaatg
     1741 atcttaacag caatggtgat agcagcggga ttttcaacat tgatgttgag tgattttaag
     1801 agtacttttt atgtgggaat gatgatttcg gtgacattaa tttttgcatt aatagccgaa
     1861 ttggtgttaa tgccagtatt gttagttgtt ttgtttggaa aaaaatctac acaaaaaaag
     1921 ccaatacaag ttgctgattt agagaacaac taatcaaatt tagatgaaaa tgtttgatgt
     1981 tagagggtga aattttcata cagaaaatcc atataaatgt tgaagtaatt acaaaccgtt
     2041 tatatttgcg gataagaaca aaaagcaaaa ctttatgaaa aaagcactat ttactgcgct
     2101 tcttgcgctt gcattcaacc tcggctttgc tgcggagagt gccgatctat tcaaggtaga
     2161 tgatcaagaa atcaacactg aatttgctca attaaatgag cttgagcagt ttgtaaatgc
     2221 taacgaaggc attactcttg aggaaatcaa ccctagtaac ccattagttc agaatgtaaa
     2281 caactcaagt gacattctgg gtattttatc aactcttcgt ggagatgctc cactagggat
     2341 tccttctttc gtttggggtc tttgcctttc ttgggtagga gtattggtag tttactttgt
     2401 agctgatgat cgtgatgaaa ctaagaaagc atttacaggt tgtgctgtga acgctggtct
     2461 ttacatcatc tggattgttg tttacgtagt aattatcgga aattcattcc tattcttcgg
     2521 atagaattaa tgaatcactc aagtcaaaac aagttttgtt ttgtcttcac cttactagta
     2581 attctaatgt tgcccaattt ttcaattggg caacattatt ttaggttaag ggcagacttc
     2641 acaattaagg agaaattcag cgatgggaaa atgagcctta caatgggttc agttctttac
     2701 gatcgctctt atagaaaatt agtgtaccat gtcacttttc cttcaaagga agattgggtg
     2761 attatggata ctgttttcaa tcgaatagta gataaaaagc tggtagctcg acagtttgtt
     2821 ccgatgttac catcttcaac gatatttgag ttcgcattac aaaacaatct ggataatttc
     2881 ggattggaaa aatcttctta cacgttgagt aaaatcgatc gtgatggaga tatggtttta
     2941 accacttggt tgcctgataa acgacttcaa aaagctatcg gtaaaattat tgtttcaaaa
     3001 cgagctggta aattagttgg agtagcattc tacacaccta aaaacgagtt agttaaaaaa
     3061 caaattttta aggggtattt gaaaagctca ggagttgagt tccctgctga gatactcgag
     3121 atactttata aagttggtgg aaaagaaacg aagatttcaa cttttaagaa tcttcgcatt
     3181 aatgaaatga atgatgatgc gttatataat tttcctgttc ctgctaagta attttagcat
     3241 tcaggctcaa catttcggct catcagaatt gcgacaaggt tttcacaaac ctcatgttca
     3301 tcccgatttt ggttttgcta aaaacaacac caacgaaatc caatacctgt tttcgggttt
     3361 attccttttt tacaagtttg cagtatcttc tcaagataac aacaagtgta ctttcagccc
     3421 atcttgctca gaatacggta tgttagctgt taagaagaaa ggccctattt taggaatgct
     3481 tgctacactc gatcgtttac aacgttgtaa cgggttgagt cctaagaatt acaccatcga
     3541 tcaggagaaa aacctgctta ttgataatcc ctgatgcgga atcttattct tttttggatg
     3601 ctggttagtt ttagtccggc attttctcag aatctctacg acttagctaa ttcaaaagag
     3661 tttgcaagct atctctatcg ttctcaacaa tattcattgg cttcgattga attagaacga
     3721 cttctattta tccaacccga taacgataca cttcgagaaa cactcgtgag aagctacatg
     3781 ttaaacaaag actatgtagt ttccattaaa aggcttgatt catttttccc aaacccaaga
     3841 gaaattaaaa agccatttga tgaccttttt gcttactcct tgttagcaga taagaaaact
     3901 gaacgtgctc gtacattttt gagttcaaac gagacattag agcctgaaaa gcgtcaatat
     3961 tatcaagctt ttagttattt gcttgaacaa gattataagc aagctgataa actactttcc
     4021 gaaattccca atacgaatac gactttagct cctttacgag atttaagtaa agaaggagtt
     4081 tcgatgcgac ataaatcggg tggtttagcc atggcgatgt cggcaattgt tccaggaagt
     4141 ggtaaatttt atacgggtga ttggaaagat ggaattataa gtctgatttc tattggagtt
     4201 acaggttatc aagctattca tgggtttcaa gtgaaaggta ccaaaagcgc ttatgggtgg
     4261 atttatggca ctattgcaac aggattttac ttagggaata tttatggttc atttacctca
     4321 gctaagcgtt tcaacaaaag acaatccgat cgggttgctc aaaaagttga tgctgctttt
     4381 agtattcagc cttagtatac tattccccag agcttctgct caagatctag aagaaacata
     4441 tcgttttggt ctggaacaat atgctatttc aaattacgaa agcgccacgc ttgcatttga
     4501 acgaattctg tattttggaa aaggacaata tattattagt acgcttgatc atttagctcg
     4561 aataaaactt gcccaaggcg atcccgaatc tgccattaaa tactatcatc gagcatctgt
     4621 tacagccgat gagtacgctg aaaaatcttg gtatacacta agaaaatgtg ctggtttatt
     4681 gtcattacag aaaaccaaat tagccatggt tgatttgtat gacattactg ataacctacc
     4741 tgatagtact ttgaaataca agtacttctt attaggagtt gcacactttg cagaattgaa
     4801 tttcgctgaa agccgaattg cgtttaaaca agctttgcct gaaacagaag taaataaacg
     4861 aaattcctta gatagtttgt ttaatgtagt tcaagacatc aaacacccta aagccaaaac
     4921 agctaaaatt cttagtataa ttattcctgg agctggacaa ttttatgctg gtgatattaa
     4981 aaatggagtt aattcacttc tattaacggg tggtttcatg tttctgggca ttaatacagc
     5041 cgttaaatat ggatttatga attcgtttgt ttccgtattc ccttggtttc aacgctatta
     5101 catgggcgga taccaccgcg ctgaacgtat tgcagttgaa cgattagctg aaaaacgtga
     5161 ttatatttat caagaagtat tgaatctata tcaataactt attcctattg agtagattga
     5221 tattcagcaa ttaattgctc tactaattta ggaaccgctt tccctgctaa atcctgaatg
     5281 tgcgttaaac gataaatctg tccagtcgta ttttcagagg gatctactag gtactttcta
     5341 ctatcataag gagcatgata taataatcca gcagcaggat aaacattcaa agatgtccca
     5401 attaccaata agatttctgc ttgtttaact aattctgtag cagtatttat catcggaaca
     5461 tcttctccaa accatacaat atgcggacga agttgggtgc cgtgttcaca tgtttgacct
     5521 agttgaatag gtgcgtatcc gatcgtataa atattatcct catacctttc acttctagct
     5581 ttaataagct ctccatgtag gtgtaaaatt tgagttgatc cggcacgttc atgtaaatca
     5641 tctacatttt gagtaatgac atgtacatcg aaatagtttt ccaatttgac taatgcaaca
     5701 tgagccgcat ttggaaccac ttcctccaat tgttttcttc gttgattata aaagtcgagt
     5761 accaaggcag gatttttacg ccaaccttcg ggtgttgcca catccataat ggaatgctct
     5821 tcccataatc caccactatc tctaaatgtc ctaatgccac tttcggcact aattccggct
     5881 ccagtcagta ctactaaaac aggcttttta ttaagctctt tatccattct taagtaatct
     5941 ttaaaaatag aattaaaata caagtactgc aatgttgaaa accatcagta ataaactgcc
     6001 taaaatggac tgatttcttt tacttttgcg gcttgcagct atgtattatc taatacaaat
     6061 tgctggatat caaaagaata tggacgatta caaaaagaaa caagcgctcg attaccatgc
     6121 aaatggtcgt ccgggtaaaa ttgaagtcat ttcaagtaaa cccacatcaa ctcaacgaga
     6181 tttatctcta gcctattcac caggtgtcgc tgaaccttgt cttgagatag aaaaaaatcc
     6241 cgatgatgtg tataaatata caaccaaggg aaatctagtt gcggtaatat caaatggtac
     6301 agccgtttta ggattaggaa atatcgggcc ggaagcatct aaaccggtga tggaaggaaa
     6361 aggagtccta tttaaaattt tcgccgacat tgatgtattt gatattgaag tcgatgcgac
     6421 tgatattgat aagtttgtcg atacggtaaa agctatttca cccacgtttg gaggaattaa
     6481 tctggaggat ataaaagctc cggaatgttt tgagattgag cgtcgtttaa aagaatcatt
     6541 gaaaattcct gtcatgcatg atgatcagca tggaacagct attatcactg gttctgcctt
     6601 gttgaatgca ttagagattg caggcaaaaa aatagctgaa gtaaaagttg tgtttaatgg
     6661 agcaggtgct tcggcaatat cttgtgcccg tttgtatttg cagttaggag ttaaaaagga
     6721 aaatctaatt atgtgcgaca gcactggtgt gataagtact tcccgaacag atttagattc
     6781 tcaaaaacag ttctttgctg ccaatacaga taaaaagaca ttaacggaag cattcgttaa
     6841 tgcggatgtt tttgtaggat tatcgaaagg aaatattgtg aatcaagata tgattcgcag
     6901 tatggcgaaa gaccctattg ttttcgcgtt agctaatcct gatcctgaga ttccatatca
     6961 cgatgctatt gcagcgcgtg aagatattat catggcaact gggcgttcgg attatcctaa
     7021 tcaggttaac aatgtattag gtttcccgtt tattttccga ggagccttgg atgttcgagc
     7081 tacgagtatc aacgaagaga tgaagttagc agccgtttat gcgattgcta atctggctaa
     7141 acaacctgtc ccagaacagg tgaatatggc ctataacaaa gccaatcttc aatttggacg
     7201 tgagtatatt attccaaaac cagttgatcc aaggcttatc acttatgtag ctccagcagt
     7261 tgctcaagca gctatggatt cgggtgttgc gaagaatcct attgctgata tggaggctta
     7321 taaactcgag ctaatacgtc gaatggggct tgacaactcc ttaatggatc gtttaacaac
     7381 tcgggctaaa acaaatccga aacgtatcat attcgcagaa gcagatcatc ataaaatact
     7441 aaaagcagca caagtagtta acaacgaagg aatagctatt ccaatccttc ttggaaatcg
     7501 gattaagatt aaagagatat gtgatgaatt taacatcaat ctagatggtg ttgaaataat
     7561 tgacccgcgc gattctgaac aagatgatcg ccgtcatgca tttggagata tcttttatga
     7621 cttgcgcaaa agacgcggta ttaccttgta tgaagcaaga aaaatgatgc gtgaacggaa
     7681 ttactttgga gccatgatgg ttcgaacagg tgccgctgat gcattgattt caggtttaac
     7741 tcgtcgctac cctgatactg ttaaacctgc tttacaagtt attggcattc gcgaaaatgt
     7801 gagtcgtgtg gctggtttgt acatcatcat gacgaaaaat ggtcctgtat ttttctccga
     7861 cacaaccgta aacataaatc ctacagcaga agaattggtt gatatcacca tcctcactgc
     7921 tgatgcggta agtaatctta acatcactcc taaaattgca ttgctatcgt atgcgaattt
     7981 tggttctgcc gaaggtgctg atgcactaaa aatgcgcgaa gtagcacgca tgctccatga
     8041 gcgtcgtcca gatttaattg tagacggtga gttgcaagca aattttgcga tgaataataa
     8101 tatgctgaaa gaacagtttc ctttttcgtg cttagtggat aataaggtaa acaccttgat
     8161 tttccctaat ctggcagctg ggaatattgc ttataaactc atgcaagaat tagcatcagc
     8221 cgaagcaatc ggtccattgt tgttgggcat gaaaaaacca gttcacattt tacaattagg
     8281 tagctctgtt cgtgaaattg tgaatatggt taccctagca gttgttgatg cacaagctca
     8341 ttcttaattt atgtacgaat attttagtgg taaattgatt gaaaaaacac ctacctacgc
     8401 ggttatcgac tgtggaggag ttggttatgt actgaatatt tcactgagta ctttttcaca
     8461 aattaccaat ccagataagt gccttttgta tgcccattta gctgttcgag aagatgctca
     8521 cgtactatat gggtttgcca cacgctccga acgagaaaca tttagactgt taataaatgt
     8581 atccggagtg ggtgccggta ccgcgcgtat gattttatct tcgctaagtt cctccgaaat
     8641 tgctgaagcc gtaatgagta ataacgtcac cactttgaaa aatgtaaaag gtattggtga
     8701 aaaaactgct caacggatta tcgtcgattt aaagggcaaa tttgaccgcg attcggttag
     8761 tccagaactt tttgcaggct tacacaatac agtaagaagc gaagcgttaa ctgcactgac
     8821 tttactcggg tttcctaaga ttaacgcaga aaaagctcta gataaagtaa ttaaatcagc
     8881 cgggcaggaa ttgagcgtag aagacatgat caagcaaacc ttgaaaatgc tttaacgtat
     8941 caagtcccgt ttgtctgtta ttgaaccatt gattgattga aaacgtacta taaatatctt
     9001 tcaggaagta ttgctgcatt gctcttcatg gctgttgctt ggagcgcaaa cagtcgccct
     9061 ttcggttggg tcgaagagga atttcgtttt caaccagaat cactagttgg tgatacaacc
     9121 gatcccgata gtttacttcc attccctttt tcggatgatg aattggatcc tttagatcca
     9181 tcacccaacg gtttgtttct agccgaccct tccaacattg aaacgaccat cgaactcaat
     9241 cctaaaacca atgagtacga tgtaaatcag aaaatgggtg atatgcgtta tggctatccg
     9301 agctatctca ccttctccga atatcaggaa atggatttcg atgcagcact tcagaaaaac
     9361 tggcgtgatc gcatggcttc tgatgatgcg agtaaaccta agaagggatt tcagcctaaa
     9421 atcatcgttc cgggtgatgc atttgctacg attttcggaa gcaataccat cgatattcgt
     9481 cctcaaggat cagctgaact aattttcgga gggaatttca accgaaccca aaatccagcc
     9541 ttacctattc gtcaacaacg tgttggagca ttcaatttcc aacagaagat tcagatgaat
     9601 attgttggga aaataggcga caaaattcag ttacaaacga actataacac ggagactggc
     9661 ttcaatttcg acaataaagt caatttgaaa tatgaaggta aagaggatga aatcatccaa
     9721 ttactggaag cgggtaatgt gagcatgcct ttaacaggtt cacttatcac gggtagtcag
     9781 agtttgatgg gtgttaaaac caagctgaaa tttggtcgtt taacggctac cactattttc
     9841 tcccaacaac agggcgataa aaagactatc aatgttcaag gtggagctca aaccacacaa
     9901 tttgatatct cagcatccga ttacgatgct aaccgacatt atttcttaaa tcagtttttc
     9961 aaagaccggt acgaccagtc gatgagtact ttaccaaacg ttacatctcc tttcaacgta
    10021 acacgtgtcg aagtttatat caccaaccgc acatacgcta ctaccaatgt tcgtaaca
//
LOCUS       DAOOQW010000072         9719 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_8694_length_9719_cov_4.927256, whole genome shotgun sequence.
ACCESSION   DAOOQW010000072 DAOOQW010000000
VERSION     DAOOQW010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 9719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9719
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8694_length_9719_cov_4.927256"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(22..1341)
                     /locus_tag="PK281_05825"
     CDS             complement(22..1341)
                     /locus_tag="PK281_05825"
                     /inference="COORDINATES: protein motif:HMM:NF022466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HOY48897.1"
                     /translation="MISEQLLHFIWKFRLYQTDKLALASGESLEVISPGTHNKHSGPD
                     FQNARIKIGNTLWAGNVELHIRSSDWYRHKHETDAAYHTVILHVVAIHDMDVFDLNNQ
                     VIPVIELKSFIKESLLQRWEELSGSMFPIPCAALGRPDVITMQNWLDRLVTERLEEKS
                     SRIRQLLSLTKNDWTEALYCFIFRSVGMQLNAVPFELLARSTPYRILLNYKQSQFQLE
                     ALLYGQSGLLHSEHEDEYLQALWNEYRFLARKHKLKPIEKHLWKFLRLRPMNFPTVRI
                     SQLANLISKHSISPENFREMGSLKEWVNWFDMECSPYWDEHYVMDHFSTKKKKHFGKS
                     ASQSLLMNAIVPFLFVYATHRGDEELRDRVLEVLYQLPPEKNVILEVWNQFGIQPDTA
                     AASQGLIQLKTNYCDSRRCLECAVGHRLLKEETRREPLSSNGKVPTF"
     gene            complement(1692..2504)
                     /gene="pyrF"
                     /locus_tag="PK281_05830"
     CDS             complement(1692..2504)
                     /gene="pyrF"
                     /locus_tag="PK281_05830"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019388086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HOY48898.1"
                     /translation="MTRSELISFIWKQKSYLCVGLDTDITKIPSHLLNEADPVFEFNK
                     AIIDATKEYCVAYKPNIAFYESMGAAGWESLRKTLEYIPSTHFTIADAKRGDIGNTSA
                     MYARAFFDTYSFDSVTVAPYMGKDSVQPFLDRKDKWTILLALTSNSGAEDFEQLKTTS
                     GKYVFEEVLSKASEWGSSEQLMFVAGATRVEELQHVRSLVPDHFLLIPGVGAQGGNLK
                     DVSKASLTKDVGILVNASRSILYASSGIDFREAAAVEAQSMQLEMKSLLEGI"
     gene            complement(2501..>3166)
                     /locus_tag="PK281_05835"
     CDS             complement(2501..>3166)
                     /locus_tag="PK281_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HOY48899.1"
                     /translation="CENKGWKVDVIDQSEGTLGGFKEIVMEVSGDNVFGVLKYESGVH
                     RVQRVPATETQGRVHTSAATVVVLPEADEVDVVINPADLEFQTSRSGGAGGQNVNKVE
                     TKVQLTHKPTGIMVMCQIERSQLGNKIRAMNMLRAKLYDLELTKHNNAISKRRKTMVS
                     TGDRSAKIRTYNYPQSRVTDHRIGFTMYNLPAFVGGDIEELINALQVAENAERMQEGT
                     ESL"
     assembly_gap    3167..3266
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3267..3651)
                     /locus_tag="PK281_05840"
     CDS             complement(<3267..3651)
                     /locus_tag="PK281_05840"
                     /inference="COORDINATES: protein motif:HMM:NF015427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HOY48900.1"
                     /translation="MTRFKRLNKEYKDLQPVVESGIRYKKILEDLKSANELMYAEADE
                     AFRAMAKDEINLLEAEQIALEETIRLLLIPKDPEDGKNAVLEIRAGTGGDEASIFAGD
                     LARMYIKFCENKGWKVDVIDQSEGTL"
     gene            complement(3768..4943)
                     /locus_tag="PK281_05845"
     CDS             complement(3768..4943)
                     /locus_tag="PK281_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HOY48901.1"
                     /translation="MQTHDRYAARGVSSGKEEVHAAIRNSDKGLFPKAFCKIIPDTLT
                     GDPEYALVMHADGAGTKSSLAYIYWKETGDLSVWKGIAQDALVMNTDDLLCVGATDQI
                     LVSSTIGRNKSNIPGEVISAIIQANQELCDWFSSFGIHVHLTGGETADVGDIVRTLIV
                     DSTVVARIKRSDIIDNSRIQAGDVIVGLSSSGQATYEDRYNGGMGSNGLTSARHDVFS
                     SVYRSKYPESYNPEVPEELVYSGKYLLTDQVPGLSVNYGQLVLSPTRTYAPIIQQILE
                     KHRPQIHGMVHCSGGGQTKVLHFIDQLHIIKDSLFDVPPLFQIIQESTGTDWKEMYRV
                     FNMGHRMELYVPSEIAESIIEISHSFGVEAKIIGRVESSSKKALTIQTVQGNFHYEA"
     gene            complement(5009..7141)
                     /locus_tag="PK281_05850"
     CDS             complement(5009..7141)
                     /locus_tag="PK281_05850"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOY48902.1"
                     /translation="MLALSASTLFAQPKRVKEADRLFNNKEFYEALDAYKKASENIRG
                     NKALKAEVTYKQALCYMAFKDTKKAEVWFAKAIKAKYQDPIAILFLADMMRENAKYDE
                     ALAEYDKYVKLKPEDPRGTSGVESCKLSVKWKAAPTKHEVSNVQPLNSKFDDFSPIYS
                     RKNFKEIIFTSSREGSTGSGTDGWTGEAFSDLFEAKLDKNGKWSTPTSFKEPLNSKYN
                     DGASSLNDKFSVMFFTRCSYDKNKIKGCQIFTTRKKGNNWDEPILVPFAADSFTVGHP
                     WISDDEQTLYFASDMPGGLGGRDIWMAKFDKKSKAWGSPVNLGPQVNTIGDELYPTLR
                     NDSTLYFSSNGLVGMGGLDLFEAKFVKGKWSSVSNLKFPLNSEADDFGIIFQGKQERG
                     YFSSSRLGGRGGADIYEFSVPPILFTLGGYVYDFDNKTPVTGAKVTMVDKDGMTYDAI
                     TDQTGSYYFDNTKFREDNTYELLASATDFLNDKGTETTVGETAGRDYKKDFFLKTTKK
                     GPIKLPKIEYDLGKWDLRPESKDSLNGLVQTLKDNSNITIELMSHTDSRDAAKANIVL
                     SQKRAQSVVDYLIEQKIDPSRLTAKGYGETKLLNRCKDGVKCSEEEHQVNRRTEFRIT
                     STTFVPAVGSPEFKAPKIESVDEDTEIETDVEEIKRDNLQVDPKSAPVPPAAPAEPAP
                     AKPTPAAAPAPAPAKPAPAPAVPAKPKK"
     gene            complement(7340..9535)
                     /locus_tag="PK281_05855"
     CDS             complement(7340..9535)
                     /locus_tag="PK281_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007649732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HOY48903.1"
                     /translation="MSTIRFKALESLINRTYEPNGPSAEKISDYYGVHVFGDISMREF
                     LSKDAYDKVRGAIEQGTQIERHVADQVASSMKAWAMNMGCTHFTHWFQPLNGATAEKH
                     DSFFEPLADGRAIENFSGSNLIQQEPDASSFPSGGIRNTFEARGYTAWDPTSPAFIIS
                     GTLCIPTIFVSYTGEALDFKTPLIKALHALDKAAVDVCQYFDKNVTKVTATLGWEQEY
                     FLIDEALFHARPDLSLTGRTLFGHSPAKGQQLEDHYFGTIPDRVAAYMRDFETECHKL
                     GIPVRTRHNEVAPNQFECAPIFEEANLAVDHNQLLMDVMSKVARRHRFRILLHEKPFA
                     GINGSGKHNNWSLSTNTGKNLLSPGKTPKNNLQFLTFFITTIKALHRYADLMRAAIAS
                     AGNDHRLGANEAPPAIISAFIGSQLTGVLDDLEQRVKASGKMTPDEKTDLKLNIGKIP
                     QILLDNTDRNRTSPFAFTGNKFEFRAVGSSANCSQSMIVLNTIVTQQLRDFKIEVDGL
                     IAKKKLDKDEAIFQVLRRYITESKAIRFEGNGYSDEWVKEAAKRGLNNFAKTPEALDA
                     FVSKPSLKLFEENDVLSHREVEARYEIQLEMYVKKIQIESRVMGDLAANHIVPTALKY
                     QSALIQNVKGLKDIMDAKTFQKSAQAQLEIITEISEHVNAIKKMVDEMIESRKQANKI
                     ADVRKLATAYCTKVQGFFDPIRYHVDKLELLVDDEIWCLPKYREMLFIN"
BASE COUNT         2847 a         2043 c         1759 g         2970 t
ORIGIN      
        1 tccaagttag cattcctgaa ttcaaaatgt cggtacctta ccgtttgaac taagaggttc
       61 ccttcgggtt tcttctttaa gcaaacgatg tcctaccgcg cattctagac atctgcgcga
      121 atcacagtag tttgttttta gttggatcaa gccttgagat gctgcagcag tatcgggctg
      181 aatcccaaat tgattccaca cttccagaat aacatttttc tctggtggaa gttgatacaa
      241 aacttctaaa acacgatcac gaagctcttc atcacctcga tgagtagcat aaacaaacag
      301 aaaaggcact attgcattca taagcaaact ctgacttgca gattttccga aatgcttttt
      361 cttctttgta gaaaagtggt ccattacata atgttcgtcc caataaggag aacattccat
      421 atcaaaccaa ttcacccatt ctttcaagct ccccatttct cgaaaatttt cgggtgaaat
      481 cgagtgctta ctaatcagat ttgccaactg cgatatccga acagtaggaa aattcattgg
      541 gcgaagacga aggaacttcc ataagtgctt ctcgattggc tttagtttgt gtttccgagc
      601 taaaaacctg tattcattcc acaaagcttg tagatattca tcttcatgtt ctgaatgtaa
      661 tagtccgctt tgtccgtata acaaagcctc cagttgaaat tgactttgct tataatttag
      721 aagtatacga tatggcgttg atcgggctaa caattcaaat ggtactgcat ttaactgcat
      781 tccaaccgac cgaaaaatga aacaataaag tgcttcggtc caatcgtttt ttgtcaaact
      841 tagaagttga cgaattcgag aagatttctc ttccaaacgt tcagtaacca agcgatcaag
      901 ccagttttgc atggttataa catcaggcct acccaaagct gcacaaggaa tcgggaacat
      961 acttcctgat aactcttccc aacgttgaag tagtgattcc ttaataaatg acttcagttc
     1021 aattacaggt attacttgat tgttcaagtc aaacacatcc atatcgtgaa ttgctaccac
     1081 atgtaaaata acggtgtgat aagctgcgtc agtctcgtgt ttatgcctgt accaatccga
     1141 cgatcgtatg tgtagttcaa catttccagc ccaaagtgta tttccaattt taattcgagc
     1201 attctgaaaa tcaggacctg aatgtttatt atgtgtacct ggagaaatga cttccaaaga
     1261 ttcaccagaa gccaaagcca gcttgtctgt ttggtacaat ctgaatttcc agataaaatg
     1321 caaaagttgc tcactaatca taggaggatt ttgaaccctc aagttgagag caatccccta
     1381 aaatcatttt gctcctcata caaatgattt gcatgtcatg caaaaagctg aactaaaaag
     1441 tgtcgattta ccaacaatgg cgttgataat tagccgttta taaacgttta atatacgact
     1501 taggtccgcg aaaatgccgt tattaacatc ggagggaggt tagcggtggc cccgggctta
     1561 agtgggattt ttagtttaga aaatacttgc aatcgattga ttatctggaa acaaaaccac
     1621 aatcctgaaa tttcaacatt tcatctccaa atataaattc gactaattct tccgcttaaa
     1681 acctcaatcc gtcaaatccc ttctaaaagc gatttcatct ctaactgcat cgattgagct
     1741 tcaacagctg cggcttctcg aaaatcaata ccagatgaag catataaaat gcttctagaa
     1801 gcattgacta atatcccaac atcctttgtc aaagaagctt tgctaacatc ctttaaattt
     1861 ccgccttgag ctcctacacc tggaattaac aagaaatgat cagggactaa acttctaaca
     1921 tgctgcaatt cttctacacg agttgctcca gcaacaaaca tgagttgttc tgaacttccc
     1981 cattctgagg ctttggataa aacttcctcg aatacatatt tgccgcttgt tgtttttaac
     2041 tgctcaaaat cttccgctcc agaattactc gtaagagcaa gcagaattgt ccatttatct
     2101 tttcgatcta gaaatggctg aaccgaatct ttccccatat atggagcaac agtcacgcta
     2161 tcaaacgaat aggtatcgaa aaaagcacga gcatacatgg cagaagtatt cccaatatca
     2221 cctcgttttg catctgcaat ggtaaagtga gtgctgggaa tatactccaa tgttttcctt
     2281 aagctctccc atccagcagc acccatcgat tcatagaaag caatattcgg tttgtatgct
     2341 acacagtatt ccttcgtagc atcaataatt gctttattaa attcaaaaac ggggtcagct
     2401 tcgtttaata aatgggaagg aatttttgtg atgtccgtat caagcccaac acagagataa
     2461 cttttttgct tccaaataaa ggatattaac tccgatcggg tcataacgat tctgttcctt
     2521 cttgcatacg ttctgcattt tctgcaacct gtaatgcatt gattaattcc tcaatatctc
     2581 caccaacaaa agcaggaaga ttatacatgg taaagccaat acgatggtct gttacgcgac
     2641 tttgtgggta attatacgtt ctaatttttg ccgaacgatc cccagtagaa accatggttt
     2701 tacgtctttt cgaaatggca ttgttgtgtt tggtaagttc taaatcataa agcttagctc
     2761 gaagcatatt catagctcgg attttatttc caagctggga acgttctatt tgacacataa
     2821 ccataatacc tgttggttta tgcgtcaact ggactttggt ttctacttta ttcacgttct
     2881 gtccacctgc tccacctgag cgtgatgttt gaaattcaag atccgctgga ttgattacca
     2941 catctacttc atctgcttca ggaagcacga ctacagtagc agcagaagta tgcacacgac
     3001 cttgtgtttc ggtagcaggt acacgttgaa ctcggtgtac gcccgattcg tatttcaata
     3061 caccaaaaac attgtcaccg cttacttcca tcacaatttc cttgaatcca ccgagtgtgc
     3121 cttcactctg gtcgatcaca tccactttcc atcctttatt ctcacannnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnncgag tgtgccttca ctctggtcga tcacatccac
     3301 tttccatcct ttattctcac agaacttaat gtacatgcga gctaaatcac cagcaaagat
     3361 gctagcttca tctccacctg tacccgcacg aatttctaaa acagcattct taccatcctc
     3421 gggatctttc gggattagaa gcaagcgaat cgtttcttcc agagcgattt gttcagcttc
     3481 cagtagattg atttcatctt tagccatggc acgaaaagcc tcatccgcct cagcatacat
     3541 gagttcattc gccgatttca aatcttctaa aatcttttta taacgaatgc cactttcaac
     3601 aactggctga aggtctttgt attccttatt aagtcgtttg aaccgtgtca tatccgagat
     3661 cactgaagga tcggaaattt gaagttcgac ttcttgatat cgggctcgga ttgcctccag
     3721 tcttgggagc agggtatttt cttccattgg cggcaaaatt aggtttttta agcctcgtaa
     3781 tgaaagttcc cttgaactgt ttgtatggtt aaggctttct tgctagatga ctcaacgcga
     3841 ccaatgattt ttgcttcaac tccgaaggaa tgagaaatct cgatgattga ctcagctatc
     3901 tcactcggaa cgtacaactc catccgatga cccatattga aaacacggta catttctttc
     3961 caatcggttc ctgtcgattc ttgaataatt tgaaacaatg gaggaacgtc aaaaagcgaa
     4021 tcttttatga tatgaagttg atcgataaaa tgaagcactt tcgtttgacc accaccactg
     4081 caatgcacca ttccatgtat ttggggacga tgtttttcta gtatttgttg gatgataggt
     4141 gcataagttc gagtcggaga caacaccaat tgtccataat taaccgataa acctggcacc
     4201 tgatcggtaa gcaggtattt gccgctataa actaattctt caggtacttc aggattatag
     4261 ctttcggggt atttagatcg gtaaacagat gaaaatacat cgtggcgggc tgaagttaag
     4321 ccgttgcttc ccattccacc attgtatcga tcctcataag tagcttgacc agaagatgat
     4381 aatccaacaa taacatctcc tgcttgaatg cggctattat caatgatatc acttcgtttg
     4441 atgcgagcaa ctacggtaga atcaacaatc aaagtccgaa caatgtcacc tacatctgcc
     4501 gtttctccgc cagttaaatg aacgtgtatt ccaaaagaac taaaccaatc acatagttct
     4561 tgattcgctt ggatgatagc tgaaataact tctccgggaa tatttgattt atttcgacct
     4621 atggtggaag aaactaaaat ttgatccgta gcaccaacac ataaaagatc atcagtattc
     4681 atgaccaatg catcttgagc tatgcctttc caaacggata agtctccggt ttctttccaa
     4741 tagatatatg caagtgatga ttttgttcca gccccatcag catgcataac aagagcatat
     4801 tctggatcac ctgttaaggt atcaggaata atcttgcaga atgcttttgg aaagaggcct
     4861 ttatcggagt ttcggatggc agcatggact tcttcttttc cagaagatac gcctcgtgca
     4921 gcataacgat catgagtttg catgggtaca aaataacaac atcccgccgt ttcgggcagg
     4981 atgttgcaaa attatatttt caaaagaatt attttttcgg tttagctggt actgctggtg
     5041 ctggcgctgg tttagccgga gctggtgctg gagcagcagc tggagttggt ttagcaggag
     5101 ctggttctgc aggagcagct ggtggcactg gtgctgattt tggatcaacc tgaaggttat
     5161 cacgtttgat ctcttctaca tcagtttcaa tttcagtatc ctcatcaact gactcgatct
     5221 taggagcttt aaattcaggt gagccaactg caggaacgaa agtcgtacta gtaatacgga
     5281 attcggtacg acggttaact tgatgttctt cttcagaaca tttaactccg tctttacaac
     5341 gattcaacaa tttagtctca ccatatcctt tagctgtcaa acgagatgga tcaatctttt
     5401 gctcgattaa gtaatcaaca accgattgag cacgtttttg agaaagaaca atattcgctt
     5461 tggcagcatc acgtgagtcg gtgtgtgaca tcaactcgat tgtgatgttt gagttgtctt
     5521 tcagggtttg aaccaatcca ttcaaagaat ctttagattc agggcgaaga tcccatttac
     5581 ctaagtcgta ctcaatttta ggaagtttaa ttggaccttt cttggttgtt ttcaagaaga
     5641 aatctttttt gtagtcgcgt ccagcagttt ctccaactgt cgtttcagtt cctttatcgt
     5701 ttaagaaatc tgtagcgctt gcaagtagtt cgtatgtatt gtcttcacgg aacttcgtat
     5761 tgtcgaaata gtaagaaccg gtttgatcag tgatagcatc gtaagtcata ccatctttat
     5821 caaccatcgt aactttcgct ccagtaacag gagttttgtt atcaaaatca tatacatatc
     5881 cacccaaagt gaataagatt ggaggaacac tgaattcgta gatatcagca ccaccacgac
     5941 cacctaaacg gcttgaagag aagtagcctc tttcttgctt tccttggaag atgataccaa
     6001 aatcatctgc ttcagaattc aatgggaatt tcaagttaga aacagatgac catttacctt
     6061 ttacaaactt agcttcgaat aaatctaaac cacccattcc aactaaacca ttagaagaga
     6121 agtacaaagt tgaatcgtta cgaagtgttg gataaagttc atccccaatt gtattcactt
     6181 gaggcccaag atttacagga cttccccaag cttttgactt cttatcgaat ttagccatcc
     6241 agatatcacg tccaccaagt ccgcctggca tatcagaagc aaaatataat gtttgctcat
     6301 catcactaat ccaaggatga ccaacagtaa agctatcagc agcaaaaggc actaggattg
     6361 gttcatccca gttatttcct tttttacgtg ttgtaaagat ctgacaacct ttgattttat
     6421 ttttatcgta agaacaacgg gtgaagaaca ttacactgaa cttatcattc aaagaagaag
     6481 ctccatcatt gtattttgaa ttcaaaggct ctttgaaaga agttggagta ctccattttc
     6541 cgtttttatc caatttagct tcgaataaat cagagaatgc ttcacctgtc caaccatcag
     6601 ttccacttcc tgtagaacct tcacgactag atgtgaagat gatttcttta aaattcttac
     6661 gagaataaat tggggagaaa tcatcaaact tagagttcaa tggttgaaca ttgctaacct
     6721 catgtttagt aggagctgct ttccatttaa cagataattt acagctttca acaccagatg
     6781 taccgcgagg atcttctggt ttaagcttta catatttatc gtattctgct aaagcctcat
     6841 catatttagc gttttcacgc atcatatcag ctaaaaacaa aatagcgatt ggatcttgat
     6901 actttgcttt gattgccttt gcaaaccaaa cttccgcttt tttagtatcc ttaaatgcca
     6961 tatagcataa ggcttgttta taagtcacct cagcttttaa agctttgttt ccacgaatgt
     7021 tttcagatgc tttcttgtag gcatccaaag cttcatagaa ttctttgttg ttaaaaaggc
     7081 gatcggcttc ttttacacgt ttcggttgag caaacagcgt tgaagccgat aaagccagca
     7141 ttagcaggca tgtaaggagt tgattaaagg tctttaccat aatggagctt agttgcaata
     7201 tgcagctggc taaattaaaa aaaatgtgct tggtacaaaa aagggtggct ttctttctaa
     7261 aacaaggtga aaaacgcaaa aaaaagcgga ctaaaaagta attctagtcc gcttttcact
     7321 tgagaaaaaa ccaatctaat tagttgataa acaacatctc gcgatactta ggcaagcacc
     7381 aaatttcatc gtcaaccaaa agttcaagtt tgtcaacatg gtatcgaatt ggatcaaaaa
     7441 agccttgcac ttttgtacaa tatgcagtag caagcttcct tacatcagca attttgtttg
     7501 cttgcttgcg cgattcgatc atttcatcca ccattttctt gatcgcattc acatgttcag
     7561 aaatttcagt aattatttcc aattgtgctt gggctgattt ctgaaatgtc tttgcatcca
     7621 tgatatcttt caacccttta acattctgta tgagagcact ttgatatttc aaagccgttg
     7681 gaacaatatg attagcagct aaatctccca tcacgcgtga ttcaatctga attttcttca
     7741 catacatctc caactgaatc tcataacgag cctccacctc gcgatgactc aacacatcat
     7801 tttcttcaaa taacttcaaa gatggtttac taacaaaagc atctaatgct tctggtgttt
     7861 tggcaaagtt attcaagcct cgcttagctg cttctttaac ccattcatca ctatatccat
     7921 tcccttcaaa tcgaattgct ttagattcag taatgtagcg acgtaaaact tggaaaatcg
     7981 cttcgtcttt atccagcttc tttttagcaa ttaagccatc tacttcaatt ttgaaatcac
     8041 gtaactgttg agttacaatg gtgttcaata caatcatcga ttgcgaacaa ttagctgaag
     8101 atccaactgc acggaattca aatttatttc ccgtaaaggc aaaaggtgaa gtccggttac
     8161 gatcggtatt gtcgagcaaa atctgaggaa tcttaccaat attcagtttc aaatcagtct
     8221 tttcatctgg agtcatctta ccagatgctt tcacacgttg ctctaaatca tccaaaacac
     8281 cagtaagctg agaaccaata aaagcagaga taatagctgg aggagcttca ttagcaccca
     8341 aacgatgatc attaccagct gacgcaatag cagcacgcat caaatcggca taacgatgta
     8401 atgctttgat agttgtaatg aagaatgtta ggaattgtaa attgttttta ggagtttttc
     8461 ctggacttaa taagttcttt cccgtattgg tagaaagtga ccagttatta tgcttcccag
     8521 aaccattgat tcctgcgaat ggtttttcgt gaagtaaaat gcggaaacga tggcgacgag
     8581 caactttact catcacatcc atgagtaatt gattatgatc aacggctaag tttgcttctt
     8641 caaatatggg tgcgcactca aattgattag gagctacttc attatgacgc gtacgaaccg
     8701 ggattccgag tttatgacat tctgtttcga aatcgcgcat ataagctgcc acacgatcag
     8761 gaatagttcc gaaataatga tcttctaatt gttgaccttt cgctggtgaa tgcccaaata
     8821 gtgtacgacc cgttaaagat aagtctggac gagcatggaa aagtgcttca tcgattaaga
     8881 aatactcctg ttcccaacca agtgtagcag ttacttttgt aacgtttttg tcgaagtatt
     8941 gacaaacatc aactgcagcc ttatctaaag cgtgtaaggc tttaattaat ggggttttga
     9001 aatcgagcgc ttctcctgtg taggatacga aaatggtagg aatacaaagt gttccgctaa
     9061 tgataaaagc aggagatgta ggatcccaag ctgtataacc tcgagcttca aacgtattac
     9121 gtattccacc acttgggaat gatgaagcat ctggctcttg ttgaattaag ttgctaccac
     9181 taaaattctc aattgcacga ccatctgcta aaggctcaaa aaatgaatca tgcttctctg
     9241 ctgtagctcc gttcaaaggc tgaaaccaat gtgtgaaatg cgtacaaccc atgttcattg
     9301 cccaagcttt catcgaagag gcaacttgat ctgccacatg acgttcgatt tgagttcctt
     9361 gttcaattgc cccacgcact ttatcgtagg catctttact gaggaattca cgcatggaaa
     9421 tatccccaaa tacatgaaca ccataataat cagagatttt ctctgctgat ggaccattcg
     9481 gctcatacgt ccgattaatc aaagactcta atgctttaaa tcgaatagtt gacatggaaa
     9541 tatttttatg tttttcggga gcaaatataa attttctagg gggtaagttg aaatattttt
     9601 gatttttttc cttaacaccc cctatttttt gggtccgact tgaaatattc ttgctttttt
     9661 caaattttcg aaagtggaat gaagcaaacc cattttgaga attcagacat ccaaacagg
//
LOCUS       DAOOQW010000073         9587 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_8846_length_9587_cov_4.381872, whole genome shotgun sequence.
ACCESSION   DAOOQW010000073 DAOOQW010000000
VERSION     DAOOQW010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 9587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9587
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8846_length_9587_cov_4.381872"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="PK281_05860"
     CDS             complement(<1..184)
                     /locus_tag="PK281_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HOY48904.1"
                     /translation="MEINLTTPALLFPAISLLLLAYTNRFLAIANLIRGLKKQYENEH
                     DTNVLDQIKNLQKRILL"
     gene            295..2661
                     /locus_tag="PK281_05865"
     CDS             295..2661
                     /locus_tag="PK281_05865"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY48905.1"
                     /translation="MQHRYAYRSGILNSFKLLLLVCLSTSFSLVNAQIYELEGLNLPG
                     QWNNFVNPPATGSVFGSSTQALGTVHPINTGTRRWQTRIHIAASGADTSAGSYSFLFT
                     SGPNATPYANKWAGVNVIIDSLQSYQFNAGADNSITVQNDKVYTMNWEDRGYQANRAI
                     FMETSNEPVSLISQSTNIIGTTVGPQTPVNATLLLSAIPSPEEKFYLRYSTDFYATSF
                     IIPVSVVGTTGTATIPGFAAGLAVSYYYFSTTIDNPTANYDLLTLDFLNDDGYNFLYG
                     VVDPFLSFGLGDDILACPGSIQDTLQTAGTYDTYLWSDSSTASTLVINSPGTYWLEAS
                     IGSEIHRDSITISELTLSPIDLGESGTICGGVPLELFPGVKISPLGDSLTIIYDATRG
                     QSTLTGATSVYMHSSFEYAPLSGAVEPWVGNWGVNDGLGEMTNIGEDLWSITINVYDY
                     YGINPDSSVNGLFIVFRNATGTATGKDENGNDIFLGLYQNPPYSAFTGIGGSIESSGY
                     DEILWSTGETSPSIQVSEAGTYWVNMSSYLGCSLSDTITVITSPNPSINLGADRTSCN
                     NQPVTIYATGGFSSYYWSNGTTTQALTTAVAGTYEITVTNSAGCSASDLISVLENVTP
                     IASFSTVQSGLTVNITNESTLTATYAWDFQNNGSVDNTNVGDVSFTYPAPGQYLIRLI
                     VSNYCGADTLFKSVIVTDVGIEQVAAQSSLVVYPNPTTGNLNIQSKSKDATLRIHSSS
                     GLLIGEINAEEINLSGWNILQDKAPGLYILQEISSSGTRICPIILIKE"
     gene            complement(2696..3448)
                     /locus_tag="PK281_05870"
     CDS             complement(2696..3448)
                     /locus_tag="PK281_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOY48906.1"
                     /translation="MPTAVTRTAQLNPHVSVDCVIFGFDEGELKVLLIERDLDITDQS
                     TRKFSLPGNLIDDNEDLDTSAKRILKELTSLDNIFLEQFYAFGDPNRVKNSSDAQWLN
                     AVRDVPTARVITVAYYSLVKIGDYQPSAASFARKAFWCPVSQVPELAFDHNQIFNQAL
                     LSLKTKLRMQPIGFELLPDKFSLGQLQKVYETILGTTLDKRNFRRKILNKGFLKALQE
                     KQKGVPHKRARLFEFDKEAYDRLKADQFYFEF"
     gene            3666..4646
                     /gene="pfkA"
                     /locus_tag="PK281_05875"
     CDS             3666..4646
                     /gene="pfkA"
                     /locus_tag="PK281_05875"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010228448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOY48907.1"
                     /translation="MSKIQRIGIFTSGGDAPGMNAAVRAVVRACIYYKLTPVGIYRGY
                     DGMISGDIKVLDSRSVSNIIQRGGTILKTARSMEFMTPEGRKKAYDNLREFEVDALVA
                     IGGDGTFRGAEVFSNEYGIPVVGLPGTIDNDVNGTDFTIGYDTAVNTACAAIDKVRDT
                     ASSHNRLFFIEVMGRDSGMIAMHSSIAAGAEATMIPERPLDIDGLIKLLEIGAVGKKS
                     SSLVVVAEAGKPGRSMEIAKQVSERFAHYDTKVTILGHVQRGGSPTVADRVLASRMGV
                     VAVESLLEGKSGVMIGIVNNQVKFTAFDQATKNVNNFENELLRIAAILSV"
     gene            4655..6277
                     /locus_tag="PK281_05880"
     CDS             4655..6277
                     /locus_tag="PK281_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007840177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HOY48908.1"
                     /translation="MLHTGSYIDLFPHLNQVPTGLQPEVLDQRVFLCNGELKPWTGKQ
                     TDVHSPIYIQSGDSFEQIRLGSYPEMGPDDMQQVLDSALKAYNLGRGEWPTLKVRDRI
                     AAIEHFLRQILEKRELIVRWLMFEIGKNQSDSEVEFDRTVEYIEDTLEAVKNLDRETS
                     RLELHHGIYAQVRRGPLGVVLCMGPYNYPLNETFATLIPALIMGNTVIFRPAKFGILL
                     IHPLLEAFRTCFPPGVINVIYGEGKETAGKLMETGKIDVLAFIGSSKVANILKKQHPH
                     SNRLRSVLGLDAKNPAIILPDANLDIAVSECLNGALAFNGQRCTALKLIYVHRSIVEA
                     FLEQLCRKVELLKAGMPWEKDAYITPLPEPGKPAYLQELVDDAIAKGASILNEGGGRS
                     HLSFYFPTVLFPVNNTMRIYQEEQFGPVIPVIAFDSVDEPVEYIVQSHYGQQVSIFSN
                     DADEIAQLIDPLINQVCRININTKCQRGPDNYPFNGRKDSAEGTLSVTDALRVFSIRT
                     MVATRDTEENRHVFTQILEGRKSNFLSTDFIL"
     gene            6296..7159
                     /locus_tag="PK281_05885"
     CDS             6296..7159
                     /locus_tag="PK281_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HOY48909.1"
                     /translation="MLLIADSGSTKCDWMLLKENEEAQSFSTMGFNPYFHNEAVISNA
                     IRQNTGLCELAPQVTMLFLYSAGCSSKELKLVVERALRSVFVNANIYVDHDLVGAAFA
                     TWDENPAITGILGTGSNSCYFDGDIVKQANSSLGLGYILGDEGSGSYYGKILCKKFLS
                     NSLPDEIHNVLANDFKLSRDIIVENVYMKPHANVYLASFMKVLSDNKQLDWVQKLVDK
                     GMEDFFTSSIACYKNYQRVPVHLIGSIAYHFEDNVRRVGSNMGIQVGTIIKKPIYGLV
                     NYHMKQHFAKK"
     gene            7177..>9587
                     /locus_tag="PK281_05890"
     CDS             7177..>9587
                     /locus_tag="PK281_05890"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HOY48910.1"
                     /translation="MALQHRQGGTFNEAVSDYLSHSVTNQEITFNTSNAHIRVSVYDH
                     AIIRVRIAKNDSNFSGFSYAVINEPQATPFDFSESATGFSLKTQALEIRISKVPFRIS
                     AYTLDGKILTADDPAFGTSWLANEVTTYKQLQPDERFIGLGEKTGNLDRRGKTYVNWN
                     TDNFAYPTDGDPLYLSTPFYMGIVEGRPYGIFFDNTHKTTFNFGCSNDRFSYFQAEDG
                     EMNYYIIHQPTVAGIIERYTWLTGRMEMPPLWSLGFQQCRYSYYPYPEVLNAARTFRE
                     KDIPADVLYLDIHYMEAYKVFTWHPTRFPDPKGLLKELKEMGFNVVVILDPGIKKETG
                     YHAYDEGKKLDMFVKYPDGAEYNGQVWPGWSAFPDFTKEEARTWWAEKMHTITDVGVE
                     GFWNDMNEPAAWGQHLPDLIEFDYEGEGATHKKARNVYGMQMARSTQEGGKNLLKGKR
                     PFVLTRAGYSGIQRHAAVWTGDNVSTDDHMLAGVRLVNSMGLTGVAFAGYDVGGFAGE
                     ATPELFARWIALGAFSPFFRSHSMVNSRDSEPWSFGEAVEDVSRNYIKLRYRLMPYIY
                     SSFYETSQTGIPVARSLAIDYTNDAKIYRNPYQNQYLFGASILVIPCESTQLHHKAYF
                     PEGSWYNLYTDAVQAGGVEVVLEAGMETLPLYVKAGSILLMQDSVTSLMKEKAAETLE
                     IHVYPGASTRFVHYEDDGATYEYAQGKSSTREFVLADKSLNIGAASGNFNSPIKTWKI
                     YLHGQAGNTSKLLVNGKEMTASSQNYRFIEPISDFDPYHVPPQDPIQVKNLPYIELPS
                     QSGEV"
BASE COUNT         2801 a         1984 c         2000 g         2802 t
ORIGIN      
        1 ttagtaagat acgtttttgt agattcttaa tttgatctaa cacatttgta tcgtgttcgt
       61 tttcatactg ttttttcaag ccacgaatta gattagctat agccaaaaat cggttcgtat
      121 aggccagcaa caacaaggat atcgctggaa agagcagggc aggagttgtg agatttattt
      181 ccataaaaat ggtgcttata cttgtagaat ctattggatt gaatgcaaat agatatgtgt
      241 ttaaagtgta tttttgacac tcaatcgttt taataaccct aaactacgga gtttatgcaa
      301 caccgttacg cgtatcgttc aggaattctg aacagtttca aactgctttt attggtttgt
      361 ttgagcacat ccttttcatt ggtgaatgct cagatttacg aactcgaagg attaaatctt
      421 cccggacaat ggaacaattt tgttaatcca cctgcaacag gatcggtttt cggaagttca
      481 acacaagctt taggtaccgt tcatccaatc aacactggca cacgtcgctg gcaaacccgc
      541 attcacattg cagcttcagg tgctgatact tcagcaggaa gctatagttt cctttttact
      601 tcaggaccaa atgctactcc gtatgctaac aaatgggcag gcgtaaacgt catcatcgat
      661 tcgttgcaat cgtatcaatt caatgctggt gctgacaata gcatcacggt tcaaaacgat
      721 aaagtttaca ccatgaattg ggaagatcga ggctatcaag ccaaccgagc cattttcatg
      781 gaaacaagca acgaaccagt ttcgcttatt agtcaatcaa ccaacataat cggaacaaca
      841 gtaggcccgc aaactcctgt taatgcaact cttctactta gtgcaatacc ttcacccgaa
      901 gagaaatttt atttacgcta cagcactgat ttttacgcaa caagctttat cattcctgtt
      961 tcggttgttg gtactacggg aacagctacc atcccgggct ttgcagctgg attagctgtt
     1021 tcatattatt acttttcaac cacaatcgac aatccaactg ctaactacga tttacttaca
     1081 ctcgatttcc taaacgatga tgggtataat tttctatacg gtgtggtaga tccatttctt
     1141 tcttttggtt tgggtgatga tattcttgct tgtccgggaa gcattcaaga taccttgcaa
     1201 actgccggaa catacgatac ctatttatgg tcggatagtt caactgcttc tactttagtc
     1261 atcaactcgc ccggtacata ttggttagaa gcttctattg gttccgaaat tcatcgagat
     1321 tccatcacta tttctgaatt aaccttatcc ccaatcgact taggcgaatc aggaaccatt
     1381 tgcggtggtg ttccattgga attgtttccg ggtgtgaaaa tttctccttt aggtgattca
     1441 ctcacgataa tttacgatgc cacgcgagga caatctactt taacaggtgc tactagtgtt
     1501 tacatgcatt ctagttttga atatgctcca cttagtggtg ctgtagagcc atgggttgga
     1561 aactggggag taaacgatgg tttaggagaa atgaccaaca ttggagaaga tctttggtca
     1621 ataacaatca acgtttatga ttactacggc ataaatccag attcaagtgt gaatggttta
     1681 tttatcgtgt ttcgaaatgc tactggaaca gcaaccggaa aagatgaaaa cgggaacgac
     1741 atctttcttg gactttatca aaatccgcct tactcagcat ttacaggtat tggcggttca
     1801 attgagtcaa gtggatatga cgaaatactt tggtcaacag gtgaaacaag tccaagtatt
     1861 caagttagcg aagcaggaac ttattgggta aatatgagca gctatttggg ctgttcgttg
     1921 agcgatacca ttactgtgat tacttcacct aatccaagca ttaacctagg tgccgataga
     1981 acgagttgta acaatcaacc tgttacgatt tatgctaccg gaggatttag ttcctattat
     2041 tggtcaaacg gcacaactac acaagccctc actactgcag tagcaggaac atatgaaata
     2101 acagttacca actccgccgg ttgttctgcc tcagatctca tttcggtgct tgaaaatgta
     2161 actcctatag catcattctc tactgttcaa tcaggcttaa ccgtgaatat taccaatgaa
     2221 agtacgctaa cagcaacgta tgcttgggac tttcaaaata acggatcggt tgataataca
     2281 aatgttggtg atgtgagctt tacttatcca gctcccggac aatatcttat acgtttaatt
     2341 gtaagtaatt actgtggtgc cgacacatta tttaaaagtg taatcgttac agatgttgga
     2401 atagagcaag tagctgctca atcaagctta gttgtttatc cgaatccaac gactggaaat
     2461 ctgaatattc aatccaaatc aaaagatgca accttgcgca tacattcatc ttcaggactt
     2521 ttaattggag aaataaatgc agaagaaatt aatcttagtg gatggaatat tttacaagac
     2581 aaagcaccag gcttatacat cctgcaagaa ataagttctt caggcactcg aatatgcccg
     2641 ataatactga ttaaggaata aaaaaatccc cggctgaatg agtcggggat tttttttaaa
     2701 actcaaagta aaattgatct gctttgagtc ggtcgtatgc ttctttatca aattcaaaaa
     2761 gccgtgctcg tttgtgagga acgcctttct gcttttcttg taaagcctta aggaatcctt
     2821 tgtttaggat tttccgacgg aagtttcgtt tatcgagcgt tgtacctaaa atggtttcat
     2881 aaaccttctg caattgtccc aaactgaatt tatcgggtaa caattcaaat ccaatgggtt
     2941 gcattctcag ttttgttttc agactcaata atgcctgatt gaaaatctga ttgtggtcga
     3001 aagctaattc aggtacctga gaaactggac accaaaatgc tttccgggca aatgatgctg
     3061 cagatggttg ataatcacca atcttaacca aagaataata ggcaaccgta attacacgag
     3121 cagtaggaac atcccgaaca gcatttagcc attgagcatc agaactgttc ttaacccggt
     3181 tcggatctcc aaaagcataa aactgctcca agaagatatt atccaaactg gtgagctctt
     3241 ttaatattcg tttagctgaa gtgtctaagt cttcattatc gtcaatcaag ttacccggta
     3301 aagaaaattt acgagtactc tgatccgtga tgtcaaggtc gcgttcgatc agcaacactt
     3361 tcagttctcc ttcgtcaaat ccaaatatga cgcagtcaac agaaacgtgt ggattaagct
     3421 gagcggtacg agttacggcc gtcggcatag ggtgtagcaa attcattgaa aaggtgcgta
     3481 aatatactgt tttatgatga attgtgtcta ttgtacacta ctatgctgca ttcctacata
     3541 attaaaggct ctgtcgcttt tttcaccatc ttgcaagccc taatttcaat acgcgtgttg
     3601 ataattttag ctaggataaa aactaagtgt cattttaaca cttattttag ccctttattt
     3661 cgactatgtc caaaattcaa cgtataggta tttttacatc tggtggagat gctcccggta
     3721 tgaatgccgc agttcgtgca gttgttagag cttgtattta ctataaactc acaccagttg
     3781 gtatttatcg cgggtatgat ggaatgattt cgggcgatat caaagtgctc gatagtcgtt
     3841 cggtttccaa tatcatccaa cggggcggaa caatcctcaa aacagcccgc agcatggagt
     3901 ttatgactcc tgaaggaagg aaaaaagcgt acgataatct tcgcgaattt gaagttgatg
     3961 cccttgttgc cattggtggt gacggcacat ttcgcggggc ggaagttttc tccaatgaat
     4021 acggaattcc agtcgttggc ttgccaggta caatcgataa cgacgttaat ggtactgatt
     4081 tcaccatcgg atatgacacc gcagtaaata ccgcttgtgc agccatcgat aaagtgcgcg
     4141 atacagcatc atcgcacaat cgcttgttct ttatcgaagt gatgggacgt gattcgggaa
     4201 tgattgccat gcatagctcc attgctgctg gcgctgaagc aaccatgatt cctgaacgac
     4261 cactcgatat cgatggactt attaaattac ttgaaatagg agcagttggt aaaaaatctt
     4321 ccagtctagt tgtcgttgcc gaagctggaa aaccgggtcg atccatggaa atcgccaaac
     4381 aagtttctga acgttttgca cattatgata ccaaagttac catactcgga cacgttcaac
     4441 gtggcggttc gcctacagtt gccgatcgtg tattagcaag tagaatgggt gttgtagctg
     4501 ttgaaagtct gttagaaggc aaatcgggtg tgatgatagg cattgtaaat aatcaagtta
     4561 aatttacggc cttcgatcag gctactaaaa acgtaaataa cttcgaaaac gaactgctgc
     4621 gtattgctgc gattctttcc gtttgataca aaacatgctg cataccggct catatataga
     4681 tctttttcct catttaaatc aagtaccaac cggacttcaa cccgaggtgc tcgatcaacg
     4741 tgtatttcta tgcaatggtg agctaaaacc atggaccggt aagcaaacag atgtgcattc
     4801 acccatctac attcaatcag gtgattcgtt cgaacaaata cgcttaggct cttatcccga
     4861 aatgggtccg gatgacatgc aacaagtgct cgattcggcg cttaaagcat ataatttagg
     4921 aagaggtgaa tggcctacgc taaaagtccg tgatcgaatt gctgctattg aacatttcct
     4981 tcgtcagata ctcgaaaaac gtgagcttat tgttcgttgg ctgatgttcg aaattggtaa
     5041 aaatcaaagt gattcagaag tagaattcga tcgaacagtt gaatacatag aagataccct
     5101 agaagctgtc aaaaatctcg atcgcgaaac ctcacggctc gagttacatc atggtattta
     5161 cgcccaagtt cgacgaggtc ctctaggtgt tgtactatgt atgggacctt ataattatcc
     5221 actaaacgaa acgttcgcca cgctaattcc cgcgttaatc atgggaaata cggtgatttt
     5281 tcgtccggct aaatttggaa tccttctcat ccacccttta cttgaagctt tcagaacgtg
     5341 ttttccaccc ggtgtcatca atgtgattta tggcgaaggc aaagaaaccg ctggaaaact
     5401 catggaaaca ggcaagattg atgtgctagc ctttattggt tctagcaaag ttgccaacat
     5461 cctcaaaaaa caacatcctc attccaatcg cttgcgttcg gttctcggat tggatgcaaa
     5521 aaatccagca atcattcttc ccgatgctaa tttggacatt gccgtatctg aatgtctaaa
     5581 tggagctcta gcatttaacg gtcaacgttg tacagcactc aagttaattt atgttcatcg
     5641 ctctattgtc gaagcttttc tcgaacaact gtgtcgtaaa gtagagctgc ttaaagctgg
     5701 aatgccttgg gaaaaagatg catacatcac accacttccc gaaccgggaa aacctgccta
     5761 tttgcaagaa ctagttgatg atgccattgc taaaggtgct agcatcctga acgaaggcgg
     5821 aggaagaagt caccttagtt tttactttcc taccgtgctt ttccctgtca ataataccat
     5881 gcgtatttat caggaagaac agtttggacc tgtcattcca gtaattgcat tcgattcggt
     5941 agatgaaccg gtggaataca tagtacaaag ccactacgga caacaagtta gtatcttcag
     6001 taatgatgcc gatgaaatcg cccaattgat tgatccactg attaatcagg tttgtcggat
     6061 taacatcaat accaaatgtc agcgtggtcc cgataactat ccatttaacg gacgaaaaga
     6121 ctcagctgaa ggtactttaa gtgtgaccga tgcgctacgg gttttctcta ttcgtactat
     6181 ggttgcaact cgcgacaccg aagaaaatcg tcacgttttt actcaaattc tcgaaggcag
     6241 aaaatctaac tttttgtcga ccgattttat actttgaacc taaatacatt aacctatgct
     6301 actcattgcc gatagcggat ctactaagtg cgactggatg cttctcaaag aaaacgagga
     6361 agctcaaagc tttagcacca tgggattcaa tccttatttc cacaacgaag cggttatctc
     6421 caatgccatc cgccagaata ctggtttatg tgaattggct ccgcaagtaa ccatgttgtt
     6481 tttatacagt gcaggttgct ccagtaaaga gttaaaactg gttgttgaac gtgctttacg
     6541 ttcggttttc gtgaatgcaa atatctatgt cgaccacgat ctagtcggtg ctgctttcgc
     6601 tacttgggat gaaaatccag ctattaccgg aattcttggt acaggttcca attcctgcta
     6661 cttcgatggt gatattgtga aacaagccaa tagctcactc ggcctaggtt atatactagg
     6721 tgacgaggga agcggttctt actacggtaa aatcctgtgt aaaaagttcc tttccaatag
     6781 tttacccgat gaaatccaca atgttctcgc gaacgatttc aagttaagtc gcgacatcat
     6841 cgtcgaaaac gtatacatga aaccacatgc aaacgtttat ctagcctcat ttatgaaggt
     6901 tttatccgac aataaacaat tggattgggt tcagaaatta gtggataaag gcatggagga
     6961 tttctttacg agcagcattg cttgttacaa gaattaccaa cgtgttccag ttcatttgat
     7021 tggttcaatt gcctatcatt tcgaagataa tgtgcgccgt gttggatcaa acatgggcat
     7081 tcaagtagga acgattatca aaaaacctat ttacggtttg gtgaactacc acatgaaaca
     7141 acattttgct aaaaaataag ctaagaaatc acatacatgg cgctacagca ccgtcaagga
     7201 ggaactttta acgaggccgt ttccgactat ttaagtcatt cggttacgaa tcaggaaatc
     7261 acattcaata ccagcaatgc tcacattcgt gtaagcgttt acgatcacgc aatcattcga
     7321 gttcggatag ctaagaacga tagcaacttc agtggatttt cgtatgcagt aattaacgaa
     7381 cctcaagcta caccgttcga ttttagtgaa tctgcaacag gcttttcact taaaacacaa
     7441 gcactcgaaa tccgcatcag caaagtacct ttccgtattt ccgcttatac tttagatgga
     7501 aaaatcctca cggcagatga tcctgctttt ggtaccagct ggttagctaa cgaagtcact
     7561 acgtataaac aacttcaacc cgatgaacgc tttatcggac taggggagaa gaccggtaac
     7621 ctcgaccgcc gtggaaaaac ctacgttaac tggaacaccg ataattttgc ttatccaacc
     7681 gatggtgatc ctctttactt gagtactcca ttttatatgg gcattgtaga aggtcgtccg
     7741 tacgggattt tctttgataa tacgcacaaa accactttca acttcggttg ctcgaatgat
     7801 cgtttttcgt atttccaagc cgaagatggg gagatgaatt actacatcat ccatcaacca
     7861 acggttgcag gcattatcga acgctatact tggcttacag gcagaatgga aatgccacca
     7921 ctttggagcc ttggatttca acaatgtcgt tatagctact atccatatcc agaagtgctc
     7981 aatgcagcgc gtacgtttag agagaaagat attcctgcgg atgttcttta cctcgatatt
     8041 cactacatgg aagcctataa agtgtttact tggcatccaa ctcgtttccc tgatcctaaa
     8101 ggtttgttga aagaattgaa ggaaatggga ttcaatgtgg tggtaattct cgatccgggt
     8161 attaagaaag aaacaggata tcacgcttac gatgaaggca agaaactgga tatgttcgtg
     8221 aaatatcctg atggtgctga atacaacgga caagtttggc caggttggag tgctttcccc
     8281 gattttacga aggaagaagc acgtacttgg tgggcagaaa aaatgcacac aattactgat
     8341 gtgggcgttg aaggtttctg gaacgatatg aacgaaccag ctgcttgggg acaacatctt
     8401 cctgatctaa ttgaattcga ttacgaaggc gaaggtgcta cgcataaaaa agcccgtaac
     8461 gtttacggaa tgcaaatggc tcgcagcact caggaaggtg gtaaaaatct tctgaaagga
     8521 aaacggcctt ttgttttaac gcgtgccgga tacagcggta ttcagcgtca cgcagcggtt
     8581 tggacaggcg ataatgttag taccgatgat cacatgttag ctggcgttcg attggtgaac
     8641 agcatgggct tgacaggtgt tgcattcgct ggttacgatg ttggaggatt tgccggagaa
     8701 gctacaccag aactttttgc tcgttggatt gctttgggtg cattttcacc tttcttccgt
     8761 tcgcattcca tggtcaatag ccgcgattcg gagccttggt catttggtga agcagtagaa
     8821 gacgtgtcgc gtaattacat caagcttcgc taccgactca tgccgtatat ctattcctcg
     8881 ttttatgaaa cttcccaaac aggaattccg gtggcacgtt cgttagcaat cgattatacg
     8941 aacgatgcta aaatctatcg taatccttat cagaatcaat acttgtttgg tgcatctatt
     9001 ttggttattc cgtgtgaaag tacccaactc catcataagg catatttccc agaaggctct
     9061 tggtataatt tgtacaccga tgctgttcaa gctggtggag tagaagtagt gctagaagct
     9121 ggaatggaaa cacttccatt atatgtgaag gctggaagca ttttgttaat gcaggattct
     9181 gtgacgagtt tgatgaagga aaaagctgcc gaaacactcg aaattcatgt gtatccgggc
     9241 gcttcaacac gatttgttca ctatgaagat gatggtgcta cttatgaata tgcacaaggc
     9301 aaaagctcaa cacgtgaatt cgtgctagct gacaaatcac taaatattgg agctgcttca
     9361 ggtaacttta attctcctat caaaacctgg aaaatatacc tacacggaca agcaggtaat
     9421 acatccaaac ttcttgtgaa tggtaaggaa atgacagcta gtagtcaaaa ctaccgattc
     9481 atcgaaccta tatccgattt tgatccttat catgttcctc cgcaagatcc gattcaggtt
     9541 aaaaatctac cgtatatcga acttccttct caatcgggcg aagttca
//
LOCUS       DAOOQW010000074         9472 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_8975_length_9472_cov_5.618244, whole genome shotgun sequence.
ACCESSION   DAOOQW010000074 DAOOQW010000000
VERSION     DAOOQW010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 9472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9472
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8975_length_9472_cov_5.618244"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1622)
                     /locus_tag="PK281_05895"
     CDS             complement(<1..1622)
                     /locus_tag="PK281_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOY48911.1"
                     /translation="MKIQMLRNLLLLLLILPVTQGNLYADKGANKLQLLRIKNTMMGG
                     DYATAWRSLRDMEKTDSTTAELYFMSGECNFHLKNYEDAKERLERSIRLDAVNDPEKY
                     YFLGQTYQQMGQLSKAIVEYQHFIEKSPKKNDDTEEATALLDQCRRAVELMSKPVNVQ
                     IRNIGEAINSEYPEYNPSVSADGKTMIFTSRRPESTGKLTDPEDGKFYEDIYISVRDS
                     ITGKWLEAENIPGSLNTETHDANMTLTPDGSQIFVYRNMGYRGSGELFVSKKSRAGKW
                     SATKPVEGDVNTSYFESSACISPDGKTLYFVSERPRGGFGMGDIYMAKKKSKTEWGDA
                     VNLGSVVNDEFDQIGLFMHPDGKTLYFASNSTKSLGGYDIFKTTVENGKCTPPENLGY
                     PINTTGDERFFALSTDGRLAWFSSNREGGTGDLDIWEIDFSALQTQVKTDSSKAPVQV
                     PKGPPLSILNGKVIDSDAGQTIETEISITDKENGKVVSVSSDENGDYFITLEGERTYV
                     IEVKNALFKPYTFEVFLKGQAEGTFTKEKMIV"
     gene            complement(1658..2425)
                     /locus_tag="PK281_05900"
     CDS             complement(1658..2425)
                     /locus_tag="PK281_05900"
                     /inference="COORDINATES: protein motif:HMM:NF014658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis protein"
                     /protein_id="HOY48912.1"
                     /translation="MLRLSVVSYLNTAPFLYGLERSGLLESDSIQISLDIPAVCADKL
                     RSGMVDVGLVPVATLLSLPQYSILGNTCIGAVGKVDSVVLMSDVPLEEIEEIVLDFQS
                     RTSVNLCRLLARDYWKINPVFTSAKPDFLSTLGGKRAGVLIGDRVFDYAHRFKYVYDL
                     SEAWMNWKLQPFVFACWASINPIADEKWQKVEDALLLGISNIELAAKSVEANYPAHYP
                     VLNYLSERISYTLDSPKRAGLETFLSALSTITLLESQ"
     gene            2549..3043
                     /locus_tag="PK281_05905"
     CDS             2549..3043
                     /locus_tag="PK281_05905"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOY48913.1"
                     /translation="MAQPLLFETLPLGRHLAVLTRLYYGALTLELSDLGIDRYYSVLM
                     YIDRSEHPVTQQMVGEFLLMDKTSMVRVIDFLVEKGYVKRVTNPNDRRSYIIELTEKG
                     HEILPKIIQAVAQINDQVMEGLSEAERAQFHHTLCHISTRLKKMPAKDIQIDFQPNPN
                     NFPQ"
     gene            3047..4123
                     /locus_tag="PK281_05910"
     CDS             3047..4123
                     /locus_tag="PK281_05910"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOY48914.1"
                     /translation="MKRTLILILGIALLILIKIFFLTKKEDPSKSGPPGKGQPTPVTV
                     YIVDSSRIDNQITSVGTLRSEEEVDLQPEVSGKITSIHFREGDRVSKGQLLLQLNNAD
                     LLAQLGKLNVQRKVQEEKAGRLKSLLQMQGSSQEEYDQAQNVVASTDADIKYIQAQLA
                     KTEVRAPFSGVIGLRSVSEGAFVTANTRIARLQQTDQLKLDFNVPERYISLLELPLKV
                     AFGVQGRSDKFEANVYAIEPSVSNTTRTVQLRARVSNNAQQLLPGQFAQVLLPIKNDH
                     SSILIPTQAIIPILKGQKVFVCKQGKAAEVKVETGYRNDAMIEVLSGLSVGDSVITTG
                     IMSLKADAPLKIIPAKSQKKEVRS"
     gene            4120..7188
                     /locus_tag="PK281_05915"
     CDS             4120..7188
                     /locus_tag="PK281_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008613887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOY48915.1"
                     /translation="MSSLSTISIRRPVLAIVMNLIIMLFGWIGYTYLGVREYPSIDPP
                     VITVRTGYAGANADVIESQITEPLEKALNGIEGIRTISSASNQGSSVITVEFNLGSNL
                     EAAANDVRDKVSQAVRDLPKDIDGLPTVSKADANSDAILSLTIESNTKTQLEVSDYAE
                     NVIGERLQTIPGVSSLQIWGQKKYAMRLWMDPSRMAALGITPMDVQNALIQQNVELPG
                     GKIEGNATELSVKTVGRLRSTEEFNNMIIRNESGVNVRFSDIGEARLGPENEETILRQ
                     SGTPMVACALVPQPGANYLEIAKEFYKRVDEIKKDLPDGFKLGIALDNTRFVSQSVDE
                     VVETLLIAILLVIIIIYLFFRDWLIAIRPLIDIPVSLLGAFFIMYAMGYSINVLTLLA
                     IVLATGLVVDDGIVVTENIYKKIEGGMNPIEAAEKGSNEIFFAVISTSVTLIAVFMPV
                     IFLEGFVGRLFREFGVVLAGAVAISAFVSLTLTPMLNARLVRKDPTKKSKFYVATEPF
                     FRSMEEGYSNMLGSFMKKRKLAWLILLGAVAMIFGIFPLLQKELSPLEDRSWMRLMVS
                     APEGASYEYTDDFVLKMSDFLSDSVPEKNVILTVTAPGFAGSGAVNSGFIRLTLSPPD
                     TRKRTQQDIANYVMKKAKGFPQAKVFAIQQQTISTGGFGGLPVQFVLQAPNFERLKEV
                     LPKFLEEANKNPIFQGVDVNLKFNKPELNVSINRDKAESMGVSVQAIAQTLQLSLSGS
                     RMAYFTMNGKQYQVIGQVDRVNRDEPIDLKSLSVRTNTGELVQLDNLVDVAEVSSPPQ
                     LYHYNRYSSATVSAGLAPGKTIGDGIKAMKDISAKLLDDSFSTSLTGASRDFEESSSN
                     ILFAFLLALLLIYLVLAAQFESFRDPLVIMITVPLALAGALLSLWYFNQTLNIFSEIG
                     MIMLIGLVTKNGILIVEFANQLQERGEDKASAILNAASARLRPILMTTLATALGALPI
                     ALALGAGAVSRMGMGIVIIGGLLFSLALTLFVIPAMYSFIAKTRVPHEKN"
     gene            7175..8479
                     /locus_tag="PK281_05920"
     CDS             7175..8479
                     /locus_tag="PK281_05920"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOY48916.1"
                     /translation="MKKTNWIAVLILSCTTVLNAQEVLSPEKAVELALKNNYSILIAR
                     NEQLSSDAQVTRGNAGMLPRVDVAAGASAQSTNINQRFSNGLTVATNGVSNSASNASA
                     VLSWTLFDGGKMFLTWDRLKEQKSLNQLQLREQVEQNIAAVLDAYFLVIRLKQEVRSR
                     QFALDVTEEQFNLTRKKVELGSSSRQELLQVTVDRNTALSQLKTQQMAYSNAQTSLNQ
                     IMGVGLEQAYEFPTDLKSSFTPQLEEIRKQAMEQNAGLLKARSNLSLNSLRLREVKAE
                     RMPILKLNTGYNFNRSSSSAGFALFNQSNGLNGGLTLSWNLFNGGQLKTRMTQAQFAF
                     ENDAIKIRQAETQLNASIQIAIRQWQQASELLKLEDENFISAKENLSLALERVRLGNA
                     GILVVKEAQRSYEEAINRQSAAALSAKQAEIDLMQLSGKLIQ"
     gene            complement(8877..9356)
                     /locus_tag="PK281_05925"
     CDS             complement(8877..9356)
                     /locus_tag="PK281_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48917.1"
                     /translation="MLSTKYLLYLVSFFSISILCSCEKFEQDEGIQWHKPEKRIQQYS
                     WSISKYLVDGIDSTDKKKEFLHNATFRFYINDKNSYIASYRTDQDEMPFAFWGINKKS
                     PDAFFIVIWNNYNAIKLDSTFDVNLIRLGGYYKIQKLSNKTLKLKSQENFVIDFIGN"
BASE COUNT         2717 a         1956 c         1999 g         2800 t
ORIGIN      
        1 aacacaatca tcttctcctt ggtaaacgtt ccttcggctt ggccttttag gaagacttcg
       61 aatgtgtagg gtttgaaaag tgcgtttttg acttcaatca cataagtgcg ttcaccttct
      121 aaagtgataa agtaatcacc attttcgtcg ctgcttacgc ttaccacttt gccgttttct
      181 ttatcagtaa tgctgatttc ggtttcgatg gtttgtccgg cgtcggagtc gattacttta
      241 ccgtttaaga ttgaaagtgg cggtcctttg ggaacttgaa cgggagcttt ggaggagtcg
      301 gttttgactt gagtttgaag agcagagaaa tcgatttccc agatatctaa atcgcctgtt
      361 ccaccttcgc ggttggagct aaaccaagct agacgaccat cggtgctgag ggcaaaaaag
      421 cgttcgtcac ctgtggtgtt gatcggatat cctaagtttt cgggaggtgt acatttaccg
      481 ttttcgacgg tggttttgaa aatatcgtaa ccgcccaaac ttttagtaga gttggatgcg
      541 aaatacaaag ttttgccatc gggatgcatg aataaaccaa tttggtcgaa ttcatcattc
      601 accactgaac ctaaattaac agcatcgccc cattcggttt tgctcttctt tttagccatg
      661 tagatatcgc ccattccgaa accaccacga gggcgttcgc ttacgaaata caaggttttg
      721 ccatcgggag aaatacaggc agaactctcg aaataactgg tgtttacatc accttcaact
      781 ggttttgtag cactccattt acctgcacgg gattttttcg aaacaaacaa ttctcctgaa
      841 ccacgataac ccatgttgcg atacacaaaa atctgactgc catcaggcgt caaagtcata
      901 ttagcatcgt gagtttcggt attgagcgag ccgggaatat tttccgcttc caaccatttt
      961 cctgtgatgg aatctcgaac agaaatatat atgtcttcgt aaaatttgcc gtcttctgga
     1021 tcagttagtt ttccggtaga ttcgggacga cgagaagtga aaatcatcgt tttcccatca
     1081 gccgaaacac ttggattgta ttcaggatat tccgaattga tagcttctcc aatgttgcga
     1141 atttgcacat tcacgggttt cgacatcaat tctaccgcac gacggcactg atctaacaaa
     1201 gcggtagctt cttcggtatc atcgttcttc ttaggtgatt tctcaatgaa atgttggtat
     1261 tcaacaatag cttttgacag ctgtcccatt tgctggtagg tttggcccaa gaagtaatat
     1321 ttctctggat cattcaccgc atctaaccgg atggaacgct ctaaacgctc ttttgcatcc
     1381 tcgtaatttt ttaagtgaaa attgcattct ccactcatga aatagagctc ggcagtggtg
     1441 ctgtcggtct tctccatatc acgtaacgaa cgccaagcag tggcataatc gccgcccatc
     1501 atggtgtttt ttatccgaag taattgcagc ttgttggctc ctttatcggc atataaatta
     1561 ccttgtgtta caggcagaat tagcaacaat agcaagagat tacgcagcat ttgaatcttc
     1621 attcctgagt gttttttctt ttctctaaag taggaaatca ttggctttcc agtagagtaa
     1681 ttgtactaag tgctgataaa aatgtttcta agcctgctcg cttaggggaa tctagcgtgt
     1741 aagaaattcg ttcgctgagg taattcaata ccggataatg tgccggataa ttcgcctcca
     1801 ccgattttgc tgctaattca atattcgaaa ttcccaatag caacgcgtct tccacctttt
     1861 gccacttttc atccgcaatc ggattaatcg aagcccaaca ggcaaacaca aatggttgaa
     1921 gcttccaatt catccaagct tcgctcaaat catatacata cttgaatcga tgggcataat
     1981 caaatacacg atcgccaata agcacaccgg cacgttttcc tcccaaagtg ctgagaaaat
     2041 caggcttcgc cgaagtaaaa acagggttga ttttccagta atctcgcgcc aacagccgac
     2101 atagattcac cgaagtacgg gattgaaaat cgagcacgat ttcttctatt tcctccaatg
     2161 gcacatcact catcaacacc acagaatcta ctttcccgac cgcgccaata caggtgtttc
     2221 ccaagattga atactgaggt agagaaagta aagttgctac aggaactaat cctacatcca
     2281 ccattcctga ccgtaattta tcggcacaaa ctgctggaat atccaaacta atctggattg
     2341 aatcggattc taataagccg gaacgctcca atccatatag aaaaggcgcg gtgttgaggt
     2401 aggaaacgac agagagtcga agcatgggtg caatatcgtt gaattatcac gcgggtcgat
     2461 tttgtgcgtg attttttgac aaggctacaa caattcgtac gtactttagt ttactttgca
     2521 aaccatttct attggcaact aaatttccat ggctcaacca cttttatttg agacacttcc
     2581 gttagggcgt cacttagcag tccttacacg attgtattat ggtgcgctca cacttgaact
     2641 ttccgacctt gggattgatc gttactactc cgttttgatg tacatcgatc gttccgaaca
     2701 tccagttacg caacaaatgg tcggtgaatt tttgctcatg gataaaactt ccatggttcg
     2761 agtgatcgat tttttggtcg aaaaaggata cgtgaaacgc gtgaccaatc ccaacgaccg
     2821 tcgctcttac atcatcgaac tcaccgaaaa aggacacgaa atcctcccca agattataca
     2881 agccgttgcg cagataaatg atcaagttat ggaaggattg tcggaagcag aacgtgcaca
     2941 atttcatcat acactctgtc atatttctac tcgtctcaag aaaatgccag ctaaagacat
     3001 acaaattgac tttcaaccca acccgaacaa ttttcctcaa taagctatga aacgtaccct
     3061 aatcctgatt ctaggcatag ccttgttaat cctgatcaaa atcttcttcc tcacgaaaaa
     3121 ggaagatccc agtaaatcgg gcccccctgg taaaggtcaa cctacaccgg tgaccgtgta
     3181 tattgttgat tcttcccgga ttgataatca aatcacttct gttggaacac tccgttccga
     3241 agaagaagta gatcttcaac ccgaagtttc tggaaagatt accagcattc attttcgcga
     3301 gggcgatcgc gtgagtaaag gacagctttt actccaactc aacaatgccg atttgttagc
     3361 tcaattgggt aaactcaacg tgcaacgcaa agttcaggaa gagaaagcgg gacgtttgaa
     3421 aagcttgctt caaatgcagg gtagcagtca ggaagagtac gaccaagcac aaaacgtggt
     3481 tgccagcacc gatgccgaca tcaagtatat acaggctcaa ctagctaaaa ccgaagttcg
     3541 cgctccattt tcgggtgtga ttggtttacg ttcggtgagt gaaggcgctt ttgtaactgc
     3601 caatacacgc attgctcgtt tgcaacaaac cgatcaattg aaactcgatt tcaatgttcc
     3661 agaacgttac atttctttgc ttgaattgcc cttgaaagtc gcctttggag tacaaggtcg
     3721 ttcggataaa tttgaagcaa acgtatatgc tatcgaaccg agtgttagca atactacacg
     3781 tacggttcag cttcgtgctc gggttagtaa taatgctcaa caattgttac ccggacaatt
     3841 tgctcaagtg ctcctgccca ttaaaaatga tcattcctcc attttaattc ctacccaagc
     3901 catcatcccc attttaaagg gacaaaaagt gtttgtctgc aagcaaggga aagcggccga
     3961 agtgaaggta gaaacgggtt atcggaacga tgcgatgatt gaagtacttt cgggtttaag
     4021 tgtgggcgat agtgtgatta ccacaggaat tatgtcgctt aaggctgatg caccgctgaa
     4081 aattatacct gctaaatcac aaaagaagga ggttcgttca tgagtagctt atcgaccata
     4141 agcatccgaa ggccagtatt ggctatcgtg atgaacctca ttattatgct gtttggctgg
     4201 attggatata cctatttggg tgtacgcgaa tatccatcca tcgatccgcc agtaattacc
     4261 gttcgtacgg gttatgcagg tgcgaatgct gatgtgattg agtcgcagat taccgagcca
     4321 ctcgaaaaag ccttgaatgg tatcgaagga attagaacca tctcttccgc cagtaatcag
     4381 ggaagtagcg taattacagt ggagtttaat ctcggttcta atctcgaagc tgctgctaat
     4441 gatgtgcgcg acaaagtttc tcaagctgta cgcgatttac ctaaagatat tgatggatta
     4501 cccaccgttt ctaaggcaga tgcgaattcg gatgcgattt taagtctgac cattgaaagc
     4561 aataccaaaa cccaactcga agtaagcgac tatgccgaaa acgtgattgg ggaacgcctg
     4621 cagacgattc cgggtgtgag ttctttacaa atttggggac agaagaaata tgctatgcga
     4681 ttgtggatgg acccttcgcg tatggctgca ttgggcatta ctcccatgga tgtgcaaaat
     4741 gctttaatac agcaaaacgt agaacttcca ggtgggaaaa ttgagggaaa tgctaccgag
     4801 cttagcgtga agaccgttgg tcgtttgcgt agtacggaag aattcaacaa catgattatc
     4861 cggaacgaaa gtggcgtaaa tgttcgcttt tccgatattg gtgaagcgcg tttaggaccc
     4921 gaaaacgagg aaactatttt acgtcagagt ggaacgccca tggttgcttg tgctttggtt
     4981 cctcagccag gtgcgaatta cctcgaaatt gccaaagagt tttacaaacg ggtggatgag
     5041 attaaaaaag atctaccaga tggtttcaaa ctcggcatcg cgctagataa tactcgattt
     5101 gtttctcaaa gtgtggatga agtagtggaa acgctcctca ttgccatttt gctggtaatc
     5161 atcatcattt acttgttctt ccgcgattgg ttgattgcta ttcgtccgct tatcgatatt
     5221 cccgtttcgc tcttaggtgc ctttttcatc atgtatgcga tgggttattc gatcaatgta
     5281 ctcacgctct tggcaattgt cttggcgaca ggtttagtgg tggatgatgg tattgtagta
     5341 accgagaata tctataagaa aattgaaggt ggcatgaatc cgattgaagc tgccgagaaa
     5401 ggttcgaacg agattttctt tgcggtgatt tcaacttctg taacactcat cgcggtgttt
     5461 atgccggtga tttttctgga aggatttgta ggtcgattgt tccgagaatt tggagttgtg
     5521 ctagcgggtg ctgtagctat ttcggcattt gtgtctttaa cgctaacacc catgctgaat
     5581 gcgcgtttgg tgcgaaaaga tccgaccaag aaaagtaagt tttacgtagc aactgaacca
     5641 ttctttcgca gcatggaaga aggttattcc aacatgctgg gcagtttcat gaaaaaacgc
     5701 aaactcgctt ggttgattct tctcggtgca gtagccatga ttttcggaat atttccgctg
     5761 ctgcaaaagg aattatctcc tttagaagac cgaagttgga tgcgtttaat ggttagtgct
     5821 cccgaaggtg cttcgtatga atataccgat gattttgtgt tgaaaatgtc ggatttcttg
     5881 agtgattcgg ttcccgaaaa gaatgtgata ctgacggtta cagctcccgg ttttgcgggt
     5941 tcgggtgccg tgaattcagg atttattcgc ttgactttat caccacctga cacgcgtaaa
     6001 cgcacgcagc aggatattgc caattacgtg atgaaaaaag ccaagggttt tcctcaggct
     6061 aaggtatttg ccattcagca acaaacgatt tcaaccggtg gatttggtgg cttgcccgtt
     6121 caatttgtgc tacaagctcc caattttgaa cgtttaaaag aagtgcttcc taaattcttg
     6181 gaagaagcca ataagaatcc cattttccaa ggtgtggatg tcaacctgaa gttcaacaaa
     6241 ccggaactta acgttagcat caaccgcgac aaagccgaaa gcatgggtgt atcggttcag
     6301 gcgattgctc aaaccttgca attatccttg agcggttcgc ggatggccta ttttaccatg
     6361 aatggaaagc agtatcaggt tattggtcaa gttgatcggg taaaccgaga tgagccaatc
     6421 gacttgaaat cactttcggt gcggacgaac acgggtgaat tagtgcagtt ggataaccta
     6481 gttgacgtgg ccgaagtgag cagtccgccg caattgtacc attacaaccg ttattcttca
     6541 gcaacagttt ccgctggtct agcgcccgga aaaaccattg gtgatggaat caaagccatg
     6601 aaggatattt cggcaaaact gttagatgat tcgttctcta cttcattaac aggagcttcg
     6661 cgcgactttg aagaaagttc ttccaatatt ctatttgcct ttttactcgc gctcttgctt
     6721 atttacctcg tccttgctgc tcaattcgaa agtttccgcg atccactggt gattatgatt
     6781 acggttcctt tggcacttgc tggtgcatta ctttctcttt ggtatttcaa tcaaacactc
     6841 aacatcttca gcgaaattgg gatgattatg ctcattggtt tggtcaccaa aaacggaatt
     6901 ctaatcgtcg aatttgccaa ccaacttcag gaacggggag aagataaagc cagcgctatc
     6961 ttaaacgctg catcggctcg tttacgtccc attttgatga ccactctagc tactgcactt
     7021 ggtgctttac ctattgcatt ggctttagga gccggtgctg taagtcgcat gggaatggga
     7081 atcgtcatta ttggcggatt gctcttttcg cttgccttaa ccttatttgt cattccagct
     7141 atgtattcat tcatcgctaa aacccgcgtg ccacatgaaa aaaactaatt ggattgcagt
     7201 tttaatcttg tcctgcacca ccgtgttaaa cgcgcaggaa gtgcttagcc ctgaaaaagc
     7261 cgttgagctg gcacttaaga acaactattc gatattaatc gctcgaaatg agcaactttc
     7321 ttctgatgct caagttacac gcggaaatgc tggaatgctt cctcgagttg atgtggctgc
     7381 cggagcatct gctcaaagca caaacatcaa ccaacgtttt tccaacggac taaccgtagc
     7441 tacaaatggt gttagtaata gcgccagcaa tgcttctgct gtgttaagct ggactttgtt
     7501 tgatggtggt aaaatgttcc tcacctggga tcgactaaaa gaacagaaat ccctaaatca
     7561 attgcaattg cgcgaacaag tcgagcaaaa tattgcagct gtgttggatg cgtatttttt
     7621 ggtcattcga cttaagcaag aagtccgttc tcgacaattt gcgctggatg taaccgaaga
     7681 gcaattcaat ctcacccgta aaaaggtaga attaggctca tcatcccgac aagaattatt
     7741 gcaagtaacg gtcgatcgaa acactgcttt gtcgcaactg aaaacgcaac aaatggcata
     7801 cagtaacgcc caaaccagtt tgaatcaaat catgggtgtt gggttggaac aagcgtatga
     7861 atttccgaca gatttgaaaa gcagttttac tcctcaattg gaggaaattc gtaagcaagc
     7921 aatggaacaa aatgccggac tgctcaaagc gagaagcaat ctaagtttga acagtttgcg
     7981 cttacgggaa gtgaaagccg aacgtatgcc gattctaaaa ttaaacaccg gttataactt
     8041 caaccgctcg agtagttcgg ccggttttgc cttattcaac caaagtaacg gactaaacgg
     8101 tggattgaca ttaagttgga atctattcaa cggcggacaa ttaaaaaccc gcatgactca
     8161 agctcaattt gcatttgaga acgatgccat caaaattcgt caagccgaaa cacagttgaa
     8221 tgcctccata caaattgcta ttcgtcaatg gcagcaagct tcggaattat taaaactgga
     8281 ggatgagaat ttcatttccg ccaaagagaa cttaagtctg gcattggaac gtgttcgttt
     8341 gggtaatgct ggaattttgg ttgttaaaga agctcagcgc agttacgaag aagcgatcaa
     8401 ccgtcaatca gcagcagctt tgtcggctaa acaagcagag attgatttaa tgcaacttag
     8461 tgggaaactt attcaataga ttaattacga attttcaatt acgaactacg aattaagtac
     8521 aatgtattat gtacaacgta ttaagtacaa cgtattacgt attacgaaaa tgaattacga
     8581 attaagtaca acgtatttcg tattgcgaaa aagaattacg aattaaatac aaggatttga
     8641 gtgaattgat taaagaattt gagtgaacag atttctaatg tgaatatcaa tatgcttgaa
     8701 cattatattc aaagtcaggt aaactgcaac ccatgcaatc tccaaccttt aggaatctat
     8761 ttcttagttc ggttcgatac ctccaatatt ttttgattgt ttaatatatc attttgagac
     8821 aataaattcg cacttaaaac actagaaata ttattgtttt cattttattt tattttttaa
     8881 tttccaataa aatcaattac aaaattttct tgacttttta attttaaagt tttatttgaa
     8941 agtttttgga ttttataata cccacctaat cgaattaaat taacatcgaa agttgaatct
     9001 aattttattg cattataatt attccatata actataaaaa aagcatcagg tgatttttta
     9061 tttatacccc aaaaggcaaa gggcatttca tcttgatctg ttctatatga agctatgtaa
     9121 gaattcttat cattaatata aaatctaaaa gtggcattat gtaaaaactc ttttttctta
     9181 tctgttgaat caattccatc aactaaatat tttgaaattg accatgaata ttgctgtatt
     9241 ctcttctcag gcttatgcca ttgaattcct tcatcttgtt caaatttttc gcacgaacac
     9301 aaaattgaaa ttgagaaaaa tgatactaaa tatagtaggt attttgtgct taacataagt
     9361 aaaattgaaa agtgttaata cttaataatt tgtattgatt gaattaattt ttgggaccac
     9421 ccatgaattc aatgtatgta ttgggatctt tttgaagttt gaattgatag tc
//
LOCUS       DAOOQW010000075         9431 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_9032_length_9431_cov_5.294262, whole genome shotgun sequence.
ACCESSION   DAOOQW010000075 DAOOQW010000000
VERSION     DAOOQW010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 9431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9431
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9032_length_9431_cov_5.294262"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="PK281_05930"
     CDS             complement(<1..315)
                     /locus_tag="PK281_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48918.1"
                     /translation="MSKRSGNFPFWMLTFAFICLVLVSQLIQDGMFMDGMIYVTVSKN
                     LAEGLGTFWDPYFSQTYLTSYHEQPPLYFGLLALFFKILGNSMYVERLFCLCCFSVTA
                     FYI"
     gene            complement(352..720)
                     /locus_tag="PK281_05935"
     CDS             complement(352..720)
                     /locus_tag="PK281_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48919.1"
                     /translation="MEEYNILDQPEQNANTQERPVIITVICILGFLGAAITIPLIFSE
                     LAKSVATWYPFYLAFAATIGLICMLGLWKMKKWAALLYTAFVAMNQLLLYTLGAWTLF
                     SFIVPAVVVAIALSQLNKMS"
     gene            complement(758..3358)
                     /locus_tag="PK281_05940"
     CDS             complement(758..3358)
                     /locus_tag="PK281_05940"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOY48920.1"
                     /translation="MNFRYVLILLACVFGIQANSQSTISPFLVQRIEQNYLAVQRIQI
                     YMRDRVDTRQMSIDFQQEQRSQDERVRTLNQSLENRAEQSQRPVIDFIQSYLGAEAAQ
                     QAIVRQFHIVNMMVVDATPDLIYQLAALPQIEHIESNNRFSITPVPIVEKHPAQSRSV
                     GGHEAGLEAIHAPFMWNLGYTGLGRKLFTCDTGVWPVHPAIRRQYRGNFYPQAMCWKG
                     FDSETPADKPDAHGTHVTGTILGLDTATADTIGIAFNATYMASDPIVEDPADIKPVTV
                     IFEAFEFAINPDGDVATSYDVPDVICNSWGVGDSIFEGLCTAPFMIDLFTSLDAAGIA
                     VEFSAGNEGPGAGTIGLPQYVTFDSLSIFTVGALNANTSSLPIASFSSRGPTSCDVID
                     AWKIKPEVSAPGVDVRSSVQWDQYAEYSGTSMAGPHVAGAVLLLKEAFPYLTGRDLLN
                     ALYQTATDLGTLGEDNTYGRGMINLEAAYNFLASTNTPVLPNTSGFDIAIVDIVGEGF
                     ACSENYQPTIILKNIGTEIISGGMINWTIDGLGGGTSMWNGTLMPGAQTYITLTPATL
                     TTGNHEIIATFIPTGNFIERDLDNNSRVKHVYVQPNVELPYAESFENNDLSANNWLKI
                     NYDNSKTWDTTHTAGLTDSRYSARMNFFNYPTRKQIDDLITPVVNLPASLDSLFLRFD
                     LAYRLRNTSVSDSLDISISSNCGESWTSIFHRGGQEIETLDTVWTNFRPFSASHWDYY
                     KLDLLPYASGNSIIVRFRGINGAGTNLYLDNIGIYSASDPTGIEDLDMQASVFPNPAT
                     SQVCVQVNKSNTSVAVQLIDLQGRLIQTSTIVKGTNQTFLNLNQLSKGVYLIRLQSGE
                     HVQVKRLVIQ"
     gene            complement(3399..3884)
                     /locus_tag="PK281_05945"
     CDS             complement(3399..3884)
                     /locus_tag="PK281_05945"
                     /inference="COORDINATES: protein motif:HMM:NF013231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOY48921.1"
                     /translation="MSKKVSEVSVKASSARHPFGDPEQNAGFVMWQVSMLWQRKLKTG
                     LDTIGITHAQFLLLAALQFIGTQKNIVSQQDLAKHCRIDKMMTSKILRTLQKKGLLSR
                     KKNKMDTRSKTLALSEEGEDLLAKAFKIIDRVDGDFVVPLGLNSMSFTDDLRTLLKQN
                     S"
     gene            complement(3998..4861)
                     /gene="prmC"
                     /locus_tag="PK281_05950"
     CDS             complement(3998..4861)
                     /gene="prmC"
                     /locus_tag="PK281_05950"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR03534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOY48922.1"
                     /translation="MKIPGNSIAQIKQYFESQLKDIYTANEILALFRMAAAEINGWSM
                     IQLHLEPNTRVNESDLLLYSRFVKRLRNQEPVQYILGSAWFCGLEIGVKPGVLIPRPE
                     TEELVNGFLEQITIDAPRILDACTGSGCIALAVKNYLPKAEVFACDISKVALKIAQEN
                     SDKLALPIHLFEADILGAIPENIAPFDFILSNPPYIPHSERASMSAHVHEHEPEMALF
                     VPDSDAMLFYRNLARWGNSILKPGGMLLAESHSAFTQEVKTCWEEAGFTQIEIHTDLQ
                     GLPRWLHAVKI"
     gene            4963..6036
                     /gene="ribD"
                     /locus_tag="PK281_05955"
     CDS             4963..6036
                     /gene="ribD"
                     /locus_tag="PK281_05955"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HOY48923.1"
                     /translation="MQRALELAAQGLGKVAPNPLVGCVIVNQDTIIGEGFHQRFGGMH
                     AEVNAINSVADKSLLSDSTLYVTLEPCNHQGLTPPCCDIILEHKIPRVVIATLDSNPL
                     VSGKGVERLRQAGVNVQVGLLEEKARWQNRRFFTFHERYRPYIILKWAETKDGFMDIL
                     RENGETGSFAISGKDAKILVHKWRTEEASILVGSRTVFIDNPQMTARHWPGKDPVRIV
                     IDANLKVPRTHKIFEDRGRTIVFNRLETRPIFNIEYIQLNFAEEILPRIMDYLRYEKL
                     TSVIVEGGAATLTRFLELGLWDEIRRFRAPMNLDKGLKSPVLDLKPYESVTIGEDQLD
                     FFYQQIPPAGFKSISDNPFEANS"
     gene            complement(6001..7686)
                     /locus_tag="PK281_05960"
     CDS             complement(6001..7686)
                     /locus_tag="PK281_05960"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY48924.1"
                     /translation="MKNWGIVTGLFCLCTSIHAQQLTWGHAMGGASADNLSRIAMDAD
                     HNVFAAGTYTAPTQSSLGALPAYGFQDVVVNKYSPTGTLLWQIGIGGANTDLVSAIAC
                     NSEGDIWISGRFQGSIDLDPSDSTEIIGSDPSGSLDGYLAKYDGETGQLLDFRNITSG
                     GTIDIRSIRIDVFDDIFIAGQFTNTVDFDFGGGSYSKTSNVFSGDCFVGRYYSDLQLV
                     WMNVIGSQTPAIDYIGDMCLSADGYVYCTGLLGGTADINPGVGVTNLTAAVDAFLVKY
                     SQNDGTLSWGFLLGGSSLDLGTSVMMTEDNQIVIAGSMNSTSMDVDPGVAQTTLTKTG
                     SNASPFLARYTNSAVLVSAVLMQGSQTLTATINRITQGQSNSILITGSYKGDVDIELG
                     DSSFNISSGDSANSYILRCASDWSLIQYFQQGGNGDQTGYDLIGEGTNVFLVGQMSNT
                     LHPEFPANTNLIPRNGNGLDGFVLKYNFVPDALSLNEQQNQDFHVFPNPTSGLINIGT
                     TNIQSCSIIDVTGRIQKLEVLNNQVDIETYPSGIYTLILENNSGISFKRIVRN"
     gene            complement(7832..8995)
                     /locus_tag="PK281_05965"
     CDS             complement(7832..8995)
                     /locus_tag="PK281_05965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CofH family radical SAM protein"
                     /protein_id="HOY48925.1"
                     /translation="MLNKFYLAPVMDSMNVQALLEKALRMEWLTIEEGVFLFHQASTP
                     ELMFVGNELRKRQKPDGKVTWMIDRNVNTTNVCIANCKFCNFYRIPGHAESYITTIDQ
                     YRVKIEEMRRLGGDQLLLQGGHHPELGIDFYEQLFRDLKLHFPDIKLHALGPPEIAHI
                     AKLSQLTHTEVLVRLKAAGLDSLPGAGAEILNDRVRRMISKGKCSGKEWLDVMRAAHQ
                     VDITTSATMMFGHIETVEERFEHLAWIRQVQSEKPANAKGFLAFIPWPFMDDGTLLRR
                     VKNVRNQVTADEYIRMIALSRMMLPNVINIQASWLTVGENTAQLCLHAGANDFGSIMI
                     EENVVSAAGAPHRFTSAGIQEAIRLAGFEPQLRSQQYDYREIPAEIEQQVIGY"
     gene            9177..>9431
                     /locus_tag="PK281_05970"
     CDS             9177..>9431
                     /locus_tag="PK281_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48926.1"
                     /translation="MSTISISRITKAGKGDRVLVASKTEDLKNQGFSKEEYSYIQAQS
                     KLQRKSIAVNHFGHFSFVVLIDTKKTEFLTLEAQRVAGNAL"
BASE COUNT         3007 a         1900 c         1749 g         2775 t
ORIGIN      
        1 aatataaaat gctgttacag aaaagcagca taaacaaaac aaacgctcaa catacatgct
       61 gtttcccaat attttgaaaa acagtgctaa taagccaaaa tacaaaggtg gctgttcgtg
      121 ataggaagtt aaatatgttt ggctaaaata aggatcccaa aaagtgccta atccttcggc
      181 gagattttta cttacagtaa cataaatcat cccgtccata aacatcccat cttgaattaa
      241 ttgggaaacg agtactagac agatgaaagc aaatgttagc atccagaacg ggaaattgcc
      301 cgaacgtttt gacatgccta aaaatttaat ctccattaaa ttgatagttg attaactcat
      361 tttattgagt tgactaaggg caattgcaac aactactgca ggaacaataa aagaaaataa
      421 tgtccatgcc cctagcgtat aaagtaataa ttgattcatg gcaacaaatg cggtgtataa
      481 caatgcagcc cattttttca ttttccataa acccagcata catatcaatc caattgtagc
      541 tgcaaatgct aaataaaatg gataccaagt agctacgctt ttggctaatt cggagaaaat
      601 taaaggtatt gtaatagcag caccaagaaa tcctaaaatg caaattacag ttatgataac
      661 tggacgttct tgagtattag cattttgttc gggctgatcc aggatattat attcttccat
      721 gtttaagcta ttatgatgaa tgattaaata gcaagtgtta ttgaataact aaacgcttaa
      781 cctgcacatg ttcgcccgat tgcagtcgaa ttaaataaac acctttactc aattgattta
      841 agtttaaaaa tgtttgattc gttcctttta caattgttga agtttgaatt aatcgacctt
      901 gtaaatcaat caactgaaca gcaaccgaag tgtttgattt atttacctga acacaaactt
      961 gtgatgtagc tggatttggg aaaacactgg cttgcatatc caaatcttcg attcccgtag
     1021 ggtcagatgc agaataaata ccaatattat cgagatataa atttgttcca gcaccattaa
     1081 ttcctctaaa acgaactatg atggaatttc cagaagcata tggcaataaa tctaatttat
     1141 aataatccca atgggaagca ctgaatggtc ggaaattagt ccaaacggta tccaatgttt
     1201 ctatttcctg tccgccacga tgaaatatag atgtccagct ttctccacaa tttgaagaaa
     1261 tagaaatatc taaagaatca ctcaccgatg tatttcttaa gcgataagct agatcgaaac
     1321 gcaaaaacaa agaatcaagg ctagctggta gatttacaac aggtgtaatt aaatcatcaa
     1381 tttgtttgcg agttggatag ttgaagaagt tcatacgtgc agaatagcgg ctatcggtta
     1441 atcctgctgt atgtgttgta tcccaagtct tagaattatc gtaatttatt ttcagccaat
     1501 tatttgccga caaatcatta ttctcaaaac tctctgcata gggaagttct acattcggtt
     1561 gaacatatac gtgtttaaca cgactgttat tatctaaatc gcgctcgata aaattacctg
     1621 ttggaataaa cgtagcaatg atttcgtgat taccggttgt taatgttgct ggtgttaaag
     1681 tgatataggt ttgagctcct ggcatcaaag taccattcca catggatgtt ccgccaccta
     1741 aaccatcaat cgtccaattt atcatcccgc cactaataat ctcagttcct atatttttca
     1801 aaattattgt tggctgataa ttctctgaac acgcaaaacc ttcgcctaca atatcaacaa
     1861 ttgcaatatc aaatcctgaa gtattaggta atacaggcgt gtttgtacta gctaagaaat
     1921 tataggcagc ttccaaattg atcattcctc gaccataagt gttatcttca cctaatgttc
     1981 ctaagtcggt tgctgtttgg taaagtgcat tcaacaaatc tcttcctgtt agatatggaa
     2041 atgcttcttt caataataat acagcacctg ccacgtgtgg accagccatt gaagttcctg
     2101 aatactctgc atattgatcc cattgaacgc tcgaacgaac atctacaccc ggagccgaaa
     2161 cttcaggttt gattttccaa gcatcaataa catcacatga agttggtccg cgactagaaa
     2221 aactagcaat tggaagcgaa ctcgtatttg catttaaagc cccaacagta aatatggata
     2281 gactatcaaa cgttacatat tgtggcaatc ctatcgttcc agcacctggt ccttcatttc
     2341 cggcagaaaa ttctactgca atgcctgctg cgtcgagtga tgtaaataaa tcaatcatga
     2401 aaggagctgt acataatcct tcaaaaatag aatcaccaac accccaagaa ttacaaatca
     2461 catcgggtac atcatagcta gttgctacat caccgtcagg attaatagca aattcaaatg
     2521 cttcaaaaat gaccgttact ggtttgatgt ctgctggatc ttctacgatt ggatcagaag
     2581 ccatgtatgt agcattgaag gcaattccga ttgtatcggc tgtagcagta tctaatccta
     2641 atatagttcc agtaacgtgc gttccgtgtg catcaggttt atcggctggt gtttcggaat
     2701 cgaagccttt ccaacacata gcctgaggat agaaattacc ccgatattga cgacgaatgg
     2761 caggatgcac aggccaaact ccggtatcac aggtaaatag ttttcgacct aatcccgtat
     2821 aacccaaatt ccacataaat ggagcatgta tcgcttctaa accagcttcg tgtccgccta
     2881 ctgaacgcga ttgtgctgga tgtttttcta caattggaac tggtgtgatg gaaaaacgat
     2941 tattcgattc gatgtgctca atttgaggaa gagctgctaa ttgatagatt aaatcgggag
     3001 ttgcatcaac aaccatcatg tttacaatgt ggaactgacg gacgattgct tgctgtgcag
     3061 cctcagcacc caaatacgat tgaataaaat ctataacggg tcgttgtgat tgttctgctc
     3121 gattttctag ggattgattt aatgtccgaa cacgctcatc ctgagaacgt tgctcttgct
     3181 gaaaatcaat gctcatttgt cgcgtatcaa cccgatcacg catgtaaatt tgaatacgtt
     3241 gaacagctag gtaattttgc tcaatccttt gaacaaggaa aggactaata gtagattggg
     3301 aattagcttg aatgccaaat acacaagcca gaagaatcaa aacgtaacga aaattcatgc
     3361 ggataacttt ccgccaagtt aaataaaagc aatcaagctt aactattctg cttcaaaagt
     3421 gtgcgtaaat cgtcggtaaa cgacatggag ttcaatccta gtggcactac gaaatcacca
     3481 tcaacacgat caataatttt aaaagctttt gctaataaat cttctccttc ttcacttaaa
     3541 gccaaggttt tagaacgagt atccatttta ttcttcttgc gacttaacag accttttttc
     3601 tgtaacgtgc gaaggatttt agatgtcatc atcttgtcga tacgacaatg tttggcaaga
     3661 tcttgttgtg aaactatatt tttttgagtt ccaataaatt gaagtgcagc cagtaataga
     3721 aactgtgcat gagtaatacc aatagtgtct aaaccagttt tgagtttacg ttgccaaagc
     3781 atcgaaactt gccacattac aaaaccagcg ttctgctccg gatcgccaaa agggtgacgt
     3841 gcggaagagg ctttcacaga aacctcagaa actttcttag acataccaat tagtattaaa
     3901 gttgcaagta taagcaaatt cgatttcctg caagtactta ggcggaacaa agaatgtaat
     3961 attcgctact taactagaaa acagcggatt tcaattctta aattttgacc gcatgtagcc
     4021 aacgaggtag tccttgcaaa tcggtatgaa tttcaatttg tgtaaatcca gcttcttccc
     4081 aacatgtctt tacttcttgc gtgaaagcag aatgactttc tgccaataac atccctccag
     4141 gttttaaaat ggaatttccc caacgggcaa gattgcgata gaaaagcatt gcatccgaat
     4201 caggaacgaa aagcgccatt tcgggttcgt gttcatgaac gtgtgccgac atgctggctc
     4261 tttctgaatg aggaatatag ggtggattgg ataggatgaa atcgaatgga gctatgtttt
     4321 cgggaatagc gcctaatata tcagcttcaa aaagatgaat gggaagtgct agtttatcgg
     4381 aattttcttg agctattttc aaagctactt tagaaatatc acaggcaaag acttctgctt
     4441 taggaaggta attttttact gcaagggcaa tgcaaccact accagtacac gcatcaagaa
     4501 tacgaggagc atcaattgtt atttgctcta gaaaaccatt gactaattct tccgtttcgg
     4561 gacgaggaat taaaacacca ggcttcactc ctatttctaa tccacaaaac caagcagaac
     4621 ctaagatata ctgaacaggt tcttgatttc gtaatcgttt cacgaaacga gaataaagaa
     4681 gcaaatcact ttcattcaca cgggtgttag gttcaagatg tagttgaatc attgaccagc
     4741 cgttgatctc tgctgcagcc attcgaaaga gagccagaat ctcgttggct gtgtatatgt
     4801 ctttcagttg cgactcgaaa tactgtttga tttgggcaat gctatttccc ggaattttca
     4861 tgaaaaaagt taataaagta ggttgacaaa ggacagaaag atgtgggaga tacacactat
     4921 tgaaatcatg tttttagcta ttttggcagc atggaaatgt atatgcagcg tgctctcgag
     4981 ctagcagctc agggcttagg taaagtagca cccaatccgc tagttggttg tgtgattgta
     5041 aatcaagata ctatcatagg tgaaggattt caccaacgtt ttgggggaat gcatgccgaa
     5101 gtaaatgcca taaattcggt tgctgataaa agtttattat ccgattctac attatatgtg
     5161 acgctagaac cttgtaatca tcagggatta acacctccat gttgtgatat aattttagaa
     5221 cataaaatcc cacgtgttgt aattgccact ttggattcaa atccgctcgt atcaggcaaa
     5281 ggtgtagaac gcttacgtca ggctggagtg aatgtacaag ttggattact ggaagaaaaa
     5341 gctcgttggc agaatcgtcg ttttttcacg tttcacgaac gatatcgccc ttatatcata
     5401 cttaaatggg ctgaaactaa agatggtttc atggatattc ttcgtgagaa tggagaaact
     5461 ggaagctttg ctattagtgg gaaagacgcg aaaatattag ttcataaatg gcgtaccgaa
     5521 gaagcttcta ttttagttgg atcacgaaca gtttttatcg ataatcctca aatgacagct
     5581 cgccattggc ccggaaaaga tccggttcgt attgtgattg atgccaattt gaaagttcca
     5641 cgcacgcata aaatattcga agatcgtggt cgtaccattg ttttcaaccg cctcgaaacg
     5701 cgtccaattt ttaacattga gtatatccaa ttgaattttg ccgaggaaat tcttccgcga
     5761 atcatggatt atttgcggta cgagaaactc acatcagtca ttgtagaagg tggtgcagca
     5821 acgctaaccc gctttttaga attgggttta tgggatgaaa tacgtcgatt cagagcaccg
     5881 atgaatcttg acaaaggttt gaaatctcct gtattggatt tgaaacctta tgaatccgtt
     5941 acgattggag aagatcagct ggattttttc tatcagcaaa ttcctccagc tggatttaaa
     6001 tcaatttctg acaatccgtt tgaagctaat tcctgaatta ttttccaaga ttagcgtata
     6061 aattcccgaa ggataggttt caatatcaac ctgattattc aaaacttcca acttttgaat
     6121 tcttcctgtt acatcaataa tcgaacagga ttgaatgttg gtagttccaa tgtttattaa
     6181 accagatgtt ggattaggaa acacgtgaaa gtcttgattt tgttgttcgt tcagcgacaa
     6241 agcatctgga acaaaattat acttcaatac aaaaccatct aagccatttc catttcgagg
     6301 aattaggttt gtatttgctg gaaattctgg atgaagtgta ttactcattt gtccaactag
     6361 aaaaacattc gttccctcac ctattaagtc atatccagtt tgatcaccat ttccgccttg
     6421 ttgaaagtac tggattaacg accaatcact agcacatcgt agtatatagg aattcgcaga
     6481 atcgccagat gaaatattga atgatgaatc acctaattcg atatcaacat cgcccttgta
     6541 actgcccgtg attaatatag aattagattg cccttgagta attcgattga tagtagctgt
     6601 aagtgtttga cttccttgca ttaatacagc cgaaacaaga actgccgaat tggtataacg
     6661 tgctaaaaat ggagaagcat tactacccgt tttggttaat gtagtttgcg ctacacctgg
     6721 atcaacatcc attgatgtgc tattcatact gcctgcaata actatttgat tgtcttcggt
     6781 catcataact gatgtaccta aatcaagtga gcttcctcct aataagaagc cccaggataa
     6841 agtcccatcg ttctgagaat acttcaccaa aaaagcatct actgcagcag tcagattcgt
     6901 aacacctacg ccaggattaa tatcagcagt tccacctaaa agtccagtac aatacacata
     6961 tccatctgca cttaagcaca tatcaccaat gtagtcaatg gcaggtgttt gtgagccaat
     7021 aacattcatc caaactaatt gcaaatcgct gtaatatctt cccacaaagc aatcacccga
     7081 aaagacatta gatgttttag aatagctacc tccaccaaaa tcaaaatcta ccgtgttagt
     7141 gaattgtcca gcaataaaaa tgtcatcgaa gacatcaatt ctaatactcc gaatatcaat
     7201 ggttcctcca gaagtaatat ttctaaaatc taaaagctgt cctgtttcac catcgtattt
     7261 agccaaatat ccatccaaag aaccagaagg atcagaacct attatttcgg tagaatcgct
     7321 tggatcgaga tcaatactac cttgaaatcg accgctaatc caaatatcac cttctgagtt
     7381 acaagcaata gcagaaacta aatctgtatt tgcacctcca ataccaattt gccataacaa
     7441 cgttcctgtc ggagaatatt tgtttacaac aacatcttga aatccatatg caggtaatgc
     7501 tccaaggcta gattgggttg gtgcagtata cgttccggct gcaaaaacat tgtgatctgc
     7561 atccatagca attcggctta gattatcggc cgaagctcct cccattgcat gtccccaagt
     7621 tagttgctgt gcgtgaatac tcgtgcacaa acagaataac ccagtaacaa ttccccagtt
     7681 tttcatacag caattgtcaa gcgtttgaat gaataaaata aatagtcaaa ctacctgaaa
     7741 ttacgaataa ttcgggaaag tatcgaaaga caattgttca taagtgccct ttaatgattc
     7801 agacactact gaaattaact atcacaactg attagtatcc aataacttgc tgttcaattt
     7861 cagcaggaat ctctcgataa tcgtattgct gagatcgcaa ctgtggctca aaaccagcca
     7921 aacgaatggc ttcttgaata ccagctgaag taaatcgatg tggcgcacca gctgcggaaa
     7981 cgacattttc ttcaatcatg atagatccaa aatcgttggc acctgcatgt aaacacaatt
     8041 gagccgtatt ttctcctact gttaaccaag acgcctgaat attaatcacg tttggcaaca
     8101 tcattcggct caaagcaatc atgcgtatgt attcatcggc agtaacttga ttgcgtacat
     8161 ttttaactct acgcaacaac gttccatcat ccataaatgg ccatggaata aatgctaaaa
     8221 agccttttgc attagcaggt ttttcgcttt gaacctgacg aatccaagca agatgttcaa
     8281 atcgttcttc aaccgtttcg atatgtccaa acatcatcgt ggccgaagtg gtaatatcta
     8341 cttgatgagc tgcgcgcatt acatcgagcc attcttttcc actgcatttt cctttcgaaa
     8401 tcatccgcct tactcggtca ttgagaatct ccgctcctgc tccaggcaaa gaatctaatc
     8461 cggcagcttt taaccttact aaaacttcgg tatgagtaag ctgactgagt ttagcaatat
     8521 gagcaatctc aggtggtcca agtgcatgaa gtttaatatc tggaaaatga agctttaaat
     8581 cgcgaaacaa ttgttcgtag aaatcaatgc ctaattcggg atgatgtcca ccttgaagta
     8641 aaagttgatc accacctaag cgacgcattt cttcaatctt aactcggtat tggtcgattg
     8701 tagtgatata cgattcggca tgaccgggaa tacgataaaa gttgcaaaac ttacaatttg
     8761 cgatacatac gttggtagta ttcacattgc ggtcaatcat ccaagttact ttcccgtcgg
     8821 gcttttggcg tttccgaagt tcattcccca caaacatgag ttcgggagtt gatgcttgat
     8881 gaaaaagaaa aactccttct tcgatagtaa gccactccat acgaagtgct ttttccaata
     8941 atgcctgaac gttcatagaa tccataacag gcgcaaggta gaatttgttg agcataccca
     9001 aaagttctgc gttagctaag aataaaattc agtcgatttc ataacattta tcaagaagcc
     9061 ttgttattaa tcattcagaa atttgatttt caatttattc aagcaattca gtgaatccag
     9121 cctataacag gctaacattg ccatgaattg ttacatttgc cacttaaacc attcccatgt
     9181 caaccattag catttcaaga ataactaaag ctggaaaagg cgatcgtgta ctagttgctt
     9241 ctaaaacgga agacttgaaa aatcaaggat tttcgaaaga agaatattca tacatccaag
     9301 ctcaatcgaa actacaacgc aaaagcatcg ccgtgaatca tttcgggcat tttagctttg
     9361 tggttcttat tgacaccaaa aaaactgaat tcctcacatt agaagctcaa cgtgtagcag
     9421 gaaatgcctt g
//
LOCUS       DAOOQW010000076         9287 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_9211_length_9287_cov_6.287803, whole genome shotgun sequence.
ACCESSION   DAOOQW010000076 DAOOQW010000000
VERSION     DAOOQW010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 9287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9287
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9211_length_9287_cov_6.287803"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            583..6159
                     /locus_tag="PK281_05975"
     CDS             583..6159
                     /locus_tag="PK281_05975"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HOY48927.1"
                     /translation="MKTMFSLLSMRLVKVLFVCFTTVGLAGVGVYQYLQPPNLSYVED
                     FAPYISGFTNGTVGKKSTIQVLLNFDHPDEIVPGKVIEEELLEFEPSVKGELKWNDKR
                     TLEFVPSKPLESDQEYYCTFRLGEVLDTLPKKFRKFVFKFRSYKQDFRVDYDGLSPLS
                     NNDPLWQHLSGRIQANDDIDSSGTMKMVTATQDGRDLKIKWDLQEINWGSHLAQFTID
                     SIRRGENAGKVHVEIDGNSIGVDRQDKRDFVVPSMGDFNVQNATVEQYPEQSVLIQFS
                     DPIRNGQQLQGMLRIEGQPEPRIILEDNSIRLFPALKLKGTYKILVSAGIENVWGYRM
                     NKGETVSVEFLPLKPNIRMISQGVILPDAGKLFFPFEAVGLKAVDVQVYKLYENNILQ
                     FLQVNDLNGQDELLRVGKKVLSKKIDLKYDSITDLEFWHRYAIDLGSLIRTEPGAMYR
                     VKLSMQKEYAIWDCPAVNPGDEEEEEYYEEKAPEVDFTQYRQDDGSDENYSWDYAYES
                     DYANNPCEKSYYRERRYGHNVLASDLGVIVKREPNGKLHVVVNNLLTTQPIPNATVLF
                     YNYQQQVIAQGTTDANGMIERTPAIKPFVIIVKSGAQRGYMKLDEGTSLSLSNFDVEG
                     RTSEKGIKGFIYGERGVWRPGDSLYLNFILEDPLHSVPTKHPVTMELMNPMGQVVNKL
                     TRTESVNGFYDFRTATNPEAPTGNWLAQVHVGNRSFTKNIKIETVKPNRLKLYMNFGG
                     KMLKKSAQKDTARLEVKWLHGAVARNLKADVEVSVSQASTTFEKYPNYCFDDALKGFE
                     SRKLTLFDGNLNERGLAIFSTKLDAGQSAPGFLRANFFTRAFEEGGDFSTDRFSILYS
                     PYSSYAGIRVPESNGRRDVLETGKENTIQIASVREDGTPGTSRLQVKVYKLNWQWWWD
                     RYDDDINYYLSRPGIYPVYDSTYSTSNGKADLKLNFDEKSWGRYLIRVTDLTSGHASG
                     KVVYFESPWWQRGNSAGTQFASMLSFSSDKESYRAGEKVKLSIPSAGKGKALISIESG
                     ERILQKFWVDTEKGETHAEFTCTADMAPNAYVFVTLLQPHGSVQNDLPMRLYGIIPLS
                     VEDPATHLEPLIQMADVLVPESTAKITVKEKNGKAMTYTLALVDEGLLDLTRFQTPQP
                     WDEFYQKEALRVKTWDLYDWVIGAYGGKLNRILSIGGDGSGLNGKQAKANRFKPVVRF
                     VGPFYLPAKGSQTHSLNLPQYVGSVRVMVVAGNAGAYGNAEKTVPVKKPLMVLATLPR
                     VVGPDESISLPVNVFAMEENIKNVSVQVEVNNLLVIDGSAVQSTHFDFPGDQVLNFKM
                     KVAQRDGIARIKVTAKCGKETAIQDFEVDVRPSNAPFTEINRVKLDPGNEVSLDLPFT
                     GLDGSHSLSLESSLLPVDISKRMNYLIQYPHGCIEQTTSSAFPQLAMQSVMDLSAKEQ
                     IKITNHIKAALNRIALFQTHSGAFSYWPGEGTASEWGTNYAGHFMLEAERLGYTLPVG
                     MKNRWVKYQVNEAQNFSETRITSSEQLTQAYRLYTLALSGSPELGAMNRLRETKILNL
                     NARWRLAAAYAQCGQPEIARLLIKNQPNRIKAYKELSNTYGSEGRDEAMILETMLLLN
                     DRLQADKLVDKLVKEINSNSWMSTQTTAYNLIAIGKYMSNQTASSATSFSYVMDKNAS
                     VSRIQSKPASKEILIQNADRSAHRITLKNEGRSTVYIRLVREYIPLQGDEPTVNKGMK
                     LLWAFKDLDGKYISPDKLQQGTDFISEVTVVNPEQRYLQEMTLNQIFPSGWEIRNVRM
                     EESGQLQSDAARYQDYRDDRVYTYFDLSGMNKRTYRILLHATYKGRFYLPGIECEAMY
                     DREIYARVKGRWVEVF"
     gene            6272..6637
                     /locus_tag="PK281_05980"
     CDS             6272..6637
                     /locus_tag="PK281_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013684945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HOY48928.1"
                     /translation="MSKLSNNPEVDAYIATFPEKTQVQLQNIRTIIRQVIPQATEVIS
                     YKMPAYKLKGVVVYFAGYTNHIGFYPTGTGIAKFQHEFGNYKSSKGAVQFPIEQPLPE
                     DLIQRIVAFKAQEDELKKK"
     gene            complement(6766..>9287)
                     /locus_tag="PK281_05985"
     CDS             complement(6766..>9287)
                     /locus_tag="PK281_05985"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOY48929.1"
                     /translation="FLFLSITRLSLHQYCACEMIVKRVGFNYFLFLFLIGISNVVQAQ
                     SIRVLGRIFDATSSKPVEFAIVSALSKSDSLLGGGLSQGNGDFMLENISRQASQIRIQ
                     FIGYETQVIPLKTVASVSLYDLGNIRLKVNQQQLKELVIEGEKSQMTLGIDRRIYQVD
                     KDLSARGGTALDAMKNIPGITVGSDNSVQLRNQGPTIFIDGRPSLLSLDQIPADDIDR
                     IEVITNPSVKYSASTTGGILNVVLKKNLRPGYFGSLSAGIGSNNRYSTNGNISIREKK
                     LSIQVSGNYGASQNQNNGYTHRENFSNGQTAGKFNLDNTNLTSRWNSSGRIAADYKIN
                     VRSLLSANYNYSENGVDANENQNYSTSDSSTQIINSGYRNNELKNSWKVHSAQILFKK
                     NYPKIGKELSADIGMTHSDNYNYSLFDTHTTLDLLNPDFISNEKQNNTGNRSATLYNG
                     QIDYSNPLTSNSRLDWGGRFAYKQSFSDFEVNTLDPNTNISISDTGLSNKFRVDDFIG
                     AAYVNYAGKLGPFMYQAGMRYEHTWFVAESQKSGQEYSYIYPKSVSDLDKVLFPAIYF
                     SKKIKENHEFQLNFSRKIGRPGYMQLIPFITYADRQSVQIGNPVLGPEFINLAELNYS
                     FQGNKSSVLSGIYTRQTLQAITTVLYKSPTNPDILVSSFGNGKDKFDLGWENTFKQKL
                     FTWLDFSLNTNVFYSKTSLEQNGILSQNEGLSWNAKSMFSVKATKKLSFQINGSYEAP
                     RIIPQGTINAIWFSDISCNININKKLSASATLSDVFNTKRYGTNYQSASFNQDIIRRW
                     ETRYFRMNITWKFGEPDVSLFRRRNNSRREPGSGGTEMQEL"
BASE COUNT         2869 a         1580 c         1953 g         2885 t
ORIGIN      
        1 taaaattaag atttattcct aattttaacc agtctcgatt ttaggggtac ttacatcttg
       61 tgatattaaa ttaccggtca caaaaaatga gaataataat atcgaagcga attctttttt
      121 tgaggttaac atagatttca aattattgct caaatttatt ttgatatgat ttgtttgaaa
      181 agagttttat agtcgtttcg aatagtgatt ttaatagtct ttaaataggt tgttttcacg
      241 tattcttaat ttgtagctta taatcgatag tactataatc cccgcctgag cgattgaagc
      301 gtaaagcccg caacgtatgc cttcgcttta gctacggcat aaagtgagga cttgcagcgg
      361 aaagcgcgac ctgttgaatg aagcgatagc ggaatgaata aagggcaggt cataattgat
      421 gtgctgatgt gctggttaga tgatttgctg gttagatgat atgatggtta gatgatatga
      481 tggttagatg atttaaatat acagcctaac gaattctcgc tcgtttcctg cacttactca
      541 atccacctga tctatacaga tacagtggtg tagtcttgct gcatgaaaac gatgttctct
      601 ttgctctcga tgcgcttggt taaagtgctt tttgtttgtt ttactacggt tggacttgct
      661 ggtgtaggtg tttaccagta tctacagcca ccgaatcttt cgtatgtgga agattttgct
      721 ccttacattt ctggttttac caatggtact gttgggaaga aatcaactat tcaggtgctt
      781 ctcaattttg atcatccgga tgaaatagtt cctggaaaag tgattgagga agaattgctc
      841 gaatttgagc catctgttaa aggggaactg aaatggaacg ataaaagaac actcgaattt
      901 gttccttcta aaccactcga aagtgatcaa gaatattatt gcacgtttcg actaggagaa
      961 gtgctggata cgctgcccaa gaaattccga aaattcgtat ttaaattccg ttcgtataaa
     1021 caagattttc gggtcgatta cgatggatta agtccgcttt ctaataatga tccgctctgg
     1081 caacatttaa gtggtcgcat acaagcaaat gatgatatcg actcatctgg taccatgaaa
     1141 atggttactg ccacgcaaga tggccgtgat ttaaaaatta aatgggattt acaggaaatt
     1201 aattggggaa gtcacttagc tcaatttacc attgatagta ttcgccgtgg ggaaaatgcc
     1261 ggaaaagtgc atgtcgaaat tgatggaaac tccattggtg tggaccgaca agataaacgc
     1321 gattttgtgg tgccttcgat gggtgatttt aatgtgcaaa atgccaccgt agaacaatat
     1381 ccagagcaaa gtgtactcat tcaatttagc gatccgattc gtaatggaca acagttacaa
     1441 ggtatgcttc gaattgaagg acaaccagaa ccgcgcatta ttttagaaga taattccatt
     1501 cgattatttc ctgcgttaaa attaaaagga acgtataaaa ttttggtgag tgcgggaatt
     1561 gaaaatgttt ggggataccg catgaataaa ggagaaacag tttcggttga atttcttcct
     1621 cttaagccta atattcgaat gattagtcaa ggagttattt tacctgatgc tggaaaattg
     1681 tttttcccat ttgaagctgt gggcttgaaa gctgttgatg tacaagtgta taaactgtat
     1741 gaaaacaaca tcctacaatt tctacaagtc aatgatttaa atgggcagga tgaattatta
     1801 cgtgtaggta aaaaagttct ttccaagaaa attgatttaa aatatgattc aatcacggat
     1861 cttgaatttt ggcatcgtta cgccattgat ttaggaagtc tcattcgcac cgaaccggga
     1921 gctatgtatc gtgtgaaatt atcgatgcag aaagaatacg ctatttggga ttgtcctgca
     1981 gtaaatccag gtgatgagga agaagaggaa tattacgaag aaaaagcacc ggaagtagat
     2041 ttcactcaat accgacaaga cgatggttca gatgagaatt attcctggga ttatgcatat
     2101 gaaagtgatt atgcaaataa tccatgtgaa aagagttatt accgcgaacg tcgctacgga
     2161 cacaatgtgc ttgcatctga tttaggtgta attgtgaaac gcgaaccaaa cggaaagcta
     2221 catgtggttg taaataattt gctcactact cagcctattc cgaatgcaac ggtattattc
     2281 tataattatc agcagcaagt tattgcacag ggcacgacag acgcaaatgg aatgattgag
     2341 cgaactccag ctatcaaacc ttttgtgatc atcgttaaaa gtggcgctca gcgtggttat
     2401 atgaagttgg atgagggtac gtcattatcg ttgagcaatt ttgatgtaga aggacgaaca
     2461 tccgaaaaag gaattaaggg atttatttac ggtgagcgtg gtgtttggcg tccgggtgat
     2521 tctttgtatt tgaatttcat tttggaagat ccgctccaca gtgttccaac caaacatccg
     2581 gttacgatgg aattgatgaa tccgatggga caggtagtga ataaactaac tcgaactgaa
     2641 tcggtgaatg gtttctatga tttcagaacg gctactaatc ccgaagctcc aacaggcaat
     2701 tggttagcac aggttcatgt gggaaatcgc agttttacta aaaacattaa gattgaaacg
     2761 gttaagccga atcgcctgaa attatatatg aattttgggg gaaagatgtt gaagaaatcg
     2821 gctcaaaaag atacagcacg tttagaagta aaatggttgc atggcgcagt tgcacgtaat
     2881 ttaaaagcag atgttgaagt gagtgtatcg caagccagta ctacattcga gaaatatcct
     2941 aattattgtt ttgatgatgc attgaaagga tttgaatcgc gtaaattaac tttgtttgat
     3001 ggtaatttaa atgaacgtgg attagcgatt ttctccacca aattagatgc aggacaatcg
     3061 gctccgggct ttttaagagc aaactttttt acccgtgctt ttgaagaagg tggtgatttt
     3121 agcaccgatc gtttttctat cctttattca ccttattcaa gctatgcagg tattcgtgtt
     3181 cctgaatcga atggtcgtcg cgatgtgctc gaaaccggaa aagaaaatac cattcaaatc
     3241 gcatctgttc gtgaagatgg tactccagga acatctcgtt tgcaagtgaa agtgtacaag
     3301 ctcaattggc agtggtggtg ggatcgttac gatgatgata tcaattatta cttaagtcgc
     3361 ccggggattt atccagtgta tgattcgacc tattcaacta gcaatggaaa agcggatttg
     3421 aagctcaatt ttgatgagaa atcttgggga cgttatttga ttcgagtaac ggatttaaca
     3481 tcgggacacg ccagtggaaa agtggtgtat tttgaaagtc cgtggtggca gcgtggaaac
     3541 agcgctggga ctcaatttgc aagtatgttg agtttctcaa gcgataaaga aagctatcgg
     3601 gcaggggaga aggtgaagtt gagtattcca agtgcgggta aaggaaaagc cttgatttcg
     3661 attgaaagtg gggaacgcat tcttcaaaag ttttgggttg ataccgaaaa aggagaaaca
     3721 catgccgaat tcacctgtac agctgatatg gcaccgaatg catacgtatt tgtgactttg
     3781 ttgcaaccac atggaagtgt gcaaaacgat ttacctatgc gtttgtacgg aattattcct
     3841 ttgtcggttg aagatcctgc cacgcattta gaaccactca ttcaaatggc tgatgtgctt
     3901 gttcctgaaa gtaccgctaa aatcaccgta aaggaaaaaa atggcaaggc aatgacatac
     3961 acgcttgcat tggtagatga aggtttgctc gatttaacac gctttcaaac tccacaacct
     4021 tgggatgaat tttatcaaaa agaagcatta cgtgttaaaa cctgggattt atacgattgg
     4081 gtaattggtg cttatggtgg aaaattaaat cggattttaa gtattggtgg cgatggttcg
     4141 ggattgaatg gaaaacaagc gaaagccaat cgtttcaagc ctgtggtgcg ttttgttggt
     4201 ccgttttatt tgcccgcaaa agggtcacaa actcattcct taaatctccc tcaatatgta
     4261 ggttcggttc gggtgatggt tgttgcggga aatgcaggtg cttatggcaa tgccgagaaa
     4321 accgttccgg tgaagaaacc attgatggta ttggctacac tgcctcgtgt tgttggtcca
     4381 gatgagtcga ttagtttgcc tgtaaacgta tttgctatgg aagaaaatat taaaaatgtt
     4441 tccgttcagg tagaggtaaa taatttatta gtgatagatg gttcagcagt tcaatcgact
     4501 cattttgatt ttccgggaga tcaagtattg aattttaaaa tgaaagtcgc tcaacgtgat
     4561 ggtattgctc gtattaaagt tactgcgaaa tgtggaaaag aaacagcgat acaagatttt
     4621 gaggtggatg ttcgtccttc gaatgctcca tttacggaaa taaatcgggt gaaattggac
     4681 cctggaaatg aagtgagttt ggatttacct tttacgggtt tagatggaag tcattcgctt
     4741 agtttagaat cttcgctttt gcctgttgat attagcaagc ggatgaatta tttgatccaa
     4801 tatccgcatg gttgtattga gcaaactact tcatcagcat ttccacaatt agcgatgcag
     4861 tcggtgatgg atttaagtgc gaaagaacaa attaaaatca ctaatcatat taaagctgct
     4921 ttgaatcgaa tcgctttatt tcagacacat tcgggagctt tttcctattg gccgggtgaa
     4981 ggaacagcat ctgaatgggg cacaaattac gctggacatt ttatgttaga agctgagcga
     5041 ttgggatata ctcttcctgt tgggatgaaa aatcgttggg tgaaatatca agtgaacgaa
     5101 gcacagaact tttctgaaac ccgcatcact tcaagcgaac agctaacaca agcttatcgt
     5161 ttatacacct tggctttatc tggtagtccc gaattaggag cgatgaaccg tttaagagaa
     5221 acgaaaattc tgaatctcaa tgctcgttgg cgattagctg ctgcttatgc acaatgcgga
     5281 caacccgaaa ttgctcgttt attaattaaa aatcaaccca atcgaattaa ggcttataaa
     5341 gaactttcta atacatacgg tagtgaaggc cgagatgaag ccatgatact ggaaaccatg
     5401 ttgttattga atgatcgttt gcaagctgat aaattggtcg ataagctggt gaaagaaata
     5461 aatagcaact cgtggatgag cacacaaaca actgcctata atttaattgc cattggaaaa
     5521 tacatgagca atcaaacagc tagtagtgct acatctttca gttatgtgat ggataaaaat
     5581 gcttcagttt ctcgcattca atctaagcca gcttctaaag aaatcctgat tcaaaatgca
     5641 gatcgttcag cacatcgaat tactttgaaa aatgaaggta gatcaaccgt ttatatacgt
     5701 ttggtacgtg aatatattcc tttgcagggc gatgaaccta ccgtgaataa aggcatgaag
     5761 ctactttggg catttaaaga tttagatgga aaatatatta gtccagataa gcttcagcag
     5821 ggaacagatt ttatttctga agtcactgtg gtaaatccag agcagcgtta tttacaggag
     5881 atgaccttga atcagatttt tccttcggga tgggaaattc gaaatgttag aatggaagaa
     5941 agtggacaat tgcaaagcga tgcagcacgc tatcaagatt accgagatga tcgtgtgtat
     6001 acctacttcg atttatcggg aatgaataaa cggacatatc gaattttgtt gcatgctaca
     6061 tacaaaggac gattttacct gcccggcata gagtgtgagg cgatgtatga tcgagaaatt
     6121 tatgcacgag taaaaggtcg ttgggtggaa gtattttaag tggaatagaa aaggaaataa
     6181 cgctcttaga tttagtttaa gagcgttatt ttttatttgc cgtatttaat tcttgattat
     6241 tttagagcca tgtttttcaa ttattagaag gatgagcaaa ttatcaaaca acccagaagt
     6301 ggatgcttat atcgccacat ttccggagaa aacgcaggtg caattacaaa acattcggac
     6361 aataatccga caggttattc cacaagcaac agaagtaatt agttataaga tgccagctta
     6421 taaattaaaa ggtgtagttg tttattttgc tggttatacc aatcatattg gcttctatcc
     6481 aaccggaaca ggcattgcta aatttcaaca cgaatttggt aattataaaa gttctaaagg
     6541 tgccgttcaa tttcccattg aacaaccctt gccagaagat ttaatccaac gaatagtcgc
     6601 ttttaaagct caagaagatg aattgaaaaa aaagtagtac gatatacgta ttacgattaa
     6661 cgaaaattac ttagaataat aaattacgaa ttacgaatta cgaattacga attacgaatt
     6721 acgaattacg aatttgatta aataaatcgt acatcgtata tcgatttaca attcctgcat
     6781 ttctgtacct ccactaccag gttcacggcg tgaattattt cttcgtctaa ataaggaaac
     6841 atccggttcg ccgaatttcc acgtgatatt catacggaaa taacgcgttt cccaacggcg
     6901 aataatatct tgattgaaac tagcactttg ataatttgta ccgtatcgtt tcgtattaaa
     6961 gacatcgctt aaagtggctg atgcagatag ttttttattg atgtttatat tacaggaaat
     7021 atcagaaaac caaattgcat taatagttcc ttgtggaatg atacgaggag cttcgtaact
     7081 cccattaatt tgaaagctta gttttttagt tgcttttaca ctaaacatcg actttgcatt
     7141 ccagcttaat ccttcatttt ggctgagaat accattttgt tctaagctag tcttgctgta
     7201 aaatacattt gtgtttaaac tgaaatccaa ccaggtaaac agtttttgtt taaatgtatt
     7261 ttcccaacct aagtcaaatt tatctttccc atttccaaac gaactaacca aaatatcagg
     7321 attggtgggt gatttatata atacagtagt aattgcttga agtgtttgac gtgtataaat
     7381 cccacttaat acactgcttt tatttccttg aaaactataa tttaattctg ccagattgat
     7441 gaattcaggt cctaaaaccg gattgccaat ctgaacactt tgtcggtctg cgtaggtgat
     7501 aaatggaatt aattgcatat atcccggacg accaatttta cgagagaaat tcaactgaaa
     7561 ttcgtggttt tctttgattt ttttagaaaa gtaaatagct gggaatagta ctttatccaa
     7621 atcacttaca gattttggat agatgtaact gtattcttgt cccgattttt gagattctgc
     7681 tacaaaccaa gtatgctcat atctcattcc cgcctgatac ataaaaggac ctaattttcc
     7741 tgcataattc acatatgccg caccaataaa atcatctacc cgaaatttat tggaaagtcc
     7801 ggtatcactt atactaatat tggtattggg atctagcgta tttacttcaa aatcggaaaa
     7861 ggattgttta taggcaaatc gtccacccca atcgaggcga gaattggatg ttagcggatt
     7921 ggaataatca atttgtccgt tatataatgt agcagatcga tttccggtat tgttttgttt
     7981 ttcattggaa atgaagtctg gattcaacag gtctagcgtg gtatgcgtat caaacaagga
     8041 ataattataa ttatccgaat gtgtcattcc tatatcagcg cttaattcct taccaatttt
     8101 aggatagttt tttttgaaga gaatttgagc tgaatgaacc ttccaactat tttttaattc
     8161 attatttcgg tatcctgaat tgattatttg agttgaacta tcgcttgtag aatagttttg
     8221 attttcgtta gcatctacac cattttcaga ataattgtaa ttcgcactga gtaaggaccg
     8281 aacattaatt ttatagtccg cggcaatacg tccgcttgaa ttccaacggc tagttaaatt
     8341 ggtattatct aaattgaatt tccctgctgt ttgtccattg ctgaaattct cacgatgagt
     8401 atatccatta ttctgatttt gagaagctcc ataatttcct gaaacttgga tgctcaattt
     8461 cttttctcga atactgatat tcccgtttgt tgaataacga ttattgctac caattccggc
     8521 acttaatgag ccaaagtagc ctggacgaag gttcttcttc aatacaacat ttaaaatccc
     8581 tccagtagtg cttgcggagt atttaacaga tggatttgtg atgacttcaa tccggtcaat
     8641 atcatcggct ggtatttgat ctaggctgag taaactgggg cgaccatcga taaaaatggt
     8701 tggtccttga tttctcagtt gtactgaatt atcgctacca acagtgattc caggaatatt
     8761 tttcatggca tcgagtgccg ttcctccacg ggcgcttaaa tctttatcaa cctgataaat
     8821 tcgtcgatca atacccaaag tcatttgaga tttttcacct tcaatgacaa gctctttgag
     8881 ttgttgctga ttgactttca atcggatatt tcctaaatca tatagtgaaa cagaagctac
     8941 cgttttaagt ggaatgactt gcgtttcata accgataaat tggattcgaa tttgacttgc
     9001 ttgacgagaa atattctcca acatgaaatc accatttccc tgactcaaac caccgcctag
     9061 caagctgtcg gattttgaca atgctgaaac gatagcaaat tcaactggtt tagaagatgt
     9121 tgcatcgaat attctgccta aaacacgtat tgattgtgcc tgaacgacat tcgaaatgcc
     9181 aatcaagaag aggaacaaga aatagttaaa tccaacccgt tttacaatca tttcacaagc
     9241 acagtattgg tgcaaagaaa gtcgggttat acttaagaac aaaaata
//
LOCUS       DAOOQW010000077         8914 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_9692_length_8914_cov_3.948414, whole genome shotgun sequence.
ACCESSION   DAOOQW010000077 DAOOQW010000000
VERSION     DAOOQW010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 8914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8914
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9692_length_8914_cov_3.948414"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            14..1087
                     /locus_tag="PK281_05990"
     CDS             14..1087
                     /locus_tag="PK281_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013069637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HOY48930.1"
                     /translation="MSEKPYTKGRGAQINPANRFLKLRQEVDVEHQSSNEEREALLNE
                     NPATKYLDVFPKTIINKVESTDLPDFSLNPYQGCEHGCVYCYARNTHEYWGFSAGADF
                     EQNILVKRNAPELLRKTLADPKWKADLIMMAGNTDIYQPIERKLKITRELLEVFWKFR
                     HPVGMITKNSLIERDIDILEKLASENLVNVLLSLTTLDEQLKRKLEPRTSSAKNILKT
                     IETLTNRGIPVRVLTAPIIPSINDHEIHDLVKAIADAGALGVSYQVVRLNGQVALLFE
                     DWLNKNFPDRANKVLNQIRSVHGGQLNDSRFGVRMRGEGLWADMIRKQFKLATQAYLK
                     DRKIPSLNFELHDQFKGGQMRLF"
     gene            1193..3271
                     /locus_tag="PK281_05995"
     CDS             1193..3271
                     /locus_tag="PK281_05995"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY48931.1"
                     /translation="MNPIRIQASLLSGLWKPMLLVGTLLFSQSGNAQTQVLVPEDIVA
                     NDFANNAVGGWRILSKGNSNCSLVAVADYGISQNSGPHSLRATRTGGGGVNRSFVGYY
                     EEGRTLNSLSSVSWNQYTEQGSDSYLNIFLTDGFSVATVIYQPATVLNAWTTHTFNSN
                     SSTSVLSIRVGSTMTPITYTDLMNQYGSWLIYNHPLTFVFPNSATDFIGGIVLVSGSS
                     NPTLPQTHTFDKASISYTGEAPLVFDFATSTPPPPACTAVSVVSYEPGNRQDGTPVLP
                     TRQFFNNATSAPQNSDVAVPEDALNFVSLGFGGQIVLELEVPIANGEGDDFMVYETTY
                     LPSTIEKCKRSPETIKAYASQDGCNFVYLGEGCQDVSFDLRSLAWARFIKLVDVSPIG
                     VVFTNGNNGPEDGFDLDGIECLNGMATDLTPTSLVPAVLTQVIDHTPDSQARRKNNTL
                     IINGTPERRVVTRMLGVPENNNTVNFYSMGFGGFVVVKFDFVVFNGVGNDLKVYETSY
                     GNPSCTNYPERANVEVSLDGATWLDLGTYCQDGLLDIAATGFQGIQYVRLVDRSPVSS
                     TKFPGNADAYDLDGIVDLHACTSTSTSRLADNSGEEVIEGLPNSASIYPNPFEDKVTV
                     SFINESNDKEVSISVYNLVGALVYSKNYAVESEAFFNQTIDLSELPAGLYTIHTACPS
                     FMQTDKILKR"
     gene            complement(3412..3972)
                     /locus_tag="PK281_06000"
     CDS             complement(3412..3972)
                     /locus_tag="PK281_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48932.1"
                     /translation="MHSLRVELLDHLCCHIEAQLDSGISFELAFKEAILQLSPEGLSA
                     VEERTVFLLTFKKQLTMKKLLYSTGFLASLFTMTSFTFRTFHWPGGSAIMFMGNVFLL
                     LSMIFILIIAIRNYSLITGNARVRSLSGAIGGIVVAVGSMFKILHYPGANILFLIGTV
                     ILICVFLPILFFQLYKNDVAQSSEAV"
     gene            complement(4016..4369)
                     /locus_tag="PK281_06005"
     CDS             complement(4016..4369)
                     /locus_tag="PK281_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013684875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOY48933.1"
                     /translation="MYSKELLKGTLSTILLNLLDENGRMYGYEITQAVKERTDGKILL
                     KEGSLYPALHKLEAEGFLSTEEEFIGKRPRIYYRLTSTGKAQSRIRVDELFQFFETLQ
                     QVLIPKPGLQYGSAG"
     gene            4544..6322
                     /locus_tag="PK281_06010"
     CDS             4544..6322
                     /locus_tag="PK281_06010"
                     /inference="COORDINATES: protein motif:HMM:NF022525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2779 domain-containing protein"
                     /protein_id="HOY48934.1"
                     /translation="MSVLLNKSDFKLARSCYRKLIYRKQGFPSTNDRNRFLALLADSG
                     LVVQALARLYFPQGILPPDSNTALNWTQQAMQLETVTLFEAPFETASRHIRADILIKQ
                     GNKIQLIEVKSRGVDVEEINGNPHRRRRALSKIKDLLEDLAYQKLVIQACHPDFEISC
                     HLMMPDKNRVNPIDRLADRFRIKSNTDNQYHTFIDAEYFGNVDEVLSANLLTQIDVTF
                     EVDALLDELDSEAYKLEILLQNPEKIEPEIGCHCRDCEFSVRSENQPLSGFELCWKAG
                     AHTEPHILSLGQLGNVNLHGEVDELIAQGESSIHAIPEMWLKGKWGNRPWLQLTQQRD
                     FMFEGFHKSISGLSYPMFFIDFETTQPAIPNYQGMKPYQKVLFQWSCHRVDFPGAEPV
                     HTAWLHDSKEKPNAEFLSSLIAQLGEKGTIMTWSAYEQGILKGLLSEIEITDAQKDWI
                     RRAFHRITDMNQLAVHYYFHPQTGGRTSIKVTLPAILKSVRSKRTVAWLKELDVYKLD
                     ENQELINPYYLLPELGLPGTEGSINDGSGAMIAYQYLQLSIENRESETYIQYRNALLR
                     YCQLDTLAMLIIYEEWLLMEESKMPS"
     gene            complement(6324..7610)
                     /locus_tag="PK281_06015"
     CDS             complement(6324..7610)
                     /locus_tag="PK281_06015"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOY48935.1"
                     /translation="MRPLRYFEHLDTLRFFAFFSVFCLHMTSFGLYYNGSAWYRWIEE
                     SLAQNGDLGVNFFFTLSGFLITNLLLQEREDTGKINIGAFYARRFLRIWPLYFILVFV
                     GFVILPGLLKLHPESNPFPPVFSIIPIDRIGYYLSFLVNFDITMHGYPSPVLAVLWSI
                     SVEEQFYIFWPLLIALVPRKYLIYSLFGVLIFTAGFRFMQWNEIVHLFNTYSLGDPSK
                     PHPVDVVNRFNELRNHAMLVTRFHTLSILSDITMGALLAYGLFYSEKLRQYFRTLPRK
                     SIVLPYIAAIVLIIIRDRTYSFQFYPIMNWVQAIEPAIFSVIFAFIIGEQEYSEESFF
                     KFGKIKLLSSWGKLSYGLYLWHLVMIWLIFYLFKTSGLQPAVDSPWVYLFQIILCLFG
                     TIVFSSLSYRFIESPFLKLKDRFRATKPNPSAGADA"
     gene            complement(7727..>8914)
                     /locus_tag="PK281_06020"
     CDS             complement(7727..>8914)
                     /locus_tag="PK281_06020"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY48936.1"
                     /translation="WGNADCWNIPDWEDGTTEPGSSGSPLFNENHLIIGQLFGGDANC
                     NDNVNDNFGRFVTSWDGASANVRLKDWLDPNNSDSLTVSGMEASVPSFSLDARMQSII
                     SPVQEYCNTASFIPSLKIRNTGSTTLTSINISYTFNGGAAQTFDWTGTLAYNQVVDVV
                     LPEQLLSIGTNQNFSATIISVNGATDEQSNNNSLNINVNNRAGAGYRFELVSDEYPEE
                     TSWELVNVATNQIITQQAIGAVSLGTSNFPLCLANGCYSLRMHDSYGDGICCGSSGNG
                     SFTVYDTLGISIGTGGNFDDSDSITFCVGPTSLDFIPESMLIQLFPNPAQENISLLIS
                     EIWLEENPSIKIYSVSGQVIWTGKANQNLTQIDVASWSNGVYLIQAQSKSRILTKRMV
                     VQH"
BASE COUNT         2936 a         1669 c         1684 g         2625 t
ORIGIN      
        1 gattcaatta gacatgtcgg aaaaacccta tacgaaagga agaggcgcgc agattaatcc
       61 ggcaaatcgc tttttgaaat tgcgtcagga agtagatgtg gaacatcaat cgagcaacga
      121 agaacgcgag gctttgctca atgaaaatcc agctaccaaa tacctggatg tttttcctaa
      181 aaccatcatc aataaagttg aaagtactga tttacctgac tttagcttaa atccttatca
      241 aggttgcgaa catggttgtg tgtattgtta tgctcgaaac acgcatgaat actggggatt
      301 tagtgctgga gccgattttg agcagaatat tctcgtcaaa cggaatgcac ctgaactatt
      361 acgaaaaacc ttagccgatc caaaatggaa agctgattta atcatgatgg ctggcaatac
      421 cgacatctac caacccattg agcgtaaact caaaatcacc cgagaattat tggaagtgtt
      481 ttggaaattt cgtcacccgg tgggaatgat taccaaaaat agccttatcg aacgtgatat
      541 tgatattctt gaaaagttag catccgaaaa cttggtaaat gtactactca gcttaaccac
      601 cttggatgaa cagctaaaac gtaagcttga accacgtact tccagtgcta aaaacatact
      661 taagacaatt gaaacactta ctaatcgagg gattccggtt cgagttttaa ctgcacctat
      721 aattccttcc ataaatgatc atgaaatcca tgatttggta aaagcaattg ctgatgcagg
      781 agctttaggt gtttcgtatc aagttgttcg gctaaatgga caagtagctt tgctatttga
      841 agattggtta aataagaact ttcctgatag agccaataaa gtattgaatc aaatacgttc
      901 ggttcatggt ggacaattaa acgattcgcg ctttggagta aggatgcgtg gtgaagggct
      961 atgggctgat atgataagaa agcaatttaa acttgctact caagcatatt tgaaagacag
     1021 aaagattcca tccctgaatt tcgaattaca tgatcagttt aaaggtgggc aaatgcgact
     1081 tttttaacgt atttgatgat tctatattta ttcatttgat caaatgaatc tgctcaattt
     1141 ctttgtatta ttgttaagca taccatttca atatcaacca aatcaaactc tcatgaaccc
     1201 tatacgaata caagcatcac tattatcagg actttggaag cctatgttat tagtgggaac
     1261 acttctattt tctcaatcag gaaatgctca aactcaagta ttagtccctg aagatattgt
     1321 agcaaacgat tttgctaata atgctgttgg aggttggaga attctatcta aaggaaactc
     1381 taattgttct ttagtagcgg tagcagatta tggtatctct caaaattcag gtcctcatag
     1441 tttgcgtgca actcgtactg gcggaggtgg tgttaatcgt tcgtttgtag gctattacga
     1501 agaaggacgc accttaaatt cacttagttc ggtatcttgg aatcagtaca cagaacaagg
     1561 aagtgattcg tatttaaaca tattcttgac ggatggcttt tcagttgcaa cagttattta
     1621 tcaacctgca acagttttaa atgcctggac aactcatacg ttcaatagta attcatcgac
     1681 atccgtatta agcattcgag taggaagcac tatgacacca atcacttata ctgatttaat
     1741 gaatcaatat ggtagttggt taatatacaa tcacccatta acattcgttt tccctaattc
     1801 tgcaacagat tttattggag gaattgttct tgtttctggc tctagcaatc caacacttcc
     1861 tcaaacacat acttttgata aagcatcaat ttcatacact ggagaagctc ctttagtttt
     1921 cgattttgct acttccactc caccgccgcc agcttgtaca gctgtttcag ttgttagtta
     1981 tgagccgggg aatcgtcaag atggaacacc tgttttacct actcgccaat tctttaataa
     2041 tgctacctct gctccgcaaa atagtgatgt agctgttcct gaagatgcat taaactttgt
     2101 atctcttgga tttggtggac aaatcgtatt ggaattagaa gttccaattg ctaatggaga
     2161 aggcgatgat tttatggttt atgaaactac ctatcttcct agtacaattg aaaaatgcaa
     2221 acgaagtcca gaaacaatta aagcatacgc ttctcaagat ggttgtaatt tcgtttatct
     2281 aggtgaaggt tgtcaggatg tttcatttga tttaagatca cttgcttggg cacgattcat
     2341 taaattagtt gatgttagtc caattggagt tgtgtttacc aatggtaata atggtcctga
     2401 agacggtttt gacttagatg gaattgagtg tttaaacgga atggcaacag acttaactcc
     2461 aacatcactt gttcctgctg tgttaactca agtaatcgat catactccag attctcaagc
     2521 gcgtagaaaa aataatacac ttatcatcaa tggcacacct gaacgtagag ttgtaacaag
     2581 aatgcttggc gttccagaaa acaacaatac agttaatttc tactcaatgg gatttggtgg
     2641 atttgtagta gtgaaatttg attttgttgt atttaacgga gttggaaatg acttgaaagt
     2701 ttatgaaacc tcttatggta acccatcttg tactaattat cctgaacgtg ctaatgtgga
     2761 agtttcgctt gatggtgcaa cttggttaga tttaggaacc tattgtcaag atggattact
     2821 tgatattgct gctactggat tccaagggat tcaatatgta cgcttagtcg atcgttcacc
     2881 tgtttcttca acaaaattcc ctggaaatgc tgatgcatat gacttagatg gaattgttga
     2941 tttacatgct tgtacttcta ctagtacctc tcgcttagcc gacaattctg gagaagaagt
     3001 aattgaaggc ttgccgaaca gtgcgagcat ttatccgaat ccttttgagg acaaagtgac
     3061 tgttagtttc atcaatgaaa gcaacgataa agaagttagc atttcggttt ataacctagt
     3121 aggtgcttta gtttattcga aaaactatgc tgtagaatcg gaagcatttt tcaatcaaac
     3181 aattgactta tctgaactac ctgcaggttt atatacaatc catacagctt gtccgagctt
     3241 catgcaaacc gataaaatat tgaagcgata attcaatttt caaaaacgca aaagcccatt
     3301 cttatcgaat gggctttttt tatacctaca agttgcggca ggcaagttca atcgggaaat
     3361 tgagtatcac aaacacatat tcactaaact ccgaaagaac taaaactaac gttaaacagc
     3421 ttctgaggat tgagcaacgt cgttcttata caattgaaaa aacagaattg ggagaaacac
     3481 acaaattaag attactgtac caattagaaa tagaatgttt gctcctggat aatgaagaat
     3541 tttaaacatc gaaccaacag ctacaactat tcctccaata gcaccactca aacttctaac
     3601 gcgagcattg cctgttatca gagagtagtt tcgaatagct atgatgagaa taaaaatcat
     3661 cgataaaagc agaaaaacat tgcccataaa cataattgcg ctgcctcctg gccaatgaaa
     3721 tgttcgaaag gtaaatgaag tcatggtaaa taatgacgca agaaatccgg tagaatacag
     3781 gagctttttc atagtaagtt gttttttaaa cgtgagtaaa aagactgttc tttcttcaac
     3841 agctgacaaa ccctcgggcg acaactgtag aatagcttcc ttaaaagcca attcgaatga
     3901 aattcctgaa tcgagttgtg cttcgatgtg acaacacaaa tgatcgagca attcgacccg
     3961 aagtgagtgc atgctaactg accggttcac cagcaatgac acttgttcaa attgatcatc
     4021 ccgcagatcc atattgcaat ccgggtttag gaattaacac ttgttgaagg gtttcgaaaa
     4081 actggaaaag ttcatccaca cgaatccgag attgcgcttt cccggtcgat gttaatcggt
     4141 aatagatgcg cggtcgctta ccaataaact cttcttctgt acttaaaaaa ccttcagcct
     4201 ctaacttgtg taaggctgga tacaaggagc cttctttgag caaaattttt ccatcggtgc
     4261 gctctttcac cgcctgtgta atttcatatc cgtacatacg accattctca tccaacaaat
     4321 tgagaagtat ggtgctgagt gttcctttta aaagttcttt cgaatacata ggacaaatat
     4381 acattgaact tctatgcatt tagcgtctaa gtattggttt tcgtatattc tagtttgaaa
     4441 aaaggcgcat ctatctgaat atctgaatat taattttatg taaaatatct aacaccgcta
     4501 ttattttctt atcaagccag ataaatcagc tatttttgaa aacgtgtctg tcctactcaa
     4561 taaatctgat ttcaaattag ctcgctcctg ttaccgtaaa ttgatttacc gtaaacaagg
     4621 ttttccttct actaacgaca gaaatcgctt tctagcatta ctcgccgatt caggtttagt
     4681 cgttcaggct cttgctcgct tgtattttcc gcaaggcata cttccacccg attcaaatac
     4741 tgcattaaac tggacgcaac aagccatgca gctcgaaact gtgacacttt tcgaagctcc
     4801 ttttgaaaca gcaagccgac acattcgagc cgacattctc atcaaacaag gcaataaaat
     4861 ccaattgatt gaagtgaaat cgagaggtgt tgatgtggaa gaaatcaatg gaaatccaca
     4921 ccgtcgtcgg agggctttgt cgaaaattaa agatttattg gaagatttgg cttatcagaa
     4981 attggtcatc caagcttgtc atcccgattt tgagattagt tgccacttaa tgatgcctga
     5041 taaaaaccgg gttaatccga ttgatcgact agctgaccgt ttccgcatca aatcgaacac
     5101 cgataatcaa taccatacgt ttatagatgc ggaatatttc ggaaatgtgg acgaagttct
     5161 ttcagcaaat ctgcttactc aaattgatgt aacttttgaa gttgatgctt tattggatga
     5221 acttgattca gaagcttata agctggaaat attacttcaa aatccagaga aaattgaacc
     5281 ggagattggt tgccattgtc gggattgtga attttcggtc cgatcagaaa atcaaccttt
     5341 atcaggattt gaattatgtt ggaaagcagg cgcacatacc gaaccgcata ttctaagctt
     5401 gggacaatta gggaatgtga atttacatgg cgaagtggat gaattgattg cacagggaga
     5461 atcgtcaatt catgcaattc ccgaaatgtg gttgaagggt aaatggggaa atcgcccttg
     5521 gttacagcta actcagcagc gagactttat gtttgaagga tttcataaat ccatttcagg
     5581 actcagctac ccgatgtttt tcatcgattt tgaaactaca cagcctgcta ttccaaatta
     5641 tcaaggaatg aagccctatc agaaagtgct gtttcaatgg agttgtcacc gagtagattt
     5701 ccctggagct gaaccggttc ataccgcttg gcttcacgat tccaaggaaa aacccaatgc
     5761 tgaattttta agttcactga tagcccaatt gggtgaaaag ggaacaatta tgacctggag
     5821 tgcttatgaa caaggaatac tcaaaggttt actatcagaa atcgaaatta ctgatgcgca
     5881 gaaagactgg attcgacggg cgtttcatcg tatcacagat atgaatcaat tagcggtaca
     5941 ttattatttt catcctcaaa ctggaggaag aacatcgata aaagtgactt taccggctat
     6001 attaaaatct gttcgatcta agcgaacggt agcatggtta aaggaacttg acgtatacaa
     6061 gttggatgaa aatcaagaac tcatcaaccc ttattattta ttaccagaac tcggtttacc
     6121 cggtacagaa ggcagtatta acgatggttc gggagcaatg atagcatatc aatatctcca
     6181 attgagcatt gagaatcgag aatcagaaac ctatattcaa taccgaaatg cgttgcttcg
     6241 ctattgtcag ctagatacct tagccatgtt gattatttat gaagaatggc tattgatgga
     6301 agaaagcaag atgcctagtt aaattaggca tccgctccag ccgaaggatt gggttttgta
     6361 gctctaaaac gatctttgag tttaagaaat ggagattcaa taaatcggta gctgagtgaa
     6421 gaaaagacaa tggtaccgaa taaacaaagt atgatttgga ataaataaac ccaaggacta
     6481 tcgacagctg gttgtaatcc ggaggttttg aataaataga aaatcaacca gatcataact
     6541 aagtgccaga gatataatcc ataggataat tttccccaag aactgagtaa tttgattttt
     6601 ccgaatttga aaaatgattc ttcagaatac tcttgctctc caataataaa tgcaaaaatg
     6661 acagaaaaaa tagctggttc aattgcttgc acccagttca taatcgggta aaactgaaag
     6721 ctatacgtac gatcacgtat gatgattaga acaattgcag cgatataagg aagaacgatt
     6781 gatttacgtg gaagtgtgcg aaaatactga cggagttttt cggaataaaa taatccataa
     6841 gctaataatg cgcccatggt tatatcggac aaaatactta gggtatgaaa acgcgtcacc
     6901 aacatagcgt gattgcgtaa ttcattaaag cgattgacca catcaacagg atgcggtttg
     6961 gaaggatctc ctagactata tgtattaaat aaatgaacga tttcgttcca ctgcataaaa
     7021 cggaaaccgg ctgtaaaaat caatactcca aataaagagt aaatcaaata tttgcgagga
     7081 accagggcaa ttaataaagg ccaaaaaata taaaactgct cttcaacaga aatagaccaa
     7141 agcacagcta aaacaggact tggatatcca tgcatggtta tgtcgaaatt aacaagaaag
     7201 cttagataat acccaattcg atcaatagga ataattgaaa aaacaggtgg aaaaggattt
     7261 gactcaggat gaagttttaa taatccgggt aaaatcacaa aaccaacaaa aactaaaata
     7321 aaatagagtg gccaaatacg taaaaaacgt cttgcgtaaa atgctccaat gttgatttta
     7381 cccgtgtctt cgcgctcttg caacaaaaga ttagtaatta gaaatccact taaagtgaaa
     7441 aaaaaattca cacctaaatc accattttga gcgagtgatt cttctatcca tcgataccaa
     7501 gctgagccat tgtaatacaa accaaaactg gtcatatgta aacaaaacac cgaaaaaaat
     7561 gcaaaaaagc gaagagtatc aagatgttcg aagtatcgta atggtcgcat ggatgctatg
     7621 atttagggct ggaatttagt cagaacagct ggaagcgaat atattatttc tcgtaaagga
     7681 aaattcagta taaaataata aaggagcccg aaagctcctt ttacaattag tgttgaacaa
     7741 ccatacgttt ggtcagtatt cgagatttac tttgtgcttg aattagataa acaccattag
     7801 accatgaagc aacatcaatt tgtgtgaggt tttgatttgc tttaccagtc catattactt
     7861 gtcccgaaac gctataaatc ttaatgcttg gattttcttc taaccatatt tcagaaatca
     7921 acaatgaaat attttcttga gctggatttg gaaataattg aataagcatt gattcaggaa
     7981 tgaaatccaa agaagttgga cctacacaaa acgttatcga atcggaatca tcaaagtttc
     8041 ctcctgttcc aatagaaatt cccaatgtat cataaactgt aaaagatcca tttccactac
     8101 ttccgcagca aattccatcc ccataagaat cgtgcattct caaagaataa catccattag
     8161 ctaaacacaa tggaaaatta gatgtaccaa gggaaactgc tcctattgct tgttgcgtaa
     8221 taatttgatt agtagcaaca ttcacgagtt cccaagatgt ttcttccgga tattcatccg
     8281 aaaccaattc aaaacgatat cctgcgcctg cacgattatt cacattaatg ttcaaactgt
     8341 tattattgct ttgctcatca gttgctccat ttacagaaat aatagttgct gagaaatttt
     8401 gatttgtgcc tatactcaat agctgttcag gaagtactac atctacaact tgattataag
     8461 ctaatgtacc tgtccaatca aaggtttgcg ccgctcctcc attaaaagta taactgatat
     8521 ttatacttgt aagagttgta gaaccagtat ttcgaatttt taaagaagga ataaaagaag
     8581 cagtattaca atattcctga actggagaaa taatgctttg catacgagcg tccaacgaaa
     8641 atgatggaac acttgcttcc ataccactaa ccgttagtga atcactatta tttggatcta
     8701 gccaatcttt taaacgcaca ttagcactag ctccatccca tgaagtaaca aatcgtccga
     8761 aattatcatt cacattatcg ttacagttcg catcaccacc aaataactga cctataataa
     8821 ggtgattttc attgaaaagc ggcgatccag aagatcctgg ctctgttgtt ccatcttccc
     8881 aatcaggaat gttccaacaa tcggcatttc ccca
//
LOCUS       DAOOQW010000078         8814 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_9838_length_8814_cov_6.032081, whole genome shotgun sequence.
ACCESSION   DAOOQW010000078 DAOOQW010000000
VERSION     DAOOQW010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 8814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8814
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9838_length_8814_cov_6.032081"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="PK281_06025"
     CDS             <1..348
                     /locus_tag="PK281_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010249649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HOY48937.1"
                     /translation="EEMYITTEAADIVNKSKAEKKNVISVGTTTMRAIESSVSTEGFL
                     KEYSGWTNKFIFPPYDFRVANALVTNFHMPMSTLLIMVSAFAGHDFLMEAYKEAVKEK
                     YRFFTYGDAMLII"
     gene            353..1024
                     /locus_tag="PK281_06030"
     CDS             353..1024
                     /locus_tag="PK281_06030"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF001186.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HOY48938.1"
                     /translation="MRYHVIIVAGGSGLRMQAAVPKQFLLLRGLPVLMHTINRFHQTL
                     PSAQLIVVLPEDQISFWKQLLVEHHFSVPHQVVAGGETRFHSVKNGLARVDEGIVGVH
                     DGVRPLVSEQTILRCFSAAEKTGAVVPVMPVNDSTRIVLADGSNQALERSALRSIQTP
                     QCFEVEKLKRAFEQAYRSDFTDDASVMEAAGFRIELVDGNEENIKITRPSDLILAENF
                     LNSPV"
     gene            1075..2349
                     /gene="purD"
                     /locus_tag="PK281_06035"
     CDS             1075..2349
                     /gene="purD"
                     /locus_tag="PK281_06035"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HOY48939.1"
                     /translation="MKILLLGSGGREHAFAWKLAQSKRLTTLYISPGNAGTQGLGINV
                     ALNPNDIPAVISFVEEHQIDLVLVGPEEPLVRGIVEAFAQHPNVNHVPVIGPDSVGAQ
                     LEGSKDFSKAFMQRHGIPTARYQSFTPATIEEGKAFLHTFQPPYVLKASGLAAGKGVV
                     IPETLAEAERELEDMLGGKFGAAGNVVVIEEFMKGIECSVFVLSDSEHYLVLPEAKDY
                     KRIGEGDTGLNTGGMGAVSPVPFCIPEFMEKIRTRIIEPTIQGLKQEGIVYKGFIFIG
                     IMNVMGDPMVVEYNCRMGDPETEVVIPRIQNDLVDLLEAVANGTLNTHTIQTSPETAV
                     TVMTVSGGYPGDYSKGKIIHGLGELTGELVFHAGTKQEGSDIVTQGGRVLAFTSLGDS
                     IDSAVKRSYKNINTICYDGIYYRRDIGQDLMS"
     gene            complement(2350..3183)
                     /locus_tag="PK281_06040"
     CDS             complement(2350..3183)
                     /locus_tag="PK281_06040"
                     /inference="COORDINATES: protein motif:HMM:NF017932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent peptidase"
                     /protein_id="HOY48940.1"
                     /translation="MLAAFFVAVILIYFQIRELKTPDEDSPIPFDEEDYSDYVQISYM
                     PSDATLHEFLFGNFAFYHLLNPQEKARFRTRLVRIIKTKWFVGREGLQVTDSKILLIG
                     ATMAQLTMGIERFYFPRFDRIVLFPNQFYSRLFEQDIKGLTVYHNGIVILSWPHYEDG
                     YSDATDKLNLGLHEMAHALYLDYFGHRRMLYGFDDWTLVAMPVFEEMQRNEHHPFLRD
                     YARSNMHEFWAVCVEHFFEAPIEFRQKLPELYKAMCQILKQDQARRLEAFAETRSKQA
                     S"
     gene            complement(3198..4313)
                     /locus_tag="PK281_06045"
     CDS             complement(3198..4313)
                     /locus_tag="PK281_06045"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HOY48941.1"
                     /translation="MKKVVFNNKNNSFSEALNAEVKKYFAENHLKQTGDWRLYLKSFV
                     LIPGALAIYISLLTVQMPAVAALALCALLGLCIASIGFNVMHDSCHGSYSESKRINYI
                     MGLTMNALGANAFIWKIKHNIVHHTYTNIDGLDDDIAKSPVLRHCYSQPYKPLHKYQH
                     IYMFFFYGISSMLWALYTDFDKYFKRSIHNTPINHIPMQEHVLFWVSKVLYIVFYMLI
                     PIMMVGFSKFIIGYLFASAVLGLTLALVFQLAHVIELTEFVDATHEPDVRLTIEDSWA
                     VHQMKTTANFATKNKAISWFVGGLNFQVEHHLFPKISHIHYSALSPIVEKVCKEYGVP
                     YYNMPTMIGAIASHYRTMRKFGQEENPSMANPTFAAA"
     gene            complement(4396..5370)
                     /gene="pfkA"
                     /locus_tag="PK281_06050"
     CDS             complement(4396..5370)
                     /gene="pfkA"
                     /locus_tag="PK281_06050"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015345460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOY48942.1"
                     /translation="MKHIAVLTSGGDAPGMNACIRAVVRTAMFKGIEVTGVVAGYEGL
                     IQGDFRKMDSTSVGNIIQKGGTILKTARSARFRTPEGRAEAMKNIRAQGIEGLVVIGG
                     DGSFQGADILAQEYPEIRVIGIPGTIDNDLYGTDFTIGYDTAINTVVEAVDNIRDTAS
                     SHNRLFFVEVMGRDAGLIALRSGIACGAEAILIPETKTHIDKLITQLERGWKRQKSGH
                     VIIVAEGDDAGGAFKIADIVQKKFDKLETKVCILGHIQRGGNPTCMDRVLATRLGYEA
                     VCGLLDGKNRMMAGQVAGKVNFIPLEKAVKHNIDVNETILEIAEIMSL"
     gene            complement(5428..6906)
                     /locus_tag="PK281_06055"
     CDS             complement(5428..6906)
                     /locus_tag="PK281_06055"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOY48943.1"
                     /translation="MKLKYILQVLVFVVLAGGLFLSSCRKDVLDVSPSSKLGFSTDTV
                     YFDTVFVTMGSVTQRFRVYNSSEDKIKVSNIRLAGGSASPFRLNIDGEATINAHDVEI
                     DGKDSIFIFVEVTIDPNNDQNPFVVMDSVLFETNGNLQNVKLMAYGRNADFFRPTVFP
                     KNGFPAYSVVGCDTTWTNNRPKVIIGYLAVDSACSLTIEAGTQVYLYNNSGIWVYKQG
                     RLVVNGTLQDSVVFQGVRKEDSYKNTPGQWDRIWINQGSTGNVINYAVIKNGNIGIQA
                     EDVISFGEGLPSLLTLSNTRIKNMKAFGLYGVHYSINAWNNVISNCGSYALALINGGT
                     YRFRHNTVANYWGESVRSETAVFLSNYQVIQNVTYVANMDFRFTNGIVYGNLQGDNEL
                     DVDSQPGATFNWKFENTCLKIDNENIDPGDVNHFSNMVLNEDPLFRDVQNRDYRLSES
                     STSRDKGSVAVVNESPAIPTDLLGNNRLGDTAPDCGAMEFTP"
     gene            complement(6957..7298)
                     /locus_tag="PK281_06060"
     CDS             complement(6957..7298)
                     /locus_tag="PK281_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HOY48944.1"
                     /translation="MDEALIYDKHIFICTNQRPDGAPRPCCGETQGLALVAAFKKAIK
                     DKQLPVPIRAQKSGCLDLCEQGPTVVIYPEGVFYGKVQLSDVQEIVDSHLVGGIPVER
                     LRLRRPFNTQS"
     gene            complement(7351..8736)
                     /locus_tag="PK281_06065"
     CDS             complement(7351..8736)
                     /locus_tag="PK281_06065"
                     /inference="COORDINATES: protein motif:HMM:NF025714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4403 family protein"
                     /protein_id="HOY48945.1"
                     /translation="MNRSIFVLVLLIVWTSCKTVKPEMPTYEPSRKVYTRAISTISIP
                     LEIPLKLIENKINAQFKGVIYDDDSYTRPTVDDVKIRVYQNNHIGVTAVGNELKFTIP
                     LKIWAQGRFDPCSFCPEVEKQTIFDVEVYLRSKVEILKNYQFRMSTASDGFEWKSQPR
                     IAIGPINIPIGRLLESVIDEQLQSVSKEIDKNVNGAFDLRDQVEALWKVAQEPLLLDD
                     STQTWLRAEPKALLLAPITGDANSLNIILGLETYLETFTGRKPEPPASNPLPDLRLAG
                     RGGQDFSIQIRSELGFDAATSIAKKQLVGQEFHFKKKTIKVEDIQVYGKGDLAYVRLV
                     LSGSLKGELFLSGIPTYNSETNEFSIAQLDYDIHSRSVLVKAASWLLNGTLQKQLQQQ
                     MHFSFDQEVSSIRDDLTKRLKSTSYKNVFTVKGSLKAFKVRDIFVADDHFDIVLDANG
                     NAKILLESADF"
BASE COUNT         2584 a         1865 c         1788 g         2577 t
ORIGIN      
        1 gaggaaatgt acatcacgac cgaagctgcc gatatcgtga acaaatcgaa agcggagaaa
       61 aagaatgtga tttcggttgg aacaaccacc atgcgtgcga ttgaaagttc tgtatctact
      121 gaaggattct tgaaagaata cagcggctgg acgaataaat tcatttttcc tccatacgat
      181 ttccgcgtag cgaatgcctt ggtgacgaat tttcatatgc caatgagcac cttgttaatc
      241 atggtttcgg cttttgctgg acatgatttt ctaatggaag catacaaaga agccgtaaaa
      301 gagaagtatc gcttctttac ttatggcgat gcgatgttaa ttatctaaca gggtgcgtta
      361 ccacgtaatt atcgtggctg gcggaagtgg tttgcgcatg caagctgctg ttcctaagca
      421 gtttttactg ttaaggggat tacctgtttt gatgcatacg attaatcggt ttcatcaaac
      481 cttacctagt gctcaactaa tagttgtact tcctgaagat caaatttctt tttggaagca
      541 attactagtt gagcatcatt tttctgtgcc acatcaagtt gttgctggcg gagaaacacg
      601 ttttcattcg gtcaaaaacg gtttagcacg tgttgatgaa ggaatcgttg gtgttcacga
      661 tggtgttaga ccgctggtga gtgagcaaac tattttacgt tgtttttctg ctgccgaaaa
      721 aacgggagct gttgttccgg tgatgcctgt gaatgattca acacgtatcg ttttagctga
      781 cgggtcgaac caagcactgg aacgttctgc tttgagaagc attcaaacgc ctcaatgttt
      841 cgaggtagaa aaattgaagc gcgcttttga acaagcctat cgcagtgatt ttactgatga
      901 tgcttcggtg atggaagcgg ctggatttcg gattgaacta gtggatggaa atgaggaaaa
      961 catcaaaatt actcgaccgt cggacctgat tttagccgag aactttctga attctcctgt
     1021 ttaagttttt cgatttacgc ttctttcctc caaaaaaacc ccgaatttgc aggcatgaaa
     1081 attcttctgc ttggatcagg tggtcgcgaa catgcgtttg cttggaaatt ggctcaaagt
     1141 aagcgattaa caacacttta catttctccc ggaaatgccg gcactcaagg tttgggtatt
     1201 aatgtggctt tgaatccgaa cgatattccg gcagttattt cgtttgttga agaacatcag
     1261 attgatcttg tacttgttgg tccagaagaa cctttggtac gaggaattgt agaagccttt
     1321 gcacaacatc ctaacgtgaa tcatgttccg gttattggac ccgattcggt tggtgctcag
     1381 ttagaaggca gcaaggattt ctcgaaagct tttatgcaac gccatggaat tccaacagct
     1441 cgttatcaga gttttacccc agcaactatt gaagaaggca aagcattttt acacacgttt
     1501 cagccacctt atgtgttgaa agcgtctgga ttagcagccg gaaaaggtgt tgttattccg
     1561 gaaactttag cagaagcgga acgcgaatta gaagacatgc ttggtggaaa gtttggcgca
     1621 gccggaaatg ttgtagtgat tgaagagttt atgaaaggca tcgagtgttc ggtgtttgtg
     1681 ctaagcgatt cggaacatta tctcgtttta cctgaagcca aggattacaa acgcattggc
     1741 gaaggtgata ccggactcaa caccggagga atgggcgctg tttcgcctgt gccattttgc
     1801 attcctgagt ttatggagaa aatccgaact cgaattattg agcctaccat ccaaggatta
     1861 aaacaagaag gcatcgttta caaaggcttc atctttatcg gaatcatgaa cgttatgggc
     1921 gacccgatgg tggtggaata caattgccga atgggcgatc ccgaaaccga agtggtcatt
     1981 ccacgaatcc aaaacgacct agtcgattta ttggaagctg ttgccaatgg cactttaaac
     2041 acacatacca ttcaaactag tcccgaaaca gctgtcaccg ttatgacggt ttcgggagga
     2101 tatccgggtg attattcgaa aggaaaaatc attcatggac taggtgaatt aaccggtgaa
     2161 ttagtatttc atgccggcac taagcaagaa ggaagtgata ttgtaacaca aggagggcgc
     2221 gtattagctt ttacctcatt gggcgattca attgactcgg ctgtaaagcg ttcgtacaaa
     2281 aacatcaata ccatttgtta cgacggtatt tactatcgtc gcgatattgg tcaagatttg
     2341 atgtcctaat taagatgctt gtttacttct ggtttcagca aaagcttcta aacgtcttgc
     2401 ttgatcttgc ttcagaatct gacacatcgc cttgtatagc tctgggagtt tctgtctgaa
     2461 ttctattggt gcttcaaaaa agtgttctac acaaacagcc caaaattcat gcatgttaga
     2521 acgagcataa tcacgtaaaa atggatggtg ttcgttacgt tgcatctctt caaaaacagg
     2581 cattgcaaca agtgtccagt cgtcgaatcc gtaaagcatt cgtcggtgcc caaaataatc
     2641 gagataaagc gcatgagcca tctcgtgtaa gccaagattt aacttatcgg ttgcatcgga
     2701 atatccatct tcataatgtg gccaagatag aatcacgatg ccgttgtgat aaacggttaa
     2761 gccttttata tcctgctcaa ataaccgaga gtaaaactga ttgggaaata atacgattcg
     2821 atcaaaacgg gggaaataaa aacgctcgat tcccatggtg agttgagcca tggttgctcc
     2881 aataagtaaa atttttgagt cggtaacttg taagccttca cgaccaacaa accatttcgt
     2941 ttttatgatt cgaacaagcc tcgttctaaa ccgtgctttt tcctgcggat ttaacagatg
     3001 ataaaaggca aaattcccaa atagaaactc atgcaaagta gcatcactgg gcatgtacga
     3061 gatttgaaca taatccgaat agtcttcctc gtcaaaagga atcggactgt cctcatcggg
     3121 agtttttagc tctcgaattt gaaaataaat taaaattact gcgacaaaaa atgcagctaa
     3181 catgctgatc ctacttttta cgcagcggcg aaagtcggat tagccatgct tggattttct
     3241 tcctgtccga atttacgcat ggtgcggtaa tgactagcaa tcgcaccaat catcgttggc
     3301 atgttataat aaggaacgcc gtattctttg caaacctttt ccacaatagg acttaaagcc
     3361 gaatagtgaa tgtggctgat ttttgggaac aagtgatgct ctacttggaa attcaatccg
     3421 cccacaaacc atgaaatagc tttgtttttt gtagcaaaat tggcagtggt tttcatttga
     3481 tgaacagccc aagaatcttc aatagttaat cgaacatccg gctcatgcgt agcatcaaca
     3541 aattcggtaa gttcaataac gtgcgccaat tggaatacaa gagctaaggt caagcctaag
     3601 actgcgcttg caaataaata tccgatgatg aatttggaga aaccaaccat cataatagga
     3661 atgagcatat agaacacgat gtacaaaacc ttagaaaccc aaaacaaaac atgttcttgc
     3721 atgggtatat gattgattgg tgtgttgtgt atgctacgtt tgaagtattt atcgaaatca
     3781 gtataaagcg cccaaagcat cgatgaaatt ccataaaaga aaaacatgta gatatgctga
     3841 tacttatgaa gcggcttata tggttgagaa tagcaatgac gaagcactgg cgatttggca
     3901 atatcatcat ccaaaccatc aatattggta taagtatgat gaacaatgtt gtgcttaatt
     3961 ttccaaataa acgcgtttgc accaagagca ttcatggtta atcccatgat gtaattgatt
     4021 cgtttgcttt cggaataact accatgacag gaatcatgca tcacattaaa tccaatagac
     4081 gcgatacaaa gaccgagtaa tgcacacaaa gcaagtgcag caacagcggg catttgaaca
     4141 gttaaaaggg aaatataaat agctaatgct cctggaatca ggacgaatga ctttaaatac
     4201 aaacgccaat caccggtttg ttttagatgg ttctctgcga aatacttttt tacttcagca
     4261 tttaaagctt cagagaacga attgttcttg ttgttgaata ctactttttt caaagagggt
     4321 tgattttcgt gaaaggtaag tcaaaaagtg acaagagccc ccaattaacc attttgaact
     4381 aacaattgtt tgtgtttaaa gtgacataat ctcagctatt tccaagattg tttcgttcac
     4441 atcgatgtta tgtttaacgg ctttttcgag cggaatgaaa ttcactttcc cagctacttg
     4501 tcccgccatc attcgatttt ttccatctaa taaaccacaa acggcttcat aaccaaggcg
     4561 agttgcaagc acgcgatcca tacaagttgg attacctccg cgttggatat ggccaagtat
     4621 acaaactttg gtttcgagct tatcaaactt cttctgaacg atgtcggcaa tcttaaatgc
     4681 tccaccagca tcatcgcctt cggcaacaat aataacgtgt cccgattttt gacgcttcca
     4741 gccacgttcc agttgggtaa tcaatttatc aatatgcgtt tttgtttcgg gaatcagaat
     4801 cgcttccgca ccacaagcaa ttccgctgcg caaggcaatc aatccggcat cacgccccat
     4861 cacttccaca aaaaacaaac gattatgcga actggctgta tcgcggatat tatcgaccgc
     4921 ttctacaact gtattaatgg cagtgtcgta tccaatggta aaatcggtgc cgtataagtc
     4981 gttatcgatt gttccaggga ttccaatcac acgaatttcg ggatattcct gtgctagaat
     5041 atctgctcct tgaaatgaac catctcctcc aatcaccacc aaaccttcga tgccttgagc
     5101 tcgaatgttt ttcattgctt ctgccctacc ttctggtgtt ctaaaccgag cgcttcgggc
     5161 agtttttaat attgttccac ctttttgaat gatgtttcca accgaagtcg aatccatttt
     5221 tctaaaatcg ccctgaatca agccttcgta accagcaacc actccagtta cttcaatgcc
     5281 tttgaacatc gccgtccgaa caaccgcacg aatacaggcg ttcattcccg gtgcatcacc
     5341 accagaggtc aatactgcaa tatgtttcat taataaagtg ttaatctgac acaaagataa
     5401 agcaacttat cttttatccg aacatgctta aggagtaaat tccatcgccc cacaatcggg
     5461 agcagtatct cctaaacggt tgtttcctag caaatctgtt ggtattgcag gactctcatt
     5521 cacaacagca acacttcctt tatcacgaga tgtggaactt tccgataaac gatagtccct
     5581 gttttgaaca tctcgaaaca aaggatcttc attcaacacc atattggaaa aatgattcac
     5641 gtctcccgga tcgatgtttt cattatcgat tttcagacag gtattttcaa atttccagtt
     5701 aaaagtagct ccaggctgac tgtccacatc caattcgtta tctccttgta gatttccgta
     5761 aacgatccca tttgtaaaac gaaaatccat gttagcaacg taagtgacat tttggattac
     5821 ctgataatta ctcaaaaaga cggcagtttc agagcgaact gattcgcccc agtaattggc
     5881 cacggtattg tgtcggaatc gatatgttcc tccatttatc aaggccagag cataagagcc
     5941 acaattagaa atgacgttgt tccaagcatt gatcgaataa tgaacgccat ataaaccaaa
     6001 ggccttcatg tttttgattc gagtattcga gagtgttagc aaacttggca atccttcgcc
     6061 aaacgaaata acatcctctg cttgaattcc aatgtttcca tttttaatca ccgcatagtt
     6121 tatcacatta ccggtgcttc cttgattaat ccaaattcga tcccactgac cgggtgtgtt
     6181 tttatatgaa tcctccttcc taacgccctg aaaaacaacc gaatcctgca aggttccatt
     6241 cacaaccaaa cgaccttgtt tatacaccca aattccagag ttattgtaca agtatacttg
     6301 agttccggct tctattgtga gcgaacaggc cgaatcaact gctagatatc cgattatgac
     6361 tttagggcga ttgtttgtcc aagttgtatc acagccaaca accgaataag ctggaaaacc
     6421 atttttgggg aaaactgttg ggcgaaagaa atcggcattt ctgccatacg ccatcaattt
     6481 cacattttga aggtttccat tggtttcaaa caacacgcta tccatcacca caaaaggatt
     6541 ctggtcatta ttcggatcaa ttgttacctc tacaaatatg aaaatactgt ctttcccgtc
     6601 gatttccaca tcgtgcgcat tgattgtagc ttctccatca atatttaaac ggaatggtga
     6661 tgcgcttcct ccagctaatc ggatattcga aactttaatt ttgtcttcgc tcgaattgta
     6721 aactcggaat cgttgtgtta ccgagcccat tgttacaaaa acagtatcga agtaaacggt
     6781 atcagttgag aaacccagtt tagatgatgg actaacatct agcacatctt ttctgcaact
     6841 gctcaggaaa agtcctcctg cgagaacaac aaacaccaaa acctgcagaa tgtattttaa
     6901 tttcatagac ttcgccagaa cgccaaaggt aacagattat tttccagagc ttccaatcaa
     6961 gactgtgtat tgaacggacg tcttaacctt aaacgttcca cgggtattcc gccaacaaga
     7021 tgtgaatcaa caatttcctg aacatcactt agttgaacct ttccataaaa tacaccctca
     7081 ggatagatta ccacagttgg tccttgttcg cataaatcga gacagccgga tttttgagct
     7141 cgaataggaa caggcagttg cttgtctttt attgctttct taaatgctgc aaccaaagcc
     7201 aatccttgtg tttcgccgca acatgggcga ggagctccgt cggggcgctg attcgtgcaa
     7261 ataaaaatgt gcttgtcgta aatgagtgct tcgtccatgt ggcaaatgtc gctgttaaag
     7321 tttggttctc ataataggaa aatttctccg ctagaaatcg gcagattcta gaaggatttt
     7381 agcattgcca ttggcatcaa gaacgatatc gaaatggtca tctgccacaa aaatatcacg
     7441 cactttaaat gctttcaaac ttcctttaac cgtaaaaacg ttcttgtaag atgtgctttt
     7501 caaccgctta gttaaatcgt cgcgaatgct gctaacttct tggtcgaacg agaagtgcat
     7561 ttgctgttga agctgttttt gcaacgttcc attaagcaac caactagcgg cttttactaa
     7621 aacactccgt gagtgaatat cataatccaa ctgagcaatg ctgaattcgt tggtttcgct
     7681 gttgtaggtt ggaattcctg ataagaatag ctcacctttc aaacttccag atagcaccaa
     7741 tcgaacatag gccaaatcac cctttccgta aacttgtatg tcctcgactt tgatggtctt
     7801 ctttttaaaa tgaaactctt gtccaaccaa ctgtttcttg gcaatagaag ttgctgcatc
     7861 aaagcctaat tcggaacgta tttgaatact aaaatcctgt ccgcctcgac cagccaaacg
     7921 caagtcaggt aaaggattgc tcgcaggtgg ctcgggttta cgaccggtga aggtttcaag
     7981 gtatgtttca agccctagaa taatattcaa cgaattagca tcacccgtta taggtgcaag
     8041 aagtaaggct ttcggttcgg ctcgcaacca agtttgggtt gaatcgtcga gcaatagtgg
     8101 ttcttgagca actttccata aagcttcaac ctgatcacgc aaatcaaaag ctccgttaac
     8161 atttttgtca atctctttac tcaccgactg taattgctcg tcaatcacgc tttccagcaa
     8221 tcgtccaata ggaatattga ttggtccaat tgcaattcga ggctgacttt tccactcaaa
     8281 accatccgaa gcggtactca ttcgaaactg gtagtttttg aggatttcga cttttgagcg
     8341 gagataaact tccacatcaa aaatggtttg cttctctact tccggacaaa atgagcatgg
     8401 atcaaatctt ccttgtgccc aaattttaag gggaatggtg aattttagct catttccaac
     8461 tgccgtaact ccgatgtgat tgttttgata aacccgtatt ttaacatcat caaccgttgg
     8521 acgcgtatag ctatcatcat cgtaaatcac acctttaaac tgggcattga ttttattttc
     8581 gatgagtttc agcggaattt ctaatggaat gctgattgtt gaaattgcac gcgtataaac
     8641 cttacgggat ggctcatacg tgggcatttc aggttttact gttttacagg aagtccaaac
     8701 aataagcagg acaagcacaa aaatggaacg attcaacagg tagattatca aaactggtcg
     8761 caaaggtatt gctctgttgg cttatcaagg ttcggtcgtt caaaatttaa gtgg
//
LOCUS       DAOOQW010000079         8797 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_9858_length_8797_cov_5.678678, whole genome shotgun sequence.
ACCESSION   DAOOQW010000079 DAOOQW010000000
VERSION     DAOOQW010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 8797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8797
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9858_length_8797_cov_5.678678"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(8..496)
                     /locus_tag="PK281_06070"
     CDS             complement(8..496)
                     /locus_tag="PK281_06070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48946.1"
                     /translation="MKKYSTLLSLFIIILVGLVGCEKFTEDEGIQWEKPRERLRKQLW
                     RISHYQINGIDSSLEKINAIHNNWIKFNWDPEYDWVSYKLYLWSEPQMEIGYWGFQGE
                     NLFHLGTDTFLLGPSFTDTALVNTILLMNGTYEIKRLSDYQFKLQKNPNTYIEFMGGP
                     KK"
     gene            complement(558..1013)
                     /locus_tag="PK281_06075"
     CDS             complement(558..1013)
                     /locus_tag="PK281_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48947.1"
                     /translation="MIYFGACKKYEEDDNLVTFRTPLKRIAGKHEITEFTFNGSDSTS
                     YLKNRFGNFYLLFLDNLNDQGDYYLNVHTKEDNKLIASGRWGFLTKEEQIAFEFKGDS
                     NQVFSSGNLAIKKLTYDEIILEASNAYLNKVDSSLVQTPGYLRFKLIRF"
     gene            complement(1162..1818)
                     /locus_tag="PK281_06080"
     CDS             complement(1162..1818)
                     /locus_tag="PK281_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElyC/SanA/YdcF family protein"
                     /protein_id="HOY48948.1"
                     /translation="MLRRRWFRRTLLFLFLALLLTGGVVYWSNYWIDEESDSWIFTDT
                     QEMPHTKVGLLLGTSKYNRSGNPNLFFRYRIDAAVELYNSHKVDYLLVSGDNRTLTYN
                     EPRDMRRALIARGIPDSVIYLDYAGIRTFDSVIRCYRVFGQTSFTIISQEFHVERALF
                     IARHYGLSAVGFAARSVPNVYSLTTVFREYLARLKAVLDVYILPTKPRFLGDPVQVGK
                     "
     gene            1911..4328
                     /locus_tag="PK281_06085"
     CDS             1911..4328
                     /locus_tag="PK281_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOY48949.1"
                     /translation="MSSPLNESPSSSELSFEEFLASVINDYRIACISRETSLLGRKEV
                     LTGKAKFGIFGDGKEIPQIAMAKAFKNGDFRSGYYRDQTFMLAAGLMTVEAFFAQLYA
                     HADEEAEPMSSGRSMNGHYATTTVNPDGSWRELTSLKNSSADISPTAGQMPRLLGLAL
                     ASKVFRNNPELKNATNLSINGNEVAFGTIGDASTSEGLFFETINAAGVLQVPMAISVW
                     DDGYGISVARKFQTTKSSISKALAGFEANEDGNGFRIYKGKGWDYPGLCELYEKGISR
                     MREEHVPALFHIEEVTQPQGHSTSGSHERYKTKERLDWEAEFDCIKKMREWMLETALS
                     TTEELDAIEKEAKTFVRDARRAAWDAFQAPILKTKTDVLALLESLLAEHSNSEVASLR
                     DQLLANKEPLRRDILEAVRKSLRITRSEKSASRQALKNWLNQEQTANFDRYSSWLYTE
                     NTGSALNIEAVAAEYSEEEKLVDGREILRDNFDAILANNPYFLTFGEDSGGIGGVNQT
                     LEGLQKKYGEIRVSDTGIREATILGQGIGMAMRGLRPVAEIQYLDYLLYAIQIMSDDL
                     ATLRYRTKGKQKAPLIVATRGHRLEGIWHSGSPMGMIINAVRGMYVCVPRNMTQAAGF
                     YNTLLAADDPALIIEPLNGYRTKERQPENMGEFRVPLGIPEVLQEGNDVTLVTYGSCC
                     KIASDACKELANLGVSVELIDVQTLLPFDRHHRIAESLKKTGKLFILDEDVQGGASGF
                     MLQQILEVQKGFRYLDAEPVTLTSKDHRPAYGSDGDYFSKPAVDDVVEKVYAVLHEYA
                     PTRFPEI"
     gene            4328..5137
                     /gene="murQ"
                     /locus_tag="PK281_06090"
     CDS             4328..5137
                     /gene="murQ"
                     /locus_tag="PK281_06090"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HOY48950.1"
                     /translation="MERTTEADSRYDGLEHMSTADLLSNINREDHTVATAVQKEIPRI
                     ERLVDAVVEKMKLGGRLFYIGAGTSGRLGVVDASECPPTFGIEFDRVIGIIAGGDTAI
                     RKAVEFAEDDLDQAWKDLNEWKINSNDVVVGIAASGTTPYVIGGVSDAKKAGCITGCI
                     TCNAGSPLAGLVDFPIEAVSGPEFVTGSTRMKAGTAQKLVLNMLSTAVMIRLGRVKGN
                     KMVDMQLTNNKLVDRGTRMIMKAVGKSEEESRSLLLQHGSVRKAIDAVLQS"
     gene            5146..5715
                     /locus_tag="PK281_06095"
     CDS             5146..5715
                     /locus_tag="PK281_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48951.1"
                     /translation="MQELEPFYNWRDYYISADDPASPFYGREYSEFEYSSTIYNFYIH
                     PQWDEFGSETLYLKVLFADYDRGFAVIEFIGEWNDAIGNDVMFLKRDIIDLMISEGIT
                     NFILIGENVLNFHAESEDYYEEWFDDIEDGWIVGLNFREHVVREFNDYGIDQYILFSG
                     RFDEINWRKYQPVALFELIQSYMQRRLNP"
     gene            5791..6681
                     /locus_tag="PK281_06100"
     CDS             5791..6681
                     /locus_tag="PK281_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48952.1"
                     /translation="MKKSLLTILLFLPLFQYAQVDVFGGGAITMGIKSNHVSYSGFYG
                     RTLNNGNSPGAILPNQDLELITDQGSALNIDFHYYKFDDSHFTVYDMSGMVYFLSYFG
                     NMGSNHSGETGYALANWATTPSENLLKVPNPVDFTKYSRFWNFDLLSIKASFGRKNIN
                     GGFNMVFKEMGVTGPFTYFVDKNNQYVYHNLTNVHSLRILIGPNIAFRKTIGMFSVVS
                     MAGVNFSANREDFHIKYNPFFSTVLFFGKGLGGTLGFKYEVVKGYSERQYTGDEILIN
                     PVSLNQFEIKLGLNLTGTKK"
     gene            complement(6701..7165)
                     /locus_tag="PK281_06105"
     CDS             complement(6701..7165)
                     /locus_tag="PK281_06105"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOY48953.1"
                     /translation="MQTFQLPFNVRVYGLVINSRNEILLSDEYCQSQFMTKFPGGGLE
                     YGEGLIDGLKREFQEECNEEIEIIRHFYTTDFFQQSMFRGGGQLISVYYLCRFMNEPQ
                     FPTVQNPYEGVAEINDSQAFRWISIDEINSDHVTWPIDKKVVEIVLMEFNQK"
     gene            complement(7250..8605)
                     /locus_tag="PK281_06110"
     CDS             complement(7250..8605)
                     /locus_tag="PK281_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008257420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHYH protein"
                     /protein_id="HOY48954.1"
                     /translation="MKKISILICLILLAGIGQAQTNPAITSWLQNTTTTGSYYVAGNP
                     TPISNSILVNCQSVQYSTNWVYVTATGIPSYVTGPFQDGNPSQATNQNAIFKFPLNPT
                     QNMGTPTATTGGNIGVFINGVALFDYRDGVSWNNNTQAEGGGPIQGPPGQGIWNRDAI
                     VAERAGFDCAKGHPAMGNYHHHQNPSAFSLDVNVVSTVCNLYDADGLYVLDSMQHSPL
                     VGFAYDGFPIYGGYGFKNTDGTGGITRMKSSFQLRNITTRTTYANGTDVTDGPVVSTT
                     YPLGHYREDYEFVAHTESDYLDIHNGRFCVTPEYPEGIYAYFCTVDEDWNSAYPYAVG
                     PTFYGNKVAAKVTTIGEAVTTYTPTVLSNSSALSNDASIHVFPNPSSDFIAVQIGDLV
                     HDDVVVRLFDLTGKLISQSVVFKGSTATFIDTRTLYSGQYLVQLNDGRETITRKLIIN
                     H"
BASE COUNT         2730 a         1691 c         1794 g         2582 t
ORIGIN      
        1 tattgaatta ttttttagga ccacccatga attcaatgta agtattagga ttcttctgaa
       61 gtttaaattg ataatcagat agccttttga tttcataagt tccattcatt aataaaattg
      121 tattaactaa tgcagtatca gtaaaagatg gacctaatag aaatgtatct gtacctaaat
      181 gaaaaaggtt ttcgccttga aatccccagt atccgatttc catttgaggt tctgaccata
      241 aataaagttt ataacttacc caatcgtatt caggatccca attaaattta atccaattgt
      301 tatggatagc attaattttt tccagggaag aatcaatacc atttatttga taatgggaaa
      361 tacgccacaa ttgtttacgt aagcgttctc ttggtttttc ccattgaata ccttcatcct
      421 cagtaaattt ttcacaacca actaatccca caagaattat aataaataga gataataacg
      481 tcgagtattt tttcattttt ttattctaga atgaatagat atcactttac ttacttgatt
      541 aatgagaatt aataaaatca aaacctaatc aacttaaatc tcaaatatcc aggtgtttga
      601 actaaggaag aatctacctt gttaaggtaa gcatttgatg cttctaaaat gatttcatca
      661 tacgttaatt tcttaatagc cagatttcca cttgagaata cttggtttga atctccttta
      721 aattcaaaag caatttgctc ctctttagta agaaatcccc agcgaccact tgcaattagc
      781 ttattatctt cttttgtatg aacatttaaa taataatcac cttggtcgtt caaattatct
      841 aaaaacaaca aataaaaatt tccaaatctg ttttttaaat acgaagtaga atcgcttcca
      901 ttaaatgtaa attcggtgat ctcatgcttg cctgcaattc tctttaaagg cgttcgaaag
      961 gtcactaaat tatcgtcttc ttcgtatttt ttgcaagccc caaaatatat cattactagg
     1021 aatgcgaata gaaacttgaa cctcattttc gtagccttta tcaatattta actcaaattt
     1081 attcactctt ctgaagataa acaacacata aaatcaccaa ttcgattgat ttcacgtaaa
     1141 tgagctaaaa atccaaatct cttattttcc tacctgaacc ggatcaccta aaaatcgagg
     1201 cttggttggc aaaatgtaca catcgagcac tgcctttaat cgggctaaat attcgcgaaa
     1261 tacggttgtt aacgaataca catttggaac acttcgggcg gcaaatccaa ctgcacttaa
     1321 accataatgt cgagcgataa ataaggcgcg ctccacatga aattcttgcg aaataatggt
     1381 aaacgatgtt tgtccaaata cccgataaca acgaatcacc gaatcgaacg ttcgaatgcc
     1441 agcataatct aaatagataa ccgaatcggg aattccacgg gcgatcaaag cccttcgcat
     1501 atcgcgtggt tcattgtaag tcaaggttcg attatcaccg ctcacgagga gataatcaac
     1561 tttgtggctg ttatataatt ctaccgcggc atcaatacga taacgaaaaa acaaattagg
     1621 attccccgaa cggttgtatt tactcgtgcc taacaataaa cctactttgg tatgcggcat
     1681 ttcctgcgtg tcggtaaaaa tccaagaatc actttcttca tcaatccagt aattactcca
     1741 atacacaact cctcccgtta gcaaaagcgc taaaaagaga aacagaagtg tacggcgaaa
     1801 ccaacggcga cggagcattt ttcaaatatc ggaaattctc cccgattctc cgatagtggt
     1861 ctgttcatca gccaagccga attgttatct ttgccgcaaa ttccgcctgt atgagcagtc
     1921 ctctaaacga atccccttct tccagcgaac tgagtttcga agaattcctc gcatccgtta
     1981 ttaacgatta ccggattgcc tgcatcagcc gcgaaacaag ccttctcggg cggaaagaag
     2041 tccttaccgg taaagccaaa ttcggtattt ttggcgatgg gaaagaaatc cctcagattg
     2101 ccatggcgaa agccttcaaa aacggcgatt tccgttcagg ttactaccgc gatcaaacgt
     2161 tcatgcttgc agccggactc atgactgttg aagcattctt tgcccaacta tacgcccatg
     2221 ccgacgaaga agcagaacca atgtcgtcgg gacgttccat gaatggacac tatgcaacca
     2281 caacagtcaa tcccgatggt tcgtggagag aattaactag cttaaaaaat tcatcagccg
     2341 atatatcacc tactgccgga caaatgccgc gattattagg tttagcattg gcttccaagg
     2401 tttttcgcaa caatccagaa ttgaaaaatg cgaccaactt gtcgataaac ggtaatgaag
     2461 tagcctttgg aaccattggc gatgccagta cttccgaagg attatttttc gaaaccatta
     2521 atgctgctgg agttttacaa gttccaatgg cgatttcggt gtgggatgat ggttatggaa
     2581 tttcggtagc acgtaaattt caaaccacta aatcgagcat ttcgaaagca ttagccggat
     2641 ttgaagcgaa tgaagatggt aacggattcc gcatctacaa aggaaaaggt tgggattatc
     2701 caggactttg cgagctttac gaaaaaggga tcagccgcat gcgtgaagaa catgtgccgg
     2761 ctttgtttca catcgaagaa gtaactcaac cacaaggaca ttctacttct ggctcacacg
     2821 aaagatataa aaccaaagag cgtctcgatt gggaagccga attcgattgc atcaagaaaa
     2881 tgcgcgaatg gatgctcgaa acagcattat caaccaccga agaattagat gcaatagaga
     2941 aagaagcaaa aacgtttgta cgtgatgcgc gtcgtgctgc ttgggatgca tttcaagctc
     3001 cgattttaaa aacgaaaacg gatgtacttg ctttgttgga atccttgtta gcagagcatt
     3061 ccaacagcga agttgcatct ttacgtgatc aacttttggc gaataaagaa ccacttcgtc
     3121 gcgatatttt agaagccgtt cgcaaatcct tgcgaatcac ccgttcggag aaatccgctt
     3181 cgcgtcaagc attaaaaaat tggcttaacc aagagcaaac agctaatttc gatcgttaca
     3241 gttcatggct ttacaccgaa aacacaggtt cagccttgaa tattgaagct gttgctgctg
     3301 aatattcgga agaagaaaaa ttagtcgacg gacgagaaat cttgcgagat aatttcgatg
     3361 ccatccttgc caacaacccg tatttcctca cgtttggaga agatagcggt ggaattggtg
     3421 gagttaatca aacactcgaa ggattacaga aaaaatacgg tgagattcgg gtgagtgata
     3481 ccggaatccg cgaagctact atactcgggc aaggcattgg aatggccatg cgtggattac
     3541 gtcccgtggc ggagattcaa tacctcgatt atttgctgta cgccattcag attatgagtg
     3601 atgatttggc aacactgcgc taccgtacta aaggcaagca aaaagcacca cttattgttg
     3661 caacgcgtgg acatcgttta gaaggaatct ggcattcagg ttcgcctatg ggcatgatta
     3721 tcaacgccgt tcgaggcatg tatgtatgtg ttcctcgcaa tatgactcag gctgcaggat
     3781 tttacaatac gcttttagct gctgatgatc cagctttaat tattgaaccg ttgaatgggt
     3841 atcgtaccaa agaacgtcaa cccgaaaaca tgggagaatt ccgcgttcca cttgggattc
     3901 cggaagtgtt gcaagaagga aatgatgtta cgttggtgac ctatggttca tgctgcaaaa
     3961 ttgcttcaga tgcctgcaaa gaattagcaa atttgggtgt ttcggtagaa ttgattgatg
     4021 tacaaacctt gttgcctttc gaccgtcatc accgcattgc cgaaagtttg aaaaagacag
     4081 ggaaattgtt cattctcgac gaagatgtac aaggtggagc aagtggattt atgcttcaac
     4141 agatactcga agtgcagaaa ggtttccgct acttagatgc tgaaccggta acgctaacgt
     4201 cgaaagatca tcgtccggct tatggttccg atggagatta cttctctaaa ccagcagtgg
     4261 atgatgttgt tgaaaaagtg tacgcggttt tacacgaata tgcccctacc cgattccctg
     4321 aaatctaatg gaaagaacca cagaagccga ctcacgctat gatgggcttg agcacatgag
     4381 tactgccgat ttactcagta acatcaaccg agaagatcat acagtagcca ctgcggtaca
     4441 aaaggaaatt ccacgcattg aacgattggt agatgccgtt gtggagaaaa tgaaactagg
     4501 tggtcgcttg ttttacattg gtgctggcac aagtggacga ttaggtgtag tggatgcatc
     4561 ggaatgtccg cctacgtttg ggattgagtt tgatcgggtg atcggcatca ttgcaggtgg
     4621 cgatacagcc attcgtaaag cagttgaatt tgctgaagac gatttagatc aagcttggaa
     4681 agatttgaat gagtggaaaa tcaactcaaa cgatgtagtt gtgggcattg cagcttcagg
     4741 aacgacaccg tatgtgattg gtggagtttc ggatgcgaaa aaagcaggat gcatcacagg
     4801 ttgtattacg tgcaatgctg gaagtccatt agctggattg gttgattttc cgattgaagc
     4861 ggtaagcgga ccagaattcg ttaccggaag cacccgtatg aaagcaggca ccgcgcaaaa
     4921 gctggtgttg aacatgttga gtaccgcggt aatgatacga ctcggtcgag taaagggcaa
     4981 taagatggtg gatatgcagc ttacgaataa caaattggtc gatcgaggca cgcgcatgat
     5041 tatgaaagct gtaggaaaat cggaagaaga atctcggtct ttgttgttgc agcatggcag
     5101 tgtacgcaaa gcgattgatg cggttttgca atcctgattc gtagtatgca ggaactcgaa
     5161 ccattctata actggcgcga ctattacatt tcggccgatg atccggcttc gccattttat
     5221 ggaagagaat acagcgagtt tgaatacagc agcacgattt ataatttcta tatccatccg
     5281 caatgggatg aatttggctc cgaaacgctt tatttaaaag tactttttgc cgattacgac
     5341 cgcggttttg ccgtgattga atttattggt gaatggaatg atgccattgg taacgatgtg
     5401 atgtttctca aacgagatat cattgattta atgatttcgg aaggcatcac gaattttatt
     5461 ttaattggtg aaaacgtatt gaatttccac gccgaaagtg aggactatta cgaagaatgg
     5521 ttcgatgata ttgaagatgg ctggatagta ggattgaatt tccgggaaca cgttgttcga
     5581 gaattcaatg attatggaat tgatcagtat attttattca gcggtagatt tgatgaaatc
     5641 aactggcgca aatatcagcc agttgcactg tttgagctta ttcaaagcta tatgcagcgt
     5701 aggttgaatc cttagtgaaa tttctatttg aaacataata gaagtacgtt cgtaatcaca
     5761 tttgattttt tttcaaattc taccaaaaac atgaaaaagt ctctcttaac tatactacta
     5821 tttttaccat tattccaata tgctcaagtg gatgtgtttg gtggaggagc aattacaatg
     5881 ggaataaaat cgaatcatgt ttcgtattcg gggttttatg gtaggacatt aaataatgga
     5941 aactcaccag gagctattct tccaaaccaa gacctagaac ttattactga tcaaggctct
     6001 gctttaaata ttgatttcca ttattacaaa tttgatgata gtcatttcac tgtatatgat
     6061 atgagtggga tggtttattt cttaagttac tttggaaata tgggctcaaa ccatagtgga
     6121 gaaacgggtt acgctttagc taattgggca accacacctt cagaaaattt attaaaagtc
     6181 cccaatccag ttgattttac taaatattcc cgtttttgga attttgatct tttaagcatt
     6241 aaagcatctt ttggaagaaa gaatattaat ggtggattta atatggtatt caaagaaatg
     6301 ggagtgactg gtccattcac ctattttgta gataaaaaca atcagtatgt atatcacaat
     6361 ttaaccaatg tgcacagctt gcgaatatta atagggccca atattgcttt tagaaaaaca
     6421 attggaatgt tctcggtagt atcgatggct ggtgtaaatt tcagtgcaaa tcgtgaagat
     6481 tttcatatta aatacaatcc attttttagt acggttttat tcttcggaaa aggcctaggt
     6541 ggtacgctag gattcaaata cgaagtagtt aaaggatatt ctgaacgtca atatactggt
     6601 gatgaaatat tgataaatcc agtaagtctg aatcaattcg aaattaaatt gggccttaat
     6661 ttaacgggga ctaaaaaata attccgattt agtatccttt ttatttctga ttgaattcca
     6721 ttaatactat ttctaccact ttcttgtcaa ttggccaagt cacgtgatct gaatttattt
     6781 catcaatcga gatccaacga aacgcttgtg aatcgttaat ttctgcaaca ccttcgtaag
     6841 gattttgaac cgttggaaat tgtggttcat tcataaaacg acagaggtaa tacacactaa
     6901 ttaattgtcc gccgccgcga aacatcgatt gctgaaaaaa atcggtggtg taaaaatgtc
     6961 ggatgatttc aatttcctcg ttgcattctt cctgaaactc gcgctttagt ccgtcaatta
     7021 agccttcgcc gtattcgagt ccgccaccgg gaaacttagt catgaactga ctttgacaat
     7081 attcatcgct cagcaaaatt tcgtttctgg aattgatgac taaaccataa acccgcacat
     7141 taaaaggaag ctgaaacgtt tgcatggtca aatatacact tgaatgaggc aggaaataaa
     7201 aaaggctgca atctttcgaa aacagccttt aaacattgaa acctaaagct taatggttaa
     7261 tgatcaattt acgggtgatg gtttcacgac cgtcgtttaa ttgaaccaaa tattgtccgg
     7321 aatataaggt gcgcgtatcg ataaatgtgg ctgtactacc tttaaacacg actgattgag
     7381 aaatcaattt tcccgttaaa tcgaacaagc gcactactac atcatcatga actaaatctc
     7441 caatttgaac agcaataaaa tcgctgcttg gatttggaaa aacatgaatc gaggcatcat
     7501 tcgaaagtgc acttgaattg cttagtacag ttggtgtgta agtcgttaca gcttctccaa
     7561 tagttgtgac ttttgcggct actttatttc cgtaaaatgt tggaccaacg gcataaggat
     7621 aagccgaatt ccaatcttca tcaaccgtgc agaaataggc gtaaatacct tctggatatt
     7681 cgggtgttac gcaaaaacga ccgttatgaa tatcgaggta atcggattct gtatgagcaa
     7741 caaattcgta gtcttcgcga taatggccta aaggataagt agtgcttaca acaggaccat
     7801 cagtcacatc tgtaccatta gcataagtag ttcgagtggt gatgttacgt aattgaaaac
     7861 ttgatttcat gcgagtaatt ccacctgttc catcggtatt cttaaaacca tatccaccat
     7921 aaataggaaa accatcgtaa gcaaaaccaa caagtggaga atgttgcata ctatctaaca
     7981 cgtacaatcc atccgcgtcg tataaattac acacagttga aaccacatta acatctaaac
     8041 taaaagctga aggattttgg tgatgatgat aattccccat agccggatga ccttttgcgc
     8101 aatcgaaacc agcacgttcg gcaacaatag catcacgatt ccaaattcct tgtcctggag
     8161 gaccttgaat gggaccacca ccttctgctt gtgtattatt gttccaacta accccatctc
     8221 ggtaatcgaa taaggcaaca ccgtttatga aaacaccaat atttccgcca gttgtagctg
     8281 ttggtgttcc catattttgt gtaggattta acggaaattt gaagattgca ttttgatttg
     8341 ttgcttgtga tggatttcca tcttgaaacg gacctgtcac atagctagga atgcctgttg
     8401 ctgtcacata aacccaattt gttgagtatt gtaccgactg acaattcacc aaaattgaat
     8461 tactgatcgg agttggattt cctgcaacat aataactgcc ggttgttgtt gtattctgta
     8521 accaagaagt gatggctgga ttcgtttgag cctgcccaat tccagcgagg agaatcaaac
     8581 aaatcaaaat ggaaattttc ttcatagaat gaattttagg ttgggcaatt gaatgctatt
     8641 tattggattg aaagacagta tgtgggtaag cgaattcctt attaaaaata aaattccgat
     8701 cactcagcgt taataggaaa gcgattagat caattttttc ctcagagttc aattctatct
     8761 tttggtttaa ttcggttgaa agagttttac ttttttg
//
LOCUS       DAOOQW010000080         8718 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_9957_length_8718_cov_5.688676, whole genome shotgun sequence.
ACCESSION   DAOOQW010000080 DAOOQW010000000
VERSION     DAOOQW010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 8718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8718
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9957_length_8718_cov_5.688676"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..983)
                     /gene="rodA"
                     /locus_tag="PK281_06115"
     CDS             complement(<1..983)
                     /gene="rodA"
                     /locus_tag="PK281_06115"
                     /inference="COORDINATES: protein motif:HMM:NF037961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HOY48955.1"
                     /translation="MANRNKDAVLQNIDWMLVSLYLILVVMGWFNIYAAVYNEEYKSI
                     FDTSQSYGMQLVWIVTSLFIAFVILVIDGEFFPKFAWVLYGAFILLLIVVIFAGKEVK
                     GSKSWIELGAFRIQPAEFAKFATNLALAKYLSNINIKFEQLSTKLIAAAIFAVPALII
                     IAQNETGSALVYGSFILVMYREGLSGNILIMGVAMVILFIAALLMQEWILLTILGVIT
                     AGAFFFVRFKMRNLIIAAGLFVAAAAFVHSVDYTFTNILEPHQKKRINVLLGKEVDPK
                     GAGYNVNQSMIAIGSGGIIGKGYLNGTQTKYNYVPEQSTDFIFCTVGEEWG"
     gene            complement(987..2819)
                     /gene="mrdA"
                     /locus_tag="PK281_06120"
     CDS             complement(987..2819)
                     /gene="mrdA"
                     /locus_tag="PK281_06120"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014200471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOY48956.1"
                     /translation="MNLFSDRKYVISAIFITLVAIFLIRLFYIQVVDDRYKLAANDNA
                     LRHKIEYPERGVIYDRNGKILVYNEAAYDLMVIPKRVKAIDTLDLCKLLDITKEDFTK
                     KLIELKGKKFSSFRPEIFESELSLETYATLQEKLYKFPGFFVQARTLRKYPLRTAAHA
                     LGYVGEVDESMIKDNPYYRQGDNMGLSGIEKSYEKPLRGQRGVKIVMVDVYNREKGSF
                     ENGKYDTASVLGRSLTSSIDAELQQYGEKLMVNKVGSIVAIEPSTGEILTMISSPTYD
                     PNLLVGRVKSKNYRKMLVDTLKPLYNRATMAKYPPGSTFKLVNALIGQHEGVINGTTL
                     FSCSRGFHFGGLTVGCHPHASPVNLLFSITTSCNSYYCNTFKNILQKYPKTQLGFTVW
                     RNHVLSFGLGQKFPVDLPQESKGNVPTVKYYDKYHGAGRWNAITVISLAIGQGEMGVT
                     PLQMANYCATIANRGWFYTPHVIKKVEGGAIDTLYKRKRYTTVDRKYFDPVVEGMRNV
                     VMNGTGRISQLDSLTICGKTGTAQNPHGKDHSIYICFAPMDNPKIAIAVYVENAGFGA
                     TWAAPIASLMLEKYLRREIKRPEMEKRITDANLLPRKEDWISKK"
     gene            complement(2816..3349)
                     /locus_tag="PK281_06125"
     CDS             complement(2816..3349)
                     /locus_tag="PK281_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HOY48957.1"
                     /translation="MIGEIITQLIRFILLIFVQVLVLNQIEISGELNGFIIPNLYVLF
                     LLMLPVNINKILLLFICFLTGLTVDIFGGTAGMHASACLVLGFFRPGFLHLIAPREGY
                     ETTLKLNMAGMGGSTFLIYASTLILIHHLVLFLVEAFTFLNFFELLLRVITCSISTLL
                     LTVLSQFLTQRNREVAK"
     gene            complement(3346..4176)
                     /gene="mreC"
                     /locus_tag="PK281_06130"
     CDS             complement(3346..4176)
                     /gene="mreC"
                     /locus_tag="PK281_06130"
                     /inference="COORDINATES: protein motif:HMM:NF010532.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HOY48958.1"
                     /translation="MRNFFVLIRKYNFFIVFLLLEVVSGVLLVQNSTYQRAAIVGTSN
                     ELVGQMYAAYSNVTDYLILGETNRVLAAENARLRKADSSSFYDPKFEIMKVNDSLGLQ
                     QYEYISARVINNTVTRVNNYITLDKGSLQGVKPDDAVIGSTGVVGIVKDVSEHFSTVM
                     SVLNSNYKVSSRIKKNDYCGSTVWDGDSPQFAQLFDIPSHAQVTVGDTIVTSAFSGIY
                     PQGVMVGRVSEIGTSGESFKEIKLRLSTDFQKLSYVYVVRNLMKAERDTLEAKTLDVK
                     "
     gene            complement(4206..5228)
                     /locus_tag="PK281_06135"
     CDS             complement(4206..5228)
                     /locus_tag="PK281_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016170085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOY48959.1"
                     /translation="MGLFNFFTQEIAIDLGTANTIIIHNDKVVVDEPSIVAIERSTGK
                     VIAVGKQAQQMHGKTHENIKTIRPLKDGVIADFNAAEHMIRGMIKMINPGRKIFSPSL
                     VMVICIPSGITEVEKRAVRDSAEHAGAKEVYLIHEPMAAAIGIGIDVEEPMGNMIIDI
                     GGGTSEIAVIALGGIVCDKSIRVAGDDFTADIEDYMRRQHNIHIGERTAERIKIEVGA
                     ALTEIENPPADYAVHGRDMMTGIPKEIMVSYQEIAHCLDKSIAKIETAILNALEMTPP
                     ELSADIYRTGIYLAGGGSMLRGLDKRISARTKLPVYIAEDPLRAVARGTGIALKNRDK
                     FKFLIR"
     gene            complement(5244..>5570)
                     /locus_tag="PK281_06140"
     CDS             complement(5244..>5570)
                     /locus_tag="PK281_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015481964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase PurH"
                     /protein_id="HOY48960.1"
                     /translation="ILVKHTKSNAIVLVRDLQLLASGTGQTSRVDALKQSVEKARRMG
                     FQTEGSVLSSDAFFPFSDNVELAAEAGIAAIAQPGGSVRDQETIDTANRLNMAMVFTG
                     IRHFKH"
     assembly_gap    5571..5670
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5671..5978)
                     /locus_tag="PK281_06145"
     CDS             complement(<5671..5978)
                     /locus_tag="PK281_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013549718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase PurH"
                     /protein_id="HOY48961.1"
                     /translation="MSQPKRIQSALISVFYKDGLAPLALKLKALGVTIYSTGGTRAFL
                     EDLGIEVIAVEDLTSYPSILGGRVKTLHPKVFGGILSRRDEQSDIAQLEEFAIPEIDL
                     V"
     gene            6266..7510
                     /locus_tag="PK281_06150"
     CDS             6266..7510
                     /locus_tag="PK281_06150"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOY48962.1"
                     /translation="MLFLRLFKESFQFALDSLISNKLRTFLSLLGITIGIFAIISVFT
                     MVDSIKNNLDKSIASLGNNVIFIQKWPWEFSSDYKWWDYMNRPVTQYRELDLVRERSH
                     LAAASAFLIQANVTLEYKSASAENIQISAVSDEYERIKSFEIQEGRYFSDAEIQAGRN
                     KIVVGAEVATQLFGNISPIGKEILVRGDRVEIIGVFKREGQNILDFGLDNAAMVPLNF
                     ARALVDIRSDNVNPYIAVKALDGISNEALIDELTGIMRGIRRIKPREANNFALNETKL
                     ISNGLGSLIGVVNVAGGVIGLFSILVGGFGIANIMFVSVKERTNIIGIQKALGAKNYF
                     ILFQFLFESMLLSIAGGIIGLIIIFLLTLALSAGLDMDISLSLKNISLGIMISSIIGL
                     IAGIAPALSASRLDPVEAMRSK"
     gene            7585..>8718
                     /locus_tag="PK281_06155"
     CDS             7585..>8718
                     /locus_tag="PK281_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HOY48963.1"
                     /translation="MSDKLIRRSFLARLSAGLSTVFMAPLAAKSESVSHAFTSNNPEV
                     KPIKSIKPLGFQWETADPFLFCVHHEDAFPKGNEVMGPDQQYFQGRHLGDDFIIKDGF
                     RMYHGKKVPGFPGHPHRGFETITVVRKGFVDHADSMGAAGRYGDGDVQWMTAGKGVQH
                     SEMFPLLKKDSANPMELFQIWLNLPKRNKMVEPHFKMLWQEAIPIYSHSDRKQLNTRI
                     EVIAGTVGDKKALAPPPNSWAADPKNEVAVWNIKMQAGATWTMPKASAGINRTLYVYE
                     GSSITLAGELVKGYSAAEVQAEMDLLIQAGAEEVSILVLQGRPIGETVIQYGPFVMNT
                     KEEINQAFEDYHRTLFGGWPWPSSDQVHQRSLGRFARHADGKEE"
BASE COUNT         2543 a         1816 c         1764 g         2495 t
ORIGIN      
        1 aatccccatt cttcacccac ggtacagaag ataaagtcgg tgctttgttc aggaacatag
       61 ttgtatttgg tttgcgttcc gttgaggtaa cctttcccaa ttattcctcc cgaaccaatg
      121 gcaatcatcg attgattcac attataaccg gcacctttag gatcgacttc tttacctaaa
      181 agcacgttaa tacgtttctt ctggtgaggc tcgagaatat tcgtgaaggt ataatcgacc
      241 gagtggacaa aagctgcggc ggcaacaaac aaaccagcag cgataatcaa attgcgcatt
      301 ttaaagcgca caaagaagaa agctccagcg gtaattactc ctaaaattgt gagtagaatc
      361 cactcttgca tgagtagtgc tgcgatgaaa agtatcacca tagcaacgcc cataatgagg
      421 atgtttccac ttaaaccttc gcgatacata acaaggataa acgatccata cacgagagca
      481 gatccagttt cgttttgagc aatgataata agggctggaa cagcaaaaat ggctgctgcg
      541 atgagtttgg tgctgagttg ttcaaactta atgttgatgt tactcaggta tttcgccagg
      601 gctagattgg tagcgaactt ggcaaattca gccggttgaa ttcgaaatgc tcctaactca
      661 atccacgatt tagaaccttt tacttcctta ccggcaaaga ttaccacgat tagcagtaag
      721 atgaaagcac catataaaac ccatgcaaac ttggggaaga actcaccatc aatcaccaaa
      781 atcacaaaag ctataaacaa ggatgtaacg atccaaacca attgcattcc gtaactctga
      841 gaagtatcga aaatgctttt gtattcttcg ttgtataccg ctgcataaat attaaaccag
      901 cccatcacca ccaaaatcag gtatagtgat accagcatcc agtcgatgtt ctgtaataca
      961 gcgtctttat tccggttagc catgtcttat tttttggata tccagtcttc tttccgtgga
     1021 agtagattgg catcggtaat tcgtttctcc atttcgggac gcttaatttc ccgacgtaaa
     1081 tatttctcta acatcaaact tgcaatcggc gctgcccaag tagcaccaaa tccagcattt
     1141 tccacataaa cggcaatggc aattttggga ttatccatcg gggcaaaaca aatataaatc
     1201 gagtgatcct taccatgtgg attctgagct gttcctgttt ttccgcagat agttaatgaa
     1261 tctaattggg aaatccgtcc agtaccattc atgaccacat tccgcattcc ttcaaccaca
     1321 ggatcaaaat actttcgatc tacagttgtg tatcgtttgc gtttatacaa agtatcaatt
     1381 gcaccacctt ccactttctt gataacatgt ggtgtgtaaa accaaccgcg gttcgcaatg
     1441 gtagcacagt agttcgccat ttgaagcggt gtaacgccca tctcaccttg tccaattgcc
     1501 aatgaaataa ctgtaattgc attccagcga cctgctccat gatacttatc gtagtatttc
     1561 actgtaggca catttccttt tgattcttga ggtaaatcaa ccgggaattt ctgccccaat
     1621 ccaaaactca acacgtggtt acgccatacc gtaaagccca attgagtctt tggatatttc
     1681 tgcaaaatgt ttttaaacgt gttacagtaa tacgaattac acgatgtggt gatggagaat
     1741 aacaaattta ctggtgaagc atgcgggtga caaccaaccg tcaatcctcc aaaatggaaa
     1801 cctctactac aactaaaaag ggttgtccca tttatcacac cttcgtgttg accaatgagc
     1861 gcgttcacca atttaaatgt agagccgggt ggatatttag ccatagttgc tcgattgtac
     1921 aaaggcttga gcgtatcgac taacattttc cgatagttct tgctctttac acgaccaacc
     1981 aacaaattcg gatcataggt tgggctcgaa atcatggtca aaatttcacc tgtggaaggc
     2041 tcaattgcta caatagaacc cactttgttg accatgagct tttcgccata ttgctgcaat
     2101 tcagcatcaa tcgacgaagt aagactgcga cctaaaaccg aagcggtatc gtatttcccg
     2161 ttttcgaaac tccctttttc tctgttgtac acgtcaacca tgacgatttt cacacctcgt
     2221 tgtcctcgga gaggtttttc gtagcttttc tcgataccac tcaatcccat attatcacct
     2281 tggcggtagt atggattgtc tttgatcatc gattcgtcaa cttcacccac atagcctaag
     2341 gcatgagcag cggtgcgaag cggatattta cggagcgttc ttgcttgaac aaagaatcct
     2401 gggaatttgt aaagcttctc ttgtagggtt gcgtatgttt cgagtgataa ttcactctcg
     2461 aaaatttcag ggcgaaacga actgaacttt ttgcctttga gttcgattag ttttttcgtg
     2521 aaatcttctt tggtaatgtc gagcagcttg cacaaatcga gtgtgtcgat tgcttttacc
     2581 cgtttgggga tgaccatcag gtcgtacgca gcttcgttgt aaacgaggat ttttccgttg
     2641 cggtcgtata ttacgccacg ttcaggatat tcgattttgt gacgaagagc gttgtcgtta
     2701 gctgctagct tataacgatc gtccaccacc tgaatgtaaa acaggcgaat tagaaagatg
     2761 gccaccagag taatgaagat ggcgctgatg acgtatttgc gatccgaaaa cagattcatt
     2821 tggctacttc ccgattacgc tgtgtgagaa actgactgag gacagtgagc agaagcgtac
     2881 tgatgctgca ggtgatgacg cgcaacaaaa gttcgaaaaa attcaggaat gtaaaggctt
     2941 ccaccaaaaa taaaactaag tgatggatga ggattaaagt tgaagcatag ataaggaaag
     3001 tacttccgcc catacctgcc atattgagtt ttagcgtggt ttcgtatcct tcacgaggtg
     3061 cgattaagtg taaaaatccc ggacggaaaa aaccaagtac taaacaagcc gaagcgtgca
     3121 tacctgctgt ccctccaaaa atatctaccg ttaatcctgt tagaaagcaa ataaataaca
     3181 agaggatttt attgatatta actggtaaca tcagtaaaaa caagacatat aaattaggaa
     3241 tgataaagcc gttcaattct ccactgattt cgatttgatt caagactaaa acctgaacaa
     3301 aaatcagcaa gataaaccga atgagctgtg taattatctc tccaatcatt tcacatccag
     3361 cgttttggct tccaatgtat cacgttcggc tttcatcaag ttgcgtacaa catacacgta
     3421 cgacaacttc tgaaaatcgg tagataatcg tagtttgatt tctttaaaac tttcccccga
     3481 agttccaatt tcagaaaccc gtccaaccat aactccctgc ggataaatac ccgaaaatgc
     3541 actggttaca atagtatcac caacagtaac ttgagcatga ctaggaatat caaacaactg
     3601 tgcaaactgt ggagaatcac catcccatac agttgatcca caatagtcgt ttttcttgat
     3661 gcgagatgaa accttgtaat tactattcaa caccgacatt accgtactaa aatgctccga
     3721 aacatctttc acaattccta ccacacctgt acttccaatt acggcatcgt cgggttttac
     3781 accttgcaaa cttcctttat cgagtgtgat gtagttattt actcgagtaa ctgtattgtt
     3841 gattacgcgt gcgctgatgt attcgtattg ttgcaaacca agtgaatcgt tcactttcat
     3901 gatttcaaac ttcggatcgt aaaacgaaga tgaatcggct ttgcgtaaac gagcattttc
     3961 ggcagcaaga acacgattgg tttcacctaa aatcaagtaa tcggtcacat tactgtaggc
     4021 agcatacatc tgtccaacta gttcattact cgttcccaca attgcagcgc gttgatacgt
     4081 tgaattctgg acgagcaaaa cacctgaaac cacctccagc agaagaaaga caatgaagaa
     4141 attgtatttt ctgatgagga cgaaaaaatt ccgcatacga tctcagatca aaaatggaag
     4201 cttgattagc gaatcaggaa tttgaattta tcgcgatttt tcaaagcgat accagtacca
     4261 cgagcaacag cacgaagtgg atcttccgca atataaactg gaagtttagt acgagcagag
     4321 attcgtttat ccaaaccgcg tagcattgaa ccacctccag ctagataaat cccagtacgg
     4381 taaatatctg cagaaagctc tggaggagtc atttccaagg cattcaaaat cgccgtttcg
     4441 attttagcaa tcgatttatc caaacaatga gcaatctcct gatacgaaac catgatttct
     4501 tttggaatac cagtcatcat gtcgcgacca tgaacagcat aatctgcagg aggattttca
     4561 atttccgtta aggctgcgcc cacttcaatt ttgatacgct cagcagtacg ttcaccaatg
     4621 tgaatgttgt gttgacgacg catgtagtct tcaatatcag cagtgaaatc atcacccgct
     4681 acacgaatac tcttatcgca cacaattcca cctagggcaa ttaccgcaat ttcggaagta
     4741 ccacctccaa tatcgataat catattcccc attggctctt ctacgtcgat tccaataccg
     4801 attgccgcag ccattggttc gtggatcaag taaacttcct tagctcctgc atgttcagca
     4861 gagtcgcgta ccgcacgttt ttcaacctcc gtaattcccg aaggaataca aataaccatg
     4921 accaatgacg gagaaaaaat cttacgaccc ggattgatca tttttatcat cccgcgaatc
     4981 atgtgttccg ccgcattgaa gtcggcaatt acaccatctt taagcggacg gatagttttg
     5041 atgttttcgt gtgtcttacc atgcatttgc tgagcttgct ttccaacagc aattactttt
     5101 cctgttgaac gctcaatagc cacaatcgat ggctcatcca ccacaacttt gtcgttatga
     5161 atgataatcg tgttggcagt accaaggtcg atggcgattt cttgggtgaa gaaattgaat
     5221 aaacccatgt gtgtatttcg ggattaatgt ttaaagtgtc tgattcctgt aaaaaccatc
     5281 gccatattca agcggttggc tgtgtcgatg gtttcctgat cgcgaaccga tccgccaggc
     5341 tgcgcaatcg cagcaattcc agcctcggca gctaattcaa cattgtcgga aaatggaaaa
     5401 aatgcatccg acgacaatac tgatccttcg gtttgaaagc ccattcgacg cgctttttcg
     5461 accgattgtt ttaatgcatc cacacgagaa gtttgtccgg ttcctgaagc taaaagctgt
     5521 aagtcacgca caagaactat cgcgtttgat ttggtatgtt tcaccagaat nnnnnnnnnn
     5581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn accaaatcaa tttctggaat ggcaaattct
     5701 tctaattgag caatgtcgga ttgttcatcg cggcgactca aaattcctcc aaaaactttc
     5761 gggtgaagtg ttttcactcg tccgcccaaa atggaagggt aagaagtcaa atcttccaca
     5821 gcaataacct cgattcctaa atcctccaaa aaggcacgtg tgccaccagt agaataaata
     5881 gttaccccca aagcctttag tttcaaagcc aatggcgcta agccatcttt gtaaaacacc
     5941 gaaattaaag cactctggat gcgtttaggt tgggacatgg aatggaaaga atgaaggcgc
     6001 aaacttaatg aaaagaagtc catcttttag ggctattcgc atggaaagag aaggaaatgt
     6061 taagaatttt tgaacaaaga tgcttggttt ctaacatgct gaatttcaag gggtattttt
     6121 agcaattggc agattgggaa atgttggctg ttaaaacgat gactttttaa gtcctttcac
     6181 tcaagaattt tgtttttgct gatcgaaaaa gaaggtaatt atcagatata aactaaaaat
     6241 cagaaatccc ttctaacttt gcccaatgct gtttttacgc cttttcaagg aaagttttca
     6301 gtttgcatta gattcgctga ttagcaataa gcttcgtacg tttctctctt tgttgggaat
     6361 tacaatcgga atctttgcca tcatctcggt gtttactatg gttgattcga ttaagaataa
     6421 cctcgataaa agtatcgctt ccctcggaaa caatgtgatt tttatccaaa aatggccttg
     6481 ggaatttagt tccgattaca aatggtggga ttatatgaat cgtcccgtta cccaatatcg
     6541 tgaactagat ttggtacgtg aacgttcaca cttagcagct gcttcggctt ttcttattca
     6601 ggcaaatgtt acactcgagt ataaaagtgc atctgccgaa aatatccaga ttagcgccgt
     6661 gagcgatgaa tacgaacgca ttaagtcttt cgaaattcaa gaaggacgct attttagtga
     6721 tgcggaaatt caagcgggga gaaacaaaat tgtagtcgga gcagaggtcg ccactcaact
     6781 ttttggtaat atttcgccca ttggtaaaga gattttagtt cgtggtgatc gggtagaaat
     6841 aattggtgtt ttcaagcgcg aaggtcaaaa catcctcgat tttggcttgg ataacgctgc
     6901 catggttcct ttgaattttg ctcgtgcttt ggttgatatt cgaagcgata atgtgaatcc
     6961 atacattgct gtaaaagctt tggatggaat tagcaacgaa gctttaatcg atgaacttac
     7021 tggaattatg cgtggaattc gtcgcatcaa accgcgtgaa gccaataatt tcgcattgaa
     7081 tgaaaccaag ttaatttcaa acggactagg cagtttgatc ggagtagtca atgttgcagg
     7141 aggagtaata ggtttgtttt cgattttagt tggaggattt ggaattgcga acatcatgtt
     7201 tgtatcggtt aaggaacgta caaacattat cggaattcaa aaggctttag gcgctaaaaa
     7261 ctatttcatt ttattccaat ttctttttga atcgatgttg ctttcgattg ctggaggaat
     7321 aatcggatta atcatcatat tcctgctaac actcgcactg agtgccggat tagatatgga
     7381 tatttcctta agcttgaaaa atatcagctt gggaattatg atttcttcca ttatcggttt
     7441 aattgccgga attgctcctg cattatccgc ttcccgactc gatccggttg aagccatgcg
     7501 ttcgaaataa gctttcgttg cattcctgct tttgttctga acaatttttt cggagattcg
     7561 ttcttccttc aatccgctaa tcgtatgagt gataaattaa ttcgacgaag ttttcttgcc
     7621 cgactaagtg ctggattgag caccgttttt atggcaccat tagctgcgaa atcagagtcg
     7681 gtttctcatg cttttacctc taataatcct gaagtgaaac cgataaaatc tatcaagcca
     7741 ttaggttttc aatgggaaac cgccgatcca tttttgtttt gtgtacatca cgaagatgct
     7801 ttccccaaag gaaatgaggt gatgggccca gatcagcaat attttcaagg aagacactta
     7861 ggtgacgatt tcattattaa agatggattt cgcatgtatc atgggaagaa agtgcccggt
     7921 tttcctggac atccacaccg tggttttgaa accattactg tggtaaggaa aggatttgtt
     7981 gatcacgccg attcgatggg tgctgcggga cgatatggag atggagatgt gcaatggatg
     8041 actgccggaa aaggagttca gcattcagaa atgtttcctt tgttaaagaa ggattcagct
     8101 aatccgatgg aactttttca gatctggttg aatcttccga aaaggaataa aatggttgag
     8161 ccacatttta aaatgctctg gcaagaagcg attcccattt attctcacag cgatcgcaaa
     8221 caactcaata cacgaataga agttattgca ggcactgttg gagataagaa agcacttgct
     8281 ccaccaccta attcatgggc agccgatcct aaaaatgaag tggcagtttg gaatatcaaa
     8341 atgcaggctg gtgcaacttg gacaatgccc aaagcatccg cagggatcaa tcgtacctta
     8401 tacgtatatg aaggtagttc catcacactt gctggggaac ttgttaaagg atacagcgcg
     8461 gcagaagtac aagcagaaat ggatttactc attcaagcgg gagctgaaga agtgagtatt
     8521 ttggttttac aaggaagacc tattggtgaa acggttattc aatacggtcc ttttgtgatg
     8581 aatactaaag aagagataaa tcaagctttt gaagactatc atcgtactct ttttggagga
     8641 tggccttggc cttcgtctga tcaagtacat caacgtagtt taggacgttt tgctcgtcat
     8701 gctgacggga aagaagaa
//
LOCUS       DAOOQW010000081         8680 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_10014_length_8680_cov_4.960232, whole genome shotgun sequence.
ACCESSION   DAOOQW010000081 DAOOQW010000000
VERSION     DAOOQW010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 8680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8680
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10014_length_8680_cov_4.960232"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..673
                     /locus_tag="PK281_06160"
     CDS             <1..673
                     /locus_tag="PK281_06160"
                     /inference="COORDINATES: protein motif:HMM:NF036547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOY48964.1"
                     /translation="LFRKFAADFADKYKMPPIRLDEMSQQVLMKYRWPGNVRQLKNVT
                     EQISIIENERNVSPEVLDRYLPSPHGSMVPALSRQESSGDYSEREILYKVLFDMKKDM
                     VDMKKLMLGFLQNGVADQHFQEDNAQIISKLYNDIQQTPIYQNSSPTAATVPASPYIT
                     PANTPVTEHIEVEEESLSLENREKELIIKALEKYRGRRKNASKELGISERTLYRKIKE
                     YGINL"
     gene            675..1166
                     /locus_tag="PK281_06165"
     CDS             675..1166
                     /locus_tag="PK281_06165"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HOY48965.1"
                     /translation="MKRFWVLLFISVLALQSCKLSLSGGNYGEAKTISIAFFPNNAAL
                     VQPTLSQAFTEDMRNFFQTQSPLSLVQKGGDLHLEGAITDYRVGPVGVSSQTASTNRL
                     TITVNVKFTNRLDSTKDFDSSFSRFVDFPSSQNLVSVETELIRQINQQLIQDVFNKAL
                     INW"
     gene            1247..2023
                     /locus_tag="PK281_06170"
     CDS             1247..2023
                     /locus_tag="PK281_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48966.1"
                     /translation="MELYPYFQGAHILYALTLNSTNDIRFNEALRNAALHTGNRKVLF
                     QFLHEPLPTKTVEKEEIVENSFPLSLEVNSDLDFFSEPEEKGILEEDVFVNNQVEIAL
                     PNEQPEIALQEEPIIEPEISNTEDQSTPKIGKHSFSDWLKTIQTNKIVTEQEVSTSLR
                     EKEGVSVVDKEELIEKFIKTEPRISKPAKAEFFSPIQMAKKSVEDSDDIVSETLANIY
                     AAQGNPTRAIRIYQKLSLLNPDKSGYFAALIQKLENPDLL"
     gene            2020..2379
                     /gene="secG"
                     /locus_tag="PK281_06175"
     CDS             2020..2379
                     /gene="secG"
                     /locus_tag="PK281_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HOY48967.1"
                     /translation="MTGIISALIILASILLILVVLVQNSKGGGLSSTFAASNQVMGVR
                     KTTDFLEKSTWSLVATLVVLCLLSTVMNRSSEPKAQQSVSQEKAESATPTAPAKAPAA
                     APAPSAPAPAATPAPVK"
     gene            2530..2808
                     /locus_tag="PK281_06180"
     CDS             2530..2808
                     /locus_tag="PK281_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019975019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HOY48968.1"
                     /translation="MSDISIRPLQDRVLVEAAAAEEKTAGGIIIPDTAKEKPQKGVVV
                     AVGNGKKDEPMTVKVGDEVLYGKYAGTEISVNGKDLLIMRESDIFAIL"
     gene            2836..4473
                     /gene="groL"
                     /locus_tag="PK281_06185"
     CDS             2836..4473
                     /gene="groL"
                     /locus_tag="PK281_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004585059.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOY48969.1"
                     /translation="MAKDITFNLDARDQLKRGVDALANAVKVTLGPKGRNVIIDKKFG
                     APAITKDGVSVAKEIELSNPIENMGAQMLKEVASKTADIAGDGTTTATVLAQAIITAG
                     LKHVAAGANPMDLKRGVDKAVLLVVENLKSQSKSVGDDNQKIEQVATISANNDHTIGK
                     LIAEAMGRVKKEGVITVEEAKGTETTVDVVEGMQFDRGYISPYFVTDTEKMEAVLENP
                     YILIYDKKISTMKDLLPILEKSVQSGRPLLIIAEDVDSEALATLVVNKIRGSLRIAAV
                     KAPGFGDRRKAMLEDIAILTGGQLISEERGYKLENADLTYLGRAEKVTIDKENTIIVN
                     GAGAKEDIVSRVNQIKAQIENTTSDYDREKLQERLAKLAGGVAVLYVGAATEVEMKEK
                     KDRVDDALHATRAAVEEGIVPGGGIAYIRAIESLTGAKGINDDETFGINIVKRALEEP
                     LRQIVANAGGEGSIVIQKVKEGKEDFGFNARTEVYENLFAAGVIDPTKVTRVALENAA
                     SIAGMLLTTECVLSEIKEEAPAGGGMNPGMGGMGGMM"
     assembly_gap    4749..4848
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4849..5531
                     /locus_tag="PK281_06190"
     CDS             <4849..5531
                     /locus_tag="PK281_06190"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOY48970.1"
                     /translation="GNASYFASQLLSKTMKYDGGDSTYVFSGGFSGGLTGAFYFDHGG
                     YYSRKIYGIRLEVNYSRLNQSYRFFPGQGVIDPEVFYKYRLRLSYIDVPLMFTFCPTH
                     HQGLTVEAGPQISFLQSANAIAQESRNTEPLVPTINKQNFKPTTFSFVAGAGIFFSFS
                     ETFAMSGTFRAMYGLSSLIKSGTISTSATPDRRLTLGITVQALMKINKYDAKKNRGYK
                     YYLKRARH"
     gene            5537..6196
                     /locus_tag="PK281_06195"
     CDS             5537..6196
                     /locus_tag="PK281_06195"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOY48971.1"
                     /translation="MKVLLIGATGLTGSYVLDLLLQNERVSQVVVWGRSETGKKNPKL
                     KEVLVDFKSPIELASFPEVDVVICCLGTTIKKAGSQEAFWKTDVDIPERLAIAAKQKN
                     VSAFILQSSVGAAAGSSNFYLRCKGELEDKITQLNFISSASFRPSLLLGPRKEQRILE
                     QISKGIMVSFSFFFFGPLARYKAIPAEHVARAMVQFAIHPMEGNLVFEHSEIQRLSDL
                     L"
     gene            6663..8615
                     /locus_tag="PK281_06200"
     CDS             6663..8615
                     /locus_tag="PK281_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010181518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOY48972.1"
                     /translation="MITVGNLSVVFNGKPLFEDISFMIQQRDKVGLTGKNGAGKSTLL
                     KIISKQQEPTKGTVSVPSGYRIGYLPQEMKHATGKTIFEEARTAFDEVLSLEKELNDL
                     SHALETRTDYESDEYHEIIERLNDAGQRLSLLGGGSIDGETEKILLGLGFKSSDLHRS
                     MDEFSGGWKMRVELAKILLQMPDAVLLDEPTNHLDIESIQWLEDFLSSYPGAVVLVSH
                     DRAFLDAVTARTIEITLGKIEDYKASYSRYVVLRQERRDQQMAAYLNQRKMIEDTEKF
                     IDRFRAKASKAVQVQSRIKQLDKVERIEVEDEDNAAIHFRFPDAPRSGKVVLEAHHVH
                     KAFGEKVILKDLDFLVERGEKIAFVGRNGEGKTTLSKIITGHLEHTGKVEIGHNVKIG
                     YFAQNQAELLDPNATVLETLEQIARNITTQQLRSILGSFLFSGDTVEKKVRVLSGGEK
                     GRLALARLLLEPVNLLVLDEPTNHLDMRSKDILKGALRQYNGTMILVSHDREFLDGLC
                     NKVYEFNEGKIREFPGGIFEFLQSRKLENLKQLEVKKDITKAKAEVKQEQKAEAAANK
                     QVQQRGSDKEIKQLQTQIRNCENRVGELEAELKTAEEKLADPELYANQQESATFLNKY
                     ELLKKELDEQMVKWEELLARMEKIGG"
BASE COUNT         2827 a         1461 c         1892 g         2400 t
ORIGIN      
        1 gttgttccga aagtttgcgg ctgactttgc agataaatat aaaatgccac ccattcgatt
       61 ggatgaaatg tctcaacagg tattgatgaa atatcgatgg ccgggtaatg ttcgtcaatt
      121 aaaaaatgta acagaacaga tttctattat tgaaaacgag cgtaatgttt caccggaggt
      181 attagatcga tacctaccta gcccacatgg gagcatggtt ccagcactat cacgtcaaga
      241 aagttcaggt gattattcgg agcgagaaat attatataag gtcctttttg atatgaagaa
      301 ggatatggtc gacatgaaga aactcatgtt gggattcttg cagaatggtg ttgcagatca
      361 acattttcag gaggataatg cacaaatcat tagtaagtta tataatgata ttcagcaaac
      421 ccctatttat caaaacagca gtccaacagc agctacagtt cccgcaagtc catatattac
      481 tccagcaaac actcctgtaa cagagcacat tgaagtggag gaagagagcc tatcactgga
      541 gaatcgagaa aaagaactca tcattaaagc acttgagaag tatcgaggca gaagaaaaaa
      601 cgcatctaaa gaactaggaa tctcagaacg tacgttatat cggaaaatta aggaatacgg
      661 aattaatctt tgatatgaaa cgattttggg tgcttctttt tatttcagta ctagcgttac
      721 aatcgtgtaa attgtcgctt tcgggaggta attatggaga ggcaaaaact atatctattg
      781 cattttttcc taataacgca gccttggttc agccgacatt aagtcaggca tttaccgaag
      841 acatgcgaaa ctttttccaa acacaaagcc cactttcttt agttcaaaag ggtggagatt
      901 tgcatttaga aggagcaata accgattatc gtgttggccc tgttggtgtt agtagtcaaa
      961 ccgcatcaac gaataggcta accattactg ttaatgttaa atttaccaat aggttagact
     1021 caactaaaga tttcgacagt agtttttcac gttttgtaga tttcccaagt agtcagaatt
     1081 tagtgtcggt tgaaacggaa ttaattcgtc aaattaatca gcaattaata caagacgtat
     1141 ttaataaagc actaattaat tggtaatgaa taggggcgat tttctgtcat atttaagtaa
     1201 tccaacgaac gtattgcccg gcgcaagtcg agatttggag agcatattgg aattataccc
     1261 ttattttcaa ggcgcacata ttttatatgc tttaacatta aattcaacta atgatattcg
     1321 atttaatgag gcattgagaa atgcggcatt acacacgggt aacaggaaag tattatttca
     1381 atttttacac gaacctctcc ccacaaaaac tgtagagaag gaagagattg tagagaattc
     1441 tttcccttta agtttagagg taaattctga cttagatttc ttttctgaac ctgaagaaaa
     1501 ggggatattg gaagaagacg tttttgtaaa caatcaagtc gagatagcac ttccaaatga
     1561 gcagccagaa attgcacttc aggaggagcc aattatagag cctgaaattt cgaatacgga
     1621 agatcaatca acccccaaaa ttgggaaaca cagtttttca gattggttaa aaacaattca
     1681 aacaaacaaa attgtaactg aacaagaggt gtcaacaagt ttgcgtgaaa aggagggtgt
     1741 tagtgttgtg gacaaagaag agttgataga aaagtttatt aaaacagagc ctagaataag
     1801 taagccagca aaggcagaat ttttctcgcc gatacaaatg gctaagaaaa gtgttgagga
     1861 ttcggatgat attgtttcgg agacattagc taatatttat gctgctcaag gaaatccaac
     1921 tcgggcaatt cgtatctatc aaaaattaag tttgttaaat ccggataaaa gcggttactt
     1981 tgccgccctt atccaaaaac tggaaaaccc tgatttgtta tgaccggtat aatttccgca
     2041 ttgataatcc tagcatcaat ccttttgatt cttgttgtac ttgtacaaaa ctctaaagga
     2101 ggaggccttt catctacgtt tgcagcaagc aatcaagtga tgggagttcg taaaacaaca
     2161 gactttttag agaaatctac ttggtctctt gtagcaacat tagttgtttt gtgtttgctt
     2221 tctacagtaa tgaatcgttc atcagaaccc aaagcacaac aatcggttag tcaggagaaa
     2281 gcggaatcag caacaccaac agcacctgca aaagctcctg ctgcggcacc agctccttct
     2341 gcacctgcac cagcggcaac tccggctcct gtcaaataaa atattcttaa ttgaacgaac
     2401 gtgtcagtct gtcaccagat tgacacgttt tttttttgca tttaaccgaa caactgacaa
     2461 gacttccaca accgttgact ggcatatcat tcgctaagcg gcgcccgtaa actgtaaaaa
     2521 aataaaataa tgtctgatat ttctattcgt ccgcttcagg acagagtact tgttgaagcc
     2581 gcagctgcgg aagaaaaaac agccggagga attattattc ccgatacagc taaggagaaa
     2641 ccacaaaaag gagttgtggt agctgttggc aatggaaaaa aagacgagcc aatgactgta
     2701 aaggttggtg atgaagtttt atacggcaag tatgctggta ctgaaatttc tgttaatggt
     2761 aaggacttgt tgattatgcg cgagtccgac atttttgcaa ttctctgata gaactcactt
     2821 cgaaatacta aaacaatggc aaaggacatc acctttaatt tggacgcacg tgatcaattg
     2881 aaacgcggcg ttgatgcatt agctaatgct gtaaaagtaa cattaggccc taaagggcga
     2941 aatgtaatta ttgataaaaa atttggagct ccagcaatta caaaggatgg agtttcagtt
     3001 gcaaaagaaa tcgagttgag caatccaatt gaaaacatgg gagctcaaat gttgaaagaa
     3061 gtagcatcaa aaacagctga tattgctgga gatggaacaa caacagcaac ggtattggca
     3121 caagcgatta ttacagctgg attaaaacac gttgcagcag gagcgaatcc catggatttg
     3181 aagcgcggtg ttgataaagc agttttattg gttgttgaga atttgaaaag ccaaagcaaa
     3241 tcggttggag atgataacca aaagattgag caagtagcta ccatttcggc aaacaatgac
     3301 catactattg gaaagttgat tgctgaggcg atgggaagag tgaagaaaga aggagttatc
     3361 acagtagaag aagcaaaagg aaccgaaaca acagttgatg ttgtagaagg tatgcaattc
     3421 gaccgcggat atatttctcc atatttcgtt actgatacag agaaaatgga agccgtattg
     3481 gagaatccat acatcttgat ttacgacaag aaaatctcaa ccatgaaaga cttgcttcca
     3541 atattggaaa aaagtgtaca aagtggtcgt ccattgttga ttatcgcaga agatgttgat
     3601 agcgaagctt tggcaacctt ggttgtaaat aaaatcagag gatcacttcg aatcgcagca
     3661 gttaaggctc ctggatttgg agatcgccgt aaagcgatgt tagaagatat tgcaattcta
     3721 actggtggac aattgatttc tgaagagcgt ggttacaaat tagaaaatgc ggatttgaca
     3781 tatttaggtc gcgcagagaa agtaaccatt gacaaagaga atacaattat tgtgaatgga
     3841 gcaggagcta aggaagacat cgtttcacgt gttaaccaaa ttaaagctca gattgaaaac
     3901 actacatcag actacgatcg cgaaaaactt caagagcgtt tagcgaaact tgcaggaggt
     3961 gtagctgttc tttacgtagg tgctgcaacc gaagtggaga tgaaagagaa aaaagatcga
     4021 gttgatgatg ctcttcatgc aactcgtgct gccgttgaag aaggaattgt accgggaggc
     4081 ggaattgctt atatccgtgc aattgaatct ttgactggag caaaaggaat caacgatgat
     4141 gaaacgtttg gaataaacat cgttaaacgt gcactagaag aacctcttcg ccaaatcgtt
     4201 gcaaatgcag gtggagaagg atctatcgta atccaaaaag taaaagaagg aaaagaagat
     4261 tttggattta atgctcgtac tgaagtttat gagaatttgt ttgctgcagg agtaattgat
     4321 ccaacgaaag taactcgtgt agcacttgaa aatgctgcat ccattgccgg aatgttgctt
     4381 actacagaat gtgttctttc tgaaattaaa gaagaagcac ctgctggagg aggaatgaat
     4441 ccaggtatgg gaggcatggg aggaatgatg taattattca accccattaa accttataaa
     4501 caccctgtac tccatggagc tacagggtgt tttgtttaca agcttatcga ctttcttaca
     4561 agtaggcaga aaatgaatcc aaatgtgctt atttttcgta ggttagccag actcgtgaga
     4621 tttatacccg caatttttct ttttatcttc ttaattaaag gaagtggttt aggagcccag
     4681 acctatcagt tcggtggcaa tgcttcctat tttgcatctc aactgttgtc taaaacgatg
     4741 aaatatgann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngt ggcaatgctt
     4861 cctattttgc atctcaactg ttgtctaaaa cgatgaaata tgacggcggt gattcaacat
     4921 atgttttttc tggaggattt agcggcgggt taactggagc tttttatttt gaccatggag
     4981 gatattattc ccgaaaaatc tatggtattc ggttagaggt taattactct cgtttgaatc
     5041 aatcgtatcg ttttttcccc ggccaaggtg ttatagatcc agaggttttt tataaatatc
     5101 gcttaaggct ttcttatatt gatgttcctc tgatgtttac tttttgtcca acgcatcatc
     5161 aaggattaac agtagaagct ggaccacaaa tcagcttttt gcaaagtgct aatgcgatcg
     5221 ctcaagagtc tagaaacacc gaacctttgg tgccaactat aaacaagcaa aattttaaac
     5281 caacgacctt tagttttgta gctggagctg gaatattttt cagtttttca gagacattcg
     5341 ccatgtcagg aactttccga gcaatgtatg ggttgtcttc tctaattaag tctggcacca
     5401 tttcaactag tgcaacacca gatcgaagat taactcttgg tattactgtt caagcattga
     5461 tgaagattaa caagtacgac gcaaaaaaga accgaggata taagtactat ttaaaacgcg
     5521 ccagacattg aaagaaatga aagttctttt aattggggca acagggctta cgggctctta
     5581 tgtactagat ttattattgc aaaatgagcg agtttctcaa gttgttgttt ggggaagatc
     5641 cgaaaccggc aaaaagaatc ctaagctcaa agaggtttta gtagacttta aatcaccaat
     5701 tgaattagca tcatttccag aagttgatgt agtaatttgt tgcttaggaa cgactattaa
     5761 gaaagccggt tcgcaagagg cattttggaa aaccgatgtg gatattcctg aacggcttgc
     5821 aattgctgca aaacagaaga acgtatctgc atttatttta caatcgtcgg ttggcgcagc
     5881 agcaggctcc agcaattttt atcttcgatg taaaggagag ttggaagata aaattaccca
     5941 gttgaatttt atttcgagcg caagttttcg tccatcttta ctgttaggac cacgaaaaga
     6001 gcaacgaata ttggaacaga taagtaaagg gattatggta tccttttcat ttttcttttt
     6061 cggtccatta gcgcgctata aagcaattcc tgcagagcat gttgctagag cgatggtgca
     6121 atttgccatt cacccaatgg aaggaaattt agtttttgaa cactccgaaa ttcaacgttt
     6181 atctgacttg ttgtaaacag gatctttggt tttgtcgtat ttaacaaaga tgtttaggta
     6241 cagacttctt gcccacctga aaagcagtgg agcaaacaag attagcgaca atgtgtttag
     6301 aataaaaaag cgggttgtat gaaacccaat caacacattt attagcaaaa ccgcgacact
     6361 tagagcgaca cctaatgcgt aactaacata catcgcacct tgaaaaaaac caggttcgcg
     6421 ttcaaaatgc agtttgcagg tagggcaatc aggatgcatt ttatcccaat tttccatttt
     6481 atacggattg ttgtttaaga acatcttgct ttcgtgacat cttgggcatg tacatttaat
     6541 tattgagtac aacttacttt cacgaaacgc catcctgttt tttcggcaaa gttacagatt
     6601 aatcgttcct tatacagact attctaccga taaacagaaa tggatgacct acctttgcgg
     6661 caatgattac agtaggaaat ttatcagtag tttttaacgg aaagccgtta tttgaagaca
     6721 tctcttttat gattcagcaa agagataaag ttggtttaac gggaaagaac ggagcgggga
     6781 aatcgacact tctaaaaata attagcaagc aacaggagcc aacaaaagga acagtttcgg
     6841 ttccatcggg atatcgaatt ggctatttgc ctcaggaaat gaaacatgct acaggcaaaa
     6901 ccattttcga agaagcgcgg actgcatttg atgaggtgtt gtcgttagaa aaagaactaa
     6961 acgatttaag tcatgcgttg gaaacacgaa ctgactatga atcggatgaa tatcatgaaa
     7021 ttattgagcg acttaacgat gctggacaac gcctttcttt attaggcgga ggaagtatcg
     7081 atggtgaaac agagaagatt cttttaggac tagggtttaa atcaagcgat ttacaccgat
     7141 cgatggatga attttctgga ggatggaaaa tgcgagtaga attggctaaa attctacttc
     7201 aaatgcctga tgccgtattg ttagatgagc cgacgaatca cttggacatt gagtcgattc
     7261 agtggttaga ggattttcta tcgagttatc ctggagcggt tgtattggta tctcatgaca
     7321 gggcattttt agatgcagta actgctcgta cgattgaaat cacattaggg aaaatagagg
     7381 attacaaggc ctcttattct cgttatgtgg ttctccgcca ggaaagacgt gatcaacaaa
     7441 tggctgcata tctgaatcag cgtaagatga ttgaagatac ggagaagttt atcgatcgat
     7501 ttagggctaa ggcatccaag gctgttcagg ttcaatcgcg catcaagcaa ttagataaag
     7561 ttgaacggat tgaagtagaa gatgaggata atgctgctat tcactttcgg tttccggatg
     7621 ctccgcgttc aggaaaagtt gtgctagaag cccatcatgt gcataaagca tttggagaaa
     7681 aggtaattct aaaggattta gattttttag ttgaacgagg agagaagatt gcttttgtag
     7741 gtcgaaacgg tgaaggaaaa accaccttgt cgaaaatcat taccgggcat ttagaacata
     7801 ctggaaaagt tgagattggg cataatgtga agattggata cttcgctcaa aatcaagcag
     7861 aattgttaga tccgaacgca actgttttag aaacgcttga acaaattgcc cgaaacatca
     7921 ccacacagca attgaggagc attttaggtt ctttcctgtt tagtggagat accgtagaaa
     7981 agaaggttcg agttttatcg ggaggagaaa agggacgtct agctttagct cgattattac
     8041 tagaaccagt taatttgtta gttctggatg agcctacgaa tcacttagat atgcgttcaa
     8101 aagacatcct gaaaggagct ttgcgtcagt ataatggaac gatgattttg gtgtcgcacg
     8161 accgggagtt tttagatgga ttgtgtaata aagtatatga gtttaatgaa ggaaaaatcc
     8221 gagaatttcc gggtggaatt tttgaattcc ttcaaagcag aaagcttgaa aacctcaaac
     8281 agttagaagt aaagaaagac attactaaag cgaaagcaga ggttaaacaa gagcaaaaag
     8341 cagaagctgc agcaaataag caagttcaac aacgaggaag cgacaaagaa atcaaacagc
     8401 tccaaacaca aattcgaaac tgtgaaaatc gggtaggtga attagaggcg gaattaaaaa
     8461 ctgctgaaga aaaattagcc gatccagaac tttatgcaaa ccaacaggag tctgcaacat
     8521 ttttgaataa atacgagttg ttgaaaaagg aattggacga gcaaatggta aagtgggagg
     8581 agttattagc tcgtatggaa aaaattggcg gatgattaac gaatcgcgaa cggtaatttt
     8641 atttgatggc gtatgtcatt tatgtaataa ttgggttcgc
//
LOCUS       DAOOQW010000082         8667 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_10039_length_8667_cov_5.345680, whole genome shotgun sequence.
ACCESSION   DAOOQW010000082 DAOOQW010000000
VERSION     DAOOQW010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 8667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8667
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10039_length_8667_cov_5.345680"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="PK281_06205"
     CDS             <1..358
                     /locus_tag="PK281_06205"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY48973.1"
                     /translation="EYRKVVISPADQQVDLISFQLTLNGVMDVVEDPTGIEETSVLGF
                     QIYPNPGHSIVTLTQAKDQEAILIVRDLSGREQIRQVITARQTSIDLSLLSSGAYLIE
                     YRNANNQSEYARWIKE"
     gene            453..998
                     /locus_tag="PK281_06210"
     CDS             453..998
                     /locus_tag="PK281_06210"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HOY48974.1"
                     /translation="MDYLRQYNVQFSGLSEDVHEFQYEIKDAFFKEFDNAVVDGGDIQ
                     VDLELDKQTNLMTLNFRFAGIVQVMCDRCAAVNPYPVEGKSRLILKLGEGNADDDELV
                     ILPRGEYEYNIAQHLYEEICLSLPLRVVPCEVTGDTSMCDQSVIDRLENMLVDELPEE
                     GEEEAIDPRWEKLRNLNKETE"
     gene            1029..1220
                     /gene="rpmF"
                     /locus_tag="PK281_06215"
     CDS             1029..1220
                     /gene="rpmF"
                     /locus_tag="PK281_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HOY48975.1"
                     /translation="MAHPKRKISKTRRDKRRTHYKAELPTLAKCSTTGTIHLFHRAYF
                     VDGALYYKGKLVVEAKTNA"
     gene            1260..2204
                     /gene="plsX"
                     /locus_tag="PK281_06220"
     CDS             1260..2204
                     /gene="plsX"
                     /locus_tag="PK281_06220"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HOY48976.1"
                     /translation="MRIGLDIMGGDFAPQATIGGAVLAVDAIDASCRLVLIGDEPAIR
                     MSFLEVGIDPNRFDYVQATEVIEMGENPTRAFTQKPNSTISTGFRLLKEGEIDAFVSA
                     GNTGAMLVGSVLSVKPIDGIQRPCIMSLMPKENGGFGILLDVGSNADCKPETLYQFAL
                     MGSMYAANVLGISNPKIGLINIGEEPEKGNELSKAAYQLMNNSADFNFIGNIEGRDLF
                     NDKADVMVCDGFTGNVVLKLAESFHSLFAKRGFSDPYIDRFNYENYGGTPVLGINSTV
                     IVGHGISNAPTIKNMITLAQEVVSAGLTDKIKAVLQHD"
     gene            2197..3192
                     /locus_tag="PK281_06225"
     CDS             2197..3192
                     /locus_tag="PK281_06225"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HOY48977.1"
                     /translation="MTKLRAAITGVHGYVPEYILTNKELEKMVDTTDEWIMTRTGISQ
                     RHILKGEGLGVSDMAVEAVKGLLAKTNTKAEDIEVVIFCTVTADMVFPASANILSDKI
                     GMKNAWGFDLGAACSGFLYGLTTGSRLIESGAYKKIIVVGGDKMSAIVDYTDRATCVI
                     FGDGCGAVLLEPSVDENGVMDSILKSDGSGREFLHQKAGGSVKPATHATIDAKEHFVY
                     QEGKSVFKFAVTNMADVSAEIMERNHLTSDDIAWLVPHQANKRIIDATASRMGVGSDK
                     VMINIEKYGNTTSGTIPLCLWEWEKQLKKGDNIILAAFGGGFTWGAVWIKWAYNS"
     gene            3252..3737
                     /gene="accB"
                     /locus_tag="PK281_06230"
     CDS             3252..3737
                     /gene="accB"
                     /locus_tag="PK281_06230"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002680803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HOY48978.1"
                     /translation="MDIREIQDLIKFVAKSGVSEVSLETDDFKLTVKTETSKSGKETI
                     VYQVPAEVQAPVAVAAPAPASQAPAAPAPAPKAEVPSNTVTIKSPMIGTFYRSPGPDK
                     ALFVNVGDEIAPGKVICIIEAMKLFNEIESDVKGRIVKVLVDDASPVEYDQPLFLVEP
                     A"
     gene            3770..5110
                     /gene="accC"
                     /locus_tag="PK281_06235"
     CDS             3770..5110
                     /gene="accC"
                     /locus_tag="PK281_06235"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014165709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HOY48979.1"
                     /translation="MFKKILIANRGEIALRIIRTAHEMGIKTVAVYSTADKESLHVRF
                     ADEAVCIGPPASKDSYLNIANIISAAEITNADAIHPGYGFLSENAKFSKICQDHNIKF
                     IGATPEQIEKMGDKSMAKTTMREAGVPTVPGSEGLLESLEDAKRTAKKIKYPVIIKAT
                     AGGGGKGMRIVWKEDELENAWDSARQEAAASFGNDGMYMEKYIEEPRHIEIQIIGDQY
                     GRVSHLSERDCSIQRRHQKLVEETPSPFMTQELRDEMGDAAVRAAKAVNYEGVGTIEF
                     LVDKHRNFYFMEMNTRIQVEHPITEEVIDYDLIREQIKVAAGVPISGKNYYPQMHAIE
                     VRINAEDVNNNFRPSPGTITTFHAPGGHGVRLDTHAYAGYTIPPNYDSMIAKLITIAQ
                     TREEAIAKMRRALHEFVIDGVKTTIPFHKKLMENADFINGNYTTKFLEGFEFKD"
     gene            5157..6005
                     /locus_tag="PK281_06240"
     CDS             5157..6005
                     /locus_tag="PK281_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48980.1"
                     /translation="MMKKYFALVFILYSGITQAQTKLFQPGWQFSIGTGTNLDRWDKG
                     LDSLNTFFQGDPNTLGAIPAQPALRIYNKPSYNLELNYIHNLQSRFTWKAGINAGQQK
                     LSYTLGDSYVDSNSIKLSSAGLNALAICSFHPSDGSTLMIGVGAGVQGLLLKDNEIYT
                     RIDTLLNISGFADLHAESTLNMNLTSSVVIQGIAQIEWINKLNDRWSISASAQARIPF
                     NDFFTFSGESNLTSTIEFFGQTITTPISSTTRTSSPSNMPYWQFNIGIVRLLEMKRMI
                     SVDKAL"
     gene            complement(6002..7132)
                     /locus_tag="PK281_06245"
     CDS             complement(6002..7132)
                     /locus_tag="PK281_06245"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOY48981.1"
                     /translation="MFRFIGLILICFLQQLQAQVTFIISDLPASTSKPIRLFAAGSFN
                     QWNPADSLYAFHLNARSEYELVCTDTGMIEFKITQGNWESAEGDQDGKAIPNRKYRVK
                     NKEIINLKIAGWERSSTNKSTALSNVQELKSFQNYPNGLPKRKIWVCLPESYSQSETL
                     RYPVIYMQDGQNLFDDSTSFSGEWGLDEYLKSLGPNTFQEAIIIGIENSSNRTLEYTP
                     WQPDSMKGGEGFRYIDFVEHQLKPHIDSVFRTLPEAKFTCIGGSSLGGLISLTAALQS
                     PHVFGRAMVFSPAIWINHDSLLTWVKTLNLENGPALFFCAGDSESRTMLLFVDELIPI
                     LKKCGLKSERIFYRKVEGGRHSESTWKVEFSKAWNWISNQYD"
     gene            complement(7132..7923)
                     /locus_tag="PK281_06250"
     CDS             complement(7132..7923)
                     /locus_tag="PK281_06250"
                     /inference="COORDINATES: protein motif:HMM:NF007757.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOY48982.1"
                     /translation="MNLSISMVQTRLFWQDRQQNLDWFDSRLELITGQTDIIILPEMF
                     TTGFSMNASSIAEEMGGPAMQWMAKKAAEKNAVIVGSMMMKESGSFYNRLIWMNPDGR
                     FEYYDKRHLFRMANEHEHYSSGKSRVIFQHKGWKICPLICYDLRFPVWSRNRMVKEEY
                     DYDVLIYVANWPERRAHAWKSLLVARAIENQAYVVGVNRIGTDGNEVIYSGDSALIDY
                     MGERLSRTERFGDRCETVVISKSELDDFRKSFPAAMDADQFTIQA"
     gene            complement(7927..>8667)
                     /locus_tag="PK281_06255"
     CDS             complement(7927..>8667)
                     /locus_tag="PK281_06255"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOY48983.1"
                     /translation="VFFQLDPQSFKVNWNEFQKLVNPRTRMIIINTPHNPTGSMLNAA
                     DIQRLEKLTQNTDIIILSDEVYEHILFDGYEHQSVARFPKLAQRSVIISSFGKTYHTT
                     GWKLGYCLAPANLMAEFRKVHQYLVFSVNTPLQYAYADFLKEKDRYLHLPAFYQEKRD
                     FFNRLFEGSRFSFQPSAGTYFQLLNYQAISNEKDVDFARRLTIEHGVASIPVSVFYHK
                     VVDNKLLRFCFAKNNDELERAAEKLIKL"
BASE COUNT         2633 a         1757 c         1799 g         2478 t
ORIGIN      
        1 ggaatatcgc aaagtggtga tcagtcctgc tgaccaacaa gttgatttga ttagtttcca
       61 attaacctta aatggtgtaa tggatgtggt tgaagatcca actggtatag aagaaacttc
      121 tgttttagga tttcaaattt atccaaaccc aggtcattca attgtgaccc taactcaagc
      181 gaaagatcaa gaagcaattc tgattgtgcg tgatttaagt ggacgtgaac aaattcgaca
      241 agttattact gctcgtcaaa ctagcatcga cttaagttta ttgagttcag gagcctatct
      301 aatcgaatac cgtaacgcta ataatcaaag cgaatacgcc cgttggatta aggaataaag
      361 gcaattctac tttcgataaa tgaatatttt acattccact tcattggaag aaaatttcta
      421 ttatctttgc cctcccttaa aaaagccctg atttggatta tctacgtcaa tacaacgtcc
      481 agttctccgg tttaagtgag gatgtccatg agtttcagta tgaaataaag gacgcgttct
      541 ttaaggaatt tgataatgca gttgtggatg gtggcgacat tcaggttgat ctggaattag
      601 ataaacagac caatttgatg acgttaaact tccgtttcgc gggtattgtg caggtaatgt
      661 gcgatcgatg tgcggcggta aatccatatc cggtggaagg caaatctcgc ttgattttaa
      721 agttgggaga aggcaacgcg gatgatgatg aattggtaat tctgccgaga ggcgaatacg
      781 aatacaacat tgcacagcat ttatacgaag aaatttgtct ttcacttccc ttgcgcgttg
      841 tgccatgtga agtgactggc gatacaagca tgtgtgatca atcggttatc gaccgtttgg
      901 aaaacatgtt agtagatgaa ttacctgaag aaggtgagga agaagcgatt gatccacgtt
      961 gggagaaatt gagaaacctc aacaaagaga ctgagtaagt atttttttga acgattatat
     1021 aagaacccat ggcacacccg aaacggaaaa tttcgaagac ccgccgcgat aaacgcagaa
     1081 cacactacaa ggctgaattg cctacattgg cgaagtgttc tacaaccgga acgattcacc
     1141 tctttcaccg cgcctacttt gtagacggag ccctttatta caaagggaaa ctagtggtag
     1201 aggccaaaac caacgcctga taattcctga ggtctaacgg ctgaacgggg aagatatcca
     1261 tgcgcatcgg cctcgatatc atgggagggg attttgcccc tcaggctacc attggcggag
     1321 ctgtgctggc ggttgatgct atagatgcat cctgccggct tgttctcatt ggggatgagc
     1381 ctgctattcg tatgtccttt ctcgaagtcg gtatcgaccc aaatcgtttc gattacgttc
     1441 aggctaccga agtaattgaa atgggtgaaa atccgactcg ggcatttacc caaaaaccca
     1501 attcaaccat tagtactggc tttcgccttc ttaaagaggg tgaaattgat gctttcgtta
     1561 gtgccggcaa tacaggcgct atgcttgttg gttctgtctt aagtgtaaaa cctatcgacg
     1621 gaattcaacg cccttgcatc atgtcactta tgcctaaaga aaatggtggc tttggtattt
     1681 tactcgacgt aggctcaaat gccgattgta aaccagaaac actttaccaa tttgctttaa
     1741 tgggatccat gtatgcagcc aatgtgctgg gtatctctaa tcctaaaatt ggtcttatca
     1801 acattggaga ggaacctgaa aaaggaaacg aactctccaa agccgcttat cagttaatga
     1861 ataattctgc cgatttcaac ttcataggaa atatcgaagg acgagactta ttcaatgata
     1921 aagccgatgt gatggtgtgt gatggcttta caggtaatgt ggttctcaaa ctagctgaat
     1981 catttcacag tttatttgca aaacgtggat ttagtgaccc ttacatcgat cgtttcaatt
     2041 acgagaacta cggcggaact cccgtattag ggataaattc aactgtaatt gtaggtcatg
     2101 gtatttccaa tgctcctacc attaaaaata tgatcaccct agcgcaagag gtagtaagcg
     2161 ctggacttac cgataaaatc aaagcagtac ttcaacatga ctaaacttcg cgcagccatt
     2221 accggcgtcc acggatatgt acctgagtac atcctcacca acaaagaatt ggagaaaatg
     2281 gtggatacga cggatgaatg gatcatgacg cgaacaggaa tctctcaacg tcacatatta
     2341 aaaggtgaag gtttaggtgt ttctgatatg gctgtggaag cagttaaagg acttctagcc
     2401 aaaaccaaca caaaggcaga agatatcgaa gtagttattt tctgtacagt tactgccgat
     2461 atggtttttc ctgcatcagc aaacattctt tccgacaaaa ttgggatgaa aaacgcttgg
     2521 ggctttgact taggcgcagc ttgtagtggt tttctgtatg gattaactac tggttctaga
     2581 ttgattgaat cgggtgctta caaaaaaatc atcgtagttg gtggagataa aatgtctgcc
     2641 attgtcgatt ataccgatcg tgctacctgt gtgatttttg gagatggttg tggtgcagtt
     2701 ttattagaac caagtgtaga tgaaaacgga gttatggact cgattttaaa atcggatggt
     2761 tcaggacgcg agtttttaca tcaaaaagct ggaggttcag ttaaacctgc tactcatgca
     2821 accattgatg caaaagagca ttttgtatat caagaaggaa aatcagtttt caaatttgct
     2881 gtaacgaaca tggcagatgt gtcggccgaa atcatggaac gtaatcacct tacttctgat
     2941 gatattgctt ggctagttcc acatcaagcc aacaaacgta ttattgatgc tacagccagc
     3001 cgcatgggtg taggaagtga taaagtaatg atcaacatcg agaaatacgg aaatacaact
     3061 agcggaacca ttccactttg cttgtgggag tgggagaaac aacttaaaaa aggagataac
     3121 attattctag ctgctttcgg tggtggattc acttggggag cagtttggat taaatgggct
     3181 tataactcct gagtttgtcg cacacataat tttatatatt taccgcctca tcacccaaac
     3241 accgaatatc tatggacatc cgggaaatcc aggatcttat aaagtttgtc gccaagtcgg
     3301 gcgtgagtga agtatcactc gaaaccgatg atttcaaact tactgtcaag acagaaacat
     3361 ctaaatcagg aaaagaaacc attgtttatc aggttccagc cgaagttcag gcacctgttg
     3421 cagtggctgc tccagctcca gcaagtcagg ctcctgctgc tcctgcacct gctcctaaag
     3481 cggaagttcc ttcaaataca gtgactatta agtcgcccat gattggaaca ttctaccgct
     3541 ctccaggacc agacaaggct ctttttgtga acgtgggcga cgaaatcgct ccaggcaagg
     3601 tgatttgtat catcgaagcc atgaaattgt tcaacgagat tgaatcagat gtgaaaggac
     3661 gtattgtaaa ggtgttggtg gatgacgcct caccagtaga atacgaccaa cctctgttcc
     3721 ttgttgaacc agcatgaacc accgtttaaa cggttaaaac ctacgtaacg tgttcaaaaa
     3781 aatcctgatc gccaaccgcg gcgagattgc tctacgcatc atccgcacgg cccacgaaat
     3841 gggaattaag accgtagcgg tatattcgac tgcagacaaa gaaagtctac atgttcgttt
     3901 tgccgacgag gctgtttgta ttggtccacc ggccagtaaa gattcctatt taaatatagc
     3961 caatatcatc tctgcagctg agataaccaa tgccgatgct attcatccgg gctatggttt
     4021 cctttcagaa aatgctaagt tttcgaaaat ttgccaagac cataacatca aattcattgg
     4081 tgctactcct gagcaaattg agaagatggg tgataaatcc atggctaaaa caaccatgcg
     4141 tgaggctgga gtacctacag ttccaggttc cgaaggtttg ttggaaagct tagaagacgc
     4201 taaaagaact gccaaaaaaa tcaagtatcc agttatcatc aaagctaccg ctggtggtgg
     4261 tggtaaaggg atgcggattg tttggaaaga agatgaactt gaaaatgcct gggactctgc
     4321 tcgtcaagaa gctgcagcct catttggaaa cgatggcatg tacatggaaa agtacatcga
     4381 ggaacctcgc cacattgaaa tccaaattat cggtgaccaa tatggacgtg ttagtcatct
     4441 aagtgaacgt gattgctcga ttcaacgtcg tcaccaaaaa ttggtcgaag aaacaccttc
     4501 accatttatg actcaagaat tgcgcgatga aatgggtgat gcagccgttc gtgcagctaa
     4561 agctgttaat tacgaaggtg ttggaacaat agaattcttg gttgataaac accggaattt
     4621 ctatttcatg gaaatgaata cgcgtattca ggtggaacat cctattactg aagaagtaat
     4681 tgactacgac ttaattcgtg agcaaatcaa agtggcagct ggtgttccta tttcgggtaa
     4741 gaactactat ccgcaaatgc acgccattga agttcgtatc aatgctgaag atgtgaacaa
     4801 taacttccgt ccttctccag gtacaattac tactttccat gctccaggtg gacatggtgt
     4861 tcgtctcgat acgcatgctt atgcaggtta tacaattcca ccgaattacg attcgatgat
     4921 tgctaagctt atcaccattg ctcaaacacg tgaagaagct atcgccaaaa tgcgcagagc
     4981 tttacatgaa tttgtcattg atggtgtgaa aacaaccatt ccttttcaca agaaacttat
     5041 ggaaaatgcc gatttcataa acggtaatta caccactaag tttttagaag gattcgaatt
     5101 caaagattaa taaacacaaa aaccagagta ctcctctggt ttttttatgc cataatatga
     5161 tgaagaaata ctttgcatta gtattcattc tgtacagcgg aataacccaa gctcaaacaa
     5221 agttatttca acccggttgg caatttagca taggcactgg aaccaattta gatcgctggg
     5281 ataaagggct tgattcatta aatactttct ttcaaggaga tcccaatacg ttaggagcaa
     5341 ttcctgcgca acctgcattg cgtatctata ataaacccag ttataatctc gaactcaatt
     5401 atattcataa cctacaatct cgatttacat ggaaagctgg aataaatgca ggtcagcaga
     5461 aattaagtta tacgttgggt gatagttatg tggattcaaa ttccatcaaa ttaagctcag
     5521 caggattgaa tgcattagcc atttgctctt ttcacccaag tgatggtagt accttaatga
     5581 ttggtgtagg cgcaggtgtt cagggtttgt tgttaaaaga caatgaaatt tatacgcgca
     5641 tcgataccct attaaatatt tcgggttttg cagatttaca tgctgagtca accttaaata
     5701 tgaatttgac aagcagcgtt gttattcaag gaattgctca aattgagtgg ataaataaac
     5761 taaatgatcg ttggagtatt tcggcatcag cacaagctag aattcctttc aatgatttct
     5821 tcactttctc tggagagagt aatttaacaa gcacaattga atttttcgga caaacaatta
     5881 ctactcccat ctcttcaact accagaactt caagcccgag taacatgcct tattggcaat
     5941 tcaatatagg gattgttaga ctattggaga tgaaacgaat gatatctgtt gacaaagcgc
     6001 tttaatcata ttggttcgaa atccagttcc aggctttgct gaattcgact ttccaggtgg
     6061 attctgaatg tctcccgcct tctactttcc ggtaaaatat ccgctccgat ttcaagccgc
     6121 attttttcag tatcgggatt aactcatcca caaataaaag catcgttcta ctctcagaat
     6181 ctcctgcaca gaagaataat gcaggaccat tttctaagtt taacgttttt acccatgtga
     6241 gaagcgaatc gtgattgatc caaatggctg gtgagaaaac catagctcta ccgaaaacat
     6301 ggggtgattg taaagcagca gtaagagata ttaatccacc aagcgaactt cctcctatgc
     6361 aggtgaattt agcttcaggt aaagttctga ataccgaatc gatatgaggt tttagttgat
     6421 gttcgacaaa atcaatatac ctaaatcctt caccaccttt catcgaatcg ggttgccaag
     6481 gtgtgtattc cagtgtgcga ttggagctat tctcaattcc aatgatgatg gcttcttgaa
     6541 acgtatttgg gccaagcgat tttaagtatt catctaaacc ccattcacct gaaaaagagg
     6601 tggaatcgtc gaataaattt tgtccatcct gcatataaat cacaggatat cgtaaggttt
     6661 cagattgaga atatgattcg ggtaaacata cccatatctt ccttttggga agtccattcg
     6721 gataattctg gaaactttta agctcttgaa cattcgacaa ggctgtgctt ttattagttg
     6781 aagatctttc ccaaccagca atcttcagat ttataatttc tttgtttttt actcgatact
     6841 tccgattcgg gatagctttt ccatcctgat ctccttcggc agattcccaa ttaccttgag
     6901 taatcttaaa ctcaatcatt ccagtatctg tacaaactaa ttcatattca cttcgtgcat
     6961 tcaaatgaaa ggcataaagc gaatcggcag gattccattg attgaacgaa cctgcagcaa
     7021 ataatcgaat cggtttcgac gtcgacgcag gcaaatcact aatgataaat gtaacctgcg
     7081 cttgaagctg ttgcaggaag caaatgagta tcagcccaat gaaacgaaac atcaagcttg
     7141 aatagtaaac tgatccgcat ccattgctgc tggaaatgat tttcgaaaat catctaactc
     7201 gcttttcgat atcaccaccg tttcgcatcg atcgccaaag cgttctgttc gacttaaacg
     7261 ctcgcccatg taatcgatta atgccgaatc gcccgaataa attacctcgt taccatcagt
     7321 tccgattcga ttaactccta ccacatatgc ttgattttca atcgctcgag cgactaataa
     7381 agatttccat gcatgtgcac gtcgctcagg ccaattagct acgtaaatca acacatcata
     7441 atcgtattct tctttaacca ttcgattacg tgaccaaacc gggaaacgta aatcatagca
     7501 tatcagtgga catattttcc atcctttatg ttggaagatt acacgcgatt tacccgaaga
     7561 ataatgctca tgttcattgg ccattcgaaa taaatgtcgc ttatcgtaat attcaaatcg
     7621 accatcggga ttcatccaaa tgagtcggtt gtaaaacgaa cccgattctt tcatcatcat
     7681 gcttcccaca atgacagcat tcttttctgc tgctttcttg gccatccatt gcatagctgg
     7741 accgcccatt tcttcggcaa tagaagaagc attcatactg aagccagtcg tgaacatttc
     7801 gggtagaata ataatatcgg tttgtcccgt tatcagttcc aagcggctat caaaccaatc
     7861 gagattttgt tgtctgtctt gccaaaacaa acgggtttgc accatactga tggataaatt
     7921 catagcttat agtttgatga gtttctcagc agctctttca agctcatcgt tgtttttagc
     7981 gaagcagaaa cggagtagtt tattatcaac caccttatga taaaacactg aaacaggaat
     8041 ggaagcaaca ccatgttcta tggttaatcg gcgcgcaaaa tccacatctt tctcattcga
     8101 gattgcttgg tagttaagta gttgaaaata agtaccagca gaaggttgaa agctaaaccg
     8161 cgatccttca aataatcgat taaagaaatc acgcttttct tggtaaaaag ctggtaaatg
     8221 aagatagcgg tccttctctt ttagaaaatc agcataagca tattgcaaag gtgtattaac
     8281 cgaaaaaacc aagtattgat gcaccttacg gaattcggcc ataagatttg caggtgctaa
     8341 acaataaccc aatttccaac ctgtagtatg atacgttttt ccaaaggatg aaatgatgac
     8401 tgaacgttgc gccagcttgg gaaaacgagc tacactttga tgttcatagc catcaaacag
     8461 gatgtgctca tatacttcat cactcaaaat aatgatatcg gtgttttgcg taagcttctc
     8521 cagtcgttga atgtcagcag catttaacat gcttccggtt ggattatgag gtgtgttgat
     8581 gatgatcatc cgagttcttg gattcaccaa cttttgaaac tcattccaat tgactttaaa
     8641 actctgagga tctaactgaa aaaaaac
//
LOCUS       DAOOQW010000083         8648 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_10075_length_8648_cov_5.314209, whole genome shotgun sequence.
ACCESSION   DAOOQW010000083 DAOOQW010000000
VERSION     DAOOQW010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 8648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8648
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10075_length_8648_cov_5.314209"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1373)
                     /locus_tag="PK281_06260"
     CDS             complement(<1..1373)
                     /locus_tag="PK281_06260"
                     /inference="COORDINATES: protein motif:HMM:NF040164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HOY48984.1"
                     /translation="MRNYLFLIPYLILVFIFQSELNAQSDFILYNQTYNPHKTSVNPA
                     FLPKEEFYLGLPLISNSYANFSNNSFTYRDLIKKKTGTDSIYFDFDGFVQGLSKTNYI
                     TASLQTDLINFGWREGRWYVNGNITEKVSLQTQFNKQLMEVAVYGNGPQIGQTVDLGN
                     LFVTATHYREYGLGLSRDVNCHLRLGATAKYLYGMENLDVSKSLIKLSTAPVTFDLSG
                     TADILIHTSGLNNFDQDSIKRGSYLFGRKNRGYSLDLGAEYKFNDRYSLFASILDIGR
                     IRWTYQPENYYTTVPKFSFTGLPLNQFLSTNTDTISNSVQHYLDSLGNIFKLKTQNES
                     YVSHLPTRYYLGGHYTFTDRGAFQFLLYGTSYKSKLYPSVSLGFSKRFNDVLEFSVNG
                     SYHNKTFLNVGAGFTLNLGLTQFTIITDNLVGLMGQYTSRGTNLRAGITLVTGYDAKR
                     PNYCDTDG"
     gene            complement(1376..2893)
                     /locus_tag="PK281_06265"
     CDS             complement(1376..2893)
                     /locus_tag="PK281_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48985.1"
                     /translation="MTAKDILANDDSPTVISATDEGIVEIIYSSLSTTERASELIFLP
                     LVEESNTFGPSSTNFNAFNSNQTLGFSIEDSVQYSIPYSLDNQLGSTSRLDTVFFKSG
                     KLNLDIQSVIPHTSAVTVEIPNLYINNQIYRQVVPLIYTNQPTTSTQIERNLTGAYLV
                     PNPGTETITIKYKFTVVRENAQSIPTINCLSSDLSFTDVSFGKLSGLFGNYSASLSQN
                     DTLFMHIFRNVISAQNLQFINPKARIVVSNSTGIPIDYTQNSFSAFRPGTSIPSVDIS
                     GFSFPDLIAAQEHTSTLASQYSYAFEQAIGSNISTVINLFPRYLLSRGNYAFNYTQPT
                     ASYFLRDTSRVSVYSEVSLPLDGLTLDLQVRDTLEFQFKSISQDVEEIMLRLNIINGF
                     PTDGNLQVYFCKQLPGSNQAIQIIDSLYVQGTEPVLLPGETGANGTVTIPSQKITDAI
                     IDRQKWLKLYEFEADRILIKARLTTYDLGQLAVKVLENDELKIRISARIKAHKSF"
     gene            complement(3093..3716)
                     /locus_tag="PK281_06270"
     CDS             complement(3093..3716)
                     /locus_tag="PK281_06270"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010133955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOY48986.1"
                     /translation="MKKIELLSSYLSGKIEAGVDEAGRGCLAGPVVAAAVILPPDFRH
                     PFLTDSKQLSEKKRDQLRPIIEQEAISWAIGKASAAEIDSINILQASFLSMHRAIEQL
                     NQVPELLLIDGNRFKKYRQVEHVTVIGGDGKYLAIAAASILAKTERDAEMKLLHQEFP
                     AYCWDRNMGYGTQKHRDAIREIGPCIHHRMSFTLLPNQLSLFSNSEE"
     gene            3780..5579
                     /locus_tag="PK281_06275"
     CDS             3780..5579
                     /locus_tag="PK281_06275"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY48987.1"
                     /translation="MKQLLLLCILLGLNFSAQAQCTACLIDNGCTANPLAPALCPDVL
                     TDAVQGQAYEIDITFFIPQQFYNESAGVNVTLSQITITSVSGLPNGLSWTASEADNTY
                     DITSDPATQRGCVKICGTPDAIGSYTIAVNVIATVTAPISTTSPQTFTLPLLVVPGGG
                     GNSGFSFEPSSGCDSLNVDFQGLITSTTQPVSYLWDFGNGNTSTEQFPPTQTYALPDT
                     YYVSLQTDLLNYVLESVSFNATGSNWCGDIEEPSIPFIGTCTGSPDIYFQYTVGSSSQ
                     QSATIDDNTSMSLSGLGYVINEPSFSLSFFDEDVISANDDLGSIVIQITNPGTYNFNT
                     SQGYGSYTIGTQVGLSFTNIDTVFVFDSPQTPALSVNDTLVCDGDSVTITVDSASFYQ
                     WYAEGEPLFGSNSSSITTYVTGTFQVEVRNATGCAAFSNVVNIEVAEMPDVPTVFFNP
                     ITNEFFCNPSNGLSWIWLIDGVEMAGSTNLVSFLPTIIGNYSVIVENEFGCSAQSLPV
                     FFTNVGLNEAADRLKFRVYPQPFKSGSLTIEGVDKPANVTISDASGRRVYQTQINGNS
                     AQYIQMPELNAGIYFVQIQTSEKAGSRMLSVIK"
     gene            complement(5572..6276)
                     /locus_tag="PK281_06280"
     CDS             complement(5572..6276)
                     /locus_tag="PK281_06280"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOY48988.1"
                     /translation="MAYRILLAEDEESLSEVIQLNLELEGYQVIPVTNGKSALERALN
                     ERFDLIILDVMIPLIDGFSVCEKIRLENQEVPIIFLTAKNTSSDRVQGLKLGADDYLS
                     KPFNLEELLLRVQSLIKRGLDRARVNTETSEYSFGNNRIDFSSYTITTFRNESITLSK
                     KEIQLLKLLIDRKGEVVSRRQILEKVWGYDIFPSTRTIDNYILAFRKYFEQNPREPEF
                     FHSIRSVGYKFTDKLI"
     gene            6421..8226
                     /locus_tag="PK281_06285"
     CDS             6421..8226
                     /locus_tag="PK281_06285"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HOY48989.1"
                     /translation="MKYFLTLLFSISIFATHAQRSCGTMELYQAASQQNPQLIQNRVQ
                     IENQIQSWISTQSNSGSRSIISIPVVVHVVYKTAGENISDSQVQSQIDVLNEDYSRTN
                     LDASQTPSMFQGVASNCEIQFCLAQRDPDNQPTNGITRTQTLASSFPLGASVKHSNTG
                     GKDAWPSDKYLNIWVCNLVNPVIGFATLPGTTSPDEDGVVIIYKHFGRIGNVEAPYNK
                     GRTATHEIGHWLNLLHTWGDDESSSDPCAGSDQVNDTPNQGGPNFGCPAAGTASCSNG
                     GDMFMNYMDYTDDNCMNLFTEGQKNRMIATLNGIRSTIPVSNGCITPPIIQDCDTLNN
                     ITGGDGLVYYYSYEIVETDSGFFTGTNSRNDLAFAEAHSVVGQHTVDAIRFDFAYAVD
                     GSGTSNVHVGIWEDDGTNPPGSPGTLVGEAFYPLAQVENNVDNFTFTDVAIAGSPLVD
                     GTFYVGFFTSDLAEDTIAVYSNQIDKVNVNTAWLQDQDGQWDTFSSAETFGNALSIAA
                     RPVVCETVGISEPLVNQLKLYPNPSTGKLNVQLPNYSVSQAWQVFAMDGSLVQEGKSS
                     SNTITVDLTNATDGIYLFRTVQGKDILSSRISVIR"
     gene            8285..>8648
                     /locus_tag="PK281_06290"
     CDS             8285..>8648
                     /locus_tag="PK281_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48990.1"
                     /translation="MQTIIEATTDTELQRIASKVYNKERILPAEGIYLYEHAELGYLG
                     ILANHIREDLHGKKTFFNRNFHIEPTNICVFDCKFCSYSRLLKHKQGGWELSEEDILE
                     KVRSYQGVPITEVHIVGGV"
BASE COUNT         2569 a         1655 c         1644 g         2780 t
ORIGIN      
        1 ccgtctgtat cgcaatagtt cgggcgtttg gcgtcgtacc cagttactaa agtaattccc
       61 gctcggaggt ttgttccacg actggtgtat tgtcccataa gaccgaccag attatcggtg
      121 atgatggtaa actgagtgag gccgagattt agcgtaaagc cagccccaac atttaaaaag
      181 gtcttgttat gataagaacc attcacgcta aattcaagaa catcgttaaa ccgctttgaa
      241 aagcccagtg aaacggatgg ataaagtttg cttttatagc tcgttccgta tagtaagaat
      301 tgaaatgcac ctctatctgt aaacgtgtaa tgaccaccta aatagtatct ggttggtaaa
      361 tgagatacat aactctcatt ctgtgttttc agcttaaaaa tattacccag tgaatcgaga
      421 taatgctgga cactattgct gattgtatcg gtattggtcg aaaggaactg atttaatggt
      481 aagccagtaa aggagaattt aggaacagtt gtgtaataat tttcgggttg ataggtccat
      541 cgaattcgac ctatatccaa aatactggca aaaagactgt atcggtcgtt gaatttatat
      601 tctgcaccta gatcaagcga gtaacctctg ttcttgcggc cgaataagta gctgccacgt
      661 tttatcgagt cttgatcaaa gttgttcaac cctgaagtat gaatcaaaat atcggctgtt
      721 ccacttagat cgaaggtgac aggagctgtg cttagtttta ttaggctctt gctcacatcc
      781 aaattttcca ttccatacag atacttcgcg gtagcaccta atcttaaatg acagtttaca
      841 tctcgcgaaa gacctaatcc atattctcgg taatgtgtgg ctgtgacaaa taaattaccc
      901 aaatcaacag tttgccctat ttgcggacca ttgccataaa cagcaacttc cattaattgc
      961 ttgttgaatt gtgtttgcag ggaaactttt tcggtgatat ttccatttac ataccaacgc
     1021 ccttctcgcc agccaaagtt tattaaatcg gtttgcagag aagcagtgat gtaattggtt
     1081 ttggatagtc cttggacaaa tccatcgaaa tcaaaataga tagaatcagt cccagttttt
     1141 ttcttgatta aatcccgata cgtgaagcta ttattagaaa aattcgcata actattcgat
     1201 atcaaaggca agcctaaata gaattcttct ttgggtaaaa atgcgggatt tacagatgtt
     1261 ttatgcggat tatacgtttg attgtacaag ataaaatcac tctgtgcatt caattccgat
     1321 tggaatataa aaacaagaat taaataagga attaagaata aataattccg cataatcaaa
     1381 atgatttatg tgctttgatg cgtgcggata ttcgaatctt taattcatca ttttctaaaa
     1441 ctttaacagc caattgccct aaatcataag ttgttagcct tgcttttatc aggattcgat
     1501 cagcctcaaa ttcgtaaagt ttaagccatt tttgtcggtc gattatggca tcggtaattt
     1561 tctgactagg tattgttacc gttccattcg ctccagtttc ccctggtaaa aggacaggtt
     1621 ctgttccttg aacatacagt gaatcaataa tttgaatagc ttgattactt cccggtaatt
     1681 gcttgcagaa atacacttgt aaattcccat cagttggaaa tccatttatt atattcaagc
     1741 gaagcatgat ttcttctaca tcctgagaaa tcgatttaaa ctgaaattct aaagtgtctc
     1801 gaacttgtaa atcgagtgtc aatccatcca gtggtaggga aacttcactg taaacactta
     1861 ctcgactcgt atctcttaag aaataacttg ctgttggttg ggtataatta aacgcataat
     1921 tcccacgact caataaatat ctcggaaata gattaattac agtcgaaata ttcgaaccaa
     1981 tagcttgctc aaatgcgtaa ctgtattgag atgcaagcgt tgatgtgtgt tcttgggcgg
     2041 ctattaaatc gggaaacgaa aatccactta tatcaaccga tggaatactt gttccgggac
     2101 gaaatgcact aaaactattc tgtgtataat cgataggaat tccagtcgaa ttactcacca
     2161 caattcgagc tttgggatta atgaattgaa gattctgcgc acttatcaca ttccggaaaa
     2221 tgtgcatgaa taaggtgtca ttctgcgata aacttgccga ataattacca aataatccac
     2281 tcaatttccc aaaggaaaca tctgtaaaac ttaaatcact tgacaagcaa tttattgttg
     2341 gaatagattg agcattttca cgcacaacgg taaacttata tttaatggta atcgtttctg
     2401 ttccagggtt cggaaccaaa tacgcaccag ttaaattccg ttcaatttgt gttgaagttg
     2461 ttggttggtt ggtatagatt aatggaacaa cctgacgata aatctgatta ttgatgtata
     2521 gattcggaat ctcaacagta accgctgaag tgtgaggaat aacgctttgt atatctagat
     2581 ttagttttcc tgatttgaag aatactgtat ctaaacgtga ggttgatcca agttgattat
     2641 caagtgagta ggggatagag tattggacag aatcttctat cgaaaatcct agtgtttgat
     2701 tagaattaaa tgcattgaaa ttagtgctac tcggaccaaa cgtatttgat tcctcaacta
     2761 ggggtagaaa aatcaattca gaagctcgtt ccgttgtact caaactggaa tagataatct
     2821 ctacaattcc ttcatctgta gctgaaataa ctgtcgggga atcatcattt gccaatatat
     2881 ctttggcggt catggttgaa tttaccaatg gaaaggctaa atctggattc cattccgatt
     2941 gtgctagttt agaaaagtca tcttttaagc aaccatgcaa aaccaacaat aggatcaaac
     3001 acagaggcca gaagcgtgct attagttttg gattgggttg catcaggact gaatctgctt
     3061 aaaagtactt gtttaattgt attaactagc ggtcattcct ctgagttact aaacaagctc
     3121 agttgattag gtaaaagtgt aaaactcata cgatggtgaa tacatggtcc aatttcacga
     3181 atggcatctc ggtgtttttg ggttccatat cccatatttc tgtcccaaca ataagcgggg
     3241 aattcttgat gtaaaagctt catctcggca tcacgttcgg ttttagctaa tatcgatgca
     3301 gctgcaatgg ctaagtattt tccatctcca ccaatgaccg ttacgtgttc aacttgccga
     3361 tattttttaa atcgatttcc atcaatcagt aataattcgg ggacttgatt taattgctct
     3421 attgctcggt gcatagataa aaaactagcc tgcaaaatgt ttattgagtc aatttcagca
     3481 gcactcgctt ttccaattgc ccatgaaata gcttcctgct caatgattgg tcgaagctga
     3541 tctcgctttt tttcactcag ctgtttcgaa tcagttaaaa agggatgtcg gaaatcgggc
     3601 ggtagaatta ctgccgcagc tactactggt ccagctaaac aacctcgtcc agcttcatca
     3661 acgccagctt caattttacc agataaataa ctgcttagta gttctatttt tttcattccg
     3721 ctaaatttac aatttgtcgg ctggctttaa tcttttgtta cattggattt tatttgtcta
     3781 tgaaacagct tctacttctt tgcattttac taggactcaa tttctctgct caagcacaat
     3841 gcaccgcttg tttaattgat aatggatgca ccgcaaatcc tttggcacca gctctttgcc
     3901 cggatgtgct taccgatgct gttcaaggac aagcctacga aatcgatatt acttttttca
     3961 ttccacagca gttttataat gaatcagctg gagtgaatgt aacactctct caaattacaa
     4021 ttacctccgt ttcgggtttg cctaatgggt tgtcttggac agccagtgaa gccgataata
     4081 cctatgatat cacatccgat ccagctactc agcgtggctg tgtaaaaatt tgcggaacgc
     4141 ccgatgccat tggttcatac accatcgctg taaacgtaat tgcaacagtt actgctccta
     4201 tttcaactac ttctcctcaa acatttacgt tgcctttatt agttgttcct ggcggaggag
     4261 gaaattccgg ttttagcttt gagccttcat ccggatgcga ttcattgaat gttgatttcc
     4321 aaggtttgat aaccagtact acacaacctg tttcgtattt gtgggatttt ggaaatggga
     4381 atacttctac cgaacaattt cctccaactc aaacctacgc tttacctgat acatactacg
     4441 ttagcttgca aaccgactta cttaattatg tgctcgaaag tgtgagtttt aacgccacag
     4501 gttcaaattg gtgcggcgat attgaagaac catctattcc ttttattggt acctgcactg
     4561 gatctcctga tatctatttt caatatacag ttggttcatc ttctcaacaa agtgccacaa
     4621 ttgacgacaa tacatctatg agtctttctg gattgggtta tgtgattaac gaaccttcat
     4681 ttagcctatc ttttttcgat gaagatgtta tatcagctaa tgatgattta ggatcaatcg
     4741 tgattcaaat taccaatcca ggaacctata atttcaacac cagtcaaggt tacggttctt
     4801 atacaatcgg aacacaagta ggactttcat ttacaaatat cgatactgtt tttgtattcg
     4861 actcaccaca aacaccagcg ctttcagtta atgatacgtt agtgtgtgat ggtgattctg
     4921 ttacgattac tgtcgattca gcctcgtttt atcagtggta tgctgaggga gagccacttt
     4981 tcggttctaa tagttcatcc attacaactt atgttactgg aacctttcaa gttgaagttc
     5041 gaaatgctac aggatgcgca gctttttcga atgtagtaaa tattgaagtt gctgaaatgc
     5101 ctgatgttcc tactgtcttt ttcaatccaa taacgaacga attcttttgc aatccttcta
     5161 atggtctatc gtggatttgg ttaattgatg gagtcgagat ggctggaagt actaatctgg
     5221 tttcgtttct tccaactatt ataggtaatt actcagttat agttgaaaat gaatttggat
     5281 gttctgccca atcactaccc gtatttttca ccaatgttgg attgaacgaa gcggctgatc
     5341 gcttgaaatt ccgcgtgtat cctcagccat ttaaatcggg aagcttaact attgaagggg
     5401 ttgataagcc tgcaaatgtg acgatttcgg atgcatctgg tcggcgtgtt taccaaactc
     5461 aaatcaacgg aaatagcgcg cagtatattc aaatgccaga attaaatgct ggaatttact
     5521 ttgttcaaat tcaaacttcc gaaaaggctg gatctcgaat gttgagtgtg attaaataag
     5581 cttatcggtg aatttataac caacacttct gatggagtga aaaaactccg gttctcttgg
     5641 gttttgttcg aagtatttgc gaaatgctag gatgtaatta tcaatcgttc gtgtagatgg
     5701 aaatatatca tagccccata ctttctcaag tatttgtcgg cgggatacta cttctccttt
     5761 tcgatcaata agcagcttta atagctgaat ctcttttttc gataaagtga tagattcatt
     5821 tctaaatgtg gtaatcgtat aactcgaaaa atcaattcga ttatttccaa aactatattc
     5881 cgaagtttcg gtatttactc gtgcgcgatc caagccacgc ttgattaatg attgaacacg
     5941 aagcagtaat tcttctaaat taaatggttt ggataaatag tcatctgcgc caagttttaa
     6001 tccttgtact cgatcagaac tagtattttt tgcagtgaga aaaatgatcg gaacttcttg
     6061 attttctaac ctgatttttt cacaaaccga aaaaccatca attaacggaa tcatcacatc
     6121 taaaatgatc aaatcgaatc gctcattcaa ggctctttct agtgctgatt tgccatttgt
     6181 cactggaatt acctgatagc cttctagttc taaattaagc tgaatcactt cacttagact
     6241 ttcttcatcc tcagccaata aaatccgata tgccatgctg caaagtaaca ggaaacaaat
     6301 tcatgttcac aactccattt taatgactgg attataaaac ttcatctggg taaattcgta
     6361 cagaataatt tttgacacga atttctatca aatctgctta attcatctag ccactattct
     6421 atgaagtatt ttttgacttt acttttttcg atttccattt ttgccacaca tgcacaacgt
     6481 tcttgtggca ctatggaatt atatcaggct gctagtcaac aaaatccaca attaatccaa
     6541 aatcgagtac aaattgaaaa tcagattcaa tcttggattt caactcaatc aaattcagga
     6601 tcacgttcta tcatttcaat tcctgttgtt gttcatgtgg tttataaaac agcaggagaa
     6661 aatatttcag atagtcaggt tcaatctcaa atagatgtgt taaacgaaga ttattcacgt
     6721 acaaacctcg acgcatctca aacaccatcg atgtttcagg gagttgcttc taattgtgaa
     6781 atacagtttt gtttagctca acgtgatccc gataatcaac caaccaatgg aatcacacga
     6841 actcaaacat tagcatcatc atttcctctt ggagcatcag ttaagcatag caatactggt
     6901 ggaaaagacg cttggcctag cgataaatac ttgaatattt gggtgtgtaa tcttgtaaat
     6961 ccagttattg gttttgcgac tcttcccgga acaacttctc ccgatgaaga tggcgtagtg
     7021 attatctaca agcattttgg tcgaataggt aatgtcgaag caccttacaa taaaggaaga
     7081 actgctaccc acgaaattgg tcactggtta aatttattac atacttgggg tgatgatgaa
     7141 agtagctccg acccttgtgc cggttccgat caagtaaacg atactcctaa tcaaggagga
     7201 cccaatttcg gttgtcctgc tgccggaaca gcttcttgta gcaatggtgg tgatatgttc
     7261 atgaattaca tggattatac cgatgataac tgcatgaact tgttcaccga aggtcaaaaa
     7321 aataggatga ttgcgacttt aaacggtatt cgatctacta ttccagtttc taatgggtgt
     7381 attactcctc caatcattca agattgtgat actttaaata atataacggg tggagatggt
     7441 ttggtttact actattctta tgaaattgta gaaacggatt cgggattttt tactggtact
     7501 aattcacgta atgacttagc atttgctgaa gcacattcgg ttgttggcca acacaccgtt
     7561 gatgcaattc gttttgattt tgcttatgcg gtagatggtt ctggaacaag caatgttcat
     7621 gtgggtattt gggaagatga tggtacaaat cctccgggaa gccctgggac acttgtaggt
     7681 gaagcatttt atccattagc acaagtggaa aacaacgtcg ataattttac gtttaccgac
     7741 gttgccattg ctggtagtcc attggttgat ggcacttttt atgtcggatt ttttaccagc
     7801 gatttagcag aagatacaat tgctgtttat tccaatcaga ttgataaagt gaatgttaat
     7861 actgcttggc ttcaagatca agatggacaa tgggacactt tcagctctgc cgaaactttt
     7921 ggaaatgcat tatcaattgc tgcacgccca gttgtgtgtg aaacggtagg gatttcagag
     7981 cctttagtta atcaactgaa actatatcca aatccttcaa caggaaaatt aaatgttcag
     8041 ctaccaaatt attctgtttc tcaagcttgg caggtttttg cgatggatgg aagtctagtt
     8101 caagagggaa aaagctcttc aaataccatc actgtcgatt taacgaatgc taccgatgga
     8161 atctatctgt tccgaaccgt tcaaggaaaa gacattttat catcaagaat ttctgtaatc
     8221 cgttagtccg aaataactat ttgctgcttg gcttgttgta agtttgtcga acctattttg
     8281 aggaatgcaa actatcatcg aagcaactac cgataccgaa cttcagcgta ttgccagcaa
     8341 ggtttataat aaggaacgta tacttccagc agaaggaatt tacctatatg aacacgccga
     8401 attaggatat ctaggaattc tagctaacca cattcgtgaa gacttacacg gtaagaaaac
     8461 gtttttcaac cggaactttc atatcgaacc aactaacatt tgtgttttcg attgtaaatt
     8521 ctgttcatac tctcgtttgc taaaacacaa gcaaggaggt tgggaactta gtgaagaaga
     8581 tattcttgag aaagtaagaa gctatcaagg agttcctatt accgaagtac atattgtagg
     8641 cggagttc
//
LOCUS       DAOOQW010000084         8529 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_10265_length_8529_cov_4.371607, whole genome shotgun sequence.
ACCESSION   DAOOQW010000084 DAOOQW010000000
VERSION     DAOOQW010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 8529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8529
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10265_length_8529_cov_4.371607"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2957
                     /locus_tag="PK281_06295"
     CDS             <1..2957
                     /locus_tag="PK281_06295"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOY48991.1"
                     /translation="IFCTFLLILLSSNYLNAQNTLVYTEDFENGPGVFGLNTTGEIGT
                     NAGQNKWVVNNLFTGTPIYPNTISQDSTTGGLINFAPFSNYLHIQDSVAASTQNIGNA
                     NFQPNAASDRFTSTSDFCTLGLDSVRIAFYYTCEGNPGASAQLYYLADGGAWTPVPGA
                     VFNNQTKWNYIEFYDEGFDNKNALRFGFRWTNDGSGGPTSGSMGVDGVRIVGKYAPDI
                     YNVRLELDSISPNPVCRGDGLLLFFSNPVPLCGTGFYEVQFSSAFGNFTNYTSLGIYQ
                     LNNQNTQQILFTLPIPSNTNASPCYNIRVMRVDITPVIVSDTSICIEVINCPNQIITL
                     QPAVLSNPTDTLCIGSVIDVPFYSLGVFQNNLYVAQLSDSNGLFPPNPNVLGFVSDNT
                     TYPPGTIPKGNVPGLVRPQNQPIPPGCNYYIRVISSNPTTIGSVYGPFCIRECDIETN
                     EKVDVQFCIDDVNGGDTLLTVDVGVFPPPATYSPPNEFQIQLLDFQSFGVINTGVVGS
                     VVAVNDTVVQLSIPNLPNLFTVGLIPGNYYMRIVATNSDQNWDLSGTLVRLTIGAPNP
                     SPLSIGIIDINTFLNVPFDGDTTICVNEAIYFLLSPYNFSSSYVWSLNNDPNFFEGGP
                     YNPILFNGLGDYTLGVTETNFGCVGPGSNDAIIRVQGFPSASIVGPAQVCLGDTATFS
                     VPLNQNTYYSWTTNPGSIVDSLSNTASISFPSSGTASIHMEAVNECGSQESTKTVFVR
                     QPPLVVTSNDTSVCVDAQLTLNTADGINFKFYWSEDGQLISQDREITVNPDTTTTYVI
                     RVTNYGSLACESADTVTVTVQYPDTGSVKTIDLCQGESATLQSDSTGISYLWSTGLLT
                     QSITVSDSGWYYVMINQANELCPMLDSFKVNVKVCYQPLELPNVFSPNGDGMNDVFTP
                     GQTYMYEDFNIVIYNRWGLKVYESMNPFFEWNGQDLNGNQLTDGTYFYIAKLEHHENS
                     DLQKGTVTILGK"
     gene            complement(2989..3471)
                     /locus_tag="PK281_06300"
     CDS             complement(2989..3471)
                     /locus_tag="PK281_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48992.1"
                     /translation="MKASFLKYLPLFSLITSSTFMFAQTESSTGFKAQKSDQEQKILE
                     RGKNYDVNFDIANHEAFFLEGEDSLFRFLYNRLTISQGAIDANLVANAMIGFKVNFDG
                     KVQQAYSIGQVGFGIDEQLIAELNKLEFVPASQGSMPYRSEIVLEIPIKAAYLAGMKK
                     "
     gene            complement(3549..4424)
                     /locus_tag="PK281_06305"
     CDS             complement(3549..4424)
                     /locus_tag="PK281_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004917393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOY48993.1"
                     /translation="MIKPETSLMLRDNALAGKNIVVTGGGTGLGRSMTKTFLQLGARV
                     LITSRKEEVLKNTCAELEKETGGFIRYAVCDVRKMDEVEAAIEKAWTELGPVNVWVNN
                     AAGNFISPTERLSHKAFDTIVDIVLKGSYNCTLAVGKRWIAEKIEGSFLNIVTTYAWT
                     GSGYVVPSATAKAGVLALTRSLAVEWAKYGIRSNAIAPGPFPTEGAWSRLFPEELAKK
                     IDPLARIPLKRFGEHPELGNLAAFLVSDFANFMNGEVVTIDGGEWLQGGGEFNNLEHV
                     PAPMWDIIEKAVRGK"
     gene            4479..5201
                     /locus_tag="PK281_06310"
     CDS             4479..5201
                     /locus_tag="PK281_06310"
                     /inference="COORDINATES: protein motif:HMM:NF026121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4476 domain-containing protein"
                     /protein_id="HOY48994.1"
                     /translation="MIFRTLLAALFTQAAFFSSAQMASLTLSLQDSSLFTAELNGTSY
                     SDASNVMNISRITPGPQKLKVIKLMQMGSSIVKKPVYDGTINLPAGKTTVAYIDQYNQ
                     FRISSTPPVSSTSSGSSNQTSSNSSPYFVPDPSAIKAYTQPKTTAQGMSNDQFAGKME
                     VLRSINQENQRYKTARDFISLGTYNSNQIAEMMLTLNNEHNRVNLADMGYEYAVDKEN
                     YGVVFNALRRPSSVRKLTRRLN"
     gene            complement(5251..6027)
                     /gene="mazG"
                     /locus_tag="PK281_06315"
     CDS             complement(5251..6027)
                     /gene="mazG"
                     /locus_tag="PK281_06315"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014165328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HOY48995.1"
                     /translation="MSTQLEKEQAFTRLLTIMDELREQCPWDKKQTLESLRHLTIEET
                     YELSDAILNGNMPEIAKELGDVLLHIVFYARIGSETNDFDITFVINQLCDKLIRRHPH
                     IYSDTVVKNEKEVKENWEKIKLTEGNRSVLSGVPVSLPAMIKATRIQEKARGVGFDWD
                     EEAQVWEKVQEELQEFKEEAERGDVQKTEEEFGDLLFSLINYARFKNINPEDALEKTN
                     RKFIKRFQYLEESANKAGRKLDEMTLDEMDVYWNEAKKLP"
     gene            6099..7175
                     /locus_tag="PK281_06320"
     CDS             6099..7175
                     /locus_tag="PK281_06320"
                     /inference="COORDINATES: protein motif:HMM:NF020965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HOY48996.1"
                     /translation="MKPILTLCVLFIFTCSLQAQISTYPAAIGFRFHYGFIYQHKKDM
                     DQLVTGHIPAFEVYYRRNFTGKREWEKFYRFPNSGTAIQYIRFNNPMLGNGLAVMPYI
                     SFPILPKSKHSELHFRLASGVGYASKTFDLDKNRKNTVISSHFNAAFNFLLQYHWKPF
                     SGAELLTAISFTHFSNGAYKIPNAGINVVGANVGLNINLGDKVSVNNNSFPILNRDWR
                     YVVSLGGFAKETEPVNRKKFAAFSLSGNALKRISAKSSFGAGLDFMYDATIREKLEQK
                     GDHIDLPARVGVTADYELHVGKVTFPIQQGFYVIDTYKDDPIFYQRIGMRYHMMHNWS
                     LQICLKTHFAKADYAEFGVGYYIR"
     gene            7172..7906
                     /locus_tag="PK281_06325"
     CDS             7172..7906
                     /locus_tag="PK281_06325"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HOY48997.1"
                     /translation="MRTTWLLGFCLVLLFSCKKENMGDCFKSTGVLSIEKRQLPEFDS
                     IYVDRRLQVVLVEDTSYFAVIEAGSHLQELIRTDVENGKLTLRNDNKCNWARSYKNVI
                     KIEVHYTHLRHIVMWGASDITNEDTLKTPNLKVEFRDASGNINLLVNNQNMEVIQHTG
                     AGDVIVRGRTNNLSVYMASLAYADYTGLDAKTVYVENKSAADCYVYSSESFSFRLRGD
                     GNIFYKGLGEVLLSEKTGRGNIVQIN"
     gene            complement(7899..>8529)
                     /locus_tag="PK281_06330"
     CDS             complement(7899..>8529)
                     /locus_tag="PK281_06330"
                     /inference="COORDINATES: protein motif:HMM:NF012509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY48998.1"
                     /translation="NSNLIVKAYHLLHEQFQLPSVNVHLHKLIPMGAGLGGGSSNGAW
                     MLRALNQLFNLNLAIPTLLEYAAKLGSDCPFFIYDSSCKVSGRGEHLEPIELNLSLYY
                     IWLINPRIHISTAQAFSQVKPQSAPLNWKNQIALTPDNWKFSNDFEPGIKQQNPTISE
                     LLQTLNSRGALYSAMTGTGSTVYGIFTHMPDITGIDATYFSKVISAKLS"
BASE COUNT         2566 a         1673 c         1663 g         2627 t
ORIGIN      
        1 caattttctg tacattcttg ttgattttgc tttcgagtaa ttatttgaat gcgcaaaata
       61 cattagtgta taccgaggac tttgaaaatg gcccaggtgt atttgggcta aacacaaccg
      121 gagaaattgg aacgaatgcc ggacaaaaca aatgggttgt aaataacctt tttacgggta
      181 ctccaatcta tcctaataca ataagtcagg atagtacgac gggaggatta atcaattttg
      241 ctccttttag taattattta catattcaag attctgttgc agcatccact caaaatattg
      301 gtaatgctaa ctttcagccc aatgctgcat ccgatcgttt tacctcaaca agtgattttt
      361 gcaccttagg attagatagt gtacgtattg cattttacta tacatgtgaa ggaaatccgg
      421 gtgcaagtgc tcagctttat tatttggcag atggtggagc ttggactcca gttccgggtg
      481 ctgtttttaa taatcaaaca aaatggaact acatcgaatt ttacgatgag ggttttgaca
      541 ataaaaatgc gttaagattt ggttttcgct ggactaacga tggttctggt ggtccaacat
      601 ctggttccat gggagtagat ggagttcgta ttgttggaaa atatgcaccc gatatttaca
      661 atgttcgatt agaattagat agtatttcac ccaatcctgt ttgccggggc gatggtttgc
      721 ttttattttt cagtaaccct gtccctttat gcggaaccgg tttttatgaa gttcaatttt
      781 cgagtgcttt tggcaatttt actaattata catctctagg tatttatcag ttgaataatc
      841 aaaatacaca acaaatactt ttcacgctcc caataccttc caatactaat gctagcccgt
      901 gttataatat tcgggttatg cgagtcgata taacgcctgt aattgtttcc gatacaagta
      961 tttgtattga agtaataaac tgtcccaatc aaattatcac ccttcaacct gcagtattat
     1021 caaatccaac tgatacgcta tgtattggaa gtgtgattga tgttcctttc tattcactag
     1081 gtgtttttca aaacaattta tacgttgctc aattatcgga ttcaaatggt ttgtttcctc
     1141 ccaatccaaa tgttttaggc tttgtaagtg ataatacaac ttatcctcct ggaacgattc
     1201 ccaaaggaaa tgtgcccggc ttagtaagac ctcaaaatca acctattcca ccaggttgta
     1261 attactatat ccgcgttatt tcgagtaatc caaccactat tggtagtgtt tacggtcctt
     1321 tctgtattcg agaatgtgat attgagacga atgaaaaagt tgatgtccag ttttgtattg
     1381 atgatgtaaa tggcggagat actttgctga cggttgatgt aggagttttt cctcctccag
     1441 ctacctattc accacctaac gaatttcaaa ttcaattact cgatttccaa agctttggtg
     1501 tcattaatac tggagttgtt ggttctgtag tagcagttaa cgatacagta gtacaattat
     1561 ccataccgaa tttgccaaac ttatttactg ttggattgat tccaggaaac tactacatgc
     1621 gaatagtagc aaccaattcg gatcaaaact gggatttgtc tggaacctta gtgcgtttaa
     1681 ctatcggtgc ccccaaccct agcccactct ccatcggaat tatcgatatc aatacgtttt
     1741 tgaatgttcc attcgatggc gatacaacca tttgtgtgaa tgaagcaatc tacttcttgc
     1801 taagtcctta caacttttcc tcttcctatg tttggtcgtt aaacaatgat ccgaatttct
     1861 tcgaaggagg accatacaat ccaatattat tcaatggatt aggagattac accttaggtg
     1921 ttacggaaac gaattttgga tgtgttggcc caggttcaaa tgatgccatt attcgtgtac
     1981 aaggatttcc gagtgcatct attgttggtc ctgcacaggt ttgcttaggt gatacagcaa
     2041 cattctcagt tccgttaaat cagaatactt actattcctg gacaaccaat ccgggttcaa
     2101 ttgtagattc attgagtaat actgctagta ttagcttccc atcaagtggt acagcatcca
     2161 tacatatgga agcagttaac gaatgtggtt cacaggaatc aaccaagacg gtgtttgttc
     2221 gtcagccacc acttgtagta acaagtaatg atacttcagt atgtgtggat gcgcaactta
     2281 cattaaacac agctgatggt ataaatttca aattttattg gtcggaagat ggtcagctaa
     2341 tatctcagga tagagagatt accgtgaatc ctgatacaac gacgacttat gttatccgag
     2401 taacgaatta tggaagtcta gcttgtgaat cggccgatac agttactgtt accgtgcaat
     2461 atccggatac tggaagtgtg aaaacgattg atctatgcca aggagaatct gctacacttc
     2521 aatctgattc aactggtata agttatttgt ggagtactgg cttattaact cagtcaatca
     2581 cagtaagtga tagtggttgg tattatgtga tgataaatca ggcaaatgag ttatgtccga
     2641 tgttggattc gtttaaggta aatgtgaaag tatgctatca acccttagaa ttaccgaatg
     2701 tattttcgcc taatggtgat ggaatgaatg atgttttcac acccggacaa acctacatgt
     2761 acgaagattt caacatagtc atatacaacc gctggggctt gaaagtatac gaatcgatga
     2821 atccgttttt tgaatggaat ggtcaggatt taaatgggaa tcaattgacc gatggaactt
     2881 acttctacat cgccaaacta gagcatcacg agaattccga tcttcaaaaa ggaacggtaa
     2941 ctatactcgg taaatagaaa aaagaaaggg ggaaaaaatc cccctttttt attttttcat
     3001 tccagctaaa tatgctgctt taataggaat ctccaagact atttcggatc gataaggcat
     3061 actaccttgg gaagcgggta cgaattctag tttattcaac tcagcaatta actgctcatc
     3121 gataccaaat ccaacttgac cgattgaata tgcctgctgc acttttccat cgaaattaac
     3181 tttgaaacca atcatcgcat tagcgaccaa attcgcatca atagcgcctt gggaaattgt
     3241 taaacgattg tataagaatc gaaataagga atcttctcct tctaagaaga atgcttcgtg
     3301 attagcaata tcaaagttga catcgtaatt ttttcctctt tccaatattt tctgttcctg
     3361 atccgatttc tgagctttaa acccagtact cgattccgtt tgagcaaaca tgaatgtgct
     3421 gctagttatt aatgaaaata atggcaagta ctttaaaaat gaagctttca tggataaacg
     3481 ttagtgaaat taaaacaaaa ttaaaattaa ttagatggca gactgattat cctcaaataa
     3541 agatgggttt attttcctct aactgccttt tcgattatat cccacatagg tgcgggcaca
     3601 tgttctaaat tattgaattc accaccacct tgaagccatt caccaccgtc aattgtcaca
     3661 acttctccat tcataaaatt tgcaaagtca gacacaagaa aagctgcaag attgcctaat
     3721 tcgggatgtt ccccaaaacg tttaagtgga atacgtgcaa gcggatcaat cttcttagct
     3781 aattcttctg gaaacaaacg actccaagca ccttcggtag gaaatggccc gggtgcaatt
     3841 gcattactac gaatgccata tttagcccat tccacagcca aacttcgagt taatgctaat
     3901 actccagctt tagcagtagc tgatgggaca acatatcctg aacctgtcca agcataagtt
     3961 gttacgatat tgagaaaact accttcgatt ttttcagcaa tccaacgctt tccaacagct
     4021 aatgtgcagt tgtatgaacc tttgagaact atatcaacaa tggtatcgaa tgctttgtga
     4081 ctcaagcgtt cggtcggact aataaaattc ccagcagcat tattaaccca tacgtttacg
     4141 ggaccgagtt cagtccaagc tttttcaatt gcagcttcta cttcatccat tttacgcaca
     4201 tcgcacaccg catatcggat aaaaccaccc gtttcctttt ctaattcggc acaggtgttt
     4261 ttaagaactt cttctttccg actggtgatt aatacacgcg cacctaactg gagaaatgtc
     4321 ttggtcatgg atcgacctaa acctgtccca ccacctgtaa cgactatatt tttacctgca
     4381 agtgcattgt cacgcaacat cagggaagtt tctggcttta tcatgattat cttggcatta
     4441 tgtttgaaaa ccaaacctac aaaaatcccc cgttgaccat gatattccga acacttctgg
     4501 ctgctttatt tacacaagct gctttttttt cgtctgccca aatggcttct ctcacgctga
     4561 gcttgcaaga cagcagttta tttacagccg aattaaatgg cacttcctac tcggatgctt
     4621 ctaatgtgat gaatatttca cgtatcactc cgggtccaca aaaactaaaa gtcatcaaac
     4681 tgatgcaaat gggtagttca atcgtgaaaa agcccgttta tgatggaact atcaaccttc
     4741 cggcaggtaa aacaaccgta gcatatatag atcaatacaa ccaatttaga attagttcaa
     4801 ctccacccgt tagttcaaca tcatctggtt catcgaacca aacaagttcc aattcttccc
     4861 cttatttcgt cccagatcca tcagcaataa aagcctatac acagcctaaa acaactgcac
     4921 aaggcatgag caatgatcaa tttgcaggga aaatggaagt gcttagatca atcaaccaag
     4981 aaaatcaacg ctataaaaca gccagagatt tcatcagctt aggaacctat aactcgaatc
     5041 aaattgctga aatgatgttg actttaaaca acgaacacaa ccgagttaat ttagccgata
     5101 tggggtatga atatgctgta gacaaagaaa attatggagt tgtattcaat gcacttcgac
     5161 gaccatcaag tgttaggaaa ttaacccgaa gattaaatta attaacgttc tccaatacaa
     5221 gccacaccat ttacgtgtgg cttttttatt tcaaggaagt ttcttggctt cgttccaata
     5281 cacgtccatt tcatctaaag tcatttcatc caatttgcga ccagctttat tggccgattc
     5341 ttcaagatat tgaaagcgtt taatgaactt acgattggtc ttttctagtg catcctcggg
     5401 attgatattc ttaaatcgag catagttgat taaagaaaaa agaagatcgc caaattcctc
     5461 ttcggttttc tgaacatcgc cccgttcggc ttcttcctta aactcttgta attcttcttg
     5521 caccttttcc caaacctggg cttcttcatc ccaatcgaaa ccaactccac gagccttttc
     5581 ctgaatgcga gttgctttaa tcatggctgg taatgaaaca ggcacaccac tcaaaacaga
     5641 acgattaccc tctgtcaact taattttctc ccagttttct ttgacttctt tttcattctt
     5701 caccaccgta tcgctgtaaa tgtgcgggtg acgacgaatg agcttatcgc agagttgatt
     5761 gatgacgaaa gtgatgtcga aatcgttggt ttcggagcca atacgagcgt aaaaaacgat
     5821 gtgcaataag acatcaccca actcttttgc aatctcgggc atgttaccat tgagaatagc
     5881 atccgacaat tcgtacgttt cttcgatggt taaatgacga aggctttcga gggtttgttt
     5941 tttatcccat ggacattgct cgcgtaattc atccatgatg gtaagcaaac gcgtgaatgc
     6001 ttgttctttt tcaagttgtg tcgacatacg aatttagcct ccgtttttgt ttttacattt
     6061 gcgcaaatat gatgaagcag aaaccggtgc aaaagtacat gaaacccatt ctgacacttt
     6121 gtgttctgtt tatttttacg tgtagccttc aagctcagat aagtacctat cctgcagcga
     6181 ttggatttcg ctttcattat ggctttattt atcagcacaa aaaagacatg gatcagctgg
     6241 taacagggca cattccagca tttgaagttt attatagaag aaactttacg ggtaaacgcg
     6301 aatgggagaa attctatcga tttcctaatt caggaacagc cattcaatac attcgattta
     6361 acaacccaat gcttggaaat ggtttagcag tgatgcctta cattagcttt ccaatcctac
     6421 ctaaatcgaa gcattcggag ttgcattttc gattggcttc gggtgttgga tatgcgagta
     6481 agacttttga tttagataag aatcgaaaga atactgtgat tagttctcac ttcaatgcgg
     6541 cattcaattt cttgcttcaa tatcactgga aacccttttc aggagcagaa ttattgacag
     6601 caatttcttt cacccatttt tccaatggag cctataaaat tcccaatgca ggaatcaatg
     6661 ttgtgggagc taatgtggga ctgaatataa atttgggaga taaggtttct gtgaataata
     6721 actcattccc catcctgaat cgagattggc gctatgtggt tagtttgggt ggatttgcga
     6781 aagaaactga acctgtaaat cggaagaagt ttgcggcatt tagtttatca ggtaatgcat
     6841 tgaaacgtat tagtgctaaa agttcatttg gtgctgggct agatttcatg tatgacgcta
     6901 ccattcgaga aaagctggag caaaagggcg atcatatcga cttgcctgca cgtgtaggtg
     6961 taactgctga ttatgaatta cacgtaggaa aagtcacctt tccgatacaa caaggatttt
     7021 atgtgattga cacgtacaaa gacgatccaa ttttctatca acgaattgga atgcgctacc
     7081 acatgatgca taattggagt ttacagattt gcttaaaaac acactttgct aaagccgact
     7141 acgctgaatt tggagttggg tattacatca gatgagaact acttggttac ttggattttg
     7201 tttggtgttg cttttttcgt gtaagaaaga gaacatgggc gattgcttta aaagtacggg
     7261 tgttctaagt atagaaaaac gtcaactacc cgaatttgac agcatatatg tggacagaag
     7321 gttacaagtt gttttagttg aagacacgag ttattttgct gttattgaag caggaagcca
     7381 tttgcaagaa ttgattcgga cggatgtaga aaatggaaag ttgacacttc gaaacgacaa
     7441 taaatgcaat tgggcaagga gttacaagaa tgtgattaag attgaagttc actacacgca
     7501 tttaaggcat attgtcatgt ggggagcgag tgatatcacg aatgaggata cattgaaaac
     7561 gcccaatttg aaagttgaat ttcgagatgc atctggaaat attaacctac tcgtgaataa
     7621 ccaaaacatg gaagttattc agcatactgg agcgggtgat gtaattgttc gtgggagaac
     7681 caataattta tcggtttaca tggcatcact tgcctatgcc gattatacag gcttggatgc
     7741 taaaactgtt tatgtagaaa ataaaagtgc tgccgattgt tatgtttata gcagcgagag
     7801 tttctccttc cgcctacgcg gagatggaaa tatcttttac aaaggtttag gagaagtgct
     7861 gttatccgaa aaaacgggac gaggcaacat tgttcagatt aactgagctt agccgaaata
     7921 acttttgaaa agtaagtcgc atcaatgcca gttatatcgg gcatgtgagt gaaaatacca
     7981 taaacagttg atccagttcc tgtcatggcc gaatacagag ctccacgcga attcaacgtt
     8041 tgaagcaatt cgctaatggt tggattctgt tgcttaatac ctggctcgaa atcgttgctg
     8101 aatttccagt tatcaggtgt taatgcaatc tgatttttcc agtttagcgg agccgattga
     8161 ggttttactt ggctgaaggc ttgagcagta gaaatatgaa ttcttgggtt aatcagccaa
     8221 atgtaataca agctcaaatt cagttctatg ggttcaagat gttctcccct accacttact
     8281 ttacaagatg aatcatagat aaaaaacgga caatcggagc ccaatttagc agcgtattca
     8341 agcagggttg ggattgctag attaagattg aataattgat tgagagcacg taacatccaa
     8401 gcaccattag aggaaccgcc acccaaacct gcacccatcg gaattaattt gtgcagatga
     8461 acatttacag atggtaactg aaactgctca tgtagcaaat gatacgcttt aacgatcaaa
     8521 ttggagttc
//
LOCUS       DAOOQW010000085         8527 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_10272_length_8527_cov_4.334042, whole genome shotgun sequence.
ACCESSION   DAOOQW010000085 DAOOQW010000000
VERSION     DAOOQW010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 8527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8527
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10272_length_8527_cov_4.334042"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /gene="apaG"
                     /locus_tag="PK281_06335"
     CDS             complement(<1..367)
                     /gene="apaG"
                     /locus_tag="PK281_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009187262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HOY48999.1"
                     /translation="MITLVTNDIRVTVETKFQELYSDPSKMYYLFTYRIKIENTGDYD
                     VQLLRRHWNIVDSISEHRDVDGAGVVGQQPVLRPGEVYEYESACNLKSEFGKMFGNYT
                     FERLVDGEAFQVSIPEFKMS"
     gene            complement(540..1835)
                     /locus_tag="PK281_06340"
     CDS             complement(540..1835)
                     /locus_tag="PK281_06340"
                     /inference="COORDINATES: protein motif:HMM:NF028426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5103 domain-containing protein"
                     /protein_id="HOY49000.1"
                     /translation="MLRFNCVLFSCLFSLISFAQDDPDYYTDNFLRFEDHTYRPTIKT
                     VILQQLGTDGSLPLIELGSNTQLQLQFDDLDPVQRQLYYTLEHCNADWTPSNVMKSRS
                     IQGLQQDLVQDFRYSFNTRQQYLHYNLVFPNENMKIILSGNYLLKVYEDGDPENLVLT
                     RRFMVYTTKAKVAARVKRPTLMNLRETHQEIDAVVDVSAIPITNASSSVKLVIKQNNR
                     WDNAVWLKPFSVNSSSINYDYDDGSNCFAGGNEYRWVDIRSLRMNSDRVKRIIRDSVL
                     VETQLLDDPIRAFSTYRSLPEANGKFFIRNQEGSEPEVDGDYTWVDFRLPFEAPVVNG
                     NLFLFGSLCDWQFKKPFQPVYDYPDKAYKARILLKQGIYNYSYVFQSSKGGSGDESYI
                     EGNFFNTENDYQILFYYRPYTEVHDELIGIGQINSIRNQ"
     gene            1902..4028
                     /locus_tag="PK281_06345"
     CDS             1902..4028
                     /locus_tag="PK281_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013621296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HOY49001.1"
                     /translation="MKMLEQLAIQTGIANKQIEKVLELFDNGSTIPFIARYRKEMTGG
                     LDEVQLEAIQDARKHWLKLDERRKSMLDSLEERGILTPELKAQLLQASTIQDAEDIYL
                     PYRPKRRTKATIARENGLEPLAKMIMSQGNDDPFRMAGRFLNAAIVDEDSALQGARDI
                     IAEWVSDNAGARQTLRVQFERFAVLSSKVLKGKEEEGSKFRDYFGFSEQLSRIPSHRF
                     MAVMRGQAEGILSISALPEEEKALEKLNRIFVKSRSEASQQVEKAVKDSYKRLIAPSL
                     ENEVITIIKEKADKQAVQIFSENLRQLLMGAPLGEKRVLGIDPGFRTGCKLVCLDDNG
                     GLLHNETIYPHAPQMQDKQAMAKISQLVATYKIDAIAIGNGTAGRETEQLIQRMRFEK
                     PVQVFVVSESGASVYSASKIAREEFPQYDVTVRGAVSIGRRLQDPLAELVKIDPKSIG
                     VGQYQHDVDQKLLEDRLTNVVLSCVNQVGVNLNTASAHLLTYISGLGPSLASNIVDYR
                     NTHGPFSKRSDLKKVPRLGDKAFEQSAGFLRIDTSDNPLDNSAVHPERYSLVAQMAKD
                     SGLSLKDIVGKKDLRQMIKPERYLTQDVGLPTLEDILSELEKPGRDPRKQAKVLVFDP
                     SIRKPEDLIPGMVLPGIVTNITAFGAFVDVGVKQDGLVHVSQLADRFVSDPMEIVKLH
                     QHVQVRVMEVDLARKRIAFSMKGINS"
     gene            4080..4529
                     /locus_tag="PK281_06350"
     CDS             4080..4529
                     /locus_tag="PK281_06350"
                     /inference="COORDINATES: protein motif:HMM:NF021983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2452 domain-containing protein"
                     /protein_id="HOY49002.1"
                     /translation="MQEEEPFVNPIDPDKVAQNPGLLPYAHTLSSGVIKPDDLGKIKS
                     NALLAMEQQTEMQLNQLQQQIQLLYNQAQEIKNRTDISVWIYQSVMGFDPIVNHVYHL
                     YEKEDGQHFLSMVAPNEWGRTKKFTRFIATVRLLADHTWDVLQKGDV"
     gene            complement(4573..6120)
                     /locus_tag="PK281_06355"
     CDS             complement(4573..6120)
                     /locus_tag="PK281_06355"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOY49003.1"
                     /translation="MKKTIILFSLSALILSVGCSRKEEQIALTEYSSGPILKQKSNPV
                     NAPEGEAFSKSKLDAFVLQTLESQNDFRWEKMDLKTLWSAAQYGDQSVAIGYKPSNTG
                     DISQSIHTIDIQSKPWKDVHDALIETILNELNKTAINPVKWTDILIEDDQTLPILTIR
                     LTDKNTFTLLYNLENVRYIEPLDYWPDNVERSSSGCSAATASVNASDWTTLTPGCKLP
                     WNYNNVNIPNAWNIAQGQGVTVGIIDAGISSSQFFLSTLFSSGYSNNGRTLTTDYTYG
                     TGAYTSCTHGTSMSGLAVGPRNSGNATTGVAYKSNLHFIRACEDVVLDASSEKTAVKN
                     ALIKMGNKTDVKIVSMSIGTPFSSSVLLDGVNYANNKGKLIFAAAGTSFSWTSWWGVI
                     YPAAYSACVAVTGVQENGNTCETCHDGSEVMFTIPMERNSNTDRNSLSLPATGSNPTY
                     IGGSSCATAMSAGIAALVWSAKPSLTKNQVFTILRNTSQNYPSFDNDHGYGNLNAGAA
                     VNSALSY"
     gene            6344..7960
                     /locus_tag="PK281_06360"
     CDS             6344..7960
                     /locus_tag="PK281_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015782931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOY49004.1"
                     /translation="MIAVSNISLRYGKRTLFEDVNLKFIPGNCYGIIGANGAGKSTFL
                     KILSGDIDANTGEIAITPGERMAVLRQNHYEFDEYQVLQTVMMGHKKLMDIIQEKDAI
                     YAKTDFTDADGIRASELEGEFAEMEGWNAESDAANLLSGLGVSEDLHYRLVSELNGTQ
                     KVRVLLAQALYGNPDILLLDEPTNDLDVHTIAWLEDYLADFKNTVIVVSHDRHFLDAV
                     CTHIVDIDYSKMRLYTGNYSFWYESSQLISRQMADKNSKMEDKRKQLQEFIARFSANA
                     SKSRQATSRKKLLDKLNVEDLQASNRKYPAIIFKPEREPGDQILHVENLSASLNGKQL
                     FSNVTFNMHREDKIAVVAQDHQAVSFLFDILVGDRKPDSGTVTWGTTIKPAYLPGDNS
                     TYFTSDLNLIDWLRQYSTDPDETFVRGFLGKMLFSGEETLKKCSVLSGGEKMRCMISR
                     MMLSGGNLLILDEPTNHLDLESITAFNNSITDVKTPVLLSSHDHQFMQTTANRVIELL
                     PKGIIDRQLTYDEFLENEAVLNRRTELQPK"
     gene            8033..>8527
                     /locus_tag="PK281_06365"
     CDS             8033..>8527
                     /locus_tag="PK281_06365"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOY49005.1"
                     /translation="MSILIIEDEPKLAEFIRQGLEENGYSTEIALDGKLGLAKALESN
                     FDLVILDVIIPEVSGIDVCKGIRAEKPNMPVIMLTALDTLSDKQRGFDAGADDYLVKP
                     FEFRELLMRVKALTRRVSVSSLSSHVLKVKDLELDLDLKLARRGGKEIALTAKEYQLL
                     EFLMR"
BASE COUNT         2673 a         1545 c         1718 g         2591 t
ORIGIN      
        1 ccgacatttt gaattcaggg atgctaactt ggaatgcttc tccgtcaact aaacgttcaa
       61 atgtgtagtt accgaacatt tttccaaatt ctgatttcaa attgcaagca gattcatatt
      121 cataaacctc tcctggacga agaaccggct gttgaccaac aacaccagca ccatctacat
      181 cacgatgttc agagattgaa tcaacgatat tccagtgacg acgtaacaac tgtacatcat
      241 aatctccggt attctcgatt ttgatcctgt atgtgaagag gtaatacatc ttcgaaggat
      301 ctgaatacag ttcttgaaat tttgtttcaa ctgtaacccg aatgtcattt gtaactagtg
      361 taatcatggc ttgagtgttt tgttgagaca atgttaggca caaatcatca tacaaaacaa
      421 attgacatcc tctggaggcc ttgataggcg cgggttttag atattggttg tgtcaaaaac
      481 agatttgact ttacaaatat gcaaaaaaat cttacatcca aattacattg tttttagaat
      541 tattgatttc ggatcgaatt gatctgtcca attccaatta attcatcatg tacttctgtg
      601 taaggcctgt aatagaagag aatctgataa tcattctcgg tattaaagaa gttgccttct
      661 atgtaggatt cgtctccaga accacccttg gacgattgaa aaacataact gtagttgtat
      721 attccttgct taagtaagat acgcgccttg tatgctttat cggggtaatc gtaaacaggt
      781 tgaaaaggct ttttgaattg ccaatcgcat aacgaaccga ataaaaaaag gttaccattt
      841 acaacaggag cttcgaaagg aagtctgaaa tcgacccaag tataatctcc atctacctca
      901 ggttcagagc cttcttgatt tctgatgaag aattttccat tagcttcagg taaagatcga
      961 taggttgaaa atgcacggat aggatcatcg agtaattgtg tttcaaccaa aacagagtca
     1021 cgaataatcc tctttacccg atcactattc attcttaggc ttcttatatc aacccagcgg
     1081 tattcatttc cacccgcaaa acaattactt ccatcatcgt aatcgtaatt tatagatgag
     1141 ctattcactg aaaacggctt caaccacaca gcattatccc atctattatt ctgtttaatt
     1201 accaacttaa ctgaagaaga cgcatttgta ataggtatag cactaacgtc tacaacagca
     1261 tcaatttcct ggtgtgtttc tctcaaattc attaaagtag ggcgttttac acgcgctgca
     1321 actttagctt ttgtcgtata taccatgaag cgtcttgtaa gaaccagatt ttcaggatca
     1381 ccatcttcat ataccttaag cagatagttt ccacttaata taatcttcat attctcattc
     1441 ggaaacacga gattataatg gagatactgt tgacgtgtat taaatgagta gcgaaaatcc
     1501 tgaaccaaat cttgttgtaa tccttgaatc gatctcgatt tcatcacatt agatggtgtc
     1561 caatcagcat tgcaatgttc gagtgtataa tatagttgtc gttgtacagg atctaaatca
     1621 tcaaactgta attgaagttg ggtgtttgaa cctaattcaa taagtggaag tgaaccatct
     1681 gttcctaatt gttgaagtat tactgttttg atggtcggtc tataggtatg atcttcaaat
     1741 cgtaggaaat tatctgtgta atagtcagga tcatcttggg caaaagagat taaggagaat
     1801 aagcagctga agagaacaca attaaatcga agcataaaga atcatttctg cgaagataca
     1861 ttgcatgcca acaacacaat ctgaatatgc atatttgtag gatgaaaatg ttggaacagc
     1921 tagctataca aacaggaatt gccaataagc aaatcgaaaa agtgcttgag ctttttgata
     1981 acggaagcac aatccctttc attgctcgat atcggaaaga gatgacgggt ggattagatg
     2041 aagttcaatt ggaagcaatt caagatgcta gaaaacattg gttaaaactc gatgaacgta
     2101 gaaaatcgat gcttgattca ttagaagagc gtggaatatt gactccagaa ttaaaagcgc
     2161 aattacttca agcatcaacc attcaagatg cggaagatat ttatttaccc tatcgcccta
     2221 agcgtcgcac taaagctaca atagcacgag aaaatggatt agagcctttg gctaaaatga
     2281 tcatgtctca agggaatgat gatccattcc gaatggcagg tcgttttctc aatgctgcta
     2341 ttgttgatga agattctgcc ttacaaggtg cgagggacat tattgctgaa tgggttagcg
     2401 ataacgccgg agcccgtcaa acacttcgtg ttcaatttga acgatttgct gttttatcaa
     2461 gtaaagtgtt aaaagggaaa gaagaagagg gaagtaaatt cagagattac tttggttttt
     2521 cagagcaact ttcacgaata ccatctcatc gatttatggc tgtgatgcgc ggtcaagccg
     2581 aagggattct tagtatttct gctttgccag aggaagaaaa agccttagaa aagctcaatc
     2641 ggatttttgt aaagtctcgg agtgaagctt cgcaacaagt tgaaaaggca gttaaggata
     2701 gctataaacg cttaatcgct ccttctcttg aaaatgaagt gataacaata ataaaagaga
     2761 aagctgataa acaagccgtt cagattttct cagagaacct tcgtcaatta ctgatgggag
     2821 ctcctttggg agagaagcgt gttttaggga ttgatcctgg gtttcgtact ggttgtaaat
     2881 tggtttgttt agatgataat ggtggattat tacataacga aacaatctat cctcatgcgc
     2941 cgcaaatgca ggataaacaa gccatggcta agatttctca attagttgca acctataaaa
     3001 tagatgcaat tgcaattgga aatggtaccg caggtaggga aactgaacaa ctaattcaac
     3061 gcatgcgttt tgaaaagcct gtacaagtgt ttgttgttag tgaaagtggt gcatctgttt
     3121 attccgcatc caagattgcg cgagaggagt ttcctcaata tgacgtaacg gttagaggtg
     3181 cggttagcat tggtcgtcgt ttgcaagatc cattagcaga gttagttaag attgatccaa
     3241 aaagcattgg agtggggcaa tatcagcatg atgtggatca gaaattattg gaagatcgat
     3301 taacaaacgt agttttaagt tgtgtgaatc aggtaggtgt taatttgaat accgcaagtg
     3361 ctcatttgct tacctatata tcaggtttgg gaccttcact tgcatctaac attgtagatt
     3421 atcgaaatac tcatggtcca tttagtaagc gttcagattt gaagaaagtt cctcgtttgg
     3481 gtgataaagc ttttgaacaa agtgctggat ttttacgaat cgatacaagt gataatccct
     3541 tagacaatag tgccgtgcat cccgaacgat attctttggt agcacaaatg gctaaagatt
     3601 cgggattgag tttgaaggat attgtaggta aaaaagatct ccgacaaatg atcaaacctg
     3661 aacgctacct tactcaagat gtgggtttgc caactttaga ggatatatta tctgaattag
     3721 agaaacctgg tcgcgatcca cgtaaacaag caaaagtttt ggtattcgat ccatccatcc
     3781 gcaaacccga agatttaatt cctggtatgg tacttcccgg catcgttact aatataacag
     3841 ctttcggtgc ttttgtagat gttggagtta agcaagatgg attagttcac gtgtctcaat
     3901 tggctgatcg attcgttagt gatccaatgg aaatcgttaa attacatcaa catgtacaag
     3961 ttcgtgtaat ggaagtagat ctagctagaa agcgaatcgc attttctatg aaaggaatca
     4021 attcctgata tcttaatcca ttttagaaca aaatcaatta aatcgcgttc gtagcaacca
     4081 tgcaggaaga agaaccattt gtaaatccaa tcgaccccga taaagttgcc caaaatccag
     4141 gacttttacc ttatgctcat actttgagca gcggtgttat aaagcctgat gatttaggaa
     4201 agattaagag taatgctttg ctagctatgg agcagcaaac tgaaatgcag ctaaatcaat
     4261 tgcagcaaca aattcaattg ctttataatc aagcacaaga aattaagaat cgaacagata
     4321 tttcagtttg gatttaccaa tctgttatgg gatttgatcc tatcgtaaat cacgtgtatc
     4381 acttatatga aaaggaagac ggacaacatt ttctttccat ggtagctcct aatgaatggg
     4441 gaagaaccaa gaaattcaca cgttttattg ctactgtaag actattggcc gatcatacct
     4501 gggatgtact tcaaaaaggc gacgtttaaa aataaaggga tgcagttttt tttctgcatc
     4561 cctttttgtt tttcaatagc taagcgccga attaactgca gcaccagcat ttaaattccc
     4621 ataaccatga tcattatcaa atgacggata gttttgagat gtatttctta gaattgtgaa
     4681 tacttgattt tttgttaagc ttggttttgc actccaaact aacgcagcaa ttccggccga
     4741 catagctgtc gcacaagagc ttcctcctat gtaagtagga ttagatcctg ttgctggtaa
     4801 tgaaagtgaa tttcgatctg tgttactatt tctttccata ggaatcgtaa acataacttc
     4861 actaccatca tgacacgttt cacacgtatt accattttcc tgaactcctg taacagctac
     4921 acacgctgaa tatgctgcag gataaattac tccccaccaa ctagtccaac taaatgaagt
     4981 gccggctgct gcaaaaatca atttgccttt gttattggca taattcaccc catcaagtaa
     5041 tacactgctc gaaaagggag tcccgatact catactcaca attttcacat ctgttttatt
     5101 tcccattttt atcaaagcat tcttaactgc agttttttct gacgaagcat caagaacaac
     5161 atcttcacac gcacggataa aatgcaaatt agatttataa gcaacgcctg ttgttgcatt
     5221 tcctgaattc ctaggtccaa cggctaatcc gctcatactt gttccatgcg tacaacttgt
     5281 ataagcgcct gtgccataag tataatcagt tgttagtgtt cgaccattgt tggaatatcc
     5341 actagaaaac aaggtactta agaagaactg ggaagaactt attccagcat cgataattcc
     5401 aacagtaact ccttgccctt gtgcaatatt ccaggcgttg ggaatattca cattgttata
     5461 gttccaagga agtttgcagc ctggagttag agttgtccaa tctgaagcat ttacagaagc
     5521 tgtagcagca ctgcaacccg aagatgaacg ttctacattg tcgggccagt aatccaaagg
     5581 ctctatgtaa cgcacattct ccaagttata taacaaggtg aaggtatttt tatccgttaa
     5641 gcgaatcgtt aaaattggaa gtgtttgatc gtcttcgatt aaaatatcag tccattttac
     5701 tgggttgata gctgttttat tgagttcatt taagatcgtt tcaattaaag catcatgaac
     5761 atctttccac ggtttacttt gaatgtcgat tgtatgaatc gattgtgaaa tgtcaccagt
     5821 gttgcttggt ttatatccaa tagcaaccga ttgatcacca tattgagcag cactccaaag
     5881 tgtttttaag tccatttttt cccaacgaaa atcattttgt gattcaagtg tttgaagaac
     5941 aaaggcatct agttttgatt tggagaatgc ctcaccttcg ggcgcattaa ctggattaga
     6001 tttttgtttc aatatagggc ctgaagaata ctcagtaagg gcaatttgct cttctttacg
     6061 cgaacatcca acgcttaaaa tgagtgcact tagactaaaa aggataatag tttttttcat
     6121 tagtattaat ttttgaatga gagcatgaaa ctacaattct tagattttca aattcaagtc
     6181 gatggaaacg aatcattaac aagcctgtta ataagcttgt tgaaatgctg agataaactc
     6241 attaacgaat tttacagctt gagaaattac ttatagcaat ttagatttca agctcaaaat
     6301 tgaagttgaa atagattacc tttgcacgcc ttttttagcc cctatgatag ctgtttcaaa
     6361 tatctctctc cgttacggaa aacgtactct ctttgaagac gttaatctca aattcattcc
     6421 aggaaactgt tatggaatta ttggtgctaa tggagccgga aagtcgactt tcttgaagat
     6481 actctcagga gatatcgatg ctaatacagg tgaaatagcc ataacaccgg gagaacgtat
     6541 ggcagttctt cgtcagaatc actatgaatt tgatgagtat caggttcttc aaacggtgat
     6601 gatggggcac aaaaaactca tggatattat tcaggagaaa gatgccatct atgctaaaac
     6661 cgattttact gatgcagatg gtattcgtgc atctgaactg gaaggcgaat ttgccgaaat
     6721 ggaaggctgg aatgcagaaa gcgatgctgc aaacttactt agtggtttag gtgtttcgga
     6781 agatctccat tatcgtttag tttcagaatt aaatggaact caaaaagtac gtgtgttgct
     6841 tgctcaagcc ttatacggaa atccagatat ccttctactc gatgagccta ccaacgacct
     6901 agatgttcat accattgctt ggttagagga ttacttagct gatttcaaaa acacagttat
     6961 tgtagtttcc cacgaccggc actttttgga tgcagtttgt acgcatattg tagacatcga
     7021 ttatagtaaa atgaggcttt ataccgggaa ctactctttc tggtatgaat caagtcaatt
     7081 gattagtcgt caaatggcag ataagaattc caaaatggaa gataagcgta agcaacttca
     7141 ggaattcatc gctcgattta gcgcaaatgc ttcaaaatct cgtcaggcaa ctagccgaaa
     7201 gaagttattg gataagctta atgtagagga tttacaagcg tcaaatcgta agtatccagc
     7261 tatcattttc aaacctgaac gtgaaccggg cgaccagatt cttcatgtgg agaacctgag
     7321 tgctagtttg aatgggaaac aactattctc caatgtgaca tttaatatgc accgggagga
     7381 taaaattgct gtagtagctc aagatcatca agctgtttcg tttttattcg acatacttgt
     7441 tggagatcgt aaacctgact ctggcactgt aacatgggga actactatta agccagctta
     7501 tttgccgggt gataatagta catactttac ctccgatttg aatttgatcg attggttacg
     7561 tcaatattct accgatccag atgaaacatt tgttcgtgga tttttgggga aaatgttgtt
     7621 ttctggagaa gagactttga aaaaatgctc tgtcctttcg ggtggtgaaa aaatgcgttg
     7681 tatgatttct cgaatgatgc taagtggtgg gaatttgttg attttagatg aacctactaa
     7741 tcacctcgat ttggaatcaa tcacggcatt caacaattcg ataacagacg taaaaacacc
     7801 tgtattatta agttcgcatg accaccagtt tatgcaaact actgctaatc gcgtaatcga
     7861 actcttgcct aaaggaatta ttgatcgtca acttacgtat gatgaatttc ttgaaaatga
     7921 agcagttcta aatcgtcgaa ccgaacttca accgaagtaa atcgaatcat taacaggaat
     7981 ttaatagaca agttggactt gaagggctaa tattgtccta tatacttcct gaatgagcat
     8041 tcttataata gaagatgagc ccaagctagc agagtttatc cgccagggac tcgaagagaa
     8101 cggatattcg acagaaattg ccttagatgg aaagcttgga ttagccaagg cacttgaatc
     8161 gaattttgat ttggtaatat tggatgtaat tatccctgaa gtcagcggga tagacgtgtg
     8221 taaagggatt cgtgcggaga aacctaacat gccagttatt atgttaaccg ctttagatac
     8281 cttgagtgat aagcaacgtg gtttcgatgc aggggctgat gattatcttg ttaagccgtt
     8341 tgaattcaga gaattactca tgcgtgtgaa agctttaaca aggcgagtat ctgtttcatc
     8401 gttgagtagt catgtactta aggtaaaaga cttagaactc gatttagacc tgaaattagc
     8461 tagaagagga gggaaggaga tagcattaac agctaaggaa tatcagttac tggagtttct
     8521 gatgagg
//
LOCUS       DAOOQW010000086         8500 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_10306_length_8500_cov_4.661338, whole genome shotgun sequence.
ACCESSION   DAOOQW010000086 DAOOQW010000000
VERSION     DAOOQW010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 8500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8500
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10306_length_8500_cov_4.661338"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            16..747
                     /locus_tag="PK281_06370"
     CDS             16..747
                     /locus_tag="PK281_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007095497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49006.1"
                     /translation="MCFPASLLAQNRIIHAVVALCDNQYQGIVKVPAGIGNGQSPSTN
                     LYWGARYGVKTVFTRSKEWILLGESVQKNPKILQRLVFKHKTAPVIFIADAWDGKYIK
                     ECTEWFIESNSGKHRDSVDVKGKKYPSGGSSSLLCYIGHDGLMDFDLNMNITADGTYR
                     ESIVLACYSKHYFSPWLKKCGAVPLVWSTGLMAPEAYILEAALHEWVSKFNDKEAIRS
                     AAAQAYSTYQKCSLSAARRLLVTGY"
     gene            complement(734..2455)
                     /locus_tag="PK281_06375"
     CDS             complement(734..2455)
                     /locus_tag="PK281_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HOY49007.1"
                     /translation="MSSIDWIVLVLTLSVIIVYGLYRSRTTKDLDGYFRSNQSMPWFL
                     VLLSIMGTQASAITFLSAPGQAYTDGMRFVQYYFGLPLAMIVICIAFVPVFKKLNVYT
                     AYEFLENRFDLRTRTLTSILFLISRGLSTGISVFAPSIILSSLFGWNIYFTNVVMGGM
                     LIIYTVSGGARAVAYTQQLQFIIIFIGMFLAGYLVIQMLPDSVGFVDALHVSGKLGKL
                     NVITTGMKDGSFDWSDKYNIWSGLIGGFFLALSYFGTDQSQVGRYLTAKSLKESRVGL
                     LMNGLVKIPMQFLILLIGVLVFCFYSFFQAPVFFNSVEMKKLEKSAIYSDSLQVLKQD
                     FNQLNLQKQAAVAHYVQLNNGAENTSEMDAQILEMRRLDAASNVLRKKVKTWIDSPSI
                     KGDSNDTNYIFLRFVIDFLPKGLVGLLIAIIFLAAWGSIAAALNSLASSSIIDLHKRH
                     SSVQLTKDQEYRLSRWYTFGWGVFCIAVACFAYNLGNSLIEVVNILGSIFYGPILGIF
                     LCAFWMKSCSAKAVFWSALLSQTVVIILFKTTSISFLWYNVIGCLILMLAAWILSHSI
                     FKKLNNL"
     gene            complement(2567..5053)
                     /locus_tag="PK281_06380"
     CDS             complement(2567..5053)
                     /locus_tag="PK281_06380"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOY49008.1"
                     /translation="MKQLVLLNLFCFFFGSIQSQSAPGQIISQLHHLNSQGRVLYIAA
                     HPDDENTRLLSWLANERHYRTAYLSLTRGDGGQNLIGNEQSEELGLIRTQELIAARNT
                     DGAEQFFTRAFDFGFSKNPEETFRIWDKEKILSDVVLVIRKFKPDVIIARFPTTGEGG
                     HGHHTASAILAGEAFEAAADEKRFPESAKLYGTWKTKSLYWNNFRKWRDPKADVSSAL
                     KLDIGSYLPLLGESIGEISAKSRSQHKSQGFGVALQRGEILEYFDHLAGEKAQTEPFE
                     NLKQGWKNKAISDSIDSIIQQFKIENPASSIPALTRLYQAFQKLSPLENSDHHVQLLQ
                     DIILQCAGFYGEFKANKFAVNPGEAVPVSFSCINRSNQEIKLLSTRAILPNSILVECN
                     KVLTNNEVYTRNDTLKLPAQLAISNPYWLNSPHAEGLFTVNELSQIGKPESEAPIQIE
                     TEWQIGTIHFLKRFPLNYLWVDPVKGELTRRVEVLPAITATVNEDVFILNQSDHRTVE
                     ITIETSANSANIDVTLDLPAYFHSSPSKISVQLGNGAPRKQIIKFDLSANPSRETKQD
                     AVFTIGVMMMEGTNKKELMHISRIDYDHIPIQTWIKPAAFKAVYVNLNRVGENIVYIP
                     GADDKVGDCLKQAGYTVNTIASGDVTTEKLKNVDAVIIGIRAFNTNENMSSLMPILLA
                     YVQQGGTLIEQYNTKNWISDVSVQPGPFPFEVSRERVTDENAPMKRLITDHPIFNTPN
                     QITDQDFTGWIQERGLYFPEKWDSHYTALLECNDPGEKPLQGALIVTEYGKGKFIYTG
                     LSFFRELPAGVPGAYRLFANMIAWGKNDGN"
     gene            complement(5078..5743)
                     /gene="mqnB"
                     /locus_tag="PK281_06385"
     CDS             complement(5078..5743)
                     /gene="mqnB"
                     /locus_tag="PK281_06385"
                     /EC_number="3.2.2.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR03664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="futalosine hydrolase"
                     /protein_id="HOY49009.1"
                     /translation="MKILLIAATQAEIAPFYKNLNFLEEEVNILGDFQLKVLITGAGM
                     VATTYQLTKSLHYFRPDLLLNVGIAGSIDPLILRKSLVNVTSDHFFAFGAEHHDTFLP
                     ASQIGLQPEQFDILQSIPFPENYSKLIEKFRSIQAVKGITVQTVHGEEQSILKMKRLN
                     PEAQIESMEGAAVFYVGLQEQIPVVQIRSISNPVEPRNREGWDLEGSIAVLNDFLIHL
                     FKS"
     gene            5864..6973
                     /locus_tag="PK281_06390"
     CDS             5864..6973
                     /locus_tag="PK281_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49010.1"
                     /translation="MLETSLSRIQWTFRVLLMAMLGTWLLSNKVWMTERNFPVIPIFD
                     GIPSLPSPVDYIFYSILIVLAIYQIIYLENRNTKIALVVLLGFLMIGDQNRWQPYNVQ
                     YFLMISALFFYPKFTDGILNVFRMMVIVFFIFSGIQEINTVFIESIFPWMIGPIASLF
                     PASVEPNILGGGYIFPFLSIAAGVGLLFQQTRDYAVYLGILIHLFYFYALSPMGNDWN
                     HAILPYNLCMAFFTYVLFFSAEFKLKDIFWSKTFRYQQAVVIIFAVLPLLSFFGIYDR
                     MQSFNTYSGKSLYAKIYVTESLVELLPDGVKRYVYRPQQGEPYIETTYWSADALKVSP
                     YSEERVYKKLHEYVCSFSEGDCSARLEIYSYGGGH"
     gene            7007..8278
                     /locus_tag="PK281_06395"
     CDS             7007..8278
                     /locus_tag="PK281_06395"
                     /inference="COORDINATES: protein motif:HMM:NF013395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HOY49011.1"
                     /translation="MVLRILSLSFLFILTQTGVKSQENLSKRIDQYENQLKSLQADQE
                     KVLQELEDLKLENLRSDLQKNGLPSLLPGEEVIMHAAMALVYNEEYEQASWVAHIILP
                     DVVSGMAGRSNDFRPDPLIKTGSAVERDYLIKDTLANGTYKYDGFGFDRGHLAPSADF
                     RWSKKALSESFYYSNMSPQVADFNRDSWAKLEDLLRAYVQEKKVPLYVLTAPLLRPNL
                     PKIERGVNHVAIPVYYYKIALDPVNKQAIAFLMPNKKCEYPTEHYAVSIDSLEHLTGR
                     DFYSSLPDSLENELEKMDSAKPWMKLKEKNDEKPLDPTQLPKKHFNTVQAKLYQNQNE
                     IITVCGTVVSTKLSSKGNVFLNLDKGFPNQIFTVTIFKDQLVNFTYAPQVELMGKVIC
                     VEGKVADFNGTPSMVIEHEKSVRLYLEDDER"
     gene            8265..>8500
                     /locus_tag="PK281_06400"
     CDS             8265..>8500
                     /locus_tag="PK281_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49012.1"
                     /translation="MTNGKCFQFPDKNHCQKRIAALLLKEKIMNLHIEKHMTLAEVQA
                     AFNHSFPFLKIGFFIDKNHDNTLTADEQIHNLKMT"
BASE COUNT         2627 a         1592 c         1647 g         2634 t
ORIGIN      
        1 tgcattctca ttgggatgtg ctttccggct tctttattgg cacaaaaccg aataatacat
       61 gctgtggtag ccttgtgcga taatcaatat caaggaattg taaaagttcc tgctggaatt
      121 ggcaacggac aaagtccttc aacgaattta tattggggag ctcgttatgg tgtaaagact
      181 gtatttacgc gcagcaaaga atggattttg ctaggtgaat cggtacagaa aaaccctaag
      241 attcttcaac gattagtttt taagcacaaa actgcaccag ttatatttat tgctgatgct
      301 tgggatggaa agtacattaa ggaatgtact gaatggttta tcgaatcgaa ttccggaaaa
      361 caccgcgata gtgttgatgt aaaaggaaag aaatatccat caggtggttc gtccagtttg
      421 ctttgttaca taggacatga tggtttgatg gatttcgatc tcaacatgaa tataacggct
      481 gatggaactt atcgggaaag cattgtttta gcctgttaca gcaaacatta cttcagtccc
      541 tggttgaaga aatgtggagc ggttcctttg gtttggtcaa ccggtttaat ggcgccagaa
      601 gcatatatac ttgaagccgc tttacatgaa tgggtgagta aattcaatga taaagaagct
      661 atacgttcgg cagctgcaca agcatattcg acgtatcaga aatgttcctt atcagccgcc
      721 agacgattat tggttacagg ttattgagct ttttgaaaat gctatgtgag agaatccatg
      781 cagccaacat caagattaag cagccaatta cattatacca taaaaagctg atactggtgg
      841 ttttgaaaag gataatcact actgtttgag aaagcaaagc cgaccagaat acagcttttg
      901 cagaacagct tttcatccaa aaagcacaca ggaatattcc gagaattgga ccgtaaaaga
      961 tagagccgag aatattgaca acttcaatca ggctattccc gagattatag gcaaaacaag
     1021 caactgcgat acagaaaact ccccagccga atgtgtacca acgagacaaa cggtattctt
     1081 gatcttttgt cagttgaaca gaactatgtc gtttgtgtaa atcgatgatg ctagaagatg
     1141 ctaatgaatt taatgctgca gctattgaac cccaagctgc aagaaaaatg attgcaatca
     1201 gtaaaccgac aagaccttta ggcagaaaat caatgacgaa tcgaaggaag atatagttgg
     1261 tatcgttgga atcaccttta atacttggag aatcaatcca ggtttttact tttttacgaa
     1321 gcacattact agctgcatct aaacgacgca tttctagaat ttgagcatcc atttcactcg
     1381 tattttcagc tccattattg agttggacat aatgagcaac agcagcttgt ttttgaagat
     1441 ttagttgatt gaaatcctgt tttaatactt gcaatgaatc ggaataaata gctgattttt
     1501 ccagcttttt catttcaacc gaattgaaaa aaacaggggc ttgaaaaaag ctataaaaac
     1561 aaaacaccaa gaccccaatc aacaggatta aaaactgcat cgggattttc actaaaccat
     1621 tcatcaacag ccctacccga ctttctttca aggattttgc tgtgagatat cttcccactt
     1681 gagattgatc cgttccgaaa taagaaagtg ctagaaaaaa tcctccaatt aatccactcc
     1741 aaatattgta tttatcactc caatcgaagc ttccatcctt cataccggtg gtaattacat
     1801 tgagttttcc caattttcca ctaacatgaa gcgcatcaac aaaaccaacc gaatcgggaa
     1861 gcatctgaat gactaaatat cccgccagga acatcccaat aaaaatgata ataaattgaa
     1921 gctgctgagt ataagcaaca gctctagctc caccactgac cgtataaatg ataagcattc
     1981 cgcccatcac cacattggtg aaataaatat tccatccaaa aagcgaggat aaaatgatgg
     2041 atggagcaaa aacgctgata ccagtagaaa gtccgcggga tattagaaat aaaatagatg
     2101 ttagcgttcg ggttcgtaaa tcgaatctat tttctaagaa ttcgtatgct gtgtatacat
     2161 tgagtttctt aaaaaccggg acaaatgcaa tgcagattac aatcatcgcc aagggcaaac
     2221 caaagtaata ctgcacaaaa cgcatcccat cggtataagc ttgtccggga gctgataaga
     2281 aagtaatggc acttgcttgt gtgcccataa tactcagcaa aaccaagaac caaggcatgg
     2341 attgattgct tcggaaatac ccgtcgagat cttttgtagt ccgactgcgg tacaagccat
     2401 acacaattat gacacttaag gtcagcacca atacaatcca gtcgatgcta ctcatgcaaa
     2461 agcggcggta aacatgtaaa acagtatgat taaaaggagc aaaacactta ttacgagtgc
     2521 atacattcct ttccaagagc ccatgatagg cgctttttcg ttaggatcag tttccgtcat
     2581 tttttcccca agctatcata tttgcaaaca aacgataagc acccggtaca ccagctggaa
     2641 gttctcggaa aaacgacaat cctgtataaa tgaattttcc ttttccatac tcagttacga
     2701 ttaaagcacc ttgcaatggt ttttcacccg gatcgttaca ttcgagcaaa gcagtgtaat
     2761 gtgaatccca tttttcagga aaatacaatc cacgttcttg aatccagcca gtaaaatcct
     2821 gatcggtaat ttggttgggg gtattaaaaa tcggatgatc tgtaatcaaa cgtttcattg
     2881 gagcattttc gtcggtcaca cgctcacgag aaacttcaaa aggaaaaggc cccggttgaa
     2941 cgcttacatc gcttatccaa ttcttagtat tgtattgttc aattaaggtt ccaccttgtt
     3001 gcacgtatgc taataaaatc ggcatcagac tggacatatt ttcattcgta ttaaatgccc
     3061 gaattccaat aattacggca tccacatttt tcaatttttc agtcgtcaca tctcctgaag
     3121 ctattgtatt tactgtgtaa cctgcttgtt ttagacaatc gcctacttta tcgtcggcac
     3181 cgggaatgta aacgatgttt tctcctacac gatttaggtt tacgtaaact gctttgaatg
     3241 ccgcaggctt tatccaagtt tgaattggaa tatgatcgta atcaatgcga ctaatatgca
     3301 tcaactcttt tttatttgtg ccttccatca tcatcacccc aattgtaaaa actgcatctt
     3361 gttttgtttc gcggctagga ttagcagata aatcgaattt gataatttgt ttacgaggag
     3421 ctccgtttcc caattgaacg gatattttgg aaggagaaga atgaaaataa gcgggtaaat
     3481 cgagagtgac atcgatgttt gctgaattag cagatgtttc aatggtgatt tctactgtac
     3541 gatggtcact ttgattgaga ataaaaacat cttcattaac agttgctgtt attgctggca
     3601 aaacttctac tcttctagtt aattcacctt ttacaggatc aacccagagg tagtttaatg
     3661 gaaatctttt aagaaaatgg attgttccaa tttgccattc tgtttcaatt tgaatgggcg
     3721 cttctgattc aggtttccca atttgactta attcgttcac ggtaaataaa ccttccgcat
     3781 ggggagaatt aagccaataa ggatttgata tggctaattg agcaggtagt tttaacgtat
     3841 cgtttcgagt gtagacttca ttattcgtca ggactttatt acattcaact aatatagaat
     3901 tcggtaaaat agctcgtgtt gaaagcagtt ttatttcctg attagatcga ttgatacatg
     3961 aaaaactcac aggaacagct tcacctggat taactgcaaa tttattcgcc ttgaattcac
     4021 cataaaatcc agcacattga agaataatgt cttgcaaaag ttgaacatga tgatcggaat
     4081 tctcaagtgg tgaaagtttc tgaaaagctt gataaagtcg cgttaatgcg ggaatactgg
     4141 atgctggatt ttcaattttg aattgttgaa tgatgctgtc gatcgaatct gaaatggctt
     4201 tgttcttcca accttgcttt aggttttcga atggttctgt ttgggctttt tcgcccgcta
     4261 gatgatcgaa atattctaaa atttcacccc gttgaagtgc aactccaaat ccctgacttt
     4321 tatgctgact gcggctttta gcagatattt caccaatcga ttctcccaat aaaggcaaat
     4381 aagaaccaat gtcgagttta agtgcggatg aaacatcggc tttgggatcg cgccatttgc
     4441 ggaaattatt ccaataaagc gatttcgttt tccatgttcc ataaagcttt gcagattcgg
     4501 gaaatcgttt ttcatccgca gcagcttcga aagcttcacc agcaaggatt gcagaagcag
     4561 tatgatgtcc gtgtcctcct tcaccagttg ttgggaaacg agctataatc acatccggtt
     4621 tgaatttccg aatgactaaa accacatcac ttaaaatttt ctctttatcc caaattcgaa
     4681 atgtttcttc cggatttttc gaaaatccaa aatcgaatgc acgagtaaaa aactgctctg
     4741 caccatccgt atttcgagct gcaatgagct cttgtgttcg aattaagcct aattcttcgg
     4801 attgttcatt accaatcaga ttttgtccgc catcacctcg tgttagtgaa agataagccg
     4861 tacgataatg acgttcattc gctaaccagg aaagcaaacg cgtattttca tcatcgggat
     4921 gggcagcgat atacaaaacc cttccttgag aattcaagtg atgaagttga gatattattt
     4981 gccctggagc tgattgtgat tgaatcgagc caaagaaaaa gcagaataaa ttaagcagta
     5041 ctagttgctt catgtatggg ttgggattta ctgaaattta agatttaaac agatgaatca
     5101 aaaaatcgtt caatacagcg atggaacctt ctaagtccca accttcgcga tttctaggtt
     5161 caactggatt ggaaatagaa cgtatttgca ctactggaat ttgctcctgt agtccaacat
     5221 aaaatacagc tgcaccttcc atactttcaa tttgcgcttc gggatttaat cgtttcattt
     5281 taagaattga ttgctcttcc ccatgaacgg tttgaacggt aattccttta actgcttgta
     5341 ttgatctaaa tttttcaatc agttttgaat aattttcagg aaatggaatt gattgtagaa
     5401 tgtcgaattg ttcgggctga agtccgattt gtgaagccgg tagaaaagta tcgtggtgtt
     5461 ctgcaccaaa agcgaaaaag tgatcactgg ttacgtttac taaagacttt cgaagaatta
     5521 aaggatcaat cgaaccagca attccgacat ttaataacaa atcaggacga aaataatgga
     5581 gcgatttcgt caattgatag gtagtcgcaa ccattccggc acccgtaatg agcactttta
     5641 gctgaaaatc gcctaggata ttcacttctt cctcaagaaa attcaaattc ttataaaagg
     5701 gagcaatttc ggcttgggta gctgcaatta gcagtatctt catgcttcaa atatacacgg
     5761 acagacgagt ttgtcttcta attttaaagg aaattctcca cagtagcgca ttctggaatg
     5821 ttaatcgaaa cagattgtta tttttgtccc acaaacctgc ccgatgttag aaacttcttt
     5881 gtcccgcatt caatggactt ttagagtatt attaatggcc atgctaggaa cttggttatt
     5941 atccaataaa gtatggatga cggagcggaa cttccctgta atacccatat ttgatggtat
     6001 tccatcgctt ccttcaccag tagattatat tttctatagt atacttatcg tattagcgat
     6061 ttatcagatt atttatttag agaacaggaa tacaaaaatt gccttggttg tattacttgg
     6121 atttctgatg attggtgatc aaaatcgctg gcaaccatac aatgttcaat atttcttaat
     6181 gatttcggcc ttgttttttt atcccaagtt taccgatggt attttgaatg tttttcgaat
     6241 gatggtgatt gtattcttca tttttagcgg aatacaagag ataaatactg tttttattga
     6301 gtcgattttc ccctggatga ttggtccaat tgcatcgtta tttcctgcta gtgtcgaacc
     6361 aaacatttta ggcggaggat atattttccc attcctaagt attgctgcag gtgtaggatt
     6421 attatttcaa caaactcgtg attacgctgt ctatttgggg attttaattc acctatttta
     6481 tttttatgca ttaagtccga tggggaacga ttggaatcat gccatcctcc catataactt
     6541 atgcatggca tttttcactt atgtgttgtt tttcagtgct gagttcaaat tgaaagatat
     6601 tttctggtca aaaacatttc gttatcaaca agctgtggta ataatttttg ctgttttgcc
     6661 tttgcttagt ttttttggga tatacgatcg gatgcagagt tttaatacat actcaggtaa
     6721 atctttgtat gctaaaattt atgtgacaga atccttagtt gaattactac ctgatggtgt
     6781 taaacgttat gtttatcgtc ctcaacaagg tgaaccttat attgaaacta cctattggtc
     6841 ggctgatgca ctcaaagttt ctccttatag cgaagaacga gtttacaaga aattacacga
     6901 atatgtttgt tcgttttccg aaggtgattg ttctgcccgt ttggaaatat actcatacgg
     6961 tggcggacac taactaggat tttctatagt ttaatttctg ctaattatgg ttttgaggat
     7021 tctttccctt tcattcttgt ttatcctcac tcaaactggg gtgaaatcgc aagagaatct
     7081 ttccaagcga attgaccaat atgagaatca attaaagtca cttcaagccg accaagagaa
     7141 agtacttcaa gagttggaag atttgaaact cgaaaatctt cgatcagatc ttcagaaaaa
     7201 tggcttacct tctttacttc caggtgaaga agtcattatg catgcagcca tggctttggt
     7261 ttacaacgaa gaatacgaac aagcaagttg ggtcgcacat atcattttac cagatgtagt
     7321 aagtggtatg gctggccgta gtaatgactt ccgccctgat cctctaatca aaactggttc
     7381 agctgttgag cgtgattact taatcaaaga cacgctggca aatggaacat acaagtatga
     7441 tggttttggt tttgatcgag gacatttagc tccttctgcc gatttccgtt ggtcgaaaaa
     7501 agcgttgtcg gaaagttttt attattcgaa tatgtcgccc caagtggctg attttaatcg
     7561 ggatagctgg gcgaaactgg aagatttgtt gcgtgcatat gtgcaagaga agaaagttcc
     7621 attgtatgta ctaactgctc cccttttacg tccaaactta cctaaaattg aacgcggagt
     7681 aaatcatgtt gccatacccg tttattacta taagattgct ttagatcctg tgaataaaca
     7741 agctattgcg ttcttaatgc caaataaaaa atgtgaatat ccaaccgagc attatgcagt
     7801 aagtattgat agtttggagc atttaactgg aagagatttt tattcttctt tacctgattc
     7861 attggaaaat gaattagaaa aaatggattc agcgaaacct tggatgaagt tgaaagagaa
     7921 gaatgatgaa aaacctttag atccaactca attacctaaa aagcacttca ataccgttca
     7981 ggcaaagttg tatcagaatc agaatgaaat cattaccgtt tgtggaactg tagtaagcac
     8041 caaattatct tcaaaaggga atgtgttttt gaatttagat aagggatttc ctaatcagat
     8101 atttacggta acgattttta aagatcagtt agtcaacttt acgtacgcac ctcaagtgga
     8161 attaatgggg aaagtaatat gtgtagaagg gaaagtggcc gatttcaatg gaacaccttc
     8221 tatggttatt gaacacgaaa aatcagttcg gctttattta gaagatgacg aacggtaaat
     8281 gttttcaatt ccctgataaa aatcattgcc agaagcgaat tgcagcttta cttttgaaag
     8341 aaaaaatcat gaatctgcat atcgaaaaac acatgactct tgcagaagtg caggcggctt
     8401 ttaatcacag ttttcctttt ttgaaaattg gtttctttat cgataaaaac cacgataata
     8461 ccctaactgc cgacgagcag atacacaatc ttaaaatgac
//
LOCUS       DAOOQW010000087         8442 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_10388_length_8442_cov_5.433170, whole genome shotgun sequence.
ACCESSION   DAOOQW010000087 DAOOQW010000000
VERSION     DAOOQW010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 8442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8442
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10388_length_8442_cov_5.433170"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            160..561
                     /locus_tag="PK281_06405"
     CDS             160..561
                     /locus_tag="PK281_06405"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HOY49013.1"
                     /translation="MERKEFLKTLALGTVAICAGACVSAKSTSSKPSTVEIDLSNPVN
                     EALKNKNGYVIQDGLIVINTGEDLPVVLSAACPHMGKKVYWDPSVSALVCPAHGSRFD
                     LAGKPTKGPAKEALNHYAVKKAGDKLTISLL"
     gene            678..2906
                     /gene="rnr"
                     /locus_tag="PK281_06410"
     CDS             678..2906
                     /gene="rnr"
                     /locus_tag="PK281_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007095872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HOY49014.1"
                     /translation="MAKKHKKKKDKVLVKLLLDDVLKYFQDAPTTMHNYKDVCAGIGA
                     TDPMDRIQVMEVLEALVANAQLSEPEPGKFRYSSMGSSFVDGTVQLTTGGAGYIISPE
                     SAEDVYVRENYLLNALNGDKVRVHLFARRKGKKLEGEVVEILERSKTQFVGIVQMSAK
                     FAFVVPDSNKMPIDIFVPLGELHGAKDGDKVLVKMMDWPEHAKNPFGSVVKVLGKPGD
                     NNVEMHAIMLDFELPYEFPQEVEDAANAIPNEIPEKEIKRRRDFRGITTFTIDPADAK
                     DFDDALSIQKLPNGNWEVGVHIADVSYFVKPGSILEEEAYKRATSVYLVDRVVPMLPE
                     HLSNGVCSLRPDEDKLTYSAVFELNDEAEVQNQWFGRTVIRSTRRFAYEEAQKVIETG
                     EGDLKEEILTLNRLAHIMRDRRYQRGSIAFDKVEVKFRLDKDGKPLGVYFKVQQEANE
                     LIEDFMLLANRSVAEFISQMGKKEGDKRNAARKPFVYRVHDKPDPEKLMEFSRFVAQF
                     GYKFKFQSEAQISHQMNDLMKEVKGKGEANVIETLAIRTMAKAIYTTKNIGHYGLGFK
                     FYTHFTSPIRRYPDVMVHRLLDAYMDNRNWEAETELETRCKHSSEQEKRASDAERASV
                     KYKQVEFLKDKVGEEFRGVISGVTEWGIFVEILENKCEGLVRLRDIPDDYYFFDEESY
                     SIIGKDHKKRYRLGDEVTITVKRADLVKKQLDFSLLDERPRRISSPAFVKEPKKRNRY
                     RR"
     gene            2954..4789
                     /gene="uvrC"
                     /locus_tag="PK281_06415"
     CDS             2954..4789
                     /gene="uvrC"
                     /locus_tag="PK281_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009641087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HOY49015.1"
                     /translation="MDASAYVLSILKTLPEKPGVYQYYDSEGKILYVGKAKSLKKRVY
                     SYFSKDHHDNGKTRVLVSKIADIKTIVVDSELDALLLENNLIKKYQPRYNVALKDDKT
                     YPWICIKKERFPRVFPTRRMVKDGSEYFGPYPSVKVMRTLLDFIKALYTRRTCNLALE
                     PVAIKNGKFKVCLEYHIKNCLGPCVGYQSEQDYMQTIQEIRQLVKGNLGELIQLIRGR
                     MLEHASKFEFEQAHELKERLELLEKFKSKSTIVNANIHNVDVFSIHSDEKIAYVNYLK
                     VLEGSIVQGHTIELKKRLDESPEELLSIAIGEMRQRFNSTAKEIIVPIMPDLEDDFLT
                     ITLPQRGDKKQLLDLSTRNVLNYLREKNKQIELVDPERHTKRILEKLQKDLRMPVLPK
                     HIECFDNSNFQGTYPVSAMVCFKDARPSKKDYRHFNVKTVVGPDDFATMAEVIERRYS
                     RLLEENEPLPQLIIVDGGKGQLGAALGALDKIGLRGKISIVGIAKRLEEIYFPGDPLP
                     LYIDKKSESLKLIQHLRNEAHRFGITHHRKRRDNGTLKTELSEIEGIGKSSAEKLLLD
                     FRSVSKIRELTIEQMEQSVGRARAEKVFAFFHPEIANPENSQKDS"
     gene            4812..5777
                     /locus_tag="PK281_06420"
     CDS             4812..5777
                     /locus_tag="PK281_06420"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOY49016.1"
                     /translation="MKIIVTGAAGFIGSHLCGSLIEQGHRVIGIDNLSRGEEVSIHAL
                     EHHPNFEFHVEDAGNWELLNSLQADLVIHLASQKIPRYSSGWETLQENARVSNTVLDY
                     CIKKNVRLLFASTSDVYGKNPTIPYVESSDCLIGPSHIKRWAYATSKLHTEQLMFAAG
                     REFGLRFQIMRFFGCYGPRQAKGWWGGPHSVFMERALMGEELEIHGDGKQTRCFIYID
                     DLIRGILTLVDRPELENGIFNFCSAEEEEIAIVDLAKMVWNSIRKDEAQIRFIPYAQF
                     GDYEDVMRRVGSSAKAEKLLNWKANISLEEGLKRTIDWQLGKGNV"
     gene            5774..6490
                     /locus_tag="PK281_06425"
     CDS             5774..6490
                     /locus_tag="PK281_06425"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOY49017.1"
                     /translation="MNLAIVVPVFNELENIPGLIETIETHCQDKTALTWVFCDDGSTD
                     GTYEWLFNESKKGTLHLTGDGKNHGPGKAFENGFQYVLNKLPECEAVLTLEGDGTADI
                     TSLDSMLKQLQNHDCVLASVYYPGGGFSKTNPFRLFVSKVANGLTRMLLRLPFRTLTS
                     FYRLYNRKALESLSKNGNLVLEETGFICQVEILLKLHRAGLSIVEVPTVLFTDRRKGQ
                     SKMNMGKTLISHLRFILKHS"
     gene            6518..7159
                     /locus_tag="PK281_06430"
     CDS             6518..7159
                     /locus_tag="PK281_06430"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002679146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HOY49018.1"
                     /translation="MEFIDEKLAQYANDHSSKESELLYRLNRETHLKVMQPRMLSGHL
                     QGRFLSLISHLVKPELILEIGTYTGYSALCLAEGLAENGKLITIDNNPERDELVKRYI
                     AESDLTGKVIPMIGDAHDLIPHLEGTFDLVFIDADKSSYADYFDLVIDRMRKGGIILS
                     DNVLWSGKVTEPVKSNDQDTRLLLEYNRKLANDPRIETVLLPIRDGISVARRK"
     gene            complement(7195..>8442)
                     /locus_tag="PK281_06435"
     CDS             complement(7195..>8442)
                     /locus_tag="PK281_06435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOY49019.1"
                     /translation="GILAGDKIVKIEGENMAGIKIKNKDVISRLRGTRGTIVKVSVFR
                     KGVKNLIDYSITRDKIPLTSIDATYMADSETGYVKLSRFSATSGTEFTESLEKLKGKG
                     MKNLILDLTGNGGGYLQTAIDIADQFLTNENLVVYTQGRTVASKAPYYSTSKGMFEKG
                     KLIVLIDEGSASASEIVSGAVQDLDRGLIIGRRSFGKGLVQNSFPFPDGSAMRMTTAR
                     YYTPSGRCIQKPYEKGVDDYYDDLNKRFKHGEFTNRDSITFADSLKFYTIGKRLVYGG
                     GGIMPDLFIPLDTSENSKYLTDVYRKGIFNKFTLKYVDQNREQIKKQYPDFKAFKAGF
                     TTSDAIMNEFIAAAEADEIKSNPDDIKTSGSFIRRQIRALIANQIWGTNEYYECINEG
                     NPILKEALVQMKSENFKKLKLRY"
BASE COUNT         2608 a         1434 c         1945 g         2455 t
ORIGIN      
        1 tcccaaagcg ccaaatcgtg tagcttccca taacgcagta gtcgggattc aggtaggctt
       61 ttactactta tttaacggtc ctccaatggt tcataaacca aagctacgtt agtattttaa
      121 ttttagtctg tatattccga tttcaataac aaggtatata tggaaagaaa agaatttctt
      181 aaaacacttg ctcttggcac tgtagctata tgtgccggag catgtgttag tgcaaagtcg
      241 acatctagta aaccttcaac agtggagata gatttatcta atccagttaa tgaagcactg
      301 aagaataaaa acggttatgt aattcaggat ggcttaattg tcatcaatac gggtgaagac
      361 ttaccggttg tattatcggc agcttgccca catatgggta aaaaagtcta ttgggatcct
      421 tcagtttcgg cacttgtttg tccggcacat ggctcacgtt tcgatcttgc tggtaaaccg
      481 acaaaaggtc cagctaaaga ggcattgaac cattacgctg ttaagaaagc tggagacaag
      541 ctaacaatca gcttattata aaatttcaaa tcaatgcatg gtgaattgct tgattagttg
      601 aaaaacggtg tattcttctt ttcgggttag attaactcct cgcctgcaat gtgtactttt
      661 accgaaattc ccaccccatg gcgaaaaaac acaaaaagaa gaaagataag gtcttagtta
      721 aacttttgtt ggatgatgtt ctgaagtatt ttcaagatgc tccaacaact atgcataact
      781 acaaagacgt atgtgctgga attggtgcaa ctgatccaat ggatcgaatt caagtgatgg
      841 aagtgttaga agcgttggtt gctaatgctc agttatctga acctgaacca gggaaattcc
      901 gctatagcag catgggaagc agctttgtag atgggacagt tcaattaaca acgggtggag
      961 caggctatat aattagtcct gaaagtgccg aagatgttta tgttcgcgaa aattacttgc
     1021 tgaatgcttt aaacggggat aaagtacggg ttcatttatt tgctcgtaga aaagggaaaa
     1081 aactcgaagg agaagtcgtt gaaatattag aacgttcgaa aacgcaattc gttggtattg
     1141 ttcagatgag tgccaagttt gcattcgttg ttcctgatag taacaaaatg cccatcgaca
     1201 tatttgtgcc ccttggagaa ttacatggag ctaaagatgg cgataaggtt ttagtgaaaa
     1261 tgatggattg gcccgaacat gctaaaaatc catttggttc ggtcgtcaaa gttttaggta
     1321 aacctgggga taataatgtc gaaatgcatg caatcatgct tgatttcgag ttgccgtatg
     1381 aatttcctca ggaagtggaa gatgcagcga atgcaattcc aaacgaaatt ccagagaaag
     1441 agataaaaag acgtcgggat tttagaggaa ttaccacgtt tacgattgat ccggccgatg
     1501 caaaagattt tgacgatgcc ttatctatac agaaattacc gaatggaaat tgggaagttg
     1561 gagttcatat tgccgacgtt tcctactttg taaaaccagg ttccatctta gaagaagaag
     1621 cctacaaacg agctacttct gtttatttgg tagatcgtgt tgtgcccatg ttaccggagc
     1681 atttatcgaa tggcgtttgt tctttgagac ccgatgaaga taaactcacc tattcggcag
     1741 tatttgaatt gaatgacgaa gctgaagttc aaaatcaatg gtttggtaga actgttattc
     1801 gttctaccag acgatttgca tacgaagaag ctcaaaaagt aattgaaacg ggtgaagggg
     1861 atttaaaaga agaaatcctg acacttaatc gtttagctca tatcatgcga gatcgacgtt
     1921 atcagcgagg ttccattgca tttgataaag ttgaagttaa gttccgctta gataaagatg
     1981 gtaagccact tggagtttac ttcaaggttc aacaagaagc taatgaactg attgaggatt
     2041 tcatgctttt agctaaccga agtgtggctg aattcatttc tcaaatgggc aaaaaagaag
     2101 gcgacaagcg aaatgctgcg agaaagcctt ttgtatatcg cgttcacgac aagccagatc
     2161 ctgagaaact catggagttt tcgcgatttg tagctcagtt tggatacaag tttaaatttc
     2221 aatcggaggc tcagattagt catcaaatga acgatttgat gaaagaggtg aaaggaaaag
     2281 gagaagcaaa tgtgattgaa acattggcta tccgaactat ggctaaggct atttatacaa
     2341 ccaagaatat tggtcactat ggattgggct ttaaatttta tacgcacttc acttctccta
     2401 ttcgtagata tccagatgtg atggtacatc gacttttgga tgcgtatatg gataaccgaa
     2461 attgggaagc tgaaactgaa ttagaaacgc gttgcaaaca ctcatcagaa caagagaaac
     2521 gtgcatcgga cgccgaacgt gcttcagtta agtacaaaca agttgaattc ttgaaggata
     2581 aagtaggtga agaattcaga ggcgtgattt cgggtgttac cgaatgggga atttttgtgg
     2641 agatactgga aaacaaatgt gagggattgg ttcgattgag agatatacca gatgattact
     2701 acttctttga tgaagagagt tattccatca ttgggaaaga ccataaaaaa cgatatcgct
     2761 tgggtgatga agtaaccatt accgttaagc gtgccgattt ggttaaaaag caactcgatt
     2821 tttctttgct tgatgagcgc ccaagacgta tatcttcacc agcatttgtg aaagagccta
     2881 aaaaacgtaa ccgatatcga cgataagatt ctacctctca ttcttattga tactgttgga
     2941 atttagctga aagatggatg ctagtgcata tgttctaagt atactcaaga cgcttcctga
     3001 aaagccgggc gtatatcagt attacgattc ggaaggtaaa atactatacg ttgggaaggc
     3061 taagagtctg aaaaaacgtg tgtattcata tttcagtaag gatcatcacg acaatggaaa
     3121 aacgcgggtt ttagtatcga aaatagccga tatcaagacg attgttgttg attcagaatt
     3181 ggatgctctt ttacttgaga acaacttaat taagaagtat cagccacgct ataatgtggc
     3241 acttaaggac gataaaacgt atccttggat ttgtatcaaa aaagaacgat ttccaagggt
     3301 gtttccaacg cgtagaatgg taaaagatgg ctccgagtat ttcggtccct acccatctgt
     3361 taaggtaatg cgcaccttac tcgatttcat taaagcactt tatactcgac gaacttgcaa
     3421 tcttgccctt gaacctgttg caattaaaaa tgggaaattc aaggtgtgtt tagaatatca
     3481 tattaaaaac tgccttggac catgtgtagg atatcaatcg gagcaggatt acatgcaaac
     3541 gattcaggaa atccggcaat tagttaaggg aaatctaggc gagctgatac agcttatccg
     3601 aggaagaatg ctcgaacatg caagtaagtt tgaatttgag caagcgcatg agttgaaaga
     3661 gcgattagaa ttgctggaga aattcaaaag caaatcgacc attgtgaatg cgaacattca
     3721 taatgtagat gtattctcga ttcattcgga tgagaaaatc gcatatgtaa attacctaaa
     3781 agtactagaa ggaagcattg ttcaaggaca taccatcgag cttaagaaac gcttggatga
     3841 aagtcccgaa gagctcttga gtattgctat tggagaaatg cggcaacgct ttaatagtac
     3901 tgccaaagag attatcgtcc caataatgcc cgatttggaa gatgatttct tgacgattac
     3961 actacctcaa cggggagata aaaagcaatt gctcgatttg agcactcgga atgtgttgaa
     4021 ttaccttcgg gagaagaaca agcaaattga gttagtcgat ccagaacgtc atacgaaacg
     4081 aatcttggaa aaactgcaaa aagatttacg catgccggta ttacctaagc atattgagtg
     4141 cttcgataat tcaaattttc aaggtacgta tccggtatcg gcgatggttt gttttaaaga
     4201 tgcacgaccg agtaaaaaag attatcgtca ctttaatgtg aagacagttg ttggaccgga
     4261 cgattttgct accatggcag aagttattga acgtcgctac agcaggcttt tggaagaaaa
     4321 tgagccatta cctcaactga taattgtaga tggtggaaaa gggcaattgg gagccgcttt
     4381 aggtgcttta gataaaattg gattgcgagg gaaaatcagc attgttggta ttgcaaaacg
     4441 cttggaagag atttattttc caggtgatcc gctaccctta tacatcgata agaaaagtga
     4501 gagtttgaaa ttgattcaac acttacggaa tgaagcgcat cgttttggga ttacgcatca
     4561 ccggaagcgc cgtgataatg gcacattgaa aactgaactt tcggaaatag aaggcattgg
     4621 aaaaagttcg gctgaaaaac tgttgttaga ctttcgttcc gtttctaaaa taagagaatt
     4681 aactatagaa cagatggaac aatcggttgg aagggcgcgt gccgaaaagg tatttgcgtt
     4741 ttttcatcca gaaattgcta atccggaaaa cagtcaaaag gatagttaag ttttagcacc
     4801 tttgtggttc catgaaaata atcgttactg gcgcagcagg atttattggc tcgcatttat
     4861 gcggaagtct tattgagcaa ggccatcggg ttattgggat tgataatttg agccgtggtg
     4921 aggaagtttc tattcatgcg ctggaacatc atcctaattt tgaatttcat gttgaagatg
     4981 ctgggaattg ggaattacta aattctctcc aggctgattt agtgattcac ctcgcttctc
     5041 aaaaaatccc caggtattca tcaggatggg aaacacttca agagaacgct cgagtttcta
     5101 acactgtatt ggattactgt ataaaaaaga acgttcgttt actatttgca tccacaagtg
     5161 atgtatatgg caagaatccc acgattccct atgtagagag cagtgattgc ttgattggtc
     5221 cttcacatat taaacgatgg gcgtatgcta cttcgaagct acatacggaa cagttgatgt
     5281 ttgctgcagg tcgtgagttc ggattgcgtt ttcaaatcat gcgattcttt ggatgttatg
     5341 gtcctagaca agcaaaaggt tggtggggcg gaccacattc ggtttttatg gaaagggcgc
     5401 tgatgggaga agaattagaa atacatggag atggaaaaca aacgcgctgc tttatttaca
     5461 tcgatgattt aatccgtgga attctcacac tggttgatag acctgaatta gaaaatggca
     5521 tttttaattt ctgctcagca gaagaagaag agatagctat cgtagatctc gctaagatgg
     5581 tttggaacag catacgaaaa gatgaagctc aaatcagatt cattccttat gcacagtttg
     5641 gagattatga agatgtgatg cgtagagttg gttcctcggc aaaagcggag aaattattga
     5701 attggaaagc caacatttca ctggaagaag gactcaaacg taccattgac tggcaattgg
     5761 gaaagggcaa cgtatgaatt tagctatagt cgttccggta tttaatgagc tggaaaatat
     5821 tcccggattg attgaaacaa ttgaaacaca ttgccaagat aaaacagcat tgacttgggt
     5881 tttttgtgat gatggatcaa ctgatgggac ttacgaatgg ctgtttaatg agtcaaagaa
     5941 aggcacctta catttaactg gtgatggaaa aaatcatggg cctggaaaag cctttgaaaa
     6001 tggatttcaa tatgtgttaa acaagctccc tgaatgtgag gcagttttaa cacttgaagg
     6061 agacggcaca gctgatatta cgtcattaga ttccatgctg aagcaattgc aaaatcacga
     6121 ttgtgtgttg gcttcggttt actatccagg tggaggattt agtaagacta atccatttcg
     6181 cttatttgta tcgaaagttg caaacggatt aactcgaatg ttacttcgat tgccctttag
     6241 aactcttacc agtttttatc gtttatacaa tcgaaaagca ctggaatcat taagcaagaa
     6301 tgggaattta gtgttggaag aaactggatt tatttgtcag gttgaaattc tgttgaagtt
     6361 gcatcgggca ggattaagca ttgtggaagt accaacagta ttgtttaccg accgaagaaa
     6421 agggcaatcg aaaatgaaca tgggaaagac cttgatttca catcttcgtt ttatcctaaa
     6481 acatagttga aagcactcaa aaatatcgaa cacctgtatg gaattcatag atgagaaact
     6541 ggctcaatat gccaatgacc atagtagtaa agaatcggaa ttgctttatc gattgaatcg
     6601 ggaaacgcat ttaaaagtga tgcaaccgag aatgttatcg ggacatctcc aaggtcgttt
     6661 tttaagtttg attagtcatt tagtgaaacc tgaattaatt cttgagatag gcacttacac
     6721 gggctattca gctttatgtt tagcggaagg attagccgaa aacgggaaat taattacgat
     6781 tgataataat ccagagcgtg acgaattggt gaagcgctac attgctgaat ccgacttaac
     6841 cggaaaagta attcccatga ttggcgatgc gcatgatttg attcctcatt tagaaggaac
     6901 atttgactta gtgtttattg atgcagataa atcgagttac gcggattact tcgatttagt
     6961 gattgaccga atgcgaaaag gtggaattat actttcagac aatgtacttt ggagcggaaa
     7021 ggtgactgaa ccagttaaat cgaatgatca ggacacacgt ttattattgg aatacaatcg
     7081 caaactagcc aatgacccaa gaattgagac cgtattactt ccgattcgag atggaataag
     7141 tgtagcgaga aggaaataaa aaagggcgct gttggcgccc ttttttatgg ttgattagta
     7201 ccgaagttta agctttttaa agttctctga tttcatttga accagcgctt cttttaagat
     7261 tgggttgcct tcgtttatgc attcgtaata ttcatttgtt ccccaaattt gattggcaat
     7321 aagtgcacga atttggcggc ggatgaagct gccagaagtt ttgatatcgt cagggttgga
     7381 tttgatttca tcggcttcgg cggcagcgat aaattcgttc atgattgcat cactagttgt
     7441 aaaaccggct ttgaaggctt taaaatcggg atactgtttt ttgatttgtt cccggttttg
     7501 atctacatat ttcaaggtga atttgttgaa gatacctttg cgatatacat cggttaggta
     7561 ttttgaattt tcggatgtat cgagtggaat gaacaaatca ggcatgatgc cacctccacc
     7621 gtatacgaga cgtttaccaa tggtgtagaa tttaagcgag tcggcaaatg tgatgctatc
     7681 gcggttggtg aattcgccgt gtttgaaacg tttgtttaga tcgtcgtaat aatcatctac
     7741 acctttttcg taaggttttt ggatacatct tccagaaggt gtgtagtaac gagcggtagt
     7801 catacgcata gctgaaccat cggggaaagg aaaggaattt tgaacgagac ctttaccaaa
     7861 agagcggcga ccgatgatga gtccgcgatc gagatcttga actgcacctg agacaatttc
     7921 ggatgcagaa gctgagcctt catcgataag cacgatgagt ttacctttct cgaacattcc
     7981 tttagaagtg gaataatacg gcgcttttga agcaacggtt ctaccttgtg tgtaaacaac
     8041 aagattttcg ttggttagga attgatcggc aatatcgata gcagtttgca agtaaccacc
     8101 accgtttcct gtaaggtcga ggataaggtt cttcattcct ttgcctttga gtttttcgag
     8161 gctttcggtg aattcggtac ctgaagtggc gctaaaacgg ctaagtttca cgtaacctgt
     8221 ttcagaatcg gccatgtagg tagcatcgat actggtgagt ggaattttat cgcgggtgat
     8281 gctgtaatcg attaggtttt taacgccttt acgaaatacg ctaactttaa caatggtacc
     8341 gcgcgttcca cggaggcggc taatgacatc tttgtttttg attttgatgc cagccatatt
     8401 ttcaccttcg attttcacga ttttatcgcc agcaagaatt cc
//
LOCUS       DAOOQW010000088         8348 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_10541_length_8348_cov_4.946220, whole genome shotgun sequence.
ACCESSION   DAOOQW010000088 DAOOQW010000000
VERSION     DAOOQW010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 8348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8348
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10541_length_8348_cov_4.946220"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(23..493)
                     /locus_tag="PK281_06440"
     CDS             complement(23..493)
                     /locus_tag="PK281_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008990374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOY49020.1"
                     /translation="MQRHHHYNSTIKWTGNNGTGTDNYRNYERSHQIIIENKSIILGS
                     SDPAFRGDKTKHNPEDLLVASISSCHMLWYLHLCAEAGIVVLDYIDNAIGIMIESSNG
                     GGQFTEVILNPTVVVTENSMSDRAKELHRKANELCFIANSVNFPIHHNPIIKIA"
     gene            complement(607..1890)
                     /locus_tag="PK281_06445"
     CDS             complement(607..1890)
                     /locus_tag="PK281_06445"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HOY49021.1"
                     /translation="MKNLISFLMCLFVNLSIHAQGNYIHTSGRHILGPCGDTLLIQGV
                     NYAPYNWGYLADELRLDQIAQSGANAVRLVWYTSNNAAPVYANLDALDSAITRCTQLG
                     MIAIVELHDFTCSNDAAGLITGLSWWEQPEVINLLDRNKESIIVNISNESLYYNWGSN
                     PAQSLSTYFSTYSTIISNLRAHPEFKFPLLIDAPDCGGSSDAFVTASTAANLIATDPE
                     QNLIFSTHTYWYAQTNNDSSLIYQRLANVCNANIPLIAGEVANSQDDTELCQYALNYS
                     AVINSCNSLQMGWLIWCWDRDGCPDRQMSSDGNFAHLTTLGNDILNNSNYGWTVHPPQ
                     KSKYLTTADCSLGNNAINDTRFNIQISKSNNQLNLSSTSTIQQIRLFDAQGKVYPILP
                     NGTNTYLSNELIPGIYFIQAQDSKGNSFHQKFLVN"
     gene            2058..2351
                     /locus_tag="PK281_06450"
     CDS             2058..2351
                     /locus_tag="PK281_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008614702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HOY49022.1"
                     /translation="MRIISRRTLRDFWEKHADSKEQLLSWFQEANSAQWTNPSDVKRE
                     YPNASFLKDNRIVFNIKGNTYRLIVRVNFDYQMIWIRFIGTHAEYDKIDANKI"
     gene            2356..2715
                     /locus_tag="PK281_06455"
     CDS             2356..2715
                     /locus_tag="PK281_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOY49023.1"
                     /translation="MTIKPIKNEQDYQATLSRLEEIFDAKKGTPEGDELEILSILIDN
                     YETHHFPIDFPDPIEAIKFRMEQMGYNQSDLAKVVGLKSRASEILSKKRKLTLEMIRQ
                     INVSLKIPTEVLIQAYK"
     gene            2764..3333
                     /locus_tag="PK281_06460"
     CDS             2764..3333
                     /locus_tag="PK281_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOY49024.1"
                     /translation="MKSIIKAIQSLQLLPEAELLVLNSLVKPRKLAKNDFFIREGNVC
                     REMVFIDSGILRSFYHTAEGTEVTSCIRFEQELMSAFSSFITQTPSEENIQALVETEL
                     WVLTYDELQSLYSRSVEWQQIGRILTEMQYVDSEKRIASFLKQSAQERYESLICEHAL
                     YVKYIPQHYLASYLGITPRHLSRLRKAMI"
     gene            3413..3991
                     /locus_tag="PK281_06465"
     CDS             3413..3991
                     /locus_tag="PK281_06465"
                     /EC_number="1.-.-.-"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006260988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOY49025.1"
                     /translation="MKKILVIQAHPDPESFNAALAKAYIQGAENAGAEVKLLVISELK
                     FDPVLRYGYRKRTELEPDLVLAWEWICWADHLVWVHPVWWGGLPALSKGFIDLLFLPG
                     MAFSYKENSNFQIKLLRGKTANILTTLDQPGWYYWLMYGRPSVNMLKRSTLQFCGINP
                     VQVNYFGIVRNSNAAQRKKWLRQARLLGEKLK"
     gene            4071..4484
                     /locus_tag="PK281_06470"
     CDS             4071..4484
                     /locus_tag="PK281_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49026.1"
                     /translation="MSQSIKFRIFVLSLGVFLCQFTHANAATKIDSGRKDTLEFTAED
                     AIRYAKFINFLRFREDWTAARTPNGDAWVVTAERDYLSKKIIRSTGSENQYFDKKKGA
                     LVRRQRMMTIHARSGKILGKQTKVEIRRNQAEGTK"
     gene            4771..>6797
                     /locus_tag="PK281_06475"
     CDS             4771..>6797
                     /locus_tag="PK281_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015362357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HOY49027.1"
                     /translation="MNSDAARVNIYHFQIFKSPHLQIIHYLRRMNINPHDDKRLFLLD
                     AFALIYRAYFAFSRNPLMNSKGMNVSAASGFTNTLVELMLKEKPTHIAVVFDSAAETN
                     RAVEHSFYKAHREAMPEDIQSNLPWIKEIISAFNIPMLELAGYEADDIIGTIAKVKAR
                     EGHVVYMVTPDKDYGQLVEPNIYMYKPARMGNDIEILGVPEICAKWEIDDPLKVIDIL
                     GMWGDASDNIPGIPGIGEKTAKKLIQQYGSMENMIAHSHELKGKMKENVEAFVDQAHI
                     SKMLATIILDVPIEVSDEDLLISEPDKEKLAALFTELEFRSMGRRILGADFSVNQQIA
                     PEVKKSIPAAPKPSNGQISMFGEENNPEEDHPEPAEKGKNISNTPHTYFRVESEEELK
                     NLADLLGQQAEFCFDTETSSLDYFSLELVGLSFSVKEGEAYYVPTPSDKEEVSRILSY
                     FKPLLESKTILKIGQNVKFDLLVLRQFGVEVSLPVYDTMLAHYLVEPDMKHGMDYLSE
                     TYLGYTPVSITELIGKRGKNQGTMRDVPVEQVVEYASEDADITLQLKHVIQPMVEKRE
                     VVDVLQKIELPLIPVLADMEYEGVKVDEAFLANYSEELQSDLILLRDAIWEMAGQEFN
                     IDSPKQLGEILFDKLKLPGAEKTKTGQFATGEEILSKLEKEHPIAAKLLDYR"
     assembly_gap    6798..6897
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6898..7572
                     /locus_tag="PK281_06480"
     CDS             <6898..7572
                     /locus_tag="PK281_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HOY49028.1"
                     /translation="DYSQIELRLVAEIAKEEAMLEAFTKGIDIHQATAARVYGVALED
                     VTREMRSKAKMVNFGIIYSISAFGLSQRLGVPRKEAAELIENYFAQYPGIKGYMSSTL
                     EFARANGYVKTMMGRRRYLKDINSRNFTIRGFAEREAINSPVQGSAADLIKMAMIGIH
                     QELKEKNLRSKMTLQVHDELVFDAHKDELEILKPIILHHMRTAFQTTVPIEVEIGTGM
                     NWLEAH"
     gene            7856..>8348
                     /locus_tag="PK281_06485"
     CDS             7856..>8348
                     /locus_tag="PK281_06485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49029.1"
                     /translation="MMKYFYACLFLLVSQLSLLAQQNTDNTQPPRCFTDEYLRAKREA
                     DPTFKTREAIQNILIQQQARPANDRSVLTIPVVIHVVYLPGQDPYGHYIDIYNGIDHL
                     NAAFSNSGQYHNALGVNTNIQFCLATTGPLGESTYGNPITYTESELSNLTIETQDAEL
                     KNLI"
BASE COUNT         2569 a         1474 c         1728 g         2477 t
ORIGIN      
        1 aattctctgc ataatgttag actcaagcta ttttaataat aggattatgg tgaattggga
       61 aattaacaga atttgctata aaacataatt cgtttgcttt ccgatgtaat tcttttgctc
      121 tatcagacat agaattttct gttacaacta cagtaggatt aagaatcact tcggtaaatt
      181 gtcctccacc atttgatgat tcaatcataa tcccaattgc gttgtcaata tagtctagaa
      241 caactatacc cgcttcagca cacaaatgta aataccaaag catatgacaa gacgatattg
      301 aagcgactaa caaatcttct ggattgtgtt ttgttttgtc tcctcggaat gcaggatcag
      361 acgaacctaa aataatcgat ttattttcaa ttattatctg atggcttctt tcataatttc
      421 tataattgtc agttccagtc ccattattcc cggtccactt aattgtacta ttatagtgat
      481 ggtgtctctg cataatcttt gaaatttgtt ttcaattagt ttgaatgctt tgtttttaaa
      541 ttaaatgctg tctttgtgga cctaaatgct tgccactatt tgttgatggg cggataaaat
      601 atcatattaa ttcacaagaa atttctggtg aaagctatta ccttttgaat cctgtgcttg
      661 gatgaaataa ataccgggaa ttaattcatt cgataaataa gtattcgttc catttggaag
      721 aattgggtac acttttccct gtgcatcaaa taagcggatc tgctgtattg ttgaggtaga
      781 ggagaggttc aattgattat tggatttgct gatttgaata ttgaaacggg tgtcgtttat
      841 tgcattattt cctaagctac aatcggcagt agtgaggtat ttactttttt gaggtggatg
      901 aacagtccaa ccataattcg aattattcaa aatatcattt cccaaagtcg ttaaatgtgc
      961 gaaattaccg tcgctgctca tttgtcgatc gggacaacca tcgcgatccc aacaccaaat
     1021 taaccagccc atttgaaggg aattgcaact attaattacg gcagaataat tcagtgcata
     1081 ttgacacaat tcggtatcat cttgtgaatt ggcaacttcg ccagcaatca gtggaatatt
     1141 ggcattgcaa acattcgcta aacgttgata aataaggcta gaatcgttgt ttgtttgggc
     1201 ataccaatac gtatgcgtgc tgaaaattag attttgttcc ggatcggttg caatcaaatt
     1261 ggcagcagta gaagcagtga caaatgcatc ggaagaacca ccgcaatcgg gtgcatcaat
     1321 cagtaatggg aatttaaatt cgggatgtgc tcgtaggttg gaaataatgg tcgaataggt
     1381 gctgaaatac gtgcttaatg attgagccgg attgcttccc caattataat acaagctttc
     1441 gttggagata tttacaataa tgctctcttt atttcgatcg agtagattaa ttacttcggg
     1501 ttgttcccac caagataaac ctgtgattaa tccagcggca tcgtttgaac aggtgaaatc
     1561 gtgaagttcg actatggcaa tcattccaag ttgcgtacaa cgagtaatag ctgaatctaa
     1621 tgcatctaaa ttcgcataaa cgggagctgc attattgctc gtgtaccaca ccaaacggac
     1681 cgcattggcg ccgctttgtg caatctgatc taatcttaat tcatcagcta aataacccca
     1741 attgtaggga gcataattca ctccttgaat cagaagtgta tcgccgcatg gaccaagaat
     1801 atgtcggcct gatgtgtgaa tataattgcc ttgtgcgtgt atggataagt taacaaaaag
     1861 gcacattaga aaactaatta gatttttcat aagacgaatt atttattcaa ttactgtttt
     1921 cgtattcggt atttaagaaa caaatctcaa atgtttcaaa actactgaat ggaatgtcaa
     1981 ataaagaaca ttaattactc actactttag cttcccaatt tgggaacttt gtttatcttt
     2041 gtaaaaacta atcgacgttg aggataattt cgaggcgaac attgcgagat ttctgggaaa
     2101 agcatgctga tagtaaagag cagcttcttt cttggtttca agaggcaaat agtgcacaat
     2161 ggacaaatcc ttctgatgtg aaaagggaat atcctaatgc aagcttttta aaagacaatc
     2221 ggattgtttt caacattaaa ggaaatacct atcgtttgat tgttcgggtt aatttcgatt
     2281 atcaaatgat ttggattcga tttattggca cgcacgctga atacgataaa attgacgcaa
     2341 ataaaatcta aagaaatgac catcaaacca attaagaacg agcaagatta tcaagctaca
     2401 ttaagtaggt tggaggagat ttttgatgca aaaaaaggaa ctccagaagg agatgaatta
     2461 gaaatattaa gcattctgat tgataattat gagactcatc attttccaat tgattttccc
     2521 gatcctattg aagcaataaa atttagaatg gaacaaatgg gttataatca gtcagattta
     2581 gctaaagttg ttggactgaa aagtagagcc agcgagattt taagcaagaa acgaaaactt
     2641 actttagaga tgattcgtca gataaatgtg agtctaaaaa tccctactga agttcttatt
     2701 caagcatata aatagtattc atttggatta atggaaattg aattaattaa attctgctaa
     2761 agtttgaaat caattataaa agcaatacaa tccctgcaac tattaccaga agccgaattg
     2821 cttgtgttga atagtttagt taaaccgaga aaattagcta aaaatgattt ctttattcga
     2881 gaaggaaatg tgtgtaggga aatggtattt attgatagtg gaatattacg ttcgttttat
     2941 cataccgctg aaggtacaga agtgacaagc tgtattcgtt ttgagcagga attaatgtcg
     3001 gcattttcga gttttattac ccaaacacca tcggaggaaa atattcaggc attggtggag
     3061 acggaacttt gggttttaac ttacgatgag cttcaatctc tgtattcacg aagtgttgaa
     3121 tggcagcaaa taggccgaat tctcactgaa atgcaatatg tggatagcga aaaacgaatt
     3181 gcttcttttc tcaaacaatc ggctcaagaa cgttacgaaa gtctcatttg tgaacacgct
     3241 ttatatgtga agtatattcc tcaacattat ctcgcatcgt atttaggaat tacaccaagg
     3301 catttaagtc gactaagaaa agctatgatt taaggacaaa tgtctagttc ggctatgctg
     3361 cgcccaattt agttttgttg cggaaggatt gaaaatttaa ccttcccgat acatgaagaa
     3421 aatactggta atacaggcgc atcccgaccc ggaaagtttc aatgcagctc ttgctaaagc
     3481 ttatattcaa ggtgctgaaa atgcaggggc agaagtaaaa ctgttagtta tttcggaatt
     3541 gaaatttgat cctgttttac gttatggata tcgaaaacgt accgaattag aacccgattt
     3601 agtactagca tgggagtgga tttgttgggc cgatcattta gtttgggtac atccagtttg
     3661 gtggggtgga ttaccagctt tgagtaaggg atttatcgac ctattgtttt tacccggaat
     3721 ggcattttcg tataaagaga attccaattt ccaaataaaa ttattgcgtg gaaaaaccgc
     3781 caatattttg actacgctag atcaaccggg ttggtattat tggttgatgt atggaagacc
     3841 gagtgtaaat atgcttaaac gttcgacgct tcaattttgc ggaattaatc ctgttcaagt
     3901 taattatttt ggaattgtgc gtaattcaaa tgctgcacaa agaaaaaaat ggcttcgtca
     3961 agctcggttg ttgggcgaga aattaaagta aagcgatttt taataaacct tatgcttgct
     4021 tgggcgtaga aatcgaaact gtacttatct tagcctttca atttgagcgt atgagccagt
     4081 cgataaaatt ccggattttc gtattgagtt tgggtgtttt tctttgtcag tttactcatg
     4141 caaatgcagc cacaaaaata gattcaggtc gtaaagacac gctcgaattt accgcagaag
     4201 atgcgattcg gtatgcgaag tttattaatt ttttacgttt ccgcgaagat tggactgccg
     4261 ctcgaactcc caatggagac gcttgggtgg tgactgctga acgcgattat ttgagcaaga
     4321 aaattattcg atctaccgga tcagagaatc aatatttcga taagaaaaaa ggtgccttag
     4381 tccgccgcca gcgcatgatg acgattcatg cccgaagcgg gaaaatatta ggtaaacaaa
     4441 cgaaagtcga gattcgccgg aatcaagcag aaggcacgaa ataattcctt tttagcattt
     4501 tgccgcagat taaacgtatg ccgcgccaag gatagaggtg agttcccacg aaccgatagc
     4561 ctgcccgggc gcccaagtaa taaattccag gacagtgtaa tcagcacgct tcgacaagct
     4621 cagcgcgcgg tagcagttta cctcaatcaa aacagcacat cagcaaacta gcacatcagc
     4681 acatcacaaa tagcacgctt cgacaagctc agcgcgcgga tttatacgct ccctgagctt
     4741 gtcgaaggga gcacaccaac aatactcgga atgaattccg atgctgcaag agtgaatatc
     4801 tatcattttc aaattttcaa atcgccacat cttcaaatca tccattatct tcgtcgcatg
     4861 aacatcaacc cacacgacga taagcgtctt ttcttattag acgcctttgc cttgatttac
     4921 cgagcttatt ttgcctttag ccggaatccg ctgatgaatt ctaaaggcat gaatgtgagt
     4981 gctgcatcgg gttttacgaa tacgctggtg gagctgatgc tgaaggaaaa acctacccat
     5041 attgcggtgg tatttgattc ggctgcggag actaatcgtg ccgtggaaca cagcttttac
     5101 aaggctcacc gcgaggcaat gcccgaagat attcagagca atttaccttg gattaaagag
     5161 ataattagtg catttaatat tccgatgctc gaattggctg gttatgaagc cgacgacatt
     5221 attggaacca tcgcaaaagt gaaggcgcgc gagggacatg tggtgtatat ggttacgccc
     5281 gataaggatt acggacagct ggttgagccc aatatttaca tgtacaaacc ggctcgtatg
     5341 ggcaacgata ttgagatttt aggtgttccg gaaatttgtg caaaatggga gattgatgat
     5401 ccattgaaag tgatcgatat tttgggtatg tggggcgatg cttcggataa cattccggga
     5461 attccaggaa ttggtgaaaa gactgccaag aagctcattc agcagtatgg ctcgatggaa
     5521 aatatgattg cccattcgca tgaattgaaa gggaaaatga aagaaaatgt ggaggcattt
     5581 gtcgatcagg cacatatttc gaaaatgctt gctaccatta ttttggatgt accaattgaa
     5641 gtgagcgatg aggatttatt aatatctgaa cccgataaag agaaattagc tgccttattt
     5701 accgagctcg aatttcgctc tatgggccgt cgaatcttgg gtgccgattt tagtgtgaat
     5761 cagcagatag cgcctgaagt taaaaagagt attcctgcag cacctaagcc atctaacgga
     5821 caaatttcta tgttcgggga ggaaaataat ccagaagaag atcatcccga accagccgaa
     5881 aaagggaaaa atatcagcaa tacaccgcat acgtattttc gtgtagaaag tgaggaagaa
     5941 ttaaaaaatc tggctgattt actcggacaa caagcggaat tttgttttga tactgaaacg
     6001 tcgtcgctcg attattttag tttggaatta gtcggacttt cttttagtgt aaaagaaggt
     6061 gaagcgtatt atgtcccaac gccttcggat aaggaagaag tgagccggat tctcagttat
     6121 ttcaagcctt tgctcgagag taaaaccatc cttaaaattg gacaaaacgt taaattcgat
     6181 ttgctcgttt tgcgtcagtt tggagtagaa gtttccttgc ccgtttatga taccatgtta
     6241 gcgcattatc tagttgagcc cgacatgaaa cacggaatgg attatttgag cgaaacctat
     6301 ttgggataca cgcctgtttc cattaccgaa ctcattggaa aacgcggaaa aaaccaagga
     6361 acgatgcgcg atgtgccggt ggagcaagtt gtagaatatg catccgaaga tgctgatatc
     6421 acgttgcaat taaagcatgt tattcagccc atggtggaga aaagagaagt ggtagatgta
     6481 ttgcagaaaa ttgaattgcc gcttattcct gttttagccg atatggaata cgaaggcgta
     6541 aaagtggatg aagcattttt agcgaattat tccgaagaat tacaaagcga tttaattctc
     6601 ttgcgcgatg cgatttggga aatggctgga caagaattca acatcgattc tcccaaacaa
     6661 ttaggggaaa tattgtttga taaattaaaa ttgcctggag ccgaaaaaac caaaacggga
     6721 caatttgcaa ctggtgaaga aattctcagc aaactcgaaa aggagcatcc gattgccgcg
     6781 aaactgctcg attaccgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngat
     6901 tatagtcaga ttgaattacg tttggtagct gaaattgcga aagaagaagc catgttggag
     6961 gcatttacca aaggcatcga cattcaccaa gcaacggctg cgcgcgtgta tggtgtagct
     7021 ctagaggatg tgacacggga aatgcggagc aaggcgaaaa tggtgaattt tggaattatt
     7081 tattcgatat cagcattcgg actttctcaa cgattaggcg ttccacgtaa agaagcggct
     7141 gaattaattg agaattattt tgcacaatat cctggaataa aaggttacat gagcagcacg
     7201 ctagaattcg ctcgtgccaa tggatacgtg aaaacgatga tggggcgtcg caggtatttg
     7261 aaagatatta attcacgtaa tttcaccatt cgtggatttg ccgaacgaga ggcaattaat
     7321 tcgcctgttc aaggttctgc tgccgattta attaaaatgg cgatgattgg cattcaccaa
     7381 gaattaaaag agaaaaattt acgctctaaa atgactttac aggtgcatga tgaattggta
     7441 tttgatgcac ataaagacga gctcgaaatt ttaaagccaa ttattctaca tcacatgcgt
     7501 acagcctttc aaaccaccgt tcctattgaa gtggaaatag gtacaggaat gaattggtta
     7561 gaagcacatt gatttaattt tcattcaaag attgtttgca aggatgattt tcttgttaag
     7621 gaatttttat gtcttgttta ttagtggttt aaatacgttt atacctgtaa ttgtgctttt
     7681 atattactat ttaaactcaa ctgataattt attgctatat ttagacttca tcccaacctt
     7741 gtttatatct gtttttggta atatttcact agcatttctt cagaaataaa tctgattgat
     7801 ttgaattgcc attatatgga taactcgcat gcaatctttc accaaaatct acccaatgat
     7861 gaagtatttc tacgcatgtc tttttcttct cgttagccag ttatccctac tcgctcagca
     7921 gaataccgat aatactcagc ctccacgctg ttttactgat gaatatttac gtgcaaaacg
     7981 cgaagctgat cccacgttta aaacgcgtga agccattcag aatattctca tccaacagca
     8041 agctcgtcct gctaatgatc gttcggtatt gactatacct gtggtaatac atgtggttta
     8101 ccttccaggg caagatccct atggacatta catcgatatc tataatggaa ttgatcatct
     8161 caatgctgct ttttcaaatt ctggacaata tcataatgca cttggggtaa ataccaatat
     8221 tcaattttgt cttgctacaa ctggaccttt aggtgaatcg acttatggga atcccattac
     8281 ttataccgaa tcggaacttt ccaacttaac cattgaaacg caagatgccg agttgaaaaa
     8341 cctcattc
//
LOCUS       DAOOQW010000089         8311 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_10604_length_8311_cov_5.901768, whole genome shotgun sequence.
ACCESSION   DAOOQW010000089 DAOOQW010000000
VERSION     DAOOQW010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 8311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8311
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10604_length_8311_cov_5.901768"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..920)
                     /locus_tag="PK281_06490"
     CDS             complement(<1..920)
                     /locus_tag="PK281_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014790134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HOY49030.1"
                     /translation="MSTLINDSIPAAFTAKDLVTDQDVRWCPGCGDYSILKQVQTVLP
                     ELGLKREEIVFISGIGCSSRFPYYMETYGMHSIHGRATAIASGLKATRPDLSVWIITG
                     DGDSLSIGGNHFIHLLRRNFDVNVLLFNNEIYGLTKGQYSPTSELGKVTKSSPMGSVD
                     HPFNPLALVMGADGTFVARSMDRDPIHMRTILKRAHAHKGTSLVEIYQNCNVFNDGAF
                     EVFTEKSSKADETIMVEHGQPLIFGAQKDKGILLDGYTPRVVSLNDVSANDLWIHDEH
                     NRAKANLLVRFFDDASVEGHFPRPFGVFYS"
     gene            complement(922..2766)
                     /locus_tag="PK281_06495"
     CDS             complement(922..2766)
                     /locus_tag="PK281_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HOY49031.1"
                     /translation="MTNDSLIATDATILFAGDSGDGIQLTGDQFTTTTALFGNDVSTF
                     PNFPAEIRAPQGTLAGVSGFQIKFGSEEVYTPDDNCDLLVVMNAAALKANLKQLKAGG
                     MIIANTEGFDGKNLRLAKFPEGVNPLEDGSLDSYKLYTFDVTKMTKATLEGSGLGTKE
                     IDRTKNMFVLGFIYWIYNRPLEHTIKFLEQKFAKRPEIVEANIKTLKAGYHYGDTSET
                     FMQRVQVKPASIEPGIYRNINGNPATAIGLIAAAQKSGLPLFLGSYPITPASDILHEL
                     SKHKNFGVRTFQAEDEIAAITSSIGAAFGGSLAVTTSSGPGIALKTEAMGLAIMLELP
                     LVVINVQRGGPSTGLPTKTEQADLLQAVYGRNGEAPMPVIAAQTPSDCFYAVYEACRI
                     AVEHSTPVFFLSDGYLANGAEPWKFPTSADLPSITARFATEEDRIEGKFLPYKRDEKL
                     ARKWAVPGVKGLEHRIGGLEKENETGNISYDPDNHEFMVKLRAEKVNRIADTIPLQTL
                     DNGPEKGKLLIVGWGSTYGSIKTAVKRALEEGYSVAHAHVRYIHPFPKNLGELMKNYD
                     HVLVPEMNNGQLVRLLRDQYLVPAEGLNKIKGIPFTSEELYTKITTLA"
     assembly_gap    2837..2936
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3006..5159)
                     /locus_tag="PK281_06500"
     CDS             complement(3006..5159)
                     /locus_tag="PK281_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006987193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HOY49032.1"
                     /translation="MRNHLLICLSFTALIACNSSTEMTRKPYEWPDAKAPIAEKKEHL
                     RIIHGDTVRDDYYWMIDYFKKGPDSTKVVDYLKAENAYLETMMAGTKKFQENLFTEMK
                     ARIKEKDESVPYFNKGYFYYYKTDEGKEYFKFCRRKGSMEGAEEVLLDADKMAEGKSY
                     FSLDAMEVSPDNSMIAYAVDEVSRRQYTIHVKNLATGETYSDQIAGTSGNLCWANDNK
                     TIFYTSNNPVTLLSEKIKRHTLGSDAKTDVVVYEEKDKTNYIGVDKSKDDQHIMIYSS
                     ATMSSEIYLLDANKPTGAFRSFQARMKDVLYYVTPLADRFLITTNWNAKNFRLMECPL
                     DKTGSDNWKEVIGNRNDVLLQGVAEFKDFIVLSERKDGLVKLRVRSLKDGKEHYVDFG
                     EAAYMANFSTNVDYSSSVLRYSYTSMTTPGSVFDYDMNTQERKLLKQQEVVGGFDPKN
                     YVTERIYAKAKDGTRVPISLVYKQGYKKDGTAPLLLYAYGSYGNSTDAGFNSNRLSLL
                     NRGFVFAIAHIRGGQEMGRQWYEDGKLMKKKNTFTDFIDCGEFLIQEKYTSKGHVYAQ
                     GGSAGGLLMGAIANMAPDLWNGVIAQVPFVDVVNTMLDESIPLTTNEFDEWGNPTNKD
                     AYEYMKSYSPYENVEKKNYPNMLVATGLHDSQVQYFEPAKWVSKLRAMKTDKNVLFLH
                     TNMEFGHGGASGRFDYLKDVALNYAFLFSLEGIVE"
     gene            5301..6278
                     /locus_tag="PK281_06505"
     CDS             5301..6278
                     /locus_tag="PK281_06505"
                     /inference="COORDINATES: protein motif:HMM:TIGR01293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOY49033.1"
                     /translation="MEYRRLGRSGLQLSALSLGSWLTFGKQISDDVAEELMVTAYENG
                     VNFFDNAEIYARGKSEIVMGNILKKQDWRRDSYVVSSKVFWGGDKPNQTGLSRKHVFE
                     ACHAALKRFQLDYLDLFFCHRPDPNTPMEETVWVMNDLIRQGKILYWGTSEWSSQQIM
                     EAFHIARREHLIPPTMEQPQYNLLHRDRFEVEYERIFSEMGYGSTIWSPLASGILSGK
                     YNTGFPEGTRLGMQGLEWLRDKMHTDENLHIARELTVLAQDLGTTLPQLSIAWCLKNK
                     NVSTVILGASRKDHLIDNFKALDVVPLLTEEVIQQIENTVKNKPTPAGF"
     gene            6364..>8311
                     /locus_tag="PK281_06510"
     CDS             6364..>8311
                     /locus_tag="PK281_06510"
                     /inference="COORDINATES: protein motif:HMM:NF013360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016641 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, oxygen as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysyl oxidase family protein"
                     /protein_id="HOY49034.1"
                     /translation="MKHVLRAPYFLFCLLALSALFSSNLQAQCTSPLIPVTVIINPDN
                     YPQEITWSLSSGGTSLATGTSTGTTVCVNPETCVSFTINDGAGDGICCGYGNGSYYIV
                     LAGDTVATGGNYGAGQTVEFNCPPGGDCISAIPTTTGSHTAPQRNTWYTFTPDSSGMY
                     FISTCVINTCNTKLWVYDHCAGLLVTNTNEGTIYYDDNAGGCGDQARVNALMEAGHTY
                     YIRIGDSQQDCAGEINWTLNYNGPVVGCMDPTACNFNPVASVPGVCYLDGDPNCPEGR
                     PDLIVVENTIKTSIYLSTIEAATCSVVEGCLTGYGTRDLIRFTTHIKNIGTSDYYIGS
                     PTTQPSQFSFGNCHGHAHYSGYAEYIMYNEAGTAIPVGFKNGFCVLDLECGDGGTAQF
                     GCSNMGISRQCGDIYNSGLDCQWMDITEIDTGRYTMVVRVNWDNSPDAMGRVELSHTN
                     NWAQVCIRLGRDQNGARTIALLDDCEPFIDCNGEVYGNSKLDCEGNCGGMRQMGDVNL
                     SGSQEMADAHKYVTDILGYDMPVTACNDINQDGKITVFEASLISSCVNYGVNHPHTGN
                     LPHNHCNFPGGLYNPGDTVTLSILNVDFSEKFVDIGILNPDSRVNAYEFDMHGLNIWN
                     VETLIPAGEYNAQPEFLIGGQKVIS"
BASE COUNT         2313 a         1894 c         1660 g         2344 t
ORIGIN      
        1 tctgagtaaa acacaccaaa cggacgagga aaatggcctt ctaccgatgc gtcatcaaag
       61 aaacgcacca acaaattggc ttttgcacgg ttgtgctcat cgtgaatcca gaggtcgttt
      121 gccgaaacat cattcaacga aaccacacgc ggagtatagc catcgagtaa tatacccttg
      181 tctttttgtg ctccgaaaat tagcggctgt ccatgttcaa ccattatcgt ttcatcagcc
      241 ttgctcgatt tctcagtaaa gacttcaaac gcgccatcgt taaacacatt acagttttgg
      301 taaatctcca ccaaggaagt tcccttatga gcatgtgcac gcttaagaat agtacgcatg
      361 tgaatcgggt cgcgatccat tgagcgagct acaaaagtcc cgtcagcgcc catcaccaat
      421 gccaatggat tgaatggatg atccaccgaa cccataggtg aggatttagt cacttttcct
      481 aattccgaag tcggtgaata ctgacctttc gttaagccgt aaatctcgtt gttaaacaac
      541 aatacgttca cgtcaaaatt gcggcgtaac aggtgaatga agtggtttcc tccaatcgaa
      601 agcgaatcgc catcaccggt aataatccac acacttaaat cggggcgagt tgctttcaat
      661 cccgatgcaa tggcagtagc acgaccatga atagagtgca taccatacgt ttccatgtaa
      721 tatggaaagc gcgaggagca accaattcca gaaatgaaaa caatttcttc gcgtttcaat
      781 cccaattctg gaagtacggt ttgtacctgc ttcaaaatag aataatcgcc acaacccgga
      841 caccaacgta cgtcctgatc ggtcactaaa tcttttgcgg taaatgccgc gggaatgcta
      901 tcgttaatga gcgtgctcat atcaggcaag tgttgtgatt ttggtataga gttcttccga
      961 agtaaatgga atacccttga ttttattcag cccttcggca ggaacgaggt attggtcgcg
     1021 cagcaaacga accaattgtc cgttgttcat ttccggcacc aacacatggt cataattttt
     1081 catcagctcg cccaagtttt taggaaatgg atgaatgtaa cgaacatgcg catgagcaac
     1141 cgaatagcct tcttctaacg cacgttttac cgccgtttta atcgagccat acgtcgagcc
     1201 ccagccaaca atcagaagtt ttcctttttc aggaccattg tcgagcgttt gcaaaggaat
     1261 ggtatcggca atgcggttca ccttttcggc gcgcagctta accataaact cgtggttatc
     1321 gggatcgtaa ctgatgttcc ctgtttcgtt ttctttctcc aaaccaccaa tacgatgctc
     1381 taggcctttc acgcccggaa cagcccattt acgcgctagt ttttcatcgc gtttgtaagg
     1441 caaaaatttc ccttcaatgc gatcttcttc tgtggcaaaa cgggccgtaa tgcttggcaa
     1501 atcggccgat gttggaaact tccaaggctc agcaccattc gctaaatatc catcactcaa
     1561 gaaaaatacg ggtgtgctat gctccaccgc aatgcgacac gcttcgtaca ctgcataaaa
     1621 acaatccgaa ggtgtttgag cagcaatgac tggcatcggc gcttcgccat ttctaccata
     1681 aactgcttgt aataaatcgg cttgctcggt tttcgtaggt aatccggttg atggtccgcc
     1741 acgctgcaca ttgataacaa ccaagggcaa ttcaagcata atggccaatc ccatcgcttc
     1801 ggttttcaag gcaatgcccg gtcccgaaga ggttgtaaca gctaatgatc cgccaaatgc
     1861 agctccaata gatgaggtaa tggcagcaat ttcatcttcc gcctgaaacg tgcgaacacc
     1921 aaagtttttg tgcttcgaca attcgtgcaa tatatccgat gcgggcgtaa ttggatatga
     1981 tcctagaaac aagggtaatc ccgatttctg agcagcagca atcaatccaa tagctgtggc
     2041 aggatttccg ttaatgttgc ggtaaatacc gggctcaatg ctagcgggct ttacctgcac
     2101 acgttgcatg aaggtttcgc tcgtatcgcc gtaatgataa ccagccttga gggttttgat
     2161 gttcgcctca acaatctcgg gacgtttcgc aaacttctgc tccaagaatt taattgtatg
     2221 ctctagcgga cgattgtaaa tccagtagat aaacccaagc acaaacatgt tcttggtacg
     2281 atcaatttct ttggtgccca aaccgctgcc ttccaatgtg gctttggtca ttttggtcac
     2341 gtcaaacgtg tagagtttat agctgtcgag cgagccatct tcgagcggat taacgccttc
     2401 gggaaacttc gctaaacgta ggtttttacc atcaaagcct tcggtgttgg cgataatcat
     2461 tcctccggct ttcagttgtt ttaggttggc tttcaaagcc gcagcattca tcaccaccaa
     2521 gaggtcgcaa ttgtcgtcgg gagtgtaaac ttcttccgaa ccaaatttta tctgaaatcc
     2581 cgaaactccc gccaatgtgc cctgtggagc acgaatctca gcggggaaat taggaaaggt
     2641 acttacgtcg ttaccaaaaa gtgcggtggt cgtggtaaac tgatcacctg taagttgaat
     2701 tccatcgcct gaatcaccag caaacaatat cgttgcgtcg gtcgcgatga gggagtcgtt
     2761 tgtcattgca aaaagagaac ggtcagagcc gtaaaatgtt gggcaaaggt agacaaaatg
     2821 catccgctat ctgcatnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngggc
     2941 tttcggctcg tgggaactcg cctctatccc ttttgcagaa tttcgtatat cgctatagcg
     3001 ttggattatt ctacaatccc ttctaacgaa aacaagaagg cataattcaa ggcaacatct
     3061 ttcaaataat caaatcgtcc cgatgcgcca ccatgcccaa attccatatt ggtatgcaaa
     3121 aacaacacgt tcttatcggt tttcatagcg cgtagtttgg acacccattt agcgggttca
     3181 aaatactgaa cttgactatc gtgaaggcct gttgctacca gcatatttgg gtaattcttc
     3241 ttctcaacat tttcgtaagg tgaatagctt ttcatgtact cgtaagcatc cttgtttgtt
     3301 ggatttcccc attcatcaaa ctcattcgtt gtaagtggaa tgctttcatc cagcatcgta
     3361 ttcaccacat ccacaaacgg aacttgagca ataacaccat tccataaatc aggagccata
     3421 ttcgcaatag cgcccatcaa caaacctcca gcacttccac cttgcgcgta aacatgtcct
     3481 ttggatgtgt atttctcttg aatcaaaaat tcaccacaat caataaaatc ggtaaaggtg
     3541 tttttcttct tcatcaattt cccgtcttcg taccattggc gccccatttc ttgtccgcca
     3601 cgaatgtgag caatggcgaa tacaaatcca cggtttaaca aactcaaccg gtttgagtta
     3661 aatcctgcat ccgtgctatt tccgtaagaa ccataagcat aaagcaacaa tggagctgtt
     3721 ccatctttct tataaccttg cttgtaaacc aacgaaatcg gaacgcgtgt accatctttt
     3781 gctttggcat aaatacgctc ggtcacatag ttttttggat caaatccacc cacaacttct
     3841 tgctgcttca agagcttccg ttcttgcgta ttcatgtcgt agtcgaacac cgaaccagga
     3901 gttgtcatgg atgtgtatga atatcgaagc accgaactcg aataatccac attggtagag
     3961 aaattcgcca tgtaagctgc ttcaccaaaa tctacgtaat gctctttccc atctttcaag
     4021 cttctcacac gtagttttac taatccatcc ttccgctcgc tcaacactat aaaatctttg
     4081 aattctgcca cgccctgaag caacacatcg ttacgattgc caataacttc cttccaatta
     4141 tccgaacctg ttttgtctaa tggacattcc attaaacgga agtttttcgc attccagttc
     4201 gtagtaatta aaaatcgatc agccaaagga gttacataat agagcacatc tttcatgcgt
     4261 gcttgaaacg aacgaaacgc tccggttggt ttattcgcat ccaacaaata aatctccgac
     4321 gacatcgtag cactcgaata tatcataatg tgttggtcat ctttcgactt atccacaccg
     4381 atataattgg ttttatcttt ctcttcgtaa acaactacat cggtttttgc atcgctgcca
     4441 agtgtgtggc gtttaatctt ctccgaaagc agtgtaaccg gattgttcga agtgtagaaa
     4501 atcgttttgt tatcattcgc ccagcataaa ttccccgatg ttccagctat ttgatccgaa
     4561 tatgtttctc ctgtggccaa attcttcacg tgaatcgtat actgtcggcg cgaaacttcg
     4621 tctacagcat aagcaatcat cgaattatcc ggacttactt ccatagcatc caaagaaaaa
     4681 tacgatttgc cttcagccat tttatctgca tctaatagaa cttcctccgc tccttccatg
     4741 ctgccttttc gacgacaaaa cttaaagtat tctttgccct catcggtctt gtaataataa
     4801 aaatagccct tattgaaata cggtaccgat tcgtcctttt ccttgatacg agccttcatt
     4861 tcagtaaata aattctcctg aaactttttc gtcccagcca tcatggtttc taaataggca
     4921 ttctccgcct ttaggtaatc caccaccttg gtgctatccg gacctttttt gaaataatca
     4981 atcatccaat aataatcatc ccgaacagtg tcgccatgaa taattcgaag atgttctttt
     5041 ttctctgcta taggtgcttt cgcatccggc cattcataag gtttacgtgt catttcggta
     5101 gatgaattac aggcaattaa ggctgtaaaa gaaagacaaa tcagtagatg attacgcata
     5161 gaataatttt gaaaattaaa ttggtagtcg gtaaatgtac tcggcaattt tccaattttt
     5221 aggtttacgg gattaaatca aacattttcg acgtaattgc attattacgc ttagcaattt
     5281 aacactagaa aaataaaatc atggaatatc gtcgtttagg ccgttctggt ctgcaactat
     5341 ccgctttatc attaggttct tggctcactt tcggcaaaca aatcagcgat gatgtagcag
     5401 aagaactcat ggtcaccgcc tacgaaaatg gagttaactt tttcgacaat gccgaaatct
     5461 acgcacgtgg aaaaagcgaa atcgtcatgg gtaacatcct caaaaaacag gattggcgac
     5521 gcgattccta tgtggtttca agcaaggtgt tttggggcgg cgataaaccc aaccaaaccg
     5581 gattgtcgcg taaacacgtg ttcgaagctt gtcatgcggc attaaagcgc ttccaactcg
     5641 attacctcga tttatttttc tgtcatcgtc ccgatccgaa tacgcccatg gaagaaaccg
     5701 tttgggtgat gaacgacctt atccgccagg gaaaaattct ctattgggga acttccgaat
     5761 ggagctctca acaaatcatg gaggcatttc acattgcccg ccgcgaacat ttgattccgc
     5821 caaccatgga acaaccgcaa tataacttgc tacatcgcga tcgtttcgag gtagaatacg
     5881 aacgcatctt ttccgaaatg ggctatggat caacaatctg gtctccatta gcatctggaa
     5941 ttttatccgg taaatacaac acgggatttc ctgaaggaac gcggctcggc atgcaaggct
     6001 tagaatggct tcgcgataaa atgcataccg acgaaaacct gcacattgct cgcgaactaa
     6061 cggtattggc tcaagatctc ggcacaacac ttccgcagtt atccatcgct tggtgcttga
     6121 aaaataaaaa cgtgagcacg gtaattcttg gcgcttcccg taaagatcat ttaatcgaca
     6181 atttcaaagc actagatgtg gttccattgc tcacagaaga agtaattcag caaattgaaa
     6241 ataccgtaaa aaacaagccg acaccagcag gtttttaacc aatatcgcat ctggggcttg
     6301 gaattagtta taaatgacta attttaagcc cttcgtgttt aaaatccaca aatacatctc
     6361 taaatgaagc acgttttacg cgcaccttat tttctgtttt gtttacttgc attgagcgca
     6421 ctattttcaa gtaatcttca ggcacaatgc acaagtccgc taattccggt aaccgtcatc
     6481 atcaatcccg acaactatcc tcaggaaata acctggagcc ttagtagtgg tggaacatca
     6541 ctagcaactg gaacatcaac cggaacaacc gtttgtgtaa acccagaaac ctgcgtttcg
     6601 tttactatca atgatggagc gggcgatggt atttgctgcg gttatggtaa cggttcttac
     6661 tacatcgttt tagctggaga taccgttgct acaggcggaa attatggagc aggccaaaca
     6721 gtagaattta actgtcctcc cggtggcgat tgtatttcag ctatcccgac aacaacaggt
     6781 agtcacacag ctccacaacg caatacgtgg tacacattta ctccagattc ttcaggaatg
     6841 tatttcattt caacttgcgt aatcaatacg tgtaacacca agctttgggt atacgatcat
     6901 tgtgctggtt tacttgtaac gaacaccaac gaaggaacca tttattacga cgataatgct
     6961 ggtggttgcg gagatcaagc tcgcgttaat gctttaatgg aagctggcca cacatactac
     7021 attcgaattg gagattctca acaagattgt gctggagaaa ttaattggac tttaaactat
     7081 aacggtcctg ttgtaggttg tatggatcca acagcctgta atttcaatcc agttgcttcg
     7141 gttccgggag tttgttattt agatggagat ccaaattgtc ctgaaggtcg ccccgattta
     7201 attgtggttg aaaacaccat taaaacatcc atttatcttt ccacgattga ggctgcaact
     7261 tgttctgtag ttgaaggatg tttaacaggt tacggcacgc gcgatctgat acgtttcaca
     7321 acacacatca aaaacattgg aacgtccgat tattacatcg gtagcccaac aactcaacca
     7381 tctcaatttt cttttggtaa ctgtcacggt cacgcacatt atagcggtta tgccgaatac
     7441 atcatgtata acgaagcagg tacagctatt ccagttggtt tcaaaaatgg tttctgcgtt
     7501 ctcgacttag aatgtggcga tggcggtact gctcagtttg gatgttctaa catgggtatt
     7561 tctcgtcaat gtggcgatat ttataactct ggtcttgatt gtcagtggat ggatattacc
     7621 gaaatcgaca ctgggcgcta taccatggtg gttcgtgtaa actgggataa ttctcctgat
     7681 gcaatgggtc gtgttgaatt gtcacacacg aataactggg ctcaagtatg tattcgttta
     7741 ggaagagatc aaaatggggc acgtacaatt gctcttctcg acgattgcga accatttatc
     7801 gattgtaacg gagaggttta tgggaactcc aaactcgatt gtgaaggaaa ctgcggcgga
     7861 atgcgccaaa tgggagatgt gaatctttct ggctcacaag aaatggctga tgcacataaa
     7921 tacgtaacag acattttagg ttacgacatg ccagttaccg cttgtaatga tatcaatcaa
     7981 gatgggaaaa tcaccgtgtt tgaagcatct ttgatttcaa gctgtgtgaa ttatggtgtt
     8041 aatcacccac atacagggaa cttaccacat aaccattgca acttccctgg tggattatac
     8101 aatccaggag atactgttac tctttctatt ttgaatgttg atttctcaga aaagttcgta
     8161 gacatcggaa tcttaaatcc tgattcacgt gttaatgcat acgaatttga tatgcatgga
     8221 ttaaatatct ggaatgtaga aacattaatc cctgctggag aatataatgc tcaaccagag
     8281 tttttaattg gcggacaaaa agtaatcagc a
//
LOCUS       DAOOQW010000090         8299 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_10622_length_8299_cov_6.128093, whole genome shotgun sequence.
ACCESSION   DAOOQW010000090 DAOOQW010000000
VERSION     DAOOQW010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 8299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8299
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10622_length_8299_cov_6.128093"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(247..1005)
                     /locus_tag="PK281_06515"
     CDS             complement(247..1005)
                     /locus_tag="PK281_06515"
                     /inference="COORDINATES: protein motif:HMM:NF024902.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOY49035.1"
                     /translation="MRKILLLIACYFLVLSAQALSPASLFAEAEQAYIAGNYKTAIIK
                     YEQILSEQKAANAELYFNLGNAYYKLTDYPNAILSYERALRLEPTDEDVRFNLALANQ
                     RIEDKIEAVPELFYKRWFVSIRSLAGADAWGILFLIALSFCAASFFLFVLGKTVAMRK
                     IGFYAGLFTFLISLLFLGLGISMYSIQNTHSEAIVFSGSVSVKSSPVESGTGLFVIHS
                     GTKVSLTDELGDWVKIRLADGNVGWVQLGDLEKI"
     gene            complement(1002..2822)
                     /locus_tag="PK281_06520"
     CDS             complement(1002..2822)
                     /locus_tag="PK281_06520"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HOY49036.1"
                     /translation="MKRIILLGFFIQCVLFMQAQEVRFNGSASKTKVAVGEQFRLTYS
                     INTSASGFNGPDLSAFDVYSGPNQNTSMQIINNAVSQTLSYYYILAPRKEGKFTFTPA
                     QINVANGRVRSNEVSIEVVKGNPAQTTNPNPANPRQNVNPGTNQPSTAGASDKLFIKA
                     ILNKSSAFLDEQVEVTYKIYSKYGQINFSDLKLPTFNGFYTEDIKSQNNDKLEVENYN
                     GSQYYTAEIKKTLLFPQKSGKLEIPALDATCLVRERVNSQSFFDQFFGGGYRDVSVKV
                     KSQPMSIQVFAHPGSARPADFNGAVGDFTVTAEIDKDKVRSGDPVNLKFTISGKGNLK
                     LLEAPALTFPPEFEGYDPQIKDQIRVTEAGMSGSRTFEYLLIPRAGGEYVLGPFTYTY
                     FSPTKKNYVTTTLPRMLIHVDKTVGDQTNVARSGSGTAPKQLATDIRFNKKNTHAFEP
                     IDEHPFFLSGGFYALTALPPLSLLLLLFMRRRQADRSANSGLYRMKEAGSMAQKRLKK
                     ADELLKADKKDAFYEEVYRALYGYLSDKLQIPAAELARPKIMQELGKRQVPEFDCNEL
                     IRLLDACEFARFAPGASSGMQQVFTDSVSLLTRTEKYLKS"
     gene            complement(2835..3500)
                     /locus_tag="PK281_06525"
     CDS             complement(2835..3500)
                     /locus_tag="PK281_06525"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOY49037.1"
                     /translation="MKQLSLIYLLCVSALGFSQSEASHFRKGNELFKAGKFNDAEIEY
                     RKGLAKQDKSWTGKYNLADALYKQDKFKEAEVILDSLSKGTKNQKQLSSVYHNLGNAQ
                     LKAKDYEKSVESFKKALKLDPQAEDSRYNLSYALQKLRQQQQQQQQQQQNKDQKQQQQ
                     QKQQQQQQQPQDKKQEEKKQNINQQEAERMLDALDRDEKELRKKTEKRESRNGQGGSG
                     KDW"
     gene            complement(3497..4543)
                     /locus_tag="PK281_06530"
     CDS             complement(3497..4543)
                     /locus_tag="PK281_06530"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOY49038.1"
                     /translation="MFRFAYPFFLWGLAVLPVFGLLFAARMSWKRKAIKRFGDPALME
                     VLMPDRSQGKIRTKFFLLVGAWICLIIGLANPQIGSKYEEVKRQGVDLIIALDVSNSM
                     LAEDIRPNRLERSKQAIIKLLDKLHDDRIGIVIFAGDAYLQLPFTNDYAAARMFLSTV
                     SNESIQYQGTAIGAAIQMGVKSLPRGGIRNKAIIVISDGENHEDDALAEAKAAKEAGV
                     IVHTLGIGTPDGSPIPEMRDGERIGFKTDDAGQTVISRMNPQMLQEIAQAGGGKFVQA
                     SGSDIGLEELFNQINSMQKSDFGTKAFTDYEDRFQNFLVLALMILLVEFLVDERRSAI
                     SRKMNLFGTKRKKQ"
     gene            complement(4547..5578)
                     /locus_tag="PK281_06535"
     CDS             complement(4547..5578)
                     /locus_tag="PK281_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013550042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOY49039.1"
                     /translation="MNEWNGLRLPPIDEITFAHPWALWFLLIVPIMVLWYIFKGRKGK
                     PELQMATLAPFVGRKPSAVVRLMHLPFILRCFTVALIITALCRPQTKLAWQDMTTEGI
                     DIMLAMDLSPSMLARDFKPNRLEAAKNVGIDFIDGRPNDRIGLVVFSGESFTQCPLTV
                     DHSVLKNLLTVAEPDFLADGTAIGMGLASAVKRLKDSKSASKVIILITDGENNAGSVS
                     PLTAAEIAQIYGIRVYTIGVGTRGRAYSPAYRYPNGEFAFDYVEVRIDEPLLEKIAKM
                     TDGQYFRATNNEALKEIYQKIDLLEKTRFDVTEFRRKREEFWPLALLAALFLFTEWLL
                     RNTLMRSIP"
     gene            complement(5571..6527)
                     /locus_tag="PK281_06540"
     CDS             complement(5571..6527)
                     /locus_tag="PK281_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49040.1"
                     /translation="MKKTISFCFALLLACSSLWAQQASVKSDRYRIRLGEPFQLTIKA
                     IVEDGQRVVWPEVTDTLGGHFDILKRGPVDTVADADRKGMELLQQIEATSFDSGMHTM
                     PVFAFDIINGKGDTVHIVSDPLTFQVLTVPVDTTKAIKDIHGVVDVPFDIGEYIPWIL
                     VGLLVAALLVAGIYLWLKRKRPEAPKAPEAPKTPAWERALAALNEIGKEKAWQSGREK
                     LYHSAITDTIRAYIEEQFQLPAMESTTDETLHMFRKVVTDTNTNQTLKQILILADLVK
                     FAKERPLPAEHERSLELARNFVELTRPQEIIQPETGKEETHE"
     gene            complement(6524..7396)
                     /locus_tag="PK281_06545"
     CDS             complement(6524..7396)
                     /locus_tag="PK281_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOY49041.1"
                     /translation="MEQKELLKKVRKLEIKSRGLSAQLFSGQYHSAFKGRGMSFSEVR
                     EYQPGDDVRTIDWNVSARLNHPYVKVYEEERELTIMLVVDVSGSKDFGTQVQFKMELV
                     TEVCAVLAFSAMQNNDKIGVIFFSDKIEKYIPPKKGKSHILRIIRELIDFRPENRLTN
                     IDQALKFLTNVIRKRCTAFVLSDFYGKGYEDTLKIANKKHDIVALRVTDPREISLPDV
                     GLVRFRDAETGEAIWVDTSDRAVRKSYEIHYKKHEDFVKSTLSKAGVDQAVLHTDRSY
                     IQPLSNLFKRRGTR"
     gene            complement(7470..7829)
                     /locus_tag="PK281_06550"
     CDS             complement(7470..7829)
                     /locus_tag="PK281_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015781902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HOY49042.1"
                     /translation="MKTAPNTIVQLTEEFALEAIQFSRILKKEDQRELAFQLFKSGTS
                     IGANVAEAQHAESKLDFIHKLKIASKEARETAYWLNLCRRSQYLPGNPDAESKLLSIE
                     KMLSKIISTSKKSLNPK"
     gene            complement(7994..>8299)
                     /locus_tag="PK281_06555"
     CDS             complement(7994..>8299)
                     /locus_tag="PK281_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002671404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HOY49043.1"
                     /translation="EKIEKYIVDIVFATRFPKDHNLAKYQPLISFGASPRASISLALA
                     AKAYAFIKRRGYVIPEDIRAVSMDVLRHRIGLTYEAEAENITTENIISEILNVVEVP"
BASE COUNT         2336 a         1796 c         1673 g         2494 t
ORIGIN      
        1 tcaaattaaa aagggtgaaa aacacgatct tattataaaa tagatgaatg aaagtatgta
       61 attataaaaa actaccgatt tgaattacca aatcagaatt agatattttt aatattacta
      121 tttattgaat taaaatagct attgaatgta atcatcggaa aggatagctt caccagcttt
      181 cgctatagct acagctgatg aaatgaatag aagcaataag tctgctcggc tgcaaaaaaa
      241 ggaaaactaa attttctcta aatcacctaa ttgaacccaa cctacatttc catcggctag
      301 acggattttt acccagtcgc ctaactcgtc ggtgaggctt actttggtgc ccgaatgtat
      361 gacaaacaag cctgtaccgc tttctaccgg tgagctttta accgatacgc tacctgaaaa
      421 aacaattgct tcggaatggg tattttggat ggaatacata gaaattccta aacccagaaa
      481 tagcaaggaa ataaggaagg tgaacaatcc agcgtaaaac ccgattttgc gcatagcgac
      541 tgttttgccc aagacaaaca aaaagaaaga ggcagcacaa aacgataaag caataaggaa
      601 taaaattccc caggcatcag cgccggcaag actgcgaata gatacaaacc agcgtttgta
      661 aaacagctca ggaacagctt ctattttatc ttcgatacgt tgattagcta gcgccaaatt
      721 gaatcgaacg tcttcatctg ttggttctaa acgcaatgcg cgttcataac tgagaatggc
      781 gttcggataa tcagtgagtt tgtaataggc atttccgaga ttgaaataca attcagcatt
      841 cgcggctttt tgttcagata aaatctgctc gtatttgata atagccgttt tgtaattccc
      901 agcaatatat gcttgctcag cttcggcaaa taaagaggct ggcgataatg cctgtgcaga
      961 caaaacaaga aaataacagg caatcaggag gaggattttt ctcatgactt caggtatttt
     1021 tcggtacgtg ttaataggga aacggaatcg gtaaaaactt gctgcatacc tgaactagca
     1081 cctggtgcaa aacgtgcaaa ttcgcatgca tcgagcaatc tgattaattc gttgcaatca
     1141 aattcaggca cttgtcgttt gccgagttct tgcatgattt taggtcgagc taattcggct
     1201 gcaggtattt gtaatttatc actcaaatac ccatacaaag cccgatatac ttcttcgtaa
     1261 aacgcatctt tcttgtcggc cttcaatagc tcatcggctt tcttcaaacg cttttgtgcc
     1321 atgctaccag cttctttcat gcgatataaa cccgaattag ctgaacggtc ggcttggcga
     1381 cgacgcatga ataacaaaag caataaactc aatggtggca aggctgttaa tgcataaaat
     1441 ccacccgata aaaagaaggg atgctcatcg ataggctcaa atgcatgcgt attcttttta
     1501 ttaaatcgaa tatcggtcgc taattgttta ggtgccgttc ccgaacccga acgagctaca
     1561 ttggtttgat ctcccaccgt tttatctaca tgaataagca tgcgcggaag tgtagtggtc
     1621 acataatttt tcttggttgg agaaaaatac gtgtaggtaa atggtcctaa aacatattct
     1681 ccacctgcgc gaggaataag taaatactca aatgtacgcg aacccgacat acccgcttct
     1741 gtcactcgaa tttggtcttt tatctgtgga tcatatccct caaattcggg cggaaatgta
     1801 agtgcaggag cttctaacaa tttcaaattc ccttttcccg aaatggtgaa ttttaagtta
     1861 actggatcgc ccgaacgaac tttatcttta tcaatttcag cagtaacagt aaaatctcct
     1921 acagcaccat taaaatcggc tggtcgagct gaacccggat gtgcaaaaac ctggatgctc
     1981 atcggctgac ttttcacttt cacactcaca tcgcgatatc caccaccaaa aaactgatca
     2041 aagaagctct gagaattcac gcgttcacgg actaaacagg ttgcatccaa agcaggaatt
     2101 tcgagttttc cacttttctg cggaaagagt aaagttttct tgatttcggc tgtgtaatac
     2161 tgactgccat tgtaattctc tacctcgagt ttatcgttgt tttgactctt aatatcctcg
     2221 gtgtagaagc cgttgaaagt gggcagtttg aggtctgaaa agttgatttg accgtacttg
     2281 gagtaaatct tatatgtcac ctcaacttgc tcatctaaaa aggcactcga tttattcagg
     2341 attgctttaa taaatagttt atcgctagca cctgcagttg aaggttgatt tgttcctgga
     2401 tttacgtttt gacgcggatt agcaggattg ggattggttg tttgtgctgg atttcctttt
     2461 actacctcaa tacttacttc attcgaacgc acacgtccgt tagcaacatt gatttgagct
     2521 ggagtaaaag tgaattttcc ttctttgcgc ggagcgagaa tgtagtaata gctgagcgtt
     2581 tgagacaccg cattattgat gatttgcatg ctggtgtttt ggtttggacc agaatacaca
     2641 tcgaatgcac tcaaatcggg accattaaat cccgaagcgc ttgtattgat ggagtatgtg
     2701 agacgaaatt gctctccaac tgccactttt gttttggatg cacttccatt aaaacgcact
     2761 tcctgcgctt gcatgaatag cacacattga atgaaaaaac caagtaaaat tattcgtttc
     2821 atggcttgat aatattacca gtctttgccg ctaccacctt gtccgttgcg gctttcgcgt
     2881 ttttctgttt ttttcctaag ttctttttca tcccgatcaa gggcatctaa catacgctca
     2941 gcttcctgct gattgatatt ttgctttttc tcttcctgct tcttgtcttg cggctgttgt
     3001 tgttgctgtt gctgcttttg ttgttgctgc tgtttttgat ctttattttg ttgttgttgc
     3061 tgctgttgct gctgctgctg acgaagtttt tgtaaagcat aagacaaatt ataccggctg
     3121 tcttccgctt gtggatcgag tttcagggct tttttgaacg attcaacact cttttcgtaa
     3181 tctttcgctt tgagctgtgc attacccaag ttgtgatata ccgacgaaag ttgtttttgg
     3241 tttttagtgc ctttgctaag tgaatcaaga atgacttcgg cttccttgaa tttatcttgc
     3301 ttatacaagg catctgctaa attgtattta cctgtccaag atttatcctg cttagccaaa
     3361 cctttgcggt attcaatttc tgcatcgttg aattttccag ctttaaacaa ttcattcccc
     3421 ttacggaaat gactggcttc actttgcgaa aatcccaaag ccgacacaca cagcaaataa
     3481 attaaactaa gctgcttcat tgttttttac gtttagttcc gaacaaattc atcttgcgag
     3541 aaatcgcgct acgacgctca tccaccagaa attcgactaa taaaatcatc aaagctaaaa
     3601 ccaagaaatt ctgaaaacga tcttcgtaat cggtaaaagc tttggttccg aaatcggatt
     3661 tttgcatgga attaatttga ttaaataatt cctccaaacc aatgtcgctt ccacttgcct
     3721 gaacaaactt tccgcctccg gcttgtgcta tttcttgtag catttgcgga ttcatgcgtg
     3781 aaattacagt ttgtccagcg tcatcggttt taaatccaat tcgttcccca tcgcgcattt
     3841 caggaatagg tgaaccatcg ggagttccaa ttccaagtgt atgaacaatc actccagctt
     3901 cttttgcagc tttggcttct gctaaggcat catcctcgtg attttctcca tccgaaataa
     3961 caatgattgc tttatttcga attcctccac gaggcaaact tttcacaccc atttgaatag
     4021 ctgcacctat tgctgttcct tgatactgaa tgctttcgtt gctcaccgtg cttaaaaaca
     4081 tgcgagctgc agcgtaatcg ttggtaaatg gcaattgtag atatgcatca cccgcaaaaa
     4141 tgacgattcc aattcggtca tcgtgcaatt tatcgagtag cttgataatg gcttgtttcg
     4201 agcgttccaa acggtttgga cggatatctt cggccaacat actattcgat acgtcaagtg
     4261 caatgattaa gtcaacacct tgacgtttca cttcctcgta tttagaacca atttgagggt
     4321 tggcgaggcc gatgatgaga caaatccaag ctccaactaa taggaagaat ttagtacgaa
     4381 tctttccttg cgaacggtca ggcatgagca cttccatcaa tgctggatca ccgaaacgct
     4441 tgatggcttt acgtttccac gacatccggg cagcaaacaa aagtccgaat accggtaaaa
     4501 cggcaaggcc ccaaagaaaa aacggatagg caaatcggaa catagcttaa ggaatagaac
     4561 gcattaaagt attacgcaat agccattcgg taaacagaaa caaggcagca agcaaagcta
     4621 atggccaaaa ttcttcccgt ttacgacgaa attcagttac atcaaatcgt gtcttttcta
     4681 gcaaatcaat cttttgatag atttccttca aagcctcatt attcgtagct ctaaaatact
     4741 gtccgtcggt catcttagca attttctcta acaagggctc atcaatcctc acttccacat
     4801 aatcgaatgc aaattcacca ttcggatagc ggtaagcagg tgaataggct cttccacgag
     4861 ttccaacacc aatggtataa acacgaattc cgtaaatctg agcaatttcg gcagctgtta
     4921 aaggagaaac cgaacctgca ttattctcac catcggtaat tagaatgatc accttactcg
     4981 ccgatttgct gtctttcaaa cgcttaacgg ctgaagctaa tcccatccca attgcagttc
     5041 catccgccag gaaatcaggc tcagccacag taagtaaatt cttcaacacc gaatgatcta
     5101 ccgtaagcgg acattgtgta aacgattcac ccgaaaaaac aactaatcca atgcgatcgt
     5161 ttggacgacc atcaataaag tcgataccaa catttttagc tgcttctaag cgattaggtt
     5221 tgaaatcgcg tgcaagcata ctgggcgaaa ggtccattgc taacatgatg tcgattcctt
     5281 cggtggtcat atcctgccaa gccagtttcg tttgaggacg acacaatgcc gtaatgatta
     5341 aagcaaccgt gaagcagcgt aagatgaaag gcaaatgcat taagcgcact acagccgatg
     5401 gtttgcgccc cacgaatgga gccagtgttg ccatttgtag ctccggtttg cctttgcgac
     5461 ctttgaagat gtaccacaaa accattatgg gtacgatgag taaaaaccac aaagcccaag
     5521 gatgtgcaaa tgttatttca tcaattggag gaagacgtaa tccgttccac tcattcatgg
     5581 gtttcctcct ttcctgtttc cggttggatg atttcctgcg gacgagttaa ctccacaaaa
     5641 ttgcgagcta attccaagct tcgttcatgt tcggcaggta gcggacgttc tttggcgaac
     5701 ttcactaaat cggctagaat cagaatctgt ttcagggttt gattggtatt cgtatctgta
     5761 acaactttcc ggaacatgtg gagtgtttca tcggtagttg attccattgc aggaagctga
     5821 aattgttcct cgatgtaagc ccgaatggta tcggttatag ccgaatgata gagcttttct
     5881 cgacccgatt gccaagcttt ttcctttcca atttcgttca atgcagctaa agcacgttcc
     5941 caagctggag ttttaggtgc ttctggagct ttgggtgctt ccggacgttt acgtttcaac
     6001 cagagataaa tgcccgccac caatagcgca gcgacaagca atccaactaa aatccacgga
     6061 atatattctc caatatcgaa tggtacatct accacaccat gaatatcctt gatagcttta
     6121 gtagtatcta ccggaacagt aagtacttga aaggttagcg gatcagaaac aatgtgaacg
     6181 gtatcgcctt ttccgttgat gatatcgaat gcaaaaacgg gcatggtgtg cattccacta
     6241 tcgaaagatg tagcttcgat ttgttgcaga agttccatgc cttttcgatc ggcatcagct
     6301 accgtatcaa ccggaccacg tttgagaata tcgaaatgtc cgccaagtgt atcagttact
     6361 tctggccaaa caacacgttg tccgtcttct actattgctt taatggttaa ctgaaaaggt
     6421 tctccgagac gaatgcggta tcggtcggat ttaacgctgg cttgctgcgc ccaaaggcta
     6481 ctgcatgcga gaaggagagc aaaacaaaat gaaatcgttt ttttcatcga gttccgcggc
     6541 gtttaaagag gttacttaat ggctgaatgt aggagcgatc ggtatgcaaa actgcctgat
     6601 cgacaccagc tttactcaaa gttgacttca cgaaatcttc gtgtttttta taatgaattt
     6661 catacgattt acgcacagca cgatcggatg tatcaaccca aatggcttca cccgtttcgg
     6721 catcacgaaa gcgaacgagt cctacatcag gtaagctaat ctcacgtgga tcggtgacac
     6781 gtaatgctac tatatcgtgt tttttattgg cgattttcaa ggtgtcttcg taacctttac
     6841 cgtagaaatc ggaaagcacg aaagcggtgc agcgtttacg aatgacgttg gtcagaaact
     6901 ttaatgcttg atcgatattg gttaaacgat tctccgggcg aaaatcaatc aactcccgaa
     6961 tgatgcgtag aatatgcgat ttcccttttt taggtggaat gtatttctcg attttatcac
     7021 tgaagaaaat aaccccaatc ttatcattat tctgcatagc agagaaagct aacacagcac
     7081 acacttcggt taccagttcc attttaaact gcacctgcgt cccaaaatct ttcgaaccgc
     7141 tcacatctac caccagcata atcgtgagtt cgcgctcttc ctcatacacc ttcacatacg
     7201 ggtgatttaa tcgagccgaa acgttccagt caatcgtccg aacatcatcg cccggctggt
     7261 attctcttac ttccgaaaaa gacatacctc gacctttaaa ggcgctatgg tactgtcccg
     7321 aaaacaactg cgcagaaagt ccacggcttt tgatctcgag ttttcgtact tttttaagga
     7381 gttctttttg ttccatttgc tgatgtgctg gtgtgctggt gtgctgatgt gctagtgtgc
     7441 tgatgtgctg gtgtgctggt gaaagtctgt tatttgggat ttagtgattt ttttgatgta
     7501 cttattattt tgcttaacat tttctcaatg gacagtaact tagactcagc atctgggttt
     7561 ccaggaaggt attgtgaacg acgacataga tttaaccaat aggctgtttc tcgggcttct
     7621 tttgaggcaa ttttgagttt atgaatgaaa tctagtttac tttctgcatg ttgagcttct
     7681 gcaacgttgg ctccgattga cgttcctgac ttaaagagtt gaaatgctaa ttcgcgttga
     7741 tcttcttttt ttaatattcg actaaattga attgcttcca aagcaaattc ctctgtaagc
     7801 tgaacgatag tgtttggggc agttttcata ggatgaataa ataagagttg attaagaccg
     7861 taaaatatca aatcattatt ctcctcattt atttcatcca cctaaaacat caacatcact
     7921 gttgaaaact catctttcat cgttcaccat ttaattatca gcacatcagc acactagcac
     7981 atcagcacat caattaagga acttccacca cattcaaaat ctcactaata atattctcag
     8041 tcgtgatgtt ttcggcttcg gcttcgtagg tgagaccgat gcggtgacga agaacgtcca
     8101 tgcttacggc tcggatgtct tcagggataa cgtagccacg acgtttgatg aaggcatagg
     8161 cttttgctgc taaggctaag ctgatagatg cacgtggtga tgcaccaaag ctgatcaaag
     8221 gttgatattt cgcgaggttg tgatctttcg ggaaacgagt ggcaaatacg atgtcgacga
     8281 tgtatttctc aatcttttc
//
LOCUS       DAOOQW010000091         8281 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_10648_length_8281_cov_4.966205, whole genome shotgun sequence.
ACCESSION   DAOOQW010000091 DAOOQW010000000
VERSION     DAOOQW010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 8281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8281
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10648_length_8281_cov_4.966205"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            477..1082
                     /locus_tag="PK281_06560"
     CDS             477..1082
                     /locus_tag="PK281_06560"
                     /inference="COORDINATES: protein motif:HMM:NF014269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HOY49044.1"
                     /translation="MAVPKMLPLFPLGVVVLPGEEFYLHIFEPRYKQLIEEVRDTGIR
                     FGIPFMKNGQIQGFGTVVKLKTIFSVYPDGEMDISVQGEDIFEIQELISPFPEKLYNG
                     GISWISEYEPALKKDLLRIRPLLLEYLQLKKGEPVKLNPDRPLTSFDAAILLNLPPEQ
                     KYILIQLATEKERVIWLINELKILLQSRRMEMQLQQNYLFN"
     gene            1165..1398
                     /gene="rpmB"
                     /locus_tag="PK281_06565"
     CDS             1165..1398
                     /gene="rpmB"
                     /locus_tag="PK281_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018279505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HOY49045.1"
                     /translation="MSKVCQLTGKHAMSGNNVSFSNRKTKRRFNPNLVTKKFFIPEEN
                     AWITIRLSTSALKTIDKIGISAALKRMETKGEL"
     gene            1510..3051
                     /locus_tag="PK281_06570"
     CDS             1510..3051
                     /locus_tag="PK281_06570"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HOY49046.1"
                     /translation="MFRFILTSLLVSSCLLNAQPDLSYIHLQITPNFEKQDIQGNIDQ
                     IWNLNPSDTLIELNLYPEFIIQKVTLNKVPVTYSRFENLLSIPIKKGGSKAQISIQYA
                     GKPRQAIKPPWDGGFIWKKDINNKNWLTVACQDEGAQLWWPAPTKYSDEPDSALITAT
                     YPKDLFFKSNGKLAKDRKNKNHTRTTSWKVTYPINTYNITLNFADYIRISDTLLQANG
                     KILQLNYYPLSYNKDKAIKQFTQVKPMLRYFESYFGSYPFYRDGYSVVETPYPGMEHQ
                     SCIAYGNGYTDGYNGKDYSNIDLWFDFILIHETGHEWWGNSTSAASKRDFWLQEAFCT
                     YAEYVYVYERFGKLKAEAYMEAKKRLVSNKAPILSNDETGIDMYSKGALMLHTLQQFA
                     SSDEEWFKVLKEFALAFRSKSISTEELENWFSQKLPGVEPIFFEQYLRLANPPQLEIT
                     QENGVLSFRIRNALQNFVLPLYWIAEDGKRMRIDASANLQTLSTKGESIKPDSTSSYF
                     ELITK"
     gene            3135..5285
                     /locus_tag="PK281_06575"
     CDS             3135..5285
                     /locus_tag="PK281_06575"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HOY49047.1"
                     /translation="MRQLNYSIFVSLIAFVFISNKATAQLTVSQQTANFLVQNVLIGQ
                     GVQVSNISFTGDSAQIGQFLGGNATNLGITGGVVMSTGIVTDNVQGPNDAGGYTASPF
                     LNVSDDPDLNTIISSLGAEGVTNNICIIEFDFIPSGDSLRFKYVFGSEEYDEWVCSDY
                     FDAFGFFLSGPGIAGPYSNGSINLAQVPGTSFLVGINTVNNGSVGGSVFNPICPNGGL
                     NNSSYYLYNGLQSLQMDGFTTLLTAEAELQCGSTYHIKLVIANGGDTSFDSWVFLQAE
                     SFSSSIPNFTATNLLPDSSVIEGCSTGNLIFTRDNADEEFIIPIVYTGSAVFDVDFTG
                     LPDSIIFPIGVDSLFFTLTPVNDGITEGTELIIITYNVPNECGDTITVSDVIKIKDPY
                     ELVITTPDPTLNCPNPNYTIGAQVSGGYEPYSYSWDYNNLITPTINVPISHTDTFIVQ
                     IHDLLNCVLNQYTDTVVVTLNYDSLQTATIAQKICLGDSILLSPLVQAGLEPYTYEWV
                     NLATTDTVTVNPSDTTGYVYIITDACNISSQDTFFVNVPEYEPLTVTVNDTTICKRGT
                     VDLISFATGGDGDYTYYWSGPPVINKSTDSTSYASPETTTKYWIDVYDGCNTVATDTL
                     YVTVESCELEVGNAFSPNSDGKNDYFEIANIIYYPESTVFLYNRWGKKVLEQKAYQNN
                     WGGDDEVTAGTYFYVVDPGDGTSILKGTVTIFKD"
     gene            5341..7158
                     /locus_tag="PK281_06580"
     CDS             5341..7158
                     /locus_tag="PK281_06580"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HOY49048.1"
                     /translation="MRLSFYLLFFLFPALMLAHDTHSCAKPQEVAVRHLALKLTVDFE
                     KKIASGSAQLQLDRKQGSKELWVDTRQLNIQSITDDLGNKLKFDLQSDKPWLGKALRI
                     ELNDLSKSVTIFYATTSESAALQWLNPEQTEGKKHPFLFTQGQAILSRTWIPIQDSPG
                     IRFTWEAEITTPKELMVVMSGNNPQERSENGVYKFRMNQPVPAYLVALAVGDLQFISL
                     SERTGVYAEPSMAPRAASELADMEKMLVAAEALYGKYQWGRYDVIVLPPSFPFGGMEN
                     PRLTFATPTIIAGDRSLTSLIAHELAHSWSGNLVTNATWDDFWLNEGFTVYFERRIME
                     SLYGKDYSDMLAVLGYQDLIETLEDLKDHPEDTRLKLSLENRDADDGMTDIAYEKGCL
                     FLVSIEKKVGRDAFDAFVKSYFKEYAFKSITTEEFLKYLSRQLKLPEGVQLQTNEWIY
                     SAGIPAGTSTPKSARFEQVDAVLASWVSGNTPSVGLVVDGWTTHEWLQFIRHLPETMT
                     QEQMKELDATFGFTRSGNSEIQFAWFMAVIPNAFLQADNSLESFLKKVGRRKFVLPLY
                     TAMLKNERLSAKAKEIYQSARSGYHAVTVQTLDELFSSK"
     gene            7213..7728
                     /gene="tpx"
                     /locus_tag="PK281_06585"
     CDS             7213..7728
                     /gene="tpx"
                     /locus_tag="PK281_06585"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF001808.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HOY49049.1"
                     /translation="MSLETTSFKGKPVELLDELPGINQEAFDFTFVKTDLSESSLYDY
                     EDKVKVLIAVPSLDTGICQRETRQFNERLSAKKGVVGIVVSKDLPFAMKRFCETEGLD
                     NIVSASDYRYLDFTREYGTEMVDGPLKGLSARAVFVVDGNNKIRYVELTPDVIAEPDY
                     DRVMAEVDKLL"
     gene            complement(7793..>8281)
                     /locus_tag="PK281_06590"
     CDS             complement(7793..>8281)
                     /locus_tag="PK281_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007647137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOY49050.1"
                     /translation="DTMNFWMDIALTDLMSLLKRVDVLTINDEEARQLSGEYSLVKAS
                     KKIMEMGPKYLIIKKGEHGALLFNQDQVFFAPALPLEEVFDPTGAGDSFAGGFIGYLA
                     ETKDISFDNLKRAVIFGSAMASFSVEKFGTERIQNLSADEVDERVQQFIDLVQFDISL
                     VG"
BASE COUNT         2632 a         1680 c         1459 g         2510 t
ORIGIN      
        1 cttcgccaag gctatggctc acgaggcggg ctagttccgg ctcaaccact cacgcagaca
       61 tttcggtaca ataaatcagt ccatataaat tgatatccct aaactcaaag ttgcatcgca
      121 ctctatttca actatatata cacaaactta ccaacagatt ggttaataac tagccgttct
      181 taaacgttta tcatcgggta gctatcgcag aaaatctttt ctttcagccg gtgggtggct
      241 cgtggtcggc cccccgcggg cgtgaagact ttttgaggat tgacaggctt ttgcgaagat
      301 ttaacttttc actttccaac taaaatcatt caaaaatcac ttcttctctt aaaccacaat
      361 cttgtaacta ataaccattt atttgtaaag acttgatata gaacacactt taattttatg
      421 ctagcaaaac aagcaatctt ctttcccgtt aaattagcca tcaacctaac ctgaatatgg
      481 cagttcctaa aatgctccca ctttttcctc tgggtgtagt tgtacttcct ggagaagaat
      541 tttacctaca tattttcgaa cctcgctaca aacaacttat cgaagaagtc cgcgatacag
      601 gtatccgttt cggcattccg tttatgaaaa acggacaaat tcaaggcttc ggtacagtag
      661 tcaaactaaa aacaatcttc tccgtttacc ctgatggcga aatggacatt tccgttcaag
      721 gagaagatat tttcgaaatt caagaactta tctccccttt tcccgaaaaa ctctacaatg
      781 gcggaattag ttggatttca gaatacgaac ctgctctgaa aaaagactta ctccgtattc
      841 gaccgcttct acttgaatac ctccaattaa aaaaaggaga gccggttaag ttaaatcccg
      901 atcgaccact aactagcttc gatgctgcaa tccttctaaa tctgcctccc gaacaaaaat
      961 acattctcat tcaacttgct actgagaaag aacgagtaat ttggctcatc aacgagctaa
     1021 aaatccttct gcaatcgcga agaatggaaa tgcaacttca acaaaattat ctattcaact
     1081 aattttttcg caaaactaac ttgcgtttat ccgaaagatt ttctttcttt gcgcccctca
     1141 aataaatatc aaaccgaata ggttatgtca aaagtttgtc agttgaccgg aaagcacgcg
     1201 atgtcaggaa ataacgtttc cttctctaac cgtaaaacaa aacgccgttt caacccgaat
     1261 ctcgttacta agaaattctt cattccagaa gaaaacgctt ggattactat ccgtttatcc
     1321 acttcagcat tgaaaactat cgataaaatc ggtatttcag ctgctttgaa acgtatggaa
     1381 actaaaggcg aattataata tttattaact ttttgttctc tcttaaacgc cacctaaatc
     1441 aggtggcgtt tttgcgttta tatcacccgt aaatccgcaa aatcaagtta taacaccata
     1501 tattgcgaca tgttccggtt tatccttacc agtctactcg tatcatcatg cttgcttaat
     1561 gcacagcccg accttagtta tatccacctt caaatcacac ctaatttcga gaaacaagat
     1621 attcaaggaa atattgatca gatttggaat ttaaatcctt ccgatacgct aatcgaactt
     1681 aacctatatc ccgagtttat cattcaaaag gtaacactca acaaggttcc tgttacttat
     1741 tcccgtttcg aaaatctctt atcaattccc ataaaaaaag gtggttcaaa ggctcaaata
     1801 agcatacaat acgctggcaa acctcgacaa gccattaaac ctccttggga tggtggcttc
     1861 atctggaaaa aagacatcaa caataaaaat tggcttacag ttgcttgtca agatgaaggc
     1921 gcacaattat ggtggcccgc accaactaaa tatagtgatg agcccgatag tgcccttatc
     1981 acagcaacat atcccaaaga tttattcttt aaatcaaacg gcaaacttgc aaaagatcgc
     2041 aaaaacaaaa accacacacg cacaacttct tggaaagtca catatccaat taacacctac
     2101 aatataaccc tcaactttgc cgattatatc cgcatatcag atacacttct tcaagcaaac
     2161 ggcaaaatac ttcaactcaa ctactacccg ctttcataca acaaagacaa ggctatcaag
     2221 cagttcacgc aagtgaagcc gatgcttagg tacttcgaat catactttgg gtcttatcca
     2281 ttctatcgcg acggctattc tgtagttgaa acaccttatc ctggaatgga acatcaaagc
     2341 tgtatcgctt atggtaacgg gtatacagat ggctacaatg ggaaggatta ttctaatatc
     2401 gatttatggt tcgatttcat actcatccat gaaaccggcc atgaatggtg ggggaattcc
     2461 acatcagccg cttcaaaaag agatttctgg ctacaagaag ctttttgcac ctatgccgaa
     2521 tatgtgtatg tatatgaacg ctttggaaaa ttaaaagccg aagcctatat ggaagctaaa
     2581 aagcgattag ttagcaataa agctcctatt ctaagtaatg atgaaacagg gatagacatg
     2641 tattcgaaag gagcattaat gcttcataca ttacaacaat ttgcttcttc agatgaggaa
     2701 tggttcaaag tgctgaaaga attcgcgtta gcctttcgtt cgaaatccat ttcaactgaa
     2761 gagctagaaa actggtttag tcaaaaactc cccggagttg aacctatatt tttcgaacaa
     2821 tacctccgtt tagccaatcc tcctcaatta gaaatcactc aagaaaatgg agttctttct
     2881 tttcgaatta gaaatgccct ccaaaacttc gttctaccat tgtattggat tgcagaagat
     2941 ggaaaacgaa tgcgaataga tgcttcagcc aatttacaaa cattatctac gaagggtgag
     3001 agtattaaac ctgactcaac ttcatcttat tttgaattaa taaccaagtg atcaattagt
     3061 tcaattccga tacaggtaag cgtttcacgt tattttttta ttatttttac aaccttgcaa
     3121 aaaaattcaa tttgatgcgt caactaaact attccatttt cgtcagccta attgcctttg
     3181 tttttatttc caataaagca acggctcaat taactgttag tcaacaaaca gccaatttcc
     3241 ttgtacaaaa tgtgttgatt ggacaaggtg tgcaagtttc aaacatttct tttaccggag
     3301 attccgccca aattggtcaa ttcttgggtg gaaatgcaac aaacttgggt atcactggtg
     3361 gagttgtaat gagcacaggg attgttacag ataatgttca aggtcccaat gatgctggcg
     3421 gatacactgc ttcacccttt cttaacgtta gcgatgatcc agatttaaat accattattt
     3481 catctcttgg agctgaaggt gttactaata atatttgcat aattgagttc gatttcattc
     3541 cgtctggtga ttctcttcga tttaaatatg tatttggatc tgaagaatac gacgaatggg
     3601 tatgttctga ttatttcgac gcctttggat tctttttaag tggtccaggc atcgcaggtc
     3661 cttattctaa tggatcaatt aaccttgctc aggttcccgg aacttcattt cttgttggta
     3721 ttaatacagt taataatgga agtgttgggg gtagcgtttt taatccaatt tgcccaaatg
     3781 gagggttaaa caacagctct tattacttgt acaatggttt acaatcgctt caaatggatg
     3841 gctttacaac gctattaact gctgaagctg aacttcaatg tggatctact tatcacataa
     3901 aacttgttat cgcaaatggt ggagatacat ccttcgattc ttgggtattc cttcaagccg
     3961 aaagttttag ctcaagcatt cctaatttca ctgcaactaa cttactacct gatagttcag
     4021 taattgaagg atgtagtaca ggaaacttaa tctttacccg agacaatgct gatgaggagt
     4081 tcattattcc aattgtatat acaggttctg cggtctttga tgtagatttt acgggtctac
     4141 ctgattcaat tattttccca attggtgttg attcattgtt ttttacctta actcctgtta
     4201 atgatggaat tactgaagga acagaattaa tcatcattac ttataatgtt ccaaatgaat
     4261 gtggtgatac aattaccgtt tcagatgtta ttaaaattaa agatccatat gagttggtaa
     4321 tcacaactcc agatccaaca ctaaactgtc caaatccaaa ctacactatt ggcgcacaag
     4381 tttcaggagg atatgagcct tacagctatt cttgggatta caacaacctc attactccta
     4441 cgataaatgt tcctatatca cataccgaca cattcatcgt tcaaattcat gatcttctca
     4501 attgcgttct caatcaatat actgatactg tagtggtaac gcttaattac gattcattac
     4561 aaacagcaac gatcgctcaa aaaatttgtc ttggagattc tattcttcta agtcctctag
     4621 ttcaagcagg attggaacca tatacatatg aatgggtgaa tctcgcaaca accgacacag
     4681 taacagttaa tccttcagac acaactggat atgtttatat cattaccgat gcttgtaaca
     4741 tttcgtctca ggatactttt tttgtaaatg ttcctgaata cgaaccactt accgtaacag
     4801 ttaatgacac aacaatttgt aaaagaggaa cagttgatct tatttctttt gccacaggtg
     4861 gtgatggaga ttatacctac tattggtctg gtcctcctgt gataaacaag agtaccgatt
     4921 ctactagtta cgcgtcaccc gaaacgacca ccaaatattg gattgacgta tacgatggtt
     4981 gtaataccgt tgcaaccgat acattgtatg ttacagtaga aagttgtgaa ttagaagttg
     5041 gaaatgcatt ctcacctaat tcagatggga aaaatgatta cttcgaaatt gcaaacatta
     5101 tctactatcc tgaaagcaca gtcttccttt acaatcgctg ggggaaaaaa gtattagaac
     5161 aaaaagctta tcaaaataat tggggtggcg atgacgaagt aactgcaggt acttacttct
     5221 atgtggtaga tccaggtgat ggaacaagta ttctcaaagg tactgtcact attttcaaag
     5281 attgatttaa ctaaacttgt tgaacaggcg tccttgtggc gcctgttctg ttttcaccct
     5341 atgcgtttat ccttttatct cctcttcttt ctctttcctg cacttatgct agctcatgac
     5401 acacacagct gcgcaaaacc tcaagaggtt gcagtgagac acttagcttt aaaactaact
     5461 gtcgatttcg aaaagaaaat agcatccgga tcggctcaac ttcaactcga tcgtaaacaa
     5521 ggatcaaaag aattatgggt agatactcgt caactcaaca tacagtcaat aaccgatgac
     5581 ctagggaata aactcaaatt cgatttacaa tctgacaagc cttggctagg aaaagcgctc
     5641 cggatagaat taaacgattt atctaagtcg gttaccatct tctatgctac cacttctgaa
     5701 tctgctgcac ttcaatggct taatccagaa cagactgaag gtaaaaagca tcctttttta
     5761 tttactcaag gacaagcaat actcagtaga acatggattc caattcaaga tagtccaggt
     5821 attcgattta cttgggaggc agaaataact actccaaagg aattaatggt tgttatgagt
     5881 ggcaataatc cgcaggaaag aagcgaaaat ggtgtttaca agtttcgcat gaatcaacct
     5941 gtacctgcct acttagtagc tttagctgtt ggagatcttc aattcatttc tttgagtgag
     6001 cgtactggtg tatatgccga accaagtatg gctccacgtg cagcctctga attagccgat
     6061 atggaaaaaa tgttggttgc agctgaagct ctttatggga aatatcaatg ggggcgatat
     6121 gatgtgattg ttcttcctcc atcattccct tttggtggta tggaaaaccc tcggttaacc
     6181 tttgctacac ctacaatcat tgcaggagat agatcactta catctcttat cgcacatgaa
     6241 ctcgcccaca gttggtcagg taatttagtt acaaatgcaa cttgggatga tttctggcta
     6301 aacgaaggtt tcactgtata ctttgaacgc cgcattatgg aatctttata tggtaaagac
     6361 tattccgata tgctagcagt attaggttat caagatctta tcgaaacact tgaagattta
     6421 aaagatcatc cagaagatac acgtctgaaa ttaagtttag aaaaccgaga tgcagatgat
     6481 ggaatgacag atatagctta tgagaaaggc tgcttatttc tagtttctat tgagaaaaaa
     6541 gttggaagag atgcttttga tgcttttgta aaatcttatt tcaaagaata cgctttcaaa
     6601 tccattacaa ccgaagagtt tcttaagtat ttatctcgtc aattaaaact accagaaggt
     6661 gtacaacttc aaactaatga gtggatatac tcagctggta ttccagcagg aacttctact
     6721 cctaaatcag ctcgatttga acaagttgat gctgttttag catcatgggt ttctggaaat
     6781 actccttctg taggtttagt tgttgatgga tggactacac atgaatggct tcaattcata
     6841 agacatttac ctgaaacaat gacccaagaa caaatgaaag aactggatgc cacttttgga
     6901 tttacccgtt caggtaattc tgaaattcag tttgcgtggt tcatggctgt aataccgaat
     6961 gcattcttac aagcagataa ttctcttgaa tcgtttttga aaaaagtagg acgaaggaaa
     7021 tttgttttac cactttacac tgcgatgctt aaaaatgaac gtctttcagc taaagcaaag
     7081 gaaatatacc aatcagctcg atcaggatac catgcagtta ccgttcaaac actggatgaa
     7141 ttattttctt caaaataata tcgagtaacc aatacactca aaaccttaga tttgtcaaac
     7201 agaataataa ctatgagctt agaaacaact tctttcaaag gaaaaccagt agaacttctt
     7261 gacgaacttc cgggaatcaa tcaggaagca tttgatttca ctttcgttaa aaccgattta
     7321 agtgaaagta gcctgtatga ttatgaagat aaagtgaagg tgctaatcgc agtaccaagc
     7381 ttagatacag gaatttgcca aagagaaaca cgtcaattta acgaacgcct atcagctaaa
     7441 aaaggcgtgg taggaattgt cgtttccaaa gatcttcctt ttgcaatgaa acgtttttgc
     7501 gaaacagaag gattagataa cattgttagt gcatccgact atcgttacct cgatttcaca
     7561 cgtgaatatg gtactgaaat ggttgatggt ccattaaaag gactaagcgc tcgtgctgta
     7621 tttgtcgtag acggaaataa taaaatccga tatgtagaat taacaccgga tgttattgca
     7681 gagccagatt atgacagagt aatggctgag gtcgataaac tgctttaagt gaattcaata
     7741 aataaaaaaa ggcgccccaa aagcgccttt ttttattgct gtaagttata aatcaaccta
     7801 ccaatgaaat atcaaattgt acaaggtcga tgaattgttg aactcgttca tctacttcat
     7861 cagcacttaa attttggata cgttctgttc caaatttctc cacactaaaa gatgccattg
     7921 ccgagccaaa aattacggca cgttttaaat tatcaaacga gatatctttt gtttctgcca
     7981 agtatccaat gaaacctcct gcaaagctat ctccagctcc cgttggatca aaaacttctt
     8041 ctaaaggtaa tgctggtgca aaaaacactt ggtcttgatt aaataacaag gctccgtgtt
     8101 cacctttctt aattatcagg tatttaggac ccatttccat aatcttcttg gatgctttaa
     8161 ctaaagagta ttcacccgaa agttgacgag cttcttcatc attgatggtc aatacatcca
     8221 ctcgctttaa taatgacatc aaatcagtta aagcgatatc catccagaaa ttcatggtat
     8281 c
//
LOCUS       DAOOQW010000092         8257 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_10689_length_8257_cov_5.091075, whole genome shotgun sequence.
ACCESSION   DAOOQW010000092 DAOOQW010000000
VERSION     DAOOQW010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 8257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8257
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10689_length_8257_cov_5.091075"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="PK281_06595"
     CDS             complement(<1..444)
                     /locus_tag="PK281_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009642454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOY49051.1"
                     /translation="MNQILKGKRGIIFGALDEPSIAWKVAEQAHAEGASFVLTNAPIA
                     MRMGEINKLAEKCNAQIVPADATSDEDLNNLFTKAQEILGGKIDFVLHSIGMSPNVRK
                     ARPYTDLNYEFFHKTIDVSAMSLHRVLQAAHKQDALNEWGSVVALT"
     gene            complement(534..2195)
                     /gene="recN"
                     /locus_tag="PK281_06600"
     CDS             complement(534..2195)
                     /gene="recN"
                     /locus_tag="PK281_06600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HOY49052.1"
                     /translation="MLQKLSIRNYALIQELSIDLRPGLTIITGETGAGKSILLGALSL
                     LLGDRADPSVLRDPNQKCIVEATLAIQKYNLSDFFRAHDLDEETNCIIRREINPAGKS
                     RAFINDTPVNLNQLRELGARLIDIHSQHDTLLLNSSAFQLQLLDAPAGTVELLLQYRD
                     LFSNWKKKKKELEDLRAEESRIRAEQDFLQFQFDELETVRIRPNESQLLEEEFEVLSH
                     AEDIKVHLSSAIQGLDDENRGVIPALRQVQNSIQQAAKYSPAIATIQERIQSSLIEIR
                     DILAESEHQAEHLDANPIRLEELRSRLDQLNHLLNKHRVSTSDELLVLQQQFEERLNQ
                     AAGLNEDLVRLQQELDSLYTQVIDKAERISEKRKAIIPRIVKESTAVLARLGMPKTRL
                     QIELSKREYPETNGLDKVNVLFSANAGSIPQELSKVASGGELSRLMLSLKKIIAGSVA
                     LPTIVFDEIDTGISGSVADSMGEILKEMGTELQVIAITHLPQIASKGDDHLKVIKSTT
                     GKETSSLLIRLNEADRIDEIAGMLSGKELSEAAISNARSLLGMGT"
     gene            2300..2938
                     /locus_tag="PK281_06605"
     CDS             2300..2938
                     /locus_tag="PK281_06605"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOY49053.1"
                     /translation="MQGLKELIAKGESQTLEFKFELNSARKIAATISAFSNAQGGKIL
                     VGVKDNGALAGIRLDEEVYVLDAAASMYCDPPVTLKTRRWEVDGKFILEAEVEESIHK
                     PVKAQTEPEEWKAWLRVGASNRLANPVHLALWQIRNPGKEVPAQFTEKEQKVLQVLQD
                     KKWVSLNQACKWSKLPRFLVAKTLAHFMQWGIVYTELEESGGFVFMLREDFP"
     gene            2953..3090
                     /locus_tag="PK281_06610"
     CDS             2953..3090
                     /locus_tag="PK281_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49054.1"
                     /translation="MTIWKITLIGLALLSIPWLVLKLVLYVCKKDQQQHEAQSENDSI
                     H"
     gene            complement(3087..4634)
                     /locus_tag="PK281_06615"
     CDS             complement(3087..4634)
                     /locus_tag="PK281_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49055.1"
                     /translation="MKRCVFRILILWIFISPLAAQKSIQWGSDIFKPSSSTAFQVIGN
                     WPDGIIMQARTRTKLFSPGKTFIQRYDNLTLLPQFNKEIKLETTKGSKTLEYAVLERV
                     GDKPVLMATYFNSEKDKIELYGRLYDIEGEPSEKEKKIAEFPATRKSQLQALNFVQSS
                     DSSSMLCFFSEKFDKYSNEKMEFNLFNPQLEIIWNRSIEFPYKGSNLQIHRTVVDPQG
                     RVYMLVRVIFDKDELANKGGPRYRYSLVTFASDTTLVEDYEISLGDQYISDIDLILGK
                     DNQIICSGFYSERGQGQAAGTFFLEIDRNSKAVLDKKLIPFDKGFAASFMETSRIRGK
                     MELSDFKLDHFVRFKDGTFGLVAEQYLEDEICYQDFRTGMYSCNYYYYYNNIIVVKMD
                     SIGDVIWTADIPKYQQSSNDGGFYSSYVFGFDGESMHFVFNDHPKNLTETDQSRAHIM
                     NNVRRSVPVYARISNDGQFSRRMVSDEKKSRLFMVPQYSTQISDRSIWLVGITTNKYR
                     TGVLSFD"
     gene            4805..>6048
                     /locus_tag="PK281_06620"
     CDS             4805..>6048
                     /locus_tag="PK281_06620"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HOY49056.1"
                     /translation="MKRFLFPALLIIGAISLLWSTIQPTSPQVDSIKPPFEGTNKAWV
                     DSVMKVLTPDQRIGQLFMVAAYSNKPKSHQDEITKLIKDYQIGGLIFMQGGPVRQAKL
                     CNYYQQISNVPLMISIDGEWGLAMRLDSTEAFPKQMTLGAIQNDSLIYFMGKEIARQC
                     RRMGIQVNFAPVVDVNNNASNPVINMRSFGENKYQVATKGIAYMKGMQDALVLSNAKH
                     FPGHGDTDSDSHKTLPHINQPRARLDSLELYPFKELIRNGLGSVMVAHLFVPAFDSTK
                     GRPTTLSPLVVTDLLQKELGFSGLIFTDALNMKGIAASNQPGFADMRALLAGNDVLLF
                     AEDVHTGIQQIKKAIEEKKITQEEIDRRCRKILSAKAWLGLKKSPRIPLANLNQNLHT
                     SSSELLRRKLTEASLTVLENKS"
     assembly_gap    6049..6148
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6149..7615
                     /locus_tag="PK281_06625"
     CDS             <6149..7615
                     /locus_tag="PK281_06625"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOY49057.1"
                     /translation="DLIAEIKKNSRVVVNLFANPYSLSSLDSKSTPDGLIVSYEDNPL
                     SQEISAQLIFGGIQANGRLPVTVSTAYPAGKGIETSAPIRMKYTIPDEVGIRPENLRA
                     VDSIAKLAIRMKATPGCQVLIAKEGKIFYNKSFGTHTYESSSSPVKNSDLYDIASITK
                     VAASTMAIMRLTDEKFIDIDKTVGDYIPFLNVTNKGSLRLRDILAHQAGLPAWIPFYT
                     RTISNETMRKKYYRKELSDSFRLKVAEGLYIRNDYPDSMLAQIAACEVNKNKEYKYSD
                     LGYYFFKYIIEHHTGKSLDSYVDSVFYKPMGLSRITYNPLNKFKKEEIIPTEQDNLFR
                     KQLVHGYVHDQGAAMMGGVGGHAGIFADAGDLAALMQMILNTGEYGGKRYLTKATIDE
                     FVKCQFCENENRRGLGFDKPEMNYGKTGPTCKCVSALSFGHTGFTGTIAWVDPEQKLI
                     YVFLSNRVYPDAENKKINTLATRVEIQEAIYQAIDKGY"
     gene            complement(7668..8069)
                     /locus_tag="PK281_06630"
     CDS             complement(7668..8069)
                     /locus_tag="PK281_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009086950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49058.1"
                     /translation="MKRIFVFATALLFSVAVSAQTPASKPTTKPKANSGQVKSSTPTK
                     QAPPKQVAPTKETKTQQSTPKIAPSNKVAPSTTKDKVKKDGTPDKRFKENKHLKKDGT
                     PDKRYKENKSDEKRKAATPAPVRSNVPANKK"
BASE COUNT         2484 a         1514 c         1620 g         2539 t
ORIGIN      
        1 ggttagcgca accacagatc cccattcgtt taatgcatct tgtttatgag cagcttgtaa
       61 tacacggtgt aacgacatag ccgaaacatc tattgtctta tgaaagaact cataattcaa
      121 atcggtatac ggacgagctt tacgaacgtt cggtgacata cctatagaat gtagaacgaa
      181 atcgatttta ccacctaaaa tttcctgtgc tttggtaaac aggttattta aatcttcatc
      241 agaagttgca tcggcaggaa caatttgagc attgcatttc tctgctaatt tgttgatttc
      301 gcccatacgc atggctattg gagcgttcgt caatacaaaa gaagctcctt cggcatgagc
      361 ttgttcagca actttccaag caatacttgg ctcatctaat gctccgaaga ttattccgcg
      421 ttttcctttt aaaatctgat tcatgggtat tgtatatgtg ttgaattcgt tgataaataa
      481 gtgcttacgt taaaacaccg attgttttgc gagtgtaaat gtaaaagttt tgtttaagtg
      541 cccatgccaa gtaatgaacg agcattggaa atagctgctt ctgacaattc ttttccgctt
      601 agcataccgg caatttcatc aatacgatca gcttcgttta agcgaatgag taaggaactt
      661 gtttctttac cagttgttga tttgataact ttcaaatgat catcgccctt actagcaatt
      721 tgaggtaaat gggtgatggc aataacctgt agttcggttc ccatttcttt gagaatttcg
      781 cccatgctat cagctactga acccgaaata cctgtatcaa tttcatcaaa aacgatggtt
      841 ggtaatgcta ctgatccagc aattatcttc tttaaactca acattaaacg ggaaagttct
      901 cccccactgg ctactttact caactcttgc ggaatagaac cagcatttgc cgagaaaagc
      961 acattcactt tatctaaacc attcgtttcg ggatattcac gtttagataa ctctatttgt
     1021 aaccgcgttt ttggcatgcc caaacgtgct aatacagctg tgctttcctt cactattcga
     1081 ggaatgattg ctttccgttt ctcagaaata cgttcagctt tatcaattac ttgagtgtaa
     1141 agtgaatcga gttcttgctg caaacgaact aaatcttcat tcaaacccgc agcttgattg
     1201 agtcgttctt caaactgttg ttgaagtacg agtaattcgt cgctggttga aactcgatgt
     1261 ttattcaata gatgatttaa ctgatcgagt cggctgcgta attcttctaa acggattgga
     1321 ttggcatcta aatgttcggc ttgatgttcc gactcagcaa gaatatctcg tatttctatt
     1381 aatgaagatt gaatacgttc ttgaatagtt gcgatagcag gagaatattt agctgcttgc
     1441 tgaattgaat tctgcacttg gcgcaaagcc ggaatgactc cacgattttc atcatctaat
     1501 ccttgaatag ctgaagaaag atggacttta atgtcttcgg catgacttaa tacttcaaac
     1561 tcctcctcga gcaattgcga ttcattggga cgaattcgaa cggtttctaa ttcatcgaac
     1621 tgaaactgaa ggaaatcctg ttcagctcga atgcgacttt cttcggcacg taaatcttcc
     1681 agttcttttt tcttcttctt ccaattagaa aataaatccc ggtattgtag caataattcg
     1741 acggtgccgg cgggagcatc gagcaattga agctgaaatg ctgaactatt caacagtaat
     1801 gtatcgtgct gagaatgaat atcaatgagt cgagcaccta attcacgaag ctgattgagg
     1861 ttgacgggcg tgtcgtttat aaatgctcga gactttccgg ctggatttat ttcgcgacga
     1921 atgatacagt tagtttcttc atccaaatcg tgggcacgaa aaaagtcact cagattatac
     1981 ttctgaatag ctagcgtagc ttcaacaatg catttttggt ttgggtcgcg taagacagaa
     2041 ggatcggctc ggtcgcccaa taaaagtgat aatgctccta ataggattga ctttcctgca
     2101 cctgtttcac ctgtaataat ggttaatcca ggacgcaagt cgatgctaag ttcctgaata
     2161 agcgcataat ttcggataga gagcttttga agcatagtgc aaatttaacc cagtcgacaa
     2221 actatcctga gttaattaaa gtgaattgct cacatcccta ttaacaagac tgttcatatc
     2281 acttgattat attcgcccca tgcaagggtt aaaagaatta atagctaaag gcgaaagtca
     2341 aacacttgaa tttaagtttg agcttaattc tgcccgaaaa attgcagcaa ccatatcggc
     2401 tttttcaaat gctcagggag gaaaaatatt agttggtgtg aaggataatg gcgcacttgc
     2461 gggaattcga ttggatgaag aagtatatgt gttagatgct gcggcttcga tgtattgtga
     2521 tccgccagtc acgctaaaaa cacgacgttg ggaagtggat gggaaattta tcctagaagc
     2581 cgaagtagaa gaatctattc ataaaccggt aaaagctcaa accgaacccg aagaatggaa
     2641 agcttggctt cgtgtgggag catcaaaccg tttggctaat ccggttcatt tagctttgtg
     2701 gcagattcga aatcccggaa aagaagtccc agcacaattc accgaaaaag agcaaaaagt
     2761 gcttcaggtt ttacaagaca aaaaatgggt cagcttaaat caagcctgta aatggagcaa
     2821 attgccccgg tttttagtgg cgaaaacgct agctcatttt atgcaatggg gaatcgttta
     2881 tacggaatta gaggaaagtg gtggctttgt ttttatgttg cgggaagatt ttccttaacc
     2941 gttatttgcc gcatgacgat ttggaaaatt acgctgattg gacttgcctt actttcgatt
     3001 ccttggctgg tgctaaaact tgtactttat gtatgcaaga aagatcagca gcaacacgaa
     3061 gctcaatcgg aaaatgattc cattcattaa tcaaacgaca aaactcccgt tcgatattta
     3121 ttcgtggtaa ttcccaccaa ccaaatggaa cgatcactta tttgagtgga atattgcggc
     3181 accatgaata aacgggattt cttttcgtct gacaccatac gacgactgaa ttgtccgtca
     3241 ttactaatac gagcataaac aggtacggat cttcgcacat tgttcatgat atgcgcacga
     3301 gattgatctg tttctgttaa gttttttggg tgatcgttaa aaacgaaatg catgctttct
     3361 ccatcaaaac caaacacata agaagaataa aatccaccat cgttggagct ttgctgatac
     3421 ttaggaatat cggctgtcca aataacatct ccaatcgaat ccatcttcac aacgattatg
     3481 ttattgtagt aataatagta attacagcta tacatacctg tccgaaaatc ctgataacaa
     3541 atctcgtctt cgaggtattg ttcagcaact aaaccgaatg tgccatcttt aaatcgaaca
     3601 aaatgatcaa gtttaaaatc gcttaattcc attttacctc gaatgcgact cgtttccata
     3661 aaactagctg caaatccttt atcgaaagga atgagttttt tgtcgagtac ggctttagaa
     3721 tttcgatcta tttctaagaa aaatgttccg gctgcttgtc cttgcccgcg ttcggaataa
     3781 aaaccactgc aaataatctg attgtctttt cctaggatta ggtcgatatc cgaaatatat
     3841 tggtcaccta aactaatttc gtaatcctct accagtgtag tatcgctggc aaaagtcact
     3901 aagctatacc gatatcgggg accgccttta ttagctaatt cgtctttatc gaaaatgaca
     3961 cgtacgagca tatatacgcg accttgcgga tcaacgactg ttcgatgtat ttgaagatta
     4021 ctgcctttat atggaaattc aatactccga ttccagatga tttctaattg tggattgaat
     4081 aaattaaatt ccattttctc attcgaatat ttatcgaatt tttctgagaa aaaacaaagc
     4141 atggaactgc tatcgcttga ttgaacgaaa ttcagtgctt gcaactgact tttacgcgta
     4201 gcagggaatt cagcaatttt cttttctttt tcacttggtt caccttcgat atcgtaaaga
     4261 cgaccgtata attctatttt atctttttcg gaattaaaat aggtagccat caaaacaggc
     4321 ttatctccta cccgctccaa tacagcatat tcgagcgttt tgctgccttt agttgtttct
     4381 aatttgattt ctttattgaa ttgtggtaaa agagtcaaat tatcatatcg ttggataaat
     4441 gtttttccgg gtgaaaataa ttttgttcga gttcgggctt gcataattat cccatcgggc
     4501 caatttccta tgacttgaaa cgcggttgaa ctggaaggtt tgaaaatatc agaaccccat
     4561 tgaatactct tttgagcagc aagtggtgaa atgaaaatcc acagaattag tattcgaaaa
     4621 acacatcttt tcattgggct caatttacga aaaggcacat agcttcgatg ataaaacgta
     4681 gaatatctag ttgcattatt atagaattaa acatttcacg aatacatgtt gatacttatt
     4741 ccattttctt gggaaactcg catacggcgc aggctacatt tacgttttga tacgaattgc
     4801 aaacatgaaa cgcttccttt ttcctgcatt attaattatt ggagcgatat cgctactctg
     4861 gtctaccatt cagccaactt cacctcaagt tgattcgata aaacctcctt tcgaaggaac
     4921 gaataaagct tgggtcgata gtgtgatgaa ggtacttacg ccagatcagc gtatagggca
     4981 gttgtttatg gtggcagctt attcgaataa acctaaatcg catcaagatg aaataactaa
     5041 actgattaaa gattaccaga ttggtggttt aattttcatg cagggtggac cggttcgaca
     5101 agctaaattg tgcaattatt accaacagat ttcgaatgtt cctttgatga tttcgattga
     5161 tggagaatgg ggattagcca tgcgtttgga ctcaaccgaa gcatttccta aacaaatgac
     5221 tttaggagca attcaaaacg attctttgat ttattttatg ggaaaagaaa ttgcccgaca
     5281 atgccgccga atgggtattc aggtgaattt cgcaccggta gttgatgtaa ataataatgc
     5341 atcaaatcca gtaataaata tgcgttcgtt tggtgagaat aaatatcaag ttgcaaccaa
     5401 aggaattgcc tatatgaaag gaatgcaaga cgctttggta ttatcaaatg cgaaacattt
     5461 ccctgggcat ggagataccg attccgattc tcataaaaca ctaccgcata taaatcaacc
     5521 acgtgctcga ttagattcgt tggaattata tccatttaaa gaactaatcc ggaatggttt
     5581 aggcagcgtt atggttgctc atttatttgt cccagccttt gattcaacaa aaggtcgacc
     5641 tactacccta tcaccattgg ttgttaccga tttattgcaa aaagaattag gatttagtgg
     5701 attaattttc acggatgcac tcaacatgaa aggtattgcc gctagtaatc agcctggttt
     5761 tgctgatatg agagctttac ttgcaggaaa tgatgtgtta ttatttgccg aggatgtaca
     5821 tacaggtatc caacaaataa aaaaggcaat tgaagagaaa aaaataacgc aagaggaaat
     5881 tgatcgccgt tgccgtaaaa tactcagtgc aaaagcttgg ttgggattga aaaaatcgcc
     5941 acgtattcct ttagcaaatc tcaaccagaa tttacatacc agtagttctg aattattgcg
     6001 tagaaaactg actgaagctt ctttgactgt tttagaaaac aaatccatnn nnnnnnnnnn
     6061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnga tttaattgct gaaataaaaa agaattcacg
     6181 tgtagttgta aacttatttg ctaatccgta tagcttatca agtctagact cgaagtcaac
     6241 tccagatggt ttaattgttt cgtatgaaga taatccgcta tcacaagaaa tatccgccca
     6301 attaattttt ggaggaattc aagcaaatgg aagattaccc gttactgtaa gtacagccta
     6361 tccggcagga aaaggaattg aaacatctgc gcctattcgg atgaaatata cgattcccga
     6421 tgaagtagga attcgtccgg aaaatttgcg tgctgttgat tctatagcaa aactggcaat
     6481 tcgaatgaaa gctactccag gttgtcaggt tttaattgct aaagaaggaa agatatttta
     6541 taataaatca ttcgggacgc atacttacga atcgagttct tcacccgtta agaatagcga
     6601 tttatatgat atagcttcaa ttacaaaagt tgcagccagc actatggcaa tcatgcgttt
     6661 aacagatgaa aagtttatcg atattgataa aacagtagga gattacattc cctttttaaa
     6721 tgttaccaat aagggaagtt tacgattacg tgatatcctt gcacaccaag ctggtttgcc
     6781 tgcatggatt cccttttata cacgcaccat ttcaaatgaa acaatgcgga aaaaatatta
     6841 ccgcaaagaa ttgagcgata gtttccgtct caaagtagca gaaggattat acatacgcaa
     6901 tgattatcca gattcgatgt tggctcagat tgcggcttgt gaagtcaata aaaacaagga
     6961 atataaatac agtgatttag gatattattt tttcaaatac atcattgaac atcatactgg
     7021 aaaatcgctt gattcatatg tagatagtgt gttttacaaa ccaatgggtt tatcgagaat
     7081 tacctataat ccattgaata aattcaaaaa ggaagagata attccaaccg aacaggataa
     7141 tttatttcgc aagcaattag ttcatggtta tgtgcacgat caaggagcag caatgatggg
     7201 aggagttggt ggacatgcag gtatatttgc agatgcaggt gatttagcag cattgatgca
     7261 aatgatttta aacacgggag aatatggagg gaaacgttat ttgacgaaag ccactataga
     7321 tgaatttgtc aaatgtcaat tctgtgaaaa cgaaaaccga cgtggtttag gatttgacaa
     7381 accagaaatg aattatggta aaacgggtcc aacttgcaaa tgtgtgtcag ccttgagttt
     7441 tggacatact ggatttacgg gaacaattgc ttgggttgat ccagagcaga aattgatcta
     7501 tgtcttttta tccaaccgcg tttatcccga tgccgagaat aagaagataa atacacttgc
     7561 tactcgagta gaaattcaag aagccattta tcaagcaatc gacaaaggct attaaataga
     7621 aaagccctaa agaaattctt tagggctttt aatttgagaa ttaattatta cttcttgttt
     7681 gctggaacat tagaacgaac tggagctggt gtagctgctt ttcgcttttc gtctgattta
     7741 ttttctttat aacgtttatc aggagttccg tcttttttca ggtgtttgtt ttctttaaat
     7801 cgtttatcgg gagttccgtc ttttttaacc ttatccttgg ttgttgatgg agctacttta
     7861 ttgcttggag caattttcgg agtcgattgt tgagtttttg tttctttcgt tggagctact
     7921 tgtttaggag gagcttgctt tgttggtgta cttgacttca cctgacctga attagccttc
     7981 ggtttagtag tcggtttact tgcaggagtt tgagccgaaa cagctacact aaagagtaaa
     8041 gccgtggcaa atacaaatat tcgtttcatg tgggttgaat ttgttttatc gtttacaagg
     8101 attgtgcctc tatggtttaa cgtgtctctt cttcattcct tccaaggcgg atagataacg
     8161 ttaagaaaaa ctgaatagta cttaacttct gctcatcaac caatccaggc aatcgactcc
     8221 catgaagtgc ataggaaaag tcttccagat tgacttt
//
LOCUS       DAOOQW010000093         8239 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_10723_length_8239_cov_4.937805, whole genome shotgun sequence.
ACCESSION   DAOOQW010000093 DAOOQW010000000
VERSION     DAOOQW010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 8239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8239
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10723_length_8239_cov_4.937805"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="PK281_06635"
     CDS             complement(<1..336)
                     /locus_tag="PK281_06635"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOY49059.1"
                     /translation="MRKILLLSFVFLCYIGKPVIAQDTRFQLADSLFNLVQKKNRAMG
                     SVCIRKNGKIEYQRSFGWAEALAHVRSNDSTAYRIGSISKVFTSVIIHQLIEEKKLKL
                     DQKLGEFFPD"
     gene            complement(367..1566)
                     /locus_tag="PK281_06640"
     CDS             complement(367..1566)
                     /locus_tag="PK281_06640"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HOY49060.1"
                     /translation="MSKLNSIQEAIEDIRQGKIIIVVDDENRENEGDFLTAARNATPE
                     VINFMATQGRGLICAALTEERCEELKLEMMVSQNTSSHDTAFTVSVDLLGHGCTTGIS
                     ASDRSKTMKALIDPSIRPEDFARPGHIFPLKAKTGGVLRRAGHTEATIDLSRLAGFEP
                     AGVLVEIMNEDGSMARLPELLQLAEKLDMKIISIEDLIKYRLEHDSLIRREVEVDLPT
                     EHGDFKMVAYTQTNTSEQHLALVKGEFLPGEPVLVRVHSSCMTGDIFGSCRCECGPQL
                     QAAMQQIQLEGKGVIVYMNQEGRGIGLLNKLKAYKLQEEGYDTVEANIKLGFKMDERD
                     YGVGAQILRDLGVSKLRLLTNNPTKRAGLIGYGLEIVENVPIVIEANIHNARYLATKR
                     DKMGHSF"
     gene            complement(1595..2782)
                     /locus_tag="PK281_06645"
     CDS             complement(1595..2782)
                     /locus_tag="PK281_06645"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOY49061.1"
                     /translation="MKILVALSRFPFPTDKGDKLRAWHQLLGLASEHEVHVFCTSDES
                     VTDESLEKAKSVFASVRVFQLKRIPVLARLAKNFFQSKPYQVAYFSDADAIRQFQEWY
                     QQLKPVVVYCQLARMAEFVKSLQGCRKILDYQDAFSIGLQRRMNHDAWWKRMMISSES
                     SRLSRYETEIFDLFDIRTMISSQDALLINHPQRAEIRIIPNGVDTDFYKPEPRARTLD
                     LLFTGNMQYEPNVNCVVYMVNQVLPLLNQEFPHIQFVAAGKNPSRELKQLNSRHLQLT
                     GWVNDLRSYYQQTRLFVAPMQIGIGMQNKILEAMSMGLPVITSTLANNAIGARHGKEV
                     WIADTPEKVAEAIAFLLNNTELALTIGQNAREFMVAEFSWNKQNNRLNRLITGSTVKD
                     RIL"
     gene            complement(2779..3969)
                     /locus_tag="PK281_06650"
     CDS             complement(2779..3969)
                     /locus_tag="PK281_06650"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOY49062.1"
                     /translation="MNVLQLCTKPLFAPFDGGKIAMFSIANGLHQAKHTVTQWMISTP
                     THPFPASIPKDYPFTIQHHFTDTRVKPWDAFINLFQGNSYNLSRFNDAAAIQQQVAWL
                     KQNPVDIIQAESIYALTGIADLRKASKAPIILRAHNVEFLIWERMANQAQNPLKKAYL
                     HMLSRRLKKEELDLCNACDGIVVMSDVDAEVFRKLGVHVPIEVIGIATDLSPLTSQTG
                     LEKKQLFHLGAMDWLPNREGVEWFIHQVWPSLHAALPDWKLVLAGKNMPSEYEEMQAA
                     GIEVKQAPDAAEFMQSEGIMIVPLLSGSGIRVKIIEGLALGRIILTTPTGLEGIPAED
                     GKHLFVAHKPADFLTHLSQLLTSPNLAKRISENALTFANEHFRSEVLTQRLLQFYEKI
                     STRV"
     gene            complement(3966..5636)
                     /locus_tag="PK281_06655"
     CDS             complement(3966..5636)
                     /locus_tag="PK281_06655"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOY49063.1"
                     /translation="MKRIDSFVFKSYIGPFILTFFIALFILLMQFVWKYVDDLVGKGL
                     EWFIIFKLLGLVAITMISLALPLAVLLSSLMTFGNLGEYMELTAMKSSGISLQRAMRS
                     VLIFALFLSISAFYFSNNILPLANLKMKTLLQDISNKRPELNIKPGIFNSDIQNYVIR
                     IGKKSNDGKKLEQIMIYDHSNRMGNDRVIIAERGNMGLSVDKRYLELTLVNGHTYTEA
                     EPGRKQKGRYPFLRENFQEDLIRFDLSIFELTKTDENVYKRNYQMLNITQLMLSADTL
                     KEALAQEKHFLPGVLLHGPFFDTTRSYSTVWTDPKLVKRNRSKADTTKAQTTTSKDTL
                     HDSTTHRIALRTETARLMARGRIDSVQILNERGIQKPAFLSDEPRILTNNVPLKSKTQ
                     VLDNFSKNDKIKILDGAMNNLRSNKSTIDYSMQNFEQDQERINRHEIEWHRKFTLSFA
                     CLILFLIGAPLGAIVRKGGLGMPVIFSLIFFVLYHMISITGEKLAREGVLTAFWGMWL
                     SSAILLPIGIFLSYKATTDSALFDADAYKRFWNRFVMKNKTKSLDIQE"
     gene            complement(5669..6052)
                     /locus_tag="PK281_06660"
     CDS             complement(5669..6052)
                     /locus_tag="PK281_06660"
                     /inference="COORDINATES: protein motif:HMM:NF039344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="START-like domain-containing protein"
                     /protein_id="HOY49064.1"
                     /translation="MGGYKKIEIEYILKSSPGVLFSFLTEPSGLSEWFCDDVNIRSSV
                     YTFVWNKDSEQKAKLVSFKENVFVKYHWLDEPDETYFEFRIEVDDITGETAMTITDFC
                     EEDDEDETVLSWNSAMQRLHRVIGS"
     gene            6196..6939
                     /locus_tag="PK281_06665"
     CDS             6196..6939
                     /locus_tag="PK281_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOY49065.1"
                     /translation="MFNGKKVVVVLPAYNAALTLEKTYSEIPFEIVDEVVLVDDHSKD
                     NTSELGRKLGIQHIIRHEQNKGYGGNQKTCYAKALELNADVVVMLHPDYQYTPLLIHS
                     MVAIIANGLYPVVFGSRILGKGALKGGMPMYKYVANRFLTLSQNLLMNQKLSEYHTGF
                     RAFSGEVLRSLDFSHNSDDFIFDNEMIAQIFNKGFEIAEITCPTKYFEEASSINFKRS
                     SIYGLGVLKVSMKYMLHRNKIIRWNILSA"
     gene            complement(6940..7713)
                     /locus_tag="PK281_06670"
     CDS             complement(6940..7713)
                     /locus_tag="PK281_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOY49066.1"
                     /translation="MEILIPCIVALLASILTFFSGFGLGTLLLPAFAFFVPVQDAILL
                     TALVHMLNNLFKLILIGKHVAFDLALKFSLFTIPASFLGSYALDYLEDIKPIYAYMLG
                     SELHEITFVNVLIGLLMLVFAGFEVIPRWKAFQFPTKLLPVGAVLSGFFGGLSGHQGA
                     LRSAFLVRAGLSKEAFIATGIVIALVIDATRIPYYLYSFGQSSANNQTLLWILPTFCA
                     FIGAYAGSKLLKKVQIGFVQTTVAILLLVIALLLIFGIL"
     gene            complement(7738..>8239)
                     /locus_tag="PK281_06675"
     CDS             complement(7738..>8239)
                     /locus_tag="PK281_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49067.1"
                     /translation="PFKTQCAIKRLSRGTGQSKYDIAAQFSGFTQSLWWRTLYSPLIH
                     TDSAIYVYDHYRDSVFCFTEDGALDQKLEMKFHKDKTYKKQLIQDEVTKELYALHMHG
                     GTYTLAPVSYVDASEGNEIPLYYKYVEHIRIRNNEVFYVYRPFESSQNSFLYTEQLPL
                     RQLAGR"
BASE COUNT         2530 a         1767 c         1582 g         2360 t
ORIGIN      
        1 atcggggaag aactctccaa gtttttgatc cagtttgagt ttcttttcct cgattaactg
       61 atgaataatg actgatgtaa aaaccttgct gattgaacca attcggtatg cggtagaatc
      121 attacttctc acatgtgcca aagcttcggc ccaaccaaag cttcgttgat attcgatttt
      181 tccattcttg cggatacaga cagatcccat agctcgattt ttcttctgaa ctaaattgaa
      241 cagagaatca gctaattgaa aacgtgtgtc ttgtgctatg accggctttc cgatgtagca
      301 taaaaacacg aaactcagca gcagaatttt acgcatacca acgaaatcgg aaaagattag
      361 aaaaacttaa aaggaatgtc ccattttatc gcgctttgta gctagataac gggcattatg
      421 aatattggct tcaatcacaa ttggaacatt ttcaacaatc tccaacccgt aaccaattaa
      481 tccagcacgt ttggttggat tattcgttaa taatcgaagc ttgctcacac ctaaatctct
      541 tagtatctgc gctccaactc catagtcgcg ttcgtccatt ttgaaaccta acttgatatt
      601 agcttccacc gtgtcgtaac cttcttcttg aagtttgtag gctttgagtt tattcaatag
      661 tccaatgcca cgtccttctt gattcatata tacaatcacc ccttttcctt ccaattgaat
      721 ttgctgcata gctgcttgta actgtggacc acactcgcaa cgacaagaac caaaaatatc
      781 acctgtcata catgagctat gaacgcgcac taacaccggt tctccaggaa gaaattctcc
      841 tttcaccaga gccaaatgtt gctcacttgt attcgtttgg gtgtatgcaa ccatcttaaa
      901 atcaccatgt tcggttggta aatcaacttc cacttcacga cgaattaagc tatcgtgctc
      961 taagcgatat ttgattaaat cttcgatgga tataatcttc atatccaact tttcagcaag
     1021 ctgcaagagt tcaggtaaac gtgccatgct accatcctca ttcatgattt caaccaaaac
     1081 tcctgctggt tcaaaacctg ctaaacggga taaatcaatg gtagcttcgg tatgtccagc
     1141 acgacgcaat actccgccag ttttagcttt gagcggaaat atatgtccgg gacgagcaaa
     1201 atcttccggg cgaatcgatg gatcaatcaa agccttcata gtcttggaac gatcggacgc
     1261 agaaattccg gttgtacagc catgacccaa taaatcgacc gataccgtaa aagctgtatc
     1321 gtgagaagag gtgttttgag agaccatcat ttcgagtttg agctcctcac aacgttcttc
     1381 ggttagagca gcacaaataa gtccgcgacc ttgtgtagcc atgaagttaa ttacctcagg
     1441 agttgcattc ctagcggcag taaggaaatc accttcattt tcacgatttt catcatcaac
     1501 cacgataata attttaccct ggcggatgtc ctcaatggct tcctgaatac tgttaagttt
     1561 tgacatatag ctagcccaaa ggctgattaa gaaattagag aatcctgtcc ttgacagttg
     1621 atccggtgat caaacgattt aaccggttat tttgtttgtt ccaactgaat tcagcaacca
     1681 taaactcacg ggcattttga ccgattgtta gagctaattc agtattgttt aataagaaag
     1741 cgatggcttc tgcgactttt tcgggagtat cggcaatcca cacttcttta ccatgtcgag
     1801 caccaatggc gttattagct aaggtgctag taatgacagg caatcccatc gacatagctt
     1861 ccaaaatctt gttctgcatg cctattccaa tttgcatagg agcaacaaac aagcgtgttt
     1921 gctgataata gctacgtaaa tcgttcaccc aaccggtaag ttgcaaatga cgcgagttga
     1981 gttgtttcaa ttcacgactt ggattttttc ccgctgcaac aaactgaatg tgaggaaact
     2041 cctgatttaa tagcggtaaa acttggttca ccatatacac cacacaattc acattcggct
     2101 cgtattgcat attaccggtg aagagtaaat cgagtgttcg ggcacgtggt tcaggtttat
     2161 agaagtctgt atcaacacca ttaggaatga tacgtatttc ggctctttga ggatgattga
     2221 tcaagagtgc atcctgtgat gaaatcatcg tacgaatgtc gaacagatcg aaaatctcgg
     2281 tttcatagcg actcaatcgg ctactttcgg aagaaatcat catacgtttc caccaggcat
     2341 cgtgattcat tcttctttgc aaaccgatgg aaaatgcatc ttgataatcc agaatcttgc
     2401 gacaaccttg aagcgatttc acaaattcag ccatgcgagc taactgacaa taaaccacaa
     2461 caggcttaag ttgctgatac cattcctgaa actgacgaat tgcatcggca tcagagaaat
     2521 aagcaacttg atacggttta ctttggaaga aattcttggc aagtcgggct aaaactggaa
     2581 tacgttttaa ttgaaaaacg cgcacagaag caaaaaccga tttagccttt tcgagcgatt
     2641 catcagtaac agattcgtcg ctggtacaaa atacatgaac ctcgtgttcg ctagccaagc
     2701 cgagcagctg gtgccaggca cgaagcttat ctcctttgtc ggtaggaaac ggaaagcgtg
     2761 ataaggcaac aagtatcttc aaacccgagt acttattttt tcataaaact gaagaagccg
     2821 ttgggtcagc acctcagatc ggaaatgttc gttggcaaag gtaagggcat tttccgaaat
     2881 gcgcttagct agattaggtg atgtgagtag ttgtgacaga tgtgtcagaa aatcagctgg
     2941 tttatgagca acaaagaggt gttttccatc ctccgcagga ataccttcta agcctgttgg
     3001 tgtagttaat atgatacgcc caagtgccaa accttctata atcttcaccc gaattccgct
     3061 tccacttaac aaaggaacta tcataattcc ctccgattgc atgaactcag cagcatcggg
     3121 agcttgcttc acttcaattc cagctgcttg catttcttca tattcactcg gcatattctt
     3181 cccagctaaa accaatttcc aatcgggtaa tgctgcatgt aaactaggcc aaacctgatg
     3241 aataaaccat tcaacacctt cccgattcgg cagccaatcc attgctccca aatgaaagag
     3301 ttgcttcttt tctaatcccg tttgtgatgt caacggactt aaatcggttg ctatcccaat
     3361 aacttcaata ggaacatgaa cacccaattt tctaaatact tcagcatcca catccgacat
     3421 caccacaatc ccatcacaag cattacacaa atccaattct tcctttttca atcttctgga
     3481 aagcatgtgt agataagcct ttttgagggg attttgggct tgatttgcca tccgttccca
     3541 aatgagaaat tccacattat gagcccgtaa aatgattggt gctttactgg ctttgcgtaa
     3601 atcagcaata cctgtgaggg cgtaaatact ttcagcctga atgatatcaa ccggattttg
     3661 tttcaaccaa gcaacttgct gctgaattgc tgctgcatcg ttaaaccgac tcagattata
     3721 tgaatttcct tgaaataggt tgatgaatgc atcccaaggt tttactcgtg tatcggtaaa
     3781 atgatgctga atagtaaatg gataatcttt gggaatacta gctgggaatg gatgagttgg
     3841 tgtggaaatc atccattgag ttacggtatg cttggcttga tgtagtccgt tcgcaatcga
     3901 aaacatggca attttaccac cgtcgaaggg tgcaaacaaa ggtttggtgc agagttgcag
     3961 cacgttcatt cttggatgtc caaagactta gttttgttct tcatcacgaa tcgattccaa
     4021 aatcgtttgt aggcgtcggc atcgaataga gctgaatcgg tggtggcttt gtaactcaga
     4081 aaaataccaa tgggtaacaa tatcgctgat gaaagccaca ttccccagaa agcagtaagg
     4141 acgccttccc gagctagttt ttcaccggta atagaaatca tgtggtagag cacgaagaat
     4201 atcagggaaa atataactgg cattcccaat ccaccttttc gaacaatggc acctaaagga
     4261 gcaccaatga ggaagagaat taaacaggca aacgataagg taaacttcct atgccattcg
     4321 atttcatggc ggttgatgcg ttcctgatct tgctcaaaat tctgcatcga ataatcgatc
     4381 gtgcttttgt tggaacgtaa gttattcatg gctccatcca aaattttaat cttatcgttt
     4441 ttcgagaaat tatccagtac ctgagtttta gatttcaacg gaacattatt cgtgagaatc
     4501 ctcggttcat cagataaaaa agcaggtttc tgaattcctc gctcattcag aatttgcacc
     4561 gaatcgattc tgcctcttgc catcaatcga gctgtttcag ttcgcaatgc tatccgatgt
     4621 gttgtactat catgaagcgt gtctttgcta gtcgttgttt gagctttcgt tgtatcagct
     4681 ttagagcggt ttcgctttac taatttggga tctgtccaaa ctgtactata tgaacgcgta
     4741 gtatcgaaaa aaggtccatg caacaaaact ccgggcaaga agtgtttctc ttgtgcgagt
     4801 gcttctttca aggtatcagc cgataacatc aactgagtaa tgttaagcat ctgataatta
     4861 cgcttgtaaa cattctcgtc agtcttggtt agttcgaaaa tcgacaaatc gaatcgaatc
     4921 aaatcttctt gaaaattctc ccttagaaat ggataacgtc ctttttgttt acgtccaggt
     4981 tcggcttctg tatatgtatg accgttaacg agcgtaagtt cgaggtaacg tttatcgacc
     5041 gaaagaccca tatttcctcg ttcagcaatg ataacacgat cgttccccat acggttagaa
     5101 tgatcgtaaa tcatgatttg ttctagtttt ttcccgtcgt tacttttctt tccaatacga
     5161 atcacgtaat tctgaatatc gctattgaaa atacccggtt tgatattgag ttcgggacgc
     5221 ttgttggaga tatcctgcaa aagcgttttc attttcagat tggcaagcgg cagaatatta
     5281 ttagaaaagt aaaatgccga tatactcaag aaaagagcga aaatgagtac cgagcgcata
     5341 gcccgttgaa gcgaaattcc agatgatttc atggctgtta gttccatgta ttcacccaaa
     5401 ttaccaaacg tcattaaaga agaaagcaag actgcaagcg gcaatgctaa cgatatcatc
     5461 gttatagcaa ccaagccaag caacttgaaa atgataaacc actcaagccc tttaccgacc
     5521 aaatcatcca cgtacttcca aacaaattgc ataagcaaaa tgaaaagcgc gatgaaaaag
     5581 gtcaggatga acggaccgat ataagattta aatacaaagg agtcgattcg cttcaaggat
     5641 cgatacaatc aagccacaaa gataggcatc aagaaccaat cacacgatgc aaacgttgca
     5701 tggctgaatt ccagctaagc acggtttcat cttcatcgtc ttcttcacaa aagtcggtaa
     5761 tggtcatagc cgtttctccg gttatgtcgt cgacttcaat tcgaaattca aaataagttt
     5821 catctggctc atcaagccaa tgatacttaa caaaaacgtt ctccttaaag ctaaccagtt
     5881 tcgccttttg ttcagaatcc ttattccaaa caaatgtgta aacactgcta cgaatattca
     5941 catcgtcaca aaaccactcc gataatccac taggctccgt cagaaaagag aacaacacgc
     6001 ccggtgatga cttcagaata tattcgattt ctattttttt gtatcctccc attttcaggt
     6061 attggatgta taactattgg ttgcgattat acgaaaaggt aggttgccta caaattattt
     6121 cagataaaag tttgaaacat agtgttagaa atatagttga taaacagatg ctgaaaagat
     6181 tactaatttc gcggcatgtt taacgggaag aaagtcgtgg tggttttacc cgcctacaat
     6241 gctgcattaa cactggaaaa aacctattcg gagattccat ttgagattgt agatgaagtg
     6301 gttttggtag atgatcatag caaggataac acctccgaat taggtcgaaa acttggtatt
     6361 caacacatca ttcgtcacga acaaaacaaa ggatacggcg gaaatcagaa aacttgctac
     6421 gctaaagcac tcgaattgaa tgctgatgtc gtcgtaatgc ttcaccccga ctatcaatat
     6481 actcccctac tcatccattc gatggtcgca atcattgcta atggtttgta tcctgtagtg
     6541 tttggatcac gaattctcgg aaaaggtgcg ctcaaaggtg gaatgcctat gtataaatat
     6601 gtagcaaacc gctttctaac actcagtcag aatttattga tgaaccagaa attgtcggaa
     6661 taccataccg gttttcgtgc gttttcgggt gaagtgctgc gtagtttaga ttttagccac
     6721 aattcggatg atttcatttt cgacaatgaa atgattgctc aaatcttcaa caaaggtttc
     6781 gaaatagccg aaatcacttg tccgactaaa tatttcgaag aagcatcatc catcaacttc
     6841 aaacgttcgt cgatttatgg attgggtgtg ttgaaggttt cgatgaaata catgcttcac
     6901 cggaataaaa tcatccgctg gaatatttta tcggcttaat tacaaaattc cgaaaatcaa
     6961 gagtaatgca ataacaagca acagaatagc aactgttgtt tgaacaaatc ctatttgcac
     7021 tttcttgagc aatttacttc cagcataagc tcctataaag gcacaaaacg taggtaaaat
     7081 ccacagtaag gtttgattat ttgctgaact ctgcccgaat gaatacaaat aatacggtat
     7141 tcgagttgca tcaatcacca atgcaatcac gattccggtt gcaataaatg cttctttgct
     7201 cagtcctgca cggactaaaa aagcacttct caaagcacct tggtgaccac ttaatccacc
     7261 aaaaaatcct gataaaacgg cacctacagg aagtaattta gttgggaatt gaaatgcttt
     7321 ccaacgcggt atcacttcga aaccagcaaa tacgagcatt aacaagccaa tcaatacgtt
     7381 tacaaaggta atttcatgta attcgctacc caacatgtag gcgtaaatgg gtttgatatc
     7441 ttccaaataa tccaaagcat aactgcccag aaatgaagct ggaatggtaa acaaagaaaa
     7501 cttaagtgct aaatcgaagg ctacgtgttt gccaatcaat atcagcttaa acagattgtt
     7561 cagcatgtga accaatgctg tcaataaaat ggcatcctgc accgggacaa aaaaagcaaa
     7621 tgcaggtaaa agcaaagtgc ctaaaccgaa accactaaaa aacgtgagta tcgaggcaag
     7681 tagggcaaca atgcaaggaa tcaaaatctc cattgcccaa atatacaagc attcgaatca
     7741 tcttccagcc aactgcctga gcggtaattg ctcggtatag agaaaactat tttgcgaaga
     7801 ttcaaacggt cgatacacgt aaaacacttc gttattccga attcgaatat gttctacata
     7861 cttgtaatac aaaggaattt catttccttc ggatgcatcc acataagaaa ctggcgctaa
     7921 agtataggtt ccgccgtgca tgtgtaaagc atacaattcc ttcgttactt catcctgaat
     7981 gagctgcttc ttataggttt tatctttatg aaacttcatc tccagttttt gatctaatgc
     8041 accatcttcg gtaaaacaaa aaacactatc ccgataatga tcatatacat aaatagccga
     8101 atcagtgtgt atcaaaggtg aatacaaagt gcgccaccac agcgattgag tgaatcctga
     8161 aaattgagct gctatatcgt atttactttg tccggttcct cgacttaagc gcttgattgc
     8221 acactgagtt ttaaaaggc
//
LOCUS       DAOOQW010000094         8197 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_10793_length_8197_cov_5.519037, whole genome shotgun sequence.
ACCESSION   DAOOQW010000094 DAOOQW010000000
VERSION     DAOOQW010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 8197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8197
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10793_length_8197_cov_5.519037"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="PK281_06680"
     CDS             <1..205
                     /locus_tag="PK281_06680"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY49068.1"
                     /translation="NEQINILRSGSAKATLELCDVAGKIVMSSLLVDKVTSINTTQLE
                     AGTYMATIRQNGSVKTLKVLVIH"
     gene            360..626
                     /locus_tag="PK281_06685"
     CDS             360..626
                     /locus_tag="PK281_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009642311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HOY49069.1"
                     /translation="MKAEIHPSNYRLVVFKDMSDETLTLTKSCIDSKETIVWEDGNEY
                     PLAKLEISSSSHPFYTGKIKLVDTAGRVDKFRNRYKGHQSKINM"
     gene            693..1865
                     /locus_tag="PK281_06690"
     CDS             693..1865
                     /locus_tag="PK281_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014783147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlmU family protein"
                     /protein_id="HOY49070.1"
                     /translation="MNIILFDDSRRDHLLPLTFTRPIADLRIGILSIREKWEHHGFDR
                     ASYLTQDYLKAKFPSRRFAENLMIAGSIMPESDILDAVRRLSKGQALIQQGELIAAFD
                     TENSLSTKKENYECVEYKKPLFRIQHLWDIFSLNARALEDDFELLTKGRKSAEIDSSN
                     TIIGDRFFAEEGAKAWCSVFNTQSGPIYLGKDTEVWEGSMIRGGFALCEGSSVKMGAK
                     IYGATTVGPHSRVGGEVSNSVIQGYSNKGHDGFLGNSVIGEWCNLGADTNNSNLKNNY
                     STVKLWNYVEEKQISTGLQFCGLIMGDHSKCGINTMFNTGTVVGVSANIYGAGFPKNF
                     IPSFSWGGPQGITEYRLDKASETAALVMERRHKVFDDIESRLLEDVYELTRQYRQG"
     gene            1979..4471
                     /gene="lon"
                     /locus_tag="PK281_06695"
     CDS             1979..4471
                     /gene="lon"
                     /locus_tag="PK281_06695"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019670078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOY49071.1"
                     /translation="MNENTPFENFRFNPMIEDDSEFIPLLSSEDEEQMNTEEIPKEVS
                     ILPLRNTVLFPGVVIPITVGRDKSIKLIKDAYKADKIIGVVSQKDVGIEDPRFEDLNK
                     VGTIALIIKMLRMPDGNTTVIIQGKKRFQLEEVTQEDPYIKARIKAFEETRPAKDDKE
                     FAAMVSSLKDISLQIIQQSPNIPSEAAFAIKNIDSPSFLINFISSNMNAEVAEKQQML
                     EVADLKARAELVLKNLTKELQLQELKNQIQSKVKIDIDQQQREYFLHQQLKTIQEELG
                     GNVYEEELDEIRLKAGEMKWGDGVKKHFFKEMDKLQRINPNSAEYSVQRNYLDLLLEL
                     PWNKFTTDTFDLKGAQKILDKDHYGLEKVKERILEYLAVLKLKGDMRSPILCFYGPPG
                     VGKTSLGKSIAKALGREYIRMSLGGVRDEAEIRGHRKTYIGAMPGRIIQNLRKAKAAN
                     PVFVLDEIDKVGSDYHGDPSSALLEVLDPEQNSTFYDNYVEQDFDLSRAMFIATANNL
                     NTIQPALLDRMELIEVTGYTQEEKIEIAKQHLIPKQLEEHGVTKNQVKFSKKALDKLI
                     EDYTRESGVRALEKRIAKVIRSVAKSIAMDEVTNPKIDAEDVERIMGPARYHRDKYEN
                     NEVAGVVTGLAWTSVGGDILFIETLLSKGNGKLSLTGNLGDVMKESAVIALEFLKSHA
                     ESYLGISPEIFDHYNVHIHVPEGATPKDGPSAGIAMLTALSSAFTQKKVKSQLAMTGE
                     ITLRGVVLPVGGIKEKILAAKRANIQTVILSDENRKDVEEINSEYLKGMEFHYVSTMR
                     EVLKLALTNQLVKNPIKIELPKDKQKSTSSVN"
     gene            4474..5754
                     /locus_tag="PK281_06700"
     CDS             4474..5754
                     /locus_tag="PK281_06700"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOY49072.1"
                     /translation="MKASNNSDQPKQKLVVVGGGFGGIELIKRLRNSDFEITLVDKHN
                     YHTFQPLLYQVASGGLSADSIGYPFRLIFRNQKNFRFRMAEVQKVDSSKKQLETSTGI
                     IPYDHLVLATGSTSNYFGNTEVERYGRPLKSIPEALNLRSEILQELEKATLTAKHSDQ
                     KQHMTFVIVGGGPTGVETAGALAEFKKFVLPHDYPELDPKLMRVYLVEAGPRLLNGMS
                     EKSGNNAVKYLQNLGVEVLLNVAVLKYDGHKVVFSDQSELESTTLVWSAGVKGFIVSG
                     LPEESVKGNRYVVDEFNRVAGTDAIYSIGDSAGMVTTEKPRGFPMVAPVAVQQGTNLA
                     KNLLRLKDEKAWKPFQYKDKGSMATVGRNKAVVDMPNFSFGGFFAWLVWMFLHLMLLA
                     GFRNRVIVFVDWMWSYLTYERALRLIVRRAETPK"
     gene            complement(5762..6454)
                     /locus_tag="PK281_06705"
     CDS             complement(5762..6454)
                     /locus_tag="PK281_06705"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOY49073.1"
                     /translation="MVIQERKIKLALAEDQAVNRNTFIRKVDQMNGCELIFIANNGND
                     CLEKLNGLPENNKPDVLFMDIEMPGMNGIETIARAHLLYPSIHFLVLTVFDDDDKVFE
                     AIKAGAEGYLLKHESAQILEDAVHNVIEFGGVPMSPAIARKALKMLQRSEYYESHLQP
                     KHSSIPENISDRETEVLQLMVSGMDAKSISAHMNLSVHTVRKHIANVYEKLHVCSKAQ
                     IISLAHKQGWFQ"
     gene            complement(6493..>8197)
                     /locus_tag="PK281_06710"
     CDS             complement(6493..>8197)
                     /locus_tag="PK281_06710"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOY49074.1"
                     /translation="PVKVAYAQYNVAYAYYFSNANDSARLVIDEALKNLPAKTPEQRK
                     IYYKLKGFKATTYQGQRKNSEALQLLYPLLTEAEQNNDSLYIAQTMHQIAVIEGQQTN
                     PQKLIAWEQKALNVLPHSDTVSRIISATIFATIGKAYFQLNQPNYATFYHLKAIEIFR
                     REQDLYNLGVVLQRQSNVYIQDKKVSEAKKILDELSALNQQIKFGEGDINYYLSFIQY
                     WLLTGEYDRVIASCREHLYGENASPNESIRHSYLKALADAYKAQKNWAEYSLTIEEYI
                     QAKESFYQENSVEAIAEMETKYEVQKKENLIIQQEYDLVRKNFMFYGSLILLALVLLI
                     GWLIFTNYRRKSEHKLFFMQEEEKRHAQQAVKDAEEKERKRIAADLHDSLGSYAASIK
                     ANADEILTSNSISKSSLELLQSNSQQMVALLGDTIWALRKEKMNLSDISDRIKVFLNR
                     LRPNYPQVNMRVLEDLQNDWEFQPNQAFHLFMIVQEAVNNALKHSQGTEIKISINCKD
                     SWEIRINDNGRGFSKTESWNEDGNGLANMQTRAENMSCSIQWNQISPSGTEVCIRPTT
                     N"
BASE COUNT         2524 a         1570 c         1705 g         2398 t
ORIGIN      
        1 gaatgagcaa atcaacatcc tacgaagcgg ttctgctaaa gctactcttg agctttgtga
       61 cgtagccgga aaaattgtaa tgagctctct gcttgtcgat aaagtgactt ccattaacac
      121 cactcaactc gaagcgggaa catacatggc aacaattcgc cagaacggat cagtgaaaac
      181 actcaaagtt ttagtcattc attaagcatc aaagaaagta gttgtggtta agtttcaatt
      241 tgaaatacgc gtggcagtag cctaaccgct gtcacgtgta atttctaaaa ttcacaatta
      301 tttttcttgc ataagaaatc taatcatctt acatttgccg tccatttttg agaataccta
      361 tgaaagcaga aatccatcct tcaaattatc gtcttgtagt atttaaagac atgtcagacg
      421 aaacattgac tttaactaag tcatgcattg actctaaaga aacaattgtt tgggaagatg
      481 gtaatgaata tccactagcc aaattggaga tttcttcttc ttctcaccca ttctacacag
      541 gaaaaattaa actggtagat actgcaggtc gtgtggataa attccgtaac cgttacaaag
      601 gacatcagag caaaatcaac atgtgatttt actccaatat tcatcacaca aaagccttcc
      661 aaatggaagg cttttttatt tttgtcctac ttatgaatat tatcctattt gatgattccc
      721 ggcgagatca tttgctgcca ttaacattta cccgacctat tgcagacttg cgaataggta
      781 tattaagtat tcgagaaaaa tgggagcatc atggatttga tcgagcgtct tatttaacac
      841 aagattacct aaaggcgaag tttcctagtc gtcgttttgc tgaaaatcta atgattgcag
      901 gaagtataat gcccgaatca gacatattgg acgcagttcg aagattatca aaaggacaag
      961 ccttaataca acaaggagag cttatcgctg catttgatac agagaatagc ttatctacca
     1021 aaaaagaaaa ttacgaatgt gtggaataca agaaaccatt gtttcgaatc cagcatttat
     1081 gggatatttt ttcattgaat gcccgagcat tagaagatga ttttgaattg cttactaaag
     1141 gcagaaaatc ggccgagata gacagttcaa atactataat tggtgatcgt ttctttgccg
     1201 aagaaggcgc aaaagcatgg tgttcggttt tcaataccca atctggtcct atttatttag
     1261 gaaaagacac agaagtttgg gaaggaagta tgattcgcgg aggatttgct ttgtgtgaag
     1321 gtagttcggt aaaaatggga gctaaaattt acggagcaac aaccgtagga ccacattcac
     1381 gagttggtgg cgaggtaagc aacagtgtca ttcaaggtta ttccaacaaa ggacatgatg
     1441 gcttcctcgg aaattcagtt ataggagaat ggtgcaactt aggagctgat acgaataatt
     1501 ctaatttaaa gaataattac tctactgtta agctttggaa ttatgtagag gaaaagcaga
     1561 tttctaccgg attgcagttt tgcggattaa tcatgggtga tcacagtaaa tgtggaatca
     1621 ataccatgtt caatacgggt acagtagtcg gagttagcgc taatatatac ggagccggat
     1681 tccctaagaa cttcatccca tcctttagct ggggcggccc acagggaatt accgaatacc
     1741 gccttgacaa agcctccgaa accgctgcat tggttatgga aagaaggcat aaggtgtttg
     1801 acgatattga atcccgctta ttagaagatg tttatgaatt aacgcgacaa tatcgtcagg
     1861 gttaagtcgt atatttcatg tattattcct ttggcacata gcttgaccaa agtcgccccc
     1921 cgttggatat tcctgatttt gtgcggcagt ctgacgaaat gtcacttgat agaaatttat
     1981 gaacgaaaat acacccttcg aaaactttcg ctttaacccg atgattgaag atgattcgga
     2041 atttatcccg cttttatcga gtgaggatga agagcaaatg aataccgaag aaatccccaa
     2101 agaggtttcc atattaccct tgcgtaatac cgttcttttt ccaggagttg ttattcccat
     2161 tactgttgga agagataagt cgataaaact cattaaagac gcctacaaag ccgataagat
     2221 tattggagtt gtttcccaga aagatgttgg gatagaagat cctcgttttg aagatttaaa
     2281 taaagttgga accattgcgc ttatcataaa aatgttgcgt atgcccgatg gtaacactac
     2341 cgttatcatt caaggtaaaa aacgcttcca gctcgaagag gtaactcagg aagatccata
     2401 tatcaaggct cgaatcaaag cattcgaaga aactcgtcca gcaaaagatg acaaagaatt
     2461 tgctgccatg gtgtcttctt taaaagacat ctcattacaa atcattcagc aatcgccaaa
     2521 tattccttcg gaggcggctt ttgcaattaa gaatatcgat agtccatctt tcctcatcaa
     2581 ttttatctct tccaacatga atgcggaagt agctgaaaaa cagcagatgt tggaagtggc
     2641 cgatttgaaa gctcgtgctg aattagtgtt gaaaaatctc actaaagaac ttcaactaca
     2701 agagcttaaa aaccaaatcc aaagcaaggt taagattgat atcgaccaac aacaacgcga
     2761 gtatttcctt catcagcaat taaaaactat ccaagaagaa ttgggaggaa acgtttatga
     2821 agaagaactc gatgaaatcc gcttaaaagc tggagaaatg aaatggggtg atggggtgaa
     2881 aaagcatttc ttcaaggaaa tggataagct tcaacgcatc aacccaaact cagctgaata
     2941 ttccgtacag cgcaattacc tcgatctatt gctcgagttg ccttggaata agttcacaac
     3001 cgatacattc gacttaaaag gcgcccaaaa aatcctcgat aaagatcact acggtctcga
     3061 aaaagtgaaa gaacgaattt tagaatacct cgctgttctg aaactcaaag gcgacatgcg
     3121 ttctcctatc ctttgctttt atggtcctcc aggagttggt aaaacatctt tgggtaaatc
     3181 aatcgcaaaa gcacttggtc gtgaatacat tcgtatgtca ttaggtggag tacgtgatga
     3241 ggctgaaatt cgtggtcacc gcaagacata cattggtgcg atgcccggac gtatcatcca
     3301 aaatttacgc aaagcaaaag ctgccaatcc tgtttttgta ttggatgaaa tcgacaaagt
     3361 aggaagcgac tatcacggag atccttcatc tgcattgcta gaagtattgg atccagaaca
     3421 aaacagcacg ttctacgaca attacgtaga gcaagatttt gacttaagtc gtgctatgtt
     3481 cattgctaca gctaataacc tcaacactat ccaacctgcc ctactcgacc gaatggaatt
     3541 gattgaagtt actggatata ctcaagaaga aaagattgag attgccaaac agcatttaat
     3601 tccaaaacaa ttggaagaac atggtgtaac gaaaaatcaa gtgaagtttt cgaaaaaagc
     3661 gctggacaaa ctaatcgaag attatacacg tgaatcgggt gtgcgcgctt tagagaaacg
     3721 tatagctaaa gtgattcgtt cggtagctaa atcgatagca atggatgaag tgactaaccc
     3781 taaaattgat gcagaagatg tagaacgcat catgggacca gcacgctatc accgcgacaa
     3841 atacgaaaac aatgaagtag caggtgtggt aactggtcta gcttggactt cggttggtgg
     3901 cgacatctta tttatcgaaa cgctactcag caaaggaaac ggtaaactat cgctcactgg
     3961 aaatttaggt gatgtaatga aggaaagtgc tgtaatcgcg ctcgaattct tgaagtcgca
     4021 cgccgaaagc tacttgggaa tttcaccgga gattttcgat cattataacg tgcatattca
     4081 cgttcctgaa ggcgcaactc ctaaagatgg accatcggct ggtattgcta tgttaacggc
     4141 tctttcttcg gcctttacgc agaaaaaagt gaagtcgcaa ttagccatga cgggtgagat
     4201 tactttacgt ggtgtagtat taccggttgg tggaattaag gagaagattc ttgccgctaa
     4261 acgggcaaac atccaaaccg ttattctttc ggatgaaaac cgaaaagatg ttgaggaaat
     4321 caactctgaa tacctcaaag gaatggagtt ccactatgtt tctactatgc gtgaagtgtt
     4381 aaagctagct ttgaccaatc aattggtgaa gaatccaatt aaaatagaac ttcccaaaga
     4441 caaacaaaag tcaacctcaa gcgttaattg agtatgaaag cctctaataa ttccgaccaa
     4501 ccgaaacaaa aacttgttgt ggtgggagga ggatttggag gtattgaact catcaagcga
     4561 cttcgaaact ctgattttga aattacatta gtcgacaagc acaattacca tacgtttcag
     4621 ccgctcttgt atcaggtggc atcgggaggt ttgagtgcag attcgattgg ctatccgttt
     4681 cggttgattt ttcggaatca gaagaatttc cgatttcgaa tggcggaagt gcagaaagta
     4741 gattcgagta aaaaacagct cgaaacttct accggaatca ttccctatga ccatttagta
     4801 ttagcaacag gttcaacatc gaactatttt ggaaataccg aagtggaacg ctatggccgt
     4861 ccactgaaaa gtattcctga agcattaaac ttaagaagtg agattcttca agaattagaa
     4921 aaagccacct taactgccaa acattccgat caaaagcaac acatgacttt tgtgatagtt
     4981 ggtggcggtc ctacaggtgt tgaaacagca ggagctttag ctgaattcaa gaagtttgtc
     5041 ctaccacacg attaccccga gctcgaccca aaattgatgc gtgtttattt ggttgaagct
     5101 ggaccacgcc tgctaaatgg catgtcggag aaatcgggta acaatgctgt caagtacttg
     5161 caaaatctcg gagtagaggt tttgttaaat gtagcagtat tgaaatacga tggacacaaa
     5221 gtagttttta gcgatcagag cgaattggaa tccaccactt tagtttggtc ggcaggagtg
     5281 aaaggcttta tcgtttctgg attgccggag gaatcggtga aaggaaatcg atatgtggta
     5341 gatgagttca atcgagttgc tggaacggat gcgatttatt cgataggtga ttcagctgga
     5401 atggtaacaa cagaaaagcc gagaggattt ccaatggttg ctccagttgc tgttcaacaa
     5461 gggacgaatt tggctaaaaa tctccttcga cttaaggatg aaaaagcttg gaaacccttt
     5521 caatacaagg ataaaggttc gatggcaaca gttggtcgga acaaggctgt agtagatatg
     5581 cccaacttta gtttcggagg atttttcgct tggttagtgt ggatgttttt acacctcatg
     5641 ttgttggctg gattccggaa ccgagtaatc gtatttgtcg attggatgtg gagttatctc
     5701 acgtatgaac gagcattacg cttgattgta cgtcgcgccg aaaccccgaa ataatcttgt
     5761 tttattgaaa ccaaccttgc ttatgagcca aactgatgat ttgggcttta gagcatacat
     5821 gtagcttttc ataaacgttt gcgatgtgct ttcgaacagt atgcacgctt aagttcatat
     5881 gtgcagaaat gcttttagca tccattccag agaccataag ttgaagcact tcggtttcgc
     5941 ggtcgctgat gttttcggga atactcgaat gttttggttg taaatgtgac tcataatatt
     6001 ccgaacgctg aagcatcttc aaggctttac gcgcaatagc tggactcatc ggaacgcccc
     6061 caaattcaat cacattatga accgcgtctt ccaaaatttg agcgctttcg tgtttcaata
     6121 aatagccttc tgcacccgct ttaatagcct caaaaacttt gtcatcatca tcaaaaacag
     6181 tcaacaccaa aaaatgaatc gaaggatata gaagatgtgc tcgagctatt gtttcaatcc
     6241 cattcatgcc aggcatttca atatccatga aaagtacatc cggcttgttg ttttcgggta
     6301 aaccattcag cttttcgagg cagtcgttac cattatttgc aatgaaaatc agttcacatc
     6361 cattcatctg atccactttg cgaatgaatg tattgcgatt tacagcttga tcttcggcga
     6421 gtgctaactt gattttacgt tcctggatta ccatatatca aatttgaata gaatcctctg
     6481 gttaggaaat aactaatttg tagtagggcg aatacatact tccgttccgg atggacttat
     6541 ctgattccat tgaatagaac aactcatatt ctcagcacgg gtttgcatat tggctaagcc
     6601 atttccatcc tcattccatg attctgtttt actgaagcct cgaccattat cgttgattcg
     6661 gatttcccag ctatccttac aatttatact aatcttaatt tctgttcctt gactgtgttt
     6721 gagcgcatta ttgactgctt cctgcacaat cataaatagg tgaaatgctt gattgggttg
     6781 aaattcccag tcgttttgta aatcttctag cacacgcata ttcacttgag gataattcgg
     6841 tcgaagacgg tttaggaaga ctttaattcg atcgctgata tcactcagat tcatcttttc
     6901 ttttcgcaaa gcccaaatgg tatcacctaa aagagcaacc atttgttgag aattggattg
     6961 tagaagttct aaacttgatt tagagatgga gttgctagtt aaaatctcat ctgcattagc
     7021 tttaatactg gcagcataac ttccaaggct atcgtgcaaa tcggctgcga tacgtttacg
     7081 ttccttttct tctgcatctt ttacggcttg ttgagcgtga cgtttttctt cttcctgcat
     7141 aaagaagagt ttatgctccg attttcgacg gtaattagta aaaatcaacc aaccaattaa
     7201 gagcactaat gccaataaaa taagtgaacc gtaaaacatg aagttcttgc ggaccaaatc
     7261 gtattcctgt tgaatgatga ggttttcctt cttttgaacc tcatattttg tttccatttc
     7321 ggctattgcc tccaccgaat tttcctgata gaaactttct tttgcctgaa tatattcttc
     7381 aatagttaag ctgtattcag cccagttttt ttgagctttg tatgcatcag caagtgcttt
     7441 caaataagaa tgacgaatgc tttcgtttgg acttgcattt tcaccatata aatgctcccg
     7501 acagcttgca ataacacgat cgtattcgcc tgttagcaac caatattgaa taaaactgag
     7561 gtaataattg atatcgccct cgccaaattt tatttgctga tttaaagcag aaagttcatc
     7621 tagaattttc ttggcttcgg acactttctt atcctggata tatacatttg actggcgctg
     7681 taaaacaact cctagattat ataaatcctg ttctcttcga aaaatttcga tggcttttaa
     7741 gtgataaaag gtagcataat tgggctgatt gagttggaaa tatgctttcc cgattgtagc
     7801 aaatatggta gcagatataa tgcgagaaac ggtgtcgcta tggggtaaaa cgttcaatgc
     7861 tttttgctcc caagcaatga gtttttgagg attagtttgt tgtccctcaa taacagcaat
     7921 ttgatgcatc gtttgagcaa tgtataagct gtcgttattc tgctcagcct ctgttaacaa
     7981 aggatacaac agttgcaggg cttcggaatt tttgcgttgt ccttgataag tggtggcttt
     8041 gaatcctttc agtttgtaat agatttttcg ttgctctgga gttttagcgg gaaggttttt
     8101 taaggcttca tcaatgacca aacgagcgct atcgttcgca ttagagaaat aatacgcata
     8161 agctacatta tactgagcat aagcaacttt cacagga
//
LOCUS       DAOOQW010000095         8191 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_10805_length_8191_cov_3.789577, whole genome shotgun sequence.
ACCESSION   DAOOQW010000095 DAOOQW010000000
VERSION     DAOOQW010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 8191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8191
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10805_length_8191_cov_3.789577"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            171..908
                     /locus_tag="PK281_06715"
     CDS             171..908
                     /locus_tag="PK281_06715"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HOY49075.1"
                     /translation="MVHLVLDIGNTALKAACFQGRTLLKASRTAWTSPQEIEESLLRM
                     IALNPSKVLISSVTGKKKEVDDFLKRAGLEQDYFNPKTPVPISNKYETPETLGRDRLS
                     NAVAATVMFPQLPVVVIDAGTCIKFDFITRNNEYFGGSISPGIDMRFRALHEFTARLP
                     LLSRAEMVYLIGKNTTESIHSGVINGALAEVKGILEQYQMTHKDLQVIITGGDYTLLE
                     RNLKTSVHIEPWLTLKGLNEILLHQAR"
     gene            880..2286
                     /locus_tag="PK281_06720"
     CDS             880..2286
                     /locus_tag="PK281_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49076.1"
                     /translation="MKFFSIRHASSIFFLSCFLAPACTIQAQTLSQSPYGRFGLGDHV
                     GNSSAYIQGIGGSTQAVSDSNSLNLSQPASLASLEGGVTILEFGFSGLTSKYQGLQNT
                     KMGRTAGFGYFGLAFPIMKNKWNLSLSLTPLTNAGYTLRDSILDDEIGKINLNYKGQG
                     GFSAFTIGNGFHIGKNLNLGVQLQYLFGKTDYSSEVLYSGDTITQRNSLVTNSNRLSD
                     VDLVFGAQYLIRFHKIKSTVKSSSDSSQVSARKARLRNDSLHVLLGGTFRPATFINGS
                     TSYLAQTFFGNTPGTSYTDTVLFKDQEKGTVAMPMQVGGGITFGSSSRSWMLTTEYIH
                     TDWNGFRLFDRIDSVRTSYRIGMGLEINPTPINRSTSAKTAYWKKIRYRVGGYYSDGY
                     LHINGQAIPEFGVSLGFGLPITIRTYNTRTATSVLNFAVTAGQRGVSGTNSLQEQFIR
                     GTVSFSLNDRWFRKFQYD"
     gene            2279..2818
                     /gene="lptC"
                     /locus_tag="PK281_06725"
     CDS             2279..2818
                     /gene="lptC"
                     /locus_tag="PK281_06725"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HOY49077.1"
                     /translation="MTKLYCIFVGLLLLFMYSCGNDLNEVKKFANEERVPISSTRNVE
                     MLYSDSARLRAKINAPLRETYVGKDGFIEFPKGIKVEFYGVNSKLESKLKANYAKSYT
                     GQERMEAKNNVVIESTSGDKLETEHLIWDQREDRIFSNVFTRITSPDRVIYGDGFESN
                     QDFSKYRILKVKGIINLNE"
     gene            2849..3061
                     /locus_tag="PK281_06730"
     CDS             2849..3061
                     /locus_tag="PK281_06730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49078.1"
                     /translation="MELVWLSIAIMAAISSTWLITKGDFDAARFPLFVAFCAAILFAL
                     RRYQRKRIEKVEAFEKENPKSTTTKK"
     gene            3153..5285
                     /locus_tag="PK281_06735"
     CDS             3153..5285
                     /locus_tag="PK281_06735"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HOY49079.1"
                     /translation="MAVLERIRSKAGTIVVVVIGLALFSFVMQDLFSNGSSIFSGSDR
                     EIGEINGHSVDGEEFSRRLSKAEDNVKQSQGTSGVDDNTRQQLVNQIWNEYLDEYLFN
                     EQMEEAGVAVSDDEIFDMIQGENIHPQVMQIPLFQDSLTKQFDRNKVLKFLQTQLSEE
                     NDPDGKYRESWAEFEKSIAKQRLKTKYNNLLRKAVYVTKAQAKFDFNNKNTAAAYRVI
                     VKKYDTVADSTITVTDEEMKKYYDEHNFEFEQKEETRKLEYVVFQVNPTPEDRAELFK
                     SMEELKAGFMASTDDTSFVNANSSEGFRIESIKRGKIAPQIDSAVFSGTPGSVYGPFA
                     DDASLKLVKLQGFKATSDSVMARHILISTQGGMSIAAATAKADSLKAAIKAGADFSLL
                     AMQFSEDPGSKTKGGDLGWFTEGTMVPEFNDACFNGNVGDMVTVNTQYGAHLINITNK
                     TRSSNKAQVVYLSKKIEPSTRTVDEYYAKANDFAVAAQDYDAFKKQAEEKGMFIVSYD
                     RLLPNDRQVNDLTNSREMVQWAYNPERALKEVSKVFDIEGRYVVASLTGIKNKGIPPF
                     DQLKETLKPLAIREKKAATFTKEFSDAMAGATTIEALAGKMNLQLDTVDATLTFSTNS
                     IPMFGIEPQVIGRTFSLPVKKLSKPIKGNTGVFVVVVNSITPGVLPTDLTETRKQYAQ
                     SMSGRVENSVYNALIKKGNVEDKRYRFF"
     gene            complement(5604..6596)
                     /locus_tag="PK281_06740"
     CDS             complement(5604..6596)
                     /locus_tag="PK281_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49080.1"
                     /translation="MNLKTSIYLFALLLLSNSGINVHAQKVDALYQGSDIDSSYYDIY
                     QVAENDIWIGGEYGVLKRLKQDGSLSNISIPNNGSNILKFLRLGDFVYISADHGTIYK
                     YNLKDSTCTKTEFHAFRHRCFYDLSYDNQGHLVVCGGSSGIGRGKKRIPNGFIATIDT
                     SLAQQPQVIWKNTRKFVWALANEGESGFAAAVFNGVSTTIYHHNPNQSADLVKGRKVK
                     GLIHALKSIDGQMAYSGCRSIRYHRSGIWGFENNPNSHTVVKNAGIICNLVQIKGRIY
                     GFSQQGILYALNGNEAEQMLQASNASAFYEALLVGSETLYIAGHGKSLIKVTLD"
     gene            complement(6647..8068)
                     /locus_tag="PK281_06745"
     CDS             complement(6647..8068)
                     /locus_tag="PK281_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49081.1"
                     /translation="MSLRILLLFSLFLVLPLGAQQIKPISTDSIEMVIQGALRFIEQE
                     QVKKTIPEHQYEGEWPAFMTMRSSFVMLGKQERSYDSNCFSNASIQNSLSAAFLIRPD
                     LTSIPPMLDKAIIRMMDFYSGNGFNFWPLLPPNGKLYKFKKNQENGMVRRPIRFPLNN
                     PYIRKAANVVNDNDDTSQGFSALMNYVKVQKTLGNKVDVSFRISPILGAWRDSARSSQ
                     HYYNIIHFDKRESGAFLTWRAQEIPFPNWNVPRMLVNNAFFLTPLSTLYPRAFVPYIP
                     YGCNDVDAVVNANVLTALAENGETKAAGVSKASKFIERKIRRKDWSRAGIYYPNRYHL
                     HYATLKAWSKGIRDLDESALRLKKHLLSSQYADGSFHSRRIVNHKDKVQSTAYALNSI
                     LRLGNPYLIGTEIQVNNAIHYLINQASFMDGNTCWDGGVFFSGGTVIRNTLYWKSDVY
                     TTALMVESLVLYQQYVKEIKTFW"
BASE COUNT         2545 a         1532 c         1597 g         2517 t
ORIGIN      
        1 accagggaca catggatttt cagtccattg ctctaccatc tgagctaagg caccaagcct
       61 ttcttgctgt tgagcgggtg caaaaatagt ttttttagtt tctcagcaaa aaattttttt
      121 acgctttggg cgtttttcta attctatctt tgcggctgaa actattcaga atggttcatt
      181 tagttctcga catcggtaat actgctctca aagcagcttg ttttcagggg cgtacgcttt
      241 tgaaggctag tcgtacagct tggacttccc ctcaagaaat tgaagagtca cttttaagaa
      301 tgattgcatt aaatccttcg aaagtgctca tcagttcggt aactgggaag aaaaaagagg
      361 ttgatgattt tttaaaacga gcaggattgg agcaggatta tttcaatccc aaaacacctg
      421 tacctatcag taacaaatat gaaactcccg aaacattggg gcgtgatcgt ttgtcgaatg
      481 cagttgctgc tactgtgatg tttcctcaac tacctgtcgt ggtgatagat gctggtacat
      541 gtatcaaatt cgatttcatt actcgaaaca atgaatattt tggaggatct atttctccag
      601 gaattgacat gagatttcgt gctttgcatg aatttaccgc tcgtttgcct ttacttagcc
      661 gcgccgaaat ggtttatctc attggtaaaa acaccactga atcgatacat tcgggtgtaa
      721 ttaacggtgc tttggctgaa gtgaaaggaa tcttagaaca ataccaaatg actcataaag
      781 accttcaagt aattattact ggaggtgatt acacgctatt agaacgaaat ttgaaaacca
      841 gtgttcatat tgaaccttgg ctcacactta aaggactcaa tgaaattctt ctccatcagg
      901 cacgctagtt ctatcttttt cctgtcgtgc ttcctggcac ctgcttgtac tattcaagca
      961 caaacactta gtcaatctcc atacggacgt ttcggattgg gcgatcatgt gggtaattca
     1021 tccgcttaca ttcaaggtat tggaggaagt actcaagctg tatccgattc taattcgctc
     1081 aatttatctc aacctgcttc tttagcttct cttgagggcg gtgtgacgat tttagagttt
     1141 ggcttttcag gattaacttc taaatatcaa gggcttcaaa acaccaaaat ggggcgtacg
     1201 gctggatttg gatattttgg tttggcattt cctatcatga aaaataagtg gaatctttca
     1261 cttagcttaa ctcccttaac aaatgctggt tacacattac gtgacagtat attggatgat
     1321 gaaataggaa aaatcaatct gaattacaaa ggacaaggtg gtttttcagc atttaccata
     1381 ggaaatggtt ttcatattgg gaagaatttg aatcttggcg ttcaacttca atatttattc
     1441 ggaaaaacag actatagttc tgaagtatta tactcaggtg atacaattac tcaacgtaat
     1501 tcactggtta ctaattcgaa tcgcttgagt gatgtggatt tagtctttgg tgctcaatac
     1561 ctaattcgat tccacaaaat aaaatcgact gtcaaatcta gttcagactc ttctcaagtt
     1621 tctgcccgaa aagctcgact tagaaacgat tctttacatg ttttgttagg tgggacattc
     1681 cgtccagcaa cattcattaa tggttcaaca tcgtacttag cacaaacatt ttttggtaat
     1741 acgcctggta catcttatac cgacacggta ttatttaaag atcaggaaaa aggtactgtt
     1801 gctatgccta tgcaagtagg aggaggaatt acatttggaa gtagcagtcg ttcttggatg
     1861 ctaactacag aatatatcca tacagactgg aatggtttcc gattatttga tcgtatcgat
     1921 tccgtaagaa catcctatcg tatcgggatg ggcttagaga ttaatccaac tcctataaat
     1981 cgcagcactt cagccaaaac agcctattgg aagaaaataa gatatcgggt tggtggatat
     2041 tattcggatg gatatttaca catcaatggt caagctattc ctgaatttgg agtgagttta
     2101 ggttttggat tacctatcac tattcgaacg tataatacac gtactgcaac tagtgtattg
     2161 aactttgcag taactgccgg tcagcgtggt gtttctggaa cgaattcgct tcaagaacaa
     2221 ttcatccgag gaacagtatc tttttcactg aacgacaggt ggttcaggaa attccagtat
     2281 gactaagctg tattgcattt ttgttggatt attactgttg ttcatgtatt cctgtgggaa
     2341 tgatttgaat gaggtaaaga aatttgccaa cgaagagcga gttccaattt cttcgactcg
     2401 aaatgtggag atgctctatt cggattcggc acgattgcga gcgaagatta atgcaccgtt
     2461 gagagaaacg tatgttggga aggatggatt cattgagttt cccaaaggca taaaagtgga
     2521 gttttatggt gttaactcca agttggaatc gaagttaaaa gccaattatg caaagagtta
     2581 taccggacag gagcggatgg aagctaagaa taatgtggta attgaaagta caagtggaga
     2641 taagcttgaa acagagcatt taatttggga tcaacgcgag gatcgtattt tctcgaatgt
     2701 attcacacga atcacttcac ccgatcgagt aatatatggt gatggttttg agtcgaatca
     2761 agacttcagc aaatacagga ttttaaaagt caaaggaatc atcaatttga atgaataaaa
     2821 tatgaatcaa gcacgcattt ttcgaatcat ggagcttgtc tggctcagca ttgcaatcat
     2881 ggcagctata tcgagtactt ggctaatcac caaaggagat tttgatgcag ctcgttttcc
     2941 attgtttgtc gctttttgtg ctgctatatt attcgcttta cgacgttatc agcgcaaacg
     3001 cattgagaaa gtagaagcct ttgaaaagga aaatcctaag tcaaccacca cgaaaaaata
     3061 agcatacagg cacaaaatca accatattta ggtttttttt gatttagtcg tatattcgca
     3121 cccctaaaaa accaaagatt tagcttccac aaatggcagt tcttgaacgc atccgctcca
     3181 aagccggaac aatcgtagta gtagttattg gtcttgcact cttctcattc gttatgcaag
     3241 atttattttc aaatggaagt tccatcttct ctggatcaga tagagaaata ggtgaaatta
     3301 acggtcattc cgttgatggc gaagagttta gtcgccgctt atcaaaggcg gaagacaatg
     3361 ttaaacagag ccaaggaact tctggcgttg acgataatac acgtcagcaa ttagttaatc
     3421 aaatctggaa tgaatactta gatgagtatt tgtttaacga acagatggaa gaagctggtg
     3481 ttgctgtatc ggatgatgaa attttcgata tgattcaagg agaaaacatt cacccacagg
     3541 tgatgcaaat ccctttgttt caagacagct taactaagca atttgatcgt aacaaggtgc
     3601 ttaagttttt acaaacacaa ttgtcggaag agaacgatcc tgacggtaaa taccgtgaat
     3661 catgggctga attcgaaaag tcaattgcta aacaacgtct taaaaccaag tataacaact
     3721 tgctacgtaa ggcagtttat gtgactaaag cacaagcgaa atttgatttc aacaacaaaa
     3781 acacagctgc tgcttatcgt gtcattgtta agaaatatga tactgttgcc gattcaacaa
     3841 tcacggttac agatgaagaa atgaaaaaat actacgacga gcataacttc gaatttgaac
     3901 aaaaagaaga gactcgtaaa ttggaatatg tagtatttca agtgaatcca actccagaag
     3961 accgtgctga attattcaaa tcgatggaag aattgaaagc aggtttcatg gccagtactg
     4021 atgatacttc ctttgttaat gcaaattcat ctgaaggatt ccgtattgaa agtatcaaac
     4081 gtggaaaaat cgctcctcaa attgattctg ctgtgttcag tggaactcca ggttcggttt
     4141 atggcccgtt tgcagatgat gcatctttga aattggtaaa gcttcaagga tttaaagcta
     4201 cctccgattc agttatggct cgtcacattc ttatttctac acaaggtggt atgagtatag
     4261 ctgcagctac tgcaaaagct gattcgttga aagctgctat taaggcaggt gcagacttct
     4321 cacttcttgc catgcaattc agtgaagatc caggttctaa aacaaaaggt ggagatttag
     4381 ggtggtttac cgaaggaacg atggttcctg aattcaacga tgcttgcttc aatggtaatg
     4441 ttggtgatat ggttaccgtt aatacacaat acggtgctca cttaatcaat atcaccaata
     4501 aaactcgttc ttctaacaaa gctcaggtag tttacttgtc taagaaaatc gaaccaagta
     4561 ctcgtacagt tgatgagtat tatgctaaag caaatgattt tgcagtagct gctcaagact
     4621 acgatgcatt taaaaaacaa gctgaagaaa aaggaatgtt tatcgtttca tatgatcgtt
     4681 tgcttccaaa tgatcgtcag gttaacgatt taactaattc tcgtgagatg gtacaatggg
     4741 catataaccc agaacgtgca ttgaaagaag tatcaaaagt attcgacatc gaaggtcgtt
     4801 atgttgttgc ttcccttact ggaattaaga ataaaggaat tcctcctttt gatcaattga
     4861 aggaaacctt gaaaccactt gcaattcgtg agaagaaagc agccactttc actaaagaat
     4921 ttagtgatgc tatggctggc gctactacta ttgaagcttt agctggtaaa atgaatttgc
     4981 aacttgatac tgttgatgcc acgctaactt tctcaacgaa ttcaattcca atgtttggaa
     5041 ttgaacctca agtaattgga agaacattct ctttgcctgt taagaaatta agtaagccta
     5101 tcaaagggaa tactggtgta tttgtagttg ttgttaattc aatcactcct ggagttcttc
     5161 caaccgactt aactgaaaca cgtaaacaat acgcacaatc aatgtctgga cgtgttgaga
     5221 acagtgttta caacgcttta attaagaaag gtaatgtaga agataaacgc taccgtttct
     5281 tctaaaccga tataatcgca ttttgaaacg cgcttcttaa ttggagcgcg tttttttttt
     5341 gccctctttt tctaccgatt gaatgattga ttgaatttgt atgtggtgtt tgatttgtaa
     5401 aagttgaatc aaccgtttta attcacccga ataattcagg attaacaaca tcgctttagc
     5461 agcttatagt tgctctaact tgcttaaata actatattgt attgatattc tagggatagt
     5521 ttagtctaaa cctttgtttc aatcctagaa aaagaaaatt gacagggagt tttgatgctt
     5581 atcagttagc aaacacaaga aaattaatca agtgttactt tgataaggct tttgccatga
     5641 ccggcaatat aaagtgtttc cgaaccaact aaaagtgctt cgtagaaagc agatgcgttt
     5701 gaagcttgca acatttgttc tgcttcgttc ccattaaggg catatagaat tccttgttga
     5761 ctgaaaccat aaatgcgacc tttgatttga acgaggttgc agataattcc ggcattcttt
     5821 actactgtat gcgagttagg attattttca aagccccaaa tgccacttcg gtggtaacga
     5881 atagaacgac aaccactata agccatttgt ccatcgatcg atttcaatgc gtgaatgagt
     5941 ccttttactt tgcgaccttt aactaaatcg gccgattgat ttggattatg atgatagatt
     6001 gtggttgaaa caccattgaa aacggctgct gcaaatcccg attcaccttc gttagctaat
     6061 gcccaaacaa actttcgtgt gtttttccaa atcacctgag gttgttgtgc aagcgaagta
     6121 tctattgttg caatgaagcc attaggaata cgttttttac cgcgccctat accactactt
     6181 cctccacaaa caactaaatg accttgattg tcatacgaaa gatcataaaa acaacggtgt
     6241 cgaaatgcgt ggaattcggt ttttgtgcag gttgaatctt tcagattgta tttgtaaatc
     6301 gtcccgtgat ctgccgatat gtaaacaaaa tcacccaaac gaaggaattt caaaatgttt
     6361 gatccgttgt taggaataga aatattgctt aatgaaccat cctgtttcag acgttttaat
     6421 actccatatt caccaccaat ccagatatcg ttttcggcaa cttgatagat atcgtaataa
     6481 ctactatcga tatcactgcc ctgatacaaa gcatctacct tttgtgcatg cacatttatc
     6541 ccgctattag atagcaagag tagggcaaac aaataaatag acgttttcaa attcacaggg
     6601 cgtaaaacta aacaattagc acgaagcaca aacctatacc gattacttac caaaaggttt
     6661 taatttcttt cacatattgt tgatataata ccaaggattc taccatcaat gcagttgtat
     6721 atacatccga tttccaatat agcgtattcc ttattaccgt tcctcccgaa aagaaaactc
     6781 ctccatccca acaggtattt ccatccataa atgaagcctg attgattaag taatgtatcg
     6841 cattgttgac ttgtatctca gtaccaatca aatatggatt tcctaaacgt aaaatggaat
     6901 tgagcgcata agctgtcgat tgtactttgt ctttatgatt tacgattctg cgcgaatgaa
     6961 aagaaccatc agcgtattga ctacttaata aatgcttttt taagcgcaaa gcactttcat
     7021 ctaaatctcg aattcctttc gaccatgctt taagggtggc ataatgtaaa tggtagcgat
     7081 tgggataata tattccagct cgactccaat cttttcgtct tattttccgt tctataaact
     7141 ttgaagcttt tgaaactccc gcagctttag tttccccatt ttcggctaag gcagtcaata
     7201 cattcgcatt tactacagca tcaacatcat tgcatccata gggaatatag ggtacaaaag
     7261 ctcgaggata taatgtactg agtggagtta agaaaaaagc attattgacc aacattcttg
     7321 gtacattcca attggggaat ggtatttctt gagctctcca agtaaggaaa gcgccacttt
     7381 cacgtttatc aaaatggatg atgttataat aatgttgtga gcttcttgca gaatctctcc
     7441 aagctcctaa aatgggtgaa atccgaaacg atacatctac cttgtttcct aaagtttttt
     7501 ggacttttac ataattcatc aaggctgaaa aaccttgaga agtatcatca ttatcgttca
     7561 ctacattggc agcttttcgg atatacggat tattcaaagg aaaacgtatc ggacgccgca
     7621 ccattccatt ttcctgattc tttttgaatt tgtataattt cccattaggt ggaagcaaag
     7681 gccaaaaatt aaagccattt ccactgtaaa aatccatcat ccttattatt gccttgtcga
     7741 gcataggcgg tatggatgtc aaatcgggac gaatcaaaaa tgctgcggaa agagaattct
     7801 gaatggaggc attcgaaaaa caattggaat cgtatgacct ttcttgttta ccaagcatta
     7861 caaacgaaga acgcatcgtc ataaatgctg gccattctcc ttcatactga tgctctggta
     7921 tcgttttctt gacttgctcc tgttctataa acctcaatgc tccttgaata accatctcaa
     7981 tactatcagt tgaaattggt tttatctgtt gagctccaag tggcaaaact aaaaagagac
     8041 taaatagcaa taatatgcgc aatgacatgc ttcaaagata atcaagagcc acacttattc
     8101 tgaatgctaa ttagcgcaga atctcaaaac gataagcatc caaccggacg aaaataaaag
     8161 tcaaaacagt aaaggaccaa agggatgatc c
//
LOCUS       DAOOQW010000096         8168 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_10852_length_8168_cov_4.493406, whole genome shotgun sequence.
ACCESSION   DAOOQW010000096 DAOOQW010000000
VERSION     DAOOQW010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 8168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8168
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10852_length_8168_cov_4.493406"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /locus_tag="PK281_06750"
     CDS             <1..711
                     /locus_tag="PK281_06750"
                     /inference="COORDINATES: protein motif:HMM:NF018534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HOY49082.1"
                     /translation="YVVGVWVGNASGEGKPLLTGVGAAAPVLFDVLALLPHSGWFERP
                     YDDEKQVVTCRESGFIAGPFCTLPDTIWVPKSCLHTSVCPYHQRVHLNSTGQFRADAG
                     CLEADQLQARNWFVLPPVMEYYYRNSHPEYVTLPTWMPGCGIEQQQSRMEFIYPRINS
                     EIKVPRSFDGLPTRVVMEIAHRDAASTVYWHLDGKFLGQTRQIHQMAVQSELGEHLLV
                     AVDEHGEQIQRRFKLIKN"
     gene            752..1183
                     /locus_tag="PK281_06755"
     CDS             752..1183
                     /locus_tag="PK281_06755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49083.1"
                     /translation="MDILNTVHNLLRWIIIPVMLFVLIRSYMGWMGKKPFAKLDNALG
                     GALIGLAHSQLLIGLVLYFTSPRGYALFSTPGVMKDAYSRLLALEHPLMMIIAVVLIQ
                     LGRTFSKKAADDEKRFKTVAIYTTIALVLILSRQIHWNFIF"
     gene            complement(1185..>1517)
                     /locus_tag="PK281_06760"
     CDS             complement(1185..>1517)
                     /locus_tag="PK281_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49084.1"
                     /translation="DIAWSGGAGVGVMARLSSTVYLNLGANYMLGGKLDYFDKEQISQ
                     WNIELNSSALTSTNNEHQLGSDDVNIQALPKHSVTTMLFANAGICFLLNGSSSSSGPK
                     KRPGPKKR"
     assembly_gap    1518..1617
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1618..1931)
                     /locus_tag="PK281_06765"
     CDS             complement(<1618..1931)
                     /locus_tag="PK281_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49085.1"
                     /translation="MLRYISMLIFGLTFHTVCFAQVGSKMMTRKKGPSASMQNFLDLT
                     LTMGVPMDDFASTTTSLPFGLSCNYLFQPSSKVPFLFGGGLTYLSAGSKDIKKDLTAD
                     ITL"
     gene            complement(2008..2817)
                     /locus_tag="PK281_06770"
     CDS             complement(2008..2817)
                     /locus_tag="PK281_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49086.1"
                     /translation="MSKFYLISLLIVLICPTIQAQVQNSEKSRISDFCFSVGFPRNEF
                     DRSSSLQPIGLTYNFLFKNPQTSPFIPGFGFSYYQTGRLKANKNINPSIYNESAFLYS
                     QPILLKLSSVNQLFSVHLMARIQGEQSHLKPFVDLIAGMQYAWTQVTLNENEIGKYFQ
                     TDSKSRAYYETHTSDLSWIAGGSFGVLWKLNSNLYLNSSLAFLFGGKLTYVDKTSAES
                     WNVTLNNSDVQLTKFIEKFYESDLNVTAPVKTSLSNMLVAQIGLTANITKK"
     gene            3012..3093
                     /locus_tag="PK281_06775"
     tRNA            3012..3093
                     /locus_tag="PK281_06775"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3046..3048,aa:Leu,seq:tag)
     gene            3142..4506
                     /gene="tig"
                     /locus_tag="PK281_06780"
     CDS             3142..4506
                     /gene="tig"
                     /locus_tag="PK281_06780"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HOY49087.1"
                     /translation="MNVTQENVDALNAVVRIQFSPADYQPKIDEQLRDYAKKVQMPGF
                     RPGKVPAGMVKKMYGKSILVDELNKLTSDTLFNYLRDQQLDVLGNPLPKAENDSAIDL
                     DNPGEINLSFEVGLAPKFDLDLSANHRFNHYKVKLDDSYVNDIIADYRKRLGELEETS
                     VSAEGDQIHGVAVELDSDGNEKEGGLHKHITLQFEDLKEGNQQALFTGLSKDSQITID
                     FQTAVVNPTVIGAILAISKEEAEALTSTFRFTVEWVKRGTLAEVNQDFFDKLLGPGVI
                     SSEEELRARIKEDGEARYRKDADNRLFNEAVEHLVNNTRFELPNDFLKRWLVSQNEGK
                     VNPSDIESNYENYSKGIRWQLIEGKIIKDHNITVSSDQVLDSFTEDFLAYMGAPGTQD
                     ETLRGRAREIAQGMMKNEKEVNKVYDRLYNEAMTTLFLSSFDVHSVELPFEAWVEQLN
                     KPLN"
     gene            complement(4585..5391)
                     /locus_tag="PK281_06785"
     CDS             complement(4585..5391)
                     /locus_tag="PK281_06785"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOY49088.1"
                     /translation="MYRFLIVFVILLSSCGGNQEPLVRTSMRHYYEPYRINGFLALQD
                     PLRETNLISDREVFRGKYTPASTFKICNSLIGLETGVLKDEKTVFEWDSVMRDNPDWN
                     HSQNMAEAFQHSTVWYYQELARKIGGKQMKYWLDKAAYGNADTTGGIDSFWLRGGLRI
                     SPEEQLNFVKKLHNDELPFSKRSMDIVKKVMITKDSADVVFHGKSGWGHQDRMDVGWF
                     VGYAETPERVVYFCNLVRMADTSSNQENFNKSRREVVYQAVYDYLKGNLK"
     gene            5544..5912
                     /locus_tag="PK281_06790"
     CDS             5544..5912
                     /locus_tag="PK281_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020081384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2237 domain-containing protein"
                     /protein_id="HOY49089.1"
                     /translation="MQLNVLGGELAPCCLDPITGFYRDGHCHTGDEDHGSHTVCAKVD
                     AVFLDFSKRTGNDLSTPRPEFDFPGLKPGDSWCVCAARWLQAQEFGTACPIILSSTHE
                     RALELIPLEILMEHAADLLS"
     gene            complement(5914..6783)
                     /locus_tag="PK281_06795"
     CDS             complement(5914..6783)
                     /locus_tag="PK281_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49090.1"
                     /translation="MRISLLLFASFLFNQIAFSQDEPVVQDSVYLKNGNIITGRILNP
                     EAEGNIQVRTSGNSVLYISESQIVRMAVKLPPESRSPVKSDKPHQKTLIDDQFNFSIH
                     GGLGIPGGEFGGNSGSYPGFAKPGGMFQASAWVRTKNQWFWSTQFAYAQNSFNRDEFE
                     LYEEKNLNVDVFQINTGSWKAIHINTGLTWTTELNVDFQLYLQAQIGLIRVQTPEVRF
                     FATNGYLYTIDIVNGNGFTNSLAAGLVYKNRYSASLTMLSSKLYIPYPTLTVYQPYRI
                     FGFQLGYFLLRLS"
     gene            complement(6825..7679)
                     /locus_tag="PK281_06800"
     CDS             complement(6825..7679)
                     /locus_tag="PK281_06800"
                     /inference="COORDINATES: protein motif:HMM:NF021454.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2167 domain-containing protein"
                     /protein_id="HOY49091.1"
                     /translation="MIFTPVLAQENVDSTIHSEGFQEVSKEIESNYTFQQGQINLIED
                     MVSIHVPAHFHFLDSKQTKDLLINYWGEAEENAHHLGLISSRNLSVFAPEAVVFTIDY
                     VKAGHILEDRDEVFMDEDFLNHFKNSIDSSNKKRKEAGLEAVSLKGWALKPKFDTQSH
                     ALYWIEDIALGQNSESRLSANLCILGRSGYIRFSSVCVKEVLEKLKNDLTILATSLRF
                     EPEHQYSAFKPAQEKASQLTLEALILGYNYPKSSWANLLSKYWKLASFLILIFGFIFL
                     NMYPKKSI"
     gene            complement(7833..>8168)
                     /locus_tag="PK281_06805"
     CDS             complement(7833..>8168)
                     /locus_tag="PK281_06805"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002672390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HOY49092.1"
                     /translation="PYSADGFFGSGTWKKFDAVMAMYHDQGLVPFKSIAFHHGVNFTA
                     GLPVIRTSPDHGTGYDIAGKDLASPDSFREAIYTAIDIFRNRKQYQEMSSNPLQVMQR
                     RERERDRHA"
BASE COUNT         2481 a         1539 c         1579 g         2469 t
ORIGIN      
        1 tatgttgttg gcgtttgggt tggaaatgca agtggagaag gaaagccatt acttacaggc
       61 gttggtgcag cagcgcctgt tctgtttgat gttttagctt tattgccaca ttcgggttgg
      121 tttgaacgac cttatgatga tgaaaaacag gtagttactt gtcgggaatc gggatttatt
      181 gcaggtcctt tttgcactct gcccgacacg atttgggtgc ctaaatcgtg tttgcatact
      241 tctgtttgtc cctatcacca acgagttcac ttaaattcga caggtcagtt tcgagctgat
      301 gccggttgtt tagaagcaga tcaattgcaa gcgcgaaatt ggtttgtatt gcctccggtg
      361 atggagtatt attatcggaa ttcacatcca gaatatgtga cacttcctac ttggatgccc
      421 ggttgcggga tagaacaaca gcaaagtaga atggagttta tctatcctcg gataaattca
      481 gaaataaaag ttccccgatc atttgatggt ttgccaaccc gtgtggtaat ggagatagct
      541 caccgagatg cagcgtcgac agtatattgg catttggatg gaaaatttct cggtcaaacc
      601 cgtcagattc atcaaatggc tgttcagtca gaactgggcg aacacctttt agtggctgtg
      661 gatgaacacg gtgaacagat tcaacgccga tttaagctaa tcaaaaactg aagagaagag
      721 ctttcgtgta tttttggaaa aaaaatttcg gatggatatc ttaaatacag ttcacaacct
      781 acttcgttgg attatcatac cagtcatgtt attcgtgctt attcgcagct acatgggatg
      841 gatggggaag aagccttttg ccaaattaga taatgcactt ggcggagctt taataggttt
      901 agcacattct caattgttga ttggattggt gctgtatttt accagtcctc gcggttatgc
      961 acttttttca actcccggag tgatgaaaga tgcgtattct cgtttattag ctttggaaca
     1021 tcccttaatg atgattattg ccgttgtgct tattcaattg ggacgcacct tttctaagaa
     1081 agctgcagat gatgaaaaac gatttaagac tgttgcgatc tatacgacaa tcgcattggt
     1141 attaattctt tcccgccaaa tccattggaa ttttattttc tgatttagcg ttttttcggt
     1201 cctggtcttt tcttgggacc tgatgagctt gaacttccat tgagtaagaa gcaaataccc
     1261 gcattggcga acaacatggt tgtaaccgaa tgtttaggta aggcttgaat attcacatca
     1321 tcggagccta actgatgttc attgttagtt gaggtaagag cagaagaatt caactcaata
     1381 ttccattgac taatctgctc tttgtcaaaa taatcgagct tcccacctaa catatagtta
     1441 gcgcctagat tcaagtaaac agtactgctt aatcgcgcca ttactccaac acctgcgcct
     1501 ccgctccaag cgatatcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnagt
     1621 gtaatatcgg cagttaggtc tttcttaatg tctttgctac cagcggataa atacgttaat
     1681 cctccaccaa aaaggaaagg aactttacta gatggttgaa aaaggtaatt acatgaaagt
     1741 ccgaatggaa gtgaagtggt agtagatgca aaatcatcca tagggacgcc catggtaagt
     1801 gttaaatcta ggaaattttg catgctagca gaaggacctt ttttgcgggt catcatttta
     1861 gatccgactt gcgcaaaaca caccgtatga aatgttaaac caaagatgag catcgaaatg
     1921 taacggagca tcaggagaaa tttaggtttt aaaagggcaa aataattaaa aaggaatgtc
     1981 aattcgggtt tagcagaatc aattgattta tttcttggtg atgttggctg ttagacctat
     2041 ttgagcaact aacatattac ttaaactggt ttttactggt gcggttacat ttaagtcaga
     2101 ttcataaaac ttttcaatga attttgttag ttgaacatct gaattattga gcgttacgtt
     2161 ccagctttct gcagatgttt tatctacgta tgtgagtttg ccaccaaata agaaagctaa
     2221 actagagttg aggtacagat ttgaattcaa tttccaaagc acaccaaagc ttcctcccgc
     2281 gatccaagat aaatcggaag tatgtgtttc ataataggct ctacttttcg aatccgtttg
     2341 aaagtactta ccaatttcat tttcgtttaa tgttacttga gtccatgcat attgcattcc
     2401 agcaataaga tctacaaagg gctttaagtg cgattgttca ccttggattc gagccataag
     2461 gtgtacgcta aacaattggt taacgctgct tagttttaac aggataggct gagagtataa
     2521 gaatgccgat tcattataga tagatggatt gatgtttttg ttcgccttta aacgccctgt
     2581 ttgataatat gaaaatccaa atccgggaat gaaaggactt gtttgaggat tcttaaacag
     2641 gaaattgtag gtaagtccga taggttgcaa actacttgag cggtcaaatt cattccgagg
     2701 aaatccaaca ctaaagcaaa aatcagagat tcgacttttt tcagaattct gtacctgagc
     2761 ttgaattgtc gggcagatta gtacgataag taagctaata aggtagaatt tagacatagg
     2821 tcattttaag aggataaagc aacgaaatat acacttagag gacatcttcc agaggatgat
     2881 ttttgagcat ttataacaca attcgaaatg caagtatttg gtcttcacgg ctatattata
     2941 aaacctcaaa ttttatttgg ttgtaataca aaacctccct acttttgcac tcctttttaa
     3001 ttaagatcca tgcggatgtg gcgtaattgg tagccgcacc agacttagga tctggtgccg
     3061 agaggcgtgg gggttcgagt cccttcatcc gcacattgct tctccttcgg gagaagcatt
     3121 ttttattgta aagaagttcc aatgaacgta acacaagaga atgttgacgc gctcaacgca
     3181 gtagtccgga tccaattttc accggctgat taccaaccca aaatagacga gcaactccgc
     3241 gattacgcta aaaaagtaca aatgccagga tttcgtccgg gtaaagtacc tgcgggcatg
     3301 gtaaagaaga tgtatggtaa atccatttta gtggatgaac tcaacaaact aacatccgat
     3361 accttattca attacctacg cgatcagcaa ttagatgtat taggtaatcc gcttccaaaa
     3421 gctgaaaacg attctgctat cgacttggat aatccaggtg agatcaattt atcgtttgaa
     3481 gtaggtttag ctccaaaatt cgatttagat ctttcagcaa atcatcgttt caatcattac
     3541 aaagtaaaat tggatgattc atacgtgaac gatatcattg ctgattaccg taagcgtcta
     3601 ggtgaattag aagaaacgtc tgtttcggca gaaggagatc aaattcatgg cgttgctgtt
     3661 gaattagatt cagatggcaa cgaaaaagaa ggtggtttgc acaaacacat cacacttcag
     3721 tttgaagatt tgaaagaagg taatcaacaa gccttgttta caggtctgag taaagattcc
     3781 caaatcacga ttgacttcca aacagctgtt gttaatccaa ccgttatcgg tgccatttta
     3841 gcaatcagta aagaagaagc tgaagcatta actagcacat tccgttttac tgttgaatgg
     3901 gtaaagcgtg gaactcttgc agaagtaaat caggatttct tcgataaact tctaggtcca
     3961 ggtgtaatca gcagcgaaga agaacttcgt gctcgcatta aagaagatgg agaagctcgt
     4021 tatcgtaaag atgctgataa ccgtttgttt aacgaagcag ttgagcactt agtaaataat
     4081 actcgttttg aattacctaa cgactttttg aaacgttggt tagtttctca aaatgaaggg
     4141 aaagtaaatc cttctgatat tgaatcaaac tacgaaaact attcaaaagg aattcgttgg
     4201 cagttgattg aaggaaaaat catcaaagac cacaacatca cagtaagcag cgatcaagtt
     4261 ttggattctt tcacagaaga tttccttgct tatatgggag ctcctggaac acaagacgaa
     4321 acattacgtg gtcgtgctcg tgagattgct caaggcatga tgaaaaatga aaaagaagtg
     4381 aataaggtgt acgaccgact ttataatgaa gccatgacaa ctttatttct cagcagcttt
     4441 gatgttcatt cagttgagct tccttttgaa gcttgggtgg aacaattgaa caagccgttg
     4501 aattgatttt tgattgagtg tgtgaaacgg cgtcagggga aacttgggcg ccgtttttat
     4561 tttataccat ttctgtattt gctattattt caaattaccc tttaggtaat cgtaaactgc
     4621 ctgatacacc acctctcgtc tagacttatt aaaattctcc tgattactac tggtatcggc
     4681 cattctaaca agattacaga agtaaactac acgttcgggc gtttcggcat agccaacaaa
     4741 ccagccaaca tccattcggt cttgatgtcc ccaacctgat ttgccatgga aaaccacatc
     4801 ggcagaatct tttgtaatca tcaccttttt aacgatatcc atcgaacgtt tagaaaatgg
     4861 taactcatcg ttatgtagtt ttttaacgaa gttcaattgt tcttccggac taatacgcaa
     4921 tccacccctt aaccagaatg aatcaattcc tcctgttgta tcagcatttc cataagctgc
     4981 tttatcaagc caatacttca tttgtttacc accaattttc ctagccaatt cctgataata
     5041 ccaaacagtt gaatgctgaa atgcttcagc catgttttgt gaatgattcc aatcgggatt
     5101 atcacgcata acgctgtccc attcaaaaac agttttctca tcctttagta cacctgtttc
     5161 cagtccaatt aatgaattgc aaattttgaa agtggatgca ggagtatact ttcctctaaa
     5221 tacttctcga tcagaaatca aattggtttc tcttaaagga tcttgcaagg ctaagaatcc
     5281 atttattcga tatggttcat aataatggcg catggacgtt cttaccaaag gctcttgatt
     5341 tccgccgcag gaactcaata aaataacaaa cacaattaga aatcggtaca taatcaagaa
     5401 gaaatcaatc cgttggaacg tgttcgaaat tacatggatt gaaggaataa actcgcgtta
     5461 ttgctcataa gattttgcgc atttatcaaa ataacctgct ttatttgttc caatataaat
     5521 cgtacacata tcctaacaga atcatgcaat taaatgttct tgggggagaa ttagctcctt
     5581 gttgtttgga tccgataact ggattttatc gggatggaca ttgtcataca ggcgatgagg
     5641 atcatggttc tcacaccgtt tgtgcaaaag tggatgctgt atttttagat ttctctaaac
     5701 gcaccggcaa cgatttgagc acacctcggc cggaattcga tttccctgga ttgaaaccgg
     5761 gtgattcttg gtgtgtttgt gcagctcgtt ggcttcaagc ccaagagttt ggaaccgctt
     5821 gtccgattat actttcatct actcacgaac gcgcattaga actgattcca cttgaaattc
     5881 tcatggaaca tgcagctgat ttgttgagtt aaattaagac aatcgcaaca aaaagtaccc
     5941 aagctgaaat ccaaaaatgc gataaggttg atataccgtt aaggttggat aaggaatata
     6001 caatttgctt gaaagcatcg tcaagcttgc tgaatagcga tttttataaa ctaaacctgc
     6061 tgccaatgaa ttggtaaatc catttccatt tacaatatca attgtataaa gatatccgtt
     6121 ggtagcaaag aaacgtactt cgggtgtttg aacacgaatt agccctattt gtgcttgaag
     6181 gtaaagttgg aaatcgacgt ttagttcggt agtccatgtt aaaccggtat tgatatgaat
     6241 agctttccac gaacccgtat tgatttggaa aacatccaca tttaagttct tttcttcata
     6301 taactcaaat tcatcccgat tgaatgaatt ttgagcataa gcaaactgag tactccaaaa
     6361 ccattggttc ttcgttcgaa cccatgcaga tgcctgaaac attcctcctg gctttgcaaa
     6421 acctggatat gaacccgaat tgcctccaaa ttctcctccc ggaattccta aacctccatg
     6481 aatagaaaag ttgaattgat catcaattag cgttttctgg tgaggtttat cacttttaac
     6541 aggacttcga gattcaggcg gcagtttaac agccattcga acaatttgag attcagaaat
     6601 ataaagtaca gaatttccag atgttcgaac ttgaatgttt ccttctgctt ccggatttag
     6661 aatacgacca gttataatgt tcccattttt cagatagaca gaatcttgaa cgaccggttc
     6721 atcttgcgaa aatgcaattt gattaaataa aaaacttgca aataggagga gggatattct
     6781 cacgactgca agatacatcc attctaacaa tatggataat gaggctaaat tgatttttta
     6841 ggatacatat tcaggaaaat gaacccgaaa attagaatca aaaaactagc aagtttccaa
     6901 tatttagata acaaatttgc ccaagagctt ttaggataat tgtaccctaa aatcaacgct
     6961 tcaagcgtta attgcgatgc tttttcttgt gccggtttaa aagcactata ttgatgttca
     7021 ggctcaaatc gtaaagaagt tgctaagatt gttaggtcat tctttagttt ctctaatact
     7081 tccttaacac aaaccgacga aaatcgaata taacctgaac ggccaagaat gcacaaattg
     7141 gcacttaatc tcgattcgga attttgtcct agtgcaatat cttcaatcca atataaagca
     7201 tgtgattgag tatcgaattt gggttttagt gcccaacctt taagactaac agcttccaat
     7261 ccagcttctt tacgcttctt gttagaactg tcgatcgaat tcttaaaatg atttaagaaa
     7321 tcttcatcca taaacacttc gtcgcgatct tcaaggatat gtcctgcctt tacataatca
     7381 atagtaaata ctacagcctc aggagcaaat acagataaat ttctgctaga aatcaatcct
     7441 aaatgatgag cattttcttc tgcttcaccc caatagttaa tcaataaatc tttggtttgt
     7501 tttgagtcaa gaaagtgaaa gtgtgcaggg acatgaatac taaccatgtc ttcaatcaag
     7561 tttatctgac cttgttgaaa agtgtagttt gattcaatct ctttacttac ttcttgaaaa
     7621 ccttcggaat gtattgttga atcaacattt tcctgtgcta aaactggagt aaatatcaaa
     7681 ctacaaaata acactcttac aagtgagaaa ctgctcttta ctgaactaat cattttaata
     7741 cccctttaat tcaagcaaga ttacctgaaa attaaatagg cttgttgaat tgtcctaaat
     7801 atactccaaa aggatgagag aaacgagcat ttttaagcgt gacgatctcg ttcgcgttcc
     7861 cttctctgca tgacctgaag aggattagaa ctcatttcct gatactgttt gcgattccgg
     7921 aaaatatcga tagctgtata aatagcttct cggaatgaat caggactagc tagatctttt
     7981 ccggcaatgt cgtaacctgt tccatgatcg ggtgatgtac gaattacagg taaaccagcc
     8041 gtaaaattca ccccatgatg aaaggcaatc gatttgaatg gtacaagtcc ttgatcatga
     8101 tacatagcca tcaccgcatc aaactttttc cacgtcccac ttccaaaaaa tccatcagcc
     8161 gaataagg
//
LOCUS       DAOOQW010000097         8068 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_11034_length_8068_cov_4.870086, whole genome shotgun sequence.
ACCESSION   DAOOQW010000097 DAOOQW010000000
VERSION     DAOOQW010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 8068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8068
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11034_length_8068_cov_4.870086"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(27..1469)
                     /locus_tag="PK281_06810"
     CDS             complement(27..1469)
                     /locus_tag="PK281_06810"
                     /inference="COORDINATES: protein motif:HMM:NF014049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF92 domain-containing protein"
                     /protein_id="HOY49093.1"
                     /translation="MHKDSVILVFSGLLVLFISITLEVLSRKRILPQWIGRKLLHISA
                     VSTCALVPLYLEDLMLLQWIVVIAELFLIYLVWKGFLFEEEHKRKSWGIALFPLPFIA
                     FLFLFEEQRWLISFPMLILAWCDAVAAIVGIALAKKYFFMSGDPKSLAGSLAFFISSV
                     FLFLLVRFGFFPLFPNWIDVPFTSFILVAALFSIILSAVEALGSQGSDNLFIPIAALL
                     LFLRMDEILAQIHAFSILVLLAGLFIFITIRKKSLSPSGAFTAAFMGISVAEFAGAEW
                     LILPAAFFLSSTLIGRIFKGKTSHGDAKQGKARDAVQVVCNGGVYWVMALLLPYFPEL
                     SLAMIVSMAIATSDTWASEIGIGLKGKTYDIIRWKSLPVGVSGGVSLGGSLAGLFGSV
                     LIVFISIYLTELSIFTCILLISLGFIGMLLDSIIGSVFQAKYLSSEGLSDENTGQQSQ
                     LISGYSWMSNDWVNLISNILITTPVQDVLK"
     gene            1551..2255
                     /locus_tag="PK281_06815"
     CDS             1551..2255
                     /locus_tag="PK281_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49094.1"
                     /translation="MQTAYIGWLVAQFGVYKPIQEKFADAATLSQATCWTDLASGNGG
                     PALSLLSELKKSNKKWEQFPLELTDLYPGFPTKLPHHVITNNSAIDAITWQSQQKDIR
                     TLFNAYHHFDANQKAQLIQQHGKKGFFVAEVLQPNVLVLIKIIFTTTIGQILLATFVK
                     PFSWTRLLFTYIFPINLLTITWDGIVSVLKSESHTKMSAHAKQHLPAGCLIKSGIHGP
                     WWAPVSWFYIVPANHE"
     gene            2248..3138
                     /locus_tag="PK281_06820"
     CDS             2248..3138
                     /locus_tag="PK281_06820"
                     /EC_number="2.5.1.115"
                     /inference="COORDINATES: protein motif:HMM:NF009525.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate phytyltransferase"
                     /protein_id="HOY49095.1"
                     /translation="MNKLKILWQFSRPHTIIGSTLSILALYIIALNGSAFSIEQLVIV
                     GISLISAWGCNIYITGLNQWSDVEVDKVNKPWLPIASGELSRPAALNIVLVCGGISLI
                     SAAYLSLPFLGLIASIMLIGTAYSLPPLKLKRSHLAAASAIALVRGVLINLGFYWHFQ
                     HAFLLQNEFNPFIGSLIFFVVAFSLGIAWFKDIPDTRGDAEYSFQTLAVKTGRTGAFR
                     AGLFVVSLAYLSVIILAAISQIPAPIFMISSHAILWGIFLFMAYRLDLTNDTSIKQFY
                     MLFWGLFFVEYVVYALATCI"
     gene            complement(3135..4010)
                     /locus_tag="PK281_06825"
     CDS             complement(3135..4010)
                     /locus_tag="PK281_06825"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HOY49096.1"
                     /translation="MTTHQRGKIKLLDYWGFIGIHFVPLLAIFTGTFAFDWYLALALY
                     LIRMFFITAGYHRYFSHRTFKTSRVFQFILAYMAQTSMQKGALWWAANHRIHHKYSDT
                     PADPHSAKLFGFWYAHVGWIVGPDFKETRYDLIKDMAKYPELVWLNKYHWVPAASLMV
                     FCLFLGNYVNGAGILDFQAGVSTLLIGFFLSTAVLYHGTFTINSLMHEMGFPRYKTTD
                     ESKNSVILALVTLGEGWHNNHHYYQSTARQGFYWWEIDITYYIIRLLGFFRIVWDIRE
                     VPKHVKDGNHLKSAA"
     gene            complement(4507..6612)
                     /locus_tag="PK281_06830"
     CDS             complement(4507..6612)
                     /locus_tag="PK281_06830"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HOY49097.1"
                     /translation="MIFRIIIVFLSLFLSLSASSQSLEKGFAALKQYDFFLAKKVFTA
                     KLKKNPVPAHFGLAQVRYDRLNHFHSLDSAYVNMLKADSAWKLLSPSKKIDLLEYGIS
                     DTAISGLTNRILEAAFVRAKQQNTPSSYAYFRTFFKQSTRLDEAYKLEMARAFFLADS
                     LGKSDDLKAFLSNYPEAIQKEQALRRLDDVLFLEETKEQSRDQFAAFISKYPSSAHKG
                     EAEDALYALMAPQRSRETLYEFVKAFPQNRNADEAWDLIYADFTSDQRVESFEAFKKA
                     YPEYPYPDRLNRDFNLAGIKLYPAVQGELWGFVDSTGKVVLSYQYEEVWSFSENLAMV
                     RLDGKVGYINKAGIRVVQPIYNDGEPFHNQVAIVEKDGLYGIIDQRGKVVLPIEYEIV
                     DGPDNGFYIISKGEFYGFADAKGKIIQEPVYENMDPFSEGLAAVTKEGLTGFVDTTGK
                     LVIPFLYEDVILFENGRVRVMKNDRVGLIDQTGKEIISLKNDRIGKFQEGLARVSRDG
                     KCAFYDINGKEIIPFQPTCSSPILGIDGFSEGLARVEKKGKKGYINRKGKLVIPMEWE
                     ESGYFIWGRAPFKKKNRWGYLTTQGKVAVEAQFDEAYPFVDSRARVKKKGKFGLIATD
                     GTVLLPFEWDELQEDAGWYIGVKNGKQVLFDSQLKPQSEVTWDEIRRTSDKEVFQFTA
                     GSKMVLYHTGMKKVFWEEH"
     gene            complement(6670..>8068)
                     /locus_tag="PK281_06835"
     CDS             complement(6670..>8068)
                     /locus_tag="PK281_06835"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY49098.1"
                     /translation="FGICDVDITENQYIINTHGEYYLSISKYSPDIELIWNRTFVSSN
                     SSLQYGGLLAVDNNDNILISGSYRGELYATDSLVNILSNVNYTTDFILKLDSSGNLVW
                     ANKFIVNFGMNTIHTIEMANSELYLGGMFSSSADFDMSSNNYIQDSYGGFDVFIAKYS
                     PCFNTFLQYNIVDCNSYHLPSGREVFFSGSYQDTIPNHLGCDSILTINLTLLKSYSSP
                     NIQECSSYLAPDGTILNHSGSYDFVIQNSMGCDSIISLHLTILNSSSINLVNTCHSYT
                     APDSTIYYTSGLISSIIPNSFGCDSVIYTNLVIHNLNTAILVTDSNLTSLSVNSQYQW
                     ALCTNVFEIIPNAINSTFFPSENGDYALILSQDGCVDTTMCYSFVLSGIEDIYSQNIG
                     IFPNPGHEHFDILLQDKSNITELKLYDMQGRQINNHWIVNDSLSSFDFNGNPGFYILQ
                     VNFLHSSQYVKFIKL"
BASE COUNT         2666 a         1645 c         1357 g         2400 t
ORIGIN      
        1 atccggacca actctatttt tataccctat tttaaaacgt cttggacggg tgtggtaatt
       61 aatatattac ttattaagtt aacccaatcg ttcgacatcc aagaataacc actaattagt
      121 tgagattgct gtcccgtatt ttcatctgaa agtccttctg aactaaggta tttggcttga
      181 aacacagaac caataatact atctaataac attcctatga aacctaaact aatcaataaa
      241 atacacgtga aaatggataa ttcggttaaa taaatactaa taaatacaat caatacagag
      301 ccaaataaac cagctaaact tcctccaaga gaaacaccgc ccgacacgcc aactggcaat
      361 gatttccatc gaattatatc ataggttttc ccttttaaac caatacctat ttcagaagcc
      421 caagtatcgg atgtagcaat agccatcgac actatcatgg ctaaactcaa ttccgggaaa
      481 taaggaagca gtaaagccat cacccaataa acacctccat tacaaactac ctgaacagca
      541 tcacgcgctt tgccttgctt agcatctcca tgtgatgtct tccctttgaa aattcgacca
      601 atgagcgtac tacttaagaa aaaagcagca ggcaaaatca accattcagc accagcaaat
      661 tcagctactg aaattcccat gaatgcagct gtaaaagcac cactcggact caaggatttt
      721 tttcgaatgg taataaatat gaataatcca gcaagtaaga caagaatcga aaaagcgtga
      781 atctgcgcga gaatttcatc catgcgcaaa aacaataata aagctgcaat gggaataaat
      841 aaattatcgg aaccttgaga accaagcgct tcaacagcag acagaataat agaaaataaa
      901 gcagcaacta gtataaagct ggtaaatgga acatcaatcc aattaggaaa aagtggaaaa
      961 aatccgaaac gaactaatag aaataaaaat acactcgaaa taaaaaatgc caaactacct
     1021 gccagtgatt tgggatcacc actcataaag aagtactttt tagctaatgc aattccaaca
     1081 atagcagcta ctgcatcaca ccaggcaagt atcaacatgg gaaaagaaat caaccaacgt
     1141 tgttcctcaa acaaaaatag gaaagctatg aatggtaaag gaaacaaggc aatgccccaa
     1201 cttttacgtt tatgttcttc ttcaaataaa aatcctttcc acactaaata aatcaagaat
     1261 aattcggcga tgacaactat ccattgaaga agcattaaat cttccagata caaaggcacc
     1321 aatgcacaag tgctgacagc cgatatgtgc agcagtttcc gcccaatcca ttggggtaat
     1381 attcgtttac gagacagcac ttcgagtgtt atggaaataa ataacaccag taatcccgaa
     1441 aaaactaaaa tgactgaatc cttatgcata ggtttgtgta agataaccca tagttgctca
     1501 ggatgtttga atgggaggat aaatcgtggt ttccaaaagt atttcgagac atgcaaacag
     1561 cttacatcgg ctggctggtg gctcaatttg gtgtgtataa accaatccaa gaaaaattcg
     1621 ctgatgcggc aactttgtcc caagcaacct gttggaccga tctagcatca ggcaatggtg
     1681 gaccagcgct gtcgctatta agtgaactga aaaaatcgaa taaaaaatgg gagcaatttc
     1741 cattggagtt aaccgattta tatccaggtt ttccaacaaa actaccacat catgtaataa
     1801 caaataattc tgcaattgat gcgataactt ggcaatctca gcaaaaagat attcgcacac
     1861 tttttaatgc ttaccatcat ttcgatgcta atcaaaaggc tcaattaatc caacaacatg
     1921 gtaaaaaggg attttttgtg gcagaagtat tacagcccaa tgtgctggtg ttaatcaaaa
     1981 ttattttcac cactacgata ggtcaaattc tcctagctac tttcgtaaaa cccttttcct
     2041 ggacgagatt attattcact tacatatttc caataaacct tttaactatt acttgggatg
     2101 gaatagtaag tgttttaaaa tcagaaagtc atactaaaat gagtgctcat gctaaacaac
     2161 atctaccagc tggatgtttg ataaaatctg gaattcatgg gccttggtgg gcacctgtca
     2221 gttggtttta catagtgcct gcaaatcatg aataaactca aaattctttg gcaatttagt
     2281 cgcccacata ccattattgg aagcacactc agcattttag ctctctatat aattgcgtta
     2341 aatggaagtg cattttcaat tgaacaactc gtaattgtag gtattagttt aattagtgct
     2401 tggggctgca atatttatat caccggttta aatcaatggt ccgatgtgga agttgacaaa
     2461 gtcaataaac cttggttacc cattgcatcc ggcgaattaa gtcgaccagc agcgttgaat
     2521 atcgttcttg tttgtggagg gatttccttg atttcagctg cgtatttgtc gctacctttc
     2581 ttaggattaa ttgccagcat tatgctcatt gggacagcat attctcttcc tccattgaaa
     2641 ctaaaaagaa gtcatttagc tgcagcttcg gccattgcat tagttcgagg tgtgcttatc
     2701 aatttgggat tttattggca tttccaacac gcatttttac ttcaaaatga attcaatcca
     2761 tttatcggat cgctcatctt tttcgtagta gcatttagtc tcggaatagc ttggtttaaa
     2821 gatattcctg atacacgagg tgatgcagaa tattcgtttc aaacattggc tgtaaaaacc
     2881 ggacgaacag gtgcttttag agctgggttg tttgtggttt cactagcata tttgagtgta
     2941 attattctag cagctataag tcaaatccct gctcccatct tcatgatttc gagccatgca
     3001 attctatggg gaatttttct tttcatggct taccgactcg atttaacaaa cgatacatcc
     3061 atcaaacaat tctacatgct tttttggggt ttattctttg ttgaatatgt ggtatatgct
     3121 cttgctactt gtatttaagc agcgctcttc aggtgattac cgtctttgac atgtttcggt
     3181 acctctcgta tatcccatac aattctgaaa aaaccgagta gccgaatgat gtaataggtg
     3241 atatcaattt cccaccaata aaatccttgt cgtgcagtac tttgatagta atggtgattg
     3301 ttgtgccagc cttcacctaa tgttaccaaa gctagaatga cgctgttctt actctcgtcg
     3361 gttgttttgt agcgaggaaa gcccatttcg tgcatgagtg aattgatcgt gaaagttcca
     3421 tgataaagga cagccgtact caaaaagaac ccgattaata gcgtgctaac acctgcctga
     3481 aaatcgagta ttccagctcc attcacataa ttcccgagga ataaacaaaa aaccattaac
     3541 gatgcagctg gaacccaatg gtatttattg agccaaacta gttcaggata tttagccatg
     3601 tcttttatca gatcatagcg ggtttcttta aagtcgggac caacaatcca gcccacatga
     3661 gcataccaga aaccgaacaa cttcgccgaa tgtggatcgg caggagtatc gctgtatttg
     3721 tggtgaatac gatgattggc agcccaccac aatgcacctt tttgcatgct tgtttgtgcc
     3781 atgtatgcca gtatgaactg aaatacacga gatgtcttaa atgttcggtg tgaaaaatac
     3841 cggtgataac cagctgttat aaagaacatc cgaattaaat acagcgcaag tgctaaatac
     3901 caatcgaatg caaacgttcc tgtgaaaatg gctaaaagtg gaacaaagtg aattcctata
     3961 aatccccaat aatccagaag cttaatttta cctctttgat gtgtcgtcat gcgtagtttt
     4021 cctaaccaaa tctcaccgaa ttagtggtat acttaattct ttcaacctaa acgggcgcaa
     4081 acataacaca gaacatcttc gtatgttgaa tttatgcctt tatttaaaga ccccaataac
     4141 tatatatcag ataatcagca cgtttgattc tttaactcta aaagattatt cattccctaa
     4201 ttagacattt ctagaattga ctaaatagca aaccagcaca tcatcaaacc agcacgcttc
     4261 gacatgctca gcgcgcggtc attgtggctt agaaactccg ctccctgagc ctgtcgaagg
     4321 gagcaaacca gcatatcttc aaattttcaa ctcttcacat cttcaaattt tcaactcttc
     4381 aaattaaaac ttgtcccaca gacataacat tcagccccaa atacctcaac caaaccagca
     4441 catcagcccg cctgattggc gggcaagcaa accatcacac cagcacatca acacatcagc
     4501 acatcatcaa tgttcctccc agaatacctt cttcattcca gtatggtaca aaaccatttt
     4561 gctacctgca gtaaattgaa atacttcttt atcgctggta cgacggattt catcccaagt
     4621 aacttccgat tggggtttta gctgcgaatc aaataaaacc tgcttgccat ttttcacacc
     4681 tatataccaa ccagcatctt cttgtaattc atcccattcg aatggtaata acacagttcc
     4741 atcggttgca ataagtccga atttaccttt cttcttcact cgggctcgac tatctacaaa
     4801 aggatatgcc tcatcaaatt gagcttcaac agcgactttg ccttgggtag ttaaatagcc
     4861 ccagcgattc ttctttttaa atggagctct tccccaaata aaatatccgc tttcctccca
     4921 ctccattggg attaccaatt ttcctttccg gttgatgtag cccttttttc ctttcttttc
     4981 cactcgggcc aatccttcac taaagccatc tatgcctaaa attggagaag aacaggttgg
     5041 ttgaaacggt atgatttctt tcccgttgat gtcataaaat gcacatttcc catctcgact
     5101 aacacgagct aatccttctt gaaacttacc tattcgatca tttttgagtg agatgatttc
     5161 tttcccggtt tgatcgatta atccaacacg atcgtttttc attactcgaa cgcgaccatt
     5221 ttcaaacaat atcacatcct catacaaaaa tggaataacc aattttccag tagtatcaac
     5281 aaatccggtc agtccttctt ttgtcacggc agctaatcct tccgaaaacg gatccatgtt
     5341 ttcataaaca ggttcctgaa tgatttttcc tttcgcatcc gcaaaaccat aaaattctcc
     5401 tttgctgatg atgtagaaac cattgtcggg cccatcaaca atttcatact caataggtag
     5461 aacaaccttt cctctttgat caatgatacc gtataaacca tccttttcaa ctatggcaac
     5521 ttgattgtgg aatggttctc catcgttgta aataggctgt acaacacgaa ttccagcttt
     5581 gttgatatac ccaacttttc catctaatcg caccatggcg agattttccg aaaacgacca
     5641 aacttcttcg tactgataac ttagaactac tttccctgtt gagtcaacaa atccccacaa
     5701 ttctccttga acagcaggat atagtttaat tccagctaaa ttaaaatcac gatttaatcg
     5761 gtctggatag ggatattcgg gataggcttt tttgaatgct tcgaaacttt ccactcgttg
     5821 atcagaagtg aaatcagcat aaatcaaatc ccaagcttca tcggcatttc ggttttgtgg
     5881 gaatgccttg acaaattcat ataatgtttc tcggctacgt tgcggtgcca tcaatgcata
     5941 caacgcatct tcggcttctc ctttatgagc tgaagaaggg tatttagaga taaatgccgc
     6001 aaattgatca cgcgattgct ctttagtttc ttctaagaat agcacatcat cgagccgacg
     6061 taatgcttgt tccttttgaa tcgcttcagg ataattgctg agaaatgctt tcaaatcatc
     6121 cgatttcccc aaactatcag caaggaaaaa tgcacgagcc atttctagtt tataggcctc
     6181 atctaagcga gtagattgct tgaaaaatgt gcgaaaatag gcgtagctgg atggtgtatt
     6241 ttgctgtttg gcacgaacaa aagctgcttc taaaattcga ttagtcaatc cagaaattgc
     6301 agtgtctgaa ataccgtatt ccaaaagatc tatcttctta gaaggactta acaatttcca
     6361 ggcagaatca gctttgagca tattcacgta tgccgaatcg agcgaatgaa aatgattcaa
     6421 tcgatcatat cgtacttgag ctaaaccgaa atgtgcaggt acgggatttt ttttcagttt
     6481 agctgtaaat accttttttg ctaaaaagaa atcatactgt tttaatgccg cgaagccctt
     6541 ttctaatgac tgagatgaag ccgatagact aagaaaaagc gataaaaaaa ctatgataat
     6601 gcgaaatatc atgctgcaaa tatagggttt gagattatag tgcagctaat tttgaatagt
     6661 tagtgtcttc tataacttaa taaatttaac atactgactt gaatgcagga aattcacttg
     6721 caaaatataa aatcctggat ttccattaaa atcgaaggat gataaagaat cattcacaat
     6781 ccaatggttg ttaatttgac gcccctgcat gtcatacaat ttaagctcag taatatttga
     6841 cttatcctgt aatagaatat cgaaatgttc atgccccgga ttaggaaata ttccgatatt
     6901 ttgactatat atatcttcaa ttccagaaag aacaaacgaa taacacatcg ttgtatctac
     6961 gcatccatcc tgacttaata ttaaagcgta gtcaccgttt tcacttggga aaaaagtaga
     7021 attaatggca ttgggaataa tttcaaaaac attagtacat aaagcccatt gatactgaga
     7081 gttaactgaa agggaagtta aattagaatc agtaacaaga atagctgtat ttaaattatg
     7141 gattactaaa ttagtataga taacactatc acaaccgaaa gaatttggta tgattgaaga
     7201 aatcaaaccg gatgtataat agatggtact atcgggagct gtatatgaat gacaagtatt
     7261 aaccagattg atagatgacg aatttaaaat ggttaaatgg agtgaaataa tactatcaca
     7321 ccccattgaa ttttgtatga caaaatcata agaaccagaa tgatttaaaa tagtaccatc
     7381 tggagctaag taagaactac attcttgaat attagggcta ctgtatgatt ttaatagagt
     7441 gagattaatt gttaatatag aatcgcatcc taaatgatta ggaatagtat cttgataaga
     7501 tcctgaaaag aaaacctctc ttccacttgg taaatgataa gaattgcaat caacaatatt
     7561 atattggaga aatgtattaa aacagggtga atattttgca ataaaaacat caaatccacc
     7621 atatgaatct tgtatataat tatttgagct catatcaaaa tcggcagatg aactaaacat
     7681 accaccaaga tacaattctg aattagccat ttcaatagta tgtatagtgt tcatcccaaa
     7741 attaacaatg aatttatttg cccacactaa atttccagaa ctgtcaagtt ttagaatgaa
     7801 atcagttgta taatttacat tagatagaat attaactaaa ctatccgttg catataattc
     7861 ccctctataa cttccagaaa taagtatatt atcattatta tcaactgcaa gtaaaccacc
     7921 atattgtaaa gaactattag atgatacaaa tgttctgttc caaattaatt ctatatcagg
     7981 tgaatattta gaaattgata aataatactc cccatgtgta tttataatat actgattttc
     8041 tgtaatatct acatcacaaa ttccgaat
//
LOCUS       DAOOQW010000098         7998 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_11161_length_7998_cov_4.902682, whole genome shotgun sequence.
ACCESSION   DAOOQW010000098 DAOOQW010000000
VERSION     DAOOQW010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 7998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7998
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11161_length_7998_cov_4.902682"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1330
                     /locus_tag="PK281_06840"
     CDS             <1..1330
                     /locus_tag="PK281_06840"
                     /inference="COORDINATES: protein motif:HMM:NF025458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4270 family protein"
                     /protein_id="HOY49099.1"
                     /translation="KKFRLTYPEHSFWSVPGLLLFSCLFIGFNSCKQPDLGLEVQNPS
                     DAINLQKKDDFTVRTTLEREDSVKTDELSLNLLGAYNDPYLGSMRAGFYTQLRPSVSN
                     VSLGEGFIADSIVLVLPYRGFYGDITKLNGLQKFSVYRVTQDFLQSTAYYSNDSLTVN
                     TVPLGESGLIVPVLSDSVRVTGKKEAPQLRIRLDMALAQDIYNNPSSLVDGDAFVQFF
                     KGIYVRSETPTTLEGTGAILDFSLTSGARIDLFYKNNANDSLRTSFSVNESCARFSRF
                     NHTYSTLVSNLISTPALANEQSFCQTMAGLRTRIDFPDLKSWQAGRNILVNEALLIVP
                     VDESKIGKYLPNPQLNLITKGESGDLQNTLDLIIGDTWAGGTYNSTEKQYVFNISRHV
                     QGLLNNLITDRGLFIQATGTGVSSYRVPLKGGASADRPIRFEIMYQVLPQ"
     gene            1344..3191
                     /gene="glmS"
                     /locus_tag="PK281_06845"
     CDS             1344..3191
                     /gene="glmS"
                     /locus_tag="PK281_06845"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007646960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HOY49100.1"
                     /translation="MCGIVAYMGEREAYPILLKGLHRLEYRGYDSAGVALLTPGQDIR
                     LFKCKGKVSDLENFVVSKDVSGHVGVGHTRWATHGVPDDKNAHPHFSESGKLVIIHNG
                     IIENYASLKKELENRGHVFKSDTDTEVLIYLIENVLENEKTDLVTAVQIALSQVVGAY
                     AIVILSKENPDLMIGAKKGSPLVVGVGPNHEYFIASDATPIIEYTRNVVYLDDEEIVI
                     LETGKEMKIKTIRNQDKTPYVQALEMQLETIEKGGFEHFMLKEIYEQPRSIRDNMRGR
                     LNIELGEVTLGGIIDYQSKIMNAHRIIIVACGTSWHAGLVGEYLFEDWARIPVEVEYA
                     SEFRYRNPIITENDVVIAISQSGETADTLAAIELAKSKGATIFGICNVVGSSIARATH
                     AGSYTHAGPEIGVASTKAFTAQVTVLTLMALVIANKKGTITQAKYHLMLNSLDQIPAK
                     IEEMLKLDDQIRYISSIYKDANNFLYLGRSYNFPIALEGALKLKEISYIHAEGYPAAE
                     MKHGPIALIDENMPVVVIAPSRGSYEKILSNVQEVKARGGKIIAIVTQGDKDVSALAD
                     HTIEIPDVEEELTPLMSVIPLQLLAYHIAIMRGCNVDQPRNLAKSVTVE"
     gene            complement(3261..3824)
                     /gene="frr"
                     /locus_tag="PK281_06850"
     CDS             complement(3261..3824)
                     /gene="frr"
                     /locus_tag="PK281_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010523249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOY49101.1"
                     /translation="MNELVKPQIDSATQLMDKAIAHLEAELAKIRAGKASPAMLDGIY
                     VDYYGSNTPLSQVANINTPDARTLAVQPWEKQMLGPIEKAIFGSNIGLTPQNDGVMIR
                     LNIPPVTEERRLNLVKQSKGEAENAKVSIRNVRRDANEAIRKLVKDGLAEDVAKDAEV
                     KIQQLTDSYIAKTEKHLEAKEKEIMTV"
     gene            complement(3881..>4027)
                     /gene="pyrH"
                     /locus_tag="PK281_06855"
     CDS             complement(3881..>4027)
                     /gene="pyrH"
                     /locus_tag="PK281_06855"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002979821.1"
                     /note="Catalyzes the phosphorylation of UMP to UDP;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOY49102.1"
                     /translation="EKGLNVMDMTAFTLCNDNKLPIIVFDMDSSGNLKRLIDGERVGT
                     LVEF"
     assembly_gap    4028..4127
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4128..4372)
                     /gene="pyrH"
                     /locus_tag="PK281_06860"
     CDS             complement(<4128..4372)
                     /gene="pyrH"
                     /locus_tag="PK281_06860"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009414641.1"
                     /note="Catalyzes the phosphorylation of UMP to UDP;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOY49103.1"
                     /translation="MKYKRILLKLSGESLMGNKNFGIDSDRLSQYAQEIKEIQATGVE
                     VAVVIGGGNIFRGIQAAEGGMDRVQGDYMGMLATVIN"
     gene            complement(4470..5309)
                     /gene="tsf"
                     /locus_tag="PK281_06865"
     CDS             complement(4470..5309)
                     /gene="tsf"
                     /locus_tag="PK281_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HOY49104.1"
                     /translation="MSSVAITASDVNKLRQMTGAGMMDCKKALTEANGDFEAAVDFLR
                     KKGQKVAGNRADRDANEGVVIARVSADNSRAVLVVLNCETDFVAKNQDFIDFTTKISD
                     IALNTKPADVDALLAASFDGLPISERINEQVGKIGEKMELHYSQLEAPFVVAYNHPGN
                     RLATIVAFNKAGVAGIETVAKDVSMQAAAMAPVAVDKDDVDTHTLERELDIAREQIRA
                     EGKPEEMVEKIAAGKLNKFYKDNTLLNQEFVKDNKMSVRQYIQTIDKDLTVTAFKRHA
                     LGN"
     gene            complement(5334..6101)
                     /gene="rpsB"
                     /locus_tag="PK281_06870"
     CDS             complement(5334..6101)
                     /gene="rpsB"
                     /locus_tag="PK281_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015481805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOY49105.1"
                     /translation="MARISTDQLLEAGVHFGHLKSKWNPNMAPYIFMERNGIHVIDLN
                     KTVVKLEEAAAALKQVAKSGKKILFVATKKQAKEIVAESVKKVNMPYVTERWPGGMLT
                     NFATIRKAVKKMATIDKMAADGTYETISKKEKLTISREREKLETQFGSITDMSRLPAA
                     LFIVDISKEHIAVAEARRLNIPTFAIVDTNSNPNLVDYPIPANDDAAKSISLIMDVVI
                     AAVQEGLSERKQEKEKGDDEQHAKAETAAAADSTPKD"
     gene            complement(6123..6509)
                     /gene="rpsI"
                     /locus_tag="PK281_06875"
     CDS             complement(6123..6509)
                     /gene="rpsI"
                     /locus_tag="PK281_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013997327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOY49106.1"
                     /translation="METINTSGRRKTAVARVYLKPGTGKITINKRDISDYFTVPTLRG
                     KVLQPFLVTEHEGKFDVTANIQGGGITGQAGALSLAIAKALVELDAANKPALRTNGLV
                     TRDPRMVERKKPGQKKARKRFQFSKR"
     gene            complement(6509..6964)
                     /gene="rplM"
                     /locus_tag="PK281_06880"
     CDS             complement(6509..6964)
                     /gene="rplM"
                     /locus_tag="PK281_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016478129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOY49107.1"
                     /translation="MDTLSYKTVSANKATVTKEWVLVDAENMVLGRLSSQVAMLIRGK
                     KKPNYTPHVDCGDNVIIINAEKIVLSGNKTDEKVYTRYTGYPGGQRFQTPRELLNKKP
                     EAIIEKAVKGMLPKSRLGRALFGNLFVYTGSEHPHAAQQPKTININDLR"
     gene            complement(7160..>7998)
                     /locus_tag="PK281_06885"
     CDS             complement(7160..>7998)
                     /locus_tag="PK281_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl transferase"
                     /protein_id="HOY49108.1"
                     /translation="THPNDIPYLPIQFFKDQRVFNSQTSSVLEFSSSGTTGMTTSIHH
                     VADTLLYEESFTEGFIREYGSPSNSVILGLLPAYLERPGSSLVYMTEGLIRQSGRAES
                     GFYLNNLSELLDVLKRMASQNQRVWLIGVSFALLDLALLAPPVWRELTVVETGGMKGR
                     RKEMIREELHSTIRETWPVAHLHSEYGMTELFSQAWMNDSGRFHCPPWMDIHIRDAND
                     PFTYVEAGKSGGINVIDLANLDSCSFIATQDLGVKGKDGTFDVIGRFDTSDLRGCNLL
                     VS"
BASE COUNT         2233 a         1646 c         1625 g         2394 t
ORIGIN      
        1 gaaaaaattc cgccttacat acccggaaca ttccttttgg agtgttccgg gtttactgtt
       61 gtttagctgc ctttttatcg gctttaattc atgtaaacaa ccggacctcg gtcttgaagt
      121 tcaaaatcct tctgatgcaa ttaatcttca gaagaaagat gactttacag ttcgtaccac
      181 attagagcgg gaagattctg taaaaacgga tgaattatca ctcaacttgc tcggcgctta
      241 taacgatcct tacctaggtt cgatgcgtgc cggtttttat acccagcttc gaccaagtgt
      301 tagtaatgtg agtttaggcg aaggttttat cgccgattcc attgtattag ttctcccata
      361 tcgaggtttt tacggggata tcaccaaact caacggactg caaaaatttt cagtttatcg
      421 ggtaactcag gattttcttc aaagtactgc gtattacagc aacgattcac ttactgtaaa
      481 cacagttcca cttggagaga gtggattaat cgtacctgtt ttaagtgatt ccgttcgtgt
      541 tacggggaaa aaagaagctc ctcaattgag aatacgattg gatatggctt tagcccagga
      601 tatttacaat aatccttcat ccttggttga tggcgatgcg tttgtgcagt ttttcaaagg
      661 catctatgtg cgttctgaaa ctcctacaac actagaaggt accggagcaa ttcttgattt
      721 ctcacttact tcgggtgcac gtattgactt gttttataaa aataatgcga acgatagtct
      781 gcgaacttcc ttcagtgtga acgaaagctg tgctcgtttt tctcgattca atcacaccta
      841 ctccacactt gtttctaact taatttcaac tccggctctg gcaaacgaac aaagtttttg
      901 tcaaactatg gctggattaa gaactcgaat cgatttccct gatttgaaat cttggcaagc
      961 aggacgaaac attttggtaa acgaagcttt gttgattgtt ccggtggatg aatctaagat
     1021 tggaaaatac cttcccaatc ctcaattaaa tttgattact aaaggagaat cgggggattt
     1081 gcagaatacg ttagatttaa taattggaga tacttgggca ggaggaactt ataattcaac
     1141 cgaaaaacaa tatgttttca atatttcccg acatgttcag ggattattaa ataacctcat
     1201 taccgataga ggattgttta ttcaagcaac aggtaccggg gttagttcgt atcgggtacc
     1261 tttgaaaggc ggagcatctg ccgacaggcc aatacggttt gaaataatgt atcaagtttt
     1321 acctcaataa tacaacaaat actatgtgcg gaattgtagc atacatgggc gaacgggaag
     1381 cttacccgat cttgcttaaa ggacttcacc gactcgaata ccgaggatat gacagtgctg
     1441 gtgtggcatt acttacgccg ggacaagata tccgactctt taagtgcaaa ggaaaagtct
     1501 ctgatttgga gaattttgta gtgagcaaag atgtttcagg acacgttgga gttggtcata
     1561 cacgttgggc tactcatgga gttcctgacg ataaaaatgc acatcctcac ttttctgaat
     1621 caggaaagtt ggtcatcatt cacaatggta ttatcgagaa ttatgcttct ttgaagaaag
     1681 aactcgaaaa ccgtggacat gttttcaaga gtgatacgga tacggaagtg ttgatttacc
     1741 tcatcgaaaa cgtcttagaa aacgaaaaaa cagatcttgt tactgcggta caaattgctc
     1801 tttctcaagt ggtaggagca tacgctatcg tgattctatc gaaagaaaat cccgatttga
     1861 tgattggggc aaagaaaggt agtccgctag tggtgggcgt tggtccaaac cacgaatatt
     1921 ttattgcttc tgatgcaact ccaatcattg aatacacgcg taatgtggtg tacttagatg
     1981 atgaagagat tgtgattttg gaaacgggta aggagatgaa aatcaaaacc atccgcaacc
     2041 aagataaaac accttatgtt caggctttag aaatgcaatt ggagaccatc gaaaaaggtg
     2101 gattcgagca tttcatgttg aaggaaattt atgagcaacc acgctccatt cgtgataaca
     2161 tgcgaggtcg attaaacatc gaacttggtg aagttacatt gggcggtatc atcgattatc
     2221 aaagtaagat tatgaatgca caccgcatca ttattgttgc ttgtggtaca tcttggcatg
     2281 ctggattagt gggagaatat ttgttcgaag attgggcacg tattcctgta gaagttgaat
     2341 atgcttctga attccgctac cgcaatccaa tcatcaccga aaacgatgtg gtaattgcta
     2401 tcagtcaatc gggcgaaact gccgatacgc ttgctgcaat tgagttagct aaatcgaaag
     2461 gtgctaccat cttcggaatt tgtaatgttg ttggatcttc catcgcacgt gcaactcatg
     2521 ctggttcata tacacatgct ggtcctgaga ttggagttgc ttctaccaaa gctttcacag
     2581 ctcaggttac cgtgttaacg ctaatggcat tggttattgc gaataagaaa ggaacaatca
     2641 ctcaagctaa atatcacttg atgttgaata gcttggatca aatccctgct aagattgagg
     2701 aaatgttgaa actggatgat caaatccgct acatcagctc tatctataag gatgcgaata
     2761 acttcctata ccttggacgt agttacaact tcccaatcgc gctcgaaggt gcattaaaac
     2821 tgaaagaaat ctcctacatc cacgcagaag gttaccctgc tgctgagatg aaacatggtc
     2881 cgattgccct tatcgacgaa aacatgccag tggtggtaat cgctccaagt cgtggttcgt
     2941 atgagaagat tttaagcaat gtgcaggaag taaaagctcg tggtggtaaa atcattgcta
     3001 tcgttactca aggcgataaa gatgtatctg cattagctga tcatactatc gaaattccag
     3061 atgttgaaga agaattaact ccacttatga gtgtgattcc attgcaatta ttggcatacc
     3121 acattgccat tatgcgtgga tgtaacgtag accaaccaag aaatttggcc aaatcggtta
     3181 ccgtggaata acgaaatacc aaaaacaaga aagcccgaaa gattaagttc cttcgggctt
     3241 ttttatgtgt tcaaatcaga ctaaacagtc atgatttctt tctcttttgc ttcgagatgt
     3301 ttttccgttt tcgcgatata cgaatcggta agttgctgaa ttttaacttc tgcatctttc
     3361 gccacatctt cagctaaacc gtcttttact agtttacgga tagcttcatt cgcatctcgg
     3421 cgaacattac gaatactaac ctttgcgttt tcagcttcgc cttttgattg tttaaccaag
     3481 tttaaacgac gttcttccgt aacaggagga atgttcaagc gaatcataac accatcgttt
     3541 tgaggtgtta atccgatgtt agaaccgaaa atagctttct caattggacc taacatttgc
     3601 ttttcccaag gctgaacagc taaggttcgt gcatcaggag tgttgatgtt ggcaacttga
     3661 ctcaatggtg tattggaacc gtaatagtcc acataaatac catcaagcat agcaggacta
     3721 gctttaccgg cgcgaatttt agccaattct gcttctaagt gagcaattgc tttatccatc
     3781 agctgagtag ctgaatcaat ttggggctta acgagttcgt tcatgacaat tcaaaaaggt
     3841 gtgcaaagaa acaagatttc ggctgtattt tcaatacgta tcagaattca accaatgttc
     3901 caacgcgttc accatcgatt aagcgtttta ggtttccgct cgaatccata tcaaacacga
     3961 taatgggcag tttgttatcg ttacacaaag taaatgcagt catatccatt acgtttagtc
     4021 ctttttcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncta ttgataacgg
     4141 ttgcaagcat tcccatataa tcgccttgaa cacgatccat tccaccctcg gcagcttgta
     4201 ttccacggaa aatattacct ccgccaataa caacagctac ttccactcca gtagcttgga
     4261 tttcttttat ttcttgtgcg tattgcgata gacgatcgga gtcgattccg aaattcttgt
     4321 tacccatcag ggattcaccg ctaagtttca ggaggatacg tttatatttc atggttgtga
     4381 ggttcagtag ttcaaaaaaa atcccgctac gaagaacggg atgaaagagc aaaaaaaaag
     4441 agagaattac ttctctcttt tcaattcgat taattaccta aagcgtgacg tttgaaagcg
     4501 gtaacggtta ggtctttatc aattgtctga atatattggc gaacagacat tttgttatcc
     4561 ttcacaaatt cctgattaag gagtgtgttg tctttgtaaa acttattgag tttacccgca
     4621 gcgattttct caaccatttc ttctggttta ccttctgcac ggatttgctc acgagcgatg
     4681 tctaactcac gctctaaagt atgcgtatca acatcgtctt tgtcaacagc tacaggagcc
     4741 attgctgcag cttgcataga aacgtcttta gcaacagttt caattccagc aacaccagct
     4801 ttgttaaaag caacgatagt agctaggcgg ttacctgggt gattgtaagc aacaacgaaa
     4861 ggagcttcta attgagagta atgaagttcc attttctctc cgattttacc aacttgctcg
     4921 ttgatacgct cgctgatagg aaggccatca aatgaagccg ctagtaaagc gtcaacatca
     4981 gctggtttcg tgttaagggc gatatcagag attttagttg tgaaatcgat gaagtcttga
     5041 ttcttagcta cgaaatctgt ttcgcaatta agaacaacta gaacagcacg agaattatca
     5101 gcagaaacgc gagcaataac aacaccttca ttagcatcgc gatcggcacg gttaccagct
     5161 accttctgac cttttttgcg aagaaaatca actgctgctt cgaagtcacc attggcttca
     5221 gtaagcgctt ttttgcagtc catcattccc gcaccagtca tttggcggag tttatttaca
     5281 tcactagcag taattgctac acttgacatg atatgcgtgt ttcgagatta ttattagtct
     5341 tttggagttg agtcagcagc tgcagcagtt tctgctttag catgttgttc gtcatcacct
     5401 ttttctttct cttgtttgcg ttcgctcaat ccttcttgaa cagcagcaat aactacatcc
     5461 ataataagag agatagattt tgcagcatca tcatttgcag ggattggata gtcaaccaaa
     5521 ttaggatttg agttggtatc tacgatagca aatgttggga tattcaggcg acgagcttct
     5581 gctactgcga tatgctcttt agagatatct acaatgaaca aggcagctgg aaggcgactc
     5641 atatcggtaa tagaaccgaa ttgagtttcc aatttttcac gctcacgaga aatagttaat
     5701 ttctcttttt tagaaatggt ttcgtatgta ccgtcagcag ccattttatc gatggtagcc
     5761 attttcttaa ctgctttgcg gatagttgca aagttagtta gcattccacc tggccaacgt
     5821 tcagtaacat aaggcatgtt cacctttttc accgactcag ctacaatctc tttcgcttgt
     5881 ttcttagtag caacgaaaag gattttttta cccgatttag cgacttgttt caaagcagct
     5941 gcagcttctt caagcttaac aacagttttg tttaggtcaa taacgtgaat cccgttacgc
     6001 tccatgaaga tatacggagc catgtttgga ttccacttac ttttcaagtg accgaaatgt
     6061 actccggcct ctaaaagttg atcagttgat attcttgcca tggtaatagg agggtcggat
     6121 gattaacgtt tactgaactg gaagcgttta cgagctttct tctgacctgg ttttttacgc
     6181 tccaccatac gtgggtcacg tgtaacaagt ccattggtac gaagtgctgg tttgtttgca
     6241 gcatcaagtt caactagtgc ttttgcaata gcaagactca aagctccggc ttgaccagta
     6301 attccaccac cctgaatgtt agcagttaca tcgaacttgc cttcatgctc agtaactagg
     6361 aatggttgaa gaacctttcc acgaagagtt ggaacagtaa agtaatcaga gatatcgcgt
     6421 ttgttgatcg taatctttcc agtacctggt ttaagataaa cacgtgcaac agcagttttt
     6481 cttcttccgc tagtgttgat agtttccatt atcgaagatc gttaatgtta attgtttttg
     6541 gttgctgcgc tgcatgtgga tgctcgcttc cagtatacac gaataagtta ccgaataaag
     6601 cacggcctag acggcttttt ggaagcatac ctttcaccgc tttttcgatg atagcctccg
     6661 gctttttatt caacaactca cgtggagtct gaaaacgctg tccgcccgga taaccggtgt
     6721 agcgggtgta gaccttctcg tcggttttat taccggagag aacgatcttc tcagcattga
     6781 taatgattac attatcacca caatcaacgt gcggtgtata attgggcttt ttcttgccac
     6841 ggatcagcat tgcgacctgt gagctcaaac gcccaaggac catgttctcg gcgtctacca
     6901 gtacccattc cttggttact gtagccttgt tggccgaaac tgttttataa ctcagtgtat
     6961 ccacttgttt cttaggattt aagaatacga ttttccaaac gggctgcaaa gataggaata
     7021 gaatcgttat gcgcaaattt tatcaacagt aaaattcaca cggtttaatt caatacgtct
     7081 atttcctccg caaatgcctg taatgaaccg attcagcgat ttcgatttct tttattttta
     7141 ttaacactgt ggtaaatttt tagctcacca agagattaca gccgcgtaaa tcgctcgtat
     7201 caaaccgccc aattacatca aacgtcccat ctttcccctt aactcccaaa tcttgggtcg
     7261 caatgaatga acaagaatcc agattggcta aatcaatcac attaattcct ccactctttc
     7321 ctgcttctac atatgtaaat ggatcgttcg catcccgaat atgaatgtcc atccaaggcg
     7381 gacaatgaaa tcttcccgaa tcattcatcc atgcctgact gaataattcc gtcataccat
     7441 attcgctgtg caaatgtgca acgggccagg tttcgcggat agttgagtgt aattcttctc
     7501 gaatcatctc cttgcgcctt cctttcatcc ctcctgtttc tactacagta agctcgcgcc
     7561 aaacgggtgg tgcaagtaat gctaaatcga gcagggcgaa actcactccg ataagccaaa
     7621 cacgttgatt ctggcttgcc attcgtttca atacgtccaa taattcactc agattattca
     7681 aatagaatcc actttctgcc ctacccgact gacgaatcaa tccttcagtc atatatacta
     7741 aggaagaacc tgggcgttcg aggtatgctg gaagcaatcc taaaataacg gaattggatg
     7801 gtgagccata ttcacgaata aagccctctg tgaaactctc ctcatataat aaggtgtcgg
     7861 cgacgtgatg aatggaggta gtcatgccgg ttgttcccga actgctgaat tccaataccg
     7921 acgaagtttg tgagttgaat actcgttgat ccttgaaaaa ctgaatcggt aaatagggaa
     7981 tgtcgttcgg atgtgtga
//
LOCUS       DAOOQW010000099         7930 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_11281_length_7930_cov_6.170413, whole genome shotgun sequence.
ACCESSION   DAOOQW010000099 DAOOQW010000000
VERSION     DAOOQW010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 7930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7930
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11281_length_7930_cov_6.170413"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="PK281_06890"
     CDS             complement(<1..302)
                     /locus_tag="PK281_06890"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOY49109.1"
                     /translation="MGNNTDKYTFDLVVIGGGAAGFFGALQTLSRKPKARVLLIEKST
                     KLLAKVKVSGGGRCNVTNSLSDKKEFSKRYPRGEKHVFRLLHQFGQDDTVRWFEERG"
     gene            621..1493
                     /gene="sucD"
                     /locus_tag="PK281_06895"
     CDS             621..1493
                     /gene="sucD"
                     /locus_tag="PK281_06895"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009091813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HOY49110.1"
                     /translation="MSVLVNKNSKIIVQGFTGSEGTFHAGQMIDYGTLVVGGVTPGKG
                     GSSHLDRPVFNTVAEAVSKTGADVSIIFVPPAFAADAIMEAADAGIKVIICITEGIPV
                     KDMIMAKEYLNGKECRLIGPNCPGVITPEEAKVGIMPGFVFKKGKVGIVSKSGTLTYE
                     AADQVVKAGMGISTAIGIGGDPIIGTTTLEAVQLLMADPETAGIVMIGEIGGNLEADA
                     ARWIKANGTKPVVGFIAGKTAPAGRRMGHAGAIVGGADDTAEAKMRIMRECGITVVDS
                     PAVIGITMAQVLNN"
     gene            2085..3437
                     /gene="purB"
                     /locus_tag="PK281_06900"
     CDS             2085..3437
                     /gene="purB"
                     /locus_tag="PK281_06900"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HOY49111.1"
                     /translation="MTTSLEALSPIDGRYLRYTASLRPYFSELALFKYRVQVEIEYLI
                     ALSEIGLKGFEPFTDSEKAQLRLIYQEFGKDHGAAIKEIEKRTNHDIKAVEYFIKEQL
                     EQLGLGAKMEWVHFGLTSQDINNTAIPLSMKEAAEQVIFPALEELCAELKKLANTWKD
                     LPMLARTHGQAASPTTLGKEIYVFAERIEIQIQQLKQTVWAAKFGGATGNFNAHHVAF
                     PSTDWLNFAHGFVSSLGLSRSRTTTQIEHYDHLAAYCQNMMRVHTILIDFCRDIWMYI
                     SMEYFRQRVNPGETGSSAMPHKVNPIDFENAEGNLGIANALLDHFAQKLPISRLQRDL
                     TDSTVLRNIGVPFAHSLISIHSIQRGLGKLLLNESAIAADLNHHTAVIAEAIQTILRR
                     EGYPQPYEALKGLTRGNNTIDLPLIHEFIRNLNVSDAVKAELLQITPANYTGIIRFEA
                     "
     gene            3434..4306
                     /locus_tag="PK281_06905"
     CDS             3434..4306
                     /locus_tag="PK281_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOY49112.1"
                     /translation="MKVSGFTFIRNAVKFDYPVVEAITSVLPMVDEFIVMAGNSEDST
                     NELIASIPSDKIKIFHSVWDDSLRSGGRVLALETDKAMEHISADSDWAFYIQADEVVH
                     ETFHAQALESMRRWKDDKRVEGLLFNYTHFYGSYDFIGDSRRWYRKEVRIIRPHIGVQ
                     SWKDAQGFRIDGKPLKVKESGASIYHYGWVKPPEQQQAKQEYFHKLWHDDQWMKENIP
                     EVAEFDYSSIDSVARFEGTHPSVMQARVDRQNWKVNIDPAKKKLSVRYKFLLFIERLT
                     GWRAGEYKNYRILK"
     gene            4418..6667
                     /gene="purL"
                     /locus_tag="PK281_06910"
     CDS             4418..6667
                     /gene="purL"
                     /locus_tag="PK281_06910"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HOY49113.1"
                     /translation="MQTTNANTALTTVDTAKKLGLLPEEFERICTILGRTPNFTELSI
                     FSVMWSEHCSYKNSITWLKTLPRDGEALLAKAGEENAGLIDIGDGWACCFKIESHNHP
                     SALEPYQGAATGVGGINRDIFTMGARPVAQLNSLRFGDPALAKTQWLVRGVVKGIGNY
                     GNAFGIPVVGGEVFFDECYNVNPLVNAMSVGLVRHGETASATSEGVGNPVYIVGSSTG
                     KDGIHGATFASGDLTEESAKDLPAVQVGDPFQEKLLLEATLEVIKTGAIIGMQDMGAA
                     GITCSTSEMSAKGEHGMDIWLDKVPTRQDGMHGWEILLSESQERMLMVVKKGREEEVE
                     AVFAKWDLHYAVIGIVTEGNRLRYYMKGELEADVPAFDLVLGGGAPVYKREYTEPAYF
                     AEAQKFSIDQITEPSDLNQVAKHLLANPNIASKRWVYEQYDSMVGTANMSTNRPSDAA
                     LVNVKGTNRALALTVDCNSRYVNADPEKGASIAVAEAARNIVCSGGKPMAITNCLNFG
                     NPYVPEVYWQFVKAIQGMGAACKAFGTPVTGGNVSFYNQTQVGKETIPVFPTPTIGML
                     GLLEDKTQGMGLAFQNEGDLIYLIGASRNDINASEYLASWHNVRQSPVPYFHMEEELD
                     MQQTVSQLIGAELVNSAHDVSDGGLFTTLAESGFVNNLGFTISTDEDFRKDAFLFGEA
                     QGRVVVSIAPGKQEEFVEFMAMGEVPFSLLGTVTTGEVWVDDEFWGSLSEFAEGYDSA
                     LGKIMDQHV"
     gene            6660..7358
                     /gene="radC"
                     /locus_tag="PK281_06915"
     CDS             6660..7358
                     /gene="radC"
                     /locus_tag="PK281_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014782620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HOY49114.1"
                     /translation="MSENDNYRPIREWNEDDRPREKLQLKGKSNLSDAELVAILLGSG
                     TRRESAVELARRVLHLADNNLLELGKLSLTQLQKLPGIGPVKAITLTAAFELGRRRQE
                     SEPVRRGQVLNSRDIYNIFGPRLGDLRHEEFWIMCLNRNNRILGTSRIGAGGISATVA
                     DVRLILHYAIERHASCLILCHNHPSGNTTPSDADIKLTHSIQQAAKYMDIVLLDHLIV
                     TDSSYFSFADESKL"
     gene            7360..>7930
                     /locus_tag="PK281_06920"
     CDS             7360..>7930
                     /locus_tag="PK281_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011708438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HOY49115.1"
                     /translation="MAKAPQNIHSKEVIEKIKASRAVRETVDQLGKSVIQGDTNALAR
                     AITLIESSHEGDSKQAEELLRMVLPFSGKSVRVGISGVPGAGKSTFIEAFGMHLIRQG
                     KKVAVLAVDPSSEAGKGSILGDKTRMNSLASSELAFIRPSPSSGALGGVARKTREAIF
                     LCEAAGFEYILVETVGVGQSETAVHSMVDF"
BASE COUNT         2248 a         1500 c         1909 g         2273 t
ORIGIN      
        1 ccgcgttctt cgaaccaacg aacggtgtcg tcttgtccaa attgatgtaa caaacgaaac
       61 acatgttttt cgcctcgcgg atagcgtttg gaaaactctt ttttgtcgga caaactattg
      121 gttacattgc atcttccgcc acccgaaacc ttgactttcg ccagtagttt cgtcgatttc
      181 tcaattaaca atacacgtgc tttgggtttt cggctcaagg tttgaagtgc cccgaaaaat
      241 ccagcggcac ctccaccaat cacgaccagg tcgaaagtgt atttgtctgt attattgccc
      301 attaggtttt tattaacatc aattaacatt tcaatcgcta atcgcacggc gaagttctgt
      361 atttttaaat gttttcaatt gtttttcatt tttgtttcta tttgattcca gttcaaactc
      421 tcggtcttcc caccgggagt ttttttgttt tgggcggttt gaatccagat aggttattga
      481 aatttctcta gaaaaagatt gcagattcac gaattgtgcg caatagcaag cggttgcaga
      541 aaaccagaaa tcaatcttga ttcatcatcc gaacaagtct ttttttccgt acgtttgccc
      601 ccgaaatttc gaacatactc atgagcgttt tagtcaacaa aaacagtaaa atcatcgtac
      661 aaggatttac cggtagcgaa ggaacatttc acgccggaca aatgatcgat tacggtacac
      721 tggtagtcgg cggagttact ccaggaaaag gtggaagttc tcatctcgat cgcccagttt
      781 ttaatactgt agccgaagcc gtttctaaaa caggcgctga tgtgtctatc attttcgttc
      841 cgccagcatt tgctgccgat gcgattatgg aagctgccga tgctggaatt aaagtgatta
      901 tctgcatcac cgaaggaatt ccggtgaaag atatgatcat ggcgaaggaa tatttgaatg
      961 ggaaagagtg tcgcttgatt ggaccaaatt gtccgggagt gattactcca gaagaagcga
     1021 aagtgggcat catgccagga tttgtgttta agaaagggaa agtgggaatt gtttctaaat
     1081 cgggaacatt gacttacgaa gctgccgatc aagtggtaaa agccggaatg ggaatttcta
     1141 ctgcgattgg aattggtgga gatccgatta ttggaactac cactttagaa gctgttcaac
     1201 tattgatggc tgatccagaa actgccggta ttgtgatgat tggtgagatt ggtggaaatt
     1261 tagaagccga tgctgctcgt tggattaaag caaacggaac taaacctgtt gtcggattta
     1321 ttgctggtaa aacagctcca gcaggtcgtc gtatgggtca cgccggtgct attgttggcg
     1381 gtgcagatga tacagctgaa gctaaaatgc gcatcatgcg tgaatgtgga attacggtag
     1441 ttgattcgcc tgcagtcatt ggtattacga tggctcaagt gcttaacaac taattgaatt
     1501 taactaacct tctttacggt tgaattaaca gtttagtatt tcatatcaat ccttgaaata
     1561 aggctgttaa agcatcttgt cattcaatca tacatgattg tctacgacgt gttaattctt
     1621 gtcttgacac acacttcgac aagctcagtg tgcgagctaa gaaggtcaag cttggacgat
     1681 gctgcttacg cgcaggtgta ttcactaaac gacctcaccc ccaacccctc tcctcgagga
     1741 gaggggagct tgattgtctt aaatgaaaag tccctctacc gtggagaggg atttagggag
     1801 aggtcaacgc tgggaaacac accctcgccg cccagacata gccttcgcac tgatccgttt
     1861 ctaaacctaa aataggttaa tttaatttta tataatagtc atctacttac cttgagtatt
     1921 ggacttgcta ttagaatcaa tgaagaataa tattaaattt taaactgctc aacaggttct
     1981 aacttatata attaatgcct gttcttatcc atcgtgtttt tctatttcta taagtcacca
     2041 agtaaacgtt ttttttgacg ttactttgca ccttacatac taccatgaca acatctttgg
     2101 aagcgctttc acccatcgac ggtcgatatt tacgctatac cgcttcatta cgcccttatt
     2161 tttctgagct cgccttattt aaataccgcg ttcaggttga aatagaatac ctcattgctt
     2221 tatcggaaat aggattgaag ggatttgaac cgtttacgga ttcggagaaa gcgcagcttc
     2281 ggttgattta tcaagaattt ggaaaggatc acggtgctgc gattaaggaa attgagaagc
     2341 gtaccaatca cgatattaaa gcggttgaat atttcatcaa ggaacaactg gagcagcttg
     2401 gtttaggggc gaaaatggaa tgggtgcatt tcggcttaac ttctcaggat atcaataata
     2461 ccgcgattcc actttcgatg aaagaagcgg ctgagcaagt tattttcccc gctttagaag
     2521 aattatgtgc tgagttgaaa aagcttgcaa acacttggaa agacttaccc atgctcgctc
     2581 gtactcacgg acaggcggct tcacctacta cattaggaaa agagatttac gtttttgcgg
     2641 agcgaattga gattcaaatt cagcaactca agcaaactgt ttgggcagct aaatttggcg
     2701 gtgcaacggg gaatttcaat gcgcatcatg tagcatttcc ttcgaccgat tggttgaatt
     2761 ttgcccatgg atttgtgagt tcactaggat tgtctcgaag ccgcaccacg actcagattg
     2821 agcattatga tcatctggct gcgtattgtc agaatatgat gcgtgtgcat accatactca
     2881 tcgatttttg tcgggatatt tggatgtata tctcgatgga atattttcgt cagcgagtaa
     2941 atccgggcga gacaggttct tcggctatgc cacataaagt gaatccgatt gatttcgaaa
     3001 atgccgaagg aaatttggga attgcaaatg ccttgttaga tcattttgct caaaagcttc
     3061 ctatttcaag acttcagcgc gatttaaccg attcaaccgt attgcgaaat atcggagttc
     3121 catttgcgca tagtttaatt tcgattcatt caattcagcg tggattaggt aagcttttat
     3181 tgaatgaatc agccattgca gccgatttga atcatcatac agccgtgatt gccgaagcca
     3241 tccaaaccat tttgcgtcgc gaaggatatc ctcaacctta cgaagcgcta aaaggcttga
     3301 cacgtgggaa taacacgatt gatttgccac tcattcacga attcatccga aacttaaatg
     3361 taagtgatgc agtgaaagcg gaattgcttc aaattacacc tgccaattac accggaatta
     3421 ttcgtttcga ggcatgaaag taagcggttt cacctttatc cggaatgctg taaaattcga
     3481 ttatccggta gttgaagcta ttacctctgt cttgccgatg gtggatgaat ttatcgtcat
     3541 ggcgggcaat tcggaagatt ccaccaatga attaattgcc tccattcctt ccgataagat
     3601 taagattttc cactcggttt gggatgatag tttacgcagc ggaggacgag tgttggcgtt
     3661 agaaaccgat aaagccatgg agcatatttc ggccgacagt gattgggcgt tttacattca
     3721 ggccgatgaa gtggtgcatg aaaccttcca tgctcaagcc cttgaaagta tgagacgttg
     3781 gaaggatgat aaacgagtag aaggcttgct ttttaactac acccattttt atggatcgta
     3841 cgatttcatt ggcgattcga gacgttggta ccggaaagaa gtgcgcatta ttcgtccgca
     3901 tattggtgtt caatcttgga aagatgctca aggctttcgg attgatggca agccattgaa
     3961 agtaaaagaa agcggagcta gtatttatca ctacggttgg gtgaaaccac ccgaacaaca
     4021 acaagctaag caagaatatt ttcacaaact ctggcacgat gaccagtgga tgaaagagaa
     4081 cattcccgaa gtagctgaat ttgattattc aagtatcgat tcagtagctc gttttgaagg
     4141 tactcatcca tcggtgatgc aagcccgagt tgaccgtcag aactggaaag taaacatcga
     4201 tccagctaaa aagaaattgt ctgttcgcta taaatttctg ctgtttatcg agcgactaac
     4261 aggttggcgc gcgggtgaat acaagaacta ccgaattctg aaatagcgat ttttctcagt
     4321 tatcgactaa gtttctaaca gcactgttca tattcctttg ttgtttcgac tatgttccgc
     4381 ttggtgcttt tcgtaggttt gcaaaccgat cttcgtcatg caaacaacca acgccaatac
     4441 cgcccttaca actgttgata ccgctaaaaa actcggatta cttccggagg agtttgagcg
     4501 catctgtacc attctcggac gaacacccaa ttttacggag ctgagtattt tctcggtaat
     4561 gtggagtgag cactgttctt acaaaaattc gattacctgg ttaaagactt taccccgcga
     4621 tggggaagcg ttattagcta aggcaggaga agagaatgcc ggattgattg atattggtga
     4681 tggctgggcg tgttgtttca agatagaatc gcacaatcac ccttcggcat tggaacctta
     4741 tcaaggagca gctaccggag ttggtggaat taaccgcgat atttttacca tgggcgctcg
     4801 tccagtggcg caattaaact cgctacgttt tggtgatcca gctttagcta aaactcagtg
     4861 gctcgttcgt ggtgtggtga aaggaattgg aaattatggg aatgcctttg ggattccagt
     4921 tgtgggtgga gaagtattct ttgacgaatg ctacaatgtg aatccattgg tgaatgcgat
     4981 gtcggttgga ttggttcgtc atggcgaaac agcatctgcc acatccgaag gtgttggaaa
     5041 tccagtttac attgttggtt catccacagg aaaagacggg attcatggag caacattcgc
     5101 ttcaggtgat ttaacagaag aatcagctaa agatttacca gccgttcagg taggtgatcc
     5161 attccaagag aaactattgt tggaagctac cttagaggta attaagacag gagctattat
     5221 cggaatgcaa gacatgggag cagcaggtat tacttgttca acttcggaaa tgtcggctaa
     5281 aggtgagcat ggaatggata tttggttgga taaagttcca actcgtcagg atggaatgca
     5341 tggttgggag attttacttt ccgaatctca ggaacgcatg ctgatggtgg taaagaaggg
     5401 tagagaagag gaagtagaag ccgtatttgc taaatgggat ttgcattatg cagtaatagg
     5461 aattgtgacg gaaggcaatc gtttgcgtta ttatatgaaa ggtgaattgg aggctgatgt
     5521 tccagcattt gatttagtat taggtggcgg tgcaccggtt tacaaacgtg agtatacaga
     5581 accagcctat ttcgcagaag cacagaagtt ttcgattgat caaattacag agccaagcga
     5641 tttgaatcag gtagccaagc atttgttagc taatccaaac attgccagca agcgttgggt
     5701 gtatgagcaa tacgattcga tggtaggaac tgcgaatatg agcaccaatc gaccatctga
     5761 tgcagccttg gtaaatgtga aaggaactaa cagagcattg gctttaacgg tagattgtaa
     5821 ttcacgttat gtgaatgcag atccagaaaa aggagcttct attgctgttg cagaagccgc
     5881 acgaaacatt gtctgctcag gcggaaagcc aatggccata accaattgct tgaattttgg
     5941 gaatccgtat gtgcccgaag tttactggca gtttgtgaaa gcaattcaag gtatgggagc
     6001 tgcttgtaaa gcatttggaa cacccgttac tggtggaaat gtgagtttct acaatcagac
     6061 tcaggtgggt aaagaaacaa tccctgtttt cccaactcct actattggaa tgttaggttt
     6121 attggaagat aaaacacaag gaatggggct agcatttcaa aatgaaggtg atttgattta
     6181 cctcattggt gcgagtcgta acgatatcaa tgcttcggag tatttagcat cctggcataa
     6241 tgtgcgtcaa agtccggttc catatttcca catggaggaa gagctcgaca tgcagcaaac
     6301 cgtttctcaa ttgattggag ctgaattggt gaattcggcg cacgatgttt ctgatggagg
     6361 tttgtttacc accttggctg aatcgggctt tgtgaataat ctaggattta ccattagtac
     6421 agatgaggat ttccgtaagg atgcattctt atttggtgaa gcgcaagggc gagttgtagt
     6481 aagcattgca ccaggtaaac aagaagaatt tgttgagttt atggcaatgg gtgaagtacc
     6541 atttagcctt ttaggaacgg taacgactgg tgaagtttgg gtagatgatg aattctgggg
     6601 ctcattaagt gaatttgccg aaggatatga ttctgcttta gggaagatca tggatcagca
     6661 tgtctgagaa cgacaattac cggccaattc gtgagtggaa cgaggatgat cgtccgcggg
     6721 aaaaactcca attgaaaggt aaaagtaatc tcagtgatgc cgaactagtt gcaattttat
     6781 tgggaagcgg aacacgtcgg gaaagtgcgg tggagttggc aagacgggtt ttgcatttgg
     6841 ctgacaataa tttgttggag ctagggaaat taagtttgac tcagcttcag aagttacccg
     6901 gaattggtcc tgttaaagca ataaccttaa cagcagcttt tgaactgggg cgcagaagac
     6961 aagaatctga gccagtaaga cgcggacagg ttttgaatag tcgtgatatc tacaacattt
     7021 tcggtccacg attaggagat ttaaggcacg aagagttttg gattatgtgc ttaaatcgga
     7081 ataaccgaat tctaggaacc agccgaattg gagccggagg aatttcggcc actgtagcag
     7141 atgtgcgatt aatacttcat tatgctattg aacgccacgc gagttgtttg attctatgtc
     7201 ataatcatcc ttcgggaaac accacaccga gtgatgcaga tatcaaacta actcactcca
     7261 ttcaacaggc tgcgaagtat atggacatcg tacttttgga ccacttgatt gttaccgatt
     7321 cctcgtattt tagtttcgct gacgaaagca agctctaaca tggccaaagc accgcaaaat
     7381 attcatagca aggaggttat tgaaaaaatt aaagcgagtc gtgccgttcg ggaaactgtt
     7441 gaccagcttg gaaagagcgt aattcaaggt gatacaaacg cattagctag agcgattaca
     7501 cttatagaaa gttcgcacga aggagatagc aaacaagcgg aggaattgct tcgaatggta
     7561 ttaccatttt cggggaagtc tgtacgggtt ggaattagtg gagtaccagg agctggaaaa
     7621 agtacattca ttgaagcatt tggaatgcat ctgattagac aagggaagaa ggttgcagtt
     7681 ttggctgttg atcccagttc agaagctgga aaaggaagca ttttgggaga taaaactcgt
     7741 atgaatagtt tggcttccag tgagttggca tttattcgtc cgagtccgag ttcaggagcg
     7801 ttgggaggag ttgcccgcaa aacacgtgaa gcaattttct tatgtgaagc tgcaggtttc
     7861 gaatatattc tggtcgaaac tgttggcgtt ggacaaagtg aaacagcagt gcattcgatg
     7921 gtcgatttct
//
LOCUS       DAOOQW010000100         7882 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_11370_length_7882_cov_4.912738, whole genome shotgun sequence.
ACCESSION   DAOOQW010000100 DAOOQW010000000
VERSION     DAOOQW010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 7882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7882
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11370_length_7882_cov_4.912738"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="PK281_06925"
     CDS             complement(<1..470)
                     /locus_tag="PK281_06925"
                     /inference="COORDINATES: protein motif:HMM:NF012702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HOY49116.1"
                     /translation="MQLNTRRPRRNRRSEAVRSMIQETRLHPSQLILPFFLIEGENRK
                     VEIASMPGVFRWTIDLAVKEAAECMTLGIRNFILFPAIDESKKDKYATWSYHPDCFYL
                     KAIQAFKTALPDCSVTTDVAMDPYSSDGHDGIYENGIILNDQTLEVLGKMSVAQA"
     gene            703..>1382
                     /locus_tag="PK281_06930"
     CDS             703..>1382
                     /locus_tag="PK281_06930"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOY49117.1"
                     /translation="MNIHSHSGFKKVEDSSIYENWVERVEKQNGIRYERLSLTTTLGN
                     TTVYVRPSDAQESLVIFPGFRTSSLFWDLDNGLVELAKHFRIYLVETNGQPNTSEGNT
                     PKIKTLEYGDWALEVLKGLGLTKTHIAGASFGGTVALKLASVAPEFIDKVILLNPGCL
                     QNFSLKWKNLWLNMLPILSPKRANIVRFLNEIVLCPPTQTLSNEAFELLVEYQEMALK
                     LWSDKAQKP"
     assembly_gap    1383..1482
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1483..2893
                     /locus_tag="PK281_06935"
     CDS             <1483..2893
                     /locus_tag="PK281_06935"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOY49118.1"
                     /translation="FFLSMKRKLLSRPFLITYAVVLVLIAWFVLSGEAAGEKQPIFAD
                     VQSGDFEISVTTTGEIRTKTQTDIDAPGPEMQEAGLWNGTKIEDLVPEGTVVKAGEFV
                     ASLDKSALMTKLQEISLEFDKKNSELKQARLDTSITLRDARDELLNLKSAKEEARLEL
                     EQSAYEAPAIQRQKQLALEKADRNYTQKVDNYKTKVEQSATKVAIIHADMMKAQNSLN
                     RVNSLLNKMVITAPKDGMVIYVKDWNGKKRGVGSTVNPWESSVATLPDLREMQVITYV
                     NEVDIRKIKAGQAVSIKLDSDPSKNLKGVVVQVANIGEQRPNQDSKVFEVLIDVLTKD
                     PSLRPSMTTSNIIHIDAFKNVLSVPLEAITTEKKHSYVWVKDGASYRKQEVRVGAVND
                     MAAMVYSGLKSEETVFLTVPSDTSGSELIRTDTTIKPPAPFIDKKEQELLQNHLKTSV
                     TATKRGAGEISGGMTIVIE"
     gene            2899..4251
                     /locus_tag="PK281_06940"
     CDS             2899..4251
                     /locus_tag="PK281_06940"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOY49119.1"
                     /translation="MEFERFIFNFRIGAEALITNRFRAILTSLGIIFGVAAVIAMLAI
                     GAGTEKEIIEQIKQIGSNNIVIKSRVEKKKNLEEDPTVQAKQENGKYSPGLTLADAEN
                     ILQTIPNVNFVSPEIESEMQFYSEGLITSGNLIGITNHYFETNNLLVNEGLMFSDDQL
                     ASSAPVCIIGRGVKAKLFSKTEPIGQRLKCGGTWLTVVGVLENRDVSQSASQKLSIRN
                     VNTEVYIPVRTMLMRFINRSNVTVTDIKRANQQSMDDDGSQQKQVVDENYHQIDRMIV
                     NVKDSKHISATVELLEKMLSRRHNEVDDYEIQVPELLLKQEQRTRKLFNIVLAVIAGI
                     SLLVGGIGIMNIMLASVLERTKEIGLRLSIGARRRDIVFQFIAEAVMISLSGGLLGIV
                     MGVVISILIEKVAGIQTIISGASVMLSFVVSVSVGLLFGIFPAKRASEQSPMESLRYE
                     "
     gene            4244..5698
                     /locus_tag="PK281_06945"
     CDS             4244..5698
                     /locus_tag="PK281_06945"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOY49120.1"
                     /translation="MNKKLLFFFLVLLSSGLSAQSSLSLQEAIRLGMESSPIGKQIRA
                     SYESNRWQYRAARASRLPQLSLSGSAPGYTRRINQVFQPDGSFVFAPIQQAFSSGNLS
                     LSQNILATGGTLSVGSGVSRGDIFSGVNSTFYQSNPLAINLSQPLVRFNSMKWQWKQN
                     QLQMNMATRQQIENLEDLSITITQRYFDLYTANLQLKNAELNQAINDTIYKIAKGRYG
                     VGKIAENELLQVELSYTNARNEVEKQKLNIVLAERQLELLIGRPIKSSDVSPPIEIPK
                     IDPEPQLAVSEAKNNRSDLIGWQMNENQAQRAFREAQLSRRLSADMNISYGLNQTGNT
                     FDAATKNPLESQFAGINFTIPIASMGANRALQKSAQYNLEAQREQNQNNINNLEQEVY
                     ASTLQFLQLKSSLKISSKADTIAQKRYEVARNRYRIGTVDITNLLIAQNEKDQALITY
                     VSTLRDFWVAYFRLRRLTLYDFENKVRLVTESSY"
     gene            6019..6579
                     /locus_tag="PK281_06950"
     CDS             6019..6579
                     /locus_tag="PK281_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HOY49121.1"
                     /translation="MEENQELVNRIFLIRNQRVMLDSDLAEIYGVETRRLNEQFKRNE
                     IRFPKDFAFRLTAAEWTNLKSQMLVNSTESIDSIIEADDNSSQDWGGRRKMPLVFTEH
                     GAVMLASILSSEKAVEASIFVVRTFIRFRHMISTQDHLATKILQLEEKFDGQFNIVFD
                     ALRDLLEHPPLPKIRQRIGFKKDEVG"
     gene            6708..>7882
                     /gene="carB"
                     /locus_tag="PK281_06955"
     CDS             6708..>7882
                     /gene="carB"
                     /locus_tag="PK281_06955"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HOY49122.1"
                     /translation="MPKKSEIRSVLIIGSGPIIIGQACEFDYSGSQAARSLREEGIEV
                     SLINSNPATIMTDKVSADHVYLLPLTVASIVKILEERKIDAVLPTMGGQTALNLAKKA
                     DEMGIWAKYGVKIIGVEIDAIETTENREAFRQLCVDLNVGVPPSQIANSFLEGKEIAQ
                     RIGYPLVIRPSYTLGGTGGAFVHRKEDFDELLNRGLHASPTHEVLIEKALFGWKEFEL
                     ELLRDNNQNIIIICSIENFDPMGIHTGDSITVAPAMTLSDTAYQHMRDMAIKLMRHIG
                     TFAGGCNVQFAMNPENEDIIAIEINPRVSRSSALASKATGYPIAKIAAKLAIGYNLDE
                     LSNQITGTTSAYFEPTLDYVIVKIPRWNFDKFKGADKSLGLQMKSVGEVMGIGRNFQ"
BASE COUNT         2529 a         1289 c         1634 g         2330 t
ORIGIN      
        1 gcttgagcaa ccgacatttt ccctaaaact tcgagcgttt gatcgttgag gatgatacca
       61 ttttcgtaga tgccatcgtg tccatcgcta ctgtatggat ccattgccac atcagtcgtt
      121 acggagcaat ctggaagtgc tgttttaaaa gcttgaatgg cctttagata gaagcaatcg
      181 ggatgataac tccaagtagc gtatttatct tttttcgatt cgtcgatagc tgggaatagg
      241 atgaaatttc ggattccaag tgtcatacat tcggctgctt ctttcacggc taagtcgatg
      301 gtccagcgga aaacaccggg catagatgcg atttcgactt tacgattttc tccttcgatg
      361 aggaagaaag gcaagatgag ttgagatgga tgaagacgag tttcctgaat catgctgcga
      421 acagcttcgg aacgacggtt tcggcgaggg cgacgagtat taagttgcat ggtgcaaaga
      481 tgagaaaggg aagcggcctt cgacaagctc aggcaacaaa aaatgtttta gtacaaagta
      541 tagagtacaa agtataaagt acaaagtatg aagtacaaag tagaaaggga aaagtacaaa
      601 gtagtccatg cataaaatag gttgactcaa tacttaatac acaatactaa atacttcata
      661 ctcatttatg cagtattttt cgcggcccaa ttcaagacaa ttatgaatat acatagccat
      721 tccggattta agaaagttga agatagttcg atttatgaaa attgggttga gcgggttgaa
      781 aagcagaatg gtattcggta tgaacgattg agtttaacta caacattagg taatacgacc
      841 gtttatgttc gaccttcaga tgctcaagaa tcgctggtga tttttccggg atttcggact
      901 tcgtctctat tttgggattt agataatgga ttagttgaat tggctaagca ttttcgaatt
      961 tatctggtcg aaacaaatgg ccaacccaat actagtgaag gcaatactcc caagataaaa
     1021 acacttgaat atggagattg ggcattggaa gtattaaaag gattgggttt gactaaaacg
     1081 catatagcag gagcttcatt tggtggaacg gttgcattga aattagcgtc tgtagcaccc
     1141 gaatttatag ataaagtcat tttattgaat ccgggatgtt tgcaaaattt ctctctaaaa
     1201 tggaagaatt tgtggttaaa catgctaccg atactttctc caaaacgtgc taacattgtg
     1261 cgatttctaa atgaaatagt attatgtccg ccaactcaaa cactttcaaa cgaagctttt
     1321 gaattacttg ttgaatacca agaaatggcc ttaaaacttt ggtctgataa agcacaaaag
     1381 ccnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnattttttt tatctatgaa
     1501 gcgaaaacta ctcagtcgcc cctttctaat tacctatgct gttgttttag ttttgatagc
     1561 ttggtttgtc ctgagtggtg aagcagcagg agagaagcag ccaatttttg ctgatgttca
     1621 atcgggtgat tttgagatat ctgttaccac aactggggag attcgaacga aaacacagac
     1681 tgatattgac gcacctggac cagaaatgca agaagctggt ttatggaatg ggacaaagat
     1741 tgaggactta gtacccgagg gaacggtagt taaagcggga gaatttgtag cttccttaga
     1801 taaatcggcc ttgatgacca agcttcaaga aattagtttg gagttcgaca agaagaattc
     1861 agaattaaag caggcaagat tagatacatc cattacttta cgagatgcgc gagatgagtt
     1921 attgaattta aaatcggcta aagaggaagc tcgcttggaa ttggaacaaa gtgcctatga
     1981 agcacctgcc attcaacgtc aaaagcaatt ggcattagaa aaagcagatc gtaattacac
     2041 gcaaaaagta gataattaca aaactaaagt ggagcaatcg gctactaaag ttgccattat
     2101 tcatgcggat atgatgaagg ctcaaaatag tttgaatcgc gtgaattcgc ttctaaataa
     2161 aatggttatt actgctccca aagatggaat ggtgatttat gtaaaagatt ggaatgggaa
     2221 gaaaagaggc gttggaagta cggtaaatcc ttgggaatcg tcagttgcta ctttacctga
     2281 tttacgtgag atgcaagtga ttacctatgt aaatgaggtt gatattagaa agataaaagc
     2341 aggacaagca gtaagtataa agttggattc cgatccatcg aagaatttaa aaggtgttgt
     2401 tgttcaagta gctaacatag gagaacagcg accaaatcaa gattctaaag ttttcgaggt
     2461 actcattgat gttttaacta aagatcctag tttaagacca tccatgacaa caagcaacat
     2521 catccatatt gatgcattca aaaatgtgtt gtcggttcca ctagaagcta ttacgacaga
     2581 aaagaagcat tcgtatgtgt gggttaaaga tggagcaagc tatcgaaaac aagaggttag
     2641 agtaggagcg gtgaatgaca tggcagcaat ggtatactcg ggattgaaat ccgaagagac
     2701 tgtgttttta actgttccat ccgacacatc tggttcagaa ttaattcgaa ccgacactac
     2761 catcaaacct cctgcgccat tcatcgataa aaaggaacaa gagcttttac aaaatcactt
     2821 gaaaactagt gtcacagcta ctaagcgtgg agcaggtgaa attagtggtg gaatgactat
     2881 cgtaattgaa taaggcgcat ggaattcgag cgttttatat tcaattttcg aattggtgct
     2941 gaagcattaa taaccaatcg atttagagca atcctaactt ctctgggaat tattttcgga
     3001 gttgctgccg taattgccat gttggctatt ggagcaggaa cagagaagga aataattgag
     3061 cagattaagc agattggttc taataatatt gttattaaat cgcgggttga aaagaagaaa
     3121 aatttagagg aagatccaac ggttcaggct aagcaagaaa atgggaagta tagtcctggc
     3181 ttaacattgg ccgatgctga aaatatactt caaaccattc ctaacgtcaa ttttgtaagt
     3241 cccgaaatag aatcagaaat gcagttctat tcggaaggat tgattaccag tgggaattta
     3301 attggcatta cgaaccacta ttttgaaacg aataatttat tggtcaatga aggtttgatg
     3361 ttttcagatg atcaattagc tagttctgct ccagtatgca ttattggcag aggtgttaaa
     3421 gccaaacttt tttctaaaac ggaacctatt ggacaacgat tgaagtgtgg tggaacctgg
     3481 ttaacagttg ttggagttct tgaaaataga gatgttagtc aatctgcttc gcaaaaatta
     3541 tccatccgaa atgtaaatac cgaagtttac attcctgtga gaacgatgct catgcgattt
     3601 atcaatcggt caaatgtcac ggtgaccgat atcaaacgtg ccaatcaaca aagtatggat
     3661 gatgatggaa gtcaacaaaa acaagttgtt gatgagaatt atcatcaaat agataggatg
     3721 attgtgaatg tgaaagacag caagcatatt tcagccactg tcgaattatt agaaaaaatg
     3781 ctgagtcgta gacataatga agttgatgac tacgaaattc aagttcctga gcttttgctc
     3841 aagcaagaac agcgtactcg gaaattattc aatattgtat tggctgtaat tgcaggaatt
     3901 tctctgctcg taggtggaat tggaattatg aatattatgc tagcctctgt tctagaacga
     3961 actaaagaaa ttggtctacg attatcgata ggtgctcgaa gaagagatat tgtatttcaa
     4021 tttatagctg aagctgtaat gataagtttg agtggaggat tattaggaat tgtgatggga
     4081 gttgtcatta gtatattaat tgaaaaagtt gctggtattc aaacaattat ctcaggagca
     4141 tctgtgatgt tatcgtttgt ggtatccgtt tcggtgggtt tattatttgg gattttccct
     4201 gctaaacgtg cttctgaaca aagtccaatg gaatcgttac gatatgaata aaaagcttct
     4261 atttttcttc ctagtgctgc tttcaagtgg tttgtctgcc cagtcttcgc ttagtttgca
     4321 agaagcaatc cgactgggta tggagagcag tccaattggt aaacaaatac gtgcttcata
     4381 cgaaagtaat cgttggcaat atagagctgc tcgagcgagt cgtttaccac aattgagttt
     4441 atcgggttct gcacctggtt atactagacg aattaatcaa gtatttcaac ccgatggatc
     4501 atttgtattt gcgccaattc aacaggcttt ttctagtgga aacttgtcgt taagccaaaa
     4561 tattttagcc acaggaggta ctttgagtgt tggaagtggt gttagccggg gagatatttt
     4621 tagtggtgtt aattctacct tttaccaatc taacccattg gcgattaatt tatctcagcc
     4681 attagtgcga tttaatagta tgaaatggca atggaagcaa aaccagcttc aaatgaatat
     4741 ggctactcga caacagattg agaatttaga ggatttatca attaccatta ctcaacgata
     4801 tttcgactta tatacagcta atttgcaatt gaaaaatgca gagctcaatc aagctattaa
     4861 tgatacaatt tacaaaatag ctaaaggtcg atatggtgtt ggcaaaattg ctgaaaatga
     4921 gcttttgcag gtagaactaa gttatactaa tgctagaaat gaagttgaaa aacagaaact
     4981 aaatatcgta ttagcagaaa ggcagcttga attattgatt ggtcgcccga ttaaaagttc
     5041 cgatgttagt ccacccattg aaattcctaa aatcgatcct gagccacaac ttgcagtttc
     5101 agaagcaaag aataatcgga gtgatttaat tggttggcag atgaatgaga atcaagctca
     5161 acgagcgttt cgagaagcac agttaagtcg tcgattatca gccgatatga atataagtta
     5221 cgggctaaat caaacaggaa atacatttga tgcagctact aagaatccac tagaatctca
     5281 gtttgcagga attaatttta ccattccaat tgcatcgatg ggagctaatc gagcattgca
     5341 aaaatctgct caatataact tagaagctca acgtgaacag aatcaaaata atatcaataa
     5401 tttggaacaa gaagtctatg cttctacctt acagtttctt caattaaaat caagtttaaa
     5461 gatttcttca aaagccgaca caatcgcaca aaaacgatac gaagtagctc gtaatcgtta
     5521 cagaatagga actgtcgaca taaccaattt actcattgca cagaacgaga aagatcaggc
     5581 attgataact tacgtaagca ctttacgtga tttttgggta gcctatttcc gattgagaag
     5641 gctaactctg tatgatttcg aaaataaagt tcggctggtt actgaatctt cgtattaatt
     5701 catttgttca aatttaggtg cttttttcac cacctaaaat acgctacctt gaaatcgcat
     5761 tttgcgacat caagttctgg aatcattgat attattgagt tgtaagagtt tttgttaatc
     5821 tattataaac atagaagatc aaattcggta gttattgata ctggctattc tgatttttat
     5881 atttttcatt gaacttacat tttaagggat tgattttctg gttgatactt attttaaacg
     5941 tttataaacg tatataaatg tttcattata agcatattaa tttcagattt ttcaaattgc
     6001 cctctttagt tttatccaat ggaagaaaat caagaactcg taaacagaat atttctaatt
     6061 cgtaaccaac gtgtgatgtt ggacagtgat ttagctgaaa tttatggagt agaaacgcgc
     6121 aggttaaatg aacaatttaa acgaaatgaa atacgctttc ctaaagattt tgcctttagg
     6181 ttaacagctg ctgaatggac gaatttaaaa tcacaaatgt tagtcaactc aacagaatct
     6241 attgattcta ttattgaagc tgatgacaat tcatctcaag attggggtgg acgaagaaaa
     6301 atgcccttag tatttacaga acatggggca gtaatgttag ccagtattct aagcagtgag
     6361 aaagctgttg aagctagcat ttttgtagtg cgaactttta tacgctttcg ccacatgata
     6421 agtacgcaag atcatttggc cacgaaaatt cttcagcttg aggagaaatt tgatggacaa
     6481 ttcaatattg tatttgatgc attacgtgat ttactcgaac atcctccttt acctaaaata
     6541 cgtcaacgaa ttggatttaa gaaagatgag gtagggtaaa cctaatggta ttgactgatt
     6601 attaagcatt aagaactttc tttagaacat taatcacatt ttaccaacat tcccgttttc
     6661 tcatcttcaa accatttact tttgctctcc ggttaaaccg gtccccaatg cccaaaaaat
     6721 cagaaatccg ctccgttcta ataatcggta gcggtccgat tatcatcggt caagcatgtg
     6781 aatttgatta ttcaggttct caagcagctc gttcacttcg tgaagaagga attgaagttt
     6841 ccctgataaa cagtaatcca gctactataa tgaccgacaa agtttctgcc gatcatgtgt
     6901 atttgctgcc tttgactgtt gcttcgattg tcaaaattct ggaagaaagg aagattgatg
     6961 ctgtactacc tacaatgggc ggacaaacag cattgaactt ggctaaaaaa gccgacgaga
     7021 tggggatttg ggctaaatat ggggtgaaga ttattggggt tgaaattgat gctatcgaaa
     7081 cgactgagaa tcgtgaagca tttcgtcaac tatgcgtcga tcttaatgtt ggagtaccgc
     7141 catctcaaat agctaattcc tttttagaag gtaaagaaat agctcaacga attggatatc
     7201 cattagtcat tcgtccatcc tatacattag gtggaacagg tggtgcattt gttcatcgta
     7261 aagaagattt tgatgagtta ttgaaccgag gcttgcatgc ttctcccact catgaagtat
     7321 taattgaaaa ggcccttttt ggctggaaag aatttgaatt agaattatta cgcgataaca
     7381 atcagaacat catcataata tgttctattg agaatttcga tccaatggga attcatactg
     7441 gagattccat tactgttgct ccagccatga cattaagcga tactgcctat caacatatgc
     7501 gtgatatggc aatcaaactt atgcgtcata ttggaacttt tgcaggagga tgtaatgttc
     7561 agtttgccat gaatcctgaa aatgaagata tcatcgctat tgaaattaat ccaagagttt
     7621 ctcgttcatc agctctagct tcaaaagcta caggttatcc aattgctaaa atagcggcta
     7681 agcttgcaat cggttataat ctcgatgaat taagcaatca aattacaggt acaacatccg
     7741 cttattttga accaacactt gactacgtta ttgtaaaaat acctcggtgg aatttcgaca
     7801 agtttaaagg tgccgataaa tctcttggcc ttcaaatgaa gtcagtgggt gaagtaatgg
     7861 gaattggtag aaacttccag ga
//
LOCUS       DAOOQW010000101         7879 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_11377_length_7879_cov_5.961784, whole genome shotgun sequence.
ACCESSION   DAOOQW010000101 DAOOQW010000000
VERSION     DAOOQW010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 7879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7879
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11377_length_7879_cov_5.961784"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..1766)
                     /locus_tag="PK281_06960"
     CDS             complement(84..1766)
                     /locus_tag="PK281_06960"
                     /inference="COORDINATES: protein motif:HMM:NF025627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004062 - aryl sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aryl-sulfate sulfotransferase"
                     /protein_id="HOY49123.1"
                     /translation="MNLNRTLLFTLFFAFIFPLTAQQWGNYTLVSLQGQSTAKLIDTL
                     NATYHTYTPGSGSSAYSAFILPGGEYIRSISVSNPTFTGGGMTGGIQKVDWNNNVTWQ
                     YNYSTPSYSMHHDFCPMPNGNVLLIAYELKTASEVTQAGSSTSHIMWPDKIVEVQPNG
                     ATGGTVVWEWHAWDHLVQSVDATKDNFGVVAEHPELLNINYNNNANTKDWMHANGIDY
                     NPLLDQIILSSHNLNEFYVIDHSTTTVEAASHQGGNSGHGGDILYRWGNPAAYGASGT
                     NIFHTVHDAHWIPENYPNAGYMVGYNNNGISNNQSSVEFVAPPYAENYSYSHTAGSAF
                     SPSTYTLRHACSGHNNNMGSSQSLPNGNLIVCVAQSGLIYEVNAAGTQLWSYTASGTM
                     AQAFRYDNCYLTTPRPTVPTISLSGNVLTSSSATSYQWYLNGSLIPGATSQSYTPTQN
                     GVYLVKVGEPNACQFNYSVGFRYSTILTTSNPSKSELSIYPNPANDIIQIAGIPSGAS
                     VLVSLFNMTGQLVDLKSNVTNLDVSFLPDGVYVIRATVNGTPTYSQKLLLAH"
     gene            2204..2827
                     /locus_tag="PK281_06965"
     CDS             2204..2827
                     /locus_tag="PK281_06965"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOY49124.1"
                     /translation="MNEKLEHIIQQARSIFMRCGIRSVTMDDVCREMRISKKTLYQHV
                     HDKTDLVNQTMRSEIAKDEVEVSTLISLGLSAIDEMFEITRIVSEKIRNIHPSILFDM
                     QKYYPEAWEMMRIHRNDFIERVIQKNIQKGQDEGVYRSNMNAIIVSRLFAIKIDVLSD
                     NHFFEGTGLSMTQVNFEHLTYHLYGISTPKGIYYLEQKLSNLNPQSL"
     gene            2824..4170
                     /locus_tag="PK281_06970"
     CDS             2824..4170
                     /locus_tag="PK281_06970"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOY49125.1"
                     /translation="MKNKVLLLLLILSAGLHAQTNDQGSFTLPQAIDFALKNGYSVKN
                     AATDIEIAKKKVAEIRGIGIPQLKTEASFQDFVEVPVSIISANAFNPLAPPGTYLRIP
                     FGVKYNASIGYTASWLAFSGEYIVGLQASKTYVDISKANLRKSEIEVKESVTKAYNLV
                     LILKENKRILSENIASLEQSIEQTSAYNKEGFVEEMDVDRLRLLKNNLSTTLTTLEQQ
                     TIIAEKLLKFQMGYDVNAAITLTETLDNLISNAKSGVDVEPKFDPANNIDRILIGKAL
                     NLQKLEVKRQKANYLPTLSTFYTWKESRITNEFDQLSNNLFRVPGGTILGVNLSMPVF
                     QGMSQSARVKQAKLNLKKLEVQQIQAEQGIALQSSQSWTAYATALSGLRNTQESVGLA
                     TRIRDRAKIKYQEGVGSSLEVIQAENDLLSSQSNYINAVQQLLETRVTLDKNLNKF"
     gene            4188..5318
                     /locus_tag="PK281_06975"
     CDS             4188..5318
                     /locus_tag="PK281_06975"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOY49126.1"
                     /translation="MKKLLYISITLLVAACSSGNDLEKKKAQLAEYQAQHDELKVKIA
                     ELEKEIALLDTAAEVRKPKLVTLTPIVSGEFNHFIDVQGTVDSEENIAIQPGMPGLVT
                     RVTVHEGDMVSAGQILAEVDNKTIRESMAQLQTNLDFAKTAFEKQQRLWNQKIGTEIQ
                     FLQSKTQYESLQKSMATLQAQLDMSRIKSPISGVVDAVNVKVGEFAAPGINGAFRVVN
                     FSKMKVKAKVADSYIGKVKAGNPVRIVLSDINDTIQGKISYVSKVVNPMTRTFDIEIA
                     LGVTNSDVRPNMLATLNINDENLPGAMSVLSNLVQKDASGNYYMMVAEGAGDKMTAHK
                     KIVSTGISYGDRIVITEGLNGSEKLILSGYQEVVDGQLISLK"
     gene            5344..>7879
                     /locus_tag="PK281_06980"
     CDS             5344..>7879
                     /locus_tag="PK281_06980"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOY49127.1"
                     /translation="MEKEHIKYFKPTNWAINNSTAIYIFTVLISLAGIIIFQRIPKEK
                     FPDIVVPTIFVSTIYPGASPEDIENLITKPVEKEVKSVSGIKKVTSNSIQDFSLVVVE
                     FNTGIDVDVAKQKISNAVDKAKKDLPSDMKTDPSVQEVNFSEFPIMNINVNGEFPLDQ
                     LKRYAEDLKDKIETLPEITRVDIIGALTREVQVNVDLYRMQALGITFTDIERAVASEN
                     VNISGGEIRIDELRRTLRVRGEFSDVSQIGNILIRSARGNLAYLKDIAEVKDGYKDKQ
                     DFARLEGKPVITMNVIKRSGENLIDAADKIKVIIDEYKATKFPKGLDVTLTGDTSIET
                     KTQLNDLVNTVIIGFVLVVIILMFFMGLQSAFFVGLAVPLSVLVALLFMPSLGFSMNV
                     IVLFSFLLALGIIVDDAIVVIENTHRIFHKYDFDIKTAAKAAAGEVFVPVLAGTLTTI
                     APFFPLLFWPGIVGDFMGYLPITLILTLFASLFVAFIMNSVFAVSFMKRDEHDHKTSA
                     KELVRPAIIFGAVALLCHAAGMTAVGNVSFLLLALTPLYLYILRPMTVRFQMDIWPQF
                     VAAYKRAIKVVLIGRRPILMLVGVVVLFILSIVTFVASNPKIEFFPNGQPNFAYVYLE
                     LPIGTDAEVTDSVTRIVEKRVFEVIGENNPNVESVISNVGIGAGDPRNPDRVATPHKG
                     KVSVAFVDFSERGDFSTLECLEAIRNNMKGIPGVRITVDKEANGPPTGKPVNVEISGD
                     DFDQLQLIETKMRAAISKAKIQGIEDLQSDLQRNKPEIIVTIDPDRALSLGMSKAQIA
                     MELRTALFGKEISKFRDADDDAEITLRLASNYRDKVDDLLNMQIAFM"
BASE COUNT         2498 a         1423 c         1719 g         2239 t
ORIGIN      
        1 tacttttttc gcaaatgagg agagcgaaca aagaagaaat agcgagagca gaatatgttt
       61 catgacaaag tttgagatca aaatcaatga gctagaagga gtttttgaga gtaggtaggt
      121 gtaccattta cagtagctcg aataacataa acaccatcag gcaagaaaga aacatcaagg
      181 ttcgtaacat tacttttaag atcgactagt tgaccagtca tattgaacag agatacaaga
      241 acagatgctc cagaaggaat tccagcaatt tggataatat cgtttgccgg atttgggtaa
      301 atgcttaatt ctgattttga aggattggaa gtagttagta tagttgaata ccgaaatcca
      361 actgagtaat tgaattggca agcgtttggt tcaccgactt ttacgagata tacaccattt
      421 tgagttggtg tgtatgattg agaagtagcg ccgggaatta gggagccatt taaataccat
      481 tgatagctag ttgctgaaga gctcgttaat acatttccag aaagtgaaat tgtaggaact
      541 gtaggtcgag gagttgttaa atagcagttg tcgtagcgaa atgcttgagc cattgtgcct
      601 gaagcagtat aagaccaaag ttgagttcct gcagcattta cctcataaat caaccctgat
      661 tgagcaacac aaactatcaa gtttccgttg ggtagcgact gagagcttcc catgttgtta
      721 ttatgtccgc tacatgcatg tcgaagtgtg taggttgagg ggctaaaagc agaaccagca
      781 gtatgagagt agctgtaatt ttcggcataa ggaggagcta caaattcgac agatgattga
      841 ttgtttgaaa ttccgttatt gttgtaacca accatgtatc ctgcatttgg ataattttct
      901 gggatccaat gggcatcatg aacagtatga aagatattgg ttccagaagc accataagca
      961 gcaggatttc cccaacgata aagaatatca ccaccatgac ctgaatttcc accttgatgg
     1021 gaagcggctt ctactgtagt agtactgtga tcgattacat agaattcatt gaggttgtgc
     1081 gagctcagga tgatttgatc aagtaaagga ttatagtcaa tcccatttgc atgcatccaa
     1141 tcttttgtat tcgcgttatt gttatagttg atatttaata attcgggatg ctcagccacc
     1201 acaccgaagt tgtctttagt agcatcaaca ctttgcacga gatgatccca agcatgccat
     1261 tcccaaacta cagttccgcc cgttgcgcca ttcggttgta cttcgacaat tttatctggc
     1321 cacataatat ggctagttga acttcctgct tgtgtaacct cagaggcagt tttaagttcg
     1381 taggcaatta gtaggacatt tccatttggc atcggacaga agtcatgatg catactgtag
     1441 gaaggagtag aatagttgta ttgccaagta acgttattgt tccagtcaac tttttggata
     1501 ccaccagtca ttccgccacc agtaaacgtt gggtttgaaa cagaaattga gcgaatgtat
     1561 tctccgccag gcaaaatgaa agctgaataa gcagaagaac cggatccggg agtataagta
     1621 tgataagttg cattaagcgt atcaatcaat ttggctgttg attgaccttg caaggaaaca
     1681 agtgtgtaat taccccattg ttgggctgta agcggaaaaa taaaagcaaa gaaaagggta
     1741 aagagaagtg ttctatttag attcatagaa agaagttgtc agaccgccta tgaataagaa
     1801 cgaacttgaa ggtttaattt aatgttaaaa atatgttacc taaaatgaga ggaataagtg
     1861 gcgtcggtaa atccagaaag atgaaagacg cttctcatca aaccatccgg gatagtctca
     1921 accaaaaaac acgacccatg cgactaccga acgccactca aagattaaaa gtagagaaag
     1981 caatatatac tttcaagact tggcaaaaat aatctctttt aaaatgaaaa aacagttctt
     2041 taactggaat attgttttta ccaaatttta aagtggcgga actgcgttgt tctagcttac
     2101 tcatttactg tgcattataa ctgaatacgc ccaaaagaaa cacaggaaac atttttattg
     2161 caatttgttt ccaaggttcc caattcgttt tacctttgcg gcgatgaacg aaaagcttga
     2221 acatatcatt caacaagcac gatctatttt catgcgttgc ggaattcgta gtgttactat
     2281 ggatgatgtt tgccgggaga tgcggatttc gaaaaagaca ctataccaac atgtgcatga
     2341 caagaccgat ttggtgaatc aaaccatgcg ttctgagatt gccaaagatg aagtagaagt
     2401 gagcacgctt atttcactcg gattaagcgc aatagatgag atgtttgaaa tcacacggat
     2461 tgtttcagag aaaatcagga acattcaccc atccattctg ttcgacatgc agaaatatta
     2521 tccagaagct tgggaaatga tgcgtataca tcggaatgat ttcatcgaac gggtgattca
     2581 aaaaaacata caaaaaggcc aagatgaagg agtttaccgc agcaatatga atgcgataat
     2641 tgtatcccga ttgtttgcta ttaaaattga cgtactctct gataatcact tctttgaagg
     2701 aacaggattg tccatgacac aagtgaattt tgaacacttg acctatcatt tatatggcat
     2761 atcaaccccc aaaggcattt actacctaga acaaaaactt tcaaatctta atccccaatc
     2821 cttatgaaaa acaaagtgct gctgctcttg ctgatactct cagccggact tcatgcgcaa
     2881 acgaacgacc aaggaagttt tacacttcca caagcgatcg attttgcctt gaagaatgga
     2941 tacagtgtca agaatgctgc aacggatatt gaaatagcca aaaagaaagt ggctgaaatc
     3001 cgtggaattg gtattccaca gttgaaaaca gaagcttctt tccaagattt tgttgaagta
     3061 cctgtcagca ttatttctgc aaacgctttc aatccgcttg ctccaccagg aacttatttg
     3121 cgaattcctt ttggtgtgaa atacaatgct agtataggct ataccgcttc atggctggct
     3181 tttagtggag aatacatcgt tggtttacaa gcatctaaaa catacgtaga tatttcaaaa
     3241 gccaatttga ggaaaagtga aatcgaagta aaagaaagcg ttaccaaggc ttataacctg
     3301 gtgttaattc tcaaagagaa caaacgaatt ctttcggaaa acatcgcttc acttgaacaa
     3361 tcgattgaac aaacatcagc ctataacaaa gaaggatttg tggaagagat ggatgtagat
     3421 cgtttgcgct tgctgaaaaa caacttaagt acaacgctta ccacattgga acagcaaacc
     3481 atcattgctg aaaaactgct caaattccaa atgggttatg atgtgaatgc ggcaattaca
     3541 ctaaccgaaa cgctcgacaa cttaatatca aatgctaaat cgggagtaga tgtggaacct
     3601 aaatttgatc cggctaacaa catcgaccga atattaattg gcaaagcact taaccttcaa
     3661 aaactggaag ttaaacgcca aaaagccaat tacctgccta cactgagcac attctatact
     3721 tggaaagaaa gtcggattac taatgaattc gatcaactca gcaacaattt atttcgagtt
     3781 cccggcggta caatcttagg tgtaaacttg agtatgcctg ttttccaagg aatgagccag
     3841 tcggcacgtg ttaaacaagc taaactgaat cttaagaagt tagaagttca gcaaattcaa
     3901 gcagaacaag gcattgcatt acaatcgtct caaagttgga ctgcatatgc aacagcactt
     3961 agtggcttaa gaaacacaca agaatcggtt ggattagcaa cacgtattcg tgatcgagca
     4021 aaaataaaat atcaagaagg agtgggatcg agcttagagg ttatacaagc cgaaaatgat
     4081 ctgctttctt ctcaatcaaa ttacatcaac gcagttcagc aattgttgga gacacgcgta
     4141 accttggata aaaacctgaa caaattctga tacccgaatt cgaacaaatg aaaaagctcc
     4201 tttatatctc cataacgctg ttagttgcag catgttcatc cggaaatgat ctggaaaaaa
     4261 agaaagctca gcttgccgaa tatcaagctc agcatgatga attaaaagta aagattgcag
     4321 aactcgaaaa agaaattgct ctactcgata ctgcagcaga agtgcgtaaa cctaaactcg
     4381 tcaccctcac tcctatcgtt tctggtgagt ttaaccactt tattgatgtt caaggaacag
     4441 tagattcaga agagaatatc gctattcaac ctggaatgcc aggattagtt acccgtgtga
     4501 ctgttcacga aggtgacatg gtttcggccg gacaaatttt agctgaggta gataacaaaa
     4561 ccattcgtga gagcatggct caattgcaaa ccaatttaga ttttgctaag actgctttcg
     4621 agaaacaaca acgcctttgg aaccaaaaga ttggtacaga aattcaattt ttacaaagca
     4681 aaacacagta tgaatcgctc cagaaaagca tggcaacttt gcaagcgcag ctggatatga
     4741 gccgtattaa atcaccaatt tcgggtgtgg ttgatgcagt gaatgtgaaa gtaggcgagt
     4801 ttgcagcacc aggtattaac ggagctttcc gcgtggtaaa ttttagcaaa atgaaagtaa
     4861 aagctaaagt ggctgattca tacatcggta aagtgaaggc tggaaatcct gttcgtattg
     4921 ttttgtcaga catcaacgat accatccaag gtaaaatcag ctatgtgagc aaagtcgtta
     4981 atccaatgac acgcacattt gatatcgaaa ttgctttggg tgtgaccaat tcagatgttc
     5041 gtccgaatat gttggcaaca ttaaatatca acgatgaaaa tctaccggga gcaatgagtg
     5101 tattgtcgaa tctagttcag aaagacgcat cgggtaacta ttacatgatg gtggctgaag
     5161 gagctggaga taagatgact gctcataaga aaattgtttc tacaggtatt tcctatggtg
     5221 atcgcattgt gattactgaa ggacttaatg gaagtgaaaa actgattcta tcgggctatc
     5281 aggaagttgt tgacggacaa ttaatctccc ttaaataatt cccggattta atcccaaccc
     5341 atcatggaaa aggaacatat aaagtatttc aaacccacca actgggcgat taataacagt
     5401 accgcgattt acattttcac ggtattgatt tccctagctg gtatcatcat attccaacgg
     5461 attccgaaag agaaatttcc ggatatcgtg gttccgacca ttttcgtttc gacgatttat
     5521 ccaggagcta gtccagagga tattgaaaac ctgattacca agcctgttga gaaagaggtg
     5581 aaatcggtat cgggtattaa gaaagttaca tcgaattcga ttcaggattt ttcgctcgta
     5641 gtagtggaat ttaatactgg aattgatgtg gatgtggcta agcaaaagat ttcgaacgcg
     5701 gtagataaag cgaagaaaga tttgcctagt gatatgaaaa cagatccgag tgttcaggag
     5761 gtaaacttct ctgagttccc gatcatgaat atcaacgtaa atggggagtt tccgcttgat
     5821 cagttgaagc gttatgcgga agatttgaag gataaaatcg agactctacc tgaaattact
     5881 cgagtagaca ttattggagc attaacacgc gaagtgcaag tgaatgttga cttgtatcgt
     5941 atgcaggctc ttggaataac ctttacagat attgaacgag ctgttgcaag tgaaaacgtc
     6001 aatatttcgg gtggtgaaat tcgaatagat gagttgcgca gaacccttcg tgtacgaggt
     6061 gaatttagtg atgtgagtca aattggtaac attctaatcc gttctgcacg aggtaattta
     6121 gcgtatttga aagatatcgc tgaggtgaaa gatggttata aagacaaaca ggactttgcc
     6181 cgtttggaag gtaagcctgt tataaccatg aatgttatta agcgttcggg tgaaaacctc
     6241 attgatgcag ccgataagat taaagtgata attgatgaat ataaagcaac taaattccct
     6301 aaaggactgg atgttacctt aacaggagat acttcgattg aaaccaaaac ccagttgaac
     6361 gacttggtta ataccgtgat cattgggttt gttttggttg ttattattct gatgttcttt
     6421 atgggcttgc agagtgcgtt tttcgtagga ttggcggttc ctttatcggt tttggtggct
     6481 ttattattta tgccttcgtt aggattctcg atgaacgtaa ttgtgctttt ttcgttcttg
     6541 ttggcactgg gaattattgt ggatgatgcc attgtggtaa ttgaaaatac ccacaggata
     6601 ttccataagt acgatttcga tataaagacg gctgccaaag ctgctgcagg tgaggtgttt
     6661 gtgcctgtgt tggctggtac attaacgacc attgctccat tcttcccatt gcttttctgg
     6721 ccgggaattg tgggtgattt catgggttat ttaccaatta ccttgattct aaccctattt
     6781 gcttctttgt ttgtggcatt tatcatgaac tcggtgtttg cagtgagttt catgaaacgc
     6841 gatgagcatg atcataaaac atcagcaaaa gaactggttc gtcctgcaat catttttggt
     6901 gcggtggctt tattatgtca tgcagctgga atgaccgctg ttggtaatgt gagtttcttg
     6961 ctattagcat taactccact ttacctatac attttgcgtc cgatgacggt gagatttcaa
     7021 atggacattt ggccacaatt tgttgcagca tacaaacgtg ccattaaagt tgttttgatt
     7081 ggtcgtcgtc ctatattgat gcttgttggc gtggttgtat tgttcatttt atcgattgtc
     7141 acattcgtag catctaatcc taaaattgag ttctttccaa acggacagcc caattttgca
     7201 tacgtatatc ttgaactacc cattggaaca gacgcagagg taaccgattc ggtaacacgc
     7261 atcgtggaga agcgcgtttt tgaggtgatt ggagaaaata atccaaatgt ggagtcggta
     7321 atttcaaatg ttggaattgg tgcaggagat ccacgaaacc ctgatcgggt agcaacacca
     7381 cacaaaggaa aagtatctgt tgcatttgtt gattttagcg agcggggcga cttttctaca
     7441 cttgagtgtt tggaagcaat ccgaaacaat atgaaaggca ttccgggtgt tcgaatcacg
     7501 gtagataaag aagccaatgg tccacctaca gggaaaccag taaacgttga gattagcggt
     7561 gatgattttg atcaattaca gttgattgaa accaaaatgc gcgcagctat ttcgaaagcg
     7621 aaaattcaag gtattgagga tttgcaatcg gatttacagc gaaacaaacc agagattatc
     7681 gtgacgattg atcctgatcg tgctttgtct ttaggtatga gtaaagctca aattgcgatg
     7741 gaacttcgta cagcattgtt tgggaaagag atttccaaat ttcgcgatgc agatgatgat
     7801 gctgaaatca cactacgatt ggcttctaac taccgcgata aagtggacga tttgttaaac
     7861 atgcaaattg ctttcatgg
//
LOCUS       DAOOQW010000102         7875 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_11388_length_7875_cov_4.846164, whole genome shotgun sequence.
ACCESSION   DAOOQW010000102 DAOOQW010000000
VERSION     DAOOQW010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 7875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7875
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11388_length_7875_cov_4.846164"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="PK281_06985"
     CDS             complement(<1..269)
                     /locus_tag="PK281_06985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49128.1"
                     /translation="MNSRLTSLLIGVLFLFLSSTIYGQTYIMGETSGVITTCSGIIEN
                     PGGFGSYTINQDVTQTICSGNGQCIQLTFTEFGTEACCDQLTVYDG"
     gene            complement(630..3533)
                     /locus_tag="PK281_06990"
     CDS             complement(630..3533)
                     /locus_tag="PK281_06990"
                     /inference="COORDINATES: protein motif:HMM:NF012646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY49129.1"
                     /translation="MNARLYSLLIWIVFLFLSTTIYGQTYIMGESSGTITTCSGILEN
                     PGGFGSYADGLDVFQTICTGNGQCIQLTFTDFGTEGCCDRLTIYDGNSEFSYEIIEFA
                     GTSNPGIVTSTDASEGCLTLKFFTDGSVGSTGFRANISCINCPTNFILGQGLDSLSTC
                     SGIILDPGGSLNYEDSLNVSQTFCSSDSGQCLTLSFNSFNTELNFDVLSIYDGPNTNS
                     ELFGHFSGSEIPEPISSSSNSGGCLTLLFNTNNSINFNGFHAYLNCGNCSYPDLILGH
                     GGDTIYTCGGRLLDPGSLNDYNSNQNITQTICSETPGQCVSLSFNSFNTEAVYDRLSV
                     YDGPSNTSNLISIFSGNSIPPIISSSTNSEGCLTLVFTSNGSVNLEGFSADINCGICD
                     DPFINNPLYCQISQALCLGQGENNYVASTQLDSEFGSPGPIGCLGATPNPAWFYFQVQ
                     DSGPIDIEITSGFDVDFICWGPFNQAQWSNGVCSMVLDPVWANDNLNSVSCSYSASPI
                     EHCDISNTQSGEYYVLLVSNISNKSSIINIQQIDGIGTTNCSSLCDHTINTLVTECDS
                     TNNTYSIHCTIYLPHPPLSGNVEIINSSGGILSFEAPFPDSLNFSFDHLASDGLQHSI
                     EVSFSDQPNCTNSFFYTAPLACNACAVSASLLDSVSCTGFPIHLQASELPSVTYSWIG
                     PNGFTSSEQNPIIANAELNMNGVYQLDVYNPIDSCHSIALVNVLVHQFPVDASMTGNE
                     PICSGGSIILDVPDYSNAIYTWTKSDSTLISSSSSTYYLQPAILQDMGIYSCTINIEG
                     CINTSQDEDVIIYQNPLIPYFDFNPLTNELYAMQSAGNFQWFLNNVPIIGQTQYHIHL
                     EQQGNYSVTVSNDFGCVISSENFYFDPTGIPELPISRLALQPNPAKAYIQVLVPELGV
                     LEVFDLAGKRLKEYKLQLGVNTISVNELAEGIYLFQFHSNSGVRLAKVIVEHN"
     gene            complement(3719..5458)
                     /locus_tag="PK281_06995"
     CDS             complement(3719..5458)
                     /locus_tag="PK281_06995"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HOY49130.1"
                     /translation="MKTLLFWLFNVGIVVNIFGQCPFNPTITPSTLQLCPNSSDTLWT
                     ETATTYQWYRGGNPVANSNHPYLVVNSATDGGQNFIVASTINGCTEASPAAAVSVYNP
                     PAIALSVGENLPLTGCEGDPRQLIINDPYNENILWFRNGVQLNGQNNDTLNVNQSGSY
                     SVIAFTDQCPLYSQTSAATVFNFTASQTPVIQFNSGNLSLSTSTIAGSYQWYLDSNPI
                     TNATSSTYLPQANGLFTVEAIFSGTCSRYSTPYNYTSFVQECTQDPIVTPSDLILCPG
                     ATDTLFTQAADSYQWYREGVALIGATDSFLVVDAFSAAGAQYSVETTVNGCTEMSPSV
                     LVDGWVFLPIYVISEFPNGETLCEGDSVILTVGNPFTNSISWFKNGSLIEGQSESSYT
                     VTTNGTYTVTAFTGTCPDYGETSLPLDYSFGVAPIPNINFFSVSNSLGTNANAVTYVW
                     NLDGNPISGATNQVYNLGEIGGTYTLTCTYSNGCSNTSLPFIYDPTGIKGVKTLALEI
                     GPNPAKSEFFVELPEDGTLEIWNAAGSLTGSYNLKKGKHVLSTEQRAAGIYMLKFSSS
                     KSVWTARLQLTGL"
     gene            complement(5545..6312)
                     /gene="rlmB"
                     /locus_tag="PK281_07000"
     CDS             complement(5545..6312)
                     /gene="rlmB"
                     /locus_tag="PK281_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018676145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HOY49131.1"
                     /translation="MNKPFHHKPAQDTSEMIFGMFPVLEALRSSADVDKVIMQTGLRS
                     PQLHELRELLDELEIPLQLVPREKLDRMTRANHQGVIGFISPVTFQPIEEVLQRVFEA
                     GRDPFLLILDRVTDVRNFGAICRTAECAGIDAVIIPSRGSARVGGDAMKTSAGALMNL
                     PVCRSTNLKDTLDYLKNSGIRLVGCTEKTESPLYATKLEGPLGIIMGSEEDGISPEYL
                     KRCDERVKIPMFGKTGSLNVSVATALVVYEVVRQRIG"
     gene            complement(6305..7543)
                     /locus_tag="PK281_07005"
     CDS             complement(6305..7543)
                     /locus_tag="PK281_07005"
                     /inference="COORDINATES: protein motif:HMM:NF040439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HOY49132.1"
                     /translation="MPKTRLSLFLLVLLAGCSANRNPFSQNRYLDYRFPQPGPPMELQ
                     YWAISTDSVAIRLRFLNADLGLMPDADNLEPKRRLLIRASFYPEFKRRKPIVSQPLVL
                     ESGLSDTAIFTGAELHLPMLTGTDGLLELDIRDLQKNRSRFKYVNLSRLKEGNALDFQ
                     VFEKGNPSIHTWVRIGNPCNIRYVDKLEQRFIVEHYVAEFPLPAPPFAVSSELKPFPD
                     AFLKESISRDELLDFRFEKQEFLRISSGNSPTSGLGLLVVGSNFPGLSNDSERFRCMV
                     YLMQKEEIKQFSGVSDFHLWKFLKLDKAHSEAVEKMWLDRIRHANLLFTSFVPGWRTD
                     RGMIYTIFGPPSSVYNNGRAEVWNYNPKSGSTPLSFRFERISQAYYQDEYRLERKATY
                     ANVWYKAVESWRNGNAFSYE"
     gene            complement(7524..>7875)
                     /locus_tag="PK281_07010"
     CDS             complement(7524..>7875)
                     /locus_tag="PK281_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015363875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HOY49133.1"
                     /translation="FDKNIVVVSPTTLLATLRTIASIWRQEYQNQNAQEIADQGGKLY
                     DKFVAFSVDLIKLGNQMDTAKKTYEESMKKLTDGSGNLITRVEKLKKLGARSSKVLPT
                     ALLNRADPDAEDEA"
BASE COUNT         2614 a         1562 c         1405 g         2294 t
ORIGIN      
        1 ccatcataaa cagttaattg atcacaacaa gcctctgttc caaattcagt aaaggttagt
       61 tgaatacatt gtccatttcc cgaacaaatc gtttgtgtta catcctgatt aatagtgtaa
      121 gacccaaatc ctccggggtt ttcaattatt ccagagcagg ttgttattac tccacttgtt
      181 tctcccatga tataggtttg accataaatg gtggaggata aaaacagaaa taaaacccca
      241 attaataggc tagttaaacg tgaattcata ggcttttaaa ttaaaatata tagaactata
      301 ggggtaggat taatgctaaa atatcaagca ttaacggtat aattctatac aagtatagtt
      361 aaatattgaa ttcaattcag cgactgttta aatgatttgt tgtgattgaa tttactaggt
      421 ctttttctgt taaggcggct aaaaatatcc aatttcttgt ctgtcgtcgt atatgtgagc
      481 attactaaat tcaattctgg gacctaataa tttctctaca tattaattca cggtcaggtt
      541 cgtatcaacg gtatattgag cgtttattat cgttggaaaa attaaaaagt actcttaaaa
      601 gtaaaatgag ccttttcata attcacgaat taattatgct caacaataac tttcgctaac
      661 cgaacacctg aatttgagtg gaattgaaaa agataaattc cttcagctaa ttcattcaca
      721 gaaattgtgt tcacgcctaa ttgtaattta tattctttta aacgttttcc tgctaaatca
      781 aacacttcta aaacgcccaa ttctggcact aacacttgaa tataagcctt tgcgggattg
      841 ggctgcagtg ctaatctaga aatgggcaat tctggaattc cagtaggatc aaaataaaaa
      901 ttctcggatg aaataacaca cccaaaatcg ttcgacacag ttacactgta attaccttgt
      961 tgttcgagat gtatatgata ttgtgtttgt ccaataattg ggacattatt taaaaaccac
     1021 tgaaaattcc ctgctgattg catcgcgtat aactcatttg tcaatggatt aaaatcaaaa
     1081 tatggaatta atggattttg ataaattatt acgtcttcgt cttgcgaagt attgatacat
     1141 ccttcaatat taattgtaca cgaataaatt cccatatcct gcaaaattgc tggttgcaaa
     1201 taataagttg aactagagct ggagattaaa gtactatctg atttagtcca agtataaatt
     1261 gcatttgaat aatcgggaac atccaagatg attgagccgc ccgaacaaat aggctcatta
     1321 ccagtcatac ttgcatcaac tggaaattga tgaacaagca cattcaccaa agcaatactg
     1381 tgacaactat caatgggatt ataaacgtcc aattgataaa ctccattcat atttaattct
     1441 gcattggcta ttattggatt ttgttcagaa gatgtaaatc cattggggcc aatccatgaa
     1501 taagttacac taggtaattc agaggcttgc aagtgaatag gaaagcctgt acaagaaact
     1561 gaatctagca aggatgctga aacagcacag gcattgcatg ctaaaggagc agtataaaag
     1621 aatgaattag tacaattggg ttgatccgaa aaggaaactt caatggaatg ttgtaaacca
     1681 tcggaagcaa ggtgatcaaa actaaaattt aaggaatcag gaaatggagc ttcgaaactt
     1741 aagattcctc cagaagagtt gattatttcc acatttcccg aaagcggcgg atgcggcaaa
     1801 taaatcgtgc aatgaattga ataggtattg tttgtggaat cgcattctgt tactagtgtg
     1861 tttattgtat gatcacaaag tgagctacaa ttagtagttc caataccgtc gatttgttga
     1921 atattaatta ttgaagattt attagagata tttgatacta aaagcacata atactctcct
     1981 gattgtgtat ttgaaatatc acaatgttct attggactag ctgaataact gcaagaaaca
     2041 ctattcaaat tatcatttgc ccatactgga tctaatacca ttgaacaaac accattactc
     2101 cattgtgctt gattgaaggg tccccaacaa ataaaatcta catcaaaacc ggaggttatt
     2161 tctatgtcaa ttggacctga atcttgaacc tgaaaataaa accaagctgg attgggagtt
     2221 gcaccgagac atccaattgg acctgggctg ccaaattcag aatcaagttg agttgaagca
     2281 acgtaattat tttcaccttg acctagacaa agtgcctgag atatctgaca atataatgga
     2341 ttattaatga atggatcatc acaaattcca caattgatat cagcagaaaa cccttctaga
     2401 ttaacagaac catttgatgt aaaaactaat gttaaacagc cttccgaatt tgtagaactt
     2461 gaaatgatag gtggaataga atttcctgaa aaaatagata ttaaattact cgtattactt
     2521 gggccatcat aaacggatag ccgatcgtat acagcttctg tattgaaact gttaaaactt
     2581 aatgaaacac attgtcctgg tgtttcggaa caaattgttt gggtgatatt ttgatttgaa
     2641 ttgtaatcgt tcaatgaacc tgggtctaat aaacgtccgc cacaagtata aattgtatca
     2701 cctccatgtc ctagtattag atcagggtaa gaacaatttc cacaattcaa gtatgcatga
     2761 aaaccattaa aattaattga attattagtg ttaaacaaca aagtcaaaca tcctccagaa
     2821 ttgctggatg acgatattgg ttcaggaatt tccgatccag aaaaatgccc aaataattcc
     2881 gaatttgtat ttggtccatc gtatatggat agtacatcaa aatttaattc cgtattgaaa
     2941 ctgttaaatg ataatgttag acattgtcct gaatctgaag aacaaaatgt ttgagataca
     3001 tttaaactat cctcataatt taaacttcca cctggatcta aaataattcc tgaacaagta
     3061 gacaatgagt ccagcccttg tcctaaaata aaatttgtgg gacaattaat acatgaaata
     3121 tttgctctaa agcctgtact acctactgat ccatctgtga agaattttaa agttaaacat
     3181 ccttctgagg catctgttga tgtgactatt cctggatttg aggtacctgc aaattcaatt
     3241 atttcatatg aaaattcact atttccatca taaattgtca atcgatcaca acaaccttct
     3301 gttccaaaat ctgtaaaggt tagttgaata cattgcccat tcccagtaca aatcgtttga
     3361 aatacatcaa gaccatctgc ataggaacca aaaccacctg gattttcaag aattccagaa
     3421 caagtcgtta ttgtcccact tgattctccc ataatatagg tttgaccata aatagtagtg
     3481 gataaaaaca gaaaaacaat ccaaataagt agactatata atcgtgcatt cataggcttt
     3541 ttaataaatt actaaaacaa atgagataga attaatacct aataattaag cattaacaga
     3601 ttaatcccat acaagtataa ttaacctaag taatcaattc agcgacagtt tatacgattt
     3661 gtcgcaaaat aatcaactag ctatcgcaat agttaaatta aaaaatgatg gagcaattct
     3721 acaaacccgt taattgcaaa cgagcagtcc agacagattt acttgaagaa aacttaagca
     3781 tataaattcc agcagcacgt tgttcagtgc ttaatacatg ctttcctttc ttcaaattat
     3841 aagaacctgt caacgaacca gcagcattcc aaatctcaag cgttccatcc tcagggagct
     3901 caacaaaaaa ctcgcttttt gccggattag gcccaatttc taatgctaag gtttttacac
     3961 ctttaattcc tgttggatcg taaataaaag gaagcgatgt gttggaacac ccattcgaat
     4021 acgtgcaagt gagcgtatat gtaccaccta tttcacccaa attatagact tgatttgttg
     4081 ctccagaaat cggattgcca tctaaattcc aaacataggt aaccgcatta gcattggttc
     4141 ccaagctgtt gctgacagag aaaaaattaa tattgggaat aggtgctact ccaaatgaat
     4201 aatctaatgg caaactcgtt tcaccataat caggacaagt gccggtaaaa gctgttaccg
     4261 tgtatgttcc attggtagta acggtataag aactttctga ctgaccttcg attaatgaac
     4321 cattcttgaa ccaagaaatc gaattggtaa atggatttcc aacagttaat attacagagt
     4381 ctccctcaca caatgtttct ccatttggga attccgaaat cacataaata ggcaagaaaa
     4441 cccaaccatc taccaaaacc gaaggcgaca tttcggtgca accatttact gtggtttcta
     4501 cgctgtattg agcaccagca gctgaaaatg catcaacaac taaaaaagaa tctgtcgcac
     4561 caattagagc aacgccttcg cgataccact gataagaatc ggcggcttgg gtaaaaagtg
     4621 tatcggttgc tccaggacaa agtatcaaat ctgaaggagt tacaattgga tcttgcgtgc
     4681 attcctgaac aaaagaggta tagttataag gcgtagaata gcgagaacat gtacctgaaa
     4741 aaattgcttc cacagtgaat aacccattcg cttgaggtaa ataggttgaa ctcgttgcat
     4801 ttgttattgg attgctatcc aaataccatt gataggaacc agcaattgta cttgtactca
     4861 aagataaatt cccagaatta aactgaatga ctggcgtttg ggaagctgta aaattgaaaa
     4921 ccgttgccgc acttgtttga ctatacaatg ggcattgatc agtaaatgca ataaccgaat
     4981 atgaacccga ttggtttaca ttaagcgtat cgttgttttg tccgttaagc tgaaccccat
     5041 ttcgaaacca aagaatattc tcgttatacg gatcatttat gataagctga cgcggatctc
     5101 cttcacatcc ggttaacgga agattctctc cgacagaaag agcaattgca ggtggattat
     5161 aaactgacac agcagctgca ggagatgctt cagtacagcc attaatagta gaagcaacga
     5221 tgaaattttg ccctccatca gtcgctgaat taacaaccaa ataaggatgg ttagaatttg
     5281 ctaccggatt tccacctcga taccactgat aagttgttgc tgtctctgtc cacaatgtgt
     5341 cggaactatt cggacaaagt tgcaaagttg aaggcgtaat ggtcggattg aatggacatt
     5401 gtccaaaaat gttgaccaca atacctacgt taaaaagcca aaaaagaagc gttttcatgg
     5461 cgttagtttt tgtgaaaaag gttgaagagg tgaagttact aaataatagc agctttctca
     5521 gctcaaatta aaacaatatt aatcttagcc aattcgctgt ctaaccactt cgtaaacgac
     5581 caaagcagtt gctaccgata cattgagaga acccgttttt ccaaacatgg gaattttcac
     5641 acgttcatcg cagcgtttga gatattccgg actaatccca tcttcctccg agcccatgat
     5701 aattcctaat ggaccttcaa gtttggttgc gtatagcgga ctttcggttt tctcggtaca
     5761 accaaccaaa cgaattcctg agtttttcag gtagtcgagt gtgtctttta aattagttga
     5821 acgacaaaca ggtaaattca tcaatgctcc cgcagaggtt ttcatcgcat cgccacctac
     5881 acgtgcactt ccgcgcgaag gaatgatcac ggcatcaatt ccggcacatt cggccgtccg
     5941 acaaattgct ccaaaatttc tcacatccgt tacccgatcg agaatgagta aaaaaggatc
     6001 tcgaccagct tcaaacacac gttgaagaac ttcttcgata ggttgaaaag taacaggaga
     6061 aataaaacca atcacacctt gatgattagc ccgtgtcata cgatctagct tttcacgcgg
     6121 taccaattga agaggtattt ctaactcatc aagcaattca cgcaattcgt gcaattgagg
     6181 agaccgcaat ccggtttgca tgataacttt atcgacatca gccgaacttc ggagtgcttc
     6241 caacacagga aacatcccaa aaatcatttc cgatgtgtct tgcgcaggtt tgtggtgaaa
     6301 aggtttattc atagctaaat gcgtttccat tcctccagct ttcaacggct ttataccaaa
     6361 cgtttgcata ggtagctttt cgttcaagcc ggtattcgtc ctgataataa gcctgactaa
     6421 ttcgttcgaa ccggaaacta agtggagttg aacccgattt cggattgtaa ttccacactt
     6481 ctgcccgacc gttgttatag acgctagatg gaggtccaaa tatcgtataa atcatcccgc
     6541 ggtcggttct ccaacctggc acgaatgagg taaaaagcaa attagcatgt cttattctat
     6601 ccagccacat cttctcaact gcttctgaat gagccttatc aagctttaga aacttccata
     6661 aatgaaaatc agaaacaccg gaaaattgtt ttatttcttc tttctgcatc aagtaaacca
     6721 tgcatctgaa tcgctccgaa tcattgctca aaccaggaaa attagagcct actacaagta
     6781 aacctagtcc agaagtgggt gaatttccac tagaaattct cagaaactct tgcttctcaa
     6841 atcgaaaatc aagcaattca tctcgcgaaa tagactcttt caaaaatgca tcaggaaaag
     6901 gttttaattc acttgaaaca gcgaaaggag gagcaggcaa agggaattct gccacataat
     6961 gctcaactat aaaccgctgt tctaacttat ccacataccg gatattacac ggatttccaa
     7021 ttcgaaccca agtatgtatg ctgggatttc ctttctcaaa tacctgaaaa tcgagcgcat
     7081 ttccttcctt caaccgactc aaattcacat atttaaatcg agagcgattc ttttgcaaat
     7141 cccgaatatc aagttccaat aatccatctg ttccagtcaa cataggtaaa tgcaattcgg
     7201 ctccggtaaa aatggcggta tctgaaagtc ccgattcgag caccaatggc tgacttacaa
     7261 tgggtttccg gcgtttgaat tcgggataaa atgaagctcg aattaacaat ctacgcttgg
     7321 gttccaaatt atcggcatcg ggcatcaaac ctaaatcggc attcaggaaa cgtaaacgaa
     7381 tggccacact atcggtagat attgcccaat actgcagctc cattggcgga ccaggttgag
     7441 gaaaacgata atcgagatat cgattttggg aaaatggatt tctattcgcc gaacatccag
     7501 ccaacaaaac aaggagaaat aaactaagcc tcgtcttcgg catccggatc ggcacgatta
     7561 agcaatgcag ttggtaatac tttggatgaa cgagctccta atttcttgag tttttctacg
     7621 cgtgtaatca agttaccact gccgtcggta agcttcttca tcgattcttc gtaggtcttt
     7681 ttagctgtat ccatctgatt tcccaactta ataagatcca cactaaaagc tacaaactta
     7741 tcgtacaact tcccgccttg atcggcaatt tcctgggcat tttgattctg atactcttga
     7801 cgccaaatac ttgcaatcgt gcgtaaagta gccagtaaag ttgttgggct aactaccaca
     7861 atattcttat caaac
//
LOCUS       DAOOQW010000103         7860 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_11421_length_7860_cov_4.237284, whole genome shotgun sequence.
ACCESSION   DAOOQW010000103 DAOOQW010000000
VERSION     DAOOQW010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 7860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7860
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11421_length_7860_cov_4.237284"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1126)
                     /locus_tag="PK281_07015"
     CDS             complement(<1..1126)
                     /locus_tag="PK281_07015"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HOY49134.1"
                     /translation="MFVAAVGSDLNIENCKDISGDDDFAVSGSIFTADYTIGGFETLA
                     ANLAAAANSICGTELMINKTVSNSGVCSGETVQFTIQVTNTGGDYNFTAFNVHIEDVF
                     PNGFSNIAFVGSAPAGASISGSTLTYNLASLAANATATVVVSATVDVPPGNFNNIATA
                     IADNANQVSDNASVVSGYATETLTLSECAPVTVNGITYSSTGSYTQELTSAAGCDSIL
                     TINFTLKSATTGVEQAAACASYFWHGTTYTSSGQYTYTTVNAAGCDSVVTLYLNITPA
                     PEQPTLACYQNATFNSTTCSWDVTGDMPAEPTGLACYQDAVFNTTNCSWDIVGTMPAQ
                     PSIACYESASFNGQTCSWDVTGDMPAQPSIACYESASFNGQ"
     gene            complement(1104..>1469)
                     /locus_tag="PK281_07020"
     CDS             complement(1104..>1469)
                     /locus_tag="PK281_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49135.1"
                     /translation="FQNYLYGSSGVRYNPIASGNCIGWTNWEDALVDVQTLDADLVIF
                     FTDGNPTAYNVAKANGCAGGTVTTADDNSYSSNTALNRAITEANDVKAQGKHMFVAAV
                     GSDLNIENCKDINVCCCSW"
     assembly_gap    1470..1569
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1570..1860)
                     /locus_tag="PK281_07025"
     CDS             complement(<1570..1860)
                     /locus_tag="PK281_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49136.1"
                     /translation="MQHNSNLNSSGRFWNLDKLKNTAKAMLLVLFSSFLQTAIAQTET
                     KNTGGQSPTFSTPIKDFRASYSAPDQLIVPKGMTEKQVISMINQSTRQAKTNN"
     gene            complement(2480..2551)
                     /locus_tag="PK281_07030"
     tRNA            complement(2480..2551)
                     /locus_tag="PK281_07030"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2516..2518),aa:Arg,seq:cct)
     gene            complement(2606..3931)
                     /locus_tag="PK281_07035"
     CDS             complement(2606..3931)
                     /locus_tag="PK281_07035"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOY49137.1"
                     /translation="MKTPKIKDAFANLKNKTQISDESNDLLVKKGNQLHIIILVLVAI
                     IAVAYVLFDFIENNYTQAFISATILPSLVFSYWLYHTNRTALSKIWNLTHVTLIIGIQ
                     FLLQPPFSLALTLYLPVLLSTLIVFQGKDRKLGYLLAFLVFCVTIFLIKWGDLFRLNH
                     LVRHETLETLRTINMTGAIGVTILEVLFLLKLNNSVQFELLEIGKNLNRNNEILASTI
                     QTRDKMLSIISHDLRSPLIIIDSGLEVLQEDDFPEDKKKHLSLELRKRSKLTLNLIDN
                     LLLWTRSQTDQIQYKSQPISTNQIGKIIQGFSDTYRGEKGVEMTFDLPETGAVNADKD
                     MLEGILRNLISNALKFTPNGGKISIQIEPIGKRWRFVVQDSGVGIPSEVLEKLKNGDS
                     ISTTGTNKEKGHGLGLQLVRDFLLKHGSELRIESEPEKGTTFSFDLESA"
     gene            complement(4133..7846)
                     /locus_tag="PK281_07040"
     CDS             complement(4133..7846)
                     /locus_tag="PK281_07040"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HOY49138.1"
                     /translation="MKSPFLYIRIFCLLVLWLSPSLYSQNQQLTFERVPSQKWLYQRN
                     ITCLTQDRDGYLWFGTNNGLYRFDGYSVTEYRHEAHNTNSLSHNNVNCIYADKAGVLW
                     VGTWGGLTRIDPNTQSFTRYKHDPRNIKSLSNNDIRAIQEDLAGNLWLGTFGGGINRL
                     DKSSGDFRIFQHSSGNASSISSNYINVITRDRNGDLWIGTRRGINKLEPENSRFTSFQ
                     SLEPTAEDQQWANISSILEDSKGRIWFGTFGGGLIQLDRRSGKMLNYQHDAKNPQSLS
                     NNTVNGLSEEDGGNLWIATSEGINILHLNTGEMQLVQNNPDNPNSLLNNDVHLLYRDR
                     AGVIFIITADGINIYSKLSGRFRKFQKNPNEVKSLSSNAINCFIEGPDGSIWIGTRDG
                     LNRFDRSSKQFQVFRLIPEVKDEVCSNEIKSLHFDQKGRFWVGTSNSLCLFDPSDSKA
                     KQYRFNNFNPNSLNNEVHTIAETRNGALWVGLRKGLAAFDPDSGTFKLYRPDPTVSDN
                     QITNSVYAILEDRYGKFWVGTLGGGLSEFDRKSTKFKSYLNIPNDSTSLSHNSVISLH
                     EDQFGFFWVGTFGGGLNRMDRKTGKFINYTARNGLPDDMIYSILEDPKGNLWMSTNTG
                     LVKFDTKSKAFRNYDALDGLQSNEFSIGASLRCKNGEMLFGGIAGFNLFYPDSILENN
                     YIPPTVITGFKVLNKPVILSGDKVQLTYNQNFFTFEFSSLSYALSDKNQFAYKLDGFD
                     EDWIYCDSRRFASYSNLDPGEYVFHVKSSNSDGLWNEKGTTVTLNIARPFWRTWWFLL
                     IGILLGTSILYFGFRLRTRSINSRNQLLEEKVRQRTAELERATEEAQAAKEAAEKASR
                     SKSGFLANMSHEIRTPLNGILGFTDLLIRNNKNTEDTKYLELIRSSGDTLLNLLSDIL
                     DLNKIEQGKLTIENIRFNFIETIKQTLIPYQYRANEKGLQFMMSFDTRIPEIIQGDPT
                     RIKQLVINLVSNSLKFTETGGVSISFEAESDPRGTEDFFYISGEVTDTGIGVAHEKQN
                     LIFESFTQADGSFTRKYGGSGLGLSIVKQLLHLMKGSIVLSSPALNKPFATETPGATF
                     KFRFRVKAEKTDEKSSKPAVAPPIQTFRFTEKYRILLVEDNKINQLLAVTILENFGLE
                     VKTADDGQQGVEKIKQEDFDLILMDVQMPVMNGYESTAAIRAMGMNLPIVGLTANVYK
                     EDIEKCLESGMNAHLGKPFTEADLFGMLKRWLV"
BASE COUNT         2352 a         1513 c         1436 g         2459 t
ORIGIN      
        1 tttgtccgtt gaatgaagct gattcataac aagcgattga tggttgagct ggcatatcac
       61 ccgttacatc ccaagaacaa gtttgtccgt tgaaagaagc tgattcgtaa caagcgattg
      121 atggttgagc tggcattgta ccaacgatat cccaagaaca attagttgtg ttaaaaacgg
      181 catcttgata acaagctaaa ccagttggct cagcaggcat atcacccgtt acatcccaag
      241 aacaagtagt agagttaaac gttgcatttt ggtaacaagc taaagtcggt tgttctggag
      301 ctggagtgat attcaagtat aaagtaacaa ccgaatcaca tccagcagca tttactgtag
      361 tgtaagtata ttgtccgctt gatgtgtaag ttgtaccatg ccagaaatat gaagcgcaag
      421 cagcggcttg ttcaacacct gttgtagcag acttcaaagt gaaattgata gttaggatac
      481 tatcgcaacc agcagcactt gttaattctt gtgtatagct tccggttgaa gagtaagtaa
      541 ttccattaac tgttactgga gcacattcag aaagtgtaag tgtttcagtt gcgtagccag
      601 atactacact agcattatcg cttacttgat tcgcgttatc tgcgattgca gtagcgatat
      661 tgttgaagtt tcctggagga acatctaccg ttgcagaaac aacaacagtt gcagtagcat
      721 tagcagctaa actagctaag ttgtaagtta gtgtactgcc tgatatagaa gcaccagctg
      781 gagcagatcc tacaaatgca atatttgaga atccgttagg gaatacatct tcaatatgta
      841 cattgaatgc tgtgaagtta tagtcaccac ctgtatttgt tacttgaatg gtgaattgaa
      901 cagtttcacc agaacataca cctgaattag atactgtttt gttaatcatc aattcagttc
      961 cacaaatact atttgcagca gcagctaaat tggcagctaa agtttcaaaa cctccaatgg
     1021 tgtaatctgc agtaaaaata cttcctgaaa cagcgaagtc atcatcacca gaaatgtctt
     1081 tacagttttc aatgtttaag tcgctaccaa ctgcagcaac aaacattaat gtctttacag
     1141 ttttcaatgt ttaagtcgct accaactgca gcaacaaaca tgtgtttgcc ttgcgctttt
     1201 acatcgtttg cttcagtaat tgcacgattt aatgcagtgt ttgaactgta tgagttgtca
     1261 tctgcagtcg taacagttcc accagcacaa ccattagctt tagctacatt gtaagcggta
     1321 ggatttccat cagtaaagaa gattactaag tcagcatcta atgtttgaac gtcaactaag
     1381 gcatcttccc agtttgtcca accgatacaa tttccactag caattggatt gtaacgaaca
     1441 cctgaacttc cataaaggta gttttgaaan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnna ttgttagttt tagcttgacg ggtactttgg ttgatcatcg aaatgacctg
     1621 tttttccgtc atgcctttcg gaacgattag ctggtcaggg gccgaataag aagcgcgaaa
     1681 atctttaatc ggcgtactaa atgtaggact ctgaccgccc gtgtttttgg tttctgtttg
     1741 tgctattgct gtttggagaa aagacgaaaa caagacaagt agcatagcct ttgccgtgtt
     1801 ttttaattta tccaaattcc agaaacgccc agaggagttt aagttagaat tgtgttgcat
     1861 aaaattggtt ttggttagga aattttttgg gttagggcaa tagtattcca ggacacattt
     1921 gtgtcgttct ttacttattt aagtgcctga aatggagtat tttagttcgg ctttcgaaac
     1981 gaagtcggaa aagccaccga agatttagct gtcttcggat tttagtttct gaaagtccat
     2041 agagttaggt taaggttagt aatacaaaag tatacataaa tgatgtaaga tttgttacgt
     2101 tttggtcaaa aaaaattcat tgccagtgcg aataaaacac ctgccaaaaa cgtaagtgtc
     2161 agaatatcaa taattaatga ttttgaattt ccggaaaacg gaagcaaaaa ttttgacaaa
     2221 aagcatgtca ggattatgcc tttcgttaca tttttaagtt ttagtccttg tttttactta
     2281 ttcgaccagt ataaaagctt cacgaaaaac ggatgataga gcctgaatta ataccgtaaa
     2341 taataggtag ataataagtc aaaatggagt attcatttat tgaatgcctt ataagaaaaa
     2401 tgggaaaggg gatatttcaa acttcttaaa ttatagttga acagaataat taataaggta
     2461 attttctaat agatatttgt ggctccggca ggaatcgaac ctgcatctag agtttaggaa
     2521 actcctattc tatccattga actacggtgc cagtatttgc cttattcaaa ctcaataagg
     2581 gcggcaaaga taaccaagga atcgattaag ccgattctaa gtcaaatgag aaagtcgttc
     2641 ctttttctgg ttctgattca attcttaatt cagagccatg ctttaaaagg aaatcacgca
     2701 ctaattgaag ccccaatcca tggcctttct ctttattcgt acctgttgtt gagatagaat
     2761 ctccattctt cagtttttcc aatacttctg agggaattcc aactcccgaa tcttgtacta
     2821 caaatctcca tcttttacct atcggctcaa tttgaatact gatttttcct ccattgggag
     2881 tgaatttcaa tgcgttagaa ataaggttac gtaaaatacc ttcaagcata tccttatcgg
     2941 cattaactgc tcccgtttcg ggtaagtcga atgtcatttc aacacccttt tctcctctat
     3001 aagtatccga aaatccttgt attattttac caatttgatt agttgatatc ggttgtgatt
     3061 tatattgaat ctggtcggtt tgactgcgtg tccacaatag cagattgtcg atcaagttca
     3121 acgtgagttt agatcgtttg cgtagttcca aacttaaatg cttctttttg tcctccggaa
     3181 agtcgtcctc ttgtagaact tctaaaccag aatcaataat tattagcgga cttcttaaat
     3241 catgcgaaat gatagagagc attttgtcac gagtttgaat agtagaagct agaatttcgt
     3301 tgtttcgatt gagatttttg ccgatttcta atagttcaaa ctgaacagaa ttattcaact
     3361 taagtagaaa aagcacttca agaattgtta ctccaatggc accagtcata tttattgtgc
     3421 gaagtgtttc taatgtttcg tgccggacaa ggtgatttaa tctaaataaa tcgccccatt
     3481 ttatcagaaa tattgtaacg cagaagacaa gaaaagccaa taagtatcca agttttctat
     3541 ccttaccttg aaatacaatt aatgtactca gtaaaacggg taaatagagt gttaatgcaa
     3601 gtgaaaaggg aggctgtaat aaaaattgta ttcctattat tagcgttaca tgtgttaagt
     3661 tccaaatttt ggaaagagca gttctgttcg tatgatacaa ccaataagag aaaactaaag
     3721 agggtaaaat ggtagcacta ataaatgctt gagtatagtt gttctcaatg aaatcaaata
     3781 atacataagc aactgctatt attgcaacaa gtactaatat gataatatgt agttgattac
     3841 cctttttaac caataaatcg ttcgactcat ccgatatctg agtcttattc ttcagatttg
     3901 caaatgcatc tttaattttt ggagttttca tggacttagt tccaatatgg attttaaggc
     3961 cgcgaaaagt agactataat tactcttctt ctccttctga tttttatcta acgacaaaac
     4021 caacttattg attcataaaa taacggagaa attcaaaaag cttattaaca agtcgttgat
     4081 ttcacaaacg attctgattt taataaacta gttgtttgaa ttattttgag ctttagacca
     4141 accatcgttt gagcatgccg aataaatcag cttctgtaaa tggttttcct aaatgagcat
     4201 tcattcccga ttccaaacat ttttcaatgt cctctttgta gacattagcg gtaagtccta
     4261 ctatcggtaa gttcattccc atggctcgaa tggcggctgt actctcatag ccattcatta
     4321 ctggcatttg cacatccatc aatatcaaat cgaaatcttc ttgcttaatt ttctcaacac
     4381 cttgttgacc atcatcagcc gttttaacct ctagtccaaa gttctccaga attgttacgg
     4441 caagtagttg attgatttta ttatcctcaa ctaataagat gcggtacttc tccgtaaaac
     4501 gaaatgtctg tattggtggc gcgacagctg gttttgaaga tttttcatcc gttttttctg
     4561 ccttaactcg gaatcgaaat ttaaatgttg caccaggtgt ttcggtggcg aaaggtttgt
     4621 tcaaagccgg actgctcaat acaatcgaac ctttcatcaa atggagcagt tgttttacga
     4681 tagataatcc aagtccactt cctccatatt tacgtgtgaa agaaccatcg gcttgcgtaa
     4741 agctctcgaa gatgaggttt tgtttttcat gcgcaacacc aattccagta tcggttactt
     4801 cgcctgagat ataaaagaaa tcttcagttc cccttgggtc agattcggct tcgaaactga
     4861 tagaaactcc tcccgtttcg gtgaatttta aggagtttga aaccagattt atcactagct
     4921 gtttaatacg agtaggatct ccctgaataa tttcgggaat gcgtgtatca aaactcatca
     4981 taaactgtaa tcccttctcg ttcgcacggt attgataagg aattaaggtt tgtttgatcg
     5041 tttcgataaa attaaagcgg atgttttcaa tggtgagctt accttgttca atcttattta
     5101 aatccaagat atcgctcaac aaattcaata aggtatcacc cgacgaacga atcaactcta
     5161 aatacttggt atcctctgtg ttcttgttat ttcgaattaa taaatcggtg aatcctaata
     5221 taccattgag tggtgtacgg atttcgtgac tcatattcgc caagaatccc gatttggaac
     5281 gacttgcttt ctcagctgct tctttagctg cttgtgcttc ttcagtagcg cgttcgagct
     5341 cggctgtacg ctgacgaact ttttcttcca ataactgatt acgactattg atactgcgag
     5401 ttcgtaaacg gaatccgaag taaagaatac tagttccgag tagaatgcca atcaacaaaa
     5461 accaccaagt tctccaaaat gggcgagcaa tatttaaggt aacagtcgtt cctttttcat
     5521 tccacaatcc atcactatta gagcttttta cgtggaaaac atattcgcct ggatcaaggt
     5581 tcgaataaga tgcaaatcga cgagaatcac agtaaatcca atcttcatca aatccatcaa
     5641 gcttatacgc aaattgattt ttatcgctta acgcataact caaagaagag aattcaaacg
     5701 tgaagaaatt ctggttatac gtcaattgaa ccttatcacc cgaaaggata acgggtttgt
     5761 tcaatacttt aaacccagta atgacagtag gtggaatgta attattttca agaatgctat
     5821 cgggatagaa caggttaaat cctgcaatac cgccaaataa catttctcca tttttgcatc
     5881 ttaatgacgc accaatacta aactcattac tttgtaaacc atccaatgca tcgtaattcc
     5941 ggaatgcttt cgatttcgtg tcaaatttca ccaaaccagt attggtactc atccacaaat
     6001 ttcctttagg atcttccagg atgctataaa tcatatcatc tggcaacccg tttcgagcag
     6061 tgtaattgat aaacttgccc gtttttcggt ccattctatt caatccaccg ccaaaagtcc
     6121 caacccaaaa gaatccaaac tggtcttcat gcaaggatat gactgagttg tgtgataagc
     6181 tggtcgagtc atttggtata ttcagatagg atttaaattt tgtcgattta cggtcgaatt
     6241 cacttaatcc accacctaaa gtacctaccc aaaatttacc atagcggtct tctaaaatag
     6301 cgtagacact attggtaatt tgattgtccg aaacagtagg gtcaggtcga taaagtttaa
     6361 atgtgcctga atcgggatca aatgctgcta atccttttct taatccaacc cataatgctc
     6421 cattccgagt ttcagcaatg gtatgaactt cattatttaa actgttgggg ttgaaattgt
     6481 taaagcggta ttgcttggct ttagaatcag acggatcgaa taaacaaaga ctattagaag
     6541 tccccaccca gaatcgacct ttttgatcaa aatgcaagga tttaatttca ttactacaca
     6601 cctcatcttt cacctcagga atcaatcgaa acacctgaaa ttgcttgcta gaacgatcaa
     6661 aacgattcaa cccatcacgc gttcctatcc aaatagaacc gtctggtcct tcaatgaaac
     6721 agttaatggc attactcgat aagcttttaa cttcattcgg attcttttgg aatttcctga
     6781 aacgtccgct gagcttgctg tatatgttaa ttccatcggc agttataatg aaaataacac
     6841 ctgcacgatc gcgatatagc aagtgaacat cattattcaa caaactattc ggattgtcgg
     6901 gattgttttg caccaactgc atctcgcccg tattgagatg aagtatgtta ataccttcgg
     6961 atgttgcaat ccataaattg cctccatctt cctccgaaag tccgtttacc gtattgttgc
     7021 taagcgactg aggattttta gcatcgtgct gataattcaa cattttacca ctacgacgat
     7081 caagttgaat caatcctcca ccaaatgtcc caaaccagat tcttcctttg ctatcctcga
     7141 gaatactcga aatatttgcc cattgttgat cttcggcagt cggttctagg ctttgaaacg
     7201 aagtgaatcg ggaattttca ggttctagtt tattgatacc acgtcgtgtt cctatccata
     7261 aatcaccatt tcgatctcgc gtaatcacgt tgatgtaatt gctcgaaata ctgctagcat
     7321 tgcctgatga atgttggaaa attcgaaagt caccactaga tttatccaag cgatttatac
     7381 caccaccaaa tgtacccaac cacagattcc cagctagatc ttcttgtata gctcggatgt
     7441 cgttgtttga taaggatttg atgtttctag gatcatgttt gtagcgcgta aacgattggg
     7501 tattcgggtc gatgcgagtt agcccgcccc atgttccaac ccataaaacg ccagctttat
     7561 cggcataaat gcagttaacg ttattgtggg ataaactatt ggtattgtgt gcttcgtgac
     7621 ggtattccgt gactgaatat ccatcgaaac gataaagacc attgttggtt ccaaaccata
     7681 aataaccatc tctatcctgc gttaagcaag taatgttacg ttggtatagc catttctggc
     7741 tgggcactct ttcgaatgta agttgttgat tctgtgaata aaggctaggg ctgagccaaa
     7801 gcactaacag acaaaagata cgtatgtata gaaatggact tttcatgaat cagcgattgc
//
LOCUS       DAOOQW010000104         7843 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_11444_length_7843_cov_6.436184, whole genome shotgun sequence.
ACCESSION   DAOOQW010000104 DAOOQW010000000
VERSION     DAOOQW010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 7843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7843
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11444_length_7843_cov_6.436184"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1709
                     /locus_tag="PK281_07045"
     CDS             <1..1709
                     /locus_tag="PK281_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HOY49139.1"
                     /translation="AHSIAAQGGINAAKNYANDGDSVHRLFYDTVKGGDYRSREANVH
                     RLAEVSTAIIDQCVAQGVPFAREYGGLLDNRSFGGVQVSRTFYARGQTGQQLLLGAYS
                     ALSRQIGRKQVDMYNRHEMMDVVLIDGKARGIIARDMVTGKLERFFGHAVVLATGGYG
                     NVFFLSTNAMGSNVTAAWKAHKKGAFFGNPCFTQIHPTCIPVSGDHQSKLTLMSESLR
                     NDGRIWVPKQKDDKRKGADIPEEERDYYLERRYPAFGNLVPRDIASRAAKERCDAGYG
                     VGTSKMAVYLDFSDAIKRLGQPAIEARYGNLFEMYEKITGENPYQTPMRIYPAVHYTM
                     GGLWVDYNLMTTVEGLYATGECNFSDHGANRLGASALMQGLADGYFVLPITIGDYLSK
                     DIRTKAIPTDHPEFAKAEKEIADKIQKLMSVQGKEPVDYFHKKLGLVMWEYCGMARNA
                     EGLQKAKKMVQDIRAEFWSNVRIPGTANETNQELEKALRVADFLELGELMIDDAFNRN
                     ESCGGHFREEYQTEDGEARRDDDNYAYVAAWEFKGEGQQSELHKEELVFDNVKLVQRS
                     YK"
     gene            1737..2480
                     /locus_tag="PK281_07050"
     CDS             1737..2480
                     /locus_tag="PK281_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HOY49140.1"
                     /translation="MKLTLKVWRQKNNQAKGEFKTYPMDHVSEDMSFLEMFDVLNEDL
                     VRKGDEPVAFDHDCREGICGMCSMHINGRAHGPAEGTTTCQLHMRKFKDGETITVEPF
                     RAKAFPVIKDLMVDRSAFDRIIQAGGFVSVNTGNAVDANSLPIPKPDADKAFDAAACI
                     GCGACVATCKNASAMLFVSAKVSQLALLPQGQPERHIRVKRMVEVMDAEGFGNCTNTG
                     ACEVECPKGISLENIARMNRDFLVANAID"
     gene            2829..4127
                     /locus_tag="PK281_07055"
     CDS             2829..4127
                     /locus_tag="PK281_07055"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY49141.1"
                     /translation="MSSIRYYLFLLVFLPFTPVVNAQNLIPNPSFDLVDTCNPSSDWI
                     WNLSSWENLGYYDSIAYGTPDVYNACNIPGFPPIWSFPYTWLTFQYPHSGNGFVGIFN
                     GAFFNRLYLFQEEIDHGREYFAAELNEPMQPQVLYCVGGYMNNRNLNYIPPELTKFWT
                     IRTDRISIGFHNSMPVMNNTWLLSGINHIATLTQEDSSVVYDTLGWAHMRTPYLAQGN
                     ESHIVMGNLLSDTASGFTLHTNMNQDSIYALPMSEVPTSYYRSGAYFYFDDMYVIPIR
                     KPNIEVVDGGNGFVWLVDTTAQEEKTWFKTGDDLLLGTGDSLYLHVSDGASYSLHTRN
                     CRVEMSDTVVIDLTGIEKVNTGAEMRLFPNPSEGILTIDFQGESANVQVYSITGQLLF
                     ERYIISGETISMEPLPTGTYIVELIMKSGFSQHKIVEIIR"
     gene            4204..4761
                     /locus_tag="PK281_07060"
     CDS             4204..4761
                     /locus_tag="PK281_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49142.1"
                     /translation="MRVILIYILIAIQLGYSQRNIVNDEVSDIRTICIEKFVEDYNIL
                     DKESLNVVDFQLDDSVDISPWKNHMFDIKWLDTIIAFDRSDYLDMINENDIITFRDNE
                     FQIPNKPSYLKYNVIQFHRENFYTSDTIGFLRIMPIIFDTRNETSILFLRFYRGIEES
                     VTYAYFLKLINLKWSIQGCKILAIS"
     gene            4808..6391
                     /locus_tag="PK281_07065"
     CDS             4808..6391
                     /locus_tag="PK281_07065"
                     /inference="COORDINATES: protein motif:HMM:NF013252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49143.1"
                     /translation="MYTFSRLCLNLNQAAANQKERILSDYVKSVDEESLAHAFHLLLE
                     NRPERCITSATLRQWVLEESGIPEWLLEESIDMIGDPAAALAMVISDSTIPDPISLPQ
                     VMQVVQNLKTETPENQKIIIAELWKRMDAEQRLIFNKLLTGTFRYKIRPVEAAKAIEP
                     ITTKPISELIQAFSQKWEIHEWSNMVEQSSDINFAPSYVPFRPVSEIISDPEQLGNPS
                     DWLALFTFQGIHVQARVLKKGIQLWTRNEELLPDVFPEVRNAASYFEEGTLLIAELVV
                     QPADDETPGFFSNLIKRIQSKKMRHDLMERSPVKLVVQDMLFEGNKDIRNLSLLERRK
                     RLQARMKKVKDYGFQYAPAIEFKSWDDLRRCQTQIREKLADGISLQKLHAPWDAQALL
                     KLKAAPFEINAVLLYVSKIPDKNKKIITECGFGVWRENASGQPEIISITRSNEGLSPE
                     LIQQLDAWVKLHTRERFGPVRSVDAELVFQLQFDEIQTSRRHKSGLVLQNVRMKMPLE
                     PLSIQQAGTWEQLNAMVSN"
     gene            complement(6577..>7843)
                     /locus_tag="PK281_07070"
     CDS             complement(6577..>7843)
                     /locus_tag="PK281_07070"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY49144.1"
                     /translation="PSFTQVPPVCTGSIMLPLPTLSNNLISGTWLPAMNNTATTTYTF
                     TPDANQCGTTTTMTIVVNPITMPSFTQVSGICAGETLLPLPTTSNNGITGTWSPALNN
                     TFTTTYTFTPNGGQCASEGTMTIFVNPNTTPSFTQVPAICAGVSLLPLPTTSNNGISG
                     SWAPALNNATTTTYTFTPAVGQCASTASMTITVNPNITPSFSPVGSYCAGQSISPLPT
                     TSLNGISGSWSPVLNNTITTNYNFTPSGGQCATTTNTIIVINENPTVTLTFDGAILTA
                     SAGFTGYVWTLNGNTLADATTNEIVVAQVGLYTVTVTDANGCNASATFEVQTVGLANP
                     SFGNEFSLHPNPSLGITTLTLLCTETQTAHIRVLDLQGKIQFQQTNSFNVGKNEVPLN
                     LSNLADGVYFLQLENSNFKHTKRFVKMRE"
BASE COUNT         2340 a         1578 c         1722 g         2203 t
ORIGIN      
        1 gcgctcactc cattgctgcc caaggtggta tcaatgccgc gaaaaattac gcaaacgatg
       61 gtgactctgt gcaccgtctt ttctacgata cagtaaaagg tggcgactac cgttctcgcg
      121 aagcaaatgt gcatcgcctg gcggaagtga gtactgcaat catcgaccaa tgcgtggctc
      181 aaggtgttcc ttttgcacgt gaatacggcg gattgcttga taaccgctca tttggtggtg
      241 ttcaggtttc tagaacattc tacgcacgcg gtcaaacggg acaacaatta ttgctcggtg
      301 catactctgc gctaagtcgc caaattggtc gcaagcaagt cgatatgtac aaccgtcacg
      361 agatgatgga tgtggtattg atcgatggaa aagcccgcgg tatcattgct cgcgatatgg
      421 ttacaggtaa actagagcgt ttcttcggac atgcggtggt acttgctact ggtggatatg
      481 gaaacgtatt cttcctttcg accaacgcga tgggttcaaa tgtaactgct gcttggaaag
      541 cacataaaaa aggagctttt ttcggaaatc cttgcttcac acaaattcac ccaacctgta
      601 ttcccgtttc aggagatcat cagtcgaaat tgactttgat gtctgaatca cttcgtaacg
      661 atggacgtat ttgggtgcca aaacaaaaag acgataaacg taaaggagcc gatattcctg
      721 aagaagaacg cgattactac ctagaacgtc gttatcctgc tttcggaaac ttagtaccgc
      781 gtgacattgc ttcacgtgct gcaaaagaac gttgcgatgc gggttacgga gtaggaactt
      841 ctaaaatggc cgtttacctc gatttcagtg atgcgatcaa acgcttaggt caaccagcta
      901 ttgaagcgcg ttacggaaac ttgttcgaga tgtacgaaaa gattactggc gaaaatccgt
      961 atcaaacacc gatgcgtatt tatccagcgg ttcactatac catgggcgga ctttgggttg
     1021 attacaactt gatgacgaca gtcgaaggct tgtacgcaac tggagaatgt aatttctctg
     1081 accacggagc gaaccgttta ggtgcttcag ccttgatgca aggtttggcg gatggatact
     1141 tcgtattacc gattacgatt ggtgattacc taagcaaaga cattcgtacg aaagcgattc
     1201 caaccgatca tcctgaattt gcaaaagcag agaaggagat tgccgataag attcagaagt
     1261 tgatgagtgt tcaaggcaag gagccagttg attatttcca caagaaatta ggattggtga
     1321 tgtgggaata ctgcggaatg gcgcgtaatg ccgaaggatt gcagaaagcc aagaaaatgg
     1381 tgcaggatat ccgtgctgaa ttctggagca atgtgcgtat cccaggaaca gcaaacgaaa
     1441 ccaaccaaga attagaaaaa gcacttcgcg tagcggattt ccttgaatta ggagagttga
     1501 tgatcgatga tgcgttcaac cgcaacgaaa gctgtggtgg acacttccgc gaagaatatc
     1561 aaacagaaga cggtgaagca cgccgcgatg atgacaacta tgcttacgta gcagcatggg
     1621 aattcaaagg cgaagggcag caatcggaat tacacaaaga agaattggta ttcgacaatg
     1681 tgaaactcgt tcaacgttct tataaataat ccttacagac ctaaaacaac aagcatatga
     1741 aactcacgct gaaagtctgg cgccaaaaaa acaaccaggc caaaggagaa ttcaaaacat
     1801 atcctatgga tcatgtgtcg gaagacatgt cgttcctcga aatgtttgat gtattgaacg
     1861 aagacctggt tcgcaaaggc gatgaaccgg tagcatttga ccacgattgc cgcgaaggta
     1921 tttgtggaat gtgctccatg catattaacg gtcgcgcaca cggaccagcc gaaggaacaa
     1981 ctacctgcca gctgcacatg cgtaaattca aagacggcga aaccattacg gttgaaccat
     2041 tccgtgcaaa agccttccct gtgattaaag atttgatggt agatcgttct gccttcgatc
     2101 gcatcattca agcaggtgga tttgtatccg taaatactgg aaatgcagtt gatgcgaaca
     2161 gcctacccat tccaaaacca gatgccgata aagcattcga tgccgctgct tgtattggct
     2221 gtggtgcttg tgtagcaacc tgcaaaaatg cttctgcgat gcttttcgta tctgcaaaag
     2281 tgagccagct agccttatta ccacaaggac aaccagaacg ccacattcgt gtaaaacgca
     2341 tggttgaggt aatggatgcc gaaggatttg gtaactgtac caacacaggt gcttgcgaag
     2401 tagaatgtcc aaaaggcatt agcctcgaaa acatcgcccg catgaaccgc gatttcttag
     2461 tagccaacgc gattgactaa tactatgaat ttataaaatt gaaaagaggg cttcggtcct
     2521 ctttttttat gtttaaacgt tttctattca ttcggtttga aagaataaat tgaaatatag
     2581 tagaactgaa atctagtaat cgaaaaaatc cagcaataaa gctcccctct cctgccacat
     2641 agtggtccct atcccatagg gatcactatg tgacgggacg gagagggggc tagggggtga
     2701 ggtcaacgaa tgccccaatt taaattgcat ttatctgaaa agggaacgcc tccaaaccaa
     2761 ccgacacctt aaattatctg ttctcttcta tcgcgacagc tttggtatat ttacgtattc
     2821 gattctttat gtccagcata cgctattatt tatttctgct agtattcctg ccttttactc
     2881 cagttgtaaa cgcccaaaac ctcattccca atccgagttt tgacttggta gatacgtgta
     2941 atccttcttc agattggatt tggaatctat cttcttggga aaaccttggg tactatgata
     3001 gtattgcata cggaacacct gatgtttata atgcatgcaa tattccaggc tttccaccaa
     3061 tttggagttt tccatataca tggttaacct ttcaatatcc gcatagtgga aatgggtttg
     3121 ttggaatttt taatggtgct tttttcaatc gtctttattt atttcaagaa gaaatagatc
     3181 atggaagaga atatttcgca gcggaactca atgaacctat gcaaccacag gtattgtatt
     3241 gtgtaggagg atatatgaat aaccgtaatc ttaattatat acctcctgaa ctaacaaagt
     3301 tttggactat tagaacagat cgtatttcaa taggtttcca taattcaatg ccagtgatga
     3361 ataatacttg gttgctttcg ggtattaatc atatcgcaac actcactcaa gaagattcat
     3421 ctgttgtata cgatacattg ggatgggcac atatgcgtac tccttacctc gcacaaggaa
     3481 acgaatctca tattgtgatg gggaatttat tatcggatac tgcttctggt tttacacttc
     3541 atacaaatat gaatcaggat tcgatttatg ctttgcccat gtccgaagtt cctacctctt
     3601 attatagaag cggtgcttac ttctattttg atgacatgta tgtgatcccc atccgcaagc
     3661 cgaacattga agttgttgac gggggaaatg gatttgtttg gttggtggac acaactgctc
     3721 aagaagaaaa aacctggttc aaaaccggcg atgacctttt gctcggaacc ggcgatagtc
     3781 tgtatctcca cgttagcgat ggcgcgtcct attcgctgca cacccgaaat tgtcgcgttg
     3841 agatgagcga tacggttgtg attgatttaa cggggattga aaaagtaaac acaggagcag
     3901 aaatgcgttt gtttcctaat ccatcggaag gcattctcac tattgatttt caaggagaaa
     3961 gcgccaacgt gcaggtgtat tctattaccg gacaattact atttgagcgg tacataattt
     4021 ctggcgaaac gatttcaatg gagccgcttc caacaggaac ctatatcgtt gaattaatta
     4081 tgaaatcggg attttcccaa cataaaatag tggagattat tcggtgagat gaaattaggg
     4141 ggtaatttat tacgtctaca actataaaaa ctgtactatt caaattcagc ttaattattc
     4201 gaaatgagag taatattaat ctatatatta attgcaattc aactcggata ttctcaaaga
     4261 aacattgtta atgatgaggt ttcagatatt agaacaattt gcattgaaaa atttgttgaa
     4321 gattataata ttttagataa agaatcatta aatgttgttg atttccaatt ggatgattct
     4381 gttgatattt caccatggaa aaatcacatg tttgatatta aatggctcga cacaataata
     4441 gcatttgatc gttctgacta ccttgatatg attaatgaaa atgatataat tacgtttcgc
     4501 gataatgaat ttcaaattcc gaataaacca tcttatttaa aatataatgt tattcaattt
     4561 catcgtgaga atttctatac atctgataca ataggttttc taagaataat gccaattata
     4621 ttcgatacta gaaacgagac ttcaatttta tttcttcgat tttatagagg aatagaagaa
     4681 tcagttacat atgcttattt tctgaaattg ataaatttga aatggtctat tcaaggatgt
     4741 aaaattcttg cgataagcta aactgcctct aactattcga taatcattaa gattctattt
     4801 aaaaaaaatg tacaccttct cccgcctatg cctcaacctc aatcaagctg ctgctaacca
     4861 aaaagagcgg attctatccg attatgtaaa atcagtcgat gaagaaagtt tagctcatgc
     4921 ttttcacctg ctgcttgaga atcgtcccga acgatgcatc acttcggcaa cgcttcgtca
     4981 atgggtgctc gaagaaagtg ggattcctga atggctatta gaagaaagta tcgatatgat
     5041 tggtgatcct gcagctgctc tggcgatggt catttcagat tcaaccattc ccgatcctat
     5101 ttcactgcca caagtaatgc aggtagttca aaacctgaaa accgaaactc cagaaaatca
     5161 aaaaataatc atcgcagaac tctggaagcg catggatgct gagcaaagac tcatcttcaa
     5221 caaattgctc acaggcactt ttcgatataa aattcgtcct gtcgaagcgg caaaagcaat
     5281 cgaacctatc accaccaaac ccatttcaga actcatccaa gccttctccc aaaaatggga
     5341 aatacacgaa tggtcaaaca tggtggaaca atccagcgat atcaattttg ctccatccta
     5401 tgtcccattt cgtccggttt cagaaatcat ctcagatccg gaacaattag gaaacccgag
     5461 cgattggcta gcacttttta cctttcaggg aattcatgtt caagcccgcg tgctgaaaaa
     5521 aggcattcaa ctttggacgc gcaacgaaga gcttttaccc gatgtttttc ccgaagtacg
     5581 caatgctgcc agctattttg aagaaggtac gttattgatt gccgaattag ttgttcaacc
     5641 tgccgatgat gaaactccgg gatttttttc caacctcatt aaacgcattc aaagcaagaa
     5701 aatgcgtcac gatttgatgg aacgatcgcc tgttaaattg gtcgttcagg atatgttgtt
     5761 tgagggcaac aaagacatcc gcaatttgtc cttactcgaa cgaaggaaac gtttgcaagc
     5821 gcgaatgaag aaagtgaagg attatggatt tcaatatgct ccagctattg aattcaaatc
     5881 ctgggatgat ttacgacgat gtcaaaccca aattcgtgaa aagctagccg acggaatttc
     5941 cttgcaaaaa ctccatgctc catgggatgc tcaagcgctt cttaaattga aagctgcacc
     6001 attcgaaatc aatgcagtac ttctttatgt gagcaaaatt cccgacaaga ataaaaaaat
     6061 catcaccgaa tgtggctttg gtgtttggcg tgaaaatgca tccggacaac ccgaaatcat
     6121 ttccatcacc cgttccaacg aaggactttc tcccgaacta attcaacagc tcgatgcttg
     6181 ggtgaagctt catacgcgcg aacgatttgg tccagttaga agcgtagatg ccgaactcgt
     6241 atttcaactt caatttgatg aaatccaaac aagccgccgc cataaatcgg gcttagtgct
     6301 acaaaacgtt cgcatgaaaa tgccgctcga acctttatcc attcagcaag ctggaacttg
     6361 ggagcaattg aatgctatgg tatccaatta aatactaatt gagttcctaa tttttaaagt
     6421 aaaattggtt cctgtttttc aaacgaatag agaattttag attccgatta aggcttttaa
     6481 aacatagaaa aaaccttaca aaatcgatat aggaatatcc aactactgaa taggctttac
     6541 aacctatcaa ccttccaaac attccaacta taaatcctat tccctcatct taacaaaccg
     6601 tttggtgtgt ttgaaattcg aattttctaa ttgaagaaaa tacactccat ctgccaaatt
     6661 actgaggtta agtggcactt cgtttttacc gacattgaac gaattggttt gctggaattg
     6721 tatttttcct tgaagatcta aaacacggat atgtgctgtt tgcgtttcgg tgcaaagtag
     6781 ggttaacgtt gtgatgccaa ggctagggtt cggatgcaaa ctaaattcat taccgaaaga
     6841 agggtttgca agtcctaccg tttgaacttc aaatgtagcg gaggcgttac atccattggc
     6901 atcagtcacc gtaaccgtgt aaagcccaac ttgagcaact actatctcat tggtagtggc
     6961 atcagctagt gtgtttccat ttaaggtcca tacataacca gtaaagccag cacttgcagt
     7021 gagtatcgcc ccatcgaagg tgagcgttac tgttgggttt tcatttataa cgatgattgt
     7081 gttagtagta gttgcgcatt gtccacctga tggggtgaag ttatagttgg tggttattgt
     7141 attgttcaac acaggagacc agctacccga aataccattg agcgatgtag taggcaatgg
     7201 cgaaatcgat tgtcccgcgc aatacgatcc aacaggcgag aagctaggcg tgatgttcgg
     7261 gtttactgta atggtcatgg atgcagtgga agcacattgt cccacagcag gggtaaaagt
     7321 ataggtagtt gttgtggcgt tattcaatgc tggtgcccaa gagcctgata tgccgttgtt
     7381 cgatgtggtc ggtaaaggca acaaggaaac tcctgcgcaa atggccggaa cctgggtgaa
     7441 gcttggcgtg gtgttcgggt ttacgaaaat ggtcatcgtt ccttcggatg cacattgtcc
     7501 tccgttaggt gtaaacgtat atgtcgtggt gaaggtattg tttaaggctg gtgaccaagt
     7561 tccagtgatg ccgttgttcg atgtggtggg taaaggcaat aaggtttctc cggcgcaaat
     7621 accagatacc tgagtgaagc taggcatagt gatcgggttt acgacaatgg tcatagtagt
     7681 ggtggttcca cactgattgg catcgggcgt aaatgtgtag gtggtggtag cggtgttgtt
     7741 catggcaggc aaccaagtac ccgaaatgag gttgttcgat aaggtaggca aaggcaacat
     7801 gatgcttccc gtgcaaacag gtggcacttg ggtaaagctc ggc
//
LOCUS       DAOOQW010000105         7784 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_11551_length_7784_cov_6.996765, whole genome shotgun sequence.
ACCESSION   DAOOQW010000105 DAOOQW010000000
VERSION     DAOOQW010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 7784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7784
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11551_length_7784_cov_6.996765"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..588)
                     /locus_tag="PK281_07075"
     CDS             complement(<1..588)
                     /locus_tag="PK281_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49145.1"
                     /translation="MKNTYFCSTGLLSVLDQRFIQLKNMTPKFLVISFALLLILVGGQ
                     IGSAQTVIQIGTGTLSPSVGTNPAAGIEGCSPYGVNVSSGAGGKKVQLIYTKAMIDAA
                     MTTAGFTPGTAFIHNVGFNITGIVTSGFNHQNYTIKMANVAQADLSGGYYTGASTTVY
                     GPTSFAPTATGWYSLAIGTPFQWDGTSNLLVEVCYT"
     gene            complement(1110..1601)
                     /locus_tag="PK281_07080"
     CDS             complement(1110..1601)
                     /locus_tag="PK281_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATPase"
                     /protein_id="HOY49146.1"
                     /translation="MEKTFESIRDTDRIWVYQANRILSEEESTYIKKSGMLFVSGWAA
                     HGKPLRADVLVIRNLFVVVMVDEAQAKASGCSIDKSVSWINAIGQELRIDFLDRMQIA
                     WLDDNGSLQINPMAEFETLASNGQIESDTLVFNNLVFSGKELKNSWLVPANESWHNRF
                     LVK"
     gene            complement(1606..2391)
                     /locus_tag="PK281_07085"
     CDS             complement(1606..2391)
                     /locus_tag="PK281_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOY49147.1"
                     /translation="MELNVKTMAQYLANGEVPEILFWVGCAGSFDDRAKKVTKAVAKL
                     LQAADVKFAVLGSEESCTGDPAKRAGNEFLFQMQAMQNIAVLNGYEVKKIVTACPHCF
                     NTLKNEYPELGGHYDVVHHSQLIESLLKDGRLKVEGGSFKGKKITFHDPCYLGRANGE
                     YEAPRAVLGKLDAELVEMKRCKTKGLCCGAGGAQMFKEAEPGDKEVNVERSEEALALN
                     PTIIAVGCPFCNTMMTDGVKHAEKEASVSVLDIAELIANAADL"
     gene            complement(2465..2821)
                     /locus_tag="PK281_07090"
     CDS             complement(2465..2821)
                     /locus_tag="PK281_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006266950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HOY49148.1"
                     /translation="MKENLIQQKTYDFALKIINTSRQMNKNKEFVLSRQLLKSGTSIG
                     ANVEEAIGGQSRKDFLSKISIAYKEARETKFWIRLLRDSNTIEPTHSEDLLIQIDEIL
                     RIIGSIQRTTKGKLHN"
     gene            complement(2922..4229)
                     /locus_tag="PK281_07095"
     CDS             complement(2922..4229)
                     /locus_tag="PK281_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009779418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOY49149.1"
                     /translation="MGLQNVLFILFFLAGAALFTVNVRKIIRNIRLGRKLDRTDRKAE
                     RWNIMFRVALGQSKMVVRPTAAILHLLVYVGFVIINLEVLEIILDGILGTHRLFAPFL
                     GGFYDFLIGSFEFLALGVLVAVIIFFIRRNIIYIKRFRQKELNGWPRSDANTILMIEV
                     LLMTAFLTMNAADQVLQTRGVEHYIQAGSFPVSSLITGLFSSWSDGALIAYEHGAWWF
                     HILGILAFLNYLPYSKHFHIILAFPNVWYSNLEKKGRFTNMAAVTNEVKLMLDPSAVP
                     VELDGPVKFGAKDVQDLTWKSLLDAYTCTECGRCTSECPANQTGKLLSPRAIMMKTRD
                     RLEEVGKNIDANQGEFKEDDKSLLHDYISPEELWACTTCNACTEACPVNIDPLSIIID
                     LRRQLVMEESAPPAALSAMFTNVENNGAPWQFSPSDRLNWKDE"
     gene            complement(4283..5257)
                     /locus_tag="PK281_07100"
     CDS             complement(4283..5257)
                     /locus_tag="PK281_07100"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HOY49150.1"
                     /translation="MQISREIRVGVVVTLGIVLLYFGFNFLKGKNLFSPSRSYYAVFD
                     NVDQLMPSARVQVNGFQVGIVDQVGFLSEGSYKVLVKFLIDNDDLNIPKDSEAHIVQD
                     LLGTRTLELTLGKSTQLAERGDTLMAVRDAGITDEIKKAILPLKAQIEALAGSVDSVL
                     TGFNNVFNRKTQDGLISSFESVNTSIKRFEHTVNEFDLLVTNERHKLSSILSNVASIT
                     DNLKNNNEKLSHVFTNMDRISDDVAKSNVKQTLTDLQETIASLNKVLKNVESGQGTLG
                     KLATDDSLYVNLDHSSRNLSLLLEDMKAHPNRYVHFSVFGKKEKAPKK"
     gene            complement(5274..6482)
                     /locus_tag="PK281_07105"
     CDS             complement(5274..6482)
                     /locus_tag="PK281_07105"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOY49151.1"
                     /translation="MKTFLKYRFTFLAILLISGVTLAFLPKLEADKNLHRESGIRTIV
                     IDPGHGGHDSGCHGAAAYEKTVALAVSLKLGKLIEANFPDVKVIFTRKTDVFVELYRR
                     AQIANENKADLFICIHCNSGPKTAYGAETFVMGLHKSTDNLNVAKRENAVILQEDNYQ
                     KKYDGFDPNSAEANIIFSLYQNAFLDQSLYFADRIQHEFKTYATRHNRGVKQAGFLVL
                     YRTTMPSVLIETGFLTNGEEEKYLKSGTGQQKIANSILRALNSYKSWIEGTGANNDLK
                     FPDEEQEHVQEVKPSEHHVKPDPKVEKTEEAKNQTAEDNSGIIYRIQVYTSPEKVSLK
                     SARFKGRTDVWEYKFNKSWKYTVGRCEKQEEALKLLSELKKDGFSDAFVVAFRNNQRI
                     TLQEAQAIQP"
     gene            6637..>7784
                     /locus_tag="PK281_07110"
     CDS             6637..>7784
                     /locus_tag="PK281_07110"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HOY49152.1"
                     /translation="MSSRAQTPDRNVVDADSLLALQLKEDTVRKSGSLPPGSLPPSDD
                     ALDSRVEYKARDSMRFDLKLKKIFLYGAADVKYDKIHLTADYIEINWGNNVVHAEGRP
                     DSTGKIIGHPVFDEGTQTFDADRMDYNFKTKKGRISGIFTQQGDGYIHGEQVKKSEKD
                     EFYIRNGKYTTCNLPDHPHFYINASKIKVMPGDKIVSGPANLWIEDVPTPLAVPFGIF
                     PNSTKRKSGIIFPTYGESPGLGFYLQEGGYYFGINDYIDASIRGNIYSRGSWGLSAGT
                     SYNYRYHFNGSLDVRFSKILQGDPELLGSSINKDFFITWKHTQDPKSRPNSRFSASVQ
                     AGSSNYNKLNSLNANNIVANTFTSSISYAKTFANTPFNMVISAGHSQNT"
BASE COUNT         2280 a         1708 c         1374 g         2422 t
ORIGIN      
        1 tgtatagcaa acttcaacca ataaattact tgttccatcc cattgaaaag gtgttccaat
       61 agctaaactg taccatccag tagctgtagg tgcaaaactt gtcggtccat aaactgttgt
      121 tgaagcacct gtatagtatc ctccagagag atcagcttgt gctacattgg ccatcttaat
      181 ggtgtaattc tgatgattaa aacctgatgt aactatacct gttatgttaa atccaacatt
      241 atgtataaac gcagtgccag gagtaaatcc cgctgttgtc atagctgcat ctatcatggc
      301 tttggtatag atcagttgca cttttttacc accagcacca gaactaacat tcacaccata
      361 tggagaacat ccttcaattc cagcagccgg atttgtacct acactcggag ataaagtacc
      421 tgttccgatt tgaataactg tttgggccga gccaatttga ccaccaacga gtatcagtag
      481 tagagcaaaa gagataacca gaaatttagg tgtcatgttc ttcaactgta taaatcgttg
      541 atctaatact gataatagac cagttgagca gaagtaggtg tttttcataa gcacggtaac
      601 atatgttatg tgaggttctt tttaagaatg gctgagcgga aaattgccat catttcaaac
      661 ctaccttaaa taataccacc gtaaaattct ggtagtcgag cctatctaat ttgttgattc
      721 tgtttatgtc aactttaact gtcggaaaat taacagttca ataagatgta atagaaaggg
      781 gtaacttgat gaaaccaaga actgctttta atgcaatgcg ctgaaaatca agctaaaata
      841 gccagttgga ttgtgattaa tctaactcga aaaggggtga tactagttca agactcaggc
      901 gagattttcc ttcggataat aatttaatcg atacagaaat aagatagctc tttaatggca
      961 tttgaaattt ctttgcattt cttactcatc ttctaattta aattcgatta aaaaatagat
     1021 aatcacttag tattatgatt gcttaaaagt tgagtaacgt ttaagcaatt ttatccaatt
     1081 agtacaaatt ttaattaaag cgatctttat tatttaacta aaaaccgatt gtgccaactt
     1141 tcatttgcag gcactaacca gctatttttc agctccttgc cactaaacac aagattgttg
     1201 aacacgagtg tgtctgattc aatttgtccg ttcgatgcca aggtttcaaa ttcagccata
     1261 ggatttatct gaagacttcc attatcatct aaccaagcaa tctgcatacg atctagaaaa
     1321 tctattcgaa gttcttggcc aattgcattt atccaactaa ccgatttgtc gattgagcaa
     1381 ccacttgctt ttgcttgtgc ctcatccacc atcaccacaa caaataaatt ccggataact
     1441 aatacatcag cgcgtaaagg tttgccatga gcagcccatc ccgaaacgaa caacattcca
     1501 gattttttga tgtaagtgga ttcctcttcc gacaaaatac ggtttgcttg atacacccaa
     1561 attctatctg tatcccgaat gctttcaaaa gtcttttcca ttttcttata aatctgcagc
     1621 gtttgcaatt agttcagcaa tgtctaaaac actcactgaa gcttcttttt cggcatgttt
     1681 aacgccgtca gtcatcatcg tattgcaaaa cggacaacca actgcaataa tagttggatt
     1741 caaagccaat gcttcttccg aacgttccac atttacttct ttatcacccg gttcggcttc
     1801 tttaaacatt tgcgcaccac cagctccgca acaaagccct ttggttttgc aacgtttcat
     1861 ttctaccaat tcggcatcaa gtttcccgag taccgcgcga ggagcttcat attcgccgtt
     1921 tgcccgaccc aaataacacg gatcgtgaaa tgtgattttc ttccctttaa acgaaccacc
     1981 ttctactttc aatcgaccat ctttcaacaa actctcaatt aattgagaat gatgcaccac
     2041 atcgtaatgt ccacctaact cgggatattc attttttagg gtattaaagc aatgcggaca
     2101 agcagtcacg atcttcttca cctcatatcc attcaaaact gcgatatttt gcatagcctg
     2161 catctggaac aggaattcat ttccagcacg tttggcagga tcgccggtgc agctttcttc
     2221 tgagccgagt actgcaaatt tcacatcagc tgcttgaagc aatttcgcta cagcttttgt
     2281 tactttttta gcacggtcat caaaactccc agcacaacct acccagaaaa gaatttcagg
     2341 aacctcacca tttgcaaggt attgagccat cgtttttaca ttcaattcca tatatgatta
     2401 caaattacgg attaggaatt acggattagg aattacaaaa tacggatgtg acctaactaa
     2461 atgtttaatt gtggagtttg ccttttgtag ttcgttgaat tgagccaatg atacgtaaaa
     2521 tctcatctat ttgaatgagt aaatcttctg aatgtgttgg ttcaatggtg ttagaatctc
     2581 tcagtaatcg aatccaaaat ttagtttctc tcgcctcttt atatgctata ctgattttgc
     2641 tcaaaaaatc ctttctcgat tgaccaccaa tagcttcttc cacattagcc cctattgagg
     2701 ttcctgattt cagaagttgt cgagataaca caaactcttt attcttgttc atttgccttg
     2761 aagtgttaat aatctttagc gcaaaatcat aggttttctg ttgaatcaaa ttctctttca
     2821 tacaatctag tttatattta tagctattat ttaattaatg ttaattcctc attcttaatt
     2881 gaccattcgt aattcgtaat tgaaaattcg taattattac actactcgtc cttccaattc
     2941 aagcgatccg atggagaaaa ctgccaagga gctccattgt tctccacatt ggtaaacatg
     3001 gcactcaatg ctgctggagg agcactttct tccatcacca attgacgacg taaatcgatg
     3061 atgatgctca atggatcgat attcaccgga caagcttctg tgcaggcatt acaagttgta
     3121 caagcccaca attcttctgg actaatataa tcgtgaagca aacttttgtc atcttccttg
     3181 aattcacctt ggttagcatc gatgttttta cccacttcct ctaaacggtc gcgagttttc
     3241 atcatgatag ctcgcggact caataactta cctgtttgat ttgccggaca ttcagaagta
     3301 catcttccac attcggtgca ggtataggca tcgagcaagc ttttccaagt taaatcctga
     3361 acgtcttttg ctccgaactt tactggccca tctaattcaa ccggaacagc cgaaggatcg
     3421 agcatcaatt ttacttcatt ggttaccgct gccatgttgg taaaacgacc tttcttctcc
     3481 aagttcgaat accacacatt gggaaatgcc aaaatgatgt ggaagtgttt ggagtaaggc
     3541 agataattga ggaatgctaa aatcccgaga atgtgaaacc accaagcacc gtgctcatag
     3601 gcgattaaag caccatccga ccaagagcta aacaatcctg taataagcga gcttaccgga
     3661 aaggaacctg cttgaatata atgctcaaca ccacgtgttt gtaatacttg atcagctgca
     3721 ttcatggtta agaaagcagt catcaacaat acttcaatca tcaaaatggt attcgcatca
     3781 gaacgtggcc atccattgag ttctttctga cgaaaacgct tgatatatat aatgttccgg
     3841 cggatgaaga atataattac tgctactaaa acacctaatg caaggaattc aaaggaacct
     3901 atcagaaaat cgtaaaaacc tcctaaaaat ggagcaaaca ggcgatgtgt tcctaaaata
     3961 ccatctagga ttatttcaag aacttccagg ttgataatga caaatcctac atatactaat
     4021 aggtgcaata tagccgcagt tgggcgaacc accattttac tctgtcccaa agctacacgg
     4081 aacataatat tccagcgttc agcttttcta tcggtgcgat cgagttttct tcctaaacgt
     4141 atgttgcgga taatcttgcg cacatttaca gtgaacaaag cagctccagc tagaaagaaa
     4201 aggataaata gtacgttttg caggcccata agcagcaggg atttgaatca acaaatataa
     4261 aggtgaggtg caagcagccc gattattttt tcggcgcttt ctccttcttt ccaaaaacag
     4321 aaaaatgcac gtaacggttc ggatgtgcct tcatatcctc caacaataaa ctcaaattgc
     4381 gagaactatg atccaaattc acatacaaag aatcgtcggt tgccagtttt cctaaagtgc
     4441 cctgaccact ttctacattc tttagaacct tattcaaact tgcaatcgtt tcctgcaaat
     4501 cggttaacgt ttgtttcaca ttactcttcg ccacatcatc cgaaattcga tccatattcg
     4561 tgaaaacatg ggagagtttt tcgttgttgt ttttcagatt atcggtgata gaagccacat
     4621 tactcaatat cgaactcaat ttgtgacgct cattagtcac caataaatca aattcattta
     4681 ccgtgtgttc gaagcgtttg atggatgtat tcacactttc aaaactgcta atcaaaccat
     4741 cctgagtttt acgattgaat acattattaa agcctgttaa aaccgaatca accgaaccag
     4801 ctaaagcttc aatttgtgct tttaatggga ggatagcttt cttaatctca tcggtaattc
     4861 ccgcatcgcg aactgccatt aacgtatcac ctcgttcagc taactgggtt gattttccca
     4921 gcgttagttc caaagttctc gttcccaaca gatcttgaac aatatgcgct tccgaatctt
     4981 tggggatatt caaatcatca ttgtcgatta gaaatttcac caagacttta tacgaacctt
     5041 ccgataaaaa tcctacttga tctacaattc ctacctgaaa tccattcacc tgaacccttg
     5101 ccgatggcat gagttgatct acattgtcga aaaccgcgta atacgatcta gacggggaaa
     5161 aaaggttttt tccttttagg aagttaaacc caaaatagag cagaacgatt cccagtgtaa
     5221 caacgactcc aacacgtatt tcacgggata tttgcatttt tttcaatgat tgtttagggt
     5281 tgaatagctt gtgcttcttg taatgtaatg cgttgattat ttctaaatgc aactacaaat
     5341 gcatcactga aaccatcttt ttttaattcg ctgagcaatt taagggcttc ttcctgtttc
     5401 tcacatcttc caactgtata tttccaggat ttattgaatt tatattccca tacatcggtc
     5461 cgtcctttaa agcgagccga cttcaacgaa actttttcgg gtgatgtata cacttgaatt
     5521 cgataaataa tcccagaatt atcttcagcc gtttgattct ttgcctcttc cgttttttcc
     5581 actttcggat ctggcttaac atgatgttcg gatggtttta cctcttgaac atgctcctgc
     5641 tcttcatcgg gaaacttcag atcgttgtta gcccccgttc cttcaatcca gcttttatat
     5701 gaattcaatg cgcggagtat cgaattggca atcttttgct gccccgttcc cgatttcaaa
     5761 tatttctctt cttccccatt cgttaagaaa ccagtctcaa tcaacacact tggcatagtg
     5821 gttcggtaaa gcacgaggaa tccagcttgc ttaactccac ggttgtggcg tgttgcatag
     5881 gttttaaact catgttgtat gcgatctgcg aaatacaaac tctggtctaa aaatgcattc
     5941 tgatacaaag agaatataat gtttgcttcg gcactgttcg ggtcaaatcc atcgtatttc
     6001 ttttggtaat tatcttcctg aaggattacc gcattctccc gcttcgccac attcaaatta
     6061 tcggtgcttt tatgcaaacc cattacaaag gtttctgctc cgtatgctgt ctttggtcca
     6121 ctgttgcagt gaatgcaaat aaataaatca gccttgtttt catttgcaat ctgtgctctg
     6181 cgatacaatt ccacaaacac atccgtctta cgagtgaaaa ttactttcac atctgggaag
     6241 tttgcttcaa tcaattttcc caactttaat gagacagcta aagcaacagt tttctcataa
     6301 gctgctgctc catgacatcc gctatcgtgt cctccatgtc ccggatcaat cactatcgta
     6361 cgaattccag attcccgatg caagttttta tctgcctcta attttggcag aaaagctagc
     6421 gtaacccctg atatcagaag gattgcgaga aacgtaaatc gatattttaa aaaggttttc
     6481 atgggtcctg atgggcctag aaacgggttt gcgatgaaat aaattagttt tgcactcgtt
     6541 tcgaaacgga ttttacaaaa ctaatcggct gcaaccttgc gattacgtgt ggtgctacgg
     6601 cacttaacaa cagttttatt cacagtattt ctcgggatga gctcaagggc tcaaactcct
     6661 gatagaaacg ttgtggacgc cgattcattg cttgccttgc agctcaagga ggacaccgtt
     6721 cgcaaatctg gctctttacc acccggttct ttgccacctt ctgatgatgc attagactca
     6781 cgtgtcgaat acaaagcccg cgactccatg cgattcgatt taaagttgaa gaaaattttc
     6841 ttgtatggcg ctgcggatgt gaaatacgat aaaattcatc tcactgccga ttacatcgaa
     6901 atcaactggg gaaacaacgt cgttcatgcc gaaggtcgtc ccgattctac agggaaaata
     6961 attggtcatc ccgtgtttga tgaaggaact caaaccttcg acgcggatcg gatggattat
     7021 aatttcaaaa ccaagaaagg tcgtatctct ggaatcttca cccagcaagg cgatggctat
     7081 attcatggtg aacaggtgaa gaaaagtgag aaggatgaat tctacatccg aaatggaaag
     7141 tacactacgt gtaatttacc cgatcatcca catttttaca tcaatgccag taagattaaa
     7201 gtcatgccgg gcgataaaat tgtttctggc ccagctaatc tttggattga agatgttccc
     7261 actcctttag ctgttccttt tggaattttc cccaatagca ccaaacgtaa atcgggaatt
     7321 atttttccaa cctatggcga atctcccgga ctcggttttt acctgcagga aggcggctac
     7381 tattttggaa taaacgatta catcgatgcc tctattcggg gaaatattta ttcccgtggt
     7441 tcttggggat taagtgctgg aacttcctat aattatcgct atcactttaa tggaagttta
     7501 gatgttcgct tttctaaaat cttgcagggc gatcctgaat tattaggctc atccatcaac
     7561 aaagattttt tcatcacctg gaaacatact caagatccta aatcaagacc gaattctcgc
     7621 ttttcggctt ctgttcaagc aggttcgagt aattataaca aactcaactc actcaatgcg
     7681 aataacattg tagctaacac ttttacttcg agtatttcat acgctaaaac gtttgctaat
     7741 actccattta atatggtcat cagtgctggg catagtcaaa acac
//
LOCUS       DAOOQW010000106         7689 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_11734_length_7689_cov_4.857742, whole genome shotgun sequence.
ACCESSION   DAOOQW010000106 DAOOQW010000000
VERSION     DAOOQW010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 7689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7689
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11734_length_7689_cov_4.857742"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(53..760)
                     /locus_tag="PK281_07115"
     CDS             complement(53..760)
                     /locus_tag="PK281_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009414800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOY49153.1"
                     /translation="MGRAFEFRRARKEKRWSKMAKDFVKMGKQLSMAVKQGGPDPETN
                     PTLRVVIQNCKALNMPKDNIESAIKRASAKDAKDLDELVYEGYGPYGVAVLVETATDN
                     PTRTVANVRMYFARAEGALGKTGSLDFIFDRKAVFTLGMEGLDLEEMELELIDFGLEE
                     IFEEDGKAFIYTSFKDFGGMQQELERRKLNIISSELIRLPSTTVELTPDQEEEINTMV
                     EKMEDDDDVLHVFTNMK"
     gene            987..1454
                     /locus_tag="PK281_07120"
     CDS             987..1454
                     /locus_tag="PK281_07120"
                     /inference="COORDINATES: protein motif:HMM:NF017897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF962 domain-containing protein"
                     /protein_id="HOY49154.1"
                     /translation="MRTIQNWLDEYSESHQNPLNKTIHWICVPSIVFSIVLMLWSIPA
                     PIWILNTGLNYAYFAMILAVIYYALLSPTLAIGMALLFALAGFIAQAIALSTNYPLWG
                     IGLILFVLAWIGQFYGHSVEGKKPSFLKDIQFLLIGPVWLLHFIYTRLGIKYN"
     gene            1503..2228
                     /locus_tag="PK281_07125"
     CDS             1503..2228
                     /locus_tag="PK281_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49155.1"
                     /translation="MISGCKKDEPVFEVDPCLSASPKEAFLSFSMYVDSLPFALQTDY
                     LLNDGHHVKFETAQFYLSGLHLLKDSNQINLSNDVALITPTSLQTNLGKIEQSSYTGI
                     KFDFGIDSLRNHGDPTLFSAGNPLAIQVPSMHWSWNQGYIFAIFEGKFSTTTINSSNP
                     GNDFSYHIGLDSNFKPDNLRSFTIPLNVSACGDNNIKLRFNAAHVFNGLTLETDFNTQ
                     STVNYGLSTRVRNNIISGITSVQ"
     gene            2233..3009
                     /locus_tag="PK281_07130"
     CDS             2233..3009
                     /locus_tag="PK281_07130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49156.1"
                     /translation="MKKTLFLPLFVLSIFLISCEKDESVVSETPEKTTGTVEIRFIPT
                     MNGEAFSVNQNFIGPNGKRMRYETFKFFLSDIQLQNGSTVLGSKDIALVDFSLSDKTI
                     TIETTPGTIDQLNFGLGVKQSLNGTADPDFNPAAYPMDHPLSVYSGMYWTWASGYIFS
                     KLEGRIDTSAAQNQDPIYSYFYHSGKDTLYATHSISNINAVVVKGQKTNINLKLEVND
                     VFKTSTDTINMVQDYFTHTTDHFDLAQKVINNLGDAIRKQ"
     gene            3010..4029
                     /locus_tag="PK281_07135"
     CDS             3010..4029
                     /locus_tag="PK281_07135"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HOY49157.1"
                     /translation="MMFRAFLYSIICIALVSCAKDDAIADKDVYPFKTPTGFSEPYIP
                     DHNPLTYSKIKLGKKLFYDPILSSDGSISCNSCHKQSLAFSDARTVSLGVGNLPGFRN
                     AQPLFNLAWSSSFFRDGGVTILELVPLNAIDSHVEFNNNVGEIIRSLNKSAEYRTLFK
                     IAFNDTANTNNFLQALACFQRILVSADSPYDHFHFANETKALSESAKRGMELFNSSKT
                     DCFQCHSGFNFTNSEFLSNGISETYPDSGRQRITLQLADKGKFRVPSLRNIEYTAPYM
                     HDGSFETLNDVIEHYNSGGKNFSGKSPLIRPLGLNEQEKNDLVEFLKSLSDTKFIQNK
                     EFYKE"
     gene            4038..5093
                     /locus_tag="PK281_07140"
     CDS             4038..5093
                     /locus_tag="PK281_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HOY49158.1"
                     /translation="MKRLLILLCVCSTLFSSCKKDEDEPETPYQTTPYTLETPPGFPA
                     MAIPADNPITVEGVALGRKLFYEKLLSGNNTQSCATCHNQALSFTDNGTRFSTGITGA
                     VGTRNAQPLINLGYNLHYFWDGRAGTLEDQALGPVKNPIEMHLAWTEAAARLNANTSY
                     KAEFKKAFNVDVIDSTHVVKALAQFMRTMISYNSKLDRRLRNEANLSASELNGYVIYN
                     TEKGDCFHCHSIDAGRLLTDNQFHNNGLDSIFTDLGRGAITGNPNDNGKFLTPTLRNI
                     ALTAPYMHDGRFQTLEQVIDHYNTGGKASSTVDPLMKHVGTGLNLTSQEKADLLAFLR
                     SMTDSTFINDTRFKSPF"
     gene            complement(5094..6788)
                     /locus_tag="PK281_07145"
     CDS             complement(5094..6788)
                     /locus_tag="PK281_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49159.1"
                     /translation="MNKFLLILVLLLPVVGFSQPGKSNNDVVVTGGYTPKLIDANKIN
                     EQPAIADTGIVRFPIQYELNSRRVNTVYEIDPIKAANVKGEPLSKLYRGYVKGGFGSY
                     SMPYGEIFYHNLRSKKSSAGVHYKHLSSSGQISNVGYSGFSSNELNLFGKSFIKKSTL
                     SGGLDYRRNVVHYYGFDKAVAYNQDSTDNPYWGYNLDKDSIRQRYQLVAVNASFSDNY
                     PVDSHATKYKVDLNYYNYADHYKAQENAFKAAGDVSFYYKTYDLNIKSGLDVYKNQNA
                     AWTDNMTIFQLRPQITFSQPKWRLKAAINMYATSDSSNGFKLAPEVDFDLHLYENIII
                     LNAGTDSRLQRYSYRSLTERNPWLISDILPLNTWSPFRLYAGLRGSISSKMAFNTRVS
                     YTPSIDNQFFFVNDTSAGNLNKLTYVTDNASLLEVNGEITWQMHEKIRIIAKADFLGY
                     TPDKELKAWHVPTLRMSYGGKYNLKDKIWINAAVLTYNKQYYRDFIVDDNTGEIKTVA
                     KELKGIVDVNLGAEYRYTKLLGMFVQLNNIINVRYERYKNYPMQRFNVMAGVSYIF"
     gene            complement(6781..>7689)
                     /locus_tag="PK281_07150"
     CDS             complement(6781..>7689)
                     /locus_tag="PK281_07150"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOY49160.1"
                     /translation="DCDLAMGQMSSYLRDFPEGLFSLQALGYKADCEMKARKVDEAAL
                     TYGQILLRPKNKYTAEAHLILGIYERQKDNFPKAIEHFKELEKMPDNSDQLLQARSNV
                     MRLSARLGNTAESEEYAKKVLETEKVDEDMKNEAKLILARAELKSENWDAALSDFQKL
                     RKVNSEIGAEARYSIALIYFNKGDYKHCEKSVFDLVDEMPSYDDWLAKGFILLADNYL
                     KLGNVFQAKRTLQSVIDNHEGQELRDLAQHKLDEIISSENSGNSNEKQVDPDIEFKNN
                     SNDNGLFDEQKAPVEGQEPKKGDDHE"
BASE COUNT         2319 a         1644 c         1282 g         2444 t
ORIGIN      
        1 aagacagtaa tatcaaagac ttccgaagta aagttgtaat tagccgataa gcttatttca
       61 tgttagtgaa cacatgaagc acatcgtcat cgtcttccat tttctccacc atggtattaa
      121 tctcctcttc ttgatcagga gttaattcaa cggttgtact cggtaaacga atcaattcgg
      181 aactgatgat gttgagtttt ctacgttcca actcttgttg cattcctcca aaatctttga
      241 aagatgtata gatgaatgct ttcccgtcct cttcgaaaat ttcttctaaa ccaaaatcaa
      301 ttagctcgag ttccatttcc tccaaatcaa gtccttccat tcctaaagtg aaaacggctt
      361 tacgatcaaa aatgaagtcg agcgatccgg tttttcctaa cgcaccttca gcacgtgcaa
      421 aatacattcg cacattcgca acagttcggg taggattgtc tgtagcagtt tcaactaata
      481 cagcaactcc ataaggaccg tatccttcat atactaattc atccaagtct ttcgcatctt
      541 tcgcagaagc tcgtttgata gcagactcaa tattgtcctt aggcatattc agagccttgc
      601 aattttgaat cactacgcga agtgttggat tggtctccgg atcaggtcca ccctgtttta
      661 ctgccatcga caattgcttg cccatcttga caaaatcctt agccattttg ctccagcgct
      721 tctctttgcg cgcacgacga aattcaaatg ctcttcccat gttgaaattt tatttccgat
      781 ctaattcggt ttcactttcc tgttgggatg gtaggattta accttctgac acagtaaatt
      841 taataactaa actgagccac aacccgaaaa atgaggtgtc aaaactaagg attttttcag
      901 caaagcaaaa tgcttgattg ccttccaaga ttaattctca gcccgtttag ctttttaata
      961 acttgcacat taaaccgatt caaaagatgc gaacgatcca aaactggctg gatgaataca
     1021 gcgaaagcca tcagaatccg ttaaataaaa caatacactg gatttgtgtt ccctccattg
     1081 tgttcagtat tgtcctcatg ctctggagca tacctgcacc tatttggatt ctaaacacag
     1141 gtttaaacta tgcctatttt gcgatgatac ttgctgtaat ctattacgct ctattatcgc
     1201 caaccttggc tattggcatg gctttattat tcgcacttgc tggatttatt gcacaagcca
     1261 tagcactttc tactaattac ccgctttggg gaattggact tatcctattt gtccttgcat
     1321 ggattgggca gttttacggg cattctgttg aaggtaaaaa accatctttc ttaaaagaca
     1381 ttcaatttct tctcattggc cccgtttggc ttttacactt tatatacacc cgtttgggaa
     1441 tcaaatacaa ctaataaatg aagtttcctt ttttatctgt ttgcatactt gtctgcgtca
     1501 gcatgatttc aggctgtaaa aaagatgaac ccgtttttga ggttgatcct tgcttgagtg
     1561 catcgcccaa agaagctttc ctgagtttct cgatgtatgt tgatagcttg ccatttgcac
     1621 ttcaaacgga ttatttactc aacgatggac atcatgttaa attcgaaaca gcacagtttt
     1681 atctctcagg attacattta ttgaaagaca gcaaccaaat taatttgtcc aatgatgtgg
     1741 cactcattac gccaacatct ttgcaaacga atctgggaaa aatcgaacaa tcaagctaca
     1801 ccggaataaa attcgatttt ggtatcgact cgcttcgaaa tcatggtgat ccaactttat
     1861 tcagtgccgg aaatccactg gcaattcagg ttccatccat gcattggagc tggaatcaag
     1921 gctatatttt cgctattttt gaaggtaaat tctcaaccac cacaatcaac tcttctaatc
     1981 cgggtaatga tttctcctat cacatcggtt tagatagcaa ttttaagcct gacaaccttc
     2041 gttcttttac aattccattg aatgtttcag cctgtggaga caataatata aaactccgat
     2101 ttaatgcggc acatgtattt aatggattaa cacttgaaac agatttcaac acgcaatcaa
     2161 ccgtcaatta tggattatcg actagagttc gtaataacat catttcagga attacttcag
     2221 ttcaatagta tcatgaaaaa aacactgttt cttccgctct tcgtcctttc aatctttctc
     2281 atttcatgcg aaaaagatga atcagttgtt tctgaaacac ctgaaaaaac tacgggtacc
     2341 gttgagattc ggtttattcc aacaatgaat ggcgaagctt tttctgtaaa tcaaaatttt
     2401 attggtccta atggtaaacg tatgcgttat gagacattta aatttttctt gtctgatata
     2461 caactacaaa acggatcaac ggtcctaggc tctaaagaca tcgctttagt tgatttttcc
     2521 ctttctgaca aaaccataac tatcgaaaca acaccaggaa caattgacca attaaatttt
     2581 ggattgggtg ttaaacaatc tcttaatggt actgccgatc ctgattttaa tccagcagct
     2641 tatccaatgg atcatccact aagcgtttat tctggtatgt attggacttg ggctagcggg
     2701 tatattttct caaaactcga aggccgaatc gataccagtg ctgcacaaaa tcaagatccc
     2761 atttactctt acttctacca ttctggaaaa gacacactat atgcaacgca cagtatttcc
     2821 aatattaatg cagttgttgt gaaaggtcaa aaaactaaca ttaatctaaa attggaagtc
     2881 aatgatgtat tcaaaacatc aaccgacaca attaatatgg ttcaagatta ctttactcat
     2941 acaactgatc acttcgattt ggctcaaaaa gtcatcaata atttgggtga tgcaatcaga
     3001 aaacaataga tgatgtttcg ggcttttctt tattccatca tttgtatagc cttggtttca
     3061 tgcgctaaag atgatgctat tgctgataaa gatgtttacc cgtttaaaac tccaactgga
     3121 ttctctgagc cttatatccc agatcataat ccactcacct attccaagat caaacttggc
     3181 aagaaactct tctacgatcc gattctatcc agcgatggaa gtatttcctg caactcgtgt
     3241 cacaaacaaa gtctcgcatt tagtgatgcc cgaactgtta gtctaggagt tggaaattta
     3301 cctggatttc ggaatgccca acctttattc aatttagctt ggagttcgag ttttttccgt
     3361 gatggaggcg ttaccattct cgaattagtt ccactcaacg ctatcgattc gcatgtagaa
     3421 ttcaataata atgtgggtga aattattcgc agtttgaata aatcagctga atatcgaacg
     3481 ctatttaaaa tagcatttaa cgataccgca aacaccaata attttctgca ggcattagct
     3541 tgtttccaac gtattttagt tagcgccgat tctccatacg accacttcca ttttgcaaat
     3601 gaaaccaaag ccttaagtga atcagctaaa cgtggaatgg aattatttaa tagcagtaaa
     3661 acggattgtt ttcaatgtca ctccggattt aatttcacga attcagaatt cctttccaat
     3721 ggaatttccg aaacgtatcc cgattctggc cgccaacgca taactttgca actcgccgat
     3781 aaaggaaaat tcagagtgcc atcattaagg aatattgaat acactgctcc ttatatgcac
     3841 gatggtagtt ttgaaacctt aaacgacgta attgaacatt ataattcagg tggtaaaaac
     3901 ttttcaggca aaagcccgtt aatccgacca ttaggattga atgaacagga aaaaaatgat
     3961 ttggtagaat ttctcaaaag tctgtctgat actaaattca tccaaaacaa agagttctac
     4021 aaagagtaaa tagtattatg aagcgcttgc tgattctttt gtgtgtttgt tctacattat
     4081 tttcctcttg taagaaagat gaagatgagc cggaaacacc ttatcaaaca acgccttata
     4141 cattggaaac tcctccagga tttccagcaa tggctatacc tgccgataat cctatcacag
     4201 ttgaaggtgt tgctttgggc agaaaacttt tttacgaaaa acttctttcg ggtaacaata
     4261 cgcaatcgtg cgccacttgt cataatcaag cactttcgtt taccgataat ggcactcgat
     4321 ttagcacagg aataacgggt gcagttggta ctcgtaatgc tcaaccactt atcaatctcg
     4381 gatataatct ccattatttc tgggatggca gagctggtac tctagaagat caagctcttg
     4441 gtccagttaa aaatccaatc gaaatgcatt tggcttggac tgaagccgct gctcgtttaa
     4501 atgcaaatac atcctacaaa gctgaattca aaaaagcttt taatgtggat gttattgact
     4561 ctactcatgt ggtgaaagct cttgctcaat tcatgagaac tatgatttcc tataactcta
     4621 aattggatcg tcgtcttcga aatgaagcca atctttctgc gtccgaactc aatggatatg
     4681 taatttacaa caccgaaaaa ggagattgct ttcattgcca tagcatcgat gcaggtcgtt
     4741 tacttaccga caatcagttt cacaacaatg gactcgattc gatcttcacc gatttaggac
     4801 gtggagctat caccggaaac cccaatgata acggcaaatt tctaactcca acacttagaa
     4861 acattgccct tacagctcca tacatgcacg atggtcgttt ccaaacactc gagcaggtta
     4921 tcgatcatta caatactggc ggaaaagcat cctctactgt tgatccactt atgaaacacg
     4981 ttggtactgg tctcaatcta acttctcagg aaaaagccga tttgctggca ttccttagat
     5041 caatgaccga tagcactttc atcaacgaca ctcgtttcaa aagtccgttc taatcaaaag
     5101 atatagctaa ctccagccat cacattgaag cgctgcatag gatagttttt gtatcgctca
     5161 tatcgaacgt ttataatgtt attcaattga acaaacattc ctaaaagctt cgtgtagcga
     5221 tattcagctc ctaaattcac atcaacaatt cctttgagct cttttgcaac agttttgatc
     5281 tctcccgtat tatcatctac tataaaatca cggtagtact gcttattata agtcaataca
     5341 gctgcattta tccagatttt atctttcaaa ttgtacttcc caccatacga catacgtaaa
     5401 gttggaacat gccatgcttt caattcctta tctggcgtat agcctaggaa atcagcttta
     5461 gcaataattc gaattttttc atgcatttgc caagtgattt caccattcac ttctagtaaa
     5521 ctagcattat cagtgacata tgttagctta ttcaaattcc ctgcgctcgt atcattcaca
     5581 aagaagaatt gattgtcgat gctcggtgtg tatgaaacac gagtattgaa ggccatttta
     5641 gaactaatcg aaccgcgcaa accggcatat aatctaaatg gactccaagt atttaatggc
     5701 aaaatatccg aaatcaacca aggattcctt tcagttaaac tacgataaga ataacgctgc
     5761 aaacgactat cagtacctgc atttagaatg atgatgttct catataaatg caaatcgaaa
     5821 tcaacctctg gagctaactt aaatccattg gaagaatcgg aagtagcata catgtttatt
     5881 gcagctttca agcgccattt aggttgacta aatgtgattt gtggacgcaa ctgaaaaatg
     5941 gtcatgttat ctgtccaagc tgcattttga ttcttataaa catccaatcc tgatttaata
     6001 ttcaagtcgt atgttttgta atagaatgat acatcaccag cagctttgaa agcgttttct
     6061 tgggctttgt aatgatcagc atagttgtaa taattcaagt caaccttata ctttgtagca
     6121 tggctatcca caggataatt atcactaaaa gatgcattta ctgcaaccaa ttgatagcgc
     6181 tggcgaatac tgtctttatc caaattataa ccccaatatg gattgtctgt tgaatcttga
     6241 ttataggcaa cagctttgtc aaaaccataa taatggacca catttcgacg gtaatccaaa
     6301 ccacccgata aggtcgattt cttgataaaa gatttaccaa aaagattcag ttcattggaa
     6361 ctaaatccgg aataaccaac attgctaatt tgtccagatg atgaaagatg cttgtaatga
     6421 acaccagccg agcttttctt cgatcttaaa ttgtgataaa aaatctctcc gtaaggcatc
     6481 gagtagctac caaatccacc tttcacataa ccgcgatata attttgaaag cggttcacct
     6541 ttgacatttg ctgctttaat aggatcaatt tcataaacgg tattcacgcg tctactattc
     6601 aattcatatt ggataggaaa acgaacgatt ccggtatcag cgattgctgg ctgttcatta
     6661 atcttattgg catctatcaa tttgggagta tatccacctg taacaacaac atcattgtta
     6721 ctttttcccg gttgagaaaa tccaaccact ggcagaagca atacgagtat taacaaaaac
     6781 ttattcatga tcatctcctt tcttaggttc ttgcccttct actggtgctt tctgctcatc
     6841 aaacaagcca ttatcatttg agttgttctt gaattcaata tctggatcta cttgcttttc
     6901 gttcgagttt ccagaatttt cagaacttat aatctcatct aatttgtgtt gtgccaagtc
     6961 acgtaattct tgaccttcat gattatctat aacgctctgc aaagtccgct tagcttggaa
     7021 aacattgccg agtttcaaat aattgtctgc caataaaata aatcctttcg ctaaccaatc
     7081 atcatacgaa ggcatttcat caactaagtc aaaaaccgat ttttcacagt gcttgtaatc
     7141 acctttattg aaatagatta gtgcaattga ataacgagct tctgctccaa tctcactatt
     7201 tacttttctc agtttctgga aatcactcaa agcagcatcc caattttccg actttaattc
     7261 agctcgagcc aaaatgagtt ttgcctcatt tttcatatcc tcatctacct tctccgtttc
     7321 gagtactttt ttagcatact cttccgattc agctgtattt cctaatcgag cagataagcg
     7381 cattacatta cttcttgcct gtaacagctg atctgaatta tcaggcattt tctctaattc
     7441 cttaaaatgc tcaattgctt tagggaagtt gtccttttgt cgttcgtaaa tccctaaaat
     7501 caaatgggct tctgccgtat acttgttctt aggtctcagc aagatttgac catacgtcaa
     7561 ggcagcttca tcaactttgc gggctttcat ctcacaatcg gccttatatc ctaaagcttg
     7621 taaagagaat aatccttcag ggaaatctct cagataagaa ctcatttgcc ccattgctaa
     7681 atcacaatc
//
LOCUS       DAOOQW010000107         7677 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_11751_length_7677_cov_5.343873, whole genome shotgun sequence.
ACCESSION   DAOOQW010000107 DAOOQW010000000
VERSION     DAOOQW010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 7677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7677
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11751_length_7677_cov_5.343873"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(97..2118)
                     /gene="uvrB"
                     /locus_tag="PK281_07155"
     CDS             complement(97..2118)
                     /gene="uvrB"
                     /locus_tag="PK281_07155"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HOY49161.1"
                     /translation="MDFQLISDFSPTGDQPEAIRQLTEGVERGDKSQVLLGVTGSGKT
                     FTVANVIQQTGRPTLILSHNKTLAAQLYQEFKTFFPKNAVEYFVSYYDYYQPEAYLPV
                     TNTYIEKDLQINDEIEKLRLSTASSLLSGRRDIIVISSVSCIYGMGNPENIKSNTVKI
                     KRGEIISRNRFLHLLVAALYSRTEAEFKNGTFRVKGDTVDVFIGNASVAYRFYFFGDE
                     IEQIEIIDPESGSRLEEPDQVVIYPATIYVANKETMKDAIYLIQEDMVKQCEFFKEEG
                     KYLEAKRLEDRVTYDLEMIRELGYCSGIENYSRYFDGRIKGTRPFCLLDYFPDDFLMV
                     IDESHVTLPQIKAMYGGDRSRKVNLVEYGFRLPAAMDNRPLQFDEFTALVNQLIYVSA
                     TPAEYELNQSEGIVVEQLIRPTGLLDPIIEVRPSQNQIDDLLEAVDKVVKRDERVLVT
                     TLTKRMAEELSKYMTRLNIRCRYIHSDVDTMERVEILGDLRLGKFDVLIGVNLLREGL
                     DLPEVSLVAILDADKEGFLRSVRSLVQTVGRAARNVNGRVIMYADKITDSMAQTIDET
                     NRRREKQMKYNIEHDITPTQIVKSHDSIFAKRSGGSSSQSSPRAYVENESIDLAADPV
                     VKYMSKEQLQKSIAQTRKSMEKAAKELDFLEAARLRDELFALEKLLKEK"
     gene            complement(2152..2490)
                     /locus_tag="PK281_07160"
     CDS             complement(2152..2490)
                     /locus_tag="PK281_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOY49162.1"
                     /translation="MAVKNDEDNEKLFYTIGEVAARFDVNASLIRFWEKEFDSIKPQK
                     NKKGNRLFTKEDLNNFHLIFHLVKERGYTLQGAKQKMKQNNGELEQSHEIVKNLQRVR
                     SFLVELKNNL"
     gene            2739..4013
                     /locus_tag="PK281_07165"
     CDS             2739..4013
                     /locus_tag="PK281_07165"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOY49163.1"
                     /translation="MSQTTTLTKTRHEQVLDTIDEVTRISSNLGIAQLQIEDEDINGR
                     TIRVKGKEVVNFGSCSYLGLEKHPAIIEGVVQAVTKYGSQFSSSRAYASVTLYTEAER
                     LLEAMFERPILLAPTVTLGHLSNIPVLVGDRDCVILDLQVHSCVQTAAQLIKARGIHV
                     ELIRHNRMDILEERINELKDKYEKIWYMADGVYSMYGDFLPLNELYQLLDTYEQFHLY
                     VDDAHGTGWAGKNGSGYVLSQKPFHERLYLVAGLAKSFAACGGVLVFPNEQIKRRVRN
                     CGGTFIFSGPIQPPMLGAIIASAKLHLTPEIKTHQAELQKRIDYFVETCKSFKLPLIG
                     ESNSPIFFIGVGKPGVGYNMVTRIMNKGFYINLSVFPSVPYKNTGLRVPLTTGHTFED
                     IYNLLSVIAEQLPFALKDNNSSMEEINRAFKI"
     gene            complement(4069..>4491)
                     /locus_tag="PK281_07170"
     CDS             complement(4069..>4491)
                     /locus_tag="PK281_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOY49164.1"
                     /translation="KSLDKFRATRELSGTNKQATEVQEKITEELSLNDRIVVKNGADI
                     RILPLSEIRYLEAYDDYVKIYTRTGMFLKKQTLGYFERKLHASSFLRIHRSYLLNLSE
                     LTRIEPAEKDQHQAILKDKTRLPLSRSGYARLKEELGL"
     assembly_gap    4492..4501
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4502..4812)
                     /locus_tag="PK281_07175"
     CDS             complement(<4502..4812)
                     /locus_tag="PK281_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOY49165.1"
                     /translation="MIRVLLIDDEPLARQLVRKYLQTQTDVEVLAECGDGFEAAKAIA
                     QHKPDLIFLDVQMPKISGFELLELLDPAPAVIFTTAFDEFALKAFEANAIDYLLKPFP
                     A"
     gene            complement(4815..5831)
                     /locus_tag="PK281_07180"
     CDS             complement(4815..5831)
                     /locus_tag="PK281_07180"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOY49166.1"
                     /translation="MKNSPVLFPASIGWLIWVSLHLVFLQQHQVPLLPAIQDAVLFAS
                     ILFLSAASISRVLKYYKPDWKKAIGFVVGNAAVATGVMSLYSWTANYFLKPGQKLTDL
                     LPNAEVVLFTLSFLFLLCTGLIHWLWLTLKEREDDTKRKSDQETLARNAELNNLRQQL
                     QPHFLFNSLNSIQALMELDPEKAREMLRLLSDFLRGTVKKEPTQLVSFEEEIKQVRLY
                     LAIESIRFSDRLNIDWSVDDNTLSHKVPVLILQPIIENAVKFGLYGTTGPIRIRISAR
                     MNGDVLEVSTQNPFDSDTGTNQGTGFGLSSVARRLYLLYARNDLLSSKKENQQFIITI
                     RIPS"
     gene            complement(5832..6200)
                     /locus_tag="PK281_07185"
     CDS             complement(5832..6200)
                     /locus_tag="PK281_07185"
                     /inference="COORDINATES: protein motif:HMM:NF025484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4288 domain-containing protein"
                     /protein_id="HOY49167.1"
                     /translation="MKTYLARLVFQVDWEPQQTNTAFEEQIRLIIAEDKLGAYTQANA
                     CGHTEEGEFPFYRGGSVNWKFLGLTSLDEIGQIHSGMLIDSTQRNQVNSVEDGKFLQA
                     QIQRFNNQLEVETMQKHRIL"
     gene            complement(6203..7027)
                     /locus_tag="PK281_07190"
     CDS             complement(6203..7027)
                     /locus_tag="PK281_07190"
                     /inference="COORDINATES: protein motif:HMM:NF021443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LiaF-related protein"
                     /protein_id="HOY49168.1"
                     /translation="METTSSPENPINNDMPPFNPPGTNKTGRVLGGIVIVGVGALLFA
                     RQAGVFFPEWLFSFETFLIALGFYIWARHEFRRPGGLILMAFGGLLLIDNIIPNLYIG
                     QFFWPLIIIIGGLWMILSPGKHSWSTRRMNRRARWKDNRYGQKAGYSTGFTSSSTDPK
                     DEAYLDSTAIFGAVKKNFFTKDFKGGEVVNFMGGTEINLSQAEIQQPVTLEISQVFGG
                     TKLILPAHWQVRSEIIAIMGGVEDKRAIHPSVQEDPNRVLILRGTTVFGGIDIKSY"
     gene            complement(7069..7494)
                     /locus_tag="PK281_07195"
     CDS             complement(7069..7494)
                     /locus_tag="PK281_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49169.1"
                     /translation="METINTETQINNEVPYCKPRQKSRLGRILGGLVIIAAGGFLFAR
                     NAGVVFPEWLFSGPMFLFTLGLFFLAKNAFRRPGGLILMTIGGFLLLGRIFPALLIGQ
                     FFWPVFIIIAGAFMIFKPKRKHHWQYQMHHQQRWEQCRR"
BASE COUNT         2304 a         1508 c         1540 g         2315 t
ORIGIN      
        1 cagaatttaa attgataaca gatagcgcag tattatttgc ttgtctggtt ttacacgaaa
       61 accaaatcag taaagacatg ataccgacca agaatttcat ttttccttga gtaatttctc
      121 gagtgcaaat aattcatctc gtaaacgagc agcttcaagg aaatcaagct ctttagcagc
      181 tttctccata gacttacgcg tttgagcaat tgatttttgt aactgttctt tgctcatgta
      241 tttaacaacc ggatcagcag ctaaatcaat tgattcattt tctacataag ctcgaggtga
      301 ggattgagaa ctacttccgc cacttcgctt cgcaaaaata ctatcgtgcg atttcacaat
      361 ttgtgtaggt gtgatatcgt gttcgatgtt gtatttcatt tgtttttcgc gacgacgatt
      421 ggtttcatct atcgtctgtg ccatcgaatc ggtgatttta tctgcataca taatcacgcg
      481 accattcaca ttacgagcag cacgaccaac agtttgtact aatgaacgaa ccgagcgcaa
      541 gaatccttct ttgtcagcat ccaaaatagc taccagtgaa acttccggta aatcgagtcc
      601 ttcacgtaat aagtttacac caatcaatac atcaaacttc cctagacgta aatcccccaa
      661 aatttctaca cgctccatgg tgtcaacatc cgagtgaata taacgacaac ggatatttaa
      721 gcgtgtcatg tatttcgata attcttctgc catccgttta gttagcgtgg tcactaaaac
      781 acgttcgtca cgttttacaa ctttatcgac cgcttctaat aaatcgtcaa tttggttttg
      841 actcggacga acttcaataa ttggatcgag caatccagtt ggacgaatga gttgttcaac
      901 cacgatacct tcactttggt tgagttcgta ttcggcaggt gtcgcactta catatataag
      961 ttgatttacg agtgcagtaa attcatcgaa ttgtaaaggt cgattgtcca tagcagccgg
     1021 caagcggaaa ccatattcta cgagattcac tttccgagaa cggtcgccac catacatggc
     1081 tttgatttgt ggaagtgtca catggctttc gtcaatcacc atcagaaagt catcggggaa
     1141 ataatccagc aaacaaaacg gacgtgttcc tttaatacgt ccatcaaaat agcgggaata
     1201 attctcgatt ccactacaat agcctaattc acgaatcatt tcaaggtcgt acgttacccg
     1261 atcttcgaga cgttttgctt cgaggtattt tccttcttcc ttaaagaatt cgcattgttt
     1321 caccatatcc tcttgaatga ggtaaatggc atctttcatg gtttctttat tcgctacata
     1381 aatggtagct ggataaataa ccacctgatc gggctcttct aaacgagagc cactttcggg
     1441 atcaatgatt tcaatttgtt caatttcatc cccgaaaaaa taaaagcgat acgcaaccga
     1501 tgcattacca ataaatacat ctactgtatc accttttact cggaaagtac cgtttttgaa
     1561 ttcggcttcc gtgcgcgaat agagagctgc aacgagtaaa tgcagaaagc gattccgact
     1621 aataatttca ccccgtttta ttttaacggt attggattta atgttttccg gatttcccat
     1681 accgtaaata caggaaacgg aagaaatcac aattatatct cgacgtccag ataggaggga
     1741 agaagcagta cttaaacgta gtttttcaat ttcatcattt atctgcagat ctttttcgat
     1801 gtaggtattg gtgactggca gataagcttc tggttgataa taatcgtaat acgaaacgaa
     1861 atactcgaca gcatttttgg ggaagaatgt tttaaattct tggtagagct gagcagcaag
     1921 ggttttatta tgactcagaa taagtgttgg tcgacccgtt tgttggatga cgttagctac
     1981 agtaaaagtt tttccagaac cggtaacacc aagtagaact tgagatttat cgccccgttc
     2041 aacgccttcc gtcaattggc gaatggcctc gggctgatca cctgtaggag agaaatcgga
     2101 aataagctgg aaatccattt tatgtattca aataaaagcg atagatgctg attaaaggtt
     2161 gttttttaat tcaaccaaaa aagagcgaac acgttgaaga tttttcacga tttcgtgact
     2221 ttgttctaat tcaccgttat tctgtttcat cttttgtttg gctccttgaa gcgtgtaacc
     2281 acgttctttt accaaatgaa atatgaggtg aaaattattt aaatcttctt tggtgaaaag
     2341 gcgatttcct tttttgtttt tctgcggttt aatgctgtca aattcttttt cccaaaaacg
     2401 aatcaaggaa gcatttacat caaagcgcgc ggcaacctca ccaatggtgt aaaaaagttt
     2461 ttcgttgtct tcgtcgtttt taactgccat agcgcaaaga tagaaaagcg tgttctattt
     2521 acagtgtgga tgtttgagta gaattgaaat gaattaaaaa gaaattaatg aattaaatat
     2581 tcctagaatt ttaggggtat tggttttcaa aaattcatgg aaatagttga aaatagaggt
     2641 aaagtatgtt gataaacctg tctatatctt taaggattat ttcataaaaa aaaggaattc
     2701 acttggttaa atttgggaaa cgcacacaca acctgaccat gagccaaacg actacactaa
     2761 caaagacccg acacgaacaa gtgttggaca cgattgatga agtaactcga atatcatcta
     2821 acctcggaat tgctcaatta caaattgaag acgaggatat caacggacga acaatccgtg
     2881 tgaaagggaa agaggtcgtt aattttggat catgcagtta tttaggattg gaaaagcatc
     2941 cagcaataat tgaaggagta gtgcaagcag ttactaaata cggctctcaa ttttcatctt
     3001 ctagagctta tgcttcagtt accttgtata ccgaagctga gcgtttatta gaagctatgt
     3061 ttgagcgacc tattttgctt gctccaacag taacacttgg acatctttcc aatataccgg
     3121 tattagtggg cgatcgggat tgtgtgatat tggacttaca ggtgcattca tgtgttcaaa
     3181 cagcagctca acttattaaa gctcgtggaa ttcatgtgga gttgattcgt cacaaccgaa
     3241 tggatatttt ggaagaacga atcaatgagc taaaagataa atacgagaaa atttggtaca
     3301 tggccgatgg cgtgtattcg atgtatggtg attttttacc gctaaatgaa ttgtatcaac
     3361 tgttagatac ctatgagcaa tttcatttgt atgtggatga tgcgcacggt actggctggg
     3421 cagggaaaaa tggttcagga tacgtgttaa gtcagaaacc attccatgaa cgactttacc
     3481 ttgttgctgg attagcaaag agttttgcag cctgcggtgg agttttagtt ttcccgaatg
     3541 agcaaattaa acgtcgtgtg cgtaactgtg gtggaacgtt tatcttttct ggacctatac
     3601 agccaccgat gttaggtgct attatcgcat ctgcaaaatt acacttgact ccagaaatca
     3661 aaacacatca agcggagctt caaaaacgta tcgactattt cgtagaaact tgtaaaagct
     3721 tcaaacttcc attaattggt gaatcaaatt cccctatttt ctttatcgga gttggaaagc
     3781 caggtgtagg ctacaatatg gttacacgca ttatgaataa aggtttttat atcaacctga
     3841 gtgtattccc aagtgtgcct tataaaaaca ctggattacg tgttccatta actacgggac
     3901 atacattcga agatatctac aacttacttt cagttatagc cgaacaattg ccatttgcat
     3961 tgaaagacaa taattcgagc atggaagaaa ttaatcgcgc ttttaaaatc tgataggtca
     4021 tccacctaca agccgcaatg gagtaatctg ttgcggtttt tttatggatt aaagtcccaa
     4081 ttcttctttt aaacgagcat atccgcttct actgagtggc aatcgagtct tatcttttaa
     4141 aatggcttga tgttgatctt tctcagctgg ttctattcga gtcagctcgc tcaggttgag
     4201 caaatacgaa cgatggattc gaagaaatga cgatgcatga agtttgcgtt caaaatatcc
     4261 aagcgtttgc ttctttagaa acatccctgt tcgcgtgtag atttttacat aatcgtcata
     4321 agcttctaaa tacctgattt cggataaagg tagaatacga atatcagctc catttttcac
     4381 tactattcga tcgttcaagg ataactcctc agtaatcttc tcttgaactt ctgtggcttg
     4441 cttattcgta ccacttaatt cacgtgttgc gcgaaactta tcgagtgatt tnnnnnnnnn
     4501 ntcagccgga aatggcttca ataaataatc aatcgcatta gcttcaaacg ctttcagggc
     4561 aaattcatca aaagcagtag tgaaaataac ggctggtgcc ggatctaaca attcgagcag
     4621 ttcaaacccc gaaatcttag gcatttgcac atccagaaaa atcaaatcgg gtttatgttg
     4681 tgcaatcgct ttagccgctt caaatccatc accacattct gccaaaactt cgacatctgt
     4741 ttgggtttgc aaatactttc taaccagctg acgagcaagt ggttcatcgt cgattaataa
     4801 aacacgtatc atacttatga tggtattcga atggtgatga taaattgttg attctctttc
     4861 ttgctactca acagatcatt tcgcgcataa agcaaataaa gtcggcgtgc taccgaactc
     4921 aacccaaagc cagttccttg attggttcct gtatctgaat caaatggatt ttgagtcgaa
     4981 acttctaaca catctccatt catcctagct gagatacgaa tacgaatggg gccagtagtt
     5041 ccatacaatc caaacttcac agcattctca ataataggct gtaatatcag gacgggaact
     5101 ttatgactta acgtattgtc atccaccgac caatcaatat tcaatcgatc cgaaaaacga
     5161 atactttcaa tggcgagata taaacgaact tgtttgattt cctcttcaaa agaaaccaac
     5221 tgagttggtt ctttcttaac tgttccgcgc aagaaatccg acaataaacg caacatttca
     5281 cgcgcttttt ccggatctaa ttccatcaaa gcctgaatcg aattgaggct gttgaataag
     5341 aagtgtggtt gaagttgctg acgaagatta ttcaactcgg catttcgtgc taatgtctcc
     5401 tgatcagatt tacgttttgt atcatcttcc cgttctttta atgtaagcca taaccagtgg
     5461 ataagtccag tacataaaag gaatagaaag cttaaagtaa ataagacaac ttctgcattt
     5521 ggaagtaggt cggtgagttt ttgtccaggt tttaggaaat agtttgctgt ccaggaataa
     5581 agcgacatca cgccagttgc caccgctgca ttaccaacaa caaacccgat ggcttttttc
     5641 caatcgggtt tatagtattt gagaacacgg ctaatactgg cagcactcag aaatagaata
     5701 ctagcaaaaa ggacagcgtc ttgtatggca ggtaaaagcg gtacctgatg ttgttgcagg
     5761 aaaacaaggt gcaaactaac ccaaataagc caaccaatac tggcaggaaa caagacgggc
     5821 gaatttttca tttagagaat tcgatgcttt tgcatggttt ctacttcaag ttgattgttg
     5881 aaacgttgga tttgtgcttg aaggaatttt ccatcttcca cgctattcac ctggtttcgt
     5941 tgagtggagt cgattagcat accggaatgg atttgtccga tttcatcgag tgaagtcaat
     6001 cccaaaaact tccaattaac cgatccgccg cgatagaaag gaaattctcc ttcttcggta
     6061 tgtccgcatg catttgcttg tgtatatgca cccagtttat cttcagctat aatcaagcga
     6121 atttgttctt cgaatgctgt gttcgtttgt tgcggttccc agtctacttg gaaaactaat
     6181 cgagctagat acgttttcat gcttagtaac ttttaatatc tatccctccg aaaacagtcg
     6241 ttccacgtaa aatgagtacc cgatttggat cttcttgtac actcggatga atagcacgtt
     6301 tatcttctac acctcccatg attgctataa tttcagaacg tacttgccaa tgtgccggta
     6361 agatgagttt ggttccacca aatacctgag agatttcgag tgttacaggt tgttgaattt
     6421 cagcctgact gagattgatc tccgttccac ccataaaatt caccacttca ccacctttga
     6481 aatctttcgt gaagaagttc tttttcacag caccaaaaat ggcagtagaa tccaaataag
     6541 cttcgtcttt gggatcagtc gaacttgatg taaatccagt ggaatatcca gctttttgtc
     6601 cgtatcggtt atctttccaa cgtgctctgc gattcatgcg tcgtgttgac caagaatgtt
     6661 tgccgggact taatatcatc caaagtccgc caatgatgat gattaaaggc cagaagaatt
     6721 gtccgatgta caagttggga atgatattat caatcaatag caatccaccg aaagccatta
     6781 gaattaaacc accggggcgg cgaaactcgt gacgagccca gatataaaat ccgagtgcga
     6841 ttaagaatgt ttcgaatgag aataaccatt cggggaaaaa tactcctgct tgacgagcaa
     6901 agagtagtgc acccacacca actatgacaa ttccgccaag tacacgacct gttttattgg
     6961 ttcccggagg attaaaagga ggcatatcgt tgttgatagg attttcgggt gatgaagtag
     7021 tttccataat agtaaatcgt tagaaatcgt ttgtaaagct ggaatcgatt atcggcgaca
     7081 ctgttcccag cgttgttgat ggtgcatttg atattgccag tgatgcttac gttttggctt
     7141 gaatatcatg aatgctcctg cgatgataat gaataccggc caaaagaact gaccaatgag
     7201 taaggcaggg aatattctac caagaagtaa aaatccacca atggtcatga ggattaatcc
     7261 acctgggcga cgaaaagcat ttttagctag gaagaaaagt ccaagtgtaa agaggaacat
     7321 gggtcctgaa aagagccatt cgggaaatac tacgccagca ttacgagcga ataaaaatcc
     7381 accagcggct atgatgacta atccaccgag gattctacca agtcgagatt tttgacgcgg
     7441 tttacagtaa ggtacttcgt tgtttatttg agtttctgtg ttgatcgttt ccatgttgtt
     7501 tgttttgatg aggcaaacgt acgcggagga ttttgggtga gaaaccagaa ttcggtgaat
     7561 ggtataaaag ggtaggtgaa tgacgaaaaa atcgatatca accgaaattc tcattagtta
     7621 gccgattgac gtaatcgttc accgatagat tggaagagta atacagtgat gaaaatg
//
LOCUS       DAOOQW010000108         7632 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_11833_length_7632_cov_4.384057, whole genome shotgun sequence.
ACCESSION   DAOOQW010000108 DAOOQW010000000
VERSION     DAOOQW010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 7632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7632
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11833_length_7632_cov_4.384057"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(194..1087)
                     /locus_tag="PK281_07200"
     CDS             complement(194..1087)
                     /locus_tag="PK281_07200"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOY49170.1"
                     /translation="MLSVLIPVYRYDVRDLVNELHRQCTALSIPFEIRLLDDGSGDKF
                     CQTNKVVEQLPNVVWTELAKNSGRSAVRNTLAKSALYEWLWFLDCDGDARVNPSLART
                     FWEEKDANTLVSGGRIYQAKPPENHTLYLHWLWGSQRELLDPEIRMKDPVTTFLSNNF
                     FLHRSLLERVPFDNYFHGYGYEDTFFAAELIQAGYKIKHIKNPVLHAGLENAKDFLKK
                     IEESVDNLSRLKDRCEEKNIPFPVKSKLMKAYRVLNFPLVRPLFAKWFSRNLPYWKIQ
                     LLGKTPDLKTFDLYRLGFLLD"
     gene            complement(1089..1769)
                     /locus_tag="PK281_07205"
     CDS             complement(1089..1769)
                     /locus_tag="PK281_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007092732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOY49171.1"
                     /translation="MGLDTTISLKNVNIYQHENLVLHEVNLEIEKGEFVYLIGKTGSG
                     KSSLLKTLYADLGLKEGEGEVVGYDLKTIKTKDIPFLRRKLGTIFQDFQLLSDRSVEE
                     NLRFVLQATGWKNNGEMDERVDDVLKKVGLPTKKHKFPHQLSGGEQQRVGIARALLND
                     PELILADEPTGNLDPETTAEILQLLLDIAANGRSVLVATHDLTLFELHRARTLKCDGG
                     QILDSNLL"
     gene            complement(1772..1972)
                     /locus_tag="PK281_07210"
     CDS             complement(1772..1972)
                     /locus_tag="PK281_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49172.1"
                     /translation="MHILKIKGKAKIPDYIQIRDDDFTLKAYFRVDRPDKSLEKMGLG
                     NRMEELLKLIEEMPFGKVIKID"
     gene            complement(2240..>2428)
                     /locus_tag="PK281_07215"
     CDS             complement(2240..>2428)
                     /locus_tag="PK281_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49173.1"
                     /translation="VAITAQVGYDKMGDVMKWVKDSRYEYDNPVMENQFSITIHVQAR
                     RVDEVKNLFVDNKWMDAD"
     assembly_gap    2429..2528
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2529..2994)
                     /locus_tag="PK281_07220"
     CDS             complement(<2529..2994)
                     /locus_tag="PK281_07220"
                     /inference="COORDINATES: protein motif:HMM:NF013378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HOY49174.1"
                     /translation="MKSDDHYRTIAAPSEGNFRDRGSKFLGYAFPVSNEEQIKENLQN
                     LRKLHPSARHVCYAFRINPLNEYWRANDDGEPSSSAGKPILGQIRSMELQNVLVAVVR
                     YFGGTLLGVPGLINAYREAAVEALQAAEIVEKIVTVAITAQVGYDKMGDVMKW"
     gene            complement(2991..3884)
                     /locus_tag="PK281_07225"
     CDS             complement(2991..3884)
                     /locus_tag="PK281_07225"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOY49175.1"
                     /translation="MIYLLLSILSSAALMIVFKLYDRFKVNTSDAIVINYLVAASLSF
                     ILDPTGLAKNGASSQPWFYHAMVMGILFISLFNVIGISTQKIGVSVTSVANKMSLIMP
                     VLFAIFFLNDSVNAVKIIGIIIALLAVYFTTRSEERTDIDKRYALFPLIVFIGSGFID
                     TFFKYNEEFTLGENGLEPFLAWIFLTASIIGVVILIINYIRHKKLPALNAYIGGLFLG
                     IPNYFSVFFLVKSLSIPSMESSVVFPVNNMSIVAVSALAGIFFFKEKVSRINIIGIVL
                     CMLAIACIAFSEDILPMFGIE"
     gene            4251..4760
                     /locus_tag="PK281_07230"
     CDS             4251..4760
                     /locus_tag="PK281_07230"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY49176.1"
                     /translation="MTKLLLSVTLLLPIFSYSQTTDRNVVSSAGSYVVDAESGISFSQ
                     TIGENIVFTSQNPSFTFTQGFQQPDVDWNASIETQFADQFESSVWPNPFENQLSLAIK
                     SSRNTNLVVSFSDLAGRKISSDQAIQVSSTLTTWNIAVENLAAGIYLIQIRNKQGELI
                     TTHRVNKSH"
     gene            4790..>7632
                     /locus_tag="PK281_07235"
     CDS             4790..>7632
                     /locus_tag="PK281_07235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49177.1"
                     /translation="MKKLSFSALVFGMLLFAATASKAVAQDVPRGINYQAVARTASGQ
                     LMANQNLSIRISLLSPSSTNQLVYEEVQSSIQTNQYGLFNLVIGQGVPSGTGAISFSL
                     VDWSIPDIMIKIEADLGSGYVDMGTTSLWSVPYALHAANGTPGPQGEVGPQGPAGADG
                     ATGVQGPQGEQGIQGPQGVDGAQGPIGLTGPQGEVGPQGPAGAAGADGLNGLDGATGP
                     QGPQGIAGVDGAQGPQGEIGPQGPAGLNGLDGATGPQGPQGIAGVDGAQGPQGEVGPQ
                     GPAGADGLNGLDGATGPMGPQGPAGLNGLDGATGPQGPAGADGLNGLDGATGPMGPMG
                     PMGPQGPQGISGGLAWTSDRPYVAGEVVVYQGETYICQFNNDGEPGGIFPTDETYFAP
                     YSLVGPQGPQGEMGPQGPTGSSSANVLTGVVAVENGGTGMDLLGGANQQIRVNADGSA
                     LEYFTPSITGVQNLNGATETNQSLTTGNAGNDFNISTDAGVHTLNIPDAGVSSRGLIT
                     NGSQIIAGDKTIVNPLQINNNLFLATTDGSPGYHLATDGENLAISHTGVMDNQLYLQS
                     NGDVQVRNNLLVSGSGQTGGGLILADDGDMVDMNNGYATMRFSSGVQITNGNRSGDPV
                     ITLSSNGNVYSNAFIKTNGTGNQFLKADGSVDETSYATANDLTNLSSNYLPLSGGTLS
                     GNLSGTSSGFSTLTLSQSGNTPFLNLPSEMTMSTTDQGDLVLRNLDQLRFTDANDWDY
                     SSWAGLAYKRANNQVVLGFPDGNNFLGGNNGTLLFSNVNEAYMGNTDNVVIHSGNYQS
                     KISSLYSLSLTQTGATPFLNLPSEMTMSTTDAGDLVFRNLDQLRFTDANDWDYSSWAG
                     LAYKRATNQIVLGFPDGNNFLGSTTGTLFFSNVNNAYIGNTNQEIIHTGNLSQFALPK
                     PADAGVQGQILSLSIDGTTAWVNTRTEVADEVLA"
BASE COUNT         2236 a         1543 c         1483 g         2270 t
ORIGIN      
        1 gtaaactttt aactgaaatg gaagccttat aatttgattt attgctgatt tcaaaagggt
       61 cttcaactag ttcatatgtg aataatacca gaatctattt gctcaacaat tcgtaaatat
      121 tgctaaaatc tcctgtaggt attaaaagga agattagata attggttgag ttggcaagtt
      181 tcagaaaatg tattcaatcc agtaaaaaac ccaatcgata caaatcgaat gttttcaagt
      241 caggagtttt accgagtaat tgaattttcc aataaggaag attcctgcta aaccatttag
      301 caaaaagtgg acgaactagt gggaagttca gaactcgata agctttcatg agtttgctct
      361 tgaccggaaa cgggatgttt ttctcttcgc agcggtcttt tagtcgactt aaattatcga
      421 ccgattcttc aatttttttg aggaaatctt tcgcgttttc taatccagcg tgtaggacag
      481 gatttttaat gtgcttgatt ttataaccag cttgtatcaa ttctgcagca aagaaagtgt
      541 cttcataccc ataaccatga aagtaattgt caaatgggac gcgttctaat aagcttcgat
      601 gaaggaaaaa attattgctc aggaaggttg ttaccggatc tttcattcga atttccgggt
      661 ctaataattc gcgctgagaa ccccaaagcc agtgaagata taacgtgtga ttttcgggtg
      721 gtttggcttg ataaattctt ccgccactta ccaaggtatt tgcatccttt tcttcccaaa
      781 atgtgcgagc caaactagga tttacccgag catcaccgtc acaatcaaga aaccaaagcc
      841 attcgtatag ggcagatttc gctaaagtat ttcgaactgc agatcgacct gaatttttag
      901 ctaattcggt ccatacaaca ttcggtagtt gttctactac tttattggtc tgacaaaatt
      961 tgtcacccga tccatcatcc aacaaacgaa tttcgaatgg gatagaaaga gccgtgcact
     1021 gacgatgtaa ctcattcacc aaatcgcgca catcgtaccg ataaaccgga atgagtaccg
     1081 aaagcatctt agagtaggtt ggaatcgaga atttgtccgc cgtcgcattt taacgtacgg
     1141 gcacgatgaa gttcgaatag tgtaaggtcg tgcgtagcaa ctaaaactga gcgaccatta
     1201 gcagcaatat caagtaataa ttgaagaatc tcggcggttg tttcggggtc gagatttccg
     1261 gttggctcgt cggctaggat gagttcggga tcattcaata aagcacgggc aattcctaca
     1321 cgttgttgtt ctccacccga taattgatgg ggaaatttat gttttttggt aggcaatccc
     1381 acttttttaa gcacatcatc cacacgttca tccatctctc cattattctt ccaacctgtt
     1441 gcttgtagca cgaatcgtag gttttcctct acacttcgat cagatagtaa ttggaaatcc
     1501 tgaaatatcg ttccgagctt gcgccttaaa aatggaatgt ctttggtttt gattgttttc
     1561 aaatcatatc caaccacttc accttcacct tctttcaaac ctaagtcggc ataaagcgtt
     1621 ttcaataagc tactttttcc gctgccggtt tttcctatca gatagacgaa ttcgcctttt
     1681 tcgatttcca gatttacttc gtgaagtacc aggttttcgt gctgataaat atttacgttt
     1741 ttaagtgaaa tggtggtgtc caaacccatg ttcaatctat tttaatcact ttgccaaaag
     1801 gcatttcttc aatcaatttt aacaactctt ccatgcggtt gccgagcccc attttttcta
     1861 atgatttatc gggacgatct actcgaaagt atgctttaag tgtaaaatca tcatcgcgga
     1921 tttgaatgta atccggaatc ttcgctttac cttttatttt gagtatatgc atgtcgttag
     1981 ctgctgggac gaatataggg aattgaaatg agggcttagt tcctgtcaat aaattttagg
     2041 aacaagaatt ctctaataac acgtatctaa tcataatgtt ctttgtactg atcgctaata
     2101 acggggcaaa ttcttagctt gcgcctatga atgctttcaa agatttctgg aaaaaatact
     2161 ttccctattt tcaggttgat atgctttact atgctttggt aatcctactg gtaccaatca
     2221 ttttgcttat ctctcgctgt taatcggcat ccatccattt attatctaca aataggtttt
     2281 ttacctcatc cactcttcga gcctgaacat gaatggtaat gctaaattga ttttccatga
     2341 ctggattatc atattcgtat cggctatcct ttacccattt catcacatct cccattttat
     2401 catatccaac ctgagcggtg atggcaacnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnncc catttcatca catctcccat tttatcatat ccaacctgag cggtgatggc
     2581 aacggtgact attttttcta ctatttctgc agcttgcaaa gcttctacag ccgcttctcg
     2641 ataggcatta attaatcccg gaacaccaag taaagttcct ccgaaatacc tcacaacggc
     2701 aactaaaaca ttctgcaatt ccatcgaacg aatttgtccg agaatgggtt tccccgctga
     2761 agaagacggt tcgccatcat cattcgcacg ccaatattca tttagtggat tgatgcgaaa
     2821 ggcgtaacaa acatgtcgag ccgaagggtg aagttttcta aggttttgca ggttttcttt
     2881 tatctgctct tcattcgaaa cgggaaaagc ataacctaaa aacttacttc ctcgatcgcg
     2941 aaaatttcct tcgctcggag cagctatcgt tcggtaatga tcatccgatt tcattcgatt
     3001 ccaaacatcg gaagaatatc ctccgaaaag gcaatacatg caatagctag catgcataac
     3061 acaatgccga taatattaat tctggatacc ttttccttga aaaagaaaat cccagctaag
     3121 gctgatacag ctacaatact catgttattt acagggaaaa ctacagagct ttccatactt
     3181 ggaatgctga gtgatttcac caagaaaaat accgagaaat aattggggat acctaagaat
     3241 aatccaccaa tataagcatt caatgcaggt agttttttgt ggcggatgta gttaataata
     3301 agaatcacta caccaattat agaagctgtt agaaaaatcc aagcaagaaa tggttctaat
     3361 ccattttcac ctaaggtgaa ttcttcgtta tacttgaaaa atgtatcaat aaagccactt
     3421 ccaataaaaa caattaatgg aaataatgca tatcgcttat ctatatcggt gcgttcttca
     3481 cttcgtgtgg taaaataaac ggccaataat gcgatgatga ttccaattat tttaactgca
     3541 ttcacactgt catttaaaaa gaaaatcgca aataaaacgg gcattatcag cgacatttta
     3601 ttggcaaccg aagtcactga aactcctatt ttttgagtac taataccaat cacattaaat
     3661 aaggatataa acaagattcc catcaccatg gcatggtaaa accagggttg agaagaggct
     3721 ccgtttttag ctaaacctgt tggatctagt ataaaactca aacttgcagc tactaagtaa
     3781 ttaatcacaa tggcatccga agtattcacc ttaaaacgat cgtatagctt gaatacaatc
     3841 ataagtgctg ctgaactgag tatgctgaga agtaagtata tcatcgtcta tcttgaaccg
     3901 cgaaaatagg cattcaagtc tgtatttgag tgttttctta tttgttccaa ttattcactc
     3961 ttgaaattga gcgattcaat ccgtaaaatt taaaatcttc ttagtacaga attaagtgaa
     4021 taaaaacact tagtgaggaa tccccatttt atgaaaattc tggtttcaaa atagacatct
     4081 cttacaattc aaaaagagat ttaaattatc tctaatagcc tatttcattg tgtttgttaa
     4141 tttatttcaa acacaacaac cgtatattct tacatgtgtc agatataacg cattaggtcc
     4201 ttcgcatcca agttccttca tacacctttg gatcaaccaa atgctaacaa atgacaaaac
     4261 tcctactttc tgttacatta ttactcccaa tttttagtta ctctcaaacg actgatcgga
     4321 atgtagttag ctccgccggt tcttatgtag tagatgctga atcgggaatt tcgttttctc
     4381 aaacgatagg cgaaaacatc gtctttacta gccaaaatcc ttctttcact tttactcaag
     4441 gatttcagca acctgatgtt gattggaatg catcaattga gactcaattt gctgatcaat
     4501 ttgaatcatc cgtatggcct aatccattcg aaaatcaatt gtcacttgct atcaaaagtt
     4561 cacgaaatac caatctggtg gtttcgttta gcgatttagc cggacggaaa attagttccg
     4621 atcaagccat tcaggtctca tccacattaa caacttggaa tatcgctgtc gagaacttgg
     4681 cagcaggtat ttacctcatt caaatccgga ataagcaagg tgaattaatc accacacacc
     4741 gggtaaataa atctcattaa aattttacca atccaacccc taaaagaaga tgaagaaact
     4801 ctccttttca gcacttgtat ttggcatgct cctgtttgcc gcaactgcat caaaagctgt
     4861 cgcgcaggat gttccgcgtg gtattaatta tcaagccgtt gctcgaactg catccggaca
     4921 actaatggcc aatcaaaact tgagtattcg gatttcactc ctaagcccga gctcgaccaa
     4981 ccaattagtt tacgaagaag ttcaatcgag cattcaaaca aatcaatatg gtttgttcaa
     5041 tttagtcatt ggacaaggtg tgccatcagg tactggtgca attagctttt ctttagttga
     5101 ttggtcaatt cctgatatca tgataaaaat cgaagccgat ttaggttccg gttatgtcga
     5161 tatgggaacg acttctctct ggtctgtacc atacgcatta cacgccgcaa atggcactcc
     5221 tggtccacaa ggtgaagtag gtcctcaagg tcctgctggt gctgatggag caactggtgt
     5281 gcagggacca caaggagagc aaggtattca aggcccacaa ggtgtcgacg gtgctcaagg
     5341 tcctatcggt ttaacaggtc cacaaggtga agttggtcct caaggccctg ctggagctgc
     5401 tggtgctgat ggacttaatg gcttagatgg tgctacaggt cctcaaggtc cacaaggtat
     5461 tgctggtgtc gacggtgctc aaggtccgca aggagaaatt ggtccgcaag gtccagctgg
     5521 tttaaatggt ttagatggag caactggtcc acaaggtcca caaggtattg ctggtgtcga
     5581 cggagctcaa ggtccacaag gtgaagttgg tcctcaaggc cctgctggtg ctgatggtct
     5641 taatggttta gacggtgcaa caggtccaat ggggcctcaa ggtcctgctg gacttaatgg
     5701 tttagatggt gctactggtc cgcaaggtcc cgctggtgct gatggtctta atggtttaga
     5761 cggtgcaaca ggtccaatgg gtccaatggg tccaatgggt ccgcaaggtc cacaaggtat
     5821 ttcgggtggt ttagcttgga caagtgatcg tccttatgtg gctggtgaag tagtcgttta
     5881 tcaaggcgaa acatacatct gtcagttcaa taatgatgga gaaccaggtg ggattttccc
     5941 aacggatgaa acgtattttg ctccttattc attggttggt ccacaaggac cgcaaggtga
     6001 aatgggacca caaggaccaa ctggttcatc aagtgccaat gtacttacag gtgttgtggc
     6061 agttgaaaat ggcggtaccg gaatggatct attaggtgga gcaaatcaac aaattcgagt
     6121 aaatgcggat ggatctgctt tagagtattt tacaccatcc attacaggtg tacaaaacct
     6181 aaatggtgct accgaaacga accaatcatt aacaaccgga aatgctggaa atgatttcaa
     6241 tattagtacc gatgcaggtg ttcatacttt gaatattccc gatgcaggag tttctagtcg
     6301 tggtttaatt acaaatggct cacaaataat tgctggagat aaaaccatcg tcaatccatt
     6361 gcaaatcaat aacaatctat ttttagcaac taccgatgga agtccaggct accacttggc
     6421 tacagatggc gagaacctag ctatcagtca cactggagta atggacaatc aattatatct
     6481 tcaatcaaac ggtgatgttc aagtgcgaaa taacttgctt gtttcaggct ctggacaaac
     6541 aggtggcgga cttatccttg ctgatgatgg tgatatggtt gacatgaata acggatatgc
     6601 taccatgcgt ttctcgtctg gtgttcaaat tacaaacgga aatcgtagtg gagatccagt
     6661 aattacactt agttcaaacg ggaatgttta cagtaatgca ttcatcaaaa ctaatggtac
     6721 tggtaatcaa ttcttaaaag ctgatggtag tgtagatgaa acttcttatg caactgcaaa
     6781 cgatctaacg aatctaagtt ctaattatct gccattaagt gggggaacat taagtggaaa
     6841 tttatcagga actagcagtg gttttagtac actaacctta tctcaatctg gtaatactcc
     6901 atttttgaat ctaccaagcg aaatgaccat gagtactacc gatcaaggtg atttagttct
     6961 ccgaaatctt gatcagcttc gctttaccga tgcaaacgat tgggattata gttcatgggc
     7021 aggattagca tacaaacgtg ctaacaatca agtagtgctt ggttttcctg atgggaataa
     7081 ttttctaggc ggtaataatg gtacgctctt atttagtaat gtaaatgagg catatatggg
     7141 aaatacggat aatgttgtaa tccattcagg taactatcaa agtaaaattt ccagcctgta
     7201 cagtctttct cttactcaaa ctggagccac tccctttttg aatctaccaa gtgaaatgac
     7261 catgagcaca accgatgctg gcgacttagt attccgtaac ctcgatcaac tccgttttac
     7321 cgatgccaac gattgggatt atagctcttg ggctggatta gcctacaaac gtgcaacaaa
     7381 ccaaattgta ctcggtttcc ctgatggaaa taacttcctt ggtagcacaa caggtacact
     7441 cttcttttct aatgtgaaca acgcttacat cggaaatacc aatcaagaaa taattcacac
     7501 cggaaactta tctcagtttg ctttacctaa acctgccgat gccggtgtac aaggacaaat
     7561 tctttcgctt tcaatcgacg gaactactgc ttgggtaaat actcgaactg aagttgccga
     7621 tgaagttctt gc
//
LOCUS       DAOOQW010000109         7526 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_12079_length_7526_cov_3.647303, whole genome shotgun sequence.
ACCESSION   DAOOQW010000109 DAOOQW010000000
VERSION     DAOOQW010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 7526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7526
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12079_length_7526_cov_3.647303"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2081
                     /locus_tag="PK281_07240"
     CDS             <1..>2081
                     /locus_tag="PK281_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49178.1"
                     /translation="ALAQGGWQKIYPPILGGVNGDGIEAVRQTADGGFILAGVSEYTA
                     SGGNNRIVKVDDLGNIQWTQSYPNMGMLSSANNIEITSDGGYIIEGTRTNPSTNASEV
                     YLQRIDENGNQLWINIYPEAQNSYSGDLTSDGGYIQVGYYDNNSIQDTLVIVKTAANG
                     TIDWLRKYPNTGTGIERIPIAIMEANNGDLIMMGYKDIGFGGNNFLWRLNASGDSLWQ
                     QSYGIQSNYPEALGGLVELSDGSIVVASNDALSFGNTNVFMFKTTSGGTLIWERHFPQ
                     TNAFGSDIDVTSDGGFIITGYQTIGGSNRILLAKTDNTGNQLWTKFYQGTGAGSWKSF
                     SVRQTTDGGYIVGGAKIQSAYTRTNMYLIKTDDLGEIYGNTLRGYVFADSNADCANTS
                     GEHPFANWMVKASGLQTFYTSTDTNGHYWMRVDTGDYQVTLHPAADNIYWSTSCTNDT
                     LQISIPQNLTTIDTSFAQTTESFCPLLTVDMGTTFLRRCFDNTYMIHYCNTGTADATN
                     AYIDATFDDFIIVNATSIQVPYTQIDSVTYRFPIGDIAIGECGSFAVPVTVSCSTYIG
                     QTHCSQVEIFPNQSCLLPEWTGPSVDLNAECRNDSAIFILHNSNGAMQVAQSYVIYAD
                     TLIAQTGSVLLQSGETLEIGYSASIGSAYRMEVNQAVSYPAYLGDTIVSIAIAACGDS
                     STGAWITQFDN"
     assembly_gap    2082..2181
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2182..2999
                     /locus_tag="PK281_07245"
     CDS             <2182..2999
                     /locus_tag="PK281_07245"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY49179.1"
                     /translation="AACGDSSTGAWITQFDNYDGAPSLDIDCRMNIGAYDPNDKTPSP
                     QGVGEEHFIYDNTELEYLIRFQNTGTDTAFTIVVRDTISDYLDLTSIQPGASSHSYTW
                     RSYGDNVQAVEFTFNNIQLPDSFVNEVASHGFIKYRINQKRSNPLGSIIQNTAAIYFD
                     FNEPVFTNTTFHTIGEDFLESIVITAISENATNENRVKIYPNPFTSLTTFEFPNSAPS
                     QLVISDALGRTVKSLNTNSDKIQLSSEGLANGLYFYRVSSNGKQIDAGKLIIQ"
     gene            complement(3151..5286)
                     /gene="pnp"
                     /locus_tag="PK281_07250"
     CDS             complement(3151..5286)
                     /gene="pnp"
                     /locus_tag="PK281_07250"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOY49180.1"
                     /translation="MNIGVRKTFAMPDGKEITIETGKLAKQAHGSVVITMGGTMLLAA
                     VVSNEDAKEGVDFLPLTVEYQEKYGSTGRFPGGFFKREARPSENEILICRLVDRALRP
                     LFPEDYHSDTTITINLISSDRTQLPDSLACLAASAALAMSNIPFNGPVSEVRVARVEG
                     KLIINPTIDELKNADLDIIVAASTDSIVMVEGEMKEVQEHDMLEAIKLAHEAIKVQIN
                     AITELAALVPASQPKRVYSHETHNEEVRAAVHTFCYDKCVEIARSGNADKHARSAAFK
                     AVKEEFVASLSEEELAVAKPFIGVYFHDVEKKAVRQVILNEGKRLDGRNTTTIRPIWG
                     EVDYLPFAHGSAIFTRGETQSLTTVTLGTKMDEQTIDGAVLEGKSNFLLHYNFPSFST
                     GESRPNRGTSRREVGHGNLALRALKPMIPQGTDNPYTIRVVSDILESNGSSSMATVCA
                     GTLALMDAGVKIAKPVSGIAMGLITDDGSKYAVLSDILGDEDHLGDMDFKVTGTRDGI
                     TACQMDIKVQGLSYEIMLNALNQAKEGRLHILGEMNKVLTAPREDYKPHAPRIVEIRI
                     PKEFIGAVIGPGGKIIQEMQKDTQTVIVIEEVNEQGVVQISSANKANIDKALARIRAI
                     TATPEVGEVYSGKVKTIMPFGAFVEIMPGKDGLLHISEVEHRRLENLDGILKEGDIVD
                     VKLIEVDKKTGKLKLSRKVLLPKPEAAAN"
     gene            complement(5353..5622)
                     /gene="rpsO"
                     /locus_tag="PK281_07255"
     CDS             complement(5353..5622)
                     /gene="rpsO"
                     /locus_tag="PK281_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009414989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOY49181.1"
                     /translation="MALTSELKKEIFTKFGGSETNTGSTEAQIAMFTQRISDLTEHLK
                     VNKKDFGTQRSLIRLVGKRRSMLDYLKARDIEKYRAILKELNIRK"
     gene            complement(5753..6634)
                     /gene="accD"
                     /locus_tag="PK281_07260"
     CDS             complement(5753..6634)
                     /gene="accD"
                     /locus_tag="PK281_07260"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019387901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HOY49182.1"
                     /translation="MSWFSRKNKNITTSTLEKKETPEGLWYKCPSCKKIFASIDHEAN
                     LWVCSNCGFHERVNSREYFSVLFDDNKYEEILNNIRSADPLVFEDTKKYKDRLVASMA
                     KSGYKDAISVGVGPVDGVKMVIACMNFEFIGGSMGSVVGEKISRSIDYSIKHKLPFMI
                     ISKSGGARMMEAAYSLMQMAKTSAKLTQLSDAGLPYISLLTDPTTGGVTASYAMLGDL
                     NIAEPGSLIGFAGPRVVKETIGKDLPEGFQTAEFVLEHGFLDLIIERRDLKFKIAQIV
                     RLLMKQNCTPVVKNAFS"
     gene            complement(7064..>7526)
                     /locus_tag="PK281_07265"
     CDS             complement(7064..>7526)
                     /locus_tag="PK281_07265"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOY49183.1"
                     /translation="LNNYLQGGKSAEAEKLLAIAIEKDPTNHLLHYASGTIFEDLGKR
                     EQAIAAYKKAIEIKPDFWEGYFNLGALYNNEAKRLQDIANNEKDNKKYEAGKKLAEAE
                     FNQALPNLEKALELAPKDSPDLQPLLKTLKQIYSLLNMTDKYNEMKKRLGQ"
BASE COUNT         2260 a         1468 c         1396 g         2302 t
ORIGIN      
        1 gctcttgctc aaggcggatg gcaaaagata tatcctccaa ttttgggcgg cgttaatggt
       61 gatggaatcg aagccgttcg tcaaacagcc gatggaggat ttattctagc gggcgtgagt
      121 gaatacactg cttcaggtgg aaataatcga attgtaaaag tggatgattt gggtaacatt
      181 caatggactc agtcatatcc taacatgggc atgctttcga gtgcaaacaa tattgaaata
      241 acttcagatg gaggttacat catcgaagga actcgcacta atccatcaac aaacgctagc
      301 gaagtgtact tgcaacgaat tgatgaaaat ggaaatcaat tgtggataaa tatttaccca
      361 gaagctcaaa actcatatag cggagatttg actagtgatg ggggatatat tcaagttggt
      421 tattatgaca acaatagcat tcaagatact cttgtcattg tcaaaaccgc tgcaaatggt
      481 acgattgatt ggttaagaaa ataccccaat actggaaccg gaatcgagcg aattccaatt
      541 gctataatgg aagccaacaa cggtgactta attatgatgg gttataaaga tattggtttt
      601 ggtggcaaca atttcctttg gagattgaat gcttcaggag attcattatg gcaacaatcc
      661 tatggtatcc aatctaacta tcctgaagct ttgggaggat tagttgaatt atctgatgga
      721 agtattgtag tcgctagtaa tgatgcatta agctttggaa atacaaatgt atttatgttt
      781 aaaacgactt caggcggaac gctaatttgg gagcgccatt ttccacaaac aaatgcattt
      841 ggatccgata tcgatgtaac cagtgatgga ggatttatca tcaccggcta tcaaacaatt
      901 ggtggttcaa atcgcattct acttgctaaa accgataata ccgggaatca actttggact
      961 aaattttatc aagggactgg agcaggtagt tggaaatcgt ttagtgttcg acaaacaacc
     1021 gatggaggat atattgttgg aggtgcgaaa attcaatcag catatactcg aacaaatatg
     1081 tatttaatta aaacagatga tttaggtgag atttacggga atacattgcg aggatatgta
     1141 tttgctgatt ccaatgctga ttgtgcaaat acttctggag aacatccgtt tgccaattgg
     1201 atggtaaaag ccagtggatt acaaacattc tatacttcaa cagatactaa tggacattat
     1261 tggatgcgtg ttgatactgg tgattatcaa gtaacacttc atccagctgc tgataatatt
     1321 tattggtcta cttcctgcac aaacgataca cttcagattt ccattccaca aaatctaaca
     1381 accattgata catcttttgc ccaaacaaca gaatcatttt gccctttact cactgttgat
     1441 atgggaacta cttttctaag aagatgtttc gataatacct acatgattca ttactgtaac
     1501 acaggaactg ccgatgcaac taacgcctac atagacgcca cattcgacga ttttataatt
     1561 gtaaatgcca cttccattca agttccatat actcaaattg attctgttac ctatcgcttt
     1621 ccaattggtg acattgcaat cggagaatgc ggttcttttg ctgttcctgt tactgtttcg
     1681 tgtagcactt atattggaca aactcattgc agtcaagtcg agattttccc aaatcaaagc
     1741 tgcttattac cagaatggac tggtccttca gtggatttga atgctgaatg tagaaacgat
     1801 agtgccattt tcattcttca caatagcaat ggcgccatgc aagtagctca atcctatgtg
     1861 atttatgcgg acactttaat tgctcaaaca ggaagtgtgt tattacaaag tggtgaaaca
     1921 cttgaaattg gttattctgc aagtataggt tcagcgtatc gaatggaagt taatcaagca
     1981 gtttcttatc cagcatactt aggagatacg attgtgtcta ttgctattgc agcatgtggc
     2041 gatagtagca caggagcttg gattactcaa tttgataatt annnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nttgcagcat gtggcgatag tagcacagga gcttggatta
     2221 ctcaatttga taattatgat ggagctccat ccttagatat cgattgtaga atgaatattg
     2281 gcgcatatga tccaaatgat aaaacacctt caccacaagg agttggcgaa gaacatttca
     2341 tttatgataa caccgagcta gaatacttga ttcgttttca aaacacagga acggatacag
     2401 cttttacaat tgttgttcgt gataccattt ctgattacct cgatttaact agtattcaac
     2461 ctggcgcttc aagtcattct tatacatgga gaagctatgg tgacaatgtg caagctgttg
     2521 aatttacctt taataatatt caattgccgg atagctttgt aaatgaagtt gcttctcacg
     2581 gttttatcaa atatcgaatc aatcaaaagc gctcaaatcc attgggaagt ataattcaaa
     2641 atacagcggc tatttatttc gattttaatg agcccgtgtt taccaataca actttccata
     2701 caatagggga ggacttcctc gaaagtattg tcattacagc aatttctgaa aatgctacaa
     2761 atgagaatcg agtgaaaatc tatcctaatc cgttcacatc cttaactaca ttcgaatttc
     2821 ccaacagtgc accttcacaa ttagtaataa gtgatgcatt aggaagaacg gttaaatcgt
     2881 tgaatactaa ttcggataaa atccaattat caagcgaagg cttggcgaat ggactttatt
     2941 tctatcgtgt ttctagcaat ggaaaacaga ttgatgcagg taaattgatc attcaataaa
     3001 gaagctgaac aatcatttcg ataaaattta aggctgtcaa tcgacagcct ttttttatgg
     3061 tgtaatgaaa tggaaatctt gattcaacat acatgcaaaa caaaaaaggc tgcccaattg
     3121 ggcagccctt tcaatagtat taagcagagt ttagtttgct gctgcttcag gctttggcaa
     3181 taaaacctta cggcttaatt tcaatttacc tgttttctta tctacttcaa tcagttttac
     3241 atcaacaata tcgccttctt taagaatacc atccaggttt tccaagcgac gatgttctac
     3301 ttcagaaata tgaagtaatc cgtctttacc aggcataatt tctacgaatg caccaaatgg
     3361 cataatcgtt ttcactttcc ctgaatacac ttcaccaact tctggtgtgg cagtaattgc
     3421 acggatacga gccaaggctt tatcgatgtt cgctttgtta gcagaagaaa tttgaacaac
     3481 accttgctcg tttacttctt caatcacgat aaccgtttga gtatccttct gcatttcctg
     3541 aatgattttt ccaccaggtc cgattacggc accaatgaat tctttaggaa tacggatttc
     3601 aacaatacga ggagcatgtg gtttgtaatc ttcacgcggc gccgttagaa ctttgttcat
     3661 ttcacccaaa atgtgaagac gaccttcttt cgcttgattc aatgcattga gcatgatttc
     3721 gtaactcaaa ccttgaactt taatatccat ctgacaagca gtgataccat cgcgtgttcc
     3781 ggttactttg aagtccatat ctcctaaatg atcttcatca cctaagatat cgctcaatac
     3841 cgcgtatttg ctaccatcat cggtaatcaa tcccatggca atacctgaaa ctggtttcgc
     3901 gattttcact ccggcatcca tcaatgctaa tgttccagca caaacagttg ccatagaaga
     3961 ggaaccattc gattcaagaa tatcagatac aacacgaatc gtgtatggat tatctgttcc
     4021 ttgaggaatc attggtttca atgcacgtaa tgcaagatta ccatgtccaa cttcacgacg
     4081 actagtacca cgatttggac gtgattcacc agtagagaaa gaagggaagt tataatgtaa
     4141 aaggaagttt gattttcctt ctaacacagc accatcaatg gtttgttcat ccattttggt
     4201 acctaaagta acagttgtta acgattgtgt ttctccacga gtgaagatag cagaaccgtg
     4261 agcaaatgga aggtaatcta cttcgcccca aattggacga atggtagttg tattacggcc
     4321 atccaaacgt tttccttcat taaggataac ttgacgaacc gcttttttct caacatcatg
     4381 gaagtaaaca ccaatgaatg gtttagctac tgctaattct tcttcagaaa gtgaagctac
     4441 aaactcttct ttaactgctt tgaaagcagc tgaacgagcg tgtttgtctg catttcctga
     4501 acgagcaatc tctacacatt tgtcatagca aaatgtatga acagctgcac gaacttcctc
     4561 attatgtgtt tcgtgagaat atacacgttt aggttgagaa gcaggtacta aagcagcgag
     4621 ttcagtaatt gcattgattt gaactttgat agcttcgtgt gcaagcttaa ttgcttccaa
     4681 catatcatgt tcctgaactt ctttcatttc accctcaacc atcacaatac tatcggttga
     4741 cgcagcaacg ataatatcta aatcagcatt tttcagctca tcgattgttg ggttaatgat
     4801 caatttacct tcaacacgag caacacgcac ttccgaaaca ggtccgttaa atggaatgtt
     4861 actcattgct aaagctgccg aagctgccaa acaagctaat gaatcaggta attgagtacg
     4921 atctgaagaa atcagattga tggtgatagt tgtatcagaa tggtaatctt ctggaaaaag
     4981 cggacgtaaa gcccggtcta ccaaacgaca aatcaaaatt tcgttttccg atggacgagc
     5041 ttctcgctta aagaaaccac caggaaaacg acctgttgaa ccatattttt cttggtactc
     5101 aacagttaat ggaagaaaat caacgccttc tttagcgtct tcattgctga caacagctgc
     5161 caacaacatg gtgccaccca tggtaatcac gacagagccg tgggcttgtt tggcaagttt
     5221 acctgtctca atggtaattt ccttaccatc gggcatagca aatgtcttgc gcactccaat
     5281 attcatatag aggctattag gtgtagtgta aaaaaggaaa aaggcaatcc gtgaattgcc
     5341 tttttcagaa acttatttcc ggatattcaa ttctttaaga atcgcccggt atttttcgat
     5401 gtcgcgtgct ttaaggtaat ccagcatact gcggcgttta cctacaagac gaatgagtga
     5461 acgttgtgtt ccaaagtctt tcttgttgac tttcaaatgt tcagtaagat cagaaatgcg
     5521 ttgagtaaac atcgcaatct gcgcttctgt agagcctgtg ttggtctctg atccaccaaa
     5581 tttggtgaag atttcttttt ttaattctga cgtcaatgcc atttttatgt gtcttctttt
     5641 acaggagcgc aaagctaagt aaaaaatctg tgcctgaata aactactcct aaaaaattaa
     5701 caatctctat gtgaataagt ctgaatttga cttttcacgg gcgttttcat gtttaggaga
     5761 atgcgttttt aaccacagga gtacagtttt gcttcataag aaggcgtaca atctgagcta
     5821 ttttaaactt taaatcgcgt ctttcaatga tcaaatctag aaaaccgtgc tctaaaacaa
     5881 actcagctgt ttgaaatcct tccggtaaat ccttaccaat ggtttctttt actacacgtg
     5941 gaccagcaaa tccaatcaaa gagcccggtt cagcaatgtt caaatctcct aacatggcat
     6001 acgaagcagt aactccacca gttgttggat cggtgaggag agagatgtag gggagacctg
     6061 cgtcgcttaa ttgggtaagc ttggccgatg tttttgccat ctgcatcaat gaatatgcgg
     6121 cttccatcat acgtgcacct cccgatttac tgataatcat aaagggaagc ttgtgcttaa
     6181 tgctataatc aatcgatcgg gaaatctttt ccccaacgac agagcccata gaaccaccaa
     6241 taaattcaaa attcatacag gcgataacca tcttaacacc atcgaccgga ccaacaccaa
     6301 ccgaaatagc atctttataa cccgatttag ccatacttgc caccaagcga tctttgtatt
     6361 tcttggtatc ttcaaaaacc aaaggatcag ctgaacgtat gttattgaga atctcttcgt
     6421 atttattgtc atcaaacaaa accgaaaaat actccctaga attcacgcgt tcgtggaaac
     6481 cacaattcga gcaaacccaa agattggctt catgatcaat cgatgcaaag atcttcttgc
     6541 aggatgggca tttgtaccat aatccttctg gagtttcttt tttctccagt gtggaagtgg
     6601 tgatgttttt gttttttcga ctaaaccagc tcattcacaa taagttgtaa ttgattactt
     6661 aaacgaattt ttcaatccat caatcaattg agggctacaa acctaatgga tttcgggaca
     6721 acagcaaatg cttttatata ggatgatgat ctatctaatt gttaaacaga caatttgtta
     6781 atcaacagaa ttcttgagca tgaaaatcaa aacgaatttg aatcaatatt ctatacggta
     6841 aaatcctgac gaaatctgcg agttcatttt tttcatacgt aacgatacga ttctcagcaa
     6901 gtagctaatt cccaaattat gcatatcgac atcaatcaag gtaatttccc aaaagcccat
     6961 aaacaatgga tttctcgaat atttatttgt tatataatta atattatgtt aaatagaaat
     7021 atacaagaaa agaggatttt taggtcctct atcttggttg agattattgt cctaatcgtt
     7081 tcttcatttc attgtactta tcagtcatgt ttaacagact gtaaatttgt ttcaatgttt
     7141 tgagaagcgg ttgtaaatcc ggactgtctt tcggtgctaa ctctaacgct ttctccaagt
     7201 ttggtaatgc ttggttaaat tcagcttctg ctagtttctt gcctgcttcg tatttcttgt
     7261 tatccttctc gttgttggca atatcctgca aacgtttcgc ttcgttgttg tacaaagctc
     7321 ctaagttgaa ataaccttcc cagaaatcag gtttaatctc gattgctttc ttgtatgctg
     7381 caatagcttg ctcacgttta cccaaatctt cgaaaatggt tccagatgcg taatgcaaca
     7441 aatgattagt tggatccttt tcaatggcaa tagctagcaa tttctccgct tctgctgatt
     7501 ttccgccttg caagtagttg ttcaac
//
LOCUS       DAOOQW010000110         7407 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_12329_length_7407_cov_4.846300, whole genome shotgun sequence.
ACCESSION   DAOOQW010000110 DAOOQW010000000
VERSION     DAOOQW010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 7407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7407
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12329_length_7407_cov_4.846300"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1742)
                     /locus_tag="PK281_07270"
     CDS             complement(<1..1742)
                     /locus_tag="PK281_07270"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014031379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOY49184.1"
                     /translation="MTTFSELGLRPELLEALTQMGFTTPTPIQEQTIPLLTENITDLV
                     ALAQTGTGKTAAFGLPLLNILDEERRTPQALILCPTRELCMQITKDLEAFSTKMPQVK
                     IVAVYGGAAISTQISQIKRGVQIVVATPGRLKDLIDRKAIALGEVETLVLDEADEMLN
                     MGFRDDIDYILATTPEEKQTWLFSATMPSEVASISKRYMVSPVEISVGKKNQGATNIE
                     HLYYMVSNHNRYNALKRIADFHPDIYGIIFCRTKMETQEIAEKLIKDGYNADSLHGDL
                     SQAQRDSVMRKFRNKNLQMLVATDVAARGIDVDSITHVIHYNLPDDSESYTHRSGRTA
                     RAGRSGISLAIITPRDLNKIRQVEHRIGTQFAHKQVPTGIEVCEQQLIYLAKKAHDVA
                     VNPDIEQYLSSILETFEDMSKEEVLKSFLSLEFNRFLEYYKNASDLNEGRGSRSEGGR
                     PVGIPMTRYFVGVGELDGIDKSGMVRLLCDTTGLPNDKIGKIELRRSFSFFEVPPQFE
                     HIVESKFEDVQVNGRTIRIEKTTGGGDGGERKRGGGYGGGSGGGKRWDSDRPSAGKSR
                     PYREKESGGGYRSSG"
     gene            complement(1979..2367)
                     /gene="rnpB"
                     /locus_tag="PK281_07275"
     ncRNA           complement(1979..2367)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PK281_07275"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(2589..2999)
                     /locus_tag="PK281_07280"
     CDS             complement(2589..2999)
                     /locus_tag="PK281_07280"
                     /inference="COORDINATES: protein motif:HMM:NF039977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6069 family protein"
                     /protein_id="HOY49185.1"
                     /translation="MNSKLTFVQSIKAGAMASGVAAVINAILFFVFHAGGILVDTIYI
                     QPGQALTVVPILLSSIMPTLIASIVFFLLEKYTKNGLNIFRIISLVLMVLSFANPFMI
                     PDVTLGYAIVLNVMHVVVVACLLFFIGKAVKENK"
     gene            complement(3066..3518)
                     /locus_tag="PK281_07285"
     CDS             complement(3066..3518)
                     /locus_tag="PK281_07285"
                     /inference="COORDINATES: protein motif:HMM:NF013231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOY49186.1"
                     /translation="MKLEEALKQTKPFKNEIHKLILNIHYTSSCLNAVLADLLKPYDL
                     SPHQFNVLRILKGNHPKTYCNQEISSRMIDKNSNATRIVDKLITKNLVSRITCDVDRR
                     QVQIGITEKGLALLDELDKAINLDTLILGTITEEEAQLVNPILDRLRT"
     gene            complement(3606..4580)
                     /locus_tag="PK281_07290"
     CDS             complement(3606..4580)
                     /locus_tag="PK281_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HOY49187.1"
                     /translation="MSDIRIGSKVRLLNEVGEGVVTGFNKQGLALVMMSDGFEIPYYP
                     KQLVSIGGMESSGVATFSGSSVASVNATSLKEAFYMAFVLEGILKDQPKISVRLVNQM
                     RESMLIALYSETERTYTLVASGEIQANSSKAVLTLLLPELLQLDRFFIQIQPLTGNTK
                     ILPASWGGYIKHQTPAMIEPSSWPQQLVFTERALMIEVYPDKGGNQSNIPNKDAIPRK
                     TTTLSQDWLLKQGRDGNYEVDLHIEELLEDTAGMDNSEIIRHQLRHFEKCLDEARRRP
                     VKRFVVIHGVGKGRLRDEIRKLLTADKIEHYDAPLSKYGYGATEIIVK"
     gene            complement(4611..5171)
                     /locus_tag="PK281_07295"
     CDS             complement(4611..5171)
                     /locus_tag="PK281_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49188.1"
                     /translation="MKQQLFESDRISIKESPDELEIIISGNVPKNQFVMLSAWLIAWT
                     FAGGYVISQLFTNLPDNTRIFMFVWLVFWFYFEYKVGSAWLWRKYGREVLRMRADKTE
                     LRFEVAYGGRASEFNTSEIRNLSNMEQQKGVFVKNYYSSFWVVGGETIGFYYQGKLYL
                     LGRQISQKDTDLLMQKIGNRLRKWAP"
     gene            5340..7004
                     /locus_tag="PK281_07300"
     CDS             5340..7004
                     /locus_tag="PK281_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49189.1"
                     /translation="MKKTSFLAVILLICFKAIAQMPTLTVGGSVASLDKVPLNILGET
                     QGKIVVLSWVPSMSVGASMFNSSFRLKDYRIALHDSKTLDETDLLKFPELQVKGIEKT
                     KKTYVEEMILRGDTIQIFATVEDGKTDEQVIVIWNLNALTLKPYEQYAQEIGRFYDRK
                     RSDYKAVTISEQKKYNRIIVAFFNYNDKTENTSLDIKLFDNDLNPISTSHAKIGKSKF
                     GSGVQNIDVDREGNIYVIYAVAEKYNPGWEDNRWVNVLIVSKDGGEPDIVEIKLNNGD
                     AINGEVVVSAKGEVNIIGQYAKLIDAEDKPKELFAGTYLVRVDPETKEITKQEEIELT
                     DNQKISLTTRTLKPIPAERSRWREMINMRVSNIYTDPSGTMTLVSYPHYAVGTSSSAY
                     YFCNSLIVTQFAPEGTINWQTLVPRAAFINGLNYALDPFIFYIDGQVRVLFNDHGKNK
                     EYLAEGPKKSTSGKKVKGSDDVDAPIELNAWNVGNTALRFTTINSDGTMNVEWLEQVG
                     KDEDTKVPAIEASVKYKINDHEYLTAVYTKYGMLGFQKNAISRITF"
     gene            complement(7016..>7407)
                     /locus_tag="PK281_07305"
     CDS             complement(7016..>7407)
                     /locus_tag="PK281_07305"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOY49190.1"
                     /translation="KTFFHGHSFTANPIACRAALASLELFDNPACLENIQRIVEAHAQ
                     FADEIKGNQSIEEIRILGTILAIELKTVDERGYMNPISEQITKHFLNNGIYLRPLGNV
                     IYCTPPYIINEKELQLIYDAIRQFLNS"
BASE COUNT         2260 a         1545 c         1355 g         2247 t
ORIGIN      
        1 cctgatgagc gatatccgcc accgctttct ttctcacggt atggacgaga ttttcctgca
       61 cttggacgat ccgaatccca gcgttttccg ccaccacttc cgccgccata tccaccacca
      121 cgttttcttt cgccaccatc accgccaccc gtagtttttt caatacgaat cgtacggcca
      181 ttcacctgta catcttcgaa tttactttct acaatgtgtt caaattgagg tggaacttcg
      241 aagaacgaaa agcttcgacg caattcaatc ttaccaattt tatcattcgg taaaccagtc
      301 gtatcgcata acaaacgaac cattcctgat ttatcaattc catcgagttc tcccacgcca
      361 acaaaatagc gtgtcattgg aataccaact ggacgaccac cttctgaacg tgatccgcga
      421 ccttcgttca aatcagaagc attcttgtag tattccaaga aacggttgaa ctcgagcgat
      481 agaaagcttt tcaaaacttc ttctttactc atgtcctcaa atgtttcgag gatagatgat
      541 aagtattgtt cgatatcggg atttactgca acatcatgcg ctttcttcgc taggtaaatc
      601 aactgttgct cacatacttc aataccggtt ggaacttgtt tgtgcgcgaa ttgagttcca
      661 atacgatgtt caacctgacg gattttattc aaatcacgtg gagtaataat cgccaacgaa
      721 attcctgaac gacctgcacg agcagtacgt ccgcttcggt gtgtatagct ttccgaatca
      781 tctggaaggt tatagtgaat aacgtgagta atgctatcca catcaattcc gcgagcagcc
      841 acatcggtag ccactaacat ttgaagattc ttgttacgga atttacgcat aactgaatca
      901 cgctgagctt gactcaaatc gccgtgtagc gaatcggcat tataaccgtc tttaatcagt
      961 ttctcagcaa tttcctgcgt ttccattttg gtacggcaga aaataattcc ataaatatcg
     1021 ggatggaaat cggcaatacg tttcaaagca ttgtaacgat tgtgattgct caccatgtag
     1081 tacaaatgct caatattcgt agcaccttgg tttttctttc caaccgaaat ctctactgga
     1141 gaaaccatgt agcgtttcga aatcgatgca acttccgaag gcatcgtagc agaaaacagc
     1201 caagtttgct tttcttcagg agttgttgct aaaatgtagt cgatgtcatc acgaaagccc
     1261 atgttgagca tttcatccgc ttcatctaaa acgagcgttt caacctcacc taaagcaatt
     1321 gctttacgat caatcaaatc tttcaaacgt cccggtgttg ccaccacgat ttgaacgcca
     1381 cgtttaatct gactaatttg tgtggaaatc gcagctccac catacacagc cacgattttc
     1441 acctgtggca tttttgtaga aaacgcctct aaatccttcg taatctgcat gcagagttcg
     1501 cgggtagggc ataggataag tgcctgcggt gtgcggcgtt cctcatcgag aatgtttagc
     1561 aaaggcaatc cgaaagcagc ggttttcccg gttccggttt gagcaagtgc aacaaggtcg
     1621 gtaatgttct cagtaagcag cggtatcgtt tgctcctgga taggcgttgg agtcgtaaac
     1681 cccatctggg tcagcgcctc cagaagctcc ggacggagcc ccaattctga aaaagttgtc
     1741 attgaatata ttgagcggca aaggtacgct tattcatcaa ccagtcgaca tccttctaaa
     1801 tcgcagcttt gaatcgttca aattgcttgt aaatactagt gttacaaact ttttctttca
     1861 ttcaactcaa gcaacttatg aacataactg aagtgccaat tccgtcgaat ttctttcaca
     1921 tcaacaggat tttttgtccc tttttatata aaatcggatt ttaaccggtg ttctcaaaaa
     1981 ataaaacagt cctataagcc gggttctgtc ctgctctcga aagaacagtc cttgtcattt
     2041 atctgggcct gccgtcacca acaggctcta gcgccctacc cgcaagagcg atccgaggat
     2101 cttagacgag cagtctcatc tcttgcctat ttggactttc aactcctgag gtttgccatc
     2161 acagctgtcg ccaactgcgt cgtgggctct taccccgcgt tttcaccctt acctcatttt
     2221 gcaatggggc ggtattttct ctgtggcact ggctgtcaac ggttagttac ccttccatcg
     2281 ccttcccgtt aggaagcagg atgctctatg ttgcccggac tttcctcgcg atacacgaat
     2341 gtacctcgcg acaaggtgga ctgtttgccg caaagatagc tgatactttc atgcactgga
     2401 ggataaaaag atgttgggca actggtttca aatcttcgtt tcacttcact ttaaaaccat
     2461 cgggctttcg gctcgcggga gctcgcctct atccctgttg cgagtgctaa aacgaaaaat
     2521 tcttcccccg aaactagcat tacactcatc tcgaaaggaa gaatcctata ttatgaaggc
     2581 ttttatgcct atttattttc cttaactgct ttaccaataa agaaaagcaa acacgctacc
     2641 accaccacat gcatcacatt caaaacaatg gcataaccta atgttacatc aggtatcata
     2701 aatgggttgg caaaggaaag aaccattaat accaacgaaa taattcgaaa tatgttcaac
     2761 ccgtttttag tgtatttctc taataagaaa aacacaatgc tagcaataag cgtaggcata
     2821 atgctcgaaa ggagaatagg aaccactgtt aatgcttgtc cgggttgaat gtaaatcgtg
     2881 tcaactaaaa ttcctcctgc atgaaaaacg aaaaagagaa tggcattgat aacagcagcg
     2941 acaccagaag ccatcgctcc ggctttaatt gattgtacaa aggtgagttt tgagttcata
     3001 ggttgggtgt ttggaatcgg ttgtatgtat taaagttgta ggtacaaata aaagggcaaa
     3061 aaaaatcagg ttcgtaagcg atctaaaatt ggattcacca gttgtgcttc ttcttcggta
     3121 atcgttccta aaatcaacgt atcgagattt attgctttat ccagttcatc taataaggct
     3181 aaaccttttt cggttatccc aatttgcact tgccgacgat caacatcaca cgttatgcgg
     3241 gaaacgagat tcttggtaat tagcttatct acaatccgag ttgcattcga attcttatca
     3301 atcattctgc tcgaaatctc ctggttacaa taggtctttg gatgattccc tttcagaatt
     3361 cgtagtacat taaactggtg tggacttaaa tcgtaaggct ttagtaaatc tgccaaaact
     3421 gcgttcaaac agctactggt ataatgaata ttcaaaatca acttatgaat ttcattctta
     3481 aatggcttgg tctgctttaa cgcttcttcc aatttcatag gacaaaaata attaaattaa
     3541 ttgtatgtac aattaaaaag tggatttttg ctcgatatat ttgcaattaa ctgatagcca
     3601 attttttatt tcacaattat ttcagttgct ccatatccat acttcgataa tggtgcatca
     3661 taatgttcaa ttttatcagc tgtgagcaat tttcgaatct catcacgtaa gcgacctttt
     3721 cccacaccgt gaatgactac aaatcgtttt acaggtcgtc gtcgagcttc atccaagcat
     3781 ttttcaaagt gtcgcaattg gtgacgaata atttccgaat tatccatgcc agcagtgtct
     3841 tccaataatt cttcaatatg taaatctacc tcataattcc catcacgccc ttgctttaaa
     3901 agccagtctt gcgaaagggt agtcgtttta cgcggaatgg cgtctttatt tggaatattc
     3961 gattgattgc ctcctttatc aggatacact tcaatcatca aagcccgttc ggtaaaaacc
     4021 aactgttgag gccagctgga aggttcaatc atagcgggag tttggtgctt aatatatcct
     4081 ccccaagatg ctggtaaaat tttcgtgttt ccagttaatg gttgaatttg aataaagaat
     4141 cggtcgagtt gtagcaattc gggtaggagc agagttagaa ccgctttact agaattggcc
     4201 tgaatttcac cacttgcaac caaagtgtac gttctttctg tttcgctata aagtgcaatc
     4261 aacatcgatt ctcgcatctg attgacaagt cgaaccgaaa ttttgggttg atcttttaat
     4321 attccttcaa gcacaaaagc catataaaaa gcttccttga gcgaagtcgc attaaccgat
     4381 gccacactgc ttcctgaaaa agttgcaacc cccgaacttt ccattcctcc aatactcaca
     4441 agttgctttg gataatacgg aatttcaaat ccatcactca tcattactaa agcgagtcct
     4501 tgcttattaa agcccgtcac aacaccttcg cctacctcgt taagtaaacg cactttcgat
     4561 ccaattcgaa tatcgctcat tctataattt gtaaataata ttaaggctta ttaaggcgcc
     4621 catttcctta atcgatttcc tattttctgc attaatagat cggtgtcttt ctgacttatt
     4681 tgtcggccta ataaatacaa ttttccttgg taatagaacc caatcgtttc tccaccaaca
     4741 acccaaaagg aagaatagta attcttcaca aatacccctt tttgttgttc catattgcta
     4801 aggttgcgaa tctcagaggt gttaaattct gaagctcgtc cgccataagc tacttcaaaa
     4861 cgaagttcag ttttatcagc acgcattcgt aaaacttccc gtccatattt tctccaaagc
     4921 catgcggagc caactttata ctcaaaataa aaccagaata ccaaccacac aaacatgaaa
     4981 atacgtgtat tgtcgggtaa attcgtgaat agttgactta taacatagcc tcctgcaaaa
     5041 gtccatgcaa ttaaccaagc actaagcata acaaactggt tcttgggaac attacccgaa
     5101 attatgattt cgagttcatc aggagattcc ttaatgctta tcctgtccga ttcaaataat
     5161 tgctgtttca tttggatgca agtttaatca attgatacac gctctccata aactcaccta
     5221 gtttctaatt ctttttcata tttacgataa catttttagt gataaaatat ttatttcttt
     5281 aaacttttaa tatttagcat aatacttata ttgccaatca aatcttccct taatattata
     5341 tgaaaaaaac gtctttttta gcagtaattc tactgatttg tttcaaagca atcgcacaaa
     5401 tgcctacatt aacagttgga ggtagcgtag cttcattaga taaagttcct cttaatattt
     5461 tgggagagac tcaagggaaa atagtcgttt tatcttgggt accaagtatg tcggtagggg
     5521 catctatgtt taattcctct ttccgactca aagattatcg aatcgcatta catgattcaa
     5581 aaacattgga tgaaaccgat ttactcaagt ttccagaatt acaggttaaa ggaattgaaa
     5641 agacaaagaa aacctatgta gaggaaatga ttttacgagg tgatactatt caaatctttg
     5701 ccactgttga agatggcaaa acagatgagc aggtaattgt tatttggaat ttaaatgcgc
     5761 ttacgttaaa accttatgaa caatatgccc aagaaatcgg tcgtttttat gaccgtaaac
     5821 gtagtgatta caaagccgtt actatttctg agcaaaaaaa atacaatagg attatagtag
     5881 cttttttcaa ttacaacgat aaaacagaaa ataccagttt ggatattaaa ttatttgata
     5941 atgacttaaa tccaatttct acatcacatg cgaaaattgg aaaatctaaa tttggaagcg
     6001 gtgttcaaaa tattgatgtt gatagagaag gaaatatcta tgtaatttat gcagtagcag
     6061 aaaaatataa tccaggatgg gaagacaatc gttgggtgaa tgttttaata gtatcaaaag
     6121 atggtggaga gcccgatatt gttgaaataa aactcaataa tggggatgcc attaacgggg
     6181 aagtagttgt ttcagctaaa ggtgaagtaa atattattgg acaatacgct aagcttattg
     6241 atgctgaaga caagccgaaa gaactttttg caggtactta tttggttaga gttgatccag
     6301 aaacaaaaga aataactaaa caagaagaaa ttgaattgac agataatcaa aaaatatcgc
     6361 taacaacacg tacattaaaa cctattccag cagaacgttc tcgatggaga gaaatgatca
     6421 atatgagagt tagtaatatt tatacagatc catcagggac tatgacatta gtatcctacc
     6481 cacattatgc tgtaggcacg agctctagtg cttattattt ttgcaattcg cttattgtaa
     6541 cgcaatttgc tccagaagga acaattaatt ggcaaacttt agttccacgc gcagcattca
     6601 taaatggatt gaattacgca ttagatccat tcatttttta tatagatgga caggtacgag
     6661 ttctcttcaa tgaccatgga aaaaacaaag aatacttagc agaaggcccc aagaaaagta
     6721 caagtggtaa aaaggtaaaa ggttctgacg atgttgatgc accaattgaa ttaaatgctt
     6781 ggaatgttgg aaatacagca cttcgtttca caactataaa ttctgatgga accatgaatg
     6841 tcgaatggct agagcaagtg ggcaaagacg aggacacaaa agttcctgca attgaagctt
     6901 cggtgaagta taaaattaat gatcatgaat acctaacagc tgtctacact aaatacggta
     6961 tgttaggctt tcagaaaaat gccatttccc gaattacgtt ttaaataatt tccacctaac
     7021 tattaaggaa ctgcctgata gcatcatata tcagttgcag ttccttttcg ttaattatat
     7081 aaggaggtgt gcaataaatt acatttccta gcggacgaag atatattcca ttattcagga
     7141 aatgctttgt tatctgttcc gaaatgggat tcatataacc ccgttcatcc actgttttta
     7201 attcgattgc gagtatcgtt cctaagattc gaatttcttc aatcgattga tttcccttaa
     7261 tctcatctgc aaattgagca tgtgcttcaa ctattcgttg gatattctcc aaacatgctg
     7321 gattatcaaa taattccaag gaagctaagg cagcacgaca agcaattgga ttggcagtaa
     7381 atgaatgacc gtgaaaaaat gttttgt
//
LOCUS       DAOOQW010000111         7318 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_12516_length_7318_cov_4.713066, whole genome shotgun sequence.
ACCESSION   DAOOQW010000111 DAOOQW010000000
VERSION     DAOOQW010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 7318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7318
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12516_length_7318_cov_4.713066"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(116..841)
                     /locus_tag="PK281_07310"
     CDS             complement(116..841)
                     /locus_tag="PK281_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HOY49191.1"
                     /translation="MDINKKGGHEDKIIEAFKEKDNFEIKTTNSWQIFKIMAEFVEGF
                     EKLGKIGPCVSIFGSARSKPEDPYYIMAEDIAYRLTMEGYGIITGGGPGIMEAGNKGA
                     HRGGGKSVGLNIVLPHEQEGNRYIDRDKIINFDYFFVRKVMFMKYAQGFVIMPGGFGT
                     LDEFFEAITLIQTNKIARFPLVLVGKKFWGGLVEWVKEQQMRENPFIAEEDLNLFSLV
                     DTAEEAVQAINDFYNKYMFRPNF"
     gene            complement(831..3683)
                     /gene="uvrA"
                     /locus_tag="PK281_07315"
     CDS             complement(831..3683)
                     /gene="uvrA"
                     /locus_tag="PK281_07315"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOY49192.1"
                     /translation="MEIIDSVGEASIEVYGAREHNLKNIDVSIPRNKLVVITGLSGSG
                     KSSLAFDTIYAEGQRRYIESFSAYARQFLGNMERPEVDKITGLSPVISIEQKTTGKNP
                     RSTVGTITEIYDFLRLLFARAGEAFSYVTGEKMIRYSEQQIQALILQDYHEKRISLLS
                     PVVKGRKGHYRELFEQIRKMGYLKVRVDGQVLDITFGMKLDRYKVHDIEIVIDRIEVT
                     NDVTQRISESLKLAMKHGKGSMMVLDADTNEVRHFSRFLMCPTSGISYDEPAPNLFSF
                     NSPYGACPRCHGLGKVTEVDLNKIIPDPKKSLRNGGIVPLGEYKNNWIFAQIEAIADK
                     YSFNLKDPIEDIDESAINAILYGTEEILKIKNPNYNSSGYAMQFNGIVNFITEQHEES
                     SSENTKSWALEYMNDVECPECNGDRVKKEALWFRIDGKNIGELARQDIAHLEVWFKDI
                     ESRMEERQLAIAQEVVKEIRTRIGFLMDVGLEYLTLHRSAGSLSGGESQRIRLATQIG
                     SKLVGVLYILDEPSIGLHQRDNVRLIRALQELRDSGNSVLVVEHDKDMILAADHVIDI
                     GPGAGVHGGRIVAQGPGSTMMLGDSLTAKYLRNEIEIQVPEVRRKGNGKVLKLVQASG
                     NNLKNVTVEFPLGTLICVSGVSGSGKSTLINETLYPILSQYFYRSNKKPLAYKKIEGL
                     QHIDKVIDIDQSPIGRTPRSNPATYTNVFSDIRNLFASLPEASIRGYKAGRFSFNVKG
                     GRCETCSGGGMQVIEMNFLPDVYVQCPSCQGKRYNRETLEVRYKGKSISDVLNMTIDQ
                     AVEFFANIPSILHKIKTLQDVGLGYISLGQSSTTLSGGEAQRVKLATELSKKDTGSTF
                     YILDEPTTGLHFEDIRILLDVLQKLVDRGNTVLIIEHNMDIIKVADHLIDIGPEGGER
                     GGNVLAFGTPEKVAKHPESYTAEFLKKELNSITHGH"
     gene            3682..4764
                     /locus_tag="PK281_07320"
     CDS             3682..4764
                     /locus_tag="PK281_07320"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF025341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HOY49193.1"
                     /translation="MEKQTAKLVILFPKTAIVNAHSCFCTFAFRMIKMQGFFRLTILG
                     LLVIFSGSLVAGFDLNSPLVKRKKGRGSDFPDPIPQGESAKISIPIKRAGNLILMEVQ
                     IDSIVGNLILDTGAPHLVLNKTYFRNYPLYGNVMASGITGGGGAGNQTRVSRLVVAPG
                     LYYSDVDADIVPLGHLESRKGVKIIGLFGLNLLKRLEIEFHLDEGELIIYRLSRSGAR
                     TQVETAEEKLGKAKTIRIFLENGLLLVPVTINEKRLRFYIDTGAETNILHSGSSKKVF
                     EKVQLSRRTMLSGTGKEQAEVLSGELSEMFVDGHSYINMDVTIANLNSLRSVYDSSID
                     GMLGYSFLSRRKLAINFMKGELYLWD"
     gene            4755..6233
                     /locus_tag="PK281_07325"
     CDS             4755..6233
                     /locus_tag="PK281_07325"
                     /inference="COORDINATES: protein motif:HMM:NF027875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49194.1"
                     /translation="MGLNHLFNLWSFKKPTSWIFLFLLCLQVIATAQPAKNPTYTLSE
                     GEMLISIPNGCDSSLVDSLIIHYNLQDIGIKEGYQTGNWTKARQLGWVVKEVKKSMIL
                     KKSLSELSGLDELKMFLMLTSQQGEINPGYPVMIGPLGVNNFKKSELQPKFINDSLCV
                     FQLSGFTQAEHVFISGSFNNWSTINLPLSKTNTGWDIELKIKPGKHLYKFIVDGKWME
                     DPNNSQWESDGFNGKNSVYYHSNVLFKIPGYLNAKKVILAGNFNDWNERELRMKLNGD
                     AWELPVYLEDGTYGYKFIVDGTWYHDTANPNSVSDGSGGLNSIITLGKPHLFRLPGYE
                     NAGQVRLAGDFNAWNPDELNLSKVDGGWELPYVLKPGNYTYKYIVDNSWILDPSNPQT
                     TSDNNGIVNSFVTIGANFRFVLKDHLDKKEVKLAGDFTNWEASALTMKREELGWSIQV
                     YLSPGKHLYKYIIDGTWVTDPQNPLWEENEVGTGNSVIWQIP"
     gene            complement(6230..7210)
                     /locus_tag="PK281_07330"
     CDS             complement(6230..7210)
                     /locus_tag="PK281_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008636235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HOY49195.1"
                     /translation="MSLSFLLGTLGIVRLFNFSARVQTNDDLYQKYFTDSYKVFSVNI
                     PNDLNFAGEPVPLQDFEVRERIDREFLVNTYWQSQTLLLAKRANRWFPVITPILKRNG
                     IPDDFKYLAVVESGFMHGVSSKGAAGYWQFIPSTAQAYGLTINEEIDERYHVEKSTEA
                     ACKLFNEAHDALGNWTLAAASFNIGLNGIQRQLIRQKANNYYDLLLNEETARYVFRVL
                     AIKEILSNPRRYGFIYRKKDLYPPIPSQTIQVDSSITDLALFAEQQGINYKLLKYFNP
                     WLRSETLSNPDHIKYKIKMPHPSVKNYDALMKMAEVELATPAVPVSDTIN"
BASE COUNT         2148 a         1405 c         1448 g         2317 t
ORIGIN      
        1 ccgattttta ttgaaaattc tttttgagac tcaaattccc ttcctctgaa atatgcgagc
       61 acaaaaaaag ctatctgatt tcttcagata gctttccatt aaagttaaat ccagattaga
      121 agtttggacg gaacatgtat ttgttataga agtcattaat tgcttgaacg gcttcttcgg
      181 ctgtatcaac caaactgaac aagttcaaat cttcttcagc aataaatgga ttttcacgca
      241 tttgttgctc tttcacccat tctactaatc cgccccagaa ttttttccct actaaaacaa
      301 gcggaaaacg ggcaattttg ttggtttgaa ttaaggtaat agcttcgaag aattcatcta
      361 acgttccgaa tccgccaggc ataatcacaa atccttgagc gtatttcata aacatcacct
      421 tccttacaaa gaaataatcg aagttgatga tcttatcccg atcgatgtag cgatttcctt
      481 cttgttcatg aggtagaaca atgtttaatc ctaccgactt tccacctccg cggtgagcac
      541 ctttatttcc ggcttccata atacccggac cacctccagt gataatacca tatccttcca
      601 tcgttaaacg gtaggcaata tcttctgcca tgatgtaata tggatcttct ggctttgaac
      661 gagctgatcc aaaaatcgaa acacaaggtc caatttttcc aagtttttca aagccttcaa
      721 cgaattcagc catgatctta aagatctgcc aagagttcgt tgtcttaatt tcaaaattgt
      781 ctttctcttt gaaggcttcg ataattttat cctcgtgccc tccttttttg ttaatgtcca
      841 tgcgtgatac tgttaagttc tttttttaaa aattctgctg tgtaactttc tggatgcttg
      901 gcaacttttt cgggtgttcc aaaggccaaa acattaccac ctcgttcgcc tccttctggt
      961 cctatatcga tcaaatgatc agctactttg atgatgtcca tattgtgctc aataattaaa
     1021 accgtattac ctcggtcaac taatttttga agcacatcga gcaatatacg tatatcctcg
     1081 aaatgcaaac cggttgtagg ctcatccaag atataaaacg tacttccagt gtctttcttc
     1141 gataactcag tggctaattt cacccgctgc gcttctccac cacttaaagt tgtagaagat
     1201 tgtcccaaag aaatatatcc taatccaaca tcctgtaatg ttttgatttt atgtaggatt
     1261 gatggaatat tcgcaaaaaa ctcaactgct tgatcaatcg tcatgttgag tacatcactg
     1321 atagatttgc ctttgtagcg aacttccaat gtttcccgat tgtaacgttt gccctgacaa
     1381 ctcggacatt gaacataaac atctggcagg aaattcatct caatcacttg cattcctcct
     1441 ccactgcagg tttcacaacg tccacctttc acattaaaag aaaaacgtcc ggctttatat
     1501 ccacgaatac tcgcttcagg aagtgatgca aacagatttc ggatatctga aaatacgttt
     1561 gtatatgtag ctggattcga acgtggtgta cgtccaatgg gactttgatc aatatcaatt
     1621 actttatcga tgtgctgaag tccttcgatt ttcttgtatg ctagcggctt tttatttgag
     1681 cggtagaagt attgactaag aatagggtag agggtttcgt ttatcaaagt tgacttacca
     1741 cttcccgaaa cacccgaaac gcaaattaag gttccaagcg gaaattcgac agttacattt
     1801 ttgaggttat ttccacttgc ctgaaccaat ttcaagactt ttccattgcc ttttcgacga
     1861 acttctggaa cctgaatttc gatttcgttt cttaggtatt tagctgtgag cgaatcgccc
     1921 aacatcattg ttgaacctgg accttgcgct acaattctgc caccatgaac acctgcaccc
     1981 ggaccaatat caatcacatg gtcagcagct agaatcatgt ccttatcgtg ttctacaacc
     2041 aagaccgaat tacccgaatc tcggagttct tgtaaagcac gaatcaaacg aacattatcg
     2101 cgttggtgaa gtccaatact tggctcatcg aggatataca aaacgcctac gagcttagag
     2161 ccaatttgcg ttgctaagcg aatacgttga gattctccac ccgataaact accagcactg
     2221 cgatgaagtg ttaaatactc caagccaaca tccatcaaaa atccaatacg tgttcggatt
     2281 tccttcacga cttcctgagc aatagctaat tgtcgctctt ccattcggct ttcgatatcc
     2341 ttgaaccaaa cttctaaatg agcaatatct tgtcgagcaa gttctccaat gttcttccca
     2401 tcaatccgaa accaaagcgc ttctttcttc acccgatcac cattacattc cggacattct
     2461 acatcgttca tgtattcgag tgcccaactt ttagtgtttt cggaagagct ttcctcgtgc
     2521 tgttcggtga tgaaattcac gataccattg aattgcatcg catatccgct agaattatag
     2581 ttcggatttt tgattttcag aatctcctct gtaccatata aaatggcgtt aatggcagat
     2641 tcatctatat cttcaatcgg atctttcaga ttaaaactat acttatcagc aatggcttct
     2701 atttgagcaa aaatccagtt gtttttatat tctcccaaag gaacaattcc cccatttctc
     2761 aagcttttct ttggatcagg gataatctta ttgaggtcga cttcagttac ttttcctaaa
     2821 ccatgacaac gtggacaagc accatacgga gagttgaaag agaagaggtt aggagcaggt
     2881 tcatcatatg aaattcccga agtaggacac atgaggaatc gactaaagtg gcgaacttca
     2941 ttggtatcgg catcgagcac catcatgcta ccttttccat gtttcatcgc tagtttaagc
     3001 gattcggaaa tgcgttgagt tacatcattc gttacttcaa tccgatcgat aacaatttca
     3061 atatcatgca ctttgtaccg atccaacttc atcccaaagg tgatatcgag cacttgtcca
     3121 tccacccgaa ctttgagata gcccatttta cggatttgct cgaataattc acggtaatgt
     3181 ccttttctac cttttacaac gggtgaaaga aggcttattc gcttctcgtg ataatcctgt
     3241 aaaatcagcg cttgaatttg ttgttccgaa taccgaatca tcttttctcc tgtcacatag
     3301 gaaaaagctt cgccagcacg ggcaaacaag agacgaagga agtcgtagat ttcggtaatt
     3361 gtaccaacgg ttgatcttgg attcttaccg gtagtttttt gctcaataga aataaccgga
     3421 cttaaaccag tgattttatc cacttctgga cgttccatat ttccaaggaa ctgtctagca
     3481 taagccgaaa agctttcaat gtatctacgc tgaccttcag cataaatggt atcaaatgct
     3541 aaggatgatt ttccacttcc gctcaaaccg gtaattacaa ccaatttgtt gcgagggata
     3601 ctcacatcta tatttttgag attatgttcg cgggcaccgt aaacttcaat ggaagcctca
     3661 cctacggaat cgattatttc catggaaaaa caaacggcga aattagtcat tttgttcccg
     3721 aaaacagcta tagttaatgc tcattcctgc ttttgtacat ttgcttttcg gatgattaaa
     3781 atgcaaggct tttttcgact taccattctt gggctactcg tcatcttttc gggtagtttg
     3841 gtggctggat tcgatttgaa ttctcctttg gtaaagcgta aaaaagggag aggttctgat
     3901 tttcctgatc cgattccaca aggagaatca gctaaaatta gcatacccat taagcgtgca
     3961 ggcaatttaa ttttgatgga agttcaaatc gattctattg ttgggaatct gattttagat
     4021 acgggtgctc cgcatttagt attgaacaaa acgtacttcc ggaattatcc cctttatgga
     4081 aatgtgatgg cttctggaat tacaggcgga ggcggtgctg gaaatcaaac ccgagtatct
     4141 cgtttggttg tcgcgccagg tttatattat tcggatgtag atgctgatat tgttccactt
     4201 ggacatttag agtcgcgcaa aggtgttaag attattggat tgtttgggtt gaatttgttg
     4261 aagcgtttag aaattgaatt tcacttagat gagggcgagt tgattattta tcggttaagt
     4321 cgttcgggag ctcgaacaca ggtcgaaaca gccgaagaaa aattggggaa agctaagaca
     4381 atccgaattt ttctagagaa tggattactg ttggttccgg taacgataaa tgaaaaacga
     4441 ttacggtttt atatagatac tggcgctgaa acgaatattt tgcattcggg ctcatctaaa
     4501 aaagtatttg agaaagtcca actttcccgt cgtacaatgc ttagtggaac gggaaaagaa
     4561 caagctgaag ttttaagtgg tgaattatca gaaatgttcg tcgatggaca ttcgtacata
     4621 aatatggatg taaccattgc taatcttaat tcacttcgat cggtttacga ttcaagtatt
     4681 gatggaatgt tgggttacag ttttctttcg aggaggaaat tggccattaa ctttatgaaa
     4741 ggagagcttt atttatggga ttaaatcacc tgtttaattt atggtcattt aaaaagccaa
     4801 ctagctggat ctttttgttc ctgctgtgtt tgcaagtgat tgcaacggca caaccggcta
     4861 aaaatcctac ttatactttg tcggaaggtg aaatgcttat ttcgattccg aatggatgtg
     4921 attcttcctt agtagatagt ttgataatac attataattt gcaggatatc ggtattaaag
     4981 aaggatatca aaccggaaat tggacgaagg ctcgacaact tggatgggtg gtgaaagaag
     5041 tgaagaagag catgattctg aagaaatctt tatctgaatt aagtggatta gatgaattga
     5101 aaatgttctt gatgctaact tctcagcaag gagaaataaa tccaggttac cctgtgatga
     5161 taggtccttt gggtgttaat aatttcaaaa agtcagaact tcaacccaag tttataaatg
     5221 atagtttgtg tgtgtttcaa ttgtcaggat ttacacaagc cgaacacgtt tttatttcgg
     5281 gttcttttaa taattggagt acgattaatt tgccattaag taaaacaaat accggctggg
     5341 atattgagct gaaaatcaaa ccgggaaagc atttgtacaa atttatagtg gatggaaaat
     5401 ggatggaaga tcccaataat agtcagtggg aaagtgatgg atttaacggg aagaattcgg
     5461 tttattatca ctccaatgtg ctatttaaaa tacctggtta tttaaatgct aaaaaggtca
     5521 ttttggctgg taattttaat gattggaatg agcgagaact tcgaatgaaa ttaaatggag
     5581 atgcttggga attgcctgtt tatttagaag atggaacata tggttataaa ttcattgtgg
     5641 atggaacttg gtatcatgat actgcaaatc caaactcggt atcggatggt tcggggggat
     5701 taaactccat cataacactt ggaaaacctc atttatttag gttgcctgga tatgaaaatg
     5761 caggacaagt tcgattagct ggcgatttca atgcttggaa ccccgatgaa ttgaatttat
     5821 cgaaagtaga tggaggttgg gaattaccat atgtacttaa acctggtaac tatacgtata
     5881 aatatatcgt ggataatagt tggattttag atccatcaaa tccccaaaca actagtgata
     5941 acaatggaat tgtgaattct ttcgtgacaa ttggtgctaa ttttcgtttt gttttgaaag
     6001 atcatttaga taaaaaagaa gtcaagttag ccggtgattt tacgaattgg gaagcatccg
     6061 ctttaaccat gaaacgtgag gaattgggtt ggtctattca agtgtatttg agtcccggta
     6121 agcatttgta taaatatatt attgatggaa catgggtaac tgatccacaa aatccattat
     6181 gggaagaaaa tgaagtggga acaggaaatt cagttatctg gcaaattcct taattgattg
     6241 tatcgcttac tggaactgca ggagtagcca attcaacttc ggccattttc atcaatgcat
     6301 cataattctt aacacttgga tgaggcattt taatcttgta cttgatgtga tcaggattag
     6361 ataatgtttc tgacctcagc catggattaa agtatttaag gagtttataa ttaatgcctt
     6421 gttgttctgc aaatagggct aaatcggtaa tactactatc tacttgaatg gtttgactcg
     6481 gaataggagg gtataaatct tttttccgat agataaatcc atatctccga gggttactta
     6541 ggatttcttt aatcgctagc acacgaaaaa catatcgagc agtttcttca ttaagaagca
     6601 aatcgtagta attatttgct ttctggcgga tgagttgtcg ttgaattccg ttcaagccta
     6661 tattaaaaga tgcagcagct aaagtccagt ttcctagagc atcgtgggct tcattaaata
     6721 gtttgcaagc tgcttcggtt gatttttcaa cgtgataacg ttcgtcaatt tcttcattaa
     6781 tggttaagcc ataggcttgt gcagtgctag gaataaactg ccaatagcct gcagcacctt
     6841 tactagatac accatgcatg aagccacttt cgacaacggc taaatacttg aaatcgtcag
     6901 gtataccgtt acgtttaagt ataggtgtaa ttacaggaaa ccagcgattg gcgcgtttgg
     6961 caagaagtaa ggtttgcgat tgccagtaag tgttaactaa aaattctcgg tcgatacgtt
     7021 cacgaacttc gaaatcctga agtggaacgg gctcaccagc aaagtttaaa tcgttgggaa
     7081 tattgaccga gaaaactttg tagctatcgg tgaaatattt ctggtaaaga tcgtcgttgg
     7141 tttgaactct tgcagaaaag ttgaacagtc taactatgcc caaagtcccc aataaaaaag
     7201 atagcgacac ggctattttc caccatgttt ggggtattcg gctaaagatc tgtttaaacc
     7261 tcacgtttta cttttaggtt tgctcaataa ggttgttgaa tagagagcgc caccgatt
//
LOCUS       DAOOQW010000112         7174 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_12827_length_7174_cov_4.608372, whole genome shotgun sequence.
ACCESSION   DAOOQW010000112 DAOOQW010000000
VERSION     DAOOQW010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 7174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7174
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12827_length_7174_cov_4.608372"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2770
                     /locus_tag="PK281_07335"
     CDS             <1..2770
                     /locus_tag="PK281_07335"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOY49196.1"
                     /translation="STDSADGDDATDPANYVSCSYSSAATETCSIPNAIAGEFYILLL
                     TNYSGQATNINFQQGGGSGTTDCSIFCSVDATPAPTACDPATNTYILAGTIDFVNPPA
                     TGTLTITNSSGGYMSFNAPFATSVNYNFTNLSSNGNAEQLTVVFSDDNTCAAIGDYTA
                     PASCSQCPVQASVSGPACEGQSLSLSATNVANGQYSWTGPNGFTSTDQNPVLSNVTPS
                     MTGVYQVTALNPMNNCSSISSVNVFVFPTPATPTITNDGPVCEGTPLNFTCNSYPGGT
                     YNWTGQNSFTSTQQNPTIAAASNAANGNYNCTVTVNGCTSLQATTAATINPYPSSPVP
                     SNNSPLCDGDNLQLTSIAIAGATYEWKNPAGTVISSNQNLTLDAVTSSQSGVYSLRVQ
                     VNGCWSLPATTTVNIYPIPTTPVLVSNAPVCENALLTINGPAPLPIVGTTYAWSGPSL
                     FSASSQNISISSSQLSNAGQYSLVITENGCSSAAGTTNVVVIALPVSNAGADLTVCSL
                     EPASIGAPPVAGYTYSWLPVGGLDFSTIANPTAGISNMSGQPNDFTYIVTTSDQMCST
                     KDTVVITVNPQPIASFVSPNPACFEGNSFDFDADGFYSSGATFNWEFGPWASAPTSTL
                     ESPQDISFNSTGLQIVRLTITDLGCESNLYQAPVMVYKMPVSNFTSDFFVGCDPKVIN
                     FTNLSESDDPIKTYVWNFGNGRNSSSATNPAVLYNDPGVYDVSLVVTSEKLCADTFLI
                     NDMIRIYPSPVADFALSPEVVYITKPEMDFNDLSQGADEVFYVVDDTDYVYQADARYT
                     FPDSGIYDVRQVVTTAFGCRDSVTKQAVVELGYKIYVPTAFTPNNDGYNDRFQVYGED
                     VSEFSMLIYNRWGQLLYSSYDMENGWDGRTLLKDRVCDGGVYVYKIAAVQRNGLKTNY
                     EGTLVLLR"
     gene            complement(2843..4351)
                     /gene="gpmI"
                     /locus_tag="PK281_07340"
     CDS             complement(2843..4351)
                     /gene="gpmI"
                     /locus_tag="PK281_07340"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013070460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HOY49197.1"
                     /translation="MKDKKVALLILDGWGIGNGTHSDAIANSTTPVMSRLLRTNPWSK
                     LRTDGLNVGLPEGQMGNSEVGHLNIGAGRVVFQELVKINQSTETAQIDTNPVLVESLR
                     IAREENKTLHFFGLVSDGCIHSSDKHLHHLIALAEKSGVKRFYVHAFTDGRDTDPKSG
                     IGFLTTLQDKLVGTHGKLASIVGRYYAMDRDKRWERVKIAYDMLIHGIAEQSNNLLQT
                     LSNRYDAGETDEFIKPIKLQGEDCTIHPGDVVISFNYRTDRCREITEVLTQKDFPDFG
                     MKTIPLHYVTMTNYDESFKGIDVIFHKDDLSQTLGEVLSTQGKKQLRIAETEKYPHVT
                     FFFSGGQEKAFPGESRIMIPSPKVATYDLQPEMSAPGIRDAVIADVKTNQPDFICLNF
                     ANADMVGHTGVYPAIVKAIEAVDSCLGDVLSCLHDYEYSVIIIADHGNADFAVNPDGS
                     PNTAHSTNPVPCILIDPDHKEIKDGKLADIAPTILRLMNIPIPEIMNGNVLI"
     gene            complement(4356..5330)
                     /locus_tag="PK281_07345"
     CDS             complement(4356..5330)
                     /locus_tag="PK281_07345"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOY49198.1"
                     /translation="MKKPRFFENTPRSLLIFYVLVLYVVFQSCWWYYLLLDLNSQIFD
                     LRSSLAGLLEYDVGLESSLRQKLQQKQWMVFGEGLVFLLLLLLGFIQTRKSFKRETQV
                     ARQQKNFLLSVTHELKSPIASVKLYLQTLEKRELDRSKQIDLLQKAITETNRLDHLVE
                     NMLVAAQIENQALLIQPEEMDLSGFVSAFINEFEEKNQIKISGKRVSGLMIQADPLSM
                     RSILVNLTENALKYGGSNPEIAINLSIDENHVVLQICDQGIGIAEEERQRVFDKFYRS
                     GNEETRQTKGTGLGLFIVKYLVGLQSGSISVLNNTPKGSTFELRFKSI"
     gene            complement(5368..5889)
                     /locus_tag="PK281_07350"
     CDS             complement(5368..5889)
                     /locus_tag="PK281_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015754242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HOY49199.1"
                     /translation="MRYLLFLLLVLPSFLPAQTEGIHWMSFTDAMAKRDKAPKKIFID
                     MYTDWCGWCKRMDASTFIHPEVVKYMNEHYYAVKFNAEKEGPISIRDSVYKVNPQYGR
                     NGTHEIAVEMLQGKLSYPSFVFLDERFNMLSPLPGYQTPEQLEPVLRFFAEGIYLTTS
                     WADYQKKFLPSWK"
     gene            complement(6002..>7174)
                     /locus_tag="PK281_07355"
     CDS             complement(6002..>7174)
                     /locus_tag="PK281_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006257525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HOY49200.1"
                     /translation="LILAFDPQDLFFVGISFTIVGTGFFKPNISTMVGALYHSNDSRR
                     DAGFSLFYAGINIGALLGGYACVTIAKTYSWNLAFGLAAIVMTISLLTFLFTQKSLGP
                     IGLSPITKTGKKWFEYATYAGSLLVIPLIMVMVAKTEYTDYFMYTIGPCTLIYLFYEM
                     SKYSVEERKKLFAALIFIIFSILFWAFFEQSGGSLNIFATNNLHGELFGISVDPNGIN
                     NSANSFFVIAFAALVGLLWVALSKRGKEPNTVVKFGLGFLFLGLAFFVFYATRFFADS
                     EGKTSLEVFTLAYFIITFGELCLSPIGLSIMTKLSPQPLQGVMMGMWFLASAYGQYVA
                     GLLGAGMTAPGENASALEKLTSYTGGYQQLAIYAVICGVLLIVISPMVKKLMGNVK"
BASE COUNT         2146 a         1459 c         1365 g         2204 t
ORIGIN      
        1 atcaacagac tctgctgatg gcgatgatgc cactgatcct gcaaattatg tttcatgtag
       61 ttactcttca gctgcaacag aaacatgtag tattccaaat gctatagctg gagaatttta
      121 tattttacta ctaacgaatt attccggtca ggcaacgaat ataaacttcc aacaaggagg
      181 tggtagtgga actaccgatt gtagtatatt ctgtagtgtt gacgcaactc ccgcgcctac
      241 tgcttgcgat ccagcaacga atacatacat tttagctgga actattgatt ttgtgaatcc
      301 acctgctaca ggaacactta ctataaccaa ttcttctggt ggttatatga gtttcaatgc
      361 tccatttgct acttcagtaa attacaactt cactaacctt tcttcaaatg ggaatgcaga
      421 gcagttgact gttgtatttt cggatgataa tacatgtgct gcaattggtg attatacagc
      481 tcctgcatct tgttcacaat gtccagttca ggcgagtgtt tcggggcctg catgtgaagg
      541 acaaagtctt tctttatcag caacgaatgt ggctaatgga caatattctt ggactggacc
      601 aaacgggttt acatcaactg atcagaatcc agttttatcg aatgtaactc cttcaatgac
      661 tggcgtttat caagttactg cattgaaccc gatgaacaat tgttctagta tcagttcggt
      721 taacgtattt gttttcccta cacctgctac accaacaatc acgaatgatg gaccagtatg
      781 tgaaggaaca cctctaaatt ttacgtgtaa cagttatcct ggaggaacat acaactggac
      841 aggacagaat tcattcacct ccactcaaca aaatccaaca attgctgcag cgtcgaatgc
      901 agcaaacgga aattacaatt gtactgtaac ggttaatggt tgtactagtc ttcaagctac
      961 tacagcagct actataaatc catatccatc ctctccagtt ccatctaaca atagtccttt
     1021 gtgcgatgga gataacttgc agctaacttc tattgctatt gctggtgcaa cttatgaatg
     1081 gaaaaatcct gctggaacag taatttcatc aaaccagaac ctaactcttg atgctgttac
     1141 ttcctctcag tcgggcgttt atagtttacg tgttcaggtt aatggatgtt ggagtttacc
     1201 agctacaact acagtaaaca tttatcctat tccaactact ccagttcttg tttcaaacgc
     1261 tccagtttgt gaaaatgctc ttcttacgat taatggtcct gctcctcttc caattgtagg
     1321 aactacctat gcttggtctg gtccatcgtt atttagcgct agttcacaaa acatttctat
     1381 atcaagttca cagttatcaa acgctggtca atattcgctt gtgattactg aaaatgggtg
     1441 ttcgtctgct gctggaacta caaatgtggt tgtaattgct ttacctgttt caaatgctgg
     1501 cgccgattta actgtttgtt cccttgagcc cgcttccatt ggagctcctc ctgttgctgg
     1561 ttatacttat tcttggttac ctgttggagg attggatttc tcaaccattg ccaatccaac
     1621 agctggaatt tctaacatgt ctggtcaacc gaacgatttt acctacatcg ttactacttc
     1681 agatcaaatg tgttctacaa aagatactgt agttattact gttaatccac aacctatcgc
     1741 atcgtttgta tctcctaatc cagcttgttt cgaaggaaac tctttcgatt ttgatgccga
     1801 tggattctat tcttcgggag caacgtttaa ttgggagttt ggcccttggg cttctgctcc
     1861 aacttcaact ttagaaagtc ctcaagatat ttccttcaat tctacaggat tgcagattgt
     1921 tcgattaact ataactgatt taggttgcga aagtaatttg taccaagctc cagttatggt
     1981 ttacaagatg cctgtttcga attttacttc cgatttcttc gttggttgtg atcctaaagt
     2041 gatcaatttt accaacttaa gcgaatcaga tgatccgatt aaaacctatg tatggaactt
     2101 cggaaatgga cggaattcaa gttctgccac aaatcctgca gtattataca acgatccggg
     2161 tgtttatgat gtatcattag ttgttacctc agaaaagtta tgtgccgata cgttcttgat
     2221 taatgacatg attcgtatct atccttcacc tgtcgctgat ttcgctttaa gtcctgaagt
     2281 ggtttatata actaaacctg aaatggattt taacgacctc tctcaaggtg ccgatgaagt
     2341 tttctatgtg gttgatgata ctgattatgt ttatcaagct gatgctcgtt atactttccc
     2401 agattcggga atctatgatg ttcgccaagt tgttacaact gcttttggat gtcgtgattc
     2461 tgttacaaaa caagcagttg tagaattggg ttataagata tacgtaccaa ctgcatttac
     2521 accgaataat gatggctata atgatcgatt ccaagtttat ggtgaagatg tgtcggagtt
     2581 tagcatgttg atctataatc gttggggaca actactttat agctcttacg acatggaaaa
     2641 tggatgggat ggtcgaactt tattaaaaga ccgcgtgtgt gatggaggtg tttatgtcta
     2701 taaaattgct gctgttcaac gaaacggttt aaagacaaat tacgaaggaa cattagtcct
     2761 cctccgatag aatttactct caccgaaata aaaagccccg ttaaaaacta atttaacggg
     2821 gctttttttg aattcttaat gcttaaatca acacatttcc attcatgatt tcaggaatag
     2881 gaatgttcat caaacgtaaa atcgtaggag cgatatcggc tagtttgcca tctttaattt
     2941 ccttgtgatc tggatcaatc aatatacaag gtaccggatt agttgaatga gctgtgtttg
     3001 gactaccatc cggattaaca gcaaaatcgg catttccgtg atcagcaatt attataacgg
     3061 aatattcata gtcgtgtaaa caagatagaa catcacccaa gcaagaatct actgcttcaa
     3121 ttgctttcac aatagcagga taaactcctg tgtgcccaac catatcggca ttggcaaagt
     3181 ttaaacaaat gaaatcaggt tgattggttt tcacatctgc aattacagca tcacgaatcc
     3241 caggagcact catctcaggt tgcaaatcgt atgtggcaac tttaggtgaa ggaatcataa
     3301 tacgtgattc tcctgggaat gctttctctt gtccacctga aaagaaaaat gttacgtgag
     3361 gatacttctc tgtttcggca atacgtagct gtttctttcc ttgagtggat aaaacttcac
     3421 ccaaagtttg cgataaatca tctttatgga aaatcacatc aattccctta aatgactcat
     3481 cataatttgt catagtaaca taatgcaagg gaatggtttt cataccaaaa tccgggaagt
     3541 ctttctgagt aaggacttcg gtgatttctc tgcaacgatc agttctataa ttgaaactaa
     3601 tcaccacatc acctggatga atcgtgcaat cttcaccttg caacttgatg ggtttaataa
     3661 actcatcggt ttcacctgca tcgtagcgat tacttaatgt ttggagcagg ttgttggatt
     3721 gttccgcaat accatgaatc aacatatcgt atgcgatttt cacacgctcc caacgtttat
     3781 cacgatccat tgcatagtat cgaccaacga ttgatgctag ttttccatgt gtgccaacca
     3841 gcttatcttg aagcgttgtt aagaatccaa taccactttt aggatcagtg tcacgcccat
     3901 cagtaaaagc atgcacgtaa aaccgtttta ctccgctttt ttctgctaat gctatcagat
     3961 gatgaagatg tttatcggaa gaatgaatac agccgtccga cactaaacca aagaaatgaa
     4021 gtgttttatt ttcctcccga gcaattcgta acgattcaac aagaaccgga ttggtatcga
     4081 tttgagctgt ttcggtcgac tgattaatct ttactaattc ctgaaagaca actcttcctg
     4141 caccaatatt caaatgccca acttccgaat ttcccatttg tccctcaggc aatcctacat
     4201 tcaagccatc ggtgcgtagt tttgaccatg gatttgttcg caataaacga ctcatgacag
     4261 gcgtggtcga attagcgatt gcatcggaat gtgtaccatt tccaattccc caaccatcga
     4321 gaattaggag ggcaactttt ttatccttca tgggtttaga ttgatttaaa tcgaagctca
     4381 aaagtacttc ctttaggtgt attgttcagc accgaaatac taccagattg aagtccgacc
     4441 aagtatttca ctatgaataa tccgagtcct gtacctttag tttgacgtgt ttcttcattc
     4501 cctgaacggt agaatttatc aaaaacacgt tgtcgctctt cttccgctat tccgattcct
     4561 tgatcgcaaa tctgtaaaac cacatggttc tcatcaattg aaaggttaat cgcaatctct
     4621 ggatttgagc caccgtattt caatgcattt tcggtgaggt taactaagat agatctcatg
     4681 gatagcggat cagcttgtat catcaaaccc gaaacacgtt tgcccgaaat cttaatctga
     4741 ttcttttctt cgaattcatt aatgaaagca ctaacgaaac ccgacaaatc catttcctcc
     4801 ggttgaatga gtaaagcttg attttcaatt tgagctgcca ccaacatgtt ttcaaccaaa
     4861 tgatcgagtc ggttggtttc ggtgattgct ttttgaagca aatcgatttg tttacttcta
     4921 tccagctctc ttttctctaa agtttgaaga taaagtttta ccgatgcaat aggtgatttg
     4981 agctcgtgtg tgaccgatag taagaagttt ttttgttgtc gagcaacttg ggtttctcgt
     5041 ttaaacgatt tgcgggtttg gataaaaccc aaaagtaaaa gcagtaagaa aaccaatcct
     5101 tctccaaata ccatccattg tttctgttgg agtttttggc ggagtgaact ttctaaacca
     5161 acgtcgtatt ctagcaagcc agctaaactg gagcgtaaat cgaagatttg actatttaga
     5221 tcaagtaata aatagtacca ccaacacgat tgaaacacca catataacac caacacgtaa
     5281 aatatgagaa gtgatcgtgg agtgttttcg aaaaatcggg gtttcttcat gattgggcct
     5341 aataattagg caaatagcaa tgcgttttta tttccagcta ggaaggaatt tcttttggta
     5401 atcagcccaa gatgttgtta agtaaatccc ttctgcaaaa aagcgtaaaa cgggttctag
     5461 ctgttcagga gtttgatagc ctggcagcgg acttaacatg ttaaagcgtt catccaagaa
     5521 aacaaacgat ggataagata gtttaccctg aagcatttct actgcaattt catgcgttcc
     5581 atttctgcca tattgaggat ttaccttgta cacagaatca cggatagaaa taggcccttc
     5641 tttttcggca ttgaacttta ctgcatagta atgctcgttc atgtatttaa ccacttcggg
     5701 atgaataaat gtgctcgcat ccatgcgttt acaccatcca caccaatcgg tatacatatc
     5761 gatgaatatc ttcttaggtg ctttatcacg tttcgccatc gcatccgtaa aactcatcca
     5821 atgaatgcct tcggtttgag ctggaaggaa tgaagggaga actaaaagca ggaataacaa
     5881 ataccgcata ggattttcaa ttagaagcta aaatacaacc gaattaagga tatcgaaagc
     5941 attcagaaat aaaaaatgcc ggaatacaag agtaaaccgg cattctagaa tttttaatgg
     6001 attacttcac gttacccatt aatttcttta ccatcggtga tataacaatc aatagaacac
     6061 cgcatataac ggcgtaaata gctaattgtt ggtatccacc agtataggaa gttagttttt
     6121 caagagcact tgcattttct cctggtgcag tcatgccagc tccaagtaaa cctgctacat
     6181 attgtccata agcacttgcc aagaaccaca ttcccatcat aacaccttga agcggctgag
     6241 gagatagttt ggtcatgata gacaaaccaa ttggagataa acaaagctca ccaaacgtaa
     6301 tgatgaaata ggcgagtgta aatacttcta aagatgtttt tccttcgcta tcagcaaaga
     6361 aacgagttgc ataaaaaacg aaaaaggcaa gaccaagaaa aaggaatcct aagccaaatt
     6421 tcaccacagt attaggttct tttccacgtt tactaagagc aacccagagt aatccaacta
     6481 aagccgcaaa tgcaattacg aagaatgaat tagccgaatt gtttatccca tttggatcga
     6541 ccgaaatgcc aaaaagttcg ccatgtaagt tgttggttgc gaagatgttt aaggatcctc
     6601 cgctttgttc aaagaatgcc cagaacaaga tggagaagat gatgaaaata agtgcagcga
     6661 agagtttttt acgttcctca acactgtatt tcgacatttc ataaaacaga taaatcaagg
     6721 tacaaggtcc gattgtgtac atgaaataat cggtgtactc ggttttagca accatgacca
     6781 tgattaatgg aatgacaaga agcgaacctg cgtaggttgc atattcaaac cactttttac
     6841 cagttttagt aattggactt aatccaatag gaccgaggct tttttgagtg aaaaggaatg
     6901 tgagcaagct gatggtcatt acgattgcag ctagaccaaa agctagattc caagaataag
     6961 ttttagcaat tgtcacacag gcatatcctc caagtaaggc tccaatgtta atcccggcat
     7021 agaagagaga aaatccagca tcgcgacgac tatcgttcga atgatagagt gcaccaacca
     7081 tagttgagat attcggtttg aagaaaccag ttcccacaat ggtgaaacta atacctacaa
     7141 agaataaatc ttgtggatcg aatgccagaa ttag
//
LOCUS       DAOOQW010000113         7084 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_13036_length_7084_cov_4.699673, whole genome shotgun sequence.
ACCESSION   DAOOQW010000113 DAOOQW010000000
VERSION     DAOOQW010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 7084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7084
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13036_length_7084_cov_4.699673"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3308
                     /locus_tag="PK281_07360"
     CDS             <1..3308
                     /locus_tag="PK281_07360"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOY49201.1"
                     /translation="AVYDYYIREDSSCTFPIVPPQIVSTSDTISACEVAVINIQLGTG
                     LNWQAGPIFNYSPSTSVDQTGMYQYTISQIDGCRTFTDSIYVVIHPSPSQTSIYSTYG
                     TIKTAIPQMGCWEKLTKLYGDTAQLYGNLSSPQNNGAWSDPNGNYTSGTQVSAFDDGI
                     YTFTETTSFGCSFSKCVRVISYTINSQGGIPSNPGDSAVAHVLDLLADLVMDDTINIC
                     EDENIILQLVDSITYYAPLMVPAFVYWEISPNATFSPSNEGGPYTFLDHTNKVKMEES
                     GYLQVKLSMLLPPFNSDTILQVEREVYVNINPNPDFALSITSASNGICPGDTSTLLIT
                     APHPYTLVGNFFSLSSDSSSCLAVQPGYFTLEANYVDPITGCGDHKFDSYHLKVKDAP
                     IVTLNPSSGIKCPADSVTLIADAGNWVSWFGPQGNEIGNAQQINVNPPGNYYYILTDF
                     SNCTLLSNTVEVKDLSSPEILTSPGSICNGNPAYVTLTSDPLIQYNWLPPLSGSDTLQ
                     LVYEPGIYMVEATFCNATNTFSIEVELTGLQAEIGFSTDSVLCTQRDLLLVANSGMVE
                     YSWQNGAFQTPDYLASDSGWVYLSIKDPFGCISRDSIYLEEFTSPLPPESLFTIPSCP
                     GTIATAIAISNLPILWFDDLDQSSFASGDTLLYTNNSSDQFIYAATVDTTTSCTSSRI
                     QTEWVLKSNVIFPFVIDSLNLCGGETINLNMSQVEGLESGSWVGPNGFTSSNLNASIS
                     NADSSMEGSYYFYPIGDELFCVVDTPQITVDIFMVPEPQIDFPESMCENQGFYVEPIL
                     QDNMTYHWTGPNGSFILNSYLSYDELHLNNAGNYTLTSTLEQCSREDQFHLSVVHGPK
                     LNPPFILSSPTCVGNTIYVVNLNPASIAGMTVNWYNAQDFQTLNSDTLYLPNAQVSNS
                     GDFSVQVEANGCLSLMKVFHLVVQENPVSNLIDSYPFCEGDELTILGPPDMYEYNWST
                     GENTQQAHVTEEYTIVLEIVDSHGCSVIDSTFADAVYCDVDKTPNTFSPNDDGINDKL
                     IFKVEGGIIHLATIFDRQGRKIKTLSSPNIDWDGKNESGERVIPATYFYVLQVGMLNG
                     ELKSQEGTITVFE"
     gene            complement(3324..5249)
                     /locus_tag="PK281_07365"
     CDS             complement(3324..5249)
                     /locus_tag="PK281_07365"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY49202.1"
                     /translation="MKKQLCFFAFFFLVFFSNWTFAQSAKPEWLYIIDGYNDTFVSDL
                     EVDKEGNSYLAVNYCGALTVSGLNKKMPPSGHVHGLLIKLNKNGKPLWAHGFKSAYDN
                     RINDLSIAPDGDVLITGFGDGVMSFPGLNDTLKCGREKGKNDYHYPQAVYAARYSPKG
                     ERIWVHFWNTGWAEGMSIAANSKEEVYFSFYHRGSLRNKETLIDSFPVTPKLSAMVSY
                     AHIDKDGKLVKIHHFDFQETSSSAHTPHLKFDKDDNLYMYGAFKGKVKFSEKDSLTND
                     GYYESNDSYLVKFNPAGNLLWKKRIGGQNGQWIKEIQFADDGSIYATGEYSFECTIGD
                     GISLSQKSKYEYKSGDSFMYFKLNADGELVFTRFEEKGNYGTYFTARSIGLDPNGESH
                     IVGYYTDTIQFEGFFLPGISRTNQMFYSRWQEDKLLELENVGNSNRYGLSPMRIGFGP
                     TGQFAVTGVYNDKSCSINIQGQEKKFGKYEYGNATFVYGGSVPVIRKTEPSLLATKTI
                     RFNHLETLKPLLACLKPKESTLPNIWFPTTDSIPSRETWLSESPCGREIVQLEASLFP
                     NPSAGQSTLKLNGMMGGQVQLDVFSENGALLFSKRITVPSEEYLQDLNLSSAAPGIYF
                     VRIVHGGFEKAIRMVKI"
     gene            5355..6983
                     /locus_tag="PK281_07370"
     CDS             5355..6983
                     /locus_tag="PK281_07370"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HOY49203.1"
                     /translation="MLIRKLFIAGFLLILLFPLTAQKHQALIPYRKGKLWGMADTTGK
                     IIVAPKYDYVEFNSVKKKSKYTLPSGYYYVVKDSLLGIISNKEIIPPIHGVIECFEGI
                     LFAAAPNKNSNPVFYNMEGKLVIKDPCTIEQAIWSYENPQTGKPEVVIIKCIQPDNFK
                     AIYCYYVNDPVKSGWLYKDCFSIRYNSSKSDQSIMFVSRGKENPNNEYTQTYVYDFAK
                     KVFMEKYLSTNPKVESQYGSGESGYVGSGRRSDYGDVSVDRAPDLDPGENIAAIQTIS
                     KSFEIKNDSLFCRYMYGDYRSGRAPQFVRNPIQLPEGSTKPEIVKYFSYVTLNEENTR
                     LSRSNAVLFNYKGKKGLLTDEKLEPVYHDSILLFSAAQKGLHHFLVGDKASDGSWRLG
                     TIDEKGQTHLSLEFEQIINFENTPSNTSLTAPFMYLLAKKDNQWGIVNANNEQQLPFA
                     YDSIYLARKSGATDCMVLKKNGKYGLYFDRNHNSDKGFPRQLFEPQFDYQPRFVYHLM
                     REKATANYFLMLLVDEKGNPMGYVDEHGRKFWKD"
BASE COUNT         2144 a         1440 c         1304 g         2196 t
ORIGIN      
        1 gcgctgttta tgattattat attcgagagg attcatcttg tactttccca attgtaccac
       61 cacaaattgt ttccacttcc gatacaattt ctgcctgcga agttgccgtt atcaatatac
      121 aactaggtac tggattaaac tggcaagctg gaccaatctt caattattct ccatcaacaa
      181 gtgtcgatca aacggggatg tatcagtata ccatttctca aattgatggt tgtaggactt
      241 ttaccgattc catttatgta gtcatccatc catcacccag tcaaacaagt atatattcca
      301 cctatgggac aataaaaacg gccataccac aaatgggttg ttgggaaaaa ctgaccaaac
      361 tttatggtga tactgcccaa ttatatggca atctttctag tcctcaaaat aatggtgcat
      421 ggtcagatcc taatggaaat tatactagtg gaactcaagt ttcagccttt gatgatggca
      481 tatacacctt caccgagaca actagttttg gatgctcgtt ctctaaatgt gtaagggtaa
      541 ttagctatac tataaatagt caaggtggca ttccaagtaa tccaggagat tcggctgttg
      601 cccatgtttt agatttactt gccgacttgg ttatggacga caccataaac atttgcgagg
      661 atgaaaacat catacttcaa cttgtcgata gtataaccta ttatgcaccc ttaatggttc
      721 ctgcttttgt ttactgggaa attagtccaa atgcaacttt tagccccagc aatgaaggcg
      781 gtccgtatac ttttttagac cataccaaca aagttaaaat ggaagaatcg ggctatttgc
      841 aggtgaaatt atctatgcta ctgcctccat ttaatagcga caccattttg caagtcgaac
      901 gcgaggtata tgtgaatatt aatcccaatc ccgattttgc tctttcaatt acatctgctt
      961 ccaatgggat ttgtcccgga gacacatcca cattactaat cacagcacct catccatata
     1021 ctctagtagg taatttcttt agcttaagta gcgacagttc aagttgtctg gcggttcagc
     1081 ctggatattt cacactagaa gcaaactacg ttgatccaat aacgggatgt ggtgatcaca
     1141 aattcgatag ctatcacttg aaagttaaag atgctccaat agttactcta aatccaagta
     1201 gtggaataaa atgtcccgcc gattctgtaa cactaattgc agatgccggc aattgggtta
     1261 gttggtttgg tcctcaagga aatgaaattg gtaacgcgca acaaatcaat gttaatcctc
     1321 caggaaacta ctattacatt ttaaccgatt tctcgaattg tacattgctc tccaatacag
     1381 tagaagtcaa agatttgagc tcacctgaaa ttctaacaag tccaggaagt atttgcaatg
     1441 gaaaccctgc ttatgtaaca cttacatccg atccattaat tcaatataat tggctgccac
     1501 cattatcggg aagcgatacg ttacaactgg tttatgaacc cggtatttat atggtagagg
     1561 caacattttg caatgcgacc aacacctttt ctatcgaagt agaattaacc ggattgcagg
     1621 cagagattgg cttttcaacc gattcggtat tatgtactca gcgcgattta ttgttggtag
     1681 cgaattcagg tatggttgaa tattcttggc aaaacggagc atttcaaaca cccgattatt
     1741 tagctagtga ttcgggatgg gtatatttaa gcatcaaaga cccatttgga tgtataagca
     1801 gagattccat ttatctggaa gaatttactt ctccacttcc tcctgaatca ttgtttacta
     1861 ttccatcctg tcccggaaca attgcaacag ctattgcaat cagcaacctg cctattttat
     1921 ggttcgatga tctcgatcaa agttcatttg catcaggcga cacattgctt tatacaaata
     1981 actcaagtga tcaattcatt tatgctgcaa ctgtcgatac aactacatca tgtaccagtt
     2041 ctcgcatcca aaccgaatgg gttttaaagt caaacgtgat ttttccattt gtaatagaca
     2101 gtttaaatct atgtggagga gaaactatca acctaaatat gtcacaagtt gaagggcttg
     2161 aaagtggctc ttgggttgga ccaaacgggt ttacgagttc caatctaaat gcttctatct
     2221 caaatgccga ttcaagcatg gaaggttcct attatttcta tcctattggt gatgaattat
     2281 tttgtgttgt tgatacgcca caaattaccg ttgatatttt catggtacct gagccccaaa
     2341 ttgactttcc tgaatctatg tgtgagaacc aaggctttta tgttgaacca atccttcaag
     2401 ataacatgac atatcattgg actggtccga atggttcgtt tatattaaat tcttatttat
     2461 catatgatga attacatcta aacaacgctg gaaattatac acttacttct acgttagaac
     2521 aatgcagtcg tgaagatcaa tttcatttga gtgtagtaca tggccccaaa ttaaatcctc
     2581 cgtttatttt gtcttctcca acttgtgtag gaaatacgat ttatgttgtt aatctaaatc
     2641 ccgcttcaat cgcaggaatg acggttaatt ggtacaatgc tcaagatttc caaacactaa
     2701 acagcgatac actttactta cctaatgcac aggttagcaa ctcgggcgat tttagtgtcc
     2761 aagttgaagc aaatgggtgt ttatctctta tgaaagtctt tcatctagtc gttcaagaaa
     2821 accccgttag taatttgatc gattcatacc cattttgcga aggtgatgaa ttaaccatat
     2881 taggtccgcc cgacatgtac gaatacaact ggagcacagg agaaaatact caacaagcac
     2941 atgtaacaga agaatatact attgtactag aaatagttga ttctcacggt tgttccgtta
     3001 tcgattccac ttttgcagat gctgtttatt gtgatgtaga taaaactcct aatacgttta
     3061 gccctaatga tgatggtatt aatgacaaat tgatattcaa agtcgaagga ggaataattc
     3121 acctggctac aatcttcgat agacagggaa gaaagatcaa gacattatcc tctccgaata
     3181 ttgattggga tggaaaaaat gaatcgggag agcgcgtcat ccctgcaact tatttttatg
     3241 tgttacaggt tggaatgctc aatggtgaac taaaatccca agaaggaact atcactgtat
     3301 ttgaataaac tggtgcaatt ccttcaaatc ttcaccatcc gaatcgcttt ctcaaatccg
     3361 ccatggacaa tacgtacgaa gtaaattcct ggtgctgcag aactcaagtt taggtcttgt
     3421 aaatattcct cactcggaac tgtaatgcgt tttgaaaaaa gcagtgctcc gttttccgaa
     3481 aacacatcca attgcacttg tccgcccatc attccattaa gttttagagt gctttgtccc
     3541 gcacttggat tgggaaacaa actcgcttct aactgaacta tttctcttcc gcagggcgat
     3601 tcactcaacc atgtttcgcg tgatgggatg gaatcggttg ttggaaacca gatattggga
     3661 agagtgcttt cctttggttt taggcatgcc aataaaggct tcaaggtttc taagtgattg
     3721 aaacgaatcg tctttgttgc caatagtgaa ggttccgttt ttcttatcac aggaacactt
     3781 cctccataca caaacgtggc atttccatac tcgtattttc caaacttctt ttcttgtcct
     3841 tgtatattga ttgaacagct tttgtcattg taaactcctg ttactgcaaa ctgtccagta
     3901 ggaccaaacc caatgcgcat gggactaaga ccgtatcgat tactatttcc tacattttcc
     3961 agttctagta atttatcttc ctgccatcta ctgtaaaaca tctgattcgt cctcgaaatt
     4021 cccggtagga aaaacccttc aaattgaatt gtatcagtat aataaccaac aatatgcgac
     4081 tcgccattcg ggtcaagtcc aatcgatcgt gccgtaaaat aagtgccgta atttcctttc
     4141 tcttcgaaac gggtaaaaac cagttctcca tccgcgttca acttaaaata cataaagcta
     4201 tcgcccgatt tgtattcgta tttgctcttc tgagacagac taataccatc accaatcgta
     4261 cattcaaagc tatattctcc tgttgcgtat atggatccat catcagcaaa ttgaatttcc
     4321 ttaatccatt gaccattttg cccaccaatc ctttttttcc aaagtaaatt tccagcagga
     4381 ttgaatttca ctaaataaga atcgttgctt tcataatatc catcatttgt gagcgaatct
     4441 ttttcactga atttcacttt gcctttgaaa gcaccataca tgtatagatt gtcgtcttta
     4501 tcgaacttca aatgcggagt atgtgcacta ctactcgttt cctgaaaatc gaaatgatga
     4561 atttttacca attttccatc tttatcgatg tgagcatacg aaaccatagc tgaaagcttt
     4621 ggtgttacag ggaaagaatc gattagcgtt tctttatttc tcaaacttcc tcgatgataa
     4681 aaactgaaat acacttcctc tttactattc gcagcaatgc tcattccttc ggcccaacct
     4741 gtattccaga aatgaaccca aatgcgttct cctttaggtg agtatcgagc tgcataaact
     4801 gcttggggat aatggtaatc gtttttccct ttttctcttc cacattttaa cgtgtcattc
     4861 aatccaggaa aactcatcac gccatctcca aatccagtaa tgagtacatc tccatcaggc
     4921 gctatcgaca aatcattaat tcggttatca tacgcacttt tgaaaccatg tgcccaaagt
     4981 ggtttcccat ttttatttag ctttatcaat aagccatgta catgtcccga aggaggcatt
     5041 ttcttattta agccactaac cgttagcgct ccgcaataat taacggccaa ataggagttt
     5101 ccttctttat cgacctccaa atcgctaaca aaggtgtcgt tgtatccatc aataatatat
     5161 aaccattcgg gtttagctga ttgagcaaac gtccaattgg aaaagaaaac gagaaaaaaa
     5221 aatgcgaaaa agcagagttg ttttttcata agataaagga agcgaaagat aagattagca
     5281 ggcactattt gctagttatg aggaaaggtt catttgcatt tgtttcgatt taacttcgca
     5341 gttcataatc caatatgctc atccgaaaac tatttattgc tggatttctt ttgattcttc
     5401 tgtttccttt aacggcacaa aagcaccaag cccttattcc atatagaaaa ggaaaacttt
     5461 ggggaatggc tgacactacg ggtaaaatca tcgtcgctcc taaatacgat tatgttgaat
     5521 ttaattcggt gaagaagaag agtaaataca cgttgccttc aggatattat tatgtggtga
     5581 aagattcttt actaggtatt atcagtaata aagaaattat tccacctatt catggagtaa
     5641 tcgaatgttt cgaaggaatt ctatttgctg cagcacctaa caaaaatagc aatccggttt
     5701 tttataacat ggaagggaag cttgttatca aagatccatg caccattgaa caagctattt
     5761 ggtcgtatga aaatccccaa acaggaaagc ccgaagtggt catcatcaag tgtattcaac
     5821 cagataattt caaagcgatc tactgctatt atgtgaatga ccctgtaaaa tcgggatggt
     5881 tatataaaga ctgttttagc attcgctata attcttccaa aagcgaccaa tccatcatgt
     5941 ttgttagtag aggaaaagaa aatcccaata acgaatatac ccaaacctat gtgtatgact
     6001 ttgctaagaa agtgtttatg gaaaagtatc tcagtactaa tcctaaagta gaaagccaat
     6061 acggcagtgg tgaatcagga tatgttggaa gtggaagaag aagtgattat ggtgatgtgt
     6121 cggttgatag agcgccagat ttggatcccg gcgaaaatat tgctgctatt caaaccatta
     6181 gtaaatcatt cgaaatcaag aacgattcgc tgttttgtcg ctacatgtat ggagattatc
     6241 gaagtggtcg tgcacctcag tttgtgcgta atcccattca gcttcccgaa ggaagtacaa
     6301 agccagaaat tgttaaatac ttcagctacg taacacttaa cgaggaaaat acgcgcttgt
     6361 ctcgctccaa tgcagtgttg tttaattaca aaggcaaaaa aggtttgctt acagatgaaa
     6421 aacttgaacc cgtttatcac gattccattc tcttgttttc ggctgctcaa aaaggattgc
     6481 atcatttcct tgttggtgat aaagcgagcg atggttcttg gcgcttaggg acgattgatg
     6541 aaaaagggca aacacattta tctctagaat ttgagcagat catcaacttt gaaaacactc
     6601 cctccaacac ctctttgacg gctcctttta tgtatctact agcgaaaaaa gacaatcaat
     6661 ggggaatcgt aaatgccaat aacgagcaac aattaccttt tgcttatgac agcatttact
     6721 tagctcggaa atcgggtgca accgattgta tggtcctaaa gaaaaatggg aaatatggtt
     6781 tgtatttcga tcgcaatcat aacagcgaca agggttttcc tcgtcagctt tttgagcctc
     6841 aattcgatta tcagcctcga tttgtatatc acctgatgag ggaaaaagcc acagccaatt
     6901 atttcttgat gcttttagtg gatgaaaaag gaaatccaat gggctatgta gatgaacatg
     6961 gccgtaagtt ttggaaggat taatcctatc cgtatagcac ttgaaaagcc tcttctatct
     7021 ttttcaccgg aataacgcga atgccaaatt tccccgtgtt gatgcctttt aagttgtaat
     7081 ccga
//
LOCUS       DAOOQW010000114         7081 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_13043_length_7081_cov_4.743809, whole genome shotgun sequence.
ACCESSION   DAOOQW010000114 DAOOQW010000000
VERSION     DAOOQW010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 7081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7081
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13043_length_7081_cov_4.743809"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1018)
                     /gene="bioA"
                     /locus_tag="PK281_07375"
     CDS             complement(<1..1018)
                     /gene="bioA"
                     /locus_tag="PK281_07375"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: protein motif:HMM:TIGR00508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HOY49204.1"
                     /translation="MNWSQRDQAVIWHPFTQAQTAAEPIAIVRAEGIWLYAEDGKRYL
                     DGTSSWWVNAHGHAHPYIAEQIHKQASTLEHIIFAGFTHEPAILLAEKLIRLLPGEQA
                     RIFYSDNGSTSVEVALKMALQYHHNRGQNRRKIISFTNAYHGDTFGAMSVGARNAFNA
                     PFEPLLFEVLSFHPPEKGQEESCLAYMEKLLADEQIAAFIFEPLVQGANGMRMHDSET
                     LGHLIELCRSKGVITIADEVFTGFYRTGKRFACNHLSVQPDIMCLSKTLTGGTLPLGV
                     TAAPEFIYEAFLSDDKYKTFFHGHSFTANPIACRAALASLELFDEPTYLSNIQRIVDA
                     QTQFA"
     gene            complement(1015..1626)
                     /gene="bioD"
                     /locus_tag="PK281_07380"
     CDS             complement(1015..1626)
                     /gene="bioD"
                     /locus_tag="PK281_07380"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010522956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HOY49205.1"
                     /translation="MKRYFITGIGTGVGKTVLSAILCEALKADYWKPIQAGDLENSDT
                     VSVKKLVSKTHSIFHPEAYQLLFPLSPHASAEKTGITIDPATIQVPQTTNNLIIEGAG
                     GLMVPLNRKTLYIDLIPALNAEVILVSKNYLGSINHTLLSAESLMLRNIPVRGIVFNG
                     EENPATESIILEMTGFNFLGRIPQVDVIDSDFIQQQAAAFANV"
     gene            complement(1660..2079)
                     /gene="aroQ"
                     /locus_tag="PK281_07385"
     CDS             complement(1660..2079)
                     /gene="aroQ"
                     /locus_tag="PK281_07385"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008464789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HOY49206.1"
                     /translation="MKLHIINGPNLNLLGTREPEIYGSRGFDSFLDELKREFPSIELS
                     YFQSNVEGEIINNLHEHGFTTDGILLNAGGYTHTSVAIGDAVAAIKTPVIEVHISNVF
                     ARESFRHLSYIAPHALGVISGFGLNSYRLALEHFTRK"
     gene            2103..3002
                     /gene="xerD"
                     /locus_tag="PK281_07390"
     CDS             2103..3002
                     /gene="xerD"
                     /locus_tag="PK281_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HOY49207.1"
                     /translation="MDWSSSIKGFKAFLKLERSLSPNSVEAYERDVEKLWQFFEVHGL
                     SVSPENVQPEHLKGLVKWVAELGMQPRSQARMISGLKSFYKYLLLENLIQTDPCDQLD
                     SPKLGRKLPDTLDVQDIDALIGVIDLSKAEGERNRAILETLYSCGLRVSELVNLKISD
                     LHLNNGFVKVVGKGNKERIVPAGSVVKKRLEDYLGQIRIHVPVKMGEEDIVFLNRRGK
                     RLTRVMVFLMIKDLAKKIGLKKKISPHTFRHSFATSLVEGGADLRAVQEMLGHESITT
                     TEIYTHLDREYLRQVITEFHPRA"
     gene            3092..3922
                     /locus_tag="PK281_07395"
     CDS             3092..3922
                     /locus_tag="PK281_07395"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOY49208.1"
                     /translation="MLTPNSRRSDLSFDEIVFENRNHAYGAFLLRKQYDRNILTGFFF
                     SAGLVLTIVSTMYIMGKAHHLSIDDINHKIAKKHEFKIIEMILPEKPDQPKVGEKSVI
                     PPAGSDKMKMEDKAPTPVNEPIKPEEKKKEETDSVAYNPGGNGKKGPIIPEGGGGKEK
                     EVGNGGAGGIKKVEDFVEIMPEFPGGETAMFKYLSNNLRYPTYERETGMNGIVYVSFV
                     VNVDGVIDDITIKRSPSPGFSDEVIRVISKMPNWKAGIQGGQKVPVRYNLPVKFSLKN
                     "
     gene            complement(3998..>7081)
                     /locus_tag="PK281_07400"
     CDS             complement(3998..>7081)
                     /locus_tag="PK281_07400"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOY49209.1"
                     /translation="YSNSGTAILPSQGTYNNLLFTGGSTKNLFNTDILINGNMSISAG
                     IVSNPSNRIVSLKGNFVNTASLTAFQTGTTGSLQLTGSTQSITGASSFGRLTVNGAGV
                     KTLNSSLTITNLITYVNGVITTGANQLTLALGATGTGASALSYVDGNLQKVIAGGTAT
                     RSFEVGDASTYAPVTIQFSGTTNAGGSFTVNTLSGDHPATATSGIDGSKSVNRFWGIT
                     NSGVAGFSSTSITFNFVSGDLDPAVSTSNLSAARYNTSTWTLPTVGTRTSTSLQVTGI
                     TAFGEFQLAEPFSAGITWTGAVNTDWNNPGNWNPNVIPTGSDNVSIPVVTNEPSFLTP
                     GNGIVRNIVLQSGAYINIPTGYQVTVNGNWSGTNAQVTGPGNVLFTSPIATHSGTTTF
                     FGIVSVASGANLNTSNSLALANGASLMHGVGTIGAGGSITGNVRIRRSGANNVLSYNF
                     WSSPITSATTSVLSGNRYMYDPNQATGSDVVGLRDGWIAPGSFMTPGRGYIATGTANA
                     SFIGIPNNGNISYGPLALGTFTNFNLVGNPYPSGINANSFLSANPQILGGAIYFWDDD
                     NTSGTGYAASDYGVYNGVGFVGPNSGKSFNGNIGSCQGFFIQASNTNPVTFTNSMRTT
                     QNSTFFDADMLDRVWVSVTTQNEDYNETLIAFKEDATDSADAQYDATKLRGNTEIAMY
                     SKIGSDDYAIQALSELTVDKTVQLGVEATANGPQTFRLKQVDNLSETVQIILEDTKLG
                     TFHNLRNNPEYIYNYDASTDVLRFRLHFKPEVLMTSATESCVQNDGQIIVNSPSATTW
                     DFSVTNMAGVAVAHNEAFSGSTTVNNLAGGIYFVNLSNQFGSIIHQAIEVASGSPVIA
                     TASSNASVVEIANAEFTFHATAQGASEYTWDFGDGTIVTGMLNPVHVYTAPGTYTVTF
                     IASNATCMDVKTVIVTVRDLTTGLDKIGQSAFSMFPNPANTSTTISLRLPERQSELVM
                     NILDASGKLVKTQTFKNVETQATLQLNVADLIPGVYQILMNGGTYSTSARLNVSR"
BASE COUNT         2199 a         1367 c         1401 g         2114 t
ORIGIN      
        1 ttgcaaattg agtttgtgca tcaactattc gttgaatatt cgataaatat gttggttcat
       61 caaataattc caaagatgct aaagctgccc gacaagctat tgggttggca gtaaatgaat
      121 gtccgtgaaa aaatgttttg tatttatcat cacttaaaaa agcctcataa ataaattcag
      181 gggctgcagt aactcctaat ggtaaagtgc cacctgtaag tgttttcgat aaacacatga
      241 tatcgggttg aacacttaaa tgattgcaag caaaacgttt cccagttcgg taaaatccag
      301 tgaaaacctc atctgcaatg gtaataacac ctttagagcg acataattca attagatgac
      361 cgagtgtttc cgaatcgtgc atgcgcattc cgttcgcacc ttgcaccaaa ggctcgaaaa
      421 taaaggcagc tatttgctca tctgccaata gtttttccat ataagctaga cagctttctt
      481 cttgtccttt ttcgggagga tgaaagctca atacttcaaa cagtaaaggt tcaaatggag
      541 cattaaatgc atttcgcgcc ccaaccgaca tagcaccaaa cgtatcacca tgataggcat
      601 tcgtaaacga aatgattttt cttctattct gccctcgatt atgatgatac tgcaaggcca
      661 tttttaaagc tacttctaca ctcgtagaac cattgtcgga atagaatatt cgagcttgtt
      721 ctccaggcaa aaggcgtatc agtttttcgg ctaataatat ggctggttca tgcgtaaagc
      781 cagcaaaaat tatgtgctcg agtgtggatg cttgcttgtg tatttgttcc gcaatgtatg
      841 gatgcgcatg tccgtgggca tttacccacc aactggatgt tccatctaaa tagcgtttgc
      901 catcttcggc atacagccaa attccttcgg ctctaacaat agcaataggt tcggctgctg
      961 tttgagcttg agtaaaaggg tgccagatta cagcctggtc gcgttgactc caattcatac
     1021 gtttgcaaat gcagcagctt gttgttgaat aaaatccgaa tctattacat ccacttgagg
     1081 aattcgtccc aagaaattaa atccagtcat ttccaaaata atagattctg tagccggatt
     1141 ttcttctccg ttaaatacaa ttccccgaac agggatgttg cgtaacatca agctttcggc
     1201 acttaataat gtatgattta tacttcctaa ataattcttt gaaaccaaaa ttacttctgc
     1261 atttaaagca ggaattaaat cgatatacag tgttttacga tttaatggaa ccattaatcc
     1321 acctgcacct tcaattatta aattattggt tgtttgaggg acctgaatag tagctggatc
     1381 aattgttatt cctgttttct cggccgatgc atgtggtgag agaggaaata gcaattgata
     1441 ggcttcggga tggaaaatgg aatgtgtttt tgaaactaat ttcttgactg aaacagtatc
     1501 ggaattttcg aggtcaccag cttgtatggg tttccaataa tcggctttca gtgcctcaca
     1561 taaaatagca ctcagcactg ttttgccaac acccgttcca attcctgtaa taaaatagcg
     1621 tttcattttc tagtatttcc gatagggatt tctatcggtt tattttcgag taaaatgttc
     1681 tagggcaagt cgatagctat tcaacccaaa accactgata acacctaatg catgcggagc
     1741 aatgtagctt aaatgccgaa agctttctcg tgcaaaaacg ttactaatat gcacctcaat
     1801 tacgggtgtt tttatagccg ccacagcatc acctatagca acagaagtgt gcgtatatcc
     1861 acctgcattt agcaagattc catccgttgt aaagccatgt tcatgtaggt tattgatgat
     1921 ctcaccttca acattacttt ggaaatagct tagttcaatg gatggaaatt ccctttttaa
     1981 ctcatctaaa aatgaatcaa atccacgtga gccatatatt tcaggttcgc gcgttcccag
     2041 aaggtttaaa tttggaccgt tgatgatatg tagtttcatc cggcaacgaa agtacacaag
     2101 aaatggactg gtcctcctcg attaaaggtt tcaaagcatt tctaaaactc gaacgttcgc
     2161 tttcacctaa ttcggttgaa gcatacgagc gggatgtaga gaaattatgg caattctttg
     2221 aggttcatgg tttatctgtt tctcccgaaa atgtgcagcc cgagcactta aaaggattgg
     2281 tcaaatgggt tgcagaatta ggaatgcaac ctcgatcaca agcccgaatg atttctggat
     2341 tgaagtcgtt ttataaatac ttgttgctcg agaacctcat tcaaaccgat ccttgcgatc
     2401 aacttgactc tcccaaactt ggacgtaaac ttccagatac gttagatgtt caagatattg
     2461 atgcacttat tggtgtaatc gacttgagca aagctgaagg agaaagaaat cgagcaattc
     2521 tcgaaacctt atacagttgc ggattacgtg tatctgaatt ggtgaatctc aaaatttcgg
     2581 atttgcactt aaacaacgga tttgtcaaag ttgtggggaa aggaaataaa gagcgaattg
     2641 tcccggcagg ttcggttgta aagaaaagat tagaagatta tttaggacaa atacggattc
     2701 atgttcccgt taaaatgggc gaggaggata tagttttctt gaatcgtcga ggaaaacgat
     2761 taactcgtgt gatggtattt ttgatgatta aggacttggc taagaaaatt gggcttaaga
     2821 agaagattag tccgcataca ttccgccatt cctttgctac atccttagtg gaaggtggtg
     2881 ctgatttacg agcagttcag gaaatgttag gtcacgaatc cattactaca actgagattt
     2941 atacccattt agatcgagaa tatctccgcc aggtaattac cgaatttcat cctcgcgctt
     3001 gagcgataaa tcccaaaatc tgttctcaat taaacatatc gtttggtgaa ccacttgcgt
     3061 tagctcacat atacgcaaaa aaccaaacgt tatgctgact cccaattccc gccgttctga
     3121 tctctcgttt gatgagatcg tttttgaaaa tcgcaatcat gcttacggag cttttttact
     3181 tcgtaagcag tatgatcgta acattctaac aggttttttc ttttctgccg gattagtact
     3241 tacgattgtt tcgaccatgt atataatggg taaagctcat catttatcta tagatgacat
     3301 taaccataaa atagccaaga aacatgaatt taaaattatt gaaatgattc ttccagaaaa
     3361 gcctgatcaa ccaaaagtag gtgagaaaag tgttattcct ccagccggat cagataaaat
     3421 gaaaatggag gataaagcac caactccagt taacgaacca atcaaacctg aggaaaagaa
     3481 aaaagaagaa accgattcgg tagcgtataa tccgggagga aatggaaaaa aagggccaat
     3541 tattcctgaa ggtggcggag gaaaggaaaa ggaagtagga aatggtggtg caggtggtat
     3601 taaaaaagtg gaggattttg ttgagatcat gcctgaattt cctggaggag agactgccat
     3661 gttcaaatac ctcagtaata atttacgtta tccaacttat gaacgagaaa ctggaatgaa
     3721 tggaattgtt tatgtttcgt ttgtagtaaa tgtggatggg gtgattgatg atattactat
     3781 taaacgttca ccatcgccag gtttttcgga tgaagtaatt cgagtaatta gtaaaatgcc
     3841 taattggaaa gcaggtattc aaggtggtca aaaagtacca gttcggtata atttaccagt
     3901 taagtttagt ttgaagaatt gatcaattaa ttagaaacag aaagccccgt gagaaataac
     3961 tctcacgggg cttttttttg actgaatcaa tacagaatta gcgacttaca ttcaatcgtg
     4021 cagaagtact ataagttccg ccattcatca atatctgata aactcccgga atcaagtctg
     4081 ccacattcaa ttgaagtgta gcttgagttt ctacgttttt gaacgtttga gttttaacca
     4141 gttttccact agcatctaag atattcatga ccagttctga ttgacgttca ggtaaacgca
     4201 agctaatagt tgtacttgtg tttgctggat tagggaacat cgagaatgca gattgaccaa
     4261 ttttatccaa acctgtagtc aaatcgcgca cagtaacaat aactgttttc acgtccatac
     4321 acgttgcatt agaagcgata aacgtaacag tgtaggttcc aggtgctgtg taaacgtgaa
     4381 ccggatttag cattccagtt acaattgttc catcaccaaa atcccaagtg tattcactag
     4441 caccttgtgc tgtagcatgg aaagtaaatt ctgcatttgc gatttctaca actgaagcat
     4501 tagaagaagc agttgcaata actggagaac cacttgccac ttcgattgct tgatggatta
     4561 tacttccaaa ttgattactc aagttaacga aatagattcc tccagctaaa ttattaactg
     4621 ttgtagatcc actaaatgcc tcgttgtgag ccacagcaac acctgccata ttggtaaccg
     4681 agaaatccca agtagtggcc gaaggactat ttactatgat ttgtccatca ttttgtacgc
     4741 aactttcggt agcagaagtc attaaaactt ctggtttgaa atgtaatcgg aaacgaagca
     4801 catcagttga agcatcataa ttatagatgt attcaggatt atttctcaag ttatggaaag
     4861 tacccaattt ggtgtcttca aggatgattt gaactgtttc agataagtta tcgacttgtt
     4921 tcaaacggaa tgtttgaggt ccatttgctg tagcttcaac acctagttga acagttttat
     4981 cgacagttaa ttcggataat gcttgaattg cataatcatc tgaaccaatt ttagaataca
     5041 tcgcaatttc tgtattccca cgaagtttcg ttgcatcata ctgagcatct gcactatcgg
     5101 tagcatcttc tttgaatgcg ataagtgttt cgttgtaatc ttcgttttgt gtcgttacac
     5161 ttacccaaac acgatcaagc atgtctgcat cgaagaaagt actattctga gttgtacgca
     5221 tcgaattagt gaatgtaacc gggttggtat ttgaagcttg aataaagaag ccttggcaag
     5281 aaccaatgtt tccgttaaac gacttgccgc tgttagggcc aacaaatcca acaccattgt
     5341 aaacaccata atcagaagca gcataaccag ttccggaagt attatcatca tcccagaaat
     5401 agatagcacc acccaaaatt tgcggattgg cagataagaa gctattggca ttaataccgc
     5461 tagggtatgg atttccaact aagttaaaat tagtaaacgt acccaaagct agaggaccat
     5521 aagagatgtt cccattgtta ggaataccaa tgaatgaagc atttgcagtt cctgtcgcaa
     5581 tatatccacg acctggtgtc atgaagcttc caggagcaat ccatccatca cgtaaaccaa
     5641 ctacatcaga accagtagct tgatttggat catacatata acgattgcca cttaaaacag
     5701 aagttgtagc actagtaatt ggagatgacc agaaattgta gctcaagaca ttattagcac
     5761 cacttcgacg aatacgtacg tttccagtga tagatccacc agcaccgatt gttccaactc
     5821 catgcatcaa agatgctcca ttagcaagtg ctaatgaatt acttgtgtta agatttgctc
     5881 ctgaagcaac cgatacaatt ccaaagaatg tagttgtacc agaatgtgta gcaattggag
     5941 atgtaaacaa tacattacct ggaccagtaa cttgagcatt tgtaccagac caatttccat
     6001 ttactgttac ttgatatcca gttggaatat tgatataagc accgctttgt agtactatat
     6061 ttctaactat tccatttcct ggagttaaaa acgatggctc attcgttact actggaattg
     6121 aaacattgtc agaaccagta ggaatcacat ttggattcca gtttcctggg ttgttccagt
     6181 cagtgttaac tgctccagtc caagtaatac ctgctgaaaa tggttcagct aattggaatt
     6241 caccaaatgc agtgatacca gtaacttgaa gactagttga agtacgagta cctacagttg
     6301 gtaaagtcca agttgatgtg ttatatcgag ctgcagaaag gttacttgta ctaacagctg
     6361 gatctaaatc acccgaaaca aaattgaatg taatgcttgt ggaagaaaaa ccagcaacac
     6421 ccgagtttgt aataccccaa aatctgttta ccgatttaga accatcgatt ccagatgttg
     6481 cagtcgctgg gtgatcacca cttaacgtat ttactgtaaa tgatccacct gcattagtag
     6541 ttcctgagaa ttgaatagtt actggtgcat atgtacttgc atcaccaact tcaaaactac
     6601 gagttgcagt tccgccagct attacttttt gtaagttacc gtctacataa ctcaaagcag
     6661 aagcaccagt tcctgtagca cctaaagcaa gagttaattg attagcacct gtagtaataa
     6721 ccccatttac ataagtaatc aaattggtaa ttgttaacga actgtttagg gtttttacac
     6781 cagctccatt caccgttaat cgaccaaaag aagaagcgcc agtaatactt tgagtactac
     6841 ctgtaagctg taatgatccg gttgttcccg tttggaaagc tgttaaagaa gcagtattaa
     6901 cgaagttgcc ttttaatgat acaattcggt tgctaggatt gctcacaata cctgcactta
     6961 tggacatgtt accattaatg aggatgtcgg tattgaagag atttttagta cttcctccag
     7021 taaatagaag gttgttatat gttccttgcg aaggtaaaat agcagtaccc gagttactgt
     7081 a
//
LOCUS       DAOOQW010000115         7047 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_13115_length_7047_cov_4.492563, whole genome shotgun sequence.
ACCESSION   DAOOQW010000115 DAOOQW010000000
VERSION     DAOOQW010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 7047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7047
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13115_length_7047_cov_4.492563"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="PK281_07405"
     CDS             complement(<1..211)
                     /locus_tag="PK281_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007094990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOY49210.1"
                     /translation="MAHDLQYTRNIGIAAHIDAGKTTTTERILYYTGVNHKIGEVHDG
                     AATMDWMVQEQERAITITSAATTCFW"
     gene            complement(236..703)
                     /gene="rpsG"
                     /locus_tag="PK281_07410"
     CDS             complement(236..703)
                     /gene="rpsG"
                     /locus_tag="PK281_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006795814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HOY49211.1"
                     /translation="MRKTTPKRIARTPDPRFNDPLVTRFVNNLMFDGKKSIAYSIFYD
                     AVDIVEKRTEENGHEVWKRALENVSPTVEVKSRRVGGATFQIPMEIRPDRRIALGMKW
                     MIMYSRKRNEKSMAQRLASEIVAASKGEGSAHKKKEDTHRMAEANKAFSHFRF"
     gene            complement(732..1145)
                     /gene="rpsL"
                     /locus_tag="PK281_07415"
     CDS             complement(732..1145)
                     /gene="rpsL"
                     /locus_tag="PK281_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HOY49212.1"
                     /translation="MPTIQQLVRKGRTPNASKSKSPALDSCPQRRGVCTRVYTTTPKK
                     PNSAMRKVARVRLTNTKEVNAYIPGEGHNLQEHSIVLIRGGRVKDLPGVRYHIVRGAL
                     DTSGVEGRNQRRSKYGTKRPKPGQPAAAAGKGKKK"
     gene            1567..3927
                     /locus_tag="PK281_07420"
     CDS             1567..3927
                     /locus_tag="PK281_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014034530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOY49213.1"
                     /translation="MSHDYLNELNPIQRQAVENTEGPVMIIAGAGSGKTRVLTYRIAH
                     LLSKGVDAFNILSLTFTNKAAREMKERIHKIVKTTESANLWMGTFHSVFARILRFEAE
                     KLGYPSNFTIYDTDDSKSLIKTILGEMQLDDKIYKPSLVLNRISNAKNNLISSAQYLA
                     HPEVLEEDRQSGKPKLGLVYEAYEKRCFKASAMDFDDLLFKTNVLLKQYPDVLYKYQQ
                     KFKYILVDEYQDTNFSQYLIVKKLSAMYQNICVVGDDAQSIYAFRGANIQNILNFEKD
                     YPDLKVYKLEQNYRSTKTIVQAANSVIRNNKDQLKKVVWTDNKDGELIRLLKASTDNE
                     EGSMVANGIFDLRMNYQLPNSDIAILYRTNAQSRSMEEALRKRGIPYRIFGGLSFYKR
                     KEIKDLLAYCRLTINPNDEEALKRVINYPARGIGKTSLEKIIVSADQHNKSIWEIIEG
                     MSLYRPISGASAQKVIDFVTMIKSFQHGLKSTNAFDLATHIAKSSGLLKELSIDKTPE
                     GISKHENVMELLNGIQEFVDTPPESDDEVAERTLDVFMQDIALLTDADENEKDDPNRV
                     SLMTIHAAKGLEFPAVFVVGLEENLFPSQLSLNSREELEEERRLFYVAITRAEKYLTL
                     SSATTRYRWGQITHSEPSRFLEEIDAALLDMPVAAEPKGFLDFSSERSSFGGFAPKGT
                     ANAFKPKSSPSSDLPVIQPKKGFTPLKKLTDKPIAKAETTSSFIADDVSKLQSGMEVI
                     HERFGPGKVLNVEGRPGEQKATIFFKEVGQKQLLLKFARLKIINNS"
     gene            4005..4652
                     /locus_tag="PK281_07425"
     CDS             4005..4652
                     /locus_tag="PK281_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008464767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4290 domain-containing protein"
                     /protein_id="HOY49214.1"
                     /translation="MEYNSQRPHLIISEYGRHIQRMIDHTCEIADREERNKMARYIIS
                     VMGQLNPHLRDVSDFKHKLWDHLFIMSDFRLDVDSPYPIPSRESFQTKPDRIPYPNAK
                     IRFRHYGKIIEKVIEESKNIEEGPIREALIGAIAYNLKKSYLTWNRDSVQDEQIFKDL
                     SELSGGLLKPAEGTTMTVVAEGVSQNYPQQQGKKFNRGRQKQKNFKRKFGGGTSF"
     gene            4659..5963
                     /gene="murA"
                     /locus_tag="PK281_07430"
     CDS             4659..5963
                     /gene="murA"
                     /locus_tag="PK281_07430"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006257040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOY49215.1"
                     /translation="MNRFEIKGGKRLQGEIIPQGAKNEALQILCAVLLTSQRCVIHNV
                     PDIVDVNKLIELLGALGVKVEKLGNGSWAFTADQVDVDFMVSEEFKKKGSALRGSVMI
                     VGPLLTRFGRGYIPKPGGDKIGRRRMDTHFIGFQSLGASFDFDGVESFYKVEAKELKG
                     AFMLLDEASVTGTANILMAATMAKGTTTIFNAACEPYIQQLCKMLNSMGAKISGVGSN
                     LLTIEGVKELGGCEHRMLPDMIEIGSFIGLAAMTQSNIRIKDTGYDHLGMIPDVFRRL
                     GIELFRDGDDIVIPEQDSYTINKYIDGSILTVSDSPWPGFTPDLLSIVLVVATQANGS
                     VLIHQKMFESRLFFVDKLIDMGAQIILCDPHRATIIGLNRKYKLRGIRMTSPDIRAGV
                     SLLIAALSAEGTSIIDNIEQIDRGYQNIDARLQALGADIKRF"
     gene            5974..>7047
                     /locus_tag="PK281_07435"
     CDS             5974..>7047
                     /locus_tag="PK281_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49216.1"
                     /translation="MISRFLHISTSNLLILFLFIAQWQILFVRLDNDRIKNVIGSDGS
                     GYYEYLPKTFIKKDYPFALPGMVKKEEVKPEQLISITGGPVNKYFFGESLALLPFFLA
                     ADLLTQSFRPDMRDGYSSYYQISVSIAALFYLFLGLWMIHRLLRRMNYAQEIINLILI
                     LLFLGTNLYYYALQEPSMSHVYSFALIATFIDQLHLQLQSSKPIRWLFLSFLLSFIVF
                     IRPVNIVVILVLFPLAGSFSQSISCIKDLFSKPAIWLTSLLIPFFFLGIQATFYYVQA
                     GHWWVDSYGEEGFNFSKPEIVNVLFSFRKGLFIYTPLLLLLIPGLYFYKKRFGLFSLF
                     SWSLLLVISIYIISSWWYWAYGGS"
BASE COUNT         2038 a         1361 c         1439 g         2209 t
ORIGIN      
        1 tccaaaagca ggttgtagct gctgatgtga ttgtgatagc gcgttcttgc tcctgaacca
       61 tccagtccat agtagcagca ccatcgtgta cctcaccaat tttgtgatta actccggtgt
      121 aataaaggat acgctcagtc gtagttgttt taccagcatc aatgtgcgcg gcaataccga
      181 tgttgcgtgt gtattgaaga tcgtgagcca tgacgttctt gggaatctct tgggattaga
      241 agcggaaatg tgaaaatgct ttgttcgcct cagccatacg gtgagtgtct tctttctttt
      301 tgtgagcgga accttcgcct ttcgacgcag ctacaatctc agaagcaagg cgttgtgcca
      361 ttgacttctc attgcgctta cgtgaataca taatcatcca cttcatacct aaagctatac
      421 gacgatccgg acggatttcc atcggaatct ggaacgtagc tcctccaaca cggcggcttt
      481 taacctctac tgtaggactt acgttctcca aagcacgttt ccatacttcg tgaccgtttt
      541 cttcagttcg tttttctacg atatcaactg catcgtaaaa aatggagtaa gcgatgctct
      601 ttttaccatc gaacatcagg ttgttaacga aacgcgttac caaaggatcg ttgaatcgtg
      661 gatccggcgt gcgggctatt cgttttgggg tggtctttct catggtgcga aaggaatgct
      721 attatctgaa attatttctt tttgcctttt cctgcagcag ctgccggttg tcctggtttc
      781 ggacgttttg ttccgtactt agaacgacgt tgattacgac cttctactcc agatgtatct
      841 aacgctccac gaacaatgtg gtaacgtacc cctggaagat cttttacacg accaccgcgg
      901 atcaaaacga tactgtgttc ctgtaagtta tgcccttcac ccgggatata agcgttaact
      961 tctttcgtat tcgttaaacg aacacgagct actttacgca ttgccgagtt tggcttcttt
     1021 ggtgttgtgg tatatacgcg tgtacacact cctctacgct gaggacatga atccaatgct
     1081 ggactcttac tctttgatgc gttaggcgtg cggcctttac gtacaagctg ctggatagta
     1141 ggcatgcttt tagacgttga ttgtatggat gttagagact ctttgttgct attttgccca
     1201 atttttggga gcgcaaagat aggaagtact ttcaaattca caactaggtg agtaaaaaat
     1261 aattccacaa catcctgctt ttcttttcaa catattttaa gtttgcctgt ttcatctgtc
     1321 acaactagcc ctgtatatcc ctacagcact cgatttgtga actttactgc tagttatctt
     1381 tccagcacta aattgaatta attattttta atggggcgcc ttcggtcggg tgctctcctg
     1441 tacgcttcgc cccaatgggg ctgcagggta cttgtttcgc cccctcgccc agcatcatct
     1501 ttttttcaac cagtacgttt atattttact tctcatcttc tttcctgcat tacctacttt
     1561 tgcaccatgt cacacgatta tttgaacgaa ttaaatccta ttcagcggca agcagtcgaa
     1621 aataccgaag gccctgttat gattattgcc ggtgctggat caggtaaaac tcgagtcctt
     1681 acatatcgta ttgcccacct acttagtaaa ggtgtggatg cattcaatat tctttcgctc
     1741 acgttcacca acaaagctgc tcgtgaaatg aaagaacgta ttcataaaat cgttaaaaca
     1801 accgaatcgg ctaatctatg gatgggcacc tttcactctg tatttgcccg tattcttcga
     1861 tttgaggcag aaaaacttgg atatccttcc aattttacca tctatgatac cgacgattcc
     1921 aaaagcctca tcaaaaccat tcttggtgaa atgcaattgg atgataaaat atacaagcct
     1981 tctttagttc tcaatcgaat ttcgaatgcc aaaaataatt taatatcctc tgctcagtac
     2041 ttagctcatc ccgaagtact cgaagaagat cgccaaagcg gtaagcccaa acttgggttg
     2101 gtatatgaag cctacgaaaa acgttgcttc aaagcttccg caatggattt tgatgatttg
     2161 ttattcaaaa ccaatgtgct attaaagcag tatcctgatg tactatataa gtatcaacag
     2221 aaattcaagt acattttagt agatgagtac caggatacca acttcagcca atacttaatt
     2281 gttaagaaac tgtcagccat gtaccagaat atatgtgtag ttggcgatga tgctcagagt
     2341 atttatgctt tccgtggtgc caatattcag aacatcctca atttcgaaaa ggactatcct
     2401 gatttgaagg tttataaact cgaacaaaat tatcgttcaa caaaaactat cgttcaggct
     2461 gctaatagtg tcattcgaaa caataaagac caattaaaaa aagtagtttg gaccgacaat
     2521 aaagacggag aattaattcg tctgttaaaa gcaagtaccg ataatgaaga aggatcaatg
     2581 gtggcaaacg gcatcttcga tcttcgaatg aattatcaat tacccaatag cgatattgca
     2641 attctctatc gaacgaatgc tcaaagtcgt tcgatggaag aggcgcttcg aaaacgtgga
     2701 attccttatc gaatctttgg gggactttcg ttttataaac gcaaggaaat taaagatttg
     2761 ctagcctatt gtaggcttac tattaatccc aacgatgagg aagcattgaa acgagtgatt
     2821 aattatcctg ctcgtggaat tgggaaaacc tcattggaga aaattatcgt ttcggccgat
     2881 cagcacaata aaagtatctg ggagattata gaaggaatgt cgctatatcg tcctatcagt
     2941 ggagcttcag cacaaaaagt aattgatttt gtgaccatga ttaagagctt tcaacatgga
     3001 ctcaaatcaa ccaatgcttt tgatttagct acacatattg caaaatcatc cggactttta
     3061 aaagagttat ccatcgataa aacacctgaa ggaattagca agcatgaaaa tgtgatggaa
     3121 ttgctaaacg gtattcagga atttgtggat actccacctg aaagtgatga tgaagtggca
     3181 gaacgaacct tggatgtatt catgcaagat attgctttgc ttaccgatgc agatgagaac
     3241 gaaaaagacg atcccaatcg agtatcctta atgaccattc acgctgcaaa aggattggaa
     3301 tttcctgcag tatttgtggt tggtttagaa gagaatttat ttccatctca actttcttta
     3361 aatagtcgcg aagaattaga agaagagcgc cgtttatttt atgtagcaat tactcgggct
     3421 gaaaaatact tgactctttc aagtgctact acacgttatc gttggggaca aattactcat
     3481 tcggaaccaa gtcggttttt ggaggaaatt gatgccgctt tattagatat gcctgttgct
     3541 gctgagccta aaggattttt agatttcagt tcagagcgtt ccagtttcgg tggatttgct
     3601 cctaaaggaa cggccaatgc tttcaaacca aaatcatccc caagctctga tttgcctgta
     3661 attcaaccga aaaagggatt tactccatta aagaagttaa ccgacaagcc aatcgctaaa
     3721 gcagaaacta catcatcttt tatagctgat gatgtaagta agcttcaatc gggtatggag
     3781 gtaattcacg aacgatttgg tcctggtaag gtcttaaatg tagaaggaag accaggagaa
     3841 cagaaagcaa ccatcttttt taaagaagta ggacaaaaac aattattgct taagtttgca
     3901 cgacttaaga taataaacaa ctcctgattc tctttatagc gagatattta gcaggacttt
     3961 ccggattcat ttaaaaaccg gttttttccc acgtataata aagcatggaa tacaattccc
     4021 aaagaccaca tctcattatt tctgagtacg gtcgccacat tcaacgtatg attgatcata
     4081 cttgtgaaat tgccgatcgg gaagaacgta ataaaatggc acgttacatt atttctgtaa
     4141 tgggtcaact caatccacat ttaagagacg tgagcgattt caaacataag ctttgggatc
     4201 acttgtttat catgtctgac ttccgattag atgtagattc tccttatcct attccatcaa
     4261 gagagagttt tcaaaccaaa ccggatcgaa ttccatatcc aaatgcaaaa attcgttttc
     4321 gccattatgg aaagattatc gagaaggtaa tcgaagagtc taaaaatatt gaagaagggc
     4381 caattcggga agcgttaatt ggagctatcg catacaactt aaagaaatca tatttaacct
     4441 ggaaccgtga ttcggtgcag gatgaacaga tattcaaaga cttaagtgaa ttgtccggag
     4501 ggctactaaa accagccgaa ggaacaacca tgacagtcgt agcagaaggt gtttcgcaaa
     4561 attatccaca acaacagggt aagaagttta atcgcggacg tcaaaaacag aaaaacttca
     4621 aacgtaaatt cggcggcgga accagtttct gacgttgtat gaatcgattt gaaattaaag
     4681 gaggcaaacg ccttcaggga gaaattatcc cacaaggtgc aaagaatgaa gctttgcaaa
     4741 ttttgtgtgc cgtattgcta acatctcagc gttgtgtcat tcacaatgtg cctgatattg
     4801 ttgatgtaaa caagctcatt gagttattag gcgcactcgg agttaaagtc gaaaaactag
     4861 gaaatggatc ttgggcattt actgctgatc aggtagatgt cgatttcatg gtctccgagg
     4921 aattcaagaa aaaaggttct gctcttcgtg gttctgtcat gattgttggt cctttgctaa
     4981 cacgatttgg tcgtggttat attcctaaac cgggtggaga taagattggc cgccgccgca
     5041 tggataccca ttttattggt ttccaaagtc taggtgctag tttcgatttt gatggagtag
     5101 aaagtttcta taaggtagaa gcgaaagagc ttaaaggagc atttatgttg cttgatgagg
     5161 catccgttac cggaactgcc aatattctga tggcagcaac tatggccaaa gggacaacta
     5221 ccatttttaa tgctgcttgt gagccgtata ttcagcaatt atgcaagatg ctgaatagta
     5281 tgggggctaa gatctcgggt gttggatcta atttacttac cattgaaggt gttaaagaat
     5341 taggtggatg cgagcaccgt atgttgcccg atatgattga aataggatca ttcatcggtt
     5401 tagcagcaat gacccaaagt aacattcgaa ttaaggatac aggttatgat catctaggaa
     5461 tgattccaga tgtgtttcgt aggcttggga ttgagttatt tcgcgatggg gacgatatcg
     5521 ttattccaga acaagatagt tacaccatca ataaatacat cgatggttca atcctaacag
     5581 tgtcggattc tccttggcca ggctttacgc ccgatttgct cagtattgtt cttgtggtag
     5641 caactcaagc caatggaagt gtgctgattc atcaaaaaat gtttgaaagc cgtttattct
     5701 tcgtcgacaa actgattgat atgggcgctc agattattct gtgtgatccg catcgtgcta
     5761 ccattatcgg actaaaccgt aagtataaac tacgtggaat tcgaatgaca tctcctgata
     5821 ttcgtgcagg tgtttcctta ctaattgcag cattatctgc cgaaggaaca agtattattg
     5881 ataatatcga gcaaattgat cgaggatatc agaatattga tgcccgtttg caggcattag
     5941 gagccgatat caaacgattc taatcattag aacatgatca gcaggttttt acatatttca
     6001 acgtctaacc tgctgatact gtttttattc atcgctcagt ggcagatatt atttgtcagg
     6061 ctcgataacg atagaataaa gaacgttatt ggatctgatg gttcgggcta ttatgaatat
     6121 ttgcctaaga cttttataaa gaaagactat ccttttgctt tgcccggaat ggttaagaaa
     6181 gaggaagtaa aaccagagca attaatttcg attactggag gaccagttaa taaatacttc
     6241 tttggagaat cattagcctt attgccattt tttctcgcgg ctgatttact tacccagtct
     6301 tttagacctg atatgcgtga tggttattca tcctattatc agatttcagt ttcgattgcg
     6361 gcattattct atttgtttct tggtttatgg atgattcatc gcctgttaag acgtatgaat
     6421 tatgctcaag aaatcatcaa cttaatactg atattacttt ttttaggaac caatctgtat
     6481 tactacgcat tgcaggaacc tagcatgtcg catgtgtatt cctttgcgct tattgctacg
     6541 ttcattgacc agcttcattt acaattacaa tcttctaagc ctattcgttg gttgttttta
     6601 tcattcttac tctcatttat tgtatttatt cgacctgtta atattgttgt aattttagtt
     6661 ttatttccct tagcaggctc attcagccaa agtattagtt gcattaaaga cctgttttct
     6721 aaacctgcaa tttggctaac gagtttgcta attcctttct tctttttagg gattcaagct
     6781 acattctact atgtacaagc tggacattgg tgggttgata gttatggtga agaaggattt
     6841 aattttagta aacccgaaat tgttaatgta ttattcagtt tccggaaggg tttattcatt
     6901 tatacgccac ttctactatt acttattccg ggattgtatt tttataagaa aaggttcggt
     6961 ttatttagcc tatttagctg gtcattatta ctagtaattt caatttatat catttcttct
     7021 tggtggtatt gggcatatgg aggaagt
//
LOCUS       DAOOQW010000116         7031 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_13160_length_7031_cov_4.159547, whole genome shotgun sequence.
ACCESSION   DAOOQW010000116 DAOOQW010000000
VERSION     DAOOQW010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 7031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7031
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13160_length_7031_cov_4.159547"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1726
                     /locus_tag="PK281_07440"
     CDS             <1..1726
                     /locus_tag="PK281_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOY49217.1"
                     /translation="LEASFIYEDTPDQVKATRDVKRDMEKDAPMDRLVCGDVGFGKTE
                     IAVRAAFKAVNDSKQVAILVPTTILALQHYKTFAERLSQLPCTVDYINRFKSPKEQKE
                     TLERLAKGEIDILIGTHRIISKDVKFKDLGLMIIDEEQKFGVAAKEKLKQIKVNVDTL
                     TLTATPIPRTLHFSMMGARDLSVINTPPPNRHPVQTELHTFNEELIRDAVTFEVQRGG
                     QVFFVHNRVQNIQEVGDMIRRLCPGVRVCCGHGQMEPEELENVMMRFIDGDYDVLVAT
                     TIIESGLDISNANTILINNAHLFGLSDLHQMRGRVGRSNKKAFCYLLTTPVSLLTSDA
                     RKRLKAIEDFAELGSGFNIAMRDLDIRGAGDLLGGEQSGFISEIGFEMYNKILDEAIQ
                     ELKESDFKQLFHEELEEKRKVWVSDCQIDTDLEILIPDAYVTNITERLLLYKDLDNME
                     TEVELQQFEKQLTDRFGAVPKPTLELMNAIRLRWIAKDMGFEKVVLKQKTFVGYFVQN
                     QQSSFYQSDFFARLMQHLTRIPGKARLKERNNKLTLVMEQTGNLDQAVNALGQLLVAT
                     RPQEQTLN"
     gene            1726..2436
                     /locus_tag="PK281_07445"
     CDS             1726..2436
                     /locus_tag="PK281_07445"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HOY49218.1"
                     /translation="MHVFYTTTFQGDKAILTEEESLHLSKVLRLKEGASVLLLDGKGG
                     MFEGEVLLVHKKNSAIGNLRRLPVTNSRPYRLHVAIAPTKMMERLEWFLEKATEIGID
                     EITPLITNRTERDQVKMQRVEKIVLAAMKQSMTATMPVLNEVTTFATFLKKETSGLKL
                     IAHCIPGEKPLLTQKASQADQITILVGPEGDFTAEEVGSALKVGYEAISLGQSRLRAE
                     TAGVVVCAQVQASWQIKA"
     gene            2472..3146
                     /locus_tag="PK281_07450"
     CDS             2472..3146
                     /locus_tag="PK281_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HOY49219.1"
                     /translation="MKRFLILVLTVLSVTSIQAQGTYSIGLLKYNGGGDWYANPTSLT
                     NLISFCNKNIGTNISPDAITVDAGNPEIFNLPFVHMTGHGNVIFSPSESDNLRTYLIA
                     GGFLHVDDNYGMDPFIRPQLKRIFPELDLVELPPSHPIFHQKFDFPGGLPKIHEHDGT
                     APQAFGMFWEGRLVVLYTFECDLGDGWEDASVHKDSESARQKALKMGANMLQYVLMAN
                     DPIKRQ"
     gene            complement(3206..4072)
                     /gene="ygiD"
                     /locus_tag="PK281_07455"
     CDS             complement(3206..4072)
                     /gene="ygiD"
                     /locus_tag="PK281_07455"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HOY49220.1"
                     /translation="MKRRKFLYATSISLMGASLVSLRELASGPKSSERMPVLFVGHGN
                     PMNAIEKNKYSLAWEQMGKNLPKPKAIVCISAHWETKGTKVTANPHPETIHDFGGFPQ
                     ALFDAQYPAPGDPELALELGRMVHSAGVQQDLQWGLDHGAWSVLKPMFPLADIPVIQL
                     SLDYTKTPQQHFEFAKELAALRDRGVLVMGSGNIVHNLGRIDWQHPNSGYDWAIEFND
                     QVKKTILDRNFAQLSNYKSLSKSAELAVPSTEHYLPLLYATALATEKDQLEFFNDDPQ
                     MGSLTMTSLKIS"
     gene            4155..4580
                     /gene="ruvX"
                     /locus_tag="PK281_07460"
     CDS             4155..4580
                     /gene="ruvX"
                     /locus_tag="PK281_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HOY49221.1"
                     /translation="MPRILAIDFGTKRIGIAVTDPLQMIANPFKTVTNEEALPFLKTY
                     LSKESVETIVIGEPKHLDGSASGPQESITNFVRAVEKAFPTVKIVRVDERFTSKLAFG
                     AMIDGGASKKQRQDKSLIDTLSAVIILQSYMEQKAFGKM"
     gene            4590..5153
                     /gene="def"
                     /locus_tag="PK281_07465"
     CDS             4590..5153
                     /gene="def"
                     /locus_tag="PK281_07465"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013186992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOY49222.1"
                     /translation="MILPIVAYGTPVLKQKGAFIDSEHPGLKELIANMFETMYHAPGV
                     GLAAPQVGHSIRLFVLDASPFSDEHPELDGVKKVFINAEIVEESGDDWKFNEGCLSIP
                     HIREDVDRKPTIRMKYQDENFNSFEETFDGILARIIQHEYDHIDGILFTDRLSQLRRT
                     LLRGKLQDISKGRVKVDYRMKFTQLRR"
     gene            5200..5775
                     /locus_tag="PK281_07470"
     CDS             5200..5775
                     /locus_tag="PK281_07470"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOY49223.1"
                     /translation="MKLFKLFTVLSFSALILITACGGGRDEKLAKIKDLETRLMQDSL
                     KPKDEVAAYNLQVAYTEFAELYPKDSLAPVYLYKAANLSMNLNWGKSAIDILEKFLTL
                     YPDHKLASDALFFKGFVFDNQLNDDAKAGECYREFLKKYPTHAFASSAEASIHNLGKT
                     DEELIREFEKMNSDTSKQAATTETAAVPATK"
     gene            complement(5776..6798)
                     /gene="tsaD"
                     /locus_tag="PK281_07475"
     CDS             complement(5776..6798)
                     /gene="tsaD"
                     /locus_tag="PK281_07475"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013551840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOY49224.1"
                     /translation="MERQPVYILGIESSCDDTAAAVLADDKMLANVVAGQEVHRNWGG
                     VVPELASRAHQQHIVPVVDEALRQAKISKHELHAIAYTRGPGLLGSLLVGGSFAKGLS
                     FGLNIPLIEVNHMQAHILAHFIAGDKPAPSFPFLCLTVSGGHTQIVRVDNPLSMHIIG
                     ETRDDAAGEAFDKVAKILGLPYPGGPMVDQLAKTGNPHRFIFPEPKAEAFDFSFSGLK
                     TSFLYLVRDELKKDPSFLEKNMQDLCASVQFTIVKILTGKLLKAAQATGIRTLALAGG
                     VSANGGLREAMLALENNGYNIHIPPMGFCTDNAAMIAMTGYFKYKQGIFSGTEVGSLA
                     RYPLGS"
BASE COUNT         2095 a         1397 c         1475 g         2064 t
ORIGIN      
        1 gttagaagct tcatttatct atgaagatac gcccgatcaa gtaaaagcta cgcgtgacgt
       61 gaagcgcgac atggaaaaag atgctccaat ggatcgtctt gtgtgtggtg atgtgggttt
      121 tggtaagact gaaattgccg tgcgtgcagc attcaaagcg gtaaatgata gtaagcaagt
      181 ggctattctc gttcctacta ctattcttgc gcttcaacat tataaaacct ttgctgaacg
      241 tttgtcacaa cttccgtgta cagtcgatta catcaaccgt ttcaaatcac ctaaagagca
      301 aaaagaaaca ctcgaacgat tagctaaagg cgaaatcgat atcctcattg gaacgcatcg
      361 tatcatcagt aaggacgtta aattcaagga tttaggtttg atgattatcg acgaagagca
      421 gaaatttggc gtggcagcta aagagaaact caagcagatc aaagtcaatg ttgatacgct
      481 tactttgact gctacaccaa tcccgcgaac cttgcatttt agtatgatgg gcgctcgcga
      541 tttgagtgta atcaatactc cgccaccgaa tcgtcacccc gttcaaaccg aattacatac
      601 gtttaacgaa gagctaattc gtgatgctgt aacatttgaa gtacaacgtg gcggacaggt
      661 tttcttcgtt cacaatcgag ttcaaaacat tcaagaagtg ggcgatatga ttcgtcgact
      721 ttgtccggga gtgcgtgtgt gttgcggtca cggacaaatg gagccagagg agttggaaaa
      781 tgtgatgatg cgctttatcg acggtgatta cgatgtgttg gtagcaacga caatcataga
      841 atcgggattg gacatttcga atgccaatac gattctgatt aataacgcac acttatttgg
      901 attgagcgat ttgcatcaaa tgcgcggtcg ggtagggcgg agtaacaaaa aagcattttg
      961 ctatttgctt accacgccag tatcgttgct cacaagtgat gctcggaagc gattgaaagc
     1021 cattgaggat tttgctgaat taggtagcgg tttcaacata gcaatgcgcg atttggatat
     1081 tcgtggtgcg ggcgatttat tgggaggcga acaaagtggt tttatctcgg agattggatt
     1141 tgagatgtat aataaaatcc tcgacgaagc tattcaggag ttgaaggaga gtgatttcaa
     1201 gcaattgttt catgaagagt tagaggagaa acgtaaagtc tgggtgagcg attgtcagat
     1261 tgataccgat ttagaaattc tcataccgga tgcttacgtg accaatatta ccgagcgttt
     1321 gcttttatac aaggacctcg ataacatgga aaccgaggtt gaattacagc aattcgaaaa
     1381 gcagctaacc gatcgttttg gcgcagttcc gaagccaaca ttggagttaa tgaatgccat
     1441 ccgtttacgt tggattgcta aggacatggg ctttgagaag gtcgtattaa aacagaaaac
     1501 cttcgtagga tatttcgttc aaaaccaaca aagtagcttc taccaaagcg atttctttgc
     1561 tcgtttgatg cagcatttaa ctcgaattcc cgggaaagct cgtttgaaag aacgaaacaa
     1621 caaactcacc ttggtgatgg agcaaactgg taatctggat caagccgtaa atgctctcgg
     1681 acaattatta gtagctactc gtcctcaaga acaaacactc aactgatgca cgttttctat
     1741 acgactactt ttcagggcga taaggcgatt ctgaccgaag aagaaagtct gcacctaagc
     1801 aaagttttac gtcttaaaga aggtgcttct gtattattgt tagatggaaa aggaggaatg
     1861 tttgaagggg aagttttatt ggttcataag aagaactctg caattgggaa tttgcgtcgt
     1921 ttacctgtta ccaatagtcg tccataccgc ttgcatgttg ctattgctcc aactaaaatg
     1981 atggaacgac ttgagtggtt tctcgaaaaa gctactgaaa ttggaatcga tgaaattact
     2041 cctttaatca ccaatcggac agagcgtgat caagtgaaaa tgcaacgggt agagaaaatc
     2101 gttcttgctg ctatgaaaca aagtatgaca gctaccatgc ctgttttaaa tgaggttacg
     2161 acttttgcga cattcttaaa gaaagaaaca tccggtttga aactcatcgc acattgcatt
     2221 ccgggtgaaa aacctttgct aactcaaaaa gctagtcaag cagatcaaat caccattctt
     2281 gttggtcctg aaggcgattt cactgccgag gaagttggat cagctttgaa ggttggatat
     2341 gaagcaattt cccttgggca aagtcgtttg agagcagaaa cagctggagt tgttgtttgt
     2401 gctcaagtac aagcatcttg gcagattaaa gcttagatta aatcacgaca tttgtccgta
     2461 attatttttc gatgaaacgt tttctgattc ttgttctaac tgttttaagt gtcacctcga
     2521 tacaggcgca aggcacgtac agcatcggtt tgctcaaata caacggtggt ggagattggt
     2581 atgctaatcc aacatcactt actaacctga tttcattctg caataaaaat atcggaacca
     2641 acatttctcc cgatgcgatt acagttgatg caggaaatcc agaaatattc aacttgccat
     2701 ttgtccacat gaccggacat ggtaacgtca ttttcagtcc ttctgaaagc gacaaccttc
     2761 gtacgtattt aattgcagga ggatttttac atgtggatga taattatgga atggatccgt
     2821 tcattcgtcc acagttgaag cgaatttttc cagaattgga tttggttgag ttaccaccat
     2881 cgcatccaat ttttcaccag aaattcgatt ttccaggcgg tttgcctaag attcacgaac
     2941 acgatggtac agcgccacag gcatttggaa tgttctggga aggtcgctta gttgtcttat
     3001 atacttttga gtgcgatttg ggcgatggtt gggaagatgc ttctgttcac aaggattcag
     3061 aaagtgcccg tcagaaagct ttaaaaatgg gagccaatat gctgcaatat gtattaatgg
     3121 ctaatgatcc aatcaaacgc caataatatt ctgctgtact agttattaca aaacttagtt
     3181 ctcaattttt cagagatctt ctgaatcacg aaatcttcaa gctagtcata gttaacgagc
     3241 ccatttgtgg atcatcgttg aagaattcta actgatcttt ttcggtagct aaagcggttg
     3301 cataaagtaa aggcaagtaa tgttcggtcg aaggaactgc taactcagcc gatttactca
     3361 aacttttata gttactcaat tgagcaaaat tgcgatctaa aatggttttc ttcacttgat
     3421 cgttaaattc aatggcccaa tcgtatccgc tattgggatg ttgccaatcg atacgaccta
     3481 aattgtggac aatattgcca ctgcccatga ctaaaacacc acgatctctt agagcggcta
     3541 attctttggc aaattcaaaa tgttgctgag gagttttggt ataatctaaa ctcaattgta
     3601 taactggaat atcagctaag ggaaacatgg gtttaagcac actccaagct ccatgatcta
     3661 atccccattg caaatcttgt tgcacgccag cactatgcac cattcgtcct aattctaaag
     3721 ctagttctgg atcgccgggt gccggatatt gtgcatcaaa taaagcttgc ggaaatccac
     3781 caaaatcatg aatcgtttcg gggtgtggat tggcggtaac cttcgtgcct tttgtttccc
     3841 aatgtgccga aatacacacg attgctttgg gttttggtaa attcttgccc atctgctccc
     3901 aagccaaaga gtatttattc ttttctatgg cattcatcgg atttccatgt ccgacaaata
     3961 aaactggcat gcgttccgag ctttttggtc cggatgccaa ttcacgtaaa ctcactaaac
     4021 tagctcccat aaggcttatc gaggtggcgt aaagaaactt tctgcgcttc atcctacaaa
     4081 gataaaataa atgtacatac atacattttt gaatcattaa aatgtttcac ctttaccgct
     4141 aaagaagaat ccctatgccc cgaatattag ccattgattt tggaacgaaa cgcattggaa
     4201 tagcagtgac cgatcctttg caaatgattg ctaatccgtt caaaacggtt acgaatgaag
     4261 aggcattacc attcttgaaa acctatcttt caaaggaaag cgtagaaacc attgtgatag
     4321 gggaacctaa gcatttggat ggttctgcta gcggtccgca agaatcaatt accaactttg
     4381 ttagagcggt tgaaaaagca tttccaactg taaagattgt tcgagtagat gaacgattta
     4441 caagtaagtt agcatttggt gctatgatcg atggtggagc gagtaaaaag cagcgtcagg
     4501 acaaatcttt aatcgatacc ttgagtgctg tgattatttt acaaagctac atggagcaaa
     4561 aagcatttgg aaaaatgtaa ttttgccgca tgattttacc tatcgtagca tacggaacgc
     4621 ccgttttaaa acaaaaagga gcttttattg attccgaaca tcctggattg aaagagctga
     4681 tcgccaacat gttcgaaact atgtatcatg cacccggagt gggtttagca gctccacaag
     4741 tgggacattc gattcgacta tttgtgttag atgcatcacc tttttctgat gaacatcccg
     4801 aattagatgg tgtgaaaaaa gtatttatca atgccgaaat agtggaggaa tcgggtgatg
     4861 attggaaatt taatgaaggt tgtttgagca ttccacacat tcgtgaagat gtagatcgga
     4921 aaccaacgat aagaatgaag tatcaagacg aaaatttcaa ttcgtttgaa gaaacattcg
     4981 atggaatttt agcgagaatc attcaacacg aatatgatca tattgatggt attttattta
     5041 ctgatcgcct gagtcagctt agaagaacgc tacttcgcgg taagctacaa gatatttcaa
     5101 agggtagggt gaaagtagat tatcgaatga aatttactca gcttcgtagg tagtgttcag
     5161 ctttttctat ttttacgctc cataacccag aatatccaca tgaaattatt caagcttttt
     5221 acagtactat cattttctgc attaatctta attactgcct gtggtggtgg acgagatgag
     5281 aaattagcca aaattaaaga tctcgaaact cgtttgatgc aggattctct taagcctaaa
     5341 gatgaagttg cagcttacaa tctgcaagtt gcttatactg aatttgccga gttgtatcct
     5401 aaagattcgc tggctcctgt atacttatac aaagcagcaa atcttagcat gaatctcaat
     5461 tgggggaaat ctgccataga tattctagaa aaattcttaa ccctatatcc tgatcataaa
     5521 ttggcttccg atgcactttt ctttaaagga tttgtgttcg ataaccaatt gaatgatgat
     5581 gctaaagctg gtgaatgtta tcgtgagttc ctcaaaaaat atcctaccca tgctttcgct
     5641 tcaagcgcag aggcatccat tcataacctt ggaaaaacag atgaagaatt aattcgtgaa
     5701 tttgaaaaaa tgaattccga tacttctaaa caagctgcaa ctacagaaac ggcagctgtt
     5761 cctgcaacca aataattagc ttcctaaagg atatcttgca agtgaaccaa cttcggttcc
     5821 tgaaaatatt ccttgcttgt atttgaaata acccgtcatg gcaatcatgg ccgcattgtc
     5881 ggtgcagaag cccattggtg gtatatggat gttgtatcca ttattttcta aagccagcat
     5941 cgcctctcgt aatccaccat tagccgaaac tcctcctgct aaagctaatg tgcgaatacc
     6001 tgttgcttgc gctgccttta gtagctttcc tgtcaaaatt tttacaatag taaattgaac
     6061 agaagcacat aaatcctgca tgttcttttc taaaaagcta gggtctttct tcaactcatc
     6121 tcgcaccaaa tacaagaatg aagtcttcaa tccactaaaa ctaaaatcaa atgcttcggc
     6181 tttcggttct ggaaaaataa atcgatgtgg attacctgtt ttagctaatt gatctaccat
     6241 agggccaccc ggataaggaa gtcctaaaat cttcgctact ttatcaaatg cctctccggc
     6301 agcatcatcg cgggtttcgc ctataatatg catgcttaaa ggattatcta cccgtacaat
     6361 ttgtgtatgt cctcccgaaa cggtcaaaca caaaaatgga aaactcggtg caggtttgtc
     6421 tccagctata aaatgagcta gaatgtgcgc ctgcatgtga tttacttcta tcaatggtat
     6481 gttaagtcca aacgaaagtc ctttcgcaaa cgaacctcca accaataaac tacctaataa
     6541 tccgggtcct cgcgtataag ctatcgcatg tagttcatgt ttgcttattt ttgcctgacg
     6601 aagcgcctca tccacgaccg gaacgatgtg ctgttgatgt gccctagacg ctaattctgg
     6661 aacaactccg ccccaattgc ggtgaacttc ttgtccggca accacattgg caagcatttt
     6721 gtcatccgcg agcacggctg cagctgtatc gtcgcatgaa gattcaatgc ctaatatgta
     6781 caccggttga cgttccatca atttctttct tattaaacta ctccgcaaaa taacaggatt
     6841 tttcttgctt tcggtactgc ttctgctgac cggatgcctt attctgttcc aaagtggacg
     6901 ttttcaaacg tggttagctc aagaagttgc cgcttgaatc agcgaatcga ttggtgctaa
     6961 agtcgaaatc aaatccataa aaataaattt cttcgatcaa agttgaattt catttctctg
     7021 tccgttcttc g
//
LOCUS       DAOOQW010000117         7003 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_13216_length_7003_cov_4.975101, whole genome shotgun sequence.
ACCESSION   DAOOQW010000117 DAOOQW010000000
VERSION     DAOOQW010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 7003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7003
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13216_length_7003_cov_4.975101"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            172..1851
                     /locus_tag="PK281_07480"
     CDS             172..1851
                     /locus_tag="PK281_07480"
                     /inference="COORDINATES: protein motif:HMM:NF016274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004668 - protein-arginine deiminase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HOY49225.1"
                     /translation="MKNFNPLLFIFLFLFSFQVKAQTISNTPPAGTIRTAAEWEEIQA
                     LVVTWTDYKSVLAEIIRYAQEECKVIVHCTNANTAQNELVSTYGVPVGPNVIFKVQPY
                     NTLWIRDYGANTAYINDVDSLFLVDWKYNRPSRTKDDTIPRGYARLLNINLVQTTTSP
                     NQVIHTGGNFMSDGLGTAFSSELVLDENPTQSETAINNIMLNYMGIDRYIKMATLPYD
                     GIHHIDMHMKLLDEETLLIGQYPTGISDGPQIEANLLYVIDNFNSVFETPYKIIRIVQ
                     PPDDLNKYPSQNGDYRTYSNAVFVNKTVLLPIYEEKYDTTALRIWRESLPGFRVIGID
                     CNSIIPASGAIHCITHSVGVNDPMLIVHQPLHDTYDTANDYLVSASLQHRSGINQAKL
                     FYTIDTTQAYSEVPMTLFNSATNQWTATIPAQAAGTEVFYFIQGIAESGKTQFRPISA
                     PEGYWSFNVLDSGSVITFQQEIMDKLFEVEAYPNPSKGITCIPVRSDVAMNVDLSVLD
                     VSGRMVEQIAISKISSGESRFYINTTNWSAGMYFIRAQTPYGSRMQKLMVR"
     gene            1988..2347
                     /gene="rpsF"
                     /locus_tag="PK281_07485"
     CDS             1988..2347
                     /gene="rpsF"
                     /locus_tag="PK281_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019388304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOY49226.1"
                     /translation="MLKQYETVFIMTPVLSDDQMKEAVGKFKAMLADNGAKLVHEENW
                     GLRKLAYPIQKKTTGFYYLLEYQAPGEMISKLEVAFKRDERMLRFLTVALDKHSLAYN
                     EKKRTKGFAKSAVKTEA"
     gene            2370..2639
                     /gene="rpsR"
                     /locus_tag="PK281_07490"
     CDS             2370..2639
                     /gene="rpsR"
                     /locus_tag="PK281_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HOY49227.1"
                     /translation="MASPQSEIRYLTPPTVEVKKKKYCRFKKSGIKYIDYKDADFLLK
                     FVNEQGKILPRRITGTSLKYQRKVSQAIKRARHLSLMPYVADMLK"
     gene            2649..3095
                     /gene="rplI"
                     /locus_tag="PK281_07495"
     CDS             2649..3095
                     /gene="rplI"
                     /locus_tag="PK281_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOY49228.1"
                     /translation="MEIILTQDVNNLGHKDEIVKVKNGYGRNYLIPQGMGQLATESNR
                     KVLAETLRQRAHKEEKIRTEATTMAEKLANTKLEVGAKVGESGKIFGSVNALQIAEAL
                     KKLGMDIDRKQISIDSENIKQLGTYSAGVKLYKDIKATVEFEVVAE"
     gene            3265..3486
                     /locus_tag="PK281_07500"
     CDS             3265..3486
                     /locus_tag="PK281_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019039083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49229.1"
                     /translation="MKTYQIDVLNPKAVKLLQDLADLNLISIRKTDENLSIVLKRLRK
                     KASINTPSIEEITQEVELVREKRNASKKR"
     gene            3467..3880
                     /locus_tag="PK281_07505"
     CDS             3467..3880
                     /locus_tag="PK281_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018943504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HOY49230.1"
                     /translation="MLPKKDRIVIDTNLWISFLLSGNYAKLDKLIYTNSVVLLFSDEL
                     LEEFIDVACRPKFRKYFTTEDLIKLLFIIEQNAEFIQVESELAICRDPKDNFLLALAL
                     EGKATHLLTGDKDLTELEKIGGTVIIPLTEYLKNK"
     gene            complement(3906..4886)
                     /locus_tag="PK281_07510"
     CDS             complement(3906..4886)
                     /locus_tag="PK281_07510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HOY49231.1"
                     /translation="MTKRIIFFLVLFTLPLLYFIPHASSWGFFAHKEINRVAVFTLPP
                     ELFGFYKQNIEFITEHAVDPDKRRYAVKDEAARHFIDLDRYGASPLDSMPHNWEAACK
                     KYTEDTLRAHGIVPWHIDVMVKRLTAAFKSGDYDRILRTSADLGHYIGDAHVPLHTTR
                     NYNGQLTNQKGIHGFWESRLPELFNEDYDFFIGKAKYVENPLELAWKIVGESFAAKDS
                     VLRFEAELNERFPTDRKYSFENRGASMMKVYSEDYSAEYNKMLDGMVERRMRQAILTV
                     GSLWFTAWVNAGQPDLNVYKNSKLGSDSERELAEEEQNYQKGAIKGRSCD"
     gene            complement(4946..5677)
                     /locus_tag="PK281_07515"
     CDS             complement(4946..5677)
                     /locus_tag="PK281_07515"
                     /inference="COORDINATES: protein motif:HMM:NF015962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HOY49232.1"
                     /translation="MSLTVQTCFSPALYPLFANKEAIIIVVDVLRATSAICTALHHGV
                     ERMIPVAGLDEARKYKDLGYLVAAERNAVKQEGFDFGNSPFHYMDEQVKGKTIVISTT
                     NGTQAIEAAKDASVIAIGSFLNLSALAEWILPQERDVIILCAGWKNKFNLEDSLFAGA
                     LANKLIDSGNFSTMCDSTIAAGYLYERASDDLYGFLEYSSHRKRLKNLHLEDDIRYCL
                     STDIMQTIPVYQDGGLVSAQKIVVL"
     gene            complement(5696..6799)
                     /gene="gcvT"
                     /locus_tag="PK281_07520"
     CDS             complement(5696..6799)
                     /gene="gcvT"
                     /locus_tag="PK281_07520"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010136770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HOY49233.1"
                     /translation="METTPAVKRTPFYSIHEQLGAKILPFAGYEMPITYAGVIAEHEC
                     VRNAVGVFDVSHMGEFILRGEHALDLIQLVTSNDATKLVDGKVQYSCFPNDRGGIVDD
                     LLVYRIKEDQYMLVVNASNMEKDWNWLKKQDRWGVDMEDISDRTALLAVQGPKAAATL
                     QKLTDLDLSAMEYYTFAKGTLAGFSNVLISATGYTGAGGFEIYFDEKNAEEIWNAVFE
                     AGAEFDIQPVGLACRDTLRLEMGFCLYGNDINDSTSPIEAGLGWITKFTKEFINRSEI
                     EHQKQVGVQKKLVGFELIDKGVPRHDYEICSDSGDVIGIVTSGTMSPSLKKPIGMGYV
                     PTSYAPIGSVIYIRVREKLLKAKVVKMPFLTNQ"
BASE COUNT         2215 a         1415 c         1286 g         2087 t
ORIGIN      
        1 caaattactt ttatctctta aggaataacc aaagccggct ctccgtagta aatgggcagt
       61 tttctgtgct tctgtcataa aatgagaatt tgctattcaa gagtctaaat gtcagtatcg
      121 gtttaatcta cctttatgta tcttcgtttc cttccaattg gatgttagta tatgaagaac
      181 ttcaacccat tactttttat tttccttttt cttttttctt ttcaagttaa agctcaaact
      241 attagcaata ctccaccagc tggaactatc cgcacagctg ccgaatggga agaaattcaa
      301 gcattagttg ttacctggac tgactacaaa tctgttttag cagaaattat tcggtatgcc
      361 caagaggagt gcaaagtaat tgttcattgc accaatgcca atacagctca aaacgaattg
      421 gtttcaactt acggcgttcc ggttggaccg aatgtaattt tcaaagttca accctacaat
      481 acactttgga ttcgtgatta tggcgcaaat acagcctata tcaacgatgt agattcactg
      541 tttctagttg actggaaata caaccgtcca agtcgtacta aagatgatac gattcctcgc
      601 ggttatgctc gtttgttgaa tattaattta gttcaaacta ccacttcgcc caatcaagtt
      661 attcatacag gtggaaattt catgagtgat ggattaggaa ctgctttttc ttctgaatta
      721 gttttagatg aaaatcctac gcaatccgaa acggcaataa acaacatcat gcttaattac
      781 atgggcattg atcgttacat caaaatggca accttgccct acgatggtat tcatcacatc
      841 gatatgcaca tgaaactgct cgatgaagaa acgttgctca ttggtcaata tccaacaggc
      901 atttctgatg ggcctcaaat cgaagcgaat ctgctttatg tgatagataa tttcaattcg
      961 gtatttgaaa caccttataa gattattcgc attgttcaac cacccgacga tttaaacaaa
     1021 tacccaagtc agaacggtga ttatcgtacg tattctaatg ctgtattcgt caataaaacg
     1081 gtgcttttgc caatttacga agagaaatac gacaccactg ctttgcgaat ttggagagaa
     1141 agtttgcctg gttttcgggt gattggaatt gattgtaact cgattattcc tgcaagtgga
     1201 gcaattcatt gtattacaca ttcggttggt gtaaatgatc cgatgttaat tgtgcatcag
     1261 ccattgcacg atacgtatga tactgcgaac gattatttag ttagtgctag tttgcaacac
     1321 cgcagtggaa tcaatcaagc taaactgttt tatacaatcg acacgacaca agcgtattcc
     1381 gaagtgccca tgactttatt caacagcgct acaaatcaat ggactgctac aattccggca
     1441 caagcagctg gaacagaagt cttttatttt atccaaggaa ttgccgaaag tgggaaaact
     1501 cagtttcgtc cgattagcgc acctgaagga tattggagtt ttaacgtact cgattcaggt
     1561 tcagtgatta cgtttcaaca ggaaataatg gataagcttt ttgaagtaga agcttatccg
     1621 aatccttcca aaggcattac ctgcattcct gttcgcagcg atgtggcgat gaatgtcgat
     1681 ttgagcgtat tagatgtttc gggaagaatg gtggaacaaa ttgccatttc taagatttca
     1741 tcaggtgaat ctcggttcta tatcaatacg actaattggt cggctggcat gtattttatt
     1801 cgtgctcaaa cgccttatgg tagccgcatg cagaaattga tggtgcgtta atttactgga
     1861 aaggttggat gatgtatgat tcactaaatg aagtgatcaa ttaatattta agactcatcc
     1921 gaatttttag gttgtgtaat cgattagatt cattactttt gccgtccaat ttttaaacat
     1981 tgtgcccatg ctaaaacagt atgaaaccgt tttcattatg actcccgttt tgtctgatga
     2041 tcagatgaag gaagcggtcg gcaagtttaa agccatgctt gcagataacg gcgcgaaact
     2101 agttcacgaa gagaactggg gactgcgcaa actggcttac cctattcaaa agaagacaac
     2161 aggcttctat tatttactcg agtatcaggc tccaggtgag atgatttcta aactcgaagt
     2221 agcattcaaa cgtgatgagc gtatgttgcg attcttaacc gttgctctcg acaagcattc
     2281 acttgcttac aacgagaaaa aacgtactaa aggatttgca aaatccgcag ttaaaactga
     2341 agcctaacca ctaaaaaaac ttttgaacga tggcaagtcc acaatcagaa atccgctatc
     2401 tgaccccgcc aacggtggag gtgaagaaga aaaagtactg ccgctttaag aaaagtggta
     2461 ttaagtacat cgattacaaa gacgcagact tccttttgaa gttcgtgaat gagcaaggaa
     2521 aaattcttcc acgccgtatc acaggtactt ctttgaagta tcagcgtaaa gtttctcagg
     2581 cgatcaaacg tgcacgtcac ttatcgctta tgccttatgt agctgacatg ttgaaataag
     2641 aaggaatcat ggaaatcatt ctcacacaag acgtaaataa ccttggtcac aaggacgaaa
     2701 tcgtgaaggt aaaaaacggt tatggccgta actacctgat tccacaaggt atggggcaac
     2761 tcgctaccga gagcaaccgt aaggtattag ctgaaacact tcgtcaacgc gcgcataaag
     2821 aagagaaaat ccgtacagaa gcaactacca tggctgagaa attagctaat actaaattgg
     2881 aagttggagc taaagtaggt gaatctggaa aaatctttgg ttctgttaat gcattgcaaa
     2941 ttgctgaagc attaaagaaa cttggaatgg atatcgaccg caagcaaatc tctatcgaca
     3001 gcgaaaacat caaacaatta ggaacatact cagctggtgt taaattatac aaagacatca
     3061 aagcaacagt agaattcgaa gtggttgctg aataagctat ctcatctcat acaaaagccc
     3121 tgtaacttaa ttgctacagg gcttttttgt tgggcgaata aaattttctt gagtcaattg
     3181 ttttatctag caaacacaat tatttgagtt ctcgtagcat tgaaggttat gaatacgata
     3241 aaataaaact gttacatttg aaatatgaaa acatatcaga tagatgtttt aaatcctaaa
     3301 gcagttaaac tacttcaaga tttagctgat ttgaacttaa tttccattcg aaaaacggat
     3361 gaaaatctta gtattgtttt aaaaagattg agaaaaaagg cctcaatcaa tacaccttca
     3421 atcgaagaaa ttacccagga agttgaatta gttcgagaga aacgaaatgc ttccaaaaaa
     3481 agataggatt gtaattgata ctaacctttg gattagtttt cttctgtctg gaaattacgc
     3541 taagcttgat aagttaatct atacaaattc agttgtacta ttattcagcg acgaactttt
     3601 agaagagttc attgatgttg catgtcgtcc gaagtttagg aaatacttta caactgaaga
     3661 tcttataaaa ctacttttta taatcgaaca gaatgctgaa tttattcaag tcgaaagtga
     3721 attagctatt tgtagagatc ctaaagataa ttttcttctt gctttagctc tagaagggaa
     3781 agcaacacac ttacttacag gagacaaaga tttaaccgaa ttggaaaaaa ttggaggaac
     3841 tgtaattatt ccactaacag agtatttaaa gaataaataa ctaggtagaa tattattatt
     3901 tatactcaat cacagcttct tcctttgatt gctccttttt ggtaattctg ctcttcttct
     3961 gctaattcac gttcagaatc agaacctaat ttgctattct tatatacatt caagtcaggc
     4021 tgtcctgcat ttacccaagc ggtaaaccat aaacttccaa ccgtcaagat tgcctgacgc
     4081 atacgtcttt caaccatgcc atccagcatt ttattgtatt cagccgaata gtcttcgcta
     4141 taaactttca tcatcgaagc gccccggttt tcaaaactgt atttacgatc cgttggaaaa
     4201 cgctcattta actccgcctc aaatcgtaat accgaatctt ttgcagcaaa actttctcct
     4261 acaatcttcc aagctaactc caaaggattt tccacatact ttgctttgcc tataaagaag
     4321 tcgtagtctt cattgaacaa ttcaggtaaa cgcgattccc agaaaccatg aatacctttt
     4381 tgattcgtta attgtccgtt gtaattacgt gttgtatgca atggaacatg cgcatcacca
     4441 atataatgtc ctaaatcggc tgaggtgcgt aaaatacgat cgtaatcgcc cgacttaaaa
     4501 gcagcagtta agcgtttcac cattacatca atgtgccaag gaacgattcc atgtgcgcgc
     4561 aaggtgtctt cggtgtactt tttacaagct gcttcccaat tatgaggcat cgaatcgaga
     4621 ggtgaagcac cataacggtc taaatcgata aagtggcgag ctgcttcatc tttaacagca
     4681 tagcgacgtt tatccggatc gacagcatgt tctgtgataa attcaatgtt ctgtttatag
     4741 aaaccaaaaa gttctggagg aagcgtaaat acagctactc ggttaatttc cttatgcgca
     4801 aaaaatcccc aactacttgc atgtggtatg aaatacaaca agggtagagt aaataatact
     4861 aagaagaaga ttatgcgctt tgtcatcttt caaagataag cgttcgaagc atttactgtt
     4921 gcttcagata aagagaaatt gtctgctata aaacgacaat cttttgagct gaaactaatc
     4981 caccatcctg atatacagga atcgtttgca ttatatcggt acttaaacaa taacgtatat
     5041 cgtcctcaag atgcaagttt ttcaggcgtt tccgatggga tgaatactct agaaatccat
     5101 ataaatcatc cgatgcacgc tcatataagt aaccagctgc aatggtcgaa tcgcacatcg
     5161 tactgaaatt acccgaatca attagcttat tagctaatgc gccagcaaac aacgaatctt
     5221 cgaggttaaa tttatttttc caaccggcac aaaggataat cacatcgcgc tcttgtggaa
     5281 gaatccattc ggctaatgca ctcagattta aaaacgaacc gatagcaatc acagatgcat
     5341 ctttagccgc ttcaatcgct tgagttccat tggtggttga aatgacgatc gtttttcctt
     5401 tcacctgctc atccatgtaa tgaaaaggcg aattcccaaa atcaaaacct tcttgtttaa
     5461 cggcattacg ttcagcagca accaaataac ctaagtcttt gtatttgcgt gcttcatcta
     5521 atccagcaac aggaatcatc cgctccacac catgatgcaa ggcagtacaa atggccgagg
     5581 ttgcccgaag cacatctact acaatgatta ttgcctcttt attcgcaaat aaagggtaca
     5641 atgcgggtga aaaacaggtt tggacagtta gtgacaagcg ctagaactta aaagattact
     5701 gattagtcag aaaaggcatt tttaccacct tagctttaag cagcttttcc cgaacacgaa
     5761 tatagataac agatccaatt ggagcataac ttgttggaac ataacccata cctattggtt
     5821 ttttcaagct aggcgacatt gttcctgaag taacaattcc aattacatca ccactatcgg
     5881 agcaaatctc ataatcatga cgaggaactc ctttatcgat taactcaaaa ccaactaatt
     5941 tcttttgtac gccaacttgt ttttgatgtt caatttctga gcgattgatg aattctttgg
     6001 tgaatttggt aatccaacct aaaccagctt caatagggga agtactatcg ttgatatcat
     6061 tgccatacaa gcaaaatccc atttctaaac gtaaagtgtc gcgacaagct aaaccaacag
     6121 gttgaatgtc gaattcggca cccgcttcaa aaacggcatt ccaaatttct tccgcatttt
     6181 tctcatcaaa ataaatctca aaacctccag caccagtata cccagttgct gaaatcaaca
     6241 cattcgaaaa tccagcaaga gttcctttag caaaggtgta atattccatt gctgaaagat
     6301 ctaaatcagt cagtttttgc aatgtagccg cagctttcgg cccttgaacg gcgagtaggg
     6361 cagtacgatc ggaaatatcc tccatatcaa ctccccagcg atcttgcttc ttaagccaat
     6421 tccaatcttt ctccatgtta gaagcgttaa caactaacat gtattggtct tccttaatgc
     6481 ggtataccaa aagatcgtct acaattccac ctcgatcatt gggaaaacac gaatactgta
     6541 cttttccatc cacaagttta gtagcatcat tcgaggtaac caattgaatt aaatccaatg
     6601 catgttcacc acgaaggata aattcaccca tgtgactcac atcaaaaaca cccactgcat
     6661 ttcgaacaca ttcatgttca gctataactc cagcataagt gataggcatt tcatagcctg
     6721 caaaaggtaa tatcttagct cctaattgtt catgaatcga ataaaaggga gtgcgtttta
     6781 ctgctggagt ggtttccatg gttgtgtgtt tttctggctg caaacgtagc aaatcctgat
     6841 gtttttttca cggtattcta tcaagccatt ccacgaacct gattttggta tattcgcagg
     6901 gattttttaa ttcttaatcc attcggaaac ttcctcctta tgaaaattaa attgctttta
     6961 agcgttttct ccattctatt gaatctagga ctattcgctc aat
//
LOCUS       DAOOQW010000118         7002 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_13218_length_7002_cov_4.517202, whole genome shotgun sequence.
ACCESSION   DAOOQW010000118 DAOOQW010000000
VERSION     DAOOQW010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 7002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7002
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13218_length_7002_cov_4.517202"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(235..2445)
                     /locus_tag="PK281_07525"
     CDS             complement(235..2445)
                     /locus_tag="PK281_07525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOY49234.1"
                     /translation="MKTQRLQILIVEDNTGDFLLIQQMLHEIRDFSKDIIHVTSLQDA
                     IHSVQTSEPDVILLDLSLPDSYGFDSFQRLNGISPAIPILILSGLNDTRFAHEAVKNG
                     AQDYLVKGEFEEKLLAKSILYSIERKLSMELIRQSQASYKLLFENNPIPMYIREKDGL
                     RILKVNQSAIDHFGYSEEEFLKMQVSDLHPPEEVETLKRMIRERYGDYSTAEVFHHVC
                     KDGKIVTVECRVQETELDGEVCYLVLADDVTEKRHVQEEMLFQSGVLKNVRDTIFVTD
                     RKGIITYWNEGAELSFGYSRDEIVGRNFDVLYAEVDKLNVRAEQHEILAGRITQWETR
                     LITKDDKIVWSDIKASHLYDENGMIVGIIRVCKDITQSRYFSEKQKETVAMLNSIFNN
                     VNQSIVLLDQSQRIKAFNVIANRQNIQLMGQELKENIPFSDYLLADVQDSFRAKFEES
                     MLDKQVQWEMAYRFSATSVHWYSISLSPVSDEKQNVLGACVSMMNITERKMADEKFRG
                     QFLEIENNNKELDRLVKILSHDLKAPMNSVSGLITLAREEKNPEEFGNYLDMMEKSLK
                     KLETFTNDIIASLRSRGQVVAVKVDLFEQVQDVMDELRFVPGAETIRFVNEIGPNVNL
                     NSDPARLRVILSNLISNAIKYSDPSKLDCFVKVTCKEYPLLLEIEVEDNGIGIAAEHH
                     KRIFDSYYKLSDRSDSNGLGLSNVKDAVQKLQGTIEVESTLGLGSIFKIQLPAI"
     gene            2741..5266
                     /locus_tag="PK281_07530"
     CDS             2741..5266
                     /locus_tag="PK281_07530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOY49235.1"
                     /translation="MITLAINLYQQLFDQGIIPRCLVTLDGDFVLTNSAFRKMFQLTG
                     THMELKNFNLFDVLSDATGIHKERIRKEQLFYSDTDEAIEGKIIILNNDGSKTKLDYL
                     RSRVEMENLSMLSFEWQDVTEKVEYRKSLRSTYERLQLAASISNLGVWEYEFETKEWY
                     LNEWMCALFTIPAHGKTDITQSIVASVIPEDLELLSQKWIAAKEKGDHLDFEFRIVLP
                     DSGVHYLHCLGRCLFDTRLQPWKFVGTMADITAHKKASEEIILDRNRMRSLIDAQNSL
                     LIRMDKSGRVIYSNLSFRKYFGLENMCSNSIWESPFLHSDQRDLLQKTLDKCFSQPGV
                     PQMLLLQLKNAKGESKMHEWELVGIENNTGLTDEVQGIGRDVTSQYQLTSRIQQANSN
                     LESLINNFHSISIWSVDLEYNLTTCNCRMCGDFELFYNSKINRGANIIQALNAFPEVQ
                     EFWKIQYNRAFKGEQFDLELELNNRFFEVSFNPIRIGDEITGAAIYGMEITESKKSER
                     DLRLSEERLHFAIEGNQYGVWDYDVVHDHLYLSASCRAMLGMEVNDASHNLKDWSQII
                     HPDDLAEVVQVRKNLLEGVVDGFRSEVRMRTTSGIYKWIMDKGKTYERDADGKPTRVI
                     GLFLDISKNKADEAKLNEYLQRLEKFAYITSHNLRLPVANIIGLSNMIDEKSLKTKED
                     ETLIRKIRSSAQQIDDVIKEMNEAISFSNQQARQPQLTSIQNIWFIDDDDINNMLSER
                     LINKHFPNTGCKTFLDAEEALEILQKTPNQKPDAIFLDINMPRMNGWEFLEELQKLNV
                     SVNVYMLTSSIDPRDQQKAHEYKQVKDFISKPLREERLRLIVE"
     gene            complement(5272..>7002)
                     /locus_tag="PK281_07535"
     CDS             complement(5272..>7002)
                     /locus_tag="PK281_07535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOY49236.1"
                     /translation="QSSDALRTHKGTALSNQFTNLFLDANFPRKNWASKAGLKNGFTV
                     PVNTGGRTVALMTFFSEGIDMNAESEIIHLVRMLANHVGVHIESSRLSYDFNQVFELV
                     PDFLCVLDSSGRFFRANKHLRQTIGMTAEELTKHSFFDFIDETYLDTALDFFHRLKSE
                     LLIRFEIAFISGVNRLLLEWSLSYNPEEGVIYGAAKDVTLRVKYDEELRLNNERFQLI
                     SRATNDVIYEWDIKHNYIEWGENFKRLFGHSEVSKYNTYEGWGTFLHPGDIERVNNNL
                     QAALYHKSSFLWSDEYRYQTADGSYKFILDRGTFIRNEKGEALKMIGAMQDITALKES
                     EETLIRLNDALQSRARQLQGFNKELEQFAYIVSHDLQEPLRMISSFMQLLLSSNDIEK
                     NEKSEQYINFAIDGAVRMKRLIQDLLTYSRIGTTEEDFVEVNTTEVLHETLLVYQQVI
                     QDKNAEIIAEPLPVIRGIASLIGQIFDNLLSNALKYSDKTRPIIHISASTTDTHHVFC
                     FRDNGIGIDPRHFDTIYLPFKRLHNRNEYSGTGIGLAVCKKIAEKHTGQIWVESKPGD
                     GSAFYFSVMR"
BASE COUNT         2280 a         1345 c         1195 g         2182 t
ORIGIN      
        1 acccaataaa aaatcccgaa tgattcagtt cattcgggat ttttttatgc cccaaaacgc
       61 tcattaaatg atgaaatatg agtggttaat tgtgctcaat ttcagtatct atccacttgc
      121 tcttaaccgt taattgaatt aagaacataa cgtttataaa gggaatttcc tctatattat
      181 agcataaaaa agcccctgaa aaatcagagg cttatttata tggttatgaa aatattaaat
      241 cgcaggtaat tgaattttga aaatagaacc taatccaaga gttgattcta cttcaatagt
      301 tccttgtaat ttctgcactg catctttcac gttagataat cccaatccat tactatcact
      361 tcgatcactc aatttgtaat acgaatcaaa gatgcgtttg tgatgttcag ctgctattcc
      421 aattccatta tcctccactt ctatttctaa caataaagga tattctttac aagtaacttt
      481 tacaaaacaa tctagtttac ttggatcgga atatttgatt gcgtttgaaa tcaaattact
      541 caatattaca cgtagtcgtg ctggatcaga attcaaatta acattcggac caatttcatt
      601 gacaaatcga attgtttcag caccaggaac gaatcgtaat tcatccatta catcttgaac
      661 ttgttcaaac aaatcaactt taactgctac aacttgtcct cggcttcgaa gtgatgcaat
      721 aatatcgtta gtaaatgttt ccagtttctt gagactcttt tccatcatat cgagataatt
      781 cccgaattct tctggatttt tctcttctct agctaatgta attaagccac taaccgagtt
      841 catcggagct tttaagtcat gcgataatat cttcaccaat cgatcgagct ccttattatt
      901 attctcaatt tcgaggaatt gaccacggaa tttctcatcc gccattttcc gctcagtaat
      961 attcatcata ctcacacagg cacctaaaac attttgtttt tcatccgaaa ccggactcaa
     1021 actgatgcta taccaatgta cggatgtagc tgaaaaccga taagccattt cccattgaac
     1081 ttgcttatcc agcatcgatt cttcaaactt cgctcgaaat gaatcttgaa catccgctag
     1141 caaataatca ctaaacggaa tattttcttt caactcctgc cccatcaact gaatattttg
     1201 tcgattagca ataacgttga atgctttgat gcgttgagat tgatccaaaa ggacgatact
     1261 ttgatttaca ttgttgaaaa tagaattcaa cattgcaacc gtttctttct gtttctcgct
     1321 gaaatagcgg ctttgagtaa tatccttaca aacacgtata attccgacta tcattccatt
     1381 ctcatcgtat aaatgagatg ctttgatatc ggaccaaaca attttatcgt ctttagtaat
     1441 taagcgtgtt tcccactgtg ttattcgacc agctaatatt tcgtgctgct cggctcgcac
     1501 attgagttta tcgacttcgg catataaaac gtcaaagttt ctacccacga tttcatctct
     1561 ggaataacca aaacttaact cggctccttc attccagtag gtgatgattc ctttacgatc
     1621 ggtaacaaat atggtgtcgc gaacattttt caatacaccc gactgaaaca acatttcctc
     1681 ttgaacatga cgtttttcag tcacatcatc ggctaatacc agataacaaa cttcaccatc
     1741 aagttccgtt tcctgaacac gacattcaac tgttactatt tttccatcct tacatacatg
     1801 atgaaaaact tcagcagtac tataatctcc atagcgttca cgaatcatcc gcttaagtgt
     1861 ttccacttct tcgggaggat gcaaatcgct cacttgcatt ttaagaaatt cctcttcgga
     1921 atatccaaaa tggtcgatag ccgattggtt gacttttaag attcgaagtc catctttttc
     1981 cctgatatac atgggaattg gattgttctc aaataaaagt ttgtaggaag cttggctttg
     2041 acgaatcaac tccatactta acttccgttc gatactatat aatatggatt ttgctaggag
     2101 tttttcttcg aattcgcctt taacaaggta atcttgtgca ccattcttaa ctgcttcatg
     2161 agcaaaacgt gtgtcgttta ggcctgagag tatcagaatg ggtatagctg gactaattcc
     2221 atttaatcgt tgaaaggaat caaatccgta tgaatcaggt aaagagagat caagtaaaat
     2281 tacatctggc tcactcgttt gcaccgaatg aattgcatcc tgcaaagagg ttacatgtat
     2341 tatgtcttta gaaaaatctc gtatttcatg gagcatctgc tgaattagca gaaagtcgcc
     2401 agtattatct tctacgataa ggatctgaag tctctgtgtt ttcatatcat ccttatctgt
     2461 tgacacacaa acacaaattt cctaattcca ctaggcttgt agtgggatag aaattcagtt
     2521 tgagggatag aaacccgaaa aagtacgtgt ggttcatgca tccatttgta aataatacta
     2581 cgcgaataat aaggtaaagg ttacggtatc agattgaaac taaaatagta aatccaattg
     2641 ggctacatga aacttccaca taaactttaa agatttttga attatcaaat ttaattgtat
     2701 tttcatgcaa taatcatttt ctgaaaaagg aggcagacaa atgatcacac tggctattaa
     2761 tctgtaccaa cagcttttcg atcagggaat tattccccga tgtttagtca cgctcgatgg
     2821 tgacttcgtg ctcactaaca gcgctttccg taagatgttc caattaactg ggacacatat
     2881 ggaattgaaa aacttcaatt tatttgatgt tttatcggat gcaacgggaa ttcataaaga
     2941 gagaatccgg aaggaacaac tcttttatag tgataccgat gaggcgatcg aaggaaagat
     3001 aataattttg aataatgatg gttcaaaaac gaaactcgat tatttaagaa gtcgggttga
     3061 gatggaaaat ctctctatgc tttcgtttga atggcaggat gttacagaga aagttgaata
     3121 ccgaaaaagt cttagaagta catacgaacg tcttcaatta gctgcatcta tcagcaacct
     3181 aggtgtttgg gaatatgagt ttgaaacaaa agaatggtat ttaaatgagt ggatgtgtgc
     3241 cttatttacg atacctgctc atggtaaaac tgatataact caaagtatag ttgcatctgt
     3301 tattccagaa gatttagagc tcttatctca aaaatggata gcagctaaag aaaaaggaga
     3361 tcatcttgat tttgaattta gaatcgttct ccccgactca ggtgtacatt atcttcattg
     3421 tttaggcaga tgtttatttg atacccgact tcaaccttgg aagtttgtag gtaccatggc
     3481 cgatatcaca gcacataaaa aagcttccga agaaattatt ttagatcgca atcgcatgcg
     3541 ttctttaatt gacgctcaga attctctatt aattagaatg gataagagcg gtcgagttat
     3601 atacagtaac ttaagtttcc gaaagtattt tggactcgag aatatgtgtt ccaatagcat
     3661 ttgggaatct ccatttttgc attcggatca acgcgattta ttacaaaaaa cattagataa
     3721 atgttttagc caaccgggtg ttcctcaaat gctattactt caacttaaaa atgcaaaagg
     3781 tgaaagtaaa atgcacgaat gggagcttgt aggaatagaa aataatactg gcttaactga
     3841 tgaagttcaa ggaataggtc gagatgttac atctcaatac caactaacct cgcgaatcca
     3901 acaagcaaat agtaatttag agtcattaat aaacaatttc cattcgatta gtatttggtc
     3961 ggttgattta gaatacaact taacaacctg caactgcaga atgtgcgggg attttgagtt
     4021 gttttataat agcaaaataa atcgaggtgc taatattata caagctctta atgcttttcc
     4081 tgaagttcaa gaattctgga aaattcaata taatagagct tttaaaggtg aacaattcga
     4141 tttggaactt gagttaaata atcgattttt cgaagtaagt tttaatccga ttcgaattgg
     4201 cgatgaaata acaggagctg ctatatatgg aatggaaatt actgaatcca aaaaatcaga
     4261 acgtgattta agattaagtg aggaacgttt acattttgca atcgaaggaa atcaatatgg
     4321 tgtttgggat tatgatgtag tgcatgatca tttatatcta tctgcttctt gtcgagcaat
     4381 gctaggaatg gaagttaatg atgcgagcca taatttgaaa gattggtctc agataattca
     4441 tccggatgat ttagctgaag ttgttcaagt aagaaaaaat ttgcttgaag gtgttgtaga
     4501 tggatttagg agcgaggtaa ggatgcgaac aacatcggga atttacaaat ggatcatgga
     4561 taaaggcaaa acctatgagc gtgatgcaga tggaaaaccg acacgcgtaa ttggattatt
     4621 cttggatata tctaaaaata aagccgatga agctaaacta aacgaatacc tgcaacgact
     4681 agaaaagttt gcctatataa catcacacaa tcttcgctta cccgttgcca acatcattgg
     4741 tttatcgaac atgattgatg aaaaatcact caaaaccaaa gaagatgaaa cacttatccg
     4801 aaaaattaga agttcggcac agcagattga tgatgtaatc aaagaaatga atgaagccat
     4861 ctcattttct aatcaacaag ctcgacaacc tcaacttaca tctattcaga atatttggtt
     4921 tattgatgat gacgatatca ataatatgtt gagtgaacgt ttgattaaca aacatttccc
     4981 aaatacaggt tgcaagacat ttttagatgc tgaagaagca ttagagattt tgcaaaaaac
     5041 acccaaccaa aaacctgatg caattttctt ggacatcaac atgccgcgca tgaatggatg
     5101 ggaatttttg gaagaacttc aaaaactcaa tgtatctgta aatgtatata tgctaacatc
     5161 ttctatcgat ccacgcgatc aacagaaagc acatgaatac aaacaggtaa aagattttat
     5221 atccaaacct ttaagagaag aacgtttacg cttaattgtt gaataattca attagcgcat
     5281 cacgctaaaa taaaaggcac ttccatcacc aggtttactt tcaacccaaa tctgaccagt
     5341 atgtttttcg gcaatttttt tacacacagc taagccaatt ccagtacctg aatattcgtt
     5401 cctattgtgt aaacgtttaa atggtaaata gatcgtatca aaatgccgag gatcgatccc
     5461 aattccatta tcccggaaac aaaaaacatg atgcgtatca gtagtgctgg ctgatatatg
     5521 gataatcgga cgagtcttat cagaatactt caaagcattc gatagtaaat tgtcgaaaat
     5581 ctgtccaatt aacgaagcta ttccccgaat tacgggtaaa ggttcagcaa ttatctccgc
     5641 atttttatct tgaattactt gctgataaac gagcaaagtc tcatgtaaaa cttcggttgt
     5701 atttacctca acaaagtctt cttctgttgt accaattctt gaatacgtaa gcaaatcttg
     5761 gatgagtcgt ttcatcctaa cagctccatc gatagcaaaa ttgatatact gctccgattt
     5821 ctcgtttttc tcaatatcgt tgctcgaaag caataattgc ataaagctcg aaatcatccg
     5881 caatggctct tgcaaatcgt gagacacgat gtatgcaaac tgctctaatt ctttattaaa
     5941 accttgtaac tgacgagctc tagattgtag cgcgtcattc aaccgaatca aggtttcttc
     6001 actttccttc agagcggtaa tatcttgcat cgctcctatc atctttaaag cttcaccttt
     6061 ttcatttcga atgaaagttc ccctatctaa tatgaattta taactaccat cagctgtttg
     6121 ataacgatat tcatcactcc ataaaaagga agatttatgg tataaagcag cttgtaaatt
     6181 attattcacc cgttcaatat ctcctggatg caaaaatgtt ccccagcctt catacgtatt
     6241 gtatttggat acttctgagt gaccaaataa acgcttgaaa ttttcacccc actcgatata
     6301 gttgtgcttg atgtcccatt cataaatcac atcattcgtg gctcttgaaa tcagctgaaa
     6361 gcgctcgtta ttcaaacgca actcttcatc atatttcaca cgaagcgtaa catctttcgc
     6421 tgctccataa ataacgcctt cttccggatt atacgataaa gaccactcga gtaaaagtcg
     6481 gttcacaccc gatataaatg caatctcaaa ccgtattagc aactccgatt ttaggcgatg
     6541 aaaaaaatca agtgccgtat ctaaataagt ctcatcaata aaatcaaaaa aagaatgctt
     6601 ggtcaactct tctgcagtca taccaatggt ttgccgcaga tgtttattgg ctcggaaaaa
     6661 tcttccagaa ctatccaata cacacaaaaa atctggaact aactcaaata cctgattgaa
     6721 atcgtatgat aatcgactac tctcaatatg aacgcccaca tgatttgcca gcattcgaac
     6781 taagtgaata atctccgatt cggcattcat atcaatacct tcggagaaga aagtcatcaa
     6841 ggcaaccgta cgaccacctg tattaacagg aacggtaaat ccgtttttca atcctgcctt
     6901 tgatgcccag ttctttctag gaaaattagc atcgagaaat aaattggtaa actgatttga
     6961 taatgctgtt cctttatgcg tccttaatgc gtccgaactt tg
//
LOCUS       DAOOQW010000119         6963 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_13318_length_6963_cov_3.861899, whole genome shotgun sequence.
ACCESSION   DAOOQW010000119 DAOOQW010000000
VERSION     DAOOQW010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 6963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6963
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13318_length_6963_cov_3.861899"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="PK281_07540"
     CDS             <1..221
                     /locus_tag="PK281_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49237.1"
                     /translation="KIMDAISPISAIPLCTSSFAMRCAGLSSAQLPDPAQTKTTGLNN
                     SFIGQFGLESDESVANTGLFFQFGKHQK"
     gene            428..787
                     /locus_tag="PK281_07545"
     CDS             428..787
                     /locus_tag="PK281_07545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOY49238.1"
                     /translation="MLSETDAKGEILFVNESFCEISQYEWRELVGKSHSIIRHKDMPE
                     KLFMHMWKEIEQGKVFRGILKNKAKDESHYWVRTTIMPVYGNLGKITKYLGGTYPIRD
                     ERVAEELYREQSKILQF"
     gene            942..1805
                     /locus_tag="PK281_07550"
     CDS             942..1805
                     /locus_tag="PK281_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015482077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HOY49239.1"
                     /translation="MRQLKIVKQITNRETQSLDKYLQEIGRMDLITAETEVALTKRIK
                     SGDQAALEKMVKANLRFVVSVAKQYQNHGLTLGDLINEGNVGLIKAAMRFDETRGFKF
                     ISYAVWWIRQCIMQALADQSRVVRIPLNRVGSLSKLNRTFSQLEQTFQREPSSEELAE
                     ALDTTPEEVAHTFRTGSRQVSVHAPFVQGEENGLLDILENKDEETPDSGLMGDSLRTE
                     IQRSLTTLTKREAEVISLYFGLNGEHPKSLEEIGEMFQLTRERVRQVKENGIRRLRNT
                     SRSKTLKSYLG"
     gene            complement(1870..2199)
                     /locus_tag="PK281_07555"
     CDS             complement(1870..2199)
                     /locus_tag="PK281_07555"
                     /inference="COORDINATES: protein motif:HMM:NF012339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOY49240.1"
                     /translation="MNVGRKFDIGITVFLKGEKHAIATYQGEYRNLMALLYDKFFVED
                     FGECKGIGRCGTCHVHILGGKKEWLKREGNEPATLSKMDLVGPNSRLACQIMIDEKLD
                     GLRIEVV"
     gene            complement(2205..2675)
                     /locus_tag="PK281_07560"
     CDS             complement(2205..2675)
                     /locus_tag="PK281_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HOY49241.1"
                     /translation="MNTPIKRHASLKPLSHDHHHGLLLCWKIRTGYRKNVEINRIKLY
                     ADWFYKNHLLPHFEIEEKYVFPILGNEHELVKRALSEHRKLRRLFEDFEQVSKTLGMI
                     EEKLEAHIRFEERFLFNEIQNTAPPKVLEEMMAIHSGLNSGTRSVEEWEDEFWK"
     gene            complement(2725..3456)
                     /gene="ric"
                     /locus_tag="PK281_07565"
     CDS             complement(2725..3456)
                     /gene="ric"
                     /locus_tag="PK281_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein"
                     /protein_id="HOY49242.1"
                     /translation="MEINQSQIIGELVAQDYRTASVFKKYGIDFCCQGNRTIQDACEK
                     KSVDSKVVLSDLEKATQAQSESATDYKSWPIDLLADYIEKKHHRYVEAKTLEIKPYLD
                     KICKVHGERHPELLEIQKQFNASAGELAAHMKKEEFILFPFVRKMAKAKHEGLNVDAP
                     HFGTVQNPVQLMMSEHSTEGDRFRNIEGLSNNYTPPSDGCSTYRVTYALLKEFEQDLH
                     LHIHLENNILFPKAIELEKELTNKK"
     gene            complement(3467..3766)
                     /locus_tag="PK281_07570"
     CDS             complement(3467..3766)
                     /locus_tag="PK281_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin"
                     /protein_id="HOY49243.1"
                     /translation="MKTTGTPQMTILRSGKSFKVLQVTGSEGMSMPDHFSTKEAVLMI
                     QIGSATLKIKGEEHLLTKHHSFIIPAAEHHTLVLKENFQAIVIMEVDSEIKFVNS"
     gene            complement(3811..4245)
                     /locus_tag="PK281_07575"
     CDS             complement(3811..4245)
                     /locus_tag="PK281_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006990010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOY49244.1"
                     /translation="MFSKACEYGIKATSYIAVQSLDGHRVSLKDIAMSIDSPVAFTAK
                     ILQQLSKCDIIHSTLGATGGFEIERNRMEKIKLSQIVSAIDGDEIYKGCGLGLKECNA
                     KKPCPVHDKFKVIRDELKVMLEGTTVHELALGLKDGLTFLKR"
     gene            4556..4978
                     /locus_tag="PK281_07580"
     CDS             4556..4978
                     /locus_tag="PK281_07580"
                     /inference="COORDINATES: protein motif:HMM:NF014173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOY49245.1"
                     /translation="MFSKACEYAIKAMILVESCNNMERKISLAEIAEAIDSPVAYTSK
                     ILQQLRKNKFLISTIGAKGGFSIPKGRSVNLSEIVAVIDGDVFNRCVLGLPTCSSQNP
                     CPLHNQYVKIKESQYRLMLKTSIQEFSSSLKKKRISLK"
     gene            5053..6504
                     /gene="nirK"
                     /locus_tag="PK281_07585"
     CDS             5053..6504
                     /gene="nirK"
                     /locus_tag="PK281_07585"
                     /EC_number="1.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050421 - nitrite reductase (NO-forming)
                     activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-containing nitrite reductase"
                     /protein_id="HOY49246.1"
                     /translation="MFLTGGISITLAIVLTLSSCGTKTNDAKIGEDPAKMQATNSAEA
                     ELTAPPFVPKPVGNRAPMKLTVNMEIIEKEGEMADGVKYIYWTFGGSVPGSFIRTRIG
                     DEVEFHLKNHPDNKLPHNIDLHAVTGPGGGAASSFVAPGHEVVFSFKVINEGLYVYHC
                     ATAPVGMHIANGMYGLILVEPAGGLPPVDKEYYIMQGDFYTKGANGDKGLQPFDMAKA
                     IKEDADYVVFNGKVGALTDDNAITANVGERVRLFVGNGGPNLVSSFHVIGEIFDNVHI
                     EGGSSINKNVQTTLVPSGGATIVDFKVDVPGTYILVDHSIFRTFNKGTLGMLKVSGEE
                     NKTIYSGTKADNVYHPEGGTIQSMPTDENAKPQAKAVSLAERIAEGKSIYSKTCFACH
                     QPAGEGLPNAFPPLAKSDYLNAEVNRAIEIVLKGKTGELTVNGQKFNSVMTAQTLTDE
                     EVANVLTYVYNSWGNSKKDVTEAMVTSVRKKIK"
     gene            6588..>6963
                     /locus_tag="PK281_07590"
     CDS             6588..>6963
                     /locus_tag="PK281_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007650307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOY49247.1"
                     /translation="MNSTQENNINQKQARQTVVASKDLPFEATTEMVTILGGEYIPLY
                     GRDSIRVHVNDLLMDVYPVTNENFAAFVKKNRRWGKSQIKKIFADDNYLMHWQNDTTL
                     GISMNQQSPVTNVSWYAAKAYCE"
BASE COUNT         2050 a         1497 c         1387 g         2029 t
ORIGIN      
        1 ctaaaatcat ggatgctata tcgcccattt ccgctattcc tctttgcaca agttcatttg
       61 ccatgcgctg tgctggctta tcttcggcac aactacctga tccggcacaa acaaaaacta
      121 caggcttaaa taattccttc ataggtcaat ttggattgga gagtgatgag agcgttgcca
      181 atacgggttt gttttttcag tttggcaaac atcaaaagta gcccgctcgc aaaatcgtga
      241 ggctgataga aatcatatcg taacctgatt aaggtcacac ctcttttata agaataagat
      301 taggattacg ctgccattgg ccatttgatt gcgactacac aaattatttt aaccgtatcc
      361 cccataacgc attctgggtt tcatcctgat catgaagaaa gatccgagat tctgtataaa
      421 ctctactatg ctttcagaaa cggatgcaaa aggcgagatc ctattcgtca acgagagttt
      481 ttgtgaaata tctcaatacg aatggcgcga acttgttggt aaatcgcaca gtattattcg
      541 acacaaggat atgccggaaa aattattcat gcacatgtgg aaagagattg agcaaggaaa
      601 agtttttcga ggcatactaa aaaacaaggc taaagatgaa tctcactatt gggtacgcac
      661 gaccattatg ccggtttatg gaaaccttgg taaaataacc aagtatttag gtgggacgta
      721 tcccattcgg gacgagagag ttgctgaaga actctaccgt gagcaatcta aaattctgca
      781 attctaatcc ttgcttacaa gaaatacgac ctcatcactt ttaaccctca ccaaaactac
      841 cgtaaccctg agcgatgcat ccccctccaa attcttcctg atcaatttca gtgaaatatc
      901 aaaaccccga acaaccataa attctactta ttaccacccg gatgagacaa ttaaaaatcg
      961 tcaaacagat taccaatcgt gaaacgcaat cactggacaa gtatttacag gagattggca
     1021 ggatggattt gattactgct gagacggaag ttgcgctgac aaagagaata aaatcaggtg
     1081 atcaggccgc gcttgagaaa atggtgaagg cgaatcttcg atttgtcgtt tcagttgcca
     1141 agcaatatca aaatcatgga ctaaccttag gtgacttgat taacgaggga aacgtgggat
     1201 tgatcaaagc agccatgcga tttgatgaaa cccgagggtt taaatttatt tcatacgcgg
     1261 tatggtggat acgtcaatgc atcatgcaag ctctggcaga tcaatccagg gtggtacgaa
     1321 ttcctttgaa tcgtgtgggt tcactttcaa aattgaacag aactttctca caactggagc
     1381 aaacctttca acgtgaacct tcttcagaag aacttgccga ggcattagac accactcccg
     1441 aagaagtggc gcatacgttt agaacgggat ctcgtcaggt ttccgtgcat gctccatttg
     1501 ttcaaggaga ggaaaatgga ttgcttgata tattagaaaa taaggatgaa gaaacacctg
     1561 actcaggatt gatgggcgat tctttgcgaa ccgaaataca acgatccctc acaacattaa
     1621 caaaaaggga agcagaagta atctcgcttt actttggact gaatggagaa catcccaagt
     1681 cactcgaaga gattggagaa atgtttcagc ttacccggga gcgcgttcgt caggtaaaag
     1741 agaatggaat ccgaaggctt agaaatacct ccagaagcaa gaccttaaaa tcatatttgg
     1801 gttagtgtgt atttctgtga catattgaac accacaaacc cacactctat attatatgta
     1861 cacgctgcgc taaactacct caatccgcaa accatccagt ttttcatcaa tcattatctg
     1921 gcaggccaat cggctattgg ggcccactaa atccattttg cttaatgtgg ctggctcgtt
     1981 accttccctt tttagccatt cctttttacc acctaaaata tgaacatgac atgttccaca
     2041 tcgtccaatg cctttgcatt ccccaaaatc ttctacaaag aatttgtcat atagcaaggc
     2101 cattagattt cgatactcgc cctggtaagt tgcgatggcg tgcttctccc ctttcagaaa
     2161 tacagtaatg ccaatatcaa acttccgccc cacattcatt gacgttattt ccagaattca
     2221 tcttcccatt cttcaacaga acgcgtacct gaattcaacc ccgaatgtat tgccatcatt
     2281 tcttccagca cctttggtgg cgctgtgttt tgaatctcgt tgaatagaaa cctctcttca
     2341 aatcgtatat gcgcttccaa tttctcttcg atcatgccaa gggttttact tacttgttca
     2401 aagtcttcaa acagtcttct caactttcga tgttccgaaa gtgccctctt cactagctcg
     2461 tgctcattcc ccaaaatagg aaatacgtat ttttcttcta tttcgaagtg cggaagcaaa
     2521 tgatttttat aaaaccagtc cgcatacaat tttatgcgat tgatttcaac attctttctg
     2581 taccccgtcc tgattttcca acaaagcagc aagccgtgat gatggtcgtg gctgaggggc
     2641 ttaagagatg catgacgttt aattggcgta ttcatggtgc cgcaagttta cgatcattgc
     2701 ttcgcgcgct gactcaacta tacattactt tttattcgtc agttcttttt ccaattcaat
     2761 cgcttttggg aatagaatgt tattctccaa atgaatatgg aggtgtaaat cctgttcaaa
     2821 ttctttaagc agagcatacg taaccctgta ggtgctgcag ccatcactcg gaggagtata
     2881 gttattactt aatccttcaa tgtttctgaa gcggtctcct tctgtgctgt gttcactcat
     2941 catcaattgt accgggtttt ggaccgtacc aaaatgagga gcatctacgt ttaagccttc
     3001 atgttttgcc ttcgccattt tccgcacaaa gggaaataaa ataaactctt cttttttcat
     3061 gtgggccgct aattcccccg cagaagcatt aaactgtttt tgtatctcca ataactcagg
     3121 gtgacgttca ccatgcactt tgcagatttt atctaaatac ggttttattt ccaatgtctt
     3181 cgcttcaaca tagcggtggt gttttttctc aatataatcc gccaacaaat caatgggcca
     3241 cgatttataa tccgtagcac tctcactttg tgcctgtgtg gctttctcta aatcgcttag
     3301 caccactttc gaatccacac ttttcttctc acatgcatcc tgaatcgttc tattaccctg
     3361 gcaacagaag tctattccat atttcttgaa tactgatgct gtgcggtagt cctgtgctac
     3421 caactctccg atgatttgac tttgatttat ttccattttt tttcgtttat gaattaacaa
     3481 acttaatttc cgaatcgact tccataatga caatggcctg gaaattctcc tttaagacca
     3541 gtgtatgatg ttccgctgcc ggtataatga aggaatgatg ttttgtcagc agatgttcct
     3601 ctccttttat tttcagggtt gccgatccga tttgaatcat gagcactgct tctttggtac
     3661 tgaaatggtc tggcatgctc atcccttcgc ttcccgttac ttgtaatact ttaaatgact
     3721 tacccgaacg gagaattgtc atttgtggtg tgcccgttgt tttcatttcc tgaatagttt
     3781 taaccgttgt ggtatcgctg tctccatgaa ttatcgcttc aaaaaagtca gcccgtcttt
     3841 taaacctaaa gcaagttcat gtacagtcgt gccttccagc ataaccttga gctcatcgcg
     3901 aattacttta aacttgtcgt gaacggggca tggtttcttc gcgttgcatt ctttcaagcc
     3961 caaaccacat cccttataaa tttcatcgcc atcaattgcc gaaacaatct gactgagttt
     4021 aattttctcc attcggttac gttcaatttc aaatccccca gttgcaccaa gagtagagtg
     4081 tatgatatca catttagata gttgctgtag aatcttggct gtaaacgcca ccggtgaatc
     4141 gattgacatt gcaatgtctt tcaagctcac gcgatgacca tccaaagact gaacagcaat
     4201 gtagctggtg gcctttattc catattcgca cgcttttgaa aacatactgc ctctgtattt
     4261 gcatgcaaga tacgtaattc ttttatctcg gacaatttta tccgagataa aagataacta
     4321 tcgctgagtg gctgaaatac atacagttag cttcggtatt aatccgatta ctttcccatt
     4381 agtacgttct agttaaaccc attcctgaac tttaacaggg atgaaggtta agaaggcatc
     4441 atgtttcgtt gatccttcat ttctgaaatc cataaaattt actcaacaat ataatttgga
     4501 taataacgtc cgaattaaaa tacttttaca taatcttgta gacaaagaat agcggatgtt
     4561 cagtaaggca tgtgaatatg ccattaaggc gatgattctt gttgagtcct gcaacaacat
     4621 ggagagaaaa atcagtttag cggaaatagc tgaagctatc gattcgcccg ttgcttatac
     4681 atcaaaaatt ctccagcaat taagaaaaaa caaattcctg atttcaacta ttggggcaaa
     4741 gggtgggttt tcaattccga aaggaagaag tgtaaacctt agcgaaattg tagccgtgat
     4801 tgatggcgat gtgtttaatc ggtgcgtgtt gggattacca acctgttcat cgcagaaccc
     4861 ctgccctttg cacaatcagt atgttaaaat aaaagaaagc caatacaggc tcatgcttaa
     4921 aacatccata caggaatttt catcatcctt aaagaagaaa cgcatctcac taaaatgacc
     4981 gataaaataa cgtgtagata aaatacttaa acaaaacaaa aagaaataat gctacctaaa
     5041 tcacgaaaac agatgtttct cactggagga atatctatta cccttgccat cgttttaaca
     5101 ttgtcaagtt gtggcaccaa aactaatgac gcaaaaattg gagaagatcc tgcgaagatg
     5161 caagcgacaa actctgcgga agccgaatta accgcccctc cttttgtgcc caagccggtt
     5221 ggaaacagag cgcccatgaa gcttacggta aacatggaga ttattgaaaa agaaggtgaa
     5281 atggcggacg gggtgaaata tatctattgg actttcggtg gctctgttcc tggaagtttt
     5341 atccgtacgc gcattggtga tgaggttgaa tttcatttaa agaatcatcc cgacaacaaa
     5401 ttgccacaca acatcgattt acatgccgtt acgggaccgg gtggtggagc tgcttcatct
     5461 tttgttgctc ccggacatga agtggtattt tccttcaaag taattaacga gggattgtac
     5521 gtttatcact gcgctaccgc gcccgttgga atgcacatcg ccaatggtat gtacggtctt
     5581 atcctggtgg aacctgcggg cggtcttccc cctgtcgata aagaatacta catcatgcaa
     5641 ggtgattttt atacaaaggg agccaatgga gataagggtc ttcagccttt cgatatggca
     5701 aaggcgataa aagaggacgc agactatgtc gtatttaatg gtaaagttgg agccctgacc
     5761 gatgacaatg caatcactgc caatgtaggg gaacgagtac gcctgttcgt tggcaacggt
     5821 ggtcctaatc tagtgtcttc ctttcatgtt attggcgaaa tattcgacaa cgtccacata
     5881 gaaggaggct catcgattaa taaaaatgtt caaactacat tagtcccatc aggaggagca
     5941 accattgtcg attttaaggt cgatgttccg ggcacttaca ttctggttga tcactcgata
     6001 ttccgtacgt ttaacaaggg tacattggga atgttgaaag ttagtggtga agaaaataaa
     6061 accatttatt caggtacaaa agccgataac gtttatcatc cggaaggagg cacaatccaa
     6121 tctatgccga ccgatgagaa tgctaaacct caggccaagg cagtctccct cgctgaacga
     6181 attgctgaag gaaaatccat ctacagtaaa acctgctttg cctgtcatca gccagccggt
     6241 gaaggtttgc ccaacgcatt ccctcctttg gcgaaatcgg attacttaaa tgcagaagtc
     6301 aaccgagcca ttgaaatcgt attgaaagga aaaacgggag agctaacggt gaatggacaa
     6361 aaatttaaca gtgtgatgac tgctcaaacg ctcactgacg aagaggttgc caatgtcctt
     6421 acctatgttt acaactcatg gggcaatagt aaaaaagatg ttacggaagc catggtgaca
     6481 tccgttcgga aaaagataaa atagaaatcg taatcacttg aaaagtgtat tcatctttta
     6541 gtgtattcat cctttgcgga atatttctgg tgttctcttg ttcggaattg aattctacac
     6601 aggaaaacaa catcaatcag aaacaggcca ggcaaactgt agttgcttcg aaagatttac
     6661 ctttcgaagc aactacggaa atggtcacta ttttaggtgg tgaatacatt ccattatatg
     6721 gtcgcgattc catacgcgta cacgttaatg atcttttaat ggacgtttat ccggtgacca
     6781 atgagaactt tgcagcgttc gttaagaaaa acaggcgttg gggtaaatct cagataaaaa
     6841 aaatatttgc agacgacaac tatctaatgc attggcaaaa tgacactact ctgggaatta
     6901 gcatgaatca gcaaagcccg gttacgaatg tatcctggta tgctgctaaa gcgtattgcg
     6961 aat
//
LOCUS       DAOOQW010000120         6946 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_13360_length_6946_cov_4.211000, whole genome shotgun sequence.
ACCESSION   DAOOQW010000120 DAOOQW010000000
VERSION     DAOOQW010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 6946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6946
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13360_length_6946_cov_4.211000"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(91..1722)
                     /locus_tag="PK281_07595"
     CDS             complement(91..1722)
                     /locus_tag="PK281_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014166450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HOY49248.1"
                     /translation="MNSTSLKSSNSILQSLKQYWKSDLLSGFLVSLIALPLCLGIAGA
                     SNFPPIMGVLTAIVGGLVVSFFAGSELTIKGPAAGLIVICAGAVEELGKGNLETGWHL
                     TLAVVVVAGILQVVLGKLKVASLADFFPLSAVHGMLAAIGIIIMSKQLHLAVGILPAE
                     LKGKEPLELLMMVPHSLMNMEWHIAAIGGVSLLILFGWKYIGKGFLAKVPPALVVLIV
                     AVIMGQFFHLYDPIYQARKPLVNPGDFNINLNVDFAALTNLELLPIFLKYLLMFTLIG
                     SLESLLTGRAIDLLDPHKKKSDLSKDLSAVGIGNIVSGLLGGLPMISEVARSSANISN
                     GGRSRWANFFHGSFLLLFVVALVPFIKLVPVSALAAMLIFVGFRLASPKEFEHVFHVG
                     KEQLIIFLCTIIATLLTDLLIGIAIGIMVKFIIHSINGVPASSFFKPFLTMHVDEENE
                     IYTIEVQKSAIFSNFLGFKKQLERIPAGKHIIIDFEQTRLVDHSVMENLHNFENAYLR
                     TGGTFIITGLDGHTPLSKHSLASRKKAAFKPVTQK"
     gene            complement(1910..2257)
                     /locus_tag="PK281_07600"
     CDS             complement(1910..2257)
                     /locus_tag="PK281_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49249.1"
                     /translation="MNFRLFRTSLLLTLLFLLRFGLIQAEGYLSPQINRGFAVQASHP
                     VQQDVVCLPIEITEDNEINEARKRLISFAQAVIIHFELYYSLTVDAVTYNDCSSIPLT
                     DNPRWLKNCVFRI"
     gene            complement(2903..6184)
                     /locus_tag="PK281_07605"
     CDS             complement(2903..6184)
                     /locus_tag="PK281_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011708232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOY49250.1"
                     /translation="MHRILLTLLSIAVSVPVFAQKKSAGKTETKTEWNVNTPPGPAKS
                     VSFDLTEGTWMNLDVSPDGKTLVFDLLGDIYSMPSSGGAAVCLRSGYAYEVQPRFSPD
                     GSRISFTSDAGGADNIWVMNADGSNPKQITKEDFRLLNNAVWTRDGNYLIARKHFTST
                     RSAGAGELWMYHISGGSGMQLTTKKNDQQDLGEPFVSNDDRYVYYSEDVYPGGYFQYN
                     KDPNSQIYVIKRLDRITGSNDIVVRGPGGAVRPTLSHDGKHLAFVRRVREKTVLFVRD
                     LASGIESPIYSDLSKDQQEAWAIFGVYTNFNWSPNDQFIYIWAKGHIQKIGVKSGEVA
                     QVPFKLTSNHKITDALHFQQQAAPDSFDVKTIRQAILSPDGKYIVFNAVGYLWMKDLP
                     AGKPRRLTNTSHFEFEPAFSSNGEKLAWVTWSDTAMGAIIEMDLKTGALQKLNKEKGI
                     YRTPNYSPDGKQLVYQKEEGNDHQGFAWCNEPGIYLMNAQNGSSKRILTDGESPRFSA
                     DGQRIFYQTGGYLFGSLEKAFRSVDLTGNDERTHYKSTYANQYIPSPDNKWLAFTELF
                     KVYLTPLISTGKALDLSNGTTAYPVKLVSQEAGINLHWNKQSSSLNWTLGDQYYSTDV
                     KPWIDLKEGATAPKPVSKGLSIGLRLAHQKPSEKVAYTHARIVTMKDSLVIEDGTIIT
                     EGNRITQMGKSADIQPASDVKIVECDGKTIIPGLIDVHAHLGTFRYGLSPQQQWSYFA
                     NLAFGVTTTHDPSSNTEMVFSQSEMVKAGTMTGPRIFSTGIILYGADGDFRAQINNLE
                     DAQFALKRTKAFGAFSVKSYNQPRRNQRQQVITGARELGMEVVPEGGSHFFHNMSMIL
                     DGHTGIEHNIPVADIYDDVVKLWSNSKTGYTPTLIVSYGAVNGEYYFYEKDKVWENAR
                     LLNFTPRALIDSRARYRTAVPDEEYDNGHIQVARSCKKLADAGVKVNLGAHGQIQGVG
                     AHWELKMLASGGMSNYQALKCATVNGAYYIGMEKDLGTLEVGKLADFVVLDSNPLDDI
                     NNIGSIRYTVANGNAFDAMTMQSIHNMQARRLPFYFENMYGNNDFKWHDETHGFHNHM
                     CGCRK"
     gene            6480..>6946
                     /locus_tag="PK281_07610"
     CDS             6480..>6946
                     /locus_tag="PK281_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOY49251.1"
                     /translation="MVSNRCKMMVKEELKKLDLHYVLVDLGEVEIMEDLDKETRNQLK
                     AGLLKSGLELMDDKKSVLIEKIKNSVVELIHYTDERIKVNFSEYLSKKLNYDYTYLAN
                     LFSEVQGSTIEQYIISNKIERIKELIIYDELNITEIAWKMHYSSVAHLSNQFK"
BASE COUNT         2228 a         1390 c         1340 g         1988 t
ORIGIN      
        1 taccgccctg tccataagtt ggatccattt gagcaatact gatacttgtc cacactaaag
       61 cagacagtag aaaaagaaac tttttcataa ttacttctgc gttacgggtt taaatgcggc
      121 tttcttacga gaggcgagcg aatgtttcga taatggcgta tgtccatcta aaccggtaat
      181 gatgaaggtt ccaccagtac gtaggtatgc attttcaaag ttgtgcaggt tttccatcac
      241 actgtggtcg accaatcgag tttgttcaaa atcaatgatg atgtgttttc cagcagggat
      301 gcgttcaagc tgttttttga acccgaggaa attgctaaaa atggccgatt tctgaacttc
      361 gatggtatag atttcatttt cttcatccac atgcatagtg aggaatggtt tgaagaaact
      421 agaggccgga acaccattta ttgagtgaat gataaatttc accatgattc ctattgcaat
      481 acctattagc aaatcagtta gaagtgttgc aatgatggta cacaagaaaa taatcaactg
      541 ttcttttcct acgtgaaaca catgttcgaa ttctttgggt gaagccaagc ggaatccaac
      601 aaatatgagc attgctgcta atgcagaaac aggtaccagt ttgatgaatg gaaccaatgc
      661 tactacgaac aataaaagaa agctaccatg gaagaaatta gcccaacgac ttcgtccgcc
      721 attgctaata ttcgccgaac tacgtgctac ttctgaaatc attggcaatc cacctaaaag
      781 tccggaaacg atatttccaa ttcccacagc acttagatcc ttacttaaat ctgatttctt
      841 tttatgcgga tctaataaat cgattgcccg accagtgagt aaggattcca aagaaccaat
      901 caaagtgaac atcaataagt acttaaggaa aattggtagc aattccagat tagtgagagc
      961 agcaaaatcg acatttagat taatgttgaa atcaccggga tttaccaaag gtttacgagc
     1021 ttgataaatt ggatcgtata gatggaaaaa ctgccccatt atcacggcaa cgataagtac
     1081 aaccagagca ggaggaactt tcgcaaggaa gccttttcca atgtatttcc aaccaaatag
     1141 tatgagtaag ctaacaccac caatagctgc aatatgccat tccatattca tcaagctatg
     1201 tggaaccatc atgagcaatt caagtggttc tttacctttt agttctgcag gtaaaattcc
     1261 aacagcaaga tgcagctgtt tcgacatgat gataatccca atagcagcta acataccatg
     1321 aacagccgat aatggaaaga agtcggcaag tgaagcaacc ttgagtttac ctagaacaac
     1381 ttgaagaata ccagcaacta ctactacagc aagagtaaga tgccaaccgg tttctaagtt
     1441 gcctttccca agttcttcaa ctgcaccagc acaaataaca attaaaccag cagccggacc
     1501 tttaatagtt aattcagaac cagcgaaaaa agagacaact aaaccaccaa ctatggcggt
     1561 aagcacaccc ataataggtg gaaagtttga tgcaccagca atgcctaagc aaagtggaag
     1621 tgcaatcaac gagacgagaa atcccgaaag caagtctgat ttccagtatt gcttaagtga
     1681 ttgtaggata gaatttgagg attttaaaga agttgaattc atgggtactt taactttatc
     1741 ccaaaaggga atgaattaaa atgcagagaa tcgccataga cgaaatgctg ctaaaagttt
     1801 gtaaggccgt gaaatgaatt aagaatcagc acatggctga actgtgtaat gacacacaga
     1861 aagcattcat tcggctaaca gaaaaactga tttgaatcag tacccaattt caaattctaa
     1921 aaacgcaatt cttcaaccaa cgaggattgt cggtaagggg aatggaagaa cagtcgttgt
     1981 atgtaacggc atcaaccgtt aaggaataat aaagttcaaa gtgaataatg acagcttgtg
     2041 caaacgatat aagtcgctta cgtgcttcat ttatttcatt gtcttcggta atttcgatgg
     2101 gtaagcaaac aacatcttgt tgaacaggat gtgaagcttg aacggcaaat cctctattaa
     2161 tttgaggaga caagtatcct tccgcttgaa taagtccaaa gcgaagaagg aagagtaatg
     2221 ttagaagcag acttgtacga aacaacctga aattcatggt gcaaatgtat actttttaaa
     2281 accgaatagg tttcatagaa gcatcacctt aaaatggcgg gctggcttgg caataattat
     2341 gcctagaaat aaatccttga tgaaggaaaa gtactctggt gaataagcac aaccagactt
     2401 atccagaact acttttcctt caggctgaaa ctagaactaa gctttccgga taactttaat
     2461 acctgcaaat gcagatagta aagtagttag cgacaaaagc gcaaacaaaa tccaacttcc
     2521 agaacatgat cccgaaagag aactatttga actcatgccc acagcatccg aaagcaacct
     2581 taggttcaat ggcagcagaa acaaggcaag cagacaacta agtaaaccga acaaccttat
     2641 catattccga agatagatgt agcaagaagc tgttaatcag tcgagttaca gaaaggaatg
     2701 aaccagatag tgttaataac tacgatattt aattaattga tttactgtca tttacatgat
     2761 ttgatgtaag attttgatac atgggttata aaaaaatgcg ctccaacgaa gcgcattttt
     2821 tcaaagtttg agtatataat cttacaaaaa atattgccta aatcgcctta gtcaactgtt
     2881 agattgaaat aggtattaac ggctactttc tacaaccaca catgtgatta tggaaaccat
     2941 gcgtttcatc gtgccattta aaatcattat taccatacat gttttcgaaa tagaaaggta
     3001 aacgacgtgc ttgcatattg tgaatcgatt gcatagtcat ggcatcaaat gcattcccat
     3061 tagcaactgt gtatcgaata ctgccgatat tattgatatc gtctaaagga ttagaatcaa
     3121 gcactacaaa atcagctagt ttcccaactt ccagtgtgcc gaggtctttc tccatcccga
     3181 tataataagc tccattcacg gtagcacact tcaacgcttg ataattggac attccgcctg
     3241 aagctaacat ctttaattcc caatgtgctc caactccttg aatttgtccg tgtgcaccaa
     3301 ggtttacttt aacacctgca tcagctaact ttttacacga acgcgctact tgtatatgac
     3361 cattgtcgta ttcttcatct ggaactgctg tacgatatcg agcacgtgaa tcaatcaacg
     3421 cacgaggagt gaaatttaac agtcgggcat tttcccacac tttgtctttc tcatagaaat
     3481 agtactctcc attcaccgct ccataactca caatcaatgt tggtgtgtat cccgttttag
     3541 aattgctcca tagcttcacc acatcatcat atatatctgc aactggaatg ttatgctcaa
     3601 taccggtatg tccatcgagt atcatactca tattatgaaa gaaatgtgat ccaccttcag
     3661 gaacgacttc cattcctaat tcacgagcac ctgtaataac ttgctgacgc tgattacgac
     3721 gcggttggtt gtagctcttc acactaaaag ctccaaatgc cttcgtacgt ttgagagcaa
     3781 actgagcatc ttccaaattg ttaatctgcg ctctaaaatc tccatcagcc ccgtataaaa
     3841 tgatacctgt agagaaaata cgtggacctg tcattgtacc tgccttcacc atctcgcttt
     3901 gcgaaaacac catttcggta ttgctcgaag gatcgtgtgt agtagtaact ccaaaggcaa
     3961 gattggcaaa ataggaccat tgttgctgtg gacttaagcc ataacggaaa gttcctaaat
     4021 gcgcatgtac atctatcaaa cccggaataa tagtcttgcc atcacattct acaattttca
     4081 catcagaagc tggttgtata tcggccgatt tccccatttg agtgatccga ttgccttcgg
     4141 tgatgatggt tccatcttca atcaccaaac tatctttcat ggtaactatg cgtgcatgcg
     4201 tataagcaac tttttcactt ggcttttgat gtgctaaacg taagccgata gacaaacctt
     4261 tactaacagg tttaggagct gtagcacctt cttttaaatc gatccaaggt tttacatcgg
     4321 tactgtaata ttgatcaccc aaggtccaat tcagtgatga actttgttta ttccaatgca
     4381 gattaattcc tgcctcttga gaaactaatt taaccggata ggctgttgtt ccattactta
     4441 aatcgagtgc ttttccagtt gaaatcagcg gtgtgagata tactttaaac aattctgtaa
     4501 atgccaacca tttattatcc ggactaggaa tgtattgatt tgcataagtt gatttataat
     4561 gcgttcgttc atcatttccc gtcaaatcaa cacttcgaaa cgccttttct aaagaaccaa
     4621 ataagtaacc acctgtttga tagaaaatgc gttgtccatc ggcactaaaa cgaggacttt
     4681 ctccatccgt gagtatacgt ttgcttgagc cattctgagc attcatcaaa taaattccgg
     4741 gttcattgca ccaagcaaac ccttgatgat cgttaccttc ttccttttga taaaccaatt
     4801 gttttccatc gggcgaatag ttaggtgtgc gataaatccc tttttccttg ttgagttttt
     4861 gtaaggcgcc cgttttcaaa tccatttcga tgattgctcc catggcggta tcgctccaag
     4921 ttacccaagc tagtttttca ccgtttgatg agaaggctgg ctcaaattcg aaatggctcg
     4981 tgtttgtcaa acgacgaggt tttccggccg gtaaatcctt catccataaa tatccaaccg
     5041 cgttaaagac gatgtatttt ccatcaggag aaagaattgc ctgacggatt gttttcacat
     5101 caaatgaatc gggtgctgct tgctgctgaa aatgaagcgc gtcggtaatt ttatgattgc
     5161 tggttagttt aaaaggcact tgcgctactt caccactttt aacaccaatt ttctgaatat
     5221 gtcctttcgc ccagatgtag ataaactgat cattgggcga ccaattgaag tttgtgtaca
     5281 ctccaaaaat cgcccaagct tcttgttgat ctttactcaa atcggaataa atcggggatt
     5341 cgataccgct tgctagatcg cgaacgaaca aaactgtttt ctcccttacg cgtcgaacaa
     5401 atgctaagtg tttaccatcg tgggataaag ttggacgaac agcaccacct ggtccacgta
     5461 ccacgatatc gttacttcct gttatccgat caagtcgttt gatgacgtaa atctgtgagt
     5521 tgggatcttt gttgtattga aaataaccac ctggataaac gtcttcgctg tagtacacat
     5581 atcgatcatc attcgaaacg aatggttcac ctaggtcttg ttggtcgttc tttttagtgg
     5641 tgagttgcat tccactacca ccgctgatgt gatacatcca caactcccct gctcctgccg
     5701 aacgggtgct ggtgaagtgt ttacgtgcaa tgaggtaatt tccatcacgc gtccatacgg
     5761 cattgttgag taagcgaaaa tcttctttgg tgatttgttt gggattagaa ccgtcggcat
     5821 tcatcaccca aatattatct gcaccacctg catcactcgt aaacgatatg cggctaccat
     5881 caggactaaa tcgaggttgc acttcatagg cataacccga acgtaggcaa actgccgcgc
     5941 caccggaaga aggcatggaa taaatgtcac cgagtagatc aaatacaagt gttttcccat
     6001 cggggctcac atctaggttc atccaagttc cttcggtgag atcaaatgaa acgcttttag
     6061 caggaccggg cggtgtatta acattccatt cagttttggt ttcagtcttt ccggctgatt
     6121 tcttttgagc aaatacagga acagaaacag caatcgataa tagtgtaagg agtatgcgat
     6181 gcataggata ttttgatgaa aagtaaaagt atgtgaaatg gagatgaaca agaacaaata
     6241 cgggtaaaaa acacctagaa tgtttaattg taatggttga atgtatgtta aaagttctaa
     6301 ataagctgat ttagaactat tcaaaatgta ataacagtga taaatcgtgg tatcttgaag
     6361 agaattgaaa atgtgtgaat tatacaaatc attgggtact tcacataaga aacaggtggg
     6421 caataaggtt gaattttgac aggttaaatc aaactaattt gaaactctac ataaaataca
     6481 tggtgagtaa tcgctgtaaa atgatggtga aagaagagtt gaaaaaactt gatttgcact
     6541 atgtgttggt tgatttaggt gaagtagaga tcatggaaga cctcgataaa gagactcgaa
     6601 atcaattaaa agccggatta ttaaaatcgg gtttagaatt gatggatgat aagaaatcgg
     6661 tattaattga aaaaatcaaa aattcggttg ttgaattaat acactatacc gatgagcgga
     6721 ttaaagttaa tttctcggaa tatttaagca agaaattaaa ttacgattat acctatttag
     6781 ccaatttatt ttcggaagtt caaggcagta caattgaaca atatatcatt tcaaataaaa
     6841 ttgagcgaat taaagaactc atcatttatg acgaattaaa tatcaccgaa atcgcctgga
     6901 agatgcacta cagtagcgtt gcccatttat ccaatcaatt taaaaa
//
LOCUS       DAOOQW010000121         6923 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_13428_length_6923_cov_4.474228, whole genome shotgun sequence.
ACCESSION   DAOOQW010000121 DAOOQW010000000
VERSION     DAOOQW010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 6923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6923
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13428_length_6923_cov_4.474228"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..918
                     /locus_tag="PK281_07615"
     CDS             <1..918
                     /locus_tag="PK281_07615"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:NF014411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl glucosamine 2-epimerase"
                     /protein_id="HOY49252.1"
                     /translation="EILLREKPDWLVVYGDTNSTLAAAIAGSKIHVPIAHIEAGLRSF
                     NKKMPEEVNRIVCDHVSTLLFSPTPTGFNNLLLEGFKVNKPPFTADNPGVFQCGDLMF
                     DNSQYFASVASEKTTIAADLELNTRPFILATIHRNNNTDEPERLNALFGTLLELAEEA
                     GIRILLPLHPRTRKMLGELLNADLQEKLKNGWLQLIEPASFLEMIVLEQRAKMVITDS
                     GGVQKEAFFFQKPCIILRPETEWVELVQNGTARICDADPVRIREAFTYFLNHGSELNF
                     PPVFGDGKAADFILEQLYACTTAGKTILT"
     gene            941..2245
                     /locus_tag="PK281_07620"
     CDS             941..2245
                     /locus_tag="PK281_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOY49253.1"
                     /translation="MLLIYTPRITARNKYTFKLIFKEILGVTYEVTSDKTIFINWKGP
                     SISYCSKPVRDEIFFFATELLFENGIVVQEPGFLTWGGTKVFYPTVRNSALPFDPFAA
                     SFFLTSRYEEYLPHIRDQHGRFESKQSLAYLHGFLQKPVVNLWALAILQMIEDRYGKQ
                     TIKDRNYSFKPTIDIDNAWAFRHKGFIRAGGGMLSDLFKMNLKEFGERFRSVFHLQHD
                     PYDTYEYQIELIQRYKLNPLFFILLGDYATFDKNVPHQNKHMQSLIKFMGDFGDVGIH
                     PSYASNDQPEKLPKEIARLAYILHQEIHNSRQHFLRLSLPDTYQRLIECDITDDYTMG
                     YPDQPGFRAGICTPYFFYDLDLEIETALRLHPFAIMDGTLRDYMNIKPEDAADIINPL
                     IDEIKKVKGEFISVWHNESFAENERWKGWRKVYEDMIEKALA"
     gene            2276..3181
                     /locus_tag="PK281_07625"
     CDS             2276..3181
                     /locus_tag="PK281_07625"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOY49254.1"
                     /translation="MPHSLINKVLWDQAMKNSSNGLVYASTWYLDAVTNDNWDALVSE
                     DYSWLMPLPIKQKAGVKYLPTPLFIQQLGIFGPGPFTPELTDHFLKHIAANIKLVEYQ
                     LNSENLAPSSDIFEVKLRLNHLLNLPEDKSLLIKSYSENTVRNIKKAEKAQVRFGNAS
                     IRQMIKLFQDHKEHELENWKTENYNVLDKLYNACMLRQFGQSIGVYNAQGDLIAGAFI
                     TEWKDRATFIFSCNSPEGKESGALPALIHHYLTNAPEKINLFDFEGSDQEGLARFYKS
                     FGSIESNYVHLKLNRLPFYMRWFKH"
     gene            3185..3832
                     /gene="upp"
                     /locus_tag="PK281_07630"
     CDS             3185..3832
                     /gene="upp"
                     /locus_tag="PK281_07630"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011709450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HOY49255.1"
                     /translation="MSRILSNENSLVNVFISELRDETIQKDSMRFRRNLERLGEIIAY
                     EISKHLEYSEKEVTTPLGIANVPVLKDQPVIATILRAGLPVHQGMLNYFDQAGNAFVS
                     AYRKHHKDNTFEIKVEYMSSPDLNDKVLIICDPMLATGQSMLLAYKALIQRGKPRHVH
                     VVGLIASVEGLTYVKSHFPENTTFWMGAVDDELTVKSYIVPGLGDAGDLAYGTKI"
     gene            3897..4364
                     /locus_tag="PK281_07635"
     CDS             3897..4364
                     /locus_tag="PK281_07635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49256.1"
                     /translation="MTTAIQFVLLFLLCTVKFVVGLPAAYAAFNLGFLELTLFGSLAG
                     LTGVSAFMFFSDYLFRFWDAFRIRFFPPKKNVKRKIFSKSSRFYVKVIRKYGLAGIAF
                     ITPTIISIPVGTLLARRLYPNRKKVFLYLLVSVELWSLTFSAILHFPALLRNN"
     gene            4366..5052
                     /locus_tag="PK281_07640"
     CDS             4366..5052
                     /locus_tag="PK281_07640"
                     /inference="COORDINATES: protein motif:HMM:TIGR02254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HOY49257.1"
                     /translation="MEIKDLFFDLDRTIWDYETNSRETLDDLHEMFLERTVPGDKDTF
                     ARIFRFENSLLWDAFTANKIDKDYLRKNRFLNTIRRMGLSDPELAIRMEDAYLEVTPA
                     KKRLLPGASETLQVLAQKYRLHIITNGFEDVQNFKLINCGIRKYFNEVITSDGAKSRK
                     PNRDIFDYSLQLTGASIDESIMIGDDPEIDIVGAQKAGWQAILVNTMNLTHTLQVREV
                     ASFPELLNYL"
     gene            complement(5111..5446)
                     /locus_tag="PK281_07645"
     CDS             complement(5111..5446)
                     /locus_tag="PK281_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49258.1"
                     /translation="MKKILAILCLAAFTSSAIAAPLASVMNQGNVVVVKLDKDKDKDK
                     KKKGEKSCCSKDEKSAKSCHEGEGSKTGSSSATGSGHNGATEKKGACCSKDASKSCGK
                     GAKSAEPAK"
     gene            complement(5500..6159)
                     /locus_tag="PK281_07650"
     CDS             complement(5500..6159)
                     /locus_tag="PK281_07650"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020081730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HOY49259.1"
                     /translation="MTIHREGHRILLTLLIVLAIINVSLFSLFPDACLAQKLTGVASF
                     IIFILVLQFFRSPNRTQNLGDDWVLCPADGKIVAIEEVDEPEYYKGKRKQVSVFMSPL
                     NVHVNRYSISGTVTYFKYHEGLYLVASHPKSSTENERTTVVVENGKRSVLLRQIAGAV
                     ARRIVTYARVGDEAKQGGEFGFIKFGSRVDIFMPLDAEVQVKLGEVVKGGITIIAKFP
                     R"
     gene            complement(6218..6562)
                     /locus_tag="PK281_07655"
     CDS             complement(6218..6562)
                     /locus_tag="PK281_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOY49260.1"
                     /translation="MAIMITDECINCGACEPECPNNAIYEGGTEWRFSDGTSLSGEVN
                     AASGVFQAGATQKPVQMDTYYIVSDKCTECVGFHDEPQCAAVCPVDCCVDDPDHRETQ
                     EQLLAKKQYMHL"
     gene            6624..>6923
                     /locus_tag="PK281_07660"
     CDS             6624..>6923
                     /locus_tag="PK281_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49261.1"
                     /translation="MTSEQRISAFIQLGTEIYQTFHAPETELNIRQQQLLQLSDRMYH
                     SNGWFTFENTKHALLGISFMLKEENIRPWWNQYADQFPQEKLHAVGVIMAGNIPAV"
BASE COUNT         2097 a         1402 c         1320 g         2104 t
ORIGIN      
        1 gaaatcctcc tgcgagaaaa acccgattgg ttggttgttt atggtgatac aaattcgact
       61 ttagctgctg ctattgctgg ttcaaagatt catgtcccca ttgcacatat cgaagctgga
      121 ttacgctctt tcaacaagaa aatgcccgaa gaggtgaatc gaattgtatg tgatcatgtt
      181 tccactttac tcttctctcc tactccaact ggctttaaca acctactact ggaaggattt
      241 aaagtgaata aaccaccttt cacagccgat aatccgggag tttttcaatg tggtgatttg
      301 atgttcgaca atagtcaata tttcgcatct gtagcttcag aaaagactac tatcgctgca
      361 gatttggaac taaatacgcg tccgtttatt cttgctacaa tccacagaaa taacaatacg
      421 gatgagcctg aacgcttgaa tgcattgttt ggcaccttac tcgaattagc tgaagaagct
      481 ggcattcgaa ttttattgcc tttgcatccg cgaacgcgta aaatgttggg tgaattatta
      541 aatgccgact tacaagaaaa attgaagaac ggttggctgc aacttatcga gccagcttct
      601 tttctagaaa tgattgtcct agaacagcgt gccaaaatgg ttattacaga ttcgggagga
      661 gttcagaagg aagcgttctt ctttcaaaag ccttgcatca ttttacgtcc cgaaaccgaa
      721 tgggtagaat tagttcaaaa tggaactgcc cgtatttgtg atgccgatcc tgttcgtatt
      781 cgagaagctt tcacgtattt tctaaatcat ggttcggaac tcaacttccc acctgttttt
      841 ggagatggta aagcggctga ttttattctc gaacagctct atgcttgcac aacggcaggt
      901 aaaactattc tgacttagct ttaatctttg tcgatagagc atgttactca tatacactcc
      961 gcggattact gcccgaaata aatacacctt taaattaatc tttaaagaga ttttgggggt
     1021 gacctatgag gtaacttctg acaaaacgat tttcataaat tggaaaggtc catccattag
     1081 ctattgcagt aaacccgttc gggatgaaat attctttttt gctaccgaat tacttttcga
     1141 aaacggtata gtagtccaag aacctggatt tttaacttgg ggtggaacaa aagtctttta
     1201 tccaaccgtt cgcaactctg cattaccatt tgatcctttt gctgcatcct tttttcttac
     1261 gagtagatac gaggaatatc ttcctcatat tcgtgatcaa cacggtcgtt tcgagtccaa
     1321 acaaagtcta gcttatttac acggatttct tcaaaaacca gttgtcaatc tttgggcttt
     1381 agcgatttta caaatgatcg aagatcgtta tgggaaacaa acaatcaagg atcgtaacta
     1441 tagcttcaaa ccaacaattg atattgataa tgcttgggca ttccgacata aaggttttat
     1501 acgagcaggt ggaggaatgt tgtcggattt atttaaaatg aacctaaagg aatttgggga
     1561 gcgatttcga tctgttttcc atcttcagca cgacccatac gatacttacg agtatcaaat
     1621 tgagctaata caacgttaca agttaaatcc tttgtttttt attcttttgg gtgattatgc
     1681 cacattcgac aagaatgtac cacatcagaa taaacacatg caatccttaa ttaagttcat
     1741 gggtgatttt ggagatgtag gtatccatcc ttcctatgca tctaacgatc aacctgagaa
     1801 gcttccgaaa gaaattgctc gtttagcgta tattcttcat caggaaattc ataatagccg
     1861 tcaacatttc ttaagattga gtttacctga tacctatcaa cgccttatcg aatgtgatat
     1921 taccgacgat tatacgatgg gctatcccga tcagcctggt tttagagcag gtatttgtac
     1981 gccttacttt ttttacgatc ttgacctaga aatagaaact gcattaagat tacatccatt
     2041 tgcgatcatg gatggaacat tgcgtgacta tatgaacata aaaccggaag acgcagccga
     2101 tattattaat ccattaattg acgaaatcaa aaaagtaaaa ggagagttta tatccgtctg
     2161 gcacaatgag tcctttgccg aaaatgagcg ctggaaagga tggcggaaag tgtatgagga
     2221 tatgattgag aaggctctcg cctaacaaat gaaaaatcta cccaacctta aatttttgcc
     2281 tcattcccta ataaataaag tgctttggga tcaggcaatg aaaaatagtt ctaatggatt
     2341 agtatatgct agcacttggt atttagatgc tgttacaaac gataattggg atgcacttgt
     2401 ttcggaagat tatagttggc tgatgccttt gcccatcaag caaaaagctg gtgtgaaata
     2461 tttaccaact cctttattca ttcaacaatt gggaatattt ggaccaggac catttacacc
     2521 agaactaacc gatcactttt taaaacatat tgccgcaaat atcaagctgg ttgaatatca
     2581 attaaattcc gaaaatctag ctccatcatc tgatattttc gaagtaaaat tgaggttaaa
     2641 ccatctgtta aatctgcctg aggacaaatc acttttgatc aagtcgtaca gtgaaaatac
     2701 ggttcgcaat atcaagaaag ccgaaaaagc ccaagtgcgt tttggtaatg cttcaatccg
     2761 tcaaatgatt aagcttttcc aggatcataa agaacatgag ctagagaact ggaaaactga
     2821 gaattacaat gtgttggata agttatataa tgcttgtatg ctacgccaat tcggacaatc
     2881 aattggtgta tataatgccc aaggtgattt aattgctggt gcatttataa ccgaatggaa
     2941 ggatagagca acatttattt tctcttgtaa cagtccagaa ggcaaagaat ctggtgctct
     3001 accagcttta attcatcact atttgacgaa tgcccctgaa aaaatcaacc ttttcgattt
     3061 cgaaggctct gaccaagaag gtcttgcacg attctacaag agttttggga gcattgaatc
     3121 gaactatgta catttgaaac ttaatcgcct gccattttat atgcgctggt ttaaacactg
     3181 agcaatgtcg cggattctta gtaacgaaaa ctcccttgtt aatgtattca tcagcgaatt
     3241 gcgcgatgag accatccaaa aagatagcat gcgttttcga cggaacctcg aacggctagg
     3301 tgaaattatc gcttacgaaa tcagcaagca tcttgaatat tcagagaaag aagtaacaac
     3361 tccactcgga attgcaaatg tgccggtctt aaaagatcag ccggtaattg ctaccatttt
     3421 gcgtgctgga cttcctgttc atcaaggtat gctcaactat ttcgatcaag caggaaacgc
     3481 gtttgtttcg gcctatcgta aacatcataa ggacaatact ttcgaaatta aagtcgagta
     3541 catgagtagt cccgatttga acgataaggt gctaatcatt tgtgatccga tgttagctac
     3601 aggtcagtcg atgttacttg cctacaaagc cttaattcaa cgaggaaaac cacgccatgt
     3661 tcatgtggtt ggattaattg ctagtgtgga aggtttgact tatgtgaaaa gtcatttccc
     3721 tgagaataca accttttgga tgggtgcagt agatgatgaa cttactgtta aatcctacat
     3781 cgttccaggt ttgggtgatg cgggtgattt agcatacgga accaaaattt aagttcttga
     3841 ataaagctta ggtctttcat gactgaagca gaacattcca tctaccggga aattccatga
     3901 caacagccat tcagtttgtg ctactttttc tgttatgcac cgtcaagttt gtggtgggtt
     3961 taccagctgc ttatgctgct ttcaatttgg gattcttgga acttactctt tttggaagtt
     4021 tagcaggatt aacaggcgtt tcggcattca tgttttttag cgattaccta tttcgctttt
     4081 gggatgcttt tcgaattcga tttttccctc ctaagaaaaa cgttaagcgt aaaattttct
     4141 ccaaaagcag cagattttac gtaaaggtta tccgcaaata tggattggct ggtatagcat
     4201 tcataacgcc taccatcatt tcaattccag taggaacatt acttgcccga agattatatc
     4261 ccaatcggaa aaaagtgttt ttgtatttat tagtatcagt tgaactatgg tcgctaacct
     4321 tttcggcaat actgcatttt ccagctttac ttcgtaataa ttaacatgga gattaaagat
     4381 ttatttttcg atctcgaccg taccatttgg gattacgaaa ccaatagccg cgaaactctc
     4441 gacgatctac atgagatgtt tttagaacga actgttcccg gtgataaaga cacatttgcc
     4501 cgaatctttc gtttcgaaaa ctccttgctg tgggatgctt ttactgcaaa caaaatcgat
     4561 aaagactatt tgcgtaagaa tcgattcctc aataccattc gccgcatggg attaagcgat
     4621 cctgaattgg ccattcgtat ggaggatgct tacctcgaag ttactcctgc caagaaaagg
     4681 ctcttgccag gcgcatctga aacactccaa gttttagcgc aaaaatatcg tctacatatc
     4741 atcaccaacg gttttgaaga tgttcagaat tttaagctga ttaattgtgg aattcgaaaa
     4801 tacttcaacg aagtcatcac cagcgatggt gctaaatctc gaaaaccaaa tcgagatata
     4861 ttcgattatt cacttcaact tacaggagca tctatcgatg aaagtattat gataggtgat
     4921 gatcccgaaa ttgatatagt aggtgcacaa aaagcaggtt ggcaggcaat tctggtaaat
     4981 accatgaatc taacacatac gctacaagta agagaagtag ctagttttcc agaattactt
     5041 aattatctat aattaaaaaa tcccgctcct gaatacagaa gcgggatcta ataaaattat
     5101 aaaaccgaaa ttatttcgct ggttcagctg attttgcgcc tttaccgcaa gattttgaag
     5161 catcttttga gcagcaagca ccttttttct cagttgctcc attgtgacca gaaccagttg
     5221 cactagaaga tccagtttta gatccttcac cttcgtgaca agatttagct gatttctcat
     5281 ctttagaaca gcaagatttc tctccttttt tctttttgtc tttgtccttg tctttatcaa
     5341 gtttcactac aaccacattt ccttgattca taacagaagc aagtggagca gcaatagcac
     5401 tgctagtaaa agctgcaagg caaagaattg cgaggatttt tttcattttt cagatgtgta
     5461 tttaacaaat gtacaaaagc tgtgtgctta attattcact taacgtggaa atttagcaat
     5521 tattgtgata ccacctttaa ctacttcacc caattttact tgaacttccg catccaaagg
     5581 cataaaaata tctactcgtg aaccaaattt gatgaatcca aattcacctc cctgcttagc
     5641 ctcatcacca actcgagcat aggtaacaat tctacgagct acagcaccag caatttgacg
     5701 taacaaaaca gaacgctttc cattctcaac tactaccgtt gttcgctcat tctcagtcga
     5761 cgatttaggg tgcgaggcaa ccaaatacaa tccttcgtga tacttgaaat atgtcaccgt
     5821 acccgaaatc gaataccgat tcacatgcac atttaagggc gacataaaaa ccgaaacttg
     5881 cttgcgtttc cctttgtagt actcaggttc atccacttct tcgatcgcaa caattttccc
     5941 atcggctgga caaagaaccc aatcatcgcc cagattttga gtgcggttgg gacttctaaa
     6001 aaattgaagt acgaggataa aaatgatgaa agatgcaaca cctgttagtt tctgagccaa
     6061 acaggcatct ggaaacaggg aaaacaagga aacattaatg attgcaagta caatcaacaa
     6121 ggttaataat atacgatggc cttcgcggtg aatggtcatg ttattcgttt agaatgaata
     6181 attccttgct cttcccaatg gaagaaggga ttttatacta caaatgcatg tattgcttct
     6241 ttgcaagcaa ttgctcttgt gtttcacgat ggtcaggatc gtctacacag caatcaactg
     6301 gacaaacagc ggcacattgt ggttcatcat ggaaaccaac acattcggta catttatcac
     6361 ttacaatgta gtaagtatcc atttgtacag gtttttgtgt ggctcctgct tgaaatacac
     6421 cgctagcagc attaacttcg ccgcttaagg aagttccgtc ggagaaacgc cattcggttc
     6481 caccttcgta aattgcatta tttggacact ccggttcaca tgcaccacaa ttgatgcatt
     6541 catcagtaat catgatcgcc atttcacgtc agatttaagg gttattttcg cagcgcaaaa
     6601 ctaaggcata attgcattca tacatgacat ccgaacaacg catttcagca tttattcaac
     6661 ttggcaccga gatttatcaa actttccatg ctccagaaac ggaactaaac atacgccagc
     6721 agcagctttt acagctttcc gaccgcatgt atcacagcaa tggatggttc acattcgaaa
     6781 acaccaaaca tgcacttcta ggcatttctt ttatgctcaa ggaggaaaat attcgtcctt
     6841 ggtggaatca atatgctgat caatttcctc aagaaaaact tcatgctgtg ggtgttatca
     6901 tggctggaaa cattcccgct gtt
//
LOCUS       DAOOQW010000122         6781 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_13749_length_6781_cov_5.549658, whole genome shotgun sequence.
ACCESSION   DAOOQW010000122 DAOOQW010000000
VERSION     DAOOQW010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 6781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6781
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13749_length_6781_cov_5.549658"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2095)
                     /locus_tag="PK281_07665"
     CDS             complement(<1..2095)
                     /locus_tag="PK281_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008991476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 115 family protein"
                     /protein_id="HOY49262.1"
                     /translation="MSFLFCLIFVSTVLLGQENYVSKIGGKGFFNLSSSGKSTSMWIS
                     ATEFPGVVRAAKDLQEDIAKVTGAKPVLLTGKSEGREAVIIGTIGKSELIDELIKNKK
                     INVTSIQGKWETSITQIVEKPMKGVDRALVIVGSDKRGTIYGIYELSKQIGVSPWHWW
                     ADVPAKKSTSLFVLPGQHTLGEPAVKYRGIFLNDEEPALGRWAVEKYGGFNHECYETI
                     FELILRMKGNYLWPAMWWASFNTDDPLNQKLADEYGIVMSTTHHEPMMRAHADWKAFK
                     GSAGEWNYDKNQETLKKFWREGIERMGNYESIISMGMRGDGDMAMEEDTNISLLERIV
                     KDQREIITDVTKKPLNQTPQLWALYKEVQDYYDKGMRAPDDVTLLLCDDNWGNNRKLP
                     KLEDPKRSGGYGIYYHFDYVGGPRNYKWLNTNQISRTWEQMHLAYEYGADRIWIVNVG
                     DLKPMEFPIEFFLDYAWNPKRWTVDNLGDYTRMWAEEQFGGEHAAEIANILTKYTTYN
                     ARRKHELLSPETYSLINYREAETVTKDYQQLTIEAERIYALMPAEYKDAYYQLVLHPT
                     KASANLYALYFAVAKNRLYATQGRAETNSLAEEARQLFKKDSLLSHYYNTVMANGKWN
                     HMMDQTHISYTYWQQPAKDVLPEVKTITLPVPAEMGVAIEGFVHWETPTSKIGSNDKI
                     EFPLLRYDCFNQQSQY"
     gene            complement(2182..4926)
                     /gene="galB"
                     /locus_tag="PK281_07670"
     CDS             complement(2182..4926)
                     /gene="galB"
                     /locus_tag="PK281_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008992100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase GalB"
                     /protein_id="HOY49263.1"
                     /translation="MTRIILSGFKYCGLLYLVAFTNTMVLAQSSGSTNSRQRISINEG
                     WYFYKYDSITKADNLIYDVRPAIINNVENKVADAKPTEAVKVESVSDVLKPWILPSGN
                     RFIKDADNKHVRPRGNPGGDFQFVKNDFADNHWKKINLPHDWSINGPFMSGQNPEVGG
                     GMGRLPSNGIAWYRKKIEIPASDKGKSIFLDVDGAMSYAMVWLNGNLVGGWPFGYGSW
                     RLDLTPYIVPGGDNQLAIRLDNPNNSSRWYSGGGIYRNVWLTKTNPIHVGQWGTYITT
                     KNISKASATIDLEVMIDNDSKTKATIEAVTRIYELDARGNKSEKVIGSFASLTTGIES
                     EGSVKLQSSLTIKNPKLWGPPPTQQPNLYVAQTTLMQNGKPIDTYETRFGIRSLEFNP
                     DKGILVNGERIQVKGVNQHHDLGALGAAFNTRAAERQLEMLREMGCNAIRMSHNPPAP
                     ELLELTDRMGFLVVDEIFDSWERKKTPHDFHLIFPEWSEPDARAFIRRDRNSASVIMW
                     SIGNEVGEQYNGEEGAAVARRLHNFFNEEDPTRPTTTAMNFAKPDMPLPQVVDVIGLN
                     YQGEGIRQTPEFEGTERIRTPPQYDAFHKQFPDKVILSSETASAFSSRGIYLFPVSKE
                     VSAPVRDGRGGNSKIHQVSSYELHAVDFGSSAEKVFASVERHPFVAGEFVWTGWDHLG
                     EPTPYYDSRSAYCGIIDLAGFKKDRFYLYQSHWRPEFPMAHILPHWNWAERVGEITPI
                     HVFTSGDEAELFLNGKSLGRKKIAQYEYRLRWDSVKYEPGEVKVVAYKKRKLWAEDIV
                     KTTDQPTKLIVSADRESIQADGNDLVFITVQIADKDGLMVPRSNNNIVFSIEGPGEIV
                     ATDNGDPTNMHSFSSHNREAFNGLCLVIVRSVKGKRGNITLTAKSTGMKNCQVNIKSN
                     "
     gene            complement(5294..>6781)
                     /locus_tag="PK281_07675"
     CDS             complement(5294..>6781)
                     /locus_tag="PK281_07675"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOY49264.1"
                     /translation="YHTDITYQIANGNSNFNTEFGPQSGWNNHWGWLYKIVSEANTVL
                     SRIPSSQLSDADKVAFEAKAKFFRAFGYRNLAYLFGGVPLITEEITTEKVDFVRATRQ
                     EVYAQIISDLTFCTLNLPAITAVRDGEVSNLAAQHLLAEVYLADGQFQKAVDAATVVI
                     DDANTDLMVTRYGSRVSVVPGDVYWDMFQAKNQNRITANNKEAIWVIQIETDVLGGSA
                     VSSAQAGSFLLERVHPPLIRDLRVNGVAPFNWPVGDYSGGRGVGFMAPSQYFINNAYD
                     DPDNDIRNANHNFVRKYLANNPNSPLNGQLIDFANMPANSTGTNGSVITSGNADRSIY
                     PYQTKSTQPFNHPAALYDSRKQFPYQLTGAAGGTYADQYMFRLAETYLIRAEAYLGLN
                     NTALAADDINVVRARSNAAPVAPANVDIDYILDERMRELGVEEKRLLTLTRLGKVFDR
                     IKKCNPYYDGANGGSAVQAHNNLIAIPQAQIERNRGAVLTQNPGY"
BASE COUNT         1890 a         1511 c         1234 g         2146 t
ORIGIN      
        1 tgtattgact ttgctgattg aagcaatcat agcgtagcag aggaaattcg attttgtcat
       61 ttgatccgat tttggaagtt ggcgtttccc aatgtacaaa accctcaata gcaactccca
      121 tctcagcagg tactggtaag gtaattgttt ttacttcagg aagaacatcc tttgcgggtt
      181 gttgccaata ggtataactg atatgcgtct gatccatcat gtgattccat tttccattgg
      241 ccattaccgt attgtagtaa tgggaaagca gagagtcttt cttgaagagt tgtctggctt
      301 cttcggctag cgaattggtt tccgctctac cttgcgtggc atacaatctg tttttcgcaa
      361 cagcaaaata aagtgcatac agattggcac ttgcttttgt aggatgtaag accagttgat
      421 agtaagcatc tttatactcc gcaggcatta atgcataaat tctttctgct tcaatggtaa
      481 gttgctgata gtccttcgtt accgtttctg cctcacgata attgataagg ctataagttt
      541 caggtgataa taattcatgc ttccttctcg cattataggt tgtgtacttc gttaagatat
      601 ttgcaatctc agctgcgtgc tcacctccaa attgttcttc agcccacatg cgggtgtagt
      661 caccaaggtt atctaccgtc cagcgttttg gattccaggc gtaatcaaga aaaaattcaa
      721 tggggaattc catcggcttt agatctccga catttacgat ccagatgcga tcagcaccgt
      781 attcataggc aagatgcatc tgctcccacg tgcgagaaat ctgattggta tttaaccatt
      841 tataatttct aggaccacct acataatcga agtgataata aattccataa ccgccactgc
      901 gcttaggatc ctcaagcttt ggaagcttac gattgtttcc ccagttgtcg tcacacaata
      961 ataaagttac atcatcagga gcacgcattc ctttatcgta ataatcctgt acctctttgt
     1021 agagtgccca cagttgaggt gtttgattca atggcttctt cgttacatca gtgattatct
     1081 cacgttgatc ttttacaatt ctttccagca gcgaaatatt ggtatcttct tccatcgcca
     1141 tgtccccatc tccacgcata cccatagaaa taatactttc gtaatttccc atgcgttcaa
     1201 ttccttccct ccagaatttc tttaacgttt cttggttctt gtcgtagttc cattcaccag
     1261 ccgatccttt gaatgctttc cagtccgcat gagcgcgcat catcggttca tgatgtgtag
     1321 tgctcatcac gattccatat tcatcggcta gtttttgatt caacggatca tcggtattga
     1381 aactggccca ccacatggct ggccataggt aattaccttt cattcgtaag atcaattcaa
     1441 aaatggtttc gtaacattcg tgattgaacc caccatattt ttctacagcc catctcccca
     1501 acgcgggctc ttcatcgttc agaaaaattc cacgatactt taccgctggt tcacctaagg
     1561 tatgttgccc gggcaaaaca aagagacttg tacttttctt agccggtaca tcagcccacc
     1621 aatgccaggg agaaactccg atttgtttgg agagttcata aattccatag atcgttccgc
     1681 gtttgtcgct cccaacaatc accaatgcgc gatcaacacc tttcatcggt ttctcaacaa
     1741 tctgtgtaat cgatgtttcc catttacctt gtatggatgt tacattgatt tttttattct
     1801 taatgagttc atcaatcaat tcactttttc caattgttcc aatgatgacg gcttcacgtc
     1861 cttcagattt acctgttagc aatacaggtt tagcacctgt tactttagca atatcttcct
     1921 gaagatcttt tgcagcgcga accacaccag gaaattctgt tgcgctaatc cacatcgatg
     1981 ttgatttacc cgaagaggaa agattaaaga atccctttcc cccaattttg gaaacgtaat
     2041 tttcttgtcc taaaagaaca gtagaaacga aaatcagaca aaaaagaaat gacatttttt
     2101 gaattgtccc agtgcaattt gttgatggta cattactatg catgttgtcg ttatttattg
     2161 aataaaataa ttccttagtc actaattact tttaatattt acctgacaat ttttcatacc
     2221 cgttgattta gctgtcaaag tgatgttccc cctttttcct tttaccgaac gtacaatcac
     2281 caaacaaagt ccgttgaatg cttcgcgatt gtgagaagag aaagaatgca tgtttgttgg
     2341 atcaccattg tcggtcgcta caatttcacc aggaccttca atgctaaaca caatgttgtt
     2401 gttagaacga ggtaccatga gtccatcttt atctgcaatc tgcacggtaa taaaaactaa
     2461 atcatttcca tcggcctgaa tactttcgcg atctgctgaa acaattagct tcgttggttg
     2521 atccgttgtc ttgacaatat cctcagccca caattttctt tttttgtatg caactacttt
     2581 cacttcaccg ggttcatact tcacactatc ccagcgcaga cgatattcat attgcgctat
     2641 ctttttccta cctaatgatt taccgttgag aaatagttct gcttcatcac ctgaagtaaa
     2701 aacatgtatg ggcgtaatct ctccaactcg ttcagcccaa ttccagtgtg gaagaatatg
     2761 tgccatagga aactctggtc gccagtgtga ttgatatagg tagaagcgat ctttcttaaa
     2821 acctgctaaa tcaataatac cacaatacgc actacgtgaa tcgtagtaag gcgttggttc
     2881 acctaaatga tcccaacccg tccagacaaa ttctcctgca acaaaaggat gtcgctctac
     2941 tgaagcaaaa actttttcag cggatgaacc gaaatcaaca gcatgaagtt cgtatgaact
     3001 tacctgatga atctttgagt taccacctcg tccatcgcgc actggtgcac ttacttcttt
     3061 cgaaactgga aataagtaaa tacctctgct actaaatgct gatgctgttt cactgctgag
     3121 aattacctta tcgggaaatt gtttatgaaa cgcatcgtat tgtggtggag tccgaatgcg
     3181 ttcggttcct tcaaactctg gtgtttggcg aataccttcg ccttgataat ttaatccgat
     3241 aacatcaaca acctgaggta acggcatatc cggttttgca aagttcatcg ctgttgtggt
     3301 gggacgggtg ggatcttctt cattaaaaaa gttatgcaaa cgtcttgcga cagcagcacc
     3361 ttcttcacca ttgtattgtt cgcctacttc attaccaata ctccacatga tcacagaagc
     3421 actgttccta tcccttcgaa taaatgcacg agcatcaggt tccgaccact ctggaaatat
     3481 caaatgaaaa tcgtgtggtg ttttctttcg ttcccaggaa tcaaatattt catcaactac
     3541 taagaagccc atacgatccg ttaattcgag caactcgggt gcaggaggat tatgagacat
     3601 gcgaatagca ttgcatccca tctcacgaag catttccaat tgacgttcgg cagcgcgtgt
     3661 gttgaaagct gcacccaacg cacctaaatc atgatgttgg ttaacacctt ttacttgaat
     3721 gcgttccccg ttaaccaata ttcctttatc cggattaaat tctagcgagc gaataccaaa
     3781 acgtgtttcg taagtatcga tgggttttcc attttgcatc aaggttgttt gagctacata
     3841 aagattcggt tgttgtgttg gaggcggacc ccatagttta ggatttttaa ttgttagaga
     3901 actttgaagc ttcacgcttc cttccgattc aatacctgtt gttaagcttg caaaacttcc
     3961 gatcactttt tcggatttgt tgcctctggc atcaagttca taaatgcgcg ttacagcttc
     4021 aatggttgct tttgtcttcg aatcattatc aatcattact tcaagatcaa ttgttgcaga
     4081 tgctttcgaa atattttttg tggttatata ggtaccccat tgcccaacat gaataggatt
     4141 ggtttttgtt aaccacacat tacggtaaat accaccaccc gaataccagc gagatgagtt
     4201 attaggatta tccagtcgaa tggccaattg attatcacca ccaggcacaa tgtaaggagt
     4261 aagatcaagc ctccacgaac cgtaaccaaa aggccaacca cccaccagat ttccattcaa
     4321 ccataccatt gcataggaca ttgcaccatc aacatccaaa aaaatagatt tgcctttatc
     4381 gcttgcaggg atttctattt tttttctgta ccaggctatg ccattgctgg gtagtcttcc
     4441 catccctcca cccacttcag gattttggcc tgacataaat ggtccattga tagaccagtc
     4501 gtgtggtaaa tttattttct tccagtgatt atcggcaaaa tcatttttta caaattgaaa
     4561 gtcgcctccc ggatttcctc ttggtcgcac atgtttattg tcagcatcct ttataaagcg
     4621 atttcctgaa ggtaatatcc acggcttcaa tacgtcactc accgattcaa cttttacggc
     4681 ctcagtgggt tttgcatccg caaccttatt ttcaacattg ttaataattg caggacgaac
     4741 atcgtaaatc aagttgtcgg cttttgttat tgagtcgtat ttataaaaat accaaccttc
     4801 gttaatagaa atgcgctgac gactattggt acttccagaa ctttgtgcca gcaccatagt
     4861 atttgtaaac gctaccagat atagtaaccc gcaatactta aatccactta aaataattct
     4921 tgtcatttat ggagtatgat ttttcttgaa cgcttagaat ccagctacgt gatcaatttg
     4981 agttagttaa aatacacaaa ctaatacaca gttcctgcaa cgaaaattaa aagtatcaat
     5041 ccgtttttga aaccggttcc actaagggat aataggtcga aatcagagag cttcttgaaa
     5101 ttaaactaaa gccttcaccc tctcaggaag ctttgttaaa agtttacatt aaaaagatga
     5161 gtgcctagaa aatgtgcttt acaaaattga atggtttata aagaaaaaga agaggggatt
     5221 tacatccacc tctccttcgt tctctaaacc caaataattt tcaagaatta ataataaatc
     5281 tgaatttggt tttttaatag ccagggtttt gtgttaacac agctcctctg tttctttcta
     5341 tttgtgcctg tggaattgct atcaaattat tgtgagcctg aacggcactt ccaccattgg
     5401 caccatcata gtatggatta cacttcttga tcctgtcaaa aaccttacct aacctggtaa
     5461 gtgttagtag tctcttttct tcaacaccaa gttctctcat tctttcatca agaatataat
     5521 caatatcaac attcgcagga gctacaggag ctgcatttga tcttgctctc actacgttaa
     5581 tgtcatcagc agctaatgca gtgttattaa gaccaagata tgcttcagct cgtatcaaat
     5641 aggtctctgc taatctaaac atatactgat cagcataggt acctcctgcg gctcccgtta
     5701 actgataagg aaattgtttt cgactatcgt ataacgcagc ggggtgatta aaaggttgag
     5761 tagattttgt ctgataggga taaatacttc tatccgcatt tcctgatgtt ataaccgatc
     5821 catttgttcc tgtagaattg gcaggcatat tcgcaaaatc aataagctgt ccattgagag
     5881 ggctattcgg attgttcgct aaatattttc ttacaaaatt atggttggca tttctaatat
     5941 cattatcagg atcatcataa gcattgttga tgaagtattg agatggtgcc ataaagccaa
     6001 cacctctacc tccagaataa tcgcctacag gccaattgaa aggcgctact ccatttactc
     6061 tcaaatcacg aatcagagga ggatgaactc gttcaagcaa aaatgatccg gcctgagcag
     6121 aggaaacagc tgacccacca agaacatcgg tctcaatttg tataacccaa attgcttctt
     6181 tgttgttggc cgttattcta ttttggtttt tggcttgaaa catatcccaa taaacatctc
     6241 ctggcactac actcactctg gatccatatc ttgttaccat caagtctgta ttggcatcat
     6301 cgataacaac agtagctgca tcgactgcct tctgaaactg accatcggcc agataaactt
     6361 cagcaagtaa atgctgtgcc gctaagtttg atacctcacc atcgcgtact gcggttattg
     6421 ccggaagatt tagggtacag aaagttaaat ctgaaattat ttgagcatac acttcttgtc
     6481 tggttgctct gacaaagtct actttttctg ttgtaatttc ttctgtgatc aagggaaccc
     6541 caccaaaaag ataagcgagg ttgcgatacc caaaggctct gaagaattta gcttttgcct
     6601 caaaggctac cttatcggca tcgcttaatt gcgaacttgg aattctactc agaacagtgt
     6661 tggcttccga aacaatcttg taaagccaac cccagtgatt gttccacccg gactgtggtc
     6721 caaattcagt attgaaattt gaattcccat tggcaatctg ataggtaatg tccgtatggt
     6781 a
//
LOCUS       DAOOQW010000123         6770 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_13773_length_6770_cov_4.765302, whole genome shotgun sequence.
ACCESSION   DAOOQW010000123 DAOOQW010000000
VERSION     DAOOQW010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 6770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6770
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13773_length_6770_cov_4.765302"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1871
                     /locus_tag="PK281_07680"
     CDS             <1..1871
                     /locus_tag="PK281_07680"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY49265.1"
                     /translation="APDIFNGDLVLENSGSDVINLAYGSTGNQFNGNIELNSVGSSSG
                     IRFGQNAGTSTLASSKTLSIGGSGFTSGDLRFAYFTQTGSTSQSLTTFGSGVELYLSE
                     GTVFNGTTTFTCDNIYLDGTTFNSNTTITKTGNGFNLSNGGNIFNGSTIISNTGTGSF
                     ILAGSSSDTFNDNVQFLQSTSFTLYPAYNVNSNFKKNISTVGSTTAIVFASNGGRVTM
                     NGTVAQSIEGDAAQLPDFFNLTINKSSNHVTLNVPILVTNNLTLTNGNLVSSGTNLIT
                     MVDNSTVSSVSNSSYVSGPIRKEGNDAFTFPIGKSGYYRPIGISAPSSTGDHFTAEFN
                     MADSDGSYSHASKDGSLHHLSHCEYWILNRTNGSSSVSVTMSWNTSSCGVTNLSDLRV
                     ARWNGSMWKDHGNGGTTGNTSVGTIVSSGTISSFSPFTLSSNTNENPLPVHLVHFGAT
                     PLNSDVVLEWTTVSESNSDYFSIESSTDAVNFKEIGRVSAAGNSNTTLNYRSVDSSPH
                     AGVSYYRLRQVDFDGKFMFSNIEVVNMATLWDSEVVMSPNPVINTVDIRLDPDNFLNP
                     ILEIHDMQGRLIMVKSDITVDAQKPVRMDLSTLPHGLYFLQISENGKSISKRLIKD"
     gene            complement(1921..3327)
                     /gene="lpdA"
                     /locus_tag="PK281_07685"
     CDS             complement(1921..3327)
                     /gene="lpdA"
                     /locus_tag="PK281_07685"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014938447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HOY49266.1"
                     /translation="MDSFDVAIIGSGPGGYVAAIRCAQLGMKTALIERYATLGGTCLN
                     VGCIPSKAMLDSSEHFYNAGHTFAEHGIQLSNLKADLGQMVKRKNEVVAQTVAGIDFL
                     MKKNKITVLRGHGSFINSTTIRISKDDKSSQDIQAKNIILATGSKPTSVPGVEIDKKR
                     IITSTEALNLKEIPKHLIIIGGGVIGMEMGSVYARLGAEVSVVEYTDSICGAFDGTMR
                     KELQKVLSKLGFKFYLQHKVTSAVSKGKSVSVTAEALKDGAKLELKGDYVLVCTGRRP
                     YTDSLGLENAGLAVDEKGRVPVNENCQTSVSNIYAIGDLVRGAMLAHKAEEEGTFVAE
                     FLAGQKPHINHLLIPNVVYTWPEVASVGYTEEQLKADKRTYKVGSFPFKASGRARASM
                     DTDGVVKMLTDADTDEILGVHIIGPRAADMIAEAVVAMEFRASAEDIARISHAHPTFT
                     ESVREAALAATGNRAIHM"
     gene            complement(3381..4991)
                     /gene="lnt"
                     /locus_tag="PK281_07690"
     CDS             complement(3381..4991)
                     /gene="lnt"
                     /locus_tag="PK281_07690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HOY49267.1"
                     /translation="MKNTFRLLLLALLSGILLMFSWPEIGFSPLIFIALVPLLSIEET
                     LLQSGKARSNLAVFGYAYLSFLIWNLGDTWWVKNASFEGALMAFFANSALMAGTFTLF
                     HRTRKVLRSPNSIYYLPVFWLAFEYIHHVWSISWPWLTFGNVFANSVQLIQWYEFTGT
                     SGGSLWVILINCAAFNVLRTWIQDKEAPIKPAVRRLLFGLLIPSLVSVALYFYRSEDR
                     GEIARVAIIQPNVDPYNEKFDGMSLDEQTNKILRLAKKVTQPQTDWLICPETALVGSM
                     DESSLSQQPRIELFKQLTDTFPRLNVLIGAETHQVYMPGQKTVTARKARGGDVYYDAF
                     NTAVMINSRGVRTYHKSKLVPGVEIMPFPWLFKHIESFAIDLGGTTGTLGMQEERSVF
                     SGVDAKQKVAPAVCYESVYGDFMTGYMDNGANFIAIITNDGWWGETPGYKQHLAYARL
                     RAIETRKSIVRSANTGVSCFINQRGDILQAQPYWQEAAIEGEIKLNSERTIYSYTGDL
                     FGLSACFLAIWLYIWSFYLRFKQRRNKA"
     gene            5392..>6770
                     /locus_tag="PK281_07695"
     CDS             5392..>6770
                     /locus_tag="PK281_07695"
                     /inference="COORDINATES: protein motif:HMM:NF013604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HOY49268.1"
                     /translation="MKLTRYLIILTLLFLIQVSSIWAQTYIKGVDAEKMINGSSKILV
                     NEKLKTPQFIDLKIPVQQDPTTWLKTALQLPATDDFRPIDTIKADKLGWIRYKYQQYH
                     EGYEVELGQYSVFVQQDKIIRAAGAFLMPIEISNASIIDASQCLKSATQQLESEGIKI
                     ENLTGNTSFEEKPKIVLDKGIYHLCYEFEFYSIEPVSKKGVLVDVHTGQVIQVRDRII
                     CTDVVGTANTMYYGTKSITANMVSAGNYQLKDNGRGITTWDLNGSSSSSSAVYFTNTT
                     NNWTSTVNQDDAAYTCHWALEGTYDYYFNTQALDSYDDLGAEINAYVHYSTSYTNAFW
                     DGSGLYFGDGGTGYDPLTSVDIVAHEFTHAVTEYSAGLIYSYESGALNESYSDIFGVV
                     VDYELNADANFLIGDVSSETGTPFRNMSDPNITSQPDTYEGTYWNSSGSDNGGVHTNS
                     QVMNYWFYL"
BASE COUNT         2090 a         1399 c         1292 g         1989 t
ORIGIN      
        1 cagctcccga tatcttcaat ggagatttag tattggagaa ctcaggctcg gatgtaatca
       61 atcttgctta tggttcaaca ggaaaccaat tcaacggaaa tatcgaactc aatagtgtag
      121 gttcttcttc tggaatccgt tttggtcaaa atgctggaac ctcaacacta gcaagctcta
      181 aaactttaag tattggtggt tcaggattta catctggtga tttacgtttt gcttatttca
      241 ctcaaacagg ttcaaccagt caatctctaa ccacatttgg aagcggtgtt gaactttacc
      301 tttccgaagg aacagttttc aacggcacaa cgactttcac gtgtgacaat atctaccttg
      361 atggtacaac attcaattcc aatactacca tcacaaaaac cggaaatgga tttaaccttt
      421 ctaacggagg aaatatattc aacggctcaa ccatcatctc caatacagga acaggttcgt
      481 tcattttagc tggttcttcc agtgatacat tcaacgataa cgtgcagttt ctgcaatcga
      541 cttcatttac actgtatcct gcatataatg taaactcaaa tttcaaaaag aatatctcta
      601 cggttggctc tacaacagct atcgtttttg ctagcaatgg gggacgagta actatgaatg
      661 gaactgttgc tcaaagtatc gaaggcgatg cggctcaact tcccgatttc tttaatctca
      721 caatcaacaa aagttcgaat cacgtaacct tgaatgttcc aattttggta acaaacaatc
      781 ttactttaac caatggaaac cttgttagta gcggaactaa tttaatcacc atggttgata
      841 attcaaccgt atcgagtgtg tcgaatagca gctacgtttc tggacctatc cggaaagaag
      901 gtaatgatgc ctttactttc ccaatcggaa aaagcggata ctatcgtcca atcggaatct
      961 ctgctccttc ttcaaccgga gatcatttta cagctgagtt taatatggcc gattcggatg
     1021 gtagctactc tcatgcttct aaagatggtt ctctccatca cttaagtcat tgcgaatact
     1081 ggattctaaa tcgtaccaat ggttcatcga gtgtttcagt aacgatgagt tggaatacat
     1141 ccagctgtgg agttactaac ctcagcgatt tacgtgttgc acgttggaat ggcagcatgt
     1201 ggaaagatca tggaaacggt ggaacaactg gtaacactag cgttgggaca attgtttcta
     1261 gcggtacgat tagcagcttt agtccattta cactttcttc taataccaat gaaaatccac
     1321 ttcctgttca tcttgttcac tttggtgcaa caccattaaa cagcgatgta gttcttgaat
     1381 ggactaccgt ttcggaaagc aatagcgatt attttagcat tgaatcaagt accgatgctg
     1441 ttaacttcaa agaaattgga cgtgtatcag ctgctggaaa tagcaacact acactcaatt
     1501 accgcagtgt tgattcttca cctcatgctg gcgtttcata ctatcgttta cgtcaggtcg
     1561 acttcgatgg taaatttatg ttctccaata tcgaggtagt taatatggcc accttgtggg
     1621 attcggaagt agtgatgagt ccaaatccag taattaacac ggttgacatt cgcctagatc
     1681 ccgataattt cctaaaccca attctcgaaa ttcacgacat gcaaggtcgc ttgataatgg
     1741 ttaaatcgga catcacagta gatgctcaaa aaccagtaag aatggatctt tccacattac
     1801 cacatggctt gtacttttta caaatcagtg aaaatggaaa gtcaattagc aagcgattga
     1861 taaaagacta aatcaaacca cctcaaataa aaaagggagc tcgaaaggct ccctttttta
     1921 ttacatgtgt atcgctcgat ttcccgtggc tgccaatgct gcttccctaa ccgattcggt
     1981 aaaggttgga tgtgcatggc taattcgggc aatgtcttcg gccgatgctc tgaactccat
     2041 agcaacaaca gcttccgcaa tcatatccgc cgcgcgtgga ccaataatat gtacgcctaa
     2101 aatttcatct gtgtcggcat cggttaacat cttaacaaca ccgtctgtat ccatggatgc
     2161 acgagctctt cctgatgctt tgaaaggaaa acttccaact ttgtatgtac gcttatcggc
     2221 ttttaattgc tcttcggtat aacctacact ggctacttct ggccaagtat aaaccacgtt
     2281 aggaatcaat aagtgattga tgtgaggttt ttgtccagct aagaattctg ctacaaacgt
     2341 tccttcttct tcagccttgt gtgccaacat cgctccgcga actaaatctc cgattgcata
     2401 gatgtttgaa acactcgtct ggcaattttc atttaccgga acgcgacctt tttcatccac
     2461 tgccaatcct gcattttcta gtccgagtga atcggtataa ggacgacgac ctgtgcaaac
     2521 taatacgtaa tcacctttga gttcgagctt agcaccatct ttcaaggctt ctgcagtaac
     2581 agaaaccgat ttgcctttgc taacagcaga agttaccttg tgttgtaagt agaatttaaa
     2641 tccaagtttg ctgagcacct tttgcaattc cttgcgcata gtaccatcaa aagcaccgca
     2701 tatcgaatcg gtatattcta caaccgaaac ttcagcacct aaacgtgcat ataccgaacc
     2761 catttccatc ccgataactc cgccaccaat gatgattaaa tgcttcggaa tctcttttag
     2821 attaagtgct tcggttgatg tgatgattcg ttttttatca atctcaactc cgggaacgga
     2881 agtcggttta gaacctgtag caaggatgat attcttcgcc tgaatatcct gacttgactt
     2941 atcgtctttg cttattcgga tagtagttga attgataaat gaaccatgac cacgtaaaac
     3001 cgtaatcttg tttttcttca tcaggaaatc aatacctgca acggtttgcg caactacttc
     3061 gtttttccgt ttcaccatct gccccaaatc tgctttcaag ttagaaagct gaattccgtg
     3121 ttcggcgaat gtatgtccgg cattgtagaa atgttccgaa gaatcgagca ttgctttaga
     3181 tggaatgcaa ccaacattca aacatgttcc gccaagagta gcgtatcgct cgatgagagc
     3241 tgtcttcatt cccaattggg cacatcgaat agccgctaca tatccgccag ggcccgaccc
     3301 aattattgca acatcaaaag aatccatgta aaattttgtt ttaatcaaca gaggcgcaaa
     3361 agtacaaaag gcaaaggcaa tcaggcttta tttctacgct gcttgaatct taaatagaat
     3421 gaccagatat ataaccaaat agccaagaaa caagctgaaa gtccaaacaa atcacccgtg
     3481 taagagtaaa ttgttcgttc ggaattgagt tttatttctc cctcaattgc cgcttcttgc
     3541 cagtatggtt gagcttgaag aatatcacca cgttgattga taaaacaact cacgccagta
     3601 ttggcagatc gcacaatact ttttcgcgtt tcaatagcac gcaaacgagc ataggctaaa
     3661 tgttgcttgt agcccggagt ttcgccccac caaccatcgt tcgtaataat ggcaataaag
     3721 ttggctccgt tatccatgta gcctgtcata aaatcaccgt aaaccgattc gtaacacaca
     3781 gctggcgcga ctttctgctt ggcatcaaca cccgaaaaca cacttcgctc ttcttgcatt
     3841 cccaaggtgc cagttgttcc acctaaatca atcgcaaacg attcaatatg cttgaataac
     3901 catggaaaag gcatgatttc cacacccgga actaatttcg atttatgata cgttcgcact
     3961 ccacgtgaat taatcatcac tgcggtatta aacgcatcgt aataaacatc tccgccacgt
     4021 gcttttcggg cagttacggt tttttgtccg ggcatataaa cctgatgcgt ttcggcacca
     4081 atcaacacgt tcaaccgggg aaatgtatcc gttagttgtt tgaacaattc aattcgaggt
     4141 tgctggctta aagaagactc atccatcgag ccaaccaaag ccgtttctgg acaaatcaac
     4201 caatcggttt gaggctgagt gactttttta gcgaggcgaa gaattttgtt ggtttgttca
     4261 tctagggaca tcccatcgaa tttctcattg taaggatcaa catttggttg aatgatagct
     4321 acacgagcta tttcccctcg gtcttcggaa cgataaaaat acaaggcaac cgaaacaaga
     4381 gaaggaatga gcaatccaaa taataatcta cgaacggcag gttttatggg cgcttcttta
     4441 tcttgaatcc aagttcgtag tacgttaaat gcggcacaat taattaagat tacccaaagc
     4501 gaaccacccg aagtaccagt gaattcgtac cattgaatga gttggactga attggcgaat
     4561 acgtttccga atgtgagcca tggccatgaa atactccata catgatggat gtattcgaat
     4621 gccaaccaga aaacgggtaa ataataaatt gaattcggtg agcgtaatac tttacgagtg
     4681 cggtgaaaca aggtaaaagt tccagccatc agtgctgaat tggcaaaaaa tgccataagt
     4741 gcaccttcga aagaagcgtt tttcacccac caagtatctc ccaaattcca aataagaaaa
     4801 ctcaaataag cgtatccgaa aacagctaga ttacttcttg cttttcccga ttgaagcagt
     4861 gtttcttcaa tacttaataa aggaactaaa gcgatgaaaa taaggggaga aaaaccaatc
     4921 tccggccagg aaaacattag tagaattccc gataagaggg ctaaaagtaa aaggcgaaac
     4981 gtattcttca ttggagccaa agataactta gtataaggct ataaaattta ataccttatc
     5041 aacttatagg ttagtttatg ctaattaaac ctcaacgaca gttctaatac agaaaatcga
     5101 attaaacaat cctggttgaa atcatcaact gttactcaat agcttgtttt tctggttgaa
     5161 tatggttagt gaagagatat aacctgttct tcgcatgaac tcatacacat catatactat
     5221 caattattac actcacatca gtataattgg tccgttcgaa aaagattgaa ataaaagtac
     5281 cacacattat ttttttagtg aaaaattaaa aagtatctgt ttttaaattc aatgtcttaa
     5341 attggtcgtt aacaaaacca gcgttacgaa aaatctatta atctaccaat tatgaaactt
     5401 actcgatatt taatcattct aaccctactg ttccttatac aagtcagctc gatttgggcg
     5461 caaacctata ttaaaggagt tgatgccgaa aagatgataa acggctcttc caaaatactg
     5521 gttaacgaga aactgaaaac accacagttt attgatttaa aaattccagt tcaacaagat
     5581 ccaacaacat ggttgaaaac ggcacttcag cttcctgcaa cagatgattt tcgtccgatt
     5641 gatacaatta aggctgataa gttaggttgg attcgataca aataccaaca atatcacgaa
     5701 gggtatgagg tagaattagg gcaatacagt gttttcgtac aacaagataa aatcattcgt
     5761 gcagcgggtg cttttttaat gccaattgaa ataagtaatg cttctattat agatgcgagt
     5821 caatgtttga aatctgcaac acaacaatta gaatcggaag gaattaaaat tgaaaattta
     5881 accggaaata cctcatttga agaaaaacca aagattgtat tagataaagg tatttatcac
     5941 ttgtgctatg agtttgaatt ttattcaatt gagccagttt ctaagaaagg tgttctagtt
     6001 gatgttcata ctggacaagt tatccaggtg cgtgatcgca tcatttgtac tgatgttgta
     6061 ggaactgcga atactatgta ttatggcaca aagtcgataa cagccaatat ggtttctgca
     6121 ggcaattatc aactaaaaga taatggcaga ggtatcacca catgggattt aaatggttct
     6181 tcaagttctt ccagcgcagt atattttacc aatacaacta ataattggac ttcaacagtc
     6241 aatcaagatg atgctgcata tacatgtcat tgggctcttg aagggactta tgattattat
     6301 ttcaacactc aggcccttga ttcttatgat gatttagggg cagaaattaa cgcttatgtt
     6361 cattactcaa caagttatac caatgcattt tgggatggtt cggggctata ttttggagat
     6421 ggagggactg gttatgatcc gcttacgagt gtagatattg ttgctcatga atttacacat
     6481 gctgttacag aatatagtgc tggattgatt tattcatacg aaagtggggc acttaatgag
     6541 agctactccg acatttttgg tgttgttgta gattatgaat taaatgcaga tgcaaatttc
     6601 ctaataggag atgtaagtag cgaaaccggc actccattcc gcaatatgtc tgaccccaat
     6661 attactagtc aaccagacac gtatgaagga acatattgga atagttcagg gtctgataat
     6721 ggaggtgttc atacaaatag ccaagtgatg aattactggt tttatttgtt
//
LOCUS       DAOOQW010000124         6762 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_13800_length_6762_cov_4.691367, whole genome shotgun sequence.
ACCESSION   DAOOQW010000124 DAOOQW010000000
VERSION     DAOOQW010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 6762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6762
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13800_length_6762_cov_4.691367"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..706
                     /gene="bamD"
                     /locus_tag="PK281_07700"
     CDS             <1..706
                     /gene="bamD"
                     /locus_tag="PK281_07700"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HOY49269.1"
                     /translation="YVLAKKLYDKKDYQKAYPLLEELVSVKRGTSEAEDIYYYYCYCN
                     YYLDDLISASYHFAQFAKTYPSSKRTEEMAYMNAYCYYLGSPVPSLDQSNSYRAIEEM
                     QLFINQYPNSTRIEECNVLIDKLRAKLEIKAYDTAKLYYKMADYKAAIISMQNMLRDF
                     PMTAYKEELMFLICKSNYELAENSVDSKKYERYKSTIDAYFTFIDKFATGKYAKEAET
                     IHVKAKERFALYKPTN"
     gene            729..1049
                     /locus_tag="PK281_07705"
     CDS             729..1049
                     /locus_tag="PK281_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010179495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HOY49270.1"
                     /translation="MAVENTTVTRDLRNLEGTTGNIYESIAIIAKRANQIGLNVKEEL
                     NAKLSEFATSSDNLEEIFENREQIEISKHYERQPKPTLVAINEYQEGKVYHRGQVIES
                     TEEK"
     gene            1059..2264
                     /gene="coaBC"
                     /locus_tag="PK281_07710"
     CDS             1059..2264
                     /gene="coaBC"
                     /locus_tag="PK281_07710"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015482372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HOY49271.1"
                     /translation="MLFRKKIILGVTGSIAAYKSAALTRLLVKAGAEVRVLMTESARD
                     FITPLTLHTLSKNPVHTSFTLGPQGDWVNHVELGLWADAMIIAPASANTLAQCANGLC
                     HNLLSAVYLSARCPVFFAPAMDVDMWKHPATQQNIHQLTTNGNFIIEPGTGELASGLV
                     GEGRMSEPEDIVAALQTHFAHYRLMHGKKILMTAGPTQEPIDAVRFISNHSSGKMGYA
                     LAEELALRGARVELVSGPVTVRTQHPNIHVIPVITADEMYEETVKRFAESDVAILTAA
                     VADYQSETIAEGKMKKNAQNLNIQLKPTRDILASLGERKAAGQILAGFALETSDGRAY
                     AEKKLQSKNLDFVVLNILSKENAVFGSDLNQIEIGTAGGNWKSFDQRTKTEAARDIAD
                     HLNELMLHA"
     gene            2257..3150
                     /locus_tag="PK281_07715"
     CDS             2257..3150
                     /locus_tag="PK281_07715"
                     /inference="COORDINATES: protein motif:HMM:NF027445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4835 family protein"
                     /protein_id="HOY49272.1"
                     /translation="MRKLLILIVFILPLASSAQELNCKVSVLSQSIQLSDKRIFTTLE
                     TSVREFMNNRKWTNDQFKRDERIECNLTFNITKFTQPGDMSGTLQIQVRRPVYNANIG
                     TLLMNFIDQDIAFQYIEYQPIDFADNAYVSSLSSILGYYAYVMLAMDYDSFSLEGGTQ
                     FWQKAQQTVINSQSDGGKGWKASDIPPRNRYWLTENYLNAVYKPLREANYLYHRMGMD
                     NMYSKMDLAKSNIKEALKKIQAVNKQRPSSYNVQLFFNAKTDELINIYKQGTPAEKEE
                     ILALLAELDPTNSNKYAKINQ"
     gene            complement(3144..3644)
                     /locus_tag="PK281_07720"
     CDS             complement(3144..3644)
                     /locus_tag="PK281_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49273.1"
                     /translation="MTEAHWHLLFNHMPIMGSFFGFIILTAGIFMKQPMVRKTAYCVL
                     IFSSLLTLPAFFSGEGAEEMIEDLPTISHRLIHEHEESAEFALWLSELLGILAGVSLF
                     LEHTRHKLASVMSILTLILNLGTFASMSFVGNSGGEIRHPEIRNEKSAESEVDSTLKI
                     QEDNHH"
     gene            3838..5490
                     /locus_tag="PK281_07725"
     CDS             3838..5490
                     /locus_tag="PK281_07725"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HOY49274.1"
                     /translation="MIRVLRFCLLLLIFLVKSGFSLFAQEYPIDQKSPQSAVFKHFYY
                     LQDAHFQPEKAAEVFGNASASNQELAIQLKQIYDSRGFKPDFSSIPSDPEFTDTLINK
                     QQYAPEKGFPVIYLVRNNEKWEYSSRTMQAIPEIHHKIFPLGTGILMNLFPGTNNKTF
                     LGLKAWQYIGILILILIGFLLHLLFTILIERIVRRIIQRTNVSEEFKNAIHRMAIPIS
                     YLVLVQVMHWLFPSLLFPSDYAYYVVLGFDIVEPVFLTIGLYRLADVFSLILKRRAEQ
                     TDTTLDDQLVPLFRKAMKVFVVIFGVVAVLNNLDFNLTALIAGVSIGGLAFALAAQDT
                     IKNMFGSLMIFVDKPFQIGDLVSADGFTGTVEEVGFRSTRIRTGANSLISVPNGKLVD
                     MVVDNLGMRQFRRYKTFINLTYDTPVELIELYVKGLRQIVHLHPQTRAEENEIHVNEM
                     STSSISILFNVFFSSTSWSEELKARHEIILETIRLAEALGVRLAFPTQTLHIESFPEK
                     KSTVQTFVNPNLSGEQRFESYIQGLSERFTPKSIEQTNEHFE"
     gene            complement(5491..6072)
                     /locus_tag="PK281_07730"
     CDS             complement(5491..6072)
                     /locus_tag="PK281_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HOY49275.1"
                     /translation="MKSIVVYCGSRKGNRPAYGEAARILGKTLADKNIRLVYGGGNVG
                     LMGITADSTMQHGGEVLGIIPEFLNQLEVGHNGITELRVVENMHQRKLMMHEESDGII
                     TLPGGFGTMEEIFEMLTWGQLGMHQKPIGVLNVEGYYDDLIAQADRMVTDDFLSTRNR
                     EMLIVESDPIRLIELLVNYVPSPNPIVITPERS"
     gene            complement(6289..>6762)
                     /locus_tag="PK281_07735"
     CDS             complement(6289..>6762)
                     /locus_tag="PK281_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HOY49276.1"
                     /translation="YFDGRTCNLEGFEYAYDGQTNTIKHGTINQFILETAYTYLNAPY
                     LWGGRSPLGIDCSGFTQVVFKIAGQKLERDAYQQADQGVTLGFIDEAEAGDLAFFDNE
                     EGKIIHVGILLGAGKIIHASGRVRIDSIDHQGIFNEETGKYTHQLRILKRVLGVS"
BASE COUNT         2112 a         1381 c         1270 g         1999 t
ORIGIN      
        1 gtatgttttg gctaaaaaac tttacgataa aaaagactat cagaaggctt atccactgtt
       61 ggaagagctc gtaagtgtta aacgcggaac ctccgaagcg gaagatattt actattatta
      121 ctgttactgt aactattatc tcgacgatct catcagcgcc agctaccatt ttgctcagtt
      181 cgcaaaaacc tatccttcga gcaagcgtac cgaagaaatg gcttacatga atgcttattg
      241 ctattattta ggttctcctg ttccttcttt ggatcagtcg aatagttacc gtgctattga
      301 agaaatgcaa cttttcatta atcaatatcc aaatagtact cgtattgaag aatgtaatgt
      361 gttgattgat aagcttcggg cgaaactcga aattaaagcg tacgatacag ctaaattgta
      421 ttacaaaatg gctgattata aagcagctat aatttcgatg cagaatatgt tgcgcgattt
      481 tcccatgact gcttataagg aagaattgat gtttttaatc tgcaaatcga actacgaact
      541 agccgaaaac agtgtggatt caaaaaagta tgaacgttac aagagtacaa ttgacgccta
      601 tttcactttt attgataaat ttgcaaccgg aaaatacgcg aaagaggccg aaactatcca
      661 tgtgaaagcc aaagagcgat ttgccttata taaaccaacg aactaaaccg ttccatcgga
      721 accccactat ggctgtagaa aacaccactg taactcgcga ccttcgtaac ttagaaggaa
      781 caacaggaaa catttacgaa tcgattgcca tcatcgcaaa acgcgcaaat caaattggtt
      841 tgaacgtgaa agaagaatta aatgcaaaac tttctgagtt tgctacttct agcgataact
      901 tggaagaaat cttcgaaaac cgcgagcaaa tcgaaatttc taagcattac gagcgtcaac
      961 caaaaccaac attggttgcc atcaacgaat atcaagaagg gaaagtttat caccgcggtc
     1021 aggtgatcga atcgactgag gagaaataag cgtcctgcat gcttttccgg aaaaaaatta
     1081 tcctaggtgt tacgggtagt attgcagcat ataaaagtgc cgcacttacc cgtctcctgg
     1141 tgaaagccgg agcagaagtt cgtgtgctga tgacagaatc ggcacgcgat ttcattacac
     1201 ctttaacgct acatacttta tccaaaaatc ctgtgcatac tagttttaca ctaggaccac
     1261 aaggagattg ggtaaatcat gttgagttag gactttgggc agatgcgatg ataattgccc
     1321 ctgcaagtgc taatacgctt gctcaatgcg caaacggact ttgtcataat ttactttcag
     1381 ctgtttattt atccgctcga tgtccggtgt tttttgcacc tgccatggat gttgatatgt
     1441 ggaaacatcc tgcaacacag cagaatattc atcagctaac tacaaacggg aatttcatca
     1501 tagaacccgg aactggcgaa ttagccagtg gtttagtagg cgaggggcgg atgtcggaac
     1561 ccgaagatat tgttgcggct ctgcagactc attttgccca ttaccgactc atgcatggaa
     1621 aaaaaatcct catgactgcc ggtccaacac aagaacctat cgatgcagtg cgtttcattt
     1681 cgaatcattc atcgggaaaa atgggatatg ccttggctga agaattagcc ttacgaggag
     1741 cacgagtaga attagtctcc ggaccagtta cggttcgtac ccagcatcct aatattcatg
     1801 taatacctgt tattaccgcg gatgagatgt atgaagaaac ggtaaaacgc tttgccgaat
     1861 cggatgtagc aatactaact gcagctgttg ccgactatca atcggaaaca attgccgaag
     1921 ggaaaatgaa gaagaatgct cagaatttga acattcaatt aaaacctaca cgagatattc
     1981 tagcaagttt gggtgaacgt aaagcagctg gacagattct cgcaggcttt gctctcgaaa
     2041 caagcgacgg tagagcatat gccgagaaaa aattacaatc gaaaaatctc gatttcgtgg
     2101 tgttaaacat tctttctaaa gaaaatgctg tctttggaag tgacttaaat caaatagaaa
     2161 ttggaacggc tggtggaaat tggaaatcgt ttgatcaacg taccaaaacc gaagccgctc
     2221 gcgatattgc agatcacctt aatgaactca tgctgcatgc gtaagcttct cattcttatc
     2281 gtttttatat taccacttgc ttcaagtgct caagaattga actgtaaagt ttcggtcttg
     2341 tcgcaaagta ttcagctaag tgataaacgt attttcacca cactagaaac atctgtgcgt
     2401 gaattcatga ataaccgtaa atggacgaat gatcagttta agcgggatga acgcatcgaa
     2461 tgcaacttaa cgtttaacat taccaaattc acccaaccag gtgatatgtc tggtacgctt
     2521 caaattcaag ttcgtcgccc tgtttacaat gcaaatattg gaaccttgtt gatgaatttt
     2581 atcgatcaag acatcgcgtt ccaatacatc gaatatcaac caattgattt tgctgataat
     2641 gcatacgtat caagcttatc cagcatttta ggttattatg cctatgtaat gttagctatg
     2701 gattatgata gttttagctt agaaggcgga actcaatttt ggcaaaaggc acagcaaact
     2761 gttatcaatt ctcaatcgga tggtggtaaa ggttggaaag catctgatat tcctccacgt
     2821 aatcgttatt ggttgacaga gaattattta aatgctgttt ataaaccctt gcgtgaagca
     2881 aattatctct atcaccgaat gggaatggat aatatgtaca gcaaaatgga tttggctaaa
     2941 tcgaatatca aagaagcctt aaagaagatt caagctgtaa ataaacaaag accatcttct
     3001 tataatgtgc agttgttttt caatgctaaa accgacgaac tcatcaatat ttataagcaa
     3061 ggaactcctg ccgaaaaaga agaaattctt gccttgttag ctgagctcga tcctaccaat
     3121 tccaataagt acgcgaagat taatcagtga tggttgtctt cttgaatttt caatgttgaa
     3181 tcaacttccg attcagcgct tttttcattt ctaatttcgg ggtgacgaat ttctccaccc
     3241 gaattaccta cgaaactcat agaagcaaaa gtgcccaaat tgagtatcaa tgttaggata
     3301 ctcattacag aagctagttt gtgtcgagtg tgttccagaa ataaagaaac accagctaga
     3361 attccgagta attcagaaag ccataatgca aattcggccg attcttcgtg ttcatgaatc
     3421 aaacgatgag aaatggtagg taagtcttca atcatttcct cggcaccttc accactgaaa
     3481 aaagctggta acgtgagtaa agaagaaaaa atcagcacac aatatgcggt tttcctaacc
     3541 atcggctgtt tcatgaatat accagcggtt aaaatgataa atccgaaaaa ggaacccatg
     3601 atgggcatgt ggttgaataa taaatgccaa tgtgcctcag tcataatcaa tagaatttga
     3661 tgtaaacctc ttgatttaaa gctttcatat tacatcaccc ctgaaaagag tttcaccttg
     3721 attggttgaa aacccaccaa ttacctgtaa acaactcaac ttagttagcc agttttcaat
     3781 ttctttttag gttattttcg ccattcaaat tcattaccca atcattcgat ttaagccatg
     3841 attcgagttc ttcgattttg cttattgctc cttatttttc ttgttaaatc aggattttca
     3901 ctttttgctc aagaatatcc aatcgatcaa aaaagtccgc aaagtgctgt tttcaaacat
     3961 ttctattatt tacaagacgc tcattttcag ccagaaaaag cagccgaagt ttttggaaac
     4021 gcttcagcct cgaatcaaga actagcaatt caactcaagc agatatacga cagtcgcgga
     4081 ttcaaacccg atttttcatc tatacctagc gatccagagt ttactgatac gctaattaat
     4141 aagcagcaat acgcacccga aaaaggcttt ccagttattt acttggttcg aaataacgaa
     4201 aagtgggagt actcatctcg gaccatgcaa gcgattccgg aaattcatca taaaatattt
     4261 ccactcggaa caggcatctt gatgaatctt ttcccaggta caaacaataa aacttttctt
     4321 gggcttaaag cttggcaata catcggaatt ctcattctaa tcctcatagg atttttgcta
     4381 catctcttat ttaccatact aattgagcga attgttagac gtattattca acgtaccaat
     4441 gtatccgaag aattcaaaaa cgccattcat cgcatggcca ttccaattag ttatttggtc
     4501 ttggttcaag tcatgcattg gttattccct tccttgctat ttccctctga ctacgcctat
     4561 tatgtagtgt tagggtttga tattgttgag ccagttttcc taacgattgg tttatatcgc
     4621 ctggcggatg tgttctcgct catccttaaa cgtcgcgccg aacaaaccga taccacactc
     4681 gatgatcaat tagttccgct ttttcgtaaa gcgatgaaag tgtttgtggt cattttcggt
     4741 gtagttgctg tattgaataa cctcgatttt aacctaactg ctttaattgc aggggtttcg
     4801 attggaggtt tagcctttgc tcttgctgct caagatacta tcaagaacat gtttggctcc
     4861 ttgatgatat ttgtagacaa accattccaa attggtgatt tagtttcggc tgatggattt
     4921 acaggaacag ttgaagaagt tggatttcgt tctacacgca ttcgcacagg tgctaattca
     4981 ttgatatctg ttcctaatgg gaaattggtc gatatggtgg tggataatct tggaatgcgt
     5041 cagtttcgtc gctataaaac attcatcaac ctcacctacg atactcctgt tgagttaatt
     5101 gaattatatg taaaaggact tcgacaaatc gttcatcttc acccacaaac acgagctgaa
     5161 gaaaatgaaa ttcacgtaaa cgaaatgtcg acaagctcca ttagcattct gtttaatgta
     5221 ttcttcagct ccacttcttg gtcggaagaa ttaaaagcac gacacgaaat tattctagaa
     5281 acgattcgtc tggctgaggc tttaggtgtt cgcttggcat tcccaacaca aacgctgcat
     5341 atcgaaagtt tccccgaaaa gaagagtacg gttcaaacat tcgtaaatcc gaatctttca
     5401 ggagaacagc gtttcgaaag ttatatacag ggattgtctg aacgattcac tcccaagtcg
     5461 attgaacaaa ccaacgaaca tttcgagtaa ttagctacgc tcaggagtaa taacaattgg
     5521 attcggactc ggaacgtaat taaccagtaa ctctattaat cgaattggat cagattcaac
     5581 aatcagcatt tctcggtttc gtgtactgag aaaatcatct gttaccatac ggtcggcttg
     5641 cgcaattaaa tcatcgtaat atccttctac attcagcact ccaatgggtt tttgatgcat
     5701 gcctaattgt ccccatgtga gcatttcaaa aatctcttcc atcgttccaa atccgccagg
     5761 aagagtaata attccatccg attcttcgtg catcataagt tttcgttgat gcatgttttc
     5821 taccacacgt aattcggtaa ttccgttatg tccaacttcc aattgattca aaaattctgg
     5881 tataatcccc aaaacttcac caccatgttg catagttgaa tcggctgtaa tgcccatcaa
     5941 acccacattt cctccaccat atactaaacg aatgtttttg tcggctaagg ttttacctaa
     6001 tatacgagca gcttccccat acgcaggtcg atttccttta cgtgatccac aatacacaac
     6061 gattgatttc ataccaaaaa attaagcagc aaacatacaa ggatggctgc tgcttttagt
     6121 gtgtgcgtga aaagtttacc gattggtaac agaacattag caaagactta ttgctaatct
     6181 taaaatctaa aagtcctgaa ttaaaagtag gaaattctag ccaaaacgga aattcaaaaa
     6241 attgaaattc agagaatgaa gcaattttcc taatgccgaa atattcgatt aggaaactcc
     6301 taaaacgcgt ttaagaatgc gtaattgatg cgtgtatttt cctgtttctt cgttgaaaat
     6361 accctggtgg tcgatactat cgattcgaac acgtcccgat gcatgaataa tttttccagc
     6421 acctaataat attccaacat ggatgatttt accttcttca ttatcgaaga atgctaaatc
     6481 acccgcttcg gcttcatcaa taaatcctaa agttactcct tgatccgctt gctgataggc
     6541 atctcgctca agcttttgtc cggcaatctt aaatactact tgtgtaaatc cggaacaatc
     6601 aattcctaat ggagatcgtc cgccccagag ataaggagca ttcaaatacg tgtaagctgt
     6661 ttctaaaata aattggttga ttgtaccgtg tttgatggtg ttggtttgac catcatacgc
     6721 atattcaaaa ccttccaaat tacaagttct accatcgaaa ta
//
LOCUS       DAOOQW010000125         6742 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_13843_length_6742_cov_4.197697, whole genome shotgun sequence.
ACCESSION   DAOOQW010000125 DAOOQW010000000
VERSION     DAOOQW010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 6742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6742
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13843_length_6742_cov_4.197697"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="PK281_07740"
     CDS             <1..440
                     /locus_tag="PK281_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="diheme cytochrome c-553"
                     /protein_id="HOY49277.1"
                     /translation="IMGCNDCHSPKRMGEKGPEIIPELLLSGYPADRPIVKFDSKLIK
                     EGFAMLYPDLTGAAGPWGVSFAANLTPDESGIGNWTEEQFKKALKEGKYKGMDGGRML
                     LPPMPWVNYINLADEDLHAMFMYLKSLKPVKNIVPQPITPDKM"
     gene            538..1092
                     /locus_tag="PK281_07745"
     CDS             538..1092
                     /locus_tag="PK281_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014083796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOY49278.1"
                     /translation="MKHSSLLLFGEESERLSYRKVDDSYFPEWLKFCSDEDSLRYIGI
                     PEGYSPQEKCQIWFDRVYNRYENNLGGMNALVTNQNNLFIGQCGLLVQTVDEIQELEI
                     GYSLMPNHRNQGYAIEAAKKCRDYAFENNLADSLISTIHVDNVSSAKVAVNNGMKLEK
                     KTLFKGLPVNIYRISKSDWNRLSK"
     gene            1316..2068
                     /locus_tag="PK281_07750"
     CDS             1316..2068
                     /locus_tag="PK281_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015023407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HOY49279.1"
                     /translation="MTEAYVQHTLAEGIATITFFHPDQNSLPGTILAQLADTITQVGQ
                     LDEVKVIILKSGGDRTFCAGASFKELITINDAETGKIFFSGFANVINAMRKCPKFILG
                     RVQGKTVGGGVGLAASTDYCFATKYASVKLSELNVGIGPFVVSPAIERKMGISAMSQI
                     AIDANTFYDATWALQKGLYAQVFDSIEEMDAAIEAFAKHLCTYNPDAMKEMKQIFWRG
                     TDDWDKLLADRAAISGRLVLSDFTKTTLQKFK"
     gene            2078..2671
                     /locus_tag="PK281_07755"
     CDS             2078..2671
                     /locus_tag="PK281_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013870249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase hexapeptide repeat family protein"
                     /protein_id="HOY49280.1"
                     /translation="MVYEFNGFIPVVHESSFIHPLAAVIGNVIIGKDVYVGPGAAIRG
                     DWGGIIIEDGCNVQENCTIHMFPGKDITLKKGAHIGHGAIIHGANIGANCLVGMNAVI
                     MDDVDLGDECIVGALSFVKAGMKIPNRKMIVGNPAQIIKDISDEMIDWKTRGTALYQQ
                     LPKECFDTLKEVEPLREIPANRPTQQAVYPTLGEFKK"
     gene            2679..3938
                     /locus_tag="PK281_07760"
     CDS             2679..3938
                     /locus_tag="PK281_07760"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013870255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HOY49281.1"
                     /translation="MPKIEFKLPRMGESITEATILKWMKNVGDFIDVNETLLEVGTDK
                     VDSDVSVPFAGIIQEIYFKNGDVVPVGAVLALIEADDSAIVQETEGSSSPELINQTEK
                     PRAQAGNIQPLGFLSPLIRNIVIAEGITLEELQHVTGSGHHGRIQKSDLFSYLKNRLE
                     PSKRHSSPTTISTFPKPSISFIEGHDQVIEMDRMRKMIADHMVYSKHTSPHVTAYIEI
                     DVTNLSNWRNRVKDDFQQKYGQKLTFTPLFVDAVTRAITDFPMINASIDGNKIIVHKD
                     IHIGMATALPSGNLIVPVVRDSAGKTLSQLAENVNALADNARNNQLRPDDVQGSTFTI
                     SNVGTFGSLMGTPIINQPEVAILAFGVIKRRAEVILTQGKEEVAIRSMMFLSLSFDHR
                     AVDGYLGGSFLRRVGDYLEQFDINSAI"
     gene            3953..5014
                     /locus_tag="PK281_07765"
     CDS             3953..5014
                     /locus_tag="PK281_07765"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HOY49282.1"
                     /translation="MSYEITITRASHSKIGETDIDHIPMGICFTDHMFVCDYHEGTWQ
                     NPRIEPLAPIPTHPSAMALHYGQAIFEGMKVTRGKNGTPILFRPYENAKRLNFSADRM
                     AMPHFPETLFVEALKMLVDIERQWIPTTEGSALYLRPFMYADESYTGMRAATSYKFII
                     FATPAGPYFNRRIRLYAETKFIRAANGGTGEAKAAGNYGAAIRPTEIAKANGYDQVLW
                     LDATHFENIMEVGTMNIFFKINGKFITPILDGSVLAGITRSSVIDLLRYKGYEVYERT
                     ISMKEILEASENNSLEEAFGTGTAVGIAMIEEIGYKNLFIHLPAENPVATMVNETINR
                     IKVQEEADPFGWTIPVLTN"
     gene            complement(5011..6228)
                     /locus_tag="PK281_07770"
     CDS             complement(5011..6228)
                     /locus_tag="PK281_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006988693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOY49283.1"
                     /translation="MNKEIMVELDSLFERNARKLAQTELKHIRYIMDEIDWKQRLIGL
                     KGARGVGKTTLLLQRLKLLKLPANKALYVSCDNIWFSQHSIVELAETFVREGGLFLFL
                     DEVHKYPNWAQELKNIYDDFPELHTIFTGSSLLEILNARADLSRRAIVYDMQGLSFRE
                     FLIFETEHSFPMITINDLLINHEERAIEICKKLRPLEHFRDYLQFGYYPFYRESINLY
                     PSRVEEIISMVLYIELPLLRKLDVPYIPKIKQLLHIIAESAPFVPNVSKLSERIGINR
                     NTFISYLFYLNEAGLTRNLYKDIKGVSRLQKPDKIYLDNPNLHYVLSSQKPNIGHVRE
                     CFFLNQLQYNHSVEYINDGDFCADGKFTFELGGKLKSAKQLKGASDAWIVSDSIEIGT
                     GIRIPLWMFGFLY"
BASE COUNT         2103 a         1236 c         1351 g         2052 t
ORIGIN      
        1 gtataatggg ctgtaacgat tgtcattctc caaaacgaat gggggagaaa ggaccggaaa
       61 ttatacctga attattactt tctggttatc ctgctgatcg tccaattgtt aagtttgata
      121 gtaaattaat aaaagaagga tttgccatgt tgtatcccga tttaactggc gctgcaggtc
      181 cttggggtgt ttcttttgct gccaatttaa ctcccgacga atcgggtatt ggtaattgga
      241 cagaggagca atttaaaaaa gcattgaaag aaggtaaata caagggaatg gatggcggaa
      301 gaatgctttt acctcctatg ccatgggtga attatattaa tcttgcggat gaagatttac
      361 atgccatgtt tatgtatctc aaaagcctca agccggttaa aaacatagtt ccacagccaa
      421 tcacacctga taagatgtag atttttcgag gctaatttca tttaattctt ttgtttaaat
      481 tcaattactt aattgtttag gatttaattc taaatacaat ttgattttca ccattttatg
      541 aaacactcta gtctcttgct ttttggtgag gaatctgaac gattatctta tcgtaaagta
      601 gatgattcct attttcctga atggttgaaa ttttgttccg atgaagattc attgagatat
      661 attggaattc cagaaggata ttcacctcaa gagaaatgtc aaatctggtt tgatcgtgtt
      721 tataatcgct atgaaaataa tcttggagga atgaacgcac ttgttacaaa tcaaaacaat
      781 ctgtttattg ggcaatgtgg acttctagtt caaacggttg atgaaattca agaattagaa
      841 attggatatt ctttaatgcc taatcatcga aatcaaggtt atgccattga agctgcaaaa
      901 aaatgtcgtg actatgcatt tgaaaataat ttagcagact ccttaatatc taccattcat
      961 gtggataatg taagttcagc taaagttgct gtgaataatg gaatgaaact cgaaaagaaa
     1021 acattattca aaggattacc agtcaatatt tatagaattt caaaatcaga ttggaatcgt
     1081 ttatccaaat gagcacttat tttttcaact gaaaagcaat cagttcgtta tccatattta
     1141 gtttagagat taacatacaa cgaaaattgt ttgccaaata aagttttgtg aaagctgcga
     1201 atgctcttgt atattgcttg aattcgcaat gtgcccggaa taatatttgg caaaattagc
     1261 gtttgctaaa acatctaatt tgttattttt gtaacgtacc caatgcgtaa cttctatgac
     1321 cgaagcctat gttcagcaca cgcttgctga aggaattgca accatcacct tttttcatcc
     1381 cgatcaaaac tcgcttcctg gaactattct agcacaactg gctgatacga ttacgcaagt
     1441 cggacaacta gatgaagtaa aagtgattat ccttaaaagt ggaggagatc gtacgttttg
     1501 tgctggtgcg agttttaagg aactcatcac gatcaatgat gctgaaactg gaaaaatctt
     1561 cttttcagga tttgcaaacg tgataaacgc catgcgtaaa tgcccgaaat ttattttagg
     1621 tcgcgtccaa ggcaaaactg ttggtggtgg agttggttta gctgcttcaa ctgattattg
     1681 ctttgctaca aaatatgcat ccgtaaaact aagcgagctc aatgtgggaa tcggtccatt
     1741 tgtggttagt cctgcaattg agcgaaaaat gggtatttct gccatgtccc agatcgccat
     1801 tgatgccaac acgttctacg atgcaacttg ggcgcttcaa aaaggtttat atgctcaagt
     1861 atttgatagc atcgaagaaa tggatgctgc catcgaagca tttgctaaac acttgtgcac
     1921 ctataatcca gatgcaatga aggaaatgaa gcaaatcttc tggcgtggaa cagatgattg
     1981 ggataaactt ctagccgatc gtgctgccat tagtggacgt ctcgtattgt cggacttcac
     2041 caaaacgaca cttcagaagt tcaaataatt tccttctatg gtatacgagt ttaacggttt
     2101 cattcccgtt gtgcatgaaa gtagttttat ccatccttta gcagctgtta ttggaaatgt
     2161 gataattgga aaagatgtat atgtaggtcc tggtgctgct attcgtggtg attggggggg
     2221 aattattatc gaggacggtt gtaatgttca ggaaaactgc accattcaca tgtttccagg
     2281 gaaagatatc accttgaaaa aaggagctca tattggacat ggtgccatca ttcacggagc
     2341 taatattggt gccaattgcc tggtgggtat gaatgctgta ataatggatg atgtggattt
     2401 aggtgatgaa tgtatagttg gtgcattatc atttgtgaaa gcaggtatga aaatccctaa
     2461 ccgtaaaatg atagtcggaa atcctgcgca aatcatcaaa gatattagtg atgaaatgat
     2521 tgattggaaa acgcgtggaa cagctttata tcagcagtta cccaaggaat gtttcgacac
     2581 tttaaaagag gttgaacctt tacgtgaaat cccggcaaac cgaccgactc aacaagctgt
     2641 gtatcctaca ttaggtgagt tcaagaaata ataaagcaat gcctaaaatc gaatttaaat
     2701 tgccccgaat gggcgaaagc atcaccgaag caaccattct aaaatggatg aaaaatgtcg
     2761 gtgatttcat cgatgtgaat gaaacattgc tcgaagtagg aacagataaa gtcgatagtg
     2821 atgttagtgt tccctttgct ggtataattc aagaaatata cttcaagaat ggtgatgtcg
     2881 ttcctgttgg agctgttcta gcattaattg aagctgacga ttctgctata gttcaagaaa
     2941 cagaaggatc ctcttcaccc gaattaataa atcaaactga aaaaccaaga gctcaagcag
     3001 gtaatattca acccttaggt tttctttctc ctcttatacg aaatattgta atagcggagg
     3061 gaattacact agaagaattg caacatgtta ctggctctgg tcatcatggt agaattcaaa
     3121 aaagtgatct tttttcctac ctgaaaaatc gcctcgaacc atccaaacgt cattcatctc
     3181 caacaaccat ttcaaccttt cctaaacctt caatttcatt tatagaagga cacgatcagg
     3241 tgattgaaat ggatcgcatg cgtaagatga ttgctgatca tatggtgtat tcaaaacata
     3301 cttcaccaca cgtgacagct tacatcgaaa ttgatgtaac taatctaagt aattggcgaa
     3361 atcgggtgaa ggatgatttt caacagaaat acggacaaaa actcaccttt actccacttt
     3421 tcgttgatgc ggtaacacgc gccattacag actttcctat gattaatgct tccattgatg
     3481 gaaacaaaat cattgtccat aaagacattc atattggtat ggccactgct ttaccaagtg
     3541 gaaatttaat cgttccagtt gttcgagatt cggctggaaa aacgttgtct caacttgcgg
     3601 agaatgtgaa tgcattagcc gataatgctc ggaataatca attacgccct gacgatgtgc
     3661 aaggtagcac ctttaccatt tccaatgtgg ggacttttgg aagtttaatg ggaacgccaa
     3721 ttatcaatca acccgaagta gccattttag catttggggt gataaaaaga cgtgccgaag
     3781 tcatcctaac acaaggcaaa gaagaagtcg ctatccgaag catgatgttt ttgtcgcttt
     3841 cctttgatca ccgtgctgtt gacggatatt tgggaggttc atttttacgt cgggtagggg
     3901 attacttaga acaatttgac atcaactcag ctatctagct ttcacaacca tcatgagtta
     3961 cgaaattacc atcacacgcg catcacattc gaaaatcgga gaaaccgaca tcgatcatat
     4021 tcctatgggg atttgtttta ccgatcacat gtttgtgtgt gattatcacg aaggaacttg
     4081 gcagaatcca cgcattgaac cattggcgcc cattccaaca catccatctg caatggcctt
     4141 gcattacggt caggctattt tcgagggaat gaaagttact cgcggaaaga acggaacgcc
     4201 tattttattt cgtccatatg aaaatgcaaa acgactgaat ttcagtgctg atcgtatggc
     4261 tatgccgcat ttccctgaaa ccttgtttgt agaggctttg aaaatgctcg ttgatattga
     4321 gcgtcaatgg attccgacaa ccgaaggaag tgctttgtat ctgaggcctt ttatgtatgc
     4381 ggatgaatca tacacgggaa tgcgagcggc aactagttac aagtttatca tttttgctac
     4441 acctgctggt ccgtatttca atcgtagaat tcgattatat gcggaaacaa agtttatccg
     4501 tgctgcaaat ggtggaacgg gcgaagcaaa ggctgccgga aattatggtg cagcaattcg
     4561 tccgacagaa atagctaaag caaacggata tgatcaagtg ttatggcttg atgctactca
     4621 ctttgagaat atcatggaag ttggtacgat gaatattttc ttcaagataa atggaaagtt
     4681 tatcacaccc attttagacg gttcagtatt agcgggaata acacgaagta gtgtaatcga
     4741 tttattacgt tacaaaggtt atgaggttta tgagcgaacc atttcgatga aagaaatatt
     4801 ggaagccagt gaaaataatt ccctagaaga agcatttgga acgggcactg ctgtaggaat
     4861 tgcgatgatt gaggaaattg gatataaaaa tctattcatt catctacctg cagaaaaccc
     4921 tgttgcaacc atggtaaacg aaacgattaa tcggattaaa gtacaggaag aagcagatcc
     4981 atttggttgg acaatcccag ttttaactaa ttaatagagg aatccaaaca tccaaagcgg
     5041 tatacgaata ccagttccaa tttcaatact gtcagaaaca atccatgcat cagacgctcc
     5101 tttgagttgt ttggctgatt ttagctttcc tcctagttca aaggtgaatt ttccatcagc
     5161 acaaaaatcg ccgtcgttga tgtattcaac gctgtgattg tattggagtt gattgagaaa
     5221 aaagcactct ctaacatgtc caatattcgg cttctgacta cttaatacat agtgaagatt
     5281 tggattgtct aagtatattt tatccggttt ttgtaaacgc gaaacgcctt taatgtcctt
     5341 ataaagatta cgtgtgagcc ctgcttcatt caaataaaat aaataagaaa tgaaggtgtt
     5401 tcgattgatt ccaatacgtt cacttagttt cgaaacattt ggaacaaaag gcgccgattc
     5461 agcaatgata tgaagtaatt gtttgatttt aggaatatat ggaacatcca atttccggag
     5521 caatggcagt tcgatgtata aaaccatgct gatgatttcc tctacgcggg atgggtataa
     5581 attgattgat tcgcgataaa atggataata gccaaattgt aaataatctc tgaaatgctc
     5641 caaagggcgc agttttttgc agatttctat ggctcgttct tcatgattaa tcaataagtc
     5701 attgattgtg atcatcggaa aggaatgttc tgtttcaaag atcagaaact cacgaaatga
     5761 taaaccttgc atgtcatata caattgcacg ccgactcaaa tctgctcgcg catttaggat
     5821 ttctaaaagc gaagaaccag taaatatggt atgtaattca ggaaaatcat cataaatgtt
     5881 tttaagctcc tgtgcccaat tagggtattt gtgaacttca tccaaaaaca aaaacaggcc
     5941 gccctctcta acgaacgttt cagccaactc tacaatcgaa tgttgactaa accaaatgtt
     6001 atcgcagctt acatataacg ctttattcgc ggggagtttg aggagcttta gccgttgaag
     6061 caataaggtc gttttgccca cacctcgcgc acctttaagt ccaattaaac gttgcttcca
     6121 atcaatttca tccatgatat agcggatgtg tttgagttct gtttgcgcta gttttcgtgc
     6181 attacgttca aataaggaat cgagctctac cattatttct ttattcatca acaaagatag
     6241 caatatcatt ctgattatgc ttaatacaat aagcaaattg aattttttgt tgttaaatat
     6301 cagtaaaaca gatagttgag tgtgctattt ttgattgcat ccctcgtaag aactctctta
     6361 acccgccttt gattgctttt caatttccat cttttattca aacaactatc aaaatacagc
     6421 ttcagaacta attgtaattc aaatatttat atattaaaat agcattttgc ttactatggt
     6481 aagcaatatt tgcaaccttt tgcaaataac tcatttcctt cttgtcaggc agcctgatat
     6541 tcccttctat caaaacgact tttgctatct tcgcgccctc tatgcaaggc attccaagga
     6601 acatactcga aaaagcattt agtctgatgg ctaccgctaa agccatgact gaattgtatg
     6661 aagccaattt caaagtggtg tcgaaatatg ttcatgctac atcacgcgga catgaagcca
     6721 ttcagctagc cacggccttg ca
//
LOCUS       DAOOQW010000126         6505 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_14498_length_6505_cov_4.654264, whole genome shotgun sequence.
ACCESSION   DAOOQW010000126 DAOOQW010000000
VERSION     DAOOQW010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 6505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6505
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14498_length_6505_cov_4.654264"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..755)
                     /locus_tag="PK281_07775"
     CDS             complement(<1..755)
                     /locus_tag="PK281_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HOY49284.1"
                     /translation="MGKIIGIDLGTTNSCVAVMEGNEPVVIQNSEGKRTTPSIVAFLD
                     NGERKVGDPAKRQAITNPTKTISSIKRFMGNGFSEVQTEAGRVPYKVVKGDNNTPRVQ
                     IDDRTYTPQEISAMVLQKMKQTAQDYLGTDVTEAVITVPAYFNDAQRQATKEAGEIAG
                     LKVMRIINEPTAAALAYGLDKKNADMKIVVFDCGGGTHDVSVLELGDGVFEVKSTDGD
                     THLGGDDFDQKIIDWLAEEFASTDGEGLDLQLT"
     gene            complement(907..1923)
                     /locus_tag="PK281_07780"
     CDS             complement(907..1923)
                     /locus_tag="PK281_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49285.1"
                     /translation="MRKLLVFITGLVTLFTSSCSNDLDINAPWKDITVVFGLLNKDQT
                     THYIRVSKAFLGEGDALEFASQFDSLYYNPNLLEVKVEEILNGNVTRTFICEPDSSIA
                     KDPGIFSAPSQILYKFETSAAQKINPSATFRLTVTNISSGNSVTSETVLVKNLVMSSP
                     NQLIDETNLYPQNATFIKWKTAENGKMYELFLRFKYREYSELNPTEIVSKQIELSLGR
                     ITTDNTNPNNEMTMNVENSTIYQAISAAISPSTTDNPMVRICDSLQFQINVAAEDLTT
                     YLNVNKPSNTIAQERPQFTNINNGLGLFSSRVALIRTIYLNDFTVDSLRGNPLTEDLN
                     WQPR"
     gene            1993..2808
                     /gene="panB"
                     /locus_tag="PK281_07785"
     CDS             1993..2808
                     /gene="panB"
                     /locus_tag="PK281_07785"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HOY49286.1"
                     /translation="MSVQKEIKRVTTKTLQEMKNKGEKISMLTAYDFSMAGMVDEAGI
                     DVILVGDSASNVMAGHETTLPITLDQMIYHASSVIRGTSRALVVVDLPFGSYQGNSKE
                     ALTSAIRVMKETGAHAIKLEGGEEVVESVKRILSAGVPVMGHLGLTPQSIYKFGTYVV
                     RAKEEEEAQKLIQDAHLLQEAGCFAIVIEKVPAKLAERVAKEVAIPIIGIGAGGGVDG
                     QVLVLHDMLGINTEFKPRFLRTYLNLHEQIVSAIGNYVKDVKSSDFPNGSESY"
     gene            2789..3535
                     /locus_tag="PK281_07790"
     CDS             2789..3535
                     /locus_tag="PK281_07790"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOY49287.1"
                     /translation="MDPNPINSPGISDISGSLGSPGLNSGGTVLFEDNHLIAVNKQAG
                     EIVQGDKTGDVPLSEKVAEYLRVKYQKPGDAFIGVIHRIDRPVSGVVLLARTSKALSR
                     MNEQFRLRTIKKTYWALVEGKVEQEEGTLIHYLSKNEEKNTSRALKEPRDGYLRCELS
                     FKVIRVLDRYTLLEVEPLTGRHHQIRVQLSAMGWSIKGDVKYGARRGNPDRSICLHAR
                     MLRFEHPVKKEPIEITAALPGTEAWIGLKD"
     gene            complement(3690..3911)
                     /locus_tag="PK281_07795"
     CDS             complement(3690..3911)
                     /locus_tag="PK281_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003441576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HOY49288.1"
                     /translation="MYWTLELASYLEDAPWPATKDELIDFGIRSGAPLEVIENLQEIE
                     DEGEVYESIEEIWPDYPTKDDFFFNEDEY"
     gene            complement(4025..4573)
                     /locus_tag="PK281_07800"
     CDS             complement(4025..4573)
                     /locus_tag="PK281_07800"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013187563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOY49289.1"
                     /translation="MKIYTRTGDAGQTSLLGGTRVPKHHLRIEAYGTLDELNSHIGFV
                     RDQGLSADATAQLIEIQDRIFTLGSHLASDPEKSKAKIPDLKPEDIEFLELAIDAMNE
                     QLPEMRHFILPGGHPAVSACHIARCVCRRAERLIVHLSEISPVEASVIQYINRLSDYL
                     FVLSRKISSDFNANETPWKPRM"
     gene            complement(4604..5323)
                     /locus_tag="PK281_07805"
     CDS             complement(4604..5323)
                     /locus_tag="PK281_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008253937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOY49290.1"
                     /translation="MNSSNNVIQLRNIIRNFRLGSETIQVLRSINLDISRGEYVALMG
                     PSGSGKSTLMNILGCLDTPTSGEYELNGQLVSKLSDNELADIRNKEIGFIFQTFNLLP
                     RSTALDNVILPLIYAGESKTDRDTKARKALDQVGLSDRIMHKPNELSGGQRQRVAIAR
                     ALVNTPSILLADEPTGNLDTKTSIEIMGLLHDIHAQGNTIIIVTHEEDIAQHAHRIIR
                     LRDGVIENDYLNENIRVYQKS"
     gene            complement(5374..6147)
                     /locus_tag="PK281_07810"
     CDS             complement(5374..6147)
                     /locus_tag="PK281_07810"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOY49291.1"
                     /translation="MLARIGLYISYWLRAKGPNRVHSPFVFSLYTDVILPDNQYYAFE
                     AIEKIRTKLLENNTLISDSDPGAGSKSNPHQKTVRSIAQSSLLSPKPGRLLFRLTNYL
                     QPKTILELGTSLGISALYLAKPTSAQVHTIEGRPSLVKLAQQNAKHLNAQNITVHSGR
                     FEEVLPSLLPQLKQVDMLYLDGNHTLEASLRYFEQIRPYMHSDTVIIMDDIRWSLDMM
                     KAWLVLSEQTDIHVSVDLQKLGLLFFRPSQVKEHFILRV"
BASE COUNT         1800 a         1161 c         1612 g         1932 t
ORIGIN      
        1 ttggttagct gaagatctaa accttctcca tcggttgaag caaattcttc agctaaccaa
       61 tcgatgattt tttgatcaaa gtcatcacca cctaagtggg tatcaccatc ggttgatttt
      121 acctcaaata caccatcacc taattccaaa acagaaacgt cgtgtgtacc accaccacag
      181 tcgaatacca caattttcat atcggcattc ttcttatcta aaccataagc taatgcagct
      241 gcagttggtt cgttgatgat acgcatcact ttcaatccag caatttcgcc tgcttctttg
      301 gtagcctgac gttgtgcatc gttaaagtat gctggtacgg taataaccgc ttcggtaaca
      361 tctgttccca agtaatcttg agctgtttgc ttcattttct gaagcaccat agcagaaatc
      421 tcttgtggag tatatgtacg gtcgtcgatt tgaactcgtg gtgtgttatt gtcgccttta
      481 actactttat aaggaactct tcccgcttcc gtttgaactt cggaaaatcc gttgcccata
      541 aagcgtttaa tagaagagat tgtttttgtt ggattagtga tggcctgacg tttagccgga
      601 tcaccaacct tacgctcacc attatcgagg aatgctacta tggaaggtgt tgtgcgcttg
      661 ccttcgctat tttgaataac taccggctca ttgccttcca tcacggctac gcaggagtta
      721 gttgttccca agtcaatgcc tatgattttt cccattgttt tattttttat tcggttagtt
      781 tctagtgtgc agcccggttt tcaatgtctg tgccacctaa aaaacaaacc ctgtgcttgt
      841 cagttttgac agtacagggt ttgttttgct ccaattctgg cagcaaagcg gtgacaattc
      901 ggcagattat ctaggctgcc agtttaagtc ttctgtcaat ggatttccac ggagtgagtc
      961 aaccgtaaaa tcgttgaggt aaatggtacg aattaaagct acacgagaag aaaataaccc
     1021 caatccattg ttgatgtttg tgaattgtgg tctttcttgt gcaattgtat tggatggctt
     1081 atttacattc aaataagtag ttaaatcctc ggcagctaca ttgatttgaa actgcaaact
     1141 atcgcaaatg cgcaccatgg gattgtcggt agtagaagga ctgatagctg cagaaatagc
     1201 ttgatagatt gtcgaatttt cgacattcat ggtcatttcg ttattcggat tggtgttatc
     1261 ggtagtgatg cgaccaaggc taagttcgat ttgtttggaa acgatttcgg ttggatttaa
     1321 ttcgctgtat tctcggtatt tgaagcgaag aaacaactca tacattttac cattttcggc
     1381 tgttttccat ttgatgaaag tagcattttg aggatataag tttgtttcat caataagctg
     1441 attgggtgaa gacatcacga gatttttcac caaaacagtt tcggaggtaa ccgagtttcc
     1501 agaggaaata ttggtaacag ttagtcggaa tgtagcacta ggattgattt tttgagcagc
     1561 acttgtttca aacttgtaga gaatttgtga gggtgcagaa aatattcccg gatcttttgc
     1621 aatgcttgaa tcgggctcac aaataaatgt acgcgtaacg tttccattta aaatctcttc
     1681 cacctttact tccagaagat ttggattgta ataaagcgag tcgaattggg atgcaaattc
     1741 caatgcatct ccttcgccta gaaaggcttt gcttactcga atgtaatggg ttgtttgatc
     1801 tttattcaat aagccaaaca cgacagtaat gtctttccaa ggtgcattga tgtcaagatc
     1861 gttggagcag gaactcgtga aaagagtaac taaaccagta ataaaaacta atagtttacg
     1921 catagcggca aaagtaggaa tttcatcaca gtgagtaaat gactaatatt gccctttatc
     1981 ggataaaaaa ctatgtcggt tcaaaaggaa atcaaacgcg tcaccaccaa gacgttgcaa
     2041 gaaatgaaga acaaaggtga gaagatttcg atgcttactg catacgattt ctcgatggct
     2101 ggtatggtgg atgaggctgg aatagatgtg attttggttg gtgattcggc ttcgaatgtg
     2161 atggctggtc acgaaaccac tttacctatt acgctcgatc agatgattta tcatgcttca
     2221 tcggtgattc gcggaacttc gcgtgcattg gtggtagtgg atttgccttt tggttcctat
     2281 caaggaaatt cgaaagaagc gcttacgtca gctattcgcg tgatgaaaga aacaggtgcg
     2341 catgcgatta agttggaagg tggagaagaa gtggtagaat cggttaagcg gattttgagt
     2401 gcaggtgttc cggtgatggg acatttgggt ttgactcctc aatcgatcta taaatttgga
     2461 acctacgtgg ttcgtgctaa agaggaagaa gaagcgcaga aacttataca ggatgcgcat
     2521 ttgttgcagg aagcgggatg ttttgccatt gtgatagaaa aagtaccagc aaaattggcc
     2581 gagcgtgtag ctaaggaagt agcaattcct attattggta ttggtgctgg tggtggagtt
     2641 gacggacaag tattggtatt acacgatatg ttgggaataa ataccgagtt caaacctcgt
     2701 tttttacgta cctatctcaa tttacatgag caaattgtta gtgcgattgg gaattatgta
     2761 aaagatgtga aatcgagcga ttttccgaat ggatccgaat cctattaata gccccggaat
     2821 ctctgatatt tccggcagcc tcggcagccc cggacttaat tccgggggta ctgtattgtt
     2881 tgaggataac catctgattg cggtaaataa gcaagctgga gaaattgtcc aaggcgataa
     2941 aacgggagat gttcccttga gtgaaaaagt tgccgaatat ttacgagtaa aatatcaaaa
     3001 gccgggtgat gcctttattg gtgtgattca tcggattgat cgtccggtta gcggtgtggt
     3061 tttgctggcg cggacgagca aagcattatc gcgcatgaat gagcagttta ggttacgaac
     3121 gattaagaaa acctattggg cattggtaga agggaaagta gagcaggaag aaggaacgct
     3181 gattcattat ttaagtaaga acgaggagaa gaatacttca cgtgcattga aagagcctcg
     3241 agatgggtat ttgcgttgcg aattgagttt taaagtgatt cgcgtgttgg atagatatac
     3301 cttattggag gtagagccat taacaggaag gcatcatcag attcgggtgc agttatcggc
     3361 gatgggatgg tctattaagg gtgatgtgaa atatggagca cgacgtggaa atcccgaccg
     3421 gagtatttgt ttgcatgcgc ggatgttacg atttgagcac ccagttaaaa aggaaccgat
     3481 tgaaattacg gcagctttac ccggcacaga agcttggata ggattgaaag attgatgccc
     3541 taacgttagc accagggatg taagccttcg ctaaagctac ggcttacgct gtactatcct
     3601 ttaggcaagc agcgaacagc ccgcccgggt gccataaaaa aagcgctact ctttgcagaa
     3661 cagcgcttgt atcttgaaga ataagaaaat cagtattcgt cttcgttgaa gaagaagtcg
     3721 tcttttgtcg gataatccgg ccagatttct tcaatacttt catacacttc gccttcgtcc
     3781 tcaatctctt gcaggttctc gataacctcc agaggggctc cagaacgaat gccgaaatcg
     3841 attagctcgt ccttagtagc aggccaaggc gcgtcttcaa ggtaagaggc aagttctagt
     3901 gtccagtaca tttccttggt attagggtga ttaattaatg ggtgaactga agttttcggc
     3961 ttagtgcgtc aactttccgc aaaaatagaa ataagaagat aggtttgttg cgcttggtga
     4021 tattttacat ccttggtttc caaggagttt cgtttgcgtt gaaatcggaa ctgattttac
     4081 gggagagaac gaagaggtaa tcggacaatc ggttgatgta ttggataacg cttgcttcga
     4141 ccggactgat ttcggaaaga tgtacaatta gtcgttcggc tctacggcaa acacagcgtg
     4201 caatatgaca tgccgaaaca gctggatgtc cgcctggtag gatgaagtgg cgcatttcgg
     4261 gaagttgctc gttcatggca tcgatggcca gttcgagaaa ttcgatgtct tcgggtttta
     4321 ggtctggaat tttggctttg cttttttcgg gatcggaagc aaggtgcgat cctaacgtga
     4381 agatacgatc ttgaatttcg atgagttgag cggtagcatc ggcacttaaa ccttgatcgc
     4441 gaacgaaacc aatatgtgaa ttaagttcgt cgagcgttcc gtaagcttcg atacgcaagt
     4501 gatgtttggg aacacgtgtt ccgccgagca gcgaagtttg tccggcatca ccggttcgag
     4561 tatatatttt cataatcgcc aaacgagata agcgggtgaa agtttagctt ttttggtaaa
     4621 cgcggatgtt ttcgtttagg tagtcatttt cgataactcc atcacgaagg cgaataatcc
     4681 ggtgagcgtg ctgtgcaatg tcttcctcgt gtgtaacgat aataatggtg tttccttgtg
     4741 catgaatatc gtgcagcaaa cccatgattt cgattgaagt tttggtgtcg aggttacctg
     4801 taggttcgtc agccaagagg atagatggag tatttaccaa tgctcgagca atagctacac
     4861 gctgacgttg accacccgaa agctcgttag gtttgtgcat gatacggtcg ctcagtccaa
     4921 cttgatcgag tgctttacgc gctttggtgt ctcgatcggt tttagattcg ccagcataaa
     4981 tgagtggcag aattacattg tcgagcgcgg tggaacgagg aagtaggttg aatgtttgga
     5041 agatgaaccc aatttccttg tttcggatgt cggcaagttc gttgtcgctt aatttagaca
     5101 cgagttgtcc attgagttcg tactcgcccg aagtaggagt gtcgagacag ccgaggatgt
     5161 tcatcagagt cgattttcct gaaccggaag ggcccatgag tgccacatat tcgcctcgag
     5221 agatgtcgag attaatggag cggagcactt gaatggtttc ggaaccaagc cggaaattcc
     5281 ggatgatgtt acgaagttga atgacgttat tggaactatt catgaggcat caaacctacg
     5341 ttttttaatt agttgattgc aagtgtagcg gattcagacg cgaagaataa aatgctcttt
     5401 aacttgagat gggcgaaaaa ataggagtcc gagcttttgc aaatcgacgc ttacatgtat
     5461 atcagtttgt tctgaaagga ctagccaagc tttcatcata tctaaactcc aacgaatatc
     5521 gtccataatt attaccgtat cggaatgcat ataaggacga atttgttcaa aataccttaa
     5581 tgatgcttct aatgtatgat tcccatcgag atagagcatg tcaacttgtt tgagttgagg
     5641 tagcaatgaa ggaagaactt cttcgaagcg tcccgagtgg acagttatat tttgagcatt
     5701 aagatgtttg gcgttttgct gggcaagttt aactaagcta gggcgacctt caattgtgtg
     5761 tacttgtgcc gacgttggtt tagctaagta taaagctgaa atacctaatg aagtacctaa
     5821 ttcgagtatg gttttgggtt ggaggtaatt tgttaggcga aagagcaatc tgcctggttt
     5881 gggagaaagc aagctagatt gagcgattga gcgaaccgtt ttttggtgag gattgctttt
     5941 agagccagca cctgggtcgg aatcagaaat tagcgtgtta ttttcgagta gtttagtccg
     6001 tattttctcg attgcctcga aagcgtagta ttgattatcg ggcaggatga catctgtata
     6061 aagggaaaac acaaatggtg aatgaacccg attggggcct tttgcacgaa gccaataaga
     6121 gatgtataaa ccaattcgag ccagcatggg gcgtaaaagt agggtgattt gatgggaaag
     6181 ctaatggaaa aatggatggg aaatgttaca tttgggtgat agaaattgaa gttagaatgt
     6241 attggtaata ttgaacaatt gataaggtat atagttttga gtgcgtaaga aatgcgaatt
     6301 catgaattgt atgctgaagg aagttgaagc acatttgtat cgttaaaaaa aggaaacatg
     6361 aaaaagttat tgttcatttt tagtttgatg cttttaggac tggtgtcgaa agctcaagta
     6421 ggttcgaatg acctgatcat tcatggctac gttacaaatg cttcttctgg agctggtata
     6481 cccgatgtag aagtaacagt tatgg
//
LOCUS       DAOOQW010000127         6504 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_14500_length_6504_cov_5.375097, whole genome shotgun sequence.
ACCESSION   DAOOQW010000127 DAOOQW010000000
VERSION     DAOOQW010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 6504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6504
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14500_length_6504_cov_5.375097"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..700
                     /locus_tag="PK281_07815"
     CDS             <1..700
                     /locus_tag="PK281_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016200326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HOY49292.1"
                     /translation="ADQDAPSFGPCQQLDFELEMAFVTCRSNPLGTPVSIDEAEDYIF
                     GMVLFNDWSARDIQSWEYVPLGPFLAKSFASSVSAWVVSMDALEPFRVEGPVQDPKPL
                     PYLQFKGTHNYDIELEVLLETPRFNAARIVHSNYKYMYWNMVQQLTHQASNGCNIQVG
                     DLYASGTISGPIRDSYGSMMELTWRGTEPLKLPNNTERKFIQDNDTVIMKAYAKNEKV
                     RIGFGEVITTILPA"
     gene            752..1897
                     /locus_tag="PK281_07820"
     CDS             752..1897
                     /locus_tag="PK281_07820"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007095706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HOY49293.1"
                     /translation="MKLATKLIHAGQEPDPSTGAIMTPIYQTSTYVQEAPATHKGYEY
                     ARSQNPTRKALEDCLAAIENAAFGLSFGSGVAATDTVMKLLLPGDEVIAADDMYGGTY
                     RLFTKIYEPMGIRFHFVNMGNPENIRPLINDKTRLVWTETPTNPLMSITDIAAVAAIL
                     KGKNILLTVDNTFATPWLQNPLDLGADIVLHSGTKYLGGHSDLIHGCLMMNDPALREK
                     LYFIQKSCGAVPGPMDCFLVLRGLKTLHVRMKAHCENGRIIANHLRNHPKVGKVYWPG
                     FEDHPNHDIAKKQMRDFGGMMSFILKDESAEAARRFLTSTHLFSLAESLGGVESLVNH
                     PASMTHASIPKAERDRIGVVEGLIRLSVGIEDVEDLLADIDAALAKV"
     gene            1894..2604
                     /locus_tag="PK281_07825"
     CDS             1894..2604
                     /locus_tag="PK281_07825"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOY49294.1"
                     /translation="MKQAIITGTSRGLGKAFAEVLLEQGWNVLGISRTHSIDHESYYP
                     VEADLSKAGVASQLELPISPVADEFLLISNAATLGDIGYLGEISTSSIESSLMLNVIS
                     SSILINRFLAQTSNKKRTLISISSGASQNAYDGWGIYCSSKAAMDMLMDAIIKEREIR
                     NDSFTRIYSFAPGVIDTEMQEKIRSSNPDQFSKLERFQELKQNGQLASAVVVARQCIE
                     LLVNANTFSSGRYDIRNL"
     gene            2707..4383
                     /locus_tag="PK281_07830"
     CDS             2707..4383
                     /locus_tag="PK281_07830"
                     /inference="COORDINATES: protein motif:HMM:NF017722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004062 - aryl sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aryl-sulfate sulfotransferase"
                     /protein_id="HOY49295.1"
                     /translation="MSNFYLRILIGLSLFLIGINTQAQIVPANQSTLTYTDLVFEYPA
                     VENANYYVLSFKYKGHKQYFHQQVDSTQVSFCNLFRFGGNYTWNYQAFDKKGKSLYSS
                     PDYQFSITNTRSYDTLIYSNLIRGNYTGKGYVLFDQSKNMNTWDGKEVWRMPVLKNFP
                     LKGELNMRDLRMTRSGTFTTIITSSIAIEFDRDGNILWMAPESQLGTEKGKENYHHDF
                     RKLDNGNYMVMSETEEMVPLRVNKEMYKKLLGKDNIFEKNGKLFLMGVFGTLIEYSPD
                     NKIVWKWNCGEYLGERVLDGWLPESMHANAFYYDEVNKQLYYGLRDISLIMKVDKVTG
                     KVIDTFGSKLSPNQKNSSIGTFSLQHSIEKNKKGELLIFNNDSSEQAGVHSSLVILNE
                     TSNLKNILVKSVSCLIDSNDNGKALKYGSSEELPDGNILLGIGATGRTVVIDPRDNYR
                     IIQDIVMKSYVEKRWSPMVFYRSHFAPSLYPCYYSVNKSIGGITITNEGSEKDSYSIE
                     FILKDGSKKSFQSNIVYSDDTFFQAFDLSTVSAVHVISSANNRFKRSLAW"
     gene            4377..5384
                     /locus_tag="PK281_07835"
     CDS             4377..5384
                     /locus_tag="PK281_07835"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HOY49296.1"
                     /translation="MVKRVNTVLSIRQDYVLGILSFVFVNLILFSSSCSGNAPKEEAV
                     KKDTLTEYQPPDTSELKDDKFGQAVKYGAKLMTNTAYYIGPNGIAGHYLGNEMNCTNC
                     HLDAGAKTFGNSLYNTHRAYPQYRAREDKVLTSADRVNNCIERPHNGTPMPLDSYEML
                     AFVTYIQWLGKNYDPEKHYGHGLKEIDHKGKSSDPEAGRKVYEKHCQSCYLADGQGVY
                     DSNKHTYTYPPLWGEKSYQMGSSMHRVLKAASFIYHNMPRTTTITWDKPTLTVQEALD
                     VAAFVNSNDLHPRPNGGGISYPNLKTKPIDYYEGPFNDSYSVKQHTFGPWDGMVVGGA
                     K"
     gene            5468..5854
                     /locus_tag="PK281_07840"
     CDS             5468..5854
                     /locus_tag="PK281_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49297.1"
                     /translation="MNIFRKTILPILVATIWISISEFVRNEILAKSYWTSHYESLGLT
                     FPSEPINGAIWGLWSLFLAVGIFIISKKFSLIETTFLSWFMAFVLMWVVIGNMGVLPS
                     GLLYIAVPLSLLEAFLASFIIRKLAN"
     gene            5865..6302
                     /locus_tag="PK281_07845"
     CDS             5865..6302
                     /locus_tag="PK281_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49298.1"
                     /translation="MIQKSTAFVNVGISCLFISYWIIEIVQGISRSVWLPQLFLIIPS
                     FILIIPSIIHLISPSEKISKIYLSIAYWFVLLVAFIPACWIGILRMGEFKFFLVMNLI
                     TPICAILYLKNSKHQQLWKNEIFRFNFFAIVAQVYLLYLNVYL"
     gene            complement(6313..>6504)
                     /locus_tag="PK281_07850"
     CDS             complement(6313..>6504)
                     /locus_tag="PK281_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 domain-containing protein"
                     /protein_id="HOY49299.1"
                     /translation="TIFVELWGIDKGVYEYYNTLNSILGGAGPSVASPANPTSNITGG
                     AMGYFSAYTISRSWIIPHH"
BASE COUNT         2015 a         1110 c         1327 g         2052 t
ORIGIN      
        1 tgctgatcag gatgcgccta gttttggacc atgtcaacaa ctcgattttg agctcgaaat
       61 ggcattcgtt acgtgtcgat cgaatcctct tggaacgcct gttagtattg atgaagcaga
      121 agattacatt tttggaatgg tgctttttaa cgactggagt gcacgcgata ttcaaagctg
      181 ggaatatgtg ccactcggac cgtttttagc taagagtttt gcttctagtg taagtgcttg
      241 ggtggtgagt atggatgcat tagaaccatt ccgtgtggaa ggaccagttc aagacccgaa
      301 accattgcct tatctacaat tcaaaggaac acacaattac gatattgagt tagaagtgct
      361 attagaaact ccgcgtttca atgctgcacg aattgtacat tctaactata aatacatgta
      421 ctggaatatg gtacaacaac tcacccatca ggcttccaat ggttgcaaca ttcaagtggg
      481 tgatttgtat gcttccggca cgatcagcgg gccaattcga gatagttacg gttcaatgat
      541 ggaactcact tggcgcggaa cagaaccact taaactgccc aataatacgg agcgtaaatt
      601 cattcaggat aacgatacag ttattatgaa agcttatgcg aaaaacgaaa aagttcgtat
      661 tggttttggg gaagtaatta ctactatttt acctgcataa ggaatcggga tttttctgat
      721 ttactgagca tcaaccatca catttcaact aatgaaactc gctaccaaac tgattcacgc
      781 cggacaagag cccgatccaa gtaccggtgc aatcatgaca cctatttacc aaacttctac
      841 ctatgtgcaa gaagctccgg caacacacaa aggttatgag tatgcgcgtt cgcagaatcc
      901 aactcgtaaa gcgctggaag attgtttggc tgccatagaa aatgcggcat ttggcttaag
      961 tttcggcagt ggtgtagcag ctactgatac agtaatgaaa ttgttgcttc cgggtgatga
     1021 agtaattgct gccgacgata tgtatggtgg aacttaccgc ttgtttacca aaatttacga
     1081 gccgatggga atccgttttc actttgtgaa catgggaaat cccgagaata ttcgtccgct
     1141 tattaatgat aaaacacgct tggtttggac cgaaactcct accaatccct taatgagtat
     1201 aaccgatata gcggctgttg cagctatttt gaagggtaaa aacatccttt taacggtcga
     1261 taatacattc gcaacacctt ggcttcagaa tcctttggat ttaggagctg atatcgtgtt
     1321 gcattcaggt accaaatatt tgggtggaca cagtgatttg attcacggtt gcttgatgat
     1381 gaacgatccg gcattgcgtg aaaagcttta tttcattcaa aagagttgcg gtgctgttcc
     1441 cggaccaatg gattgttttc ttgttttgcg aggcttgaaa acattacacg ttcgtatgaa
     1501 ggctcactgc gaaaacggac gcatcattgc gaaccattta cgcaatcacc cgaaagttgg
     1561 gaaagtatat tggcctggat ttgaagatca tccgaatcat gacattgcta aaaagcagat
     1621 gcgtgatttt ggtggaatga tgagttttat tttgaaggat gaatcggctg aggcagcacg
     1681 taggttcttg actagcacac atttattttc gctagcagaa agtttgggtg gtgtggaatc
     1741 tttagtaaat catccagcat ccatgacaca tgcttctatt cccaaagctg agcgtgatcg
     1801 cattggagta gtagaaggtt tgattcgttt atcggttggg attgaagatg tagaagactt
     1861 gctagctgat attgatgcag ctttagcgaa ggtttgaaac aagcaatcat caccggaaca
     1921 tcgcgtggat taggcaaagc attcgcggaa gttttgttgg agcaaggctg gaatgtactt
     1981 gggatttctc gaactcattc gatagatcat gaaagctatt atccggttga ggcagatttg
     2041 tcaaaagctg gtgttgcaag ccaattagaa ttacctattt caccagtagc agatgaattc
     2101 ttgttgattt cgaatgctgc aacattgggt gatattgggt atttaggaga aatatccacc
     2161 agttcaattg agtcgagttt gatgttgaat gtgatatcat cgagcatttt aatcaatcga
     2221 tttttagccc aaacatccaa caagaaacgc acacttattt cgattagtag cggagcatca
     2281 cagaatgctt atgacggctg ggggatttat tgcagtagca aagcagcaat ggatatgtta
     2341 atggatgcaa ttattaaaga acgcgaaata cgaaacgatt cctttacccg aatatattct
     2401 ttcgcaccgg gcgtgattga tacggaaatg caggaaaaaa tacgatcgag taatcctgat
     2461 caattctcca agttggagcg ttttcaagaa ttaaagcaga atggtcagtt agcttcagca
     2521 gtagtagtcg ctcgacaatg catcgaatta ctggtaaatg cgaacacttt ttcaagtgga
     2581 aggtacgaca ttcggaatct gtagtttttt gatttaagga tcgtgctttc tatccggatt
     2641 tgcttgattt taacttgctc catttaaaat attatatttt tgctctgaat aaatatcatc
     2701 agtgttttga gcaatttcta cttacgcatc ttaattgggc ttagtttatt cttaatcggg
     2761 attaatacac aagctcagat tgttcctgcc aatcaatcta cactaacata caccgatctg
     2821 gtatttgaat atccggctgt agaaaatgcg aactattatg tgctttcttt taagtacaaa
     2881 gggcataagc aatactttca tcagcaagtt gattctactc aggtgagttt ttgtaatctc
     2941 ttccgatttg gagggaatta tacttggaat tatcaagctt ttgataagaa aggtaaatcc
     3001 ttgtattcat caccagatta tcaattttca ataactaaca ccagaagtta cgatacctta
     3061 atttactcga acttaattcg tgggaattat actggaaaag gatatgtgtt gtttgatcag
     3121 tcaaaaaaca tgaatacttg ggatgggaaa gaagtctgga gaatgcctgt attgaaaaat
     3181 ttcccactaa agggcgaatt aaatatgcgt gatttacgca tgacccgttc gggaactttc
     3241 acaacgatta tcacaagtag tatagcaatt gaatttgatc gggatggaaa tattctttgg
     3301 atggcgccag aaagtcaatt aggtacagaa aaaggcaaag agaattatca tcacgatttt
     3361 cgtaagctcg ataatggtaa ttatatggtg atgagtgaaa cggaagaaat ggttccattg
     3421 cgcgttaata aagagatgta caagaaatta ctgggcaagg ataatatttt tgaaaaaaac
     3481 gggaaattat tcttgatggg agtttttggg actttaattg aatattctcc tgacaataaa
     3541 atcgtttgga aatggaactg tggtgaatat ctaggagaaa gagttttaga tggttggttg
     3601 cctgaaagta tgcatgcaaa tgcattttat tatgatgaag taaataagca attatattat
     3661 ggcttaaggg atattagttt aattatgaaa gtggataaag tcacaggtaa agtgattgat
     3721 acatttggtt ctaagctttc ccctaatcaa aaaaatagtt caataggtac attctcttta
     3781 cagcattcta ttgagaaaaa taagaaagga gaattattga tttttaataa cgatagttcc
     3841 gaacaggcag gtgtacattc ctcattagta atattaaatg agacatcaaa tttgaagaac
     3901 attttagtga aaagtgtttc ctgtctaatt gatagtaacg ataacgggaa agcgcttaaa
     3961 tacggttctt cagaagaact tccggatgga aatattttac ttggtattgg tgctacaggt
     4021 cgaacagtcg taattgaccc aagagataat tatcgcatca ttcaagatat tgtcatgaaa
     4081 tcgtatgtgg aaaaacgatg gagcccaatg gtattttatc gtagccattt tgcaccatca
     4141 ttataccctt gttattattc agtaaataag tcaattggag gaatcaccat tactaatgaa
     4201 gggtctgaaa aggattccta ttcaatcgaa tttattttga aagacggttc taaaaaatca
     4261 tttcaatcga atatcgttta ttcagatgat accttttttc aagcatttga tctttcaaca
     4321 gtaagtgctg ttcatgtgat ttcttctgcc aataaccgtt ttaaaagatc gctagcatgg
     4381 taaaacgtgt aaatactgtc ttatctatca ggcaagacta tgttctaggt attttgtcat
     4441 ttgtattcgt taatctaatt ctcttttcat cctcttgttc aggcaatgcg cctaaagaag
     4501 aagctgtaaa aaaagatacg ttaaccgaat atcagccccc agatacaagt gaattgaaag
     4561 atgacaaatt tggccaagct gtgaagtatg gagctaaact catgactaat accgcctact
     4621 atattggtcc taatggtatt gcgggacatt atcttggtaa tgaaatgaat tgtaccaact
     4681 gtcatttaga tgcaggtgca aaaacgtttg gaaacagctt atacaatacc catagggcat
     4741 atcctcaata ccgagcaagg gaagataaag tacttacaag tgcagatcgg gtaaataact
     4801 gcatcgaacg ccctcataac ggaacaccta tgccacttga ttcctatgaa atgttagctt
     4861 ttgtaactta tatacaatgg ctaggaaaga attacgatcc tgaaaaacat tatggtcatg
     4921 gcttaaaaga gatcgatcat aaaggaaaaa gttccgatcc agaagctggt agaaaagtct
     4981 atgaaaaaca ttgtcaatcg tgctatttag ccgatggtca aggcgtttat gactcaaata
     5041 agcatactta tacctatcct ccactttggg gtgagaaatc ctatcaaatg ggttcaagta
     5101 tgcatcgtgt gctaaaggct gcatctttta tttatcacaa tatgcctcga acaacaacca
     5161 ttacttggga caaacctact ttaactgttc aagaagcatt ggatgttgct gcttttgtta
     5221 attcaaatga cttgcatcca agaccaaatg gaggtggcat ttcttatcca aatttgaaaa
     5281 ccaagccaat tgattattac gaaggtccat tcaacgattc ctattcagta aaacaacaca
     5341 catttggtcc ttgggatggt atggttgtag gcggggcgaa gtaattcatg gaattgttta
     5401 gtgtgtttga aatgctatat caatagctat gattaagtac acatcagtaa aaaacaattg
     5461 aattggtatg aacattttcc gaaaaactat tttacccata ctagtagcaa ctatttggat
     5521 tagtatttcc gaatttgttc gtaacgagat tttagcaaag tcatactgga caagtcacta
     5581 cgaaagttta ggattgacat ttccatcaga accaataaat ggtgctattt ggggattatg
     5641 gtcattgttc ttggccgtgg gaatttttat tatttctaaa aaattcagct taattgaaac
     5701 aaccttttta tcctggttta tggcctttgt actgatgtgg gttgtaattg gtaatatggg
     5761 agttttgcct tctggattgc tttatatagc tgttccactt agcttattgg aagcattttt
     5821 agcatcattc atcatccgta aattggcaaa ttaaatctga taaaatgatt cagaaatcaa
     5881 cagcatttgt caatgttgga atttcttgtt tatttatttc ttattggata attgaaatcg
     5941 ttcagggtat ttcaagaagt gtttggttgc ctcaattatt tttaattata ccttctttca
     6001 ttcttataat tccttctatt attcatctga tatcaccttc agagaaaata agtaaaatct
     6061 acctttccat cgcatattgg tttgtattac tagtagcatt cattcctgct tgttggatag
     6121 gaatattacg aatgggtgag tttaaattct ttcttgtaat gaatttaatt actcctattt
     6181 gtgctatttt atatttgaaa aatagtaaac atcaacaact ttggaaaaac gaaatattcc
     6241 gcttcaattt ttttgcaatt gttgcccagg tataccttct atatctgaac gtttatttat
     6301 agagaatgcc tattaatggt gtggaatgat ccaacttctg gaaatagtat aagccgaaaa
     6361 atatcccatt gcacctcctg ttatattact tgttggattg gcgggtgaag caaccgatgg
     6421 accagctcct ccaagtatac tatttaacgt gttgtaatat tcataaacac ctttatcaat
     6481 tccccaaagc tcaacgaata tagt
//
LOCUS       DAOOQW010000128         6385 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_14836_length_6385_cov_6.252765, whole genome shotgun sequence.
ACCESSION   DAOOQW010000128 DAOOQW010000000
VERSION     DAOOQW010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 6385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6385
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14836_length_6385_cov_6.252765"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="PK281_07855"
     CDS             complement(<1..518)
                     /locus_tag="PK281_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49300.1"
                     /translation="MRNLLVLLILVLSNQLFSQRFDWATTGGATGIANSFYGAVDIAR
                     DPQGNIYTFDYSNHAQQCQGDTLQPYDGITAYIYKFNSQGELLFMNRVGELGGSFTPF
                     NIETDDAGSLYILGQGAGNGAIIVKEDTVPAVDFTNQLIKLDSNGNFVWKQNTGVSTN
                     NQGCMLQHSGGYL"
     gene            complement(558..2186)
                     /locus_tag="PK281_07860"
     CDS             complement(558..2186)
                     /locus_tag="PK281_07860"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY49301.1"
                     /translation="MKKVVFLLLIFPSVLLAQINQIWHTDRPYSFSFNDSYMRIVNSD
                     IYTHSSIFEGGSWQSAQSITTDGIENWYMEHDQFQACTNCGNAGVESVAIASNGDAYG
                     VGFQPSSPYGGRYVYKTDAGGNLIYAEEYFTSTFSGEFNDVKLSNDETKLFVFGEMYA
                     EAYNTIVPHLFKLNAATGAIENTVMTAPIGWTGVKSLGVDAGDNLYMNSSDLDTLNYS
                     SWDSDFNLRWYRQIPVAGYQGSGQVQTLFYSNGDVLFVTFLNNYNTNDDRRLHLARYT
                     TTGDLIWQTTHLLEDQDIYGYLYRDVALDVNGNVDIFFAKTKFVGTGGGFGGEDFGGK
                     GGETQIRPAIYKFNSAGVYQWNYVFEGNSGVEFSSEYPARMVVDENGYVIGSSFGNSS
                     GYAGISLFIINPQGLLETSLFQNGSIYSAIKGMVYEGNRTFYTHGIGAIEGTFTADRW
                     TLARYHYDYTTGVKAVSNEGFIVFPNPVKNSHTINIKGLKMNDRIEIRDIQGRLIKEI
                     SHSGVTIETIDITGISQGIYYVKAGISSQKLIIQ"
     gene            complement(2316..2648)
                     /locus_tag="PK281_07865"
     CDS             complement(2316..2648)
                     /locus_tag="PK281_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49302.1"
                     /translation="MKTILSLLFIGLIGLSTSHAESNDNKKSNRLKKEVRKELIQLFN
                     ERNLDRPTHKGSVVALVKVDSQGKGLVMEMDSEDQQLQSYVKKQVESRNFQNLKNETI
                     RLVVDFRN"
     gene            2890..3873
                     /locus_tag="PK281_07870"
     CDS             2890..3873
                     /locus_tag="PK281_07870"
                     /inference="COORDINATES: protein motif:HMM:TIGR02128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoglucose/phosphomannose
                     isomerase"
                     /protein_id="HOY49303.1"
                     /translation="MKDLIAGFPQQVRDALNIFKAQQLKPLPRKATSVLISGLGGSGI
                     GGSIVAELCAPQASIPVQVSKDYSIPAWVGPETLVIISSYSGNTEETLQAMQAAISQK
                     AMIVCVTSGGEVLEQAKKHQFDAIVIPGGNPPRACLGLSLTQLCGILEHYEIATGLLA
                     EIALGIDLIEKNAISIQHEAESIARAISFSLPVIYSSIGYEGVAIRFRQQLNENAKML
                     CWHHVVPEMNHNELVGWVDKAPKIAAIFLRNDDEYYRTAKRIDICKGIVYNLAGKTIE
                     LRSKGESPLQRMIWWINLGDWISWFASVERNVDAIEINVINRLKNELSNLQ"
     gene            3870..4592
                     /gene="lipB"
                     /locus_tag="PK281_07875"
     CDS             3870..4592
                     /gene="lipB"
                     /locus_tag="PK281_07875"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013070188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HOY49304.1"
                     /translation="MIYQQPNRDISIIDLGLIGYQEAWDFQEKMFAELLALKKENRDA
                     TNPVNVPNYLILCEHPPVYTLGKSGKEENLLLSKEKLEQIGATYFKINRGGDITHHGP
                     GQLVVYPLLDLENFFTDIHLYLRKLEEAVIQTLFTLGIQAGRYPGYTGVWIQPEDLSA
                     RKICAMGVKCSRWVTMHGIALNVNNDLSYFNHIIPCGIEDKAVASIQQELPGFPISME
                     LVKQLFLKNFEKEFDAHLLNNQ"
     gene            4638..5444
                     /locus_tag="PK281_07880"
     CDS             4638..5444
                     /locus_tag="PK281_07880"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HOY49305.1"
                     /translation="MKTRLFVISLFFLGSLSAQNSRTHTNRAFKRGEVLSYRVHYGLV
                     DAGEATVAIAQDSLKFNKSSTFHLVGTGKSRSAFEWFYKVRDRYDSYIDEETLLPHLF
                     LRRVDEGGFIINQDYKFNQSQNTVNVKRGGSDEARSTPGKQFTVPTSTHDILSAFYYA
                     RNMNLEGVKMGDIITIQTFFDEEVFPMKIKLIGRETLNTRAGKIRCIKIHPLIQQGRV
                     FKEEDDLTLWVSDDLNRIPVRIQAEVLVGSIKMDLKSYANLANPLALESK"
     gene            5736..6245
                     /locus_tag="PK281_07885"
     CDS             5736..6245
                     /locus_tag="PK281_07885"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOY49306.1"
                     /translation="MRYKSNTVTVAEFNQSVDQFSDNIYRFILKNMRDEDEARDVVQD
                     TFEKVWLKASEVSFEKAKSYLFTTAYRIMLERIRKKKRQEPIDNSLRVVAGSSKDPEF
                     DLKRVLNEAIAKLPEDQRSVIMLRDYEGYSYEEIGRITGLNESQVKVYIFRARVFLKN
                     YLVSAEVVL"
BASE COUNT         1982 a         1257 c         1202 g         1944 t
ORIGIN      
        1 agatatccac cgctatgttg aagcatacag ccttgattat tggttgaaac gcctgtgttt
       61 tgtttccaaa caaaatttcc attgctatcc aattttatta attgatttgt gaagtcaact
      121 gctggcacag tgtcttcctt tactatgata gccccattac cggcaccctg accaagtata
      181 tatagacttc cggcatcatc ggtttcaata ttgaacggag taaagctgcc tccaagttcg
      241 ccaactctgt tcataaacaa caattcacct tgcgaattga acttatatat ataagcggtg
      301 atgccatcat atggttgcaa tgtatcgccc tgacattgtt gggcatgatt gctataatca
      361 aaagtgtaaa tgttaccctg tgggtcgcgc gcaatatcaa cggcaccgta aaaggagttg
      421 gctatgcccg ttgcccctcc tgtagttgcc caatcgaaac gttgagagaa taattggttg
      481 cttaaaacaa gaattaacaa tactaatagg tttctcatag taggttttta gctcgatgaa
      541 aaattgattt ttgtacttta ttgaatgatt aacttctgac tagaaattcc tgcctttaca
      601 tagtatattc cttgtgagat tccagtaata tcaatagtct caatagttac tcctgaatga
      661 gaaatctctt taatcaatct tccttggata tcacgtatct caattcgatc attcattttt
      721 aaacccttga tattaatcgt atggctgttt ttaaccggat tgggaaaaac aataaatccc
      781 tcatttgaaa ccgctttaac acctgtggta taatcataat gataacgggc taaagtccat
      841 cggtcggctg taaaagtgcc ttcaattgca ccaattccat gcgtataaaa cgttctattt
      901 ccttcgtaga ccattccctt aatagctgaa taaattgagc cattttggaa cagcgatgtt
      961 tctaataaac cttgcggatt gataatgaat agagatattc cagcataacc tgaggaattt
     1021 ccaaaagaag aaccaatcac ataaccattt tcatctacta ccattcttgc tggatattca
     1081 gaagaaaatt caactcctga attgccttca aatacatagt tccattgata aactccagca
     1141 ctgttaaatt tatagatggc tggtctaatc tgagtttcgc caccttttcc tccaaagtct
     1201 tcacctccga aacctccacc agttccaaca aacttagttt ttgcaaaaaa aatgtccaca
     1261 ttaccattta catccaaggc aacatcacgg tataaataac cataaatgtc ttggtcttca
     1321 agtaaatggg tggtttgcca tattaagtct cctgtagtag tgtatcgtgc aagatgtaag
     1381 cgccgatcat cattcgtatt gtagttattt aaaaaggtaa cgaaaagtac atctccattc
     1441 gagtagaata atgtctgaac ctgtcccgaa ccttgatatc cagctactgg gatttgtcta
     1501 taccacctta gattgaaatc agaatcccag gatgaataat tcaacgtatc taaatcggaa
     1561 gaattcatat ataaattatc tcccgcatca acaccaagcg attttacccc tgtccaacca
     1621 ataggtgcgg tcataacagt attttcgatt gccccagttg ctgcatttaa tttaaataga
     1681 tgaggaacaa ttgtattgta agcttcggca tacatttccc caaacacaaa caattttgtt
     1741 tcatcattcg acagcttgac atcattaaat tctcccgaaa aagtggatgt gaaatattct
     1801 tccgcataaa tcaaatttcc accagcatca gttttataca catagcgtcc tccgtatgga
     1861 gaggaaggtt gaaatccaac tccataagca tcaccatttg aggcaattgc taccgattca
     1921 actcctgcat ttccacaatt ggtacaagct tgaaactgat catgctccat gtaccagttt
     1981 tcaattccat cagttgttat tgattgagct gattgccaag aaccaccctc aaaaatggaa
     2041 gaatgagtat agatatcgga atttacaatt cgcatatacg aatcgttgaa tgaaaagcta
     2101 taaggcctat cagtatgcca gatctgatta atctgagcca gtaatacact tggaaatatc
     2161 aataaaagaa atacaacctt tttcatgtga aatgcccgaa aataaaggca aaggtccgtt
     2221 atactgaggc attgtcaatg actaatatta gctataaaaa aagccaccct cttaacgagg
     2281 atggcttaac gtggatatat aaagaacgag agtatttaat ttctaaaatc aaccaccaat
     2341 cggatggttt catttttcag attctggaag ttccgacttt caacttgttt cttaacatat
     2401 gattgaagtt gctggtcttc tgaatccatt tccattacta aacctttgcc ttgagagtca
     2461 acttttacaa gtgcaacaac agatccttta tgtgttggtc gatctaagtt gcgttcatta
     2521 aataattgaa tcaattcctt tctaacctcc ttttttagac gattactttt tttattgtcg
     2581 tttgattccg catggctagt tgataatcct atcaaaccaa tgaacaaaag ggagagaatc
     2641 gttttcataa tttctgggtt tttagagcat ttgcagataa gtgttctttc ctagcatttt
     2701 ggttacagca catttgaaca aataatcgca atagtaacat tgtcaagcat ttaaaattag
     2761 tggcggttta ttttgcgaag cgagtttccc cgtctgacac aagaatctca attcacatat
     2821 gaattctgta agcattttca gatatcaatt ctcaaatacc acgtatttta gccacccgat
     2881 attttacgaa tgaaagatct cattgccgga tttcctcaac aagttcgaga tgcattaaac
     2941 atctttaaag cacaacaatt aaaaccgctt ccccgaaaag caacttctgt tcttattagt
     3001 ggattaggtg gttcgggcat tggtggaagc attgtagctg aattatgtgc acctcaagca
     3061 tccattccag ttcaagtatc gaaagactat tcaattcctg cttgggttgg tccagaaacg
     3121 cttgtaatca tttcttccta ttctggaaat accgaagaaa cacttcaagc catgcaagcc
     3181 gccatctcac aaaaggcaat gattgtgtgt gttacatcgg gtggggaagt tctagagcaa
     3241 gctaaaaagc atcaatttga tgcgattgtt attcctggcg gaaatcctcc tcgtgcttgt
     3301 ctcggtttat cactcactca actgtgtggt attttagaac attatgaaat tgctacagga
     3361 ttattagctg aaattgcact aggaatcgac ctcattgaaa agaatgcaat aagcatccaa
     3421 catgaggcag aatctattgc acgagctatt tcatttagtc ttcctgttat ttattcaagt
     3481 ataggatacg aaggtgttgc catacgtttt cgccaacaac tgaacgagaa cgctaaaatg
     3541 ctttgctggc atcatgtagt tcccgaaatg aatcacaacg aattggttgg ttgggttgat
     3601 aaagcaccaa aaattgctgc cattttcttg cgcaatgatg atgaatacta tcgcactgcc
     3661 aaacgtatcg acatatgcaa gggcatcgtt tataaccttg ctggtaaaac aatcgaactt
     3721 cgttcaaaag gcgaaagtcc gctgcaacgt atgatttggt ggattaatct gggcgattgg
     3781 attagctggt ttgcttctgt cgaacgtaat gttgatgcaa ttgaaattaa cgttatcaac
     3841 cgactgaaaa acgagttgtc taacctccaa tgatctatca gcagcctaat cgcgacattt
     3901 caatcatcga tcttggccta attggatatc aggaggcttg ggattttcaa gaaaaaatgt
     3961 ttgccgaatt gctagccttg aaaaaggaaa atcgagatgc aactaatcca gtcaacgttc
     4021 ctaattattt aattctttgc gagcatcctc ctgtttacac acttgggaaa agtggaaaag
     4081 aggagaattt acttttgtcg aaggaaaagc ttgagcaaat tggagcaacg tattttaaaa
     4141 taaaccgcgg tggagatatt acccatcatg gtcccggtca attagttgtt tatcctttgc
     4201 tcgatctgga gaatttcttc acagatatac atctatactt aagaaaactg gaagaagcag
     4261 ttattcaaac gcttttcaca ttaggaattc aagcgggaag atatccagga tatacaggag
     4321 tttggatcca acccgaagat ttatcggcgc gtaaaatttg tgctatggga gttaaatgta
     4381 gccgttgggt taccatgcac ggaatcgcgc tcaacgtgaa taatgatcta tcctatttca
     4441 accacatcat tccttgtggt attgaagata aagcggttgc cagtattcaa caagaacttc
     4501 cagggtttcc aatttcaatg gaattagtca aacaactttt cttaaagaat ttcgagaaag
     4561 agtttgatgc tcatttactg aataaccaat aaaatcaagt ggcatagcgt ttgtcttgcg
     4621 tcgacaaaac ctatcgtatg aaaacccgct tgtttgttat tagccttttc ttcctcggtt
     4681 cactttctgc acaaaattct agaacgcaca ctaacagagc ttttaaacga ggcgaagtgc
     4741 tttcgtatcg tgttcactac ggattggtag atgccggaga agcaacagtt gcaattgctc
     4801 aagacagttt gaaattcaat aaatcgtcta ctttccattt agttggaacg ggaaaatcaa
     4861 gaagtgcttt tgaatggttt tataaagttc gtgatcgcta cgattcttat atagatgaag
     4921 aaacgctact tccacatttg tttctacgtc gcgtcgatga aggtggattt atcatcaatc
     4981 aggattataa attcaaccaa tctcaaaata cagtgaacgt taagcgcgga ggatctgatg
     5041 aagctcgcag tacaccggga aaacagttta ccgttcccac ttcgacacac gatattctct
     5101 cggctttcta ttatgcccga aatatgaatt tagaaggtgt taaaatgggc gatatcatta
     5161 ccattcagac cttttttgat gaggaagttt ttcccatgaa aatcaaactt attggacgtg
     5221 aaacgttaaa cacacgagct ggtaaaatca gatgtatcaa aatccatcct ttaattcaac
     5281 aaggacgtgt gtttaaagaa gaagatgacc ttacactttg ggtcagcgat gatctaaatc
     5341 gtattccagt tagaattcaa gccgaagtgc tggttggatc aatcaagatg gatttaaaaa
     5401 gctatgctaa cctcgccaat ccattggcac ttgaatctaa ataatcacga ttaaattcaa
     5461 gaatcctgac taacacaatt agttgggatt ttttttgggc ggtcgagcgg gctattactg
     5521 caaatccttc cgcaacaata ttcattaaat aatcaatcat ccaccagcgt cagggttttc
     5581 cgctgcttgt cccaatgggg ctatccctac cgctttttgc taaatggaac ttcacgagaa
     5641 aaatgtgaat tcacgaaagg ctacagcagc ttgattttta gaggaattga aaaaaatgcg
     5701 attcactgta acttagtcgg cagtttgtgc ctctcatgag gtataaaagc aataccgtga
     5761 ctgtagccga atttaatcaa agtgtagatc agttctctga taacatctac cgcttcatcc
     5821 tgaaaaatat gcgggacgaa gacgaagcac gtgatgtggt gcaggataca tttgaaaaag
     5881 tttggcttaa agcatccgaa gtttcttttg aaaaggccaa atcgtatttg tttactaccg
     5941 cttatcgaat catgttagag cggattcgta aaaagaaacg tcaagaaccc attgataatt
     6001 ccttacgagt tgttgcagga agttcgaaag atcctgaatt tgatttgaaa cgtgtattga
     6061 atgaagccat tgcaaaactg cccgaggatc agcgctccgt aattatgttg cgtgattatg
     6121 aagggtattc ttatgaagaa attggacgca taaccggact taatgaaagt caagttaaag
     6181 tgtacatttt cagagcgcgt gtgtttttga aaaattactt ggtaagtgcg gaggtggtgt
     6241 tatgaaaaag atcgatttta ccaattacga agcatggctt ctcgattatg ccgagggcaa
     6301 tctcagcgct gaaaatacag ctgagctttt gttgtttttg gaacaacatc ccgaactaaa
     6361 aatcgatgta gaaggcttaa tggaa
//
LOCUS       DAOOQW010000129         6340 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_14968_length_6340_cov_5.818934, whole genome shotgun sequence.
ACCESSION   DAOOQW010000129 DAOOQW010000000
VERSION     DAOOQW010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 6340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6340
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14968_length_6340_cov_5.818934"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1099
                     /locus_tag="PK281_07890"
     CDS             <1..1099
                     /locus_tag="PK281_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49307.1"
                     /translation="INGDNFRIRVVCESPAMVSPDNGYPVHIHTSVFPSVSLEISPES
                     ICGSDPVMASISGNGLGTSPIYTWSLNGTPISLESPSIVLDTLSVNTLIAVSISSSLT
                     CANPIINTVSQSLTVAESPDAFAGNDTSICLGETIQIGSLISDSFTWFPTSGLDNPTN
                     AQPIASPDQNVSYVLTVTNTAGCSKTDTISIQVNNLPATPTMFQTAGLLVVEGDLPGT
                     FNWFLDGNPIADESNDSLVISSTGSFTVQWIDPNGCSSELSNSIVIGTVGLEENLSNP
                     VITCLPNEWRISGLAENTERFYYSLFDVSGRMMVQGSAQEDGQGTIRIEPISLASGMY
                     FLRVIGLANQQETSTQDIRNNQGFTFKLIRN"
     gene            complement(1252..1324)
                     /locus_tag="PK281_07895"
     tRNA            complement(1252..1324)
                     /locus_tag="PK281_07895"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1289..1291),aa:Gly,seq:gcc)
     gene            complement(1385..3964)
                     /gene="mutS"
                     /locus_tag="PK281_07900"
     CDS             complement(1385..3964)
                     /gene="mutS"
                     /locus_tag="PK281_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOY49308.1"
                     /translation="MKQYNEIKAKYPDALLLFRVGDFYETFGEDAVRAAGILGIVLTR
                     RANGAAQYIELAGFPHHSLDTYLPKLVRAGLRVAICDQLEDPKATKTIVKRGVTELVT
                     PGLATSDKLLDYRSNTFLAAVVPGKEEVGLAFLDHSTGEFMIAQGKPAYAEKLIQNFQ
                     PGEVLMPKTAAKGFKEIHGERFFSYPLDEWIFQETYSRELLQKQFDTISLKGFGVEGM
                     PLAIIAAGACLHYLNETNHDKTAHITAVSRIEEDKFMWLDGFTIRNLELFSSAYEQAM
                     TLFDVINHTLTPMGARMLKRWLALPLKDLRPIQDRLQLVDLLRHTPELAVQLSESLKQ
                     IGDLERIIAKVAVQRVNPRELKQLQRALYAIAEVQQYCAASVDPLLQRIGEQLNPCLL
                     IRQRIENEISDEAPVALHKGDVISDGVDAELDELRDIKRSGKDYLLKIQQREIEATGI
                     TSLKISFNNVFGYYLEVTNSHKDKVPETWIRKQTLVNAERYITPEIKVFEEKILGAED
                     RIAGIESRLYQALLSAIAEYIQPIQHNAFLLAQIDCLRGFADLAERNNYCRPEVNDGF
                     ALEITDGRHPVIEKQLGIGVEYVANDVLLDPDGVQLMMITGPNMSGKSALLRQTALIT
                     IMAQIGCFVPASAAKIGLVDKIFTRVGASDNISSGESTFMVEMNETASILNNLSDRSL
                     ILLDEIGRGTSTYDGISIAWAIAEFLHEQPVNRAKTLFATHYHELNDMAARFPRIRNF
                     NVSVKEVNQKVIFLRKLKEGGSNHSFGIHVAKMAGMPRKLVDRATEILHELEKLHRKG
                     EANVSPKVPDSVQTGMQLSFFQLNDPVLENIKEELEKTDINNLTPMDALLKLNEIKRL
                     LGG"
     gene            4051..>4286
                     /locus_tag="PK281_07905"
     CDS             4051..>4286
                     /locus_tag="PK281_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HOY49309.1"
                     /translation="MRKLSITELNRKHPEDFRQSKKRPFRIVLDNVRSAHNIGSVFRS
                     ADAFAVEQILLCGITAVPPNREIHKTALGATETV"
     assembly_gap    4287..4386
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4387..>6340)
                     /locus_tag="PK281_07910"
     CDS             complement(<4387..>6340)
                     /locus_tag="PK281_07910"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOY49310.1"
                     /translation="DFTINQPDVLQAGIETISSVCGLANGSMNGSAFGGTSPYQYSWS
                     TGGNQAQITGLTSGSYTLQVTDAHGCQSALTKSFQDPGAPVVSLIQAQNISCFGGADG
                     AIEISAQGGSGLLSLNWSNSNLLGASINGLAAGNYTLVVSDELNCKDTLLTNLSQPDA
                     LISSHTIENVRCFGGTDGAAHLIINGGTQPYSFLWSNSTSMYAGQADLNQVSAGAYQV
                     QITDAHACQIVDSITISEPELLIGSMHAQAATCFGSATGMLLSQANGGVLPYEYAWSN
                     GSQSPNADNLAAGIYSVLITDANGCGISLQGEVLQPQELIVQFSGERALCKGDSSLLT
                     ASATGGTLPFSFNWSGGETQDQIVWNALETHEVQVIVQDSKGCTDTLKTQLLVYELPR
                     VEVLSAQTSICAGTCAEFSAAAVPGGSYVWSDSHNMIHTGQSVSFCYDDAANVGITLQ
                     VTDEHGCLNQWTADSYLTVHPLPEIGFEAEQYTVPLLEALVHFENTTSGALSYIWDFD
                     PRMDEQTSNDISPSYQFTEIGEYPVSLIATNEFGCSIQLQKTITVTEDFAVYFPNAFT
                     PNNDGSNDRFLPVGVGIQSVGFSMQIYNRWGNLVYETKNLTEGWDGNYESKINQGESA
                     SEVFVWQVSLTDFKGLVRDYRGSVTL"
BASE COUNT         1810 a         1317 c         1299 g         1814 t
ORIGIN      
        1 tattaacggt gacaatttcc gaattcgtgt agtttgtgaa tcaccagcga tggtttcacc
       61 cgataatgga tatcctgtcc acattcacac ttctgttttc ccttctgtat ctctagaaat
      121 tagcccagaa agtatctgtg gttccgatcc ggtaatggca agtattagtg gaaatggatt
      181 aggaactagt cctatctata cttggtcatt gaatggtaca cctatctcat tagaatcacc
      241 aagcattgtg ctggatacat tgagcgtaaa tacactaatt gctgtttcta tcagtagcag
      301 tttaacctgt gcgaatccaa tcattaatac tgtgtcacaa tcgctaactg tagctgaatc
      361 gcctgatgct tttgcaggaa atgatacatc tatttgttta ggagaaacga tacaaattgg
      421 atccttgata agtgattcat ttacttggtt tccaacaagc ggactagata atcctacaaa
      481 tgcacaaccc attgcatctc cagatcaaaa cgttagttat gtactaaccg ttaccaatac
      541 ggcaggttgt agcaaaacag atacgattag tattcaagta aacaacttac cagcaacgcc
      601 cactatgttt caaacagctg gattgcttgt ggtggaagga gatttaccgg gtacgtttaa
      661 ttggttttta gatggtaatc caatagcaga tgaatcgaac gactctttag ttataagttc
      721 aactggtagt tttacggtac aatggattga tccaaatggt tgttcatcag aactttcgaa
      781 tagtattgtg attggtacag ttggattaga ggagaatctt tccaatccag taattacttg
      841 ccttccgaat gaatggagaa taagtggatt agctgaaaat acggaacgtt tttactattc
      901 cctttttgat gtaagcggta gaatgatggt tcagggttca gctcaagagg atggtcaagg
      961 aacaattcga attgaaccga tttctttagc aagtggaatg tattttcttc gagtgattgg
     1021 attagctaat cagcaagaaa cttcgacaca ggatattcgc aataatcaag gctttacctt
     1081 taagttgata cgaaactaaa agctgcgtga atgatgggaa tggatgaggc gaagccgaaa
     1141 tgcccgaagg gccggagcga agcgtagcca tggacagcat gaccgaggtt tttaccgagg
     1201 gcacgcccaa aaaaagaaat aaaaaaagcc tccagatttt ggaggcttta gagcgaaaga
     1261 cgggattcga acccgcgacc ccgaccttgg caaggtcgtg ctctaccaac tgagctactt
     1321 tcgcgtaggg gatggcaaaa gtaaaataaa atcgttttca ccaaaacatt ttcttgtatt
     1381 tttcttatcc gcctagtaaa cgcttgattt cgttgagttt taaaagcgca tccatgggtg
     1441 taaggttgtt gatatcggtt ttttcgagtt cttctttgat gttttcgagc acaggatcgt
     1501 tcaattggaa gaaacttaac tgcattccag tttgcaccga atcgggaact ttgggggaaa
     1561 cattcgcttc tcctttgcgg tgaagttttt cgagttcatg caaaatttcg gtagcccgat
     1621 cgaccaattt acgcggcatt ccggccattt tagccacatg aataccaaaa ctgtgatttg
     1681 aaccaccttc cttaagcttt cttagaaata tgactttttg attgacttcc ttgactgaaa
     1741 cattgaagtt tcgaatacgc ggaaaacgag ccgccatatc gtttagctcg tggtaatgtg
     1801 tcgcaaatag tgttttagca cggttcacag gttgttcgtg aagaaattca gctatagccc
     1861 aagcaatgga aattccatcg taggtactgg ttccacggcc aatctcatcg agcaaaatca
     1921 aacttcgatc gctgagatta ttgaggatcg aagctgtttc attcatctcc accatgaatg
     1981 tggattctcc gctgctaata ttatcggaag ctccaactcg ggtgaaaatt ttatctacca
     2041 gtccaatctt tgctgctgag gctggcacaa aacagccaat ctgagccata atagtaatca
     2101 aggctgtttg tcggagcaat gccgatttac ccgacatgtt ggggccggtt atcatcatga
     2161 gttgcacacc atcgggatcg agtagtacat cgttggcaac atattctact ccaataccta
     2221 attgcttttc aataacagga tgacgtccgt cggtgatttc aagggcgaaa ccatcgttta
     2281 cttcggggcg acaatagtta tttcgttcgg ctaagtcggc aaatccgcgc agacagtcga
     2341 tttgagctag caagaacgca ttatgttgaa tcggttgaat gtactcagct atagcagata
     2401 aaagtgcttg atataagcga gattcaattc cagctatacg atcttcggca cctagaattt
     2461 tctcttcaaa taccttgatt tctggagtga tgtagcgttc ggcattgacc aacgtttgct
     2521 tacgaatcca ggtttcggga actttatctt tatgcgaatt ggttacttcg aggtagtatc
     2581 caaaaacgtt attgaagcta attttgagtg aggtaattcc tgtggcttcg atttcgcgct
     2641 gctgaatttt aagcagataa tcttttccgg aacgtttaat atcgcgcagt tcatccaatt
     2701 cggcatccac accatcgcta atcacatcac ctttatgcaa ggcaactgga gcttcgtccg
     2761 aaatttcgtt ttcgatgcgc tgacgaatga gcaaacaagg attgagttgt tctccaatac
     2821 gttgtaatag tggatcaacg ctcgcagcac aatattgctg aacttctgct atggcataaa
     2881 gcgcacgctg taattgcttt aactcacgcg gattaacacg ttgaacggca actttggcaa
     2941 tgattcgttc aagatcacca atttgtttga gtgattcgga taattggact gctaattcgg
     3001 gcgtatgtct taatagatcg accaattgta gacgatcttg tataggacga agatctttga
     3061 gcggtaatgc caaccaacgt tttagcatac gcgctcccat cggtgtaagc gtatgattga
     3121 taacatcgaa caaggtcatg gcttgctcgt aggccgaaga aaaaagctcc agattacgaa
     3181 tggtaaaacc atcgagccac atgaacttat cttcttcgat gcgcgaaaca gccgtgatgt
     3241 gagctgtttt atcgtgattc gtttcgttca aataatgtaa gcaggcacca gcagctataa
     3301 tagccaatgg cattccttct acaccaaaac ctttgagtga aatggtatcg aactgcttct
     3361 gaagtaattc ccgcgaataa gtttcttgaa aaatccattc atcgagcgga taggagaaaa
     3421 agcgttctcc gtggatttct ttaaagcctt tcgcagccgt ttttggcatt aagacttcgc
     3481 ccggttggaa attctgaatg agtttttcgg cataggcagg tttgccttga gcaatcataa
     3541 actcaccagt ggaatggtcg agaaaagcca atccaacttc ttctttccca ggaactactg
     3601 cagctaagaa tgtattacta cggtaatcga gaagtttatc gcttgttgct aaaccaggag
     3661 ttactaattc ggtaacacct cgtttaacga tggttttggt ggctttggga tcttcgagtt
     3721 gatcacaaat agcaacacgt aatccagcac gaaccaattt aggtaaatag gtatcgagtg
     3781 aatgatgcgg aaaaccggct agttcgatgt attgagctgc accatttgca cgacgcgtga
     3841 gcacgattcc taatatccca gctgcacgaa cagcatcttc tccgaaggtt tcgtaaaaat
     3901 ctcccacacg aaataatagc aacgcatccg ggtatttcgc cttgatctcg ttgtactgtt
     3961 tcatcaaagg agtctccacg acgccttttg catcgactgt ttttgctgct ttcaaaggga
     4021 tttggtgtaa tttggtccca tctcagaacc atgcgaaagt taagcattac cgaattaaac
     4081 cggaagcatc ccgaggattt ccgtcaaagt aaaaaacgtc catttagaat agtgcttgat
     4141 aatgtgcgta gtgcgcataa tatcggctct gtttttcgct ccgcggatgc ctttgcagtg
     4201 gaacaaattc ttctttgtgg catcacggct gttcctccca atcgggagat tcataaaaca
     4261 gcacttggag ctaccgaaac ggtcgannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4381 nnnnnnataa gcgttaccga gccacgataa tcccgaacga gtcctttgaa atcggttaaa
     4441 ctcacttgcc aaacaaaaac ttcagaagct gactcacctt ggttgatttt tgattcataa
     4501 ttcccgtccc aaccttccgt aagattcttc gtttcgtaca caagatttcc ccaacggtta
     4561 taaatctgca tgctaaatcc tacagattga atacctactc caacgggtaa aaaccgatca
     4621 ttggaaccat cgttattggg agtgaaagca tttgggaaat agactgcaaa atcttctgtt
     4681 acagtaatag ttttttgtaa ctgaatcgag catccaaatt cattggtagc aataagtgaa
     4741 actggatatt ccccaatttc ggtaaactga tacgaaggag aaatatcgtt gcttgtttgc
     4801 tcatccatcc gtggatcgaa atcccagata taactcaatg caccagaagt agtgttttcg
     4861 aaatgcacca aagcttctaa caagggaacg gtgtattgct ctgcttcaaa accaatctca
     4921 ggtagtggat gaaccgttaa ataggaatcg gcagtccatt gatttaaaca accatgttca
     4981 tctgtcactt gaagtgtgat tccaacattt gcagcatcgt cgtaacaaaa actaaccgat
     5041 tgtccggtat gaatcatatt gtgagaatcg ctccaaacgt aacttcctcc gggaacagct
     5101 gcagcactga attctgcaca cgtaccagca cagattgaag tttgagcact taggacttca
     5161 acacgtggta attcatacac caacaattga gttttgagtg tgtcggtaca gcctttagaa
     5221 tcctgaacaa ttacttgtac ttcatgtgtt tcaagtgcat tccaaacaat ctgatcttgt
     5281 gtttctcccc cactccaatt aaatgaaaaa ggtaatgttc cacctgtcgc agaagctgtt
     5341 agtagcgatg aatcaccttt acataaagct cgttcaccac taaattgaac gattaattct
     5401 tgtggctgca aaacttctcc ttgaagagaa attccacatc cattcgcatc agtaattaaa
     5461 acagaataga ttccagcggc tagattgtcg gcattgggtg attgacttcc gttactccaa
     5521 gcatattcat aaggcagaac gcctccatta gcctgagaaa gtagcatgcc agtcgcgctt
     5581 ccaaaacatg ttgcggcttg agcgtgcatt gaaccaatga gtaattcagg ttctgaaata
     5641 gttattgaat caacaatttg acaagcatgt gcatcggtaa tttgcacctg ataggctcca
     5701 gcagaaactt gatttaaatc tgcttgacct gcatacatgg aagtgctgtt tgaccataaa
     5761 aaagaataag gttgtgttcc tccattgatg attaaatgtg cagctccatc cgttcctcca
     5821 aaacaacgaa cattttctat agtatgtgaa ctaatcaatg catctggttg tgaaagattg
     5881 gtcaataatg tgtctttgca attcaattca tcactaacaa ctagcgtgta attaccagca
     5941 gctagtccgt taatacttgc acccaacaaa ttagaatttg accaattcaa acttaataaa
     6001 ccagagcctc cttgagccga aatttcaatc gccccatcag caccaccaaa acacgaaata
     6061 ttttgtgctt gaatcaatga aacgaccggc gctccgggat cttggaatga tttagttaat
     6121 gcagattgac atccatgcgc atcggtaact tgtaaggtat atgaaccact tgtgagacct
     6181 gtgatttgtg cttgatttcc tcctgtgctc cacgaatatt ggtaaggtga tgttccaccg
     6241 aaagcagaac cattcatact cccatttgct aatccacata cagatgaaat agtttcaatt
     6301 cctgcttgta aaacatctgg ttgattgatg gtaaaatcaa
//
LOCUS       DAOOQW010000130         6305 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_15064_length_6305_cov_5.378720, whole genome shotgun sequence.
ACCESSION   DAOOQW010000130 DAOOQW010000000
VERSION     DAOOQW010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 6305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6305
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15064_length_6305_cov_5.378720"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..460)
                     /locus_tag="PK281_07915"
     CDS             complement(<1..460)
                     /locus_tag="PK281_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49311.1"
                     /translation="MKRNILFFFLLGTGFIQAQSTAKKVLWIGNSYTYVNDLPNLFRL
                     LALSGGDSIIVDSSAPGGYSFADHTTNTTTLQKLAMNNNDFIVLQAQSQEPSFPPFQV
                     NTETFPYAAQLDSLAHLGSPCSQTVFYMTWGRKYGDAQNCPNYPPLCTFQG"
     gene            complement(457..1698)
                     /locus_tag="PK281_07920"
     CDS             complement(457..1698)
                     /locus_tag="PK281_07920"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOY49312.1"
                     /translation="MKKLQLILLFVISVFSSLTAQVSQYSSGLQPMKDGDNEMQVRNF
                     QKAYDNYTLAIVADPSLADAYYKRSIVLTKMARYSEALADQNKALQLNPNINQLYDVR
                     GQLRMLVEDFPGAVEDYTKAIENDPSEPELYNKLAHAHMASGDFDEALLDYSRMIKLD
                     PKDTLAYICRALAYLNLAQFDSAQIDINKAIQLNAQNSLAVEVQGLIYLEKKELPSSI
                     KAFDQAIKINPQFALAYYNRGIAWKMLGDAAKAEADFTRAIELDQNLSTAFFNRALAR
                     KKLGNEDEALEDYNKAIALDKDYAEAIFFRGLTKKLLGDYEGALEDFNKSERLNPNDP
                     EIYNNRANIRMLFGDYTSAIADYDKAIKLRENYAIAYYNRAVARIMMTKRGLACEDFN
                     KSLIFGYEPARQAVSDFCTTR"
     gene            complement(1720..2883)
                     /locus_tag="PK281_07925"
     CDS             complement(1720..2883)
                     /locus_tag="PK281_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOY49313.1"
                     /translation="MAKILIVDDERAIRSTLKEILEYENHQVDEAVDGAEGWEKIRSG
                     NYDILLSDIKMPRMDGTELLEKVLEQGIDCPVIMISGHGTIETAVECIKKGAYDFIAK
                     PLDLNRLMITLRNALDKSNLIQETKILKKKVSKTIDMVGESPAIRKVKDMIERVAPTD
                     ARVLVTGPNGTGKELVARWIHAKSNRSEAPLIEVNCAAIPSELIESELFGHEKGAFTS
                     AVKQRKGQFELAHGGTLFLDEIGDMSLSAQAKVLRALQENKITRVGGEKEIAVDVRII
                     AATNKNLQEEISRGNFREDLYHRLSVIIIQVPSLNERPEDIPLLAEYFLQLICTEYGI
                     PIKKINPDAVAEMQALQWTGNIREFRNVIERLIILCEKDITVQDVHAFARPAR"
     gene            complement(2876..3826)
                     /locus_tag="PK281_07930"
     CDS             complement(2876..3826)
                     /locus_tag="PK281_07930"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49314.1"
                     /translation="MKLAIIDIGTNTFKLMIARVQEDGQFIVIDKEKIPVKLGEGGIN
                     NHVIAHNPYLRGLKAMKTHKARIDRFEVDKTLAFATSAIRSAANGKEFVKKVKAETGI
                     DIEIISGDREAELIYYGVRQALDLGTEKVLIMDIGGGSTEFIIADRDRIYWKHSFDLG
                     AARLLEVINPSEPITKAEIKRLKAYLKDQMLLLWGACELYPVKTLVGSSGSFDSLAEM
                     IYYRFHTEENPLVKTEYNFDLDHFEAIYKILLNSTIEKRFKMKGLAAMRVEMIVVAVI
                     MIKYVMKKIKLNTMRLSTYSLKEGILFDHLRATKENENHG"
     gene            complement(3915..5249)
                     /locus_tag="PK281_07935"
     CDS             complement(3915..5249)
                     /locus_tag="PK281_07935"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOY49315.1"
                     /translation="MGKIGLIIQREYLTRVRKRSFILLTILGPLLMAGVAIVPIWLAN
                     ANETIRTVAVLDETGVVDYQLKSNENIQFQYTHGKLADAKKAMENSSTYALVYIPGEE
                     GDNLMTTQNNIKLYSKEQVSLNVKIYIENQIEKQIERAMLKSSGVDKDLVSSIEKTVK
                     VSIKNISLSEKGKSDTSNNVEISTILGGISGFLIYFFIFFFGAQVMRGVIEEKNNRIV
                     ELIISSVKPFQLMMGKIIGIAMVGLTQFLLWVILTASIYSVFMNTVVKEKFSAEKIEM
                     LKAKSPDGTAVEDIDQMADMNKMVDGINSVNWALIIPCFIFYFLGGYLLYGAMFAAVG
                     GAVDTDSDTQQFILPITIPLIFSFIMAQTVLNDPTGVMARWLSIIPLTSPIIMMVRLP
                     FQVPVWELILSMVMMIVGFVFTTWLAGKIYRTGILMYGKRVTWREMARWLRY"
     gene            complement(5253..6176)
                     /locus_tag="PK281_07940"
     CDS             complement(5253..6176)
                     /locus_tag="PK281_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007094286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOY49316.1"
                     /translation="MTTPLFEARNISKNYGNYRALDDVSISVEQGSVFGLLGPNGAGK
                     TSLIRIINQITIPDAGELLFKGKPMVLSDVQRIGYLPEERGLYKKMQVGEQCLYLARL
                     RGLDKQEAKRRLRVWFEKFEMQSWWNKKIEELSKGMAQKVQFVVTVLHEPELLIFDEP
                     FSGFDPVNASIIRNEILELRKKGATIIFSTHRMETVEELCDSIALINKSKKILDGKVR
                     DIQNEFRTNTFEVEFRGNMLAFTTAMWAGFELLEKHTEGNFCKVRLRMLGNNNVNSLF
                     QAVMPVCEIVSFREVIPTMDEIFIRNVQSSI"
BASE COUNT         1909 a         1393 c         1068 g         1935 t
ORIGIN      
        1 tcccttgaaa tgtgcataaa ggaggataat tgggacaatt ctgtgcatca ccatattttc
       61 ttccccaagt catgtaaaaa accgtttgcg aacaaggcga tccgagatgt gccaaagaat
      121 ctaattgagc agcatatgga aatgtctctg tattaacttg aaagggagga aaagaaggct
      181 cttgactttg agcttgaagc acaatgaaat cattattatt cattgctagc ttttgcagtg
      241 ttgtagtatt agttgtgtga tcagcaaacg agtaacctcc tggcgcgcta gaatcaacta
      301 taatggaatc gcctccacta agagctaata gccggaaaag attaggcaaa tcattcacat
      361 aggtataact gttcccaatc caaagcactt tcttggctgt tgattgtgcc tgaataaatc
      421 cagttcctag taagaaaaag aagagtatgt ttcttttcat cgtgtagtac agaaatcaga
      481 aacagcttga cgtgctggtt catatccaaa tatcaagctt ttattaaaat cttcacaggc
      541 taatccgcgc tttgtcatca ttatacgagc aactgcacga ttataataag caatagcata
      601 attttcacgg agcttaatag ccttgtcata atcagctata gcactggtgt aatcaccaaa
      661 tagcatccga atattagctc ggttattata tatttctgga tcattcggat tgagtctttc
      721 acttttatta aagtcttcca aagcaccctc gtaatcacct aatagctttt ttgttaagcc
      781 tctgaaaaag atagcttctg cgtaatcttt atctagtgca atagctttat tgtaatcttc
      841 taatgcttca tcttcattac ccaatttttt tcgtgctaat gctcgattga agaaagcagt
      901 agataagttc tgatccaatt cgatagcacg agtaaaatct gcttccgcct ttgcagcatc
      961 acccaacatc ttccaagcta ttcctctatt ataataggca agggcaaact gtggatttat
     1021 ttttattgcc tgatcaaatg ctttgataga agatggcagt tcttttttct ctaaataaat
     1081 caatccctga acttcaactg ctaatgaatt ttgagcatta agctgaatcg ctttattaat
     1141 atcaatctgg gctgaatcaa attgagctag attcaggtaa gccaaagccc gacagatata
     1201 tgcaagcgtg tctttgggat caagcttaat catgcgggaa taatccaata aggcttcatc
     1261 aaaatcgcca gaagccatat gtgcatgagc aagtttattg tacaactccg gttcggaagg
     1321 atcattctca atagcctttg tgtaatcctc aactgcacct ggaaaatcct ccaccaacat
     1381 gcgcaattgt ccacgaacat catacaattg attgatgtta ggattgagtt gaagagcttt
     1441 gttttggtcg gctagtgctt cagaataacg cgccatcttc gttagtacga tacttcgttt
     1501 gtaataagca tctgccaacg aaggatcagc aacaatagct aatgtatagt tatcgtaagc
     1561 cttttgaaaa tttcgcactt gcatttcatt gtcgccatct ttcattggct gcaaaccaga
     1621 actatactgg cttacctgtg cagtaagtga tgaaaaaaca ctaataacga acagcagaat
     1681 cagttggagt tttttcataa gcaaaattta gtttttcact taacgcgccg gtcgggcaaa
     1741 agcgtgtacg tcttgtacag taatatcctt ttcacaaaga ataataagtc gttcaattac
     1801 atttcgaaac tcacgaatat tccctgtcca ttgcagcgcc tgcatttcag caacagcatc
     1861 cggatttatt ttcttaatcg gaattccata ttctgtacag attaattgta gaaaatattc
     1921 ggctagaagt ggaatgtctt ccggtcgttc atttaaagaa ggaacttgaa tgataattac
     1981 actcaaccga tgatataaat cctctctaaa atttcctcga ctaatttcct cttgcagatt
     2041 cttattcgtt gctgcaataa tgcgaacatc aaccgctatc tccttttctc cccctactcg
     2101 ggtgatctta ttctcctgca atgcacgtaa aaccttggct tgagccgaga gactcatatc
     2161 accaatctca tctaagaaca atgttccacc atgagccaat tcaaactgac ctttacgttg
     2221 ttttactgca cttgtaaaag ctcctttctc atggccaaac aattcgcttt caatgagttc
     2281 ggatggaata gcggcacagt ttacttcaat taaaggagct tcactccggt tactttttgc
     2341 gtgaatccaa cgagcaacca attcttttcc agtaccattt ggacctgtaa ctaaaacgcg
     2401 agcatcggtt ggagcaacgc gttcaatcat gtctttcact ttacgaatgg caggagattc
     2461 acccaccata tcaatggttt tgctgacttt cttcttaaga attttggttt cttgaatgag
     2521 attcgattta tccaacgcat ttcgaagcgt aatcattaac cgattcaaat ccagcggctt
     2581 agcaataaaa tcgtaagctc cttttttgat gcactcaacg gctgtttcaa ttgttccgtg
     2641 cccggaaatc ataataaccg gacagtcgat accttgttcc aaaacctttt ccagcagctc
     2701 agttccatcc atacgaggca tcttgatatc acttaacaga atatcgtaat taccagaacg
     2761 gattttctcc catccttcag caccatcaac agcttcatcc acttgatgat tttcgtattc
     2821 gagaatttct ttcaacgtgc ttcgaattgc acgttcatcg tctacgataa gaattttagc
     2881 catgattttc gttttcttta gtggcacgta agtgatcgaa gagaattcct tctttaagtg
     2941 aataggtgga taagcgcata gtattgagct tgattttctt catcacatat ttaatcatta
     3001 ttactgcaac aacaatcatt tctacgcgca ttgctgcaag tcctttcatc ttgaaacgct
     3061 tctcaattgt tgagttgagt agaatcttat agattgcttc aaaatggtcg aggtcgaaat
     3121 tgtattctgt ttttactagc ggattttcct cggtatggaa gcgatagtaa atcatctccg
     3181 ccaatgaatc aaaactacca gaagagccaa caagtgtttt caccggatac aattcacaag
     3241 caccccaaag caacaacatt tgatctttta gatatgcttt cagtcgtttg atttctgctt
     3301 tggtaatggg ttctgatgga ttgatgactt ccaataaacg agcagcaccc aaatcaaaac
     3361 tgtgtttcca atagatgcgg tcacggtcgg caatgatgaa ttcggtactt ccaccaccaa
     3421 tatccatgat cagcactttc tccgttccta aatcgagggc ttgtcgaacg ccataataaa
     3481 tcaactcagc ttcacgatcg cccgaaataa tctcaatatc tattccggtt tctgctttaa
     3541 ctttcttcac aaattccttc ccgttggcag cacttcggat agcagatgtg gcaaatgcca
     3601 aagtcttatc cacttcgaat cgatcaatcc gagccttatg agttttcatg gcttttaaac
     3661 cacgcaaata aggattatga gcaattacat ggttattaat gcctccttct cccaacttaa
     3721 cgggaatctt ctctttatca attacgataa actgaccatc ctcttgaaca cgagcgatca
     3781 tgagtttaaa cgtattggta ccgatgtcaa taatagcgag cttcacacag ggaaaattga
     3841 agtttcaatg ttaagaaatc cctatgctga ttcctgcata gaatgttaac aggttattaa
     3901 cctaatctgt aacattaata acgtagccaa cgcgccattt cacgccatgt tacacgcttc
     3961 ccatacatca aaattcctgt ccgataaatt tttcctgcaa gccaagtggt aaacacaaaa
     4021 cccacaatca tcattaccat cgataaaata agttcccaaa caggcacctg aaatggtaaa
     4081 cgaaccatca taataatggg tgatgtaagt ggaataatgg ataaccatcg agccataact
     4141 ccagttggat catttagaac cgtttgagcc atgatgaatg aaaaaatcag cggaatggta
     4201 ataggtaaga tgaattgctg ggtatcagaa tctgtatcaa cagctcctcc aacggccgca
     4261 aacatggctc cgtaaagtaa atatccccct aagaaataaa agataaagca agggatgatc
     4321 aaagcccaat tcacactgtt aattccatct accattttat tcatatcggc catctggtca
     4381 atatcttcta cggcagttcc atcaggactt ttcgctttta acatctcgat tttctccgca
     4441 ctaaatttct ctttcacaac agtattcatg aaaacagaat agatagatgc agtaagaatt
     4501 acccaaagta aaaattgagt taatccgacc atagcaatac caatgatttt acccatcatc
     4561 aactgaaaag gcttaacact ggaaatgatc aattcaacaa ttcggttgtt tttctcttca
     4621 atcacacctc gcattacctg cgcaccaaaa aagaaaatga agaaataaat cagaaaacca
     4681 gaaataccac cgagaatcgt acttatctcc acattattac ttgtgtcgct tttccctttc
     4741 tcgcttaagg agatgttctt aatcgaaact ttaactgtct tctcgatact cgaaactaga
     4801 tctttatcaa caccactcga tttaagcatg gcacgttcaa tctgcttctc aatctgattt
     4861 tcaatgtaaa ttttcacatt caaactaacc tgttctttcg aatagagctt gatgttattt
     4921 tgagtcgtca tgagattatc accttcttca cctggaatat ataccaaagc gtaagtggat
     4981 gaattctcca tggctttctt agcgtcagct agctttccat gtgtgtattg aaactggatg
     5041 ttttcgttag atttaagttg ataatctacc acacctgttt catctaaaac ggcaactgtt
     5101 ctaattgttt cattcgcatt ggcaagccaa ataggaacga tagccacacc agccatcaac
     5161 aatggtccaa gtatggttaa aagaataaac gaacgtttac gaactcgggt tagatactcc
     5221 ctttgaatga tcaatcctat ctttcccatg atttatattg atgattgaac gttgcgaata
     5281 aaaatctcat ccatggtagg aattacctcc cggaaactta caatctcaca aacgggcatt
     5341 actgcctgaa ataaactatt tacgttgtta ttgcccaaca tgcgtaaacg aaccttacaa
     5401 aaattgcctt ctgtatgctt ttcaagtaat tcaaagccag cccacatagc agtcgtgaag
     5461 gctaacatat tacctcgaaa ctcgacttca aacgtatttg tccgaaattc attttgaata
     5521 tcacgcactt taccgtccag aatcttcttg ctcttattga ttaatgcaat actatcgcac
     5581 aattcttcca ctgtttccat tcggtgagta gagaaaatta tggttgctcc ttttttacga
     5641 agctctaaaa tctcattccg aatgatcgaa gcgttaacgg gatcaaatcc tgaaaatggt
     5701 tcatcaaaga tcaaaagctc cggttcatgt aatacagtta ctacaaactg aactttctgt
     5761 gccatccctt tcgataactc ctcaatcttc ttattccacc acgattgcat ctcgaatttc
     5821 tcaaaccaaa cgcgaagtcg acgtttagct tcttgcttat ccaatccacg cagtcgagct
     5881 aaatacaggc attgttctcc aacctgcatc ttcttatata atccgcgctc ttcgggcaaa
     5941 taaccaattc gttgtacatc cgacaaaacc attggcttgc ctttaaacaa aagctcacca
     6001 gcatcaggaa tagtgatttg gttgatgatt cggattaatg aggtttttcc agctccgttt
     6061 ggtcccaaca aaccaaatac agaaccttgt tctaccgaaa tactcacatc gtccaaggct
     6121 cggtaattac cgtaattctt ggagatattt cgggcttcaa ataggggtgt cgtcatattc
     6181 aaattaaaac ggccacaatg tggccgtcaa atataccaaa gtactatagc ttaatggaag
     6241 tattccttca tccgttcaaa aaaggatttc tctttcttac ccggctgtgg aataaaatta
     6301 ttggc
//
LOCUS       DAOOQW010000131         6303 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_15072_length_6303_cov_5.015685, whole genome shotgun sequence.
ACCESSION   DAOOQW010000131 DAOOQW010000000
VERSION     DAOOQW010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 6303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6303
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15072_length_6303_cov_5.015685"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..952)
                     /locus_tag="PK281_07945"
     CDS             complement(<1..952)
                     /locus_tag="PK281_07945"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004919840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOY49317.1"
                     /translation="MEKIQMVDLKAQYRRIKADVDQAIQQVIDTTTFINGPAVHDFQK
                     DLEAYLGVKHVIPCANGTDALQIAMMALGFKPGDEVITASFTYVATAEVIALLGLKPV
                     LVDVDPKTFNIDPDALERAITSKTVAIVPVHLFGQCADMDRIMEIANKHKIHVIEDTA
                     QAIGAEYTSKDGSVRKAGTIGTIGATSFFPSKNLGCYGDGGALFTNDDKLAERLRMIA
                     NHGQRVRYYHDDIGVNSRLDSIQAAILRVKLRELDNYAAARRKLADAYDAAFTPTGKL
                     ITPIRATNSTHVFHQYTMQVNGVDRDKMKEFLESKGVPAMI"
     gene            1042..1800
                     /locus_tag="PK281_07950"
     CDS             1042..1800
                     /locus_tag="PK281_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49318.1"
                     /translation="MNRFYLITFCILITASSLFSQVNVKDSLLAFSTVNVSFAWQIPS
                     GDLAKRFGDFGSVGMNYLRKQRSGLIWGVNGNFLFGGDVKQKDMLQNIITGDGFVIGA
                     DGTLYEAKYLMRGIQFDARIGKVFPIGGPNKNCGIYTTIGAGFLQHKIRIDTERNANV
                     PPLTKEYVKGYDRLSNGWSINPALGYLFLSNNRSVNFFVQVEYLYSQTENRRSFNYDT
                     GLHDATIRKDGSLGFRFGWVLPIYKAPATDFFYY"
     gene            1806..3023
                     /locus_tag="PK281_07955"
     CDS             1806..3023
                     /locus_tag="PK281_07955"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HOY49319.1"
                     /translation="MFFLYQLSIRLYILAIQLASFRSKKAAQWIQGRKNQHWENIRKP
                     DEKWVWFHCASLGEFEQGRPVIEALKKELPHIKIVLSFFSPSGFEVRKNYPVADSIIY
                     LPADLPANVNRFLQAFQPELAVIVKYEFWFGYLRALKEKEIPTYLISARFRKNQHFFQ
                     PWGFWFRKQLACFTEIHVQDKESEVLLHHVGYTTTQVSGDSRYDRVAENASYPAPRIE
                     IENWIAGRKVLIAGSTWSEDDEIVFPWNSNEWAIIVAPHEISEARIEQVEKSCGKSSV
                     RYTQLSSGNKQSEVLILDNMGMLLSVYALGDVAYVGGAFGKGLHNILEPAACGLPVIF
                     GPKYSKFLEAEELIQAGGGFSISNRESFLKTFSALSNPDTLKAKSALCASFVQSKTGA
                     TQKIIRSLVSQLN"
     gene            3065..3775
                     /locus_tag="PK281_07960"
     CDS             3065..3775
                     /locus_tag="PK281_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49320.1"
                     /translation="MTSLSLQAQDKIKTVGFQIKPLFFSSFFGTGPQSLSDSSYSYTL
                     KPSSGYAAGMVIRQGYTPTLSIEFGINYVVRNVSITASHASQTASNSLRIIGYEIPFS
                     QLIFIRLGEHTFMNAGAGICLNIFPSDVIKRDSTFAAYGGRKHSFVPSLLANIGVEYR
                     TPKSGYFYFGASLNRPFGPIYNVAIDYIQNGIVQNSTLGSLNGSYLSADFKYFFPEDP
                     EKKKVRKAKRKKEEPVVK"
     gene            4029..4113
                     /locus_tag="PK281_07965"
     tRNA            4029..4113
                     /locus_tag="PK281_07965"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4063..4065,aa:Leu,seq:taa)
     gene            4264..>6303
                     /locus_tag="PK281_07970"
     CDS             4264..>6303
                     /locus_tag="PK281_07970"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HOY49321.1"
                     /translation="MKRLFFLTNLLFLLVQPAFAQWRSSNPYNGAINDLFFTNRLTGF
                     ACGQAAGVGGCSGTTSIIRTVDGGETWIRMNTGSTAAMTRLHFVDSFTGWAVGASSSI
                     IKTTDGGQTWTIPSSGVGAGLNDIHFPNANVGYVVGANGLVRKSSNGGSSWTTQTSGV
                     TVTLNSVWFADANTGYFVGNSGTIRKTTNGGTTWTSVYTGTDFFREVWFADANNGYVL
                     SNNKILKTTNGGSTWNSFDAPSGMVWLRMNFSSMNSGVIVSDSDVLLKTNDGGQTWNQ
                     QTTDFNETMACAFFLDENYGFTGSYSLGRIVRTLDGCQNWTNVNSGMGERVEGIDFRT
                     PQKGVFVSSAGGIFTTRNGALNVRKMHSNTSHALLAVNWMNDSTIIACGDSGTILRST
                     NSGWSWTSIPTGTIEPLLDMVAVDSLVAYSSGGNGMVIKTQDAGLSWTTVAVDSLLPL
                     RGIHFLNRDTGMTVSDYAVFKTINGGLSWELKNDSIQSTSDFNDVWMTSPYVAYAAGG
                     FGRMYKTVDGGEIWRRVFPSDNTNAEIEEMQFFTDSIGYFARLNSQYFTLNGGTVVGS
                     QSTYCLANNGGVDAIEMPNQQYGYCAGGISNVLHTLKPMEMLRTYLQDSIYCSGSRIF
                     VGYNAAGLLYNSNVMNVELSNSSGDFSSPTVIGSYTVQLPETDPSGIITCTLPTGI"
BASE COUNT         1869 a         1129 c         1301 g         2004 t
ORIGIN      
        1 agatcatcgc tggaacacct ttcgattcga gaaattcctt cattttatct cgatctacac
       61 cattcacttg catggtatat tgatgaaata catgagttga atttgtagct ctaattggag
      121 tgattaattt tcctgttgga gtaaaagcag catcgtatgc gtcagcaagt ttgcgacgag
      181 cagctgcata attatccagt tcgcgaagtt taacacgaag aatagctgcc tgtattgaat
      241 caagacgaga atttactcca atatcatcat gataataacg aacccgttga ccatgattcg
      301 caatcatacg aaggcgttcg gctagtttat catcgttggt aaataaagca ccaccatcac
      361 cataacatcc aagattctta gatgggaaaa atgatgtagc tccaatagta ccaatggttc
      421 cagcttttct aactgaacca tctttggaag tatattctgc tccaatagct tgagcagtat
      481 cttctatcac atggatttta tgcttattag caatctccat gatccgatcc atatcggcac
      541 attgtccaaa caaatgaaca ggaacgatag caacagtttt acttgtaata gctcgctcta
      601 aagcatctgg atcgatgtta aatgttttag gatctacatc aaccaataca ggctttaatc
      661 caagaagtgc tataacttca gcagtagcca cataagtgaa actagctgta attacttcat
      721 cacccggttt aaaccctaat gccatcatgg caatctgaag cgcatcggtt ccatttgcac
      781 atggaattac atgcttgact cctaaatatg cctctaaatc tttttgaaaa tcgtgtacgg
      841 caggaccatt aataaatgtt gtcgtatcaa ttacttgctg aatggcctga tcaacatcgg
      901 ctttaatgcg ccgatactga gctttcaggt ctaccatctg tatcttttcc aagctaaaaa
      961 aatttgtgcg aagatacata ttttaagaga tcagctttcg ttttacctca acgttcacaa
     1021 tctcgatatc tttgtccctg catgaatcgt ttctacctga ttactttctg tattcttatc
     1081 actgcttcca gcctgttttc acaggtaaat gtgaaagatt cactcctcgc tttttcaact
     1141 gttaatgtga gctttgcctg gcaaattcca tccggagatt tagctaaacg gtttggagat
     1201 tttggatctg ttgggatgaa ttatttaagg aagcaacgtt ctggattaat atggggcgta
     1261 aatggtaatt tcctatttgg tggagatgtg aagcaaaaag atatgcttca gaatattata
     1321 actggagatg gttttgtgat tggagcggat ggtaccttgt atgaagccaa atatcttatg
     1381 cgaggaattc aatttgatgc tcgaattggt aaggtattcc cgattggtgg gcccaataag
     1441 aattgcggga tttataccac tataggtgcc ggattccttc aacataaaat tcgaatcgac
     1501 acagaacgaa atgcgaatgt tcctccactt actaaagagt atgtaaaagg atatgatcgt
     1561 ttaagtaatg gttggtcgat aaatccggca cttggatatt tgttcctaag taataatcgc
     1621 tcggtcaatt tttttgttca agtagagtat ttatatagtc aaacagaaaa tcgtcgatcg
     1681 tttaattatg atactggatt gcacgatgcg acgattcgta aagatggttc tttaggattt
     1741 cgttttggtt gggttttgcc tatttacaaa gctccagcaa cagacttctt ttactattaa
     1801 ttcggatgtt tttcctttat cagcttagta tccggcttta tattctagcc atacaattag
     1861 cctcttttag gtcgaaaaaa gcagcgcaat ggatacaagg aagaaagaat cagcattggg
     1921 aaaacattcg caaaccggat gaaaaatggg tttggtttca ttgtgcttcc ttgggtgaat
     1981 ttgaacaggg aagaccagtc attgaagcac taaaaaaaga attaccacat ataaagattg
     2041 ttctaagctt cttttctcct tcgggttttg aggtgcgtaa aaattatcca gttgccgata
     2101 gtattattta cttaccagct gatttaccag ccaatgtgaa tcgattccta caagcttttc
     2161 aacccgaatt agccgtgatt gtgaaatatg aattctggtt tgggtattta agggctttga
     2221 aagaaaaaga aattccaacc tatctcatat cagcacgttt tagaaaaaac cagcatttct
     2281 ttcagccttg gggattttgg ttccgaaaac aattagcgtg ttttacagaa attcatgtgc
     2341 aggataaaga atcagaagta ttacttcatc atgttggcta taccactacc caagttagtg
     2401 gcgattcgcg ttatgatcga gtggcagaaa atgctagtta tccagcacca cgcatagaaa
     2461 ttgaaaactg gattgcagga agaaaagttt taatcgcagg tagtacttgg tccgaggatg
     2521 atgaaatagt gttcccatgg aattcgaatg aatgggctat tattgttgca cctcatgaaa
     2581 tttcagaagc tcgaatagaa caggttgaaa aatcatgtgg taagtcgagt gtgagataca
     2641 cccagcttag ttcgggtaat aaacaatctg aagtattgat tttggataat atgggaatgc
     2701 tactttcggt ttatgcattg ggtgatgtag cttatgtggg aggagcattt ggaaaaggat
     2761 tgcataatat tcttgaacca gcagcatgtg gacttccagt aatatttgga cctaaataca
     2821 gcaagttctt agaggctgaa gagctaattc aagcaggtgg tggtttttct atatcgaata
     2881 gagaatcatt tctgaaaact ttttctgcgc tttctaatcc agatacttta aaagccaaat
     2941 ctgcactttg tgctagtttt gttcaatcaa aaacgggtgc aactcaaaag atcattcgct
     3001 cattagtatc ccagttgaat taaacatgag aaaattgagt cgcatatttc tagtgtttat
     3061 ctgcatgaca agtctatcct tgcaagcgca ggataagatc aagacagttg gatttcaaat
     3121 taagccatta tttttctcgt cattctttgg tactggtcca caatcattat ctgacagttc
     3181 ctattcctat acattaaaac caagttctgg atatgctgca ggcatggtca ttcgtcaagg
     3241 ctatactcct actctatcca tagaatttgg aattaattat gtagttcgaa atgtatctat
     3301 tacagcaagt catgcatctc aaactgcaag taattcactt cgaataattg gatacgaaat
     3361 tccattcagc caattgattt tcattcgttt aggtgaacat acatttatga atgcaggtgc
     3421 agggatttgt ttgaatattt ttccttcgga tgtcattaaa agggattcga cttttgccgc
     3481 ttacggtgga aggaaacata gttttgtgcc aagtttatta gccaatattg gagttgaata
     3541 ccgaacacct aaaagtggat atttctattt tggcgcatca ttgaatcgtc cattcgggcc
     3601 catatataat gtagctattg attatattca aaatgggatt gtacagaatt caactttagg
     3661 aagtttaaat ggctcttatt tgtcggccga tttcaaatac ttttttccgg aggatcctga
     3721 aaagaaaaag gtgagaaaag ccaaacgtaa aaaagaagaa cctgtagtta aataatattt
     3781 caagcccgat attattctgt ttcatatagt tcccgattca gattcttgaa tttggctact
     3841 caaattaaat gtttgtcgat atgttcaata ccaatttcat gtgcttgata tttcattcaa
     3901 attctccatt taattcctgc ttgcaattat taaacaatta agataatcaa gcgaatacac
     3961 gtttttattg agaaagaagc gatttctctt taacagaata aaaatattta tacttttgcc
     4021 cccgtgttgc ccggatggtg gaattggtag acacgcagga cttaaaatcc tgtgatcatt
     4081 gcgatcgtgc gggttcgatt cccgctccgg gtacttttca aaaagtgatt tgtatatcac
     4141 tactctttat ctaaaagccg ctatatgcgg cttttttcat tttctcactt ttacgatgaa
     4201 cattctttga caaatagctt aatgtgactc gcactgctta aatttgtcaa cccatacaaa
     4261 accatgaaac gacttttctt cttaactaat ttgttgttcc ttttagttca accagctttt
     4321 gctcaatggc gttcttctaa tccttacaat ggagcaatta atgatttgtt cttcaccaat
     4381 cgattaactg gctttgcttg tggacaagcg gcaggtgttg gaggatgctc aggtacaact
     4441 tcaatcattc gcacagtaga tggaggtgaa acttggatac gaatgaatac cggctcaaca
     4501 gctgctatga cacgccttca tttcgtggat tcatttactg gttgggctgt tggagcatca
     4561 agctccatta ttaaaactac cgatggtggt caaacatgga caattccatc ttcaggagtt
     4621 ggggcaggat taaacgacat acatttccct aatgctaatg tgggttacgt agttggagct
     4681 aatggcttag ttcgaaaaag ttcaaatgga ggatcaagct ggactaccca aacttctggg
     4741 gtaactgtta cattaaattc ggtttggttc gcagatgcaa atacaggcta tttcgtagga
     4801 aattctggaa cgataagaaa aacaacgaat ggtggaacca catggacaag tgtgtatact
     4861 ggtaccgatt ttttccgtga agtgtggttt gcagatgcta ataatggata tgtgctgagt
     4921 aacaacaaaa tcctcaagac taccaatggt ggaagtactt ggaacagctt tgatgcacca
     4981 tctggaatgg tttggcttcg tatgaatttc agtagtatga attcgggcgt tatcgtaagt
     5041 gattcggatg ttttattaaa aacaaacgat ggcggtcaaa cctggaatca acagactacc
     5101 gattttaacg aaactatggc ttgtgcattt ttcttggatg aaaactatgg ctttacagga
     5161 tcctattcat taggcagaat tgttcgtaca cttgatggtt gtcaaaactg gacgaatgta
     5221 aatagtggta tgggtgaacg tgtggaagga attgattttc gcactccaca aaaaggcgtt
     5281 ttcgtaagta gcgcaggagg aatttttacc acacgaaatg gtgccttgaa tgtgcgcaaa
     5341 atgcatagta atacttcaca tgctttatta gcggttaatt ggatgaacga cagtaccatt
     5401 atagcatgtg gtgatagtgg aacaatactt cgatcgacca attcgggttg gagctggact
     5461 tcaataccaa ccggtacaat cgaacctttg ctcgacatgg tggcagtaga ttctttagtt
     5521 gcttattctt cgggaggaaa tggaatggtt attaaaacac aagatgctgg attatcttgg
     5581 actaccgtcg cagttgattc tcttttgcct ttaagaggaa tccatttttt aaaccgagat
     5641 acaggaatga ccgtttcgga ctacgcagtt ttcaaaacta ttaatggagg attaagctgg
     5701 gaactaaaga atgatagtat tcaaagtact tctgacttta acgatgtgtg gatgacgagt
     5761 ccgtatgttg cttatgctgc aggtggattc ggtcgcatgt ataaaacggt tgatggtggt
     5821 gaaatatgga gacgagtttt cccttcggat aataccaatg ccgaaattga agaaatgcag
     5881 ttctttaccg attctattgg atatttcgct cgtttgaata gtcagtattt tacgcttaat
     5941 ggcggaacgg ttgtgggttc acaatcgacc tattgtttag caaataacgg tggtgtagat
     6001 gcgatagaaa tgcctaacca acaatatgga tattgtgcag gcggaattag taatgtgctc
     6061 catactctaa agccgatgga aatgcttcgt acttatttgc aggattcgat ttactgttca
     6121 ggaagtagga ttttcgtggg atacaatgca gcaggattgc tatacaatag taatgtgatg
     6181 aatgtagaat taagtaacag ctctggagat tttagctctc caactgtaat tggttcttat
     6241 accgttcaat tgcccgaaac cgacccttct ggaatcatca cctgtacttt acctacaggt
     6301 att
//
LOCUS       DAOOQW010000132         6274 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_15151_length_6274_cov_6.002090, whole genome shotgun sequence.
ACCESSION   DAOOQW010000132 DAOOQW010000000
VERSION     DAOOQW010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 6274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6274
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15151_length_6274_cov_6.002090"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="PK281_07975"
     CDS             complement(<1..530)
                     /locus_tag="PK281_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49322.1"
                     /translation="MPITKSPISFRIFLLVGIYFLFLGKSGLAQKFSFLNVPKEVFTH
                     KGFGEKDSADVVILFDSASVRFSSIGENFTIITTRYQRMKILTQDGVDNGTVVIKRFS
                     NADFLKKQPIFHAEGATFNLSKDQSVRMTKLDSTEIFKNELTLKLAFSNVKPGSVIDY
                     SYSIEEPVHINLCDWE"
     gene            complement(706..2043)
                     /locus_tag="PK281_07980"
     CDS             complement(706..2043)
                     /locus_tag="PK281_07980"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49323.1"
                     /translation="MPTNFPLGFQEHIQDLPGINTTSFLLAHEEKAPVSIRLNPEKLN
                     SAFENETKVAWCEHGRYLPSRPDFTLDPLFHTGAYYVQEASSMSIWSALTDLVPEKSN
                     LRVLDLCAAPGGKSTLIASWLAGEGMLVANEVIRSRAGILHENLNRWGSANTIVSNND
                     PRQFGKLEGYFDVIVIDAPCSGSGMFRKDPAAMSHWSEDAVTHCAARQERILTDVWPA
                     LKEGGLLLYSTCSYSEAEDEQISSFIKEELAAEICAIPSLENHPEIVKTSMGYRFYPG
                     QILGEGFYLSAIRKTAETENPYHKPAFRPQVIPNQIAHWMSNPDAFVYSETHLGSSVL
                     PASLHESIPPLLKHLHILKCGILAGEMKGKDFLPSHEMALSTLLRNDIPQLELEREEA
                     LRYLKKEALPGRSDIKGWHRMNFQGLGLGWGNVLPNRINNALPKSWRILKELE"
     gene            complement(2103..2447)
                     /gene="rplT"
                     /locus_tag="PK281_07985"
     CDS             complement(2103..2447)
                     /gene="rplT"
                     /locus_tag="PK281_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006989809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOY49324.1"
                     /translation="MPRSVNHVASRARRKKILNQTKGYWGARKNVFTIAKNILEKGLT
                     YAYRDRKTKKRNFRGLWIQRINAGVRQHGMSYSEFMGKIHAKNIQINRKVLADLAMNN
                     PEAFKAICDAVK"
     gene            complement(2529..2729)
                     /gene="rpmI"
                     /locus_tag="PK281_07990"
     CDS             complement(2529..2729)
                     /gene="rpmI"
                     /locus_tag="PK281_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HOY49325.1"
                     /translation="MPKMKTNSSAKKRFKLTASGKIKRKHAFMRHILTKKSKDQKRRL
                     GHSGLVCKADEGLVKRMLTIGK"
     gene            complement(2754..3314)
                     /gene="infC"
                     /locus_tag="PK281_07995"
     CDS             complement(2754..3314)
                     /gene="infC"
                     /locus_tag="PK281_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOY49326.1"
                     /translation="MPPRSPRPFNRGPQKKEEAHRINLKIVGVPKVRLVGDGIEPAVY
                     SFEDALRVAREQELDLVEIVPNADPPVCRVIDYKKFLYEQKKKQKEMKAKATKIVVKE
                     VRFGPNTDEHDFNFKLNHARKFLQEGSKVKAFVFFKGRSILFKEKGEILLLKFAAELE
                     ELGKAESMPELEGKRMIMYISPKKKK"
     gene            complement(3348..5282)
                     /gene="thrS"
                     /locus_tag="PK281_08000"
     CDS             complement(3348..5282)
                     /gene="thrS"
                     /locus_tag="PK281_08000"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOY49327.1"
                     /translation="MINITLPDGSVRQYESGVTALDIAKSISEGLARNVLAATVNGEV
                     QDATRIIQTDSTVKLLTWNDDDGKKTMWHSSAHLMAEALEELYPGIKFGIGPPIENGF
                     YYDVDLGDRSISAEDLPAVEAKMKELAKKKSQYVRKDMSKADAIAYFTEKGDEYKLDL
                     LAGLEDGTITFYEQGNFTDLCRGPHIPDTGFIKAVKLMNVAGAYWKGDEKNKQLTRIY
                     GITFTKAADLDEYLVLLEEARKRDHRKLGKELELFMFSEKVGQGLPMWLPKGTALRER
                     LVDYLKRAQASTGYVQVITPHIAHKDLYVTSGHYEKYGADSFQPIKTPAEGEEFFLKP
                     MNCPHHCEIYKNKPHSYKDLPIRLAEFGTVYRYEQSGELHGLTRVRGFTQDDAHIFCR
                     PDQVKEEFLKVIDLVFMIFKALDFKDFTAQISLRDPENKTKYIGSDENWEKAENAIIE
                     AAAEKGLRTVVELGEAAFYGPKLDFMVKDAIGRRWQLGTIQVDYNLPERFQLEYMGAD
                     NAKHRPVMIHRAPFGSLERFVAVLIEHCAGKFPLWLTPEQAVILPISEKYNEYGKKVL
                     QLLKNYDIRAAIDDRDERAGKKIRDAEIMKTPFMLVVGEKEAETGSVSVRKHGEGDLG
                     TMSLEAFAALIEEEVNKQMN"
     gene            5381..5830
                     /locus_tag="PK281_08005"
     CDS             5381..5830
                     /locus_tag="PK281_08005"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOY49328.1"
                     /translation="MPFSRLFLIISLIFSSPTLINAQILREPIVEPTEPPDVDTKEQV
                     FTIVEEMPVFENGKDDINTFLAKNINYPLECKESSIQGTVFIKFVVQAAGGIRDIQVM
                     KSPKECNMLEKEAIRVVKLSGNYWKAGKQNGKKVDVYNILPIRFKLQ"
     gene            5865..>6274
                     /locus_tag="PK281_08010"
     CDS             5865..>6274
                     /locus_tag="PK281_08010"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOY49329.1"
                     /translation="MRFLISYLFLLVSFCVESQTVTPSKTDRIDERRTSDGESYPVQD
                     IIPTKSDIYSVVEEQAHYKYGDEALLKFISKNIIYPPSAVEIEIQGKVYVQFVVNTDS
                     TLSDITVVRGINGGEMLNKEAIRVVKLTSGDWVPA"
BASE COUNT         1756 a         1282 c         1275 g         1961 t
ORIGIN      
        1 aattcccaat cacataaatt gatatgaacg ggttcttcta tgctgtatga atagtcaatt
       61 acagatcctg gtttaacatt cgaaaaggcg agttttagtg taagttcatt tttaaatatt
      121 tcggtagaat ccaatttcgt cattcgtacc gattgatctt tactcaaatt aaatgtagct
      181 ccttcggcat ggaaaatagg ttgctttttg aggaaatcag catttgaaaa tcgttttatt
      241 accactgtcc cattgtctac accatcttgc gttaatattt tcatgcgttg atagcgcgtg
      301 gttatgatgg tgaaattttc accaatggaa ctaaaacgaa ccgatgctga atcgaataat
      361 atgactacat cggcactgtc tttttcgcca aatcctttat gggtaaaaac ttctttagga
      421 acatttaaaa aagagaactt ttgtgctaga ccactttttc ccagaaataa gaaatatatt
      481 ccaactaata agaatattcg gaatgagatt ggagatttag ttataggcat aaatgatttc
      541 aatatacaag cgcaacaatt tagtaactaa aaacaaattt gttgtcattg catattgatt
      601 tattctacac ggttctcgca caagggattg agccagagga acgaagcgac gaggcgaaag
      661 cccggttttt tggcgacgaa ggaggcaaaa gaatgtgccc taaccttatt ctaattcttt
      721 caatattcgc cagctcttag gtaaggcatt attaatccga ttaggcaaca catttcccca
      781 gccaagccct aatccttgaa aattcatgcg atgccagcct tttatatcgc ttcttccagg
      841 aagggcttct ttttttaagt aacgcaacgc ttcctcacgt tcgagttcta actgaggaat
      901 atcgtttcgt aacagcgtgc tcaaagccat ttcgtgtgaa ggtagaaagt cctttccttt
      961 catctcgcca gctagaattc cacattttaa aatatgtaaa tgcttcaata agggtggaat
     1021 agattcatgt aaagaagctg gtaaaacact tgatcctaaa tgggtttcgg aatacacaaa
     1081 tgcatcggga ttagacatcc agtgtgcaat ttggttggga ataacctgtg gacggaaagc
     1141 aggtttgtga tagggatttt ctgtttcagc tgtttttcgt attgcagata aataaaatcc
     1201 ttcacctaaa atttgaccag gataaaaacg atatcccatg gacgttttta caatttccgg
     1261 atgattctct aaggatggaa tagcacagat ttctgctgca agttcttctt taatgaagct
     1321 actgatttgc tcatcttcgg cttctgaata actgcaggta gagtagagga gaagaccacc
     1381 ttcttttaaa gccggccaaa catcagttag gatgcgttct tgtcgagctg cacaatgagt
     1441 aactgcatct tccgaccaat ggctcatcgc cgctggatct tttctaaaca ttccggaacc
     1501 actgcatgga gcatcaatca caatgacatc aaaatatcct tctagttttc caaattggcg
     1561 aggatcgtta ttgcttacta tcgtattggc acttccccag cgatttaggt tctcgtgtaa
     1621 aattccagct cgactgcgaa tgacttcgtt ggcaactaac attccttcgc ctgctagcca
     1681 agatgctatc aaagtgcttt tgccaccggg tgctgcgcat aggtctaaaa cacgcaaatt
     1741 cgacttctca ggcactaaat cagtcaaagc cgaccaaata ctcatggagc tggcttcttg
     1801 cacgtaatac gcgcctgtat gaaaaagagg atcgagtgta aaatcgggtc ttgatggaag
     1861 atatcgtcca tgttcgcacc aagctacttt cgtttcgttt tcaaaggctg agttaagttt
     1921 ttcagggtta aggcggattg atactggggc tttttcttca tgtgctagaa gaaagctggt
     1981 tgtattgatt ccgggtagat cctggatatg ttcttgaaaa ccgagaggga aattggttgg
     2041 catgtggtaa aactaaaaaa gcaccccgag ttggagtgct tttgagaaag aaaggtgtca
     2101 gattatttca ccgcgtcgca gatagctttg aaagcttctg gattgttcat agccaaatcg
     2161 gcaagaactt tacggttaat ctggatgttt ttcgcgtgga ttttacccat gaattcgctg
     2221 tacgacattc cgtgttggcg aactccagcg ttgatacgct gaatccatag accgcggaaa
     2281 ttccgttttt tggttttacg gtcacggtaa gcataggtaa gacctttctc aagaatattc
     2341 ttcgctattg tgaagacgtt cttgcgggca ccccaatagc ccttggtctg gttgaggatt
     2401 ttctttctcc ttgcgcgtga ggcaacgtgg ttgactgaac gtggcatggt ttagttggtg
     2461 ttgtgatgaa cagcgttacg cctttcgggt aaacttagta ctgagcatct ggttaaaaaa
     2521 aattgatttt actttccaat cgttaacata cgtttaacga gaccttcatc agccttgcaa
     2581 accaaaccag aatggcctag acgacgcttc tgatcttttg atttcttggt gagaatgtgc
     2641 cgcataaacg cgtgcttgcg tttgattttt ccagaagccg tcagcttgaa gcgctttttg
     2701 gcactggaat ttgttttcat tttcggcatt gtgaattggt atttgggttt tacttatttc
     2761 ttctttttag gggagatata catgatcatc cgctttcctt ctagttcggg catgctttcg
     2821 gctttcccta attcttctag ctctgcagcg aatttcaaca agagaatttc tcctttttct
     2881 ttgaacaaaa tcgaacgtcc tttaaagaat acaaacgcct tcactttaga accttcttga
     2941 aggaatttgc gtgcatgatt caatttgaag ttgaaatcgt gctcatcagt attcggaccg
     3001 aaacgaactt ccttcaccac aatcttcgtg gctttggctt tcatttcttt ttgtttcttt
     3061 ttttgctcgt agaggaattt cttatagtca atcacccggc acacaggagg atctgcattc
     3121 ggaacaattt ccaccagatc taattcctgc tcacgtgcaa cacgtaaagc atcctcgaat
     3181 gagtaaacgg caggttcaat gccatctcct acgaggcgaa ctttgggtac accaactatt
     3241 tttaagttga tgcgatgtgc ttcttctttt ttctgcggtc cccggttaaa cggccgtgga
     3301 gagcgcggtg gcattgctat agcttgggtc ctcccttcag ttgtttgtta attcatttgc
     3361 ttgttaactt cctcttcgat taacgcagca aacgcttcta ggctcatggt gcctaaatcg
     3421 ccttcgccat gtttacggac agaaacagat cctgtttcag cttccttttc accaacaaca
     3481 agcataaaag gagtcttcat aatttcagca tcccttatct ttttgccggc acgttcgtca
     3541 cggtcgtcaa tagcggcgcg aatatcgtaa tttttaagca gttgtaaaac tttttttcca
     3601 tactcgttat acttctccga aataggcagt attactgctt gttcaggtgt taaccagagt
     3661 ggaaacttac ccgcacaatg ctctatcaag accgctacaa agcgttccaa cgacccaaaa
     3721 ggtgcacggt gaatcatcac cggacgatgt ttggcattat cagcacccat atattccaat
     3781 tggaaacgct ccggtaaatt gtaatctacc tgaattgttc cgagttgcca gcgccgacca
     3841 atagcatcct tcaccatgaa gtcgagctta ggaccataaa atgctgcttc tcctaattct
     3901 acaacagtac gcagtccttt ctcagcagca gcttcgataa ttgcattttc agctttctcc
     3961 caattttcgt cactaccaat atacttggtc ttgttttctg gatctcgcaa agaaatttga
     4021 gcggtgaaat ccttgaaatc aagtgctttg aaaatcataa acactagatc gattactttc
     4081 aagaattctt cttttacctg atcggggcga caaaaaatat gtgcatcatc ttgagtaaaa
     4141 ccgcgcacac gagtcaaacc atgtaattca ccactttgtt catagcggta aacggttccg
     4201 aattcagcta aacgaattgg aaggtcttta taggagtgtg gtttgttttt gtaaatctcg
     4261 cagtggtgcg gacaattcat cggcttcaag aaaaattctt ctccttcggc gggtgtttta
     4321 atgggttgaa acgaatcggc tccatatttc tcgtagtgac cagaggttac atacaagtct
     4381 ttatgtgcaa tatgtggagt gataacttga acatatcctg ttgaagcttg ggcgcgcttt
     4441 aagtaatcta ccaaacgctc acgtaaggca gttcctttgg gtaaccacat cggtaaacct
     4501 tgacctactt tttcggagaa catgaataac tccagctctt tccctagttt tcggtgatca
     4561 cgtttacggg cttcttccaa taaaacaagg tactcatcca aatcggcagc tttggtgaat
     4621 gtgataccat atatacgtgt aagctgtttg tttttctcat cgcctttcca atacgctcca
     4681 gcaacgttca tcaacttaac tgctttgatg aaacctgtat ctgggatatg aggaccgcgg
     4741 cataagtcgg tgaaattacc ttgttcgtaa aaggtaatgg tgccatcttc gagaccagct
     4801 aataagtcaa gtttatactc atcacctttc tctgtgaaat aagcaatggc atccgcttta
     4861 ctcatgtctt tgcggacata ctggcttttt ttcttagcca attctttcat tttagcttct
     4921 accgcaggaa gatcttcggc cgaaattgaa cgatcaccca aatcaacgtc gtagtaaaaa
     4981 ccattttcaa taggaggtcc aataccgaat ttaattcccg gatacagttc ttctaaagcc
     5041 tcagccatca agtgggccga actgtgccac atggttttct taccatcgtc atcgttccac
     5101 gtcaaaagct ttaccgttga atcggtttgg ataatgcgag tagcatcttg tacttctcca
     5161 ttcacagtgg cagcaaggac gttacgagca agtccttcac taatgctttt ggcaatgtcg
     5221 agggcagtta cgccactttc gtactggcga accgaaccat cgggcagggt aatattgatc
     5281 atagcttgta aaaattcagg gtggcaaaga taatcagaag aatgctaggt ttttattgaa
     5341 atgtgtgtgt gaattattta ctttggagaa aatctttgtc atgccttttt ctcgattatt
     5401 cctaattata agcttgatat tttcaagtcc taccctaata aacgcacaaa tccttagaga
     5461 acctattgta gaaccgactg aaccccctga tgtagatact aaggaacaag tgtttacaat
     5521 agttgaagaa atgcctgttt ttgaaaatgg aaaggatgat atcaatactt ttctagcaaa
     5581 aaacatcaat tatcctttgg aatgtaaaga gagtagtata caaggtaccg tcttcattaa
     5641 atttgtagtt caagctgctg gcggaattcg ggatattcag gttatgaaat cgcctaagga
     5701 gtgtaatatg ctagagaagg aagctatacg tgtagtaaag ctatctggta attattggaa
     5761 agctggaaaa caaaatggta aaaaagtaga tgtgtacaat attttaccga ttcgcttcaa
     5821 acttcaataa caacgaaaat tgtcatattt aaaaagagga aatcatgcgt tttttaatca
     5881 gctatctatt tttgctcgtt tcgttttgtg tggaaagcca aacggtaacc ccttcaaaaa
     5941 ctgatcgaat cgatgaacga agaacttctg atggtgaaag ctacccagtt caagatatta
     6001 ttccaactaa aagcgatatt tattctgttg ttgaagaaca ggctcattat aaatatggtg
     6061 atgaagcatt actgaaattc atttcaaaaa atattattta tcctccaagt gcagttgaga
     6121 ttgaaattca aggaaaagtg tatgtgcaat ttgtggtgaa tacagatagt acattgtcag
     6181 atatcactgt tgttcgagga ataaatggtg gtgaaatgtt aaataaagaa gcaattcgtg
     6241 ttgtcaagct cacttctggt gattgggttc cagc
//
LOCUS       DAOOQW010000133         6208 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_15370_length_6208_cov_4.407118, whole genome shotgun sequence.
ACCESSION   DAOOQW010000133 DAOOQW010000000
VERSION     DAOOQW010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 6208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6208
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15370_length_6208_cov_4.407118"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="PK281_08015"
     CDS             <1..294
                     /locus_tag="PK281_08015"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOY49330.1"
                     /translation="KTAKLVFTSLIRENPLFAPGWSNYGYVRLLEGFPEEAEYAYKKA
                     ISLNMDYVPAQLNLAGLYLYRGNKVDAKKILIRVLELEPKNSQAKEILTSLDE"
     gene            347..1435
                     /gene="mltG"
                     /locus_tag="PK281_08020"
     CDS             347..1435
                     /gene="mltG"
                     /locus_tag="PK281_08020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HOY49331.1"
                     /translation="MAKKRSSKSTRQKSSKGKSSSAKRIFIALGLILFLSVCISFGYF
                     YSKVFRSNLVLNQQKTDFVYIRTIDSFQDVVNQLTERGMLRNRESFIWLSEFMDYDKK
                     VKAGRFKVKDGMSNRELVMLLRSGKQEPIRITFQNIRTKEDFASKVGRNLEADSSSLM
                     DILNNNEEMRQFGLDSQHALSLFLPDTYEFYWNTTAPVFVEKMGKAYTKFWTEDRLSK
                     ATQLGLSRGEICVLASIVLQESNKSDEWPVIAGVYLNRLHKGMKLQADPTVKFALNDF
                     GLRRIRSNHLQVESPYNTYKYAGLPPGPIYMASKGSIDAILNYQTHSYLYFCARPDRS
                     GYHSFAETFEQHKQNARRYQSSLNDRGI"
     gene            1447..2853
                     /locus_tag="PK281_08025"
     CDS             1447..2853
                     /locus_tag="PK281_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HOY49332.1"
                     /translation="MTKIKFGTDGWRAIIADEFTVENVARVSEATAIWLKKNSSSPSV
                     VIGHDCRFAGELFAETTAKVMICHGVKVFLAKGFVSTPMVSMGAITYKTNLGIVLTAS
                     HNPPSYNGFKLKGSYGGPLLPDKIDEIETIIPETANSAWQKVSLTEAEQAGTCVIVDL
                     EGDYCQRVESSFDLDAIRNSNLEFAYDAMFGAGQNVIRRLLPNTTLLHCDYNPSFEGQ
                     APEPIAKNLTEFSEMIRLSGNIDCGLATDGDADRIGLFDSEGKFVDSHHIILLLIHYL
                     KKYKGLDGKVCTAFSSTVKVKTICDHYGLDLDVVKIGFKYIAGIMITDDVLLGGEESG
                     GIAIKGHIPERDGIWMGLVIWEFMAKSGKTLDQLIEEVYEITGAFSVDRNDLHLPEAE
                     KQRVITSCKNGEVKNIGDRPVIRVEDMDGFKYFVSEDEWLMVRPSGTEPLLRVYAEAH
                     DHAGVMKLIGDARKQFGI"
     gene            2850..3758
                     /locus_tag="PK281_08030"
     CDS             2850..3758
                     /locus_tag="PK281_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49333.1"
                     /translation="MKFNIWLPVLSFCFLSGSTFAQAQVNSTIEVEAQGTITFSPDSF
                     VVKLYMDYSSVNYSDRLADEGFSNEIVEMPVDMEVSEMDYATVVEAEEPPPAIEEVKS
                     PEQLRNEHERYRRVEDSLQSIKAKRLLEFMAKLKTMGVGFSKDDSEQSRYSYRSNSWV
                     QNFSPAQYGKIDSLSRLYGHPLHLEMVDIKMKDHSVLKERSYRQAIENSKKEADILAK
                     AMGRKLGKLTTVKTEAMDVMDLVPLMLRKEIQREIRKSDRFIQPALYQLSQEFGREMF
                     EKDRYDDKKIVYWTWTEKIHVTYQLN"
     gene            complement(3980..4327)
                     /gene="rplS"
                     /locus_tag="PK281_08035"
     CDS             complement(3980..4327)
                     /gene="rplS"
                     /locus_tag="PK281_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010523444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HOY49334.1"
                     /translation="MDFLSQIASQLTEKRELPAFKAGDTITVHYKIKEGEKERIQQYQ
                     GVVIQRKGAGVTRTFTVRKISNSLGVERIFPDNSPFIDKIEVNKRGVVRRARIFYIRD
                     LKGKKARIKEKMV"
     gene            complement(4407..5084)
                     /gene="trmD"
                     /locus_tag="PK281_08040"
     CDS             complement(4407..5084)
                     /gene="trmD"
                     /locus_tag="PK281_08040"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010517983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOY49335.1"
                     /translation="MEIHIITVLPELLESPFAHSILKRAQQKELVEVHIHNLRDYSLD
                     KHKSVDDYAFGGGAGMVMMMDPIVRCIEHLQSKGKFDEVLYMTPDGERFNQSIANQMS
                     TLKRIAILCGHYKGVDERVREHFITREISIGDYVLSGGELAAAVVSDAIIRLIPGVLN
                     DETSALTDSFQDNLLAPPVYTRPAEYRGWKVPDILLSGNTPAVDKWRFEQSLERTRIR
                     RPDLLDE"
     gene            5185..6180
                     /gene="bioB"
                     /locus_tag="PK281_08045"
     CDS             5185..6180
                     /gene="bioB"
                     /locus_tag="PK281_08045"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013620609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HOY49336.1"
                     /translation="MNENRPQVSKEEILDIYQKPLLELVFEAATIHRQFNVGSEVQVS
                     SLISIKTGGCSEDCGYCPQAARYSTGLEAHKLMSVEQVTEYANRAKEGGASRVCMGAA
                     WREVRDNRDFDRVIEMVQAVNSMDMEVCCTLGMITEHQAQRLADAGLYAYNHNIDTSE
                     ENYSNIISTRNFDDRLNTIKNVRKANITVCSGGIIGLGETIEDRCGMLHTLVNLPVHP
                     ESVPINALVAVEGTPLENQPPVPIWDMVRMIATTRILMPKTMVRLSAGRQQMSLEGQA
                     LCFLAGANSIFTGEKLLTTPNPEYDTDMEMFKLFGLTPKAPFKDKKKIAVEQAVD"
BASE COUNT         1965 a         1011 c         1312 g         1920 t
ORIGIN      
        1 aaaacagcca aattggtttt tacatctttg attcgtgaaa atccattgtt tgctccagga
       61 tggtcaaatt atgggtatgt tcgattgtta gagggatttc ctgaagaagc agaatatgca
      121 tacaagaaag ctatttcctt aaatatggat tatgttcctg cacagttgaa cttggctggg
      181 ctttatttat atcgagggaa taaagttgat gcaaaaaaaa ttttaatacg agttttagaa
      241 ttggaaccaa agaatagtca agctaaggag atactcactt ctcttgatga atagttaatc
      301 ataccgtttg aaatcttcat gaaaccagta attttggtgt cattatatgg ctaaaaaaag
      361 aagttctaaa tctacccggc aaaaatcaag taaaggaaaa tcgagttctg ctaaacgtat
      421 ttttatagcg cttggcttga ttttgttttt atcggtatgc atttcatttg ggtattttta
      481 ttctaaagta tttcgttcta atctagtatt gaatcaacag aaaacagact ttgtatacat
      541 acgaaccata gatagttttc aagatgtagt aaaccagctt accgaaaggg gaatgctcag
      601 gaatagagag tcttttattt ggctttctga attcatggat tatgataaga aggtaaaggc
      661 tggtaggttt aaggtaaaag atggaatgag taatcgtgaa ttggtcatgt tacttcgatc
      721 aggtaagcag gaaccaatac gaattacttt tcagaacatt cggactaagg aggattttgc
      781 tagtaaagtt ggaaggaatt tagaagctga ctcatcatca ttaatggata ttttgaataa
      841 caatgaagag atgaggcagt ttggattgga ttcacaacat gcattatcgt tgtttttacc
      901 agatacatat gagttttatt ggaatactac agctcctgtt tttgtcgaaa aaatgggaaa
      961 agcttatact aagttttgga cagaagaccg attaagcaaa gcaacacaac ttggattatc
     1021 tagaggagag atttgtgtcc tagcatctat tgtacttcaa gaatcgaata aatcggatga
     1081 atggccagtt atagcaggcg tttatctcaa tagacttcat aaaggaatga aattgcaagc
     1141 agatcctacc gtgaagtttg ctttaaatga ttttgggtta cgtaggatac gatctaatca
     1201 tcttcaggtc gaatcgcctt ataacactta taaatatgca ggtttaccac cggggccaat
     1261 ttatatggct agtaaaggaa gtattgatgc aattttgaat tatcaaacac atagttacct
     1321 ttacttttgt gcccggcctg accgttcggg ttatcattct tttgctgaaa cattcgaaca
     1381 acataaacaa aatgcacgac ggtatcaatc aagtttgaat gaccgcggaa tttaaaaaga
     1441 caaattatga caaagattaa gtttggaaca gatggctgga gagccattat tgctgatgaa
     1501 tttacagtag aaaacgttgc tcgtgtatcg gaagcaacag caatctggct aaaaaagaac
     1561 tcgtcttcac cttcggttgt aataggtcat gattgtaggt ttgctggaga gttatttgcc
     1621 gaaaccacag ctaaggttat gatttgccat ggagttaaag tatttcttgc aaaaggcttt
     1681 gtatcaactc ctatggtttc gatgggagct ataacttata aaactaactt aggaattgtt
     1741 ttaacggcaa gccataaccc accttcttat aatggtttta aacttaaagg aagttatggt
     1801 ggtccattat tgccagataa aatagacgag attgaaacca tcatcccaga aacagcaaat
     1861 tcagcttggc aaaaagtttc acttactgaa gctgaacaag ctggtacatg tgtaatagtt
     1921 gatttggaag gagattattg tcaacgtgtt gaatcaagtt ttgatttaga tgccatccga
     1981 aattcaaatc tcgagtttgc ttatgatgct atgtttgggg ctggacaaaa tgtaatacgt
     2041 cgtttactac ccaacaccac tttattgcat tgtgattaca acccttcttt tgaaggtcaa
     2101 gcgcccgaac ctatcgctaa aaatctaacc gagttttctg aaatgattcg tctttcagga
     2161 aatattgatt gtggattagc tactgatggt gatgccgatc gtattggact ttttgatagt
     2221 gaagggaaat ttgttgattc acatcacatt attcttttgt tgattcatta tttgaaaaag
     2281 tacaaaggat tagatggtaa agtatgtact gcattctcct cgaccgttaa agtgaaaacg
     2341 atttgtgatc attacggatt agatctcgat gtggtgaaaa ttggatttaa atacatcgct
     2401 ggaattatga tcactgatga tgtattatta ggtggtgaag aatctggtgg tattgccatt
     2461 aaaggtcata ttccagagcg tgatggaatt tggatgggat tggttatttg ggaatttatg
     2521 gccaaatctg gaaaaacact ggatcaacta atcgaagaag tttatgagat aactggtgca
     2581 tttagtgttg atcgtaatga tttacacttg ccagaagctg aaaaacaacg tgttattaca
     2641 tcttgtaaaa atggagaggt taagaatatt ggagatcgtc cagtgattcg agtagaagat
     2701 atggatggat ttaagtattt cgtcagcgaa gatgaatggc tcatggttcg tccatctgga
     2761 acggaaccgc ttttaagagt ttatgccgaa gcacatgatc acgctggtgt aatgaaactt
     2821 ataggtgatg ctcgtaagca atttggtata tgaaatttaa tatttggctt ccagttctaa
     2881 gtttctgctt tttgtcgggt agtacttttg ctcaagctca ggtaaattct acaatagaag
     2941 tagaagcaca aggaactata acatttagtc ctgatagttt tgttgttaaa ctatacatgg
     3001 attattcttc tgttaattac tctgatcggt tggcagatga gggattttcc aatgaaattg
     3061 tcgaaatgcc tgttgatatg gaagtgtcgg aaatggacta tgctactgta gttgaagccg
     3121 aagaacctcc tcctgctata gaagaagtaa aatcaccaga gcaattacgt aatgaacatg
     3181 aaagatatcg acgtgtagaa gattctctac aatccataaa agcgaaacgc ttgctcgaat
     3241 tcatggcaaa gctaaaaaca atgggagttg gcttctcaaa ggatgattcg gaacagtcga
     3301 gatatagtta tcgttctaat tcttgggtgc aaaatttcag tcctgctcaa tatggtaaaa
     3361 tagattcgtt gagtcgttta tatggacatc cattgcattt ggaaatggtt gatataaaaa
     3421 tgaaagatca ttctgttttg aaagaacgtt cataccgtca agctattgag aacagtaaga
     3481 aagaggcgga tattttagcc aaagcaatgg ggcgtaaatt aggtaagttg accactgtca
     3541 aaacagaagc tatggacgta atggatttag ttcccttgat gttacgtaag gaaattcagc
     3601 gtgagattcg taagagtgat cgattcattc aacccgcatt atatcaactt agtcaagaat
     3661 ttggtagaga aatgtttgag aaagatcgtt atgatgataa aaagatagtt tactggactt
     3721 ggactgaaaa gattcatgtg acttatcagt tgaactagct aaaatttaga atgaatattg
     3781 aaatcccggt tgcatatgta atcgggattt tttttgtctc cttttgaacg caactcgaac
     3841 tcttcatcaa acattctatt ggtaaaatga tatactataa atgataagtt gatatgctga
     3901 atattctagt aatatataaa aagaaagccc tgttgaatga acaacagggc ttttatatgt
     3961 gtgcaaagaa taggatgaat taaaccatct tttctttgat acgcgctttt ttacctttaa
     4021 gatcgcggat gtagaaaata cgagcacgac gaacaacacc acgtttattc acttcgattt
     4081 tatcgatgaa tggtgagtta tcaggaaaga tacgttcaac tcctaagcta ttagaaatct
     4141 tacgtactgt gaaagtgcgt gtaactccag cacctttacg ttgaataaca acgccttggt
     4201 attgctgaat acgttctttt tcaccttcct tgattttata gtgaacggtg attgtatctc
     4261 cggctttgaa agcgggaagc tcacgttttt cggtaagctg actggcaatt tgtgaaagga
     4321 agtccattgt gattttccta cgggtttcta aaaaggagtg caatattagg gaataatttc
     4381 attcaaacag caaacaaatg aaaaaattat tcatctaata aatcaggtcg ccgtattcta
     4441 gttcgttcga gtgattgttc aaagcgccat ttgtcgactg caggcgtgtt tccactaagt
     4501 aatatgtcgg gaactttcca tcctctatat tctgcagggc gtgtataaac aggtggagcc
     4561 aataagttat cctgaaagga atcggtcaaa gcagaagttt catcattcaa aactcccggt
     4621 attaatcgga ttatagcatc agaaacaaca gcagcagcta attctcctcc tgaaagtaca
     4681 taatcaccaa ttgaaatttc acgcgtgata aaatgttccc gaactcgttc gtcaacccct
     4741 ttataatgcc cacacaagat tgcaatacgt tttaaagtag acatttgatt ggctatcgat
     4801 tggttaaatc gttcaccatc aggagtcata taaaggacct catcaaactt ccctttcgat
     4861 tggagatgtt caatgcatcg aacaattggg tccatcatca tcaccattcc agctccgcct
     4921 ccaaaagcat agtcgtcgac acttttatgt ttatcgagcg aatagtctct caaattatgg
     4981 atgtgaacct caacgagttc cttttgttga gcacgtttca aaatagaatg tgcgaaagga
     5041 ctttcgagaa gttcgggtag gacagtaata atgtgaattt ccatggatgg cgaaattaag
     5101 agatttagga aagcgatgat tgggtacatt tgtccccgtt tgatccatac tcattcatta
     5161 tatttgttag cattaagcat gttcatgaac gaaaatcgtc cccaagtaag caaggaagaa
     5221 atccttgata tatatcagaa accacttttg gaattggttt ttgaagccgc aactattcat
     5281 cgtcaattca atgtaggatc ggaagttcaa gttagttcgc tcatctccat taaaacaggt
     5341 ggttgttcgg aggattgcgg atattgtcct caggcagcgc gctattccac cggattggaa
     5401 gcacataaat taatgagcgt tgagcaggtt actgaatatg caaaccgtgc aaaagaagga
     5461 ggagcttctc gtgtatgtat gggagctgct tggagagaag ttcgcgataa tcgtgatttt
     5521 gatcgggtga ttgaaatggt acaagccgtg aattctatgg atatggaagt gtgctgtaca
     5581 ttaggaatga ttaccgagca tcaagctcaa cgtcttgctg atgctggcct atatgcctac
     5641 aatcacaaca ttgatacatc ggaagagaat tatagtaata tcatttcaac ccggaatttt
     5701 gacgatcgtt tgaacacgat taagaacgtg cgtaaagcga atattacagt ttgttcaggt
     5761 ggaattattg gattaggtga aacgattgaa gatcgttgtg gtatgcttca taccttagtt
     5821 aatcttcctg ttcatccaga atcggttcca attaatgctt tagtagctgt tgaaggaaca
     5881 cctctagaaa atcaaccacc tgttccaatt tgggatatgg ttcgaatgat tgctactacc
     5941 cggatcttga tgcctaaaac aatggttcgt ctttctgctg gtcgtcaaca gatgtcatta
     6001 gaaggacaag cattatgctt cctagctgga gcaaattcca tctttacagg tgaaaagctt
     6061 ttaacaacgc cgaatcctga atatgataca gatatggaaa tgtttaagct ctttggatta
     6121 accccgaaag caccatttaa ggacaaaaag aaaattgcag tagagcaggc agttgattaa
     6181 tacaacaaat taaggtgtat gaaagtgc
//
LOCUS       DAOOQW010000134         6166 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_15513_length_6166_cov_4.364425, whole genome shotgun sequence.
ACCESSION   DAOOQW010000134 DAOOQW010000000
VERSION     DAOOQW010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 6166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6166
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15513_length_6166_cov_4.364425"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1063)
                     /locus_tag="PK281_08050"
     CDS             complement(<1..1063)
                     /locus_tag="PK281_08050"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprB repeat-containing protein"
                     /protein_id="HOY49337.1"
                     /translation="MKYFTFLNKGLLTLVAFLTGAMALQAQTGLTISMADSNGVSCQD
                     DAYATINVSGGVGPYTAYWLRYIDGVSNPPGPLGVQIDTVSIGLSADSLAPGYYQIHV
                     YDSSIPTPILGYYGVYIQGSFSVSQVTTPATCTNADGKIKLTVLNTVGPYSFDWSTGV
                     QHNGLTANSDSVMNLLAGYYSCTVTDGNGCYVSAGGAGTTQGGLYVYATSPIVVTGSA
                     TPSNCYDGTATVVATLGTAPYSYVWSTTPAQFGVTATGLSPGYVFCTVTDAVGCSRQQ
                     YVSIPAGPNYLQATSNISQEICGGTPGAIDLTVTGGVTPYTYLWSNGSTSQDQTGLSS
                     GSYSVVISDAQGCSLTAYKY"
     gene            1319..2971
                     /gene="ade"
                     /locus_tag="PK281_08055"
     CDS             1319..2971
                     /gene="ade"
                     /locus_tag="PK281_08055"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HOY49338.1"
                     /translation="MQNNHSFRITARRIDLLGSSISASTIVVENGIITAIEPADEADC
                     NTFLLPGFIDAHVHVESSLLIPSEFARMAVAHGMVGTISDPHEIANVLGVFGVEYMLK
                     NAARVPFHFFFGAPSCVPATVFETAGDTINPAQVEYLLKRPDIWYLAEVMNYPGVLAG
                     DEDMLAKIDAAKRCGKPIDGHAPGLRGDDVVRYAAAGITTDHECFTLDEALDKIKAGM
                     WVQIREGSAARNFDALHTLLGSHPERVMLCSDDKHPDNLLEGHINVQVKRALSLGYNL
                     FDVLRASSWNVKQHYNLPVGMLQVGDSADFIEIDSIEQFNILGTWIQGEQVASKGKTN
                     IHHHEERPENRFHSREVKLEELFFPAQNGQLRVIEALEGQLITRELHLPPRINEEGNV
                     LSQPDQDILKIVVVNRYHDAPPAIAFIRNFGLKKGAIASTVAHDSHNIIATGCTDEEI
                     LRAINLLMLSKGGLCAVDKEEDHLLALPVAGLMSNDDGWEVAKRYIHLDTFVKEKLGS
                     KLRAPYMTLSFMALLVIPQLKLSDKGLFDGSTFRFTSNWAEA"
     gene            complement(3013..3273)
                     /locus_tag="PK281_08060"
     CDS             complement(3013..3273)
                     /locus_tag="PK281_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49339.1"
                     /translation="MEETVKAKNISPLFNRMAYGLFILLSIYFAVFSKNYPSALSNFG
                     IALVFDPFDQTVRFPDRPLYQKVWLITHASLALIGFVLMLVL"
     gene            complement(3330..3674)
                     /locus_tag="PK281_08065"
     CDS             complement(3330..3674)
                     /locus_tag="PK281_08065"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HOY49340.1"
                     /translation="MRIKLFSVFISLIFFAACNGVSTPPSAKTAEFKVWGNCGMCKKT
                     IEGSLKDKPGIIKADWNVKSKQMSVQFDTLRISLKDIQQTIAMAGYDNDAFQGDSAAY
                     AGLHECCQYTRK"
     gene            complement(3671..4735)
                     /locus_tag="PK281_08070"
     CDS             complement(3671..4735)
                     /locus_tag="PK281_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015481356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HOY49341.1"
                     /translation="MRKTAYLLSICLGLFMLLEACSKDESVYSTNTENPGPTPYNLVI
                     PQGLPAMVIPADNPLTVEGIALGKKLFFDPILSGDNSISCGTCHLQEFGFTDSLRQFS
                     KGIDHIEGTRNSMPLINLGWERNFFWDGGASNLESQVAGPITNPVEMHETLANAVAEL
                     NAHPEYPALFKKAFGSEIVNTANVMKAIAQFERTLISGNSRFDKYMQLGIPLTEQELR
                     GRDVFISFFKGDCVHCHVMGSTFTDHEFRNNGLDTFSVDLGRGRITLNPSDNGKFKTP
                     GLRNIAVTAPYMHDGRFATLEEVINHYNNGIQPHPNLDPRLVDTQPGRMSEQDVEDII
                     AFLHTLTDEEFLTNPAFKKP"
     gene            complement(4732..5493)
                     /locus_tag="PK281_08075"
     CDS             complement(4732..5493)
                     /locus_tag="PK281_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49342.1"
                     /translation="MHTRSCFLAIILSVSILGGCKKDEEEKPTPTPADTTGSVAFQVS
                     NRIGNQDLALGYLSYVNAAGDSFQVDLLKYYISGFEWKRADGTYFQSKNYQLINTSQP
                     TSLNFSLTGIPNGTYTGLRFYLGVDSLSNHTITNYVALDASSGMIWSWNTGYIFFKHE
                     GKYIDSNHVEKPLLFHYGTDRGFATVELPLDNFVVNSDSRQLNLTFDLAALYANPNMI
                     DFNIWNNNQSLTTQEYPWLDAMKQNFSQSFSVHVP"
     gene            complement(5535..>6166)
                     /locus_tag="PK281_08080"
     CDS             complement(5535..>6166)
                     /locus_tag="PK281_08080"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY49343.1"
                     /translation="NGHQKVFRMNIETGVFSAQLPAYGPHETMAEYLKVTGYTWENVV
                     TTGLQDPAGIAIQGNRMVISDHQSSDLIFYDITSVPATELHRISTGAQGIMGITIGPD
                     GKIYYVENTGNKVVRLDPETIPLKVPIIENSWTVFPNPSNGILAIRGAEMHVPIQVQV
                     YASNGQLLRNYTTETGQLKDTGLRSGMYHIRVLDLAGKELSQFSWVIAQ"
BASE COUNT         1844 a         1248 c         1274 g         1800 t
ORIGIN      
        1 catatttgta agctgttagc gaacaacctt gcgcatcaga aataactacc gaataacttc
       61 cacttgacaa acctgtttgg tcttgtgagg tgcttccatt tgaccacaaa tatgtgtatg
      121 gagttactcc tccagtaaca gttaaatcaa tagctccagg tgttccacca caaatttctt
      181 gtgaaatatt acttgttgct tgtaaataat ttggaccggc tggaattgaa acatattgtt
      241 gacgtgaaca acccacagca tcggtaactg tgcaaaacac ataacctgga gatagtccag
      301 ttgctgtaac accaaactgc gctggtgttg tactccaaac ataggaataa ggtgcagttc
      361 ctaatgtagc gactactgtt gcagttccat cataacaatt agatggtgta gctgagcctg
      421 ttacaacaat gggtgaagta gcatatacat acaagccacc ttgagttgtt ccggcaccgc
      481 cagcactaac ataacatcca tttccatcgg taacggtaca ggaataatat ccagctaaaa
      541 gattcatcac ggaatcggaa tttgctgtta atccgttatg ctgaactcct gtactccaat
      601 cgaaactata tggcccaaca gtattgagaa ccgttagttt aatcttacca tcagcatttg
      661 tacaagtagc aggtgttgta acttgactca ccgaaaatga tccttgtata taaactccat
      721 aataacccaa aattggagtt ggtatggatg aatcatacac atgaatctga taatatccag
      781 gtgctaaact atcagcactc aaaccaattg aaaccgtatc gatttgtaca cctaatggcc
      841 ccggaggatt cgatacacca tcgatatatc ttaaccaata agccgtgtat gggccaacac
      901 caccgctaac attaatggta gcataggcat cgtcttgaca agaaactccg ttggaatctg
      961 ccatgctaat tgtcaaacct gtttgtgcct gcaatgccat agcgccggtt aggaaagcaa
     1021 ctaaggtgag aaggcctttg ttaaggaaag taaagtactt cataatagat agttagaaat
     1081 ttagggggaa tttacttctt ctttttgaat gacctacttt tgttcgagtt aatctataca
     1141 acatcttacg tacttatacc agaattgaat ctttaaatcg tttactggct tgaatttcag
     1201 ttgataattt caaacgcatt gagctcaaca aaaaactgga ttttttaatt tcatagtacc
     1261 tgaaaagttt ccattaaaag atgaattgaa actgcgttta aggtgttagg tttggaacat
     1321 gcaaaacaac catagctttc gcattacagc tcgccgaatt gatttactcg gtagtagtat
     1381 ttcggcatcg accattgtag ttgaaaatgg tatcataacg gcaattgaac cagcagatga
     1441 agccgattgt aacacgtttc ttttgcccgg atttattgat gcacacgttc acgtagaaag
     1501 tagtttgctc attccatccg aatttgcgcg tatggcggtg gcacatggta tggtaggaac
     1561 catttctgat ccgcatgaga tagctaatgt tttgggcgtt tttggtgtcg aatacatgct
     1621 gaaaaatgcc gctcgcgttc cgttccattt tttctttgga gcaccatcgt gcgttcctgc
     1681 aacggttttc gaaactgctg gtgatacgat caatcctgca caagtagagt atttacttaa
     1741 gcgacctgat atttggtatt tagctgaagt gatgaattat ccgggagttt tggcgggtga
     1801 tgaagatatg ttggccaaaa ttgatgctgc taaacgctgc ggaaaaccta tcgatggaca
     1861 tgcgccgggt ttacggggtg atgatgttgt tcgctatgct gctgcaggaa tcacaaccga
     1921 tcacgaatgt ttcaccttag atgaagcgct cgataaaatt aaagctggaa tgtgggttca
     1981 aattcgggaa ggaagtgccg ctcggaattt tgatgcattg cacacattac ttggctcaca
     2041 tccagaacgt gtcatgcttt gcagcgatga taaacatccc gataatttac tcgaaggtca
     2101 tataaacgtt caagtaaaac gtgccttgtc attaggttac aacttgtttg atgtgttacg
     2161 tgcctcttcc tggaatgtaa aacaacatta taatttaccc gttggaatgc ttcaagtggg
     2221 cgattcggcc gattttattg agattgattc aattgagcag ttcaacattt taggaacttg
     2281 gattcaaggt gaacaagttg cttcgaaagg aaaaacaaat attcatcatc acgaagaacg
     2341 ccccgaaaac cgcttccaca gccgtgaagt gaaattagaa gaactatttt tccctgctca
     2401 aaacggacag ttacgtgtta ttgaagcgct cgaaggacaa ctgattacac gcgaacttca
     2461 tcttcctccg cgtatcaacg aagagggaaa tgtgcttagt cagcccgatc aagatatatt
     2521 gaaaattgtt gtagtaaatc gctatcacga tgctccgcca gctatagcat ttatacgaaa
     2581 tttcggattg aagaaaggtg ctattgcatc tacggtggca catgatagtc acaacattat
     2641 tgcgactggt tgtaccgatg aggaaattct acgagccatt aatttactta tgcttagcaa
     2701 gggcggatta tgtgccgttg acaaagaaga agatcatcta cttgccctac cagttgcggg
     2761 attgatgagt aatgacgatg gctgggaagt tgccaaacgc tacattcatt tggatacctt
     2821 tgtgaaagaa aaactgggaa gcaaactgcg cgctccgtat atgaccttgt cctttatggc
     2881 tttactggta attcctcaac taaaactaag cgacaaagga ttatttgacg gtagcacgtt
     2941 tcgctttacc agcaattggg ctgaagcctg agttagtagc tttgcgagcc gtagctttag
     3001 cgaaggatca acttacaaaa ccaacatcaa tacgaaacct atcaaagcta aacttgcgtg
     3061 ggtaattagc cagacctttt gatatagcgg acgatctggg aatcttactg tttgatcgaa
     3121 tggatcgaac acgagtgcaa tgccgaaatt acttaaggca gaaggataat ttttcgagaa
     3181 aactgcaaag taaatactga gcagaataaa taatccatac gccattcggt taaaaagggg
     3241 cgaaatgttt ttggctttaa cagtctcttc catgagcttt tttactattt agtagcttag
     3301 tagaaaaaac tattacagaa gagtagaaat tattttctag tgtactgaca gcattcgtgc
     3361 aaaccggcat aggcagccga atctccttga aaagcatcat tgtcgtatcc tgccatggca
     3421 atggtttgct gaatgtcttt caaggaaata cgcagcgtat cgaattgcac cgacatctgc
     3481 ttcgatttta cgttccaatc ggctttgata attccaggtt tatctttcaa ggaaccttcg
     3541 atggtctttt tgcacatacc gcaatttccc cagactttaa actcagctgt tttagccgat
     3601 ggcggagtgg aaacaccgtt acaagcagcg aaaaatatca gggaaataaa aacagaaaac
     3661 agtttaattc tcatggcttt ttaaaggctg gattggtgag gaattcttcg tcggtaaggg
     3721 tatgtaaaaa tgcgatgata tcttccacat cttgttcgct catgcgtccg ggttgagtgt
     3781 caactaagcg cgggtcgagg tttggatgag gttgaattcc attgttataa tggttgatga
     3841 cttcttcgag cgttgcaaat cttccatcat gcatatatgg tgctgttaca gcaatattac
     3901 gcaatccggg agttttgaat ttaccattat cggaaggatt taaagtgatt cgtccgcgac
     3961 ctaaatcaac cgaaaatgta tctaaaccat tgttgcggaa ttcgtgatcg gtaaaggtgc
     4021 tacccatcac atgacaatgc acgcaatcgc ctttgaagaa gctaataaag acatcccttc
     4081 cacgcagttc ctgttcggtt aatggtatac ctaattgcat gtatttatcg aaacgagaat
     4141 tacccgaaat caaggttctt tcgaattgag caatggcttt catcacattg gctgtattga
     4201 cgatttcgga gccaaatgct tttttaaata atgctggata ttcgggatgt gcattgagct
     4261 cagcaacagc attggcaagc gtttcatgca tttcgacagg attggttatt ggtccagcta
     4321 cttgcgattc taaattgctt gcaccaccat cccaaaagaa atttcgttcc caaccgagat
     4381 ttataagcgg catcgaattt ctcgttcctt caatgtggtc gattcctttg ctgaattgtc
     4441 ttaagctatc tgtaaatccg aactcctgca agtgacaggt tccgcaggaa atagaattat
     4501 cgccagaaag aataggatcg aaaaacaact ttttccctaa tgcaattcct tcaaccgtta
     4561 atggattatc ggctggaata accattgccg gcaaaccttg tggaatgact aaattatatg
     4621 gtgttggtcc tggattttcg gtattagtag aatagactga ttcatcttta gaacacgctt
     4681 ccaagagcat gaataagcct aaacaaatcg ataagaggta agccgtcttt ctcatggtac
     4741 gtgaacagaa aagctctgtg aaaagttctg tttcatggca tctaaccaag gatattcttg
     4801 ggtagtaagc gattgattat tattccagat attgaaatca atcatattcg gattcgcata
     4861 caaagccgct aaatcgaaag taagattgag ctggcgcgaa tcggagttaa ccacgaaatt
     4921 atctaaagga agttctacag ttgcaaatcc acgatcggtt ccataatgaa acaaaagcgg
     4981 cttttctaca tggttcgaat cgatgtattt tccttcgtgt ttgaagaaga tataacccgt
     5041 attccaactc caaatcatcc cagaagatgc atccaatgct acataattgg ttatggtgtg
     5101 attgctaagt gaatcaactc ctaaataaaa acgcaaacca gtatatgtac cgtttgggat
     5161 gcctgtcaaa ctgaaattta aactggtagg ctgtgaggta tttattaatt gataattctt
     5221 ggattgaaaa taagttccat cagctcgttt ccactcaaat ccactgatgt aatatttaag
     5281 taaatccact tgaaacgaat cgccagcagc attgacgtat gacaaatatc ccaaagccaa
     5341 atcctgattt ccgattcggt tgctcacttg aaaagcaaca gaaccagtag tatcggcagg
     5401 agttggagta ggtttttcct cctcatcttt tttacatcca cctaaaatgg aaaccgaaag
     5461 tatgattgcc aaaaagcagc ttcgagtgtg catgaaattc atcatgcgaa ggtataaaaa
     5521 gccagcttat tctattactg cgcaattacc caagaaaact gcgaaagctc ttttccagct
     5581 aaatctaaaa cacgaatgtg atacattcca gatcgcaatc cggtatcctt caattgtccc
     5641 gtttcggtag tgtaatttcg aagaagttgt ccgttagaag catacacctg aacctgaatc
     5701 ggaacatgca tttcagcacc tctgattgct aatattccat tcgaaggatt cgggaaaacg
     5761 gtccagctat tctcaataat tggaactttc aacggaatcg tttctggatc taaacgaacc
     5821 actttgttac cggtattttc cacataataa atcttcccat caggaccaat cgtaatgccc
     5881 ataatacctt gcgcaccggt tgaaatacga tgcaattcgg tagctggaac actcgtgata
     5941 tcatagaaaa tcaaatcgct gctttggtgg tcgctaatca ccattcggtt tccttgaatg
     6001 gcaattccag ctggatcttg caagccagtt gtgactacat tttcccacgt atagccagta
     6061 actttgaggt attcagccat ggtttcgtgc ggaccgtaag ctggtaattg tgctgaaaaa
     6121 actccggttt caatattcat tcgaaatacc ttttgatgtc cgttat
//
LOCUS       DAOOQW010000135         6157 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_15542_length_6157_cov_6.232874, whole genome shotgun sequence.
ACCESSION   DAOOQW010000135 DAOOQW010000000
VERSION     DAOOQW010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 6157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6157
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15542_length_6157_cov_6.232874"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..785
                     /locus_tag="PK281_08085"
     CDS             <1..785
                     /locus_tag="PK281_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HOY49344.1"
                     /translation="GMKWMMGWMHDTLRYFKTDPLYREHHQNDVTFSIYYAFTESFML
                     PLSHDEVVHGKGSLIDRMPGDQWQKFANLRAMYSYMFTHPGSKLLFMGDEFGQYSEWN
                     YKTGLDWHLTGFEPNSGMQKLIKDLNTLYRKEPALHSLQFDTKGFEWIDLNDSRNSTL
                     SFIRRGEKPEDYLVIACNFTPIPQENYRIGVPDGGKYSVIFNSDNKSYWGSDYSAKHK
                     YESTDVEKHGRPHSIAIDLPPLSVQVFRLTARHKKAAVKKKA"
     gene            838..2352
                     /locus_tag="PK281_08090"
     CDS             838..2352
                     /locus_tag="PK281_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002661873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HOY49345.1"
                     /translation="MKQEFITAVQSSYPDSQAHIFLGCGVLKGEILSDARVNIPLSMM
                     NRHGLIAGATGSGKTRTLQVLAEQLSKAGVPVFLSDIKGDLSGLGKPGVSNAKIEERA
                     SALNINFQPEGVPIELYSLSGKLGAQMRATVMEFGPVLLAKILELNETQTGVLSILFK
                     YADDKGLPIVDLQDLKKVLNYLSEGPGKAEIKESYGIISSATSGTILRKIVALEQQGV
                     AAVFGEPSFDISDLMLKVDGQGVVSLLNVSDMQDKPAVFSTFLLSLLVELYETLPEAG
                     DLDKPKLVFFLDEAHLLFSDAPKAFLEQVEQIVRLIRSKGVGVFFCSQMAQDIPVNVL
                     AQLGNRFQHVLRAFTPNDAKALKDTVKTYPRSAFYEIEETLTQMGIGQALVTVLNSKG
                     IPTEVVHTHLVPPAAIMGPLPASEYQDHLNQSELFAKYAETTDPVSAYEMLNSRLQKQ
                     QEETSTTSSTPPKSGKQEKTMLEEVMNSSVTRSIGRELVRGIFGVLFGSSKRRR"
     gene            complement(2353..2805)
                     /locus_tag="PK281_08095"
     CDS             complement(2353..2805)
                     /locus_tag="PK281_08095"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOY49346.1"
                     /translation="MLGQGLIAFVQGSLVSVGFWIFDFNDPVFWGIISFFLSFLPVIG
                     APIVFVPAGLIAIANGQASDGYGIILWGFILVTNIDNVLRFFISKYMADTHPLVAIVG
                     VIIGIPIFGILGLVFGPLLISWFFLLIKIYEEGKDEEESDEEDESILA"
     gene            complement(2760..3392)
                     /locus_tag="PK281_08100"
     CDS             complement(2760..3392)
                     /locus_tag="PK281_08100"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOY49347.1"
                     /translation="MPIQDYRLRRRIILLLTFISGAFLLYALHELIPAFLGSVVLYVL
                     FRPFFGYLLYIKKWPNWLATWTIMLLTFLLIVLPLTIVGIMIGSKVSNLLQHTEAITS
                     ILGKVQNYFGVSLNDGATVEKILGFLQDNLFGGVSSFVNGVFGIIFTLGMMYFILYFM
                     LSSHRAFEKTLIKYMPFKKENTLRFAAELRNSTYSMCSDKDLLPSYKEVW"
     gene            complement(3397..4461)
                     /locus_tag="PK281_08105"
     CDS             complement(3397..4461)
                     /locus_tag="PK281_08105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49348.1"
                     /translation="MKQVTLLIAFIFQLGIQHIQAQQELAVLTYSVPEKIEAGTPFQV
                     SMTLNRGAITGIARIQQSWPRGFTINEVENAGAIFSFSKKQLQFLWVTLPTEQEIKVT
                     FEVTASKDYAGKLEIPGILAYLEGSQRKELTFNPLKLDILGKGTKAFEKPASPSLSAS
                     SDTKIKSPAIAEKPSTPVKTSPASSPVKKEVAPLKPAPKLEQKEKPVTPKKESVPIVK
                     KEVPKTPETKKGAPITEEKPKTEPVKTPAKIETTTSPSTSTSTSKSAISFRIQVAALP
                     QKADKETIARDFSVEAKDLQEESHNGLFKYTFGEYPNLAEARKKMNANPKMKGKAFIT
                     GYRNGIRIDLEEAIRLSKEK"
     gene            complement(4559..5371)
                     /locus_tag="PK281_08110"
     CDS             complement(4559..5371)
                     /locus_tag="PK281_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49349.1"
                     /translation="MIRKLLAPLALFVVISTQTFAAESIVRIAQSLSQQKAYAGGTFL
                     VKIKIERRNLNSFFQIEQELPKGMTAIGVESQGATFSCKDGKVKYNWLRLPAEEEIQV
                     MYKVTVPFEMRGKQSIEGRYYYIVDEEKEIFNLPKNTIEVIEYIAPSDSLAEKTLLGM
                     INNDQDKPVYMSEEKVDLEYKVQILSSTRALDRDSIRKEYAIKDKLQEENFNGLYKYT
                     VGSFGTYEQARDYKNKLDFSRYIPFVIAYNRGTRITVGEAMNLASKKKSVQK"
     gene            complement(5576..>6157)
                     /locus_tag="PK281_08115"
     CDS             complement(5576..>6157)
                     /locus_tag="PK281_08115"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOY49350.1"
                     /translation="PEDKKKFLINELGKRVDTTMVLMDNLLMWSRTQLNGIVFQPEIL
                     DPARLIKGVIKQLEAQADEKKISIKLQYHETNMVLVDSNMFELIIRNLISNAIKFTNP
                     EGFVKILIEKNQQKVLIRVSDSGVGMTAEMLEKLHNKEFVSTPGTKKEKGTGLGLVLC
                     QEFVGKHQGEMLIESQPQLGTVISLFFDSVEEF"
BASE COUNT         1758 a         1214 c         1263 g         1922 t
ORIGIN      
        1 tcgggatgaa atggatgatg ggttggatgc atgatacact tcgttatttc aaaaccgatc
       61 cgctctaccg cgaacatcat caaaacgatg taacattctc gatttattac gccttcaccg
      121 agagtttcat gctacctctt tcacacgatg aagtggttca cggaaaaggt tctctaatcg
      181 atcgtatgcc tggcgaccaa tggcagaaat ttgccaatct gcgagctatg tattcctaca
      241 tgttcacgca tccgggaagt aaattgcttt tcatgggcga tgaatttgga caatacagtg
      301 aatggaatta taaaactggc ctcgattggc atctcacagg ttttgagccc aatagcggta
      361 tgcaaaaact gataaaagac ctgaatacat tatatcgtaa agaaccagct cttcatagct
      421 tgcaattcga tacaaaaggg tttgaatgga tcgatttaaa cgatagtcgc aacagtactt
      481 tatctttcat tcgcagaggt gaaaagccgg aagattattt ggtgattgcc tgcaatttca
      541 caccaattcc tcaagaaaat taccgcattg gagttcccga tggtgggaaa tattcggtca
      601 tctttaatag cgataataag tcttactggg gaagtgatta ttctgcaaaa cacaagtacg
      661 aatcaaccga tgtagagaaa catggtcgtc cacattctat cgcaattgat ctgcctccct
      721 tgtcggtaca ggtgtttcgc ttgactgcac gtcataaaaa agccgctgtg aagaaaaaag
      781 cataaagggc ctttattccg atgcttgcct gtccgtaatt tgtcgtttta gcaaaccatg
      841 aaacaggaat tcatcaccgc cgttcaatcg tcatatcccg atagtcaagc acacattttt
      901 ttaggatgtg gcgtgctcaa aggagaaatt ttatccgatg ctcgggtgaa cattcctttg
      961 agtatgatga atcgccatgg tttgatagca ggagctactg gttcgggtaa aacacgtaca
     1021 cttcaggttt tagcggagca gttgagcaaa gcaggcgttc ctgtatttct ttcggatatc
     1081 aaaggtgatt tgagtggatt aggaaaaccg ggagtttcta atgctaagat tgaagaacgt
     1141 gcttcagcct taaacataaa ctttcaaccc gaaggtgttc caattgagtt gtattctctc
     1201 agcgggaaat taggtgctca aatgcgggct accgtgatgg aatttggtcc tgtcttgctc
     1261 gccaaaatcc tcgaactcaa cgaaactcaa acaggtgttt tatctatcct gttcaagtat
     1321 gccgacgata aaggacttcc cattgtggat ttacaggatt tgaagaaagt ccttaattac
     1381 ctaagcgaag gacctggaaa agcagagatt aaagaaagct acggaattat ttcaagtgcc
     1441 acttccggaa ccatcctgcg taaaattgtt gcattagagc agcaaggtgt agcggctgta
     1501 tttggggagc catcgtttga tatttccgat ttaatgttga aggttgatgg acaaggtgtg
     1561 gtgagtttgc tcaatgtaag cgatatgcaa gataaacctg ccgtgttttc tacattcctg
     1621 ttgagtttac tggtcgagtt atatgaaact ttacccgaag caggcgactt ggacaaaccc
     1681 aaacttgttt tctttctcga tgaagcgcat ttgttattta gcgacgctcc caaagcattt
     1741 ttggaacaag tagaacagat tgttcgcttg atacgctcga aaggagtagg tgtgttcttt
     1801 tgttctcaaa tggcgcagga tattccggtg aatgtattag cacaattggg gaatcgcttt
     1861 cagcatgtgt tacgtgcttt cacgccaaac gatgctaagg cattgaaaga taccgtgaaa
     1921 acctatccga gatcggcttt ttacgaaatc gaagaaacgc tcacccaaat gggaattggg
     1981 caggcattgg taacggtgct caactcgaaa ggaattccta ccgaagtggt tcatacgcat
     2041 ctcgttccac cagctgctat tatgggaccg cttcctgcaa gcgaatatca ggatcacctc
     2101 aatcagtcgg aattattcgc caagtacgcc gaaacaaccg atccggtgag tgcatacgaa
     2161 atgctcaatt cgcgattgca aaagcagcaa gaagaaacat caactacttc atcaacgccc
     2221 ccaaaatccg gaaagcaaga aaaaacgatg ttggaagaag tgatgaattc ttcggttaca
     2281 cgcagtattg ggagagaatt agtgcgtgga atttttgggg tattattcgg aagttcgaaa
     2341 cgtcgccgct gattaagcca gaatactttc atcttcttca tcggactctt cctcatcttt
     2401 cccttcttca taaatcttaa tcaacaagaa gaaccaagaa atcaacagtg ggccgaacac
     2461 taagcctaga attccaaaaa ttggtatccc gataatcact ccaacgatgg caactaatgg
     2521 atgagtatct gccatgtatt tggaaatgaa gaagcgcaaa acgttgtcga tattagttac
     2581 gagaataaat ccccacagga tgattccata accatcggat gcttgtccgt ttgcaatggc
     2641 gattaaccca gctggaacaa acacgatagg tgctccaatt actggaagga aggaaaggaa
     2701 gaaggaaatt atcccccaga aaacgggatc attaaaatcg aaaatccaga atccaacact
     2761 caccaaactt ccttgtacga aggcaataag tccttgtccg agcacattga ataagttgaa
     2821 ttcctcaatt cggcagcaaa tcggagtgtg ttttcctttt tgaaaggcat gtatttgatg
     2881 agcgtttttt caaatgcacg gtgactgctc aacatgaaat acaggataaa atacatcata
     2941 cccaaggtga aaattattcc aaacaccccg ttgacaaaag aacttacacc gccaaacaaa
     3001 ttgtcttgta aaaagccaag aatcttttct acagttgcac catcattcaa tgaaacgcca
     3061 aagtaattct gaacttttcc gaggatagaa gtaatagctt cggtatgctg gagtaagttc
     3121 gaaactttac tgccaatcat aatgcccacg atggttaatg gtaaaacgat aaggagaaaa
     3181 gtgagcagca tgattgtcca ggtagctaac caattaggcc atttcttgat gtataagaga
     3241 tagccaaaaa atgggcggaa tagcacatac agaactactg aacctaagaa tgcaggaatt
     3301 aattcatgca aagcataaag taaaaacgca cctgaaatga atgtcaggag taatatgatt
     3361 ctcctacgta ggcgataatc ttgtatgggc acaggcttat ttttctttac ttaatcggat
     3421 agcttcttct aaatcgatac ggattccatt ccgatagcca gtaataaatg ctttcccttt
     3481 cattttgggg ttagcattca tcttttttcg agcttctgcc aaattgggat attcaccaaa
     3541 tgtgtatttg aataaaccat tgtgactttc ttcctgtaaa tctttggctt ccacactaaa
     3601 atcgcgagca atcgtttctt tatcggcttt ttgaggcaat gctgcaactt gaattcgaaa
     3661 tgaaatagcc gatttgctcg ttgaagtact tgtgctcgga ctggtagtcg tttcgatttt
     3721 agcaggagtt ttaaccggtt ccgttttagg tttttcttct gtaataggag cgcctttttt
     3781 cgtttctgga gttttaggta cttccttttt tacgataggt acactttcct ttttcggtgt
     3841 aaccggtttt tctttttgtt ccagtttagg cgcaggtttt aatggcgcaa cttccttttt
     3901 tactgggctt gatgctggtg aagtttttac tggagtgctt ggtttttcag caatagcggg
     3961 tgattttatt ttggtatcgc tggaagcaga tagggaagga gaagctggtt tttcgaatgc
     4021 ttttgtgccc ttaccgagga tgtcgagttt tagtggattg aaagttaatt ccttccgttg
     4081 actgccctcg agataggcta gaataccggg tatttctaat ttgccagcat aatccttact
     4141 agctgtaacc tcgaatgtga ctttaatttc ttgttctgtt ggtaaagtta cccagaggaa
     4201 ttggagttgt tttttgctga aggagaatat ggcacctgcg ttttcaactt cgttaatcgt
     4261 aaatccacgt ggccacgatt gttgaatacg tgcaattcca gtaattgctc cacgatttaa
     4321 tgtcattgaa acttggaaag gagttcctgc ttcaatcttt tcgggaacag aataggttag
     4381 aacagctagc tcttgttgag cctgaatatg ctgaattcct aattgaaaga taaatgcaat
     4441 cagtaacgtg acttgcttca tgccacgaat atcgggaatt taggctgctg ttggtattaa
     4501 aaaacgccct gcagttttca ctacagggcg ttaggaggat ttaacacagc acagtatctt
     4561 atttctgtac cgattttttc ttcgaagcca agttcattgc ttctcccact gtgatgcgag
     4621 ttccgcggtt ataggctatt acaaaaggaa tgtatcggga gaaatcgagt ttgtttttgt
     4681 agtcacgagc ttgttcgtat gttccaaatg agccaaccgt gtatttgtac aagccattga
     4741 agttttcttc ttgcaacttg tcttttattg catattcctt acgaatagaa tcacgatcaa
     4801 gtgcgcgagt agagcttaga atttggactt tatattccaa atcaactttc tcttccgaca
     4861 tgtaaacggg cttatcttgg tcattattaa tcattcctaa taatgttttt tcagccagtg
     4921 aatcacttgg tgcgatgtac tcaattactt caatcgtgtt tttaggaagg ttgaagattt
     4981 ctttttcttc atcaacaatg tagtagtacc taccttcaat cgattgcttt ccacgcattt
     5041 cgaaaggaac agttactttg tacatcactt gaatttcttc ttcagcgggt aaacgtaacc
     5101 agttgtattt aacctttcca tctttacaac taaatgttgc tccttgactt tctactccaa
     5161 tggcagtcat tcctttagga agttcctgtt cgatttggaa gaagctgttg aggtttcggc
     5221 gttcaatttt aatcttaact aaaaaagttc cgcctgcata agccttctgt tgagaaagag
     5281 attgagcaat gcggacaata ctttcggcag caaatgtttg agttgaaata acaacaaaca
     5341 aggcaagtgg tgctaatagt ttccgaatca tgtttttttt tgttaaagtt cgtgtgttga
     5401 gttgttcatt ttaattgagt cacatcaact ttttaacagt aaaatgctgc ccaattctga
     5461 ataactgtta ctcgaataac gtttcgattg ttgcttctat tttctccatt tccttacatg
     5521 attttttttc gttttgaagg aaaatggaat tctatttcta agttttaatt agaagctaaa
     5581 attcctctac cgagtcgaaa aacaaggaaa ttactgtacc taattgtggc tgagattcaa
     5641 tgagcatttc accttgatgc ttcccaacga actcttgaca aagcaccaaa cctaatccag
     5701 ttcccttttc ttttttggtc ccaggagtgg atacaaattc cttattgtga agtttttcca
     5761 acatttccgc tgtcatgcca acgcctgaat cggatactct aatcagaact ttctgctgat
     5821 tcttttcaat caatatttta acaaaccctt ctggattcgt aaattttatt gcattggaaa
     5881 tcagattccg gatgattaat tcaaacatat ttgaatccac taaaaccata ttggtttcgt
     5941 gatactgaag ctttatgctt atcttttttt catcggcttg agcttcaagt tgttttatta
     6001 cgcctttaat taatcgtgca ggatctagaa tttcgggttg aaaaacaatt ccatttaatt
     6061 gggtacgtga ccacatgagt aaattatcca ttaataccat ggtcgtatct acccgtttcc
     6121 ctaattcatt tattagaaac ttctttttgt cttctgg
//
LOCUS       DAOOQW010000136         6148 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_15565_length_6148_cov_4.552273, whole genome shotgun sequence.
ACCESSION   DAOOQW010000136 DAOOQW010000000
VERSION     DAOOQW010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 6148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6148
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15565_length_6148_cov_4.552273"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1877
                     /locus_tag="PK281_08120"
     CDS             <1..1877
                     /locus_tag="PK281_08120"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOY49351.1"
                     /translation="FDFFHPQTFVMNKQFPGDFLEPGTTSITAVDFPIHEYIIAAGMK
                     LAQTRDPWVFRCYELLLSFLGLFYFYRLSRLVDNNKLSSLFLTGVVATAPLFTYYQAG
                     FLPGIPSLTISIVGVYYYFKHRKTNKPGTFILSIALFTLAAATRTTFVIPLIAIASME
                     FLRMIQERKFFWKHWGTLFLGFGFLASYFIYNNHLRAEYGSIFLNKLKPAASFGEAID
                     IVATVFDTWIYHYFNPIQIVLLAFLITIAILQFLKKRGEFESLSRKLLQFTLISLLGC
                     LFFLFAMLQQFRVHDYYFLDTFFLPGILFFLLLFRYLKSSIESINYSIYLAAGLLVLM
                     VYLAASIQEEKRGTGHWDKIASMVRDYKGLDVKLDSMGISRSATLLVLDSQAPNIPFI
                     RMQRKGFAVVTTSEANLKNALNWKYDYILLQDEWFLSDIFPNYPEIINYMHKVADLGP
                     VSIFKHIEFEKERTLADFYRLNEKPALLQVNTDFETTLESPWEHTETDSTQSFEGKFA
                     GITSPDNEFGITYRSPVTSIFNGKETMLIVSAAFLNPQLAPESRLVVAIDEGGKNVFY
                     KDTPIETLKRERQAWKKSLSLFMIPACGTNCKLSVYIWNRGKNTIHIDNLDISVYSNN
                     "
     gene            2086..2472
                     /locus_tag="PK281_08125"
     CDS             2086..2472
                     /locus_tag="PK281_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49352.1"
                     /translation="MFRFSLLSVLSLFTLISCSKEPGEGGSATISGKVFGYDVNTSGV
                     ITDSAYAGDYRVYISYGDNSAADNDVRTSYSGDYTFKGLRKGKYKVYVFSQCDTCVFN
                     QNYIVQDVEITSNKQDLTLPDFVIYD"
     gene            2480..3256
                     /locus_tag="PK281_08130"
     CDS             2480..3256
                     /locus_tag="PK281_08130"
                     /inference="COORDINATES: protein motif:HMM:NF020916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HOY49353.1"
                     /translation="MSSEHPLLAILDQFQAASLDDMNRLKLLNRVDQKFIFRYEELES
                     MLKEVLNDYNILAIDDKRLFQYNTLYFDTTAYSSYTDHHNGKPNRYKIRYRKYQDSGD
                     VFFEIKKKIKGLRTDKHRVPTENIPQALGNAEKELLSECVKDGLQLVPTMWVNYKRLT
                     LMSRETEERVTIDLNLHLKKETEEVSLEGLVIVEVKQAKASRNSPFLECLREKQIRET
                     RISKYTIAVALLNHEIKRNAFGSKLRKIKHLIEDQWKQAS"
     gene            3238..3891
                     /locus_tag="PK281_08135"
     CDS             3238..3891
                     /locus_tag="PK281_08135"
                     /inference="COORDINATES: protein motif:HMM:NF027640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HOY49354.1"
                     /translation="METSELVNEIAATHGWLEFITRFGINFFVIFVLIRILYYPMSKN
                     RDFLFTFFLFNIINFLICFLLSSAKLKMGFAFGLFAIFSILRYRTVTVPVREMGYFFV
                     SVTLGIINSLTGIDDNYLLLGTANAIILLTAWIIDYQVKIVHENSKEIIYDRIDLIHP
                     DKQEEMMEDIRKRTGLNVHRVEMVRIDFFKDVARMFVFYNSARNDTPSRGGSASDED"
     gene            3881..4570
                     /locus_tag="PK281_08140"
     CDS             3881..4570
                     /locus_tag="PK281_08140"
                     /inference="COORDINATES: protein motif:HMM:NF022142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2490 domain-containing protein"
                     /protein_id="HOY49355.1"
                     /translation="MKINPYLFLILLLSTCKLSAQSRDFQVWNALALNQDLKKGFDLS
                     AQYQSRIDKDISRFRGSYMSVSLGYKLNKNWTTEAGIRYSTSNRWDRIRYSVGIGRSW
                     KWDKLEISAKAKYQYQYYLESLPEWGINPPAQNFRLKLGAEQKIIKKLKVFVSTEPMF
                     RYKSSEFYFRRLRNTAGIKWSPIKRTSFEASYIWQPQWNSDAVIQVLQFNVSYDLPKM
                     GKKKVKEKASK"
     gene            4615..5856
                     /locus_tag="PK281_08145"
     CDS             4615..5856
                     /locus_tag="PK281_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TCR/Tet family MFS transporter"
                     /protein_id="HOY49356.1"
                     /translation="MSNSRKPAIGFIFATLLIDITGLGIIIPVFPKLIQELTGGTLSD
                     AAEYGGWLLFSFAIMQFLFSPVLGNLSDQYGRRPILLTSLFGFALDYLFLAFAPSIFW
                     LFVGRLIAGIMGASITTATAYIADISTPEKRAQNFGLVGAAFGLGFIIGPVVGGILGE
                     YGSRIPFMAAAALTFINFLYGYFILPESLSIENRRKFDWKRANPIGALRHLQKYPIIS
                     GLVLSLLFVYIASHAVQSTWSFFTMERFGWSEKAVGYSLGFVGILVAIVQGGLIRYIN
                     PWLGQKRSVYWGLILYCIGLSCFAFANQSWMMYAALIPYCLSGICGPALQGIMSAQVP
                     GSEQGELQGALTSLVSLTSIIGPVMMTNVFARFTADNAPFYFPGAAFMLGAIFCFLSL
                     FFALRTLGKHSELIAANEKRS"
BASE COUNT         1796 a         1187 c         1158 g         2007 t
ORIGIN      
        1 gtttcgattt ctttcatccg caaacttttg tgatgaacaa acagtttccc ggtgactttt
       61 tagagccggg aacaacaagt attacagccg tcgattttcc cattcatgaa tacattattg
      121 cggccggaat gaaactcgct caaacgcgag atccatgggt atttcggtgt tatgaattac
      181 tactgagttt cttggggtta ttctatttct atcgattatc ccgtttggta gataataaca
      241 aactcagttc tttatttcta accggagtgg ttgcaactgc acctttattt acttattacc
      301 aagctggctt tttaccgggc attccttccc taaccattag tattgttgga gtttactatt
      361 acttcaagca tagaaaaacc aacaaaccgg gaacatttat tttgtctata gccttattta
      421 cgttagctgc tgcaacgcgt acaacttttg tcattccgct tattgccatt gcttcgatgg
      481 agtttttacg gatgattcaa gaacgaaagt ttttctggaa acactggggc actttattcc
      541 ttggttttgg atttctagct tcctatttca tttataacaa tcatttacgt gcagaatatg
      601 gctctatctt cctgaataaa ctcaagccag ctgcaagttt cggagaagca attgatattg
      661 tagctaccgt tttcgatacc tggatttatc attatttcaa ccccattcaa atagtacttc
      721 tagcatttct gattacgatt gccatcttac agtttttgaa aaagcgaggc gaattcgaat
      781 ccctttctag aaagctactt caatttactc tcattagttt gctaggttgc ttgttcttcc
      841 tgtttgctat gttgcaacag tttcgagttc acgactatta ctttctcgac acgttctttt
      901 tacccggaat tctctttttc ctccttctat ttcgttacct caaatcttct attgaaagca
      961 tcaattattc aatttatcta gcagccggac tcttagtatt aatggtctat ttagctgcct
     1021 ccattcaaga agaaaagcgt ggcactggac attgggataa gattgcttct atggttagag
     1081 attacaaagg cttagatgtg aaactcgatt cgatgggaat ttcacgttct gctactttat
     1141 tagttttaga ttctcaagca cctaatattc cttttattcg catgcagcgt aaaggatttg
     1201 ccgttgtaac tacttcggaa gcaaatctca agaatgcctt aaactggaaa tacgattata
     1261 tattacttca agatgagtgg tttttgagtg atatctttcc caactatccc gaaatcatta
     1321 actacatgca taaagttgct gatttgggac cggtaagtat ttttaaacat atcgagttcg
     1381 aaaaagagcg aacattggct gatttttatc gtcttaacga aaaaccagct ctcctacaag
     1441 tcaataccga ctttgaaaca acgctagaat ctccatggga acatacagaa accgattcaa
     1501 cacaatcgtt cgaaggtaaa ttcgctggaa tcacttcacc tgacaacgaa tttggaatta
     1561 cctaccgctc tcctgtaact tccatcttta atggaaagga aacaatgcta atcgttagcg
     1621 ctgcttttct gaatcctcaa ttagctcctg aaagtcgttt agtagttgct attgacgaag
     1681 gtggtaaaaa tgtattttac aaagatacgc ctatcgaaac actaaaaaga gaacgccagg
     1741 catggaaaaa atcactttct cttttcatga ttccagcttg tggaacgaat tgcaaactat
     1801 cagtatatat ctggaataga ggcaaaaaca ccattcatat cgacaaccta gatatttcgg
     1861 tttattccaa caactaataa ataatgatgg aacattaaac taatgttaag ttattagtct
     1921 taaggtcgca ttttcttttt ttaaataata ctactgttgg ctaattagct tttaactaag
     1981 ctttttataa aacaatactg attttaaaag tgttattttc attctcaact acaggaataa
     2041 acaagattga attaactttt gcgtaatatt cgcccaaaaa ataccatgtt tcgattttca
     2101 cttctgtccg ttttaagttt attcaccttg atttcctgca gcaaagaacc tggagaaggt
     2161 ggttctgcta ctattagcgg gaaagttttt ggatatgatg tgaatacaag tggagtgatt
     2221 accgatagtg cttatgcagg tgattatcgc gtttatatat cctatggaga taatagcgcc
     2281 gctgataatg atgttcgaac ttcttattcg ggcgattata cattcaaagg tcttcgaaaa
     2341 ggaaaataca aggtgtatgt gttttcacaa tgcgacacgt gtgtattcaa tcaaaattat
     2401 atagttcagg atgtggaaat cacttcgaat aaacaagatt tgacacttcc cgattttgtt
     2461 atctacgatt gatacaagca tgagttctga acacccgctt ttagctattc ttgatcagtt
     2521 ccaagctgct agtctagatg atatgaaccg acttaaacta ctcaatcgag tagatcaaaa
     2581 gttcattttc agatacgagg agcttgaatc gatgttgaaa gaagtgctca acgattacaa
     2641 cattcttgca atcgacgata agcgattgtt tcaatacaat accttgtatt tcgatacaac
     2701 tgcctattca tcctataccg atcatcacaa tggaaaaccc aatcggtata aaatacgcta
     2761 tagaaaatac caagattctg gggatgtgtt tttcgaaatc aagaagaaaa ttaaaggatt
     2821 aagaactgat aaacatcgtg taccaactga gaatatcccg caagcacttg gaaatgctga
     2881 aaaagaatta ctgagtgaat gtgtgaaaga tggattacag ttagttccta ccatgtgggt
     2941 taattacaaa cgattaacgc tcatgagtcg cgaaacagaa gagcgagtta ccatcgacct
     3001 caacctacac ctcaaaaaag aaacagaaga agtttcactc gaaggcttag tgatagttga
     3061 agtgaagcaa gctaaggcat cacgaaatag tccgtttttg gaatgtttgc gtgagaaaca
     3121 aatacgagaa acccgcataa gtaaatacac cattgcagta gcattattaa accacgaaat
     3181 aaagcgcaat gcatttggct ccaaactccg taaaataaaa cacctaattg aagatcaatg
     3241 gaaacaagcg agttagtgaa tgagatagct gcgacacacg gttggctgga gtttattacc
     3301 cgattcggga taaatttttt cgtgattttt gtccttattc ggatactcta ctatccaatg
     3361 agcaagaacc gcgatttctt gttcaccttt ttccttttta acattataaa tttcctcatt
     3421 tgcttcctac taagtagtgc gaaactcaaa atgggctttg catttggctt gtttgccatt
     3481 ttttccattc tgcgttatcg aactgttacc gttcctgttc gcgaaatggg gtatttcttc
     3541 gttagcgtga ctttgggtat tatcaattca cttactggca ttgatgataa ttatttatta
     3601 ctaggaacag caaatgccat tatcctacta acagcttgga taattgatta tcaagttaaa
     3661 atcgttcacg agaactcaaa agaaatcatt tacgaccgta tcgatttaat tcatcccgat
     3721 aaacaagaag agatgatgga agacatacgt aaacgtacgg gtttaaatgt gcatcgtgta
     3781 gaaatggttc gtatcgattt ctttaaggat gttgctcgta tgtttgtttt ctacaattct
     3841 gcacgaaacg atacaccaag ccgaggagga agtgccagcg atgaagatta atccatatct
     3901 gtttctaatt cttctcttat ccacatgtaa actctcggct caatcccgcg attttcaagt
     3961 gtggaatgcc cttgctttga atcaggattt aaagaaagga ttcgacctaa gcgcacagta
     4021 tcaatcacgt atcgataaag atatttcacg cttccgcgga agttatatga gtgtatcatt
     4081 gggctacaaa ctcaataaaa attggactac cgaagctgga atccgatata gcacatccaa
     4141 tcgatgggat cgaattagat acagtgttgg aattgggcga agttggaaat gggataaatt
     4201 ggaaatctct gcaaaagcca aataccaata ccaatattac cttgaaagcc tgccggaatg
     4261 gggcatcaat cctccagctc aaaatttcag attgaagtta ggtgcagaac aaaaaatcat
     4321 caaaaaactc aaggtttttg taagcacaga gcccatgttt agatataaat cctctgagtt
     4381 ttattttcga cgcttgagaa acacggctgg aataaaatgg tctccaatta aacgaaccag
     4441 tttcgaagca tcctacatct ggcaaccaca atggaattct gatgctgtaa ttcaggtact
     4501 tcaattcaat gtttcctacg atttacctaa aatgggtaaa aagaaagtta aggaaaaagc
     4561 atcgaagtag ttaagtgggg gaattcagta catttgaaaa cctgacccaa tcacatgtca
     4621 aactcccgta agccagccat tggctttatc tttgcgactt tattaatcga tattactgga
     4681 ttaggaatta tcattcctgt atttccgaaa ctcattcaag aacttactgg tggaacactt
     4741 agcgatgctg ctgaatacgg tggatggttg ctgttttcgt tcgccattat gcagttttta
     4801 ttttcgcccg ttttgggaaa tctaagcgat caatatggca gaagaccaat cttactcacc
     4861 tcgctttttg gttttgcgct cgactacctg tttttagctt ttgctcctag cattttctgg
     4921 ttattcgtag gccgactcat agctggaatt atgggtgcga gtattaccac agctacagcc
     4981 tacatcgccg atatttcaac tcccgaaaaa cgtgcacaga attttggtct agttggagct
     5041 gcatttgggt taggatttat tattggtcct gttgtaggtg gaattttagg cgaatatggt
     5101 tcccgcattc cttttatggc tgcagccgct cttacattca tcaatttcct gtacggatat
     5161 tttattcttc ccgaatcttt atccatagaa aaccgtcgca agttcgattg gaaacgtgct
     5221 aatcccattg gtgcacttcg tcacttacag aaatatccaa tcatctctgg cttagtttta
     5281 tcgctgcttt tcgtgtatat cgcatcacat gccgtacaaa gcacttggtc gttttttacc
     5341 atggagcgct ttggctggtc cgaaaaagcg gttggttatt ctcttggctt tgttggaatt
     5401 ctggtggcaa ttgtacaagg tggattaata cgctatatca atccatggct gggacaaaaa
     5461 cgctcagttt attggggttt gattctctat tgtatcggtt tgagctgttt tgcttttgcc
     5521 aatcaaagtt ggatgatgta tgctgcgttg attccttatt gcctatcggg aatttgcggt
     5581 ccagctttac aaggaattat gtcggcccaa gttcccggct ccgaacaagg cgaattacaa
     5641 ggtgctttaa ctagtttagt gagcctaact tcgattattg gtccagtaat gatgaccaac
     5701 gtatttgccc gatttactgc cgataatgca cctttttatt tccccggtgc ggcctttatg
     5761 ttaggagcta tattttgctt tctgagtttg ttttttgccc taagaacact gggaaaacac
     5821 tctgaactga ttgctgcaaa cgaaaagcgt agctgatttc ttagcagcta agagttatat
     5881 atttcgatgt aattcttgct tgcccaaatt tcctggttaa attttcttga ttcggattga
     5941 gatggtgcat tcgattcaat ttaaaagata tttaggtctg ctttttattt gttttcctat
     6001 cttttttatt tcattgattt ctttatcagt attttaagca agtattcatt ttacctaaaa
     6061 atttgaaaag gctctgcttt acctatttga aattcagaaa catgccgata tgatagttta
     6121 acttaatttt cggcttagcc ccgaaggg
//
LOCUS       DAOOQW010000137         6116 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_15651_length_6116_cov_4.446626, whole genome shotgun sequence.
ACCESSION   DAOOQW010000137 DAOOQW010000000
VERSION     DAOOQW010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 6116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6116
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15651_length_6116_cov_4.446626"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2235
                     /locus_tag="PK281_08150"
     CDS             <1..2235
                     /locus_tag="PK281_08150"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY49357.1"
                     /translation="DLLSGERDFLVELDTLNTFDSPLKKQFTVKGNVLGRQAVDLLSL
                     DSLAYYWRTKLINPLAGESEAWTESSFTYINNGSEGWAQVHFPQYIENQGEGLEKDAT
                     LRRLNFKETITAIDIKTFGGSAGKPNDSVSVKIGGAEYNLYTQSGGGFGCRNNTINLI
                     AFDKKSTVPYVGVYFKWYEILYTYGGRRLVCGREPFVINSFTPSELSTGNNDDIIKYV
                     DNILVGDSVVLYSMGDAGYSAWPAAAKNKLGELGISVAQIDDLLPGEPVVIFARKGLA
                     PGSATILKTSGPQPEQQKLVVNKTITGRYLSGEMKSTLIGPAQQWQSFIGQPSEIEAS
                     DIAYFDIHGITLDGDDQLLFSHVSYGMDLSSVSADDYPYLKIVFYAEDNINLTAAQLK
                     KWLVTYTPVPEGLLIYNGITSQEIISEGEEWKSTYGFMNISETTFADSLTVRYEVFNQ
                     STRNSNLEVFKIKQPAPGDTTLFDVTFNSSNQTGLNDVNVFVNQLVLPELYYDNNVIE
                     LREHLNVQIDNFDPVMDVTIDGRRVLNNDFVFSNPFILVKVWDENRNSLITDISTVQI
                     FLTYPCDVENCPVTQIDLSSDDVKWYPATNESDFRIEFKPTNLPDGKYTLRVLATDAK
                     GNESGAIPYLVDFIVKNETTVVISDPYPNPFAARTYLNITVSGNTLPDQYQFEIFDLN
                     GKLLREFQQDEMPPIFIGTNIISWSGTDDYGNEMVNGMYLFKLSLSIDGNQIEKRGKI
                     VLVR"
     gene            complement(2236..3279)
                     /locus_tag="PK281_08155"
     CDS             complement(2236..3279)
                     /locus_tag="PK281_08155"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF008277.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HOY49358.1"
                     /translation="MDSSHTTSSRLNVFTLKNKNGLSMTITNFGGKVMSMLIPDRNGK
                     LNDIILGYGTPEEYINGSPYFGALIGRVGNRIAKGKFSLNGNTHQLPINNGENHLHGG
                     PNGFHNVYWDATPLSINGNEALQLEYTSKNGEQGYPGNLKVIVKYILTNENDWIVEYE
                     ATTDQSTPINLTQHNYYNLAGEGNGDILNHRLAIHADHFTPVDSSLIPTGELRSVANT
                     PFDFRKPISIGEHINDDDEQLKFGSGYDHNWVLVKKDNSPTLAAVVSEPTSGRIMEIY
                     TTEPGIQLYAGNFLDSTDIGKSKIAYAHRTGFCLETQHFPDSPNRLDFPSVILKPGEK
                     YYQKTIHRFMTDK"
     gene            complement(3394..3768)
                     /locus_tag="PK281_08160"
     CDS             complement(3394..3768)
                     /locus_tag="PK281_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014782174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HOY49359.1"
                     /translation="MNARNTILTGSTFGLLGVAIGAFGAHALKPMLIESGRLETFELA
                     VRYQFYHTFALLATGILMNQLPIQFLKFASWFFTGGILFFSGSLYLLCFSGLGVLGAI
                     TPIGGLLFIAGWICIFMSALKK"
     gene            complement(3791..4453)
                     /locus_tag="PK281_08165"
     CDS             complement(3791..4453)
                     /locus_tag="PK281_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013552938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HOY49360.1"
                     /translation="MNINIKITNLKQTLPPDCKLIAVSKTHPIERLAEAYTAGQRVFG
                     ENKVQEIVPKYEALPKDIEWHMIGHLQSNKVKYIAPFIHLIHSVDSTRLLEEINKQAG
                     KLERTIPCLLQLHIAKEETKFGFSEDEVMELLQSTSFKNYQNIKIVGLMGMATFSDNP
                     GQVRNEFRSLKLFFDRLKKTSLPTNVDIKELSMGMSGDYKIAIEEGSTMVRVGSAIFG
                     SR"
     gene            4497..6053
                     /locus_tag="PK281_08170"
     CDS             4497..6053
                     /locus_tag="PK281_08170"
                     /inference="COORDINATES: protein motif:HMM:NF015291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HOY49361.1"
                     /translation="MAILGTLLKKGIRIREMLEQEYTSPFDLQKKELKELIITASQTE
                     FGKRYDFQDVLQRFKKSDKSFYKLYKQNVPIHTYNKIYSDWWKLSLKGVKNICWPGRT
                     KYFALSSGTSEAASKYIPITKEMTKAIQKTSIRQMLTLSKYDLEPEIFQSGILMLGGS
                     THLNKRGSYFEGDLTGIQAARLPFWFQHFYKPGKKIAKNRNWDVKLDEIAKKASDWDI
                     GILVGVPAWIQILIEKILKYNNAKNIHEVWPNLKVYVHGGVSFDPYRKGLEKLFGKPV
                     YYIETYLASEGFFAFQAYPNRRSMRLVLNNGIFYEFVPFEEKNFDANGEIKPDAETLM
                     IDEVEEGKEYAILISSCAGAWRYIIGDTIKFTSLEESEIIITGRTKHFLSLCGEHLSV
                     DNMSKAIELASNDFNIRIPEFAVAGVPHGTLFAHQWFVGTDDKVDADALKLKIDAHLK
                     VLNDDYAVERSAALKDIYVKVLPVKTFYDWMESKGKKGGQNKFPRVMKNAQLEDWKEF
                     LQLHANGNHS"
BASE COUNT         1867 a         1108 c         1301 g         1840 t
ORIGIN      
        1 gacttacttt ctggcgagcg tgatttctta gtggagctcg atacgctaaa cacatttgat
       61 agtccgctta aaaaacagtt taccgtaaaa ggaaatgttc tgggcagaca ggcagtagac
      121 ttattgtccc tggatagcct tgcgtattac tggcgaacaa aactgattaa tcctcttgcg
      181 ggtgagagtg aagcgtggac ggaaagctcg ttcacctaca tcaacaacgg atcagaagga
      241 tgggcacaag ttcattttcc tcaatacata gaaaatcaag gagaggggtt ggaaaaagat
      301 gcaacgcttc gaagattgaa ttttaaagaa acgataaccg ctattgatat taaaacattt
      361 ggaggttctg ctggcaaacc caatgattcg gtaagtgtaa agattggtgg tgcagagtat
      421 aatttataca cacaatcagg tggaggtttt ggctgcagaa acaatacaat caatttgatt
      481 gccttcgata agaagtcgac cgtaccgtat gttggtgtct attttaagtg gtatgaaata
      541 ttgtatacct atggcggaag aagattggtg tgtggtcgtg aaccatttgt aataaatagc
      601 tttacaccct ctgaattgag cacaggaaat aatgatgaca tcataaaata tgttgacaat
      661 attctggttg gtgactcggt agtgttgtat agtatgggtg atgccggata tagtgcgtgg
      721 cctgctgctg caaaaaataa attaggtgag ttaggaattt cggtggcgca aattgatgat
      781 cttttaccag gtgagccagt tgtgattttt gcccgcaagg gattggcccc tggctcggca
      841 acgattttga aaacttctgg tccacaaccg gaacaacaaa agcttgtagt gaataaaacc
      901 atcacaggtc gatacctttc aggtgaaatg aagagcacgt tgatcggccc agctcaacaa
      961 tggcaatcgt ttattggaca gccttcggag attgaagcat ctgacattgc ttattttgat
     1021 attcatggga taacgttaga tggtgatgac caacttctat tttctcatgt cagctatgga
     1081 atggatttaa gttcggttag cgctgatgac tatccgtatc tgaaaattgt attctatgct
     1141 gaagacaata ttaatcttac cgctgcacaa ttaaagaagt ggctcgttac ctatacacct
     1201 gttcctgagg gactattgat ttacaatgga atcacatcac aggaaataat tagtgaaggt
     1261 gaagaatgga agagcacata tgggtttatg aacatcagtg aaacaacgtt tgctgattca
     1321 cttactgttc gatatgaagt cttcaatcaa agtactcgta attcaaatct tgaggttttt
     1381 aaaattaagc aacctgctcc gggagataca acactttttg atgtaacatt caattcgtca
     1441 aatcaaacag gcttgaacga tgtaaatgtt tttgtcaatc aacttgttct acctgaattg
     1501 tattacgaca ataatgtgat tgaattgcga gaacacttaa atgtgcagat tgataatttc
     1561 gatccggtga tggatgtgac catagatgga agacgtgtac taaacaacga ctttgttttc
     1621 tctaatccct ttattctggt taaggtatgg gacgaaaacc gaaactcttt gataaccgat
     1681 attagtaccg tgcagatttt tcttacctat ccatgcgatg tagaaaactg cccggttaca
     1741 cagattgatt tatcatccga tgatgtaaag tggtatcctg ctaccaatga atctgatttc
     1801 agaattgagt ttaaacccac taatctccct gacgggaaat acaccttgcg tgttttggct
     1861 actgatgcaa agggtaacga aagtggcgca ataccttacc ttgttgattt tatagttaag
     1921 aatgaaacaa cagttgtgat cagtgaccca tatccaaatc cgtttgctgc gaggacgtac
     1981 ctcaacatta cagtgagtgg aaatacatta cctgatcagt atcagtttga gatttttgat
     2041 ttgaatggaa agttgcttcg tgaatttcaa caagatgaaa tgccacccat ttttattgga
     2101 acaaatatca tttcgtggag tggtacggac gactatggta atgaaatggt aaatggaatg
     2161 tatcttttca aactatcact ttcgattgac ggaaatcaaa ttgaaaagag gggcaagatt
     2221 gttttagtcc gctaactatt tatcggtcat gaaacgatgt atggtttttt gataatattt
     2281 ctctcctggc tttaaaatca cagaaggaaa atccaaacgg ttcggtgaat ccggaaagtg
     2341 ttgagtctct aaacaaaacc cggtgcggtg tgcataggct attttgcttt taccgatatc
     2401 ggtgctgtct aaaaaattgc cagcatataa ttgtataccc ggttcggttg tataaatttc
     2461 cataatgcgt ccagaagttg gttctgaaac aacagcagca agtgtaggcg aattgtcttt
     2521 ttttactaac acccagttgt gatcatatcc acttccaaat ttcaattgct catcgtcatc
     2581 attaatatgt tcaccaatag atataggttt tctaaaatca aacggtgtgt ttgctacgct
     2641 gcgtaattca ccggtaggaa taaggctact atcaaccggt gtgaaatgat ctgcatgtat
     2701 tgctaaacgg tggtttaaaa tatcaccatt accttctcct gctaaattgt aatagttgtg
     2761 ttgcgttaaa ttaatcggtg tggattgatc ggtggtggct tcgtattcaa cgatccaatc
     2821 gttttcattc gtaagaatat actttacaat tacttttaag ttacccggat aaccttgctc
     2881 gccattcttg cttgtatatt ctaattgaag cgcttcgtta ccattaattg aaagaggagt
     2941 agcatcccaa tacacattat gaaaaccgtt tggtccacca tgcaaatgat tttctccatt
     3001 gtttattggc aactgatgtg tatttccatt caatgaaaat tttcctttcg caatacgatt
     3061 gccaacgcga ccaattaatg caccgaaata cggactacca tttatatatt cttccggagt
     3121 gccatagcct aaaataatgt cattaagctt tccgtttcga tcgggaatca acatggacat
     3181 tacctttcct ccgaagtttg taatggtcat ggataaaccg tttttatttt tcaatgtaaa
     3241 aacgtttagc cttgaagaag tagtatgaga tgaatccata acggaaagga gagaggtttt
     3301 atagttaaac agctggattt aaaatagcta aatattcttc acacttagta atctggtaag
     3361 actttaattg gccaggtttt gtgacagaaa ttgttatttt ttaagtgcgc tcatgaagat
     3421 acagatccaa cctgcaataa agagcagacc accaatagga gtgatggcac ccagcacacc
     3481 taagccggag aaacataata gataaagact cccactgaag aaaagtatac caccagtaaa
     3541 aaaccaggat gcaaatttca gaaattgaat aggtaattgg ttcattaaaa ttccggtagc
     3601 caataacgca aaggtatggt agaattgata gcgcactgcc aactcaaagg tttccagtcg
     3661 gcctgattca ataagcatgg gtttaagtgc atgcgctcca aaagctccga tggcaacacc
     3721 aagcaatcca aaagtagatc cggttaatat tgtatttcgt gcattcattt tgtttctttt
     3781 ttacggatgt ttaccgactg ccaaaaatag ctgaaccaac acgcaccatg gtgcttcctt
     3841 cttctattgc aattttataa tcaccactca tgcccataga gagttccttt atatcaacgt
     3901 tagtaggaag cgaagtcttc tttaagcgat cgaaaaatag ttttaaactc ctgaattcgt
     3961 ttcgaacctg tccaggattg tcgctgaaag tagccatgcc catcaatccc acaatcttta
     4021 tgttttgata atttttgaac gatgttgatt gaagcagctc cattacctca tcttctgaaa
     4081 atccgaactt ggtctcttcc tttgcaatat gaagttgcaa caggcatggt attgtacgtt
     4141 ccaattttcc ggcttgcttg tttatctcct caagcaaccg ggtgctgtca accgagtgga
     4201 tgaggtgaat aaagggtgcg atgtacttta ccttgtttga ttggaggtgt ccgatcatgt
     4261 gccattcaat atcttttggc aatgcttcat atttcggaac gatttcctga accttatttt
     4321 ccccgaaaac acgttgaccc gctgtatacg cttccgccag tctttcgatt ggatgcgttt
     4381 tgctaacggc aatcagttta cagtcaggag gtaaggtttg ctttaaattg gtaattttaa
     4441 tattgatatt cattgttatt tttgaccact ttataaaaca gcgttaacgt aaagatatgg
     4501 cgatacttgg aaccttgctt aaaaaaggaa tccgcatccg cgaaatgttg gaacaggagt
     4561 acacctctcc attcgatttg caaaaaaagg aattaaaaga gctcatcatt acggcaagcc
     4621 aaacagaatt tgggaaacgc tacgattttc aggatgtgct tcagcgattt aagaaaagtg
     4681 ataaatcttt ttataagctg tataaacaga atgtacccat ccatacctat aataaaatct
     4741 actcggattg gtggaaatta tcattgaagg gtgttaaaaa tatttgctgg ccgggacgaa
     4801 cgaaatattt tgcgctgagt tcgggaacat cggaggcagc ctctaaatat attcccatta
     4861 ccaaggaaat gacgaaggcg atccagaaaa ccagtatccg ccagatgctt accctttcaa
     4921 agtacgattt ggagcctgaa atttttcaat ccggaatatt gatgttgggc ggaagcacgc
     4981 acttaaataa gagaggaagt tattttgaag gtgaccttac cggaatacaa gctgcacgat
     5041 tgccgttttg gtttcaacat ttttataaac ccggaaagaa aattgccaag aacagaaact
     5101 gggatgttaa gctggatgag attgcaaaga aggcaagcga ttgggacatt ggtattttag
     5161 ttggtgtgcc tgcgtggata caaatactaa ttgaaaaaat actgaagtat aacaatgcaa
     5221 aaaatattca tgaagtctgg cctaacttaa aggtgtatgt acacggtggt gtttcgttcg
     5281 atccctatcg aaaagggctc gagaaattat ttggcaaacc ggtttattac attgaaacgt
     5341 atttagcttc ggaaggcttt tttgcttttc aggcatatcc aaacagacga tccatgcgat
     5401 tggtgctgaa caatggaata ttctatgagt tcgtaccctt cgaagaaaag aattttgatg
     5461 caaatggtga aatcaaaccc gatgctgaaa ctttaatgat tgatgaagtg gaagaaggaa
     5521 aggaatatgc tattctgatt tcttcctgtg ccggtgcatg gcgctacatc ataggtgata
     5581 ccataaaatt cacatcgctt gaggaatctg aaattataat aacaggtcgt accaaacatt
     5641 ttctgagttt gtgtggcgag cacctttcgg tggataacat gagcaaggcg attgagctcg
     5701 catcaaatga ttttaatatt cgaattcctg agttcgcagt ggcaggggta ccccatggaa
     5761 cactcttcgc gcatcaatgg tttgtgggca cagatgataa agtagatgca gatgcattga
     5821 agttaaagat tgatgcacac ctgaaagttt tgaacgatga ttatgccgtg gagcggagcg
     5881 ctgcactgaa ggatatttat gttaaggtat tgccggtaaa aacattttac gattggatgg
     5941 agtcgaaagg taaaaaggga ggacagaata aatttccacg agtgatgaag aatgctcaac
     6001 tagaagactg gaaagaattt cttcaactgc acgccaatgg aaatcattct taagggaatt
     6061 gtatcaggta tagtcctggc gtttttaatt ggccccgttt tttttacgct tattca
//
LOCUS       DAOOQW010000138         6108 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_15677_length_6108_cov_3.901371, whole genome shotgun sequence.
ACCESSION   DAOOQW010000138 DAOOQW010000000
VERSION     DAOOQW010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 6108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6108
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15677_length_6108_cov_3.901371"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1170
                     /locus_tag="PK281_08175"
     CDS             <1..1170
                     /locus_tag="PK281_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HOY49362.1"
                     /translation="SSMVLGVELGYDFPQVAKPLKVLSDIFLRLIKTIIAPLLFSTLV
                     VGIAGHSSLKQVGRMGLKAILYFEIVTTFALFIGLAAINFTGAGKGAVFQAQDAQVEK
                     ANSLISQKQSHDVILDIFPENIAKSIFDNQILQIVVFSILFGIGLAMVKNEDKKRPML
                     AFAESLSEVMFKFTHLVMYLAPLAVGGAVAYSVATMGLGVLQNLFMLVATLYGALGAF
                     VLLILVPIGLIVRLPFKRFLEAISEPVSIAFATASSEAALPKAMENLEAMGIPRKVVA
                     FVLPTGYSFNLDGTTLYLSLASVFVAQVCGVDLSLSEQIQMCLILMLTSKGVAGVRGA
                     SFLILVSTVASLGLDPQKAFAILAIDAVMDMGRTSVNVVGNCLATYVVARWEKEI"
     gene            1268..3547
                     /locus_tag="PK281_08180"
     CDS             1268..3547
                     /locus_tag="PK281_08180"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HOY49363.1"
                     /translation="MKKLSTLFLSTLIITSAFAQQQVHRCATMEVDNRLKQTHPEYAA
                     AREQIEEHARAFAIHGSGQRQLVTIPVVFHVVYRNATQNISDAALLSQIDILNEDFRK
                     LNADFSGTPSVFQGAAADLEIQFCLATVDPNGNATTGITRTLTTDNGFDDDDQVKYTS
                     QGGHDIWNRNKYLNLWVCDLSSAGLLGYAQFPGGPAATDGVVITYDAIGRPPANNFGG
                     AYNLGRTATHEIGHWLNLYHVWGDSNCGNDQVSDTPTQQTANYGCKTFPHVTCNNGPN
                     GDMFMNYMDYGDDDCLKMFTNGQKTRAQALFATGGSRVSLLTSNVCSSVPPVTTCSDT
                     LNYPLNGTVVTYTASATLSDGYVCGTNLYEDVAKADWFTATAPLTSLAGALFTFGAAS
                     QGTAPAGSTITVKAYNYTASGPGSVIGTASIPLSTIIANVNANQNTYVAFPSPITVTG
                     NFFLGIEFNPTVGYVLGLKSNSDGDATVNKAWEQFGDLSWHQFTENPASWGISINQAI
                     FPILTSASPVATFSPSVTSICTGQSVTYTPVAGAASYSWTFTGGSPSSSTQQNPTVTY
                     NSTGSFGASLTVTGACSGQSSSQTQNSLVTVTQSPALPVININGTELSVSITGVTYQW
                     FLNGNAISGATSSTYTPTAAGSYTVRVANGNCTKTSSAINFNPLEIETVGDIQLSVFP
                     NPANDLLSVKAVFSNQQDVVDCRLFDNSGKLVLHRTFEHIQPDALLQFGISEISSGMY
                     QLVLTTATGHSVSRVAIAR"
     gene            3634..4647
                     /locus_tag="PK281_08185"
     CDS             3634..4647
                     /locus_tag="PK281_08185"
                     /inference="COORDINATES: protein motif:HMM:NF025749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4421 family protein"
                     /protein_id="HOY49364.1"
                     /translation="MAFKAQEQPRKWTRDTNYVTSFTDQPHITFELAKRNQRIDIRNP
                     LLDTFLLRYEPNSKTNLIGSIDYRWLSLSLGLVTIQSGDSYRKGSSTQFSLRASFNGR
                     RIWNSNFIQVIKGYYLTNPLMVDPYWNPLVDQYPLRPDLTTTSVFSNVFYCFNPEKFS
                     YRAALWQLDRQEKSAGSFLAGLSLRVHRMEGDTGKSLIPNQVKNLFKPEDRIIAQAVT
                     NFNFNVGYVHTFVVKHSWFLTLYLVPGISIQNSYYLSEDKQIRNQQNKVTGSSEFRFI
                     LGYNGDRWYSGLSSYSISFSGNRELGVWVDDNYNWFRFFVGYRLRKVDRSRHPDWRQK
                     IGL"
     gene            complement(4649..>6108)
                     /locus_tag="PK281_08190"
     CDS             complement(4649..>6108)
                     /locus_tag="PK281_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017494536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOY49365.1"
                     /translation="LVVAGNFNLTNVDEQFQQLLDLGQHPKEMADTVTVLEKIGHFTD
                     MENERLFREFKRQGHSNQEISQLIARDLDVAEILRQASKRFDGGYVIAGMMGHGDAFV
                     LRDPNGIRPCFFMQNDEIVVVTSERPVLQTAFNIPIENVQELTPGHALIIKRNGEVSM
                     PEIRSPKPRTSCSFERIYFSRGSDADIYQERKALGHNISRKVLESLKYDIDNTVFSFI
                     PNTAETAFFGMVEGANEWLNGEKRKRILDLNGELSDERLREVLAMKIRTEKLAVKDAK
                     LRTFIADDLHRDEMVAHVYDVTYGVVRPGKDTLVIMDDSIVRGTTLKQSILRIMDRLK
                     PKKVIIVSSAPQIRYPDCYGIDMAKLGDFIAFQAAIALHKERGTEYIVEEVYEQCVAQ
                     DAFAKEEMKNVVKRIYDSFSEQEISDKIAQLVTPSDMNAEVEVIYQNLEDLHEACPGH
                     TGDWYFSGNYPTPGGNKVVNRAFINFFNKVNERAY"
BASE COUNT         1684 a         1205 c         1281 g         1938 t
ORIGIN      
        1 tccagtatgg ttttaggagt tgagttgggt tatgattttc cacaagtggc taagccgctt
       61 aaagtactga gtgatatctt ccttcgtctc attaaaacca ttattgctcc tttacttttt
      121 tccacgcttg ttgtgggtat tgctggacat tccagtttaa agcaagtggg gcgaatgggc
      181 ttgaaagcaa tcctttattt tgaaatcgtt accacatttg ctctattcat cggattggct
      241 gccatcaatt ttaccggtgc cgggaaggga gccgtttttc aagctcaaga tgctcaagtt
      301 gagaaggcga atagcttgat aagtcagaaa cagagtcatg atgtgattct ggatattttt
      361 cccgagaaca ttgcaaagtc gattttcgac aatcagatat tacagattgt ggtattcagc
      421 attttgtttg ggattggctt agccatggtg aagaatgagg ataaaaaacg tccgatgtta
      481 gcttttgcag aaagtttgtc ggaagtgatg tttaagttca cccatttggt gatgtacttg
      541 gcaccattag ctgttggtgg ggcagtagct tattcggttg ccaccatggg attgggagtt
      601 ttgcagaatc tcttcatgct ggtagctact ctttacggag cattaggcgc atttgtattg
      661 ttgattttgg tgcctattgg attaattgtt cgactaccat ttaagcgttt tttggaggca
      721 atctcggagc ctgtttcgat tgcatttgct acagcaagtt cagaagcagc tttgccgaaa
      781 gccatggaga acttagaagc tatgggcatt ccacgtaagg tagtggcttt tgtattgcca
      841 accggttata gtttcaactt agatggaaca actttgtact tatcgttggc ttcggttttt
      901 gtggctcaag tatgtggagt tgatctgagt ttatcggaac aaatacagat gtgtttaata
      961 ttaatgctaa caagtaaagg tgtggctgga gtgcgaggcg cttcattttt aattctggta
     1021 agtacggtag catccttagg tttagatcct cagaaagcct ttgctattct agcgattgat
     1081 gctgtgatgg atatgggtag aacctcagtg aatgtggtgg ggaattgttt ggcaacttat
     1141 gtggttgcac gttgggagaa agaaatctga ggattttatt aacataacct gtaagaaatt
     1201 catcttgttt tttcacccgt actggttgtt tattagtaaa ttgcaagccg aatttttaat
     1261 ggggaaaatg aaaaaactat ctacgctgtt tttgagtacg ttgatcatca catcagcctt
     1321 tgctcaacaa caagttcatc gttgtgccac tatggaagtg gacaatcgtt taaaacaaac
     1381 tcatccagag tacgctgctg cgagggaaca aattgaagaa catgctcgtg catttgctat
     1441 tcatggttct ggtcaacgtc aattagttac cattccggta gtttttcacg tagtgtacag
     1501 aaatgcaaca caaaacattt ccgatgctgc attgctttca caaatcgaca ttctaaatga
     1561 ggatttccgc aaattgaatg ctgatttcag tggaacacct tctgtttttc aaggtgctgc
     1621 agccgattta gaaatccaat tttgtctagc tacagttgat ccaaacggaa atgctacaac
     1681 tggaattaca cgtactttaa ctaccgataa tggcttcgat gatgatgatc aagtaaagta
     1741 cacttctcaa ggtggacatg atatttggaa tcgcaataaa tacttaaacc tttgggtgtg
     1801 cgatttgagt tctgctggct tattgggtta tgctcaattt cctggtggtc ctgcagctac
     1861 agatggtgta gttattacgt atgatgctat tggtcgtccg ccagcaaaca actttggtgg
     1921 agcatataat ttaggtcgta cagcaaccca tgagattggt cactggttga atttatatca
     1981 tgtttggggt gatagtaatt gcggtaatga tcaagtatct gatacaccta ctcaacaaac
     2041 ggcaaactat ggatgtaaaa catttccaca cgtaacatgt aacaatggtc cgaatggaga
     2101 tatgtttatg aactacatgg attatggtga tgatgattgt ttgaaaatgt ttaccaatgg
     2161 acaaaagact cgtgcacagg cattgtttgc aacaggtggt tcacgagtaa gtttgcttac
     2221 gtctaatgtg tgttcatctg tacctcctgt tactacttgt tcagatacat taaactatcc
     2281 tcttaatgga acagttgtta cctatacagc gagtgcaacc ttgtctgatg ggtatgtatg
     2341 tggaacaaat ctatatgaag atgttgccaa agcagactgg tttacagcta cagctccgct
     2401 tacatcttta gctggggcat tatttacttt cggagcagca tctcaaggta cagcgccagc
     2461 aggatcaacc ataacagtaa aagcatataa ctacacagct tctggtcctg gttccgtaat
     2521 tggaacagct tcgattccac tttcaaccat catagccaat gtgaatgcta atcaaaatac
     2581 ctatgtggcc tttccatcac ccataaccgt tacaggtaat tttttcctcg gaattgaatt
     2641 taatccaact gttggttatg ttttgggatt aaaaagcaat tcagatggag atgcaactgt
     2701 aaataaagca tgggaacaat ttggtgattt aagttggcat caattcactg aaaatccagc
     2761 tagttggggg atttcaatca accaagccat ttttcctatt ttaacttctg ctagtccggt
     2821 agctacattt agtccaagtg ttacctctat ttgtacaggt caatcggtta cttatacacc
     2881 agtagctgga gctgcttcct attcttggac atttacaggt ggatctcctt cttcatcaac
     2941 acaacaaaat ccgacagtta cttataattc aacgggttca tttggagcat ctcttactgt
     3001 tactggtgct tgttctggac aaagttctag tcaaacacag aatagtcttg ttactgtaac
     3061 tcaatcacct gcattgccag taatcaatat taacggtacg gaattatcag tatcaataac
     3121 tggtgtcacc tatcagtggt ttttaaatgg taacgctata tcaggagcta cttcatcaac
     3181 ttatactcct accgctgctg gttcatatac tgttcgtgtg gcaaatggaa attgtactaa
     3241 aacttcatca gccattaatt tcaatccttt agaaatcgag actgttggag atattcaact
     3301 tagtgttttc cctaatccag ctaacgattt attatcggtg aaagctgtat tctcgaatca
     3361 acaggatgtt gttgattgtc gcttgttcga taacagtggg aaattagtat tacaccgtac
     3421 ttttgagcac atccaacctg atgcgcttct gcagtttggt atttcggaga ttagcagtgg
     3481 tatgtaccaa ttggtcttaa caactgcaac cggccactct gtatcgcgcg tcgcaatcgc
     3541 tcgataaaac caatgaattc aatccccttg tcgctttcgg tcagtctccg cagcgtcagg
     3601 gggatttttt tatgctgttg cttgctgatt tcaatggctt ttaaagccca agaacaacct
     3661 cggaaatgga cacgtgatac gaactatgtt acgtctttta ctgatcagcc acatattacc
     3721 tttgaattag cgaaaaggaa tcagcgtatc gacattcgaa atcctttact tgatacattt
     3781 cttcttcgct atgaacccaa ttccaaaaca aatcttattg gttcaatcga ctaccgttgg
     3841 ttaagcttat ccttaggatt agttactata caaagtggtg atagctaccg gaaaggctca
     3901 tccactcagt tttcgttacg tgcttccttt aatggacgaa gaatctggaa ttccaatttc
     3961 attcaggtaa taaaaggtta ttatttgacc aatccattaa tggttgatcc atactggaat
     4021 cctttagtag atcaatatcc gcttcgacca gatttaacaa cgacatccgt ttttagtaat
     4081 gtcttttatt gttttaatcc agagaaattc tcctatcgtg cggcattgtg gcaattagat
     4141 cggcaagaaa aaagtgctgg aagtttcctt gctggtttat ctttacgggt tcatagaatg
     4201 gaaggagata cagggaaatc gttgatcccc aatcaggtta aaaatctctt taaacccgaa
     4261 gatcgaatca tcgcgcaagc agtaacaaat ttcaatttca atgttggata tgtgcacacg
     4321 tttgttgtta aacattcgtg gtttctaaca ctttatcttg ttccgggtat ttcgattcaa
     4381 aacagttatt atttatcgga ggataaacaa atccgaaatc aacaaaacaa agtaacaggc
     4441 tcttcggaat ttcgatttat tctaggatac aatggtgata gatggtacag cgggttgtcg
     4501 agctatagca tatctttctc cggaaaccga gaattaggag tttgggttga tgataattat
     4561 aactggttcc gattttttgt cggttaccga ctacgcaaag tcgatcgaag tcgacatcca
     4621 gattggagac aaaaaatagg cttataaact agtaagcacg ttcgttcact ttgttgaaga
     4681 agttgatgaa tgcacgattt accactttgt ttccacctgg agtagggtag ttcccactga
     4741 aataccaatc gcccgtatgg cccggacaag cttcgtgtaa atcttccaaa ttctggtaga
     4801 taacttccac ttcggcattc atatcacttg gagtaacgag ttgggcaatc ttatcggaga
     4861 tttcttgttc cgaaaatgag tcgtaaatgc gcttcaccac gtttttcatt tcctcctttg
     4921 caaatgcatc ttgcgctaca cattgttcat atacttcttc tacaatgtat tcggttccac
     4981 gttctttatg aagagcaata gctgcttgaa aagcaatgaa atcgcctagc ttagccatgt
     5041 ctattccata gcaatcggga taacgaattt gaggagccga tgaaacgata atcacctttt
     5101 ttggcttcaa ccgatccatt attcttagaa tactctgttt aagcgttgtg ccgcgaacga
     5161 ttgaatcgtc cataatgacc aatgtgtctt ttcctggacg aacaactcca tacgtaacat
     5221 catatacgtg cgctaccatt tcgtcgcgat gaagatcatc agcaatgaag gttcgcagtt
     5281 tagcatcttt gacagctaac ttttcggtgc gaattttcat cgctaatact tcccgaagac
     5341 gctcgtctga aagttccccg ttgaggtcaa gaatccgttt acgtttttcc ccattaagcc
     5401 attcgttggc gccttctacc attccaaaga atgcagtttc ggcggtgttg ggaatgaagg
     5461 aaaatacggt attgtcgata tcatacttca acgattcaag cactttacgg ctgatgttat
     5521 gaccgagtgc tttacgttct tgataaatat cggcatcgct tccgcgggag aaataaatac
     5581 gttcgaaaga gcaggaagta cgaggtttcg gactacgaat ttcaggcata ctaacttccc
     5641 cgttacgctt gatgattagt gcatgaccgg gagtcaattc ttgcacattc tctattggga
     5701 tattgaaagc cgtttggagc actgggcgtt cggaagtaac tactacaatt tcgtcatttt
     5761 gcatgaagaa acatggtcga attccattgg ggtcgcgtaa aacaaacgca tcgccatgcc
     5821 ccatcattcc tgcaatcacg tatccaccat caaaacgttt agatgcttga cgtagaattt
     5881 cagccacatc gaggtcgcgt gcaataagct gagaaatttc ctgattgcta tgtccttggc
     5941 gcttaaattc ccgaaagaga cgttcattct ccatatcggt aaaatgcccg attttctcta
     6001 gaacagtaac ggtatcagcc atttctttgg gatgttgtcc caaatcgagc aattgttgaa
     6061 actgctcatc tacattggtt aggttgaaat taccagctac caccaaat
//
LOCUS       DAOOQW010000139         6101 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_15700_length_6101_cov_4.882402, whole genome shotgun sequence.
ACCESSION   DAOOQW010000139 DAOOQW010000000
VERSION     DAOOQW010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 6101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6101
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15700_length_6101_cov_4.882402"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2072)
                     /locus_tag="PK281_08195"
     CDS             complement(<1..2072)
                     /locus_tag="PK281_08195"
                     /inference="COORDINATES: protein motif:HMM:NF015226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015204 - urea transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071918 - urea transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea transporter"
                     /protein_id="HOY49366.1"
                     /translation="MGIAPGTNWGRSLKDFGEGVLMSYAQVFFSQKRALAILLMLVTF
                     VDFSAGLSGLTAVIITQLASRLLGLDHTFIRTGVYSFNSLSVGLCLGVYYTFNFPFLA
                     ILFLASLLCLLLTVWLYGVFSVKGLPFLSFPFIFTIWLILLAARSYDALELSERGIYN
                     YNELFKIGGQQFVDIMMTIENYQLPFQFGVYFRSLGAVFFQYNLIVGVVAAIALLIYS
                     RIAFTLSLLGFYTGYLFYSFVGGDINQLTYSYIGFNFILSAIAIGGFFLIPSKWSFLT
                     VMIITPLIAVLNSGLAVALLVVQLPLYSMPFNIMVVLFLYLLRMRTWPRFIHLTGIQH
                     YSPENNLYSFSTGLERFRNSTLVLMQLPFFGKWTVTQGHDGEHTHKKDWRHALDFSVL
                     DEEGNEYMNNGLDLKDYYSFQKPVLAPADGLVVDIHANIEDNQPGDVNIDNNWGNSIV
                     IKHGEHLYSQLSHLHMGSFRVSLGDYVRKGDILALLGNSGRSPSPHIHFQLQSTPYVG
                     SPTLSWPISYYLREESNQNRLICFEIPKKDDTIQNIEINPLLKDAFGFVPGQVLKFDV
                     EEGKTKTQVKWEVRVNIYNQAYIYCHTTGAYAYLLNNGTMFYFLSFNGDRKSLLYQFY
                     LGAYQVLLGYYPDVILRDQLPVNTLKLGVLRTVQDFIAPFWQFLHVQFNLEYTEIDST
                     FNPRRMVLR"
     gene            complement(2080..3438)
                     /locus_tag="PK281_08200"
     CDS             complement(2080..3438)
                     /locus_tag="PK281_08200"
                     /inference="COORDINATES: protein motif:HMM:NF014803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49367.1"
                     /translation="MTKLRYERPIITSLNAGVSNKFGTRSLHEPVSTIDGVSVKELIA
                     QYGSPVFILSERKIRNTYRDARRAFTSRYPKVQFAWSYKTNYINAVCNIFHQEGSWAE
                     VVSIFEYKKALGNGVKGKNIIFNGPEKTEEDLRLAIGNDSLIHIDHLDELYLIIELAN
                     QLKKRPRVAIRVNMDTGIYPMWDRFGFNYENGQAWDAINKIVLTDKMDLVGLHAHIGT
                     FMLSPNAYAVAANKLADLALGLRNQHKKNIEYIDLGGGFASRNTLKGSFLPGSDVIPS
                     FDDYAEAICTTLLNAGFTKDDLPMLILESGRALIDEAGYLAGSVIANKRLSDGRRALV
                     MDMGVNLLFTSFWYDHRISPVEASGHHTENAVIYGPLCMNIDILRENVTLPPLKKGDH
                     VVVHTVGAYNMTQWMQFIAMRPNVVLIDMEGKSHIIRRKETVDDLNAMEEMPAHLKSF
                     KL"
     gene            complement(3448..4491)
                     /locus_tag="PK281_08205"
     CDS             complement(3448..4491)
                     /locus_tag="PK281_08205"
                     /inference="COORDINATES: protein motif:HMM:NF026968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOY49368.1"
                     /translation="MKQQLTIAVSGLNNTDNPGPGIPVIRALRDWKGKDVRIIGISYD
                     HLEPGLYMHDLVDKSYSVPFPSEGSDNLLLRLEYIHQQEKLDFIFPNFDAELFTFMKL
                     APQLEQMGIKTFLPTLDQFEERHKYRLSEFGIKYGLNVPLSITVGSVSEVPAALQQFE
                     FPVVVKGKFYEAYIAYNIEQVYSYFNKLQSKWGLPIIFQQFVYGNEYNVTALGDGKGN
                     TIGAVPMRKQFITDKGKAWAGITLDEPKLLEMTHKLIAANKWRGGMELELIKDKEGKL
                     FIVEINPRFPAWVYLPVACGQNMPAAAVRLAFGEEVEPFTQYDVGKLFVRYSWDNIVT
                     LSDFQKISTTGEL"
     gene            complement(4488..4739)
                     /locus_tag="PK281_08210"
     CDS             complement(4488..4739)
                     /locus_tag="PK281_08210"
                     /inference="COORDINATES: protein motif:HMM:NF017239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HOY49369.1"
                     /translation="MNIRLKSNIAVSDNGFVFDSSTGDSYSVNPIGVEIIQLMKENKS
                     SEEIIQSLEERYEVDAQTIDRDLYEFVQVLLSQQLAEKL"
     gene            complement(4819..5694)
                     /locus_tag="PK281_08215"
     CDS             complement(4819..5694)
                     /locus_tag="PK281_08215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HOY49370.1"
                     /translation="MKNTIRFIINPISGGKNKTLVPQQIEQVCTKLNIQADICLSNSI
                     QETIAQAKEAVEQQYETVVAVGGDGTINLIGAQLLHSSTALGIIPMGSGNGFARSLGM
                     PFQFERAIKIIQSRKTVLIDTGTINEKPFINLSGAGFDAHIADKFQHSKSRGFFSYVN
                     IVLREFNQYQAQHYHLKLDDEQLETDAFLITVCNGPQYGNNAFIAPAALMTDGRFHIT
                     VLKKCTWANAPGLAWRLFRGTIDLSKDVEQYSASSVQIKRKNVGPINIDGEPDSEGTE
                     LNFKLIPLSLKVIVP"
     gene            complement(5691..>6101)
                     /locus_tag="PK281_08220"
     CDS             complement(5691..>6101)
                     /locus_tag="PK281_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014200584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HOY49371.1"
                     /translation="GNAYLNGKPIRVSDRSLLKDSLIVTGFPYKDEGRLEEWMRLFMH
                     LLTKSHGVRRLGSAALDLAYVACGRFEAFYEYGLSPWDVAAGTLIVQQAGGAVSDYSG
                     GNNYLFGKELLAGNGRMNEELREVIQQQFSNREA"
BASE COUNT         1829 a         1282 c         1214 g         1776 t
ORIGIN      
        1 cgtaatacca tgcgacgggg attgaacgtg ctatcaatct cggtgtattc gaggttaaat
       61 tgtacgtgaa ggaactgcca aaaaggagca atgaaatctt gaacggttct caaaacacct
      121 agttttaaag tgtttactgg aagttgatcg cgcaaaatta catctggata atagcccaaa
      181 agcacttgat aggcacctaa ataaaattgg tataacaagg attttctatc accattaaaa
      241 ctgaggaaat agaacatcgt tccgttattg agcagatagg cgtaagctcc ggttgtatgg
      301 caataaatat aagcttggtt gtagatgttc actcgaactt cccatttaac ttgggttttt
      361 gtttttcctt cttctacatc aaacttcaat acctgtcccg gaacaaaacc aaatgcatct
      421 ttcaagagcg gatttatttc gatgttctga atggtatcat ccttcttggg gatttcgaaa
      481 cagatcaatc gattttgatt tgattcttct cgcaaataat aactaattgg ccagcttaac
      541 gtgggcgaac caacataagg cgtgctttgc aattgaaagt gaatatgtgg cgaaggcgaa
      601 cgtcccgaat taccaagtaa agctagaata tcgcccttgc gaacataatc tcccaaggaa
      661 actcgaaagg aacccatgtg caaatgcgac aattgagaat acaaatgttc accatgtttg
      721 atgacgatgc tgtttcccca gttattgtca atattcacat cgcccggttg gttatcctct
      781 atgtttgcat ggatatccac cacaagtcca tcggctggcg ctaaaacggg tttttgaaac
      841 gaataatagt cctttagatc gaggccatta ttcatgtact catttccttc ctcatcgagc
      901 acactaaaat ctaaagcgtg acgccaatct ttcttatgcg tgtgttcacc atcgtgccct
      961 tgtgtaaccg tccacttacc aaaaaatggt agttgcataa ggaccagcgt cgaatttctg
     1021 aaacgctcca atccggtgga aaagctatac aggttgtttt cgggtgaata atgttgaatt
     1081 ccagttagat gaataaaccg tggccaagtt ctcatccgca gtaaatagag gaaaagaacc
     1141 accatgatgt tgaaaggcat ggaatacaag ggtagctgaa ctaccaaaag cgcaacagct
     1201 aatccactgt ttaacacggc aattaacgga gtgattatca ttaccgttag gaaagaccat
     1261 ttacttggta tgagaaaaaa tcctccaata gctatagcac tcaagataaa attgaagcct
     1321 atgtagctgt atgttaattg gttgatgtcg cctccaacaa aagaataaaa caaataaccc
     1381 gtataaaaac cgagaagcga aagtgtaaag gcaatgcgcg aataaatgag caatgcaatc
     1441 gcagcaacaa ctccaacaat gaggttatac tggaaaaaaa cagccccaag cgaccgaaag
     1501 taaactccaa actgaaaggg taattggtag ttttcgattg tcatcattat gtcgacaaac
     1561 tgctgaccac caattttgaa aagttcgttg tagttgtata tgcctcgttc gcttagttca
     1621 agagcatcgt atgatcgggc agcaagtaaa atcaaccaaa tggtgaagat aaaagggaaa
     1681 ctcagaaagg gtaatccttt caccgagaaa acaccataaa gccacaccgt taaaagcaaa
     1741 cagagcagtg aagccagaaa taaaatagct aaaaacggaa agttaaacgt gtagtaaaca
     1801 cctaaacata agccaaccga taaactattg aagctgtata cacctgttct aataaaggta
     1861 tgatctaaac cgagtagtcg agaagctaat tgggtgataa taacagctgt aagtccgctt
     1921 agtcctgcag agaaatcgac aaacgtaact agcatgagca aaatggcgag agcacgtttc
     1981 tgggaaaaga atacctgagc atagctcatc agcactccct cgccaaagtc tttaagtgat
     2041 cttccccaat ttgttccagg cgctatgccc ataagctaat tagagtttaa atgatttaag
     2101 gtgagcaggc atttcttcca ttgcattaag gtcatcaaca gtttctttgc gacggatgat
     2161 gtgcgatttg ccttccatat cgatgagtac cacattcggt ctcatggcta taaattgcat
     2221 ccactgtgtc atattatacg ctccaactgt atgaacaaca acatgatcac cttttttaag
     2281 cggaggtaaa gttacatttt ccctaagtat gtcgatgttc atacacaatg gaccatatat
     2341 aaccgcattt tctgtgtggt gaccagatgc ttctactgga gaaatacgat ggtcatacca
     2401 gaatgaggtg aaaagtaaat tcacacccat atccattacc aaagctcttc tgccatccga
     2461 aagtcgtttg ttcgcgatta ctgaaccagc caaatatcca gcttcatcaa tcaatgctcg
     2521 ccccgattcg agtatgagca ttggaagatc atcttttgta aaaccagcat tcagaagtgt
     2581 ggtgcaaatt gcctcagcat aatcatcaaa gcttggaatc acatcgctgc ccggtaagaa
     2641 cgaacctttt aaggtattac gcgaagcgaa tcctccacct aaatcgatgt attcaatatt
     2701 cttcttgtgt tgatttctta atccaagtgc caaatctgcc agtttatttg ctgcaacagc
     2761 atacgcattg ggcgaaagca tgaatgttcc aatatgcgca tgcaatccaa ccaaatccat
     2821 tttatcggtt agaacaattt tattgattgc atcccaggct tgtccgtttt cgtaattgaa
     2881 tccgaaccga tcccacatgg gataaatacc ggtatccata tttactcgaa tggcaactct
     2941 tgggcgtttc ttaagctggt tcgccaactc aataattaaa tagagctcat ccagatggtc
     3001 aatgtgaatg agtgaatcgt ttccaatggc gagtcgtaaa tcttcttcag ttttttcggg
     3061 accattaaaa atgatgttct ttccttttac cccattccca agcgcttttt tgtattcaaa
     3121 tatcgaaact acttctgccc aagaaccttc ttgatgaaag atgttgcaga ccgcattgat
     3181 gtaattggtt ttatacgacc aagcgaattg cacttttgga taacgtgaag taaacgcacg
     3241 acgcgcatcc cgataggtat tacggatttt acgttccgat agtatgaaaa ccggtgaacc
     3301 atattgagca atcagttctt ttactgatac tccatcaatg gtacttactg gttcgtgaag
     3361 agatcttgtt ccaaatttat tactcacacc tgcattgagc gaagtgatga ttggacgttc
     3421 atatcttaat ttagtcatga ctcaatttta taattctcca gtggtagaaa tcttttgaaa
     3481 atcggaaagc gtaacgatgt tatcccaaga ataacgcaca aataacttcc ctacatcgta
     3541 ctgtgtgaaa ggctcaacct cttctccaaa agcaagtcga acagctgctg cgggcatatt
     3601 ttgaccacaa gcaacgggta aatacaccca agcaggaaaa cgtggattga tttcaacgat
     3661 gaatagtttg ccttctttgt ctttgatgag ttcgagctcc attccgcctc gccatttatt
     3721 agcagcgatg agtttatgag tcatctcgag gagtttgggt tcgtcgagcg taattccagc
     3781 ccaagctttt cctttatcgg tgatgaattg ttttcgcatg ggaacagcac caatcgtatt
     3841 cccttttcca tcgcccaagg ctgttacatt gtattcgttc ccgtaaacga actgctggaa
     3901 aataatggga agtccccatt tagattgaag cttattgaaa tacgaataca cctgttcgat
     3961 gttataagca atgtaggctt cgtagaattt acctttcacc acaaccggaa attcgaactg
     4021 ttgaagtgca gcaggtactt cgctgaccga accaacagtt atcgaaagtg gaacattgag
     4081 tccgtatttt attccgaatt ccgacaggcg atatttatgt cgttcctcaa attggtcaag
     4141 cgtaggaagg aaggttttaa ttcccatctg ttcgagttga ggtgcgagtt tcatgaaggt
     4201 gaaaagctct gcatcgaaat tggggaatat gaaatcgagt ttttcttgct gatggatata
     4261 ttctagacga agaagcaaat tgtcggaacc ttcggatgga aatggaaccg agtaactttt
     4321 atcaaccaaa tcgtgcatat acaatccggg ttcaagatga tcgtaggata ttccaatgat
     4381 gcgaacatct tttcctttcc aatcgcggag agcacgaatg actggaattc cagggccggg
     4441 attatcggtg ttgttcaatc ccgaaacggc aatggtgagt tgttgcttca tagtttctct
     4501 gctaattgct gactcaataa aacctgaaca aactcataca gatcgcgatc tatcgtttga
     4561 gcatccactt catatctttc ttctaacgat tgaataatct cctcagatga tttattctct
     4621 ttcattaact ggataatttc tactccaatc gggttgactg aataagaatc tcccgtgctg
     4681 gaatcgaaaa caaagccatt atcgctcaca gcaatattgc tttttagtcg gatattcata
     4741 agatttaggt taagcccttt cgggaattgc gtgtgcttga ccgaaggttt tgcaagaagt
     4801 ttaacgtatc gagaaacttt atggaacaat aacctttagt gacagcggta tcagcttgaa
     4861 atttagttca gtaccttctg aatcgggttc gccatcaatg ttgattgggc caacattttt
     4921 tctctttatt tggacagatg atgctgaata ttgttctaca tccttggata aatcgatcgt
     4981 gccgcgaaat aatcgccaag ctaaaccagg agcattcgcc caagtacatt tttttagaac
     5041 ggtaatatga aatcgtccgt cagtcatcaa agctgctgga gcgatgaaag cattattgcc
     5101 atattgtgga ccattgcaca cggtgattaa gaacgcatct gtctcaagct gctcgtcatc
     5161 tagttttagg tgataatgtt gagcttgata ttgattgaat tctcgcaata cgatgttgac
     5221 atacgaaaag aatccacggc ttttagaatg ttgaaatttg tcagcgatgt gtgcatcaaa
     5281 tccggcaccg gataggttta tgaagggttt ttcattaatc gttcctgtat caatcaatac
     5341 cgttttacgt gattggataa tctttatcgc tcgttcaaat tgaaaaggca ttcctagact
     5401 tcgagcaaat ccatttcctg atcccatagg aatgattccc aaggcagttg aagaatgcag
     5461 taattgagca ccaatcaggt taattgttcc atcgccaccc acggcaacca ccgtttcgta
     5521 ttgctgctcg actgcttctt tcgcttgtgc aattgtttct tgtatcgagt tactcaaaca
     5581 aatatccgct tgtatgttca gctttgtgca tacttgctca atttgctgag ggacaagcgt
     5641 cttgtttttt cctcccgaaa tgggattgat gataaaacga atggtgtttt tcacgcctct
     5701 cgattcgaaa actgctgttg gattacttct cggagttctt cgttcattcg gccatttcct
     5761 gctaaaagct ctttgccaaa taggtaatta tttccacccg aataatcact tactgctcca
     5821 ccagcttgtt gaacgattaa tgttccagcg gccacgtccc atggacttaa accatattca
     5881 taaaaagctt caaaacgacc acatgctaca tacgctaaat cgagtgcagc tgaacctaat
     5941 cgtcgaacac cgtgtgattt tgtgagcaga tgcataaata ggcgcatcca ttcttctaat
     6001 ctaccttcat ctttatatgg gaaaccagta acgataaggg aatcctttaa tagacttctg
     6061 tccgaaacac gaattggttt gccatttaaa taggcatttc c
//
LOCUS       DAOOQW010000140         6076 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_15789_length_6076_cov_6.465869, whole genome shotgun sequence.
ACCESSION   DAOOQW010000140 DAOOQW010000000
VERSION     DAOOQW010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 6076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6076
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15789_length_6076_cov_6.465869"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..719)
                     /gene="purQ"
                     /locus_tag="PK281_08225"
     CDS             complement(<1..719)
                     /gene="purQ"
                     /locus_tag="PK281_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HOY49372.1"
                     /translation="MSKLFRIGVVVFPGSNCDQDMVDALSVYDQVEVKKLWHKDHDLE
                     GSDLIVLPGGFSYGDYLRSGAIARFSPIMKEVISFASQGGYLLGVCNGFQILCESHLL
                     PGALLHNTSHRFICKNVFIKPVSTQTPITRHLNPEQGLVIPIAHGEGRYTTDADSLKR
                     LQDQDQILFRYCSEEGIIGEDFNPNGSMDHIAGVCNQGKNVFGMMPHPERAAAGLLGN
                     TDGEHILKGFLQDLSGQELISS"
     gene            complement(731..1732)
                     /locus_tag="PK281_08230"
     CDS             complement(731..1732)
                     /locus_tag="PK281_08230"
                     /inference="COORDINATES: protein motif:HMM:NF040411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6427 family protein"
                     /protein_id="HOY49373.1"
                     /translation="MFINFFRSAHFINLLMLPLVLMVMWVFILPKPEVSNTENAFPLF
                     LYAFQSLRENRILLIGSSLLLVVMQAFFLTSVINRQGVLRDNSHLPALLYVVLMSCFP
                     EQLSFNPLLFANFFIIIFLNSLFNFYRSDTAVFEVFDAGLFIGLASLFYWPCLFLFPL
                     TLASLFVLRPFEWREWLVSFVGVSLPFLFFGTILFWFDMLSLNSIRAILEPFYKVQFS
                     TAYNGTYIILTAILAVIVIASLWRFSRDLNTFAKLRTRKFLAIIVWFFLFAGLSYLVA
                     SKRSMIGLSFLAIPLSVIISNYFLSLKNQILAELMFLLLLAAVIYNQVLFFLQFTTL"
     gene            2119..>2981
                     /locus_tag="PK281_08235"
     CDS             2119..>2981
                     /locus_tag="PK281_08235"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HOY49374.1"
                     /translation="MNKLTLPFFLVILICLTSQSSAQITRVKGTVKDASTKETMPFVN
                     LLFKGTTIGTSTDINGVYELQAQRACDTLLVSFMGYKTQKIFIQRNKSNEINILLQPE
                     NITLREVVIRPGENPAHRIMREVIEHKKDNNPEKVKTYQYEVYNKIQFDLNNFKEDFT
                     KKKLFKPFDFVFTYADTTEDGKPYLPIAFTETYAEVTYTSDPARKQENIKASQISGIK
                     NASVSQFLGDMYQNVNIYDNFLLIFNKSFVSPVTDNFLRYYKYYLVDSVYIGNERCYK
                     LLFLPKRKQDLT"
     assembly_gap    2982..3081
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3082..4748
                     /locus_tag="PK281_08240"
     CDS             <3082..4748
                     /locus_tag="PK281_08240"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HOY49375.1"
                     /translation="NERCYKLLFLPKRKQDLTFTGNLFIHDSTFAVKQVNIRFNDNAN
                     INYIKQFDVFQEYERVDGKQWMLTYEKSLGDFAPFEKANGKGVFGRKTTRYQNFKINH
                     QIPDSIFKAGRDIIVSDSADQHDERYWNKLRPDTLTQKERDIYTMVDSLKQVPRLINY
                     RYLGQMLATGFLHSKWMEIGKYYTFVSWNDIEGVRLKFGGITSNDFSKRLELRGWVAY
                     GLQDNRFKVRSGLRYFVDKDKKNRRLLTLAYKSDLEQLSLSANSLTLDNILTSMLRRT
                     ALKNVTNVEEGLAMLEYDWFPGLSNKFTVFNRRLVPLGNFAFDRKLENGDIQSIRQIT
                     TTEIGLQTRFAWGEKFISGEFDRSSIGTRYPIVTVDLGMGVPNVLSSQFSYYKARLRI
                     SDRFRINPMGYTDYVVDFGKIWGTAPYLFLELHQGSQTYALDPLAFNMMNIFEFASDR
                     YAYLFIDHHFEGFFLNKIPLMRKLKWREVVTLKSVIGDMRSENINQLVYPGGLDPQLH
                     RPYLESGFAVENIFKIVRVDALWRMTHLERPNTRAFGVRVSLSFRF"
     gene            4752..5477
                     /gene="lptB"
                     /locus_tag="PK281_08245"
     CDS             4752..5477
                     /gene="lptB"
                     /locus_tag="PK281_08245"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017494919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HOY49376.1"
                     /translation="MKLHTKNLVKRYKKRVVVNNVTVEVNQGEIVGLLGPNGAGKTTT
                     FYMTVGLIRPNEGDVFLDDLNITNLPMYKRAQLGIGYLAQEASVFRKLSVEDNIKAIL
                     ELGPLSKAEQAQKLESLLDEFSLQHIRGNRGDLLSGGERRRTEIARALASDPKFILLD
                     EPFAGVDPIAVEDIQSIVAKLKDRNIGILITDHNVHETLNITDRAYLLYEGKILKSGT
                     AEELAADENVRRVYLGMNFELRR"
     gene            5543..5794
                     /gene="purS"
                     /locus_tag="PK281_08250"
     CDS             5543..5794
                     /gene="purS"
                     /locus_tag="PK281_08250"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HOY49377.1"
                     /translation="MKFIAEIDVMPHKALLDPQGKAVTGSMKNLGLPEIGNVRIGKHI
                     QLEIEAGNEELAREKVDKACKDLLANPIMEGYHFSLIPA"
     gene            complement(5809..>6076)
                     /locus_tag="PK281_08255"
     CDS             complement(5809..>6076)
                     /locus_tag="PK281_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49378.1"
                     /translation="HNGTYTFFNAWTLSGLSVLCSFLLVSEIPMFSFKLKSLSWKGNE
                     AQYSLLLLSIPFFIWLKLAALAPFVGIYVLISLVQKLQAKPEIS"
BASE COUNT         1976 a         1231 c         1151 g         1618 t
ORIGIN      
        1 gagctgataa gttcttgtcc agataagtct tgcaagaaac ctttaagtat gtgttcaccg
       61 tccgtgttac cgagtaaacc ggcagcagca cgctcaggat gtggcatcat accgaataca
      121 tttttccctt ggttgcaaac accagcaatg tggtccatcg aaccattcgg attaaaatct
      181 tctccaataa ttccttcttc actgcagtag cgaaacagaa tttgatcctg atcttgtaat
      241 cgtttcaatg aatcagcatc ggtagtatat cgaccttcgc catgtgcaat cggaatgacc
      301 aaaccttgct caggattcaa atgacgcgtt attggtgttt gagtagatac tggtttaatg
      361 aatacgttct tgcagataaa tcggtgagaa gtattgtgca acaaagctcc cggtaacaaa
      421 tgcgattcac ataaaatctg aaatccgtta cacactccga gtaaatatcc gccttgagaa
      481 gcaaatgaaa ttacttcttt catgataggc gaaaagcgag caatagcgcc cgaacgtaaa
      541 taatcaccgt aggaaaatcc gccaggtaaa acaatcaaat cggatccttc cagatcatga
      601 tccttatgcc ataatttctt cacctcaacc tgatcgtaaa ccgacaaagc atccaccata
      661 tcctgatcgc agttagatcc aggaaaaact actactccaa tacgaaaaag cttagacata
      721 attctgttgg ttacaaggtg gtgaattgaa ggaaaaagag cacttgatta taaatcacag
      781 ctgccagcaa aagtaaaaac atcagctcag ctagaatctg attttttaaa gaaagaaagt
      841 aattcgagat gattactgat aatggtatcg ccaaaaacga taaaccaatc atgcttcgct
      901 tagatgcaac gagataactt aagccagcaa ataaaaagaa ccaaacgatg atggctaaaa
      961 atttacgtgt tctcagctta gcgaacgtgt tcaaatcgcg cgaaaaacgc caaagtgatg
     1021 caataacaat aacggctagg atggctgtta agatgatgta cgttccatta taagcagtgg
     1081 aaaactggac tttatagaaa ggttccagaa tcgctcgaat actattcaaa ctgagcatat
     1141 cgaaccaaaa caaaatggtt ccaaaaaata aaaagggtag tgatactccg acaaaactca
     1201 ccaaccattc tctccactca aatggacgca aaacaaacag tgacgccaga gttaaaggga
     1261 ataaaaacag acatggccag taaaacaacg aagctaaacc gataaaaagt ccagcatcga
     1321 aaacttcaaa aacggctgtg tcggaacggt aaaaattgaa gagtgaattg aggaatatga
     1381 tgatgaagaa attcgcaaac aaaagtggat taaaggacaa ttgttctggg aaacaactca
     1441 tcaaaaccac ataaagtaat gctggcaaat gcgaattatc acgcaaaact ccttgtcgat
     1501 ttatgaccga tgtgaggaaa aaagcctgca tgactaccaa caataatgag cttcctatga
     1561 gcaagattct attttcccgc aacgattgaa acgcatacaa gaacaatgga aaggcattct
     1621 ccgtattact aacttcaggc tttggtagaa tgaaaaccca catgaccatg agtaccaatg
     1681 gcaacatgag caggttgata aaatgagcag acctgaaaaa attaataaac acggaacagg
     1741 aaaaagagag gagtcaggaa aattcttact ttcgcaacaa atttatcata atcatggata
     1801 cgctggttta cgcttgtcaa gacttttttg aatttacttt cggcttcatg ccttctattg
     1861 gcactgccgt taacctgatt tttatcgggc taatctcatt tttcatggtt tattggatag
     1921 tacaaatgtt caaaaaccca gaaaaacaac attgattttt aatacaactc cttattagtc
     1981 aaaagccctg gtctctaccg gggttttttg ttttaattca cgaagcaatg tgaacacatc
     2041 gcatatatgc agtgttcgtt aggtatcgat taaactaaat ttgctctgca tagttctagt
     2101 caaaagtttt gaattgcttt gaacaaactt actcttccct tttttcttgt tatactaatc
     2161 tgcctcacta gtcaatctag tgcgcaaatt actcgtgtaa aaggaacagt aaaagatgca
     2221 tctaccaagg agactatgcc atttgtcaat ctcctattca aaggcactac cattggtaca
     2281 tctaccgata taaatggtgt atacgaactt caagctcaac gtgcttgtga tacacttcta
     2341 gttagcttca tgggatataa gactcaaaaa atctttatcc aacgaaataa atccaacgaa
     2401 ataaatattc tgcttcagcc cgaaaacatt acacttcggg aagtagttat tcgaccggga
     2461 gaaaatccgg ctcatcgaat catgcgggag gttattgaac ataaaaagga caacaatccc
     2521 gaaaaagtta aaacctatca gtacgaagtc tacaataaaa tccagttcga cctcaacaac
     2581 ttcaaagaag acttcaccaa aaagaaactc ttcaaacctt tcgattttgt ctttacgtat
     2641 gccgacacaa ccgaagacgg caagccatat ctacccattg ccttcacaga aacatacgcc
     2701 gaagtcacct ataccagcga cccggctcgt aaacaagaaa acatcaaggc aagccagatt
     2761 tcaggaatca aaaacgccag tgtctcccag ttcttgggcg atatgtacca aaacgtcaat
     2821 atctacgaca acttcctgct gatctttaat aaaagcttcg ttagtcctgt aactgataat
     2881 ttccttcgct attacaaata ttacttggta gatagcgtat acattggaaa cgagcgttgt
     2941 tacaaattac tcttccttcc caaacggaag caagacctaa cnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn ngaaacgagc gttgttacaa attactcttc cttcccaaac
     3121 ggaagcaaga cctaacattt accggaaatc tcttcattca cgattcgact tttgccgtca
     3181 aacaggtaaa tattcgtttt aacgataatg caaacatcaa ctatatcaag cagttcgatg
     3241 tgtttcagga atatgaacgg gttgatggaa aacaatggat gctcacctat gaaaaatcat
     3301 taggcgattt tgctccattc gaaaaagcta atggaaaggg agtatttgga aggaaaacga
     3361 cccgttacca aaacttcaaa ataaaccatc aaattcctga ttccatattc aaagcaggaa
     3421 gagatataat cgtcagtgat agtgccgatc agcacgatga acgttattgg aataaacttc
     3481 gccccgatac actcacccaa aaagaacgcg atatatacac gatggtcgat tcgcttaaac
     3541 aagtgcctcg attaatcaac taccgctacc tcggacaaat gctcgcaacc ggtttcttgc
     3601 attcaaaatg gatggagatt gggaagtact acacatttgt gagttggaac gatattgaag
     3661 gagtacgtct taagtttggt ggaatcacgt ccaacgactt tagcaaacgt ctcgaactcc
     3721 gtggttgggt tgcttatggt ttgcaggata atcgtttcaa ggttagaagt ggattgcgct
     3781 actttgtaga taaagacaag aaaaatcgtc gcttactcac cctcgcgtac aaatcggatc
     3841 tggaacaatt gagtttgagt gccaactcct taacgctcga taacatccta acctccatgc
     3901 ttcgtcgtac tgcattgaaa aatgtgacga atgtagaaga agggctggca atgctcgaat
     3961 acgattggtt tccaggcttg agcaacaaat tcaccgtgtt caatcgacga ttagttccgc
     4021 tggggaattt tgcttttgat cgaaaactcg aaaatggcga tatccaatcc attagacaaa
     4081 tcactactac cgaaattggt cttcaaacac gatttgcttg gggtgaaaaa tttatctcag
     4141 gagaattcga tcgttctagc attggaacac gatatcctat tgttaccgtt gatctcggca
     4201 tgggtgttcc aaatgtgcta agttctcagt ttagctatta taaagctcgc ttgcggatta
     4261 gtgatcgttt ccgaatcaat ccaatgggtt acaccgatta tgtggtcgac tttggtaaaa
     4321 tttggggaac ggctccttat ctatttctcg aattacatca aggttctcaa acctacgccc
     4381 tcgatccact tgcattcaac atgatgaaca tcttcgaatt tgcaagcgat cgatatgctt
     4441 acttatttat tgatcaccat tttgaaggat tctttttgaa taaaatacct ttgatgcgca
     4501 aattaaaatg gcgagaagtc gttacactca aatcggttat tggggatatg cgctcagaga
     4561 atataaatca attagtgtat cccggaggat tggatcctca acttcaccgt ccatatttgg
     4621 aaagtgggtt tgccgtggag aacattttta aaattgtacg ggttgatgcc ctttggagaa
     4681 tgacacatct cgaacgccct aatacccgtg cttttggtgt gcgggtttca ctttcattcc
     4741 gcttctgacg catgaaactg cacactaaaa atttggtgaa acgttacaaa aaacgagtag
     4801 tcgttaataa cgttactgta gaagtgaacc aaggtgaaat tgttggttta ttgggaccta
     4861 atggagctgg taaaaccaca actttctaca tgacagtggg tttaattcgt cccaacgaag
     4921 gcgatgtttt tctcgatgat ttaaatatca ctaacctgcc catgtacaaa cgtgcacaat
     4981 tgggaattgg ttatttagcc caggaagcat cggtatttcg aaagctgagt gtggaagata
     5041 acatcaaagc cattcttgaa cttggtccat tatctaaagc agaacaagct cagaaactcg
     5101 agtcgctttt agatgagttt agtttgcaac acattcgcgg aaatcgagga gatcttttat
     5161 ctggcggaga acgccgacgc accgaaatcg cacgagcctt ggcatccgac cccaagttta
     5221 ttttgctcga tgagcctttt gcaggtgtgg atccaattgc tgtggaagac atccagagca
     5281 ttgtggctaa attgaaagac cggaatattg gcattcttat caccgatcat aatgtgcacg
     5341 aaacactcaa tattaccgat cgtgcctatt tactatacga agggaaaatt cttaagtcgg
     5401 gtacagccga agaattagca gcggatgaga acgttagaag agtttatctc gggatgaact
     5461 ttgaattacg ccgttaattt tcaatagcta caacccgaac tgaaatcaag cacgtatatt
     5521 tgctgcatat atcttctacc tcatgaaatt cattgccgaa attgacgtaa tgcctcacaa
     5581 agcactttta gatcctcaag gaaaagctgt tactggtagc atgaaaaatc ttggacttcc
     5641 tgaaattggc aatgttcgca ttggcaaaca tattcaactt gaaattgaag caggtaacga
     5701 agaattagct cgtgaaaaag tagataaagc ctgcaaagat ttgttagcca atccaatcat
     5761 ggaaggctat catttctctc tgattccagc ttaattcaag tatttcgttt agcttatttc
     5821 tggcttagct tgtaattttt gcacaagcga aatcagcacg taaatcccta caaaaggagc
     5881 tagtgcagca agctttagcc agataaagaa tggaatagat aatagcaata agctatactg
     5941 agcctcatta cctttccaac ttaaactctt cagtttaaaa ctgaacattg gaatttcgga
     6001 caccaataaa aaagaacata gcaccgataa gccgcttaat gtccaagcat taaaaaatgt
     6061 ataagtgcca ttgtgg
//
LOCUS       DAOOQW010000141         6073 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_15805_length_6073_cov_6.031073, whole genome shotgun sequence.
ACCESSION   DAOOQW010000141 DAOOQW010000000
VERSION     DAOOQW010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 6073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6073
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15805_length_6073_cov_6.031073"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2507
                     /locus_tag="PK281_08260"
     CDS             <1..2507
                     /locus_tag="PK281_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49379.1"
                     /translation="TFTSRRLITKDLRIFVEFEYSDRNYARSLLFFANEVEQGKTTTR
                     IHVYSEQDSRNQPLQQQLNDEQKFILREAGDDPQLAFIPAVDSVGFSTDLILYDMRDT
                     VVNGTVYSNILVFSTNPDSALYKASFSNVGANKGDYVQINSIANGRVYKWVVPVNGIP
                     QGNYVPKTQLIPPIKRQVVTVGNEYRISKSFKVLSEIAYSNYDVNTFSNIGNNNNEGY
                     AFRVAMEKTQNLGKDSLPWRLVSTVSYEQVDKYFSPIERFRNVEFDRDWNLQKVISAP
                     ATEYLPRVNVNLSKSSIGTNNYLFTAFLKGEQLQATQHALNSDLHYKKFALNYAGSLT
                     DSRGSENSTLFYRHRTTFTRDIRWLQLGYKDEYEHNLLQDVLQDTLSSNSYSFFDRQV
                     FVQNVDTSKNKFNVFYRVRTDDGVLNRNLKEYAWAESYGFSMDLSKRENIQFRTTTSF
                     RRLEIRDTLLSSLKPERTLVNRIELSFRALKSSIQSTSFYEAGSGLETRKAFSYLEVQ
                     PGQGVYSWTDYNGNGLKELNEFEVSVFPDQARYIRVFTPTTDFIRIYSSSFNEVLNLK
                     APKTWSQGKGWKKFIARISTQSALRYEGRTTNTNVVEAYNPFHAGIDDPDLITLNQTL
                     RNSVFFNKSDPKFGFDLNWQRIGTKALLNNGLEARENQFSNHRIRWSPGTRFTINGET
                     RSGRKLSNTDYYSTRNYSIRYIEFEPRVSYQPNAAFRLTLSYKHNVKNNAKENGGEHS
                     LSQTAGLDLKYNVANKGSFQAKANIILVEFDGTQNTPVAFEMQEGLRNGNNYTWGITY
                     QRTLSNNMQLNFNYDGRKSPDVKTVHIGGVQVRVFF"
     gene            2643..3050
                     /locus_tag="PK281_08265"
     CDS             2643..3050
                     /locus_tag="PK281_08265"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOY49380.1"
                     /translation="MKKPKAKYPTVMLVDDSEIDNFINQKMIEGCNFADRIYVHTSSK
                     SALEFLANFERAHLPEDLIPEIIFLDINMPIMDGFQFAEEFGKSCPKIYDKIKIVFLT
                     SSLNPVDQKRAMSVRGVYSFLNKPLGKDHLDKL"
     gene            complement(3118..4569)
                     /locus_tag="PK281_08270"
     CDS             complement(3118..4569)
                     /locus_tag="PK281_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49381.1"
                     /translation="MIKFKSIVLQLKEEEYNDLCQQFVETKADKYLRLLKLYRENKYN
                     DEEIQEMLSVNTSAYYTLKSRLFDKIQDFLTNNMSTPIIDLLRKVANIPTLIYDTHRD
                     TAIAILSKLESELQNYDMPYELSSVYSALKKLHVNSPKYYEYTQLYNKHLAYTIALDK
                     AEDLLNTFMRTTGDYYTSRTKSQVDYLSLIKQEMAHICNLYESHHLQVYKAIVDVTFA
                     LYIPNSDALKDDEPVEDVLAKMEKIFTTYNKDTNYQHLQLVFNNLAFEYYHKLNLPKK
                     EARYFDEVDKKLTSFLLFNHCCFTTKFLISKIERYVELGIEGELYEQNQKLLPNYEPD
                     KNDIPNYINFSKYMATSAFYAKKYQEAVQILNNLINDISFKNYPHSEIEVKLLLALNY
                     SMINKYDPATSLLRSVTRKIREIDDEMGYENALIFYKILSLQMSSSARQVDQKIGQLF
                     SKFELLNQKSNRMLEYIKVDEAFIKALSQVVKR"
     gene            complement(4625..>6073)
                     /locus_tag="PK281_08275"
     CDS             complement(4625..>6073)
                     /locus_tag="PK281_08275"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOY49382.1"
                     /translation="ATSYSWNLGGISTSTDFEPEISFNNAGVYNIDLIATNDFGCADT
                     AHFNGIESFLVPIAGFTINQAEGCTPVSVSFDNTSYQLDNPSYQWTFGNGQTSASTDP
                     TQVYFDPGFYSVGLTVINHNGCADTLLLPQLIQVFDTLPAPVTPISRVTVVDPSTVLI
                     EWQESLAEDFGSYLLYRKNLQTGFFDLIANITDSHVLTYTDNSLNTLDNVYCYKLETS
                     DRCGYSIEADSLIEHCTINVEVITRMDNTIDVNWTPYVGKLPSQYRIYRTEESTNLTE
                     DLGTVPGDVTTYNDNTVFCPVKFRYSVKAEALNGQTHIDSDSDYDMSEPIANLFVDQQ
                     VNASRSTVVQNQFELTEWSRPAILPNRVSGYKVFRSTDNSSFIQVATIPSEQTYYLDE
                     AVDVDNEKYYYRIMATNACGLEGRYGEFSDNVVLKAEAAGDIFIKLDWTPYIGWGENG
                     VGFYVIEKQNEDGSWEVIQQVQGNVLSTVDEN"
BASE COUNT         1891 a         1128 c         1214 g         1840 t
ORIGIN      
        1 taacgtttac atcgcgccga ctgatcacga aagacttgcg gatttttgtc gagtttgaat
       61 attcggatcg aaattatgct cgatcacttt tgttttttgc aaacgaagtt gaacagggaa
      121 aaacaactac ccgaattcat gtgtattctg agcaggatag ccgaaatcag cctttgcagc
      181 aacaattaaa tgatgagcaa aaattcatac tccgcgaagc tggtgatgac ccacaattag
      241 cttttattcc agcggttgat agtgttggat tttcgaccga tttaatcttg tacgacatgc
      301 gtgacactgt cgttaatgga actgtttatt caaacatttt agtattctcg accaatccag
      361 attcggcttt atacaaagct agcttcagta atgtgggcgc gaacaaggga gattatgtgc
      421 agataaatag tatcgcaaat ggccgagtgt acaaatgggt tgttcctgta aatggaattc
      481 ctcagggtaa ttacgtacca aaaacacaac tcattccacc aataaaacga caagtggtga
      541 ctgttggaaa cgagtatcgt ataagcaaat cctttaaagt attatctgaa attgcttatt
      601 cgaattacga tgtaaataca ttttccaata ttggaaataa taataacgaa ggttatgctt
      661 ttcgggtggc gatggaaaag actcaaaacc tcggaaaaga cagtctccca tggagattgg
      721 tgagcacggt tagttatgag caggtagata aatatttcag tccgattgag cgttttagaa
      781 atgtagaatt tgatcgcgat tggaaccttc aaaaagtaat atccgcgcca gctaccgagt
      841 atttaccacg agtaaatgtg aatttaagta aaagctctat tggaacgaat aattatctat
      901 ttacggcatt tctcaaaggc gagcaattac aggccacaca gcatgcttta aattccgatt
      961 tacactataa aaaattcgcg ttgaattatg ctggtagttt aaccgattcg cgtggtagtg
     1021 aaaatagcac cttgttttat cggcaccgaa ctacgtttac ccgcgatatt cgttggttac
     1081 agcttggtta caaagacgag tatgaacaca atttattaca agatgtttta caagacacat
     1141 taagttcgaa ttcctattct ttttttgatc ggcaggtgtt tgtccaaaat gtagacacat
     1201 cgaaaaacaa gtttaatgtg ttttaccgag ttagaacgga tgatggtgtg cttaatcgaa
     1261 atttgaaaga atatgcttgg gctgagagtt atggatttag catggactta tcaaagcggg
     1321 agaatattca atttcgcaca actaccagtt ttagaagatt agaaattcgc gatacactgt
     1381 taagcagttt gaaacccgag cgaacattag tcaatcgtat tgagttgagc tttcgagctt
     1441 tgaaatccag tatacaatcg acaagtttct atgaggccgg tagcggatta gaaacacgaa
     1501 aagcctttag ctatttagaa gttcaaccgg gacaaggtgt gtattcttgg accgattata
     1561 atggaaatgg gctgaaggaa ttaaatgaat ttgaggtgtc ggttttccct gatcaggcgc
     1621 gctacattcg agtgtttacg ccaactaccg attttattcg catttattcg agttcattta
     1681 atgaagtgtt gaatttgaaa gctcctaaaa cttggtcaca aggaaaaggc tggaagaaat
     1741 ttattgcccg tatttctacc caatcagctt taagatatga aggaagaacg accaatacaa
     1801 atgtggtgga ggcttacaat ccgtttcatg ctggaattga tgatcccgat ttaattacac
     1861 tcaatcaaac cttaaggaat tcggtattct tcaataaaag cgaccctaaa tttggcttcg
     1921 atttaaactg gcaacgaatt ggaaccaaag ctttgctaaa taatggtttg gaggcgagag
     1981 aaaaccaatt tagtaatcat cgtatccgtt ggagccccgg aacacgattt acgataaatg
     2041 gtgaaacccg ttcaggaaga aagctgagca ataccgatta ctacagcaca cgaaattatt
     2101 caatccgtta catcgaattt gaaccacgcg tatcctatca gcccaatgct gcatttcgat
     2161 taacgctcag ctataagcac aacgtgaaaa acaatgcgaa ggaaaatggt ggagagcatt
     2221 ctctgtcgca aacggctgga ttagatttga aatataatgt ggcgaataaa ggaagttttc
     2281 aggcgaaggc gaatattatt ttagttgaat tcgatggaac tcaaaacacg ccagttgcat
     2341 ttgagatgca ggaaggtttg agaaatggaa ataactacac ctggggaata acctatcaac
     2401 ggaccttatc gaacaatatg caattgaatt ttaattacga cggacgaaaa tctcccgatg
     2461 taaaaacggt tcatattgga ggtgttcagg tacgtgtgtt cttctaaaag aatatcccaa
     2521 aaggagtagt aaattgttcg ttactctgtg gattataaaa aaacaggaat tggttgaagt
     2581 gcataaattc aattattttt agccctcaac aaccaaccaa aaatacgcgt tctgagatgt
     2641 cgatgaagaa gccgaaagcc aagtatccaa cggtcatgtt agtggatgac agcgagatcg
     2701 ataattttat caatcaaaaa atgatcgaag gctgcaattt cgcagaccgg atttatgtcc
     2761 acacaagtag taaaagtgct ttagagtttc tagctaactt cgaacgtgct catcttcctg
     2821 aagatttaat tcctgagatt atctttttgg atatcaacat gcctatcatg gatggtttcc
     2881 aatttgctga agaatttgga aaatcgtgtc cgaagattta cgacaaaatc aagatcgttt
     2941 tcttaacgag ttcattaaat ccagttgatc aaaaacgtgc tatgtcggtt cgtggagtgt
     3001 attccttcct caacaagcca ctcggaaaag atcatctcga taaattgtaa aatacatact
     3061 caataaaaaa gcccggaagc aatatgcaac cgggcttttt tgtttggtat cgggtgatta
     3121 tcgtttcacc acttgactta aggctttgat aaatgcttcg tctactttga tgtattcgag
     3181 catgcggttc gacttctgat tgaggagttc gaatttggag aatagttgtc caattttctg
     3241 atccacttgg cgagcagaag aactcatctg caacgagagg attttgtaga aaataagcgc
     3301 attctcataa cccatttcat catcaatttc gcgaatctta cgtgtaacgc tacgaagcaa
     3361 gctggtagct ggatcgtatt tgttaatcat cgaataattc aaggcaagaa gcagtttcac
     3421 ctcaatttcg gaatgaggat aatttttgaa actgatatcg ttgatgaggt tattcaatat
     3481 ctgaacggct tcctgatatt tcttcgcata gaaggcacta gttgccatgt atttggagaa
     3541 attgatgtag ttaggaatgt cgtttttatc aggctcgtaa ttaggaagaa gtttctggtt
     3601 ttgctcgtat aattcacctt caattcctaa ttctacatat cgttcgattt tcgaaatgag
     3661 gaatttggtc gtaaaacagc aatggttaaa taagaggaat gaagtgagtt tcttatcgac
     3721 ctcatcaaaa taacgggctt cttttttagg caaattcaat ttgtgataat attcgaatgc
     3781 cagattattg aaaaccagtt gaagatgctg atagttggtg tctttattat acgtagtgaa
     3841 aatcttctcc attttcgcca aaacatcctc taccggctca tcatctttca atgcatccga
     3901 atttggaatg tacaaggcaa acgttacatc cacaatggct ttataaacct gtaagtgatg
     3961 agattcataa agattgcaga tatgtgccat ctcctgtttg ataagagaca gataatcgac
     4021 ctgcgatttg gtacgagatg tataataatc gccggtagtt cgcataaacg tgttaagcaa
     4081 atcttcggct ttatcaagtg caatggtata ggctaaatgc ttattgtaaa gctgcgtgta
     4141 ttcgtaatac ttaggagagt taacatgcaa cttcttaagt gcactgtata cactcgacag
     4201 ttcatacggc atgtcgtaat tttgcaactc cgattcgagt ttagacaaaa tagcaatagc
     4261 cgtatcgcga tgcgtatcgt aaataagcgt tgggatattc gccacctttc tcaacaaatc
     4321 gataattggt gtcgacatgt tgttggtgag gaaatcttga atcttatcaa acagacgcga
     4381 ctttaaggta tagtaagcgc ttgtgttaac gcttaacatt tcctgaattt cttcgtcgtt
     4441 gtatttgttc tcccgatata acttcaggag tctaagatac ttgtctgcct tagtctcaac
     4501 aaactgctga cacaggtcgt tgtactcttc ttccttgagc tgcaggacaa tgctcttaaa
     4561 cttaatcatg ctcgcttttt gccgacaata tatgcgaatt ttttaattgc cgattcctgt
     4621 gtttctaatt ttcatcaaca gtgctaagaa catttccttg cacttgttgg atcacttccc
     4681 agctaccatc ctcattctgt ttctctatca cgtaaaaacc gaccccattt tctccccatc
     4741 cgatgtaagg agtccagtca agtttgataa aaatatctcc tgcagcctcg gcttttaaca
     4801 ccacattatc gctgaattca ccatatcgac cttctaatcc acaggcattt gttgccataa
     4861 ttcggtaata atacttctca ttgtctacat caactgcttc atctaagtag taagtctgtt
     4921 ccgatggaat ggttgctact tgaataaaac tgctattgtc ggtcgaacgg aatactttat
     4981 aaccactaac tcggttaggt aaaattgctg gtcgactcca ttctgttagt tcaaattggt
     5041 tctgaacaac ggttgagcga gaagcattta cttgctgatc aacaaataaa ttggcaattg
     5101 gctcactcat atcataatca ctatccgaat cgatgtgtgt ttgtccgttc aaagcctctg
     5161 cttttactga ataacggaat ttaactggac aaaaaacggt attgtcgtta tacgtagtta
     5221 catcacccgg aactgttcct aaatcttctg ttaggttagt actttcttcc gtccgataga
     5281 ttcggtattg agaaggcaat ttccccacgt aaggagtcca attcacatca atggtattat
     5341 ccattcgagt gataacttct acgttgatag tacaatgttc aattagacta tcagcttcga
     5401 tagaataacc gcagcgatcg gatgtctcca gtttgtagca atacacattg tcgagtgtat
     5461 tcaagctatt gtcggtgtag gtaagaacat gcgaatctgt aatgttagca atcaaatcaa
     5521 agaagccagt ttgcaaattc ttgcgataca gtaaataaga accaaaatct tccgccaagg
     5581 attcttgcca ttcaattaac acggttgaag gatcgacaac tgttactcgt gaaattggag
     5641 ttactggagc tggtaaagta tcaaatacct gaatgagttg aggtaacaga agtgtatcag
     5701 cacaaccatt atgattgata actgttaaac caacagaata aaatccagga tcaaaataaa
     5761 cttgagtagg atcagtcgat gctgatgttt gcccatttcc gaaagtccat tgataagatg
     5821 gattatctag ttgataagaa gtattatcaa agctcaccga aactggagta caaccttctg
     5881 cctgattaat tgtaaagcct gcaattggaa ctaaaaacga ttctattcca ttaaagtgtg
     5941 ctgtatctgc acaaccaaaa tcattggtag caataaggtc tatattataa actcctgcat
     6001 tattaaagga aatttctggt tcaaaatcag tactggttga tataccacct aaattccaag
     6061 aataactagt ggc
//
LOCUS       DAOOQW010000142         6072 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_15809_length_6072_cov_4.389729, whole genome shotgun sequence.
ACCESSION   DAOOQW010000142 DAOOQW010000000
VERSION     DAOOQW010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 6072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6072
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15809_length_6072_cov_4.389729"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..817
                     /locus_tag="PK281_08280"
     CDS             <1..817
                     /locus_tag="PK281_08280"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HOY49383.1"
                     /translation="PVLHHDSVQYERTLFVNRIIQTSYNTKNKKYNDHAYFNVTGDVL
                     SMFPKESDMLLLGLGGGVLAQDAVSRGMHVDAVELDERIIEVSRKFFGLGDEVQVIQD
                     DARHYINRTEKQYDLIIFDLFRGEETPAHVFTAESISKTRELLKPGGLVLINSNGYYR
                     NDIGKGNRSLYKTIRAMGMFTELYTTDSLEEKSNMIYLFSNSMDTFEDRVPDYIRDKF
                     IPSDSIDVKDAIILTDRRPVLDYLNEDATKVWRMGYLNYMRNYYKNYHIPLFN"
     gene            complement(810..2651)
                     /gene="asnB"
                     /locus_tag="PK281_08285"
     CDS             complement(810..2651)
                     /gene="asnB"
                     /locus_tag="PK281_08285"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HOY49384.1"
                     /translation="MCGIAGYFQTSSRLNTGHINRMTSIQKHRGPDASGHFEHPRCGL
                     GHRRLSILDLSEAANQPFYSTDGRYVIAYNGEVYNFKEIASELEVNWRTTSDTEVILE
                     AYIKWGSSFIQRMNGMFAIAIFDTQQETLFVCRDRMGIKPIFYYRDEQSFIFSSELKG
                     LLALRPDIQLSLNRQAVEEYLHLGYVPEPLSLCKEISKFPAGYYAIISSTGMELKAYW
                     EMADKILPETEKDEARAKEQLKQLVNSAVRYRMISDVPFGTFLSGGIDSSLVTAVAQN
                     NSDKPINTFSIGFLESKHNEAQFAHDVARHLGTRHHEFTVTEQDAIEMIDQFVDAYDE
                     PYADSSGIPTMLVSKLARQHVTMTLSGDGGDELFLGYGSYTWANRMANPLARLFRSPI
                     HSLLSSAKNQKYQRAAYHFEKSAHPLSHIFSQEQYYFSEQEIQQLLVKPSGTTCVEEK
                     LDSILRKLQPAEEQAFFDMRYYLKDDLLTKVDRATMQFSLETRVPLIDYRIVEFALNL
                     DSSLKLKKGIAKYLLKEVLYDYVPKSFFDRPKWGFSIPLDRWLSRELNYLIDQYTSKE
                     MCEKFGLINFDVLDRYRKEFKSGKTYLYNRIWQIIMLHKSLEHTGIN"
     gene            complement(2653..5304)
                     /locus_tag="PK281_08290"
     CDS             complement(2653..5304)
                     /locus_tag="PK281_08290"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HOY49385.1"
                     /translation="MPKIKFKALLPHLVAVAVFLLLIFTYFPELLDNKSLRMDDVEQH
                     KGMSNELVDYRERTGEEAIWTNAMFGGMPGYMISTVYHGNLLRYADKILKMGLPRPAD
                     SMFVLMLGMYVLLLSFRVKQPLAVLGAVVYCFSTFNLLSMEAGHMSKIDAMSYMPWVL
                     AGLVNAFHGKRLWGALLMAVFLSLQIKATHFQITYYLFFILGFFSITYLVDSIREKTL
                     PNFLKTSAFLVIAVLLGIVTNISSLYTIYDYGKDSIRGKSELTSKAATENSGLDASYA
                     LQYSYGVGETFSYMIPNVYGGASNLGLGEQKDALKEAEAQYKPILENLPQYWSSTSTT
                     GPFYAGALICFLAVLGFFFVKSRIKWAILASIILAFMLAWGSNFEGFSLWMLEHFPGY
                     NKFRAVKMTLIISDFLIPLIAVLGLSEIISNPEIIKEKKIGFFAAFGLTGGLSLLFYL
                     MPDFFFSFDYLNPAYHDQLMNMAQQQGMDSNSANSWMDGLISSLESVRVVIFKADAIR
                     AFLIITLGAGILFLYSRMKFNPLILAIAFLLISVADIWSVDKRYVNKKDFVTNAKSAV
                     PFSPSQADEAVFAMELQRDPSLQPKVASFTSEGIQRAKKLGVGADEAKYRFRGLLANT
                     DYRVLNLAANTFNDAGTSFFHKSIGGYSAVKLERYQEFIENHISANIESLKKAFSNRP
                     TDSSLTAAMSQLYALNMLNTRYIIYNDQAPPLVNKSALGNAWFVSNIKQVESADAELS
                     ALDSSFNPATTAIIDKRFADQLSGLNPKIDSLSSIRLESYQPNLLKYATSANSDQLAI
                     FSEIYYAKGWNAYIDGQLVPHLRANYILRGMKIPAGKHALEFRFEPETYFTTEKIGMA
                     SSGFLILLVITLLLWDIKKRNSVSEKA"
     gene            5469..>6072
                     /locus_tag="PK281_08295"
     CDS             5469..>6072
                     /locus_tag="PK281_08295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOY49386.1"
                     /translation="MKNTEIKISGAYIILGLSWIVFSKYFGSTTTSNLAAENNGNHIF
                     LSVAIIFCTGILFYFIVKYFISRLNDTLIEHRSIFENSPNPILLIEQDSLRISDVNLE
                     AIKYCGINRIELINKDIRSLIRYDAFDNLKQEIYTKKVFSGDLGVQEVIISNGCTRKV
                     NLFAHSIRLNGKESIALTLFNVAENHSFEERHYQLPIKSAR"
BASE COUNT         2038 a         1079 c         1129 g         1826 t
ORIGIN      
        1 acccgtactt catcatgatt cagtacaata tgaacgaacg ctatttgtaa atcgaatcat
       61 tcaaacttcg tataatacta aaaataagaa atacaatgac catgcgtatt tcaatgttac
      121 aggcgatgtt ttatcgatgt ttccgaaaga atccgatatg ctgttattgg gtttaggtgg
      181 cggagtttta gcacaagatg cagtaagtag aggaatgcac gtagatgctg ttgagctcga
      241 tgaacgtata attgaagtgt cgagaaaatt ctttggatta ggtgatgaag ttcaggtgat
      301 acaagatgat gctcgacatt acatcaatag aactgagaaa caatatgact tgatcatttt
      361 tgatttgttt agaggagaag aaacccctgc acatgtgttc actgcagaaa gtataagtaa
      421 aacccgagaa ttattaaagc caggtggatt agtattaatt aattcgaatg gatattaccg
      481 taatgatatt ggtaaaggaa atcggtcctt atataagaca attcgagcta tgggtatgtt
      541 tacggaatta tatacaaccg atagtttaga agaaaaaagt aatatgattt atttattttc
      601 taactcgatg gatacgtttg aagatcgagt tcccgattat attcgagata aatttatacc
      661 atctgatagt attgatgtta aagacgcaat catattaact gatcgcagac ctgttttgga
      721 ttatcttaat gaagatgcaa ccaaagtatg gagaatgggg tatttaaatt atatgcgtaa
      781 ttattacaaa aattatcaca ttcctttgtt taattaatcc ccgtatgttc caaagatttg
      841 tggagcataa ttatttgcca gattcgatta tataagtaag ttttacctga tttgaattcc
      901 tttctatatc tatctaaaac atcaaaatta attagaccaa atttctcaca catttccttg
      961 ctagtgtatt gatcaattaa ataatttaat tcacgcgata accatctatc gagcggaata
     1021 ctaaatcccc attttggtct atcgaaaaag gatttaggaa cataatcata caacacttcc
     1081 ttcaataaat atttagcaat tccttttttc aatttgagag aagaatcaag attaagggca
     1141 aattcaacaa ttcggtaatc gataagtggt actcgtgttt ctaatgagaa ttgcatagta
     1201 gcacgatcaa ctttagtcag taaatcatcc ttgagataat aacgcatatc gaaaaatgct
     1261 tgctcttcgg ctggttgcag ttttcgaagt atggaatcta atttttcttc gacacaggtt
     1321 gtacctgaag gttttacaag aagttgttgg atttcttgtt cagaaaaata atactgctct
     1381 tgagagaaaa tatggcttaa tggatgggca gacttttcaa aatgataagc agcacgctga
     1441 tatttttggt tcttagctga agaaagtaat gagtgaatag gtgatctaaa caaccgagct
     1501 aatggatttg ccattcgatt cgcccaggta taagaaccat atcctaagaa cagctcatct
     1561 ccaccatcac ccgaaagcgt catggtaaca tgttgtcgag ctagtttaga aaccaacatg
     1621 gtaggaattc cagacgaatc ggcataaggt tcatcataag catcgacaaa ttgatcgatc
     1681 atctcaattg catcttgctc agtaactgta aattcatgat ggcgagtacc taaatggcga
     1741 gccacatcat gagcaaattg cgcttcgtta tgtttacttt ctaagaatcc gatagagaag
     1801 gtattgatag gtttgtcaga gttgttttga gcaactgcag taactaaact cgaatcaatt
     1861 ccgccactta gaaaagtacc aaatggcaca tcactaatca ttcgatagcg aacagcacta
     1921 ttgacaagtt gcttgagttg ttctttagct cgagcttcgt ctttttctgt ttcgggtaat
     1981 atcttatcag ccatttccca ataggctttc aattccatac cggtcgaaga aatgatagca
     2041 taatatcctg ctggaaactt gcttatttct ttacacaagc tgagtggttc aggaacgtaa
     2101 cctaaatgca gatattcttc aacagcttgt cgatttaagc taagttgaat atcggggcgc
     2161 aaggctaata aaccttttaa ttcagaagaa aagataaatg attgctcatc tcgataatag
     2221 aaaatcggct ttatacccat ccggtcacga caaacaaaaa gcgtctcttg ctgtgtatcg
     2281 aaaatagcaa tagcaaacat tccattcatc cgctgaataa aggaagatcc ccatttaata
     2341 tatgcttcaa gaataacttc agtgtcggaa gtagtgcgcc agtttacctc aagctcagag
     2401 gcaatttctt tgaaattata tacttcaccg ttgtatgcaa tcacgtaccg gccatcggtt
     2461 gagtagaatg gttggtttgc ggcttctgat agatcgagaa tgctcaatct ccggtgtcct
     2521 aagccacaac gaggatgttc aaaatgacca gatgcatcag gaccacgatg cttctgaatt
     2581 gaagtcatgc gattgatatg cccagtattt aaccgagaag aagtctgaaa gtaccccgca
     2641 attccgcaca tattatgctt tttcgctaac tgaatttcgt tttttgatat cccaaagtag
     2701 aagggttatt accaatagaa ttaggaatcc agagcttgcc attccaattt tctcggttgt
     2761 aaaatatgtt tcaggttcaa aacggaactc cagtgcatgt ttaccagctg gaattttcat
     2821 tcctcgtaag atgtaattag cacgtaaatg agggactaat tgaccatcaa tatatgcgtt
     2881 ccaacctttt gcataataga tttctgaaaa aatggctagt tgatcggagt tggctgatgt
     2941 agcgtatttt aagagattcg gctgatagct ttcgagacga atacttgaca aagaatcaat
     3001 cttcggattc aaaccactaa gttgatcagc aaatcgtttg tctattatag cagtagttgc
     3061 tggattaaat gaactatcca aggcacttaa ttcagcatcg gccgactcaa cttgtttaat
     3121 attggagaca aaccatgcat tccctaaagc acttttatta accaaaggag gcgcttgatc
     3181 gttgtatatt atataacgag tattgagcat attaagtgca tacaattgac tcattgcagc
     3241 agtcaaactc gaatcggttg gacgattact aaatgctttt tttaaggact caatgtttgc
     3301 actgatgtga ttttcgatga attcttgata ccgttctaat tttactgcac tataaccacc
     3361 tattgattta tggaaaaatg atgttccggc atcattaaag gtattagctg caagatttaa
     3421 aacacggtaa tcggtattag ctaacaatcc acgaaagcga tattttgctt catcggctcc
     3481 aacgcctagt ttttttgctc gctgaattcc ttccgaagta aaagacgcca ctttgggttg
     3541 tagcgaagga tcgcgctgca attccatagc aaaaacggct tcatctgcct gagatggact
     3601 aaatggaact gctgattttg cgtttgttac aaaatccttt ttattaacgt agcgcttatc
     3661 aaccgaccaa atatctgcaa ccgaaataag tagaaaagcg attgctagaa tgagtggatt
     3721 aaacttcatt ctactgtata aaaagagaat tccagctcct agagtaataa taagaaaggc
     3781 acgaatggca tctgctttaa aaattaccac acgtacagat tctaaacttg aaattaaacc
     3841 atccatccat gaattggcac tattggaatc catcccttgt tgctgtgcca tattcattaa
     3901 ttgatcatgg tatgcaggat ttagatagtc aaaactgaaa aagaaatcgg gcatgagata
     3961 aaaaagcaaa ctcaaaccac cagtcaaacc gaaagcggca aaaaatccaa tctttttttc
     4021 tttgataatt tctggattag aaatgatttc cgaaagacca agaactgcaa ttaaaggaat
     4081 gagaaaatcg gaaatgatta aggtcatttt caccgctcga aacttgttgt aaccgggaaa
     4141 atgttcaagc atccaaagtg agaagccttc gaaattactt ccccacgcca acatgaaagc
     4201 taggataatg gaagctaaaa ttgcccattt aatgcgcgat ttaacgaaaa agaatcctaa
     4261 aaccgcaagg aaacaaatca atgcaccagc atagaaagga cctgtagttg aagtagaaga
     4321 ccaatattgg ggaagattct ccagtattgg tttgtattgg gcttcggctt cttttaaagc
     4381 atctttttgt tcacctaatc ctaaatttga agctccacca tatacattgg gaatcatgta
     4441 ggaaaaagtc tctccaacac catagctata ttgaagagca taagaagcat ctaagcctga
     4501 attttcggta gctgctttac ttgtaagttc agactttcca cgtatggagt ctttaccgta
     4561 gtcgtaaatt gtatagagtg aggaaatatt cgttacgatt cccaacaata cagcaattac
     4621 aagaaatgca gatgttttaa ggaagtttgg aagtgttttt tcacgaatgc tatcaactaa
     4681 ataagtaatc gagaagaaac caagaataaa gaaaaggtaa taggttattt gaaagtgagt
     4741 tgctttgatt tgaagactga gaaaaacagc catcaataag gccccccata atcttttccc
     4801 atggaaagca tttaccaatc cagctaaaac ccaaggcata taagacatag catcaatctt
     4861 agacatatgt cctgcttcca tactcagtaa attgaaagtg gaaaagcaat aaacaacagc
     4921 acctaagaca gctaaaggtt gcttcactct gaaacttaga agcaaaacat acatcccaag
     4981 cataagaacg aacatggaat ctgcagggcg aggcaatccc attttcagga ttttatcggc
     5041 atatcgtaat aagttaccat gataaacggt agaaatcata taaccaggca taccaccaaa
     5101 catggcatta gtccagattg cttcttctcc ggttcgttca cggtagtcga ctaattcgtt
     5161 tgacattccc ttatgctgct ctacatcatc catgcgaagg cttttattat caagcaattc
     5221 ggggaaataa gtaaagatta gaagtaaaaa tactgcgacc gccaccaaat gcgggagaag
     5281 tgctttgaat ttgattttag gcattcctga gatttaaaaa ggtaaatata gaagtaccaa
     5341 tttaaagaag tggtattgat tcgagatttc tgaacacttg aaagtacaaa tgcacctcaa
     5401 ccattgaaac aaaagtattt acaagtatta atatttttgt atactcgcac ctacccaacc
     5461 tcactagcat gaaaaacact gaaattaaga tttcaggtgc atatattatt ttaggattat
     5521 catggattgt tttcagtaaa tattttggtt caactactac gtccaattta gctgctgaaa
     5581 acaatggaaa ccatatcttc ttaagtgttg caataatatt ctgtactggt attttgtttt
     5641 acttcatagt aaaatatttc attagcaggc ttaacgatac gttgattgag cataggagta
     5701 tatttgaaaa tagtcccaat ccaattttac ttatagaaca agattcatta agaatttcag
     5761 atgttaattt agaagcaatt aagtattgtg gaattaatcg tatagagtta ataaacaaag
     5821 acatacgttc attaattagg tatgacgcgt ttgataattt gaagcaagag atttacacta
     5881 aaaaagtatt ttcaggtgat ttgggagtac aagaagtaat catatcaaat ggatgtacta
     5941 gaaaggtaaa tctatttgca cattccattc gtttaaatgg taaggaatca atcgcattaa
     6001 cattatttaa tgtagctgaa aatcattcat ttgaagagcg gcattaccaa cttccaataa
     6061 agtcggcaag gc
//
LOCUS       DAOOQW010000143         6041 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_15911_length_6041_cov_4.898931, whole genome shotgun sequence.
ACCESSION   DAOOQW010000143 DAOOQW010000000
VERSION     DAOOQW010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 6041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6041
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15911_length_6041_cov_4.898931"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1389)
                     /locus_tag="PK281_08300"
     CDS             complement(<1..1389)
                     /locus_tag="PK281_08300"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3536 domain-containing protein"
                     /protein_id="HOY49387.1"
                     /translation="MEKYVCIHGHFYQPPRENAWLEVIEQQDSAYPYHDWNERITYEC
                     YGPNGTSRILDKDEAIINIVNNYEKISFNFGPTLLQWMEQHTPAAYSRVLSADRVSQQ
                     HFGGHGNALAQVYNHLIMPLANRRDKETQVIWGIQDFEYRFGRKPEGMWLAETAVDTE
                     TLEVLAENNIRFTILAPRQASRVKSPDGNWIDVSGEKIDTCRPYTCKLPSGKSIVLYF
                     YDGQISQDIAFKGLLIDGKEFATRLTDKVSGSGKRLVHVATDGESYGHHHRHGDMALA
                     YCIDYIETNKLATLTNYGQFLDLVPVDWEAEIFESSSWSCVHGVERWKADCGCSSGMN
                     PGWKQTWRAPLRESLNWLRDQLSVVFEKELSRYMAEPWKARNEYIQVVLNRNEQSVND
                     FLKQHCRIVPQGDHKTRVMRLLEMQRHAMLMYTSCGWFFDEISGIETTQILQYADRAI
                     QLAESESSIVLEK"
     gene            1701..3113
                     /locus_tag="PK281_08305"
     CDS             1701..3113
                     /locus_tag="PK281_08305"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOY49388.1"
                     /translation="MNEFSEENENPEFAVKRFERMLRLNEQYYFDRDDLENIVEFYIH
                     ENHHQKALKAIKFASGQYPDSTYFILRKSQLMSADGQYEAALKLLDEIEIFEPGNPEI
                     FVTRGGIYAMLGRPAEAIEAYKAALRIDRRIEEIYLFIAFEFQNQSKYGKAARYLRRC
                     LKLNPENESALYEMSFCFDVTNKLEAGVKYFNSFLDEHPYSKVAWYALGHLHSKQQQY
                     IPAIEAFDYATVIDETFASAWFNKGNCQAASEQYEQAIESYKEIFKLEEPDAMTFLYI
                     GECYENLDKYPEALKYYQQASAMDEFLPDAWIGIGVVLDFLDRSNESLSYVKKGINLD
                     PTRGTFWFILGDLQLKLGFVNEAEESYGKVLEFEPENEDIWVFMCNLLLEQGRVDEAQ
                     TLMQECFKYHPGDARLTFQSCEVELHKGNLQEALMLAEIALATDPALGPKMQEELPRF
                     GMNAQIAQLVERYRDQEKND"
     gene            3106..3894
                     /locus_tag="PK281_08310"
     CDS             3106..3894
                     /locus_tag="PK281_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphosulfolactate synthase"
                     /protein_id="HOY49389.1"
                     /translation="MINILPYLPERSAQPRETGVTMMMDKGLSFRQAEDFIAHSGHLA
                     DVIKLGFGTSLVAKNVKEKIKLYQDAGLRVYVGGTLFEAFIIRNKFDEYRKYIDNLGL
                     DTCEVSDGSIVLDHGKKCDYISKLAKNYRVLSEVGSKEAGILIAPAKWISMMKAELDA
                     GSWKVIAEAREAGNVGIYRPNGTAHVVLVNRILSKIPSDKILWEAPVKSQQAWFIKHI
                     GANVNLGNISHDDVIPLETLRLGLRGDTFFQFLPEELHKLKQTV"
     gene            3897..4211
                     /locus_tag="PK281_08315"
     CDS             3897..4211
                     /locus_tag="PK281_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOY49390.1"
                     /translation="MQEISVTELKSWRDQGKEHQLIDVREDYEYEAANIQGELIPMGT
                     IPQHLDSIRRDIPVVLQCKSGGRSGNVTRYLMQQGFTNVYNLTGGIFAWIEQVDPSMS
                     KY"
     gene            4215..4997
                     /gene="aroE"
                     /locus_tag="PK281_08320"
     CDS             4215..4997
                     /gene="aroE"
                     /locus_tag="PK281_08320"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF020090.2"
                     /note="AroE; catalyzes the conversion of shikimate to
                     3-dehydroshikimate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HOY49391.1"
                     /translation="MRRYGLLGRKLAHTFSPAYFAQKFEQEQILDAQYETFELNGLDD
                     FPRLLTKNPGLKGLNVTIPYKTDICAYLDELFGPAEALQVVNTVVVEEVEEAFEGQKI
                     PGYRLKGFNTDIYGFKESIKPLMRPWFERALILGTGASATSVAYVLYKLGVDTLFVSR
                     NPEGEQEIGWEDINEHVIRFHPLIINTTPIGQFPDIDQAPELPYHLLTNQHLAYDLVY
                     NPAETLFLKKAKEAGAMVHNGQSMLEIQASQSWKTWQNAEKR"
     gene            complement(5000..5545)
                     /locus_tag="PK281_08325"
     CDS             complement(5000..5545)
                     /locus_tag="PK281_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49392.1"
                     /translation="MYLLNTRIILSAIVGTFLFLSACKKSAEPLSYTKEYPEMTFTVD
                     TTSSTGDMLLGVQAVQTDVVNYLSENGFTVNNLKSVKVSSIQIAVITSGKTLDFFKKL
                     EVRLSNQGAGNVIFASKELPDSFTETTTTITPSDVNLAEYFKQGELEFSFYGRNDLQI
                     LPEPIQMRAKINVQVDARLGN"
     gene            5633..>6041
                     /locus_tag="PK281_08330"
     CDS             5633..>6041
                     /locus_tag="PK281_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49393.1"
                     /translation="MLKFGLLFISVLQSACLLWAQIPFQSLADSRFYLNSKMTVHGYP
                     VPDYFEEKVFDSLITFKGEAMHEQKFSRFTTKGQSLETYPRKTYFSVNAIQEWKKNEV
                     WHSIDRSLESQKGVICGKKGTIKRSFIEQQHPKV"
BASE COUNT         1801 a         1138 c         1307 g         1795 t
ORIGIN      
        1 cttttcgagc actatactac tttcactttc ggctaactga atggctcgat cggcgtactg
       61 caaaatctga gtggtttcaa ttcccgaaat ctcatcgaaa aaccaaccac agcttgtgta
      121 catcaacata gcatgacgtt gcatttcgag caaacgcatc acccgagttt tgtgatctcc
      181 ttgtggtaca atccgacaat gttgtttcag aaaatcattc accgattgct cattccgatt
      241 cagaaccact tgaatatact cattacgagc tttccaaggt tctgccatgt agcgacttag
      301 ctctttttca aatacaaccg aaagttggtc acgaagccag tttaaggatt cacgcaaagg
      361 tgctcgccag gtttgtttcc aaccaggatt catacccgaa ctacagccac aatcagcctt
      421 ccagcgttcc acaccgtgaa cacaactcca agagctactt tcgaaaattt cggcttccca
      481 atcgactggc actaaatcga ggaattgtcc gtagttagtg agagttgcga gtttatttgt
      541 ttcgatgtag tcgatacaat aagccagagc catatcaccg tgtcgatgat ggtgaccgta
      601 actttctcca tcggttgcta catgaaccag tcttttaccg cttcccgaaa ccttatcagt
      661 aagacgcgta gcgaattctt tcccgtcgat tagtaagcct ttgaatgcaa tatcctgaga
      721 aatttgtcca tcgtagaaat acaaaacgat gctttttccc gatggtaatt tgcaggtata
      781 tgggcgacag gtatcaattt tttcacccga aacatctatc caatttccat cgggagattt
      841 cactcgagaa gcttggcgcg gagcgagaat ggtaaaacga atattgtttt cggcaagcac
      901 ttctagagtt tcggtatcga cagctgtttc ggcgagccac ataccttcgg gtttgcgacc
      961 aaatcggtat tcgaaatcct ggattcccca aatgacttga gtttctttat cacgacgatt
     1021 ggcaagcggc atgatcaagt gattgtacac ttgtgcgagt gcatttccgt gaccaccaaa
     1081 atgttgctga gaaactcgat cagccgataa cacacgactg taggctgcag gcgtatgttg
     1141 ttccatccat tgcaaaagcg ttggaccgaa attgaagctt attttctcgt agttattgac
     1201 aatgttgatg attgcttcgt ccttatcgag aatacgggaa gttccgtttg gaccgtagca
     1261 ttcgtaggta attcgttcgt tccagtcgtg atatggatag gcagaatcct gttgttcaat
     1321 tacttccagc caggcatttt cacggggcgg ctgataaaag tgaccgtgaa tacagacata
     1381 tttctccatg tatattctga atgtgccgaa atgcaactat ttcgagctga attcctattt
     1441 ggcgttggca atgttaggaa aaacttgtcg aattgacact taggcttctg ttcttgagct
     1501 caaatcgggg tgaaattgtg aataaaatca ttaaaaacgg cgacaatttg gactgaccta
     1561 aaccgttatc aatcgtgaag ttgaaaccta attaactcgt cttcaactat atgattcccg
     1621 cgaaggattt ctgttaaaaa cttccggttc caaacgggca ttatttctta gttttgcaga
     1681 aaccaattag atggcggcgt atgaatgaat tttcagaaga aaatgagaat ccggagttcg
     1741 ctgttaaacg gtttgagcga atgctgcgct tgaatgaaca gtattacttc gaccgagatg
     1801 atttagaaaa catcgttgaa ttttacattc acgagaatca tcaccaaaaa gcccttaaag
     1861 caatcaaatt tgcttctggt caatatccag attctactta tttcatcttg cgtaaatctc
     1921 agctcatgtc ggccgatggt caatatgaag ctgcgttaaa actgttggac gagattgaaa
     1981 ttttcgaacc cggcaatccc gaaatattcg ttacacgtgg tggaatttat gccatgttgg
     2041 gtcgtccggc tgaggcaatt gaagcttaca aagctgcctt acgtatcgat cgtcgaatag
     2101 aggaaatcta tctcttcatt gcgtttgagt ttcaaaatca aagtaaatac ggaaaagctg
     2161 cacgttatct acgtcgctgt ttaaagctga atccggagaa tgaatcggct ttgtatgaaa
     2221 tgtcgttttg tttcgatgtg accaataaac tcgaggctgg agtaaagtat ttcaattcat
     2281 tcctcgatga acatccatac tcgaaagtag cttggtatgc attaggtcat ttgcacagta
     2341 agcaacagca gtatattccg gcaattgaag catttgatta tgcaacagtt attgatgaaa
     2401 cctttgccag tgcttggttt aataagggga attgtcaggc tgctagtgag caatatgagc
     2461 aagcaattga gtcgtataaa gagatcttca aattagaaga acccgatgcg atgacttttt
     2521 tatacatcgg cgagtgttac gagaatttgg ataagtatcc cgaagcgtta aaatattatc
     2581 agcaggcttc ggccatggat gagtttttac ctgatgcatg gataggtatt ggagtggtac
     2641 tggatttctt agatcgaagt aatgaatcgc tttcctatgt gaagaaagga atcaacttag
     2701 atcctactcg tggtacattt tggtttattc taggtgattt gcagttgaag ttgggctttg
     2761 tgaatgaagc tgaagaatcg tacggaaaag tcctggaatt tgagcccgaa aatgaagata
     2821 tctgggtgtt tatgtgtaat ctactacttg aacaaggtcg ggttgatgaa gcgcaaacac
     2881 tcatgcaaga atgttttaaa taccatcccg gagatgcacg tttgactttt caatcgtgtg
     2941 aggtagaatt acataaagga aatttgcagg aagctttgat gttggctgaa atcgcattag
     3001 cgaccgatcc agctcttgga cctaaaatgc aagaagaatt accgcgcttt ggaatgaacg
     3061 cccaaattgc gcagcttgtc gaacgttaca gagatcaaga aaagaatgat taatatttta
     3121 ccttatttac ccgaacgcag cgctcaacct cgcgaaacgg gagttaccat gatgatggac
     3181 aaagggttga gtttccgtca ggcagaagat tttattgctc attcgggaca tttagctgat
     3241 gtaattaagt tgggatttgg aacctcattg gttgccaaga atgtgaaaga gaagattaaa
     3301 ttatatcagg atgctggtct gcgtgtttat gttggaggaa ctttatttga agcatttatc
     3361 attcgaaata aattcgatga ataccgcaaa tacattgata atcttggatt agatacgtgt
     3421 gaagtgtctg atggatcaat cgtattagat cacggtaaaa agtgtgatta tatctccaaa
     3481 ctagctaaaa actatcgtgt cctatcggaa gttggttcca aagaagcagg aattttaatt
     3541 gctcctgcca agtggatttc gatgatgaaa gctgagctgg atgctggatc ttggaaagta
     3601 attgcagaag cgcgcgaagc tggaaatgtg ggtatttatc gtcctaatgg aacagcacac
     3661 gttgtattgg taaatcgtat tttatccaaa attccttccg acaaaatttt gtgggaagcg
     3721 ccggttaaat ctcagcaagc ttggtttatt aaacatattg gagctaatgt aaacttgggg
     3781 aatatttcgc acgatgacgt aattccattg gaaacattga gattgggatt gcgtggcgat
     3841 acctttttcc aatttctacc agaagaactt cacaaactca aacaaaccgt ttaagcatgc
     3901 aggaaatatc cgttacagaa ttgaaaagct ggcgcgatca ggggaaagag catcaactta
     3961 ttgatgtccg cgaggattat gagtatgagg ctgctaatat tcaaggcgaa ctcattccta
     4021 tgggaactat tcctcaacat ttggattcga ttcgcagaga tattcctgtt gttctacaat
     4081 gcaaatctgg tgggcgttct ggtaacgtaa ctcgttatct tatgcaacaa ggatttacga
     4141 atgtatataa ccttacaggt ggaatttttg cttggattga acaagtagat ccatcgatga
     4201 gtaagtattg atacatgcgt cgatacggac ttttaggtcg aaaactggcc cataccttct
     4261 cccctgcata ctttgctcaa aaattcgagc aggaacaaat ccttgatgct caatacgaaa
     4321 catttgaact gaatggtttg gatgatttcc ctcgtttgct cactaaaaac ccaggtttga
     4381 aagggcttaa cgttactatt ccctacaaga ctgatatttg tgcttatcta gacgaacttt
     4441 ttggtccagc cgaagcatta caggttgtca atacagttgt ggtagaagaa gtcgaagagg
     4501 catttgaggg acaaaaaatt cccggttacc gacttaaagg atttaacacg gatatatatg
     4561 gatttaaaga atccatcaaa cctttgatgc gtccatggtt tgagcgtgcg ctgatattag
     4621 gaacaggagc tagtgcaact tctgtagcgt atgttttata taagttaggt gtggatacgt
     4681 tattcgttag tcgtaatcca gaaggtgaac aagaaatagg ttgggaagac atcaacgagc
     4741 atgtgattag atttcatccc ttgatcataa ataccacacc tattggtcag tttcctgata
     4801 tagatcaagc tcccgagtta ccctatcatt tattgacaaa tcagcactta gcgtatgatt
     4861 tggtatataa tcctgctgaa actttattct tgaagaaggc aaaagaagcc ggagcaatgg
     4921 tacataacgg acaatctatg ctcgaaatac aagcttcaca aagttggaag acttggcaga
     4981 atgcggagaa gcgataagat cagtttccta ggcgggcatc aacttgtacg ttgattttag
     5041 cacgcatttg aataggttca ggtaagattt gcaaatcgtt tcgaccgtaa aaactgaatt
     5101 ctaattcacc ttgtttaaaa tactccgcta aattaacatc agaaggagta atagtagtag
     5161 tggtttcggt aaaactatca ggtaattctt tggaggcaaa tatcacattc cctgcacctt
     5221 gattagataa acgtacttcg agttttttga agaaatcgag cgttttacct gatgtaataa
     5281 ctgcaatttg aatcgaactt actttaaccg atttgagatt gttaactgta aaaccgtttt
     5341 ccgacaggta attcaccaca tcggtttgaa ctgcttgaac acctaaaagc atatcaccag
     5401 ttgaagaagt tgtatcaacc gtgaaagtca tttcgggata ttcttttgta taactaagtg
     5461 gttcggccga ttttttacag gcagaaagaa aaagaaaggt accaacaatt gcactaagga
     5521 ttatccgagt attgagcaag tacataggaa tttttttacg aagttagaaa aactctattt
     5581 ctatccccta tttattcgat tatgcttttc gagctaatgc ttacttttga gcatgcttaa
     5641 attcggattg ctatttatat cggtgttgca aagtgcatgt ttactttggg cacagattcc
     5701 ttttcaatca ctcgctgatt ctcggttcta tttgaacagt aaaatgaccg tgcatggtta
     5761 tccggttcct gactattttg aagaaaaggt atttgattcc ttaataacat ttaagggaga
     5821 agccatgcat gaacaaaaat tcagtcgctt tacaacaaaa ggtcaatcgt tggaaacata
     5881 tcctcgcaag acctatttca gtgttaatgc aatccaagaa tggaagaaga atgaagtctg
     5941 gcattcgatt gacagaagtt tggaatctca aaaaggtgtg atttgcggga agaaaggaac
     6001 gatcaaacgt agtttcatcg aacaacagca tcctaaagtt g
//
LOCUS       DAOOQW010000144         6029 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_15957_length_6029_cov_4.460830, whole genome shotgun sequence.
ACCESSION   DAOOQW010000144 DAOOQW010000000
VERSION     DAOOQW010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 6029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6029
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15957_length_6029_cov_4.460830"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(13..390)
                     /locus_tag="PK281_08335"
     CDS             complement(13..390)
                     /locus_tag="PK281_08335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49394.1"
                     /translation="MKTLSGFLFLFLLGTQSMFGQTLSTQISTLYNDGVALIGEKKYA
                     EARLKLDQAVRLKSDYAEAIFARGTCSLMLNEREKSCIDFAMASKLGWKAADEYVTKY
                     CGADAIGRKVKPVEPKKAPGVSK"
     gene            complement(397..1218)
                     /locus_tag="PK281_08340"
     CDS             complement(397..1218)
                     /locus_tag="PK281_08340"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOY49395.1"
                     /translation="MRIQLRNTLLFTFLLSGTWAFSQTDAFRSESLELLKKGEAAKAL
                     EKMNKAVSIAPEQGENWLVLGKVHIAMNDRKTALKDFRKADSLKLATGDFYLNYGVAL
                     FDNGNSKEAFPLFEKGIKADPKNYELYYNRALAWIELERLDDAMNDLNTTIELKNDFT
                     DAYLQRAMLSFEDEHFKFCIKDCDQVLALNPNSSEGYFYRGMANGSSGNHAAAIKDFD
                     KALEMNPKNDEALCRRGAAKMLSGNKKGACIDWKKAVELGNDEAQQYLEVNCTAE"
     gene            complement(1228..1803)
                     /locus_tag="PK281_08345"
     CDS             complement(1228..1803)
                     /locus_tag="PK281_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49396.1"
                     /translation="MKSVFCVLLVSVLSLLSCTAPDTVQLSGYQGTPEQGLFASIPIC
                     YSTLPIALGDTLSEVREKTQVYEDLNPTNLEEYSTLEWTEHHAENGTDSVIRCTFKNQ
                     RLVHFSSDTYLDELCPTKTELISRVDPMFTCMSEVRPILYFGKNLSFTQVDDALAQHY
                     QILKQDSGYKGLKYEIGYTEDSMKVTIAAHS"
     gene            complement(1879..2400)
                     /locus_tag="PK281_08350"
     CDS             complement(1879..2400)
                     /locus_tag="PK281_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49397.1"
                     /translation="MEFRLTSVLIGLLVSMSCYAQVFPPVNCSNLNGQTVNLPTSKEK
                     YAVLLVTFGKKGNEAAETWVDPLVQKFIRKSGLLDAFFTADVYALSIVNSAEWAMIRS
                     QDQKIKADIPSELISNLVYSQTNPQPILDVLGEKAKNTATVLLIQSDGKIIRIVSGDY
                     SDKKLEELEEGFE"
     gene            complement(2436..3599)
                     /locus_tag="PK281_08355"
     CDS             complement(2436..3599)
                     /locus_tag="PK281_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009410265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HOY49398.1"
                     /translation="MVSFENTEIAFKSKSNQELSKAWWLFRFIGSNQLVRIAKPFAGI
                     AVNIGFPFRYMIKQTIFQHFCGGENIGDCEHTIAHLAKYHVHTILDYSVEGKESEADF
                     DRTTQEIIDTQERSVLDEHIPFSVFKLTGIARFAILEKASSGETLTEAERSELDRIRH
                     RVERICKRASELERSVMMDAEESWIQPIMDELMLEMMKRFNKEKAVVYNTFQMYRHDR
                     LAKLKELHAQALEFGFYPGVKLVRGAYMEKERARALEMSYPSPIQPDKNATDRDYNAA
                     LTFMMEHIDRFSICAGSHNESSAALLIQLMEEKKIPKNDQRVYFSQLLGMSDHISFNL
                     SHAGYNVAKYVPYGPVKDVLPYLIRRADENTSVAGQTSRELSLLNKERTRRKK"
     gene            3696..4778
                     /locus_tag="PK281_08360"
     CDS             3696..4778
                     /locus_tag="PK281_08360"
                     /EC_number="4.2.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase family protein"
                     /protein_id="HOY49399.1"
                     /translation="MNKEPISTNPYIECGTDTLQSCSILVNKIQPDRIFLLCDSNTHR
                     DCLPIFLQQNPDLSPQILVLEQTGEHSKTLETVSWLCESLLKNGASRKSLLIVLGGGI
                     ACDLGGFAASIFMRGMSFMHIPTSLLAMVDASSGGKTGVNFLGNKNMLGVFQQASAVL
                     IYPPFIQTLTETELISGFAEVIKHALLSGDEQWEFIQQIHPLKYSQWDELIRENVLFK
                     KRITDLDFQEKGVREQLNLGHTFGHAFEAFSESKNVHFPHGFAVASGILAELILSKNT
                     GFLKHPELIEEFNQYYRRYFHPFLFGESDIPALIEFMKVDKKNTSGQITFCLLETPGI
                     VHYQQAPAEDQILIALHQYLVYARRS"
     gene            4762..6006
                     /locus_tag="PK281_08365"
     CDS             4762..6006
                     /locus_tag="PK281_08365"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HOY49400.1"
                     /translation="MHDVLELVSSTRRINGSVQLPASKSISNRLLILEEISSGIVNGI
                     GYSDAEDTQILQQLLHDKSNLAHAGAGGTTFRFLLAVLASTPGYEGVLEGSKRLMERP
                     VAPLVDALLLAGADIEYVDKPGYAPLRIRGKQLHGGKINVDASLSSQFITALLLIAPR
                     MSAALELIPQGDLISAPYIHMTVSLMKQCGFQVLQTDSSILVQPFIPIHKIQIPVERD
                     WSAASYWYACVALSEKASLQLPGLTIQSLQGDSACQEQFKKLGVLGVETADGIELQKI
                     LIEKTDLDFNCLTTPDLFQTFAVTVAGLGLKASFTGLQSLPHKETNRIEAVSKELSKI
                     GIRCTFDSNGAFKLDASQIDPNRVLIAETYEDHRMAMAFSPLVLVLKKIAIRNPDVVR
                     KSYPGFWADIRRIGISFAEWNG"
BASE COUNT         1771 a         1240 c         1110 g         1908 t
ORIGIN      
        1 gaaaaacaaa atttacttag atacacccgg tgctttttta ggttctacag gctttacttt
       61 tcgtccgatt gcatcggctc cacagtattt cgtcacatat tcatcagcag ctttccaacc
      121 tagtttagaa gccattgcaa agtcgataca cgatttttcc cgctcattta acattaaact
      181 acaggtacca cgtgcaaaaa tagcttctgc ataatcgctt tttaaacgaa ctgcttgatc
      241 taatttcaat cgtgcttccg catatttctt ctcaccaatt aatgccactc catcattgta
      301 aagggtgctt atttgcgttg aaagtgtttg cccaaacatc gactgagtgc caagaaggaa
      361 cagaaacaga aatccagata gtgttttcat agtttgttat tcagcagtac aatttacttc
      421 tagatattgt tgagcctcat catttcccaa ttctacagcc tttttccaat cgatacaggc
      481 tcctttttta tttcccgata acattttagc tgcaccacga cgacatagag cttcgtcatt
      541 cttagggttc atctctaatg ctttatcgaa atctttaatt gcagcagcat gattaccgct
      601 acttccattt gccattccac gatagaaata tccttcagaa ctattcggat ttaaggctaa
      661 aacttggtca caatctttaa tacaaaactt gaagtgctca tcttcaaagc taagcattgc
      721 tcgttgaaga tatgcgtcag taaagtcatt ttttaattca attgttgtat tcaagtcatt
      781 catggcatca tctagccttt caagctcaat ccaagctagt gctctattgt aatacaactc
      841 ataattttta ggatcagctt ttattccttt ttcaaaaagt ggaaaagctt cttttgaatt
      901 cccattgtcg aaaagtgcta ctccataatt taaataaaaa tcacctgtcg ctaatttcaa
      961 agaatcagct ttacggaagt ctttaagcgc tgtttttcga tcattcatgg ctatatgcac
     1021 tttaccaaga actaaccagt tttcaccttg ttcaggagca atactaacag ctttgttcat
     1081 tttttctaat gctttagctg cttctccttt tttcaataat tccaaacttt cggaacgaaa
     1141 tgcatcagtt tgtgaaaatg cccatgttcc acttaaaaga aaggtgaaaa gtaatgtgtt
     1201 gcgtaattgt attctcatgt gttaggatta agaatgtgcg gcaattgtca ctttcatact
     1261 gtcttcggta taaccaatct catatttgag tcctttatac cccgaatctt gttttagtat
     1321 ttgataatgt tgtgcaagtg catcatccac ttgtgtaaaa ctcaaatttt taccgaaata
     1381 taaaattggt cttacttccg acatgcacgt aaacatagga tcaacccgac ttatgagctc
     1441 tgtttttgtt gggcatagct catccaaata agtatcacta ctaaaatgaa ccaatcgttg
     1501 attcttgaat gtgcaacgta ttactgaatc ggtcccattt tcagcatgat gctctgtcca
     1561 ctctagggtt gaatattctt ccaagttagt aggattcaaa tcctcataaa cctgtgtttt
     1621 ttcacgaacc tcgctcaagg tatcacccaa tgctattgga agagttgaat agcaaatagg
     1681 aatagaggca aacaagcctt gttcaggagt gccctgataa ccagataatt gaaccgtatc
     1741 aggagcggtg caggaaagaa gcgaaagcac acttactagt aaaacacaga atactgattt
     1801 catgcttgag aattaatcac ccaaacctac aaaaaacagg gtttaaatgg aaacaatacg
     1861 aaagagtgtc gctttatttt attcaaatcc ttcttccagt tcttccagtt ttttgtcgga
     1921 ataatctcca gaaactattc gaatgatttt tccatccgat tgaatcaata aaacggtggc
     1981 tgtattctta gctttttcac ctaagacatc cagaattggc tgaggattcg tttgggagta
     2041 aaccaaattc gaaataagtt cagaaggaat atctgcctta attttctgat cctgagaacg
     2101 tatcatcgcc cattctgcac tattcacaat tgaaagggca tacacatcag ccgtaaaaaa
     2161 agcatccaac aatccacttt tacgtataaa tttctgaacc aaaggatcta cccaagtttc
     2221 ggcagcttca tttccctttt tgccaaatgt gacaagtaaa acagcatatt tttccttgga
     2281 tgtaggaagg ttaactgttt gaccatttaa attactgcaa ttaactggcg gaaaaacctg
     2341 agcataacag ctcatgctta ccagtaatcc tattaacaca cttgtcaaac gaaattccat
     2401 ctcccaattt tcaagctaac gaataagtca gctacttatt ttttcctgcg tgtgcgctct
     2461 ttgttgagta aacttaattc acgactcgtt tgtccggcaa cggaagtgtt ttcatccgcc
     2521 cttcgaatga ggtatggaag tacatctttt acagggccat acggaacata tttagctaca
     2581 ttataaccag catgagaaag gttaaagcta atgtggtcgc tcattccaag cagttgggag
     2641 aaatatacac gctgatcgtt tttaggaatt ttcttttctt ccatcaattg gataaggaga
     2701 gcagcgctac tttcattatg cgaaccggca caaatcgaaa aacgatcgat atgctccatc
     2761 ataaacgtta atgctgcgtt gtaatcgcgg tcggtagcgt ttttgtcggg ttgaattggt
     2821 gatggatagc tcatttccaa agcacgagca cgttcttttt ccatgtaagc accacgaaca
     2881 agcttcactc cagggtaaaa tccaaattcc aatgcttgag catgtaactc ttttaacttg
     2941 gctaaacgat cgtgacgata catttggaag gtgttgtaaa caacagcttt ttctttgttg
     3001 aaacgcttca tcatttccag catcaattca tccataatag gctgaatcca gctttcttct
     3061 gcatccatca tcacgctacg ttcgagttct gaagcacgtt tgcagatgcg ttcaactcgg
     3121 tgacgaattc ggtcaagttc gctacgttct gcttcagtta aagtctctcc ggagctagct
     3181 ttttctaaaa tagcgaaacg tgcaatacca gtgagtttga ataccgaaaa tggtatgtgt
     3241 tcatctaaga cagaacgttc ctgcgtatca ataatttcct gtgtcgttcg atcaaaatca
     3301 gcttccgatt cttttccttc taccgaataa tcgagaatgg tatggacgtg atatttagcc
     3361 agatgggcaa ttgtatgctc acagtcaccg atgttttctc caccacaaaa atgctggaaa
     3421 atggtttgtt taatcatgta tcggaatgga aatccgatat tcacagctat tcctgcaaaa
     3481 ggttttgcta ttcgaactaa ttggttgcta ccgataaacc gaaacaacca ccaggcttta
     3541 cttaattcct gattgctttt actcttaaaa gcgatttctg tattttcaaa ggacaccatg
     3601 cggcgcagat tatttggcaa acctaacaga atttatcaaa ccagatatag cttcttctaa
     3661 cgttcttcca accggattat actactttta cccacatgaa taaggagcca atttcgacca
     3721 atccatacat cgaatgtgga acagacacgc tccaatcatg ttcaattctg gttaataaaa
     3781 ttcagccgga tcgtatattt ctgctttgcg actcaaatac gcatcgcgat tgtttgccca
     3841 tttttcttca acagaatcct gatttatctc cacaaattct tgtgctcgaa caaacaggag
     3901 aacattccaa aacacttgaa acggtttcat ggctgtgcga atcacttttg aaaaacggag
     3961 ctagtcgaaa aagtttgttg attgttttag gaggtggaat tgcctgtgat ttaggtggat
     4021 ttgccgcttc cattttcatg cgtggtatgt cgttcatgca catcccaact tctttgcttg
     4081 ccatggtaga tgcctcttcg ggtggcaaaa cgggagtgaa ttttttgggg aataagaaca
     4141 tgctcggtgt ttttcaacaa gcatcagcag tgctcattta tccacctttc attcaaactt
     4201 taaccgaaac ggaattaatc tcaggatttg ccgaggtcat taaacatgct ttgctttcgg
     4261 gcgatgagca atgggagttc attcaacaga ttcatccgct taagtattcc caatgggatg
     4321 aattaattcg ggagaatgtg ttgtttaaga agcgaatcac ggatttagat ttccaagaaa
     4381 aaggtgttcg tgaacagctc aatttgggac atacattcgg acatgcattt gaggcctttt
     4441 cggaaagtaa gaatgttcat ttccctcacg gatttgctgt tgcctccgga atattagctg
     4501 aattaatttt atcgaagaat actggctttt taaaacatcc ggaattaatt gaagagttta
     4561 atcagtatta ccgacgatat ttccaccctt ttttgttcgg tgaatcggat attcccgcac
     4621 ttattgagtt catgaaagtg gacaagaaaa acacatccgg acagattacc ttttgccttt
     4681 tagaaactcc cggaattgtt cattatcagc aagctccagc tgaagatcaa attctaattg
     4741 cgcttcatca gtacctagtt tatgcacgac gttcttgagt tagtttccag tacccgacgt
     4801 ataaacggaa gtgttcaatt gcctgcatcc aaaagcatca gcaatcgctt attaattttg
     4861 gaggaaatat cttctggaat cgttaatgga attggatact cggatgcaga agacacgcag
     4921 attcttcaac aacttttaca cgataaatct aatctagcac atgccggtgc aggtggaacg
     4981 acttttcgtt ttttactagc agtattggca tcaactccag gctatgaagg tgttttagaa
     5041 ggttctaaac gtttaatgga acgtccggtt gcccctttag tagatgcttt gctattagct
     5101 ggagctgata ttgaatatgt agataaaccg ggatatgctc cacttcgaat tcgtggaaaa
     5161 cagctacatg gcggaaaaat aaacgttgac gcatctctta gcagtcaatt cataacggca
     5221 cttttattaa ttgcacctcg tatgtcggct gctttggagt taattcctca aggtgatttg
     5281 atttctgctc catacataca tatgactgtt tctttgatga aacaatgtgg ttttcaggtg
     5341 ctgcaaacag actcttctat tcttgttcaa cccttcattc caattcataa aatccagatt
     5401 ccagttgaac gagattggtc ggcagcttct tattggtatg catgtgtagc cttgtcagaa
     5461 aaagccagtt tacagcttcc cggacttact attcaatccc ttcaaggcga ttctgcttgt
     5521 caggaacaat ttaaaaagtt gggagtttta ggagtcgaaa cagccgatgg gattgaattg
     5581 cagaagattc ttatagaaaa aacagacctc gattttaatt gtctaacgac acccgattta
     5641 tttcaaacct tcgctgtaac agtagctgga ttagggctta aagcaagctt tacaggctta
     5701 caatccttac ctcataaaga aaccaatcga atagaagctg tttcaaaaga gttatcaaag
     5761 attggaataa gatgtacgtt cgatagtaat ggagcattta agttagacgc ttcgcaaatc
     5821 gatcccaatc gggttttaat tgcagaaacc tatgaggatc atcgtatggc tatggcgttt
     5881 tctccattag tactagtatt aaaaaaaatt gccattcgaa atccagatgt agtaaggaaa
     5941 tcttatccag gtttttgggc cgatatcaga agaatcggga tttcatttgc cgaatggaac
     6001 gggtgaaaat atttacagcc taataataa
//
LOCUS       DAOOQW010000145         6007 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_16019_length_6007_cov_4.368616, whole genome shotgun sequence.
ACCESSION   DAOOQW010000145 DAOOQW010000000
VERSION     DAOOQW010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 6007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6007
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16019_length_6007_cov_4.368616"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..663)
                     /locus_tag="PK281_08370"
                     /pseudo
     CDS             complement(64..663)
                     /locus_tag="PK281_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803900.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase"
     gene            complement(829..1251)
                     /locus_tag="PK281_08375"
     CDS             complement(829..1251)
                     /locus_tag="PK281_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49401.1"
                     /translation="MISVLGTTSKYILQPALIDMHRQSTEWLSVTELWKQELSFYQKL
                     LDQHNATNTNVEFKKQVDHFQNLITYYNGELIITFQKKIREHERKLARMLRELNESDT
                     EYFKEHNGLMEELDSFQQNYLLFKKTFYDFIEKGFSAY"
     gene            1403..1852
                     /locus_tag="PK281_08380"
     CDS             1403..1852
                     /locus_tag="PK281_08380"
                     /inference="COORDINATES: protein motif:HMM:NF024304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOY49402.1"
                     /translation="MIGTLNKLQIDFLLRSELLGRIGCSADGITYVIPITYVYDGKYI
                     LAHTREGTKIEMMRKNANVCFEVDRIQDMANWQSVIIQGKYEELSGKKAEDTVQLLVN
                     RMHPFASSETMVPRYALAQHKTPLAQDSRMIVFRIRIVEATGRFEKK"
     gene            1997..2326
                     /locus_tag="PK281_08385"
     CDS             1997..2326
                     /locus_tag="PK281_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002979942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HOY49403.1"
                     /translation="MNWLTLIIAGFCEIAFAACLGKAKESTGTTAAMWFGGFFVFLSL
                     SMYLLYKATQTLPIGTAYAVWTGIGAAGTALIGIFIFKEPADFWRVFFIITLIASIVG
                     LKTVSST"
     gene            2323..2715
                     /locus_tag="PK281_08390"
     CDS             2323..2715
                     /locus_tag="PK281_08390"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOY49404.1"
                     /translation="MKNLIIILAIALTACSTSSRNENSVNASRGIEKLDVATFQSKVK
                     ESSNAVVLDVRTAEEFKTGNIEGAINIDFKASDFEQKIANLDKSKTYFVYCLSGIRSA
                     KAADRMSELGFTSLYTLDGGYRALTENQ"
     gene            complement(2751..3410)
                     /locus_tag="PK281_08395"
     CDS             complement(2751..3410)
                     /locus_tag="PK281_08395"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOY49405.1"
                     /translation="MKLQTSNSNTISPIQIITQDLGKRFNREWIFRNLTFNFTTGNTY
                     AITGPNGSGKSTLLHVLWGQVPASIGTIRYTQGDVEIEAEQIHQHISIATPYMDLVDE
                     FTLIEQLQFHFKLKKSRDGMSLGNMLDRMNLAHAKDKQISNFSSGMKQRLKLALAFFT
                     EAEIIFLDEPGTNLDSQAFAWYLKELNALPKTTLIFIASNQPAEYPENAVVLNISDYK
                     K"
     gene            complement(3518..4306)
                     /gene="lpxA"
                     /locus_tag="PK281_08400"
     CDS             complement(3518..4306)
                     /gene="lpxA"
                     /locus_tag="PK281_08400"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007649251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HOY49406.1"
                     /translation="MSRFPLANVHPDAKIGNNVTIEPFATVQADVTIGDECWIGPNAI
                     LWDGTRLGKNVKIYPGASVSSIPQDLKFSGEKTETYIGDNTVVREYVTISRGTTDKFK
                     TVIGKNCLLMAYVHVAHDCNIGNHCILVNTVQVAGHVTIDDWAIIGGASALHQFVKIG
                     AHVMISGGSLVRKDVPPYTKAAREPLAYAGINTVGLRRRGFAAEKISDIQEIYRYIFL
                     KGLNNSKALDIVESEIAKSEEKDYILNFIKTSDRGIMKGYGSKD"
     gene            complement(4303..5700)
                     /locus_tag="PK281_08405"
     CDS             complement(4303..5700)
                     /locus_tag="PK281_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008614374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase/3-hydroxyacyl-ACP
                     dehydratase"
                     /protein_id="HOY49407.1"
                     /translation="MLNIKQQTIQKSVTLSGVGLHTGVQTNMTFVPAKPNHGIKFQRI
                     DLPGSPIIDADCDNVVDVSRGTTIEQSGARVSTIEHTLAALTGLEIDNVLIQLDGPEC
                     AIMDGSSIQFVDILKEAEPVEQNAIRDFFEVQEPLFYRETARNVEIAALPLDDYRVTV
                     MVDYNSPVLGSQHASITDIRQFEKEIASCRTFCFLHELEMLYKNNLIRGGDLNNAIVI
                     VDRVVQEDELDNIAKMLGKPKVAVKQEGILNNIELRYKNEPARHKLLDIVGDLTLAGR
                     PLKAQILAARPGHAANVAFAKKLKKAMADSDRKGRPKYNPSLPPVMDINKISGILKHR
                     YPFLMIDKVIYLDNESVAAVKNVTVNEPYFQGHFPGNPIMPGVLQVEAMVQTGGILVL
                     NTVPDPENYWTYFIGIENFRFRKMVLPGDTLVIQCDLLAPIKRGIAKMSGRAFVGNTL
                     VCEGTMIASIVRKDL"
BASE COUNT         1673 a         1196 c         1269 g         1869 t
ORIGIN      
        1 taccttttag gtagaagtgt ccagaatctg atcgggttgc cgctcttctc tcggtcgact
       61 gctgcccgga tcttttacat agagctcttc agcaaagttt ccatctgaat gtaaatgggt
      121 agagagaatc cccgctttat cggaaggttg gaggattgct gcaccggcac catcggcaaa
      181 aataacggcc gtatttcttc cccgggttgt ggcatctatt gcggttgatt gaatttcggc
      241 accaacaacc aatatggttt tatacatacc ggttttaata aattgatctg ccaccgaaag
      301 tgcatagata aatcccgaac aagcattccg gatgtcaagc gcccctattc ttccatccag
      361 gccaagttcg cgttgaagca aaacaccaga gccagggaag aaataatcgg gagtaatggt
      421 ggcgaatacg ataaagtcga tatctttttc agtgagcttt gcgcgttcaa gcgccatgcg
      481 gctagctttt gcggccatgt ttgccacggt atccttggcg ggatcaatcc acctgcgttc
      541 atgaatacct gtacgttcaa cgatccagtc attgtttgta tccatgatgg ttgaaaggta
      601 ttcattggtg atgacagttt caggaacatg gtgcccaaga cccattattt tagaatggcg
      661 cattcgattg aatttatgga atgctaagat aaagaatagt ttccgaatgg ctgttagtaa
      721 aaaaataagg actgttttaa agctgtgatc ttctctattg taattcgggt tgaagaattc
      781 aagagatcga agatttaaaa caatcctttg tctggcttga tactgaaatc aatatgcact
      841 gaatcctttt tctataaagt cgtaaaaggt cttcttgaat agaaggtaat tctgttggaa
      901 agaatcgagt tcttccatca gtccattatg ctccttaaaa tattctgtat ccgattcgtt
      961 taattcccgg agcatgcgtg cgagtttacg ttcgtgctct cttatttttt tttgaaatgt
     1021 gatgatgagt tcaccgttgt agtacgtaat gaggttttga aaatgatcta cttgtttttt
     1081 aaactcaaca ttggtgttgg tagcgttgtg ttggtcaaga agcttttgat aaaaggataa
     1141 ttcctgtttc caaagttcgg ttacggacaa ccattctgtt gactgccgat gcatatcaat
     1201 caatgccggt tgtaaaatgt attttgaggt agttcctagt acggagatca tggtgttgtg
     1261 tgttttgttt tactttatat tttgaagtac gttcctttta gtttgaaaac ttctgagata
     1321 agtcagtcta cctacctgat tgttatcatt ttcctttttg gattaaggcg ctaacttttt
     1381 attcaaaata atcctatttg ttatgattgg aacattgaat aaacttcaaa ttgatttcct
     1441 tttgcgaagc gagttgctcg gtcgcattgg atgttcggct gatggcatta cgtatgttat
     1501 tccaataaca tacgtttatg atggtaagta cattctggca catacgcgag aaggaacgaa
     1561 gattgagatg atgcgaaaaa atgcaaatgt ttgctttgaa gtggatcgta ttcaggacat
     1621 ggcgaattgg caaagcgtca ttatacaagg taagtatgag gaattgagtg gaaagaaggc
     1681 agaagataca gttcaattat tggttaaccg tatgcatcca ttcgcaagca gcgaaaccat
     1741 ggttcctcgt tatgctctgg ctcagcataa aacgccttta gctcaggatt cccggatgat
     1801 cgtatttcgg attcgaatag ttgaagccac gggtagattt gagaaaaagt aaaaataagg
     1861 tagcgttcaa attggattgt taccgtgtta cttttcttcc acttcgcatt gattccgttt
     1921 ccaaaaaccg aatcaatttg gatgcgatgc tgattttact tctttatcca gacaaaccta
     1981 tatagcttta ctgaatatga attggctgac cctcatcatc gctggatttt gtgaaatagc
     2041 atttgctgct tgcctgggaa aagcaaagga atctacagga acaactgcag caatgtggtt
     2101 tggcggattt ttcgttttcc tctcactaag tatgtatttg ctttacaaag cgactcaaac
     2161 attaccgata ggtacagcct atgctgtatg gacaggtatt ggcgcagcgg gtacagcatt
     2221 gattggtatc tttatattta aagaaccagc cgatttctgg agggtatttt ttattattac
     2281 acttatcgca tctatcgtag gacttaaaac agtttcaagc acatgaaaaa tcttatcata
     2341 attttagcaa ttgccttgac agcatgttca acgtcaagcc gaaatgaaaa ttcggtaaac
     2401 gcatcaaggg gaattgaaaa gttagatgtt gctacatttc aatctaaagt aaaggaatct
     2461 tctaatgctg ttgtattgga tgttcgcacc gcagaagaat ttaagacagg taacatagaa
     2521 ggcgcaatca acattgattt taaggcctct gattttgaac aaaaaattgc aaaccttgat
     2581 aaaagcaaaa cctattttgt gtattgcctc agcggaatca gaagtgcaaa agccgcagat
     2641 cgaatgagtg aactgggctt cacttcactg tatacgcttg atggtgggta cagagcattg
     2701 acggagaatc aataaaccga ttaccagtca ctaattacca cttaccgttt tcacttcttg
     2761 taatctgaaa tattcaacac aacagcattc tccggatatt cagcaggctg attgctggca
     2821 atgaaaatga gcgttgtttt tggtagtgca tttaattctt taagatacca tgcaaaggcc
     2881 tgactgtcca gattagtgcc gggttcatcc agaaaaataa tttcggcttc agtaaaaaat
     2941 gctaatgcca gttttagccg ctgtttcatc ccggaagaga aattcgatat ctgtttatct
     3001 ttagcatgag cgagattcat tcgatcgagc atatttccta agctcattcc atcgcgactc
     3061 ttttttaact tgaaatgaaa ttgtagctgt tcaattaaag taaactcatc aaccaaatcc
     3121 atgtacggag tggcaatgct gatgtgctga tgaatttgtt ctgcttctat ttcaacatcg
     3181 ccttgagtgt aacgaattgt tcctatgctt gctggtactt gcccccaaag aacatgcaat
     3241 aaggtagact taccagatcc attcggacct gtaattgcat aggtatttcc agtagtaaaa
     3301 ttaaaagtca ggtttcggaa tatccattcg cgattgaaac gctttccgag atcttgtgtg
     3361 atgatttgaa tgggagaaat ggtattggag tttgatgttt gaagtttcaa attcagttaa
     3421 tcgttatccg ttaatcgtta atggtcgtaa ggtttgacgt ttcctatttc gaaaagagat
     3481 ccacgattaa cgtttaacca ttaacggata acgatcctca gtccttcgat ccgtaaccct
     3541 tcataatacc acgatccgaa gtctttatga aattaagaat gtaatctttc tcttcgcttt
     3601 tcgcgatttc gctctctaca atatcaagcg cttttgagtt gttcaggcct ttcagaaaaa
     3661 tgtatcggta tatttcctga atgtcgctga tcttctctgc tgcgaaacca cgtctcctca
     3721 aacctacagt attgattccg gcatacgcca atggttcacg tgctgctttg gtgtaaggtg
     3781 gtacatcttt gcgaaccagt gatcctccgg agatcattac gtgcgctcca atttttacaa
     3841 actgatgcaa cgcactggct cctccaataa tagcccaatc gtcaatcgtt acgtgaccgg
     3901 caacctgaac tgtatttact aagatgcaat gattaccgat gttacaatcg tgcgctacgt
     3961 gaacgtaggc catcaacaaa caattctttc cgataacggt tttgaattta tcggtagtac
     4021 cgcggctaat ggttacatat tcacgcacaa ccgtgttatc accaatatag gtttctgtct
     4081 tttcgccaga gaattttaaa tcttgtggaa tagaagaaac agaagcaccc ggataaattt
     4141 ttacattttt tcctaaacgt gtaccatccc atagaatggc atttggtccg atccaacatt
     4201 catcaccaat ggtaacatcg gcttgaacgg tagcaaaggg ttcgatggta acgttgttac
     4261 ctatctttgc atcggggtgt acgttggcta gagggaatct actcataagt ctttgcgaac
     4321 aatgctcgcg atcatagtac cttcacatac aagtgtatta ccaacaaagg ctcttccact
     4381 catttttgct atacctcttt ttataggggc taagagatcg cattgaatga ccaatgtatc
     4441 gcccggtaat accatttttc taaatctgaa attttcgatt ccgataaagt aggtccaata
     4501 attttcagga tctggaacgg tattcaaaac caaaattcca cccgtctgta ccatagcttc
     4561 tacttgcaaa acacccggca taattgggtt tcctggaaaa tgtccttgaa agtaaggctc
     4621 attaaccgtt acgtttttta ccgctgctac actttcattg tcaaggtaga taaccttatc
     4681 aatcatcagg aaaggatagc ggtgtttcag aatgcctgag atcttgttga tgtccattac
     4741 cggaggaaga ctcggattgt atttcggtct gcccttcctg tcggaatccg ccatggcttt
     4801 ctttaacttt ttagcgaaag cgacattggc tgcatgccca ggacgagcag ctaatatctg
     4861 cgccttcaac ggacgaccgg ctaaggtaag gtcgcctaca atatccaata gtttatgacg
     4921 tgctggttcg tttttataac gaagctcgat gttgttcaaa ataccttcct gctttacggc
     4981 aactttaggt ttgccaagca tctttgcaat gttgtcgagt tcatcttctt gtacaacccg
     5041 atccacaatt acaatagcgt tattcaaatc gcccccgcgg atcaaattgt ttttatagag
     5101 catctccaat tcgtggagga agcagaaggt gcggcacgaa gcaatttctt tttcaaattg
     5161 acggatgtca gtgatggaag catgttgact tcctaacaca ggtgaattat aatccaccat
     5221 taccgtaacc cgatagtcat ctaaaggcaa agctgcaatc tctacattgc gggctgtctc
     5281 tctatagaaa agaggttctt gcacctcgaa gaaatcgcga attgcatttt gttctactgg
     5341 ttcagcttct tttagaatat caacaaactg aattgagctc ccatccataa tggcacattc
     5401 agggccatcg agttggatca gaacattgtc aatttctaat ccggttaggg cagccagcgt
     5461 atgttcgatg gtgcttaccc gtgcgccact ttgttcgatg gtggttccgc gcgaaacatc
     5521 cacgacatta tcgcagtcgg catcaattat gggagagcct ggaagatcga tccgctggaa
     5581 tttgatcccg tgattcggct tagccggaac gaaggtcata ttggtttgaa ctccagtgtg
     5641 aaggcctacc cccgagagcg ttacggactt ctgaatagtt tgctgtttga tattgagcat
     5701 gttttacgaa agtgggcaaa tttaacccga ataatcggga ttatccaaaa atgaccgttt
     5761 tgggctgaat agtggattgt tgagggtaaa tggtcgttgt aaggaacttg ccaccagtac
     5821 ctgcttagga gatcaagatg cacaagattc taggagaaac cgtgcttcca gttttgaaat
     5881 atgttttaat agcctgattt taggtcttgg aacatctaca aacagttgcg ggtaatctga
     5941 aactccgtct attaaggaat atatcagaca gtctaatatc gggatgttat tacgcatttg
     6001 tttaggt
//
LOCUS       DAOOQW010000146         5985 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_16098_length_5985_cov_5.266610, whole genome shotgun sequence.
ACCESSION   DAOOQW010000146 DAOOQW010000000
VERSION     DAOOQW010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5985
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16098_length_5985_cov_5.266610"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /gene="nbaC"
                     /locus_tag="PK281_08410"
     CDS             complement(<1..420)
                     /gene="nbaC"
                     /locus_tag="PK281_08410"
                     /EC_number="1.13.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015482278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyanthranilate 3,4-dioxygenase"
                     /protein_id="HOY49408.1"
                     /translation="MPIQRPFSFKKWIDENRHLLKPPVGNQVVYKTADDIIVMVVGGP
                     NKRKDYHYNESEEFYFQLEGNMTLKIIEDGKPVDIHINEGDIFMLPGRTPHSPQRPEN
                     TVGLVVELVRPKQENDAFMWFCESCGNKLYEEFLHVSD"
     gene            465..1253
                     /locus_tag="PK281_08415"
     CDS             465..1253
                     /locus_tag="PK281_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007650617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOY49409.1"
                     /translation="MELNLAGKTALVCGSTQGIGRACARELAEAGAHIVLLARDETRL
                     KEEKIELDRINHLNNDYLVADFNDWKQVESVVKAFVAKSNPIHILVNNNGGPAAGPIT
                     DARPEAFEAAFTQHLLCNQVLVQAVVNEMIKADYGRIINIISTSVKAPLHGLGVSNTI
                     RAAVGNWSKTMANELGKYGITVNNVLPGATMTGRLSQIITNKAARTHQEVAHVEEEML
                     GEIPLKRFAKPEEIAHAVTFLASPAAAYINGINLPVDGGRTASL"
     gene            1497..1928
                     /locus_tag="PK281_08420"
     CDS             1497..1928
                     /locus_tag="PK281_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49410.1"
                     /translation="MKLVRHFLIGLIILVSHEINAQGIEKTFRGQWASSFWTFEFHSN
                     NNYKRTSSGHYGHTIVEGTYRFYNDTLEILNGYEESNGTINRYYLLDGNRIIDLEILY
                     DYYAEEGFPISWKNKRILVNQTKGMPPKYWYEPEILKYIRD"
     gene            1957..2580
                     /locus_tag="PK281_08425"
     CDS             1957..2580
                     /locus_tag="PK281_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49411.1"
                     /translation="MKKHLLISLLLCLVYAGSFGQTRHGDSAWYEGRYRSQEEMNDIR
                     KKEAKEKFESWFMPGLGYSMYQPKKRDSTGLFSGLTVEYLIYANVDQNDDPGPSHVRV
                     YSKLNILNSDRKSINSMFMYTIGLDLSLEKNPKRQFLIPYFGVEMGGISQKQFGSGIQ
                     FTPTFGLHLLSKKNMFINIHGGYVYPIRNFEMLQGWYGQAGLNFALW"
     gene            complement(2599..3018)
                     /locus_tag="PK281_08430"
     CDS             complement(2599..3018)
                     /locus_tag="PK281_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49412.1"
                     /translation="MKPNSLIALNLRIEELEAIRAIQHKELGFQIQSVMDNLKPFNII
                     KTAIHDVMELPNMKGGIGNLAIGSITGILAKKILWGNSINPVRSILGIITQTLVTNVA
                     TNNAEDIRDKGSNLIQPLIQRFFQYRKMKRAELENNN"
     gene            complement(3018..3377)
                     /locus_tag="PK281_08435"
     CDS             complement(3018..3377)
                     /locus_tag="PK281_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49413.1"
                     /translation="MHIQMEDQATIMESILEKGELYTQTNIELIRLKTIGKSAEVFSS
                     LAGGFVIFVSVLIVLMMINIGAAIWIGSLFGVSYYGFFIVACFYLLITIIFLAFKNVL
                     IKMPISNLIINNLYKLN"
     gene            complement(3377..3877)
                     /locus_tag="PK281_08440"
     CDS             complement(3377..3877)
                     /locus_tag="PK281_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49414.1"
                     /translation="MKLINVSVLSLLFSVSLFTISCTKKEGCTDIQADNYDSEADENT
                     GCQYRYVTTIDISNVPTANPAGTEWDIDGSGPELKLNFGKNTSSGYDYTTNSVSDVFA
                     TSLTPNSNIQFTNETWKYQLVDEDLLSSDEVIASGTFNPISQGSNSIITISNGSISLT
                     FHYKLQ"
     gene            complement(3889..4203)
                     /locus_tag="PK281_08445"
     CDS             complement(3889..4203)
                     /locus_tag="PK281_08445"
                     /inference="COORDINATES: protein motif:HMM:NF024143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HOY49415.1"
                     /translation="MNSGKVLLGVLAGVAAGAFLGILFAPDKGANTRKKIISKGDDFA
                     DGLKEKFNEFLNQMEDKYSEVKDNAEELVESGKAKFQDAKMDLKNQVNEKVGQLAQNN
                     NH"
     gene            complement(4597..5547)
                     /locus_tag="PK281_08450"
     CDS             complement(4597..5547)
                     /locus_tag="PK281_08450"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOY49416.1"
                     /translation="MAIIGTDIHQAAQLLQNEVIGIPTETVYGLAGNAYNADLVARIF
                     DVKQRPTFDPLIVHTSSIERISDFAQSIPDALMPLAEKFWPGPLTLLVERIAKIPDLV
                     SSGLPHVAVRIPNHPLTLQLLDQLEFPLAAPSANPFGYVSPTTAQHVNDQLGDKIPFI
                     LDGGACQVGLESTIVGLIDGVITVFRLGGLSLESIEGVVGKVQVRSHSSNPQAPGMLD
                     SHYSPRKKVLLGIIPELLKNHRDQSPVLIRFSSLLSEYPEALQLVLSPKASVSEAAIN
                     LFKYLRLADQFGTSLILAELVPEIGLGRAINDRLKRAAFH"
BASE COUNT         1802 a         1024 c         1264 g         1895 t
ORIGIN      
        1 atcggagacg tgaaggaatt cttcgtagag tttgttgccg cacgattcac aaaaccacat
       61 gaaggcatcg ttttcttgtt ttggacgcac taattctact actaaaccaa cagtattttc
      121 gggacgttgg ggtgaatgag gagtgcggcc cggaagcatg aagatgtcgc cttcgttgat
      181 gtgaatatcg acaggtttgc catcctcgat gattttaagt gtcatatttc cttccaactg
      241 aaagtaaaat tcttcgcttt cgttgtagtg atagtccttt cgtttgtttg gaccgcctac
      301 taccattacg attatatcgt cggcagtttt atatacaact tgattaccta ctggaggttt
      361 gagtaagtgg cggttttcgt cgatccactt tttaaaggaa aaaggacgct ggattggcat
      421 atgcagtatt tttgaggcaa ggtaataaaa cctgaatttg tcttatggaa ctaaatctcg
      481 caggaaaaac agcgctggta tgcggtagta ctcaaggaat tggacgtgct tgtgctcgtg
      541 aattggccga agcaggtgct catattgtac ttcttgcccg tgatgaaacg cgtttaaaag
      601 aggaaaaaat tgaactcgat cgaatcaatc acctcaacaa cgactatttg gtagccgact
      661 ttaatgattg gaaacaagtt gaatcggtag ttaaagcctt tgttgcgaaa agcaatccca
      721 ttcatatttt ggtaaacaac aatggtggac ctgctgctgg tccaataacc gatgctcgtc
      781 cagaagcttt tgaagccgca tttacccaac atcttttatg taatcaagta ttggttcagg
      841 ctgtggtgaa tgaaatgatt aaagctgatt atggacgtat catcaacatc atatcgactt
      901 cggtaaaagc tcctttgcat ggattaggag tttccaatac cattcgagca gctgtgggaa
      961 attggtctaa aactatggcc aatgaattag gaaaatatgg cattacggtg aataatgttt
     1021 tgccgggtgc tactatgact ggacgtttat ctcaaatcat tacgaataaa gcggctcgca
     1081 cacaccaaga agtagctcat gtagaagagg aaatgttagg tgagattcct ttgaaacgtt
     1141 ttgccaaacc agaagaaatt gctcatgccg taacgttttt ggcttcacct gctgctgcat
     1201 atatcaatgg tattaattta cccgttgacg gtggaaggac tgctagttta tgatgtgctc
     1261 ccttcgacaa gctcagggag cgcatatatc cgagcgctga gcttttgtcg aagcgtgctg
     1321 tttaggtata ggaaggtggg tacatttcga ttagaatgtt atatctgttt tgatttgaaa
     1381 atgtgaagat ttgcgtgccc gccattttgg aggaaaaatg gggaatacat aagtttgatt
     1441 ccgctcgggg ggcttatcgt agcgagctta gattaattga tgataaaaaa tactaaatga
     1501 aattagtaag acatttttta attggactga tcattttagt ttcccatgaa attaatgctc
     1561 agggtattga gaagaccttt cgtgggcaat gggcgagtag tttttggaca tttgagtttc
     1621 attcgaataa taactacaaa cgtacttcta gtggacatta tggacatacg attgtggaag
     1681 ggacatacag attttataat gatacattag aaatattgaa tggttatgaa gaatcgaatg
     1741 ggacaattaa tcgttattat ttgcttgatg gaaatcggat tatagatcta gaaattttat
     1801 acgattatta tgctgaagaa ggatttccta taagttggaa aaacaaacgt atcctagtaa
     1861 atcagactaa aggcatgcca ccgaagtatt ggtacgaacc cgagatattg aaatatatac
     1921 gagattaaat ctttctacgt tatacctgaa tatcctatga aaaaacacct acttataagt
     1981 ttgttgctct gcttagttta cgcaggaagt tttggtcaaa caaggcatgg agacagtgct
     2041 tggtatgaag gccggtatcg gtcgcaggaa gaaatgaatg atattcgcaa gaaagaggct
     2101 aaagagaaat tcgaatcctg gtttatgcct ggattaggtt attcgatgta tcagccaaaa
     2161 aaacgtgatt ctactggatt attttcgggc ttaacagttg aatatcttat ttatgcaaat
     2221 gtggatcaga atgatgatcc gggaccgagt catgttcggg tatattcgaa attgaatatt
     2281 ttgaatagtg atcggaagtc gataaactcg atgttcatgt acacgattgg tttggattta
     2341 tcgttggaga aaaatccgaa acgtcagttt ttaattccct attttggagt ggagatgggt
     2401 gggatttcgc aaaagcaatt tggctctgga attcagttta cgcctacgtt tggattgcat
     2461 cttttatcga agaaaaacat gtttatcaat atacacggag gatatgttta tccgattagg
     2521 aacttcgaaa tgctgcaagg ttggtacgga caagcaggat tgaattttgc actttggtga
     2581 aagaaaataa accaaccatt aattattatt ttctaattca gctcttttca ttttcctgta
     2641 ttgaaagaaa cgttggataa gtggttgaat taaatttgaa cctttatcac gaatgtcctc
     2701 ggcattgttt gtcgcaacat tggtaaccag tgtttgagtg ataattccta atattgatct
     2761 aacaggattt attgaatttc cccataagat ttttttagca agaatacctg ttatagaacc
     2821 aattgctaaa ttaccgattc cgcctttcat atttggcaat tccatcacgt catgaatagc
     2881 cgttttaatg atattgaaag gtttaagatt atccattact gattgaattt ggaatcccaa
     2941 ctctttatgt tgaatagcac gaattgcttc caattcctct attcgtagat tcaaggcaat
     3001 aagtgaatta ggtttcatta gtttaattta tatagattat taattattag attagaaata
     3061 ggcattttta taagaacatt tttaaatgct aaaaatataa ttgtaattag taaatagaag
     3121 caagcaacta taaagaaacc ataatatgaa acaccaaata aagagcctat ccaaattgct
     3181 gcaccaatat taatcatcat taaaacaatt aatactgaca caaaaattac gaatcctcca
     3241 gctaaagaag agaacacttc agctgatttt ccgattgttt tcaatcgaat taattcaata
     3301 tttgtttgag tatataattc tcccttttca aggattgatt ccattatagt tgcttgatct
     3361 tccatttgaa tgtgcattat tgtaatttat aatgaaatgt caaagaaata cttccgtttg
     3421 aaatagtaat tattgaatta ctcccttgtg aaattgggtt aaatgttcca gaagcaatta
     3481 cttcatcaga actcaataaa tcttcgtcaa cgagctggta tttccacgtt tcattcgtga
     3541 attgtatgtt gctattgggt gttaatgaag tagcaaacac atctgaaaca gaattagtgg
     3601 tatagtcata tcctgaactc gtgttttttc caaaatttaa tttcaattct ggaccagaac
     3661 catcgatatc ccattcagta cctgctggat ttgcagttgg aacgtttgaa atatcgatag
     3721 ttgttacata gcgatattga caacctgtat tttcatctgc ttcagaatcg tagttatcgg
     3781 cctgaatatc ggtacatcct tcttttttag tacacgaaat ggtgaacagt gaaactgaaa
     3841 aaagtaatga aagtacactt acgttaatta atttcatttg aggggatttt agtgattatt
     3901 attttgagct aattgaccta ccttttcgtt gacttgattt ttaaggtcca ttttagcatc
     3961 ttggaatttt gcttttcctg attcaacaag ctcttcggca ttgtctttta cttcggaata
     4021 tttatcttcc atttgattta gaaactcatt gaatttctct tttaatccat cagcaaaatc
     4081 atcccctttg gaaattattt tttttcgtgt atttgcccct ttatcgggag cgaataatat
     4141 tcctaaaaaa gcacctgcag caacgccagc aaggacgcct aataaaactt tacctgaatt
     4201 catggttatt taatttaaag gttatttaat cgaaatagga aatcgagtat ttgaataatt
     4261 aactagtatt aatcaattat tcttgaatcc ttcctaatag gaaaaattag aattatatga
     4321 ctaatgcttt ctttcctatg aatcaaaggt gatacctaca cctagaattt ctattatgtt
     4381 tttttcctaa aatattatgc gattcacact atttgattaa ataggtgttt ttatttaaat
     4441 aattaaaacg agtaaaatca agatttccat tctaataaaa acgcgtgagg gatgtgaagg
     4501 gcttggtccc ttgggacgga agcgaagcgt accccgtaac agcccgacct tgttgctgtg
     4561 tagtgaaacg gaatagcaaa aagggcacgc ccaaaatcaa tgaaaagcag cgcgttttaa
     4621 tcggtcattg atggcacgac caagtccgat ttcgggaacc aattcagcta gaattagcga
     4681 ggttccgaat tgatcggcga ggcggaggta tttgaagaga ttgatggctg cttcggacac
     4741 ggatgccttc ggacttaaca ctaattgcaa ggcttcggga tattcgctta ataagctgga
     4801 aaagcgaata agaacgggtg attggtcgcg gtgattttta agcagttcgg gaataatacc
     4861 caaaaggact tttttccgag gagaatagtg agaatcgagc attcccggag cttgtggatt
     4921 agaactatga gaacgaacct gtacttttcc aacgacacct tcaatggatt cgagcgataa
     4981 accacccaaa cgaaaaacag taataacacc atcaatcaag cctacgatag tactttcgag
     5041 tccaacttga caagctccac cgtcgaggat aaatgggatt ttatcaccca actgatcgtt
     5101 cacatgttga gcagtagttg gactcacata tccaaaagga ttggcactag gagcagccaa
     5161 aggaaattcg agctgatcga gcaattgcag tgttaatgga tgattgggaa tgcgaacagc
     5221 aacatgtggc aatccactag ataccaaatc ggggatttta gcgatacgct ccactaataa
     5281 agtaagcggt cccggccaaa acttttcagc taatggcatt aaagcatccg gaatggattg
     5341 tgcgaaatcg gaaatacgtt caattgaaga ggtatgaaca ataagtggat cgaacgtagg
     5401 tcgctgcttg acatcgaata tccgtgcgac taaatcggca ttgtatgcat ttcctgctaa
     5461 tccataaacg gtttctgtag gaataccaat tacctcattt tgcaacaatt gcgctgcttg
     5521 atgaatatcc gttccgatga ttgccatggg gcaaaggtaa ttgatgtgct gatgtgctgg
     5581 tgtgttgatg tgctgtttag tttacgggag gttggagctt agcgttatga atgtaggagc
     5641 tgtaacttaa tgatttgccc ttcgactatg ctcaggggcc agatttaaag ttaggtttaa
     5701 gttaatctga acccgcgcgc tgagcttgtc gaagggtgct ggtttgatgt gctcccattg
     5761 acaggctcaa ggagcgagaa tgttatcttt cgctgggctt gtctatgctg ataccgagcg
     5821 ctgagcttgt cgaagcggga tttgtgtttt tcggtcgtta ctttttttca acaaatccca
     5881 ttttcggctg ttgggctagc ttccttatct tcgcaacgtg cgcatatatc ctgaagacag
     5941 tatttctagg tttgaatttg atcgtattcg gtcgacggtg gagag
//
LOCUS       DAOOQW010000147         5965 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_16166_length_5965_cov_4.683418, whole genome shotgun sequence.
ACCESSION   DAOOQW010000147 DAOOQW010000000
VERSION     DAOOQW010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5965
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16166_length_5965_cov_4.683418"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="PK281_08455"
     CDS             complement(<1..342)
                     /locus_tag="PK281_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014165260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HOY49417.1"
                     /translation="MPKLSTDLHDPRTTIYIKGARVHNLKNVDVALPRNKLVVITGLS
                     GSGKSSLAFDTLYAEGQRRYVESLSSYARQFLGRLDKPKVDYIKGIAPAIAIEQKVNT
                     TNARSTVGTSTE"
     gene            529..1113
                     /locus_tag="PK281_08460"
     CDS             529..1113
                     /locus_tag="PK281_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013552166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOY49418.1"
                     /translation="MRLAQVQDQELVRNYINGHEEALSVLVSRHQQKVFTYIRMLVKD
                     SALAEDIFQDVFVKVIHTLKSGNYNEEGKFLPWIMRIAHNLSIDHFRKSKRIPVVQPK
                     DDFDIFRTLRIEDDNVEDKMVKDQILKDVKKLIKELPAEQQEVLILRHYADMSFQEIA
                     DFTNVSINTALGRMRYALINLRKIVKQKDMILSA"
     gene            1179..2843
                     /locus_tag="PK281_08465"
     CDS             1179..2843
                     /locus_tag="PK281_08465"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOY49419.1"
                     /translation="MNSLILAGRTMANSYLKSPIKRDLLFLGILLLISLFYDYQNILF
                     YRPQGIHAWRQTDCLSLTYSYYKENAPFLEPQLHIQFSDQQTSGKTIGEFPILYYFIG
                     NLWKLFGHQEYMYRLFVLLLSYLGLFYLYRIAWKLTNSWMLAVSAPIILLSSPIFAFY
                     AANFLTNIPALSTMLIGWYFIARYIENARMKHFYWAIFFFLLTGLLKVSTLMTVMLIP
                     LAILLEMIGLHKLKKEGKAFAKPVLQLGLFVFMLALVMGWYKYADYYMSIHEGRYTFT
                     KPVTLWETPRELSDMYLTHFKESLLPQIHRVVTMALIIIAGIFLFIRHRKVEKFWLIG
                     IPILLVGYVFFVLLFIYSLDGHDYYHIDFLAVLIFIYLAFIRYISREESVLIQSPMAQ
                     GIFSIFLVFNVMGCANNLYVRYHNCPESDLPYRQIFCSRKEVDLLGYPQWQSDIMRTY
                     GTVEPTLRKAGISREDRFLTMDDGSFNVTLYLMDQKGWPNMDGSLGDSLKIMDKVQLG
                     AKFMIMHRPETLEQAWLKPFMSHPFLQYENLHVFDLRPFAAVKQSS"
     assembly_gap    2939..3038
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3039..4545)
                     /locus_tag="PK281_08470"
     CDS             complement(<3039..4545)
                     /locus_tag="PK281_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49420.1"
                     /translation="MPFSEENKTAKPQFLTIQKLIKLLDDFHKEELCNYLSEIKAIIP
                     LKLVESIDSSLNETISSDDLCKKVYGNNDIQTRKSFNQLASHTFRLASYLSRNYPNYL
                     LHNVSRIERLINEGKLNEANILADILLDVAERIYDYTTQIQTLKFQAQQAQMYKSHSE
                     TVRIYTRIQEIQSYEQIINELYLYIRKNFNISVKDDSVLQSIDKHFEYLKTFHSHTEP
                     AIRILSKYSRFFLLYYYRPTEYLTERTQLEISDFDDELNKNQTVVMPFLEDIYSKFLF
                     YKLNLSTTDLSSKEGKAEYQKMLLHNKFLKFWENYVNIPELYAITIKSTYYLSRYHSI
                     NHRVDFRNVIPAEDRQDITRLAQRCEELIKKDIWEPDHVNDLIHFRLTWSALLLLGDH
                     SDIERGVDNLEKLMTTYQQITFSESMDSIFICLMLGYFALDDFKMCVESYKRYIKLSK
                     GRVVNSENDLEIHTYYYVSQWLSSQRKQYLRKIQENYQRAGQHNSRQVIQQT"
     gene            complement(4575..>5965)
                     /locus_tag="PK281_08475"
     CDS             complement(4575..>5965)
                     /locus_tag="PK281_08475"
                     /inference="COORDINATES: protein motif:HMM:NF024554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HOY49421.1"
                     /translation="NRMIFPIHNLSGKVIGFGGRILVNDKKQAKYLNSPESEVYHKSA
                     VLYGIHLARKAIMTQDEALLVEGYTDVISMHQAGIENVVSSSGTSLTADQVKIIQRYT
                     KNVTILYDGDPAGIKASFRGLDMILEQGMNVKVVMFPDGEDPDSFARSNSPVFVKEYI
                     RQEAKDFIEFKTSILQADTQGDPIRTAEMIREIIHSLSLIPDPLLRSLYVRKCAARLD
                     MEEQTLILELNNQVRNARKKLDKERDRERTPENLSEAPPDNFWADTEDESTPSHTEEL
                     TNLAGASQESDIIRILIRFANHTLLFHSTNEKGEAQDIQLRVGDFILQELLGDELVPM
                     NPLLHNIFQLFTEVGENDYPSESFFLHHENHEISSKAIHLTSNPHTLSEHWKEKHQIY
                     ITDEEMLMRKMVLSAVYSYKLRRVQGMQHEVDQQIKQSKDENEQMELLQHSMGLQQAK
                     LELAKQLSYVIL"
BASE COUNT         1620 a         1098 c         1223 g         1924 t
ORIGIN      
        1 ttcagttgaa gttccaacgg ttgaacgcgc attggttgta tttacttttt gttcaattgc
       61 aatcgctgga gctattcctt tgatgtaatc tactttaggt ttatccaaac gacccaaaaa
      121 ctgacgtgcg taagagctaa ggctttctac atagcgtctt tgcccttcgg cgtaaagggt
      181 atcgaaggct aaagatgact ttcccgaacc tgataatccg gtaataacta ccagtttatt
      241 gcggggaagt gctacgtcta cattttttaa attgtggacg cgggcacctt taatatatat
      301 ggtggtacgg ggatcgtgga gatcagtgga gagctttggc atctgtaaga tatttgcaaa
      361 attatgaatt caaacttgca tttgtcaaaa acctttattt actttgctta taacgatcga
      421 caaaccgcga agttttgccg atcttttttt aagaaccaat ttagaagcgc ctatagcata
      481 cacctctact ttttaaaata actcacacag tttaaaaagg aggtctgtat gcgtttagct
      541 caggttcagg atcaggaact ggtccgcaac tacatcaacg gtcacgaaga agcattatct
      601 gtattggtta gtcgccatca gcagaaagta tttacctaca tccgaatgtt ggtgaaagac
      661 tctgcattgg cggaagacat ttttcaggat gttttcgtga aggttattca cacccttaaa
      721 tcagggaatt acaatgagga agggaaattt ctgccttgga ttatgcgaat tgcgcacaat
      781 ctaagcattg accacttccg taaatctaaa cgcattccgg tagttcagcc gaaggatgat
      841 tttgacatct ttcgtacact tcggattgag gacgacaacg tagaagataa aatggtgaag
      901 gaccagattc tgaaagacgt gaagaagctt atcaaagagc ttcccgctga acaacaagaa
      961 gtattaatcc ttcgtcatta tgctgatatg agttttcagg aaattgctga tttcacaaac
     1021 gtgagcatca atactgcatt gggaagaatg cggtatgctt taatcaactt gcgaaaaatt
     1081 gtgaagcaaa aagatatgat tctttctgca taatcggcga aatttgaaag caaagccagt
     1141 ccccaataag gactggcttt tttgttttct atactggtat gaattctctt attttggctg
     1201 gaagaactat ggcaaattcc tatctaaaat cacccattaa aagagatcta ctttttttgg
     1261 ggatacttct gctgatttca ctgttttatg attatcagaa tattctgttt tatcgtccgc
     1321 aaggaattca tgcttggcgt cagaccgact gtttatcctt aacttatagt tattacaagg
     1381 agaacgcccc ctttttggag cctcagttac acattcaatt tagtgatcaa caaaccagtg
     1441 ggaaaacgat aggagaattc ccaattctct attatttcat aggtaatctc tggaaactat
     1501 tcgggcatca agaatacatg tatcgtttgt ttgtattgtt gttgagctat ctcggacttt
     1561 tctatttgta tcgaattgct tggaagctga caaattcatg gatgctggct gtttccgccc
     1621 caatcatcct tttaagctcg cccatatttg ctttttatgc agctaatttc ttaacgaata
     1681 ttcctgcttt aagcaccatg ttgattggtt ggtatttcat tgctcgatac attgaaaatg
     1741 cccgaatgaa acatttttac tgggccatat ttttctttct gttaacaggt ttactcaagg
     1801 tttccacttt gatgactgtg atgttaattc cactggccat tttgttggag atgatcggat
     1861 tgcacaagct taaaaaagaa ggaaaagctt ttgccaaacc agttcttcaa cttggacttt
     1921 ttgtgtttat gctagcactt gttatgggtt ggtataagta tgccgattat tacatgtcga
     1981 tacacgaggg acgttacacc tttaccaaac ctgtaacact ttgggaaact ccacgtgaat
     2041 tgagtgatat gtatctgact cattttaaag agtctttact tcctcaaatt caccgtgttg
     2101 taacgatggc tttgattata atagcaggaa tttttctttt tattcgtcac cgaaaagtgg
     2161 agaagttctg gctaatcggg atacctattt tattagttgg atatgtattt ttcgtgcttc
     2221 tgtttattta ttcactcgac ggtcacgatt attaccacat tgatttctta gccgttctga
     2281 ttttcattta cctcgcattt atccgataca tcagtcggga agaatctgtc ttgattcaat
     2341 cacctatggc gcaggggatt ttcagcattt ttctcgtgtt taatgtgatg ggatgcgcta
     2401 ataacttgta tgtgcgttac cataattgcc ctgaaagtga tttgccttat cgacagattt
     2461 tttgcagtcg gaaagaagtt gatttattgg gttatccgca gtggcagagc gatattatgc
     2521 gcacatacgg cactgttgaa ccaactttac gcaaagccgg aattagccgg gaagatcgtt
     2581 ttttaacgat ggatgatgga tcctttaacg tgactttgta cctaatggat caaaagggat
     2641 ggcccaacat ggacggttct ttgggcgata gtttgaaaat tatggacaag gttcaattag
     2701 gtgctaaatt catgattatg catcgccccg aaacacttga acaagcttgg ttaaaaccct
     2761 ttatgagtca cccgttttta cagtatgaaa atttgcacgt atttgattta cgtccattcg
     2821 cagcggtaaa gcaatcgagt taaaggctta caggaatatt gaagtaagaa actaattcca
     2881 gaatagtttg ctgaattact tggcgactgt tgtgttgtcc ggcacgctga taattctcnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnta gtttgctgaa ttacttggcg
     3061 actgttgtgt tgtccggcac gctgataatt ctcctgaatt ttacgtaagt actgtttacg
     3121 ttgactactc agccattgtg aaacgtagta atacgtatga atttcgaggt cgttttcaga
     3181 gtttacaact cgcccttttg atagtttgat gtagcgttta tacgattcaa cacacatttt
     3241 aaaatcatcc aaagcaaaat acccaagcat aaggcagatg aagatactgt ccattgattc
     3301 ggagaaggta atttgctgat aggttgtcat cagcttttcg aggttatcaa ctccacgctc
     3361 aatatccgaa tggtcaccca ataataacaa tgccgaccaa gttaagcgga agtgaatcaa
     3421 gtcgtttaca tgatccggtt cccatatgtc ttttttgatt agttcttcgc atcgctgggc
     3481 taatcgagta atatcctgac gatcttctgc aggtataaca tttctaaaat caacccggtg
     3541 attaatgcta tggtagcgag ataagtagta tgtcgatttg atcgtaatag cgtacaactc
     3601 agggatgttt acataattct cccagaattt caagaatttg ttgtgaagca gcattttttg
     3661 gtactcagct ttcccttctt tactgcttaa atcggtggtg cttagattga gtttgtagaa
     3721 taagaattta ctgtaaatgt cttctagaaa cggcatgaca accgtttggt ttttgttgag
     3781 ttcatcgtcg aaatccgaaa tctcaagttg agtacgttcg gttaggtatt cagtagggcg
     3841 atagtaatac aacaagaaga atcgagaata cttgctcagg atacgaattg ccggttcggt
     3901 atgcgaatgg aatgttttta agtactcgaa atgtttgtcg attgactgta ataccgagtc
     3961 gtctttaacg ctgatgttga agtttttacg gatgtacaga tagagttcgt tgatgatttg
     4021 ctcgtaggat tgaatctcct gaatgcgtgt ataaatgcga accgtttcgg aatgcgactt
     4081 atacatctga gcctgctgag cttgaaattt caaggtttgg atttgcgtgg tatagtcgta
     4141 aatccgttca gcaacgtcga gtagaatatc agcaaggatg ttggcttcat tcaatttgcc
     4201 ttcattgata agtcgctcaa ttcgactcac attatgaagc aagtagttcg gataattacg
     4261 acttaaatac gaagcaagtc ggaaagtatg cgaggcaagt tgattgaaac tctttcgggt
     4321 ttgaatatca ttattaccgt aaaccttttt gcaaagatca tctgagctaa tggtctcatt
     4381 aagtgagctg tcgattgatt caaccagctt aagaggtatg atagctttaa tttcgctcag
     4441 ataattacag agttcttctt tatgaaaatc atctagtaat ttaatgagtt tctgaatggt
     4501 tagaaactga ggcttggcgg ttttattttc ttcagaaaaa ggcattgtga aatatactta
     4561 agaagaagca ggaattaaag aataacatac gaaagttgct tggccaattc tagtttggct
     4621 tgttgaagtc ccatggagtg ttgtaaaagc tccatttgtt cgttttcatc tttcgattgt
     4681 ttgatttgtt gatcaacttc atgctgcatg ccctgaacgc ggcgaagttt gtatgagtac
     4741 acggcgctta gtaccatttt tcgcatcagc atttcctcat cggtaatgta aatctgatgt
     4801 ttttctttcc aatgttcact gagtgtatgt ggattactcg tgagatggat tgccttgctg
     4861 gagatttcgt ggttttcgtg atgtaaaaag aagctttcgg atggataatc attttcaccc
     4921 acttctgtaa aaagctgaaa gatattatgg agcaaaggat tcattggaac tagttcatcg
     4981 cccaataatt cctgcaaaat aaaatccccc acgcgtaatt gtatgtcttg agcttctccc
     5041 ttttcgttgg tagaatggaa aagtagcgta tgatttgcga agcgaattaa tatccgaatg
     5101 atatccgact cttgtgaagc accagccaga ttggttaatt cttctgtatg gcttggggtt
     5161 gattcatcct ccgtatcggc ccagaaatta tcgggtggtg cttctgataa attttcgggt
     5221 gtacgttctc ggtcacgttc tttatccagt tttttacgcg catttctcac ttggttattt
     5281 aattccaaaa tgagggtttg ctcttccata tccagcctcg ctgcacattt tcgtacataa
     5341 agcgagcgca ataatggatc gggaattaaa gacagagaat gaatgatttc ccgaatcatt
     5401 tcggcagtac gaataggatc gccctgcgta tctgcttgaa gaatggatgt tttaaattcg
     5461 ataaagtctt tggcttcctg gcggatgtat tccttcacaa aaacaggtga gttgcttcgt
     5521 gcaaaagaat cgggatcttc cccatcggga aacatcacca ctttcacatt catgccttgt
     5581 tcgagaatca tatccaatcc tcggaatgat gccttaatac cagccggatc gccatcataa
     5641 agaatcgtta cattctttgt atagcgctgt atgattttaa cttgatcggc tgtaagcgat
     5701 gttccagaag aggaaaccac attttcgatt ccagcctgat gcatcgaaat aacatcggta
     5761 tatccttcca ctaacaaagc ctcatcctgt gtcatgatag cttttcgcgc caggtggatt
     5821 ccgtatagga cagcactttt gtgatatact tcggactcag gtgaattgag gtatttagct
     5881 tgcttcttat cgtttactaa aatccttcca ccaaaaccaa tcacctttcc actcaggtta
     5941 tgaatgggaa aaatcattcg attcc
//
LOCUS       DAOOQW010000148         5850 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_16563_length_5850_cov_5.348059, whole genome shotgun sequence.
ACCESSION   DAOOQW010000148 DAOOQW010000000
VERSION     DAOOQW010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5850
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16563_length_5850_cov_5.348059"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="PK281_08480"
     CDS             complement(<1..222)
                     /locus_tag="PK281_08480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49422.1"
                     /translation="MSAVYLEFQFQTGKDRQLKDILISGLADIGFESFMDDETGFLGY
                     VQEPDFKKDDFLELIVSFPAKPAYSFNRIE"
     gene            complement(219..971)
                     /gene="tpiA"
                     /locus_tag="PK281_08485"
     CDS             complement(219..971)
                     /gene="tpiA"
                     /locus_tag="PK281_08485"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008636929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOY49423.1"
                     /translation="MRKKIVAGNWKMNTSLTEALGLAREIRDQLNDSENAQLIVCPPF
                     TWIVPVRATLADSGISVGAQNCSEQAKGAFTGEVSANMLVSAGADYVILGHSERRALY
                     HETDELIGKKVKSALENGLHVIFCCGETLAEREAGKLEEVLKNQVSGGLNGLNEAELK
                     KVIIAYEPVWAIGTGVTASPEQAQEVHAFLRNYLTTLFSSSIAQNMSILYGGSCNAQN
                     AASLFSQPDIDGGLIGGASLKAADFLTIARSF"
     gene            1086..2015
                     /locus_tag="PK281_08490"
     CDS             1086..2015
                     /locus_tag="PK281_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014791198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOY49424.1"
                     /translation="MNILFADTAHPFLEEALTKAGHTCFYDPELNRSKAKEILNQYGG
                     VVIRSRFKFDRELLDAGKNLRFIARVGAGMENIDQAYAESLGIVCLNAPEGNRNAVAE
                     QALGMLLMLFNNLLRADAEVRKGIWIREENRGIELCGKTLAIIGYGNTGSTFAKRLRG
                     FDVKVLAVDPYKTGFGDEFVQESTMDEVFREADVVSLHIPLTDETRYLVNENWLNRFE
                     KNIYLINTARGKCLNTTDFVKALNSGKVLGAALDVLEYETVSFEQLDQIPAPLQALID
                     SDKTVLSPHIAGWTHESNRKMAEILVGKIGELV"
     gene            complement(2146..5226)
                     /locus_tag="PK281_08495"
     CDS             complement(2146..5226)
                     /locus_tag="PK281_08495"
                     /inference="COORDINATES: protein motif:HMM:NF021021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M64 family metallopeptidase"
                     /protein_id="HOY49425.1"
                     /translation="MNKEMKKITYILTFISTFCNFYLEGQSYFSGISNEQYLSLPSNP
                     SEVDNNKIHLVILSDGYTNSTSDENKFLGVINPTGIQERGDAEEFLFDISRRAPYKEY
                     FNYFRIYKVFEPSENSGIEHPANLSANAPSCENTTITPQQNFNSRFNSSLDRYGFHAG
                     LATTDIYASIIDFLSPHFGTVTEDGIIKLPPNVLVVVIVNDANYAGTANIKRRICLIA
                     GRNTTYNPTFPYNSISSNGYIDRNKDGAGATHEFSHIFGGLQDEYWFPCPTTNDFNCV
                     DFHNAPNRWNSTSSQPTPWLHWMNYSSISTFGIFGITGTCANPQWTSSPPSPDYKAPR
                     IQGYCFMGTLYATEGLCAVCREAIIERIHNLVSPINSSSPSTNNGDVFPISSSQNFEV
                     NLTIPLRTDIGTSSMVVNWFLLDENESNVLESLHQEVPSVTPVNPAAAIYKFTNSSHP
                     SICDWFNGKEAGNYKIKVEVYDRAGASEDLSDNSRWVRDPNHSTHKHYVIWNVYYSGI
                     AGIDLYSSDSDLDLGAEPSYVAPVNWESPDIWVTNFDNLTGLEHENPVYVDGEPANVH
                     VWIKNRGCKVFDPTATPTPDAGTVNFYWSKANTVLDWTDSWSPIPFLDHPAAIQGDYI
                     GQTSITEMVDAAAEPVQVSVAWDVPDPSIYDFLGSDMVHFCLLTSLDATGDGITDPTE
                     GNTYEYVRANNNVAQKNLTVIDDQTFTGAAGDGNTEDRVTGAAVIIGNPSSTPQTFRL
                     KFKVPAEETGKPITEEAEVRIILSDTLWNRWTRGSQTGSGVQVYNASRRQILLTTDSA
                     WIGNMNLDTAEFEPLYVSVNFLTQERSSKELFTLRVLHTLEDSSSTILGGETYLIKPD
                     PYRTSFTADAGNDKELLVGTTWNQSAKTLTETATYNWYNSSGTKIHSGATLSDTTTTV
                     SSETYTLEVIAQSDGYKDYDEVTITSKLGKINSIVPNPINSGTFTIDYSVSTSASSPK
                     IKVLNINSGIYTEHTISTGNGTLSLAISGYVAGSYSVILICSSQNADDELLIIQ"
     gene            complement(5223..>5850)
                     /locus_tag="PK281_08500"
     CDS             complement(5223..>5850)
                     /locus_tag="PK281_08500"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY49426.1"
                     /translation="DDLVANFPSCSHDLIVIFPRVSGFQFGEPILLETPYFFRDIELF
                     DINNDSKVDIIFSNWNQESSVAIGYYLNENNFNFDTWQILSDSPYGFNNLYPIDYDFD
                     SDIDLIADNFYGKISLFKNLDFHVSQENIKQELRIYPNPAQFYIQIQNEIPPNSAFNI
                     ISMEQKIIKSGYINNGQSIFLYGISSGVYILEITSKNQVSKSKIIIIQ"
BASE COUNT         1728 a         1098 c         1207 g         1817 t
ORIGIN      
        1 ctcgattcgg ttgaaggaat aggctggttt tgcagggaaa ctaacaatca attccagaaa
       61 atcatccttc ttgaaatcag gttcttggac atagcctaaa aagccggttt catcatccat
      121 gaaactttca aaaccaatat cagctaatcc ggagattagg atgtctttca actggcggtc
      181 tttgcccgtt tgaaactgaa attcgaggta tacggcgctc aaaacgatcg ggctatagtt
      241 agaaaatcgg ctgctttaag ggatgctcca ccaatcaaac cgccatcaat atcgggctgt
      301 gagaaaagac tcgctgcatt ttgtgcattg caacttccac catataaaat ggacatgttt
      361 tgggcgatgg aactcgaaaa cagcgtagtg agataatttc gtaaaaacgc atgcacttct
      421 tgagcttgtt cgggactagc tgttacgcct gttccaattg cccacactgg ttcgtatgcg
      481 ataatgacct ttttgagttc tgcttcattt aagccattca atccacccga aacttgattt
      541 ttaagcactt cttcgagctt tccagcttca cgttcggcta atgtttctcc acagcaaaaa
      601 atgacgtgta atccattttc gagggcggat tttacttttt taccaatgag ttcatcggtt
      661 tcgtggtaga gcgcacgacg ttcgctgtga cctaaaatca cgtaatcggc acctgctgaa
      721 accagcatat tagctgaaac ttctcccgta aatgcacctt tcgcttgttc tgaacaattt
      781 tgcgcaccta ccgaaattcc cgaatctgcc agtgttgcac gaacaggaac aatccaagta
      841 aagggtggac aaacgatcaa ttgagcattc tccgaatcgt tgagttgatc tcgtatttca
      901 cgcgccaaac ctagcgcttc ggtgagcgag gtattcattt tccagtttcc ggcgactatc
      961 tttttacgca taggtgggtg tttgggcaaa tatagagata gtacacagag tgcaatcggg
     1021 tgaataaacg gaatctatac acgtgttttg agtagaaatg aaccatgaag ccttattttt
     1081 accccatgaa tattctcttt gccgacactg cccatccatt tctcgaagaa gctttaacga
     1141 aagcagggca tacctgtttt tatgatccag aattgaatcg tagcaaggca aaggagattt
     1201 tgaatcaata cggtggtgtg gtaattcgga gtcggtttaa gtttgatcgt gagcttttgg
     1261 atgctgggaa aaatcttcgc tttatcgcgc gggtaggggc gggcatggaa aatatagatc
     1321 aagcctatgc agaatctttg ggaatagtgt gtttgaatgc acccgaaggc aaccgaaatg
     1381 cagtggcaga gcaggcttta ggtatgttgt tgatgttgtt caataacctg ttacgcgctg
     1441 atgccgaagt gcgaaaaggc atttggattc gggaagaaaa tcggggtatt gaattatgcg
     1501 ggaaaacgct tgctattatc ggctatggca ataccggaag cacctttgcc aagcgattgc
     1561 gtggttttga tgtgaaggtt ttagcggttg atccatacaa aaccggattt ggtgatgaat
     1621 ttgtccaaga atctactatg gacgaagttt ttcgcgaggc ggatgtggtt agcctgcaca
     1681 ttcccttaac cgatgaaaca cgttatttag taaatgaaaa ctggctgaat cgtttcgaaa
     1741 agaatatcta tctcatcaac accgcccgtg gaaaatgcct caacaccacc gatttcgtca
     1801 aagcgttaaa ttcgggtaaa gtcttaggtg ccgcgctcga tgtgctcgaa tatgaaaccg
     1861 tttcgtttga acaattagat cagattcctg ctcctttgca agcattaatt gatagtgata
     1921 agacagtgct tagtccgcac attgccggct ggacgcatga atcgaatcgg aaaatggcgg
     1981 agatactggt tgggaagatt ggggagttgg tttagattga gcctcggact gaagttcaag
     2041 ggtgctgatt tttttgagaa aattttaatc ctaatctttc attttcttaa ttattggaaa
     2101 ttgaaagtta aaatgaagtt ttaagattct tcgggttagg tttctttatt gaattatcaa
     2161 tagttcatca tcagcgtttt gactgctaca aattagaatt acactatacg aacctgctac
     2221 atatccagaa atagccaaag acaaagtgcc atttccggta ctgatagtat gttcagtata
     2281 tatcccagaa tttatattca acacttttat tttcggacta ctagcagaag tagatacgga
     2341 ataatcgata gtgaatgtgc cactattaat tgggttaggt acgatactgt ttattttacc
     2401 caatttagat gtaatggtca cttcatcgta atctttgtaa ccatccgatt gagcaataac
     2461 ttccagcgta taggtttctg aagaaacggt cgtagttgta tcggaaagtg ttgctcccga
     2521 atgaattttg gtgccacttg aattgtacca gttatatgtg gctgtttctg ttaaagtttt
     2581 tgcactttga ttccaggttg ttcccactaa caattcttta tcattaccag catcagcagt
     2641 aaatgaagta cgataaggat ctggctttat gagatatgtt tctccaccta aaattgttga
     2701 acttgaatcc tctaacgtgt gaagtactcg taaagtaaag agttcttttg aggagcgctc
     2761 ttgagttaag aaattgacac ttacatacag cggttcaaat tctgcggtat ctagattcat
     2821 attaccaatc catgctgaat ctgttgtcaa cagtatttgc cggcgtgatg cattataaac
     2881 ctgaacgccg gagcctgttt ggcttccacg tgtccagcgg ttccacaatg tatcactgag
     2941 gatgatccgg acttccgctt cttcggtgat gggtttaccg gtttcttctg ccggaacttt
     3001 aaactttaat cgaaatgtct gtggggtgga agagggattg ccaataatca ctgccgcacc
     3061 ggtgacacga tcttcggtat ttccatctcc tgctgctccg gtaaatgttt gatcgtctat
     3121 tacggtcagg ttcttttgag cgacgttgtt attggcccta acatattcgt aggtattgcc
     3181 ttcggtcggg tcggtgatgc catcgccggt tgcgtccaag gaggtgagca aacaaaaatg
     3241 caccatgtcg gagcccaaaa aatcataaat ggaaggatcc ggaacatccc acgcgactga
     3301 aacctgcaca ggttctgctg ctgcatccac catttccgtg atggaagtct gtccgatgta
     3361 atcgccctga atggcggcag ggtgatctaa aaacggaatg ggtgaccagc tatctgtcca
     3421 gtcgagcact gtattggctt tggaccagta aaagtttacc gtacctgcat cgggagttgg
     3481 tgttgcggtt gggtcgaaaa ctttgcaacc acggttttta atccaaacgt gcacatttgc
     3541 gggttcgccg tccacgtaaa cagggttttc atgctccaat cctgtgagat tatcaaagtt
     3601 ggtaacccag atatccggac tttcccagtt aacgggtgca acataacttg gttcggcacc
     3661 aaggtctaaa tccgaatcgg aagaatacaa atcaattcca gcaattccag aataatatac
     3721 attccaaata acatagtgtt tatgtgtact atgattggga tctctcaccc aacgcgagtt
     3781 atctgataag tcttcagatg caccagctcg atcataaact tcaactttaa ttttataatt
     3841 cccggcttct ttcccattaa accaatcaca aatactagga tgtgatgaat tagtgaattt
     3901 ataaatggca gcagcaggat taaccggtgt taccgaaggt acttcttggt gcagtgattc
     3961 tagaacattt gactcgtttt catctaatag aaaccaattt accaccatgg aactagttcc
     4021 aatatcagta cgaagaggta tagttaaatt gacttcaaag ttttgtgaac ttgaaatggg
     4081 aaatacatca ccattatttg ttgaagggga agatgagtta attgggctga ccaagttgtg
     4141 aattcgttca atgatggctt cgcgacatac ggcgcacaat ccttcagttg catacaatgt
     4201 ccccataaaa cagtatcctt gtattctggg tgctttataa tctggagatg gtggtgatga
     4261 agtccattgt ggattagcgc aagtgccagt aattccaaaa atcccaaatg ttgaaattga
     4321 agaataattc atccaatgta accaaggggt cggttgacta gaagttgaat tccatctatt
     4381 aggcgcatta tgaaaatcta cgcaattaaa gtcatttgtt gtaggacaag gaaaccaata
     4441 ttcatcttgt aatccgccaa aaatatgaga aaattcatga gttgctccag ctccatcttt
     4501 atttcggtct atataaccat tagatgagat tgaattgtaa ggaaaagttg gattatatgt
     4561 agtatttcta cccgcaatta aacagattcg tcttttgata tttgctgttc cagcataatt
     4621 tgcatcgttt acaataacaa ctactaaaac atttggaggt aatttaatta tgccatcttc
     4681 agtcacagtg ccaaaatgtg ggctcaaaaa atcaattatt gaagcatata tatcggttgt
     4741 agctaaccca gcatgaaatc catatcgatc taggcttgaa ttgaatcgtg agttaaaatt
     4801 ttgttgagga gttatagttg tattttcaca tgatggagca ttagcactta gatttgcagg
     4861 atgttcaatg ccagaatttt ctgaaggttc aaacacttta taaattctaa agtaattgaa
     4921 gtattcttta taaggtgctc tccgagaaat atcaaataga aactcttcag cgtcacctcg
     4981 ttcttgtatt ccagttggat taattacacc caaaaactta ttttcatcag atgtcgaatt
     5041 tgtatagcca tcggataaaa tgactagatg aattttatta ttatctacct cacttggatt
     5101 tgaaggtaat gataaatatt gttcatttga aattccagag aagtaggatt gtccttctaa
     5161 atagaaatta caaaaagtag aaataaatgt taggatgtat gttattttct tcatttcctt
     5221 attcattgaa tgattattat tttcgattta cttacctgat tttttgatgt tatttcaagg
     5281 atataaacac cagaagaaat gccgtataga aatattgact gaccattatt aatgtaacct
     5341 gatttaatga ttttttgctc cattgaaatt atattaaaag cactattagg tgggatttca
     5401 ttttgtattt gaatataaaa ttgagctggg ttaggatata ttctaagctc ttgttttata
     5461 ttctcttgac taacatgaaa atcaaggttt ttaaataatg aaatttttcc ataaaagtta
     5521 tctgcaatca aatctatgtc cgaatcaaaa tcataatcaa ttggatataa attattaaac
     5581 ccataaggag aatctgataa aatctgccat gtgtcaaaat taaaattgtt ctcgttaaga
     5641 tagtacccaa ttgcaacaga ggattcttga ttccaatttg aaaatatgat atcaactttg
     5701 ctatcattat taatatcaaa tagctcaatg tctctaaaga aataaggagt ttccaataga
     5761 ataggttctc caaattgaaa acctgatact ctagggaaaa tgacaattaa atcatgacta
     5821 cagcttggga aatttgctac caaatcatct
//
LOCUS       DAOOQW010000149         5832 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_16627_length_5832_cov_4.670936, whole genome shotgun sequence.
ACCESSION   DAOOQW010000149 DAOOQW010000000
VERSION     DAOOQW010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5832
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16627_length_5832_cov_4.670936"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..822
                     /locus_tag="PK281_08505"
     CDS             <1..822
                     /locus_tag="PK281_08505"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOY49427.1"
                     /translation="QEINPASAQANGIYSAVQNNGECDSPPAFTNAVVNPLPNPVISG
                     DTILCEGEETTLNLNGGPFDTQVWSTNEISSTINVPSGTWSISVGLLGCNRTVSQTVH
                     LSEPIANFNIAPDALVFLGTEQQFTDSSLASPYAPNVVYLWDFADGEFGNGTSVAHQY
                     ADTGTYSVMLVVTNTEGCVDTVFQIANVIIDVVIPNVFSPNGDGINDLFVVKYLSLYP
                     NSKLNIFTRWGSLVYSSDDYKNDWTGDDLVDGTYFYILETGKGLPPFKGTVLIER"
     gene            944..3751
                     /locus_tag="PK281_08510"
     CDS             944..3751
                     /locus_tag="PK281_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOY49428.1"
                     /translation="MRNVLFVLLTFSLTCFLTGTLDAQQLRGTITNSKNGETLIGATA
                     VVKGTTKGASADIDGNFVIDKPGTPPFTLVVSFVGFTSKEIQVKNLDQALKITLSPNE
                     ELLKEVEIVDSRLTEKQRESPLTVEAMDLLAIKETPAANFYDGLGSLKGVDLTAASLG
                     FKIINTRGFNSTSPVRSLQIIDGVDNQSPGLNFSLGNFLGSSELDVQKVDIIVGASSA
                     FYGPNAFNGVISMTTKNPFDYTGLSVQTKVGERDLFETAIRYAEKFQNKKGEDKFAYK
                     INMSFMRAYDWVADNMDATEQSVTQSGNPGGYDAVNRYGDEELGGYRNNYTSTPQLKS
                     VYPGLGAIYRTGYQEKDLVDYNTRNIKLNAALHYRLKPDVELILSSNFGTGTTIYQGE
                     NRFSLKNILFFQQRLEFTKKDKFFVRVYATNEDAGDSYDAVVSAFEMQNLSKSNNQWK
                     QDYQNSWTAGGFNAPRFIVKGFDGYPSPFNVTTYDSVMAANYDKLVTLHDSVRAKVDR
                     GNGTTTSSFFAPGTARFDSAFAAVKSRTLSNKGSLLKDKSALYHVHAEYKFQFGKFTK
                     LVVGANGRMYRPNSDGTIFSDTLVIDQIVGSDTTYKRRVIVNKEFGAYTGIQRKFFAE
                     RLSVNATVRVDKNQNFNWISTQSFSGIYTINNNHTFRAVYSSAVRNPTLADQYLYYNV
                     GRAILLGNIDGKDSLIEITSFFNYLNTLNPNDLRYFNVDPIRPERVKTFEFGYRSTLF
                     KRLFVDASYYLSEYHDFIGYKIGLDVTFTGSVPQIQAYRLAANTNDVVYTQGFSLGMN
                     YYLFDKLELNGNYSYNILNRPNVTDEIITAFNTPMNKYNIGFSGRKIKLPFIKGERFS
                     FNANYKWVQGFRFEGSPQFTGSIPSYGMVDSQISYAVEKWKTVFKLGASNLLDNKVSM
                     VYGGPKVGRLAYFSILVDLTN"
     gene            3837..5552
                     /locus_tag="PK281_08515"
     CDS             3837..5552
                     /locus_tag="PK281_08515"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY49429.1"
                     /translation="MNKLYKLSLAVVLGGISLQANAQRYLTEVFTDAQITIQNDVQYG
                     ENLSIQNTILGALATPPQQIPPSILPLMMDVYYPTIADDNLAERPLVVYAPTGDFLPA
                     GINGSPNGSRKDSSAVNMAKQFAKRGYVCAVVDYRLGWNPFSTDQIVRTGTILNAAYK
                     GQQDTKAAVRFFRNDRATTNTYKIDPTRVVLFGEGTGGYVVMAHAFLDQPWKIGRLPG
                     LGDDKFLRTQAPDSSVIDTMRIGNFDGTDAIDVNLIPFATSGDFLKITGNVANNPSYS
                     SEANMVINMGGALGDTSWLDAGQIPMIGIHAVRDPNAPHQIGDVIVPTTGDLVIPFAS
                     GAGFNLEKANSYNNNSSFATRIYPDPITAAVESHYGQSIPFAGSTINVGTGKGLLPFI
                     LPENTTYTLNHGSPWQFWNSNQATANYPVTVAPGVTITTHQASAASNPAMATSNEVGR
                     STGLTYIDTIQQYINPRIVCALNLAECGLFEVESINDLNTASALVSAYPNPSNGNVTI
                     RSIVGAGQLNSITLFDLTGRQVLAQSGLHTSSYQINRNDLPSGIYLVQVVTEKGRGTI
                     KLILE"
     gene            5616..>5832
                     /locus_tag="PK281_08520"
     CDS             5616..>5832
                     /locus_tag="PK281_08520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49430.1"
                     /translation="MPKKQQHYLFLVIILLLAFSRFLFPESDPAWWKSTDDIHDEAWW
                     AENVRRMFEGEKWPGDVWARTWAVGPLT"
BASE COUNT         1754 a         1090 c         1164 g         1824 t
ORIGIN      
        1 caggaaatta atcctgcatc tgctcaagca aatggtattt acagtgctgt tcaaaataat
       61 ggtgaatgtg attcgccacc agcctttaca aatgctgtcg taaatccttt acctaaccca
      121 gtaatatctg gtgacacgat tttatgtgaa ggagaagaaa caacgttaaa cttgaatggc
      181 ggacctttcg atactcaagt ttggtcgacc aatgaaataa gctcaacgat taatgttcca
      241 tctggtactt ggtcaataag tgtaggattg ttaggctgca atcgaaccgt tagtcaaaca
      301 gtacatttaa gtgagcctat tgcaaacttt aacattgctc ccgatgcact ggtttttctt
      361 gggaccgaac agcaatttac tgattcctct ttggcttcac cttatgctcc caatgtggtt
      421 tatttatggg attttgctga tggagaattt gggaatggaa cttcagttgc tcatcaatat
      481 gccgatacag gtacttattc ggtcatgttg gtggttacta acaccgaagg ttgtgtggat
      541 accgttttcc aaattgccaa cgtgattatt gacgttgtca ttccgaatgt tttttctccc
      601 aatggcgatg gcataaatga tttgtttgtt gtgaaatacc tttcgcttta cccgaacagt
      661 aagttgaata ttttcacacg ctggggcagc ctagtataca gtagcgatga ctacaaaaat
      721 gattggacag gagatgattt ggtcgatgga acttattttt acattctaga aacaggtaaa
      781 ggattgcctc catttaaggg aactgtttta attgaacgtt agcacatttt tcagagaaat
      841 agagtgttga tggacttgtg gagatttttt aaatgaattg cttgataaat caactgctag
      901 gtgtatttta ggaattcctt aaaatcgctt attcgtctag cctatgcgaa atgttctatt
      961 cgttctgctt acatttagtt taacctgttt tttaacagga actcttgatg cacaacagct
     1021 tcgaggcaca attacaaatt ccaaaaacgg tgaaacgctt ataggagcaa cagcagttgt
     1081 gaaaggaaca accaaaggag ctagtgctga tattgatgga aacttcgtta ttgataaacc
     1141 gggaactcca ccttttacac ttgtagtgtc gtttgttgga tttaccagca aagagattca
     1201 ggttaaaaat ttagatcagg cattaaaaat aaccttaagc ccgaatgaag aattattaaa
     1261 agaagtggaa atcgttgatt ctcgcttaac tgaaaagcaa cgcgaatcgc cacttacagt
     1321 tgaagcaatg gatctcttag ctataaaaga aactccagca gctaactttt acgatggcct
     1381 tggctctttg aaaggtgttg atttaacagc tgcaagcttg gggttcaaaa tcatcaatac
     1441 acgtggtttc aatagcactt ctccggttcg atcattacaa ataattgatg gggttgataa
     1501 tcagtcccca ggtttgaatt tctccttagg taatttcttg ggatcatccg aattggatgt
     1561 tcagaaagtt gatataatcg tgggtgcaag ctctgctttt tatggtccaa atgcctttaa
     1621 tggagtgatt agcatgacca ctaaaaatcc gttcgattac acgggtttat cggtgcaaac
     1681 taaagttgga gaacgcgatt tatttgaaac agcgattcgc tatgcggaga agtttcagaa
     1741 caaaaagggc gaagataaat ttgcctataa aattaatatg tcgttcatgc gtgcctacga
     1801 ttgggtagcc gataacatgg atgcaacgga gcaatctgtc actcaatcgg gaaatcctgg
     1861 aggatatgat gctgttaatc gatatggcga tgaagaatta ggtggttatc gaaataatta
     1921 caccagtact cctcaattaa agtcggttta tcccggtctt ggtgccattt atcgtactgg
     1981 ctatcaagaa aaagacttag ttgattacaa cacacgaaat atcaagctaa atgcagcttt
     2041 acattatcgt ctcaaaccgg atgtagaact tatcctcagc agtaatttcg gaacaggtac
     2101 taccatttac caaggtgaga atcgctttag tttgaaaaac atcttgttct ttcaacaacg
     2161 tttggaattt acgaagaagg ataagttttt tgtccgtgta tacgcaacta acgaagacgc
     2221 aggtgattcc tacgatgctg ttgtatcggc tttcgaaatg cagaatttgt ctaagtcgaa
     2281 caatcagtgg aaacaggatt atcagaatag ctggacagca ggtggtttca atgcgcctcg
     2341 tttcattgta aaagggtttg atggatatcc atctccattc aatgtaacaa cgtatgattc
     2401 agtcatggca gctaattatg ataaattagt tacgttacat gattctgttc gggctaaggt
     2461 tgatcgtggt aatggaacaa ctacaagttc cttttttgca ccaggaaccg ctcgttttga
     2521 ttctgctttt gcagccgtaa aatcccgcac tttatcgaat aaaggttcct tgctaaaaga
     2581 taaatctgcc ctttatcatg ttcatgctga atacaaattc caatttggaa aattcaccaa
     2641 acttgtagtg ggagcaaacg gacgaatgta tcgtcccaat tcagatggaa ctattttcag
     2701 tgatacctta gtaattgatc aaattgtagg ttccgataca acctacaaac gcagagtaat
     2761 tgttaataag gaatttggcg catatacagg tattcaacgt aagttctttg ccgagcgttt
     2821 gagtgtaaat gccacagttc gtgtcgataa aaaccaaaat ttcaattgga tttccaccca
     2881 gagtttttcg ggtatttaca cgatcaataa taaccatacg tttagagcag tttattcgtc
     2941 ggccgttcgt aatccaactt tagcagatca atatttgtat tacaatgttg gtcgtgcgat
     3001 attacttgga aatattgatg gtaaagattc gctaatagaa attacctctt ttttcaacta
     3061 cctcaacacg ttaaatccta atgatttacg ttatttcaat gttgatccaa ttcgaccaga
     3121 gcgggtaaaa acattcgagt ttggttatag aagtaccttg ttcaaacgct tgtttgtaga
     3181 tgcaagttat tacctatccg agtatcacga ttttattgga tacaaaatcg ggttagatgt
     3241 aacgtttaca ggaagtgtac ctcaaattca agcatatcgt ttagctgcca atacaaatga
     3301 tgtggtttat acgcaaggtt tttcacttgg tatgaattat tacctgtttg ataaactgga
     3361 acttaatggg aattactcct acaatatttt gaatcgcccg aatgtgacag atgaaatcat
     3421 cactgccttc aatactccta tgaacaaata caatattgga tttagtggtc ggaaaataaa
     3481 actacctttc attaaaggcg aacgtttttc atttaacgca aattacaaat gggttcaagg
     3541 attccgtttt gaaggctctc ctcagtttac aggatctatt ccatcttatg gaatggtaga
     3601 tagtcaaatt agctacgccg ttgaaaaatg gaaaacagtt ttcaaactcg gagcttcgaa
     3661 tttattagac aataaagtgt cgatggtata tggtggacca aaggttggtc gattagccta
     3721 tttctctata cttgttgatc ttacaaacta attagaagct tattaccgga atggcttgta
     3781 caagaaaaaa tcttcatcaa ctttgtaact ggttttaaaa ataaaatccc acaatcatga
     3841 ataaactata caaactttca ctagctgttg tccttggagg aatttccctt caagccaacg
     3901 cccagcgata cctgacagag gtatttacgg atgcacagat tacgattcaa aatgatgtac
     3961 agtatggtga aaacctatct atacaaaata ctattctagg agcacttgca actcctcctc
     4021 aacaaattcc accaagcatt ttgccattga tgatggatgt ttattatcca acaattgccg
     4081 atgataatct agctgagcgc ccgttggtag tgtatgctcc aacaggggac tttcttccag
     4141 ctggtatcaa cggttcacca aatggaagtc gtaaagacag ttcggctgtt aatatggcaa
     4201 aacaatttgc caaacgcgga tatgtttgtg cagtagttga ttatcgttta ggatggaatc
     4261 cattctcaac tgatcaaatc gttcgtacgg ggacaatctt aaatgcagct tacaaaggtc
     4321 aacaagacac taaagcagct gttcgtttct tccgtaacga ccgtgctaca accaacacat
     4381 acaaaattga ccctactcgt gtagttctat ttggtgaagg tactggtggt tatgttgtta
     4441 tggcacatgc attcttagat caaccatgga aaattggtcg cttacctggt ttaggtgatg
     4501 acaaattcct tcgtacacaa gcgccagatt catctgttat tgatacgatg cgtattggta
     4561 atttcgatgg tacagatgca attgatgtga atttaattcc attcgctaca tctggagatt
     4621 tccttaaaat tactggtaac gtagcaaaca acccaagtta ttcatctgaa gcaaacatgg
     4681 ttatcaacat gggtggcgct cttggagata cttcatggtt agatgctggg caaattccaa
     4741 tgattggaat ccatgctgtt cgtgatccga atgcaccaca tcaaattggt gacgtaattg
     4801 ttccaacaac aggagattta gttattccat ttgcttccgg agctggtttc aatttggaaa
     4861 aagctaattc ctataataac aattcatctt ttgcaactcg tatttatccg gatccaataa
     4921 ctgctgctgt tgaatctcat tatggtcaaa gcattccatt tgcaggttct accatcaacg
     4981 taggtactgg aaaaggttta cttccattta ttcttcctga aaacactact tatacattaa
     5041 atcatggaag tccttggcaa ttctggaatt ctaaccaagc aacagctaac tatccagtaa
     5101 cagttgctcc aggagttaca attacaactc accaagcttc agctgctagt aatcctgcaa
     5161 tggcaactag taacgaagta ggtcgctcaa ctggcttaac atacatcgac accattcaac
     5221 aatacatcaa tccacgtatt gtgtgtgcgt taaatttagc tgaatgtggt ttatttgaag
     5281 tagaaagcat taatgatttg aatactgctt ctgctttggt ttcagcctac cctaacccat
     5341 ctaatggaaa cgtaaccatt cgttctatcg ttggagctgg acaattgaac agtatcacac
     5401 tatttgacct aactggtcgt caagttttag ctcaaagcgg tttgcatact tctagctatc
     5461 aaattaatcg caacgatctt ccttcaggta tttacttagt acaagtagtt actgaaaaag
     5521 ggcgtggtac aattaaattg attttagaat aattcgattt tcacttttac tgtaaagtcc
     5581 tggtgttcgc atcaggactt tttttatttt ccctcatgcc taaaaagcaa cagcattatt
     5641 tatttctagt cattattctg cttctagcat tcagtcgatt tctgtttccg gaatctgatc
     5701 ctgcttggtg gaaaagcacc gatgatattc atgatgaggc ttggtgggcc gaaaatgtta
     5761 gaagaatgtt tgaaggagaa aaatggccag gtgatgtatg ggcgcgaact tgggcagttg
     5821 ggccattaac gg
//
LOCUS       DAOOQW010000150         5772 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_16844_length_5772_cov_4.529474, whole genome shotgun sequence.
ACCESSION   DAOOQW010000150 DAOOQW010000000
VERSION     DAOOQW010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5772
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16844_length_5772_cov_4.529474"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(69..2381)
                     /locus_tag="PK281_08525"
     CDS             complement(69..2381)
                     /locus_tag="PK281_08525"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HOY49431.1"
                     /translation="MKNGYTFLKGLILALAISPVSAQTSAIQVDTTLNWQQWIPTILG
                     GNCVQVSNVSFTNQPGSASRFTSGSGIGLTQGIVLSSGALGGDVSYDPSFFLGSSFPF
                     GTSDSLLEQYALQDLGYTGNSTSFDATRLEFDFTPPANQTVTIRFVFASEEYPEFAPP
                     NTNFYNDIFGFFVRQIDSTNYTNIALVPGTTLPVTIGNINAVTNPSFYIESDTSAEFA
                     FDGYTTPITATFNCIGGVTYHMIIAISDIGDSFFDSAVFLEQQSNSSQDVNGTAAAGF
                     GQMEQGYAELFGYSMAPGAFPRLDSVACGAGGNFSFTSVEDGLYLIHIVPDTSAYPLS
                     VPTYYGGAFLWEEAQTVAIACDAYATSAANLVINTGPGGISGTIGGSSEGFRLRSEEL
                     IPVPNVNVFLQDSASSELRGFARTDANGIYHFNNLAYGTYYVLPDVAGVPLIDYRKVV
                     LNATNEQEENISFQLSMDGVLNVSDVVFEPIIETGLNVEWTVTGSFRYYLGANEKIRL
                     GVDTLINDTLYQTLYIDGIENASDVFNESEKVLLGGIRQEGANLYLRYLLEQPWNSSI
                     YNKDLLYFRSDLQIGDTLHNRFSDDDISFIEITNIDTITVNGIDRTRWTYTENFYGQT
                     EYFVHGIGNSTGWFNLGYHICSDCPFFLTICWSDVNGVSAFYQDVFGEDDSWFNFNEC
                     FMISGGINEIPTLTLKLSPNPGNGLVSIQMTKQEEGFLTIRDVSGRLQMTQSVKGNQS
                     IDLGNLSAGVYIVEFRNKQGQSGFTRWIKE"
     gene            complement(2427..3017)
                     /locus_tag="PK281_08530"
     CDS             complement(2427..3017)
                     /locus_tag="PK281_08530"
                     /inference="COORDINATES: protein motif:HMM:NF020295.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HOY49432.1"
                     /translation="MPFVPTTVYAESTPNPNTMKFVANRLIVEPNRLAEYLNAEDCEG
                     APLAQELFKGGYVKSLFFSNNFVSITKTESQDWTRLTNAVREFLTDYFSKGLPVIEKY
                     PEQASPVQETPALQPVEGSTEEAIIGILEEYIRPAVEQDGGAITFRSFNDGIVTVALQ
                     GSCSGCPSSKVTLKSGIENLLVRMVPGVKEVVAEEI"
     gene            3148..4173
                     /locus_tag="PK281_08535"
     CDS             3148..4173
                     /locus_tag="PK281_08535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49433.1"
                     /translation="MKWISRFLLISLLVLQACSSDRDVHRAFYYWKTEFRFDDEDSVL
                     LADLKNDRLYLRLFDVSWDATQKKAIPIGKLSFKDDVPSFLEVVPVVYITSETLSEIP
                     AKQLPELSKNIFQLITQTLSLRRIAWKEIQLDCDWTDTNKEVYFTLLRELRKQVGVDK
                     QLSCTIRLHQIKYPEKTGIPPVDRGMLMYYNMGNLNEPGMRNSIYDPDIAARYVTYVE
                     DYPLPLDVALPVFTWGVHKKQDGNSTLVNNLTLRDVRISGLFREQASGLFLPMQGCFF
                     RGDYFQEGDELRVEEINPELCKRAAEQVSPFLKSSTFHVVLFHLDSVNTARYGQKSFE
                     TVYSVFD"
     gene            4130..>5772
                     /locus_tag="PK281_08540"
     CDS             4130..>5772
                     /locus_tag="PK281_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49434.1"
                     /translation="MVRNLSKLSILFSISFLLIPCIPQPMMGCGGGDPIDIIEMSLFS
                     PEIISSSQFEPFFLTYENYYGYNDGERSQSNESTNTSEWENYFDQLIPKESVQNLVHK
                     STIEHLDSLILMTKGKMNPGISVYTKLINSKKYNSGLSYLRKAKEVEHAFTYDYYSWN
                     PAPLDTIRQKTILPELVKGWKEEKDIRIKNRYAFQVIRVNYLLGNYADGVKFYEEQVK
                     MEPADGSMFYRTLGYKAACLYKLHRYAESNFIYARIYDEYEPHRIDAFSSFHPLEEAD
                     WNALMAMAKTTREKCLLWHLFGVYADPLKGMREIIALDPSSNLLDLLLVRSVNLAEMR
                     LLKNVPAMSEYDYLYSDYSGEETISNDAFNSWKSIDSTRATTELLDFVENTARNLPVR
                     KDVWGTSAAYLNWLANKPAACDEWISKTEKGAEGIIARQLFIIKALRMVESIDQMRVE
                     DENKVMQNLQVISTYGLDGFPLRSEATKRYIRNVMSRIYTRMNDPLMAELSETNYYQF
                     LNDSAQVQLMIDFMKRPDKRPIQNYLLGFYPVKIQELYEV"
BASE COUNT         1830 a         1027 c         1146 g         1769 t
ORIGIN      
        1 agataataga aattttcttc caatgaagtg gaatgtaaaa tattcattta tcgaatgtgg
       61 aattgccttt attccttgat ccaacgagtg aatccacttt gtccttgttt gtttctgaac
      121 tccactatat aaacaccagc actcaaatta cctaaatcaa tactttgatt tccttttacc
      181 gattgggtca tttgaagcct tccgctcaca tctcttatgg ttaaaaatcc ttcttcttgt
      241 ttagtcatct gaatgcttac taaaccatta cccggattag gtgagagctt taacgtgaga
      301 gttggaattt catttattcc accagaaatc ataaaacact cattaaaatt gaaccagcta
      361 tcatcttctc caaacacatc ttgataaaaa gctgaaacac catttacatc agaccaacaa
      421 atagttaaaa agaacgggca atcagaacaa atatgatatc ccaaattaaa ccaccctgtt
      481 gaattaccta ttccatgaac aaaatattct gtttgaccat aaaagttctc tgtgtatgtc
      541 caacgtgttc ggtcaattcc attcaccgtt attgtatcga tattggttat ctcaataaat
      601 gagatatcgt catctgaaaa gcgattgtga agtgtatcac caatctgcaa gtcagatctg
      661 aaatacaaaa gatctttatt gtaaatagat gaattccaag gttgctctaa taaatatcga
      721 agataaagat tagctccttc ttgacgaata ccgccaagaa gtactttttc actttcatta
      781 aaaacatctg atgcattttc aataccatcg atatataatg tttggtatag tgtatcatta
      841 atgagtgtat ccactcctaa acgaattttt tcatttgcac ctaagtaata ccgaaaagaa
      901 cctgtaacag tccattcaac atttaaaccc gtttcgatga ttggttcaaa aacaacatca
      961 cttacgttca atacaccatc catagaaagt tggaaactta tattctcttc ttgttcgttg
     1021 gtagcgttta aaaccacttt acgataatca attaatggaa caccggctac atcaggcaat
     1081 acataataag ttccgtaagc taaattgttg aagtggtaga tgccatttgc atctgtgcgt
     1141 gcaaaacctc gcagttctga gctggcagaa tcttggagaa atacatttac attgggaaca
     1201 ggaatcaatt cttcagaacg taagcgaaat ccttctgaac ttccaccaat ggttccactt
     1261 ataccgcccg gaccagtatt aataaccaag ttagcagcac tagttgcgta tgcatcacaa
     1321 gcgatagcaa ccgtttgggc ttcttcccat aaaaatgctc ctccgtaata tgttggaaca
     1381 gataatggat aagcggaagt atcaggtact atgtgtataa ggtataatcc atcttcaaca
     1441 ctagtaaagc tgaaatttcc tccggcgcca caagcaacac tatccaaacg tggaaatgca
     1501 cctggcgcca tggaatagcc aaataactca gcatatcctt gttccatttg tccgaaacca
     1561 gctgcagcag ttccatttac atcttgacta ctgttgcttt gttgttctaa gaaaacggcg
     1621 gaatcaaaaa agctatcacc aatgtcgcta atggcaataa tcatatggta cgtaactcca
     1681 ccaatacaat taaatgtggc tgtaattgga gttgtatatc catcgaatgc aaattcggct
     1741 gaagtatctg attcgatgta gaatgatgga tttgttacag cattgatatt tccaattgta
     1801 acaggcaaag tagttcccgg tactaatgcg atattcgtat agttggtact atcaatttgg
     1861 cgaacgaaga atccgaagat atcgttgtag aaattcgtgt ttggtggagc aaactcagga
     1921 tactcttcgg atgcgaatac gaaacgaata gtaactgttt ggttagctgg aggagtgaag
     1981 tcgaactcta gacgagtagc atcgaaagag gttgaattcc ccgtgtaacc taaatcttgt
     2041 aaagcatatt gttctagaag tgaatcactt gtgccgaaag gaaacgagct gcctaagaaa
     2101 aatgatggat cgtaactcac atcacctcca agtgcacccg aacttaaaac gattccttgt
     2161 gtaagtccaa ttccacttcc tgaagtaaat cgagatgctg atcctggttg attagtaaaa
     2221 gatacattgc tcacttgaac acaatttcct cctaaaatag taggaatcca ttgttgccaa
     2281 ttgagtgtgg tgtcaacttg aattgcggag gtttgagcag aaacagggga aatggcgagt
     2341 gccaaaatca aacccttaag aaaagtgtag ccgtttttca tcaggtgaaa ctaaacatta
     2401 tcacccataa aaaaaacaaa taggaattat atttcttctg caactacctc ttttacacca
     2461 ggaaccatgc gaaccagtaa gttttcgatt cctgatttta gtgtaacttt tgaagaagga
     2521 caacctgaac acgaaccttg aagagctact gtaacaatac cgtcattaaa tgagcgaaat
     2581 gtaattgctc caccatcttg ttccacagca ggacgaatat attcttctaa aatcccaatg
     2641 atagcttctt cggtacttcc ttcgacaggt tgtaaagcgg gagtttcttg aactggacta
     2701 gcttgttctg gatatttttc aataacgggt agacctttag agaaataatc agttagaaat
     2761 tctcgaacgg cattagttaa acgcgtccaa tcctgcgatt ctgttttggt tattgaaacg
     2821 aaattgttgc taaagaaaag cgatttcacg tatccacctt tgaacaactc ctgcgctaat
     2881 ggagcacctt cacaatcttc ggcattcaag tactcagcta atcgattagg ttcaacgata
     2941 agccggtttg ccacaaattt catggtgttt gggttgggcg ttgattcggc gtaaacggtt
     3001 gtaggaacaa aaggcatagg gcagggtttc ggtcgacaaa agtacgaagg aaacagcgca
     3061 ggcagataaa tgtttgtaaa aatcggatgc atgaagcata tcaattttgg cgaaaataat
     3121 tgagaggagc attttacctt tcgctggatg aaatggatat cccgttttct tttgattagt
     3181 ttgcttgttt tgcaggcttg ttcatcggat agagatgtgc atcgtgcatt ttattattgg
     3241 aaaaccgagt tccgatttga tgatgaggat agtgtgcttt tggcggattt gaaaaatgat
     3301 cgcttgtatt tgcgcttgtt tgatgtgagt tgggatgcca ctcagaaaaa agcaatacca
     3361 attggaaaat taagtttcaa ggatgatgtt ccttcctttt tagaagtggt tcctgtagtt
     3421 tacatcacct cggaaacttt gtcagaaata ccagccaaac aactccctga actttctaaa
     3481 aacatctttc agctcatcac acaaacacta tcattgaggc gaatagcttg gaaagagata
     3541 cagcttgatt gtgattggac ggatacaaat aaggaggttt attttacctt attgcgggaa
     3601 ttacgcaagc aagttggtgt agataaacag ctcagttgta ccattcgttt acatcaaata
     3661 aaatatcctg agaaaacggg tattccgcct gtcgatcgtg gaatgttgat gtactacaac
     3721 atgggcaatt tgaatgagcc ggggatgcgg aattccattt atgatccaga tatcgcagct
     3781 cgatatgtta cttatgtgga ggattatcca cttccattgg atgttgcact acccgttttt
     3841 acttggggcg ttcataagaa acaagatggc aatagcactt tagtaaataa tttaaccctt
     3901 cgtgatgttc gaataagtgg attatttagg gaacaggcgt ctggcttgtt tttgcctatg
     3961 caaggatgtt tctttcgagg agattatttt caggaaggtg atgaattgcg ggtagaagaa
     4021 ataaatccag aattgtgtaa aagagcagcc gaacaggtta gtccatttct taaaagcagt
     4081 acttttcacg ttgttttatt tcatttagat tctgtaaaca cagcgcgata tggtcagaaa
     4141 tctttcgaaa ctgtctattc tgttttcgat tagcttttta ttgattcctt gtattcctca
     4201 acccatgatg gggtgtggcg gtggcgatcc gattgacatt atagaaatgt cgcttttttc
     4261 tcctgagatt atatctagtt cccagtttga gccttttttt ctcacgtatg agaattatta
     4321 tggatataac gatggagaaa gaagccaaag taatgagtca acaaatacaa gtgaatggga
     4381 aaattatttt gatcaattaa ttcctaaaga atcagttcaa aacctagtac acaaaagtac
     4441 aattgagcat ttagattcat taattctaat gaccaaaggg aaaatgaatc ctggaatatc
     4501 ggtttatact aaattaatta attcgaaaaa atacaattct ggattaagtt atttgaggaa
     4561 ggcaaaagaa gtagaacatg cttttaccta tgattattat tcgtggaatc ctgcgccatt
     4621 agacacgatc agacaaaaaa caattctgcc tgaattagtg aaaggttgga aggaagaaaa
     4681 agatattcgg ataaaaaatc ggtatgcgtt tcaggtaatt cgggtgaatt atttattggg
     4741 gaattatgcc gatggagtta agttttatga ggaacaggtg aaaatggaac ctgctgatgg
     4801 aagtatgttt tatcgcacgt taggttataa agcagcttgc ttatataaac ttcatcgtta
     4861 tgcagaatca aatttcattt atgctcgcat ttacgatgaa tatgaaccac atcgaataga
     4921 tgctttttct agttttcatc cattagaaga agcagattgg aatgcattaa tggccatggc
     4981 aaaaacgaca cgcgaaaaat gtttgttatg gcatttgttt ggcgtgtatg ctgatccatt
     5041 aaagggaatg cgcgaaatta tcgcactgga tccttcttct aatttattag accttttatt
     5101 ggttcgttcg gtgaatttag cagagatgcg attattgaaa aacgtacctg ctatgagtga
     5161 atatgattat ttatattcag attattcagg tgaagagaca atttcaaacg acgcttttaa
     5221 ttcttggaaa agcatcgatt ctacccgagc aacaaccgaa ttattggatt ttgtagaaaa
     5281 tacagcacgt aatttacctg ttcgaaaaga tgtgtggggc acatcagctg cttatttaaa
     5341 ttggttagct aataaaccag ctgcatgtga tgaatggatt tcaaaaacgg aaaaaggtgc
     5401 tgaagggata attgctcgtc aactattcat tattaaagct ttgcgaatgg ttgagtcgat
     5461 tgatcaaatg cgtgtggagg atgagaataa agtgatgcag aatttgcaag taatttcaac
     5521 ttatggtttg gatggttttc cattgagatc agaagctact aaacgttata ttcgaaacgt
     5581 aatgtctcgg atttataccc gaatgaacga tcctttgatg gccgaattga gtgaaacgaa
     5641 ttattaccaa tttttaaatg attcggcgca ggttcaattg atgattgatt ttatgaaacg
     5701 tccagataag cgcccaattc aaaactattt gttgggattt tatccagtga aaattcagga
     5761 attatatgag gt
//
LOCUS       DAOOQW010000151         5764 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_16888_length_5764_cov_4.859695, whole genome shotgun sequence.
ACCESSION   DAOOQW010000151 DAOOQW010000000
VERSION     DAOOQW010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5764
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16888_length_5764_cov_4.859695"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(133..1128)
                     /locus_tag="PK281_08545"
     CDS             complement(133..1128)
                     /locus_tag="PK281_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49435.1"
                     /translation="MKKYSALLLSGLVIALLGSISSCSEDEPYVKPKLSFAESSMTVN
                     EIDGTIEIEVVLDKPAAEDFTISYELDGDAYDEIRAEEEDELADFEVVGDYGEIEIEK
                     GEDSGIITLQLFADFIIEGNESIEIEITSVDSDKIEITNDDDIEITLEQEQDGVLVVL
                     EWDETYTDVDMDMLLRAVDLGGNASTGPVVWGSVNGSFVSGESIFIPKSIEDVVFGLS
                     YTYYEGTAEPMNFTATFAEAVDGVFEAEEDRQVFQGTYTLANINEWTNFNTTKVVQTF
                     ENIAGVFTNFSEITAPATGSRTISSENATSTFNKTHGGLAEPTNLKLPLKSLKKN"
     gene            complement(1215..3605)
                     /locus_tag="PK281_08550"
     CDS             complement(1215..3605)
                     /locus_tag="PK281_08550"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HOY49436.1"
                     /translation="MGLRLALFFCLFTGFVTLSNAQQTPVDPPRTCATMEQDSVNKLK
                     YPRRSTLDDFEEAIQQRMKEIQAREIASGRKQSTLLSIPIVVHIIHNGEAIGSGTNIS
                     KAQVQAQIDVLNEDFRRKPGTPGFNNSPVGADIEIEFCLSPVDQNGIALSEPGIHRFN
                     GNKSSWTREEIEGGLKSSTIWNPNLFYNIWTVRFGGTSSNLLGYAQFPDQSGLSGLNE
                     SGGSASTDGVVVQYSSFGSADKGSFPVMQAPYNKGRTLSHETGHWLGLRHIWGDGNCA
                     DDFVSDTPPAASASSGCPVGRVSCGATNMVENYMDYTNDACMNIFTIGQKTRIRAVMD
                     LSPRRKSLLAANLCSPIVADIPTPNFTADKQLVLLGGEVNFTDLSTNFPNAWSWTFEG
                     GNPSASIERNPKVKYTIPGIYKVTLSASNSLGTSAPLEIDSYITVSEEGLCGSATNFS
                     ESYTPSTIQLSDYGIYTGYLTGHNSLKSKGISEIFTNALGYEYISGMNITFSHVYSTS
                     EDATVTVVVWNARGPQSAPGSVIERKVVLLKQIQEDIENNRATTIVLDRESPVFGKPF
                     HVGIELNYDGDSLAIQSSLDGESTKATSWIQNEAGVWHPYAIDFGANIAMNIEPIIGM
                     NPSVQVSASKQLIYPGEEVTLNGRGASIFVWSTDDGAVQDYTGPQLIIQPTTSTIVTT
                     NGSGLELCNNVAYTTIYIREDITGTEDDLLSNSIMLYPNPGQGNLNIVMENNFLGDVN
                     LSLVSVIGNEVLQPITMKKGEEKLEHNINTSTLQTGIYFVKVRLEGRTIVKKWFKN"
     gene            complement(3615..4088)
                     /locus_tag="PK281_08555"
     CDS             complement(3615..4088)
                     /locus_tag="PK281_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49437.1"
                     /translation="MKTTYIIILFMVALNFSCSEELKPTPYTYTQIFTGENSKTWKLK
                     FLEETLNGEIIETFTINCASDDKYTFYANPERSYEATTGSKKCSDGEANTITDSWSFN
                     NASATLTMLLPFFTTEGSLPFIVKEAKKNKMELEIFFDEEGTESYRVHFEATDED"
     gene            complement(4090..4866)
                     /locus_tag="PK281_08560"
     CDS             complement(4090..4866)
                     /locus_tag="PK281_08560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49438.1"
                     /translation="MKKVATLFYILGVFAMTNCETERIIFKGPYFVRFTESSRTEKES
                     FSKIIDIEIHQSEPVLEEDITITYSIGGDAREGIDYVILGERGKVEIAQGEYFGNVQI
                     QLINNANNIIRSQEITFTIESVSTDQLQVGQDESAIGKTYTFTIVDDCILGGTYIGQR
                     GSPNVTDITVTSSDCETYRLSNWNINVFSTDTEMDLTFIDNGDNTLTIPQQEEENLSE
                     ELATIRGVGIVDPITRQIIMTITLVDFEDQPEVSFTLIPD"
     gene            complement(4867..>5764)
                     /locus_tag="PK281_08565"
     CDS             complement(4867..>5764)
                     /locus_tag="PK281_08565"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOY49439.1"
                     /translation="VTGISIEVNSNIARASEEEILDFILEDYTEALDKLPVQAGNAGY
                     ASDYALRAALAKYHLYNEDWTKAEQFATNVIASNEFTLEPEYQSLVTEDFTDEAIFEV
                     GYTLSDDPGTDPNIGLNNIFVGRREMIPSNQLLVALDGVNSGDRYLSVSFDSRNLKGN
                     DNGYSVAKYGTADANNNNVVVFRLGEMHLIRAEARANLNKVSGTNSAQEDINALRTRA
                     NAPLVGSVTQAQMLSLIEEERKYELAFEGHRWYDLVRTGRAKAVMTAFSSNWKDAYEL
                     WPIPQREILNNPSLADDQNPGY"
BASE COUNT         1580 a         1226 c         1078 g         1880 t
ORIGIN      
        1 gtactctatt tcactctttc actaaatcgg tttcaatatc tgtttctgaa ggttgttgaa
       61 atgaacagcg attttaaaaa gaaagggagt cattaatgat aatgactccc tttcttttta
      121 aaatttggag cattaatttt tcttcagact ttttaatggg agcttcaaat tggttggctc
      181 tgctaagcct ccatgtgttt tattaaatgt tgatgtagca ttttcagatg aaatggttcg
      241 acttccagtt gcaggcgctg tgatttctga aaaattagta aacacaccag caatgttttc
      301 gaaagtttga acaacttttg tcgtattaaa atttgtccat tcattaatgt ttgcaagcgt
      361 atacgttcct tgaaagactt gtcggtcttc ttccgcttca aagactccat cgacagcttc
      421 tgcaaatgtt gctgtaaagt tcataggctc cgcagttcct tcatagtaag tatagcttaa
      481 gccgaataca acgtcttcaa ttgatttcgg tataaaaata ctttcaccgg aaacaaagct
      541 tccattaaca gatccccaaa caacaggtcc tgttgacgca ttgcccccca gatcgactgc
      601 tctcagcaac atatccatgt caacatcagt gtaggtctca tcccactcca gaacaactaa
      661 gactccatcc tgttcttgtt ctaacgttat ttcaatatca tcatcgttgg taatttcaat
      721 cttatcacta tcaacactgg taatttcaat ctcaatactc tcattgccct caataataaa
      781 atcagcaaat aattgcagcg tgatgattcc actatcttca cctttttcga tttcgatctc
      841 tccataatcg ccaacaactt caaagtcagc caattcatct tcttcctcag ctctgatttc
      901 gtcataagca tctccgtcta attcgtaact gatcgtaaaa tcttctgctg caggtttgtc
      961 tagtacaact tcgatttcaa ttgttccatc aatttcatta actgtcatag aagattcagc
     1021 aaatgaaagt ttaggtttta cgtagggttc atcttcacta caggaagata tggatccaag
     1081 caaagcaatg actaaaccag aaagtaggag cgcgctatat tttttcatgt tcagatgttt
     1141 acacatttta aatttactta aatcttttgg cttttttaag ctctattttc aatatcgttt
     1201 gagcggcatt ggacttaatt cttaaaccat ttttttacaa tcgttcgacc ctccagtcta
     1261 acctttacaa aataaattcc ggtctgaagc gtggaagtgt ttatgttgtg ctctaatttt
     1321 tcctcgcctt tcttcatcgt aatcggttgt aatacttcgt ttcctattac tgaaaccaga
     1381 cttagattaa cgtctccaag aaaattattt tccattacga tattcaaatt accttgtccc
     1441 ggatttggat atagcataat gctgtttgat aataggtcat cttcagtgcc ggtaatatct
     1501 tctctaatgt aaatagtggt gtatgcaaca ttgttacaca attccaatcc cgaaccattg
     1561 gtggtaacaa tggtagaagt ggttggttgt ataattaatt gaggcccggt atagtcttgc
     1621 actgcgccat catcggtact ccaaacaaaa atgctggcgc ctcgtccgtt tagtgttacc
     1681 tcttcgcccg ggtaaattaa ttgttttgat gctgatacct gaaccgatgg attcataccg
     1741 ataatgggct caatgttcat agcgatattg gctccgaagt caatggcgta tggatgccag
     1801 actccagctt cattctgaat ccatgaggtt gctttggtcg actcaccatc caatgaagat
     1861 tgtatggcta atgaatctcc atcataattc aattcgatgc cgacatggaa aggttttccg
     1921 aatactggcg attctctatc aagcacaatt gtggtagcac gattgttttc aatgtcttcc
     1981 tgaatttgtt ttagcaagac aactttcctt tcaataactg agcctggtgc gctctgaggt
     2041 cctcgtgcat tccaaacaac tacagtcacc gttgcgtctt cgctggtaga ataaacatga
     2101 ctgaaggtga tgttcattcc actgatgtat tcgtagccta aggcattggt aaagatttca
     2161 gaaatacctt tgctttttaa actgttgtga cctgtgaggt agccagtgta aattccataa
     2221 tcggataact gaatcgtact gggtgtgtaa gactctgaaa agtttgtggc tgatccacac
     2281 aatccttcct ctgaaaccgt aatgtaacta tcgatctcca atggagcgga agtaccaagc
     2341 gaattgctag ccgataaggt aactttataa attccgggta tcgtatattt tacttttgga
     2401 ttacgttcaa ttgatgcact tggattaccg ccttcaaatg tccagctcca tgcattcgga
     2461 aaattggttg agaggtcggt aaagtttacc tcaccaccta ataaaaccaa ttgtttgtcg
     2521 gcagtaaaat tgggtgtagg tatatccgca acaatcggac tgcagaggtt tgcagccagc
     2581 aaggattttc ttcgtgggct cagatccatc actgcgcgta ttcttgtctt ttgcccaatc
     2641 gtaaaaatat tcatgcaggc atcgttcgta tagtccatgt agttttcaac catattggtt
     2701 gctccacaac taacccggcc aacaggacag cctgagcttg cgcttgctgc tggtggtgta
     2761 tctgacacaa aatcgtcagc acaatttcca tctccccaaa tgtgtcggag cccaagccaa
     2821 tgtcctgttt catgtgataa tgttctgcct ttgttatacg gtgcttgcat aacagggaag
     2881 ctgcctttat ctgcagagcc aaaagaagaa tattgaacga ctacaccatc tgtagatgca
     2941 gaaccaccac tttcatttaa ccctgacaat cccgattgat cggggaactg agcataccct
     3001 aataagttgg aacttgtacc accaaaccgc actgtccaga tgttgtagaa taaatttgga
     3061 ttccagatgg tggatgattt caaacctcct tcaatttctt cgcgtgtcca gcttgatttg
     3121 ttaccgttaa aacgatgaat gccaggctca cttaatgcaa taccgttttg atccacaggc
     3181 gacaaacaaa attcgatttc aatatctgcg cctacgggac tattattgaa tcctggtgtt
     3241 cctggtttcc ttctgaagtc ttcattcaag acatcaattt gtgcttgtac ctgagcctta
     3301 ctaatatttg tacctgagcc aatggcttca ccattatgaa tgatgtgtac tacaattgga
     3361 atagaaagaa gagtgctttg ttttctacct gaagctattt cacgcgcttg aatttctttc
     3421 attctttgct gaatggcttc ctcgaaatca tcaagcgtgc tgcgtctcgg atactttaat
     3481 ttgttcacac tgtcttgctc catggtagca caggtgcgtg gaggatcaac aggagtttgc
     3541 tgtgcattgg atagcgtaac aaatccggta aacaaacaaa aaaatagagc aagacgtaat
     3601 cccataatat tcacttaatc ttcatctgta gcttcgaagt ggactcggta actttccgtt
     3661 ccttcttcat caaagaaaat ttccaattcc attttatttt tcttcgcttc ctttacaatg
     3721 aagggcaaac ttccttctgt agtaaagaat ggaagtaaca tggtaagtgt tgccgatgca
     3781 ttgttgaagg accatgaatc agttatagta tttgcttctc catcgctgca tttttttgac
     3841 ccggttgtag cttcatacga acgttcagga ttcgcgtaaa aagtatattt atcatcggat
     3901 gcacaattga tagtaaatgt ttcaattatt tctccattga gtgtttcttc caaaaatttt
     3961 aatttccagg tcttgctatt ctcacctgta aaaatttgag tgtacgtata aggagtaggt
     4021 ttaagttctt ctgagcagga gaagtttaag gctaccataa agagaataat gatgtatgta
     4081 gttttcatat caatccggta tgagggtgaa tgatacttca ggctggtctt caaaatcaac
     4141 gagggttatg gtcataataa tttgacgtgt tattggatca acgataccaa ctccgcgaat
     4201 ggttgccaat tcttcagaaa gattttcttc ttcttgttga ggaatggtta aggtattatc
     4261 gccattatca ataaacgtta aatccatttc ggtatctgta ctaaacacat tgatattcca
     4321 attggagagt cggtatgttt cgcaatccga acttgtaacg gtaatgtcgg taacgtttgg
     4381 cgatccgcgt tgtccgatat aagtcccacc tagtatgcaa tcatcaacaa tggtgaaggt
     4441 gtaggtttta ccaatggcac tttcatcttg ccctacctgc agttgatcgg tactaaccga
     4501 ttcaatggta aaggtaattt cctgactccg gatgatatta ttggcattgt taatcaattg
     4561 aatttgaaca ttgccaaagt attcaccttg ggcaatctct acttttccac gttcaccaag
     4621 aataacatag tcaattcctt cgcgtgcatc gccaccaatg gaataggtaa tggttatatc
     4681 ttcttccaat acaggttctg actgatggat ttcaatatca ataattttgc tgaagctctc
     4741 tttttcagtt cgtgatgatt ctgtaaaccg aacgaagtaa ggacctttaa agatgatgcg
     4801 ttctgtttcg caatttgtca ttgcaaacac accaaggata tagaataagg ttgctacttt
     4861 tttcatttag tatccaggat tttgatcgtc tgccaaagat ggattattca aaatttcacg
     4921 ttgcggaatg ggccacaatt cataagcatc tttccaattg gaagaaaatg cagtcatcac
     4981 cgctttcgcc cgacccgttc taaccagatc ataccaacga tgtccttcga aagccaattc
     5041 atattttctt tcttcttcaa taagacttaa catctgggct tgtgttacgg atccaaccag
     5101 tggagcgttt gcgcgagtgc gcagtgcatt gatgtcttcc tgcgcactat ttgttccgga
     5161 taccttattg agattcgctc tcgcctctgc gcgaatgaga tgcatttctc caagtcgaaa
     5221 gacaactaca ttgttgttat tcgcatccgc tgttccatat ttggcaaccg agtagccgtt
     5281 gtcatttccc tttagatttc tggagtcaaa ggaaacagaa agataccggt ctcctgaatt
     5341 gactccatct aatgcaacca ataattgatt ggatggaatc atttctcttc tgcctacaaa
     5401 aatattattc aacccgatat tcggatccgt cccaggatca tcagaaagag tatagcccac
     5461 ttcaaagatc gcttcgtccg taaaatcttc cgtcaccaaa gattgatatt ctggttcaag
     5521 tgtaaattca tttgacgcga taacattggt tgcgaattgc tcagcctttg tccagtcttc
     5581 attgtataaa tgatattttg ccagagcggc acgtaatgca taatcactgg cataacccgc
     5641 gttacctgct tgtaccggca gcttatccaa tgcttcagta taatcttcta atataaaatc
     5701 cagaatttct tcttctgatg cgcgggcgat gttgctgttt acttcaattg atattcccgt
     5761 tact
//
LOCUS       DAOOQW010000152         5760 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_16896_length_5760_cov_4.641192, whole genome shotgun sequence.
ACCESSION   DAOOQW010000152 DAOOQW010000000
VERSION     DAOOQW010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5760
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16896_length_5760_cov_4.641192"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1153
                     /locus_tag="PK281_08570"
     CDS             <1..1153
                     /locus_tag="PK281_08570"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOY49440.1"
                     /translation="KVMKGDHLLTINPDMIKAVADRVAAALNQAKANLANARARENQV
                     KASFLNIEISYNRSKKLYDQKAISDAEFDAAKAQYEGSKADLEAARQTVIGADFSVRS
                     AEASLKEANDNLARTRIYAPTDGTVSKLNVELGERVVGTAQMSGTELLRIANLNEMEV
                     RVDVNENDIVRIHLNDTSDVDVDAYADRKFKGIVTEISNSSNNSSVAVNSADQVTSFT
                     VKIRILRESYDDLMDPEHPYLSPFRPGMSATVDIRTHSEVNVLTVPIMSVTVRTLDEL
                     KNKKEGNREDEKPVAEEEGESTTKKEDTKTSTEEDVKEIVFVLEAGKVKPVEVKTGIQ
                     DNNYIQILSGLKEGEEVISGPYSAISKILKDGTEVEKTTTEKLNQRKEE"
     gene            1155..1874
                     /gene="tsaB"
                     /locus_tag="PK281_08575"
     CDS             1155..1874
                     /gene="tsaB"
                     /locus_tag="PK281_08575"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOY49441.1"
                     /translation="MSGIRILQIETATTTCSVACAEDGNLIALKSVSDGYKHAENVFG
                     LIREVLAEAKWESRDLNAIAISAGPGSYTGLRIGVSAAKGLCYSLDIPLIASSTLFTL
                     ANSFKRQFPEVDTYLSPMIDARRMEVYTAIYSADLHAIKSDHPLVVTDPDVIPELNDQ
                     VITFFGDGAGKCAEVLGVHPNARFAVGIELSAQDMSASAYESYQLGAFADLAYFEPEY
                     LKPYHGTQPLPSTNFGSEIKS"
     gene            1871..2806
                     /gene="rsgA"
                     /locus_tag="PK281_08580"
     CDS             1871..2806
                     /gene="rsgA"
                     /locus_tag="PK281_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009389305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HOY49442.1"
                     /translation="MKGLVIRSTGSFCTVKTDDGVIRECKVRGVFRMKGLRTTNPIAV
                     GDRVTVEESVGGDYYVITEIDPRENYIIRRSINLSKEAHILAANVGHAFVIATVSSPR
                     TSYGFIDRMLINCEAYHIPVSVVVNKCDLYESDADLDKLAELQDIYTQAGYEVLLVSA
                     STGFQVDELRKRMDGKINLFIGHSGVGKSTLINALNPSLELKTGDISSAHSKGKHTTT
                     FAELFGLWEDTWIIDTPGVKEFGIVDMKKEEIGDYFPEIRALSEHCRFSNCMHENEPG
                     CAVKEAAEDGRFEVHRYNSYLGLLHSEELSKESWD"
     gene            2810..3262
                     /gene="dtd"
                     /locus_tag="PK281_08585"
     CDS             2810..3262
                     /gene="dtd"
                     /locus_tag="PK281_08585"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016650218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOY49443.1"
                     /translation="MRVVVQRVTSASVSIDDTVYNKIRQGLLLLVGIEELDTVEDLVW
                     LAAKIVNMRIFSDDNGLMNKSVADVFGEILVISQFTLFASTKKGNRPSFIRAAKPEYA
                     TKLYEMFCAQLSQELSRPVKTGVFGADMQIALMNDGPVTILIDSKNRE"
     gene            3307..5214
                     /locus_tag="PK281_08590"
     CDS             3307..5214
                     /locus_tag="PK281_08590"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY49444.1"
                     /translation="MKRKVLVLLSVITCLVVALWPNQSAHSNGGGAPAGYTGSPNEFS
                     GRTCSAGGSCHSGGTTAQTGWVTSTVPACGYTPGQTYSVTVFVTSPGRTKFGFSASPQ
                     KANATQAGTLIVGTGMQLNGSGKYITHTSAGTTQTGTNSRTWTFQWTAPAAGTGTVTF
                     YAAMNATNSSNSSAGDMIYSSNLVLAEAPSTLSINNSGGTSYCQSSPVTLTSNISGGN
                     VWTKDGNPIGTQNSVVASGSGTYELTNTTGACVQTASVTLTAVAGAPVVSDIQIGSQG
                     IDLCSGQSTTLTATGSGVTWLPGGQTTNTITVSTAGTYSYSLTNVCGTSTSNSVGVTV
                     TPLPTTPVIQTPDGLSVCPSASIQLTAVSSNNVVWSPGGETTASIQVSTGGTYSVVSN
                     NSCGSSSPIQVTVEELSLPETPEITTAGDITEACEGSTITLTAANIGSNQALWLPGDG
                     TNSTFEASQSGTYSVTYTNVCGTSESTDIDLTFNPLPETPSIFLNTDLDLEATIDASF
                     YEWYLNGTLIDGENDQVLTPSGVGFYTVKAISGDDCVSELSVAFEVTALGLTNILGTN
                     IRIFPNPSEGNIQLTGITKQTSFQVYDATGKSVKNFVVSANSQQSIDLNLPSGLYFIR
                     CDKWNSTLVVE"
BASE COUNT         1737 a         1073 c         1285 g         1665 t
ORIGIN      
        1 aaaagtgatg aagggcgatc atttgctcac catcaatccc gatatgatta aagctgttgc
       61 cgatcgtgtt gctgcggcat taaatcaagc aaaagccaat ctggctaatg cacgtgcgcg
      121 ggaaaatcaa gttaaagcga gtttcttaaa cattgaaatc agctacaaca gaagtaaaaa
      181 attgtacgat caaaaagcaa tttcggatgc cgaatttgat gctgcaaaag ctcaatatga
      241 aggttcaaaa gccgatttag aagctgctcg tcaaaccgtt attggtgccg attttagtgt
      301 gagaagcgca gaagcaagtt tgaaagaagc caacgataat ctagcaagaa cccgtattta
      361 tgctccaacc gatggaaccg tttcgaaatt gaatgttgaa ttaggtgagc gcgttgttgg
      421 aactgctcaa atgagtggta cagaattgct tcgcattgcc aacctcaacg aaatggaagt
      481 gcgggttgat gtgaatgaaa acgatattgt tcgtattcac ctcaacgata cgagtgatgt
      541 agatgtagat gcttacgccg atcgtaaatt caaaggtatt gttacggaga tttcgaattc
      601 atctaacaac tcaagtgttg cagttaatag tgctgatcaa gtaacaagct ttaccgttaa
      661 aattcgaatt cttagagaat cgtacgatga tttgatggat cctgaacatc cgtatttatc
      721 accattccgt ccgggcatgt cggcaaccgt tgatattcgt acccattcgg aagtgaatgt
      781 gcttacagtt cctattatgt cggtgactgt tcgaacatta gatgaactca aaaacaaaaa
      841 agagggaaat cgtgaagatg aaaaacctgt ggcagaagag gaaggagaaa gcacaactaa
      901 gaaagaagac acaaaaacca gtacagaaga ggacgtgaag gagattgtgt ttgttttgga
      961 agctggaaaa gttaaacctg ttgaagttaa aacgggtatt caggacaata actacattca
     1021 gattttaagt ggtttgaagg aaggagaaga agtaatttca ggaccttatt cggctatttc
     1081 gaaaattctg aaagatggta ccgaagtaga aaaaacgacc acagaaaagc ttaaccaacg
     1141 caaggaagaa taaggtgagt ggcattcgca tacttcaaat cgagacagct accacaacat
     1201 gttcggttgc ttgcgcagaa gatggaaatc tgattgcttt aaaatcagtt agtgacgggt
     1261 ataagcatgc tgagaacgtg tttggattga ttcgagaagt tcttgcagaa gccaaatggg
     1321 aaagccgtga cttgaatgcc attgctatat ctgccggacc tggatcttat acaggattgc
     1381 gcataggtgt ttcagcagct aaaggattgt gttattcgtt ggacattcca ctcattgcat
     1441 cctctactct tttcacgctt gcgaattcat tcaaacgtca gtttcctgag gtcgatactt
     1501 atttgtcgcc tatgattgat gctcgtcgga tggaagtata tacagctatc tattcggccg
     1561 atttgcatgc cattaagtcg gatcatccac tagttgttac cgatccagat gtgattcctg
     1621 aacttaacga tcaggtaatt acttttttcg gtgatggtgc aggaaagtgt gctgaagtat
     1681 taggtgttca tccaaatgcc cgatttgcag tagggattga actttcggct caagacatga
     1741 gtgcttcagc atacgaatca taccaattag gcgcatttgc tgatttggcc tatttcgaac
     1801 ctgaatacct caaaccatat catggtaccc aaccgcttcc atcaacaaat tttgggagtg
     1861 aaataaaatc atgaaagggc ttgttatccg atctacaggt agcttctgca cggtaaaaac
     1921 cgacgatgga gttattcgtg aatgcaaggt tcgaggtgtt ttccgaatga aaggattacg
     1981 tacgaccaat ccgattgctg ttggagatcg tgttaccgtg gaagaatctg ttggtggtga
     2041 ttattatgtg atcactgaaa tagatccgcg ggagaattac atcattcgtc gttccattaa
     2101 tttgagtaag gaagctcata ttttggccgc caatgtggga catgcatttg tgatcgccac
     2161 agttagttca cctcgtactt cgtatggttt tattgatcgg atgttgatta actgtgaagc
     2221 ataccacatt ccggtttcgg tcgtagtgaa taagtgtgat ttatatgaat cggatgctga
     2281 tttagataag cttgccgaat tacaagatat ctatacgcaa gctggttatg aagtccttct
     2341 agtttccgct agcacaggct ttcaagtgga tgaattacgt aaacggatgg atggtaaaat
     2401 caatctgttt ataggtcatt cgggtgttgg aaaatcaact ttaataaatg ccttgaatcc
     2461 atcactggaa ttaaaaacgg gtgatatttc atctgctcac agcaaaggaa aacacaccac
     2521 cacatttgca gagttgtttg gattatggga agacacttgg attatcgaca cgcccggagt
     2581 gaaagaattc ggaattgtgg atatgaagaa agaggaaatt ggtgattatt tcccggagat
     2641 acgagcatta tcggagcatt gtcgtttctc gaattgcatg cacgaaaatg agcccggatg
     2701 tgcggtgaaa gaagctgccg aagatggtcg attcgaagtt catcgttaca attcttacct
     2761 tggcctgctc cattctgaag aactaagtaa agaatcctgg gattaaagca tgagagttgt
     2821 cgttcagcgt gttacaagcg cttccgtcag cattgacgat accgtttaca ataaaatccg
     2881 acaaggatta ttgctattag ttggcataga agagctcgat acggtggaag atttggtatg
     2941 gctagcagcc aaaatcgtaa acatgcgcat atttagtgac gataatggct tgatgaataa
     3001 aagcgtagcg gatgtttttg gggagattct agtaattagt cagtttacct tattcgctag
     3061 cacaaaaaag ggaaatcggc cttcattcat tcgagcagca aaacctgaat atgcaacgaa
     3121 attgtatgaa atgttttgtg ctcaattaag tcaggaatta agcagacccg ttaaaactgg
     3181 ggtgtttgga gcagatatgc aaatagcatt aatgaatgat gggccagtta ctatcctaat
     3241 tgatagtaag aacagagaat agaatttggt catttttttc tactttcatg cggaaattcc
     3301 tgactcatga aaagaaaagt actcgtatta cttagtgtta ttacttgttt ggttgttgct
     3361 ttatggccaa accaatcggc gcattccaat ggtggaggcg cacctgctgg ttataccggt
     3421 tcacctaatg aattttccgg acgtacttgt tcagctggag gaagctgtca cagtggagga
     3481 acaacagctc aaactggctg ggtaacttca acagttccag catgcggcta tacaccggga
     3541 caaacttatt ctgtgactgt atttgttaca tcacccggaa gaacaaaatt tggatttagt
     3601 gcttctccac aaaaagcaaa tgctacgcaa gctggtactt tgatcgttgg tacaggcatg
     3661 caattaaacg gaagtggaaa atacatcact catacaagtg ctggaacgac tcaaactgga
     3721 accaactctc gcacctggac tttccagtgg acagccccag cagctggaac gggaacagtt
     3781 actttttacg cagctatgaa tgctaccaac agcagcaaca gcagtgcggg tgacatgatt
     3841 tatagttcaa acttggttct tgcagaagca ccatcaaccc taagtatcaa taacagtggt
     3901 ggaacaagct attgtcaaag ttcacctgtt acacttactt ccaatatctc aggaggaaat
     3961 gtttggacaa aagatggcaa tcccattgga actcaaaatt cggttgttgc aagtggaagt
     4021 ggaacgtatg agttaaccaa tactactggt gcttgtgttc aaactgctag tgtaacgctt
     4081 actgctgttg caggagctcc tgttgtatct gatattcaaa ttggatctca aggtattgat
     4141 ttgtgtagtg gtcaatcaac taccttaaca gctactggct ctggagttac atggctaccc
     4201 ggcggacaaa caaccaatac aattacagtt agcacagctg gaacttattc gtattcatta
     4261 acgaatgtat gtgggacgag tacttctaat tcggttggag taactgtaac accattacct
     4321 acaacaccag ttattcaaac tccagatgga ttgtcggttt gtccttctgc tagtattcaa
     4381 ttaactgcag tttcttctaa taatgttgtt tggtctccag gtggagaaac aactgcgagt
     4441 atacaagtaa gtacaggtgg tacttattcg gtagtatcga ataatagttg tggtagttca
     4501 agtcccattc aggtgactgt tgaagaactt tcactccctg agacaccaga aattacaact
     4561 gctggcgata taacagaagc ttgtgaagga tcaacaataa cactaacagc agctaatatt
     4621 ggaagcaatc aagctctttg gctcccaggt gatggaacaa actccacatt tgaagcatca
     4681 caatctggta cctattcggt tacatataca aatgtgtgcg gtacatctga aagtacagat
     4741 atcgatttaa cattcaatcc actaccagaa acaccaagta tctttttgaa tacagattta
     4801 gatttagaag caacaattga tgcttcattt tatgaatggt atctgaatgg aactttgatc
     4861 gatggtgaga atgatcaagt attaactcca agtggagtag gattttatac agttaaagct
     4921 atttcgggtg atgattgtgt aagtgaactc tcagttgcat ttgaggtaac agcattaggt
     4981 ttgaccaata ttttaggaac gaacattcgt attttcccaa atcctagcga agggaatatt
     5041 cagttaactg gaatcacaaa acaaacttcg tttcaagttt acgatgcaac tggtaaaagc
     5101 gtaaaaaact ttgttgtatc agccaattcg caacaatcga tcgacctgaa tcttccatcg
     5161 ggtttgtatt tcatccgttg tgataaatgg aattctacat tagtagtgga ataaagccct
     5221 tggttctgga tttttaatca gttgcattaa attactaggt gctgccccct aaaaagggca
     5281 gcattttttt tgcattctag tatttagcga gcattaactt gtgtcgaatt taccactttg
     5341 tcaattatta attaattcta ttgccacata ttcattctgt caatttttca cctgtattat
     5401 ttgcatagtt ctatgccaat ctatatgttt gcagtaatta gttcatttta ttgtaggatg
     5461 ttgaaacggc agacaaaccc tcttgtctcg ggggtgagga ataacaggac aaacgatgtt
     5521 taacgggttg accactccat tctgcttgca gaatctgaac attggctaac tgcctcgtgg
     5581 aggttcgagt ccttctccta cagcgaatcc cctaataccc agccccgacg tttgtgatgg
     5641 ttcgtcgggg ctattttttt gggaattact ctgagtattt atcaagcaaa taaagcagtg
     5701 cggcaataga agcggcacct aattccaatt ctcgcttgtt aaccttatcg aaggtgtcgg
//
LOCUS       DAOOQW010000153         5757 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_16906_length_5757_cov_4.961592, whole genome shotgun sequence.
ACCESSION   DAOOQW010000153 DAOOQW010000000
VERSION     DAOOQW010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5757
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16906_length_5757_cov_4.961592"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1045)
                     /locus_tag="PK281_08595"
     CDS             complement(<1..1045)
                     /locus_tag="PK281_08595"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOY49445.1"
                     /translation="MKKSGKLYGIHLLNDISGSPLVFSMVLADYVEDGYDVHLYTSNS
                     KGFLSDIEGVNYHHLPYSWSPKKLITLINFLYVQFYLFCSLLVKLKKNDTVHVNTLLP
                     FGALIAAKIRGVHTIQHMHEVSVKPASLFRFLCFVSEHCTREMIFVSHYLKSCFHFNK
                     PLQRVIHNRLDADFLNEVKKHGKVNPTGPFTVLMLCSLKEYKGVYVFTRLAADFPSVR
                     FRLMLNASEADMQEFIEKEKPSTNCEILSPTRNTHSAYQQADLVMNLSLPDQWIETFG
                     MTILEAKAYGIKVIAPPVGGPAEIVSVSEDGWLIDSRNYESLRLKLQELTHDFTRIPH
                     NMNTISNTSYTDLN"
     gene            complement(1042..2364)
                     /locus_tag="PK281_08600"
     CDS             complement(1042..2364)
                     /locus_tag="PK281_08600"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOY49446.1"
                     /translation="MINTSTPKITVFASAYTINPFKGSEDGMGWNFVLQIARNCHVIA
                     VTRKNNRPAIEKYQQEHPELSDLYKRIEFRYYDWPKRFLFWKKGPVLSLIYYYFWQLG
                     LALHFRSLKNQVDVVHNLNFHNDWTPSFLWIWRKPFVWGPVGHHPRIPKGFIRPFYSN
                     RNLIMDRFLWTLKNIFWYLDPFLKITRNSADHIFCMHQEAANKLGLKNKYSILPSVAS
                     EEVDKPIETDEFIVLSAGRFVGLKGFDITIEAFHQFYIQLKDSEQRKAKLILVGSGPE
                     KHKLKALALSLGISAQLEIIEWIPRDELKKIYQTTSVFLFPSHEGAGMVVAEALSYGV
                     PVLCWDNCGPGRFIHPDSTLKIAYDPNSDWKNAFADQLLRLKHDATFYQHERNLARQR
                     FLSTFLWDNRADELYSVYKDVAGKTQDSFANSEQNDTVFSLTGNSISQ"
     gene            complement(2361..3065)
                     /locus_tag="PK281_08605"
     CDS             complement(2361..3065)
                     /locus_tag="PK281_08605"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOY49447.1"
                     /translation="MKHLYTRLIQVRNPDFKMHEAVTGKMLWSFGIQTLCQLLRGLKV
                     ILRGRSPKMMMLGTSVKFRYFSSIQYGRMLKAGDHVLFSALGIEGIQLGNNVSIGSYS
                     QIIASTSLNQPGIGIQIGDRVGMGEFAYLGGGGGLKIGSDCIIGQYFSCHPENHVFSN
                     PEELIRLQGVTRKGIEIGSGCWIGSKVTILDGAHIGNHCVVAAGAVVTGHFPDYAVIG
                     GVPARILKQRDQQSAA"
     gene            complement(3022..5487)
                     /locus_tag="PK281_08610"
     CDS             complement(3022..5487)
                     /locus_tag="PK281_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49448.1"
                     /translation="MENSVNLAFTMSDASIRNSVNEPDKITAEFNSTHPDEIHMLLKF
                     GLIQTANPSISAIVHYICNTDGTIRWMWPAKSSKPNFIHFYTASNFKSRIFVWAAKSA
                     FACGLGAFFKQGKLELKGFEFTSLTNWAVFTGTVGPNRKAILRNQNNSGDVEYRKLPF
                     SAEAKQNLIQENRALLKLKSSQAERFFDYPFSTSLNEDEVFGQTSLVGNHAVSVPLPN
                     QLDLIGFTNWLSDGLQQSAPDISISTFQTQKFDPEFCNNLEQLKEFCTNNPLSVSAPV
                     HGDFTPWNCRMNDSRLQIFDWEMFMEKGIPLEDLFHYCYQQAILVDRISFAQLRNRIQ
                     DMLTNPAVDAFLKNNKLHPRLLEMHYLNRLCTYYLNVYSNQDKWHVQINWLIDIWAQS
                     VRYLLAVQDSTVRKTVLSDFQNAISGLEYALLKFNLSELALTPATSDIDLCTNRDAAN
                     QMIQLLQNHPWVNVVKIKNLIKMLQLEVVLLDGSRIDLDLIFQFKRHHIVYLDAEKVL
                     ANRIISPNGISNASPECNRDYVLRFYTLNGSAVPEKHLFWTHHQLSNEEQKQLIQNIR
                     VSPENSGFSKIKNRIGYWFEIVQNMRSEKGFLITFSGVDGAGKSTIIEAVKTELQKKH
                     RRRIIVIRHRPSILPIISAWKYGKKGAEERSVSTLPRQGKNSSKLSSFIRFAYYLCDY
                     IIGQWIIHFRYVRKNTIVLYDRYYFDFINDSRRSNIALNPSFTEKFYFLIKKPEFNYF
                     LWAAPDVILKRKQELDKDTIITLTNQYTELFARLQNADKKHIYRCIENVEIGETVESI
                     MRNVVGNTSNETPIHPTHSSTKS"
     gene            complement(5487..>5757)
                     /locus_tag="PK281_08615"
     CDS             complement(5487..>5757)
                     /locus_tag="PK281_08615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49449.1"
                     /translation="TLKATVTEKVNDKKLMILGMFIPILINSFTEFGIFGETNYGILF
                     YQLIIFYITMQLPNQLSLRERVKLKIRQKKHTWLQSKQPNQLISS"
BASE COUNT         1898 a         1076 c         1009 g         1774 t
ORIGIN      
        1 cgttcaaatc ggtataagaa gtattcgaaa tcgtattcat gttgtgtggt atgcgagtaa
       61 aatcgtgagt taattcctga agcttgagtc gaagagattc ataattacgg gaatcaatta
      121 accaaccatc ttcggataca gaaactattt cagcaggacc accaactggc ggagcaataa
      181 cctttattcc gtatgctttc gcttctagaa ttgtcatccc aaaagtttca atccattgat
      241 caggtaaact caaattcatc actaaatcgg cttgttgata agcagaatgc gtattacgag
      301 ttggtgaaag aatttcacaa ttagtgcttg gtttttcttt ctcaataaat tcttgcatat
      361 cggcttctga tgcatttagc atgagtcgga agcgaacaga aggaaaatct gcagctaaac
      421 gtgtaaatac ataaacacct ttgtattcct ttaaactaca cagcattaac acagtgaaag
      481 gtccggtagg attaaccttt ccgtgctttt ttacttcgtt aagaaaatcc gcatcaaggc
      541 gattatgaat tacacgttgt aatggtttat tgaaatgaaa acagcttttt aaataatgag
      601 aaacaaaaat catttctcgt gtacaatgtt ccgaaacaaa acaaaggaaa cggaataatg
      661 aagccggttt cacacttact tcatgcatat gctgaatagt atgaactcca cgaattttag
      721 ctgctattaa tgcaccaaat ggtagtaatg tatttacatg aactgtatcg tttttcttaa
      781 gttttactaa gagagaacag aataaataaa actgaacgta aagaaaatta attaacgtaa
      841 ttaatttctt cggactccag gaataaggaa gatggtgata atttacccct tcaatatccg
      901 acaggaaacc tttactatta gacgtataca agtgaacatc atatccatct tctacataat
      961 cagctaacac catagaaaaa actaatgggc taccactgat atcattaagt aaatgaattc
     1021 cgtatagttt tcctgatttt ttcattgtga aatagaattt ccagttaaac taaaaacggt
     1081 gtcattttgc tccgagtttg caaatgaatc ttgtgttttt ccagcaacat ctttgtatac
     1141 agaatagagc tcatctgccc ggttatccca aaggaaagtg cttaaaaatc gctgacgagc
     1201 aagattccgt tcgtgctgat aaaatgttgc atcatgtttc aatcgtagta attgatctgc
     1261 aaatgcattt ttccaatctg aatttggatc atacgctatt ttcaaagttg aatctggatg
     1321 tatgaaacgt ccagggccgc agttatccca acataataca ggaacaccat agcttagtgc
     1381 ttctgctact accattccag ctccttcgtg tgatgggaac agaaatacgg aagttgtttg
     1441 ataaatcttt tttaattcat ctcgaggaat ccactcaata atttcgagtt gagcagaaat
     1501 acctaaagaa agcgctaatg cttttagttt gtgcttttct ggaccacttc cgaccaagat
     1561 taatttggct ttgcgttgtt ccgaatcttt cagttgaata tagaattgat gaaatgcttc
     1621 aattgtaata tcgaaacctt ttaatccaac aaatcgcccg gcagataaaa caataaactc
     1681 atctgtttca ataggtttat caacctcttc ggaagctacg gaaggtagaa tggagtattt
     1741 atttttcaat cccaatttat tcgcagcttc ttgatgcata cagaaaatat gatcagccga
     1801 attgcgcgtt attttaagga atggatctaa ataccagaat atatttttta aggtccaaag
     1861 aaatcgatcc ataattaaat tacggttcga ataaaatggg cgaataaatc ctttaggaat
     1921 tcgaggatga tgtccaactg gtccccaaac aaatggtttc cgccaaatcc aaagaaaact
     1981 aggtgtccaa tcgttatgga aattcagatt atggacaaca tccacttgat ttttaagtga
     2041 acgaaaatgt agagccaatc caagctgcca gaaataataa tagatcagac ttaatactgg
     2101 accttttttc caaaataaga aacgcttagg ccaatcgtaa taacgaaact caatacgttt
     2161 atataaatca cttaattcag gatgttcttg ctgatatttc tcaattgcag gacgattatt
     2221 cttgcgcgtt actgcaatta catgacaatt acgggcaatt tgcagaacaa aattccatcc
     2281 cattccatct tcagatcctt taaaaggatt gatagtatag gcagaagcaa acacggtaat
     2341 tttaggagta gaggtgttta tcatgcagca gattgttgat cgcgttgttt taatattcga
     2401 gctggtacgc cgcctattac agcataatca ggaaaatgtc cagtaacaac tgccccagct
     2461 gcaacaacac aatgatttcc tatatgagca ccatctaaaa tggtcacttt agagccaatc
     2521 caacagccag aaccaatttc aattccttta cgagtaactc cttgcaaacg aattaattcc
     2581 tctggatttg agaaaacatg attttcggga tgacaagaga agtattgacc aatgatgcaa
     2641 tcggaaccga ttttcaatcc acctccacca cctaaatagg cgaattctcc cattccaact
     2701 cgatcaccaa tttgaattcc aataccaggt tgatttaaag aagtagatgc gattatctga
     2761 ctataagagc caatactcac attatttcct agttgaatac cctcaatacc tagggctgaa
     2821 aacaaaacat ggtcgcctgc ctttagcata cgaccatact ggatggatga aaaataacgg
     2881 aatttaacac ttgtcccgag catcatcatt ttaggagaac gccctctgag tatgactttt
     2941 agccctctca gcaactgaca caaagtttga attccaaaac tccaaagcat tttgccagtt
     3001 acggcttcat gcattttgaa gtcaggattt cgtacttgaa tgagtcgggt gtataggtgt
     3061 ttcattggat gtgttaccca ccacattacg catgatggat tccactgttt cgccaatttc
     3121 aacgttttct atacatcgat aaatatgctt tttgtcggca ttctgcagac gagcaaataa
     3181 ttcggtatac tgatttgtca gcgttataat cgtgtcttta tcgagctctt gtttacgctt
     3241 caatatcacg tctggagcgg cccacagaaa ataattgaat tcaggtttct tgattaagaa
     3301 atagaatttt tctgtaaatg atggatttaa agcgatgtta cttcttcgac tatcatttat
     3361 aaaatcgaag taataccgat catatagcac aatggtattc tttcttacat atcgaaaatg
     3421 aataatccac tgtccaatta tataatcaca taaataatag gcaaaacgaa tgaatgaaga
     3481 aagcttactc gaattttttc cttgacgagg caaagtcgaa acagaacgtt cctcagctcc
     3541 tttttttccg tatttccaag cactaatgat aggtaatata gaaggacgat gacgaattac
     3601 tatgatgcga cgacgatgtt tcttttgcag ttcggtttta acagcttcaa taatggttga
     3661 ttttcctgca ccatcaacgc cgctaaatgt aattaggaat cctttttcgc tacgcatatt
     3721 ctgaactatt tcgaaccaat atccgattct atttttgatt tttgagaagc ccgaattctc
     3781 aggacttaca cgtatatttt gaatgagttg tttctgctcc tcattactga gttgatgatg
     3841 tgtccaaaat aagtgttttt cgggcacagc agagccattt aatgtataaa accgaagtac
     3901 ataatcccga ttgcattcag gagaagcatt ggagattcca ttgggagaaa taattcgatt
     3961 tgcaagtact ttttccgcat ccaaataaac gatatgatgc cttttaaact gaaaaattaa
     4021 atctagatca attcgactac catcaagaag aactacttca agctgtagca ttttgattaa
     4081 attcttgatt ttgacaacat tcacccaagg atgattttgc aacagttgaa tcatttggtt
     4141 agctgcatct cgattcgtac acaaatcaat atcagaagta gctggagtta atgctaattc
     4201 agataaattg aattttaaga gtgcatattc taaaccagaa atagcgtttt gaaaatcgct
     4261 taataccgtt tttcgaacgg ttgaatcctg aactgcaagt aaatatcgaa ccgattgagc
     4321 ccaaatatct atgagccaat taatttgaac atgccattta tcctgatttg aataaacgtt
     4381 taagtaatag gtacataaac gattcaaata atgcatctca agtaatctag gatgcagttt
     4441 gttatttttc aaaaatgcat ctacagcagg attggtgagc atatcctgaa ttcgatttcg
     4501 taattgagca aaagaaatac gatcaactaa aatggcttgt tgatagcaat aatgaaaaag
     4561 atcctccaaa ggaattccct tttccataaa catttcccaa tcgaaaatct gcaaacgact
     4621 gtcattcatc cggcaattcc agggagtaaa atcaccatgc acaggtgctg aaaccgagag
     4681 tggattattc gtacagaatt cttttaattg ttcaagatta ttacaaaatt caggatcgaa
     4741 tttttgagtt tgaaaagttg aaatcgaaat atctggagct gattgttgca aaccatctga
     4801 aagccaattg gtaaatccaa ttaaatcgag ttgattgggc aagggaacag aaacagcgtg
     4861 atttccaact aaagatgttt gtccaaatac ttcatcctca tttaatgaag tgctaaatgg
     4921 atagtcgaaa aatctctctg cttgagaaga tttcaatttc agtaaagcac ggttttcttg
     4981 aatgaggttt tgctttgctt cagctgaaaa gggtaatttc ctatattcta catcaccaga
     5041 attattttga ttgcgcaaga tagctttgcg gtttgggcca acggttccag taaatactgc
     5101 ccaattcgtt aaagaagtaa attcaaaacc ttttaattcc aatttcccct gcttgaaaaa
     5161 agcacctaat ccacatgcaa atgcagattt agcagcccat acaaaaattc gacttttgaa
     5221 atttgatgcc gtgtaaaagt ggatgaaatt aggttttgaa gatttagctg gccacatcca
     5281 acgaatggtt ccgtcggtat tacagatata atgaactata gctgaaattg agggattagc
     5341 tgtttgaatt aatccaaact tcaacaacat gtgaatttca tccggatgtg ttgaattaaa
     5401 ttcagcagta atcttatccg gctcattaac agaatttcgg atggatgcat cggacatagt
     5461 aaaggctaaa ttaaccgaat tttccatcag gaagaaataa gctgattagg ctgtttagat
     5521 tgaagccaag tatgtttctt ttgcctaatc ttaagcttca cccgttcgcg aagactcaat
     5581 tgatttggca attgcatagt gatataaaaa ataatcagtt gataaaaaag aataccataa
     5641 ttcgtctcac caaagattcc aaattcggta aaactattta tcagaatagg aataaacatc
     5701 ccgagtatca tcaatttctt atcattcacc ttttcagtaa cagtagcttt tagcgtg
//
LOCUS       DAOOQW010000154         5712 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_17054_length_5712_cov_4.548170, whole genome shotgun sequence.
ACCESSION   DAOOQW010000154 DAOOQW010000000
VERSION     DAOOQW010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5712
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17054_length_5712_cov_4.548170"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>368
                     /locus_tag="PK281_08620"
     CDS             <1..>368
                     /locus_tag="PK281_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49450.1"
                     /translation="LGLLRMPASFDGSEYEYDNGYTRNYYGLYDNPLYTAYKNPNNSN
                     VNRVFGNTYINADPTKWLNIMYKVGIDQYSEQRRQVYHRSSAGDDNSLRYGQVNMDNI
                     NSFQLYSDLLLTAKKDFGKD"
     assembly_gap    369..378
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <379..1986
                     /locus_tag="PK281_08625"
     CDS             <379..1986
                     /locus_tag="PK281_08625"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOY49451.1"
                     /translation="IAAMKLNAIAGRGSKKDFGKDIHSSLTLGNNVWNTNFESTFSRG
                     RDLIIDDFYNLGNSIERYTSNSQERIRTAAVFFNGEIDWKSAIYLTLTGRNEWSSTFN
                     KDKNNFFYPSANISIVVSELAKLPDWFSFLKVRGALAQSGISPQPYKNRTYYSQPFFA
                     DGFTNGNSFPYLGQVGYGINSIKGNSNLLPERVNGKEVGTEMKFFQGRLGVDLTYYLQ
                     TTVDILLDKPVAPSSGFDREYVNSGKMQNQGWELALTGTPVKTTNFTWDISATWFRNR
                     SKVLELAPGVEEVDIEEAFEDIGSFAVVGQPYGVFYGSAWQRTSDGQLIIDPSTGLPV
                     IDPKRKALGSPYPDWLSGVRNTFTIGKNLSISMLWDFRKGGKIWNGTWARLNRLGQTS
                     ESAEDRDNPKVIEGVLATGFDDQGYAIAGTDKNTTEVSAFDYYRSYVGDAGGAIESQI
                     QDGSWTRLRELSASYRINFKPNAAGKKFIQYIDINATAINLLLFTKYKGVDPETSLTG
                     AGSNIQGFDYFNNPGVKTFSFGIRAGF"
     gene            2008..3399
                     /locus_tag="PK281_08630"
     CDS             2008..3399
                     /locus_tag="PK281_08630"
                     /inference="COORDINATES: protein motif:HMM:NF024181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HOY49452.1"
                     /translation="MKRIFKTMLLLSLTAAVLPGCSKYLEDATENPNDPSIGSPGSML
                     ANIEVATFSNYAGNSARRAAILTQQMSGTDGQMIQLANYQILEGDVTNEWKGIYNNAV
                     INCNLLVETYGEKNPYYAGIAKVLKALNIGLATDMWGDVPFSEAGLGLTGNTEPKYDT
                     QQSIYTGLQALLTEAIGDLSKDPSTNAYFPSGDLVFAGDAASWISSAWLLKARYANRL
                     SQVDPSGSATLALEYLNNAALTGSENDLNAVFGEGGTELNQWYAFESARGGYMRMGKY
                     FVDTLTSSADPRLPFYAALDDNGGYSGSSVDPSEGDVTTSAIGPYFGSPASVAPIATF
                     VEAKFIEAEAKLRLGDNDGASTAHNDAVKASILQITGANDDTYEAAYAAEFAGTISLD
                     KIMYQKYVALFTQVEVWNDWRRTNIPQLIANPSGVVNGIPTILPTSQDERLYNNNATV
                     ITNILSKVWWDAN"
     gene            complement(3465..5648)
                     /locus_tag="PK281_08635"
     CDS             complement(3465..5648)
                     /locus_tag="PK281_08635"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HOY49453.1"
                     /translation="MTAVEYQSFFQSYCHLYKDQQLAVLFVGRNGEDLWLNDRLIHQL
                     DKLGIQEPIKFIDTFFKEQEERIFRDLFKARIEKTTLICHYQIRTVQCRLLISALNLG
                     KNKFVGYQIEVQELQEISDLPVHELIQFPFSNPNPVVRLSKAGYLKYANPSAQEVFCT
                     DNTKLIPEIQQQFKEAIALFDSENSVVSTRIYTSDKTFLSSISLDTGGINVYAVDITE
                     SVNQEKEQSSNIAFLETVTNNSSNAVILLDSTKRIVYFNQKAQDDSGVFLNHRLVKGS
                     ALPDFKDQQFTIKLHRAIDAVFKRALKFTFEISTQIEIDTENWFRFLVSPVFDSDGAI
                     SGVYLNSINITQSRLYEAELQRTKNFYETILNQIPADIAVFDLDHSYMFLNPKAVANE
                     EIRKWMIGKNDYDYFQMKGLGTEIADKRRSIFNEVVNSRKTHDTIDKHTRADGESNYV
                     LRRFYPYVDDDELKLVIGYGIDITPIKLAEQDTLAALDKERELNQLKSHFVQMASHEF
                     RTPLATIQSSMDILNLRLSRSEFLPEDIRHTFKMHHNRIEQEISWMTEIMNNILILGK
                     LDAGRMNLRPELISIEQLISDEIQEKETISGLKNMVKISVSGNSRVMLLDSRLMRHVI
                     GNLISNALKYSEGMPAPEVYIDYQESNVNIRVRDFGIGIPEEEIPYLFTSFYRATNTE
                     NIKGTGIGLVIIKQLVEMHAGKVSLTSVINEGTEFTVELPYLAND"
BASE COUNT         1745 a         1062 c         1115 g         1780 t
ORIGIN      
        1 gttaggactt cttcgtatgc cagctagttt tgatggaagc gaatatgaat acgacaatgg
       61 ctacacgcgc aactattatg gtttgtacga caatccactt tacacagctt acaagaatcc
      121 aaataatagt aacgtaaatc gtgtttttgg taatacctac atcaatgctg atccaactaa
      181 atggcttaac attatgtaca aagtaggtat tgaccaatac agcgaacaac gtcgtcaggt
      241 ttaccaccgt tcatctgcag gtgatgataa ttcacttcgt tatggacaag taaacatgga
      301 caacatcaac agtttccaat tgtattcgga tctattgtta acggctaaaa aggattttgg
      361 taaagacann nnnnnnnnat tgctgccatg aaattaaatg ctattgcagg cagaggaagt
      421 aaaaaagatt ttggtaaaga cattcactcg tctctaacat taggaaataa cgtgtggaat
      481 accaatttcg aaagtacatt cagccgtggt cgtgatttga ttattgatga tttctacaac
      541 cttggaaatt caatcgaacg ttacacttca aatagtcagg aacgcattcg tacagcagct
      601 gttttcttca atggagaaat cgactggaaa agtgctattt atttaacgtt gacaggtcgt
      661 aatgaatggt catcaacatt taacaaggac aagaataact tcttctaccc ttctgcaaac
      721 atttcgattg tagtaagtga attagctaaa cttccagatt ggtttagttt cttgaaagta
      781 cgtggagcat tggctcagag cggtatttct cctcaaccgt ataaaaaccg tacgtattat
      841 tctcagccat tttttgctga tggatttaca aatggtaaca gtttccctta cctcggtcag
      901 gttggttacg gaatcaacag catcaaagga aattctaatc tattaccaga gcgtgtaaat
      961 ggaaaagaag ttggaactga aatgaagttc ttccaaggtc gcttaggagt tgatttgact
     1021 tactatttgc aaacaactgt tgacattcta ttagataaac cagttgcacc ttcttcagga
     1081 tttgaccgtg aatatgtgaa ctctggaaaa atgcaaaacc aaggttggga attagcttta
     1141 acaggaacac cggttaaaac aactaatttc acatgggaca tcagtgcaac ctggtttcgc
     1201 aaccgctcta aagttcttga attagctcca ggagttgaag aagtggatat cgaagaagca
     1261 ttcgaagaca ttggaagctt tgctgttgtt ggtcaacctt acggtgtatt ctacggttcg
     1321 gcttggcaac gtacaagtga tggacaattg attatcgatc caagtacagg cttaccagtt
     1381 atcgatccta aacgtaaagc cttaggaagt ccttacccag attggctttc aggcgttcgt
     1441 aacactttca ctattggtaa aaacctaagt atttcgatgc tttgggattt ccgtaaagga
     1501 ggtaaaattt ggaatggtac ctgggctcgt ttgaatcgtt taggacaaac atccgaatcg
     1561 gctgaagatc gcgacaatcc aaaagttatt gaaggtgttt tagcaacagg atttgatgat
     1621 caaggatatg ctattgcagg tactgataaa aacacaactg aagtatcagc attcgactat
     1681 tatcgttcgt atgtaggtga tgcaggtgga gctatcgaaa gtcagattca agatggtagc
     1741 tggactcgtt tacgtgaatt aagtgctagt tatcgcatta atttcaaacc caatgcagct
     1801 ggtaaaaaat tcatccaata catcgacatc aatgcaacag ctatcaattt gttgctattc
     1861 accaaataca aaggtgttga tccagaaacg agtttgacag gtgctggttc aaacatccag
     1921 ggcttcgatt acttcaataa tccgggagtt aaaacatttt cgttcggtat tcgtgccggc
     1981 ttttgatcat cctaaaaaag aaaagacatg aaaagaattt ttaaaaccat gttgttgctg
     2041 tctttaacag cagctgttct tccaggatgt agcaaatact tggaagacgc aaccgaaaac
     2101 ccgaatgatc catcaattgg ttcaccggga tcgatgttgg caaatattga agttgccaca
     2161 ttctcgaatt atgcaggtaa ttctgcacgt cgcgctgcaa ttttaacaca gcaaatgtcg
     2221 ggaactgacg gacaaatgat tcagcttgct aattaccaaa tactcgaagg tgatgtaacc
     2281 aatgagtgga aaggtattta caacaatgct gtgatcaact gcaatttatt agttgaaacg
     2341 tatggggaga aaaaccctta ctatgctggt attgctaaag tcctgaaagc tcttaatatt
     2401 ggtttagcga ctgatatgtg gggtgatgtt ccattttcag aagcaggttt aggattaaca
     2461 ggtaatactg agcctaaata cgatactcaa caaagtatct acactggttt acaagcttta
     2521 ctaaccgagg ctattggtga tttatctaaa gatccatcta ctaacgctta tttcccttca
     2581 ggagatttag tttttgctgg tgatgcggct tcttggattt catctgcttg gttattaaaa
     2641 gctcgttatg cgaatcgttt aagtcaagtg gatcctagtg gttctgccac attagctttg
     2701 gaatacttga ataatgcagc attaacagga agtgaaaatg acctgaatgc agtgtttggt
     2761 gaaggtggaa cggagcttaa tcagtggtat gcattcgaat cggcacgtgg tggatatatg
     2821 cgtatgggga aatattttgt tgataccctt acttcttctg ctgatccacg tttgccattt
     2881 tatgctgctt tagatgataa cggtggttat tccggttctt ccgtagatcc ttccgaaggc
     2941 gatgttacta cttcggctat tggtccttat ttcggaagtc cagcttctgt agcaccaata
     3001 gcaacttttg tagaagcaaa attcatcgaa gcggaagcaa aacttcgttt aggtgataat
     3061 gatggagcat ctactgctca taatgatgct gttaaagcgt cgattcttca aattacagga
     3121 gcaaacgacg atacatacga agcagcttat gcagccgaat ttgctggtac catttcactc
     3181 gataaaatca tgtatcaaaa atacgtggca ttattcaccc aagtggaagt ttggaacgat
     3241 tggagaagaa caaatattcc tcaattaata gctaatccta gcggtgttgt gaatggaatt
     3301 ccaaccattt tacccacttc tcaagacgaa cgtttgtata ataacaatgc aactgttatt
     3361 acaaatattc tctccaaagt ttggtgggat gctaattaat catcaataaa aaagaaaggc
     3421 agtcgaatta acggctgcct ttttttatgc ttattttctt ggttttaatc gttggcgaga
     3481 tagggtaatt caaccgtaaa ttctgtccct tcatttatca cactagtcag cgaaactttt
     3541 ccagcatgca tttccacgag ttgttttatg atgactaagc caatacctgt tcctttaatg
     3601 ttttcggtat ttgtagcacg gtaaaaggaa gtaaataaat acggaatttc ttcttctgga
     3661 attccaattc caaagtcacg aacacgaatg tttacgttac tttcttgata gtcgatatac
     3721 acttccggag ccggcattcc ttcggaatat ttaagtgcat tagaaattaa attcccaatt
     3781 acatgtcgca ttaaacgtga atcaagcaac atcacgcgag aattaccgct aacgctgatt
     3841 ttcaccatat tcttcaatcc agaaatggtt tctttttctt gaatctcatc cgaaattaat
     3901 tgttcaatcg aaattaattc gggacgtaaa ttcattcttc ccgcatcgag cttacctaaa
     3961 atcaaaatat tattcatgat ttcggtcatc cagcttatct cctgctcgat tcgattatga
     4021 tgcattttaa aggtatggcg gatatcttca ggtagaaatt ctgaacgact caaccttaaa
     4081 tttagaatgt ccatactcga ttgaatagta gcaagtggag ttcggaattc atgcgaagcc
     4141 atttgaacaa aatgcgactt aagctgattt aattcccgtt ctttatctaa agccgcaagt
     4201 gtgtcctgtt cagctagttt gatgggtgta atatcgatgc cgtaaccaat gaccaatttg
     4261 agttcatcat catctacata agggtaaaaa cgacgtaata cataattgga ttcaccatcg
     4321 gcacgcgtgt gtttatcaat cgtatcgtgg gttttacgag agtttacgac ttcattaaaa
     4381 atggatctac gtttatcggc tatttccgtt ccgagacctt tcatctggaa ataatcgtaa
     4441 tcgtttttcc caatcatcca tttccgaatt tcctcgttag caaccgcttt gggatttaga
     4501 aacatgtagg aatgatctaa atcgaatacg gcaatatcgg cgggtatttg attcaatatt
     4561 gtttcgtaaa aattcttcgt ccgttgcaat tctgcctcgt acaatcggct ttgggtaata
     4621 tttatactat tgaggtatac acccgaaatt gctccatcag aatcgaaaac cggacttact
     4681 aaaaaacgaa accaattttc tgtatcaatt tctatttgag ttgaaatttc aaacgtgaat
     4741 ttgagagcac gtttaaaaac cgcatcaatt gctcgatgta atttaatcgt gaattgctga
     4801 tctttaaaat cgggtaatgc acttcctttt accaaacgat gattaaggaa aacacccgaa
     4861 tcgtcttgag ccttttgatt aaaataaacg atgcgtttgg ttgaatcaag tagtatgact
     4921 gcattgctcg aattattcgt aactgtttcg aggaaagcga tattactcga ttgctccttt
     4981 tcttgattaa ccgattcggt aatatctacc gcatacacat ttattccgcc agtatctaaa
     5041 gaaatgcttg ataaaaatgt tttgtctgaa gtgtaaatac gagtagaaac aactgaattc
     5101 tcggaatcga ataaggctat tgcctcttta aattgttgtt gaatttcggg tattaacttt
     5161 gtattatccg tgcaaaatac ttcttgtgca cttggattgg catattttaa atatccagct
     5221 ttgctaagtc taacaaccgg attgggatta ctaaacggaa actgaattaa ttcatgaaca
     5281 ggcaaatcgg aaatttcttg taattcctga acttcaatct gatagccaac aaacttgttt
     5341 tttccgagat ttaatgcaga aatcaacaag cgacattgaa ctgttcgaat ttgataatga
     5401 cagattagcg ttgttttctc tattcgagct ttaaacaaat cgcgaaagat gcgttcttcc
     5461 tgctctttga aaaatgtatc gatgaactta attggttctt gaattcctag tttgtctaat
     5521 tgatggatta aacgatcatt caaccataaa tcctctccgt ttcttccaac aaatagcaca
     5581 gccaattgct gatctttgta gagatggcaa taactttgaa aaaaagattg atattcgact
     5641 gctgtcataa atcaaaaccg aggctccgaa cacatacggg aaattgctgc gaaggttctt
     5701 atttatcagc aa
//
LOCUS       DAOOQW010000155         5655 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_17265_length_5655_cov_4.806429, whole genome shotgun sequence.
ACCESSION   DAOOQW010000155 DAOOQW010000000
VERSION     DAOOQW010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5655
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17265_length_5655_cov_4.806429"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="PK281_08640"
     CDS             <1..234
                     /locus_tag="PK281_08640"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992330.1"
                     /note="catalyzes the formation of
                     (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-
                     carboxamido)succinate from
                     5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate and
                     L-aspartate in purine biosynthesis; SAICAR synthase;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HOY49454.1"
                     /translation="QKQLSKEFVRQWLIANGFQGKEGQQIPVMQEEFIEEISARYIEL
                     YEHVTGKQFVPQVYDETEVRVFANVQRFLAQLA"
     gene            complement(222..818)
                     /locus_tag="PK281_08645"
     CDS             complement(222..818)
                     /locus_tag="PK281_08645"
                     /inference="COORDINATES: protein motif:HMM:NF017816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF922 domain-containing protein"
                     /protein_id="HOY49455.1"
                     /translation="MNLSFLFPIVMLAAVPFAQQPSPYILYPTTFTKAQNDTLYWREG
                     VTLTWDDFKSKAPNQTGFAAYTYTIITMGYELKTSGETYKPKFTVHSAFQRSKSWVDR
                     KDPKAKTPEILGHEQVHFTIAEITARKLRRNLKSATYTKNYRKEIQEIYDQTLALGEK
                     MQQRYDRETRHGIDKEAQKDWSNLVLADLNYLVMYQAN"
     gene            1233..2198
                     /gene="rfaD"
                     /locus_tag="PK281_08650"
     CDS             1233..2198
                     /gene="rfaD"
                     /locus_tag="PK281_08650"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HOY49456.1"
                     /translation="MIVITGAAGFIASCLVTYLNENRYFDLVLVDDFSKKEKAPNLAG
                     KTFKEKVDRSDFIAWLDANHQMVEFVFHLGARTDTTEFDTRIFDELNLHYSQAVWNKC
                     VEYGLPLVYASSAATYGLGELGYDDNHEVVEKLKPLNPYGDSKNDFDKWALKQARAPY
                     FWAGLKFFNVYGPNETHKKRMASVIFHSFNQINEKGSVNLFRSHRPDYTDGGQKRDFV
                     YVKDLVKICYFLMLNRKHSGLYNLGTGEARTFFDLASATFAAMGKKADIHFIDTPLDI
                     RDKYQYFTEANMQKLRSIGYSDAFFSLEDGVKDYVQEYLMKGKSY"
     gene            complement(2199..3005)
                     /locus_tag="PK281_08655"
     CDS             complement(2199..3005)
                     /locus_tag="PK281_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002978935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOY49457.1"
                     /translation="MTTEKYALITGASSGLGKAFARELALRNTNLILVSLPGEGLAEF
                     AQELRDTCVEVVYYEIDLAQTNQIEWLAEDVNSKYNLFLLINNAGRGGTNRIMDVKTD
                     YINSIIQLNVTATALLTHALLPNLLNQNQAYVLNVSSMAAFSPMGFKTVYPASKSFVH
                     YFSRGLYQELRYSPVFVSVLTPGPMKTNADVSQRIERQGIFGRIGLISPERVAKKAID
                     QLYKRDTLILLGFWNGLNWLLMKTIPIGLRLPLLTRLVKREIRATEQATC"
     gene            3142..4749
                     /locus_tag="PK281_08660"
     CDS             3142..4749
                     /locus_tag="PK281_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49458.1"
                     /translation="MKKQLLACLVLLVAFTGILSAQEITIESPGARGFSGVKSINGEI
                     VFTTWFGEKTETKGMASFVLKLYDKELKEIKTTEVEISKFSEMASSAFTGKYFLFIFV
                     DAMKKNRTMVTLDAAGNVIQKKVEEDVRRALLTEENFPIIHVLNDEEFILLRPMKEKK
                     FGFDIERLDKDLNSKWTKSHIPEDGIWKAQDSKVSNGKLYLLREQNPSRSSDNYVYSV
                     QCFNVEDGEQIYSTDLTNGEDGGAPAFIRVTANGDVATGGMYFKNSKYDDKNSDGLFF
                     AIINAEGNVNQFTKKSWDSMKDQISGEFSSALLGGKTKIMVEDVIKKTDGTYMVICEQ
                     FKKANNSSMTGSGAGALLGGGSSSSSTATGPEIGFTVLDFVFFNFDASGKLSGIEVVA
                     KQNKEAKVSGKVASEKGLAIAQYMYGRGFFCYKEMIENNGKQIIVYRNDDGFKSKAYF
                     LPVGAKSTEGIPSIDMDRWVSESLNKLGKFSKATGGGQYSFNSDSNSSDTYKLYKNIT
                     SFKPGYMLLYDYNQKGLKVWLEPVPAN"
     gene            complement(4972..>5655)
                     /locus_tag="PK281_08665"
     CDS             complement(4972..>5655)
                     /locus_tag="PK281_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49459.1"
                     /translation="YLGFGSFGFRNADTLFQYTNYVTFQLRGKKGLLTSYLLSPFYAD
                     SLLQMKTDGFDESSYSFLFGQKSKSGNMLFGWMTAEGKEVIPAMYDSIGFKSSYMSPE
                     YNYAGDKWIVKKKGKCGMIKPDGTIILDAKYDAIDYFDLSKVTPVFRRLKKDGKYGLY
                     YLENDGVFFGTNWILIEPFTPYKITALRFVPRGKEKNRNYFTLFRLEDDAGKFIGYRS
                     KSGIDFFAD"
BASE COUNT         1720 a         1089 c         1140 g         1706 t
ORIGIN      
        1 caaaagcaac taagcaaaga atttgttcga caatggttaa ttgcaaacgg tttccaagga
       61 aaagaaggtc agcaaattcc tgttatgcaa gaagaattta tagaagaaat ttcggctcgc
      121 tacattgagc tttatgagca tgtaacgggt aagcaatttg tccctcaagt ttacgacgaa
      181 accgaagtac gcgtatttgc taatgtgcag cgttttttag ctcagttagc ctgatacata
      241 actaggtaat ttaaatcggc aagtaccaga tttgaccaat ctttctgggc ttctttgtcg
      301 ataccgtgtc gcgtttcccg atcgtagcgt tgttgcattt tctcgcctaa ggcaagcgtt
      361 tgatcgtaaa tttcttggat ttcctttcgg taattcttgg tgtatgtggc cgattttagg
      421 ttgcgccgta gtttacgtgc agtaatttcg gcaatggtaa aatgtacctg ttcgtgcccc
      481 aaaatctcgg gtgtttttgc tttgggatct tttcgatcta cccaggattt gcttcgttgg
      541 aaagcactgt gaaccgtgaa tttaggctta tacgtttcgc ccgatgtttt tagctcatat
      601 cccatcgtaa tgattgtgta tgtataagca gcaaatcccg tttgattagg tgctttcgat
      661 ttgaaatcgt cccaagttag cgttacacct tctcgccagt aaagcgtatc gttttgtgct
      721 ttagtaaaag ttgtcggata taaaatatac ggactcggtt gctgagcaaa gggtacagca
      781 gctaacatca caatgggaaa aaggaatgat aagttcatga gctaaaataa cggaaaaaat
      841 gagttattaa tgtgttggtt tgctcccttc gacaagctca gggagcgttt cgttgagcta
      901 gtctatgtag gtttgttatg ctgatgccgc gcgctgagcc tgtcgaagcg tgctggttcc
      961 ttaaaggacg gattgaaatc cggggttgct atagacattg gttagtattt ttctaaggta
     1021 tcccaggatt tcaatccggg gtcatcttag cactttaatt tgaaaagcac ctccattccc
     1081 gcatgagcga ttgagacgga agaccgctta gttcgccgta gctttagcga aggcgaacag
     1141 taggacttat agctcaccaa gtggcaagca aaagcgcagc ccgaaaggac atgcccaaat
     1201 tcaaaatact atatttgcag caaaattccc ttatgatcgt tattactgga gcagccggat
     1261 ttattgcatc ctgcttggtt acttacctca acgaaaaccg ctattttgat ttagtgctcg
     1321 tggatgattt cagtaaaaag gaaaaagcgc ccaatttagc tggtaaaacg tttaaggaga
     1381 aagtcgaccg aagtgatttt attgcttggc tcgatgcgaa tcaccagatg gtagaatttg
     1441 tctttcacct aggcgcacga accgatacaa ccgaattcga cacccgaatt tttgacgaat
     1501 taaacctgca ttattcgcaa gctgtttgga ataaatgtgt tgaatacgga cttcccttag
     1561 tttacgcttc ttcggcagca acgtatggtt tgggagaatt gggatacgac gataatcatg
     1621 aagttgttga aaaattgaag ccgttgaatc cttacggaga ttcgaaaaac gatttcgata
     1681 aatgggcatt gaaacaagct cgagcgcctt atttttgggc aggtttgaag tttttcaatg
     1741 tttacggacc gaatgagacg cataaaaaac gcatggcttc ggtgattttt cattcgttta
     1801 accagattaa cgaaaaaggt agtgtaaatc tgtttcgctc acatcgtccc gattacacgg
     1861 atggcgggca aaagcgagat ttcgtttacg taaaagattt ggtaaaaatt tgctatttct
     1921 tgatgttaaa tcgcaaacat tccggtttat acaatttagg aacgggagag gcgcgtacat
     1981 ttttcgattt agcatcggca acctttgctg ccatgggaaa gaaagccgac atccatttca
     2041 tcgacacgcc tttggacatt cgtgataaat accaatattt taccgaagct aacatgcaaa
     2101 aacttcgcag cattggctac tctgacgcat tcttcagcct cgaagatggt gtgaaagatt
     2161 acgtgcagga atatttgatg aagggaaaat cgtattaatc aacaagtagc ttgttcggta
     2221 gctcgtattt cgcgttttac aagccgagtt aataggggca atcgcaatcc tattgggatg
     2281 gttttcatga gtaaccaatt taaaccattc caaaatccga gtaatataag tgtatcacgc
     2341 ttgtacaact gatcaatagc cttttttgct actcgttcgg gacttatcaa accgattctt
     2401 ccaaagatgc cttgtcgttc gatgcgttga gaaacatctg cattggtttt catcggacca
     2461 ggagttaaca cgctaacaaa cacggggctg tagcgcaatt cctgatacaa cccgcgggag
     2521 aaataatgca caaatgactt actggctgga taaaccgttt tgaatcccat aggactaaat
     2581 gcagccatac tcgacacatt caagacataa gcttggttct gattgagtaa attaggtagt
     2641 aatgcatggg tgagtaaagc cgttgcagtt acattgagtt gaataatact attgatataa
     2701 tcggttttta catccattat gcgatttgtt ccgcctcgac cagcattgtt aatgagtaag
     2761 aacaggttgt atttcgaatt gacatcttct gctagccatt caatctgatt ggtttgagct
     2821 aaatcaattt cgtaataaac tacttcaaca caagtatcac gaagctcttg ggcaaattcc
     2881 gccaagcctt cacccggtag agaaacgaga ataagatttg tgtttcgaag tgccagttct
     2941 cgagcaaatg ccttccctaa accactactc gctcctgtta taagcgcata tttttctgtc
     3001 gtcatgttga acctttcctc tatgacaaca tagttgagct gttcccctat gtggatttga
     3061 aatatttttg aaatgttaaa aatgcacgct ttttttaccc ccaaagtatt atctcttacc
     3121 ttcaccccaa ataaatcata catgaaaaaa caactccttg cctgtttagt acttctggtt
     3181 gcttttacag gaattctctc tgctcaagag atcacaattg aatcacccgg cgcacgcggt
     3241 ttttcgggtg ttaaatcaat caatggcgaa atcgtattca caacttggtt tggcgaaaaa
     3301 accgaaacaa aaggtatggc tagcttcgtg cttaagcttt acgacaaaga attaaaagaa
     3361 atcaaaacca ccgaagtaga aatcagtaag ttttctgaaa tggcttcttc ggctttcact
     3421 ggaaaatatt tcttgttcat attcgtagat gcaatgaaaa agaatcgcac catggttact
     3481 ttggatgctg ccggaaatgt gattcagaaa aaagtggaag aagatgttcg tcgcgcttta
     3541 ttgaccgaag aaaacttccc aattatccac gttttgaatg atgaagaatt cattctactt
     3601 cgtccgatga aagagaaaaa atttggtttt gatatcgaac gtttagacaa agatttgaac
     3661 tctaaatgga ccaaatcaca cattcctgaa gatggtattt ggaaagctca ggattcgaaa
     3721 gtgagcaacg gtaaattgta tttactacgt gaacaaaatc caagtcgttc ttctgataat
     3781 tacgtttatt cggtacagtg ttttaatgta gaagatggtg agcaaattta ctcaaccgac
     3841 ttaacgaatg gtgaagatgg tggagctcca gcatttatcc gtgttacagc caatggcgat
     3901 gtagcaacgg gtggtatgta cttcaaaaac agcaagtacg atgataaaaa ttcggatggt
     3961 ttattcttcg ctattatcaa tgcagaaggt aacgtaaatc agttcactaa aaagagctgg
     4021 gatagcatga aagaccagat ttctggagag ttttcatctg cattgttagg tggaaaaacc
     4081 aaaatcatgg tcgaagatgt gatcaagaaa actgatggta catatatggt tatttgtgag
     4141 caattcaaaa aagcaaataa ctcaagcatg acaggctctg gtgcaggtgc attattaggt
     4201 ggtggaagtt catcttcttc tactgcaacc ggtcctgaaa tcggatttac cgttttggat
     4261 tttgtattct tcaatttcga cgcaagcggt aaattaagcg gtatcgaagt ggttgctaag
     4321 caaaacaaag aagctaaagt aagcggtaaa gttgctagcg aaaaaggtct tgccattgca
     4381 caatacatgt acggaagagg atttttctgc tacaaagaaa tgatcgaaaa taacggtaag
     4441 caaatcattg tataccgcaa cgatgatgga tttaagagca aagcttattt cctacctgtt
     4501 ggcgctaaaa gtaccgaagg cattccaagc atcgacatgg atcgttgggt gtctgaaagc
     4561 ctaaataaac taggtaaatt ttctaaagca actggcggcg gacaatactc gttcaattcc
     4621 gatagcaaca gcagcgatac ctacaaatta tataagaaca ttacctcgtt caaaccaggg
     4681 tatatgttgt tatacgatta caatcaaaaa ggattaaaag tttggttaga acctgttcca
     4741 gctaattaat ttaattcatc taaaaatccc ctgttaattc aaattagcag gggatttttt
     4801 attttatcta tttgtgcagt gtccgcgctt tcgcttgcca ctatgtgtgc tgcaagtcct
     4861 cacagtatgc cttcgccaag gctacggcat acgctgcggg cttttagctc tatcgctact
     4921 gctttttcta ttctgattta tttaggtcaa caaggaatta tttgggtcaa attaatcagc
     4981 aaaaaaatca attcctgatt tacttcggta accaataaat ttacccgcat catcttctaa
     5041 acgaaaaagc gtaaagtaat ttcgattctt ctctttccca cgcggaacaa aacgaagcgc
     5101 tgttatttta taaggtgtaa atggctcaat taaaatccaa tttgttccaa aaaacactcc
     5161 atcattttcg aggtaataca aaccatattt tccatctttt ttcaagcgtc taaaaacggg
     5221 tgttactttt gataaatcga aataatcaat ggcatcgtat ttcgcgtcta aaatgattgt
     5281 tccatcaggt ttaatcattc cacatttacc cttctttttt acaatccatt tatcacctgc
     5341 ataattatat tcaggcgaca tataagaact tttaaagcca atactatcat acatagcagg
     5401 aattacttct ttcccttctg ccgtcatcca tccaaacaac atattacccg atttactttt
     5461 ttgtccaaat aaaaacgaat agctcgattc atcaaaacca tctgttttca tttgtaataa
     5521 agaatccgca taaaaaggag ataataaata actagttaaa agcccttttt tacctctcaa
     5581 ttgaaacgtt acataattag tgtattgaaa taatgtatca gcatttctaa aaccaaaaga
     5641 tccaaaacct aagta
//
LOCUS       DAOOQW010000156         5501 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_17867_length_5501_cov_4.156812, whole genome shotgun sequence.
ACCESSION   DAOOQW010000156 DAOOQW010000000
VERSION     DAOOQW010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5501
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17867_length_5501_cov_4.156812"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(185..1429)
                     /locus_tag="PK281_08670"
     CDS             complement(185..1429)
                     /locus_tag="PK281_08670"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex dihydrolipoamide
                     acetyltransferase"
                     /protein_id="HOY49460.1"
                     /translation="MAEIVKMPKLSDTMEEGVVSKWHKKVGDKVKSGDLLAEIETDKA
                     TMDFESFQDGVVLYIGVEEGKGAPVDSILAILGKEGEDYKSLISGASGGSVTKTESAP
                     AEAAAPIASSVPAAVSTPVVSASADDSSRMKASPLAKKIASDKGIDINQVQGSGDGGR
                     IVKRDVENFSPSVSSAKPAAAPYVSVGKESYSEENVSQMRKVIARRLAESKFSAPHFY
                     LTIEIDMDNAIAARSAINATGDVKISFNDFVVKASSMALRKHQTINSSWLGDKIRRNH
                     HVHIGVAMAVEDGLLVPVVRFADNKSLSQISAEVKDYSAKAKAKKLQPQDWEGNTFTI
                     SNLGMFDIEEFTAIINPPDACILAVGSIKQKPVVKNGAVVPGNVMKLTLSCDHRVVDG
                     ASGAAFLKTLKEYLEQPVMMLV"
     gene            complement(1449..2444)
                     /gene="pdhA"
                     /locus_tag="PK281_08675"
     CDS             complement(1449..2444)
                     /gene="pdhA"
                     /locus_tag="PK281_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HOY49461.1"
                     /translation="MAKPKFKKEQYLKWYEEMLLWRRFEEKSSQLYIQQKIRGFCHLY
                     IGQEAVLAGAMSATRLSDNMITAYRDHVQPIAKGVSPNAVMAELMAKATGCSKGKGGS
                     MHMFSAEHKFFGGHGIVGGQIPLGAGIAFADKYNGNDNVTLTYMGDGAVRQGALHEAF
                     NMAMLWKLPVIFIIENNNYAMGTSVQRTTNVHDLYKIGLSYDMPSKAVDGMSVEDVHD
                     AMVWAVDRARKGEGPSLLEMKTYRFRGHSMSDPGKYRTKEEVESYKSQDPIEQVLETL
                     RKNKWITEAEIEATEDRIKAVVEESVVFAEESPYPTVDELYKDVYVQDNYPYILD"
     gene            complement(2518..3009)
                     /gene="cdd"
                     /locus_tag="PK281_08680"
     CDS             complement(2518..3009)
                     /gene="cdd"
                     /locus_tag="PK281_08680"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006260273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HOY49462.1"
                     /translation="MRELHFTSLVREYDQLENLPETDQKLMNAAIEASSRAYAPYSHF
                     YVGAAVLLDDGTIVLGNNQENVAYPSGLCAERTALFAAGANFPGKGVLKVAIAAHSDD
                     FTVETPVYPCGACRQVMSEYEQLSKRPMEILMMGAQGKVHVVNGLINILPVAFNADNL
                     KRK"
     gene            complement(3046..3522)
                     /gene="ispF"
                     /locus_tag="PK281_08685"
     CDS             complement(3046..3522)
                     /gene="ispF"
                     /locus_tag="PK281_08685"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HOY49463.1"
                     /translation="MNIRVGQGVDVHQLAEGYDLWLGGLKIPHHKGAVGHSDADVLIH
                     AICDALLGAANLRDIGYHFPDTSSEFKGIDSKILLRRVTDLLFEKGWKVGNVDTTLIL
                     EQPKIKPYIEQMQETLAPLMGIETDAVSIKATTNETMGYVGRQEGVLAFATALIYR"
     gene            complement(3522..4769)
                     /gene="porV"
                     /locus_tag="PK281_08690"
     CDS             complement(3522..4769)
                     /gene="porV"
                     /locus_tag="PK281_08690"
                     /inference="COORDINATES: protein motif:HMM:NF033710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system outer membrane channel
                     protein PorV"
                     /protein_id="HOY49464.1"
                     /translation="MTKHTFYTLAFTVAGCFAAQQADAQISSSQLNGQTINTITTAVP
                     LLMISPDARAGALGDAGGAMGPDANSIHWGSAKLASIEGSSGISLSYIPWLRNLVPDI
                     NLGYLSWYKKMKKRQTIGGSLRYFSLGNIVFTDIVGNTIGQFNPNEFAIDFAYARKFS
                     DRWSGGITMRYIYSNLTNGIDVQGTPSFAGQTVAADISTYYSNPDLKLGGKKATYSFA
                     MAITNIGGKISYTKTADRNFIPINLRIGNSLKLKLDDYNSFTFLFDLNKLMVPTPPVR
                     SDGSALYPGIPQGEVAFGKENNVPIVTGMLQSFSDAPGVLQGDGSRKVLSEELREINP
                     CGGVEYSYNNVFAIRTGYFYEHPTKGNRRYFTLGAGVKFNVFTLDLAYLIPTQQRNPL
                     ENTLRFTLQFDFGAFQSQNKSSE"
     gene            complement(4829..>5501)
                     /locus_tag="PK281_08695"
     CDS             complement(4829..>5501)
                     /locus_tag="PK281_08695"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY49465.1"
                     /translation="NPTTDNTGPEIKLFMNNEQFVSGGLTDQNPDLLAYVQDDIGINT
                     VGTGIGHDITAILDGDASNPFVLNDYYEAEQDNFRKGKIRYPFKNLAPGKHTITLKVW
                     DVANNSSTASIEFIVVKSEELSLEHVLNYPNPFTTHTQFYYEYNQPGVPVQVDIQVFT
                     VAGKMVKSINTSQISNGYRSEAITWDGLDDFGDKIGRGVYVYRLRVRTPDGKSAEKIE
                     KLVIL"
BASE COUNT         1648 a         1220 c          947 g         1686 t
ORIGIN      
        1 cttcgggtcg cgtttttgct cgccacaatg tgggctaata gtcctcttgt tcgccttcgc
       61 ttccgctgcg gcgcacactg cgggctactg cctcaaccgc tcatacaaag tccatacacc
      121 aactaacata aaaaaagccc tgtaacatca ttgtcacagg gctttttctt gtttaattga
      181 aaccctatac aagcatcatt actggttgct cgaggtattc ttttagtgtt ttcaggaaag
      241 ctgcaccact ggctccatca acaacacgat gatcgcagct gagcgttagt ttcatcacat
      301 ttccaggaac tacagctcca ttttttacca ctggtttctg tttaatcgaa cctacagcca
      361 aaatacacgc atctggagga ttgataatag ctgtaaattc ttctatatca aacattccaa
      421 gattactgat agtgaaagta tttccttccc aatcttgtgg ttgtagtttt ttcgcttttg
      481 ctttagctga gtaatctttt acctctgccg aaatctgcga taagctttta ttatctgcaa
      541 atcgaactac tggtacaagc aatccatctt caacagccat ggcaacacca atatgtacat
      601 ggtgattacg acgaatttta tcgcctaacc aagatgaatt aattgtttga tgtttacgaa
      661 gcgccataga ggaagctttc accacaaaat cgttgaacga aatcttcaca tcaccagttg
      721 catttatagc cgaacgagct gctattgcat tgtccatgtc gatttctata gttaagtaga
      781 aatgcggagc actaaactta ctttcagcca aacggcgcgc aattacttta cgcatttgag
      841 atacattctc ttccgaataa ctttcttttc ctacagatac ataaggagcc gcagctggtt
      901 ttgcagatga aaccgaagga ctgaaatttt ccacatcgcg tttcacaatg cgtccaccat
      961 cacccgatcc ttgaacttga ttgatatcaa ttcctttatc tgaagcaatt ttcttagcta
     1021 atggagatgc cttcatacga gatgaatcat cagccgaagc actaacaaca ggggtagaaa
     1081 ctgctgctgg tacagaagaa gcaataggag cagccgcttc agcaggagca ctttcggttt
     1141 tagtaaccga acctcctgaa gcaccactaa tcaatgattt ataatcttct ccttctttac
     1201 ctaaaatagc tagaatcgaa tcaacaggtg cgcctttacc ttcctcaaca ccaatgtata
     1261 ataccacacc atcttgaaat gattcaaaat ccatggtggc tttatcagtt tcgatttcgg
     1321 caagcaaatc tcctgatttt actttgtcgc ccactttctt atgccatttc gaaacaacac
     1381 cttcttccat ggtatcgctc aacttgggca ttttgacaat ttctgccata tcgttctatt
     1441 ttcctgtatt aatcgagaat gtaggggtag ttatcttgaa catatacatc tttgtatagt
     1501 tcatcaaccg ttggataagg tgattcctca gcaaaaacaa ccgattcttc caccacagct
     1561 tttatacgat cttcggtagc ttcaatttcg gcttctgtaa tccatttgtt tttacgaagt
     1621 gtttctaaca cctgctcaat tgggtcttga gatttataag actcaacttc ttctttcgtt
     1681 cggtatttac caggatccga catggaatgt ccgcggaaac gatacgtttt catttccaat
     1741 aaacttggac cttcaccttt acgagcgcga tcaacagccc aaaccattgc atcatgtaca
     1801 tcttcaacgc tcatcccatc aacagctttc gaaggcatat cgtatgataa accaatcttg
     1861 tacaaatcgt gcacattagt cgtacgttgc acagaagtac ccatggcgta attgttattc
     1921 tcaatgatga aaataacggg caacttccac aacatagcca tattaaaagc ttcgtgtaat
     1981 gctccttgac gaacagcacc atcgcccata taggtcaagg ttacattatc gttcccattg
     2041 tatttatctg cgaaagcgat acctgcacca agtggaattt gacctccaac aataccatgt
     2101 cctccaaaaa acttatgttc agcagagaac atgtgcattg aaccaccttt accttttgaa
     2161 caaccagtag ctttagccat caattcggcc attacagcat taggtgatac accttttgca
     2221 attggctgaa cgtgatcgcg ataagctgtg atcatgttat cgctcaaacg tgtagcactc
     2281 attgcaccag ctaaaacagc ttcttgtcca atgtataagt gacagaatcc acgaatcttc
     2341 tgttgaatat acaactggct tgatttctcc tcaaaccggc gccacagaag catttcttca
     2401 taccatttaa ggtattgctc tttcttgaat ttaggctttg ccatttcttt ttctactctt
     2461 gggtgggcaa atttataaga atctattgtt ctgcaataat aattgcgaaa aaacaggtca
     2521 ttttcgtttt aaattatcgg cattaaaggc aacgggtaaa atatttatca atccgttaac
     2581 tacatgcacc tttccttgcg cacccatcat taggatttcc atcgggcgtt tacttaattg
     2641 ttcatactcc gacataactt gacggcatgc gccacaagga tagacaggag tttcaaccgt
     2701 aaaatcatct gaatgagctg caattgcaac ctttaacaca cctttccccg gaaagttcgc
     2761 tccagcagca aataaggcag ttcgttcggc acacaatccc gaaggatagg caacattttc
     2821 ctgattatta ccgagtacga ttgtaccatc gtcgagtaat acggcagcgc caacgtaaaa
     2881 atgtgaataa ggagcataag ctctagaact cgcttctata gcggcattca ttaatttctg
     2941 atctgtttct ggaagatttt ccagttgatc atattcacgt actaatgagg taaagtgcag
     3001 ttctcgcatc cgggtaaaga tacaaaggag cagttaacaa gcaaatcaac gataaatgag
     3061 tgcggtagca aaagccaaaa cgccttcttg ccttcctaca tatcccatag tttcgttagt
     3121 agtagctttt attgaaactg catctgtttc aatccccata agcggagcaa gtgtttcttg
     3181 catttgctca atatagggtt tgattttagg ctgctccaaa attagtgttg tgtccacatt
     3241 acctactttc caacctttct cgaaaagtag atcagtgact ctacgaagaa gaattttact
     3301 atcaatccct ttaaattcag atgaagtatc cggaaaatga tatccaatat cgcgcaggtt
     3361 agcggcaccc aaaagtgcat cacaaatcgc atgaattaac acatcagcat ccgaatgccc
     3421 aacagcacct ttgtgatgtg gaatcttcaa tcctccaagc cataaatcat atccttcagc
     3481 gagttgatgt acatcaaccc cttgtcctac tcggatattc attattccga actcttattc
     3541 tgagattgga atgcgccgaa atcgaattgc aaggtaaatc gaagtgtatt ctccaatgga
     3601 ttacgttgct gagttggaat taaatatgct aaatccaaag taaacacgtt gaatttaaca
     3661 cccgctccta aagtaaaata acgacgattt ccttttgtag gatgctcata gaaatatcca
     3721 gttcgaatag cgaacacatt attataactg tattcaacac cgccacatgg gttaatctct
     3781 cttaactctt cacttaaaac ttttcgagat ccatcaccct gtaatacacc tggagcatct
     3841 gagaaagatt gtagcattcc agttacaata ggtacattat tttcttttcc aaatgctact
     3901 tcaccttgag ggatacctgg atataaagca gaaccatctg aacgtactgg tggagttgga
     3961 accatcaatt tattcaaatc aaaaagaaac gtaaatgagt tgtaatcatc caacttcaat
     4021 ttcaatgaat ttccaattct taagttaatt ggaatgaaat ttcgatctgc cgttttagta
     4081 tatgatattt tccctccaat gttggtgata gccattgcaa acgaatacgt tgcttttttc
     4141 ccaccaagct tcaaatcagg attagaataa taagtcgaga tatcggcagc tacggtttga
     4201 cctgcaaaac ttggagttcc ttgcacatca attccattag tcaaattaga atatatgtat
     4261 cgcatcgtaa ttccaccact ccaacgatca gaaaatttac gtgcataagc gaaatcgatt
     4321 gcaaattcat tgggattaaa ctgaccaatg gtattaccta cgatatcagt aaaaacgata
     4381 ttccccaaag agaaataacg caatgaacca ccaatagttt ggcgtttctt catcttttta
     4441 taccacgaca aataaccgag attgatatcg ggtaccaagt ttcgtagcca tggaatgtaa
     4501 gaaagcgaaa tacctgaact tccttcaatg gatgctaatt ttgcagatcc ccaatgaatt
     4561 gagtttgcat ctggtcccat tgctcctccc gcatctccta aagcaccagc tcgtgcatca
     4621 ggcgatatca ttaataaagg aacagctgta gtaattgtgt tgattgtttg accattcaac
     4681 tgacttgaag aaatctgtgc atctgcttgt tgggcagcaa agcatcccgc aacagtaaag
     4741 gcaagtgtat aaaaagtgtg ttttgtcatc agctttttca aaatatgggt caaatatagt
     4801 atttcagtca ttgtagcctt gtacggtttt ataagatgac aagtttctca attttttcag
     4861 ccgattttcc atcaggtgtc ctaactcgta atcggtatac ataaacacct ctaccgatct
     4921 tatcaccaaa atcatctaat ccatcccaag taatagcttc agaacggtat ccattactta
     4981 tttgtgatgt attaatggat ttaaccattt taccagctac tgtaaaaact tggatatcaa
     5041 cttgaacagg cactcccggt tggttatact cataataaaa ttgagtatgg gtggtaaatg
     5101 gattagggta atttaataca tgttcaagag ataattcttc cgatttaaca acaatgaatt
     5161 caatagaagc agttgaagaa ttatttgcaa catcccaaac ttttaatgta atggtatgct
     5221 ttcctggggc taaattttta aatggataac ggattttacc ttttcgaaaa ttatcctgct
     5281 ctgcttcgta ataatcgttt aatacaaatg gattactagc atcaccatct aatatggcag
     5341 ttatatcatg tccgattcca gttcctacgg tattaattcc aatatcatct tgaacatagg
     5401 ccagcaaatc gggattttga tctgttaatc caccagaaac aaattgctca ttattcataa
     5461 ataatttaat ctcaggtcca gtgttatcag tagttggatt g
//
LOCUS       DAOOQW010000157         5481 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_17943_length_5481_cov_3.211205, whole genome shotgun sequence.
ACCESSION   DAOOQW010000157 DAOOQW010000000
VERSION     DAOOQW010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5481
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17943_length_5481_cov_3.211205"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            173..3004
                     /locus_tag="PK281_08700"
     CDS             173..3004
                     /locus_tag="PK281_08700"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49466.1"
                     /translation="MKTSILQLFLFIGLLALQTNLLAQNLPATQSCESNCFITEIIKA
                     EKNGSCTNYEFKVSYLTGCDHGLSHYTVDIPCGDLNNLSNSKGWKQEYGYDPTTKLKG
                     FKIDNIPDFGKTSLKSFTVKFTLCPDGGRCSNNNLCVDELKCWQPVVAYKAATCVFYD
                     TLESKLCQNLSASLQQKNISCFGAADGNLAVVVAEGKAPYTYLWSNGASTSSISNLVA
                     GNYSVVIRDASGVQKELAATISQPEEIVISGIISNVTCNGQSNGAIDVSVLGGQGNYT
                     YLWSNGATSQDLASIVQGTYSVTVKDSVGCSLQKSFSITNGSQVTMSATAVLPSCTQS
                     NGSIDITVSGGTEPYTYLWANGATTEDIQNLSAGIYKVIVKDGSGCSTEFTYSLRENN
                     TLKLTSIVKQTTCLDDGSGAIDLSVTGGTAPYTYIWTNGSISEDLTNLIAGVYRVTVT
                     DANGCSATLAISVSKKTFQVNSQVIQPSCNQGGSISITPGDGVAPYTYSWDNGETGNS
                     ITDLEAGIYKVTITDATGCTRLLTYSIENPVAITSSASVSNSQCNAEGSYNIDLSVSG
                     GNAPYTFVWSNGATTEDLNSLQSGTYTVIIADTNGCSTTKEVLVAGSAVGWSCLITQP
                     DSIAVCNSINNTLGTSITGATSYQWTVESSDNKWIITSGASSQSIGYTAGDENSNATF
                     TLTIVKDGCTQTCSYTATTCTSDDNGNGDGGDDGDGDGTDNEMCDECFDSSVTLISSD
                     GLCKTYEVIVSTNGNCRHELSHWDIAIPCGNVTNTWNSGGWKMEIGKDPTTGLNGLKV
                     DDIDGFGKEPATLKVTFTICGDYACKETLKDWAPVVAYKAGQCIAIDTLGHDDDNGDD
                     GVKVCAYPNPFHGNLKFDWKCNEDDYVELEIIDKCGRQVHQVYKGNVRKGESYSFECN
                     DENLKEDMYIYRFYSGKRKTQYGKLIKKH"
     gene            complement(3818..>5481)
                     /locus_tag="PK281_08705"
     CDS             complement(3818..>5481)
                     /locus_tag="PK281_08705"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOY49467.1"
                     /translation="SNIAQKAYQKEENFSASFELQSINDIAPGRDLSNEIGTDWGYGS
                     IIIFTVLTLLILLPACFNYSNISISRALKRMKEIGLRKVMGGHRNQILGQFIIETVII
                     TFVSLSLSYYIFALVRYEFLSVIVGGSDILNLDTNARIIFYFVLFALIVGILTGIIPG
                     LYYANLNPIQALKSKPTGNNAKFSLRKTLTVFQFALSLGFIMSVVIVLNQHRQTLNYD
                     FGFNQANILDVNLQGVDPEIFKNEFSKLSSVQSISMSSSVMGAGYSGIAWVKGIDQSD
                     SVDVAQLFIDHNYLSNLNLKLLAGTNFTENTSTNSNRVIVNEEFLKHFKIKQPIDAID
                     QIFLLEDEQEVVVIGVVQNFHYTSLHDPIKSFFFRFDESQFNIANVKVASTDMFTSIG
                     EMESTWKIIGGEKKFESKFFEDEIADSYSFYFAMVKICGFLGLLAITISCLGLLGMVV
                     FTVENRTKEIGVRKVMGASTSTITMLLSKDFTKLMFIAALIATPLTYLFFDRVFLRMQ
                     AYKMPIGALEIIVSLLLLFVLGFATILSQTVKAAKANPVDTLRNE"
BASE COUNT         1716 a          987 c         1134 g         1644 t
ORIGIN      
        1 attcaaataa taccttacat cgttgcggtt ctcgaattct tgattcctgg actctgtaac
       61 cctcacttcg ccctgctgtt cgcggttttt atcgggagca taaaccattc gtatttgcaa
      121 aaggtattat aggcctgtct tctcgtatga agcattactt acaccaatgc ttatgaaaac
      181 aagtatactt caactattcc tatttatagg acttcttgcc cttcaaacta atctgctcgc
      241 gcaaaatttg cctgctaccc agtcatgcga aagcaattgc ttcatcactg aaatcataaa
      301 agctgagaaa aatggctcat gcacgaatta cgaattcaaa gtttcatatt taaccgggtg
      361 cgatcatggg ctttcacatt acacggttga cataccttgt ggagatctta ataatctctc
      421 taattcaaaa ggctggaaac aagagtatgg ctacgatcca accacaaagc ttaaaggctt
      481 taagattgat aacattccag actttggcaa aacttcttta aaaagcttta cagtaaaatt
      541 tacactttgt cctgatggag ggagatgttc taataacaac ctgtgcgttg acgaattaaa
      601 atgctggcaa cctgtagtag cttacaaagc agctacgtgt gttttttatg atacccttga
      661 aagtaaattg tgtcagaatc tttccgctag tcttcagcag aagaatattt catgtttcgg
      721 agcagcggac ggaaatctag cggttgttgt tgctgaaggt aaagcaccgt atacgtatct
      781 atggtcaaac ggtgcttcaa catcatctat ctccaatctt gttgccggaa attatagtgt
      841 tgtcattcgt gatgcatctg gcgtacagaa agaattagca gcaactattt ctcaacctga
      901 ggaaattgtt atttctggaa taatatcaaa tgttacgtgt aacgggcaat caaatggtgc
      961 aattgacgtg agtgttttag gtggtcaagg taattataca tatctatgga gtaatggtgc
     1021 tacatcacaa gaccttgctt caatagtaca gggaacatac agtgttacag tgaaggacag
     1081 tgtcggttgt tcacttcaaa aaagtttctc gataacaaat ggttcgcaag taacaatgtc
     1141 ggctactgct gttttaccat catgcacaca atcaaatggt agtattgaca tcaccgtgag
     1201 tggtggaact gaaccataca cgtatttatg ggcgaatggt gcaacaacgg aagacattca
     1261 aaatctttca gctggtattt acaaagttat tgtaaaagat ggatcaggtt gctctactga
     1321 atttacctat agtttgagag agaacaatac acttaagctt acatccattg taaagcaaac
     1381 aacatgtctc gatgatggtt ccggtgctat tgacctttcg gtaaccggag gaacagcacc
     1441 gtacacttat atttggacga atggctcgat ctctgaagat ttgacgaatc ttatagcggg
     1501 tgtttataga gtaacggtaa ccgatgccaa tggatgctct gctacactgg ccattagtgt
     1561 atctaagaaa acattccagg taaattctca ggttattcaa cccagttgca atcaaggtgg
     1621 gtcaatttca atcactccag gtgatggtgt tgcgccttat acgtactcat gggataatgg
     1681 tgaaacagga aattcaatca cagatctgga agctggtatt tataaagtca ctatcactga
     1741 tgctacgggt tgtacgcgtt tacttaccta ttcaattgag aatccagttg ctattacttc
     1801 ttccgcttcg gtatcaaaca gtcaatgcaa tgcagaaggc tcttataaca tagacttaag
     1861 tgtaagtggg ggaaatgctc cctacacatt tgtttggtcg aatggtgcaa ctacagaaga
     1921 tttgaattcg ctgcaatcgg gtacgtacac cgttattatt gcagacacga atggctgttc
     1981 aacaacaaag gaagtgttgg ttgcgggatc ggctgtcggg tggtcttgcc tcattactca
     2041 accggactct atagcagttt gtaattcaat caataacaca ttaggaactt caataacagg
     2101 agcaacaagc taccaatgga cagttgagag ttcagacaat aaatggatta tcacaagtgg
     2161 tgcatcaagc caatctattg ggtatactgc gggcgatgaa aatagcaatg ccacttttac
     2221 attaacgatt gtaaaggacg gatgtactca aacgtgttca tataccgcta ccacttgcac
     2281 tagtgatgac aatggaaatg gtgatggagg tgacgatgga gatggagatg gtactgataa
     2341 tgagatgtgc gatgagtgtt tcgattcatc ggttacattg ataagctcag atggattgtg
     2401 taaaacatat gaagtgattg tcagcacaaa tggaaactgc cgtcatgaac tttcgcattg
     2461 ggatatcgcg ataccttgcg gtaatgtaac taacacctgg aattcgggtg ggtggaaaat
     2521 ggaaataggt aaggatccaa ccactggatt aaatggactg aaggtagacg atattgatgg
     2581 ctttggaaag gaacctgcaa cattgaaagt gacgttcaca atttgtggtg attatgcatg
     2641 taaagaaaca ctgaaggatt gggctccggt tgtagcgtat aaggcgggtc aatgtattgc
     2701 aattgatacc ttaggccatg atgacgataa tggagatgat ggtgttaaag tgtgtgcata
     2761 tccgaatccg ttccatggca atttaaaatt cgattggaaa tgtaatgaag acgattatgt
     2821 agaattggaa attatcgata agtgcggaag acaagttcat caggtctata aaggaaatgt
     2881 tcggaaaggt gaatcgtatt catttgaatg taatgacgaa aaccttaaag aagatatgta
     2941 catctatcgc ttttacagtg gaaagagaaa gacgcagtat ggaaaattaa taaagaaaca
     3001 ttaagccaat cccactatgc ttgatgcagg aggccagcga gtgctggcct ctttttattt
     3061 gagaaaagct atattatttc aatattagtt tcgcacgata atatcaatat atttgaatta
     3121 tgatatttgt tgtgtatcgt ctttaccgcg gttacgatct tagcgctctc aactttttgt
     3181 atatcttatt atttgcagcc attaccttgg gttttattta cggaattgat tttctgtatc
     3241 ggaaattaaa aaagaagacc aggtaaaatt cattccgtat tctatttctt tggtttgata
     3301 tttgattacc tgtggtcgcg cattaagact aagttgcgtt gatgctcgtc aaatgttaat
     3361 cttatgttaa agaaatggat tatcggagtc gcttcgttta gcatattaag ttttttaatt
     3421 ctccgactag tgctcaatca tgttaatggt gtcaaagaag aaaggcaatg gtacatcgat
     3481 cagcttcatt ttgaattttc tgcaacgatt gattctgtcc gatcaagaca tattttgttt
     3541 catttgaatc gaacagatat cgattggaat acggagcaaa aactgaatga agtacttcaa
     3601 ttcaacggac gtctggattt attcctttat cggcccaact ataaaattga acttatgatt
     3661 ccagatgcta atagatatat gaagggtgat agcctctatt tcaattcaga taagaatgtt
     3721 attgcactat atagagacca tcatatactt gctgaatatt cggtggtaag ttccttgaaa
     3781 ggacggccgt tttgagtatt gaacgcgtta attttaacta ttcattcctc aatgtatcaa
     3841 ccggattcgc tttggccgct ttcaccgttt gggaaagaat ggtagcaaaa cctaatacaa
     3901 acagcagcaa taaacttact ataatttcca aagcgccaat gggcatctta tatgcctgca
     3961 ttcgtaagaa aaccctatca aaaaatagat aggttagtgg tgtagcaatt aatgctgcga
     4021 tgaacataag cttcgtgaaa tcctttgata acaacatggt gatagtagag gttgaagcac
     4081 ccattacttt tcgaacacca atttcttttg tgcgattctc tactgtgaag acgaccatgc
     4141 ctaataatcc taaacaagaa atagtgatgg cgagtaaccc aagaaaacca caaatcttga
     4201 ccatggcgaa gtagaacgaa taggaatctg cgatttcatc ttcaaagaat ttagactcaa
     4261 acttcttttc gccaccgatt attttccatg tggattccat ctccccaatg gaagtaaaca
     4321 tatctgtgga ggcaacttta acgttggcaa tgttaaactg actttcatca aaccgaaaga
     4381 aaaaactttt gattggatca tgcagactgg tatagtgaaa gttttggacc acaccgataa
     4441 caaccacttc ttgttcgtct tccagtaaaa agatttgatc aatggcatcg attggttgct
     4501 ttatcttgaa atgttttaag aattcttcat tgacaataac acgatttgaa ttggttgaag
     4561 tattctctgt gaaattcgtt cctgccagta atttcagatt aagattgctc aggtaattat
     4621 gatcaataaa caattgtgcc acatcaaccg aatcggattg atcgattccc tttacccatg
     4681 caatcccgga gtatccggct cccattacac tagatgacat cgatatggat tgtacagacg
     4741 aaagtttact gaattcattt ttgaatattt cagggtcaac tccctgaaga ttgacgtcaa
     4801 gaatgtttgc ctggttaaat ccaaaatcat agttgagggt ttgacgatgt tgatttaaca
     4861 caataaccac actcatgata aagccaagcg acaatgcaaa ttgaaaaaca gtaagtgttt
     4921 ttctcagact gaatttagca ttgtttcctg taggcttgct tttcagtgcc tgaatcggat
     4981 tgaggtttgc atagtataag ccaggaatta tccccgtgag tattccaaca atcaaagcaa
     5041 aaagaacaaa atagaaaata atacgggcat tggtatcaag atttagaata tcactgcccc
     5101 caacaataac cgataggaat tcgtaacgaa caagagcgaa gatgtaataa gagagactga
     5161 gagaaacaaa tgtgatgatt acggtttcta tgataaattg cccgagaatc tgatttcggt
     5221 gcccacccat tactttgcga agtccaattt ctttcattcg cttaagagcc cgcgaaatag
     5281 aaatgttaga ataattaaag caagcaggaa gtaatattaa aagggtaaga acagtaaaaa
     5341 taataattga accgtacccc caatcggttc caatttcatt ggataaatcg cgtccaggtg
     5401 caatgtcatt gatggattgc aattcgaagg atgcgctaaa gttctcttct ttttgatagg
     5461 ctttttgtgc aatgttgctt a
//
LOCUS       DAOOQW010000158         5471 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_17979_length_5471_cov_3.373523, whole genome shotgun sequence.
ACCESSION   DAOOQW010000158 DAOOQW010000000
VERSION     DAOOQW010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5471
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17979_length_5471_cov_3.373523"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(154..600)
                     /locus_tag="PK281_08710"
     CDS             complement(154..600)
                     /locus_tag="PK281_08710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49468.1"
                     /translation="MNILTKLFGSGAKEVVTSVGTVLDKLITNKEERIKAQLEVEKEV
                     NRHFEALETNSLKQIELEIQDRSSARVREVDLAKTGRWDFLMYLTGLIILSAFGAIVY
                     ASIWMKVEGEHFVRISTMVETLTVGISGYYFGSSKGSHDKNKALLK"
     gene            complement(640..1026)
                     /locus_tag="PK281_08715"
     CDS             complement(640..1026)
                     /locus_tag="PK281_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49469.1"
                     /translation="MKTGERVFTWSFDWLINRIFWPVVPVPKVCSRTHGYFPTEGIAT
                     AQSTGISTAHQSGLPQRFVARDLPDRRTSVIEFRGSKSFYELLKSAEATTSSPLFTIR
                     YCGDIGVQRHDRKSFVRNKDFETTHY"
     gene            complement(1013..1393)
                     /locus_tag="PK281_08720"
     CDS             complement(1013..1393)
                     /locus_tag="PK281_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49470.1"
                     /translation="MSEIREAFYKFVPYLKIFITLFVMVIIVIPLLSSIFSKRIRNWL
                     RAGIEGENSKLDLNEVWEVAYLYMALGSYLMLIYMIVIKTLYQVQYSWEEYMLMFFGT
                     AGSNGVAAFIIYIKQKGKDSNENG"
     gene            complement(1406..1735)
                     /locus_tag="PK281_08725"
     CDS             complement(1406..1735)
                     /locus_tag="PK281_08725"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HOY49471.1"
                     /translation="MNYIVKVGDTLSKIARDALGDITLWQDLAKHNKIANPNLIRIGQ
                     VINLDIPKRMVIDITDQNNSGIVNQLNTTSHGNGKVLDWFAGFALSVLAALTVKKILQ
                     EKGKRKN"
     gene            complement(1732..2376)
                     /locus_tag="PK281_08730"
     CDS             complement(1732..2376)
                     /locus_tag="PK281_08730"
                     /inference="COORDINATES: protein motif:HMM:NF017638.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 108 family protein"
                     /protein_id="HOY49472.1"
                     /translation="MANFDIAYKTYILPNEGGYANVAADKGGETYAGIARNYNQAWQG
                     WTFIDFEKRKLSGKPIKLNSKFPSIDYLVTDFYLNRWNSIRLTEIVNQEIANLLYDFH
                     VHSQTTAIKFIQRIVGVPADGKMGTTTIATINKGNAAKIHDALLSERKNLLESLIAKN
                     PSQEVFREGWMARLAKFPTLLSPTTGGAALGLLAAGAIVLYVISRSEDFKKLAT"
     gene            complement(2381..3049)
                     /locus_tag="PK281_08735"
     CDS             complement(2381..3049)
                     /locus_tag="PK281_08735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49473.1"
                     /translation="MEIILNHALKRKQVSSLGVTIVPVVYDPKTYKFAQPNTSLAHKG
                     EVVWGCADWMIFHKRLVEGFIAGRFASKKKYTKEQAIELSNQVFRQHWERNATFFSSL
                     RSCGYAGEFFSYFKSVGLNDVLNVFQQVANVGTKAIDTVATSTTKVIDSSAKTLVNVV
                     DDTGRVVTNLTDGVGNITQLAKYAIPVLIGGVVIFVGAYAYKNFIKGDGRISVPTPGG
                     IIKV"
     gene            complement(3241..4032)
                     /locus_tag="PK281_08740"
     CDS             complement(3241..4032)
                     /locus_tag="PK281_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49474.1"
                     /translation="MEGNNGEGIKQAVQDLKDRGVKTIVLDVYRPNGSAKMFDRSFTI
                     KNEVEDKLAPINSNQNFGSLPLFQSPPKQSLRKPIETKPMESWKDYALQTERDKVAKL
                     EAETAKLKTENKILDTKVRDFEKEMIRKEHEVQSLAKSVESKSGLNGFVDKAVENPAM
                     MNMLAGIASRLMGIPGEPMNGGQPMLLQPGESQEHSNSKVDQYITNIKTWLYKQPEQV
                     QDHFYELVYQLTNSKDVTGAMQKFTNLLRKPTPARLEDEVKLGYQ"
     gene            complement(4137..5156)
                     /locus_tag="PK281_08745"
     CDS             complement(4137..5156)
                     /locus_tag="PK281_08745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49475.1"
                     /translation="MPGGVVKANKDRVVASIPDRDKLAGSWTTSNTGQGVIVQNAFKD
                     FPNTNEGDPTVETNSYAYYKWTGNSFIKISEGEGLDINFNAVYDSVVPDIPSLNTLVL
                     SQSWGIPEMGKGVLVINAFKDYPNLGQGDATVKSISNAYYKWTGNTFIKISEGEGLDI
                     EITGDNVAINISARDTLAATWSSADAGKGVLVINAFKDHPSPGQGDPTVESISNAYYK
                     WDGAQFLKISEGEGLDKSDKIFKTFEFSSDLFTEDTISVRGRIGTLSQSTTNELLSVS
                     YESRLDAAPGWTTHANLAALQTWIDTNATGNNITGTIFWIKCLATYKGNAIGEAENLF
                     GYQYL"
     gene            complement(5159..5392)
                     /locus_tag="PK281_08750"
     CDS             complement(5159..5392)
                     /locus_tag="PK281_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49476.1"
                     /translation="MNKVVKTSIDGSIDFTIQNVGSQKIFFGFTKDSKPDIPLDAGDF
                     SSFPLYRSCEEWTGDLFLMFGTSGGAVMVIKTI"
BASE COUNT         1428 a         1183 c         1043 g         1817 t
ORIGIN      
        1 cggtcagtga tgccgaatgt cttggctaat ttctctaacg taattttgtt ctgcttcgca
       61 ccagattgca gtagggatga aacggcctga acaaaatctt cctttatcat ttaataatct
      121 gattcaatca ctgtacagga tttcactgtg atactatttc aataaggctt tgtttttatc
      181 gtggcttcct ttgcttgaac caaagtagta accggaaatt cctacggtta atgtttcaac
      241 cattgtactg attctaacaa aatgttctcc ttccactttc atccaaatgg aggcataaac
      301 aattgcgcca aatgcagaaa gaatgataag gcccgtgagg tacatgagaa aatcccatcg
      361 tccggtttta gcgagatcaa cttctcgtac tcttgcgctc gaacggtctt gaatctccaa
      421 ttcaatttgt ttcaatgaat ttgtttccaa agcttcgaaa tgtctgttca cttctttttc
      481 aacttccagt tgtgctttga tgcgctcctc tttgttggta attaatttat cgagtacagt
      541 acccacagaa gtaactactt cttttgcccc gcttccaaat agtttagtta agatgttcat
      601 tggagtattc attgatatat ctcgtatgct tttaaaatct caataatgcg ttgtttcaaa
      661 atccttgttt cggacaaagc tttttcgatc atgtctttgg actccaatgt ctccgcaata
      721 gcgaattgtg aatagtggtg atgatgtggt cgcttcagca gatttaagaa gttcatagaa
      781 cgatttactg cctctaaatt caataacaga agtccttcta tccggcaaat ctctggcaac
      841 aaatctttgt ggtaaacccg attgatgagc cgttgaaatt cctgttgatt gtgcagtagc
      901 aattccttct gttggaaaat aaccatgcgt tctcgaacat actttcggaa caggtacaac
      961 aggccaaaaa atcctgttga taagccaatc aaaactccaa gtaaaaacac gttcacccgt
     1021 tttcattact atcttttccc ttttgcttta tgtaaatgat gaatgccgct acgccattag
     1081 agccagccgt tccgaaaaac atgagcatgt attcttccca tgagtattgt acctggtaaa
     1141 gtgttttgat aacgatcatg taaatgagca tgaggtaaga acccaatgcc atgtagaggt
     1201 atgccacttc ccatacttcg tttaaatcga gtttgctgtt ctcaccttcg ataccggcac
     1261 gaagccagtt acgaatgcgc ttgctgaaaa tggaagagag taaaggaata acgataatca
     1321 ccattacgaa tagcgtaatg aatattttta ggtatggcac aaacttgtag aatgcttctc
     1381 taatttcact catgattatt tcagtttaat tttttcgttt tcctttttct tgcaaaatct
     1441 tctttactgt taatgcagcc aggacagata aggcaaaacc tgcaaaccaa tcgagcactt
     1501 taccattacc atgtgaagtt gtattgagtt gattaactat tcctgaattg ttctgatctg
     1561 taatatcaat caccattcgc tttggaatat caagattgat aacctggcca atccttatca
     1621 aatttgggtt ggctatttta ttgtgctttg ccaaatcttg ccatagtgta atatcaccca
     1681 gtgcatcacg tgctatttta gaaagtgtat cacccacttt tacgatgtag ttcatgtcgc
     1741 taatttttta aaatcttcgc tgcgtgaaat cacgtaaaga acgattgcgc ctgcggctaa
     1801 cagtccgaga gcagcaccgc ctgttgtggg acttaatagt gttgggaatt ttgccagacg
     1861 tgccatccat ccttctctaa aaacttcctg tgaaggattt ttagcgatga gtgattccag
     1921 caagttctta cgttcactca ataacgcatc gtgaattttt gcagcgtttc ctttattgat
     1981 tgtggctatg gttgttgtcc ccattttgcc atcagccgga acgccaacaa tcctctgaat
     2041 aaacttgatc gctgttgtct gactatgaac atggaagtca tacaacagat tggcaatttc
     2101 ctgattaaca atttcagtaa gtcgaattga attccatcga ttcaaataaa agtctgttac
     2161 taaatagtcg atagagggaa acttactatt gagttttatt ggttttccac tgagcttcct
     2221 tttctcaaaa tcaataaacg tccaaccttg ccaagcctga ttataattac gggctatgcc
     2281 tgcgtatgtt tctcctccct tgtccgctgc aacattggcg tagcctccct catttggaag
     2341 tatgtaggtt ttgtatgcga tgtcgaaatt ggccatgatc ttaaacttta attattcctc
     2401 ctggtgttgg tactgaaatc cggccatcgc ccttaatgaa atttttgtaa gcatacgcac
     2461 caacaaaaat tactacacct ccaatcaaaa ccggaatagc atatttggca agctgagtaa
     2521 tgttgcccac gccatcagta agattggtta ctactcttcc ggtgtcgtcc actacattca
     2581 caagtgtttt tgcagagcta tcaattacct tcgtagttga agtagccacc gtatcgattg
     2641 ctttcgttcc aacattggca acttgctgga atacgttaag aacatcgttg agacctactg
     2701 atttaaagta ggagaaaaat tcaccagcat atccgcatga tcgaaggcta ctaaaaaatg
     2761 ttgcgttacg ttcccaatgc tgacgaaata cctgattact taattcaatg gcttgctctt
     2821 tggtgtattt ctttttgctt gcaaatcttc ccgctataaa tccttcaacc aatctcttat
     2881 gaaagatcat ccaatcagcg caaccccaaa caacttctcc cttgtgggca agagaggtat
     2941 tgggttgtgc aaacttgtat gtcttcggat cgtaaactac cggaacgatg gttactccga
     3001 gtgagcttac ttgttttcgt ttcagtgcat ggttcagaat tatttccata acactaaatc
     3061 agatgttggt acgatttgat tttttgattt acgccaacct tactggactt gttagccagt
     3121 attaccaggc tatcgcgatt gaggttacga gcaagaatct gcaatgcctg ttctttgtca
     3181 cttcgctctt tgtcggtagt cgagtttagt ttaattgtaa ttgctgactc catactgttt
     3241 ttattggtaa cctaatttta cttcatcttc caatcttgca ggtgttggct tccttaacag
     3301 gttggtgaat ttttgcatcg cacctgttac gtcttttgaa ttagtgagtt gatacaccaa
     3361 ttcataaaaa tgatcttgca cttgttcggg ctgtttatag agccatgtct taatgttggt
     3421 gatgtactga tctaccttag aattagaatg ttcctgggat tcgcctggtt gaagtagcat
     3481 aggttgtcca ccattcatgg gttcaccagg tattcccatc aatcttgagg cgatacctgc
     3541 gagcatattc atcatggctg gattttctac cgccttatct acaaatccgt taagaccaga
     3601 tttactttct actgacttcg ctaaactttg aacctcatgt tctttgcgta tcatttcctt
     3661 ttcaaaatcc ctgaccttag tatcgaggat tttgttttct gttttcaact ttgcggtttc
     3721 cgcttccagc ttcgctactt tgtcgcgctc tgtttgtaaa gcataatctt tccagctttc
     3781 cattggcttt gtttctattg gctttctcag ggattgtttg ggtggagatt gaaacagtgg
     3841 aagcgaaccg aagttttgat tggaattaat tggtgctagt ttgtcttcaa cttcattttt
     3901 gattgtgaac gagcggtcaa acatttttgc gcttccgttc ggtcggtaca catccagtac
     3961 gattgtctta acacctctgt ctttcaaatc ctgtacggct tgcttgatac cttcgccatt
     4021 attgccttcc atcttgcgat gaactttcgt tttctttcca tcgggcaatt cgtagcttat
     4081 gtccactact tttctgttgc cacttatata ggttagtatt tcgctgcgca tgtcgtttac
     4141 aagtattggt atccgaaaag attttcagct tcgccaatgg cgttgccttt ataggtagca
     4201 aggcatttaa tccaaaagat tgttcctgta atattgttac ctgttgcatt cgtgtcaatc
     4261 catgtttgca aggcagcgag gttagcgtgt gttgtccaac caggagcggc atctaatcgt
     4321 gattcgtagg acacacttaa gagttcattg gtggtagact gactcaatgt tcctattctc
     4381 ccacgaacgg aaatggtatc ttcggtaaac aggtcgcttg aaaactcaaa cgtcttgaaa
     4441 atcttatcgc tcttgtccag tccttcgccc tcgcttattt tcaaaaactg tgcgccatcc
     4501 catttgtaat aggcgttcga aatactttca acagttggat cgccctgtcc tggagagggg
     4561 tgatctttga aggcattaat aaccagtaca cctttaccgg catcggcact gctccacgtt
     4621 gcagcgagtg tgtctctggc agaaatgttg atagccacgt tatcgcccgt aatctctatg
     4681 tctagtccct cgccttcact tattttgatg aaggtatttc cagtccattt gtaataagcg
     4741 ttcgaaatac ttttaacggt tgcatcgcct tgacctaaat tgggataatc tttgaaagca
     4801 ttgataacaa gtacaccttt acccatttcc ggtatacccc aactttgtga tagcactaaa
     4861 gtgttaaggg acggaatgtc aggtactacg ctatcataaa cagcattgaa atttatatca
     4921 agcccttcac cttcgcttat ttttataaat gaattgcccg tccatttgta gtacgcatac
     4981 gaattggttt caacagtcgg atcgccttcg ttagtatttg gaaaatcttt aaaagcattt
     5041 tgtacaataa caccttgacc cgtattggaa gtagtccaac tgccagcgag cttatcgcga
     5101 tcaggaatgc ttgctacaac cctgtcttta tttgccttta caactcctcc tggcatgact
     5161 agatagtttt gataaccatt actgcaccgc cagaagtacc aaacatcaga aagagatctc
     5221 ccgtccattc ttcacatgaa cgataaagcg gaaaggaaga aaaatcgcca gcatccaatg
     5281 gtatatcggg tttactgtct ttggtaaatc caaagaagat tttttgactc cctacgttct
     5341 gaatcgtaaa gtcaatcgag ccatcaatgc tcgtcttcac tactttattc aaatcagaaa
     5401 aatagcttac gtccggtttg cgccttattg cttcgttcgg cggttggctg tgaggaacgc
     5461 attaaaaggg g
//
LOCUS       DAOOQW010000159         5388 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_18285_length_5388_cov_4.824302, whole genome shotgun sequence.
ACCESSION   DAOOQW010000159 DAOOQW010000000
VERSION     DAOOQW010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5388
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18285_length_5388_cov_4.824302"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..482)
                     /locus_tag="PK281_08755"
     CDS             complement(<1..482)
                     /locus_tag="PK281_08755"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOY49477.1"
                     /translation="MHGSDLDVLNETKGCSTYKKGQVIFNAGAYPHGLFCVYEGKIKI
                     FRIGDEGKEQIVRLARGGDILGYRALLSGDKYSSSAEALDDCRICFIPQSAFLKLVEG
                     NGILSMQLMKLLSEDLKHAEHRITDIAQKPVRERVAEALLYLQQTYGLDQDGVTISVT
                     "
     gene            744..2498
                     /gene="aspS"
                     /locus_tag="PK281_08760"
     CDS             744..2498
                     /gene="aspS"
                     /locus_tag="PK281_08760"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013073921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOY49478.1"
                     /translation="MLRTHTCGELRSSHIGTAVELCGWVAKSRDLGGMTFIDLRDRFG
                     ITQLVFNMDDDSALCERARKLGREFVLRVKGEVRERSSKNAQLVTGDVEIRVLDFEIL
                     NASKTPPFTIDDDTDGGDDLRMKYRYLDLRRNVVRKNLELRHNLAKAIRAYLDNAGFL
                     EVETPVLIKSTPEGARDFIVPSRMNPGEFYALPQSPQTFKQILMVAGFDRYYQIVKCF
                     RDEDLRADRQPEFTQIDCELTFIEQEDILNIFEGLVRELFVAVKNVTFEDFPRMTYAE
                     AMEQYGNDKPDIRFDMRICDFTSSTRNSGFVVFDQAEYVAGIAATGCSEYTRKQVDEL
                     TDWVKRPQLGAGGLIYIRMNLDGSVKSSVDKFFSEEQLRAFCMQAKAQPGDLVLILAG
                     AKTRTQKALSELRLEMANRLGLRPKDTFAPLWVLDFPLLEWDEENARYHAMHHPFTSP
                     KPEDIPLLETDPGKVRANAYDLVINGVEVGGGSIRIFDKGLQKNMFTLLGFSDEDAQA
                     QFGFLMNAFEFGAPPHGGIALGFDRLCSIFGGAESIRDFIAFPKNNSGRDMMIDSPSE
                     VNPDQLRELQISVQKKTV"
     gene            2495..3076
                     /locus_tag="PK281_08765"
     CDS             2495..3076
                     /locus_tag="PK281_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49479.1"
                     /translation="MRNTLALIVIALILVFAAPQHSFAQYKHSEAFEKGITVIDLNTS
                     FGTYRDKAFVTQRMPIFLGAEYGINNDISAGLFGGWNQRTYKTINSFAYDVNYLYYGG
                     RFSLHLTSFLRKKTILKFNPHKVDVYGTIYVGRQVARQQTFSGTGYLDSGATNIFGGY
                     VGAKVYTNYQVGFLVELGAGPYGIMNLGVCYKF"
     gene            complement(3073..3516)
                     /locus_tag="PK281_08770"
     CDS             complement(3073..3516)
                     /locus_tag="PK281_08770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49480.1"
                     /translation="MRILLRTRVKNTSVSKTIPLFNRELFLKLSPPFPKSELLRFDGC
                     KKGDLVQLRLRLFFTWHYWDSEIVEDSQTDNSWQFVDSGTRLPFFLSGWLHKHSLVQS
                     GNDVLVIDDIQFKAYNRFFELLSYPALWIQFAFRKPIYRKELGKA"
     gene            complement(3518..4417)
                     /locus_tag="PK281_08775"
     CDS             complement(3518..4417)
                     /locus_tag="PK281_08775"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF002187.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HOY49481.1"
                     /translation="MTNSTTRLTYTLNPPQMPSLNLRVTIDSNSGFCFGVVYAIEMAE
                     DVLDEQGYLYCLGDIVHNDEEVNRLKAKGLRIINYDTFATLQNEKVLIRAHGEPPSTY
                     QTAIQNNITLIDATCPVVLKLQNRIRGSYDKKETIFLIGKKGHAEVTGLMGQINNEGV
                     VFSDISELDGQTLPSEITLYTQTTKSKQKFRETRKAFEDRGIKVNAHDTICSQVSDRD
                     TELRVFASSYDKILFVSGTKSSNGKVLFQVCKDVNPETYFISKVDELDSSWFDDNQTV
                     GICGATSTPMWLMKDVKAALEAL"
     gene            complement(4414..5248)
                     /locus_tag="PK281_08780"
                     /pseudo
     CDS             complement(4414..5248)
                     /locus_tag="PK281_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008464902.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
BASE COUNT         1552 a         1075 c         1119 g         1642 t
ORIGIN      
        1 aatgtaacac taatggttac accatcttga tcgagtccat aagtttgttg gagatagagc
       61 aaagcctcgg caacacgttc acgtacaggc ttttgagcaa tatcggtaat gcggtgttcg
      121 gcatgtttta aatcttccga caacaatttc atcaattgca tcgataaaat accatttcct
      181 tctaccagct ttaagaatgc agattgtgga ataaaacaaa tgcggcaatc atctaaggct
      241 tcagcacttg aggaatattt atcgccactt aataatgccc gataacctaa gatatcaccc
      301 ccacgagcta aacgaacaat ctgctctttt ccctcatcac caatccggaa aattttaatt
      361 tttccctcgt aaacacagaa taaaccatgc ggataagcac cagcattaaa gattacctga
      421 ccttttttat aggtactaca acctttcgtt tcatttaaaa cgtctaagtc agatccatgc
      481 atatcgcaaa aaacagaacg agatctgttt gaacaatgct cacaatctgg gtggtcgttt
      541 tttcgaagca tacctttaaa ttggactgta aatatactac tctcctcata atagctcaac
      601 ttaaaatcgt tttctgttgg ttgattttaa tacagactga gtaaaaactg ttcctgttaa
      661 aaaaacacat cgcttttttt cctttaaggc ctttctccga cttaccttcg cacggcatta
      721 ttttttggac gattatattt accatgctaa gaacacacac ctgcggagaa cttcgctcat
      781 cacatatcgg aactgctgtt gaattatgcg gctgggttgc caaatcacgc gatttaggcg
      841 gaatgacttt tatcgatttg cgtgatcgtt ttggaattac gcaattagta ttcaacatgg
      901 atgatgattc tgctttgtgt gaacgtgcac gtaaacttgg acgtgaattc gttttgcgcg
      961 tgaagggcga ggttcgcgaa cgttctagca agaatgcgca attagttacc ggcgatgtag
     1021 aaatccgagt actcgatttt gaaattctca atgcgtcaaa aactccacct tttactatcg
     1081 atgatgatac tgatggtggt gatgatttac gcatgaaata tcgttacctc gatttacgcc
     1141 gtaacgtggt tcggaaaaac ctagaactcc gtcataatct tgctaaagca attcgtgctt
     1201 atcttgataa tgcgggtttt ctcgaagtgg aaactccggt tctgataaaa tctactcccg
     1261 aaggagcacg cgattttatt gtgccatctc gtatgaatcc gggtgagttt tatgcgcttc
     1321 cacaaagtcc gcaaacattc aagcaaattc tcatggtagc tggtttcgac cgctactacc
     1381 agatagtaaa atgtttccgt gatgaagatt tgcgcgccga ccgtcaaccg gaatttactc
     1441 aaatcgactg cgagcttact tttattgagc aagaagacat cctcaatatt ttcgaaggtt
     1501 tagtgcgtga gttatttgta gcggtgaaga atgtcacctt tgaagatttt cctcgaatga
     1561 cctatgctga agcgatggaa caatacggga acgacaagcc cgatattcgt ttcgatatgc
     1621 gcatttgtga tttcacttca agtacacgca actccggttt tgtggtattt gatcaagccg
     1681 aatatgtagc tggaattgca gcaacaggtt gttctgaata cacgcgtaaa caggtagatg
     1741 aattaaccga ttgggtgaaa agacctcaat taggagcagg tggactaatc tacattcgca
     1801 tgaatctgga tggtagcgtg aaatcatcgg tcgataaatt cttctccgaa gagcagttac
     1861 gtgcattttg tatgcaggca aaagctcaac cgggagattt ggttttaatt cttgctggtg
     1921 ctaaaacacg tactcaaaaa gccttgagtg aattacgcct tgaaatggcg aatcgcttag
     1981 gtcttcgccc gaaagatacc tttgctccat tatgggtttt agattttcca ttgttagaat
     2041 gggatgagga aaatgcgcgt tatcatgcta tgcatcaccc atttacttct cctaaaccgg
     2101 aagatattcc tctgttggaa actgatccag gtaaagttcg cgccaatgct tacgacttgg
     2161 taattaacgg agttgaagta ggtggaggtt caattcgtat tttcgacaaa ggcttgcaaa
     2221 agaacatgtt cactttactt ggttttagcg atgaagatgc tcaagctcag tttggtttct
     2281 tgatgaatgc gtttgaattt ggagctcctc cacatggcgg tattgcttta ggattcgatc
     2341 gtttatgttc catctttggt ggagctgagt cgattcgtga ttttatcgct ttccctaaaa
     2401 ataattcggg tcgcgatatg atgatcgatt ctccttctga agtaaatcca gatcagcttc
     2461 gcgaattgca aatctcagtt caaaaaaaga cggtatgaga aatactcttg cattgattgt
     2521 tattgctcta atactggttt ttgcagctcc tcagcacagc ttcgctcaat acaaacattc
     2581 agaagcgttt gaaaaaggaa ttacagttat cgacctcaat actagctttg gaacttaccg
     2641 cgataaggcg tttgttaccc aacgaatgcc gattttcttg ggtgctgagt acggaatcaa
     2701 taatgatatc agtgccggat tattcggagg atggaatcaa cgcacctaca agactatcaa
     2761 tagttttgct tacgatgtaa attacctcta ttatggaggg cgctttagtt tacacttaac
     2821 cagcttttta cgcaagaaaa ctatcttgaa attcaatccg cacaaagtag acgtgtacgg
     2881 tacaatttac gttggaaggc aagttgctcg tcagcaaaca ttctctggta ctgggtattt
     2941 agactcggga gcaacgaata tttttggtgg ctacgttgga gcaaaggtgt ataccaatta
     3001 ccaagttggg tttttggttg aactaggcgc agggccttat ggaataatga acttaggcgt
     3061 ttgttataaa ttctaagctt ttccgagttc tttccggtag atgggttttc ggaatgcgaa
     3121 ttgaatccat aaagcaggat aagaaagcaa ctcgaagaaa cgattgtacg ctttgaattg
     3181 aatgtcgtca ataactagta catcattccc cgattgaact aggctatgtt tatgaagcca
     3241 gcccgaaagg aagaagggaa ggcgcgttcc tgaatcgacg aattgccaag aattgtcagt
     3301 ttgtgaatct tcaacaatct cgctatccca ataatgccaa gtaaaaaata ggcgtagacg
     3361 taattggact aaatcacctt ttttacatcc atcaaatcga agtaattcag attttggaaa
     3421 cggaggacta agcttaagaa ataattccct attaaatagg ggaatggttt tgctaacgga
     3481 ggtattttta acgcgcgttc ttagtaaaat ccgcatccta aagcgcttcg agtgccgctt
     3541 ttacatcttt catcagccac atgggtgttg atgttgcccc acaaatccca accgtttgat
     3601 tatcgtcgaa ccaagaagaa tcgagctcgt ccactttact gataaagtat gtttcgggat
     3661 ttacatcctt acaaacctga aacagcactt ttccattcga cgatttggtg ccacttacaa
     3721 ataagatttt atcgtacgaa ctagcaaata cacgtaattc tgtatctcga tcacttactt
     3781 gactacaaat agtatcgtgt gcattgactt tgattccgcg gtcttcaaaa gcttttcgag
     3841 tttcacggaa tttttgtttg ctcttagtcg tttgtgtata gagtgtaatt tcggatggaa
     3901 gtgtttgtcc atctaattcc gaaatatcag agaaaacaac gccttcatta ttaatctggc
     3961 ccatcaaacc tgtcacttca gcatgacctt ttttaccgat aaggaaaatt gtttccttct
     4021 tgtcgtatga gccacgaatt cggttctgta gttttaaaac aaccggacaa gtagcatcaa
     4081 ttaaagtgat attattctga atggccgttt ggtaggttga tggaggttct ccgtgggcac
     4141 gaatgagtac tttttcattc tgaagggtcg caaacgtatc gtaattgatg attcgcaagc
     4201 cctttgcttt gagacgattt acttcttcgt cgttgtgaac gatatcgcct aagcagtata
     4261 aatagccttg ttcatctagc acatcctcag ccatttcgat ggcatacacc acaccaaagc
     4321 agaaacccga attggagtca atcgtaaccc gaagattcaa tgagggcatt tgtggaggat
     4381 ttagcgtgta agtcaagcga gtggttgaat tggtcaaaga aggttgagcg aatggcgcac
     4441 gtatgaacta agaattaggg agtatttcca aatgtttggg attcggattc gttcctctag
     4501 tatgcgagaa ggagaagtat tggtgatttt ctttaggagt gcgaaatagt aaacgtatgc
     4561 cacgtaaacg cctaatcttg cgccttttgg aagagcttta attccagcga aaccatctag
     4621 aaaatctttc tgaatatctt tttctatcga acgtttgcac tcttcattaa actcactcat
     4681 gttgaggtga ggaaagtaaa cacgaccaag aatctggtag tcgttactta aatcgcgtag
     4741 gaaatttatc ttctgaaatg ccgatcctaa ccgcattgca ggagttttca atgcttgata
     4801 ctgtgcctcg tttccttcgc aaaaaaacac gtaaacacat taggccaaca acttcggcag
     4861 agccaagtat gtatgtttcg taggattgct gatcatattc tcggtcgctc aaatccattt
     4921 ccatactctt aagaaatgta tcaatcaact cggtttcgat cttgtattta ttgactactt
     4981 cctgaaagct atgtaggatt ggatttaaac ttattcctcg ttcgatggct agataggtat
     5041 cgcgacgaaa ttcagctaat aataattccg catcctcacg attgaaggtg tctacaattt
     5101 catctgcaaa gcggacaaag gcatacaaag catatacagg atcctgaaac cgggttgcta
     5161 aagagcgaat tcccagcgta aaggatgtgc tgtaacgata agtggtgatt cggctacagc
     5221 gtatgcttac ggcattgtat tctttcatag atgtgtattc taaaccctca agttagaaat
     5281 tataaccttt tagcatggtc attaattgac gacgagcaag aaaaattcta ctcttaacag
     5341 tgccaatagg aagatctaga taatcagcta tctctttgta cttgtatc
//
LOCUS       DAOOQW010000160         5345 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_18476_length_5345_cov_4.693195, whole genome shotgun sequence.
ACCESSION   DAOOQW010000160 DAOOQW010000000
VERSION     DAOOQW010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5345
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18476_length_5345_cov_4.693195"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(159..626)
                     /locus_tag="PK281_08785"
     CDS             complement(159..626)
                     /locus_tag="PK281_08785"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOY49482.1"
                     /translation="MSIEQEVQQVKFKSEYQKASINLMYTVSSITHHHLQVLKKFGLT
                     TPQFNILRILRGQYPKATTVNDLINRMIDKASNASRIVEKLRAKKLVERSVSSNDRRA
                     VDVKITEAGLELLSKIDHIEDEFFFGTSSLTVDEAKMLNYLLDKGRETKIRTK"
     gene            complement(709..2103)
                     /locus_tag="PK281_08790"
     CDS             complement(709..2103)
                     /locus_tag="PK281_08790"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY49483.1"
                     /translation="MKSRYLILLITVISLSLNAQNQNRHISPFAIKNTIRQTHDRSVP
                     DWRPGQERFSTWDSKGSVWNLVNTNDYSYDNSYLTTSMVNYESGMQLDERISYTYNAS
                     GAQESEIIETWNGSAYVNGSKSVTLYSATGFETGYENYDWDGTNWVLSDGQRRVDVYS
                     GGNLVTATSEAYDNGIGSWGAYSDYTLTYNANNKIDEVVIRTAVSTPGVLENEFKFSC
                     GYSASAIQPDTIFLYQWDGSTWILNQRAIDIVWNGEGLILIEIEPANYILQDFMNSDW
                     INSERQTTTITGLNKLILVELSDGESWFDDNRTTIEYDQFDNLTIDKFETFDIDTWTI
                     ENFSSYTNTYDGESRLITIEAQYWNNINLALQNSYRREFLDFVDISGISDLNSSDFSL
                     FPNPANAEIVLKGKFNHTSNYSVFDISGKCVLNGSLSGSDLNTINIQQLEAGYYLIRI
                     SGTEGLVTKSFVKN"
     gene            complement(2192..3163)
                     /locus_tag="PK281_08795"
     CDS             complement(2192..3163)
                     /locus_tag="PK281_08795"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008469040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response
                     exonuclease"
                     /protein_id="HOY49484.1"
                     /translation="MDLLQFTEKGIYCPPADVFLDPSRKVNRALISHAHSDHARGGSL
                     AYLAHHDSAEVLRLRLGKNLPLETIGYGEPVVINGVKFSFHPAGHTIGSAQIRVEYKG
                     EVWVYSGDYKLENDGFSQPFEPVKCDVFITESTFGLPVYRWKPQALIFDEIHSWWKGN
                     AAKGICSVMHGYSLGKSQRLLFHLDQSIGPIYVHDAIEKTNEVIRKSGFELPNAQLLS
                     ADSSPEQLSRALVITPFIAPDSPMMQLLGSYSLGIASGWMGIGGAKRMGAADRGFVLS
                     DHADWPSLNIAVKESGASRIIVNHGFTEAYSLWLSSIGFQAEAARIV"
     gene            3221..3619
                     /gene="mce"
                     /locus_tag="PK281_08800"
     CDS             3221..3619
                     /gene="mce"
                     /locus_tag="PK281_08800"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011711178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HOY49485.1"
                     /translation="MNKIEHLGIAVRDLSKAQELFNLLLGKESYKVESVEREKVNTLF
                     YQLGESKIELLESSDPDGTIAKFIDKRGEGFHHVAIAVDDIRAEMQRLKNAGFELLND
                     EPKPGADNKIICFLHPKSTGGLLVELCQEK"
     gene            3702..4991
                     /locus_tag="PK281_08805"
     CDS             3702..4991
                     /locus_tag="PK281_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013751001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HOY49486.1"
                     /translation="MKYQAIDSALYIENRRRFTAKMKPNSIAVFVSNDEMPRSADATY
                     FWRQNPDLFYLSGIDQEQTFLILFPDCPNPKMREVLFLRKTNEHIAVWEGHKYTKEEG
                     KAASGIENILWTESFESTFHVLMTYASTIYLNQNDHDRASTVVPYHELRFAQQVQAKY
                     PLHHIERSSPIMHALRAIKSPLEVAAMQTACNITDKAFRRVLGFVKPGVWEHEIEAEI
                     IHEFIRNRATGHAYNPIIASGPSACVLHYVENNRQCKDGDVILLDFGSEYGNYNSDLS
                     RSIPVSGRFTARQRAVYDAVLRVMKYATNMLRPGTKYDNYVAQVGLAMEEELIQLGLL
                     NAAEVKNQNPDSPLYKKYFMHGVSHYLGIDVHDVGYRYAEFQAGMVFTCEPGIYIPEE
                     GLGIRLENNILLTENGNVDLMGHIPLEAEEIEDLMNR"
     gene            complement(4992..>5345)
                     /locus_tag="PK281_08810"
     CDS             complement(4992..>5345)
                     /locus_tag="PK281_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013597940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOY49487.1"
                     /translation="IHVVDISHPSFGDHIAAVNETLTDIKSMDKPILMVFNKIDAFTY
                     VKKDEDDLSPILRENFSLDDWKKSWMAKNNAPAIFISATEKENIEPFRKLLMEEVKKV
                     HSIRYPNYLKGDQEY"
BASE COUNT         1660 a         1153 c          951 g         1581 t
ORIGIN      
        1 agccgccgtg cgagaagtac acatccttga cgcccggcac ctgcttttgc agcatcagcg
       61 ctctcgccag tattaacgga acttcctcca cacgatgaca atatcaacat ggaaattacc
      121 gatgacatta agaatactcg tttcattttt cgatttgttt atttggttct gattttagtt
      181 tcacggcctt tatcgagcag gtaattcaac attttagctt catccacagt tagcgaactc
      241 gttccaaaaa agaactcgtc ctcgatatgg tcaattttac tcaacagttc caaacctgct
      301 tcagtaattt tcacatcaac agcacgacga tcattcgaac taactgaacg ttccaccaat
      361 ttcttagcac gaagtttctc aactatgcga gatgcatttg aggctttgtc aatcattcga
      421 ttaatcaaat cattgactgt tgtagctttc ggatactgcc ctcgtaagat tcgtaaaata
      481 ttaaattgag gagttgtaag accgaatttc ttaagcactt gcaagtgatg atgcgtgata
      541 ctgctaaccg tatacatcaa attgatagag gctttttgat actcgctctt aaacttgact
      601 tgttgaactt cttgctctat tgacatatcg acaaatttat gtaaacacat taaatgtacc
      661 tacataagtg ttctttttta tcctcgccaa agcattaaaa ggtgaatatc agttttttac
      721 aaatgactta gtaactaaac cttctgttcc ggaaatgcga ataagataat atccagcttc
      781 caattgttga atatttatgg tgtttagatc tgagccactt aagctaccat tcaacacaca
      841 tttaccagaa atatcaaata cagaatagtt gcttgtatga ttgaatttgc cttttagcac
      901 tatttcagca ttcgccggat tgggaaacaa actaaaatca gaactattca aatcagaaat
      961 acctgatata tccacaaaat ctaaaaattc acgacgatag ctattctgta atgccaagtt
     1021 gatgttgttc cagtattgag cttcaattgt aatcaaacga gattcaccat cgtacgtatt
     1081 tgtgtaagaa gaaaaatttt caattgtcca agtatcaata tcaaatgttt cgaatttatc
     1141 tatcgtaaga ttatcaaatt gatcatactc aatcgtagtg cgattatcat caaaccaact
     1201 ttcaccatcc gataactcaa ctaaaattaa tttattcaat cctgttatag tagttgtttg
     1261 acgttcagaa ttaatccaat cggaattcat gaaatcctga agtatgtagt tagctggttc
     1321 aatttcaatg agaataagtc cttcaccatt ccaaacaata tcaatagccc gttgattcaa
     1381 aatccatgta cttccatccc attgatatag gaaaattgta tcaggttgga tagcagaagc
     1441 agaatagcca cagctaaact taaactcatt ttctaggacg ccaggagtag aaacagcagt
     1501 tcgaattaca acttcatcaa tcttattatt tgcattataa gttagtgtgt aatcagaata
     1561 tgccccccaa gaaccaatac cattatcata tgcttcacta gttgcagtta ccaaattacc
     1621 tcctgagtaa acatctactc tacgctgacc atcagataaa acccaattag ttccatccca
     1681 gtcataattt tcatatccag tttcgaatcc agttgctgaa tacagtgtaa ccgatttaga
     1741 accgttcaca tatgcacttc cattccatgt ttcaataatc tccgactctt gtgctcctga
     1801 agcattgtac gtatagctta tacgttcatc cagttgcatt cccgattcat aattcaccat
     1861 actcgttgtc aagtatgaat tatcatagct gtaatcgttg gtattgacta aattccacac
     1921 actacctttc gaatcccaag ttgagaatct ctcttgtccg gggcgccaat cgggtactga
     1981 gcggtcatgc gtttgacgaa tggtgttttt aatcgcaaaa ggactaatgt gtcgattttg
     2041 attttgtgcg tttagcgaca gtgaaatcac tgtaattaga agaattaggt agcgactttt
     2101 catgatgatg ggtttatata gaacaaagct actaagcaat atgtgagaat atcttcgaat
     2161 gggtaaatat cttaacagtt gattgtttat gctatacgat acgtgcggct tctgcttgaa
     2221 agcctatact tgaaagccat aaagagtagg cttcggtaaa tccatgattc acgataattc
     2281 ggcttgcacc actttctttc acggcaatgt tgagtgatgg ccaatcagca tgatccgata
     2341 gaacaaaacc acggtcggca gctcccattc gtttggcacc accaattccc atccaaccgg
     2401 aagcaattcc caatgaatac gaacctaaaa gctgcatcat gggcgaatcg ggtgcaataa
     2461 atggggtaat cactaaagcc ctacttaatt gttcaggtga tgaatctgca ctcaacaatt
     2521 gagcattggg taattcaaat ccacttttac gaattacttc atttgtcttt tcaatcgcat
     2581 cgtgaacgta aattggacca atggattgat ctaaatgaaa cagcaaacgt tgcgatttac
     2641 cgagtgaata accatgcatc acactacaaa ttccctttgc tgcatttcct ttccaccatg
     2701 aatgtatttc atcaaaaata agtgcttgtg gtttccaacg atacacaggt aatccaaagg
     2761 tcgattcggt aatgaaaaca tcacatttta cgggttcaaa cggttgagaa aatccatcat
     2821 tttcaagttt gtaatcgccc gaataaaccc aaacttcacc cttatattca acccgaattt
     2881 gtgccgaacc aattgtatgt cctgccggat gaaaggaaaa tttcacccca ttaatcacta
     2941 ctggttctcc ataaccaatc gtttctagtg gcagattttt tcccaatctc aagcgtaaaa
     3001 cttctgccga atcgtgatgt gccaaataag ccaatgatcc tccacgagca tgatcactat
     3061 gggcatgact aataagtgca cggttcactt ttcgtgatgg atcaaggaaa acatcagctg
     3121 gcggacaata aattcccttc tcggtaaatt gtaacaaatc catcgctgta acattacaaa
     3181 aaacacccat cgatatgctt cctaaatgta tttttgtagc atgaataaaa tcgagcatct
     3241 aggaattgca gttcgcgact tatcaaaagc acaagaactc ttcaacctgt tattaggaaa
     3301 ggaatcgtat aaagtagaaa gtgtggagcg tgaaaaagta aacacccttt tttaccaact
     3361 aggagaaagc aaaattgagc tcctcgaaag ttccgatcca gatggtacca ttgccaaatt
     3421 catcgataaa cgaggagaag gatttcacca tgtagctatt gccgtagatg atattcgagc
     3481 cgaaatgcaa cgcttgaaaa atgccggttt tgaattactc aatgatgaac ctaaaccagg
     3541 tgccgacaat aaaataatct gctttcttca tcccaaatca acgggtggat tactcgtgga
     3601 actttgtcag gagaaataaa aatatcaaaa cataacacct aagtggtatg ttaaattcgg
     3661 agaattcttc taattttccg gcctcacctg accaaagcat catgaaatat caagcaatcg
     3721 attctgcact ttatattgaa aaccgtcgtc gctttactgc gaagatgaaa ccgaatagta
     3781 tcgccgtttt tgtttctaac gacgaaatgc cacgcagtgc tgatgctact tatttctggc
     3841 gtcaaaatcc cgatttattt tatttatcag gtatcgatca ggaacaaact tttctaatcc
     3901 tcttccccga ttgccctaat cccaaaatgc gtgaagtctt gtttttacgc aaaaccaatg
     3961 aacacattgc ggtttgggaa ggacacaaat acaccaaaga agaaggaaaa gcagcatcag
     4021 gaatagagaa catcctctgg accgaaagtt tcgaatcgac ctttcatgtc ttgatgacct
     4081 atgcgagcac tatttatttg aatcaaaacg atcatgatcg ggcaagtaca gttgtacctt
     4141 accatgaatt acgatttgca caacaagttc aggcaaaata tccccttcat catatcgaac
     4201 ggagctcacc aatcatgcat gcacttcggg caatcaaatc tccactcgaa gtggcagcta
     4261 tgcaaacagc ctgtaacata accgataaag cattccgtcg tgttctaggc tttgttaaac
     4321 ccggtgtttg ggaacatgaa attgaagcag aaatcattca cgaattcatc cgcaatcgcg
     4381 caactggtca cgcctataac ccaataattg catcaggtcc atctgcttgc gtgttgcatt
     4441 atgtcgaaaa taaccgtcaa tgcaaagacg gtgatgtaat cctgctcgac tttggttccg
     4501 aatacggaaa ctacaattct gatttgtccc gcagtattcc tgtaagtgga cgattcacgg
     4561 ctcgtcagcg tgcggtttac gatgcagttc ttcgggtgat gaaatatgcc accaatatgc
     4621 ttcgtccggg tacgaaatac gataattacg tagctcaggt cggactagct atggaagaag
     4681 agttaatcca acttggattg ctcaacgcag ctgaagtgaa aaatcagaat cccgattctc
     4741 cgctctacaa aaaatacttc atgcacggtg tatcccatta cttaggcatc gatgtgcatg
     4801 atgttggata tcgttacgct gagtttcaag cgggcatggt gtttacatgc gaaccaggta
     4861 tttacattcc agaagaaggt ttgggcattc gtctcgaaaa taatatcctc ctaactgaga
     4921 atggtaacgt tgacctcatg ggccacattc cgctcgaagc tgaggaaatc gaagatttga
     4981 tgaatcgtta atcaatattc ctgatcacct ttcaggtagt tcggatagcg aatcgaatgc
     5041 acttttttca cctcttccat caataattta cggaaaggct caatattctc tttttctgtg
     5101 gcagatatga agatagctgg tgcattgttc tttgccatcc agcttttctt ccaatcatca
     5161 agcgagaaat tctccctcaa tattggagat aaatcatcct cgtccttctt cacataagta
     5221 aacgcatcta tcttattgaa aaccataaga atgggcttat ccatgctttt gatatcggtc
     5281 aaagtttcat ttactgcagc tatatgatcc ccaaaacttg gatgcgaaat atcaaccaca
     5341 tgaat
//
LOCUS       DAOOQW010000161         5343 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_18485_length_5343_cov_6.430976, whole genome shotgun sequence.
ACCESSION   DAOOQW010000161 DAOOQW010000000
VERSION     DAOOQW010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5343
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18485_length_5343_cov_6.430976"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /locus_tag="PK281_08815"
     CDS             <1..711
                     /locus_tag="PK281_08815"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HOY49488.1"
                     /translation="KQQQKPLFQLWNTEWKNLPLSDYTLGIDEIPVMLHKMDEHPWPI
                     YKVKLGNKKDIEIIQALRAHTNAPLRIDVNGGWTLTEAKEKIQELSIYGIELIEQPLS
                     KDERHLMPQLKAQSTIPLIADESCVREEDVEKCADGFHGINIKLTKCGGISPALRMIK
                     TARKLGLKVMIGNMNESSIGTSAIAHFLPQLDFVDMDGPLLLEGDLASGLELQNGIAK
                     LSNEPGLGILPTFEMTIA"
     gene            729..2129
                     /locus_tag="PK281_08820"
     CDS             729..2129
                     /locus_tag="PK281_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017842930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HOY49489.1"
                     /translation="MSTIQPADLTFFCEVLGNEFVLYDEENLQAHSHDETEDLRYLPE
                     VVLRPETPEQISQILKHCNLRGIPVTTRGGGTGLSGGALPIHGGVVVSMSRFNRILEI
                     DERNLQATVEPGVITQVFQEAVIEKGLFYPPDPSSRGTCFIGGNLAHCSGGPRAVKYG
                     VTRDYVLDLEVVLASGDIIRTGAKVLKNSTGYNLTQLMVGSEGTLGVITKVVFKLLPY
                     PRMNKVLLAPFRSPLDACAAVSAIFREGIIPSALEFMERDAIDWTLHFRTDINIQIGE
                     EEQAHLLIEVDGNDEETILKDIERIVPVLEAHNCMEILFAESEAQKTDLWKLRRSVAE
                     AVKSNSTYKEEDTVVPRAELPVLLSGVKEIGAKYGFKSVCYGHAGDGNLHVNIIKGDM
                     SEAQWKEDVTKGIREIFTLVVSLGGTLSGEHGIGLVQQPYMPIAFSNVQLELMRGIKN
                     VFDPKGILNPGKIFNV"
     gene            2347..2712
                     /locus_tag="PK281_08825"
     CDS             2347..2712
                     /locus_tag="PK281_08825"
                     /inference="COORDINATES: protein motif:HMM:NF037170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOY49490.1"
                     /translation="MQNNILWHDLTVDDAAKVSEFYEKVAGWERQSFDMGGYNDYVMS
                     PEECEDPIAGICYARGSNKDIPPQWLMYVNVENLDKSLEAVTRLGGKVLGDKRKMGDD
                     GKFYCLIQDPAGAYMMLCG"
     gene            complement(2709..4499)
                     /locus_tag="PK281_08830"
     CDS             complement(2709..4499)
                     /locus_tag="PK281_08830"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HOY49491.1"
                     /translation="MKKIFLLVIIFSTPFIIKAQYSVDTNLTVNQYVEKLLGPGIAFS
                     NAHIYGDRQAIGYFTGAGDMLSIQRGILLTTGYAAKTNDLVALQQNGDDNNGLSAIPE
                     LDSMGGIDTRDGLIFQFDFVPQSNLISFQYIFASQEYNEFVCSQFNDAVAIFLSGPGI
                     VGEQNLAVVPISGEPISVNTGNNGNFIDTSNGFCTGENAEYFDSIVPPNLIFDGYTVK
                     MNAFAEVLPCVNYTLRFIIADVGDDIYDSAIFLKGLTESTNIGITAVINDEDQLIYED
                     CSSLKLTFHNTDTLSSDYVFHYSLSGTAENGMDYTTIPDSVIILAGTNTTDIYLSVFQ
                     DNQNEGIETITFTYQTVCGDFISEFQISDLPALEIVQNPIEVVCAGKGPIQLSANLIQ
                     GVPPYTYSWKNSFSSESFLSVNPLVETMYRLYVTDRCGTTDSTDFIVPVFPLPEPITL
                     YYTSSNSSIWFDEPVSNDSIFWVFNEASKQLITNPNLTLSSTGSYSVYRVDSNGCSSI
                     SNAILFDSNSSTSELFLIYPSPAKDNLSIALVDVSIFEIYDLAGRLIRNQVLLEGIQT
                     ISVADIPQGIYLVRIQNSSGTGTAKIFITH"
     gene            complement(4512..>5343)
                     /locus_tag="PK281_08835"
     CDS             complement(4512..>5343)
                     /locus_tag="PK281_08835"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY49492.1"
                     /translation="EINVEIYQDNEAETAETLSFNYSNTCNIYTTEFQIVDKPELLFE
                     TIPVSEICTGQGSINLHVEVTQGILPFTYAWLNNGSTEPDLTVEPLDSTLYRVYVTDL
                     CGTTDSVDFVVNVNPQPDSIELSFDTLTSSISFDEPAGIVMSYWLLDESNYQVVSEPN
                     LTLTAAGTYSVYCELSDGCTVYSNSIYFDPLILEIEELDKFPFKLYPNPADDYFSISV
                     IEDCNLEIYDLAGKLLSKLPIKAETQTISTTNFSSGMYLLKLRSNKGEKSIKLNIEKY
                     "
BASE COUNT         1698 a         1070 c         1061 g         1514 t
ORIGIN      
        1 aaacagcaac aaaaacctct tttccaattg tggaataccg aatggaaaaa tctacctctt
       61 agcgattaca cccttgggat agatgaaatc cccgtgatgt tgcacaaaat ggatgaacat
      121 ccttggccta tttataaagt aaaattggga aacaaaaaag atattgaaat catccaagct
      181 ttaagagcac atactaacgc tcctttgaga attgatgtaa acggtggctg gactttgact
      241 gaagccaagg agaaaattca agaactaagc atttacggaa ttgaactcat cgagcaacca
      301 ctttcaaaag atgagcgtca tttaatgcct caactcaaag ctcaatcaac tattccgctt
      361 attgctgatg aaagttgtgt gcgtgaagaa gatgttgaaa aatgcgccga tggatttcat
      421 ggaatcaaca tcaaactcac caaatgtgga ggaatttcac ctgccttacg catgattaaa
      481 accgctcgaa aactgggctt aaaagtcatg ataggcaaca tgaatgaatc gagtattggc
      541 acatctgcca ttgcccactt tctacctcaa ctcgattttg tggatatgga tggaccactc
      601 ttgttagaag gagatcttgc atccggatta gaactgcaaa acggaatagc taaactttcc
      661 aatgagccgg gtttgggtat tcttcccacc tttgaaatga cgattgctta acctcttgat
      721 cgatgtccat gtcaactata caaccagccg atttaacttt cttttgcgag gtacttggaa
      781 acgaattcgt gctatacgat gaagaaaacc tgcaagccca ttcgcacgac gaaaccgaag
      841 atttacgcta cttacccgaa gttgtccttc ggcccgaaac acctgaacaa atcagccaaa
      901 ttctaaaaca ctgcaatctt cgcggaattc ctgtaactac tcgtggtggt ggaaccggtt
      961 tgagtggtgg agcacttcct attcacggcg gtgtagtagt gtcgatgagt cgatttaacc
     1021 gcattctcga aatcgatgaa cgcaaccttc aggcgacagt tgaaccgggt gtaattacgc
     1081 aagttttcca agaagcggtg atagaaaaag gattgttcta tccacccgat ccatcgagtc
     1141 gaggcacctg ttttattggt ggaaatctag cgcattgctc gggcggacca cgtgcggtga
     1201 aatatggagt aacgcgcgat tatgtgctcg atttagaagt ggttttagct tctggagata
     1261 tcattcgaac aggtgccaaa gtccttaaaa actcgaccgg ctataacctc acacaactca
     1321 tggttggcag cgaaggaacg ctgggtgtga ttaccaaggt agttttcaaa cttcttccct
     1381 atccgcgtat gaataaggtt ttgctcgcac cttttcgttc gcctttagat gcatgtgctg
     1441 cggtttcggc tattttccgc gaaggcatta ttccatcggc cttggaattt atggaacgcg
     1501 atgccatcga ctggacctta catttccgca ccgacatcaa catccaaatt ggagaggaag
     1561 aacaagcgca tttgctcatc gaagtagacg gtaacgatga agaaacgatc ctcaaagata
     1621 tcgaacgcat tgttccggtt ctcgaagcgc ataactgcat ggagatttta tttgccgaat
     1681 cggaagcgca aaaaaccgac ctctggaaac tccgccggag cgttgccgaa gccgtaaaat
     1741 cgaattcaac ctataaagaa gaagataccg ttgttccgcg cgccgaattg ccggttttgc
     1801 tttcgggtgt taaagaaatt ggagcaaaat atggtttcaa atcggtgtgt tacggacatg
     1861 cgggcgatgg aaatttacac gtcaacatca tcaaaggcga tatgagcgaa gcccaatgga
     1921 aagaagacgt caccaaaggc attcgcgaaa ttttcactct cgttgtttcg ctcggtggaa
     1981 ctttgagtgg cgaacacgga attggtttgg tgcaacagcc ttatatgccc atcgcttttt
     2041 cgaatgttca attggaactc atgcgcggaa tcaaaaacgt tttcgatccc aaaggcatcc
     2101 tcaacccagg aaagattttc aacgtgtaaa gcatttgggc gtgccctttt cgcacacaca
     2161 ttccctcgca aaaaggtcgc gctttacgct atactccaca tcgccagcca tagctttacg
     2221 cgaaggctgg cgatgcgggg tgccgctgca atcgctcacg cgggggggat cgtaatcatg
     2281 catcatcccc tttgtgaatc gttattcgat tatcttgcat ccccggattg aaatccgaga
     2341 ctatttatgc aaaacaacat cctctggcac gacctcaccg ttgacgatgc cgcgaaagtc
     2401 agcgaattct acgaaaaagt agctggctgg gaacgccaaa gcttcgacat gggcggatat
     2461 aacgattatg tgatgtcgcc cgaagaatgc gaagatccta ttgcgggaat ctgttatgca
     2521 cgaggcagca acaaagacat tccgcctcaa tggctcatgt atgtgaatgt agagaacctc
     2581 gataaaagcc ttgaagctgt tacacgtttg ggtggaaaag tcttaggcga caaacgcaaa
     2641 atgggtgacg atggcaaatt ctattgcctc atacaagatc cagcaggagc gtatatgatg
     2701 ttgtgtggtt agtgcgtgat gaaaattttt gcagtccccg ttccagatga gttttgaatt
     2761 ctcactagat aaattccttg cgggatatct gctaccgaaa tagtttgaat tccttctaat
     2821 agaacttgat ttcgaattag tctaccagct aaatcataaa tttcaaaaat gctaacatca
     2881 acaagagcga tggaaagatt gtccttagct ggacttggat aaatcaggaa gagttcggaa
     2941 gtcgaactat tggaatcaaa caaaattgca ttggaaatac tcgaacatcc attcgaatcg
     3001 acccgataaa ccgaatagct tcctgtagat gataaagtca aattgggatt tgttattagt
     3061 tgtttacttg cttcattaaa cacccaaaaa atagaatcat tacttactgg ttcatcaaac
     3121 catatactgg aattagaact agtatagtaa agtgtgatgg gctcaggtaa tggaaaaaca
     3181 ggtacaataa aatcggtaga atcggtggtt ccacaacgat cggtgacata tagccgatac
     3241 atggtttcta ctagtggatt aacagataag aacgattccg aactaaaaga atttttccaa
     3301 gaatacgtat aaggaggaac accctgaatt aggtttgcac taagttgaat agggcctttc
     3361 ccggcacaca ctacttcgat aggattttga acaatttcta atgctggtag atccgaaatt
     3421 tgaaattcag aaataaaatc cccacaaaca gtttgatagg taaaagtgat cgtttctatt
     3481 ccttcgtttt ggttatcctg aaatacactg agataaatgt cggtagtatt tgttcctgct
     3541 aaaatgatca ccgaatcggg aatagttgta taatccattc cattttcggc tgtacctgaa
     3601 agcgaataat ggaaaacata atcggaagat aaggtatccg tattgtgaaa cgttaattta
     3661 agagaggagc aatcttcgta aattaattga tcctcatcat taattacagc agttattcca
     3721 atgtttgttg attcagttaa tccttttaaa aatatagcag agtcataaat atcatctcca
     3781 acatctgcaa ttatgaacct caaggtgtag ttcacacaag gtaaaacttc agcaaatgca
     3841 ttcatcttta cagtgtatcc atcgaaaatc aaattcggtg gtacaatgga gtcaaaatat
     3901 tcagcattct ctcctgtaca aaaaccattt gaagtatcta taaaatttcc attatttcca
     3961 gtattaacgg aaataggttc tcctgaaata ggaacgactg ctaaattttg ctcacccaca
     4021 ataccgggcc cagataaaaa aattgcaacc gcatcgttaa attgcgaaca aacaaattcg
     4081 ttatactctt gagaggcaaa tatgtattga aaagaaatca agttggattg aggaacaaaa
     4141 tcaaattgga aaattaatcc gtctcttgtg tcaatacctc ccattgaatc tagttcaggt
     4201 attgccgata acccattatt atcatctcca ttttgttgaa gtgcaacaag atcattagtt
     4261 ttagcagcat atccagttgt caataaaatt cctctttgaa tacttaacat atctcccgct
     4321 ccagtaaaat atcctattgc ttgtctatcg ccgtatatat gagcattact aaatgcgata
     4381 cctggaccta ataatttttc cacatattga tttacggtca ggtttgtatc aacagaatat
     4441 tgggctttta ttataaatgg agttgaaaat ataatgacaa gtaaaaaaat ctttttcatt
     4501 aaatattgga attaatactt ttcaatattt aatttaatag acttctcccc tttattagaa
     4561 cgaagtttta acaaatacat tccagaacta aaatttgttg ttgaaatagt ttgtgtttct
     4621 gctttaatag gcaacttaga aagcagcttc ccagctaaat catatatctc caaattacag
     4681 tcttcaatta ctgaaataga gaaataatca tcagctggat tgggatatag tttaaaagga
     4741 aatttatcta attcttcaat ttctaaaata agagggtcaa aataaatgga gtttgaataa
     4801 acagtacatc catccgataa ttcacagtaa acagaataag taccagcagc agtcaatgtt
     4861 agattgggtt ccgaaacaac ttgataattc gactcatcta acaaccaata actcattaca
     4921 atcccagcag gctcatcaaa actgatagaa gaagtgagtg tatcaaaact taattctatt
     4981 gaatcaggtt gcgggttgac attaaccaca aaatcgactg aatcagttgt accacataag
     5041 tcggttacat aaacacgata tagcgttgaa tctaatggtt ctaccgtcaa atcaggctct
     5101 gtgcttccat tattcaacca agcataggta aatggtagaa ttccttgggt tacttcaaca
     5161 tgcaaattga tggagccttg tccggtacaa atttcagaaa ctggaattgt ttcgaataat
     5221 aattcaggtt tatcaacaat ttgaaattca gttgtatata tattacacgt atttgaatag
     5281 ttaaatgata gtgtttcagc tgtttcagcc tcattatcct gatatatttc tacatttatt
     5341 tca
//
LOCUS       DAOOQW010000162         5309 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_18639_length_5309_cov_4.808907, whole genome shotgun sequence.
ACCESSION   DAOOQW010000162 DAOOQW010000000
VERSION     DAOOQW010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5309
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18639_length_5309_cov_4.808907"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(55..642)
                     /locus_tag="PK281_08840"
     CDS             complement(55..642)
                     /locus_tag="PK281_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008637278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOY49493.1"
                     /translation="MKAEMELNEGFWRERYLTGETAWDMGHVSQPLKEYFDQLTNKEL
                     KILIPGAGNAWEAAYLWEQGFKNVWICDIVAEPLARFQERHTDFPAAQLLHVDFFHLQ
                     DAFDLIIEQTFFCALDPKLREAYAQKMSTLLNPNGRLVGLMFDDPKNDDRPPFGGKKE
                     DYQVLFESYFHEVRIEPCYNSIAPRAGKEVFVRMK"
     gene            complement(632..1024)
                     /locus_tag="PK281_08845"
     CDS             complement(632..1024)
                     /locus_tag="PK281_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009745733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOY49494.1"
                     /translation="MGIQLTSIHHVAIICSDYARSKWFYTELLGLNILAEHYREERQS
                     WKLDLGLGENYVIELFSFPEAPARLSRPEACGLRHLAFAVADLEKAIAFLHTARWETE
                     AIRVDEFTGKRFCFFADPDGLPIEFYES"
     gene            complement(1028..2440)
                     /gene="rlmD"
                     /locus_tag="PK281_08850"
     CDS             complement(1028..2440)
                     /gene="rlmD"
                     /locus_tag="PK281_08850"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007093268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HOY49495.1"
                     /translation="MGRSRSQTPQVYHNLEITDLASEGMGIARHEGMVVFVEQAVTGD
                     VVDVQITRKKSGFRQGKVIHFHQRSALRVEPKCEHFGICGGCKWQVISYADQLKHKAK
                     QVKDALTRIAKVPLPEIAPIHGSATEYYYRNKLEYTFSASRWLTEEEIGSKDEFSDRA
                     ALGFHIPGRFDKVLKINNCYLQPDPSNAMRLFVEKRAKELGISFFNLMEQTGVMRNLL
                     LRNNSKGEWMVCLSVTDALPEVFTLLDDLKAAFPQIHALLYTINTKRNDTISDLDIFV
                     HSGAGFIEEEMEGLRFRIGPKSFYQTNSEQAYELYKHTRAFAGITKDDVVYDLYTGTG
                     TIALFVAGLAKKVVGVEYVPEAVADAKINAEVNGVENVSFFAGDMKDVLVEGFFETHG
                     KPDVIITDPPRAGMHDDVTKRLIESGARRIVYVSCNPATQARDLILLDQAYTVVAVQP
                     VDMFPQTTHVENIVVLDRKN"
     gene            2587..3837
                     /gene="rocD"
                     /locus_tag="PK281_08855"
     CDS             2587..3837
                     /gene="rocD"
                     /locus_tag="PK281_08855"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009751384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine-oxo-acid transaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HOY49496.1"
                     /translation="MRSSETTNTNTLHGNDYFIHLEEEHTAHNYHPIPVVLERGQGVY
                     VWDVEGKRYYDFLSAYSAVNQGHSHPKILAALIEQAQTLALTSRAFHNKAFAEFGAFI
                     TSYFGYDRFLPMNTGAEAVETALKLCRKWAYQKKGLPENAAKILVCSGNFHGRTISIV
                     SASTDPDARKEFGPYTPGFEVIEYNSIAALEKALQDPHVAGLLIEPIQGEAGVMVPDE
                     GYLSACHALCQKHNVLFIADEIQTGIARTGKLLAIDHENIHADILILGKALSGGMYPV
                     SGVLASHEIMLCIRPGEHGSTYGGNPLASSIATAALTVVKEEKLAENAAVQGEKLRVA
                     LRAIPSSRIQLVRGKGLLNAIVIEEKDGVTAWDICIRLMENGLLAKPTHGHIIRFAPP
                     LVINDEQLSECIQIIHDTLMSFDA"
     gene            3830..4327
                     /locus_tag="PK281_08860"
     CDS             3830..4327
                     /locus_tag="PK281_08860"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOY49497.1"
                     /translation="MLKKQTLIRVLLGLLLLAGGTHLYFRFRVAPALLLPEIQMQTLN
                     GESVTATQGQATILIFFATWCPDCRRELPVLSSLENRITDLNIRIILVSDESVEKLQQ
                     FSSTIPSRFELVKLKGKFSDNGIHTLPTSYLFCQDGSVYLKKTGAIEWTSELLEQFTK
                     DCPKK"
     gene            4592..>5309
                     /locus_tag="PK281_08865"
     CDS             4592..>5309
                     /locus_tag="PK281_08865"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOY49498.1"
                     /translation="MDIKSRISGFIICFFTLILASCDTQNAKKDVQNGSISVQEAFKL
                     DTILTKRYQSIAVKIDSISGINNQDSLLRAYFLDHEQEYQPEELIQSMSLYEKYFSTS
                     KSAVGFANEERAIVYLRTSQLDTADYYATKAINAYTESGERKSLARCYNVLAGIKGFQ
                     GRLDESFNNQFQALDIYKALADSNGIYETVRELGNTCYRQHLFERALTLYNKSLTYYI
                     AQQDTFMQADLYNSIGNALHQ"
BASE COUNT         1585 a         1152 c         1031 g         1541 t
ORIGIN      
        1 aattcgtaat tcgtaattcg taattcgtaa ttcgtaattc gtaattcgta attattattt
       61 cattctcaca aacacctctt tcccagccct tggagcaata gaattataac acggctctat
      121 tcgtacttca tggaaatagc tctcaaataa aacttggtaa tcctccttct ttccaccaaa
      181 aggcggacga tcatcatttt taggatcatc aaacatcagc cctaccaatc gcccatttgg
      241 attcaataaa gtggacattt tttgcgcata agcttcacgt agtttgggat ccaatgcaca
      301 gaaaaaagtc tgctcaatga tgagatcaaa cgcatcctgt aagtgaaaaa aatcaacatg
      361 cagcaattgt gcagcgggga aatcggtatg acgttcttgg aatcgtgcca atggctctgc
      421 cacgatatcg caaatccata cgttcttaaa tccttgttcc cataaataag ccgcttccca
      481 agcatttccc gctccgggaa ttaaaatctt cagctccttg ttcgttaatt gatcgaagta
      541 ttctttcaag ggttgagata cgtgccccat gtcccaagct gtttcgcctg ttagataacg
      601 ttctctccaa aaaccttcat ttaattccat ctcagctttc ataaaattca atgggtaaac
      661 catcgggatc agcaaaaaaa caaaagcgct ttccggtaaa ttcatccaca cgaatggctt
      721 ctgtttccca acgggcagta tgtaaaaagg caattgcttt ttctaaatca gccaccgcaa
      781 aagctaaatg tctcaatcca caggcttccg ggcgactcaa gcgcgcaggt gcttctggaa
      841 aagaaaacaa ctctatcaca tagttttcac ccaaacctaa atcgagcttc caggattggc
      901 gctcttcacg gtagtgctca gcgagtatgt tcagtccgag caattcagta taaaaccatt
      961 tcgatcgggc ataatcggaa cagataatgg ccacatgatg aatggatgtt agctgaatac
     1021 ccatgactta gtttttacga tctaatacta caatgttctc tacgtgtgtt gtttgtggaa
     1081 acatatctac tggttgcact gcgaccacag tatatgcttg atcgagcaat attaaatcgc
     1141 gtgcttgagt ggctggatta caactcacat atacaatacg acgcgcgcca ctttctatca
     1201 atcgcttggt tacatcatca tgcattccag cacgtggtgg atcggttata atcacgtctg
     1261 gttttccatg cgtttcgaaa aagccttcca ccaacacatc cttcatatca ccagcaaaaa
     1321 agctcacatt ttccacgccg tttacctcag cattaatctt agcatcggcc accgcttcgg
     1381 gcacgtattc cacaccaact acctttttcg ccaatccggc tacaaacaag gctatcgttc
     1441 ctgttccagt gtataagtca tacaccacat cgtctttggt aattccagca aaagcgcggg
     1501 tatgcttgta tagctcatat gcttgctctg aattagtttg atagaatgat tttggaccaa
     1561 ttcggaatct cagtccttcc atttcttcct caataaatcc agcacccgaa tgcacaaaaa
     1621 tgtctagatc cgaaatggta tcgtttcgtt tggtgttgat ggtgtacaac aaggcatgaa
     1681 tctgaggaaa ggcagctttt aaatcatcca acaaggtaaa aacctcaggc aaagcatcag
     1741 ttacactcaa gcaaaccatc cattcgcctt tgctgttatt acgtaacaac aaattgcgca
     1801 tcacgccagt ttgctccatt aagttgaaga aagaaatccc caattctttc gcacgttttt
     1861 ccacaaacaa tcgcatcgca ttgctcggat cgggttgcaa ataacagttg ttgattttga
     1921 ggactttatc gaaacgtccc ggaatatgaa atcccaaagc agcacgatca ctgaattcat
     1981 ctttactgcc gatttcttct tccgtcaacc aacgacttgc cgaaaatgtg tactccaatt
     2041 tattgcggta atagtattca gtagcagaac cgtgaattgg cgcaatttcc ggaagaggaa
     2101 ctttggctat gcgtgttaat gcgtctttta cttgcttggc cttgtgtttc aactgatcgg
     2161 cataggaaat cacctgccat ttacatcctc cgcaaattcc aaaatgttcg catttaggtt
     2221 caacacgtaa agcacttcgt tgatgaaaat gaatcacttt cccctgacga aagccacttt
     2281 ttttgcgagt aatttgcaca tcaactacat cgccagtaac cgcttgctcc acaaaaacca
     2341 ccatgccttc gtgacgagca atgcccattc cttcggaagc taaatcagtg atttcgaggt
     2401 tgtgatatac ttgtggcgtt tgcgatcgtg atcgtcccat gctttttaaa attttgcgca
     2461 agttcaggaa attgggcgaa taggcgtaat aaactaactt tgtcgggctt tcaaattttc
     2521 gatcagcttt tcccaaacag cgaaaatgga tgtttctcaa tatccaaagc tcacaaactc
     2581 aattttatgc gctcatccga aaccactaac accaacacac ttcacggaaa tgattacttc
     2641 attcacctcg aagaagaaca taccgctcac aattatcatc caattccggt tgtcttagag
     2701 cgtggacaag gtgtttatgt ttgggatgtg gaaggcaaac gctattatga tttcctttct
     2761 gcctattcag ctgtaaatca aggacattcc cacccaaaaa tccttgcagc tcttattgaa
     2821 caagcacaaa cgttggcatt gacctctcgt gcgtttcaca ataaagcttt cgcagaattt
     2881 ggagctttca tcacctccta ttttggttat gatcgttttc tacccatgaa tacaggtgcc
     2941 gaagcggttg aaacagcctt gaaattgtgt cgcaaatggg cctatcagaa aaaaggactt
     3001 cctgaaaacg cggctaagat tttagtgtgc tcggggaatt tccacggaag aaccatttcg
     3061 attgtttctg cttctaccga tccagatgct cgtaaagaat ttggaccata cacgcctggt
     3121 ttcgaagtga ttgaatacaa cagcattgca gcactagaaa aagcgcttca agatccgcat
     3181 gtggcaggtt tgctcattga accgattcaa ggagaagcag gtgtgatggt tcctgatgaa
     3241 ggctatttga gcgcttgtca tgctctctgt caaaaacata atgtgctatt cattgcagat
     3301 gaaatccaaa caggaattgc acgaacagga aaactgcttg ccattgatca tgaaaacatc
     3361 catgcagata ttctcatctt aggaaaagca ctttcgggtg gaatgtatcc tgtttcgggc
     3421 gttttagcga gtcacgagat catgttatgc attcgtccgg gcgaacatgg ttcaacttat
     3481 ggcggaaatc cattggcatc gagcattgcc actgcagcct taacggtggt taaagaagaa
     3541 aaactagctg aaaatgcagc agttcaagga gagaaacttc gtgtggcgct tcgcgccatt
     3601 ccaagttcac gtatccaatt ggttcgtggt aagggattgt tgaatgcaat cgtaatagaa
     3661 gaaaaagatg gcgtaacagc atgggacatt tgtatccgat taatggaaaa cggattgcta
     3721 gctaaaccca ctcacggaca tattatccgg tttgcacctc cactggttat aaatgatgag
     3781 caattgagcg agtgtattca aatcatccac gatacgttaa tgtcgtttga tgcttaagaa
     3841 acaaacgctt attcgcgttc tactaggatt actattacta gcaggtggta cacatttgta
     3901 tttccgcttt cgggttgcgc cagcattatt attgcccgaa attcaaatgc aaacactaaa
     3961 tggtgagtcg gttacggcta ctcaaggaca agccaccatt ttgatattct ttgcaacctg
     4021 gtgtcccgat tgtcggagag aattacctgt attgtcgagc ttagaaaacc gcattacaga
     4081 tcttaatatc cgcattatac tagttagcga tgaatcggtt gagaaacttc aacaattcag
     4141 ctctactatt cctagtcgtt ttgaattagt gaagcttaaa ggaaagttca gtgataacgg
     4201 catacataca ttacctactt cctacctgtt ttgtcaggat ggctctgttt acttaaagaa
     4261 aacaggggcg attgaatgga cttctgaact attggaacaa ttcacgaaag attgtcctaa
     4321 aaagtaaaac ggcaaaagtc aaatcttggt agattgcttt tgccgtcttt tcgccgaagc
     4381 gtatgtgaag atggtttgtg ataggcatta cgaaaatgcc ttaacaagta tacaaggtaa
     4441 aattaaccta ggtcaaaatt cggtaggtac aaatagaggg acaacactca tatatacgtg
     4501 gtcaataccg tagacaaagt ttgcatctcc ctgtaaatga atagattact gattagtatt
     4561 ttggattatt atttcgcatc ttgcggatga aatggacata aaaagtagga tttctggatt
     4621 cattatttgt tttttcaccc ttattctagc atcgtgtgat acacaaaatg ctaagaaaga
     4681 tgttcaaaat ggctccatat ctgttcaaga ggctttcaaa ttagatacaa tacttactaa
     4741 gcggtatcaa tcaatagcag ttaagatcga ttctatttca gggattaata atcaggattc
     4801 tctattacgg gcgtactttt tagatcatga acaagaatat cagcctgaag aattaatcca
     4861 atccatgtcg ctatacgaga agtacttttc tacttcaaaa agtgcagttg gttttgctaa
     4921 tgaagaaaga gccatcgtat acctgcgtac gagtcagtta gatacggcag attattatgc
     4981 gaccaaggct ataaatgcct atacggaatc gggagaacga aaatctttag cacgttgtta
     5041 taatgtttta gctggcatta aaggatttca agggcgattg gatgaatcat ttaataatca
     5101 gttccaagca ttagacatat ataaagcact cgctgatagc aatggtatat atgaaacagt
     5161 gcgagagcta ggtaatacgt gttacagaca acatttattt gaacgcgctt taacactcta
     5221 taataaaagt cttacctatt atatagctca acaggataca ttcatgcaag ccgatttata
     5281 caactcaatt ggcaatgcac ttcatcaat
//
LOCUS       DAOOQW010000163         5256 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_18923_length_5256_cov_4.574697, whole genome shotgun sequence.
ACCESSION   DAOOQW010000163 DAOOQW010000000
VERSION     DAOOQW010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5256
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18923_length_5256_cov_4.574697"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..4165)
                     /locus_tag="PK281_08870"
     CDS             complement(<1..4165)
                     /locus_tag="PK281_08870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49499.1"
                     /translation="MKKHLFIFILWLVHVPLMGQLPLQVHVNIAPPYSATYQAYVNNP
                     TSLTVTITNSTTETYQIYLAGSLENTSTGARVFTDPSNPWTAPGLVIMPGASVFTGPD
                     ANIFNDIGDIQTVNVDPASYIDGILPEGNYQLCLRAYNYFSAEHEALSPEAVLNNGCF
                     EFSISFPTAPVPDYFNCEQTFPATQPQNLTFSWSPSMSIPLGSTVMYRFKLVYYPEGI
                     DIQSALESTSPLDMVYEEENIITQSIFYGADKPPLIEGKRYAWRVEAYDPQGQIVFQN
                     NGISTPCYFFYNNAAAHQGNFSMDFPLSGDTLPWNFMPMVSRFSPYNENYNRFLSDFS
                     ISGSNGSSLDFQRDLSWPNGPVSGQSNATQQEIYSAQAVFVGVFKRHDENPLSFRFTH
                     GNLYEWEAGLELRVSNADDPTGNISGNFYSGMGMPRLNFPENADTLYLSEIASGSLDL
                     SFKTSNPPGKMFTLFDLLQTSDDINDNGIVINGTIDERYVSEVSRKADFSDVLQTKTE
                     RLGEGMNLANSATNACNQTCMVDELYKTVKHSLALTDTGWYYWRVKWLQNPLEENGEA
                     YLTSSVVRFYVADSLRLPDQLTNEEERPTPGSCIATSAIDPVPVDQRIPVNNITVDQL
                     VQVGLFTMKVTEINWSASRATGKGLIDIPFLKAPVRVVFSSIQINMQKRMFDGEVWAE
                     YDRQDIIPASWIQGAGMLAGLNSDEVNLLDEVLNSGARLTSQLLGSTPIGLPIGIDQV
                     IEGVPVLVGVVSLKFTPTRATLNAVCNVDFNDHPLNVAAGAMDVEFHPGGIGGPQGKA
                     ILYLAKDVRSKPYGLPGDSLIVEATRFEDNFHTVADSGTYICFDCLGFKSLNVKGKIK
                     FTTDQLVEDKPDGSIGDAKIEANFKFKYTTSQQIIARLNFNHSFQVAGAEGWGFQVHE
                     AWFDFSQIENPPNTNFPPNFGNIYDPITQQYSITNAWEGFYLKRATLGLPKMFKKFTG
                     NDRLVAGVEDLIIDRFRLTGKIRAYDILSIDEGNLGNWGYSLDTLKMDFVDGSFIQGG
                     FSGKLLIPSCDQPVLYSSLLKQTPQTNDWNYEFRAHPKDDMNAEFLKAKLELLETSII
                     SISVPDLNNIDIQAHLNGELSVNHDFDNIGQVDFKLVEFQDLCFKNSAPYLSCAAFRF
                     NSPQKYIGESESDETDLFSTDGIGSGFPINISDIELIHRSEDGKQLGGVEFNFNINLT
                     SSANSFSATARMAILGKIDLDAMRPQRWSYDQLDLDSVGVRGDVGAVKIEGYVKFYDD
                     DPIYGNGLKGKLFAEYKPRIQVLATGQFGNINGMRYWFVDAQTVFNPGIPLFAGINAY
                     GFGGGAWYNMSQTSPPPSAKDVKDTDPDKDKYVPGMTLSGAVYKPTPDVPLGLKATLI
                     FGTMGDEDVF"
     gene            complement(4162..>5256)
                     /locus_tag="PK281_08875"
     CDS             complement(4162..>5256)
                     /locus_tag="PK281_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49500.1"
                     /translation="LASLKGYTPKDNFVVGLTSRLSITRYFTWNIEAAGSVITDNVLA
                     EKLEVKNWSDSPFLSKLITPRIGTRLSLAGQSNLSFQARKFGMKFTFRQVDPGYSSLG
                     ALFLQSDVRSLTVDPSLKLMKGKLRIQASIGKEQDNLSRKKLFTSMRNIAAFNIAFAP
                     KPVYGIDASFSNYGLAQQAGLISINDTFRVAQVSRNAQLNQRLNFQNKQRVIGINLGV
                     GYQELKDLNTYSTNSMSENQIVFANLNSNYFRNRDKLSITSGLTYNKTMSFIGDRVGL
                     GPLIGIGKSFRNNKLKFNGNLMYQFIRVNQQASGSIANAYFTGSYQLKGGHQFQVNIN
                     FLHSSSTLQSFSEMRMMAGYVFAFHSKQKK"
BASE COUNT         1629 a         1054 c          944 g         1629 t
ORIGIN      
        1 taaatacatc ttcgtcaccc atggttccaa aaattaaagt cgctttcaag cctaatggaa
       61 catctggagt tggtttataa accgcaccac tcaaggtcat acccggcaca tatttatctt
      121 tatccggatc ggtatctttt acatctttag ccgaaggcgg aggtgaggtt tgcgacatgt
      181 tataccaagc tccaccgcca aatccgtaag cattaattcc agcaaataat ggaatacccg
      241 gattaaatac tgtttgagca tccacaaacc aataacgcat gccattaata tttccaaatt
      301 gaccggttgc taatacttga atacgtggtt tgtattcggc gaatagtttc ccttttaatc
      361 cattcccata aattggatca tcatcataaa attttacata accttcaatt ttaaccgccc
      421 ccacatcacc acgaacacca accgaatcta agtcgagctg atcgtatgac caacgttgcg
      481 gccgcatagc atctaaatcg atttttccta gaattgccat gcgagcagtg gccgaaaatg
      541 aattagctga gctagttaaa ttgatgttga aattaaattc cacaccacct aattgcttac
      601 catcctcgga acgatgaatt aactcgatat cggaaatgtt aattggaaaa ccagaaccaa
      661 ttccatcggt actaaataaa tcggtttcat cagattcact ttcaccaata tatttctgcg
      721 gactattaaa tcgaaatgca gcacaactta gatagggagc agaattctta aagcacaaat
      781 cttggaattc gactaattta aaatctactt gtccaatatt atcaaagtcg tgatttacag
      841 ataactcacc atttaaatgt gcctgaatat caatattatt taaatccggt accgatatag
      901 aaataatact ggtttcgagc agttctagct tagctttcaa aaattcggca ttcatgtcat
      961 ctttcggatg tgctctgaat tcataattcc aatcgtttgt ttgtggcgtt tgttttaaca
     1021 aactggaata aagaacaggt tgatcacatg atggaataag gagttttccg ctaaatccac
     1081 cttggataaa tgaaccatct acaaaatcca tttttagtgt atctaaacta tatccccagt
     1141 ttccaagatt tccttcgtca atcgataaaa tatcatacgc tcgaattttt ccagttaatc
     1201 gaaatcggtc gatgattaaa tcttccacac cagcgaccaa tcgatcattc ccggtgaatt
     1261 tcttaaacat tttaggtaat cctaaggtgg ctctttttaa ataaaatcct tcccaagcat
     1321 tggtaatact gtattgctga gttattggat cgtatatatt tccaaaattg ggagggaaat
     1381 tcgtattagg tggattttca atttgtgaaa aatcgaacca agcttcatgt acctgaaagc
     1441 cccaaccttc ggcacctgca acttgaaaag aatgattaaa atttaatcga gcaattattt
     1501 gctgactggt tgtgtattta aatttaaaat tcgcttcaat tttagcatca ccaatcgaac
     1561 catcgggttt atcttccact aattgatcgg ttgtgaattt gattttccct tttacattca
     1621 aggatttgaa acccaagcaa tcgaagcaga tatacgtacc agaatcggct actgtatgga
     1681 agttatcttc aaagcgagta gcttctacaa tgagtgaatc gcctggcaat ccatatggtt
     1741 tacttcttac gtctttagct aaatagagaa ttgcttttcc ttgtggaccg ccaattccac
     1801 ccggatgaaa ttccacatcc atcgcaccag cagctacatt gagtggatga tcattgaaat
     1861 cgacattaca tactgcattt agtgtggcgc gggtaggggt gaacttcaag gaaaccacac
     1921 ccactaaaac cggaacgccc tcaattactt gatcaattcc aattggcaat ccgataggcg
     1981 ttgatcctaa taactgactc gttaatcgag ccccagaatt caacacttca tctaataaat
     2041 tcacctcatc tgaattcaat cccgcaagca ttccagcacc ttgaatccac gaagctggga
     2101 taatatcttg tcggtcatat tcagcccaaa cttcaccatc gaacatgcgt ttttgcatgt
     2161 ttatctggat actagaaaaa actacacgaa cgggagcttt taagaacggt atatcaatta
     2221 atccctttcc agttgctcgt gaagcagacc aattaatttc agtcaccttc atggtaaaaa
     2281 gtccaacctg aacgagttga tctacagtaa tattattcac tggaattcgt tgatctacag
     2341 gaactggatc aatggcagaa gttgctatac acgaacctgg agttgggcgt tcttcttcgt
     2401 tggttaattg atcaggtaag cgaagactat cagccacata aaatcgaaca accgaactgg
     2461 ttaaatatgc ctcaccattt tcctctaatg gattctgcaa ccactttaca cgccaataat
     2521 accagccggt atctgttaag gctaacgagt gtttaaccgt tttatataat tcatctacca
     2581 tgcaggtttg attgcaggca tttgtggccg aattagctag attcattcct tctcctaaac
     2641 gttctgtttt agtttggaga acatcactaa aatcggcctt acgtgaaacc tccgaaacgt
     2701 aacgttcatc aatagttcca ttaattacta ttccattatc attgatatca tcacttgttt
     2761 gaagcagatc aaataaagta aacatcttac cgggcggatt ggatgtttta aaacttaaat
     2821 caaggcttcc gcttgcaatt tcagataaat acaaggtgtc ggcattttct ggaaaattca
     2881 aacgaggcat tcccataccc gaatagaaat ttcctgaaat atttcctgtt ggatcatctg
     2941 cattggacac acgtaattct aaacctgctt cccattcgta taaattaccg tgcgtaaatc
     3001 gaaaggataa tgggttttca tcgtgacgtt taaatacacc aacaaataca gcttgtgcag
     3061 aataaatttc ctgttgtgtg gcattacttt gtccgctaac aggaccattt ggccagctta
     3121 aatcgcgttg aaaatcgagc gaagaaccat tacttcccga aatactgaaa tcacttagga
     3181 accgattgta attttcgttg taaggagaaa accgtgaaac catgggcata aaattccacg
     3241 gtagtgtatc acccgataat ggaaaatcca tgctgaaatt accttgatgt gcagcagcat
     3301 tattgtaaaa gaaataacat ggcgtagaaa ttccattatt ttgaaatact atttgtcctt
     3361 gtggatcata cgcttcaacg cgccaggcat aacgttttcc ttcgatcaat ggtggtttat
     3421 cggcaccata aaatatactt tgtgtgatga tgttttcttc ctcataaacc atatctaagg
     3481 gagaagtgct ttctaatgcc gattgaatat caattccttc gggataataa accaatttaa
     3541 accgatacat aacagttgat cctagcggaa tgctcatgga aggcgaccaa ctaaatgtta
     3601 ggttttgtgg ttgagtggct ggaaaagttt gttcgcaatt gaaataatcg ggaaccggtg
     3661 cagttggaaa tgaaatggaa aattcgaaac atccattatt tagtactgct tcgggtgata
     3721 gcgcttcgtg ttcggcgctg aaataattat aagcacgcaa acagagttga taatttcctt
     3781 cgggtaatat tccatctata tagcttgccg gatcaacgtt aaccgtttga atgtctccaa
     3841 tatcgttgaa gatatttgca tcaggtcctg taaataccga tgctcccggc attattacta
     3901 aaccgggagc tgtccatggg ttagaaggat cggtaaacac gcgtgcgccg gtgctggtat
     3961 tttctaaact tccggctaaa taaatctgat aagtttcggt tgtactattc gtaattgtta
     4021 ccgttaaaga tgtaggatta ttcacataag cctgataggt agccgaatac ggtggtgcaa
     4081 tatttacatg aacttgtaag ggtagttgtc ccattaatgg cacatgcact aaccataaaa
     4141 tgaaaataaa taggtgtttt ttcatttttt ctgctttgaa tgaaatgcaa atacatatcc
     4201 agccatcatc ctcatttcgg agaatgattg taaggtggaa ctagaatgta aaaagttgat
     4261 atttacttga aattgatgtc cgcctttgag ttgataagaa cccgtgaaat aggcattagc
     4321 aatggatccg gatgcttgct gattgacccg aataaattga tacatcagat taccattaaa
     4381 cttgagttta ttattccgaa aggattttcc tattccaatt aaaggtccta atccaactcg
     4441 atcacctata aatgacattg ttttattata ggtcaatcca cttgtaatgc ttaatttatc
     4501 tcgatttcta aagtaattac tgttcagatt ggcaaataca atctggtttt cactcatgga
     4561 attcgttgaa taggtattta aatcttttaa ttcttgatat ccaactccta aattaattcc
     4621 aataacacgt tgtttattct ggaaatttaa tcgttgattt aattgagcat ttcgacttac
     4681 ttgtgctaca cggaatgtgt cgttaatgct aattaatccg gcttgttgtg ctagtccata
     4741 attggaaaat gaagcatcaa ttccatatac aggttttggt gcaaacgcta tattaaatgc
     4801 agcaatattt cgcatgctgg taaatagttt ttttcgtgat aaattatcct gctctttacc
     4861 aatactagcc tgaattcgca attttccttt cattaattta agtgaaggat ctaccgttag
     4921 tgaacgaaca tcgctctgta aaaacaaagc tccaagtgaa gaatagccag gatcaacttg
     4981 tcgaaaagtg aatttcatac caaatttacg agcttgaaaa ctaaggttcg attgtccggc
     5041 tagcgagagg cgtgtgccta ttcgaggagt tattagctta cttagaaaag gtgaatcact
     5101 ccaatttttt acttctaatt tctcagctag aacattatct gtaatgacac taccggctgc
     5161 ttcaatattc caggtaaaat agcgggtgat agaaaggcgt gaagtcaatc caacaacaaa
     5221 attatccttg ggtgtatatc cttttaaaga agctaa
//
LOCUS       DAOOQW010000164         5236 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_19021_length_5236_cov_5.624590, whole genome shotgun sequence.
ACCESSION   DAOOQW010000164 DAOOQW010000000
VERSION     DAOOQW010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5236
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19021_length_5236_cov_5.624590"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..680)
                     /locus_tag="PK281_08880"
     CDS             complement(<1..680)
                     /locus_tag="PK281_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49501.1"
                     /translation="MTWRFILIRFVLVWAFLGLLNEVYLRSGMHHHDLRMLGATLHLR
                     LDTLQKHNDILYFGESSNATFSANDQDRRSISQLLAERLPDRHVGTIDRGAIHASTYL
                     ALAERINTSSSVKQIVVTLNLRSFGADWIHSGLESEICQDNVMYSNYPALLKRLMVAA
                     GGYDNKETMLRKMYREYQWRTDALPDYVGASNVRSWERKVGEMRQSAKEFNDTASISL
                     AKTYVRSYG"
     gene            complement(680..2080)
                     /locus_tag="PK281_08885"
     CDS             complement(680..2080)
                     /locus_tag="PK281_08885"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HOY49502.1"
                     /translation="MLFNSIHFFFFLPVITALYYLLPEARRWLLILLASCYFYTAFFW
                     PYLFLLFLLIGIDFYAGKLIEKAQGKQRKRYLWISIAANIGLLSIFKYSNFVLFNFNE
                     IALLVGSSFQVKQLNLLLPIGLSFHTFQSLSYVIDVYRGKQKAETDLRVYANYVLFFP
                     QMVAGPIERFSQLGVQFHQQVKFQVMNIVSGLRLVMYGLFVKMCVADPLAEIVTRVYD
                     APSTYSSISVLVALFCFSIQIYADFHGYSTIAIGAARMLGVELMDNFNRPYFASDIHA
                     FWQRWHISLTNWFREYVYYPLGGNRVSKNRWMFNIMLVFILSGFWHGANWTFVVWGAL
                     HGFLYLIWFYVKGKSTAEITDPIFRKVSILATFFLVSLIWVFFRSADFYQVGQIFSAL
                     VHPKNDTQLLLPDAFALIPLVFFIMLEYQRRGERFDLWFSRHKEAVRWGYYAFFLAAI
                     LLLSGIHTHPFIYFQF"
     gene            2279..>3999
                     /gene="pheT"
                     /locus_tag="PK281_08890"
     CDS             2279..>3999
                     /gene="pheT"
                     /locus_tag="PK281_08890"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013871527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOY49503.1"
                     /translation="MKISYNWLQRYISPVPSIDETSRLLTASGLEVEGIEHFESHQGG
                     LRGLVVGEVKTCEQHPNADRLRVTTVDIGQEELLHIVCGAPNVASGQKVIVATIGTTL
                     YPVSGESFVIKESKIRGELSQGMLCAEDEIGLGESHAGILVLPAETPIGKPVSELFNV
                     TEDQVLEIGLTPNRTDAMSHTGVARDLVATLYNDDTYSFQFPKSTIKLNAGDCPVKVQ
                     IETPDALIRYAGICIDGVQVKESPVWLQHALKAIGLKPINNIVDATNFVMHELGQPLH
                     AFDRSSLAGDKILVKTCAEGTSFTTLDGQERKLGANDLMICDAEKPLCIAGVFGGLNS
                     GVKDSTTKLFIESATFHPVWVRKTSRALAIKTDSSFRFERGTDPEMPVVALERVAELI
                     LEIAGGNLSGGLFDWYPNPVAWTQIDLLWSSMNRLIGEVVPQETAKLILQKLGIQIES
                     ENSDGLQLRIPPFKVDVKNSADVTEEVLRIYGYNTIEIPTQVRASISIQEKPDVWKLK
                     NHLSDHLCSIGFTEILNNSLTAASTASYFPESTPVNILNPLSSELSIMRQSLVFGVCE
                     TIAWNKNR"
     assembly_gap    4000..4099
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4100..4480
                     /locus_tag="PK281_08895"
     CDS             <4100..4480
                     /locus_tag="PK281_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013752385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOY49504.1"
                     /translation="YGINEAVWVAVCNWDQLLRQYKKYQLNYQDVPRFPSVRRDLSML
                     IDASTQFDKIVTIARGTERKLLRDVSLFDVYQGDKLPAGKKSYAVSFTFLDEEQTLTD
                     KQIDKTMERLIKAYQEQIGAELRG"
     gene            complement(4483..>5236)
                     /locus_tag="PK281_08900"
     CDS             complement(4483..>5236)
                     /locus_tag="PK281_08900"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOY49505.1"
                     /translation="IVSPAGVAWRTSWETDSTINLWSSDNSHPSLAGSYLTACVFYST
                     ILRKSPVGLSYHAGLADSIASFLQQVAHQTVLDSLDTWNIGKWDVKSEFTFVLDGENV
                     SFVNQSDNATTYSWDFGDGTALSSETSPNHTFESTFGNSFTVQLIAQNACESDTSMIT
                     FEIIPTGIPTGLVESEWRVFPNPASKQVMIQASKSTQFTIEIYNSEGILMRRFQEEGS
                     LIPLSVETYSPGIYQVVCSSGKEQRIFRMLKN"
BASE COUNT         1647 a         1064 c         1051 g         1374 t
ORIGIN      
        1 ccatagctgc gcacataggt tttagccaaa gagattgaag cggtgtcgtt gaattctttg
       61 gcagattgac gcatttcacc cactttcctt tcccaggatc gaacattcga ggctcctaca
      121 taatcgggca atgcatcggt tcgccactgg tactcacggt acattttacg cagcatcgtt
      181 tctttgttgt cgtatccgcc agccgctacc atcaagcgtt ttaacaaagc tggatagtta
      241 gaatacatca cattatcctg acatatttcc gactccaaac ctgagtgaat ccaatcggct
      301 ccaaacgaac gaagattaag cgttacaaca atctgcttga ccgaagaact ggtattaatt
      361 cgttcagcta aagccaagta ggttgaagca tgaatcgcac ctcgatcaat tgttccaaca
      421 tgtcgatcgg gcaagcgttc ggctaatagt tgactgatag atcttcggtc ttgatcattt
      481 gccgaaaaag tagcattaga cgactctcca aaataaagta tgtcgttatg tttttgtaag
      541 gtatctaagc gaagatgtaa tgtagcacct aacattctta agtcgtgatg atgcattcct
      601 gaacgcagat atacttcgtt gagcaatccg aggaaagccc ataccagcac aaatcgtatc
      661 aagataaatc gccaggtcat tagaactgaa aatagataaa tggatgggtg tgaattccgc
      721 tcaacaataa gatagcagcc aagaagaatg cataatagcc ccaacgaaca gcctctttat
      781 gccgactaaa ccacaagtcg aaccgctcac ctcgacgttg atattcgagc ataatgaaga
      841 acaccaatgg tatcaacgca aaagcatctg gcaataaaag ttgagtgtca tttttagggt
      901 gaacgagagc agagaatatt tgtccgactt ggtaaaaatc agcacttcta aaaaacaccc
      961 aaatgagact gaccaagaag aatgttgcta ggatagatac tttcctgaaa atcggatcgg
     1021 tgatttcggc tgtggactta ccttttacat aaaaccatat caggtaaaga aacccgtgta
     1081 aagctcccca aacgacaaag gtccaattgg caccatgcca aaatccgctg agaatgaaaa
     1141 cgagcataat attgaacatc caacgatttt tactcacgcg atttccaccc aaaggatagt
     1201 acacatattc gcgaaaccaa ttggttaagg aaatgtgcca acgttgccaa aatgcatgga
     1261 tatcactggc aaaatagggg cgattaaaat tatccatcaa ttccacacct agcatgcggg
     1321 ctgcaccaat ggcgatggtc gaataaccat gaaaatcagc ataaatctgt atcgaaaaac
     1381 aaaacaaagc aaccagcacc gatatagaac tataagtgga tggagcatcg tacacccgag
     1441 ttacaatttc ggcaagtgga tcggccacac acattttcac aaataatcca tacatcacca
     1501 aacgtaaacc gctaacgatg ttcatcacct gaaacttcac ctgttgatga aactgcacgc
     1561 ctaactgaga aaaacgttca attgggccag ctaccatttg tgggaagaaa agcacatagt
     1621 tagcgtaaac acgtaaatcg gtttcggctt tctgcttccc gcgatataca tcaatcacat
     1681 aactaagcga ttgaaacgtg tgaaaggaaa gtccaatagg tagcaacaaa ttcaactgct
     1741 tcacctgaaa agaacttcct acgagtaaag cgatttcgtt gaaattgaac aggacgaaat
     1801 tgctgtattt gaaaatggat agcagtccga tattggcggc aatgctaatc cacaaatacc
     1861 gtttccgctg cttgccttgc gctttttcga taagcttccc agcataaaaa tcaattccaa
     1921 ttaagaggaa taacaaaaac aaatacggcc agaaaaatgc tgtgtagaaa taacaactcg
     1981 ccagtaatat caacagccag cgtcgagctt ctggaagcaa ataatacaat gccgtgatga
     2041 caggcagaaa gaagaagaaa tgaatggaat tgaaaagcat ctcagcgaat cggccttcaa
     2101 ataggaatgc aaactaaaaa caaaatccca gtatttacag gaaaatatga gataattcga
     2161 gcatgtgtaa gctgttggcg gattttcgat tcttcctgct aatcaattaa aaataaacgc
     2221 taaactgaag atagcttaac gctttcctcg tattttagcg cccccgaata agcaaccaat
     2281 gaagatttcc tacaactggc ttcaacgcta catttctcct gttccatcaa tcgacgaaac
     2341 atctcgtttg ctcaccgcat cgggcttgga agtggaagga attgagcatt tcgaaagtca
     2401 tcaaggaggt ttgagaggac ttgttgttgg tgaagtaaaa acatgtgagc aacatccaaa
     2461 tgccgatcgc ttacgtgtta ctaccgtgga tattggacaa gaagaattgt tgcatatcgt
     2521 atgtggagcg cccaacgtag caagtggaca aaaagtgatt gttgccacca ttggaactac
     2581 gctttatccg gtttcgggag aatcgttcgt catcaaagaa agtaaaatca gaggcgaact
     2641 ttctcaagga atgctttgcg ctgaagatga aattggttta ggtgaaagtc atgctggtat
     2701 tttagtactt ccagctgaaa cacccattgg caaacctgtt tcagagttgt ttaatgtgac
     2761 agaagatcaa gtgctcgaga ttggactaac tcccaatcgt accgatgcta tgtcgcatac
     2821 aggtgttgcg cgcgatttag ttgcgacact ttataatgat gatacttatt cgtttcaatt
     2881 tccgaaaagc actatcaagc tgaatgctgg agattgtccg gttaaggttc aaatcgaaac
     2941 tcccgatgca ctcattcgat atgcaggaat ttgcatcgat ggtgttcagg tgaaagaaag
     3001 tccggtatgg ttgcaacatg cgcttaaggc tattggtttg aagcctatca acaacatagt
     3061 tgacgcgaca aatttcgtta tgcatgagtt gggacaacca ttacatgcat tcgatcgcag
     3121 ctcgttagca ggcgataaaa ttttagttaa aacttgtgca gaaggaactt catttaccac
     3181 actcgatggt caagaacgta agttaggagc gaacgatttg atgatttgtg atgcagaaaa
     3241 accactgtgt attgcaggtg tatttggcgg tttgaattcg ggtgtgaaag attctacaac
     3301 caaattgttc attgaatcgg caacgtttca tccagtttgg gttcgtaaaa cttcgcgtgc
     3361 tttagctata aaaaccgatt cttcctttcg attcgaacgc ggaacagatc ctgaaatgcc
     3421 tgttgttgcg cttgaacgtg tggcagaact gattttagaa atcgctggag gaaatctttc
     3481 tggtggttta ttcgactggt atcccaatcc ggtagcttgg acgcaaatag atttactttg
     3541 gtcaagcatg aatcgcttga ttggtgaagt cgttcctcaa gaaactgcca aactcatcct
     3601 ccaaaagtta ggtattcaaa ttgaatcgga gaattcggat gggttacaat tgcgtattcc
     3661 tccattcaag gttgatgtaa aaaactccgc cgatgttacc gaagaggttt tgcggattta
     3721 tggatacaat accattgaaa ttcctactca agttcgggct tctatttcga ttcaggaaaa
     3781 accggatgtg tggaaactaa aaaatcattt aagcgatcat ttatgcagca tcggttttac
     3841 cgaaatcttg aacaactcgc ttactgccgc cagtacagct tcttatttcc ctgaatcaac
     3901 accagttaac atactcaatc cattaagttc cgaattgagt atcatgcgtc aaagtttagt
     3961 ttttggcgtg tgtgaaacga tagcgtggaa taagaaccgn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnt atggcattaa tgaagcggtt tgggtagctg tatgtaattg
     4141 ggatcaactc cttcgtcaat acaaaaaata ccagctaaac tatcaggacg ttccacgctt
     4201 tccttcggta cgtcgcgact tatctatgct aattgatgca tcaactcaat tcgataagat
     4261 tgtgaccatt gctcgtggaa ccgaacgtaa attactacgt gatgtatcct tgttcgatgt
     4321 gtatcaagga gataaacttc ctgcaggaaa gaaatcgtat gctgtttcgt tcaccttctt
     4381 agatgaagag caaaccttga ccgataagca aattgataaa acaatggaac gcctgataaa
     4441 agcatatcag gaacaaattg gtgctgagtt aagaggataa aatcagtttt tcagcatacg
     4501 gaaaattcgc tgttcctttc cagaactgca taccacttga taaatccctg gtgaatacgt
     4561 ttcaacactg agcggaatca aacttccttc ttcctgaaat ctacgcatca atattccttc
     4621 ggaattataa atttcgatgg taaactgggt cgatttagaa gcctgaatca ttacttgctt
     4681 agaagcagga tttggaaaaa cacgccattc cgattcaacc aatccagttg gaattccagt
     4741 tggaataatt tcaaacgtta tcattgaagt atcggattca caggcatttt gagcaattag
     4801 ctgaacagta aaagagttac caaaagttga ttcaaacgta tgattaggcg atgtttccga
     4861 gctcaaagct gttccatctc caaaatccca gctataagtt gtggcattat ccgattgatt
     4921 tacaaaactc acattttcac catccagcac aaatgtgaat tctgatttaa catcccattt
     4981 cccaatattc catgtatcca agctatctag aactgtttga tgagcaactt gctgcaaaaa
     5041 agaggcaatc gaatcggcta atccagcatg ataacttaaa ccaacaggtg attttcgaag
     5101 aatggtacta taaaaaacac aagctgttaa ataagatcca gctaaactag gatgggaatt
     5161 atcggatgac caaagattaa tggttgaatc ggtttcccaa ctagttctcc aagcaacacc
     5221 agcaggtgag acaatc
//
LOCUS       DAOOQW010000165         5222 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_19090_length_5222_cov_5.171279, whole genome shotgun sequence.
ACCESSION   DAOOQW010000165 DAOOQW010000000
VERSION     DAOOQW010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5222
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19090_length_5222_cov_5.171279"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..838)
                     /locus_tag="PK281_08905"
     CDS             complement(<1..838)
                     /locus_tag="PK281_08905"
                     /inference="COORDINATES: protein motif:HMM:NF017072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HOY49506.1"
                     /translation="MTDDRNPLLRFAAGNVMLPQEEMLEVGRRSSELQIGIPKETSFQ
                     ERRVSLVPAAVAVLVNNGHQITVESGAGTNANYQDKDYSDAGATIAYGPEQVYKCDIV
                     LKVAPPTFDEIEMMNQKQKLISALQLTVQQKNFFNHLISKKITGLAFDYIKDRSGIYP
                     VVRSMSEIAGNTSILIAAEYLSTTNNGIGMMLGGISGVPPTEVVIIGSGTVSEFAARA
                     AIGLGAHVKVFDNSLYRLRRIQNDLGQRLFTSIIEPRQLEAALATADVVIGALRAKEG
                     RTP"
     gene            complement(852..1298)
                     /gene="tsaE"
                     /locus_tag="PK281_08910"
     CDS             complement(852..1298)
                     /gene="tsaE"
                     /locus_tag="PK281_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015480230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOY49507.1"
                     /translation="MNITFECKTLEELAEGAEILTSNFAHQHVFAFYGEMGAGKTTFI
                     RAICQELGTKDSVSSPTYGLVNEYALPSGKSIYHFDFYRIKSIDEAVEIGLDEYLHSG
                     AWCFIEWPENIESLLPANYVKVTLTEHREVRSIHMQNHTGNVSLGN"
     gene            1399..2076
                     /locus_tag="PK281_08915"
     CDS             1399..2076
                     /locus_tag="PK281_08915"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOY49508.1"
                     /translation="MNKTDLGILFDCDGTLTNSLDQALESFVITFQELGLTHITEEEI
                     LKLFGISADKILRHFVGNDQEKSALAFNIFLSTQKKLALKTKLHIGISVVIDAFVQMK
                     IPMGIVTGRHAADLDILLSPHGLAEFFPVKICDNMVAKPKPDPQGIWMACQYLDKKPE
                     RMLYIGDSVVDIQAAHASGCTSVAALWDSHTNESALLAENPAYCIRHPEELLEVWADF
                     KQKSRSN"
     gene            complement(2110..3663)
                     /locus_tag="PK281_08920"
     CDS             complement(2110..3663)
                     /locus_tag="PK281_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014034401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PglZ domain-containing protein"
                     /protein_id="HOY49509.1"
                     /translation="MDKINILWADDEIELLKPHILFLREKGYDVQTANCGDDALDLFK
                     EQVFDIVFLDENMPGLSGLETLVKIKQLKNDVPVVMITKSEEEHIMEDAIGSQISDYL
                     IKPVNPNQILLSLKKNLENKRLIREKTSSAYQQEFRSIGMTLSDRLSIDEWKEVYQKL
                     VFWELELNKSKEEGMEEILRMQKTEANTQFSKYVINNYQGWVNGKDPNPPLMSHNIFK
                     QRVVPEIDKNESTFFVLIDNMRYDQWKVMQPILQDYFKVDTEEMFCAILPTATQYARN
                     AMFAGLMPSEIEKRYPNHWLNDEDEGGKNLHEEFFMNEQLKRLGKDIKTSYTKVTNNQ
                     AGKKLAENIPNLMGNKFNAIVYNFVDMLSHARTEMEVIRELADDEKAYRSITVSWLEH
                     SPLLDILRQIADKKARLILTTDHGTIRVTNPSKIIGDRNTNTNLRYKQGKNLNYEKKD
                     VYEVKNPLDIFLPRQHVSTAFVFAKEDLFFAYPNNYNYFVNYYRNTFQHGGISLEEML
                     IPFITLSNK"
     gene            3761..4975
                     /locus_tag="PK281_08925"
     CDS             3761..4975
                     /locus_tag="PK281_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010521930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOY49510.1"
                     /translation="MNDPVYGFISIPDELLFDLIEHPWFQRLRRIRQLGLTDYVYPGA
                     QHTRFHHALGAMHLMGLAIENLRYKGHEITELEALSAQAAILLHDIGHGPFSHALESS
                     IVPGLHHEQLSLLFMQELNRQFGGKLGMTIEIFTGKYHKHFLHQLVSSQLDVDRLDYL
                     NRDSFYTGVAEGTVGSDRIIKMMEVVNGELVLEEKGIYSIEKFLLARRLMYWQVYLHK
                     TVLSAENLLSNMLRRAKYLVQKGEVVFATKSLMYFLEHTISSPSESIDEELLERFAHL
                     DDYDIFASAKEWMTHSDKILSELSTRLIERKLLKIRLDVQPFDAAEVDALSQKWQSKG
                     FSKVEADYFVFTGGITNSAYKDGEEKINILLKTGKVLEIREASDQLRMSVLSESTRKY
                     FLCYPKELDTDH"
     gene            4982..>5222
                     /locus_tag="PK281_08930"
     CDS             4982..>5222
                     /locus_tag="PK281_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008464038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LpxD N-terminal domain-containing protein"
                     /protein_id="HOY49511.1"
                     /translation="MEFTAQQIADLLQGRVEGDPEVRVNRLSKIEEGEPGSLTFLANP
                     KYEEFIYSTKASLVIVNDSFVPDRAIGTNLVKVKDA"
BASE COUNT         1559 a          960 c         1024 g         1679 t
ORIGIN      
        1 tgggtgttct accttctttt gcacgtaatg ctccaataac aacatcggca gtagcaagtg
       61 cagcttctaa ttgacgcggt tcgataatcg aagtaaataa tcgctgtcct aaatcgtttt
      121 gaatgcgacg taatcggtac aaagaattat caaacacttt cacatgagca cctaatccaa
      181 tagcagctcg tgcggcaaat tcacttacag ttcctgatcc gataatgact acttctgttg
      241 gaggaacacc tgaaatgcct cctagcatca taccaattcc attattggtt gtacttaaat
      301 attcggctgc gatgagaata gaggtatttc cagcaatctc gctcatagaa cgaacaactg
      361 gataaattcc tgaacggtcc ttgatgtaat cgaaagctaa accagtaatt ttttttgaaa
      421 tcaggtggtt gaagaaattc ttttgttgaa ccgttaattg cagagccgaa atcagcttct
      481 gtttttggtt catcatttct atttcatcga aagtaggagg agcaactttt aaaacgatat
      541 cacatttata aacctgttct ggtccatagg cgatagtagc accagcatca ctgtaatctt
      601 tgtcctgata gttggcattt gtaccagcac ctgattcaac ggtaatttga tgtccattat
      661 taactaatac tgcaactgca gctggaacga gagaaacgcg tctttcttgg aaagaagttt
      721 ctttcgggat tccaatttgt agttcgctag aacgccgccc aacttcgagc atttcttctt
      781 gaggaagcat gacattacct gctgcaaatc tcagtaatgg gttacggtcg tcggtcataa
      841 ttagtcttca attaattgcc taaagataca tttcctgtgt ggttttgcat atgaatgctg
      901 cgaacttccc ggtgttcagt taatgtaact ttcacataat tagctggcaa gagtgattca
      961 atattttcgg gccattcgat aaaacaccaa gcacctgaat ggagatattc atccagtcca
     1021 atttcaacag cttcgtcgat agatttaata cgataaaaat caaaatgata aattgatttt
     1081 cctgaaggaa gcgcatattc gttaactaaa ccataagtag ggctactcac agaatctttc
     1141 gttcctagtt cttggcaaat agcacgaata aatgttgttt ttcctgctcc catttcgcca
     1201 taaaaagcga aaacgtgttg atgtgcaaaa ttactagtaa ggatttccgc cccttcggct
     1261 agttcttcca gcgttttaca ttcaaaagtg atattcatgg agcggaaatc ggtatggcaa
     1321 agatacttgc tggattgtgt ttttgggatt ctcttcaata aagaatgtgg atattcgttt
     1381 ttttaattaa aattcaacat gaataaaacc gatttgggga ttttatttga ttgtgatggc
     1441 acattaacca actcgcttga tcaagcatta gaatcatttg taattacctt tcaagaacta
     1501 ggattaacgc acattacaga agaagagatt ctaaaactat ttggaatttc tgctgataaa
     1561 attctcagac attttgtagg aaatgatcaa gaaaaatcag cattagcttt taatatattt
     1621 ttatcgactc agaagaaact tgcgttaaaa acaaaattac acataggaat atcagtagtc
     1681 attgatgcat ttgttcaaat gaaaatacca atgggaatag taacaggacg acacgctgct
     1741 gatttagata ttttgctttc accccatgga ttagctgaat tttttccagt aaaaatttgc
     1801 gataacatgg ttgctaagcc caaacctgat cctcaaggaa tttggatggc ttgccaatat
     1861 ttagataaaa agcctgaacg tatgttatac ataggagatt cggttgtgga tattcaagct
     1921 gctcatgctt caggttgcac ttctgttgcc gcattgtggg attcacatac gaatgaatca
     1981 gcgttattag ccgagaatcc agcgtattgc attcgtcacc cggaagaact attagaagta
     2041 tgggctgatt tcaaacaaaa atcccggtca aactgagagt ctaaccggga ttttaataaa
     2101 tcaggattat tacttattac tgagtgtaat aaatggaatg agcatttctt cgagtgaaat
     2161 tccgccgtgt tggaatgtgt tgcggtagta attcacgaaa tagttgtagt tattcggata
     2221 cgcaaagaat aaatcctctt tggcaaaaac gaaagctgta ctcacatgtt gtcgtggcaa
     2281 gaaaatgtcg agtggatttt tcacttcata cacatctttc ttttcgtaat tcaagttttt
     2341 accttgtttg tagcgaagat tcgtattggt gtttcgatca ccaataatct tgcttggatt
     2401 tgtaactcga attgtaccgt gatcggttgt tagaatgagt cttgcttttt tatccgctat
     2461 ttgtcgaagg atatcgagta ggggagaatg ttctaaccaa gaaacggtga tagagcgata
     2521 tgctttttca tcatcggcta attcacgaat cacttccatt tcggtacgtg catgtgaaag
     2581 catatccacg aaattgtaca caatagcatt gaacttattt cccattaaat tcggaatatt
     2641 ctctgctaac tttttaccag cttggttatt ggtgactttt gtataggacg ttttaatgtc
     2701 ttttccgagg cgtttaagtt gttcattcat gaagaattcc tcatgaagat tttttccacc
     2761 ttcatcttca tcgtttaacc aatgattagg ataacgcttt tcaatttcag aaggcattaa
     2821 accagcaaac atggcatttc tcgcatattg agttgctgta ggtaaaatgg cacagaacat
     2881 ttcttccgta tctactttaa agtaatcttg aagtatgggc tgcattacct tccattgatc
     2941 ataacgcata ttatcaatca atacgaagaa agtactttcg ttcttatcga tttcaggtac
     3001 tacacgttgc ttgaaaatat tgtgtgacat caaaggtgga tttggatctt ttccattcac
     3061 ccagccttga taattgttga ttacatattt agagaattgc gtattcgctt cggttttctg
     3121 catacggagg atttcttcca taccttcttc cttgctttta tttaattcca attcccagaa
     3181 aaccaatttc tgataaacct ctttccattc gtctatcgaa agtcggtcac tcaatgtcat
     3241 tccaatactt ctaaattcct gttgataagc acttgaagtt ttttcacgta ttaaacgttt
     3301 gttctctaag ttctttttaa gggaaagaag tatctgattt ggattgacag gtttaatcaa
     3361 gtaatcagat atctgagaac caatggcgtc ttccatgata tgctcctcct cacttttagt
     3421 aatcattaca accggaacgt cgttcttaag ttgtttgatt tttacaagcg tttccaatcc
     3481 ggaaagtcca ggcatatttt catccaagaa aacaatatcg aacacttgtt ctttgaataa
     3541 atcgagtgca tcatcaccac aattggctgt ttgcacatcg tatccttttt cccgtaagaa
     3601 taggatatgt ggtttcaata actctatttc gtcgtctgcc caaaggatgt tgatcttgtc
     3661 catgtatctt tgctaaatga aggtgctcaa aagtacctgt tgaaacacca taaatccgtt
     3721 aatattgccg gtgaacctta cgcgaaacaa aaaaaaaatt gtgaatgatc ctgtatatgg
     3781 attcatttca attcccgacg agttattatt tgatctgatt gaacatccgt ggtttcagcg
     3841 tttacgccga attcgccagt taggattaac cgattatgtt tatccaggcg cgcaacatac
     3901 acgtttccat catgcactag gtgcaatgca tttgatgggg cttgccatcg aaaacctacg
     3961 ttataaagga catgaaataa cagaactaga agcattatca gcacaagcgg ctattttact
     4021 tcatgatatc ggacatgggc ctttttctca tgcccttgaa agttctattg ttcctggttt
     4081 gcatcacgaa cagttgtcgt tgctatttat gcaggagctt aatcgacagt ttggtggaaa
     4141 actgggtatg acaatagaga ttttcactgg aaaatatcat aaacattttc tgcatcagtt
     4201 ggtttcaagt cagttagatg tggatcgctt agattatttg aatcgtgata gtttttacac
     4261 aggtgtagct gaaggtactg ttggttcaga tcgaatcatc aagatgatgg aagtggtgaa
     4321 tggtgagttg gttttggaag agaagggaat ttatagtatc gaaaaattcc ttttagcaag
     4381 acgtttaatg tattggcaag tatatctgca taagaccgtt ttatcggctg agaacttatt
     4441 gtccaatatg ctacgtcgtg ctaaatacct agtccaaaaa ggagaagtag tatttgcaac
     4501 taaaagcttg atgtattttt tagagcatac catttcttca ccttcagaat caattgatga
     4561 agagctattg gaacgtttcg cccacttgga tgattatgat atatttgcat ccgcaaaaga
     4621 atggatgaca cattctgata aaatcttatc ggagctgagt accagactga ttgaacgaaa
     4681 gctgttaaaa attcgcttgg atgttcaacc ttttgatgca gcagaagtcg atgcgctaag
     4741 ccaaaaatgg caaagcaaag gtttttcgaa agtagaagca gattattttg tttttacggg
     4801 tggcattaca aacagtgcat acaaagatgg agaagagaaa atcaacattc tactcaaaac
     4861 aggtaaggtt ctcgagattc gagaagcttc agatcaattg cgaatgtcgg ttttgtcaga
     4921 atcaacccgg aaatacttct tgtgttatcc aaaagagtta gacacggatc attagaaacg
     4981 aatggaattt acagcacaac agattgccga tctgcttcag ggcagagtag agggtgaccc
     5041 cgaggtgagg gttaatcgac tttcaaaaat tgaagagggc gaacctggtt ctctgacatt
     5101 cttggccaat cctaaatatg aggagtttat ctattcaacc aaagcatcat tggttattgt
     5161 aaacgattcg tttgtccctg atcgggctat aggaaccaat ttagtgaagg ttaaagacgc
     5221 gt
//
LOCUS       DAOOQW010000166         5219 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_19106_length_5219_cov_5.109992, whole genome shotgun sequence.
ACCESSION   DAOOQW010000166 DAOOQW010000000
VERSION     DAOOQW010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5219
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19106_length_5219_cov_5.109992"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1237
                     /locus_tag="PK281_08935"
     CDS             <1..1237
                     /locus_tag="PK281_08935"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HOY49512.1"
                     /translation="PVHVDRYTGNSLGFGLLTVDIPGFFDREGIYIDTHTGYGYSDEA
                     ERYVLFQQTVLKFLLESPEKPDIIHCHDHHTGLIPFMIKHCPEYRALSEIPTMFTIHN
                     GQYHGAFSWSRSWILPWYDANANGLLDWDHNINPLACAIKCCWKFSTVSGGYLNELRY
                     AANGLESLINMEFHKAYGIVNGIDNQVWDPKNDPLLAYPLVENIDEYKAQNKQVIAER
                     FAIQADLPLITFIGRLVGEKGADLIPGAIMRFLEQGGQASFVVLGTGDPALRDVFMQM
                     RERLWSYFDTSIEYNEQLSHQLYAGSDFLMMPSRVEPCGLNQLYSFRYGTIPIVRSIG
                     GLAETVIDVTQWEGNGLRFDDLSEDALCRSFHRAAELFQRKDILIPLRERIMKLDFSW
                     EKAADTYISIYKTLQPSFA"
     gene            1234..2520
                     /locus_tag="PK281_08940"
     CDS             1234..2520
                     /locus_tag="PK281_08940"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015480935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HOY49513.1"
                     /translation="MKGTIKMICLILGGGQGSRLYPLTSSRSKPAVPIGGKYRLIDIP
                     ISNCINSGVRRMFVLTQFNSASLNRHIKNTYHFDNFSAGFVDILAAEQTPNSDKWFQG
                     TADAVRQSMHHLTGHEFDYVLILSGDQLYQMDFKLMAEFHLDQNADLTIATIPVDKQD
                     APEFGIMKVNKHNVIETFIEKPKPDVLSNWTSPVPAPLKKKGREYLASMGIYIFRKDA
                     LKRLFDENPDATDFGKGIIPAAINSGYNVSSYQFDHYWTDIGNIRSFYEANLELCDNL
                     PKFNLFDNHKRIYSRARALAPSKFFGTRLNKSIVAEGCIVHAKLIDYSVIGVRSRIGE
                     KTVVKNAIIMGNDYYQSLEEIVLQKDEIPMGIGKSCHIEQAIIDKDCRIGNNVIIKGD
                     NSLPNSETEHYCIVEGIVVLKKKAIIPDGTRIGKIN"
     gene            2539..2766
                     /locus_tag="PK281_08945"
     CDS             2539..2766
                     /locus_tag="PK281_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49514.1"
                     /translation="MNFSSLNLKLSSLGIVFLLSLSTTFLNAQTDQIGRVEVSLEDEW
                     GDFFVIPMKENGVILGSFGTKATFNLRLHPL"
     gene            2795..3883
                     /locus_tag="PK281_08950"
     CDS             2795..3883
                     /locus_tag="PK281_08950"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HOY49515.1"
                     /translation="MKNLIVLIILSLTSSLLFSQTNEELPFSTINFVRTGNYFGSACR
                     SDITLPNQREFNLSHKSIVKYKVYSEGEINITLNVFCPGANGGPSKSQSSQISINVKR
                     GEEYYVLYSKGALEQVQKADVEEYLIKIDNIQKHQENIEFPIIKSSINDIAKKDGKGQ
                     GTCFLISSDGYLVTNYHCIENAKEITIKGIGSDYTTKYGVTIVATDPSNDLALLKISN
                     KNLKFSIPPFAIRSSGVAQAEKIYALGFPQTKSMGEELKITEGIISSKSGAQGDISKF
                     QISAAVNPGNSGGPLIDEEGNLVGVIYAKSKVAESAGYAIKASYLETFLKNIDGFEFP
                     NLTNLIKEKPLTEKVEILKDYIFILECN"
     gene            4100..4657
                     /locus_tag="PK281_08955"
     CDS             4100..4657
                     /locus_tag="PK281_08955"
                     /inference="COORDINATES: protein motif:HMM:NF026080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4468 domain-containing protein"
                     /protein_id="HOY49516.1"
                     /translation="MKLAFLGLTLQGGMLLAQTPAAPKMPVDEISKLITYTEVVDEPG
                     MNKDTLYNRGLRWFKSYFKNPNKAIKLADAEGRTIDGGYTFTIQRPDPASKKTPAPMV
                     DAGLVNFKMKLFFKDAKFKYEITNISWQQTSYYPIERWMDKEAKNYDPNYALYLQQTD
                     QYMKTLIKSLETFIETDPLKKKDDW"
     gene            complement(4808..>5219)
                     /locus_tag="PK281_08960"
     CDS             complement(4808..>5219)
                     /locus_tag="PK281_08960"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY49517.1"
                     /translation="EVFKVSDYIANPGMVKFILEATDFPNGHIVEAGLDRFYVTDSFT
                     VNIANPLLEDAELVSYPNPSTGLVSVKWHVSKTVKQAKLEFCDLQGRVIQEQQLVSDS
                     GLIKLGLNVNSGIYLMRLIADGRIVRSEKIVITQ"
BASE COUNT         1691 a          973 c          986 g         1569 t
ORIGIN      
        1 tccggttcat gtagatcgct atactggaaa ttctcttgga tttggcttgc taacagttga
       61 tattcctggc tttttcgatc gggaaggaat atacatcgac acgcatacag gctatggata
      121 cagcgatgaa gccgaacgct atgtgctctt tcaacaaact gttttaaagt tcttactcga
      181 atcgcccgaa aagcccgata tcattcattg tcatgatcat cataccggat taattccgtt
      241 catgattaaa cactgtcctg aatatcgtgc tttgtccgaa atacctacca tgtttaccat
      301 ccacaacgga cagtatcatg gtgcatttag ctggagcaga agttggattt taccttggta
      361 tgatgccaat gctaacggtt tgctcgattg ggatcataac atcaatcctt tagcatgtgc
      421 gataaaatgc tgctggaaat tcagtaccgt ttcgggtgga tatttgaatg aattacgcta
      481 tgcagcaaat ggcctagaaa gcttgataaa catggaattc cataaagcgt atggaattgt
      541 aaatggaatt gataatcaag tttgggatcc gaaaaatgat ccactcctag cctatccttt
      601 agttgagaat attgatgaat ataaagcaca aaacaaacaa gtaatcgccg aacgttttgc
      661 cattcaagcc gatttgcctc tcatcacgtt tattggaaga ttagttggtg aaaaaggtgc
      721 cgatttgatt cccggtgcta ttatgcggtt tctagaacaa ggcggtcaag cttcttttgt
      781 tgtccttgga actggcgatc ctgctctgag agatgtgttc atgcaaatgc gcgaacgctt
      841 gtggagttat tttgatacat ccatcgaata caacgaacaa ctttctcatc aactatatgc
      901 gggtagcgat tttctgatga tgccttctcg tgtggaacca tgtggattaa accaattgta
      961 ttcatttcga tacggaacga ttcctatcgt tcgaagcatt ggtggtttag ccgaaacggt
     1021 aattgatgtc acacagtggg aaggaaacgg acttcgtttc gatgatttaa gtgaagatgc
     1081 actttgtcgc tcatttcatc gtgcagccga actttttcaa cggaaagaca ttttaattcc
     1141 gcttcgggaa agaatcatga aactcgactt tagttgggaa aaagcagccg atacgtacat
     1201 ttcaatttat aaaacacttc aaccttcatt cgcatgaaag gaactattaa aatgatttgc
     1261 ctgattttgg gcggtggaca aggcagccga ctttatccac ttacgagtag tcgctctaaa
     1321 cctgcagttc ccattggagg aaaataccgc ttaattgata ttcccatttc caattgtatt
     1381 aattcgggag tacgacgcat gtttgtgttg acgcaattca attcagcctc gctaaatcgt
     1441 cacataaaaa acacctatca cttcgataat ttcagtgctg gatttgtgga tattctagcc
     1501 gcagaacaaa ctcccaacag tgataaatgg tttcagggta cagccgatgc agttcgtcaa
     1561 agtatgcatc acttaactgg acacgaattc gattacgtgt tgattttgag tggtgatcaa
     1621 ttgtatcaaa tggatttcaa gttaatggct gaattccatt tagatcaaaa tgccgatttg
     1681 acgattgcta ccattccggt tgataaacaa gatgctccgg agtttgggat tatgaaggtg
     1741 aataaacaca atgtaattga gacgttcatc gaaaaaccta agccggatgt gttgtccaat
     1801 tggacttcac ccgtacctgc tccacttaag aaaaaaggtc gggaatactt ggcttccatg
     1861 gggatttaca ttttccgaaa agatgcgttg aagcgtttat ttgatgaaaa tcctgatgcc
     1921 actgattttg gaaaaggtat tattccagca gctattaatt cgggctacaa tgtgtcttcg
     1981 taccagtttg atcactattg gacagatatc ggaaacatcc gttcattcta tgaggcaaac
     2041 ttggaacttt gcgacaatct gcccaaattc aacctgttcg ataatcataa acgtatttac
     2101 agtcgagcac gtgcattagc tccatctaag ttttttggaa ctcgtttgaa taaatcaatt
     2161 gtggccgaag gatgtatcgt tcatgcaaaa ctgattgatt attctgttat tggagttcgt
     2221 tcccgaattg gtgaaaaaac cgtcgtgaaa aatgccatca tcatggggaa tgattattat
     2281 caatcgttgg aggaaattgt cttacagaaa gatgaaatcc cgatgggaat tggtaaatcg
     2341 tgtcacatcg aacaagccat catcgacaaa gattgccgaa ttggaaataa tgtaattatt
     2401 aaaggcgata attctcttcc taacagcgaa acggagcatt attgtatcgt tgaaggtatc
     2461 gtcgttttga aaaagaaagc aattatcccc gatggtactc gaatcggcaa aatcaattaa
     2521 aagaaataaa tctactaaat gaatttttct tctttgaacc taaagctaag ttctttaggc
     2581 attgtatttt tattgtcatt atcgaccact tttctaaatg ctcaaaccga ccaaattggg
     2641 agagttgaag tttccctaga ggatgaatgg ggtgatttct ttgtcatacc gatgaaagaa
     2701 aacggtgtaa ttctcggttc atttggcacg aaagctacgt ttaacttacg cttgcaccca
     2761 ttatgaaata tttacacttt caaaaaattt aactatgaaa aatttaattg ttttaatcat
     2821 tcttagtctt acttctagtc tattattttc acagacaaat gaagagttac cattttcaac
     2881 aataaacttt gttaggactg gaaattattt tggatctgct tgtaggagcg acatcacctt
     2941 acctaatcaa agggaattta atttgagtca taaatctata gttaagtata aggtttattc
     3001 tgaaggcgag attaatatta cacttaacgt cttttgtcca ggtgcaaatg gaggtcctag
     3061 taaatctcaa tcctcccaaa tttctataaa cgtaaagaga ggcgaagaat attatgtatt
     3121 atatagtaag ggggcgcttg aacaagttca aaaggcggat gttgaagaat acttgattaa
     3181 aatcgataac attcagaaac atcaggaaaa cattgaattc cctataataa aatcttccat
     3241 taatgacata gctaaaaaag atggtaaagg ccaaggaact tgttttctta taagctcaga
     3301 tggttattta gtgacaaatt accattgcat tgaaaacgct aaggaaatca caattaaggg
     3361 aattggttct gactacacta ccaaatatgg agttactatt gttgccactg acccttccaa
     3421 tgacttagct ttattaaaaa tttctaataa aaatttaaag ttctcaattc ccccatttgc
     3481 aattcgctca agcggtgtgg ctcaggcaga aaagatttat gcacttggtt ttcctcaaac
     3541 taaatcaatg ggggaagaat taaaaataac agaaggtatt attagttcaa aaagtggagc
     3601 tcaaggtgac ataagtaaat ttcaaatctc tgctgctgtg aatcctggaa atagcggagg
     3661 accacttatt gacgaagaag gtaacttagt tggggttatt tatgcaaaat cgaaagttgc
     3721 tgagtcggct ggatatgcca ttaaggcaag ttatttagag acatttctaa aaaatataga
     3781 cggatttgaa tttcctaatc tgactaatct aatcaaagaa aaaccgttga ctgaaaaagt
     3841 tgaaatattg aaagactata tattcatttt ggaatgcaat taattactgc ttttaacaag
     3901 cggatatcaa aaaatcaaat cggaggaaaa agattaaaac ggaaaaaaac gacgtcgtgt
     3961 tttcttcatg acaaatgtgg tttacgaagt cgaataagaa tgttaaaaac tcggtcaata
     4021 cttatctgaa aaacaacaac tcaaagcctg tatagccgtt tagatttgta aacagatgat
     4081 acatcctatg agaacataca tgaagctagc tttcctgggc ttaactttac agggaggaat
     4141 gctgttggca caaacacctg cagctcctaa aatgccggtt gatgagatat ccaaactcat
     4201 tacctacacc gaagtggtcg acgaacctgg tatgaacaaa gacacgcttt acaaccgtgg
     4261 actacgttgg ttcaaatcgt acttcaaaaa tcctaataaa gccatcaagc ttgctgatgc
     4321 cgaaggaaga acaattgatg gaggttatac atttactatc caacgtcccg acccagcttc
     4381 caaaaaaact cccgctccaa tggttgatgc aggattggtc aattttaaaa tgaagctgtt
     4441 tttcaaagac gcaaagttca aatacgaaat aaccaatatc tcttggcaac aaacatctta
     4501 ctatccaatc gaacgttgga tggataaaga agccaaaaac tatgatccta attatgcact
     4561 ttatctgcaa caaacagatc agtacatgaa aacactcata aaatcactcg aaacatttat
     4621 cgaaacagat cctctcaaga aaaaagacga ttggtgagat tttcaaacaa aatcgaaaac
     4681 cggaaagagc aatctattcc ggttttttta tgattatttt attgttgatt gactctttca
     4741 tccactccaa taaataaaaa agcctgaacc atttcgcatg attcaggctc atttatgttc
     4801 aaatgtttta ctgcgttatc acaattttct cgcttcttac tattctacca tcagcaatta
     4861 atcgcataag gtaaattcct gaattgacgt ttaatcctag ttttatcaaa ccggaatcgc
     4921 ttactaattg ttgttcttga ataaccctac cctgtaaatc acagaattca agttttgcct
     4981 gttttaccgt ctttgaaaca tgccatttca cagatactag acctgtggaa ggatttgggt
     5041 acgaaaccaa ctcagcatcc tccaaaagtg gatttgcaat gttaacagta aacgaatctg
     5101 ttacataaaa acgatctagt ccagcctcaa ctatatgacc attaggaaaa tctgttgctt
     5161 ccaatataaa cttcaccata cctggattag caatataatc gctcacttta aaaacttct
//
LOCUS       DAOOQW010000167         5218 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_19108_length_5218_cov_4.168313, whole genome shotgun sequence.
ACCESSION   DAOOQW010000167 DAOOQW010000000
VERSION     DAOOQW010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5218
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19108_length_5218_cov_4.168313"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2243
                     /locus_tag="PK281_08965"
     CDS             <1..2243
                     /locus_tag="PK281_08965"
                     /inference="COORDINATES: protein motif:HMM:NF014214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M36 family metallopeptidase"
                     /protein_id="HOY49518.1"
                     /translation="NGWHDLDGFTGAEETRSIGNNVSAYDDQDDDGFPDDYVEGGSSL
                     NFDFPYAATPNSNPLGNREAAITNLFYANNRVHDVLWYYGFNEEGGNFQNYNYTGGGL
                     DLDAVHAEAFDGSGTNNANFATPPDGESPRMQMYLWANNLGSYLTINSPSGIAGTFAS
                     AVAAFGMQMTTSPITAQVVEYNDGSINPSWACDFSLDWMDGKIALIDRGDCNFTTKVL
                     NAQDAGAIAVIMVNNQAGQPFSMGGEEDLITIPSIMVSQEDGNLIRAQLSSNVNATIN
                     INSLGNYFDSSFDNGVIAHEYGHGVSNRLTGGPSLADCLYNEEQMGEGWSDFLALIMT
                     TTSANSGEQARGIGNYVHGTSVNGPGIRPFPYSRDMTINPVVYDDIQVLSIPHGLGTV
                     WCSTLWDLYWNMVDVYGYDSDLLNGTGGNNKTIQLVMDGMALQPCSPGFIDGRDAILQ
                     ADELANNGTNKCLIWSTFARRGLGYTADQGSSESAFDGISAFDTPPFCSTVDFANFTT
                     SQTLVCAGSPVTFTDITQPVSESVEWTFEGGFPGSSINGTENVIFSSPGTYTVTLLST
                     TNLGSDDRTETITVVPQLSINAVVTDASLLVNNGAINITVTGGQAPYNTVWADIVSLN
                     DLTVNSLAPNVYEVTITDQAGCSLDTVFTVERPEGLEEINQVSFQINPNPFNNDLSLR
                     FENATQAELVRVYDLSGRILHQQSLSLQGKSTLTLPIAHLSAGTYIVQVQFSHQPIQS
                     KRVIKY"
     gene            2819..3670
                     /locus_tag="PK281_08970"
     CDS             2819..3670
                     /locus_tag="PK281_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006987962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOY49519.1"
                     /translation="MSEKSISPKSGALALVIALALIALGAWSIYAKLFIIAPFAFIAA
                     ILVLVGLTIVNPNESVVAVLFGSYAGTLKENGFFWMNPFLVKRKISLRARNNESQPIK
                     VNDKLGNPIMIGIVLVWRVENTFKAAFEVDNYELFVKIQSESAIRKLAGIYPYDSFDD
                     EHKEMSLRSGGDEINHQLERELSERLHMAGISVIEARISNLAYSQEIAGAMLQRQQAT
                     AIVAARTRIVEGAVGMVELALEHLSKRQIVELDDERKAAMVSNLLVVLCSDRAVSPVV
                     NTGTLNH"
     gene            3796..4293
                     /locus_tag="PK281_08975"
     CDS             3796..4293
                     /locus_tag="PK281_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49520.1"
                     /translation="MSHFQERQYLRSNPITWVAVVAMVIPIVIILATSAGEPAQDIFY
                     SVLFVTLFEGFFLTLLMIAHLDTDIDMNRIRFRWFPFQWKFNEINWSEVQSAEVRKYS
                     PLKEYGGWGLKGWTSKNRAYNVDGSIGIQLYLKNGHKVLIGTKQRESAEAFLYDLRIP
                     KPAHG"
     gene            4286..4483
                     /locus_tag="PK281_08980"
     CDS             4286..4483
                     /locus_tag="PK281_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011710220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HOY49521.1"
                     /translation="MAKKKPFVLRLDEDMLRDVEKWAADEFRSSNGQLEWIIREALIK
                     AGRLRKSSTTSDESEATGTEA"
     gene            4452..4973
                     /locus_tag="PK281_08985"
     CDS             4452..4973
                     /locus_tag="PK281_08985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49522.1"
                     /translation="MKAKLPELKRELESLPQPELVRLCLRIAKHKTENKELLDYLLWS
                     ANDPLLYAQEFKLEILLPFEMPFVNAYNFTKSIRKSLRLISKYLRFTGSRQGECELLL
                     ALVESYHTYYRREFRTAALSKIMFRCLKKAATNIAKLHEDIQADYTSNYNDLLRQTIT
                     RLGTEYALDQSYI"
     gene            4999..>5218
                     /locus_tag="PK281_08990"
     CDS             4999..>5218
                     /locus_tag="PK281_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49523.1"
                     /translation="MTGKRTYSLYINHKPLIDLQLNTWFTQAQITHAGKTYTLRRKGF
                     AGMNMQLRDDVGQLILEVKGGSFWKSSRN"
BASE COUNT         1492 a         1069 c         1104 g         1553 t
ORIGIN      
        1 ccaatggatg gcacgaccta gatggtttta ctggggctga ggaaacacgg tcgattggga
       61 acaatgtaag cgcctacgac gatcaggatg atgatggttt tccggatgat tatgtagaag
      121 gaggttcatc acttaatttc gattttcctt atgcagcaac accaaactca aatcctcttg
      181 gcaaccgcga agctgctatc accaatctgt tttacgctaa caaccgtgtt cacgatgtgc
      241 tttggtacta tgggtttaat gaagaaggtg ggaatttcca gaattataat tacactggtg
      301 gcggactaga tttagatgcc gttcatgctg aagcttttga tggtagtgga actaacaatg
      361 ccaattttgc tacgcctcct gatggtgaat caccccgaat gcaaatgtat ctctgggcga
      421 ataatttggg ttcatactta accattaact ctccttcagg cattgctgga acattcgctt
      481 cggcagtagc tgcatttggt atgcaaatga caacatcgcc cattacagct caagtggtgg
      541 agtacaacga tggaagtatt aatccttctt gggcatgtga tttttcgttg gattggatgg
      601 atgggaaaat tgcactcatc gatcgaggtg attgtaattt cacaacaaaa gtgcttaatg
      661 cgcaagatgc aggagctatt gcagttatca tggtgaataa tcaagctggt caacccttta
      721 gtatgggagg tgaggaagat ttaattacta ttccttccat catggtttct caggaggatg
      781 ggaatctgat ccgtgctcaa ctatcctcca atgtgaatgc tacaatcaat atcaactcat
      841 taggaaacta ctttgattcg agtttcgata atggtgtgat tgctcacgaa tatggacatg
      901 gtgtttcaaa tcgtcttact ggcggacctt cacttgccga ttgcttgtat aacgaagaac
      961 aaatgggtga gggctggagt gattttctag ctttaatcat gactaccaca tcagctaatt
     1021 cgggagaaca agctcgcggc atcggaaatt atgttcacgg aacttctgtt aatggtcccg
     1081 gaattcgtcc attcccatat tctcgggata tgacgattaa tcctgtggtt tacgatgata
     1141 ttcaagtgct ttcaattcca cacggtttag gaacggtttg gtgcagtacg ctttgggatt
     1201 tgtattggaa tatggtggat gtgtatggct acgattccga tttgcttaat ggaactggcg
     1261 gaaacaacaa aaccattcag ttagttatgg acggaatggc gttgcagccg tgttcgccgg
     1321 gatttattga tggtcgtgat gctattttac aagctgatga actagcaaac aatggaacaa
     1381 ataaatgtct tatttggagc accttcgcac gtcgtggttt aggatatact gctgatcaag
     1441 gaagctccga aagtgcattt gatggaattt ccgcattcga tactccacct ttttgctcaa
     1501 cagtcgattt tgccaatttt actaccagtc aaacccttgt atgtgcaggt tctccagtaa
     1561 ctttcaccga tatcacccaa cctgtttccg aatctgttga atggactttc gagggtggtt
     1621 ttccgggtag ttcgataaat ggaacagaaa acgtaatatt ttcaagccca ggaacataca
     1681 ctgttacgct tcttagcaca actaatctcg gtagcgacga tcgtacagaa acaattacgg
     1741 ttgttccaca attgtctatt aatgcagtgg tgaccgatgc ttctttgttg gttaacaacg
     1801 gtgctattaa tataacagta acaggaggac aagctccata caatacagtt tgggcggata
     1861 ttgttagctt aaatgatttg acggttaatt ccttagctcc taatgtatat gaagtaacca
     1921 ttaccgatca agcaggttgc tcactcgata ccgtttttac agttgagcgt cccgaaggtt
     1981 tggaggaaat caaccaagtt tcctttcaaa ttaatcccaa tccattcaac aacgatttaa
     2041 gccttcgttt cgaaaatgcc actcaagccg aactggttcg tgtgtatgat ttaagtggta
     2101 gaattcttca ccaacaaagc ctttcgctcc aagggaaatc taccttgact ttacccattg
     2161 cacatttatc ggcaggaacc tacattgttc aggttcagtt tagccaccaa cccattcaga
     2221 gcaaacgggt aattaaatac taattcagtg ttttaagtag cgaatcaaca tcactactaa
     2281 tgcaaacaga aacatgaaaa tgctaattcg gttcatgcca ttcatgaatc gaacattggc
     2341 attgggcttc ttctcgtttt tacgaaataa agtccggaca tctaggtaat aaagaatctt
     2401 ctttagaaac atcgcgttgg gctatcggtc aaagctacaa cagaattttt tattttatta
     2461 tttgggcatg ccacatttgg ccgcgctttt cattgcaaat cctctcgtat gccttcgcca
     2521 aggctatggc atacaatgcg ggttttccat tgcaatcgct catgcaatat atctcgttaa
     2581 ataaactagc tccaataact ctaactcgta ttcctcattt cgcatcccgg taaccccgga
     2641 ttatagaagg ctactgagtc atagaggtta cttaaagttc ttatctaatt tctagcatcc
     2701 cggatttaaa tccggggtta ccgagactat gaagcaatga ggtgctacga atatattttc
     2761 gaaaaatttg ttttctaata atttattatg atacctttgt gatatcaaat taatatcaat
     2821 gagcgaaaaa tctatttctc ctaaatctgg cgcactcgcc ttagtcattg cactcgcact
     2881 tatagcactt ggtgcttgga gcatttatgc aaaacttttt attatcgcgc cctttgcttt
     2941 tatcgcggca atcttggtct tggtcggact tacaatcgtt aaccccaacg aatcggtagt
     3001 agcggtactt tttggttcgt atgcaggtac attgaaagaa aacggcttct tttggatgaa
     3061 tccctttttg gtgaaacgta aaatttcact tcgtgctcgt aataacgaaa gtcaaccaat
     3121 caaggttaac gacaaacttg gtaatccaat catgattggg attgttcttg tttggcgagt
     3181 tgaaaacacc tttaaggcag ctttcgaagt cgataattat gagcttttcg taaagattca
     3241 aagtgaatca gctatccgaa aactagctgg aatctatccg tatgatagct tcgatgatga
     3301 gcacaaagaa atgtctttac gttcgggtgg agatgaaatc aatcatcaat tagaacgcga
     3361 actttctgaa cgacttcaca tggctggaat ttcagtaatt gaagcacgta ttagtaatct
     3421 ggcttattcg caagagattg ccggagccat gttgcaacgt cagcaagcta ctgcaatcgt
     3481 agccgctcgt acccgaatcg ttgaaggtgc agtaggaatg gttgaattgg ctttggaaca
     3541 cctttcaaaa cgtcaaattg tagaactcga cgacgaacgc aaagccgcca tggtaagtaa
     3601 tctactcgtt gttctttgct ccgatcgtgc tgtttctccc gttgtaaata ccggaacgtt
     3661 gaatcattga ttaagtacaa agtatcaagt acaaagtaca gagagacaag tacaaagttc
     3721 acagaatata gagtcaagta agtttttaaa atcttaatac ttaatactct atacttttta
     3781 ctataaataa ttcccatgtc ccactttcaa gaacgccaat atttacgatc caatccaatc
     3841 acttgggttg ctgttgttgc gatggtcatt ccgattgtga tcattttagc tacttctgct
     3901 ggagagccag ctcaagatat tttctactcg gttcttttcg tgaccttgtt tgaaggtttc
     3961 ttcctcacgt tgttgatgat agcacacctc gacacagata tagacatgaa tcgtatccgg
     4021 tttcgctggt ttccttttca atggaagttt aatgagataa actggtctga ggttcaatcc
     4081 gccgaagtgc gtaagtacag tcctttaaag gaatatggag gttggggatt gaaaggttgg
     4141 acttcgaaaa atagggcgta taatgtggat ggtagtattg gcattcagtt atatttgaaa
     4201 aacgggcata aagttcttat cggaaccaaa caacgtgaat cagcagaagc gtttttatac
     4261 gatctgcgta tcccgaaacc tgctcatggc taagaaaaaa ccatttgttc tacgtctcga
     4321 cgaagacatg ctccgtgatg tggagaaatg ggctgccgat gaatttcgca gttccaatgg
     4381 gcagttggaa tggattattc gagaagcatt aatcaaagca ggacgattac gaaaatcaag
     4441 tacaacaagc gatgaaagcg aagctaccgg aactgaagcg tgaactagag agtttgccac
     4501 agcccgaatt ggtgcgactt tgtttgcgta tcgccaagca taaaaccgaa aacaaagaac
     4561 tgctcgatta cctgctttgg tcggcaaacg atcccttgct gtatgcgcag gaattcaaat
     4621 tagaaattct gctaccgttc gaaatgcctt ttgtgaatgc ctataacttc accaaaagca
     4681 tccgaaaatc actccgactt atttctaaat acctccgttt tacaggttct cgtcagggcg
     4741 aatgcgaact tttactagcc ttggttgagt cttatcacac ctactaccga cgtgaatttc
     4801 ggactgccgc tttgtcaaaa atcatgtttc gttgtttgaa gaaagctgct actaacatcg
     4861 ccaaattgca cgaagacatt caagccgatt ataccagtaa ttacaacgat ttactccgtc
     4921 aaaccatcac ccgcttagga actgaatatg cccttgacca atcctatatc taattcgact
     4981 tcccctgaaa gatgggaaat gaccggtaaa cgtacgtaca gcttgtacat caaccataag
     5041 ccgctaattg acttgcagct aaacacctgg tttacacaag cgcaaattac ccatgctggg
     5101 aagacttaca cacttcgtcg caagggcttt gccggcatga atatgcaatt gcgagatgat
     5161 gttgggcaat taattctaga agtcaaaggt ggcagctttt ggaagtctag ccgtaata
//
LOCUS       DAOOQW010000168         5208 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_19164_length_5208_cov_4.181836, whole genome shotgun sequence.
ACCESSION   DAOOQW010000168 DAOOQW010000000
VERSION     DAOOQW010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5208
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19164_length_5208_cov_4.181836"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /locus_tag="PK281_08995"
     CDS             complement(<1..498)
                     /locus_tag="PK281_08995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HOY49524.1"
                     /translation="MENLEPLLSDSNDQHEILVRTYAGLEEVLEGEIRKLGGADVERM
                     VRSVICTGDKGFLYKLNLGLRTGLRVLKPIKKFRFRTNDDFYNEITAIDWPSLFDVDK
                     TMIFDTLLFSDHFTNSMFVSQLAKDAVCDRFRKDVGRRPFIDSKEPHIYVSVYVRDQE
                     VTIYLD"
     gene            626..2551
                     /locus_tag="PK281_09000"
     CDS             626..2551
                     /locus_tag="PK281_09000"
                     /inference="COORDINATES: protein motif:HMM:NF014522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HOY49525.1"
                     /translation="MKKILSFIAAVILAITTQAQTVVDIIVNSPDHTTLEAAVIAAGL
                     DGTLSGNGPFTVFAPTDAAFNALPAGTVQALLADIPALTDILTYHVVGATALSTSLSN
                     GQRIATFQGEKVTVTIAGTDVYINNAKVTVVDLVATNGVVHVIDAVLIPPTGTVVDVI
                     VNSPDHTTLETAVLAAGLEGTLSGDGPFTVFAPTDAAFAALPAGTIAALLADIPALTN
                     ILTYHAAGAAAFSSGLTNGQRVVTVQGEKVTVTIAGSNVYINNAKVTVADIIATNGVV
                     HVIDAVLLPPTGTIVDVVVNSPNHTTLEAAVLAANLQGTLSGDGPFTLFAPTDAAFAA
                     LPAGVLNSLLANVPALTSILTYHATSGAAFSAGLSNGQTIMTVQGQDVTVTFSSGNVF
                     INNAQVTVADIVATNGVVHVIDAVLLPDPGSVFDIISNSPVHTTLETAINAAGLATTL
                     DGPGSFTVFAPTDAAFAAVPQATLDALLADPNGLLTDVLLHHVVGSIEYSGSLSNGQQ
                     VPTLFGDNLTVTIAGPTVSIDNAVVAVTDLLANNGVVHVINAVLVPITLGINETTIPS
                     VVLYPNPVTESLRINLGETTKNATYQVVNQLGAVVSTGSFNQQISNLDVLDLNSGVYT
                     LRVQNEGNISTLRFVKN"
     gene            2651..3394
                     /locus_tag="PK281_09005"
     CDS             2651..3394
                     /locus_tag="PK281_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOY49526.1"
                     /translation="MECNQSAEWFKEWFNSPYYHILYTHRDVHEATQLIDHLIDYLKP
                     EVDSRMLDLACGRGRHSVYLHSKGFDVTGVDLSSESIRYAEDFEEEGLRFFVHDMRQL
                     LSTNAYDYVFNLFTSFGYFDRDEENVRVVRNMSDALKSGGCLVIDFLNVEKLRFSGIK
                     KEEKEIDGITFQIEREIREERVIKTIRFTDKGKEYYFEEKVMLLGAGEFERYFQACGL
                     EILNVFGNYQLDPFMPESSDRLIYCVRKP"
     gene            3391..4173
                     /locus_tag="PK281_09010"
     CDS             3391..4173
                     /locus_tag="PK281_09010"
                     /inference="COORDINATES: protein motif:HMM:NF014582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HOY49527.1"
                     /translation="MSVFWKIFILIGSVIASGSLVLAFRIKSGKTIKLLLSYSGAFIL
                     ALCLFHLLPEVYEGIADPLVSGWLILGGFLLQLLLDYISGGIEHGHIHPPDQHGHHNH
                     HHHHSHDHSHEDIDFHPVMRSPWLLMTGLCLHALVEGVPLMYGKAGDDLFTAIIFHNI
                     PISVSLMTLFLMSGKTIRQSLLALFIFSLMTPLGALTAVTLFPTESDFTGQFGFYALA
                     VVIGIFLHISTTILFESDEHHRFNIIKLITILLGFATALLIR"
     gene            4251..4883
                     /locus_tag="PK281_09015"
     CDS             4251..4883
                     /locus_tag="PK281_09015"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HOY49528.1"
                     /translation="MSVLVGKLAPAFSAQAVINGGEFVEDFSLEQYLGNKHVVFFFYP
                     KDFTFVCPTELHAFQDKLAEFEARNCAVVGCSTDTEQSHWGWLQVPKGKGGIEGIKYP
                     LVADTAKTISMNFGVLFGDYDYNEVGEMISTGPMIAFRGLFLIDKDGIVRHQVVNDLP
                     LGRNVDEALRMVDALQYFEENGEVCPANWRRGETAMKATHDGVASYLSGH"
     gene            5012..>5208
                     /locus_tag="PK281_09020"
     CDS             5012..>5208
                     /locus_tag="PK281_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016479876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HOY49529.1"
                     /translation="MADSNFVDYVKICCRSGKGGGGSMHLHRDKNTAKGGPDGGDGGR
                     GGHIILRGSTQVWTLIHLKYR"
BASE COUNT         1503 a          971 c         1106 g         1628 t
ORIGIN      
        1 atcgaggtaa attgtgactt cctgatcccg aacatataca cttacatata tatgaggttc
       61 tttggaatcg ataaaaggac gtcgcccaac atctttacga aatcgatcgc acacagcatc
      121 tttagccaat tgagaaacga acatactatt ggtgaaatgg tcgctaaaca agagcgtatc
      181 gaaaatcatg gttttatcaa catcaaacaa agaaggccag tcgatagccg tgatttcgtt
      241 gtagaaatcg tcgtttgtgc gaaatcggaa ctttttgatt ggctttagga cacgcaaacc
      301 ggttcgaaga cccaaattga gtttgtatag aaaaccttta tcaccagtac agattaccga
      361 gcggaccata cgttcgacat cggcaccacc caattttcgg atttctcctt cgagaacttc
      421 ctcaagacca gcgtatgttc taactagaat ttcgtgttgg tcattcgaat ctgaaagtaa
      481 aggctctaaa ttttccattt gataatcaat cacttaggtt gcaagatgct atttttgttt
      541 aatcttggag ttgatttgtt aaacaaattt taagcatgcc tgtttgatag cgcaatgtta
      601 acccaataaa cccacacgat taatcatgaa aaaaatttta tccttcattg ctgctgtcat
      661 acttgccatt acgacacagg cgcaaacggt tgttgatatc atagtcaaca gtccagatca
      721 cacaacttta gaagctgctg ttatagctgc aggattggat ggaaccttat cgggaaatgg
      781 accctttact gtatttgccc caaccgatgc cgcatttaat gccttgccag caggaacagt
      841 tcaagcatta ttggctgata tcccagcatt gaccgacatt ttaacttacc atgttgttgg
      901 agcaactgca ctatcaacat cactatcaaa cggtcaacga atcgcaactt ttcaaggaga
      961 aaaagttaca gtaacaattg cagggactga tgtttacatc aacaatgcaa aagtaacagt
     1021 tgtagattta gttgctacta atggagttgt acatgtgatt gatgctgtgt taattccacc
     1081 aactggaaca gtggttgacg tgattgttaa cagtcctgat catacaactt tagaaacagc
     1141 tgtattagcg gcaggtttag aagggacact ttctggtgat ggtccattta ccgtttttgc
     1201 accaaccgat gcggcatttg cagctttacc agcaggaact attgcagcgt tattagctga
     1261 cattccagca ttgacaaaca ttttaaccta tcatgcagca ggtgcagcag cattttcttc
     1321 aggtttaaca aacggacaac gtgttgttac ggttcaagga gagaaagtta cagttacaat
     1381 tgcaggatca aatgtgtaca tcaacaatgc gaaagtaaca gtagctgata ttattgcaac
     1441 taatggagtt gttcacgtaa ttgatgctgt attacttcct ccaacaggaa ctattgtaga
     1501 tgtggtagta aacagtccca accatactac attggaagca gctgtattag ctgccaattt
     1561 acaaggaaca ttatcgggtg atggcccatt tactttgttt gcaccaactg atgcagcatt
     1621 tgcagcatta ccagcaggag tattaaattc attattggcg aatgttccag cattgactag
     1681 catattaacg tatcatgcta cttcaggagc cgcattttca gctggattat ctaatggtca
     1741 aacgattatg actgttcaag ggcaagatgt aacagttact ttttcaagtg gaaatgtgtt
     1801 tattaataat gctcaagtaa ccgtagctga tattgttgca actaatggtg ttgttcatgt
     1861 gatagatgcg gtattacttc cagatccagg ttcagttttc gatattatct caaacagccc
     1921 agtacatacc acgttagaaa cagcaattaa tgctgccggt ttagctacta ctttggatgg
     1981 tcctggatca ttcaccgtat ttgctccaac cgatgctgca tttgctgcag ttcctcaagc
     2041 tacattggat gctttattgg cagatccaaa cggtctttta acagatgtgt tattgcacca
     2101 tgtggttgga agtattgaat attctggctc tttgtcaaat ggtcaacaag ttccaacttt
     2161 gtttggtgat aatttaacag taacgattgc tggtccaaca gtttcgattg acaatgcagt
     2221 tgtagctgtg actgatcttc ttgctaacaa tggagttgtt catgtgatta atgcggtgtt
     2281 ggttccaatt acattaggaa ttaatgagac aacaatccca tcagtggtat tgtatccaaa
     2341 tcctgtaact gaatctctac gtatcaactt aggtgaaaca actaaaaatg ctacatacca
     2401 agttgtgaat caattaggag ctgttgtttc tactggaagc ttcaatcaac aaatttccaa
     2461 cttagatgta ttggatttga attcaggagt ttacacgcta agagttcaaa acgaaggaaa
     2521 catttcgact ttgcgatttg taaaaaacta agtacaaaaa attaggaatt agaagaggct
     2581 gtcggtttat gacagccttt tctgttttat agctgtgtga aggccggctg aaattgtact
     2641 tttgccgacg atggaatgca atcagtcggc agaatggttt aaagaatggt ttaactcgcc
     2701 ttattatcac atcctatata cacatagaga tgtacatgag gcaacgcagt taattgacca
     2761 tttgattgac tatctcaaac cagaagttga ttcacgtatg ttggatttag cttgcggaag
     2821 aggtcggcat tcggtttatc tgcatagcaa aggattcgat gtgacaggcg ttgatttatc
     2881 gtctgaaagc attcgttatg ctgaagattt tgaggaagaa ggattacgat tctttgtcca
     2941 tgacatgcgt cagttattat cgacaaacgc ttatgattat gttttcaatc tatttaccag
     3001 ttttggttat ttcgatcggg atgaggaaaa tgttcgagta gttcgaaata tgtcggatgc
     3061 tttaaaaagt ggtggctgtt tggtgattga ttttttgaat gtggagaagc ttcggttctc
     3121 aggaatcaag aaagaggaga aggagattga tggtattact tttcagattg agcgtgagat
     3181 tcgtgaggag cgagtgatta aaaccatccg ttttactgat aaagggaaag aatattattt
     3241 tgaagaaaag gtcatgcttt taggtgccgg agaatttgaa cggtattttc aagcatgtgg
     3301 cttagaaata ttgaacgttt ttggtaatta tcagcttgat ccatttatgc cggaaagttc
     3361 ggatcgctta atctattgcg tgcgtaagcc atgagtgtgt tctggaagat atttattctg
     3421 ataggttctg ttatcgcaag cggaagtttg gttttagcct ttcgtattaa aagcggcaaa
     3481 accattaagt tgcttttgtc atacagtgga gcgttcattc tagcactctg cttatttcat
     3541 ctcttaccgg aagtttacga aggaattgcg gatcctttag ttagcggttg gttgatatta
     3601 ggaggattcc tattgcagct gttgctcgat tatatttcgg gaggaattga acatggtcac
     3661 attcatccac ccgaccaaca tggccatcac aaccatcacc accatcatag ccacgaccat
     3721 tcgcatgaag atattgattt tcatccagtt atgcgttcgc cttggttgtt gatgactggt
     3781 ttatgtttac atgctttagt agaaggtgtt cctttgatgt atggtaaagc aggtgatgat
     3841 ttattcactg ccatcatctt ccataatatt ccgatttctg tatctctcat gacactcttc
     3901 ttgatgtcgg gaaaaacgat tcgtcaaagt ttactagcac tatttatttt ctccttgatg
     3961 acaccacttg gtgcattaac agctgttacc ctcttcccta ccgaatcaga tttcacgggt
     4021 caatttggat tttatgcttt agcagttgta attggaattt tccttcacat cagcacaacg
     4081 attctttttg aaagcgacga acatcaccgt tttaacatca taaagctcat cacgattcta
     4141 ttaggatttg ctacagcctt gcttattcgc tgaacaaaag caaattcact gtgtttcctc
     4201 ggcggggaat gactaatttt gccccactaa aaaacttaaa accgatatac atgtcagtac
     4261 ttgttggaaa acttgctcct gccttttctg cacaggcagt cattaatggt ggagaattcg
     4321 ttgaagattt ctcactagaa caatacttag gaaacaagca cgtagttttc tttttctatc
     4381 caaaagattt cacatttgtt tgtcctaccg agctacacgc cttccaggat aaactggccg
     4441 aatttgaagc acgtaactgc gcagtggttg gttgttcaac cgatacagaa caatcgcatt
     4501 ggggttggct tcaagtacca aaaggaaaag gtggaattga aggaatcaaa tatcctttag
     4561 tagccgatac agctaaaact atttcgatga atttcggagt tttatttggc gattacgatt
     4621 acaacgaagt tggagaaatg atctcaacag gtccgatgat cgctttccgt ggattattcc
     4681 ttatcgacaa agacggaatt gttcgtcacc aagtggtgaa tgatcttcca ttaggtcgta
     4741 atgtggatga agcacttcgt atggtagatg ctttacaata ttttgaagaa aacggagaag
     4801 tttgtcctgc taactggcgt cgtggcgaaa cagctatgaa agctactcac gatggtgttg
     4861 ccagctattt atctggtcat taattaagaa cataagtata aaagtccctg ccgtttgggc
     4921 ggggattttt gtttttatcc cttccgattg aaggtgtaat tgtaatttcg cgaaaatccg
     4981 gttcatctat ccggaataaa aaaccaaaac tgtggcagat tctaattttg ttgattatgt
     5041 aaaaatttgc tgtcgctcag gcaaaggtgg tggaggttcc atgcacttgc atcgcgacaa
     5101 aaatacagct aagggcggtc cagatggtgg agatggtggt cgaggaggcc atatcatatt
     5161 acgaggcagt acgcaagttt ggacgttgat tcacttaaaa taccggaa
//
LOCUS       DAOOQW010000169         5175 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_19308_length_5175_cov_6.190430, whole genome shotgun sequence.
ACCESSION   DAOOQW010000169 DAOOQW010000000
VERSION     DAOOQW010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5175
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19308_length_5175_cov_6.190430"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1113
                     /locus_tag="PK281_09025"
     CDS             <1..1113
                     /locus_tag="PK281_09025"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOY49530.1"
                     /translation="LSGFLIDRFQVLSDKYYQDNKRLSIQRASWGTILAAISTAGYYA
                     AYVLILITTLAGSLSIGSLTFLSGSFARMRGLMETLLSRFASISQGALYLEDFYAFFE
                     LQPKIRKGEQSRPFPNPIREGFVFENVGFRYQNAASWALRNLNLHIRPGEKIALVGEN
                     GSGKTTLVKLIARLYDPVEGRILLDGHPLSEYNPDDLRREIGVIFQDFVKFQLTAGLN
                     IATGNIQDKNNQEKIDHAASMSLADSVVSTLPKGYDQVIGRRFAEGVDLSGGQWQKIA
                     LGRVYMRNAQVLILDEPTAALDAKAEHEVFERFASLSKGKTAFIISHRFSTVRMADRI
                     LVLDKGELIESGSHEELMKTEGHYAALFKLQAAGYR"
     gene            1172..2041
                     /locus_tag="PK281_09030"
     CDS             1172..2041
                     /locus_tag="PK281_09030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49531.1"
                     /translation="MRALSLYVLLFLSVSSTAQEITPVQRFDQTGKFWMYWGYNRAWY
                     TNSDIHIHGQDFDVNFYDVRAVDRPEKFGLKEYFSPTRLSIPQNNYRFGYVINQHWGV
                     SIGIDHMKYVVVNGQEVTMSGVVLPATNRSNNYVGSYINTPATLSRDLMRFEHTNGLN
                     LFSVDAEYTCRVAKLFKEKFGIYWQTNAGIGMLVPKTEAHLFSDTQFPEGYGIDNKYH
                     IAGYAASLRVGPEFRFLRNFFVKAQLRGGWIILPDVYINNEAPVRADHQFGFMQFNMT
                     AGGYFPIRLRKKH"
     gene            complement(2097..3044)
                     /locus_tag="PK281_09035"
     CDS             complement(2097..3044)
                     /locus_tag="PK281_09035"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOY49532.1"
                     /translation="MIYLRSFVLFLLLFPVAASMQARQHLKDSMQVVVPPDTIPPNLH
                     DLEEFLDEEDSSAVLEETESFEGIWDEDACVILNCDLYNAVWDTTRINPYGVNLKEKK
                     DSTYLTLFHDVNCDYAHPTCGEITSEFGFRRTRYHYGIDINLETGDEVLSVFEGTVRV
                     ARYSPTYGYYVIIRHLNGLETLYAHLSQIHVSAGHYVQAGDKVGLGGNTGRSRGSHLH
                     FEVRFLGQQINPRDIIAFEDYTCKNKEVCISPTSFDYLRKVEKQKAQAAARRYYKIRK
                     GDTLGRIARKNHTTVAKLCKMNKMSKRTKLKPGKRIRVA"
     gene            3168..3659
                     /locus_tag="PK281_09040"
     CDS             3168..3659
                     /locus_tag="PK281_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012781647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HOY49533.1"
                     /translation="MREAGVLLQKTNRRFLDQLKRNFPSDLDVRVRNFHDEVFENTDC
                     LTCGNCCRTTSPIFNQKDIEHLSRRLRIKTVVFVEKYLRIDEDKDYVLKSSPCPFLAE
                     DNTCDVYDDRPTACRGYPHTDRKRFHQLLDLTYENSFICPAVQAILELLKKDIGVNEK
                     RKF"
     gene            3695..>5175
                     /locus_tag="PK281_09045"
     CDS             3695..>5175
                     /locus_tag="PK281_09045"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOY49534.1"
                     /translation="MNRKILKALVQLFALVSRPGENAGRERSVVESFLKRQSGGEQLE
                     EYLREFDELVAELHRSTDETRARKRRSASSVKVLFICTQINEELTHSQKCLVYLRLIE
                     FLSASGEIDTPNLDFAQTVASIFTIPEDETEACLKFAGNSPQDSDSDACLWIHQSNEK
                     PKGDLRELKLDGLEAEMLVLYLKSAGSFYLRILQNSTTQLNGQALHTNRVYSLPQGAV
                     LRGNRMSPVYYSDILGCFLQQHASQKVIFQAKNIEYSFRNGKRGLHKFSFTETSGNLI
                     GIMGSSGAGKSTLLNLLNGNEKPSSGKILINGIDLNAAQTSTEGLIGYISQDDLLIEE
                     LSVFQNLWYNAKLCFADTSETELLRRVDHILDELGLNEIRDLKVGNPMNKTISGGQRK
                     RLNIALELIREPAVLFVDEPTSGLSSRDSENIMDLLKELALKGKLVFVVIHQPSSAIF
                     KMFDRLLLMDVGGYPVYYGNPVESVVYFKTIVDHANAGESECI"
BASE COUNT         1448 a         1076 c         1156 g         1495 t
ORIGIN      
        1 ctgtcgggat ttttgattga tcgatttcag gtgctttccg ataaatatta tcaagacaat
       61 aaacgattgt caattcagcg cgcttcatgg ggaacgatac tggcagcaat cagcacggct
      121 ggttactatg cagcttatgt attgattctt ataacaacgt tagctggcag tttgagtatt
      181 ggttcgctaa cctttctttc gggctcattt gctcgcatgc gcggattgat ggagactttg
      241 ctcagtcgct ttgcatcgat tagtcaggga gctttgtatt tggaggattt ttatgcgttt
      301 ttcgagttgc aacctaagat tcgaaaagga gaacaatctc gtccttttcc caatccgatt
      361 cgcgaaggtt ttgtgtttga aaatgtcgga tttcgctacc aaaatgcagc ttcttgggct
      421 ttacgtaatt tgaatttgca cattcgtccg ggagaaaaaa ttgctttggt gggtgaaaat
      481 ggttctggga aaaccacgct ggtcaagcta attgcccgtt tgtacgatcc tgttgaaggt
      541 cggattttgt tagatggaca tccattatcg gaatacaatc ccgacgattt acgccgagag
      601 attggggtga tttttcagga tttcgtgaaa ttccagttga cggcaggttt aaacatcgcc
      661 accggaaata ttcaggataa gaacaaccaa gagaaaattg atcacgcagc cagcatgagt
      721 ttagccgata gtgtggtgag tactttacct aaaggatacg atcaggttat tgggagacga
      781 tttgccgaag gcgttgattt gagtggcgga cagtggcaga aaattgcatt aggacgtgta
      841 tacatgcgta atgcacaagt gttgattctc gacgaaccaa cagcagcctt ggatgcgaaa
      901 gccgagcatg aagtatttga gcgttttgca agtttatcga aaggaaaaac agcatttatc
      961 atatcgcatc gtttctcgac cgttcgcatg gcagatcgga ttttagtact cgataaaggt
     1021 gaattaatcg aaagtggttc acacgaagaa ttgatgaaga ctgaaggaca ttatgccgcc
     1081 ttgtttaagc ttcaagcagc aggctaccgc tgataaaaat catagttcat ggggatttct
     1141 ttcccgtatc ttcgcagcct aaatcatttc catgcgcgca ttaagtcttt acgtgttgct
     1201 tttcttatct gttagttcaa cagcacagga aattactccc gttcaacgct tcgatcaaac
     1261 cggaaaattc tggatgtatt ggggttataa ccgcgcttgg tataccaaca gcgacattca
     1321 cattcacgga caagatttcg atgtgaactt ttacgatgta agagctgtag atcgcccgga
     1381 gaaatttggt ttgaaagaat attttagtcc tacacgctta tccattcctc agaacaacta
     1441 ccgttttggc tatgtaatca atcagcattg gggcgtctca attgggattg atcacatgaa
     1501 atacgtggtc gtaaatggac aagaagtaac gatgtcggga gttgtcttac cagctaccaa
     1561 tcgttcgaat aattatgtag gatcgtacat caacacgcct gctactttat cccgcgattt
     1621 aatgcgtttt gagcatacta acggcttgaa tttgtttagc gtagatgctg agtatacgtg
     1681 tcgtgtagcc aagttattca aagagaaatt cggaatttac tggcaaacga atgcaggtat
     1741 aggaatgtta gtacctaaaa cggaggcgca tttgttttcg gatacacaat tccctgaagg
     1801 ctatggcatc gacaacaaat accacattgc tggttatgca gcgtcgttgc gtgttggacc
     1861 tgagtttcgt ttccttcgta atttctttgt aaaagctcag ttacgaggcg gctggataat
     1921 tttacctgat gtatacatca ataacgaagc tcctgttcgt gctgatcatc aatttggttt
     1981 catgcaattc aacatgacgg ctggaggata ttttcctatt cgtttgagga agaagcattg
     2041 atatgctaga aaactttaat ttcttctgct aagaaataat tgcatctgat taaaaactaa
     2101 gcaacccgaa ttctttttcc cggcttcagt ttggtgcgtt tactcatttt attcatctta
     2161 cacagtttcg ctactgttgt atgattcttc cgtgcaattc ttcccaaagt atctcctttg
     2221 cgaattttgt aataacgacg ggctgctgct tgtgctttct gcttttcaac tttgcgtaaa
     2281 taatcgaaac tagtcggact aatacatact tccttgttct tgcaagtata atcctcaaag
     2341 gcaatgatat cgcgtggatt gatctgttga cctaagaatc gcacttcaaa atgcaagtga
     2401 ctaccacgtg aacggcctgt atttccacct aatccaactt tatcgcccgc ttgaacataa
     2461 tgtccggctg aaacgtgaat ctgggaaagg tgagcatata atgtttcaag cccatttaaa
     2521 tggcggatga tgacataata gccatacgtt ggactataac gggcaacacg aaccgttcct
     2581 tcgaataccg aaagtacttc gtcacccgtt tcgagattga tgtcaattcc gtagtgataa
     2641 cgcgttcggc ggaagccaaa ttcggaagtg atttccccac aagttggatg cgcgtaatca
     2701 cagttaacat catgaaaaag cgttaaatag gtagaatctt tcttctcctt taaattaaca
     2761 ccatacggat tgattcgagt cgtatcccaa actgcattat ataagtcaca attcagaatt
     2821 acacaggcgt cttcatccca aatcccttca aaagattccg tttcttctaa tacagcagac
     2881 gaatcttcct catcgagaaa ttcttctaaa tcgtgtaaat tgggtggaat cgtatcgggc
     2941 ggaactacta cttgcatcga gtcttttaag tgttgccggg cttgcatgga agcagccaca
     3001 ggaaagagca acagaaaaag aacaaacgat cgtaaatata tcatgcaggt gtagaacggt
     3061 aaagactcta tttttgtatc ggggtggcaa aaataaactc tccattcccg actgacaagt
     3121 taaaaactcc caaattgtca acaaacccaa aatatcaacc cgaagccatt cgtgaagctg
     3181 gtgtattgct tcagaaaacc aaccgacgct tcctcgatca actcaaacgt aactttccat
     3241 ccgacctcga tgtgcgggtg cgtaactttc atgatgaggt ttttgaaaat accgattgcc
     3301 ttacctgtgg caactgttgc agaacgacca gccccatctt caaccaaaaa gatatcgaac
     3361 atctttcccg tcgattgcgc atcaaaacag ttgtatttgt agagaaatac ctgagaattg
     3421 atgaggataa agattacgtg ttgaagtcat cgccctgccc atttctagcc gaagacaata
     3481 cctgtgatgt gtacgatgat cgcccaacag cctgtcgtgg atatccgcat accgatcgaa
     3541 aacgctttca ccagttgctc gatttgacgt atgaaaacag cttcatttgt ccggcagtgc
     3601 aggctatttt ggaattactc aagaaagata taggtgtgaa cgagaagcgg aaattctgag
     3661 tgtgcgcctg attttgtagt tttatcggca gagcatgaat cggaaaatcc tcaaagcttt
     3721 agtacaatta ttcgcactgg tttcacgacc gggtgagaat gctgggcgcg aacgttcggt
     3781 ggtagagagc tttctcaaac gtcaatcggg aggtgagcag ctagaagaat atctaagaga
     3841 attcgatgag ctcgttgccg aattgcatcg cagcaccgac gaaacacgcg cgcgcaaacg
     3901 tcgttcggca tcttctgtta aagtgctttt tatctgtacg cagattaatg aggagctaac
     3961 acattcgcaa aaatgcttgg tatatctccg cttgattgag tttctatcag caagtggtga
     4021 gattgataca cccaatttag atttcgctca aacggtagca tctattttca cgattccgga
     4081 agatgaaacc gaagcctgtt tgaagtttgc ggggaattcg ccccaagatt ccgattcgga
     4141 tgcttgcttg tggatacatc aatcgaatga aaagcccaaa ggggatttac gtgaactcaa
     4201 attggatggt ttagaggccg agatgttagt tctctacctc aaaagtgctg gtagttttta
     4261 tctgcgaatc ctgcaaaata gcaccacaca gctcaacgga caagcattac acaccaatcg
     4321 tgtttattcc ttgcctcaag gtgccgtgtt gcgaggcaat cgtatgtcgc ccgtgtatta
     4381 cagcgatatt ctcggttgtt ttcttcagca acatgcttcg cagaaagtca ttttccaagc
     4441 aaaaaacatc gagtattcct ttcggaacgg aaagcgtggt ttgcacaaat tcagtttcac
     4501 cgaaacttct ggaaacctca ttggtatcat gggatcgagc ggtgccggga aatcgacctt
     4561 gctcaatttg ctcaacggaa atgagaagcc tagttcaggg aaaattctca tcaacggaat
     4621 cgacctcaac gcggctcaaa ccagtacaga aggcttaatt ggttatatct cgcaagatga
     4681 tttgctgatt gaggaattga gcgtgttcca aaatctttgg tacaatgcca aattatgctt
     4741 tgctgatact tccgaaaccg aattacttcg tcgggtagat catatactcg atgagctcgg
     4801 attaaacgaa atccgtgatc tgaaagtggg aaatcccatg aataagacca tcagcggcgg
     4861 acaacggaaa cgtctcaaca ttgcactcga gctcattcgt gaaccggctg ttctatttgt
     4921 cgatgaacct acttccggac ttagctctcg cgattccgaa aacatcatgg atttgctcaa
     4981 agagctggca ttgaagggaa agctcgtctt tgtagtcatt caccaaccat cctcggccat
     5041 tttcaaaatg ttcgatcgtt tgttattgat ggatgtgggc ggttatccgg tgtattatgg
     5101 gaatccggtt gaatcggttg tctattttaa aacgattgtc gatcacgcga atgcgggtga
     5161 aagtgagtgt atcga
//
LOCUS       DAOOQW010000170         5124 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_19565_length_5124_cov_5.383310, whole genome shotgun sequence.
ACCESSION   DAOOQW010000170 DAOOQW010000000
VERSION     DAOOQW010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5124
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19565_length_5124_cov_5.383310"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="PK281_09050"
     CDS             complement(<1..303)
                     /locus_tag="PK281_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49535.1"
                     /translation="MKIKILFILLLSGFITFGQSKKEKEQAQKDAQAKLDTLTKSNAA
                     LTIKSDSLSKELEKYFGLYTVIKDKVVKKDFDPAKMAAIIDSLKTGRDSLTLKLAAA"
     gene            complement(390..1703)
                     /locus_tag="PK281_09055"
     CDS             complement(390..1703)
                     /locus_tag="PK281_09055"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HOY49536.1"
                     /translation="MYLSGSGINRVTQLVCLLVVILLIPFASIAQQKPDVIPDGTQGE
                     MLEVLQTDTVPDKWRDKRWRLFPGRFTSFKFGGGFLYEFAGYKQDGVAKQQMDSIGSV
                     LEHAFSVRDFRITASGKLKTKRTITWKAGFMYDDANRQWFVRETGMMIDVPELWGKFF
                     IGRTKEGFSMNKVMVGYAGWTMERQMAIDVIPILADGIKWLGYLPKPRIFWNAGAYAD
                     WLSKGQSFSTFKWQVAVRTGFLPLYRPEKNEVFHIGVDYRYGVPVKDEIRLRSRPEAD
                     PSFHFIDTGKFPSDYSNHIGGEAYYCRGPLMLGSEIYWHGFHSTQVNDPMFFGGEVVA
                     SYMLTGESRPYYTSTAILGFVPVKRSVFKGGPGAWEVLVRASTLDLVGGTIQGGKFWR
                     ITPMVNWYLSKDIRLDLVYGYGVLNRFGLKGANQFFQSRIQLTLL"
     gene            complement(1726..2325)
                     /locus_tag="PK281_09060"
     CDS             complement(1726..2325)
                     /locus_tag="PK281_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49537.1"
                     /translation="MKKIFLTFALGLLLIGGLKAQTNSVAAITYSIGFGSGDMGDFIS
                     QPSFRGANFDFRKIIAPNIGVGFSIGWNTFYQSQSKDSYTSGNTTLTGKQYRYSNNVP
                     MLASATYFIKPGEKINPFVGLGIGTIYTRRNTDMNLYTLEQEAWNFALQPEVGVLYSL
                     NDQAAITLSGKFNQGFKAGSELTESQSFFTLNIGFAFIK"
     gene            complement(2336..2953)
                     /locus_tag="PK281_09065"
     CDS             complement(2336..2953)
                     /locus_tag="PK281_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HOY49538.1"
                     /translation="MKRFLLMIVVTSMMWGCYPGGAEFVEDTDAVLTTFDSKFDFASK
                     NKYARPDKIVVDVEIDNGDTTYVYMKDVFAQTIFDAIDENMEELGWTLVDISEEPDLL
                     LNPAAIKSTTYFYSYWYDWWYGGWYGGYWGWYYPPYYTISSYTTGSLIFTLVDPNAGE
                     DSPINQSQASWVAVMNGLYVGTYNVSRVTTAIDDAFEQSPYLKTN"
     gene            complement(3022..4239)
                     /locus_tag="PK281_09070"
     CDS             complement(3022..4239)
                     /locus_tag="PK281_09070"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HOY49539.1"
                     /translation="MKSFKSIKWILLFLILPAFSWAQKTERQVGDTTKYKTLIPEEKQ
                     GLLKNVSVIANMNFALRNEFVDNEYTKTRFRNEQFRLEIRGQVHEKVYFRFRDRYTRA
                     QTSESIDNLSRSVDLAYLRFDLSEKFSLSAGKMCADWGAWEFDWNPIDIYEYSDIVEY
                     ADNFLTGVGATFTPNAKHQFTFQVLDSRTKSFDELYGESQPNFTESKVPLAFVANWRG
                     NLFDGKFKTIWSYSLFNEAQDLDGKSANMNYIALGNELNLGKFRFIYDLKWSLEDLDR
                     TTVVSETIKASGLYDYSVANTTYVGHWVNFRYRITPKVDFTLVGMLDIARWKNSDNDP
                     SNTTGETHIRNAWGYIPAIEYYPWSDLNIKFFANWVGRVYKYSDYAKTSLGASDYTTG
                     RFTVGFISPLGIL"
     gene            complement(4292..>5124)
                     /locus_tag="PK281_09075"
     CDS             complement(4292..>5124)
                     /locus_tag="PK281_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HOY49540.1"
                     /translation="EGQDAVPMTVGQEFHAFGNQLDAEIAALKRAETFLYEQNMGATA
                     IGTGITASPGYAEKCAVHLAKITGKPIVMSKDLIAATSSQQGFVVYSSALKSYAIALS
                     KISSDLIFLASGPRTGIFEINLPALQPGSSIMPGKVNPVMPELMNEVCFKVIGNDLTV
                     TLAAHSGLLQLNAFEPVEAIAMFESQGLLFKSLPLFRKQCIDGITVNEDVQKRNIERS
                     IGIVTALNPVLGYEKTTELAKEALQSGKGILELIREKKLLTEDQIKKLMDPVTLTGQK
                     "
BASE COUNT         1446 a         1124 c         1010 g         1544 t
ORIGIN      
        1 tgctgctgca agctttagcg taagcgagtc acgaccggtt tttaatgagt caataatggc
       61 agccatttta gccggatcaa aatccttctt tactacctta tctttaatga cggtgtataa
      121 tccaaaatac ttctctaatt ctttcgatag tgaatctgac tttattgtca atgctgcatt
      181 cgacttggtt aaggtatcaa gcttggcttg cgcatctttt tgagcttgtt ctttttcttt
      241 cttgctttgt ccgaaggtta tgaaaccact caagaggaga ataaaaagaa ttttaatttt
      301 catgatatag gtgtttaggg ataggtagaa ttactgcttt aatataagca atatcgtttg
      361 aatgatttgc actttgacat gtcgcgctac tataataatg tgagttgaat tcgactttga
      421 aaaaattgat tggcaccttt taatccgaag cgattcaaca ctccatatcc ataaactaaa
      481 tcaaggcgta tgtctttgga taaataccag ttcaccatcg gtgtgatcct ccagaatttt
      541 cctccttgta tcgttccacc aacaaggtcg agggttgaag cgcgcactaa aacttcccat
      601 gcaccaggtc ctcctttaaa cacagacctt ttcacaggca caaaaccaag tatagcggtc
      661 gaagtataat atggacggga ttcgcctgta agcatgtagc tggctacaac ttctccacca
      721 aagaacattg gatcgtttac ttgtgttgaa tgaaagccat gccagtagat ttctgatccg
      781 agcatcaaag gaccacgaca gtagtacgct tcaccaccaa tgtgatttga ataatcggat
      841 ggaaatttac ctgtatcaat gaagtgaaaa gaaggatcag cttctggtcg tgaccgaaga
      901 cgaatttcat ctttcacagg aacgccatat cggtagtcaa cgccaatgtg aaacacttca
      961 tttttttccg gtcggtacag gggcaagaat ccagtgcgga cagcaacctg ccatttgaac
     1021 gtagagaatg attgaccttt tgataaccag tcagcatagg cgcccgcatt ccagaatatt
     1081 cgcggcttcg gtaaatagcc aagccatttt attccatcgg caagaatcgg aatgacatca
     1141 atcgccattt gccgttccat tgtccagccg gcataaccaa ccatgacttt gttcattgag
     1201 aatccttctt tggtgcggcc aatgaaaaac tttccccaga gctcaggtac atcgatcatc
     1261 attcctgttt cgcgcacaaa ccattggcga ttggcatcat cgtacataaa gccagccttc
     1321 cacgtaatgg ttcgtttagt ttttaatttc ccgctcgcag taattcggaa gtcacgaacc
     1381 gagaaagcat gttcaagcac actgccgatg gaatccattt gttgctttgc aactccatct
     1441 tgcttgtacc ctgcgaattc gtagagaaat cccccaccaa atttgaaaga agtaaaccgc
     1501 ccaggaaata atctccaacg cttgtcgcgc catttatcgg ggacagtgtc ggtctgtaac
     1561 acttccaaca tttcaccctg tgttccgtct ggtatcacat cgggtttttg ttgggcaatc
     1621 gaagcaaacg gaattaacag aattacaacc aataggcaaa ccagttgggt aaccctgtta
     1681 atgccgctgc cagataggta catgatgcaa tgcttacaac tttaattatt taataaatgc
     1741 aaatccaata ttcaaggtga agaacgattg tgattcagtt agctcgctac cggccttaaa
     1801 tccttgatta aattttccag acagagtaat ggcagcttga tcattcaacg aatacaatac
     1861 tcctacttca ggttgtaacg cgaaattcca tgcttcctgt tccagtgtgt acaaattcat
     1921 atcggtgttt cttcgggtat aaattgttcc aatacccagt ccgacaaatg gattaatttt
     1981 ttcaccaggc tttataaaat acgttgcgct cgccagcatt ggcacgttgt tgctgtagcg
     2041 atattgcttt cctgtaaggg tggtattacc agaggtgtag gaatctttac tttgggactg
     2101 gtagaaagta ttccagccta ttgaaaagcc cacaccaata tttggtgcaa ttatttttct
     2161 aaaatcgaaa ttagcaccac ggaaactcgg ttgcgaaata aaatctccca tatcgcctga
     2221 accaaaaccg attgagtatg taatggcagc aacactgttg gtctgagctt tgagccctcc
     2281 aattaaaaga aggccaagag cgaatgttaa aaatattttt ttcatggcgt tttaattaat
     2341 tggttttaag ataaggtgac tgttcaaagg catcatcgat cgcagttgtg acgcggctga
     2401 cattgtatgt gcctacataa agtccattca taacagcaac ccatgaagct tgcgattggt
     2461 tgatcggact gtcttctccg gcattcggat caaccagtgt aaagatcaat gaacctgtag
     2521 tatagctgct gatggtgtag tatggaggat aataccatcc ccaataacca ccataccatc
     2581 caccatacca ccagtcatac cagtaactgt aaaagtatgt tgtgctcttg atggctgccg
     2641 gattgagaag aagatccggt tcctcgctga tgtccaccaa tgtccaaccc aactcttcca
     2701 tgttttcatc aatcgcatca aagatggttt gggcaaaaac atctttcatg tagacatagg
     2761 ttgtgtctcc attgtcgatc tcaacatcaa caacaatctt atctggtctg gcatacttat
     2821 tcttgctcgc aaaatcaaat ttgctatcga atgttgtcaa tacggcatcg gtgtcttcga
     2881 caaactcagc tccaccggga tagcaacccc acatcatgga agtcaccaca atcattaaga
     2941 gaaatcgttt catagttttc atcagtttgt ttttattttt tttgcaaacg tacgaggttg
     3001 tatttaattg ttaaagtgtt tttacaggat acctaatggt gaaataaatc caacagtaaa
     3061 tctaccagta gtgtaatcag atgcacctaa actagtttta gcgtagtcag aatatttata
     3121 tacacgacct acccagttag cgaaaaattt gatattcaaa tcgctccatg gataatattc
     3181 aatggctgga atataacccc aggcgttacg aatatgtgtt tcacctgtcg tatttgatgg
     3241 atcattatca ctgttcttcc aacgcgcaat gtccaacata cccaccagcg taaaatcaac
     3301 tttgggggtt atgcgataac gaaagtttac ccagtggcca acgtacgtgg tgttggccac
     3361 cgaatagtca tataagccac tcgctttaat agtttcactc acgacagtcg ttctatctaa
     3421 atcttcaagg ctccatttta aatcgtagat gaatctgaat ttaccgaggt taagctcatt
     3481 acccaaagcg atgtaattca tgttcgcact tttaccatct aaatcctgag cttcattaaa
     3541 taaagagtaa gaccagatgg ttttaaattt tccatcgaac aagttacctc gccagttcgc
     3601 tacaaaagcc aatggtactt ttgattctgt aaagtttggc tgactctcgc catacaattc
     3661 atcaaaggat ttggttctgg aatccagcac ctgaaaagta aattggtgct tggcgttagg
     3721 agtgaaggtg gctccaacac cggtaagaaa gttatctgcg tactcaacaa tatcagaata
     3781 ctcatagatg tcaattgggt tccaatcaaa ttcccatgcg ccccagtcag cacacatttt
     3841 tccagcagag agactgaatt tttcagataa atcaaatcgc agatacgcaa ggtctacaga
     3901 acgactgagg ttgtcaatgg attctgatgt ctgagcacga gtgtaacgat cgcgaaaacg
     3961 gaagtacact ttttcatgta cctggccccg gatctctaat ctgaattgtt catttctgaa
     4021 acgtgtcttt gtgtattcgt tgtcaacaaa ttcattgcga agcgcgaagt tcatgttcgc
     4081 aatcacactc acgttcttta atagcccctg cttttcttca ggaatcagtg ttttgtattt
     4141 ggtagtatct cctacttgtc gttccgtttt ttgtgcccaa ctgaatgcag gtaggatcaa
     4201 aaacaataga atccatttta tgcttttgaa tgatttcata acattatcgc ttaaagtcta
     4261 ttcatcaaag tcagtttcgt ttttgatatt actatttctg ccctgttaat gtaacaggat
     4321 ccattagctt tttaatctga tcttcggtca atagcttttt ctcacggata agttcaagaa
     4381 tacctttgcc gctttgaagt gcttccttcg cgagttccgt tgttttttca tatccgagaa
     4441 caggattgag tgcggtaaca atacctatgc tgcgttcaat gtttcttttt tgaacatctt
     4501 cgttcaccgt aattccgtca atacactgct tgcggaaaag tggtaaggat ttgaacaaca
     4561 agccttgtga ctcaaacatg gcaatcgctt caaccggttc aaatgcatta agctgaagaa
     4621 gtcctgagtg tgctgcaagt gttaccgtca gatcatttcc aattaccttg aaacagactt
     4681 cattcatcaa ctcaggcatc accggattta cttttccggg catgatggaa gaacctggtt
     4741 gtagggcagg aagattgatt tcgaaaatac ctgtacgtgg accagatgcc aggaaaatta
     4801 agtcactgct gattttagat agtgcaatcg catagctttt taatgctgat gagtatacta
     4861 caaatccttg ctgactgcta gttgctgcga taaggtcttt gctcatcacg atcggcttac
     4921 cagtgatttt cgctaggtgc acggcacatt tttcagcgta tcctggtgaa gcggtgatgc
     4981 cggtacctat ggccgttgcg cccatgtttt gctcatacag aaaagtttct gcacgcttca
     5041 atgctgcgat ttcagcgtca agctgattgc caaaggcatg aaactcctga ccaaccgtca
     5101 tcggtaccgc atcttgacct tcgg
//
LOCUS       DAOOQW010000171         5098 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_19685_length_5098_cov_5.018441, whole genome shotgun sequence.
ACCESSION   DAOOQW010000171 DAOOQW010000000
VERSION     DAOOQW010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5098
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19685_length_5098_cov_5.018441"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1055
                     /locus_tag="PK281_09080"
     CDS             <1..1055
                     /locus_tag="PK281_09080"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOY49541.1"
                     /translation="DGDGIPNGRDECPDKPGTISLNGCPDSDHDYIADKYDECPTDPG
                     SVELKGCPDKDNDGIPDKLDRCPEEAGKPEFNGCPDQDEDGVTDKEDQCPNLPGFAYL
                     GGCPDRDLDSIPDKEDLCPDEAGPKSLSGCPDSDADGLIDSKDQCPGEAGPMITKGCP
                     DKDSDGFADYQDLCPDVAGKNQGCPDKKVLITEPNPNLRDQDGDGLSDAIDKCPTEAG
                     SLQNNGCPQLNKQEQAILNSAFANLEFETGKSLIKESSYESLDELAELMVSKPEWRIL
                     FSGHTDNVGSPASNLNLSKNRALALKTYMISKNIPAERVLTEWFGQTKPIASNKTPEG
                     RQKNRRVEITVLTLTK"
     gene            1064..2056
                     /locus_tag="PK281_09085"
     CDS             1064..2056
                     /locus_tag="PK281_09085"
                     /inference="COORDINATES: protein motif:HMM:NF036967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HOY49542.1"
                     /translation="MGEHFRRRKKIQEQEFGFGNTGQKKRLLNPDGSFNVVRKGLSFF
                     ESFNPYNELLTSSWKRFFLFVLLWYFTINMTFAGIYLIFGIEHLKGIDPAEESGPFWE
                     AFFFSAQTLSTVGYGRISPSGILVNSIAAFEALIGVTSFALITGLVFGRFSKPVGKLM
                     FTENILMSPYKNNQTALMFRLTNRMKSQLVNLSANVSSSLNLVDENGVLARRFFRLKL
                     ERDEIVFFPTSWTIVHPIDENSPFYGMTEEEFRASEPELLILIQGYDDSYFANIQVRY
                     SYIEENTVWGGKWRKILQVAEDGSSEIHLNHFNDFDRVEINSLIPDKSIREIAD"
     gene            complement(2128..2382)
                     /gene="rpsT"
                     /locus_tag="PK281_09090"
     CDS             complement(2128..2382)
                     /gene="rpsT"
                     /locus_tag="PK281_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOY49543.1"
                     /translation="MANHPSAIKRIRSNDAKRLRNRYFHKTTRNALKKLRLTSDKKEA
                     QAQLADVSSMLDKLAKRSIIHKNKAGNLVGKITRYVNSLK"
     gene            complement(2407..2478)
                     /locus_tag="PK281_09095"
     tRNA            complement(2407..2478)
                     /locus_tag="PK281_09095"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2443..2445),aa:Glu,seq:ttc)
     gene            2675..3985
                     /gene="mtaB"
                     /locus_tag="PK281_09100"
     CDS             2675..3985
                     /gene="mtaB"
                     /locus_tag="PK281_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HOY49544.1"
                     /translation="MNTKKRVAFYTLGCKLNFSETSTVARLFQGAGFDKVEFDDSPDI
                     YVINTCSVTDNADKKCKSLVNRALRVSPHAYVAIIGCYAQLKPEEIAAIPGVDLVLGA
                     SEKFNILEHMEEMEKNVKTQIHACEIDAVNTFVPSYSSGDRTRTFFKIQDGCDYSCSF
                     CTIPLARGKSRSESIAFTLDAARKLIQETGSREIILTGVNTGDFGRHNGERFIDLIKE
                     LDLLEGIDRFRISSIEPNLLSDEVIEFVANSNKFVPHFHIPLQSGSNATLRRMRRRYQ
                     RDVYESRVALIKQLMPDCCIGVDVIVGFPGETEEEFLESYTFIQELDVSYLHVFTYSE
                     RPNTLAIEMPDVVSDKDRAKRSKMLHILSDKKKRYFYQHQLTKTKQVLWEGERAGDWM
                     YGFTENYVKVKTPYQPEKTNTLEWICLQDIDSEGICTATSLIPA"
     gene            4014..>5098
                     /locus_tag="PK281_09105"
     CDS             4014..>5098
                     /locus_tag="PK281_09105"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOY49545.1"
                     /translation="MYATISELLKSFGIDIPLPIQTFGFFVAIAFIVAAYLLSSELRR
                     KEALGLFKPSFRKVETGKEAGTFDYILAGLIGFLIGFKAFYMIMNYSEFVDDPQSTLL
                     SGKGNFIGGLLVAAAMIYLRYRESESQKKQTRATVQMPLKAEDHVGNIILLGVIGGLL
                     GAKIFHNLENPSEFMADPIGALLSFSGLTFYGGLIVASILIIRYGIKNGLPALHLTDA
                     AAPALMLSYGVGRIGCMMSGDGDWGIVNNNPKPGWMSALPDWMWSFKFPHNVNNEGIP
                     IPGCVGRWCHELPEAVYPTSFYETLMAIVLFGILWMIRKRIQTPGMLFSIYLILNGAE
                     RFLIESIRVNTQYHIMGMGITQAQIIST"
BASE COUNT         1567 a          932 c         1105 g         1494 t
ORIGIN      
        1 cagacggtga tggaattccc aatggaagag atgaatgtcc cgataaacct ggaacaatta
       61 gtttaaatgg ttgtcccgat tctgatcacg attacattgc cgataaatat gatgaatgcc
      121 ctacagatcc gggtagcgta gagttaaaag gttgtcctga taaggataat gacggcattc
      181 cggataaact cgatcgttgt ccagaagaag ccggaaaacc tgaatttaac ggttgtcctg
      241 atcaggatga agatggtgtg acggacaaag aagaccaatg tcctaatcta cctggctttg
      301 cctatttagg tggatgcccc gatcgagatt tagatagcat acctgataaa gaagatttgt
      361 gtccggatga agcaggacca aaatcacttt cgggttgccc tgattcggat gcagatggtt
      421 taattgattc gaaagatcaa tgcccaggag aagctggtcc aatgataacg aaaggctgcc
      481 cggataaaga ttcggatggt tttgcggatt atcaagatct atgtccggat gttgctggta
      541 agaaccaagg ttgtccggat aagaaagtat taattacaga acctaatccc aatttacgag
      601 atcaagatgg agatgggttg agtgatgcaa ttgataaatg tccaacagaa gccggaagtt
      661 tgcaaaacaa tggttgtccg cagttgaaca agcaagaaca ggccattttg aattcggcat
      721 ttgctaattt ggaatttgaa acgggcaaat cgctcataaa agaatcatcg tatgaatcat
      781 tagatgaatt agctgagctg atggtatcaa aaccagaatg gagaatcctc ttttcgggac
      841 ataccgacaa tgtaggatca cctgctagca acctcaacct ttcaaagaat cgggcattgg
      901 cgcttaaaac ctatatgata agtaagaaca ttccagcaga gcgagtgtta accgaatggt
      961 ttggacaaac gaagccgatt gctagtaaca aaactccaga agggcgacaa aaaaacagac
     1021 gagttgaaat aacggtgctt accctaacca agtaaaatta ttcatgggcg aacattttag
     1081 aagaagaaaa aagatacaag aacaagagtt tggatttggt aataccggac aaaagaagcg
     1141 actactaaat cccgatggat cttttaatgt ggtgaggaaa ggactctcgt ttttcgaatc
     1201 gttcaatcct tataatgagc tattaacatc ctcttggaaa cgatttttcc tgtttgtgtt
     1261 gctttggtat tttactatca acatgacatt tgcaggcatt tatctaatat tcggaatcga
     1321 gcatttaaaa ggaattgatc cagccgaaga aagcggacca ttttgggaag ccttcttttt
     1381 tagtgcgcaa acacttagta ctgtaggata tggaaggatt agtccaagtg gaatattagt
     1441 gaattctatt gcagcttttg aagcattgat tggtgttacc agttttgctt taattaccgg
     1501 attggttttc gggaggttct caaaacccgt tgggaaactc atgtttacgg aaaatattct
     1561 aatgagtccg tataagaata accaaactgc actcatgttt cgattaacga atcgaatgaa
     1621 aagtcagtta gttaatttga gtgcaaatgt gagttcgtct ttgaatttag tggatgaaaa
     1681 tggtgttctg gctagaaggt tctttcgatt gaaactcgaa cgggatgaga ttgtgttttt
     1741 cccaactagc tggacgattg tgcatccgat tgatgagaat agtccgtttt atgggatgac
     1801 cgaagaagag ttccgggcat cagaaccaga attactcata ttaattcaag gatatgacga
     1861 ttcgtatttt gccaatatcc aagtgagata ttcatatatc gaagaaaata ctgtttgggg
     1921 aggaaagtgg agaaagattc ttcaagttgc ggaagatggt tcttcagaaa tccacttaaa
     1981 ccactttaat gatttcgatc gggtggaaat taattcactt attcccgata aaagtatccg
     2041 tgaaatcgcc gattaataac tagcgattca cataaacaaa aaaaccccgg ttgctcaccg
     2101 gggttcaaat cgttaagata agattgctta ttttaaagaa ttcacgtaac gagtgatttt
     2161 acccaccaag ttacctgctt tgttcttatg aataatgctg cgtttcgcta acttatctag
     2221 catagatgaa acatctgcta gttgagcctg cgcttctttc ttatctgaag tcagacgaag
     2281 tttcttcagc gcattacgcg tagtcttgtg aaaataacgg ttacgcagac gctttgcgtc
     2341 gtttgagcgg atccgcttga ttgctgaagg atgatttgcc atttgctttc ttcgctgtta
     2401 atgtagtagc ccgtagggga atcgaacccc tgtaccaaga atgaaaatct tgtatcctaa
     2461 cccctagatg aacgggccat ttttttttca aaaattggaa tgcaaatgta gtccaaaatt
     2521 tgactcagac aagagctgtc aaatactagt tcctaaaaaa tatgcttgtc gactgttttt
     2581 caggcattta attttacaaa tagactaaaa accttgtcta aggacttgtc tattcgcccg
     2641 tgtttatgac attttatggt acatttgcag cgaaatgaat acaaagaaac gtgtagcatt
     2701 ttacaccttg gggtgtaagc tcaatttctc agaaacctca actgttgcac gcttattcca
     2761 aggtgctgga tttgacaagg tagaatttga tgattctccc gatatatatg taatcaatac
     2821 gtgttcggta accgacaatg cggataagaa gtgtaaaagt ttggtgaatc gtgctttgcg
     2881 ggtttctcca catgcgtatg tagctattat aggttgttat gctcaattaa aaccggaaga
     2941 aattgctgca attccaggtg ttgatttggt attaggtgct tctgaaaagt ttaacattct
     3001 cgagcacatg gaagaaatgg agaagaatgt aaaaactcaa atccatgctt gcgaaataga
     3061 tgctgttaac acctttgttc catcttacag ttcaggtgat cgcacacgta cctttttcaa
     3121 aattcaggat ggatgtgatt attcctgctc attttgcact ataccattag cacgaggtaa
     3181 aagtcgaagc gagagtattg cttttacctt ggatgcagca cgaaaactta ttcaagaaac
     3241 gggttcgcgc gaaatcattc taacaggagt aaatacagga gatttcggtc gacataatgg
     3301 tgagcgattc atcgatttaa tcaaagaact cgatttatta gaaggaatag accgttttag
     3361 aatatcctcc atcgaaccta atctgttgag cgatgaagta attgaattcg ttgccaattc
     3421 gaacaaattc gttccgcatt ttcatattcc actacaatcg ggaagcaacg ctactcttag
     3481 aagaatgcgt cgtcggtatc aacgcgatgt gtatgaaagt cgagtagctt taatcaaaca
     3541 gctcatgccc gattgctgca ttggagtaga tgtgattgtt ggatttcctg gggaaacgga
     3601 agaagaattt ctggagagtt acacattcat tcaggaacta gatgtttcgt atctacatgt
     3661 atttacctat tctgaacgtc ccaatacatt agcgattgaa atgcctgatg tagtttctga
     3721 taaggataga gcgaaaagaa gtaaaatgct tcacatacta tcggacaaga aaaagcgata
     3781 cttctatcaa caccaattaa ccaaaacgaa gcaggtactt tgggaaggcg aacgcgctgg
     3841 agattggatg tatggattta ccgaaaatta cgtgaaagtg aaaactcctt atcaacccga
     3901 aaaaacgaat acacttgaat ggatatgcct tcaggatatt gattcggaag gaatttgcac
     3961 agcaacatcc ttaataccag cttaaaccat ctgccagttt taaaacaaaa attatgtacg
     4021 caactattag cgagctttta aaatctttcg gaatcgatat tccgcttccg atacagacat
     4081 ttggtttttt cgttgccatt gccttcatcg tagccgccta tctactttct tctgaattgc
     4141 gaagaaaaga agctcttggt ttattcaaac ctagttttag aaaagtagaa acaggaaaag
     4201 aagctggcac gttcgattac attctcgctg gactcattgg attcctaatc ggtttcaaag
     4261 ccttttacat gatcatgaac tacagcgaat ttgttgacga tccgcaatca acattattat
     4321 ctgggaaagg aaattttata ggtggcttgt tagtcgctgc agccatgatt tacttgcgat
     4381 atcgcgaaag tgagtcacag aaaaaacaaa cacgcgcaac tgttcaaatg cccctaaagg
     4441 ctgaagatca cgtaggaaac atcatattat taggtgttat aggtggctta ttgggtgcta
     4501 aaatttttca caacctcgaa aatccttccg agttcatggc cgatccaatt ggggctttgc
     4561 tttccttttc gggattaact ttttatggag gattaatagt cgcctctatt ctcatcataa
     4621 gatatggtat aaaaaacgga cttccggctc ttcacctcac cgatgctgcc gctcctgctt
     4681 tgatgctgtc ttatggtgtt ggaagaattg gttgcatgat gtcgggtgat ggggattggg
     4741 gaattgtaaa caacaatcct aaaccaggtt ggatgagtgc tttacccgat tggatgtggt
     4801 cgtttaagtt tcctcacaat gtaaataatg aaggaattcc aatcccaggt tgtgtaggaa
     4861 gatggtgtca cgaattgccc gaagctgttt atcctacatc gttttacgaa acactcatgg
     4921 caattgtcct ttttggaatt ctttggatga ttcgtaagcg tatccaaact ccaggaatgc
     4981 tctttagtat atacctaatc ttaaatgggg ctgaacgatt cctgatcgaa tcgattcgag
     5041 taaatacaca gtatcatatc atgggaatgg gcatcaccca agctcaaatc atttcaac
//
LOCUS       DAOOQW010000172         5085 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_19749_length_5085_cov_5.163022, whole genome shotgun sequence.
ACCESSION   DAOOQW010000172 DAOOQW010000000
VERSION     DAOOQW010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5085
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19749_length_5085_cov_5.163022"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(44..1333)
                     /locus_tag="PK281_09110"
     CDS             complement(44..1333)
                     /locus_tag="PK281_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOY49546.1"
                     /translation="MSKSNRIPRQTLVLIVVAALGYFVDIYDLILFNVVKKESLLALG
                     ISDPAQIKNISISLFNWQMGGMLLGGLLWGILGDKKGRLKVLFGSILLYSAANVFNAF
                     VTDIPSYAMVRFIAGIGLAGELGAGITLVSEVMSKENRGYGTMIIVTFGALGAVLAAL
                     VGGEGHAIQDVIHRVSGITLANWQVAYIVGGILGLMLLLLRLGTLESGMFRNLHQQEV
                     GRGQFLKLFSKKETAFRYINCILIGLPVWYAIGILIALAEDFAGTSVLGITGKVVNGQ
                     AVMYAYLGLSAGDLLSGLLSQFWRSRKKVVLLYLGLCATLTLVYLFFSKGKSAEYFYL
                     ICFLVGVATGYWALFVSIAAEQFGTNIRSTVTSTVPNFVRGAVIPITGSFRLLEGSLG
                     TVGSALAVGCVCIGLAAFSMYSLKDTFGKDLDYIETL"
     gene            complement(1336..2172)
                     /locus_tag="PK281_09115"
     CDS             complement(1336..2172)
                     /locus_tag="PK281_09115"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HOY49547.1"
                     /translation="MQHADFSWRSGSDQIKLFGQVWSPIEEPIAVIALVHGMGEHSGR
                     YRRLAEFFTSHHIAVITFDQRGHGRSEGKKGHIVHFNQLLEGIEELVDRANLRFKQKP
                     IFLYGHSMGGNVALNFALKKPNSIKGVIATGPWLRLAFDPPAIQLKLANVMRNLYPGF
                     SQASKLKTEHLSHDKAVVEAYEKDKYVHDRITASFFANLYSSGLYALQHANELKIPAL
                     IMHGEDDKITSQPASAEFAQKANLMSEYKSWPGMYHEIHNEPEKEEVFNHILNWIQRQ
                     LR"
     gene            2308..3384
                     /locus_tag="PK281_09120"
     CDS             2308..3384
                     /locus_tag="PK281_09120"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOY49548.1"
                     /translation="MLRKILRITGYLVLLLVLAFVGLLSFSTLTDYRPEPESEEEVAI
                     EGQAELKNIPDTLSLLIWNIGYCGLGAEMDFFNDGGKTVRASEEQSARYLNAVATFIG
                     TMKDSADVVMIQEVDRNSKRSYNTDQIPVISKQLPGFVSSFALNYDVKFVPVPFSFPY
                     TPYGKTYGGLVSFSHLPATRATRVQYPGGFDWPTRLYMLDRCALELRYPLKDGKELII
                     VNTHNTAYDATGEIKKVEMAFMKKRYEAEVAKGNRVIIGGDWNQVPPGFDSKHFSNNM
                     SEGYTPQSLSVDMLPKDFVVSYDSSTATNRSNVTPYVADSTYTTLIDFFLSSPGVEVL
                     DVHAIDMQFRYSDHQPVLLKVAIH"
     gene            3460..4002
                     /locus_tag="PK281_09125"
     CDS             3460..4002
                     /locus_tag="PK281_09125"
                     /inference="COORDINATES: protein motif:HMM:NF012479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HOY49549.1"
                     /translation="MKLSRKSDKGMIALKPKSAAANKPPADLKLTLSNGKEEALNSYL
                     GKKVLLVNTASDCGYTGQFAELQKLHELYGIKLQIIGFPSNDFKEQEKADDKAISEFC
                     QVNFGVTFPLAKKSVVLKNVGQNPVFRWLSNPSENGWNSQEPVWNFSKYLLNEQGELI
                     ASFGPAISPEDAEVKKLIES"
     gene            complement(3999..>5085)
                     /locus_tag="PK281_09130"
     CDS             complement(3999..>5085)
                     /locus_tag="PK281_09130"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HOY49550.1"
                     /translation="TIHFLTTTRPNVVLVVFEGWAADVIEALGGYSGVAPHFSKLIQQ
                     GVSFDSCYASGNLSDQGMAAVFSAFPAQPRSSIISVPNKYVHLPCINTEFKKNGYATS
                     FLFGGQLSYGNIRSYMYYNGFDRIQEEKDFASSVYRGRLGVHDGDLYDREIQELKSEK
                     QPFFASMFTLSTHGPYDIPVPDVLNWGEKENGYINTVHYADSCLNHFIEQARQQPWYD
                     NTLFVFVSDHHHNTPKSYSYFSPEYRRIPLVFYGNVIKPEFRGMKYHAICSQLDLAST
                     LLHQLNMDASPFVWSKNLFNPYTPQLAVYAFDEGFGIIRPQEHLVYHIHENRFEFEKY
                     LSASSKPRLTREGKAYLQRVTEEFWKY"
BASE COUNT         1552 a         1023 c         1062 g         1448 t
ORIGIN      
        1 gtttcttgca agacttatct ggacaagaac ttatcagctc cgctcagagc gtttcaatgt
       61 aatcgaggtc ctttccaaag gtatctttca aggagtacat cgagaaagcg gccagtccga
      121 tacatacgca gccaactgca agagcactcc caacggttcc taaactacct tccaacaaac
      181 ggaaacttcc ggttattgga attactgcac ctctcacgaa attaggtacg gttgaagtca
      241 ctgtacttcg aatgttggtt ccaaattgtt cggctgcaat gcttacaaac aatgcccagt
      301 agcctgttgc tactccaact aagaagcaaa tcaggtagaa atactcggct gattttcctt
      361 tggagaaaaa tagatagacg agtgttagcg tagcacataa gcctaaatac aggagcacta
      421 cttttttccg gctacgccaa aactgactaa gcaaaccact taacaaatca ccagctgaaa
      481 gtcctaaata ggcatacata actgcttgtc cgtttaccac tttacccgta attccaagca
      541 cactagtacc ggcaaaatcc tccgctaggg caattaaaat tccgatagca taccaaacag
      601 gtaagccaat gagaatgcag tttatatagc ggaaagctgt ttctttttta gaaaagagtt
      661 tgaggaattg tcctctgcca acttcttgtt gatgcaggtt tcggaacata cccgattcga
      721 gtgtgccaag gcgaagtaga agtagcatca agcctaaaat tccacccaca atgtatgcta
      781 cttgccagtt ggcgagtgtg attcctgaaa ctcgatgtat cacatcttga atggcgtgtc
      841 cttcaccacc aactaatgct gctaaaacag cacccagtgc accaaacgtt acaattatca
      901 tggttccata accgcgattt tccttgctca taacttccga aacgagcgta attccagcac
      961 ctaattcacc cgctaatccg attccagcaa taaagcgaac catagcatac gaaggaatat
     1021 cggttacgaa tgcattgaaa acattagctg ccgaatacag taatatggaa ccgaataaaa
     1081 cttttaatct gcctttttta tcccctaaaa ttccccaaag caaaccaccc aagagcattc
     1141 cgcccatttg ccagttaaat agggaaatac tgatgttttt gatttgagca ggatcgctaa
     1201 ttccgagtgc aagaagactt tccttcttaa caacgttaaa gagaattaga tcgtagatat
     1261 cgacaaaata tcccagagca gcaacgacaa tcaggacaag cgtttgccga ggaatacggt
     1321 tggatttaga cattgttaac gtaactgacg ttgaatccaa tttaagatgt gattgaacac
     1381 ttcttctttt tcgggttcat tatgaatttc gtggtacatg cccggccaag atttgtattc
     1441 gctcatgaga ttggcttttt gagcaaattc agcggaagca ggttgagagg taattttatc
     1501 gtcctcacca tgcataatca aagcaggaat ctttaattcg tttgcatgtt gtaaagcgta
     1561 aagtcccgat gaataaaggt ttgcaaagaa agaggcagta attctatcgt gaacatactt
     1621 atctttttca tacgcttcaa ctacagcttt atcatgagaa agatgttcgg ttttaagttt
     1681 tgacgcttgt gaaaatccgg gatacagatt acgcatcaca ttcgccaatt ttaattgaat
     1741 ggcaggagga tcaaaagcaa gtctaagcca tggaccagtt gctatcacac ctttaataga
     1801 attcggtttt ttaagggcga aattgagtgc aacatttcct cccatgctgt gtccataaag
     1861 gaagataggt ttctgtttga aacgcaaatt cgcacggtca accaattctt caatcccttc
     1921 caataattgg ttgaagtgaa cgatatgtcc ttttttacct tctgaacgac cgtgtccgcg
     1981 ttgatcaaac gtgataactg caatatgatg cgaggtgaag aattcggcaa gtcgacggta
     2041 acgaccgcta tgttcaccca ttccatgaac aagagctatg acggcaattg gttcttcgat
     2101 tggcgaccaa acttggccga ataatttaat ttgatcggat ccagatctcc agctaaaatc
     2161 ggcgtgttgc atggttaaaa tgtgaactgt aaaaatagaa atgttcctat tggaattacc
     2221 tattcgttaa ttctatcctt caagaattgc aagtccattt aagagcgtca tattttgtag
     2281 gtttgacaca cgtttcaaat ccaacctatg cttcgaaaaa tcctacgtat aacgggctat
     2341 ctagttttat tgctggttct agcatttgtc ggattgctca gtttttcaac cttaaccgat
     2401 taccgacctg aacctgaaag tgaagaagaa gtagcaattg aaggacaagc agagctgaaa
     2461 aatattcccg ataccttgag tttgctcatt tggaatattg ggtattgtgg tttgggagct
     2521 gaaatggatt ttttcaatga tggaggaaaa acagttcgtg caagtgagga acaatcggct
     2581 cgttacctaa atgccgtggc aacttttata ggtacaatga aagatagtgc cgatgtggtg
     2641 atgattcaag aagttgatcg gaacagcaaa cgcagctaca ataccgatca aatccccgtt
     2701 atctctaagc aactgcctgg tttcgtatca agttttgctt tgaattatga tgtgaaattt
     2761 gtccctgttc catttagttt tccttacact ccttacggaa aaacatacgg tggattggtg
     2821 agcttctctc atcttcccgc cacgcgtgca actcgagttc aatatcccgg tggatttgat
     2881 tggcctacac gtttgtatat gctcgatcga tgtgctttag aattgcgcta tcctttaaaa
     2941 gatgggaaag agcttataat cgtgaacacg cacaacactg cttatgacgc tacaggtgaa
     3001 attaagaaag tggaaatggc atttatgaag aaacgctacg aagcagaagt ggcgaaagga
     3061 aatagagtca tcattggagg tgattggaat caagttcctc ccggttttga ctcgaaacat
     3121 tttagtaaca atatgtcgga aggatatact ccacaatcgt tgagtgttga tatgcttcca
     3181 aaggattttg tggtgagtta tgattccagc acagcaacta atcgtagcaa tgtaactcca
     3241 tacgttgcag atagtacgta tacaacattg attgatttct tcttgagttc acctggtgtt
     3301 gaggtccttg atgtgcatgc gatcgatatg cagtttcgct atagcgacca ccaacctgtt
     3361 ttgctcaaag tggcaataca ttaaaataaa acccaacttc atttctctta taaaatgact
     3421 attcgtcaac gaatactgaa atcgatatat ccgcttgtca tgaaactttc acgtaaaagt
     3481 gataagggaa tgattgcgct aaaacctaag tcggctgccg cgaataaacc acctgctgat
     3541 ttaaaactga cgctttcaaa cggaaaagaa gaagcactta attcctacct gggaaagaaa
     3601 gtgttattgg tcaataccgc ttccgattgt ggatacacgg gacagtttgc agagttgcag
     3661 aaattgcatg agttatatgg catcaaattg caaatcattg gatttccttc gaacgatttc
     3721 aaagagcagg aaaaggccga tgataaagcg atttcggaat tttgtcaggt gaattttgga
     3781 gtgacttttc cgttggctaa gaagtcggta gtactcaaaa atgttggaca gaatcctgta
     3841 tttcgttggt tgagtaatcc atccgaaaat ggttggaatt cgcaagaacc tgtttggaat
     3901 ttcagcaagt atttattgaa tgagcaggga gaattgattg ccagttttgg tccggctata
     3961 tctccagaag atgcagaagt taagaagctg attgaatctt aatacttcca aaattcttct
     4021 gtaacacgtt gcaggtaagc ttttccttcc cgtgtcaatc gaggtttact ggatgcagaa
     4081 aggtatttct caaattcaaa tcgattctcg tgaatatgat aaacgaggtg ttcctgaggt
     4141 cggataattc caaatccttc atcaaaagcg tatacagcta actgaggagt ataaggattg
     4201 aagagatttt tactccacac gaaaggactt gcatccatat ttaattggtg aagtagcgta
     4261 gaggccaagt cgagctgcga acaaatcgca tgatatttca ttccccggaa ctcaggttta
     4321 atcacatttc catagaaaac aagcggtatt cgtcggtatt cgggcgaaaa gtaagagtag
     4381 cttttaggtg tattgtgatg atgatccgac acgaatacaa ataatgtatt gtcgtaccaa
     4441 ggttgctgac gagcttgttc gatgaagtga ttcaaacaac tatcggcata gtgaacggta
     4501 ttgatgtatc cgttctcttt ttcaccccaa ttcaacacat ctggaacggg aatatcgtat
     4561 ggcccgtgcg tgctgagtgt gaacatagaa gcgaaaaatg gttgtttttc acttttgagt
     4621 tcttgaattt cacgatcata caaatcacca tcatgaacac ctaatcgacc tcgataaaca
     4681 gaagaagcaa aatctttttc ttcttgtatc cggtcaaaac cgttgtaata catataggag
     4741 cgaatgtttc catagctaag ctgtccacca aaaaggaaag aagtagcata tccgttcttt
     4801 ttgaattcgg tattgatgca gggcagatga acgtatttat tgggaacaga aatgatggaa
     4861 gatcttggtt gagctggaaa ggctgagaat acagctgcca ttccttggtc gctcaggtta
     4921 ccggatgcat aacaggaatc gaatgaaact ccttgttgga taagcttgga aaaatgaggt
     4981 gcaacacccg aatatcctcc cagtgcttca attacatcgg ccgcccatcc ttcaaaaacc
     5041 actaaaacaa catttggacg agttgtcgtt aagaaatgaa tagta
//
LOCUS       DAOOQW010000173         5066 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_19842_length_5066_cov_4.036520, whole genome shotgun sequence.
ACCESSION   DAOOQW010000173 DAOOQW010000000
VERSION     DAOOQW010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5066
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19842_length_5066_cov_4.036520"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /locus_tag="PK281_09135"
     CDS             <1..597
                     /locus_tag="PK281_09135"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY49551.1"
                     /translation="GTDTAFTVIVRDTLDASLDVPSFRFEGSSHPMTYTISGEGIVTF
                     VFANILLPDSFVNEAGSHGVLSYFIKRKMDIPFGTEINNTGYIYFDFNPPIQTNTTRN
                     TLFDPTLGMKLVGNMQFNMQPNPTLDRSRITLQLNQADDVQVSVLDVSGRLIQYRKLG
                     RLAAGEHQFQTESLPAGTYLVRIQAGVNVQTKRLIVTR"
     gene            682..1962
                     /locus_tag="PK281_09140"
     CDS             682..1962
                     /locus_tag="PK281_09140"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOY49552.1"
                     /translation="MSFLLVLHKRLNVSCTVFCLANDRVEALTDFQIDWLDSPPESSS
                     TGWKTSFYFEQGYIQALIQGSGAKGRFLFPCIRQGNEIIAWGCFQELILYREELDELG
                     RLFASDSRFAIKVEGLVKSLIHLGSGKKGIRILIAGNCQVTGPYGMTFSPHLSESVKA
                     ECWSNILKQAEQQFGPYSITLVKDFNDNQDLIIDSLRTDGYRRVPTLPVMRFHLDKSW
                     NQYDDYLNAMASKYRIRAKAARKKGVKLQREIWTEVEIKEHLEQINQLYRNVFDKARF
                     RLFRISPNYFLHLKQAFQERFLFKAYLFDGKLVGFSTFLIDPVQSDAHLIGIDYSANK
                     QFSLYQNMLYDYVDTAIEHQTACIDFGRTAMEIKSTIGAIPEDSPVIVKMRNPLLNGM
                     ACILVENSTPAPWIQRHPFREEEHLTLAESSDSN"
     gene            2143..2808
                     /locus_tag="PK281_09145"
     CDS             2143..2808
                     /locus_tag="PK281_09145"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOY49553.1"
                     /translation="MAQKEKTGEITLLLADRIPLFSSGLQHMLMSGRGFSRVDVTDNR
                     KEVLQYLQAYRYDILMLDTRLNDSESIEILQHIIRLYPNQKIIFMGRLRDNRYLRDCI
                     QNGLKGFVLRSSSLNEVLLAIRIVYQGGEYFSPEVARVIYKIVANPVAAEDGYTHMLM
                     SRELQVLRRICDEKCNDQIAAELNISLSMVKKYRSSLLKKTNSKNTVGLIRYAFQQGI
                     LKL"
     gene            complement(3089..3709)
                     /gene="folE"
                     /locus_tag="PK281_09150"
     CDS             complement(3089..3709)
                     /gene="folE"
                     /locus_tag="PK281_09150"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016987977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HOY49554.1"
                     /translation="MEETEFQPEGYQKIDRYNTEKTDELSSHYSTIISQLGEDPEREG
                     LKKTPERVAKALQFLTHGYDLDPAEILKSAMFAEDYKQMVIVKDIELYSLCEHHMLPF
                     FGKAHIAYIPNGHIVGLSKIPRVVDAFARRLQVQERLTTQIRDCIEETLRPLGVAVVI
                     EAQHLCMQMRGVQKQNSVTTTSAFTGEFMVEKTRAEFFSLIGHRLH"
     gene            complement(3715..4128)
                     /locus_tag="PK281_09155"
     CDS             complement(3715..4128)
                     /locus_tag="PK281_09155"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HOY49555.1"
                     /translation="MIYITRKEHFNAAHKLARPEWSDEKNVEVFGKCANVNWHGHNFN
                     LHVTVKGVPSEVTGFVIDLKVLSALIRERVTEKLDHKNLNLDVDFMKGIMPSTENLAM
                     KIWDQLEKPILSHGCALHSVKLFETENNYVEYLGA"
     gene            4464..>5066
                     /locus_tag="PK281_09160"
     CDS             4464..>5066
                     /locus_tag="PK281_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007651429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOY49556.1"
                     /translation="MITVDNLTVEYSGKTLFSGISFAINPTDKIALMGKNGAGKSTIM
                     KIIAGESNATRGAVRAPKEAVIAYLPQHLLTEDNATVFDEAAKAFSHVFAMRDEMEFL
                     NKELETRTDYESKEYMQIIERVSDLSEKYYALEEVNYDAEVEKALKGLGFKQEDLYRP
                     TSEFSGGWRMRIELAKILLKKPDLILLDEPTNHIDIESVMW"
BASE COUNT         1443 a          992 c          995 g         1636 t
ORIGIN      
        1 ggaaccgaca cagcttttac agtaattgtt cgtgatacat tggatgcatc tttggatgtt
       61 cctagtttcc gtttcgaagg aagtagccac ccaatgactt atactattag cggagaaggc
      121 attgtaacct ttgtttttgc taatatctta ttgcctgata gttttgtaaa cgaggcagga
      181 agtcatggtg tgttgagtta tttcatcaaa cgtaaaatgg acattccatt tggtacagag
      241 atcaacaata caggatatat ctattttgat ttcaatcctc cgattcagac gaatacaact
      301 cgtaacactt tattcgaccc aactttggga atgaagctcg tcggaaatat gcaattcaat
      361 atgcagccga atccaacgtt agatcgtagt cgcatcacgc ttcagttgaa tcaagcagac
      421 gatgttcaag tttcggtttt agatgtttca ggacgtttga ttcaataccg taagcttggt
      481 agattggctg caggtgagca tcaattccaa acagaatctt tacctgccgg aacttattta
      541 gttcgcattc aagctggcgt aaatgttcaa actaaacgat tgatagtaac tcgttaagct
      601 caataattca tttctaaatc gcccgattgg ttttctagtc gggcgatttt ttttgccaac
      661 aatttagctt tgctttcgtt tatgtcgttc ctacttgttt tacacaagag gcttaatgta
      721 tcttgcactg tgttttgttt agcgaatgat cgggtggaag cccttactga ttttcaaatt
      781 gactggttag attctccacc tgaatcaagt tcgacaggtt ggaaaaccag tttctatttt
      841 gagcaaggtt acattcaagc acttattcaa ggttcaggtg caaaagggcg atttttattt
      901 ccatgtattc gtcagggaaa tgaaataatt gcttggggct gctttcaaga attgatttta
      961 taccgagaag aactcgatga attaggacgc ttattcgcat ccgatagtcg ctttgccatt
     1021 aaggtagaag gcttagttaa atcacttata catctcggaa gtggtaagaa aggtattcgc
     1081 attctgatag ctggaaattg tcaggttaca ggcccatacg gaatgacttt ttcacctcat
     1141 ttgagtgaat cggtaaaggc ggaatgttgg tcaaacatat tgaagcaggc agagcaacaa
     1201 ttcggtccat attccatcac attggtgaag gattttaatg ataatcagga tttgattatt
     1261 gattcattac gtaccgatgg atatagacga gtaccaactt tgccggttat gcgatttcat
     1321 ctggataaat cttggaatca atacgatgat tacctgaatg cgatggcatc gaagtatcgt
     1381 attcgtgcca aagcagcccg taaaaaaggt gttaaattac aacgtgaaat ctggactgaa
     1441 gtcgagatta aagagcattt ggaacaaata aatcagcttt atcggaatgt ttttgacaag
     1501 gcacgttttc gattatttcg gatatctccc aattactttc tgcatttaaa acaagcattt
     1561 caagagcgtt tcttatttaa agcatattta tttgatggta aacttgttgg gtttagcacc
     1621 tttctaattg atccagttca aagtgatgca cacttaatcg gtattgacta ttcagctaat
     1681 aagcagtttt ccctctatca aaatatgttg tatgattatg tcgatactgc catcgaacat
     1741 caaacagcct gtattgattt tggccgaact gctatggaaa taaaaagcac tattggtgca
     1801 ataccagaag acagtcccgt gattgttaaa atgcgcaatc cacttcttaa tggaatggct
     1861 tgtattctgg tcgaaaattc tactccagca ccttggattc aacgtcatcc ttttcgagaa
     1921 gaagagcatc tcactttagc tgaatcttca gattctaatt gaagcctaat tggcggaatt
     1981 tgatctgttt ttttaccgaa attcaaattt tgtatattga ttcttaagag tttgtttgac
     2041 aaattctctt atacacatgt tgtatttatt atgacgtaat aggtgtattt gtcaagctac
     2101 ttgtacattc attttgccgg ggttttatca aattgcttgt atatggctca aaaagaaaaa
     2161 acgggtgaaa taaccctgtt attggccgat cgtattccgc tttttagcag tggtttgcaa
     2221 cacatgctta tgtctggaag aggtttttct cgggtcgatg tgaccgataa tcggaaagaa
     2281 gtacttcaat atttgcaggc ttatcgttac gatattctca tgttagatac gcgattgaat
     2341 gattcggaaa gtattgagat tttgcagcat atcattcgac tttatcctaa ccaaaagatc
     2401 attttcatgg gtcgtttgcg cgacaatcgt tatttgcgcg attgtatcca aaatggtttg
     2461 aaaggctttg tattacgttc gagtagtttg aacgaagttt tattggcaat acgaatcgtg
     2521 tatcaaggtg gagagtattt ttcacccgaa gtagcccgtg tgatttataa aatcgtagca
     2581 aatccagttg ccgcagaaga tggttataca catatgctca tgtcgcgaga acttcaagta
     2641 cttcgtcgga tttgcgatga aaaatgcaat gatcaaattg ctgccgaact caatatcagt
     2701 ctttctatgg tgaagaaata ccgaagttct ttgctaaaga aaacgaattc aaagaatacc
     2761 gtcggactca tccgatacgc ttttcaacaa ggtattctca aactttaacg ggattgctga
     2821 cgagtaattc agttatgtag ttgaaacagt ataatattta ttgaataagg taaaatcctc
     2881 ctgtactaaa gttaccggaa tttataacca atctaccact tgaccactct attggaatag
     2941 tacatctttg aaacataatc ctattaagat tgctttatga gtatgtatta agtgtatgta
     3001 ttaggtccaa ttaggacgat tatcacgtgg atggatggcc cacgaaatga gttcccgatt
     3061 cagctatatg ccggatcggg aatttcattt aatgcaatcg atgtccgatt aaactgaaaa
     3121 attcagcacg tgttttttct accatgaatt cacccgtaaa tgcagatgtt gttgtaactg
     3181 aattttgttt ctgcacacca cgcatctgca tacataagtg ttgagcttca attactacag
     3241 caactccaag cggacgaagt gtttcttcta tgcaatcacg aatttgcgtt gttaatcgtt
     3301 cctgcacctg taaacgacgc gcgaaagcat ctaccacacg tgggatttta cttaatccaa
     3361 ctatatgtcc gttagggata tacgcaatat gcgcctttcc aaaaaatggc aacatgtgat
     3421 gttcacacaa ggagtataat tcaatgtcct tgacaatcac catttgcttg taatcttcag
     3481 caaacatggc cgatttcaag atttcggctg gatccaggtc ataaccatgt gtgagaaact
     3541 gtagcgcttt agcgacacgt tcaggagttt ttttcaatcc ttcccgctca ggatcttccc
     3601 ctaattgtga gatgatagtc gaatagtgac tcgataactc atcggtcttt tccgtgttat
     3661 agcgatcgat cttttggtaa ccttcgggtt gaaattcggt ttcttccatc acatttatgc
     3721 gcctaaatac tcgacgtaat tgttttcggt ttcaaataat ttaacactat gcaaagcaca
     3781 tccatgagat aggataggct tttcgagttg atcccaaatc ttcatcgcca aattctcggt
     3841 cgatggcatg attcctttca tgaaatccac atccagattt aggtttttat gatccagttt
     3901 ttctgtcacg cgttcgcgga ttaatgcact caaaactttc aaatcaatca caaatccagt
     3961 aacctcacta ggcactcctt ttaccgttac gtgcagattg aaattatgtc catgccagtt
     4021 gacattcgca catttgccaa atacttccac atttttttca tccgaccatt caggccttgc
     4081 tagcttatgt gccgcattaa aatgttcttt tcgggtaata taaatcattc ggttgggtgt
     4141 aggccgcaaa gatagagatt agattttacc agcgcttcgc tcggcgatat acgatgatcg
     4201 atatacgatg tacgatatac gatgtacgat tgcttcgagg aagattgatt taacgtacat
     4261 atcaataagc ttccttttca attcttagta ctctaaactt tccactttct acttttctac
     4321 tttccacttc ctactctaaa ctttccactt tccactttcc acttcccact tctcactttc
     4381 cacttccctc tttctccttt cccctttcta cttttttact ttccactccc tatttgttta
     4441 cttttgccgc ccatctttcc aatatgatca ctgtcgacaa tctaaccgta gaatactcgg
     4501 gtaaaacctt gttcagcggt atttcctttg ccatcaaccc aaccgataaa attgccctga
     4561 tgggtaaaaa cggagcaggg aaatcaacca tcatgaaaat tattgctggt gaatcaaatg
     4621 ctactcgtgg tgcagttcgg gcgcctaaag aagccgtaat tgcttatcta cctcagcatt
     4681 tgttgaccga agataatgct actgttttcg acgaggctgc taaggcattt agtcatgtgt
     4741 ttgctatgcg agacgagatg gaatttctca ataaagaact cgaaacgcgt accgattacg
     4801 agagcaaaga atacatgcaa atcatcgaac gggtgtcgga tttgtcggag aaatactacg
     4861 cgcttgaaga ggttaactac gatgccgaag tagagaaagc attgaaagga cttgggttta
     4921 agcaagaaga tttatatcgc ccaacgagtg agtttagcgg tggttggaga atgcgtatcg
     4981 aattggcgaa aatcctcctc aaaaaacctg atttaatttt gttggatgag cctacgaatc
     5041 acatcgacat tgaatcggtg atgtgg
//
LOCUS       DAOOQW010000174         5039 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_19969_length_5039_cov_3.878210, whole genome shotgun sequence.
ACCESSION   DAOOQW010000174 DAOOQW010000000
VERSION     DAOOQW010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5039
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19969_length_5039_cov_3.878210"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            145..2568
                     /locus_tag="PK281_09165"
     CDS             145..2568
                     /locus_tag="PK281_09165"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase
                     metal-binding domain-containing protein"
                     /protein_id="HOY49557.1"
                     /translation="MLSKAKVPEIKTDLHCYHCGETCKENTTWIEDKAFCCDGCRLVF
                     EILNENNLCSYYTLNPSSGNSPDKANYTGKFAYLDQEEVSSKLLHFTNDKQSHITFFV
                     PGIHCSSCIWLLENLQRLEKGVISSSVNFPKREVTVIFQQAETSLSKIAERMTMIGYE
                     PHISLNDIEDKHATKADRSQWYKIGIAGFCFGNIMMMSFPEYFSIGEVAGNADLKLTF
                     SWLNFALSLPVLFYSSRDFFISGIGAIRSKHLNIDLPIAAAVLMTFLRSVYELVSGTG
                     PGYFDSMTGIVFFMLIGRAFQNRTFETLAFDRDYKSYFPVAVTVISESGEEQIPVTRI
                     KKGMRMLIRNQEIVPADAILLRGEAKVDYSFVTGESDPVIRKKGDLVYAGGRQSGTSI
                     ELEIVKPVKQSYLTQLWNSPAFRKDQAEEEKASMENKINQYFTLGIFIVALASASYWL
                     WAGETHRMMNAVTTILIVACPCILLLASTFANGNVLRILGRNGFFLKNAFVIEKMAKA
                     DTIVFDKTGTITLSRDAEVSFQGRKLSKDEITAVYSLVLQSTHPLSSSIAASYQGNER
                     LVVTDFSEFSGKGIQGTVNEMMVQVGSASFTNQTVSDEKLNTTRIYLRINGESCGYYT
                     LQHQLRDQLPTLLSRLRRNYDMYLLSGDTDSDRQQLSSWFEPNQMHFQQSPEDKLNFI
                     RKLQDEGKTVLMLGDGLNDAGALQQSDAGIAVSDNLNNFSPACDAILDGKILSRLNTL
                     LDYCKSGKRVIAVSFAISLLYNCVGLWFAVRGELQPVIAAILMPVTSISIVSIATLSS
                     TLIARKKNL"
     gene            2688..2879
                     /gene="ccoS"
                     /locus_tag="PK281_09170"
     CDS             2688..2879
                     /gene="ccoS"
                     /locus_tag="PK281_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HOY49558.1"
                     /translation="MSALYVLIFISICVALAFLAAFLWSVKKGQFDDSYTPSVRMLFD
                     DEAIEPKKETPSTQPIPKH"
     gene            2887..>5039
                     /gene="ccoN"
                     /locus_tag="PK281_09175"
     CDS             2887..>5039
                     /gene="ccoN"
                     /locus_tag="PK281_09175"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007649828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HOY49559.1"
                     /translation="MELERFEYDNRTPRNFAVASIIFGLIGMLVGLYIALELVFPNLN
                     LGFPLTTFGRLRPLHTNAVIFAFVGNGIFAGVYYSLQRLLKARMFSNALSNIHFWGWQ
                     LIILAAALTLPLGYTTGKEYAELEWPIDIAIALIWVVFGINMFGTIVKRRVQHLYVAI
                     WFYIATFVTVAVLHIVNSMELPVSFLKSYSAYAGVQDALVQWWYGHNAVAFFLTTPYL
                     GLMYYFVPKAAGRPVYSYRLSIIHFWALIFLYIWAGPHHLLYTALPDWAQSLGVVFSF
                     MLIAPSWGGMINGLLTLRGAWDKVREDVVLKFMVVAITAYGMSTFEGPMLSLKNVNAI
                     AHFTDWIIAHVHVGALGWNGMLTFGMIYWLFPRFYGRELFSKKLANFHFWIGTLGIMF
                     YAIPLYWAGLTQFEMWSEFTADGFLRYPNFLETVTQLIPMYALRSLGGTLYLVGFLVM
                     IYNLWKTAFAGKFIANEAAEAAPLPKGATPGHQASEGWHRVIERKPFQMLLFSLIMIL
                     IGGLVEMIPTFLIKSNIPTIASVQPYTPLELQGRDIYIREGCYTCHSQMIRPFRFETE
                     RYGEYSKAGEYVYDHPFQWGSKRTGPDLQREGGKYPDSWHYNHMDDPRTMSPGSIMPM
                     YPWLLDDDLDTTSTAPKIRAMMTLGVPYPEGYDKIANQDLVRQQNKIVNNLKESGIEA
                     LPNKEIIAMISYLQRLGTDIKGENKIVFGQIATTKP"
BASE COUNT         1457 a         1039 c         1045 g         1498 t
ORIGIN      
        1 cggttgcacc gacaatctaa tgagggaaaa ggaaattctc ttcccaaaaa tgatagagct
       61 ggaacgcgaa gttatgaagc aaaccagcaa tgcacttgaa ggtagaagta ccttaagtaa
      121 cgacgattaa cataagtacc caacatgtta tccaaagcca aagtacccga aataaaaaca
      181 gatctccact gttaccactg cggagaaact tgtaaagaaa acaccacttg gatagaagat
      241 aaagcgtttt gttgcgatgg ctgccgtttg gtttttgaga tactaaacga aaacaacctt
      301 tgcagctact atacactcaa tccttcctct ggaaattcgc ctgataaagc caattacaca
      361 ggcaaattcg cctacctcga tcaggaagaa gtaagtagca aactgctaca ttttacaaac
      421 gacaaacaat ctcatatcac ctttttcgtc cctggaatcc attgcagctc gtgtatttgg
      481 ttactggaaa acttgcaacg tttagaaaaa ggtgtgatta gttcttcggt caacttccct
      541 aaacgtgagg taacagttat cttccaacaa gctgaaacga gtttgagcaa aattgccgaa
      601 cgcatgacca tgattggtta tgagccgcat atcagcttaa acgatattga agacaaacac
      661 gccacaaaag ctgatcggag tcaatggtat aaaatcggaa ttgctggttt ttgttttggg
      721 aacatcatga tgatgagctt cccggaatat ttctccattg gtgaagtagc tggaaatgca
      781 gatttgaaac tcacttttag ctggctcaat ttcgcacttt ctctacccgt gttgttttat
      841 agcagtcgcg attttttcat ttcgggtatt ggagccattc gtagcaaaca cctcaacatc
      901 gatttaccca ttgccgcagc agtgttaatg accttcctga gaagcgttta tgaacttgtg
      961 agtggaaccg gtccgggata cttcgattcc atgacaggca ttgtgttttt tatgctgatt
     1021 ggacgagcgt tccaaaatag aaccttcgaa acattagcat ttgatcgtga ttataaatcg
     1081 tatttccctg tagctgtaac agtaatttcc gaatcaggtg aagaacaaat acccgtaacc
     1141 cgaattaaaa agggaatgcg catgttgatt cgcaatcagg aaattgttcc tgcagatgcg
     1201 attctacttc gtggtgaagc taaagttgat tacagtttcg taactggtga atcagatcct
     1261 gttatccgta aaaaaggaga tttagtttat gctggtggtc gacaatcagg tacgagcatc
     1321 gagcttgaga tagtgaaacc cgttaaacaa agctacctca cacaattgtg gaatagtccg
     1381 gctttccgaa aagaccaagc cgaagaagag aaagcatcca tggaaaacaa aatcaaccag
     1441 tattttacgc tggggatttt tatcgtggca ttggcttctg cttcctactg gctttgggca
     1501 ggtgaaacgc atcgcatgat gaatgcggta accaccattt tgattgtggc ttgtccgtgt
     1561 attttactcc tcgcttccac atttgcaaat ggaaacgtac tccgcatctt aggtcgaaac
     1621 ggattcttcc tcaagaatgc atttgtgatt gagaagatgg cgaaagccga tacgattgtc
     1681 tttgataaaa ctgggaccat cactctaagt agagatgctg aagttagttt ccaaggaaga
     1741 aagctatcga aagatgaaat cactgctgtg tattccttag tgctgcaatc aacgcatcct
     1801 ttaagtagca gcatagcagc atcgtatcaa ggtaacgaac gtttggtagt aaccgatttc
     1861 agtgaatttt cgggtaaagg aatccaagga acggtaaatg aaatgatggt tcaagttgga
     1921 tcggcatcat ttaccaatca aaccgtatcg gatgaaaagc ttaatacgac tcggatttac
     1981 ttacgtatta atggtgaatc gtgtggttat tatacccttc aacatcagct acgcgatcaa
     2041 cttcctacat tattaagtcg cttacgtcgc aactacgaca tgtatttgtt gagtggtgac
     2101 accgattcgg atcgtcaaca attaagttct tggtttgagc cgaatcaaat gcactttcaa
     2161 caatctccag aagacaaact gaacttcatc cgcaagcttc aagatgaggg taagacagtt
     2221 ttgatgttgg gtgatggatt gaacgatgcc ggtgctttac aacaaagtga tgcaggaatt
     2281 gcggtttcgg ataatctgaa caacttttct ccagcctgcg atgcgatttt ggatgggaag
     2341 attttatctc gactaaatac gcttttggat tattgtaaaa gtggtaaacg agtaatcgct
     2401 gtaagctttg ccatctcgct attatataac tgtgtaggat tatggttcgc cgttcgaggt
     2461 gagctacaac ccgtaattgc cgccatactt atgcctgtaa catccataag tattgtctca
     2521 attgctacac tcagctcaac tcttatagct agaaagaaaa atctatgagt gtaaaaaact
     2581 aaataaaacc tgactaaaat catattccta cctgactcgc atcatttaac accttttaac
     2641 acaggtctat ttttgacatc attagaattc ccaattagct cctccttatg agcgcactct
     2701 atgtactaat ttttatcagc atttgcgtag cacttgcctt tttagcagcc ttcctttggt
     2761 cggtgaaaaa aggtcagttc gacgacagtt acacaccatc tgtgcgaatg ctatttgatg
     2821 atgaagccat agagcctaag aaagaaacac cttcaaccca accaatccct aaacattgac
     2881 gttgccatgg aactagaacg ttttgagtac gacaaccgaa ccccgcgaaa cttcgctgta
     2941 gcatccataa tcttcggatt aattggaatg cttgtaggac tttacatcgc gctggagctc
     3001 gtattcccga accttaacct gggttttccc ttaactacat ttggccgact tagaccgctg
     3061 catacgaatg cggtaatttt cgcttttgta ggaaacggaa tatttgcagg tgtttactat
     3121 tcacttcaaa ggcttttaaa agcccgaatg tttagtaatg cattaagtaa cattcacttc
     3181 tggggatggc aattaatcat tcttgcagct gctcttactt taccattggg ttacacaact
     3241 ggtaaggaat atgccgaact cgaatggcca atcgatattg caattgcatt aatctgggtg
     3301 gttttcggga ttaacatgtt cggaaccatt gttaaacgcc gggtgcaaca cttatatgta
     3361 gcaatttggt tctacatcgc aaccttcgta acagtagcgg tattacacat tgttaactcc
     3421 atggaacttc ctgtaagttt cttgaaaagc tacagtgcat acgcaggtgt tcaggatgct
     3481 ttggttcagt ggtggtatgg tcacaatgcg gtggcatttt tccttactac tccttactta
     3541 ggtttaatgt actatttcgt accaaaagca gctggtcgtc cggtttactc ttaccgtcta
     3601 tcgattatcc acttctgggc actaattttc ttatatatct gggctggtcc tcaccacttg
     3661 ttatatacag cacttcctga ttgggctcaa tcattagggg tagtattctc ctttatgttg
     3721 atcgctccaa gttggggtgg tatgattaac ggattattga cacttcgcgg tgcttgggat
     3781 aaagttcgtg aagatgtggt attgaaattc atggtagtgg ctatcactgc ttatggtatg
     3841 tcgacgtttg aaggaccaat gctttcgttg aaaaacgtaa atgcgattgc tcactttacc
     3901 gattggatta tagcacacgt tcacgtgggg gctttaggat ggaacggtat gttaaccttt
     3961 ggtatgatat actggttatt cccacgcttc tatggccgtg aacttttctc taaaaaacta
     4021 gccaacttcc atttctggat tggcacactt ggaatcatgt tctatgcgat tccactttat
     4081 tgggctggtt taactcagtt cgaaatgtgg agtgagttta ctgctgatgg attcctacgt
     4141 tatccaaact tcttggaaac agtaactcaa ttgattccaa tgtatgcgct tcgctcttta
     4201 ggtggtacgt tatacttagt tggtttcttg gtaatgattt acaacttatg gaaaacagca
     4261 tttgctggta aattcattgc aaacgaagct gccgaagctg ctccacttcc aaaaggcgca
     4321 actccaggtc accaagcttc agaaggatgg caccgtgtaa ttgaacgtaa gccatttcaa
     4381 atgctattgt tcagcttaat catgattctg attggtggtt tagttgaaat gattccaaca
     4441 ttcttgatta agagtaatat tccaacaatt gcttctgttc aaccttacac tccacttgaa
     4501 cttcaaggtc gcgacattta tatccgcgaa ggttgctata cctgtcacag ccaaatgatt
     4561 cgtccattcc gtttcgaaac agaacgttat ggtgaatatt caaaagctgg agaatatgtt
     4621 tatgaccatc cattccagtg gggatcaaaa cgtacaggtc cagatttgca aagagaagga
     4681 ggaaaatatc ctgatagctg gcactacaac cacatggatg atcctcgcac catgtcacca
     4741 ggttctatca tgccaatgta tccttggtta cttgacgatg atttagatac tacatcaact
     4801 gctcctaaaa ttcgtgctat gatgactttg ggtgtacctt atcctgaagg ctatgataag
     4861 attgctaacc aggacttggt tcgtcaacaa aacaaaattg taaataacct taaagagtct
     4921 ggtatcgaag ccttgcctaa caaagaaatc atcgccatga tttcctactt gcaacgtctt
     4981 ggaacagaca tcaaaggaga gaacaaaatt gtctttggtc aaattgctac aaccaagcc
//
LOCUS       DAOOQW010000175         5037 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_19981_length_5037_cov_4.718386, whole genome shotgun sequence.
ACCESSION   DAOOQW010000175 DAOOQW010000000
VERSION     DAOOQW010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5037
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19981_length_5037_cov_4.718386"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..829
                     /locus_tag="PK281_09180"
     CDS             <1..829
                     /locus_tag="PK281_09180"
                     /inference="COORDINATES: protein motif:HMM:NF014708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HOY49560.1"
                     /translation="YPMGKLRVRQPFPSHQVDYPDPFLLLHHANMEIPKTESLADAGV
                     GPHPHRGFSPVTFIFKGGVHHRDSRGNNSIIYEGGVQWMNSGMGIIHSERPPFDIHER
                     GSRQEIIQMWVNTPAANKMDQPEYHPVSAEETPQFETPDQLITAYVVTGKLFGLKGPV
                     PTATEVNSATIEATKGGRISIEIPDTHEAFLYLLDGKISLENYGLAEMHQAVLFNKDG
                     NTISFEALENTRMLFMSGKPLNEPMASHGPFVMTNETELLQAMRDYQMGKMGVLIEE"
     gene            975..2390
                     /locus_tag="PK281_09185"
     CDS             975..2390
                     /locus_tag="PK281_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49561.1"
                     /translation="MKLKLIVLLLMGIISSNKIEAQKIDSTLYTALSEVYSLMQVKAD
                     KSDSLFVNTVSKFIQQIELTSPTQPIIKTDSLNNSKPKVDNENKQVDSLKNVAFQFSF
                     VPPVSSNGMDNSKCRNQFSVNMLAGYAGAVNGLEIAGFANVLRKEMHGLQLAGFANIT
                     AGETEGIQLAGFCNVNQKKVDGIQVSGFSNVVSDSVQGVQIGGFCNVNSGSVSGFQQA
                     GFANINGGKTDGFQCAGFVNSVSDSTTGFQIAGFVNQSSQKMDGVQLAGFMNVAQQEL
                     AGVQVSGFINTCRRLKGTQIGFINYADTLESGTPFGFLSVIRKGGFQALEVGASETFP
                     ATFQWKTGSDVFYNIFNIGAMPGNNFYWGYGYGFGSNLKKTGKFRINLDLLAMHVNNG
                     EIWTDHVNLLNKAHLGFTWQAAKHFAISGGPTFNVLVVNNPDSELTVQQPTVAPWTVS
                     DKMYNNTRVQMWPGAQLMIRF"
     gene            2416..4749
                     /locus_tag="PK281_09190"
     CDS             2416..4749
                     /locus_tag="PK281_09190"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOY49562.1"
                     /translation="MLRYFLLACFAVQSCLAISQNLVQTIRGTVVDQTTENPIPGASV
                     VILNSNPLIGTTCDLDGHFRLPQVSIGRYDLKISLIGYQDLILPNVLVGAGKEIVLNI
                     KISERVTEIKAVEIVAGSNNKAEAINELASVSARQFSVEETKRYAGALNDPGRMALSF
                     AGVTSNSDGSNEIVVRGNSPRGLLWRMEGIEIPNPNHFSNQGSAGGAISMLSVNMMAN
                     SDFFTGAFPAEYGNAASGVFDIRLRKGNNEKRETALQIGVIGTEAALEGPIKKGYSGS
                     YLINYRYSSLALLQKLGVKIVGDAIPKFQDLSFNINLPTKNAGNFTLFGIGGISQIYE
                     EYLGKQVQYRSNFKTNLGVAGITHSYMFNEKTWLKSVVAMTSTSNDYLNSRYDTLAVF
                     QHDDIDEKFIHSALRASITLNHKFNRKHSLKSGFIASKLGYSMKSVMYDYELEKPIVN
                     LDKNGNTGLFQAFANWQYRISDKWTLNSGVHYSRFLLNGSEAFEPRIGVKWNVKGSQI
                     LSAGIGLHSRLEDLPIYYGQVQLSDGSIINPNKNLGYSKARHYVFGYETMLTEHLHVK
                     METYYQDLYAIPVDKTELNGLSMINMSQGFTTIAAKNSGTGYNYGYELTLEKYFSKRY
                     YFMFTGSIYDSKYRGSDNVLRNTSYNGNYASSFLAGKEFKMGNRNTLSLNIRAVFAGG
                     RRYTPVLLEESIAQGYGIYDNSRVNELQASAYWRTDLGISYTINKNKTTRVWKIDIQN
                     ATNRLNEYGRYFNDDSRKVEIATQAGIIPTLSYRLEF"
     gene            4849..>5037
                     /locus_tag="PK281_09195"
     CDS             4849..>5037
                     /locus_tag="PK281_09195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49563.1"
                     /translation="MLKLLRLILPTLLILTGFLSNGQVVINEFSCSNISTIGDDIGNF
                     EDWIELHNPTGAAVNIGGC"
BASE COUNT         1676 a          852 c         1029 g         1480 t
ORIGIN      
        1 atacccgatg gggaaattgc gtgttcgtca gccttttcca tcacatcaag tagattatcc
       61 agatccattc ctattgctgc atcatgccaa tatggaaata cctaaaactg aaagtctagc
      121 ggatgccgga gttggtccac atccacatcg tggtttttct cctgtgacgt tcatctttaa
      181 aggtggtgtt catcatcgag atagtcgagg aaacaacagt atcatatacg aaggtggtgt
      241 tcaatggatg aattcaggaa tgggaatcat ccacagtgag cgtcctccat ttgatattca
      301 tgaaagagga agtcggcagg aaataattca aatgtgggta aatactcccg cggctaataa
      361 aatggatcaa cccgaatatc atcctgtttc agctgaggaa acaccgcaat tcgaaacacc
      421 agatcaactt ataactgcct atgtggtaac tgggaaatta tttggattaa aaggtcccgt
      481 tccaactgct accgaagtga attctgctac tatcgaagca acaaaaggag gaagaattag
      541 cattgaaatt cccgatacac atgaagcatt tttgtatttg ttggatggta aaataagctt
      601 ggagaattat ggtctagcag aaatgcacca agctgtatta ttcaataaag atggcaatac
      661 catttctttt gaagcattag aaaatacacg gatgcttttc atgagcggaa aaccattgaa
      721 tgaaccgatg gctagtcacg gtccttttgt gatgacaaac gaaactgaat tgcttcaggc
      781 gatgcgtgat taccaaatgg gaaaaatggg tgtacttatc gaagaataaa gaattcataa
      841 atcatgaaaa gagctgccaa aaacagctct tttttatttt taataaaatc gaaaataaat
      901 aagcttaaaa atatttgtcc ttcgaatagg ggttagttaa agttgaattg tcttgctttt
      961 gaaaactcaa caagatgaaa ctaaaactaa tcgtattgct tttgatggga attatctcaa
     1021 gcaataaaat cgaagctcaa aagattgatt caaccttata tacagcctta tctgaggtat
     1081 attctttaat gcaagtcaag gcggataaaa gtgattcgct ttttgtgaat acggtttcaa
     1141 aattcattca acaaatcgaa ctcacatccc ctacacaacc aataataaaa accgattcgt
     1201 taaataactc gaagcctaaa gttgataacg aaaataaaca ggttgattcc ttaaaaaacg
     1261 ttgcctttca attctcattt gttccaccag tgagtagcaa cggaatggat aattccaaat
     1321 gccggaatca gttttcagtc aatatgttgg ctggttatgc gggtgctgta aacggacttg
     1381 aaatagctgg ttttgctaat gttcttcgaa aagaaatgca tggattacaa ttagctggat
     1441 ttgccaacat tacagctggt gaaacagaag gaatccaatt agctggtttc tgtaatgtaa
     1501 atcagaaaaa ggtagatgga attcaagttt caggatttag caacgtcgta agtgattcgg
     1561 ttcaaggtgt tcaaattgga ggattttgta atgtgaatag cggttccgtt tcgggtttcc
     1621 aacaagcagg atttgccaat attaatgggg gaaaaacaga tggattccaa tgtgctggat
     1681 ttgtaaattc tgtatccgat agcaccactg gattccaaat agctggattt gtaaatcaat
     1741 cctcccaaaa aatggatggt gttcaactag ctggcttcat gaatgtggcg cagcaagaat
     1801 tagccggcgt tcaggtttcg ggatttatca atacatgtcg tcgtttaaaa ggaacacaaa
     1861 taggatttat caattatgcc gatacgcttg aaagtggaac tccattcggt tttttaagtg
     1921 tcattcgaaa aggaggattt caagcattgg aagtaggagc atcagaaaca tttccagcca
     1981 ctttccaatg gaaaactgga agtgatgttt tctataacat cttcaatatt ggcgcaatgc
     2041 caggaaataa cttttactgg ggatatggct atggctttgg tagcaatttg aaaaagactg
     2101 gaaaattccg gatcaatctc gatttgttag ctatgcatgt gaataatgga gaaatctgga
     2161 ccgatcatgt taatctactc aacaaagcac atctcggatt tacttggcaa gctgctaaac
     2221 actttgctat tagcggtggt cctactttca atgttctggt agtaaataat cccgattcag
     2281 aattaacggt gcaacaacca accgtagcac cttggactgt atccgataaa atgtacaaca
     2341 atacgcgagt acagatgtgg ccaggagccc aactaatgat tcgtttctaa ttccaattaa
     2401 cccaacccaa tttttatgct tagatatttt cttctggctt gctttgccgt gcaatcttgc
     2461 ctagcaattt cgcagaattt agtacaaacc atccgaggaa cagtagtaga tcaaacgacc
     2521 gaaaatccaa taccgggagc gagtgttgtc atccttaatt ccaatccact aattggaaca
     2581 acctgcgatt tagatgggca tttccgtttg cctcaagttt ccattggcag gtatgatttg
     2641 aaaatatccc tcattggata tcaggactta atattgccta atgtgctggt aggcgcagga
     2701 aaggaaattg ttttgaatat taaaatcagc gaacgtgtta ccgaaattaa agcagttgaa
     2761 atagtagctg gaagtaataa taaagcagag gcaatcaatg aattagcatc tgtaagtgcg
     2821 agacaatttt cagtagaaga aacgaagcga tatgcaggcg cattgaacga tccgggacga
     2881 atggcacttt catttgcagg tgttactagt aattcggatg gttcaaatga aattgtagta
     2941 cgaggaaatt cgcctcgcgg tttgttatgg agaatggaag gaattgagat tccaaatccc
     3001 aatcactttt caaatcaagg ctcagcaggt ggtgcaatca gtatgctaag tgtgaatatg
     3061 atggctaatt ctgatttttt cacaggtgct tttccagcag aatatggaaa tgcagcatca
     3121 ggtgtattcg acatccgact tcgtaaagga aataacgaaa agcgtgaaac tgcgctgcaa
     3181 attggtgtta tcgggacaga agctgcttta gaaggtccaa taaagaaagg ttattcagga
     3241 tcatatctga ttaattatcg gtattcatct ttggcattat tgcaaaagtt aggagttaag
     3301 atagtaggtg atgcaattcc taaattccaa gacctctcat tcaacattaa tttaccaact
     3361 aaaaatgctg ggaatttcac tctttttgga attggtggaa ttagccagat ttatgaggaa
     3421 tatctaggaa aacaagtgca ataccgaagt aatttcaaaa cgaatcttgg tgttgcaggg
     3481 attacacata gctatatgtt taacgaaaaa acttggctaa aatcggttgt tgctatgacc
     3541 agtacaagca acgattacct caattcacgt tatgacacac tggctgtttt tcaacacgat
     3601 gatatcgatg aaaaatttat ccattctgcc ctacgcgctt caattacatt aaatcataaa
     3661 ttcaacagaa aacacagcct gaaatcaggc tttatagcaa gtaaattagg ctattcaatg
     3721 aaatcagtta tgtatgatta tgaattagaa aaacctatcg taaatctcga taaaaatgga
     3781 aatacagggt tatttcaagc atttgcaaac tggcaatatc gcataagtga taaatggaca
     3841 ttaaactctg gagttcatta ttcacgcttt ttattgaatg gttctgaagc gtttgaacca
     3901 agaattggag tgaaatggaa tgtgaaaggt tcgcaaatat tgagtgctgg aattggttta
     3961 cacagtcgct tagaagattt gcccatttac tacggacaag tgcaattgag cgatggaagc
     4021 ataataaatc cgaataaaaa tttaggttac tctaaagcaa gacattatgt attcggttat
     4081 gaaacaatgc taaccgaaca tttgcatgtt aaaatggaaa cttattatca ggatttatac
     4141 gccattccgg ttgataaaac agaattgaat ggtttatcga tgataaatat gtcgcaagga
     4201 ttcacaacaa ttgctgctaa aaattctgga acaggttata attatgggta tgaattgaca
     4261 ttagaaaaat acttctcaaa acgttattac ttcatgttca cagggtcgat ttatgattcc
     4321 aaataccgtg gttctgataa tgtcttaaga aacacttctt acaatggaaa ttatgcaagt
     4381 agtttcttgg cagggaaaga atttaaaatg ggaaatcgaa atacgttaag cttaaatatt
     4441 cgagctgttt tcgctggagg aagaagatat acgcctgttt tattggaaga gtcaatagca
     4501 caaggttatg gtatttatga taattcgcga gttaatgaac ttcaagcttc ggcctattgg
     4561 agaaccgatc ttggtattag ctacacgata aataaaaata agaccactcg agtttggaaa
     4621 atcgacattc aaaatgcaac aaaccgattg aatgagtatg gtagatattt caatgatgat
     4681 tcgagaaagg ttgaaattgc tacacaagca ggaataattc caacactaag ttaccgacta
     4741 gagttttaat gtatcagatt tgaatacgta agagttttaa taatttattt gagcaaaatg
     4801 gaacattttc ataaatttga agattgtacc tgaccacagc ttatcaaaat gctaaaactc
     4861 ttacgtttaa ttctacctac actccttatt ctgacaggat tcttgtcgaa tgggcaggtt
     4921 gtaatcaatg agttttcatg ttccaatatt tcaaccatcg gcgatgatat tggaaacttt
     4981 gaagattgga ttgaacttca caatccaaca ggtgcggcag taaatattgg aggttgt
//
LOCUS       DAOOQW010000176         5027 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_20038_length_5027_cov_3.972848, whole genome shotgun sequence.
ACCESSION   DAOOQW010000176 DAOOQW010000000
VERSION     DAOOQW010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5027
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20038_length_5027_cov_3.972848"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1057)
                     /gene="hemN"
                     /locus_tag="PK281_09200"
     CDS             complement(<1..1057)
                     /gene="hemN"
                     /locus_tag="PK281_09200"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HOY49564.1"
                     /translation="MQTDLLRKYAVPGPRYTSYPTVPYWDSGRPEEESWENRSLQTYL
                     RNNGENGISLYIHLPYCESLCTYCGCTTRITRNHLVEKPYILAVLNEWSKYLDLFATT
                     PRLAELHLGGGTPTFFSPENLKFLLDGILGTVVVTPDAEFSFEGHPGNTTREHLETLF
                     ELGFRRVSFGIQDFDVKVQDAIHRFQTVEEVETITRTAREIGYTSINYDLIYGLPFQT
                     LSGITTTIEEVVRLKPDRIAFYSYAHVPWIKPGQRKFTEMDLPLPDQKRELYEKGREM
                     LENAGFTEIGMDHFALKNDSLMLAEAEGRLNRNFMGYTTTQSELLIGLGASSISDCGT
                     AYVQNEKKVEDYLARVKK"
     gene            complement(1064..1789)
                     /locus_tag="PK281_09205"
     CDS             complement(1064..1789)
                     /locus_tag="PK281_09205"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOY49565.1"
                     /translation="MELLIAAFSLGILGSFHCVGMCGPIALALPVHQLSPQGRIAGII
                     LYNVGRAFTYAVLGAIFGALGMGVILAGYQQTLSIVLGVLLLLTVVLPSVKKDGFFSV
                     VFPQLAFWLKARFSYLFSKRSYTALFLTGVFNGLLPCGLVYMALAGATASGDIFLGAL
                     FMAVFGLGTLPAMFSVTLFSSLSASFRSSMRKLAPIVAGVMACLLILRGLGLGIPYIS
                     PELSHKDNTEHSCCHKPGMQQEH"
     gene            complement(1796..2251)
                     /locus_tag="PK281_09210"
     CDS             complement(1796..2251)
                     /locus_tag="PK281_09210"
                     /inference="COORDINATES: protein motif:HMM:NF017559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HOY49566.1"
                     /translation="MKNSKIWTWGTGIAIVYSLFALGIIGMVVNSFGKKLDLTSKDYY
                     AKELVFQEQIDKSNRSKALAEPLSWKVETGKLLISYPASFTGKKIGGTITLFKPSNNN
                     SDVNFPVQANAEMKQEIPTGTLGKGMYKLRIDWSADAQTYYNEGVVVLN"
     gene            complement(2274..3695)
                     /gene="ccoG"
                     /locus_tag="PK281_09215"
     CDS             complement(2274..3695)
                     /gene="ccoG"
                     /locus_tag="PK281_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HOY49567.1"
                     /translation="MDSDPKRENPTHDESFRDSINTITADGKRNWIYPQRPKGKFYNR
                     RTIVSIVYLILFFTIPFIKLDGDPLVLMNIVERKFIIFGLVFFPQDLFLFALAMLTFM
                     VFIVLFTVIFGRLFCGWACPQTIFMEMVFRKIEYWIEGDANKQRQLDKGPWNLEKIRK
                     KGSKTFIFFVLSFLFGNFFLSYIIGMDELISIVQEPLSKHYGGFSAMIFFTGAFFFVY
                     MYFREQVCLVVCPYGRLQGVLLDRNSIMVAYDYVRGEPRGKHHKGDDTSKLGDCIDCG
                     LCVRVCPTGIDIRNGTQLECVNCTACIDACDGIMEGIHKPKGLIRYDSENGIASGKKL
                     TFTLRMIGYSIILVGLMVGLSFGIAGRSKVEATILRAPGMMFQEVGKDSISNLYNFKI
                     VNKTKEEMKLDLILESPAGVIKHVGSKEKFNIPSAGLAEGTFFVTIARKDLEGRKNEI
                     KFKLNVDGETLENTKTNFLGPVK"
     gene            complement(3711..4928)
                     /locus_tag="PK281_09220"
     CDS             complement(3711..4928)
                     /locus_tag="PK281_09220"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HOY49568.1"
                     /translation="MKTVQSNARLNSSWMLLPAMLFTTLSAVAQQAAPEAAAATPVES
                     PSLMMNPTFIILLVTTLVLLGVILSLSGAIRNLSESGRAKLRKDKISSLSPILALAFL
                     LPLSSSAQEVATGPTTDKWMPPVQIAGLDSWIFVLMLVMIIIEFIIILGLLKSIKQLL
                     IGFGFQPAPDMENVKPWINWKWLDRQLTDAVPLEQEATVMTDHEYDGIRELDNNLPPW
                     WKYGFYFTIVFGIVYLFNYHVSHSSPLQTQEYQSSMIEAELLKKEHLKEVGSNVDETN
                     VTILTDAASMASAKEIFTGNCAACHGQNGEGGVGPNLTDPNWIHGGGIQNVFKTIKYG
                     VPAKGMIAWQAQLKPEAMQKVASYILTLQGTNPANAKAPQGEVWVEPTTTPGDTSTVK
                     TDTTKAMAATEVK"
BASE COUNT         1452 a         1051 c         1039 g         1485 t
ORIGIN      
        1 ccttttttac acgagcaagg tagtcttcta ctttcttctc attttgcaca taagctgttc
       61 cgcaatcact aatggaagat gctcctaaac caatcaggag ttcactttgg gttgtggtat
      121 aacccatgaa attccgattg agtcgtcctt ctgcttcggc aagcatgagg gaatcgtttt
      181 tcaaagcaaa atgatccatg ccgatttctg taaatccagc attttcgagc atttcacgac
      241 ctttttcata tagctccctt ttttgatcgg ggagaggaag atccatttcg gtgaatttgc
      301 gttgaccagg ttttatccaa ggaacatggg cataactata aaaggcaatc cgatcaggct
      361 ttaaacgaac cacttcttcg atggtagtcg taataccact taatgtttga aatggtaatc
      421 cgtaaattaa gtcgtaattg atcgatgtat agccaatttc gcgagccgta cgtgtaattg
      481 tttcgacttc ttctaccgtt tgaaatcgat ggatagcatc ttgcactttt acatcaaaat
      541 cctgaatccc aaaactaacc cgacgaaatc ctaactcaaa taaagtttct agatgttcgc
      601 gtgtggtgtt gcctggatgt ccttcaaagc tgaattccgc atcaggtgta acgaccacag
      661 tacctaaaat cccatccagt agaaatttca aattttcggg actgaagaat gtaggagttc
      721 ctccacctaa gtgcaattct gccaaccgag gtgtggtggc aaaaagatcg aggtatttgc
      781 tccattcgtt gagaacggca agtatgtaag gcttttcaac taagtgattg cgagtgatac
      841 gagtcgtaca tccgcagtaa gtacaaagac tttcacaata aggtagatgg atgtacaaac
      901 tgattccgtt ttcaccattg ttccttaagt aggtttggag agaacgattt tcccatgatt
      961 cctcctccgg ccgtcccgag tcccaatagg gaaccgttgg atagctcgtg tatcgaggtc
     1021 ccgggacagc atatttacgg agaaggtcag tttgcatggg aaattaatgc tcttgttgca
     1081 ttcccggttt gtgacagcag gaatgttctg tattgtcttt atgagaaagt tccggactga
     1141 tatacgggat accaagtcca agtcctctta aaatgagcag acatgccata acaccagcaa
     1201 cgataggtgc taatttacgc atagagctgc ggaatgatgc agatagagaa gaaaagaggg
     1261 tgacactgaa catagcgggc aaagtaccca acccgaacac cgccatgaac agtgcaccca
     1321 agaaaatatc gcctgaagca gtagctccgg caagtgccat atatactagt ccgcatggca
     1381 ataagccatt gaacacacca gttagaaaaa gagctgtgta cgaacgtttg ctgaacaaat
     1441 agctgaaacg cgctttaagc caaaatgcta actgaggaaa cacaacagag aaaaatccat
     1501 cttttttaac tgaaggaagt acaactgtta gaagtaagag aacacctaaa acaatcgaaa
     1561 gtgtttgttg gtaacccgct agaattacac ccattccaag tgcgccgaaa atggcaccta
     1621 gaacggcata ggtaaatgca cgaccaacgt tgtaaaggat gataccagca attcttcctt
     1681 gtggactaag ttgatgaacc ggtaatgcta atgctatagg tccgcacatt cccacacagt
     1741 ggaaacttcc aagtattcct aagctaaatg cagcaatcag gagttccatg acaacctagt
     1801 ttaaaacaac aacaccttcg ttgtaatacg tttgagcatc agccgaccaa tcgatacgga
     1861 gcttgtacat gccttttcca agcgttccgg ttggaatttc ctgtttcatt tcagcatttg
     1921 cttgaacggg gaagttcaca tccgaattgt tgttagaagg cttgaaaagc gtaatggttc
     1981 cgccaatttt ctttccagta aaagatgctg gatagctaat cagtaatttt ccggtttcca
     2041 ctttccagct gagcggctct gctaaagctt tcgaacggtt cgatttatcg atttgctctt
     2101 gaaatacaag ctctttagca tagtaatctt tcgaagtaag gtcgagcttc ttgccgaaac
     2161 tatttacgac cattccgata attccgaggg caaacaaaga ataaacgatt gctattccag
     2221 tgccccatgt ccagattttt gaatttttca tgatggttgg gttttacatg aaattactta
     2281 acaggtccga ggaagttcgt tttggtattt tccagcgttt ctccgtcgac attgagtttg
     2341 aattttatct cattctttct gccttccaga tctttacgag caatagttac gaagaatgtt
     2401 ccttctgcta atcctgctga tggaatattg aacttttctt ttgatccaac atgtttaata
     2461 acacctgccg gagattcaag aattaaatcc agtttcatct cttccttagt cttattgact
     2521 attttgaaat tgtacagatt actgatactg tctttaccaa cttcttggaa catcattcct
     2581 ggagcacgaa gtatagtagc ttctactttg cttctacctg cgattccaaa acttaagccg
     2641 accattagtc ctactaaaat gattgaatat ccaatcattc ttaaagtaaa tgttagcttt
     2701 ttgcctgatg caataccgtt ttcagagtcg taacgtatta atcctttagg cttatggatt
     2761 ccttccataa taccatcgca ggcgtcaata catgcagtac aattcacgca ttcgagctga
     2821 gtaccgttac gaatgtcgat tccagttgga caaacacgaa cacataaacc acaatcgata
     2881 caatcgccta gtttggatgt gtcatctcct ttatgatgtt ttcctctagg ttcgccacga
     2941 acatagtcgt atgctaccat gatagagttc cgatctaaga gtacaccttg taatcgtccg
     3001 taaggacaaa caactaagca tacttgctcg cggaaataca tgtaaacgaa aaagaaagct
     3061 ccagtgaaga agatcatcgc agagaatccc ccgtaatgtt tgctgagcgg ctcttgtacg
     3121 atgctaatca attcatccat cccgatgata taggaaagga agaagtttcc aaacaagaaa
     3181 gaaagaacaa agaagatgaa cgttttgctg cctttttttc ggattttctc caagttccaa
     3241 ggacctttat cgagttgacg ttgcttgttt gcatcgccct caatccaata ttcaatcttc
     3301 ctgaaaacca tttccatgaa aatggtctga ggacaagccc atccgcagaa caaccttcca
     3361 aagatgacgg taaatagcac gatgaacacc ataaatgtga gcatcgccag tgcaaacagg
     3421 aacaagtctt gaggaaaaaa gacaagtccg aaaatgatga actttcgttc aacgatattc
     3481 attaaaacca gtggatcacc atcaagtttt atgaatggaa tggtaaagaa cagaatcagg
     3541 tagacaatac ttacaattgt ccgtctgttg tagaacttcc ctttgggtct ttgcgggtaa
     3601 atccaattcc gttttccatc agcagtgatg gtgttaatgg agtcgcggaa ggattcatcg
     3661 tgggttgggt tttccctttt agggtcggaa tccatggtgt gggttgggtt ttatttgact
     3721 tcagtggcag ccatcgcttt ggtggtgtcg gtttttactg ttgatgtatc gcctggagtg
     3781 gtagttggct caacccaaac ttctccttga ggagcttttg cgtttgccgg attggttcct
     3841 tgtaaagtga ggatatagct ggctactttt tgcattgctt caggtttcaa ctgagcttgc
     3901 caagcaatca tcccttttgc aggaacacca tatttgatgg ttttgaatac attttgaatt
     3961 cctcctccat gtatccagtt aggatctgtc aagttgggac caactccacc ttcaccattt
     4021 tgaccatggc aggcagcaca gttgccagta aagatttctt tagcagaagc catagaagca
     4081 gcatctgtta agatggtaac attagtctcg tcaacatttg atcctacttc tttcaaatgc
     4141 tcttttttca acaactcagc ttcaatcatt gaagattgat attcctgtgt ttggagtgga
     4201 gagctgtgtg aaacgtgata gttgaagaga tacactatac cgaatacaat cgtaaaataa
     4261 aatccgtatt tccaccaagg aggaagattg ttgtccaact cacgaatacc atcgtactca
     4321 tggtcggtca taacagtagc ttcttgttcc aatggaacag catctgttaa ttgacgatcg
     4381 agccatttcc agttaatcca aggtttaaca ttttccatat ctggagctgg ttggaagcca
     4441 aatccgataa gaagttgttt gattgactta agtaatccta gaataatgat gaattctata
     4501 ataatcatta ctagcattaa tacgaaaatc caactgtcga gtcctgcgat ttgaacagga
     4561 ggcatccatt tatcagtagt tggtcctgtt gcaacttctt gagctgaact gctaagtggc
     4621 aataaaaacg caagtgctaa aatcgggctt agactagaaa ttttgtcttt gcgaagttta
     4681 gctcttcctg attcagaaag gttgcgtatg gctcctgaca agctcaaaat cactcctaat
     4741 aaaactaatg ttgttaccag aaggatgatg aatgttggat tcatcatgag tgatggagat
     4801 tctactggag ttgctgcagc tgcttcagga gcagcctgtt gggcaactgc tgacaatgtt
     4861 gtaaataaca ttgccggcag gagcatccaa cttgaattca aacgggcgtt agattgaact
     4921 gttttcatag ctgctgtttt ttactgttcg tcttgctcaa atgggagatt ttccatgatt
     4981 gctacttctt gtttgctggt ggtaaataaa tgccataaca gcgcaat
//
LOCUS       DAOOQW010000177         5015 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_20093_length_5015_cov_5.672581, whole genome shotgun sequence.
ACCESSION   DAOOQW010000177 DAOOQW010000000
VERSION     DAOOQW010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5015
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20093_length_5015_cov_5.672581"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1507
                     /gene="gltX"
                     /locus_tag="PK281_09225"
     CDS             <1..1507
                     /gene="gltX"
                     /locus_tag="PK281_09225"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018278581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOY49569.1"
                     /translation="SPTGTLHIGGVRTALYNYLFAHKLGGDFILRIEDTDQNRFVPGA
                     EAYIMEALNWLGINPSESPLHGGPHAPYRQSERKAMYREYAEKLLASGHAYYAFDTPE
                     ELEDMRAKLTAAKVASPQYNAVTRMSMKNSVTLSEDEVKRRLDAGEPYVIRMKLPRQE
                     EVRFHDIIRGWIVVQTQNMDDKVLFKSDGMPTYHLANVVDDYLMQITHVIRGEEWLPS
                     APLHVLLYRYLGWEEVMPQFAHLPLLLKPDGNGKLSKRDGDRLGFPVAPLNWVDPHTG
                     DTTSGYREKGYYPEAVLNFLCMLGWNPGTTQEMFTLDELVQLFSLERVSKAGAKFDFE
                     KAKWFNHQYLLKHSDAELAAEFMPLLQAAGFNRPIEFVTTVVGLVKERANFVNDFLEH
                     ARFFFEAPTTWDEATLSKKWKPETQGILEGWRDALATTNDYSAANLEVTSKAYLESIG
                     VGIGQVLQGFRILITGLPAGPSMFEVCSLLGKEEVAARFNKALEYTKTKFA"
     gene            1504..1860
                     /gene="folB"
                     /locus_tag="PK281_09230"
     CDS             1504..1860
                     /gene="folB"
                     /locus_tag="PK281_09230"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HOY49570.1"
                     /translation="MTKGKILLEGMEFFAYHGLYTKEREEGQWFRIDLEVETDLSKAA
                     NTDALTEAIDYVALYALIQKEMKIPSKLLEHVAKRIIDSLRMNFPALGKTKIKVTKLN
                     PPIEGKVAGVSIVMES"
     gene            2162..2235
                     /locus_tag="PK281_09235"
     tRNA            2162..2235
                     /locus_tag="PK281_09235"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2194..2196,aa:Cys,seq:gca)
     gene            2953..4392
                     /locus_tag="PK281_09240"
     CDS             2953..4392
                     /locus_tag="PK281_09240"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY49571.1"
                     /translation="MKTNYTLKMAVAALALSAATQTLEAQVYISNVISSPQGTMADAV
                     SPVMATRSDLTKTYGAPENNDIQGPENFYALGFGGEITVEMSGPICNGAGDDLTVFET
                     TWGYDCSTYPEKARVWASQDLCNWVELTSAANPICHNGSLDLGCLPWAKYLKIKDVSD
                     ITLFTIDADGYDVDGVSGNSTGCSFPVQTGLSRYAASGFLGNPQSTQGLTENNAPIPV
                     ARDNQNKMLGLPTNPFNVYANDVTTTPANNNFYSLGNGGSVILSFPYSLYNGPGADVQ
                     IFETSFNDVASRTCGNYPEKARVQGSCNGVDFYDLVILPGDAGNGEVAGTNVICRDGK
                     LDFGSLAVVNYIKITDITFVGVSNFPGDGDGFDVDAVLGLQNCQTSVAKITAEESSSD
                     IMEDAMMVEAYPNPASDFMSVSVRMNMADNVTFRVMDLTGRVLSNETINTNGDNFNYV
                     LNLNNYKTGIYLLSVEGNGARQVLKIVKQ"
     gene            complement(4446..>5015)
                     /locus_tag="PK281_09245"
     CDS             complement(4446..>5015)
                     /locus_tag="PK281_09245"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOY49572.1"
                     /translation="NGCSASYTEQNVVQVLEQPVAGFRTSPEVVFIGGDELELTSLSE
                     NALYCYYVIGNDTILGCTNTYNFTEEGVYSITQVVLNALGCTDEITHTVTVEYGTEYY
                     VPTAFSPNNDGNNDEFLVVGSEIKEFSIIIFDRWGNEIFTSDNIEKGWKGLTPGNQPM
                     PEGVYSYRLEMRSKLNKDIVKTGAVTLLR"
BASE COUNT         1456 a          979 c         1096 g         1484 t
ORIGIN      
        1 tagtcctacc ggaaccctgc atattggagg tgttcgtacg gctctttata attacctctt
       61 tgcccacaaa ttagggggcg attttattct tcgtattgaa gataccgatc aaaaccgttt
      121 tgttcccggt gccgaagcct acattatgga agctttaaat tggctgggaa ttaacccaag
      181 tgagagtccg ctgcatggtg gtccgcatgc gccttaccgt caaagtgagc gtaaagcgat
      241 gtaccgtgag tatgctgaga aattgttggc gagcggtcat gcttattatg catttgatac
      301 gcccgaggaa ttagaagata tgcgtgctaa gttaacggct gctaaagtgg cttcgccgca
      361 gtacaatgca gttacgcgta tgtcgatgaa gaactcggtc acgctgtcgg aagatgaggt
      421 aaaacgtcgt ttagatgcgg gtgagcccta tgtgattcgg atgaaactgc cacgtcaaga
      481 agaagtgcgt tttcatgata ttattcgtgg atggattgtg gttcaaacac agaacatgga
      541 tgataaggtg ctgtttaagt cggacggcat gcctacctat cacttggcga atgtggtaga
      601 tgattatttg atgcagatta cgcatgtgat tcgtggagaa gaatggttgc cttctgcccc
      661 actccatgtg ttgttatatc gctatttagg ttgggaagag gtgatgccac agtttgcgca
      721 tttgcctttg cttttgaagc ccgatggaaa tggtaaattg agcaaacgtg atggagatcg
      781 attaggattt ccggttgctc cattaaattg ggttgatcca cacacgggcg ataccacaag
      841 cggatatcgt gagaaaggat attatcctga agctgtgctg aacttcttgt gtatgttggg
      901 ctggaatccg ggtacaactc aggagatgtt cactttggat gagcttgttc aattgtttag
      961 cttggagcgt gtgagtaagg caggagcgaa attcgatttt gaaaaagcga aatggttcaa
     1021 ccatcagtat ttgttgaagc attcggatgc cgaattagct gctgagttta tgcctttgtt
     1081 gcaggcggct ggatttaatc gtccaattga gtttgtaact acggtggtag gtttagtaaa
     1141 agagcgtgca aatttcgtga atgattttct agagcatgct cgtttctttt ttgaggcacc
     1201 aacaacttgg gatgaagcaa ctttaagcaa gaaatggaag cccgaaacac aagggatttt
     1261 ggaaggctgg cgcgatgctt tagctacaac aaacgattat tcggctgcta atttagaagt
     1321 aacatccaag gcatatttgg aatcgattgg agtaggaatc ggacaggtgc ttcaaggttt
     1381 tcgaattttg attaccggct tacctgcagg accatccatg tttgaagtgt gtagtttgtt
     1441 agggaaagaa gaagtggcag cccgttttaa caaagccttg gagtatacca aaactaagtt
     1501 tgcgtgacca aagggaagat tttactcgaa ggcatggagt tctttgccta ccacgggctt
     1561 tataccaaag agcgcgagga aggacaatgg tttcgtattg atctggaagt agagactgat
     1621 ttgagcaaag ctgccaatac agatgcgtta accgaagcga ttgactatgt ggctttgtat
     1681 gccttgattc aaaaggaaat gaagatccct tcgaagctat tggaacatgt agctaaacgg
     1741 attattgata gccttcggat gaattttcct gcattgggta aaacaaaaat caaggttacg
     1801 aagttaaatc ctccgataga aggtaaagtt gccggagtta gcatcgtgat ggagtcgtga
     1861 tatgtggaaa ttcctcaaac agaagattag caagaaaaag gtatgtggca aatgtggcca
     1921 cagctttacc tgtgtagtaa aagaaggctg ttggtgtgat aagttaacga tctcgaaaga
     1981 gaacttggca aagattcgcc ggatgtatat ggattgctta tgtccgcaat gcttggccga
     2041 atttgtcgat ccaattaact cagagagaag catttcctaa attttcacgt aatcagtaga
     2101 ttttcgcaat ttttttcttg gctacgtgac tgattttact acttttgtca cccctcaaaa
     2161 cggttccgtg gccgagcggt taggcagaag tctgcaaaac tttgtacagc ggttcaaatc
     2221 cgctcggaac ctccaaatgc gaaagacgcc ttttcggcgt cttttttcgt taatgactgt
     2281 tagcggattc acgaatacca ataaaaaata aacatatcat gagctaaatc cgctcggaac
     2341 ctccaataat taaagacgcc ctttcggcgt cttttttcgt ttatgccctt ctctactcga
     2401 attttgatta taactctagc aaccccggat ttcaatccgg ggagggacga aatgccccca
     2461 aagcaaattc tgaaaagacg ccctttcggc gtcttttcta gtttatgccc ttctctactc
     2521 gaattttgat tataactcta gcaaccccgg atttcaatcc ggggagggac gaaatgcccc
     2581 caaagcaaat tctgaaaaga cgccctttcg gcgtcttttc tagtttatga ctataagcag
     2641 attcacgaac acccataaaa acgcacttaa tcagctcttt atccactcag taccttttct
     2701 ttaaattgtg agcactaata cttaaggatc atttacccaa taaaaacgat agacaactaa
     2761 catgatgtaa gaaacatatt tataataagt ataaataatt agctatttat tagcgctaac
     2821 gcactcattt acacccaaaa cccattaaat cgtcttaatt gtgaattaaa ttgttattaa
     2881 acgaccattt taacaaatag ggcaatatgt aagaaatata catttgttgt catactttaa
     2941 aacctaacac ccatgaaaac taattacaca ttaaaaatgg cggttgctgc acttgctctt
     3001 tctgcagcta cccaaacgct cgaagcacag gtttacatca gtaatgtaat ctcttctccg
     3061 caaggaacta tggcagatgc tgtttctcca gttatggcca ctcgttctga tttgactaaa
     3121 acgtatggtg ctcctgagaa caacgatatt cagggaccag aaaacttcta cgcacttgga
     3181 tttggtggtg aaattacggt agaaatgtcg ggaccaattt gcaatggtgc aggcgatgat
     3241 ttaaccgtat ttgaaacaac atggggttat gattgctcta cctatccaga aaaagcacgc
     3301 gtttgggctt ctcaagattt atgtaattgg gttgaactta cttctgctgc aaatccaatt
     3361 tgccacaatg gttcattaga tttaggttgc ctgccttggg cgaaatactt gaaaattaaa
     3421 gacgtttccg acattacact ctttactatc gatgcagacg gatacgatgt agatggtgtt
     3481 agcggaaatt ctactggttg ttcattccca gtccaaacag gtttgtcacg ttatgcagct
     3541 agcggattct taggaaatcc acaatcaact caaggtttga ccgaaaacaa tgctcctatt
     3601 cctgttgctc gcgataacca aaacaaaatg ttgggattac caactaatcc attcaacgtt
     3661 tacgctaacg acgtaacaac tacaccagct aacaataact tctactcact tggaaatggt
     3721 ggttcagtga ttctttcatt cccttacagt ctttacaatg gtccgggagc tgatgtgcaa
     3781 attttcgaaa catcttttaa cgatgtagct agccgtactt gcggaaacta tcctgaaaaa
     3841 gctcgtgtac aaggatcttg caatggagtt gatttctacg atttagttat ccttccgggc
     3901 gatgctggaa atggtgaagt agcaggtacg aacgtaattt gtcgcgatgg taaattggat
     3961 tttggaagtc tagctgtggt gaattacatc aaaattactg acatcacttt cgtaggagtt
     4021 tctaacttcc ctggcgatgg cgatggcttt gacgtggatg cggtgttagg tttacaaaac
     4081 tgccaaacaa gtgttgctaa aatcactgcc gaagaatcaa gcagcgatat catggaagat
     4141 gctatgatgg ttgaagctta tccaaatcca gcaagcgatt tcatgagtgt ttctgttcgt
     4201 atgaacatgg ctgataatgt gactttccgt gtaatggatt taactggtcg tgtgttatct
     4261 aacgaaacca tcaatacgaa tggagataac ttcaattatg tattgaactt gaacaactac
     4321 aaaaccggaa tctacctact atctgtagaa ggtaatggag ctcgtcaggt attgaaaatt
     4381 gtaaaacaat aattgatttt aattcgaata aaaaaggctg ctcatatcgg gcagcctttt
     4441 tttatttaac gaagtagcgt gacagctccc gtttttacaa tgtctttatt gagcttactt
     4501 ctcatttcta gtcgatacga ataaactccc tctggcatag gttgattccc cggagtaagt
     4561 cctttccagc ctttttcaat attatcgctg gtaaaaatct catttcccca acgatcgaaa
     4621 atgataattg agaattcttt tatctcagag ccaactacta agaattcatc attatttcca
     4681 tcattattcg gagagaaggc tgttggaaca tagtattctg ttccatattc gacagtaact
     4741 gtatgagtga tttcatcagt acaacccaat gcattcaata ctacttgagt tatcgaataa
     4801 acaccttctt cggtgaagtt atatgtattg gtacagccta aaatggtgtc gttaccaatc
     4861 acataataac agtaaagagc attttccgat aagctggtta attccaactc atcaccacca
     4921 ataaacacaa cttcaggact tgttcggaaa ccagcaacag gctgttccag cacttgaact
     4981 acattttgtt ctgtgtatga agctgaacaa ccatt
//
LOCUS       DAOOQW010000178         4982 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_20260_length_4982_cov_3.651106, whole genome shotgun sequence.
ACCESSION   DAOOQW010000178 DAOOQW010000000
VERSION     DAOOQW010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4982
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20260_length_4982_cov_3.651106"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="PK281_09250"
     CDS             complement(<1..283)
                     /locus_tag="PK281_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOY49573.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITSVLADQGLAEI
                     RDFSSIDNAPEEKERGITINTSHVEYATANRHYAHVDCPGHADYVKNMVT"
     gene            complement(388..459)
                     /locus_tag="PK281_09255"
     tRNA            complement(388..459)
                     /locus_tag="PK281_09255"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(425..427),aa:Thr,seq:ggt)
     gene            complement(556..631)
                     /locus_tag="PK281_09260"
     tRNA            complement(556..631)
                     /locus_tag="PK281_09260"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(596..598),aa:Gly,seq:tcc)
     gene            complement(699..783)
                     /locus_tag="PK281_09265"
     tRNA            complement(699..783)
                     /locus_tag="PK281_09265"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(747..749),aa:Tyr,seq:gta)
     gene            complement(826..898)
                     /locus_tag="PK281_09270"
     tRNA            complement(826..898)
                     /locus_tag="PK281_09270"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(863..865),aa:Thr,seq:tgt)
     gene            complement(1055..1354)
                     /gene="raiA"
                     /locus_tag="PK281_09275"
     CDS             complement(1055..1354)
                     /gene="raiA"
                     /locus_tag="PK281_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HOY49574.1"
                     /translation="MKVNIQSVHFNADKKLLDFIELKLQKLATHYDKIIESEVTLRLD
                     NASNNENKIVEIRLHVPGNDLFAKRQCATFEEAVDESIDALRNQMIKHKEKIRQV"
     gene            1495..2580
                     /locus_tag="PK281_09280"
     CDS             1495..2580
                     /locus_tag="PK281_09280"
                     /inference="COORDINATES: protein motif:HMM:NF014653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HOY49575.1"
                     /translation="MNYSYNSLLSFTHNVFRQMGCSENDAQTAAKVLVSADLRGIDSH
                     GVARLSGYHRLWEKKRINTQPIIKIVHETPSTATVQADSALGLVAAPFAMQLAIDKAK
                     IAGSGWVAVKGSNHFGIAGEHAMMALNENMIGIAMTNASPLVAPTWSTDRLLGTNPMA
                     FAIPAGNEPAFVADFATTTAANGKLEILQRTGKQAPDGWIQTKDGSSSSDPFSLSSGG
                     ALLPLGSDKEHGSHKGYCLGSVVDIFSAVLSGANYGPWVPPFVAFLDPPNDPVGEGIG
                     HFFGAMRIDAFRPADEFLFHMDNWIRTFRSAATVAGQEQVVIPGDPEREMAIIREKEG
                     IPLNEKVISDLKSLADKLQITFPNPNN"
     gene            complement(2591..3094)
                     /locus_tag="PK281_09285"
     CDS             complement(2591..3094)
                     /locus_tag="PK281_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010180691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOY49576.1"
                     /translation="MRIGLLSDTHGYLDEGILKHLHSCDEIWHAGDIGSKEVTDRLAS
                     IKPLRAVYGNIDGNDLRKEFPEDNRFICEGVDVLITHIGGYPGRYSQHAAQLIRTAPP
                     KLFICGHSHILKILPDKKFGLLHMNPGAAGQHGFHLIRTMIRFSIDDSRIHSAEVIEL
                     GRRGAII"
     gene            complement(3091..3717)
                     /locus_tag="PK281_09290"
     CDS             complement(3091..3717)
                     /locus_tag="PK281_09290"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOY49577.1"
                     /translation="MEITLNEVGKRYNFNWIFNGLSWHIASGDRCVFLGANGSGKSTL
                     LQIVAGSILSSAGKIQWRINEQEIEPEHVYQYVSIAAPYLELIEEFTLTELIHFHFSV
                     KKSINNLSESEILQKSGLTHAADRRISYFSSGMKQRVRLILAILSDTPLLLLDEPLSN
                     LDSRASEWYRALLEEFGGSRTILVSSNHQEPEYFFCNRRLLIEDFHRS"
     assembly_gap    3797..3806
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3807..4533)
                     /gene="lpxA"
                     /locus_tag="PK281_09295"
     CDS             complement(<3807..4533)
                     /gene="lpxA"
                     /locus_tag="PK281_09295"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007649251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HOY49578.1"
                     /translation="MNQTLASIHPDAKIGEGVTISPFVTIYGDVTIGEGTWIGPNVTI
                     MDGARIGKHCRIFPGAVISAIPQDLKFNGEVTTVEIGDYTTIRECATINRGTTDRMKT
                     VVGHHTNIMAYAHVAHDCIIGNYCILGNAVNMAGHVVVDDYAILSGMSAIHQFVHIGA
                     HVMVSGGSLVRKDIPPFVKAAREPMSYVGINSIGLRRRGFSNERISEIQDIYRVLFQS
                     GLNTTKAIQKIETDMPASPERDEI"
     gene            complement(4570..4848)
                     /locus_tag="PK281_09300"
                     /pseudo
     CDS             complement(4570..4848)
                     /locus_tag="PK281_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007649252.1"
                     /note="catalyzes the zinc dependent deacetylation of
                     UDP-(3-O-acyl)-N-acetylglucosamine to
                     UDP-3-O-(3-hydroxytetradecanoyl)-glucosamine in the second
                     step of lipid A biosynthesis and catalyzes the dehydration
                     of beta-hydroxyacyl-ACP to trans-2-acyl-ACP in fatty acid
                     biosynthesis; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine
                     deacetylase"
     gene            4844..4982
                     /locus_tag="PK281_09305"
                     /pseudo
     CDS             4844..4982
                     /locus_tag="PK281_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1455 a         1045 c          920 g         1552 t
ORIGIN      
        1 cagtaaccat gttctttacg taatccgcgt gacctggaca gtcaacgtga gcgtaatgac
       61 ggtttgctgt tgcatactca acatgcgaag tattgattgt aatacctctt tctttctctt
      121 caggagcatt gtcgattgaa gaaaagtctc tgatttcagc taatccttga tctgctaata
      181 cagatgtgat agctgctgtc aaggtagttt taccatgatc aacgtgacca atagtaccga
      241 tgttaacgtg cggtttggag cggtcgaatt tttcttttgc cattgcaatt ataaatttaa
      301 gttgtgttta tttatttagt taatgagaaa tctcaattaa ttttttaatc ttatttaatt
      361 ttttttgaag ttgttcctag ccttttgagc cgttggaggg aattgaaccc tcgacctctt
      421 ccttaccaag gaagtgctct gccactgagc tacaacggcc ggtaagaatt tagccgcaac
      481 ttctaaaaaa atggagtgga agaccggtct caattgttta tattatcata aactttttgc
      541 acgctcttct ctccttggag cggaagaccg ggctcgaacc ggccaccctc agcttggaag
      601 gctgacgctc taccaaatga gctacttccg cttgaccata ctttcttgtt caagaccaaa
      661 atcctaaact ttcgaatatg cacctgttct cttttccttg gtggggagag aaggattcga
      721 accttcgaac tcggagagga cagatttaca gtctgttgtc gttgaccgct tgactatctc
      781 cccaacctct ttgaagaaat aaaaaaaaat tactacaaag aacagagccg aagaagggac
      841 tcgaaccccc gaccagctga ttacaaatca gctgctctac cagctgagct acttcggctt
      901 ttaccttttt tgatttatga tttttgctat cgcatctatc acccttcaaa aaaggtgtgc
      961 aaatgtaggt aagaaatcat tcccaccaaa catttttaaa aaattctgca gctattttta
     1021 cacagcgcaa atcaaaatct ttagaatcag aattttagac ctgtcgaatt ttttctttat
     1081 gcttaatcat ttggtttctc aatgcatcaa ttgattcatc taccgcctcc tcaaatgtag
     1141 cacattgtcg ttttgcaaac aaatcatttc caggcacatg caaacgaatc tcaacaattt
     1201 tattctcatt gttgctcgca ttgtccaaac gaagggtcac ttcactttct attatcttat
     1261 catagtgtgt tgctagtttt tgaagtttta gctcaataaa atcaagtaat ttcttgtctg
     1321 cattaaaatg cacggattga atgttcactt tcatagttga atttgtttag agttgaaatc
     1381 cggattcctt atttcaaaag ccggacgaca agaatcgcta aattatatat ttgtgcgctt
     1441 agtggcaatg tgaattttac ccattacact tgttaatatc ctgtttattc tctcatgaac
     1501 tattcctata attctctgct ttcattcact cacaacgttt tccgtcaaat gggctgctcc
     1561 gaaaacgatg ctcaaactgc tgcgaaagtt ttagtttcag ccgatttacg aggcattgat
     1621 tcgcatggtg ttgctcgttt aagtggttat caccgtcttt gggagaagaa acgaatcaat
     1681 acgcaaccca taattaaaat tgttcatgaa actccatcta cagctactgt tcaggcagat
     1741 agtgctcttg gattagttgc tgcaccattt gctatgcaac ttgcgattga taaggctaaa
     1801 atcgcaggtt cgggttgggt agcagtaaaa ggttcaaacc attttggtat tgctggtgaa
     1861 catgccatga tggctctaaa tgaaaatatg attggcatcg ccatgactaa tgcaagtcca
     1921 cttgttgctc caacttggtc aaccgatcgt ttattaggca ccaatccaat ggcatttgct
     1981 attccagcag gaaatgaacc tgcttttgtt gccgattttg ctactacaac agcagcaaat
     2041 ggtaaacttg aaatattaca gcgcacagga aaacaagctc ccgatggatg gatacaaacg
     2101 aaagatggaa gttcttcaag tgaccccttt tcactttcat ctggaggcgc tcttcttcca
     2161 ttaggtagtg ataaagaaca cggtagtcat aaaggctact gtttaggttc tgttgttgat
     2221 attttttcag cagtactttc tggtgccaat tatggtcctt gggtgccacc ttttgttgct
     2281 ttccttgatc cacctaatga tcctgttggc gaaggaattg gacatttctt tggagccatg
     2341 cgaattgatg cttttcgtcc agcagatgaa tttttatttc acatggataa ctggattaga
     2401 acatttcgtt ctgctgcaac tgtagctgga caagaacaag tagtaatacc aggtgatcca
     2461 gaaagagaaa tggccatcat tcgtgaaaaa gaaggaatcc ccttaaatga aaaggtgatt
     2521 agtgatttaa aatcgcttgc tgataaactt caaattacct ttcctaaccc aaataactag
     2581 cattagggaa ttaaattatt gctcctctcc tacccaattc aataacttca gctgagtgaa
     2641 ttcggctatc atctattgaa aaacgaatca tagttctaat taaatgaaac ccatgctgac
     2701 ctgcagctcc tggattcatg tgcaataacc caaatttttt atcgggaagt attttgagga
     2761 tatgcgaatg cccacaaatg aagagttttg gtggagcagt tcgaattaat tgcgctgcat
     2821 gctgggaata cctgcctgga tatccaccta tatgggtaat taaaacatca actccttcac
     2881 agataaaccg attatcctct gggaattcct ttctaaggtc attcccatct atgtttccat
     2941 aaacagctcg taatggttta atactagcta atctatctgt aacttctttc gatccaatat
     3001 cacctgcatg ccaaatttca tcacaagaat gaagatgttt caatatgcct tcatccaaat
     3061 acccatgtgt atcggatagt aaaccgattc tcatgagcga tgaaaatctt caattaataa
     3121 gcgtcgattg cagaaaaaat attcgggctc ttgatgattg gagctgacta aaatagtccg
     3181 actaccacca aattcctcta aaagagctcg ataccattca gaagcacgtg aatccaaatt
     3241 ggataatggt tcatccagta aaagcaatgg agtatcactc aaaatcgcca atatcagtcg
     3301 aacacgttgc ttcattccac tggagaaata actgattcgc ctatcagcag cgtgggtgag
     3361 acctgatttc tgaagtatct ccgattcgct aagattatta atcgatttct tgacggagaa
     3421 atggaaatga atcaactctg tcaacgtaaa ttcctctatt aattcaagat aaggtgctgc
     3481 aatactcaca tattggtaaa catgttcagg ttcgatttcc tgttcattaa ttcgccattg
     3541 aattttacct gctgaactta aaatagaacc ggcaactatc tgaagtaaag ttgattttcc
     3601 tgaaccattg gcacccaaaa acacacatcg atcacctgat gcaatatgcc aacttaaacc
     3661 gttaaatatc cagttgaagt tatagcgctt accaacctca ttaagagtaa tttccatctg
     3721 attgatggtt tctattcttc agatccgtaa ccccgaatta ttcctcttct tgcagcttga
     3781 acaaacagaa caatttnnnn nnnnnncaat ttcatctcgt tctgggctag caggcatgtc
     3841 ggtttcgatc ttctgaatgg ctttagttgt attcaatcca ctttggaata gtacccgata
     3901 gatgtcttga atttcactaa ttcgttcgtt agaaaagcct cttcgacgta aaccaatgga
     3961 attaatcccc acatatgaca tcggctctcg agctgcttta acaaaaggag gaatgtcttt
     4021 acgtaccaaa gaaccacccg aaaccataac gtgagctccg atatgcacaa actgatgtat
     4081 tgctgacata ccacttaaga tggcataatc gtcaactaca acatgcccag ccatattcac
     4141 agcattacct aaaatgcaat aattaccgat gatacaatca tgtgcaacgt gagcataagc
     4201 catgatattg gtatggtgcc caacgactgt tttcatccga tctgtagtac ctctgtttat
     4261 ggtggcacat tcacgaatgg tcgtataatc gcctatttcg acagtagtaa cttcaccatt
     4321 aaatttgagg tcctgaggaa ttgctgaaat gacagctcca ggaaaaattc ggcagtgttt
     4381 acctattctg gctccgtcca ttatggtaac gttaggacct atccaggtac cttcaccaat
     4441 agttacatca ccgtatattg ttacaaaagg actaattgta actccttcac cgattttcgc
     4501 atcgggatgt atggaagcaa gtgtttggtt cattcgtgta attcgaagcc aaatgtagtc
     4561 aaatgtgaat taatcgctaa gtcccttctc ttttacaatc tgagccatca tttcggcttc
     4621 tgtaacaggt ttattattga cgtatgcaac acctctcatg tgacaaatac ctctgcgaat
     4681 aggagaaatc aattcaagtt taaacactaa tgtaccacca ggaataacct tttgtttgaa
     4741 tttgacattg tcgatcttca tgaaataggt tagataattt tccggatctg gaacagtatt
     4801 caaaaccaaa attccaccgc actgagccat tgcttcaata atcaaaacac atgtggtggg
     4861 agtgaaaaac gtgacaatga acgaagattt cttccgtggt cactttcctg gaaatccagt
     4921 tatgccgggt gttttgatta ttgaagcaat ggctcagtgc ggtggaattt tggttttgaa
     4981 ta
//
LOCUS       DAOOQW010000179         4967 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_20324_length_4967_cov_4.255904, whole genome shotgun sequence.
ACCESSION   DAOOQW010000179 DAOOQW010000000
VERSION     DAOOQW010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4967
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20324_length_4967_cov_4.255904"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1091
                     /locus_tag="PK281_09310"
     CDS             <1..1091
                     /locus_tag="PK281_09310"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOY49579.1"
                     /translation="ITSNFTSMGANTFNIRNRGSNMRIGKGGKRPPVYKTISFEEASR
                     FKDEFRFPCKVGISCVGSRISTVKSGSLKTNPNITIFGGDENYLITAGYELSEGRNFS
                     MTEINTGSNVALIGQELKKNLFPKSSPIGKEISVGALKVKVIGLLKEKGSSFGFGGDK
                     IVLLPVLNVRQHFGSNSMSFVINVLADSPATIDATVAEATGFFRKIRKLRTKEPANFE
                     LTKSDNLSAMLIDNLSFVTIAATLIAIITLVGAAIGLMNIMLVSVTERTREIGVRKAI
                     GATSITIRNQFLIEAIVICQLGGILGIVLGILMGNGVSMYFDQGFIIPWAWMILAAIL
                     CFIVGLLSGLIPAIKAAKLDPIEALRFE"
     gene            complement(1094..2017)
                     /locus_tag="PK281_09315"
     CDS             complement(1094..2017)
                     /locus_tag="PK281_09315"
                     /inference="COORDINATES: protein motif:HMM:NF039709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6263 family protein"
                     /protein_id="HOY49580.1"
                     /translation="MFTRFFTRGIIGLSLLLAACSGGKEYDLKYRPTANTRFAFSNVT
                     TMHMDQKMMGQNMKMDQEMIYDGVYEIGAMGSDSSYDVTLTYTRIRVNQTMESPMKKE
                     TKMDSDNIDTTSSTEDQKMYTEILNVPMKMKISKTGKVMDLKGLSEAIKAVMSDSTGA
                     ISKEAEEMAGQFSDDNLKNSMEFMFALYPEKKVSPKDSWEIEQKYEAGYPMLIKQKYV
                     FEEEKEGKIILSNTGTIQTNKGGKGMMAMMAKMIELKGDQTGTIELDQKTGLPLMNSS
                     EMKITGEMNMMGVKFPIQMNTTSKVSLKALN"
     gene            complement(2043..3167)
                     /locus_tag="PK281_09320"
     CDS             complement(2043..3167)
                     /locus_tag="PK281_09320"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOY49581.1"
                     /translation="MIPSEKASAKPIWGIIRLVLSIFLFLYSLLTLIPLWWNEGILYN
                     LTALEDSGGICGMSLVLFLIPKHNLIPAYRFTRWLPLLAFLLLLQPAIRASREFHKLD
                     ESVTPGALKAFSWGRWIKGNKPPVASKRVEIQASDNKKLEGFVFHKNGLVGKRPLILI
                     LHGGGFTTGSAIHGAELAICLADHEWITASVDYRLAPETAYPGQIEDVKNWISYLRNH
                     ANELDIDTTKIFLAGESAGATIALNSAYTLHDPKIGAVANLYGITSADKSQAELWESI
                     TEPLDMLDAYRGSTSLDEINPVAQCSLGGIPTISIHGQKDPIVHWQHAQILSDALAKA
                     HIDQQLVLLPWATHLFNHPGFGPSGQLSVEYIDRFFRRHL"
     gene            complement(3164..3511)
                     /locus_tag="PK281_09325"
     CDS             complement(3164..3511)
                     /locus_tag="PK281_09325"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOY49582.1"
                     /translation="MKSVKIIFSACIFSIFLFSSCKDNELRMCFETDNNTPKVNERVN
                     FNATCSKGLDLYHWNFGDGRDTVTKVPNVQHTFDNAGYYTVTLHNTITQVVGECPPNG
                     NGNVASSTVEVTE"
     gene            complement(3621..4484)
                     /locus_tag="PK281_09330"
     CDS             complement(3621..4484)
                     /locus_tag="PK281_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HOY49583.1"
                     /translation="MKLEGKKNKYTVPLRSASTSSAAGQAQQSLDEYVVPEPVEGSTF
                     IYLCPKAPRMSHHHHEEDHLKSSAFISDIVIGMADGLTVPFALAAGLSGAAQSNSVVI
                     TAGIAEIVAGSIAMGLGGYLAGKTEQEHYASEWKREEEEVERIPEREKAEVREVFAEY
                     GLSEETQHLIADEMAKDKKKWIEFMMRFELGLEEPHVNRARNSAATIGIAYIVGGVIP
                     LSGYVVTDTPNSGLMISAILTVLCLFIFGYYKSKVTGQPAIKGALKVTFIGVFAAAAA
                     FFVARAFDQLL"
     gene            4495..>4967
                     /locus_tag="PK281_09335"
     CDS             4495..>4967
                     /locus_tag="PK281_09335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49584.1"
                     /translation="MQVHLSSPCKAGIAIPEKAFFCSSCQKQVHDIRNLEPEEIDKLI
                     LANKGELCGIARLEQVEFTHELSWKIFSRPVWKGMMASMMVYFQPNLSQAQAPVNPPQ
                     VEINAAGKCVNPAYRKAPVDTVSEAVTTTPPQTAIRKKRFYGIRLARIGKRTYYL"
BASE COUNT         1400 a         1020 c          971 g         1576 t
ORIGIN      
        1 gtattacttc caattttacc tcgatgggtg cgaatacgtt taacatccga aatcgtggat
       61 ctaacatgcg tattggaaaa ggcgggaagc gtcctcctgt ttataaaaca atctcgtttg
      121 aagaggcaag tcggtttaaa gacgaatttc ggttcccttg taaagttggt attagctgtg
      181 tgggatcgcg tatttcaact gtgaaatcgg gttcattaaa aacaaatccg aacattacaa
      241 tttttggtgg ggacgaaaac tacctcatca ctgcgggtta tgaattatcg gagggaagaa
      301 atttctctat gactgagatt aatactggtt ctaacgttgc actgattggt caagaactca
      361 agaaaaattt attccctaaa tcttctccta ttggaaaaga aatttcggtt ggtgcgctta
      421 aagtgaaagt gatcggatta ttgaaagaaa aaggctccag ctttggcttt ggaggagata
      481 aaattgtttt gttgccggta ttaaatgtgc gtcaacattt tggttcgaat tccatgtcat
      541 tcgttattaa tgtcttggct gattctcctg caaccattga tgcaactgta gcagaagcaa
      601 ccggattttt caggaaaatt cgaaaactca gaacgaaaga acccgccaat tttgaattaa
      661 cgaaaagcga taatctatcg gcgatgctga ttgataatct ttcgtttgtg accatcgcag
      721 ctacattaat agctattatc actttagtag gtgctgctat tggcttgatg aatattatgc
      781 ttgtttcggt gaccgaacgc acacgcgaaa ttggcgttcg taaagctatt ggtgcgactt
      841 caattacgat tcgaaatcaa tttttgattg aagccattgt tatttgccaa ttaggaggaa
      901 ttctaggaat tgtcctcgga atattaatgg gcaatggtgt ttcgatgtat ttcgatcaag
      961 gatttattat tccttgggct tggatgattt tggctgcaat cctttgtttt atcgtgggac
     1021 ttttatcggg tcttattccg gccattaaag cagcgaaact cgacccgatt gaagccttac
     1081 gttttgaata aaattagttg agtgctttca agctaacttt cgaagtggta ttcatttgaa
     1141 ttgggaattt tactcccatc atattcattt caccagtgat tttcatttct gagctattca
     1201 tcaatggcaa gcctgtcttt tgatctaact caatcgttcc ggtttgatct ccttttaatt
     1261 caatcatctt agccatcatt gccatcatgc cttttccgcc tttattggtt tgaattgtac
     1321 ctgtattgga taggatgatt tttccttctt tctcctcttc gaaaacatat ttctgtttaa
     1381 tcaacatggg ataaccagct tcatatttct gttcaatttc ccaagaatct tttggcgaaa
     1441 ccttcttctc cggatataaa gcaaacatga attccatgct gttttttaaa ttatcatccg
     1501 aaaattgtcc agccatttct tccgcttcct tagaaattgc acctgtacta tccgacataa
     1561 ctgcttttat agcttctgac aatcctttta aatccatcac cttcccagtt ttgctgattt
     1621 tcattttcat gggaacgttc agaatctccg tgtacatttt ttgatcttct gtacttgaag
     1681 tagtatcaat attgtcggaa tccattttag tttctttttt cattggagat tccattgttt
     1741 gatttactcg gatgcgtgta taagtaagcg ttacatcata cgaactatca cttcccattg
     1801 ctccaatctc atacacacca tcataaatca tctcctgatc cattttcata ttttgtccca
     1861 tcatcttctg gtccatatgc atggttgtga cgttactaaa agcaaagcgg gtatttgcag
     1921 ttggacgata ttttaaatcg tattcttttc cgcccgaaca agcagctaat aaaaggctaa
     1981 gcccaattat tcctcgtgtg aaaaaacgtg taaacatgtg tgttgattta ctgttggttt
     2041 agttataaat gtcgtcgaaa gaatcggtca atatattcaa ccgataattg ccccgaaggt
     2101 ccgaaaccgg gatgattaaa caagtgtgtt gcccaaggta atagcaccaa ttgttgatct
     2161 atatgtgctt ttgcaagcgc atctgacaat atctgagcat gttgccaatg aacaattgga
     2221 tctttttgtc cgtgaatgct gatagttgga attcctccca aactacattg agctaccgga
     2281 tttatctcat ccaaagaagt tgaaccacga tatgcatcta acatatctag cggttctgtt
     2341 atactttccc atagttcagc ttgggactta tcagccgaag taataccata taaattagca
     2401 accgctccga tttttgggtc atgtagtgta taagctgagt tgagtgctat cgttgctcct
     2461 gcggattcgc cagctaagaa aattttagtg gtatcaatat ccaactcatt agcgtgattc
     2521 cgtaagtagc taatccagtt ttttacatct tcaatttgtc ctggataggc tgtttcaggt
     2581 gccaatcgat aatcgacact agcagttatc cattcgtggt cggctaagca tatggctagt
     2641 tcggcaccgt ggatggcgct tccggtagta aatccaccac catgtaatat gagtataagc
     2701 ggacgtttac ctaccaatcc atttttgtga aagacaaatc cttccagttt tttgttgtcg
     2761 gatgcttgta tctcgactct tttagaggca acgggaggtt tgtttccttt gatccatctt
     2821 ccccaggaaa aagcttttag agcacctgga gtaacacttt catctaattt atgaaattca
     2881 cgagatgctc gaattgccgg ttgaagtagt aggaggaaag caagtaaagg caaccaacgg
     2941 gtaaaacgat aagcaggaat tagattatgc ttgggaatta gaaaaagtac caaactcatt
     3001 ccgcaaatcc caccactatc ctcgagagca gttagattat ataaaatacc ctcattccac
     3061 caaagcggaa tgagggtaag taaagaatac agaaatagaa atatagaaag cactaaccga
     3121 atgatacccc atatcggttt agcagaagct ttctcggatg gaatcattca gtaacttcca
     3181 ctgtcgatga agctacattc ccatttccat ttggagggca ttcacccaca acttgagtta
     3241 ttgtattgtg aagtgttaca gtataataac cagcattgtc gaatgtatgc tgaacattag
     3301 gaacttttgt tacggtgtcg cgaccatcac caaaattcca atggtataga tctaatcctt
     3361 tgctacatgt tgcattgaaa ttaacacgct cgtttacttt cggagtatta ttatcggttt
     3421 cgaagcacat gcgaagctca ttgtctttac atgaactaaa cagaaatata ctgaaaatac
     3481 aggctgagaa gatgattttt actgatttca tgtgagggta cttgattgat tatgggtgaa
     3541 ttgagctgaa cgaatttagt tgaactgttt taattacaca acggtttttc aaacgaacta
     3601 ataattcatt tcgtatgtgc tcataataat tgatcaaatg ctcgagctac aaagaaagct
     3661 gccgcagccg caaaaacgcc tatgaacgta actttcagcg caccttttat ggccggttgt
     3721 ccggtaactt tactcttata atatccgaaa ataaataagc acaaaacggt aagaatagcc
     3781 gaaatcatca atcccgaatt gggtgtatcg gtaactacat atccactaag cggaattact
     3841 cctcctacaa tgtaggctat tccaatagtt gccgcactgt ttcgagcacg attgacatgt
     3901 ggttcttcta atcccaattc gaaacgcatc ataaattcga tccacttctt tttatcctta
     3961 gccatttcat cggctataag atgttgggtt tcttcgctca aaccatattc ggcaaagact
     4021 tcccgcactt ctgctttttc tcgttcagga atgcgctcca cttcttcttc ttcccgtttc
     4081 cattcggacg cataatgctc ctgttctgtc ttcccagcta aataacctcc caaacccatt
     4141 gctatactac cagctacaat ttcggcaatc cctgcagtta taacaaccga attgctttga
     4201 gctgcaccac ttaatccagc tgctaaagca aaggggacag ttaaaccatc agccattcca
     4261 attacgatat cggagataaa tgccgagctt tttaagtgat cttcctcgtg gtgatggtgc
     4321 gacatgcggg gagctttcgg gcaaagataa ataaatgtgc ttccttcgac aggctcagga
     4381 accacatatt cgtccaacga ctgctgagct tgtccggccg ctgagcttgt cgaagcggat
     4441 cgaagcggga ctgtatattt atttttctta ccttccagtt tcaatcggaa tattatgcag
     4501 gtacatcttt cctctccttg taaagcggga attgctattc ctgaaaaggc tttcttctgc
     4561 agctcttgtc aaaaacaggt gcacgatatt cggaatttgg aaccggaaga aatagataaa
     4621 ctgatactgg cgaataaagg agaattgtgc ggaatagctc gattggaaca ggtcgaattt
     4681 acacatgagc tatcttggaa aatattctct cgtccggttt ggaaagggat gatggcttcg
     4741 atgatggtgt attttcaacc taatttatct caggcacaag cacccgtaaa tccacctcaa
     4801 gtggaaatta atgctgctgg aaaatgtgta aatcctgcat atcgtaaagc gcctgttgat
     4861 actgtttcgg aagctgttac taccacacca ccacaaactg caattaggaa aaagcggttt
     4921 tatggaatcc gcctggccag aatcggaaaa cggacgtatt acctcaa
//
LOCUS       DAOOQW010000180         4923 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_20539_length_4923_cov_6.254108, whole genome shotgun sequence.
ACCESSION   DAOOQW010000180 DAOOQW010000000
VERSION     DAOOQW010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4923
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20539_length_4923_cov_6.254108"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1068
                     /locus_tag="PK281_09340"
     CDS             <1..1068
                     /locus_tag="PK281_09340"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY49585.1"
                     /translation="KIYRINMSTGVSNVMAQSLVQSPNGIVYDQAHNRLVFVNWGSNA
                     PIKALSLTDSVVTTIATTTLGNCDGLAWNGLDTWYIAAWTGQKIVKYDRDFANAPITL
                     ATNMSSPADIYYNQITDTLAIPNSGNNTVAFLGFPAIQNVACSLLPLNTAADQAIFEG
                     TSASFGDSVLRFNLTNTSGLGFAYPLARITPLSALPAGMDFGTSGNGFQVFASAWNPD
                     SVVEASFFFTVTQAIPENTMLDFKLDITNLFPSNADTCFFTDTFHVNLNPGTILGIEA
                     LQQALTVYPNPSNGIIQIELPNNQGNLSLYDVQGRLVRYLTKAPKSLQLGDETFDHAG
                     IYLLRWESNGQIIQQKLIRTR"
     gene            complement(1382..1609)
                     /locus_tag="PK281_09345"
     CDS             complement(1382..1609)
                     /locus_tag="PK281_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013598005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HOY49586.1"
                     /translation="MVRITQQRIKCIGCNACVEVAKYRWRLSTKDGKCTLIGGVEKKG
                     WYSVLVGDDELELNQLAARNCPVNIIRVEQV"
     gene            complement(1609..2847)
                     /locus_tag="PK281_09350"
     CDS             complement(1609..2847)
                     /locus_tag="PK281_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016420309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HOY49587.1"
                     /translation="MTQTKMELMAPVGSYESLMAAIQAGANSVYFGVEQLNMRTRSSI
                     NFTLEDLAEISRICTEHSVKTYLTINTIIYDHDISLMKKIVDAAFANNISAVIASDHA
                     VMNYAKKIGMRVHISTQANVTNIDTIEFYSAYADVMVLARELGLMQVEAISKEIARRD
                     VRGPSGDLVKIEIFGHGALCMAVSGKCYLSLHSNFASANRGACLQNCRHAYVVTDKET
                     GNELEIDNEYIMSAKDLCTIGFLDKVMESGVSVLKIEGRGRSADYVHTVITCYREAID
                     SIYDGSYSREKVEAWTYRLATVYNRGFWDGYYLGRTMGEWNDEYGSKATKRKIFIGKG
                     LKYYDAVKVGEFQLESHSLEVGDEILITGPTTGVIQQIVEELRLDNVKTDKVKKGDVF
                     SVSVEAKVRPSDKLYKIVDA"
     gene            complement(2991..4859)
                     /locus_tag="PK281_09355"
     CDS             complement(2991..4859)
                     /locus_tag="PK281_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015023500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOY49588.1"
                     /translation="MIAFLHLFLYIPLLGFLISLFLPDKNETLISRTAFFTVGIHLAV
                     LMVFLVIWLLGGHPRLNLRDIALYTADDYEFYLDFSFDRITAVYLFVGAILTFMVTIY
                     SRYYLHREKGYKRFFNTTLFFYLGYNLTILSGNLETLFIGWEMLGISSFLLIAFYRDR
                     YLPVKNAVKVFSIYRIGDVGIILAMWMSHHLWHENITFAKLSDSHMVSAHLQEHSWIG
                     VLISLAFLLTAAAKSAQLPFSSWLPRAMEGPTPSSAIFYGSLSVHLGVFLLLRTFPFW
                     EHQFSVRILIALLGLSTSIVASLIARVQSSVKSQIAYSSIAQIGLIFIEIAAGFENLA
                     LFHFAGNAFLRTYQLLVSPSVVSYMIREQFYNFVPKQHTVEDSLPKKIEYTLYMLSLK
                     EWNLDSFMYRRLWNPMKLIGKKLDFMSVNRVLVFFVIVYGIGLYTVYHRDFLSENVQH
                     YLPLVFSLFGVIMVLKSFSERIRVKLSWLLIIMNHFWVALAISFNEKFKFEQVHLYLS
                     GIVVMGFVGYLCLRRLKKLEHSIDLDQFHGHSYKHPKIAFVFLIACLGLTGFPITPTF
                     LGEDLIFSHIHSDQYLLASFTALSFVVDGLSIIRIFARVFLGPHSKSPTEMAYRSS"
BASE COUNT         1580 a         1122 c          887 g         1334 t
ORIGIN      
        1 aaaatttacc gcatcaatat gagcaccggt gtatcgaatg tgatggctca aagtcttgta
       61 caaagtccaa acggtatcgt ttacgaccaa gctcacaatc gtttggtatt tgtcaattgg
      121 ggaagtaatg ctcccatcaa agcactttca ttaaccgact ctgtggtgac aactattgcc
      181 accaccacac tcggcaactg cgatggacta gcttggaatg gtctcgatac ttggtatatc
      241 gcagcatgga ccggacaaaa aattgtgaag tacgatcgtg attttgcgaa tgccccaatc
      301 acgttggcaa caaacatgtc gagtccggca gatatttatt acaaccaaat caccgataca
      361 ctcgccattc cgaattcggg aaataacaca gttgctttct tgggatttcc agccatacaa
      421 aatgtagcat gctcactttt accactcaac actgctgctg atcaagctat tttcgaagga
      481 acttctgctt cttttggtga ttcggttcta cgtttcaact taaccaatac ttccgggtta
      541 ggatttgcct atccactggc gcgtattaca ccattatcag cacttccagc aggtatggat
      601 ttcggaacga gtggtaacgg ttttcaggtt tttgcttctg cttggaatcc agattcggtg
      661 gttgaagcca gctttttctt tacagttact caagctattc ccgaaaacac catgctcgat
      721 tttaagctcg acattaccaa cctatttcct tctaatgccg acacttgctt tttcacagac
      781 acttttcatg tcaacctaaa tcccggtact attttaggaa ttgaagcact tcaacaagca
      841 ttaaccgttt atccaaatcc ttcaaacgga atcatacaaa ttgaactgcc caataaccaa
      901 ggaaacttga gcttatatga tgtgcaaggt cgtttagttc gctacctgac taaagcacct
      961 aaaagtcttc aattaggcga cgaaaccttt gatcatgcag gtatttactt gcttcgttgg
     1021 gaatcgaacg gacaaatcat ccaacaaaaa ctcattcgta ctcgatagat tatttatttg
     1081 ggcagtgccc gggctattca ttacaaggca attttcgatc tgatttttta gtataaacta
     1141 aaagctcgtt tcgaaaattg ccttttcatt gctatcccta ctgcttattg gtgggagcaa
     1201 ttttgcggta ggtaaaaatc attttattaa tctgtatcat taatcctcgg actaaagtcc
     1261 gaggctaccg aagggaaact aatgcattgt caaacctgtt ccatccgagg ctgccgaagg
     1321 gaaactaatg cattgtcaaa cctgttccat ccgaggctgc cgaagggaaa ctaatgcatg
     1381 gtcaaacttg ttccacacga ataatattca ccgggcaatt tcgtgctgct aattggttca
     1441 attccaattc atcatctccc actaaaaccg aataccaacc tttcttctcc actccaccaa
     1501 tcaaggtaca ttttccatct ttggttgata atcgccaacg gtattttgct acttctacac
     1561 aggcattaca accaatgcat ttgatacgtt gctgtgtgat acgtaccatc aggcatccac
     1621 aattttatac agcttatccg aaggacgaac tttagcttct accgaaaccg aaaatacatc
     1681 acctttcttc actttatcgg ttttaacatt atccaacctc aactcttcaa caatttgctg
     1741 gataactccg gtagtcggac ctgtgatcaa tatttcatcg cccacttcca aggaatgtga
     1801 ttccaattga aactcaccca cttttactgc atcatagtac ttcaaacctt taccaataaa
     1861 gattttgcgc tttgttgctt tcgaaccata ttcatcgttc cactctccca tggtacgacc
     1921 taaataatag ccatcccaaa aaccacgatt gtaaactgtt gcaagtcgat aagtccacgc
     1981 ttctactttt tcacgagaat aactgccatc ataaatcgaa tcaatcgctt cacggtaaca
     2041 agtaattaca gtatgaacat aatcggcact tcgtccacga ccttcgattt tcaaaacgct
     2101 gacacctgat tccattactt tatcaaggaa tccaatcgtg cataaatcct tcgccgacat
     2161 gatgtattcg ttgtcgattt ctagctcgtt accggtttct ttatcggtca ctacataagc
     2221 atgacgacaa ttttgcaaac acgcaccacg attagcagaa gcgaaattcg agtgtaagct
     2281 caaatagcac ttacccgaaa ctgccatgca taaagcacca tgaccaaaaa tttcgatttt
     2341 caccaaatca cccgatggac cgcgcacatc acggcgagct atttctttac taatcgcttc
     2401 cacttgcatc aagcctaact cacgcgccaa aaccatcaca tccgcatagg ccgaatagaa
     2461 ctcgatcgta tcgatattgg tcacattagc ttgcgtagaa atatgcaccc gcattccaat
     2521 ttttttggca taattcatca cagcatgatc cgatgcaata acagccgaaa tattgttggc
     2581 aaaagcagcg tctacaatct tcttcatcaa ggagatgtcg tgatcgtaga taatggtgtt
     2641 aatcgtgagg tatgttttaa ccgaatgttc cgtacaaatt cgagatattt cagctaaatc
     2701 ttctaatgtg aaattgattg aagagcgcgt gcgcatgttc aattgctcaa ccccgaaata
     2761 aaccgagttt gcgcccgctt gaatagcagc catgagtgat tcatacgaac caactggcgc
     2821 cattagctcc attttagttt gtgtcattgc gtagtttgaa aaaggacttt aggaatccta
     2881 gtgcaaaaat aaaatattcc ccgcaaccct agattataat cgattaacct agatttttat
     2941 tcccagcaac cccggatttt aattatagcc ccggatttta atccggggaa ttaggatgaa
     3001 cgatacgcca tctctgttgg agatttcgaa tgcggcccca aaaacacccg agcaaaaatt
     3061 cggattatcg acaagccatc caccacaaaa ctcaaagccg taaaagaggc caaaagatat
     3121 tgatcggaat gtatgtgaga gaaaatcaaa tcctctccca gaaacgttgg agtgatagga
     3181 aaacctgtca atcccaagca agcgataagg aacacaaagg caattttggg atgtttgtag
     3241 gaatgaccat gaaactgatc taagtcgata ctatgctcca attttttaag tcggcgcaga
     3301 cagagataac caacaaaacc cattaccaca ataccactca aatacaaatg cacctgctcg
     3361 aatttgaatt tctcattaaa cgaaatagcc agagctaccc aaaagtgatt catgataatc
     3421 aacaaccagc tcagcttcac acgtattcgt tccgagaatg acttcaacac cataattacc
     3481 ccaaacaagg aaaatacaag tggtaaataa tgttgaacgt tttcggataa aaaatctcga
     3541 tgataaacgg tgtaaagccc tatgccatat acgataacaa agaatactaa gacccgatta
     3601 acactcataa aatcgagttt tttaccaatg agtttcatcg gattccacag gcggcgatac
     3661 atgaacgaat cgagattcca ttcttttaaa ctaagcatgt acaaggtata ctcgattttt
     3721 ttcggcagac tgtcttccac cgtgtgctgt ttaggaacga aattgtaaaa ctgctcccga
     3781 atcatgtaac tcactaccga aggagaaact aacagctgat aggttctcaa gaatgcattt
     3841 ccggcaaaat ggaaaagcgc caaattctca aatccagcag caatttcgat aaagattaat
     3901 ccaatttgtg cgatcgatga atacgcaatc tgactcttga cagaagactg aacacgagca
     3961 attaaagaag ctacaatact ggttgataat cctaataaag caattagaat tcgtaccgaa
     4021 aattggtgtt cccaaaatgg aaaagttcga agcagaagaa acacacctaa atgcaccgaa
     4081 agtgaaccgt aaaaaatggc actcgaaggt gttggacctt ccatagcacg tggcaaccaa
     4141 gatgagaatg gcaattgagc cgattttgca gctgccgtaa gtaagaatgc taatgaaatg
     4201 agaactccaa tccaactatg ttcttgcaaa tgcgcactaa ccatgtgcga atcgctcagt
     4261 ttagcaaagg tgatgttctc atgccacagg tgatgactca tccacatagc cagaataatt
     4321 cccacatctc caattcggta aatagaaaat actttaacag cattctttac tggtagataa
     4381 cgatcacgat aaaatgcaat cagcaggaat gaggatattc ctagcatttc ccaaccaata
     4441 aaaagcgttt caagatttcc cgaaagaatg gtcaaattgt atccgagata aaagaacaaa
     4501 gtggtattaa agaaacgctt gtagcccttt tcacggtgca aataatagcg actgtaaatc
     4561 gtcaccataa aagtgagtat agcacctaca aacagataaa cagctgtaat tcgatcaaaa
     4621 ctaaaatcga gataaaactc ataatcatcg gctgtgtaaa gagctatatc gcgcagattt
     4681 aatcgtggat gtccgcctaa taaccatatc accagaaata ccattaacac ggcaagatga
     4741 attcctacgg tgaaaaaagc tgtccgagaa atgagtgttt cgtttttatc gggcaagaac
     4801 aagctgatca aaaatcccaa taaagggatg tagagaaata aatgaaggaa tgcgatcatg
     4861 attctgaaat aaggtaaacc ggaagattct cctcgtgcgt ttcaaacaca ggttcgagat
     4921 tag
//
LOCUS       DAOOQW010000181         4917 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_20573_length_4917_cov_4.570547, whole genome shotgun sequence.
ACCESSION   DAOOQW010000181 DAOOQW010000000
VERSION     DAOOQW010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4917
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20573_length_4917_cov_4.570547"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1077)
                     /gene="rmuC"
                     /locus_tag="PK281_09360"
     CDS             complement(<1..1077)
                     /gene="rmuC"
                     /locus_tag="PK281_09360"
                     /inference="COORDINATES: protein motif:HMM:NF014680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HOY49589.1"
                     /translation="MEIVFLLGGLALGALLVFLWSKSQLAQSQQANLQNTVKVQSELA
                     SAQQLLNSKEDEVLRLRKEAESLREQHMQSSAQAALWKSSFDSLKERMDEQKQEVEHL
                     RERFNLEFRNIANELLEDKSKRFTETNKENIDLILKPLKEKIAEFEKTVDTTHKASLQ
                     DFSALREQIGSLKELNQQMSKDAINLTKALKGETKTQGNWGEFILESVLEKSGLTKGR
                     EYVVQESHNDEEGRRFQPDVIIRLPEGKSIVIDSKVSLIGYEKMVSADDETQRLIAGK
                     EHLASLRAHIRGLSEKNYQQLHSLKSLDFVLLFVPIEPAFAAAVQLDQALFNEAFDKN
                     IVVVSPTTLLATLRTIASIWRQEYQ"
     assembly_gap    1164..1263
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1374..1473
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1988..3745)
                     /locus_tag="PK281_09365"
     CDS             complement(1988..3745)
                     /locus_tag="PK281_09365"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOY49590.1"
                     /translation="MELALIMRIGFFILCLLSISLQAQTYRLVAIEMPANKAFRQAPK
                     SSFNSEEQRKIAINTFVSNLQADGYLTASLDSSSIQKDSLSRDNTLTAYIHKGELYTW
                     AKLHSINVDEEALSSSGFREKMYRNRPFRYTQTRRMMRKVITYYENNGYPFVSLSFDS
                     LKTGSTHIEAGLKVNKGELFHVDSVTIRGTARITPRYLYNYIGVKPGDVYNEAVMRQI
                     STRMKELPFLTETRPADVFLLENSALVRLYLNDRKASSFNGIVGILPNTSNNGKLLLT
                     GEVNLKLKNSLGRGELIDAEWRRLQQGTQSLNIKLGWPFLFNTRVGVEGSFGLYKRDT
                     SFINVQQNLGLQYLMKGTDYLKAFYENRSSNLLSTKGLENILTLPDYADIRANLYGIE
                     ANLTRLDYRLNPRKGYRILARLGTGTRNIQKNPALKPELYDQLTLRSVQYSGHVDADF
                     YFPIRNRSTINLGVLASVINGSSIFENELFRIGGNNTLRGFNEESIFASTYSIVNLEY
                     RYLLEENSFLFAFWNGAYYENKAVNRNVVDRPFGFGAGMSFETKAGIFSISYALGKQF
                     DNPVEFKSAKVHFGITSLF"
     gene            complement(3730..4416)
                     /gene="ung"
                     /locus_tag="PK281_09370"
     CDS             complement(3730..4416)
                     /gene="ung"
                     /locus_tag="PK281_09370"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOY49591.1"
                     /translation="MESIVVQPKIGESWKKALGDEFQKPYFAKLKEFLVAERAEATIY
                     PPGGKIFAAFDHTPFDKVKVVIMGQDPYHGPGQANGMSFSVSPGVKIPPSLMNMFKEI
                     REDLKVEVGREGDLTRWADQGVLLLNATLTVRAASPASHQGKGWEQFTDQVIRLLSEK
                     RTGLVFLLWGRPAQLKEKLIDTSKHFVLKAAHPSPLSAYNGFFGCKHFSYTNKILLDQ
                     GLTPIDWSLP"
BASE COUNT         1374 a         1084 c          822 g         1437 t
ORIGIN      
        1 ctgatactct tgacgccaaa tacttgcaat cgtgcgtaaa gtagccagta aagtagttgg
       61 actaactacc acaatattct tatcaaatgc ttcattaaat aaagcttgat ctaattgcac
      121 ggctgcagca aatgcgggtt caataggaac aaacagcaat acaaaatcga gactttttaa
      181 agaatgcaat tgctggtaat tcttttccga caatcctcta atatgcgctc tgagcgaagc
      241 caaatgctct ttcccggcaa taagtcgctg tgtttcgtca tcggcactca ccattttctc
      301 gtaaccgatt aaggaaactt tcgaatcaat tacaatcgat tttccttctg gcaaacgaat
      361 gatcacatca ggctgaaacc tccgtccttc ctcatcatta tgactttctt gcacgacata
      421 ttctcgccct ttcgtcaatc ccgacttttc taaaaccgat tccaaaataa actcacccca
      481 atttccctga gttttcgtct ctccttttag agctttagtg agatttatcg cgtctttact
      541 catttgctga ttgagttctt tcaaactacc aatctgctcg cgcaaagctg aaaaatcctg
      601 caaagaagct ttatgggtgg tatcgaccgt tttttcaaat tcggcaatct tttccttgag
      661 cggtttcagg attaaatcga tgttttcctt gttggtttcg gtaaagcgct tcgatttatc
      721 ttctagcaat tcgttagcaa tattgcgaaa ctccaagttg aaacgttcac gtaaatgctc
      781 tacctcttgc ttttgctcat ccatccgctc tttcaacgaa tcaaaactac ttttccatag
      841 cgccgcttga gcactcgatt gcatgtgttg ttcacgtaat gattcagctt cctttctaag
      901 tcggagcact tcatcttctt tgctattcaa tagctgttga gcagacgcaa gttccgactg
      961 taccttaaca gtattctgca aattagcctg ctgactttga gccaattgcg acttcgacca
     1021 aagaaaaaca agcaaggcac caagagctaa accgccaagt agaaaaacaa tctccatgtg
     1081 aaaatttcag gtcctgcaaa ttacgcgatt ctattcccag tatttcgggt tcggctattt
     1141 atcagaaaat gtttttgggc gtgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnttctatt cccagtattt cgggttcggc tatttatcag aaaatgtttt tgggcgtgtc
     1321 cttcggtcgc attttcagtt tcaacttccc tcgtatgcca tagccttggc gaannnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnggcatat tcaacttccc tcgtatgcca
     1501 tagccttggc gaaggcatat tcaacttctc tcatatgcca tagccttggc gaaggcatat
     1561 tcaacttccc tcgtatgcca tagccttggc gaaggcatat tcaacttccc tcatatgcca
     1621 tagccttagc gaaggcatat tccatttcaa attcattcat atgccatagc cttggcgaag
     1681 gcaaattcaa ccactcacgc atcatgctac tcaacttatc attaagttaa gcacaaactt
     1741 atcaactaca ctgttcaaaa acagtcgttt ataaacgttt aacagacgaa tcatgtgctc
     1801 gggattttgt ttctttcagc cggtgggtgg ttagtggtcg gccccgcgtg cgtgcgagga
     1861 taaaaagatg aatttcaatt tattacatga atcataatcg caggcgtatt catcatttaa
     1921 gaagaatcaa cctcaattta tagacttgaa aaatgcatat tccagattca agcttaatcc
     1981 aatctactta aaataaacta gttataccaa aatgtacttt ggccgactta aactcaacag
     2041 gattgtcgaa ctgtttaccc aaagcgtatg agatggagaa aatccctgcc ttagtctcga
     2101 aactcattcc cgcaccaaag ccaaacggcc tatcaaccac atttcgattc actgctttat
     2161 tctcgtaata cgccccattc caaaaggcaa ataggaatga attctcttct aataaatatc
     2221 gatactccaa attaacaatt gagtaagtag aggcaaaaat cgattcctca ttaaatcctc
     2281 gcagtgtatt atttcctcct attcgaaaca gctcattttc gaaaatactc gagccattga
     2341 tcacgctcgc taatactccc aaattaatcg tagatcggtt acgaattgga aagtaaaaat
     2401 cagcatccac atgtccgcta tactgcaccg aacgtaaagt aagctgatcg tataactcag
     2461 gtttcaatgc aggattcttt tgaatgttgc gtgttccggt acctaatcga gctaaaatcc
     2521 gatatccttt tcgtggattg agtcgataat cgagtcgggt gagatttgct tcaataccat
     2581 acaaatttgc acggatatcg gcataatcgg ggagagtcag aatattctcc aagcctttag
     2641 tagatagcag attagaactt cgattctcgt aaaatgcttt gaggtaatcg gttcctttca
     2701 tcaaatactg caagcccaaa ttctgctgaa cattaatgaa actggtgtcg cgcttataca
     2761 atccaaatga gccctcaacc ccaacgcggg tattaaacaa aaagggccag cctagcttaa
     2821 tattcaaact ctgcgtccct tgttgaagtc gccgccattc cgcatcaatc agttcaccac
     2881 gccccaaact gtttttcagt ttcaagttaa cctcacctgt tagaagtaat tttccattat
     2941 tagaggtatt cggcagtatc cccacaatcc cattgaaact actcgctttc cgatcattca
     3001 aataaagcct caccaaagca gaattctcca ataaaaaaac atctgcagga cgtgtttcag
     3061 tcaagaaagg taactccttc atacgcgtcg aaatctgacg cataaccgct tcattgtata
     3121 catcacccgg cttaacacca atataattat acagatagcg aggtgtgatg cgggcagttc
     3181 ctcgtatcgt tacactatcc acatgaaaca attcaccctt atttaccttc aaaccagcct
     3241 caatatgagt tgagccggtt ttcaagctat caaagctcaa actcacaaaa ggatatccgt
     3301 tattctcata ataggtaatg actttacgca tcatccggcg tgtttgagta tatcgaaatg
     3361 gtctattccg atacatcttt tctcgaaaac cactactact tagcgcttcc tcatctacat
     3421 taatggaatg caattttgcc cacgtgtata attcaccttt gtggatgtac gctgttagtg
     3481 tattatcacg acttaatgag tctttttgta tagaactact atccaaacta gctgtcagat
     3541 aaccatcagc ttgtaaattg cttacaaacg tattgatggc aatttttcgt tgttcttcag
     3601 aattaaatga cgattttggt gcttgtcgga atgctttatt tgctggcatt tcaatagcta
     3661 ccaatcgata cgtttgcgcc tgtagcgata tcgagagtaa gcataaaatg aaaaagccaa
     3721 tccgcataat taaggcaagc tccaatcgat aggtgttaaa ccttgatcga gaagaatttt
     3781 attggtataa ctaaagtgct tacatccaaa gaatccatta taagcagata atggtgaagg
     3841 atgtgcagct ttaagaacaa aatgtttgct tgtatcgatg agtttttctt tgagttgagc
     3901 gggtcttccc caaagtaaaa aaaccaagcc cgttcttttt tcggatagca aacgaatcac
     3961 ctgatcggta aattgttccc aaccttttcc ttgatgagaa gcaggtgaag ctgcccgaac
     4021 tgttaatgtt gcgttaagta acaacactcc ttgatcagcc cagcgtgtta aatcgccttc
     4081 tcgtcctact tctaccttta aatcttcccg aatttccttg aacatattca tcaaagaagg
     4141 tggtattttc actcctggcg aaaccgaaaa actcattcca tttgcctgcc ctggtccatg
     4201 atagggatct tgtcccatga tgaccacttt tactttatcg aaaggtgtat gatcgaaagc
     4261 agcaaatatt ttcccgccag gtggatatat ggtagcctca gcacgttctg ccactaaaaa
     4321 ctctttcaac ttggcgaaat agggcttttg aaactcatct cccaaagctt tcttccagct
     4381 ttcaccaatt ttaggctgaa ctacgatact ttccatgaat tattctttcc tgcaaacata
     4441 cgtattcgcc ttgccaccac gtttcattgt ggaaaccttt cattttttat cccgtaacta
     4501 atcagaattg cccgctcggg aagaaaaatg gactaaaata aatatccatg cagcttgtta
     4561 aaaacttctt gattctctgc ttcgccttga tgctgttgtc tttcacagct tgtaaatcgc
     4621 atgtcgactg tccagcttac ggtaaaaacg tctcacatca taaagaagtc aggagctaat
     4681 agttaattgc ttatttttgc cctgaataaa aaagagatga gtcgaccaaa atttgtttta
     4741 gtagccgtat tatttattgg tacaagcttg atgatgtctt gcagatcaag aaagcatgtt
     4801 gattgccctg cgtacggcaa aaacacagta caatcagtaa ataaacaatc ctgattatat
     4861 gttgaattgg ctcccattag aaacggagca acaattagat tctatcgttc aactatc
//
LOCUS       DAOOQW010000182         4909 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_20615_length_4909_cov_5.757520, whole genome shotgun sequence.
ACCESSION   DAOOQW010000182 DAOOQW010000000
VERSION     DAOOQW010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4909
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20615_length_4909_cov_5.757520"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..911)
                     /locus_tag="PK281_09375"
     CDS             complement(<1..911)
                     /locus_tag="PK281_09375"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOY49592.1"
                     /translation="MTQEFYLLPLIPVVLLILFSALVSLDKLVYFILFFTPFSLTVEF
                     NEYAALTLPTEPLLFGVMLVFFFKLLFEGGFDRRITKHPVTIAIIVMLLWMSITSLTS
                     TMPLVSIKFTIARMWFVVSFYFVASQVFRKKENIRFWIWLFTIPLSIVIIYSIIQFFH
                     YNIDKNALYWVMQPFFKDHTIYGAVIAMMLPVMFVFAFDKSYSPKARFTSLIFFFIIF
                     IGIIVSYTRAAWLSIAGAYVVYLIFLLKVNWRVVFSGFLVVAAVLFYYQDNIMQKISS
                     NKQSSSQDLAKHLKSVSNIKNDASNLER"
     gene            complement(994..2019)
                     /locus_tag="PK281_09380"
     CDS             complement(994..2019)
                     /locus_tag="PK281_09380"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HOY49593.1"
                     /translation="MESTQPGSLFDSTNLLIFLWKKRKPILIATLAALIASVVFSSEF
                     FIKPRFKSSVIMFPTTNSSISKSLLSENSFEKENILQFGEEEQVEQMLQILNSDEIRE
                     RLISKYNLMEHYRIDTDGRYPMTELNKKFEENISFNRTEYLSVRVDVMDEDPKLAADI
                     ANEICTLLDTVKTRMQHERASQALAIVQKEYFDFTHYLQAREDTLNKLRDLGVLDYDA
                     QVERLSEAYGKALMAGSASATRSIDEKMAILAKYGGLYTSITQDMEHDRESLSRLKTK
                     YEEAKVDATDKIPHKFVVNLAKPAEKKSYPVRWLIVLVSTVATFLLAVVLTIIIENIQ
                     RIRSRFE"
     gene            complement(2085..3524)
                     /locus_tag="PK281_09385"
     CDS             complement(2085..3524)
                     /locus_tag="PK281_09385"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOY49594.1"
                     /translation="MQKKFIQNLALLLTLNLLIKPFWILGIDRAVQQTVGTEAYGMYF
                     AIFNFSFLLNILLDFGITNFNNKNIAQNNHLINKHFSSILALKLLLGIVFMVVTLAAG
                     FLIGYSPQQLGLLAMLGFNQFLAFMILYMRSNLAGLHLFKTDSIISVLDRSLMILFCS
                     ILLWGHVTEGPFQIEWFIYTQTFSYLLTTLITFFIVVRKAAFKRLKWNRPFFFLILKK
                     SFPFAILVLLMTFYNRVDTVMLERILPEGATESGIYASAYRLLDSANMISFLFAGLLL
                     PMFARMLRYKESVEQLVKLAFTILLTPAIVITIGCIFYNEELMVLMCKEHALESAQVF
                     SILMGCFIASSLSYIFGTLLTANGNLLQLNIMAASGMVFNLTLNYILIPHFKAQGAAL
                     SSLITQFVTALLQIYLAQRIFKFKVNTRLMVQLSIFIPCVVILAWVSQTLFQNWILGM
                     VVFSGSSAAFAFSIGLLHVKSIFRILKYG"
     gene            complement(3539..3997)
                     /locus_tag="PK281_09390"
     CDS             complement(3539..3997)
                     /locus_tag="PK281_09390"
                     /inference="COORDINATES: protein motif:HMM:NF025491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4293 domain-containing protein"
                     /protein_id="HOY49595.1"
                     /translation="MIQRIQSLYLFLAAVCSGVLLAAPLYNTTVEDKSYTVYLGGLIQ
                     GEEVIQSQPSLVAVGIILMIFPLIILFLFKKRALQLRLTASLMLANTAMLLLLIATIN
                     KTLEKISGTVSEGTYGWGLALPLIGVLFIFLANRAIRKDDALVRSADRLR"
     gene            4221..>4909
                     /locus_tag="PK281_09395"
     CDS             4221..>4909
                     /locus_tag="PK281_09395"
                     /inference="COORDINATES: protein motif:HMM:NF019139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HOY49596.1"
                     /translation="MYDILELNAKLVGELREIALKLNIPKAEALKKQDLVYRILDEQA
                     LNPTEASRLKKKEESAKAANKPAADVTPKKNVETVRITLDPAEVRKLASQAIRDEKTP
                     AEKTPVAKKAAEEKPAPTEVKPLRGRRPNASKVPPAVLPEKVEEPAAQTPSPTPAPSP
                     KPHVRQPHPIKRTDVAIPNSELASEKPNTPPPAEGEVVRNVPTPHPDGKIIRNERRDR
                     TNRSLFDKRSD"
BASE COUNT         1675 a         1027 c          940 g         1267 t
ORIGIN      
        1 cgttccaaat tggaagcgtc attcttaata tttgatactg attttaagtg ctttgctaaa
       61 tcctgagacg agctctgttt gttgctcgaa atcttctgca tgatgttgtc ctgataataa
      121 aacaagactg ctgcaacgac taaaaaacca ctgaaaacta ctctccagtt tactttcaaa
      181 aggaatatta agtaaacaac ataagcacca gcaatactga gccaagcagc gcgtgtatag
      241 gaaacgatga tgccaataaa gatgatgaaa aagaaaatca gagacgtaaa tcgtgctttg
      301 ggagaatacg atttatcaaa ggcaaatacg aacatcaccg gaagcatcat cgcaatcaca
      361 gctccgtata tggtgtgatc tttaaagaat ggttgcatca cccaataaag ggcattttta
      421 tcgatgttgt aatggaaaaa ctggatgatg gaatagatga taacgatact gagtggaatc
      481 gtaaaaagcc aaatccagaa acgaatgttc tcttttttgc gaaatacctg gctggcaacg
      541 aaataaaacg aaaccacgaa ccacatacgt gcaatggtaa acttgatcga gaccaaaggc
      601 atggtgctcg ttaaggatgt gatggacatc caaagaagca tcacaattat ggcaatggtt
      661 accggatgtt tggtaattcg ccgatcaaat cctccttcaa acaataattt gaaaaagaaa
      721 accaacatca ccccaaatag caaaggctcg gtgggtaagg taagtgccgc atattcatta
      781 aactcaacgg ttaatgaaaa gggagtgaaa aacagaatga agtaaaccag cttatctagt
      841 gaaacaagcg ccgaaaaaag aatgagaagc actactggaa tcaacggcag cagataaaac
      901 tcctgcgtca aaagccagga atttagcgcg ataaaagcac taacaatccc ataaaaccaa
      961 gccgatgggc ggggcagcaa acgggctaat tgctcattca aaacgactgc ggattcgctg
     1021 aatattttcg atgatgatag taagaactac agcaagcaag aaagtcgcaa cggtcgatac
     1081 caatacaatt aaccaacgta ctggatagct tttcttttca gcaggtttgg cgagattcac
     1141 cacgaattta tgtgggattt tatccgtcgc atcaactttc gcttcctcgt atttggtttt
     1201 caaacgactc aacgattcgc ggtcgtgttc catgtcttgt gtaatagacg tgtacaaacc
     1261 tccgtattta gcaaggatgg ccattttctc atcaatgctt cgggttgctg atgcacttcc
     1321 tgccatcaat gctttaccat aagcctcact caaacgttca acctgagcat cgtaatcgag
     1381 tacacccaaa tcgcgcagct tgttcaatgt gtcttcgcga gcttgtaggt aatgtgtgaa
     1441 atcgaaatat tccttttgta caattgccaa agcttgtgaa gcacgttcgt gttgcatacg
     1501 ggttttaacc gtgtccaata aagtacaaat ctcattcgcg atatcggctg ctagcttagg
     1561 atcttcatcc attacatcca cacgtaccga taaatattcg gttcggttaa acgagatgtt
     1621 ttcttcaaac ttcttgttca actcggtcat gggatagcga ccatcggtat caatccgata
     1681 gtgctccatt aggttgtatt tcgagatcaa acgttcccga atctcatccg aattaagaat
     1741 ttgaagcatt tgctccactt gctcttcttc cccaaattgg aggatattct ccttctcaaa
     1801 agaattctca ctcaacaacg atttcgaaat cgaactgttg gtggtcggga acataatcac
     1861 cgatgatttg aaccgaggct tgatgaaaaa ttcagaagaa aaaacgactg aagcaatcag
     1921 agctgccaat gttgcgataa ggataggctt gcgtttcttc cagagaaata tcaacagatt
     1981 tgttgaatca aacaggcttc ccggttgtgt tgactccata agtggattaa attcttactt
     2041 aatgtttgcc cgcaaaagta gcattttcag cgcatttgcc ttacttaccc gtatttaaga
     2101 atccggaata tcgattttac atgcagtaaa ccaatggaaa aagcaaaagc tgccgaactt
     2161 ccagagaata caaccatgcc caatatccaa ttctgaaata gggtttgact tacccaagcc
     2221 aatatcacca cacacggaat aaaaatgctc aactgaacca ttaaacgtgt atttactttg
     2281 aatttgaaaa tccgttgggc aaggtaaatc tgcaataaag cggttacaaa ctgggtgata
     2341 agactagata aggcagcgcc ttgggcttta aaatgcggaa tgagaatgta gttcagagtt
     2401 aaattgaaaa ccattcccga agctgccata atgtttaact gaagcagatt tccattggct
     2461 gtcaacaaag tcccgaaaat gtaggatagg gaagaggcaa taaaacatcc cattaatatt
     2521 gaaaatactt gagccgattc aagtgcatgt tctttacaca tcaaaaccat caactcctca
     2581 ttataaaaaa tacatccgat ggtaatgacg attgccggtg tgagtaaaat cgtaaatgcc
     2641 agtttaacca attgctcaac cgattcttta taccgtagca tgcgagcaaa catgggcaat
     2701 agcaagccgg cgaataaaaa tgaaatcata ttagccgaat cgagcaaacg ataggcagat
     2761 gcataaatcc cactttcggt agctccttcg ggtaagatac gttctaacat cacagtatca
     2821 acccgattat aaaacgtcat caacaaaact aaaatggcaa aagggaaact cttttttaga
     2881 atgagaaaga agaatggacg attccatttt aaccgcttaa aagctgcttt tcgtacaact
     2941 atgaaaaagg taatcagggt ggttaataaa tacgaaaagg tttgggtgta tataaaccat
     3001 tcaatctgaa aaggcccttc tgtaacatgt ccccaaagca aaatgctgca aaacaaaatc
     3061 atcaaactcc tatcgagaac cgaaataata ctatcggttt taaatagatg cagtccggct
     3121 aaattcgaac gcatatacaa aatcatgaaa gctagaaact ggttgaagcc tagcatggct
     3181 aataaaccca attgttgagg tgaatagcca atcagaaatc ctgcagccaa ggtgaccacc
     3241 ataaatacaa tccctaacaa tagctttaag gctaaaatgc ttgagaagtg tttgttaatc
     3301 aggtggttgt tttgagcaat atttttattg ttgaaattgg taattccaaa atccaacaga
     3361 atattgagca gaaacgaaaa gttgaaaata gcgaagtaca tcccgtaggc ttctgtacct
     3421 actgtttgct gcacagcccg atcaatacca agtatccaaa atggcttgat aagcaaatta
     3481 agcgtgagca gtaaagccag attctggatg aacttttttt gcatggttgg gtgccgactt
     3541 aacgcaaacg atcagcagaa cgtactaaag catcgtcttt tctaattgca cgattggcta
     3601 ggaaaataaa taacactcca attaatggta atgctaatcc ccatccatag gttccttctg
     3661 aaactgtacc tgaaattttt tctagtgttt tatttatggt tgcaatgagc aataacaaca
     3721 tcgcagtatt cgcgagcatt aaagatgctg ttagacgtaa ttgtaacgct cttttcttaa
     3781 acagaaatag gataattaaa gggaaaatca tcaaaataat tcccactgca actaacgatg
     3841 gttggctttg aattacctct tcaccttgaa ttaatccacc taaataaacg gtgtatgatt
     3901 tatcctcaac agtagtattg taaagtggtg cggctagcaa aactcctgaa caaactgctg
     3961 caagaaataa atacaaactt tgaatgcgtt gaatcatatg ttactttcaa aaggttgcaa
     4021 aaatagcgaa gcttaagtct attccggaga taaaaatgat tcaggtggct tgcaaaaaca
     4081 aaatgttgta ttattgcaca atcatattac cctaattcat ttcggttttg catcatcttt
     4141 cagccctcaa tgctgaaaag cttccccgaa tatcccccac ataattaaga atccgagtca
     4201 ttttaatacg gtaaatccgc atgtacgaca ttctcgaact gaatgccaag cttgtaggtg
     4261 aattgcgtga aatcgcccta aagcttaaca tccccaaagc agaagccctt aaaaaacaag
     4321 atctggtata ccggattttg gatgaacagg cacttaaccc tactgaagcc agtcgattga
     4381 aaaagaaaga agaaagtgcc aaagcggcta ataagcctgc tgctgatgtc acacctaaga
     4441 aaaacgtcga gacggtaaga ataacgctcg atccggcaga agttaggaag cttgcttctc
     4501 aagcaattcg agacgaaaaa acacctgcag aaaaaactcc agtagctaaa aaagcagcgg
     4561 aagaaaaacc ggctccaaca gaagtaaaac cacttcgagg ccgccgtcca aacgcatcta
     4621 aagttcctcc tgccgtttta cccgaaaaag tagaagagcc agctgctcaa acaccttcgc
     4681 caactcctgc tccatcacct aaaccacacg ttcgtcaacc tcacccaatt aaacgaacag
     4741 atgtagcaat cccaaattcg gaattagctt cagaaaaacc taatacacct ccaccagccg
     4801 aaggagaagt agttcgcaac gttcctactc ctcatccaga tggtaaaata attcgtaacg
     4861 aacgtcgtga tcgtactaat agatcacttt tcgataaacg ttctgataa
//
LOCUS       DAOOQW010000183         4874 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_20791_length_4874_cov_6.173480, whole genome shotgun sequence.
ACCESSION   DAOOQW010000183 DAOOQW010000000
VERSION     DAOOQW010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4874
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20791_length_4874_cov_6.173480"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="PK281_09400"
     CDS             complement(<1..203)
                     /locus_tag="PK281_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020212405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49597.1"
                     /translation="MIIDLKVPSPGESISEVEIANWLKKEGDYVEKDEELCEIDSDKA
                     TLTINAESAGKLSIKVEAGTTVQV"
     gene            complement(313..>1998)
                     /locus_tag="PK281_09405"
     CDS             complement(313..>1998)
                     /locus_tag="PK281_09405"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008611782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HOY49598.1"
                     /translation="VQMAKLDGYKTGGTIHIVVNNQVGFTTNYLDGRSSIYCTDVAKV
                     TLSPVFHVNADDVESVVYAVQLAMEFRQQFKGDVFIDLLGYRKYGHNEGDEPRFTQPI
                     LYKAIAKHPNPRQIYFDRLVQEGVISKEEATELDSNLKKNLESALEDARKRERTVINP
                     FLGDQWKHVRPSKPSDFDTSPETGVALKQLQSIAKVLYTVPTGMTVFKKMEKLLNDRK
                     TMVEETGILDWGMAELLAYGTLLKEGNPIRFSGQDVERGTFSHRHAVLKIEDSEEEYI
                     PLTQLAKDQASFQIYNSLLSEYGVLGFDYGYSLAMPDTLTVWEAQFGDFNNGAQIIID
                     QFISSGEDKWRSMSGLVMLLPHGYEGQGAEHSSGRMERFLTLCAEQNMYVTNVTTPAN
                     FFHMIRRQLKSDIRKPLIVFTPKSLLRHPRCVSKMADLTKGKFQELIDDANASASKVE
                     KVVLCTGKIYYDLLEEKETRKLDSIALVRLEQLYPLPQKQLDAILKKYNKAKQIVWAQ
                     EEPENMGAWSYILRNLRHLNIQVASRAESGSPATGSSKRHAVEQKKLLKSVLG"
     assembly_gap    1999..2098
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2099..2913)
                     /locus_tag="PK281_09410"
     CDS             complement(<2099..2913)
                     /locus_tag="PK281_09410"
                     /inference="COORDINATES: protein motif:HMM:NF027404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HOY49599.1"
                     /translation="MDSFSYISNADPSALDHLYQQYLSNPSNVDESWGKFFEGVEFAG
                     KNYPTKPNSASSDAIPENVSKEFKVINLINGYRSRGHLFTRTNPVRERRQYTPTLDAV
                     NFGLSDADLDTVFQAGSLIGIGAAKLSVIISHLQETYCSSIGVEYMYMRNQEAVAWIQ
                     SRMESSRGHAKFSVEDKKQIFAKLNDAVGFESFLDKKFVGQKRFSLEGCEALIPALDQ
                     VIENGSKLGIREFVMGMAHRGRLNVLSNIFKKDARSIFTEFEGKEYDDDGEFSG"
     gene            complement(2988..3389)
                     /locus_tag="PK281_09415"
     CDS             complement(2988..3389)
                     /locus_tag="PK281_09415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49600.1"
                     /translation="MSSNEQRYPQLTDSASSIEPYFIQKDLLRELILQLKKDFASAGF
                     PIKLLLTQKYSFDELCTHLQNAFRDRSAQQLFNLLYRVDVSEKQLVQGMPTPGIDDRL
                     FAEIIVKRELQKVVLRKLYSSKSPQTTEEDF"
     gene            complement(3417..4316)
                     /locus_tag="PK281_09420"
     CDS             complement(3417..4316)
                     /locus_tag="PK281_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49601.1"
                     /translation="MEAKTEKKDNRLVWILAILLLISVVGNVYLLNRSTTAEKQVVEE
                     RAQVDSLNSFKVALENDFQAMTFELDQFKGKNIQLDSLLEKANGDIAKQKQKIDFLIK
                     ENKDIDLLKRQLAEMKAIRDGYRQQIDQLIKENKELRFANINLTQEVDNLNKTTQDLS
                     QKVELASVLKAESISVKTLREKSKGRMEETEKAKRVSRITTTFVVAENKVAPKGDREI
                     VLRIIKPDGYALTDPNNASGTFVKEDGRSSEFTLRKSIQYNNNREEVIFDWDQLEELK
                     SGLYVSEIYLDGKLMGNYKFNLK"
     gene            complement(4478..>4874)
                     /locus_tag="PK281_09425"
     CDS             complement(4478..>4874)
                     /locus_tag="PK281_09425"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOY49602.1"
                     /translation="LPDQQPQAIYRLPAQLIQAGILTALSYEGDMEAMGTRNLGFLAG
                     TASAYGLSNSEALNLISLNPAKILGVDAQDGSLKVGKKACFFISNGNALDMMYLTVNQ
                     IYIDGKIVDNSTFQKDLYQKYQKKYGIIK"
BASE COUNT         1338 a          956 c          951 g         1529 t
ORIGIN      
        1 acttgaactg ttgttccagc ttctacttta atgctcagct ttcctgccga ttctgcatta
       61 attgttagtg tggctttgtc ggaatcaatt tcacagagtt cttcatcctt ttctacataa
      121 tcgccttctt tttttaacca gttggcaatt tctacttcac ttattgattc tcctggagaa
      181 ggtactttca gatcgataat catgttgatt taattctttt tgttgggtgt caattttatg
      241 aattcgcctt gcggattttg tagactccaa ttgcaatctg gaaaaattag aacaacggag
      301 agaacaaatt gatcatccta aaaccgattt cagtagtttt ttctgttcaa cggcgtgtcg
      361 ttttgaactt ccagttgccg gacttccgct ttcggcgcgg gatgcgactt gtatattcaa
      421 atgacgaaga ttgcgaagga tatatgacca agctcccatg ttttcgggtt cttcttgagc
      481 ccatacgatt tgcttggctt tgttgtactt tttcaaaata gcatccaatt gtttttgagg
      541 taatggatac aactgttcca aacgaaccaa tgcaattgaa tcgagtttgc gtgtttcttt
      601 ttcctcgagt aaatcgtagt aaatttttcc tgtacaaagc accactttct caacttttga
      661 ggcagaagca tttgcatcat caattaactc ttgaaatttt ccttttgtta aatcagccat
      721 tttgctcacg cagcgcggat gacgaagcaa actcttaggt gtaaacacga tgagcggttt
      781 acgaatgtcg cttttcagct gacgacgaat catgtggaag aaattcgcag gagtagttac
      841 attcgttaca tacatgtttt gttcggcaca cagggtgaga aaacgttcca ttcgtccgga
      901 actatgttcg gcaccttgtc cttcgtaacc atgaggtaat aacatcacca atccactcat
      961 gcttctccat ttgtcctcac cagagctgat gaattgatcg atgataattt gagcgccatt
     1021 attgaaatcg ccaaattgtg cttcccaaac cgttagcgta tcgggcatag cgagtgaata
     1081 gccataatca aaaccaagaa ctccatactc gcttagcagt gaattgtaga tttgaaatga
     1141 agcttgatcc ttagctaatt gcgtaagcgg aatatattct tcctctgaat cttcaatttt
     1201 caataccgcg tgacgatgag agaatgttcc tcgttccaca tcttgacctg agaaacgtat
     1261 tggatttcct tctttcaata aggttccata ggctaacaat tcagccattc cccagtctaa
     1321 aatacctgtt tcctcaacca tcgttttacg gtcgttgagg agtttctcca tttttttaaa
     1381 gacggtcata cccgttggaa cggtatacaa aaccttagca atagattgaa gttgtttgag
     1441 cgcaactcca gtctctggtg aagtatcaaa atcggatggt ttagatggac gaacatgttt
     1501 ccattgatca cctaaaaatg gattaatcac ggtgcgttca cgtttacgcg catcttccaa
     1561 ggccgattct agatttttct tcagattaga atcaagttct gtggcttctt cttttgaaat
     1621 aacaccttct tgaaccaatc gatcaaaata aatctgacga ggattcggat gtttggctat
     1681 tgctttatag agtattggtt gggtgaaacg aggttcgtca ccttcgttgt gtccgtattt
     1741 tcggtatcca agcaagtcga taaatacatc gcctttaaat tgctggcgga attccatcgc
     1801 taattgaacg gcatatacca ctgattcaac atcatctgca ttcacgtgaa acacagggct
     1861 gagtgtaact ttagcaacat ccgtacaata gattgaagaa cgaccatcga ggtaattggt
     1921 agtaaatcca acttggttat ttaccacgat gtggatggtt ccacctgttt tgtagccatc
     1981 gagtttggcc atctgaacnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncc
     2101 tgagaattcg ccatcatcat cgtattcttt cccttcgaat tcggtgaaaa tactgcgcgc
     2161 gtctttcttg aaaatatttg aaagaacatt caatcggcca cggtgtgcca ttcccataac
     2221 aaactcacga attccaagct tagaaccgtt ttcaattacc tgatcgagtg ctggaataag
     2281 ggcttcgcat ccctcgagcg agaagcgttt ttgtccgaca aattttttgt ctagaaaact
     2341 ctcaaaacca acggcatcgt tcagtttagc gaatatttgt tttttatcct ccacgctgaa
     2401 tttagcgtga ccacggctac tttccatgcg agattgaatc caagcaacag cttcttgatt
     2461 gcgcatgtac atgtattcaa cacctatact tgagcaatag gtttcctgca aatggctgat
     2521 gatgacgctt agtttagccg caccaattcc aatgagcgat cctgcttgaa aaacggtatc
     2581 aagatcagcg tcgcttaatc caaaattgac agcatcgagt gtaggcgtgt attgacggcg
     2641 ttcgcgaaca gggttagttc gtgtaaaaag atgtccgcgg gagcgataac cattaattag
     2701 gttaatgact ttaaattctt tgctaacgtt ttcgggaatt gcatccgaag aggcagaatt
     2761 gggttttgtt ggatagttct tgccggcaaa ttcaactcct tcgaaaaact tcccccagct
     2821 ttcatcgaca ttagaagggt tacttaggta ttgttgatac aggtgatcca aagcagaagg
     2881 atctgcattg gagatatagg aaaatgagtc catcgggatg ggtaaaattg gagtgcaaat
     2941 ttaagagaaa atgcaaggcc aatcgggaaa gaagcaagac agattgatta aaaatcttct
     3001 tcggtagttt gagggctttt gctgctatat agttttcgga ggactacttt ctgtaattca
     3061 cgtttgacaa tgatttcggc gaataaacga tcgtcaattc cgggagttgg catgccttga
     3121 acgagttgtt tttcggacac atctactcgg taaagcagat taaataattg ttgggcactg
     3181 cgatctcgaa aggcattttg taggtgcgta cagagttcgt cgaaagaata tttctgcgtt
     3241 aagagcagtt taatgggaaa ccctgcggaa gcaaaatctt ttttaagttg aagaataagt
     3301 tcccttaaaa ggtctttctg aatgaaatat ggttcaatac tagaagccga atctgtaagc
     3361 tgtgggtatc gttgctcgtt tgaactcatg caggaaagga tacccgaatg aacggtttat
     3421 ttcaaattga atttgtaatt ccccatgagt ttaccatcga gataaatctc agaaacatac
     3481 aagcctgatt taagttcttc caattgatcc caatcgaata taacttcctc acggttatta
     3541 ttgtactgaa tggatttacg taatgtaaac tccgaagaac gaccatcttc tttcacaaat
     3601 gtaccgctgg cgttattcgg atcagtaaga gcgtaaccat ctggcttaat gatgcgaagt
     3661 actatttcgc ggtcgccttt gggtgcaact ttattttctg caacaacgaa agttgtagta
     3721 attcgactca cccgtttagc cttttctgtt tcttccattc gacccttact tttttcgcgc
     3781 agggttttta cagatatact ttcggctttt aaaacagatg ctaattcaac tttctgactt
     3841 aaatcctgag ttgttttatt caagttatcg acctcttgag taaggttgat attggcaaaa
     3901 cggagttctt tattttcttt gattaattga tcaatttgct gacgataacc atcgcgaatg
     3961 gccttcattt cagctagttg gcgttttagt aaatcgatgt ctttattctc tttaattaag
     4021 aaatcgatct tttgtttttg cttagcaata tccccatttg ccttttccag taggctatcg
     4081 agttgaatat tttttccttt gaattgatcg agttcaaacg tcattgcctg gaaatcgttt
     4141 tccaaagcaa ctttaaatga attaagagaa tcaacctgag cacgttcttc aacaacttgt
     4201 ttttcggccg tggtagaacg gtttaacaaa tacacattgc caacaacaga aattaaaagc
     4261 aggatcgcaa ggatccacac taagcggttg tctttttttt ctgtctttgc ttccatagta
     4321 ttactaagag atgcgtttgt gaaagaatct cagcgctgca aaaataacaa tttttaaagg
     4381 ggggagactc gtttgaaggg ttaaaaatca gtgaaactta cacaagccta actggatgaa
     4441 tttcatgcaa atacgaatct tacatgcaag aaagaaatca cttaattata ccgtattttt
     4501 tctggtattt ctggtataaa tctttttgaa aggtagaatt atcaacgatt tttccatcaa
     4561 tataaatttg attcacagtc aaatacatca tgtctaaagc attcccgttc gagataaaaa
     4621 aacaagcttt cttacccact ttcagacttc catcttgagc atcaacgcct aatattttag
     4681 ccggattaag ggaaattaaa ttgagagctt ccgagttgct caaaccataa gctgaagcag
     4741 ttccagctaa gaagcccaaa tttcgggttc ccatggcttc catatcgcct tcataggaaa
     4801 gggctgtaag aattccagct tgaattaatt gagctgggag acgatatatg gcttgaggtt
     4861 gttgatccgg caag
//
LOCUS       DAOOQW010000184         4847 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_20931_length_4847_cov_4.733306, whole genome shotgun sequence.
ACCESSION   DAOOQW010000184 DAOOQW010000000
VERSION     DAOOQW010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4847
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20931_length_4847_cov_4.733306"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            175..477
                     /gene="trxA"
                     /locus_tag="PK281_09430"
     CDS             175..477
                     /gene="trxA"
                     /locus_tag="PK281_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008240910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOY49603.1"
                     /translation="MATFQELINDETPLLIDFSAEWCGPCKMLAPILKEVAGQLGDQV
                     RIIKVDVDKNPQAAGAYQIQGVPTLILFKAGKQLWRQSGVVPANQLMQIIRQQIAQ"
     gene            complement(601..1641)
                     /locus_tag="PK281_09435"
     CDS             complement(601..1641)
                     /locus_tag="PK281_09435"
                     /inference="COORDINATES: protein motif:HMM:NF016159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HOY49604.1"
                     /translation="MIVYSSASLHQTAVHFVGNPSNDDPLTLSESLLEIQDDDQEPIL
                     NYLMRAFKPEAFYHFTTNGNLVYDAVKELFDENGNFLEASQTIASHLYDVSTHPRILA
                     GDLFVARIREVSLHNEYADCIVLLKAEVEDKFAQVEWADKHSQVKLSNGYRLDRIDKA
                     CLIFSLEPGDGYRVLVVDKTNGNSAEAQYWTDAFLELETLEDSYHFTQQYMQLTKEFV
                     KEQLPENFEVNPAEQIALLNRSSKFFKEKQQFDFDNFADEVMQQPEVVSAFRKFTESY
                     SEDPDHSISENFDISQAAVKKHAGMFKSVLKLDKNFHIYVHGNRQLIERGYDDEKGMN
                     YYKVFFSNEQTT"
     gene            1703..2569
                     /locus_tag="PK281_09440"
     CDS             1703..2569
                     /locus_tag="PK281_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family ion channel"
                     /protein_id="HOY49605.1"
                     /translation="MIRYNPKDWFTFIFRFHKADTFRTLAPLIFLIGLYAGVIAWLEI
                     EVWQVGEKSHIRNLSLIHSTLGLVISLLLVFRTNTAYERWWEGRKVWGSLVNSSRNLA
                     MKLSVLATDDRAFFRAMIPNYAFAMKNHLRGVFLHKELVDCDDFVSSELVENQHVPNQ
                     IAQMMFRRLAEMEQQGKLKVEHLLLINTEMVNFTDAVGACERIKKTPIPLSYSVFLKK
                     FIFLYVMTLPFGYVFSLGYWVMPVVAIIFYVLASLELIAEEIEDPFGKDANDLPTDEI
                     AKTIRQSVEDLL"
     gene            2630..3058
                     /locus_tag="PK281_09445"
     CDS             2630..3058
                     /locus_tag="PK281_09445"
                     /inference="COORDINATES: protein motif:HMM:TIGR02293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2384 domain-containing protein"
                     /protein_id="HOY49606.1"
                     /translation="MKTYPINETPISYTVAESAAAYGLINFIRGGLPFDFFVGLGNRL
                     NFSFREWARVLHLSERSLQRYQKGQKAFDAMQSERIIEITMLSNYGKDIFGSDERWVE
                     WLSMPNLALGGITPQSLLDSSMGIQLIKDELGRLEYGIVS"
     gene            3055..3510
                     /locus_tag="PK281_09450"
     CDS             3055..3510
                     /locus_tag="PK281_09450"
                     /inference="COORDINATES: protein motif:HMM:NF020387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HOY49607.1"
                     /translation="MIVFRLTRREFSLDLSGIGAEKLGGRWNSRGNPVLYTASNRALC
                     LTEWLVHTPSVWLPTDLVMVQIEIPDSASIHQFQDLPKDWYQFPHPLSSRKLGDEWLL
                     KSETLVLSVPSAVVPEEFNYVINPKHPDMKEVRILSSVDFRLDERFMTA"
     gene            complement(3535..3984)
                     /locus_tag="PK281_09455"
     CDS             complement(3535..3984)
                     /locus_tag="PK281_09455"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HOY49608.1"
                     /translation="MIHTSNELFEYFKSHIGKSYPKDMNTFGAWLQPIIKKVEREELH
                     LEFALRPEEGNPAGTVHGGVIAAIIDETIGAMMYYTGEPHFKSTINLSVDYLNSCRPG
                     DVILCRARLVKAGKTLLHATADILREDDGKIIASGSSSLIVMVKSAS"
     gene            complement(3981..>4847)
                     /locus_tag="PK281_09460"
     CDS             complement(3981..>4847)
                     /locus_tag="PK281_09460"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007840073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HOY49609.1"
                     /translation="PRFNPYFIPKMLVNMPSGVVSIRTGFRGIVYTPVSACATGNTAI
                     MDAANYIRLGKADVMIAGACEAPITYSAIGGFGAMKALSTRNDDPQKASRPFDKDRDG
                     FVMGEGAGALILESLSHALARGANILGEVAGTAMTADAYHLSSGLPDGDGARRCMLLA
                     LEEAGLSPDQVDYINVHATSTPVGDPGEMKAVWSAFGDRSSLLISSTKSMTGHLLGAA
                     GAAEAIISLGAIQRQAAPPTINLENRDPEIPAGLNIVSGVAKAHPIRVVLSNTFGFGG
                     HNSSVILKAYSA"
BASE COUNT         1336 a          935 c         1135 g         1441 t
ORIGIN      
        1 acgatatacg atatacgata tacgatatac gatatacgat atacgatata cgataatgaa
       61 tcttagatgc tgagaaagag ggaaaatcgg gcttgttgta catctgttta gcgtgacaga
      121 tgtcatggac tagggagatt gctagggcgt atttttgtgg tagatattaa aacgatggca
      181 acatttcagg aattaataaa cgacgagacg cctttactga ttgatttttc ggcggaatgg
      241 tgtgggccgt gtaaaatgtt ggcgcctatt ttaaaagagg tagctggaca actgggcgat
      301 caagtgcgca ttattaaagt ggatgtagat aaaaatcccc aagcggcagg agcttatcaa
      361 atacaaggtg ttccaacctt aattctgttt aaagcgggaa agcaattgtg gcggcaaagc
      421 ggagttgttc cggcgaatca attgatgcag attattcgtc agcagattgc tcaataggaa
      481 cgcttcttta tagcataaac gctcgcggca tgccgtcgct aaagctatgg catgcaacgc
      541 gattaatagc ttgggagcgc cctaaaaaga aaagcccccg ttgaggcgga ggcttttggt
      601 ttaggtggtt tgttcgttgc tgaaaaacac cttgtagtaa ttcattcctt tttcgtcgtc
      661 gtagccgcgt tcgatgagtt ggcggtttcc gtggacgtag atgtggaagt ttttatcgag
      721 tttgaggacg ctcttgaaca tacccgcgtg cttttttacg gctgcttgag aaatgtcgaa
      781 attctcgcta atgctgtggt cgggatcttc tgaatacgat tcggtgaatt tgcggaatgc
      841 actaaccact tcgggctgct gcatgacttc atcagcaaaa ttgtcgaaat cgaactgctg
      901 cttttccttg aagaacttag agctgcggtt caataatgca atttgttcgg caggattcac
      961 ttcgaaattt tcgggaagtt gttctttcac aaattccttc gtgagctgca tgtattgctg
     1021 ggtaaaatgg tagctgtctt cgagtgtttc tagttcgaga aatgcatcgg tccagtattg
     1081 tgcttctgca gaattaccat tggttttatc aactactaac acacgatagc catcacccgg
     1141 ctctaaactg aaaatgaggc aggctttgtc gatacgatcg aggcgatatc cattagaaag
     1201 cttcacctga ctgtgtttat cagcccattc tacttgtgca aatttatctt ctacctcagc
     1261 tttgaggagc acgatgcaat cagcatattc gttatgcaga cttacttcgc ggattcgtgc
     1321 tacaaataaa tcaccagcta aaatgcgtgg atgagttgac acgtcatata agtgactagc
     1381 aatggtttga gacgcttcga ggaaatttcc gttttcgtcg aaaagttctt ttactgcatc
     1441 gtaaacgagg ttgccgtttg tcgtaaaatg gtagaacgct tccggcttga aagcgcgcat
     1501 gaggtagtta agaatcggtt cctgatcatc gtcctgaatt tcgaggagac tttcggaaag
     1561 tgtgagcgga tcatcgttag aagggtttcc aacaaagtga acagcggttt gatgcagcga
     1621 agcggacgaa taaacgatca tgcggcgaag gtaatgaaag tgtattcgat agttgcaatc
     1681 tgcttcgtag gtttgcaaaa ctatgattcg atacaatccc aaagactggt ttaccttcat
     1741 ttttcgcttt cacaaagccg atacgttccg cacattagct ccactcattt tccttattgg
     1801 cttatatgct ggagtgatag cttggctcga aattgaagtt tggcaagtgg gtgaaaaaag
     1861 ccacattaga aacttatcgc tcattcatag taccttagga ttagtgattt cgttattgtt
     1921 ggtttttcga acgaacacag cctatgagcg ctggtgggaa gggcgaaaag tttggggcag
     1981 tttggtgaat tcgagccgga atttggcgat gaaactatct gtacttgcta ccgatgatcg
     2041 agcttttttt agagccatga ttcctaacta cgcatttgcc atgaagaatc atttacgcgg
     2101 tgtttttttg cataaggaat tggtggattg tgatgatttt gtttcttccg aattggttga
     2161 aaatcaacat gtaccgaatc aaattgctca gatgatgttt cgtcgattgg ctgagatgga
     2221 acaacagggt aaattgaaag ttgagcattt actgttgatc aatactgaaa tggtcaattt
     2281 tacagatgca gtaggcgctt gcgagcggat taagaaaaca ccaattccct tatcatacag
     2341 tgtgttttta aagaaattca tttttctgta tgtgatgaca ttaccttttg gatatgtttt
     2401 ttcgttgggt tattgggtga tgcctgtggt tgcgattatc ttttacgtgt tagcgagttt
     2461 ggaattaatt gcagaggaaa ttgaagaccc ttttgggaaa gacgcgaacg atttacctac
     2521 tgatgaaatc gccaaaacga ttcgacaatc ggtggaagat ttgttgtgaa aatatgtttg
     2581 gcatttgtcg ttttgaaaaa gacatgtatc tttggggaaa ggtttattta tgaaaacata
     2641 tcctataaac gaaacaccta tttcttatac agtagctgaa agtgctgcgg catatgggct
     2701 tattaatttt attcgaggcg gtttaccatt cgattttttt gttggcttag gcaatcgcct
     2761 gaactttagt tttcgagaat gggcaagagt gctacattta tctgaacgta gtttgcaacg
     2821 ttatcagaaa ggccagaaag catttgatgc catgcaatcg gaacgcatta tcgaaattac
     2881 catgttgagt aattatggta aagatatatt tggttcggat gaacgttggg tagaatggct
     2941 ttctatgcct aatctcgctt tgggaggaat tacaccgcaa tccttattag atagcagcat
     3001 gggaattcaa ctcattaaag acgagttagg tcgcttagaa tacggtatcg tttcatgatc
     3061 gtatttcgat taacacgacg cgaattctct cttgatttga gtgggatagg agcggagaag
     3121 ctaggcggaa gatggaacag tagagggaat ccagttctct atactgcgtc gaatcgggca
     3181 ttgtgtttaa ccgaatggct tgtgcatacg ccatctgttt ggttacctac cgatttagtc
     3241 atggtgcaaa ttgaaatccc tgattcagcc tcgatccatc aatttcagga cttaccgaag
     3301 gattggtatc aatttccgca tcctttatcc tcacgaaaat tgggtgatga atggttattg
     3361 aaatcagaaa cattggtctt gtctgtacca tcggcagttg ttccggaaga attcaattac
     3421 gtgataaacc cgaaacatcc tgatatgaaa gaagttcgta ttctttctag tgtggatttt
     3481 agattggatg aacgatttat gacagcttaa ccaataaagt atcagtctat aaaatcaaga
     3541 agccgatttc accattacaa ttaaactcga acttccggaa gcaataattt tcccatcatc
     3601 ttcccgaaga atatcagcag tagcgtggag taatgttttc ccagctttga ctaatcgtgc
     3661 acgacacaaa atcacatcac ctggtcgaca tgaattcaag taatcgacac ttaggttgat
     3721 ggttgatttg aaatgtggtt cacccgtgta atacatcatg gctccaatgg tttcatcaat
     3781 gatggcagct ataacgccac catgaaccgt tccggcagga tttccttctt cggggcgtaa
     3841 ggcaaattcg aggtgtaatt cttcccgttc gactttttta ataatcggtt gaagccatgc
     3901 tccaaaggtg ttcatgtctt tgggatagct ttttccgata tgacttttga agtactcgaa
     3961 cagttcgttg gatgtatgaa tcatgcggaa taagctttaa gtataacact ggaattatgt
     4021 ccgccaaaac cgaacgtatt gcttagaaca actcgaattg gatgtgcttt cgctactcct
     4081 gacacgatat tcaatcccgc tggaatctcc ggatcacggt tttcgaggtt gatggttgga
     4141 ggtgcggctt gacgttgaat cgcacccaaa ctgataatag cttcggcagc accggcggca
     4201 cctaggagat gtccggtcat agatttagtg gaactaatta gcaaactact tcggtctcca
     4261 aaagcactcc aaaccgcttt catttcacct ggatcgccca ctggagttga ggttgcgtga
     4321 acattgatat aatctacttg gtcaggagat aatccagctt cttcaagggc tagtagcata
     4381 catcgacgtg cgccatcacc atccggaagt ccagaactta aatgataggc atcggcagtc
     4441 atggcagtac ctgcaacttc acccaagata ttcgctccgc gagcaagtgc gtgagaaaga
     4501 gattccagaa taagcgctcc ggcaccttca cccattacaa atccgtcgcg atctttatcg
     4561 aagggacgag aagctttttg aggatcatcg tttcgggtgg aaagtgcttt catcgcgcca
     4621 aaaccaccga tagctgaata ggttattggt gcttcgcatg ctccagcaat cattacatcg
     4681 gctttcccca aacgaatgta atttgcagca tccatgatag ctgtattccc agttgcacaa
     4741 gccgatacag gcgtataaac aattccccgg aaaccagtac gaattgaaac aacacccgaa
     4801 ggcatattta cgagcatttt gggaatgaaa tacggattga atcgagg
//
LOCUS       DAOOQW010000185         4806 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_21139_length_4806_cov_4.868238, whole genome shotgun sequence.
ACCESSION   DAOOQW010000185 DAOOQW010000000
VERSION     DAOOQW010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4806
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21139_length_4806_cov_4.868238"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /locus_tag="PK281_09465"
     CDS             <1..613
                     /locus_tag="PK281_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HOY49610.1"
                     /translation="IGSSPEAQLQVSNSKAHIYPIAGTFRRSGNDQEDAALAEKLAAD
                     EKENAEHIMLVDLARNDMSRNADHVHVEVFKEIQFYSHVIHLVSKVTGNISKDASVVR
                     MAADTFPAGTLSGAPKHMAMTLINKYENVNRSFYGGAIGFIGFDGSFNHAIMIRTFLS
                     HENKLICQAGAGVVSKSSAESELQEVNNKLTALRKAVSMAEKI"
     gene            705..1271
                     /locus_tag="PK281_09470"
     CDS             705..1271
                     /locus_tag="PK281_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014781411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HOY49611.1"
                     /translation="MKILVLDNYDSFTYNLVHIIRALDYPLDIYRNDKITIEDVKKYD
                     KILLSPGPGIPDEAGIMKQLVKEYGATKSILGVCLGHQGIGEVYGAELYNIPKVLHGV
                     TSIATVSDSSDYLFKGVTPTFQATHYHSWAVRPESFTDDLRITATNDEGLVMALSHVK
                     FDVKGVQFHPESIMTPEGPKMIANWLKN"
     gene            1311..2300
                     /gene="trpD"
                     /locus_tag="PK281_09475"
     CDS             1311..2300
                     /gene="trpD"
                     /locus_tag="PK281_09475"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010179583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HOY49612.1"
                     /translation="MKKILAELIDHRSLTKDTAKQVLVDLATGKFNPSQTTAFMTVYM
                     MRSITVEELQGFRDAMLELCIPARFDQPVMDVCGTGGDGKNTFNMSTLSSFVVAGAGQ
                     PVAKHGNYGVSSACGSSNLLEHFGYKFTNNVDELKRSLERANICFMHAPLFHPAMKNV
                     APIRKELGVKTFFNMLGPMVNPSFPTRQLVGVFSLELARQYGYLYQNTDKDFVILHSI
                     DGYDEVSLTGSFKYFFNGGEKMAEPADLNLPQLKYDDIKGGNAVEESANIFMNVLNGK
                     GTPEQNAAIVANAGMALFCGHQKEGIPKAMERASESLKSGKALNAFKKLMENS"
     gene            2425..3231
                     /gene="trpC"
                     /locus_tag="PK281_09480"
     CDS             2425..3231
                     /gene="trpC"
                     /locus_tag="PK281_09480"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013070334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HOY49613.1"
                     /translation="MNILDQIIEHKHKEVAERKSLYPVKLLEQSIYFATQPVSMTKYI
                     QRDDKTGIIAEFKRKSPSKGIINAHASVERTSIGYMQAGASALSVLTDKQFFGGSNED
                     LMVARKFNFCPIIRKDFTIDEYQIIEAKSIGADAILLIAAVLDPKQSKEFTTLAHSFG
                     LEVLLEVHNEEELNQNLEVGANMIGVNNRNLKTFEVSIENSKRLAPLIPKDVVKVSES
                     GISSPEAILELKKFGYEGFLMGENFMKHSRPEIAAREFTELLRQQLIVNS"
     gene            3263..3898
                     /locus_tag="PK281_09485"
     CDS             3263..3898
                     /locus_tag="PK281_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006989757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HOY49614.1"
                     /translation="MKVKLKICGMRDANNIMEVASLKPDYFGFIFYPKSPRFVADDFI
                     LPVKLSPDILRVGVFVNETSSAIIEKAKTYPLDYIQLHGSETPEQCQALRQLGLGIIK
                     VFSVGDDFDFEITKPYKSVADYFLFDTKGKHHGGNASTFNWDLLKQYDQDIPFFLSGG
                     LSPENMNEVAKLDGMNIHALDVNSGVEASPGVKDISKLKQLINHIELIAKD"
     gene            3924..>4806
                     /gene="trpB"
                     /locus_tag="PK281_09490"
     CDS             3924..>4806
                     /gene="trpB"
                     /locus_tag="PK281_09490"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010228753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HOY49615.1"
                     /translation="MKYTVDERGYYGQFGGAYIPEMLYPNVEELRENYLRIIQQEDFQ
                     AEFHDLLKNYVGRPTPLYHAKRMSEKYGATIYLKREDLCHTGAHKVNNTIGQILVAKR
                     LGKKKIIAETGAGQHGVATATVCALMGMECTVYMGKIDMERQKPNVERMRILGTTVVP
                     ALSGSMTLKDATNEAMRHWINNPTDTHYIIGSVVGPHPYPDMVARFQSVISDEIKKQL
                     QEEIGNPFPDYVFACVGGGSNAAGAFYHYLNDEHVNLIGVEAAGKGVDSGESAATTAL
                     GKPGVLHGSKTLLMQTED"
BASE COUNT         1486 a          877 c         1151 g         1292 t
ORIGIN      
        1 gatcggctca tcacccgaag cacaattgca ggtaagcaat agcaaagcgc acatctatcc
       61 gattgcggga accttcagaa gatcaggtaa tgatcaggaa gatgcggcac ttgcagagaa
      121 attggcagca gatgaaaaag aaaatgctga gcacattatg ttggttgatc ttgcccgaaa
      181 tgatatgagc cgcaatgctg atcatgttca cgtggaagtc ttcaaagaaa ttcaatttta
      241 ttcgcacgtc attcatttgg tttcaaaagt taccggcaac attagcaaag atgcatcggt
      301 tgtgcgcatg gctgcggata cctttccggc cggaacatta tccggtgcgc ccaaacacat
      361 ggccatgact ttaattaata agtacgaaaa tgtgaaccgt agtttttatg gtggagcaat
      421 cgggttcatt ggttttgatg gatcgttcaa tcatgccatt atgattcgaa cgttcttaag
      481 tcatgaaaat aagttgattt gtcaggcggg agcaggtgtg gtttcgaaat caagtgcaga
      541 aagtgaattg caggaagtga ataataaact aacggcattg cgtaaggcgg tgtcgatggc
      601 agaaaaaata taagcggtta ccggttttca gttaccagtc attggtgact ggtaaccggc
      661 aactggtaac taaagaaaaa gtataaatct tcgaattaag aaaaatgaaa atcctcgtcc
      721 tcgataacta cgattcgttt acctacaatc ttgtacatat tattcgtgcg ctagattatc
      781 cgcttgatat ttatcgaaat gataaaatca caatagaaga tgtgaagaag tatgataaaa
      841 ttcttctttc gcctggtccg ggcattccgg atgaagcagg aattatgaag cagctggtga
      901 aagaatatgg agccactaaa agtattttgg gtgtgtgtct gggtcatcag ggaattggtg
      961 aagtgtatgg tgccgagctg tataacattc cgaaggtatt gcacggagtg acatccattg
     1021 caaccgtatc cgattcttcg gattatttat tcaagggcgt aacacccact tttcaggcaa
     1081 cgcattacca ttcatgggct gtgcgtcctg aaagttttac ggatgattta cgaatcacgg
     1141 ctacaaatga tgaaggactc gtaatggcgt tgagtcatgt gaagtttgat gtgaagggtg
     1201 tgcagtttca tcctgaatcc atcatgacac cagaaggacc caagatgatt gccaactggt
     1261 tgaagaatta aaacgatgta ttccgaataa ctaacaacat caacgattct atgaaaaaga
     1321 tattagctga attaatcgac catcggtcat taacaaaaga cactgccaag caagtgctgg
     1381 tggatctggc aaccggaaaa ttcaatccga gtcagacaac agctttcatg acagtataca
     1441 tgatgcgcag cattacggta gaagagttgc aaggctttcg cgatgcaatg ttagaacttt
     1501 gtatacctgc acgtttcgat caacctgtta tggatgtatg cggaacaggg ggcgatggaa
     1561 agaatacgtt taacatgtca acgctttcat cctttgtggt ggccggtgct ggacaacctg
     1621 tggcgaagca cggaaactac ggtgtatcat cagcatgtgg atcctccaat ttgctggagc
     1681 attttggata taagttcaca aacaatgttg acgaattgaa gcggagctta gagcgggcaa
     1741 acatttgttt tatgcacgca cctttgtttc acccggctat gaagaatgta gcacccatcc
     1801 gaaaagagct tggcgtaaaa acatttttca acatgcttgg cccaatggtg aatccttctt
     1861 ttcctacacg gcaacttgtt ggagtgttca gcctggagct tgcgcgtcag tatggatacc
     1921 tctatcaaaa taccgataag gactttgtaa tccttcattc tatagacggg tatgatgagg
     1981 tatcgctaac cggatcgttt aaatattttt tcaacggtgg tgagaagatg gcagaacctg
     2041 ctgatcttaa tttacctcaa ctcaagtatg acgatatcaa aggtggaaat gccgtagaag
     2101 aatcagccaa catttttatg aatgtattga atggcaaagg aacaccagaa cagaatgcag
     2161 caatagtagc gaacgcaggc atggcacttt tttgtggcca ccaaaaggaa ggtatcccaa
     2221 aagcaatgga gcgcgcaagt gaatccttga aatcggggaa ggcgttgaat gcattcaaaa
     2281 agttaatgga gaatagttaa tcgttaatag tgggtgtggg gtgtgtgcac gattagcttc
     2341 cttggtaaag aatatttaaa acgaacaaca aaatgacaaa gctggagact attaaccatt
     2401 aacgattaac ttttaactga ctacatgaac atactcgatc aaataattga acataagcac
     2461 aaggaggtag ccgagcgaaa aagtctgtat ccggtgaagt tgctggagca aagcatttac
     2521 ttcgctacgc aaccggtgtc gatgacaaaa tacattcagc gtgacgacaa gactggaatc
     2581 attgccgagt ttaagcgcaa atcgccaagc aagggaatta tcaacgcgca tgcatccgta
     2641 gaacgtacgt ccattggata tatgcaggcg ggtgcatccg cactttctgt tctaaccgac
     2701 aaacaatttt tcgggggaag caatgaagat ttaatggtgg caaggaagtt taacttttgt
     2761 cccatcattc gaaaagactt caccattgat gagtatcaaa tcatagaagc aaagtcgatt
     2821 ggtgcggatg cgatcttgtt gattgctgct gtgcttgacc caaagcaatc caaggagttt
     2881 acaacactgg cgcattcctt tggattagag gtattgcttg aagttcataa tgaagaagaa
     2941 ctaaatcaga atctggaagt cggtgcgaac atgattgggg taaacaaccg gaacctcaaa
     3001 acttttgaag taagtattga gaattcaaaa agacttgcac cccttattcc gaaagacgtg
     3061 gtgaaggttt ctgaaagtgg gatctcatcg ccagaggcaa ttcttgaatt gaagaagttt
     3121 ggttacgaag gttttttgat gggagagaat tttatgaaac acagcaggcc ggagattgcc
     3181 gcgcgcgagt ttacagagtt gctgaggcag cagttaatag ttaatagtta atagttaatg
     3241 gttaatagtt aacaggtttg atatgaaagt aaaactgaag atatgtggaa tgagggatgc
     3301 caataacatc atggaggtgg cttctttgaa acccgattac tttggtttta ttttttatcc
     3361 gaagtcgcca cggtttgtgg cagatgactt tatactaccc gttaaacttt caccagacat
     3421 tctgcgtgtt ggtgtgtttg taaacgaaac atcgtcagct attattgaga aagccaaaac
     3481 gtatccattg gattacatcc agttgcatgg aagtgaaacg cctgaacaat gtcaagcact
     3541 tcgccaactc gggcttggaa tcataaaggt attttctgtg ggagatgatt ttgatttcga
     3601 gattaccaaa ccatacaaga gtgttgccga ttattttttg tttgatacaa agggtaaaca
     3661 ccatggaggc aatgcttcaa cctttaattg ggatttgcta aaacagtacg atcaggatat
     3721 acctttcttt cttagtggag gattgtcgcc agagaatatg aatgaggtag ctaaattgga
     3781 tggcatgaat attcatgcgc ttgatgtgaa cagtggagtg gaggcaagtc cgggagtaaa
     3841 ggatatttcg aaattaaagc agctaattaa tcatattgaa cttattgcta aagactaatt
     3901 actaaagact atatactcat cttatgaaat acacagttga tgaacgcgga tactacggac
     3961 aatttggtgg agcctacatt cctgaaatgc tgtatccaaa tgtggaagaa cttcgtgaaa
     4021 attatcttag gataattcag caagaagatt ttcaagcaga gtttcacgac ttacttaaaa
     4081 attatgttgg ccgcccgaca cctttgtatc atgccaaacg gatgtcagaa aagtatggtg
     4141 ctaccattta ccttaagcgc gaagaccttt gtcataccgg tgcgcacaaa gtcaataata
     4201 ccattgggca gattctggta gcaaagcgtt taggtaaaaa gaaaattata gcagagaccg
     4261 gtgccggtca acatggtgtg gcaacggcaa ccgtttgtgc attgatgggg atggagtgta
     4321 ccgtctacat gggcaagatt gatatggagc gccaaaaacc aaatgttgaa cgcatgcgca
     4381 ttctgggtac tacggttgtt ccggcattga gtggaagtat gacgttgaaa gatgcaacga
     4441 acgaagcgat gcggcactgg atcaacaatc ctactgacac acattatatt atagggtcag
     4501 tcgttggtcc acatccgtat ccggatatgg tagcacgatt tcaatccgtg atcagtgatg
     4561 aaattaaaaa gcagttacaa gaagagatcg gtaatccatt tccggattat gtttttgctt
     4621 gtgtgggagg aggaagcaat gcagctggag ctttctatca ctacctgaat gatgaacacg
     4681 ttaatctgat tggtgtagag gcagctggca aaggcgttga ttctggagaa tccgcagcaa
     4741 ccactgcact tggaaagcca ggagttcttc acggaagtaa aactttacta atgcaaactg
     4801 aagacg
//
LOCUS       DAOOQW010000186         4781 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_21280_length_4781_cov_4.511003, whole genome shotgun sequence.
ACCESSION   DAOOQW010000186 DAOOQW010000000
VERSION     DAOOQW010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4781
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21280_length_4781_cov_4.511003"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1704)
                     /locus_tag="PK281_09495"
     CDS             complement(<1..1704)
                     /locus_tag="PK281_09495"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOY49616.1"
                     /translation="MASIQAQDCDSRLQLSISNPSTQAVSAQLFFKGTERIVNLKTST
                     SINTLCPGNYRLVILQSGYQPVDTSFVLTQSLDLHLQLIPEIKQLHEVEIHDDRSDEN
                     GFRTLQSSTLQAAAFQLNAGKPLGEMLKEVSGVSSLQSGPTISKPIIHGLHSNRILLI
                     NNGIRLESQQWGTEHAPEIDPFNVAKVTVLKGAAGLRFGSDAIAGVVLMEPYEMSSEK
                     GLKADVRLVGNSNGRQGGASARIEKTFGGKLQGMGLRIQGTLKNGGNVQTPHYYLANT
                     GMRERDASVSWQLNRKKLALELSGSVFSTQVGIFTGSHVGNVDDLLAAFSSSKPIDSV
                     GFERKIDRGYQTVLHGTGKARVCYHFSDTKKIELIYGLQHNRRKEYDSGLPYSNDPAV
                     MLAAQADFKISTHTADLLYQANLGKHYTLNSGLSLMTQGNVFQGLEYRALIPNFRNYG
                     SGVFAALTRQWKKWQAEAGLRYDYRWLECYRLDYSTLATYAVQRDFSNVSFSSGATYD
                     WTKQFSLSFHAGSAWRAPNVNELYGNGVHQSAASFEIGDSTLKSERAYQLSISGDSET
                     EK"
     gene            complement(1819..2208)
                     /locus_tag="PK281_09500"
     CDS             complement(1819..2208)
                     /locus_tag="PK281_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49617.1"
                     /translation="MNFKSLSGLFLLLVFCLRFLPSLEMHDHRTDRERCMQEHSDHRD
                     QFSSCELTDALGWDFSGHCSDKNHIKESNEECASCGFDFIKNFESSIGNFELVTTIDY
                     PERADFISSCYSGFTLLLCNKGPPSLT"
     gene            2679..>4781
                     /locus_tag="PK281_09505"
     CDS             2679..>4781
                     /locus_tag="PK281_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49618.1"
                     /translation="MNISLTCQPKLKSSKSQRNWLKRALFISSLFIQPILGYSQPLAS
                     RTNTAAGQTKNDPDVESLLHYFEKQIHFTENKGQWKSDILYKAEFPQGKALVTRDGVV
                     MRWFNEDDMSRHSEQSMREEEAHRDGVPFTESMTKIRSHAWKMKFLNTSEKMSIDARQ
                     KHGDQMSYFSGKGQASSVGSFQEVWYNQVYPQVDVRYYPSSEGMLEYDIICKPGFKKE
                     NIAIQLEGVSGLRLSEKGELLISTSVGEMSLPQPVVYQKVDGRQIDIPSKYLLDDKGI
                     LRFELGEYNKQLPLVIDPIALRWATWVNSNSQVVGSGKSATSGDNHGHGIWVDPTDGA
                     IYVVARVDGITNEISVGGLDNSANGGIDLVVGKYLEPATIGGSGTRVWQTYIGGSGDD
                     NPYAMEMGPDGNLYITGYTSSSDFPLIGGTAFGSSGGLDQRSQKTDNTFILKINQAGT
                     AMKAAVIGGDGDDGSFDLRLTASGDVVVCGNTTSKNLATLYASSGATNRSSGYTGNTD
                     VHLFKINQGLSSIVWMKNFGGTGSSKDVSKDQATIMAMNPTNGDIFLGGYTTSSNFPV
                     TSNARQNAIGGTQSGFIQKIKSNGTTVWSSYFKSASSKSTSILCMEFNTLKNRLYIGG
                     VTTGLDSPSNIPASGSYDNSYNSGDHDFFVASMDTNQTFVNSSYIGGSLDEVNMMGLN
                     VDLNNDVYVFGYTNSTNFP"
BASE COUNT         1447 a         1008 c          992 g         1334 t
ORIGIN      
        1 tttctctgtt tccgaatctc ccgaaatgct cagttgataa gcccgttccg attttaacgt
       61 actatcacca atctcgaaag aagcagcact ctgatgtaca ccattgccgt ataattcatt
      121 cacgttagga gctctccaag cagaacctgc atgaaatgac aaactaaact gtttcgtcca
      181 atcgtaggtt gcacccgagc taaacgaaac attgctaaaa tcgcgttgaa cagcataggt
      241 tgcaagcgtg gaataatcga ggcgataaca ttccagccaa cgataatcgt agcgcaatcc
      301 tgcttcagct tgccatttct tccattgacg tgttaaagca gcaaagacac ctgaaccata
      361 attacggaaa ttcggaatca aggctcgata ttccaatccc tgaaatacat tgccctgcgt
      421 cattaaactc aaaccagaat tcaacgtgta atgcttacct aaattagctt gataaagcaa
      481 atcggccgta tgtgtgctaa ttttgaaatc ggcttgagca gctaacatca ccgctggatc
      541 attactatag ggcaagcctg aatcatactc ttttcgacgg ttatgttgaa gtccgtatat
      601 caattcgatc tttttggtat ccgaaaaatg atagcaaact cgcgctttac ctgtgccatg
      661 aagtacagtt tgatagcctc tgtcgatttt acgctcaaat ccaacactat cgattggttt
      721 agagcttgaa aaagcagcta gcaaatcatc cacatttccc acatgtgaac ccgtaaagat
      781 tccaacctga gtgctaaaca cagaacccga aagttcgagc gctagtttct tccgattgag
      841 ctgccaactg accgaagcat cgcgttcccg cattcccgta ttggctaaat aataatgggg
      901 cgtttgaaca tttccaccat ttttcaaagt accctgaatg cgtaaaccca tgccttgcaa
      961 cttgccacca aaagtctttt cgattcttgc agaagcgcct ccttgtcggc cgttgctatt
     1021 tccaactaaa cgaacatccg ccttcaaacc cttttcggaa ctcatttcat aaggctccat
     1081 caagaccaca ccagcaatcg catcggatcc aaaacgcaag cctgccgcac ctttgaggac
     1141 ggttactttg gctacattaa atggatcgat ttcaggagca tgctctgttc cccactgctg
     1201 actttccaat cgaattccgt tattaatcaa cagaatacgg ttactgtgca agccgtgaat
     1261 gatcggtttt gaaatggttg gtcccgattg cagactgcta acaccactca cctctttcaa
     1321 catttcgccc aatggttttc ccgcattgag ttgaaatgcc gcagcttgta aggtgctgga
     1381 ttgcaatgtt cggaagccat tttcatcgct acgatcatca tggatttcta cctcatgtag
     1441 ttgttttatt tcgggtataa gttgaagatg taaatccaag gattgggtta atacaaaact
     1501 ggtatcaacc ggttgatatc ccgattgcaa tatcacgagt cggtagttac ccggacaaag
     1561 tgtattgata gaagtagaag tttttagatt aacaatacgt tctgtgcctt taaaaaataa
     1621 ctgcgcagaa actgcctgag ttgatggatt agaaatgctg agttgcaaac gactgtcgca
     1681 atcttgtgct tgaatgcttg ccaaacatgc aaagaataac agccatgcaa gcagcacttt
     1741 cctttccagg gaaaatgtga aagacatgcc tcaaataatt aggaaacctg aataccggct
     1801 gagccggaag agttgaaatc aggttaatga gggcggccct ttattacaca gcagcagggt
     1861 aaatccggaa taacagctgc taataaaatc agccctctcc ggataatcta tcgtggtaac
     1921 taattcgaaa ttaccaatgc tcgattcaaa attcttgatg aaatcaaatc cacaagaagc
     1981 acattcctca ttggattctt tgatgtggtt cttatccgaa caatggccac taaaatccca
     2041 gcctaaagca tcggttaatt cacatgaact gaattgatca cgatgatcgg aatgctcttg
     2101 catacagcgt tcccggtcgg tacgatgatc gtgcatttcc aatgatggaa ggaagcgcag
     2161 acaaaatacc agcaacagaa atagtcccga taacgacttg aagttcacgc gacaaagcta
     2221 tacaatttgt caaaatacga gttggcttta ggattaaccc aatcatattc cttgatgctt
     2281 cctgacaatg tgattttctt aaaagataat ccagttaaaa cttacacggt tatttccata
     2341 tgagttcgaa tcacatttca attctcaact taatcaacct attgatatga gtgcttattc
     2401 agcaaacata tcgatatcat aacgatcaaa acgaacagga aaatttacct gattgagttt
     2461 attcctatca ggcatttgag ctaggtgttg ataccgggta tggtttaaaa gtaacccatt
     2521 agtggtattg aggtataaaa gcccaacaaa aaaacacgga aatgctcagc tttttgacta
     2581 aaaacaagac tcgatctgga tgaaaatccg cctcacaaaa gacctacaag tcttgccagt
     2641 caagagtttc aacaaaatca aacaaattta gattgcttat gaatataagc cttacctgcc
     2701 aaccgaaatt gaaaagcagc aagagtcagc gaaactggct taagcgtgct ttgttcattt
     2761 caagcctatt tatccagccc atattgggat attcccaacc tttggcatcg cgcacgaata
     2821 ctgcagctgg acaaacgaaa aatgatccag atgttgaaag tctgcttcat tacttcgaga
     2881 aacaaattca cttcaccgaa aacaaaggtc agtggaaatc ggacattctc tataaagcag
     2941 aatttcctca aggaaaggct cttgtcaccc gcgatggtgt ggtgatgcgc tggtttaacg
     3001 aagacgacat gtctcgtcac agtgagcaaa gtatgcggga agaagaagcg catcgagatg
     3061 gagttccatt tacggagtcg atgaccaaaa tccggtctca tgcttggaaa atgaagttcc
     3121 tcaatacctc cgaaaagatg agtattgatg ctcgacagaa gcatggcgat cagatgagtt
     3181 acttttccgg aaaaggacaa gcctcgtcgg ttggtagctt ccaagaagtt tggtacaatc
     3241 aggtttatcc gcaagtggat gtacgttatt atccttcctc agaaggaatg ctcgaatacg
     3301 acatcatttg taagcctggt ttcaaaaagg aaaatattgc cattcagctc gaaggtgttt
     3361 cgggcttacg tctttctgaa aaaggcgaac tcttaatatc caccagtgtg ggtgaaatga
     3421 gtttacctca acctgtcgtg tatcaaaaag tagatggtcg tcaaatcgac ataccttcta
     3481 aatacctgtt agatgacaaa ggaattttac gtttcgaatt aggagaatac aataaacaac
     3541 tccctttagt aattgacccc attgcattgc gttgggctac ttgggtgaat tcaaattcgc
     3601 aagttgttgg ttcaggaaaa tcggctacca gtggtgataa tcacggtcac ggtatttggg
     3661 ttgatccaac ggatggagct atttatgtgg tagctcgtgt ggatgggatt acgaatgaga
     3721 tttcagttgg cggattagac aattcggcta acggtggaat tgatttagtt gttggaaaat
     3781 acttggaacc tgctacaatt ggtggttcgg gaacacgtgt ttggcaaacg tatattggtg
     3841 gttcgggtga tgataatcca tacgccatgg aaatgggacc tgatggaaat ctttatatca
     3901 ccggttatac ttcaagttct gatttcccat taattggtgg aacagctttc ggtagctcag
     3961 gcggattaga tcaacgctca caaaagacag ataatacatt catcctaaaa attaaccaag
     4021 caggtacagc catgaaagcg gctgttattg gtggtgatgg cgatgatgga tcgttcgatt
     4081 tacgtttaac tgcttcgggt gatgttgttg tatgtggaaa tacaaccagt aaaaacctcg
     4141 ctacgctata tgcatccagt ggtgcaacaa atcgtagttc gggttatact ggtaatacag
     4201 atgtgcattt gtttaaaatc aatcaaggtc tctcttcgat tgtttggatg aaaaactttg
     4261 gaggaacagg aagttccaaa gatgtttcga aagatcaagc aaccatcatg gcaatgaacc
     4321 caacaaatgg cgacattttc ttaggtggtt atacgacttc gagcaatttc ccagtaacta
     4381 gtaatgcgcg tcaaaatgct atcggtggaa cgcaaagtgg tttcatccaa aaaattaaat
     4441 cgaacggtac tactgtttgg tcttcttatt tcaaatctgc ttcgagtaaa tcgacttcca
     4501 ttttatgtat ggaattcaac acactcaaaa accgcttgta cattggtggt gtgacgacag
     4561 gtttggattc gccttcgaat attccagctt cgggttctta cgataattcg tacaacagtg
     4621 gcgaccacga tttctttgtg gcgagtatgg atacgaatca aacgtttgtc aattcatcct
     4681 atatcggtgg aagtttggat gaagtaaaca tgatgggatt aaacgtagat ctcaacaatg
     4741 atgtgtatgt atttggatat accaacagta ccaatttccc a
//
LOCUS       DAOOQW010000187         4746 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_21483_length_4746_cov_4.438926, whole genome shotgun sequence.
ACCESSION   DAOOQW010000187 DAOOQW010000000
VERSION     DAOOQW010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4746
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21483_length_4746_cov_4.438926"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..765)
                     /locus_tag="PK281_09510"
     CDS             complement(<1..765)
                     /locus_tag="PK281_09510"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HOY49619.1"
                     /translation="MDYKSLLKTSQFWRHLVVASLVAFLVLWGSFALLNVYTKHGDEV
                     VVPDFEKIYIRDLDKFVEGHNVRYEIVDSIYNLEKDKGTVVDQDPEPGSKVKPGRVIY
                     LTVNAMLNQKVAMPNLIDLSLRQASSLLETYGLKVGAKRYVEGLPPVMKQLYKGVPVK
                     AGTLIDKGSSIDLVLGRGNGGGLISVPDLLGLTLTEVRMQLSAQQLGLGKIVPDIMDN
                     DTLNARVYRQSPSHNSQEGLYGGALIDVWITNSIEKI"
     gene            complement(771..3467)
                     /locus_tag="PK281_09515"
     CDS             complement(771..3467)
                     /locus_tag="PK281_09515"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49620.1"
                     /translation="MKIKVGIVFGGRSKEREISFAGGRTVYDNLNKSLFEAVPIFVDS
                     FNNFILLNWEYVYKGSIRDFYPTPDAIPTSEGEFQIYAESLGNLNAQQQENLISTLGS
                     KIQAGDLKQHIDIAFLCLHGPYGEDGRIQGLFEYLDIPYTGSGILPSAIGINKIVQKK
                     LMLQAGFNGPKFFTLTREKWIRERHASDLLQEVKQTTGFPCVVKAPTQGSSIGVNVLQ
                     EISKEALSEAIDRSFFIRYLAVSEWATLSKTAKIEWVRTLCDIREGIGIPIRIGEAVL
                     YHPDELISWLDNQQATNPMARLLLESLDGESEIIIEGFIEGKEFSSIVIEGLDGKAIC
                     LPPTEIRKGKEVFDYRSKYLPGLSRKITPIQIPDEQIAAIRETCEKLYTTLGFGVYAR
                     IDGFISSDGKIFLNDPNTTSGMMPSSFFFHQAAEIGFNPSQFITYILYTSLRKRREEL
                     KYNGTAEILLAQLDSLMKSHEGQSNTKKRIAVVFGGYSSERHISVESGRNVYEKLSSS
                     SVFEPIPVFLSGNDEKHELFTLPINLMLKDNADDILEKIQHFSVHPIIHQIIEECKGL
                     TIKFGDIQNLKAPEQLNYQQLAERVSEVFIALHGRPGEDGALQIQLSKVGLPYNGSDA
                     DSSSITIDKYRTNEILKYNGLLVAEHFLVSEEAWNSNAEAVLQKAVLTGFPLIAKPVD
                     DGCSSAVKKIHTVEEFSSFARLIFRKQEELIESDAAKLKIKPKEEFPMKRQFLVESLI
                     SANGAETFLEITGGMLTHFDSSGNISYELFEASEALSEGEVLSLEEKFLAGQGQNITP
                     ARYAKDSKRRQEISDIVKSELEKAARALNVRGYCRIDAFVRVYANSKVEVIFIEVNSL
                     PGMTPATCIFHQSAINGYKPYDFIEKIMTFAEQSAKQKHQPA"
     gene            complement(3614..4414)
                     /locus_tag="PK281_09520"
     CDS             complement(3614..4414)
                     /locus_tag="PK281_09520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49621.1"
                     /translation="MIQSILLFLSFLLTYQSGFKHIWTVPAKGETLVTDNLGNPIVIQ
                     GDALTKFRDNGTLFRLYSNKRLGKITSVDAMNPLKIVVFYKDFSRVLFLDNTLTENGT
                     PIELEKMELEQASQVCASYDNGVWVFDGVAYTLTRFNQQMQKTVQILNLNQILNYAPQ
                     PVFMMEHDNHLYMSVPDRGILQFDIFGTYIRTIPIIGINRFQVLGTTLYYSKKPGLLQ
                     AFQIKTLQESQLELPVKDYIDMRINQDRLYLLRNDSLSVYQFPASTQE"
     gene            complement(4419..>4746)
                     /locus_tag="PK281_09525"
     CDS             complement(4419..>4746)
                     /locus_tag="PK281_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010518414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HOY49622.1"
                     /translation="YTAGPIFMEVDSNGAHEWLIEFEKAPDNLGYFTEVLDNALKSLN
                     SDYEAKRYKNITLRAPRIRTLEPGTFYYWMKQRGKLGGQNKVPRLSNDRRFLDELLQL
                     IGAGVN"
BASE COUNT         1414 a          960 c          900 g         1472 t
ORIGIN      
        1 aattttttca atagagttgg taatccaaac gtcgattaat gctcctccgt ataatccttc
       61 ttgagaattg tgagatgggc tctgacgata aacgcgtgca ttcaaggtat cattgtccat
      121 tatgtcagga acaattttac ctaagcccaa ttgttgagca ctgagttgca tcctcacttc
      181 agtaagcgtt aatcctaata aatctggtac tgaaattaat cctccaccat tacctcttcc
      241 aagaaccaaa tcaatagaag agcctttatc gataagagtt cctgctttaa ctggaacacc
      301 tttatataat tgtttcatta ctggaggaag cccttctaca taacgctttg caccaacttt
      361 caatccatag gtttcgagta aagaagatgc ttgtcttaaa gataaatcaa tcaggttggg
      421 cattgcgacc ttttgattca acatggcgtt aactgtcaag taaattactc gaccaggttt
      481 aacctttgaa cccggttccg gatcttgatc cacaactgtt cctttatctt tttcgagatt
      541 atagattgag tcaacaatct cataacgaac attatggcct tcaacaaact tatctaaatc
      601 acgtatgtaa atcttttcaa aatcaggaac aaccacctca tctccatgct tggtataaac
      661 attcagcaag gcaaatgatc cccaaagcac caaaaaggct actaaactgg caactaccag
      721 atgacgccag aattggcttg ttttgaggag tgatttatag tccattgcaa ttaggccggt
      781 tgatgtttct gtttagccga ctgttccgca aaagtcatga ttttttcaat gaaatcgtat
      841 ggtttatatc cattgattgc actctgatga aagatacagg ttgctggtgt cattccgggt
      901 aatgaattga cttcaataaa gatcacttca accttactat tagcgtatac gcgaacgaaa
      961 gcatcaatcc gacaatatcc tcttacatta agagctctcg cagctttttc gagttccgat
     1021 ttaactatat cggaaatttc ctgacgacgt ttcgaatctt tcgcataacg agcaggagtg
     1081 atattctgac cttgtccagc taagaatttt tcttccagtg atagaacttc cccttctgaa
     1141 agggcttcag aggcttcgaa taactcatag ctgatgtttc ctgaagaatc aaaatgagtg
     1201 agcattccac cagtaatttc aaggaatgtt tcagctccat ttgctgagat caggctctca
     1261 actaaaaact gacgtttcat tggaaattct tccttcggtt taatcttcaa cttagctgca
     1321 tccgattcta tcaattcttc ttgtttgcgg aaaattaatc tggcaaacga actgaactct
     1381 tctacggtgt gaattttctt cacagcagaa ctacaaccat catctaccgg tttcgctatt
     1441 aatggaaatc cagttaaaac cgctttttgt aacactgctt ctgcgttgga attccaagct
     1501 tcttcactta caagaaaatg ctccgccact aagagcccat tatatttaag gatctcgttc
     1561 gtgcgatatt tatctatcgt aatgctagaa gaatcggcat ctgagccatt gtaaggcaat
     1621 ccaactttac ttaattgaat ttgtaatgct ccatcttcac cggggcggcc atgtaaagcg
     1681 ataaaaactt ccgaaacacg ctcagccagt tgctgataat tcaattgttc aggagctttt
     1741 aaattttgaa tatccccaaa cttaattgtg agtcctttac attcttcaat tatttgatgg
     1801 attattggat gcactgaaaa gtgttggatt ttttccaaga tatcatccgc attatccttc
     1861 aacatcaagt tgataggtaa ggtgaataat tcgtgctttt catcattccc gctgaggaat
     1921 accggaattg gttcaaaaac cgaagaggaa gaaagtttct catacacatt tcgtccgctt
     1981 tctactgaaa tatgccgttc ggatgaatat cctccaaaaa caacggcaat gcgtttcttg
     2041 gtgttactct gtccttcgtg agatttcatg agagaatcaa gttgggcgag aagtatttct
     2101 gcagttccgt tgtatttcag ttcctcgcgg cgtttgcgta gggatgtata aagaatgtaa
     2161 gtgatgaatt gtgatggatt gaatccaatt tcggcagctt ggtggaagaa aaaagaggaa
     2221 ggcatcatac ccgaagtggt gttcgggtcg ttcaaaaata ttttaccatc gcttgagata
     2281 aagccatcga tacgggcata aacaccaaaa cctaaagtgg tatatagctt ttcacatgtt
     2341 tcgcgaatgg cagctatttg ctcatccgga atctgtatgg gagtgatttt gcgtgataat
     2401 ccaggtaggt attttgaacg ataatcgaac acttctttgc ctttgcgaat ttcagttgga
     2461 ggaagacaaa tagctttccc atctaaccct tcaattacaa tggaagaaaa ttctttccct
     2521 tcaataaaac cttcgatgat aatctcactt tctccatcaa gtgattcgag aagcaaacga
     2581 gccatgggat tggtcgcttg ctgattatcc agccatgaga ttaattcatc gggatgatac
     2641 aatacagctt caccaattcg aatcggaatt ccaattcctt ctcggatatc acataaagtt
     2701 cgaacccatt caatttttgc agttttgctc aaagtcgccc attcagaaac ggctaaatat
     2761 cgaatgaaga aactacgatc gatggcttct gaaagagctt ctttactaat ttcttgaagc
     2821 acatttactc ctatggatga accttgagta ggagctttta caacacaagg aaagccagtt
     2881 gtttgtttaa cttcttgaag caaatcggaa gcgtggcgtt cacgaatcca tttttcacgg
     2941 gttaaagtga agaattttgg accgttaaag cctgcttgta gcatgagctt tttttggacg
     3001 attttattga taccaatagc actaggcaat atacctgaac ccgtataagg aatgtcgagg
     3061 tattcgaaca agccttgaat acgaccatct tcgccgtatg gaccatgcaa acacaaaaat
     3121 gcaatatcga tatgctgctt taaatcacct gcttggattt tgcttcctaa agtcgatata
     3181 aggttttctt gttgctgagc attcagatta cctaaagact ctgcataaat ttgaaattca
     3241 ccctcgcttg ttggaatagc gtctggagta ggataaaaat cacgaatact tcctttataa
     3301 acgtactccc agttgaggag aatgaaatta ttgaagctat caacaaaaat gggaacagcc
     3361 tcaaataagc tcttattcag gttgtcgtaa acggttcgtc cacctgcaaa tgagatctca
     3421 cgttctttgg aacggcctcc gaagacaatg cctactttaa ttttcatgta atttgggatt
     3481 ctaaccctgt aaaactaagg ctttcatatc cctgcatcta aatagaacta ggaacttatg
     3541 aagatgttcg ggttgaaaag caaaacgtaa aattctacca attggtctgt acctaaataa
     3601 tggcaaaaga attttattcc tgtgttgaag ctggaaattg atatacacta aggctatcat
     3661 ttcttagtaa atacaagcgg tcttgattta ttcgcatgtc gatatagtcc ttaaccggaa
     3721 gttccaactg actttcttgt aatgttttta tctgaaaagc ttgaagcaaa ccgggttttt
     3781 tcgaatagta taaagttgtc cccagcactt gaaatcgatt gattcctatg attggaatgg
     3841 tgcggatata ggtcccaaaa atgtcaaatt gaagtatgcc tcgatcagga acgctcatgt
     3901 aaaggtggtt gtcgtgttcc atcataaaaa caggctgtgg agcgtaattc agtatttgat
     3961 tcaggtttag aatctgaaca gttttttgca tttgctgatt gaagcgcgta agtgtgtatg
     4021 ccactccatc aaacacccaa acaccgttat cgtaggaagc acaaacttga gaagcttgtt
     4081 cgagttccat tttctcgagc tcgataggtg ttccattttc agttaaggta ttatctaaaa
     4141 atagtacacg cgaaaaatct ttgtagaaaa caacaatctt caacggattc attgcatcta
     4201 cagaagtaat ctttcccaat cgtttattgc tgtatagccg gaacaaagtg ccattatccc
     4261 gaaatttggt taatgcatca ccttggatta caattggatt accaagatta tcggtgacta
     4321 atgtttctcc tttcgctgga acagtccaaa tatgtttgaa tccactttgg taggtgagaa
     4381 gaaatgagag aaaaagcagg atagactgga tcatggtatt agttgacgcc agcaccaatt
     4441 aattgtaaca attcatccaa gaatctacga tcattagata agcgaggaac tttattttga
     4501 ccacctagtt ttccacgttg tttcatccaa taataaaacg tacccggttc tagcgttcgt
     4561 atacgtggag cacgaagtgt gatgttttta tagcgcttcg cttcgtaatc agagtttaac
     4621 gacttgagag cattatctaa cacttcagta aagtaaccga gattatcggg agctttctca
     4681 aattcaatta gccattcatg tgctccattg ctgtcaactt ccatgaaaat tggcccagcc
     4741 gtgtat
//
LOCUS       DAOOQW010000188         4731 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_21564_length_4731_cov_4.793627, whole genome shotgun sequence.
ACCESSION   DAOOQW010000188 DAOOQW010000000
VERSION     DAOOQW010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4731
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21564_length_4731_cov_4.793627"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="PK281_09530"
     CDS             <1..476
                     /locus_tag="PK281_09530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HOY49623.1"
                     /translation="NKKAWGHNGGIDGFSSMMVWMPEDSVSIACVFNGLDMEMNDLLI
                     GLFSIYYNYPYKFPDFSRPDVVFTSDELMLYPGTYSCKDLPLKISIREESGKFYAQAT
                     GQGEFPLTAFSKIDFRFEPAGITLLFKPADPQNDRQYFTLIQAGKIYDFVKEVKE"
     gene            662..1483
                     /locus_tag="PK281_09535"
     CDS             662..1483
                     /locus_tag="PK281_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOY49624.1"
                     /translation="MTRIIAIANQKGGVGKTTTAINLAASLAILEHKTLLIDADPQAN
                     ATSGIGFDPRNIKTSIYECIINEVEPKDIILSTNTPNLDLIPAHIDLVGAEIEMINLP
                     NREHMMRAAINKISDQYEFIIIDCSPSLGLITVNSLTAADSVIIPVQCEYFALEGLGK
                     LLNTIKIVQSRLNPELEIEGILLTMYDSRLRLSNQVVEEVKTHFQQMVFDTIIQRNTR
                     LGEAPSFGLSVIMHDAGSSGAINYLNLAREILQKYDLTRMPSAEKVIEPLNENLN"
     gene            1503..2396
                     /locus_tag="PK281_09540"
     CDS             1503..2396
                     /locus_tag="PK281_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014781004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOY49625.1"
                     /translation="MNNKRNALGKGLGALLTSPETDITTKGPAPTDVAVVGSISELAV
                     DQIEANPFQPRTYFEEEALRELAESIQEMGLIQPVTVRKLGYDKYQLISGERRFRASQ
                     MAGLRTIPAYIRIANDQAMLEMALVENIQREELDSIEIALAYQRLIEEVKLTQEEVSE
                     KVAKKRSTVANYLRLLKLPPEIQLGIRERKISMGHARALVAIEDPKQQLSLYNQILSS
                     EMSVRQVEEATRKNKQPESNPARSAKAILPAELKAAINSLHKRLEVPVDIKTTQDGKG
                     QLNIRFNSPEELAQLLSKLIP"
     gene            2396..3043
                     /locus_tag="PK281_09545"
     CDS             2396..3043
                     /locus_tag="PK281_09545"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HOY49626.1"
                     /translation="MWFRLSIVYLIVRILFPISGAQAQVDTTSRGLREYKTSQGNVIS
                     KVDQAPTAKQDTVVKKAEVKPPVHSPKKAALLSAILPGAGQVYNRKYWKVGVLAAGTG
                     ALIYGLNFNQRNYTLFKGELIKRQKGEGGLDVNLEQYSNANLNELQDFYHRNRDLTLV
                     GLALVYTLNIIDATVDAHLFDFSVNDDLSMHIRPQPVYSSLLTFPAPGIGLTMKF"
     gene            3047..3781
                     /gene="dapB"
                     /locus_tag="PK281_09550"
     CDS             3047..3781
                     /gene="dapB"
                     /locus_tag="PK281_09550"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009412147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HOY49627.1"
                     /translation="MKLALIGYGGMGKAIEALAIARGHEVVLKVNKTSGSAWKDQLSL
                     ADVAIEFTQPEAAVENIYTCFEHNIPVVTGTTGWYAHLHEVSDRCLDMKQSLFYATNF
                     SIGVNMLFQMNRFLARLMATQKDYAATMEEIHHIRKKDAPSGTAITLAEGIIAEHPVY
                     DTWNRSEESTPDNSIPIHSIRIDDVPGTHHIVYQSEIDEIKLSHTAFNRQGFALGAVR
                     AAEFLHGKNGVYTMSDLLNFSMPHGL"
     gene            3771..>4731
                     /gene="lepB"
                     /locus_tag="PK281_09555"
     CDS             3771..>4731
                     /gene="lepB"
                     /locus_tag="PK281_09555"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOY49628.1"
                     /translation="MDYKWIINILLLVLLFIGLSKIFTKAGRQAWEAFVPGYNFWVWL
                     RIIDKPWWWLILLLIPGVNLMMVLIMVFLTMRNFEVESPIEIVGGIIFFPFYLIYLGF
                     GDKYRFMGADYWKKYERTKAVEWSEAIVFAVVVATIVRTFFFEAFTIPTSSMEKTLLV
                     GDFLFVSKVSYGAKTPNTPLTIPFTHHTMPFTETVPSYSEWIELPYFRFPGFGKVQNN
                     DIVVFNYPDGDTVSLNMQNISYYRLVRSLGWENVNSPEKINPYNGKPFGKVAARPVDK
                     RDNYVKRCVAIAGDKLEIKNKQLYVNDQKAQNPEMMQFSYDVQF"
BASE COUNT         1410 a          946 c          999 g         1376 t
ORIGIN      
        1 acaataaaaa agcttgggga cataatggag gaattgatgg attttcgtcg atgatggtat
       61 ggatgccgga agattcggta agcattgctt gcgtgttcaa tggcttggat atggagatga
      121 acgatttact tatcgggtta tttagtatct attataacta tccttataag ttccctgatt
      181 ttagtcggcc agatgtggtt tttacttcgg atgaattgat gttatatccg gggacttatt
      241 cgtgcaaaga tttacccttg aagattagca ttcgagaaga aagtggaaag ttttatgctc
      301 aagcaactgg acagggtgaa tttccactga cagcattttc aaaaatcgat tttcgttttg
      361 aacctgctgg aattacactt ttattcaagc cagctgatcc acaaaatgac cgacagtatt
      421 ttactttgat acaagcaggt aaaatctatg attttgtaaa agaggttaaa gaataaaaca
      481 aaatccgatt cgttttctgt ttttaggcga aaatgaggtg tgaaaaccca atttctgtta
      541 ataactagca cttaccaact actcgctgtt gatgttttcc atcccaaaaa ggagattcaa
      601 tagctatatt tgccgtgatt tcagttgaaa ccggattcaa ctgattcagt aaaaaaaaga
      661 tatgacccga ataatcgcaa tcgccaatca gaagggagga gtagggaaga cgaccactgc
      721 cataaacctg gcggccagtc tggcaattct ggaacataaa accttgttga tcgatgccga
      781 tccgcaggct aatgctactt cagggatcgg atttgatccg cgcaacatca aaacgagcat
      841 ttacgaatgc atcatcaatg aggtagaacc gaaggatatc atcctcagta ccaatacacc
      901 aaatctggat ttgattccag cgcatatcga cttggtaggt gctgaaattg agatgattaa
      961 tctaccgaac cgcgagcaca tgatgcgtgc ggcaatcaat aagattagtg atcaatacga
     1021 gttcatcatc atcgattgtt cgccatctct tggtttgatt actgtaaact ctttaacagc
     1081 tgccgattcg gttatcattc cggtacaatg tgagtatttc gccctcgaag gtttaggtaa
     1141 actcctcaat accattaaga ttgtacaatc tcgactcaat ccagaattgg aaattgaagg
     1201 aattctactc accatgtacg atagtcgttt acgcctgagt aatcaagtgg tggaagaagt
     1261 taaaacgcat ttccaacaaa tggtgttcga tactattatc caacgtaaca cccgattggg
     1321 agaagctcca agttttggat tatccgtaat tatgcacgat gcaggttcat cgggagcaat
     1381 aaattacctc aatctagctc gcgaaattct acagaaatac gatcttactc gtatgcctag
     1441 tgccgaaaag gttattgaac ctttgaacga aaatttaaat tgacaggcag ccctgaacat
     1501 acatgaataa taaacggaat gccctgggaa aaggacttgg agctttgctt acaagccccg
     1561 aaacagatat tacaaccaaa ggtccagcac cgactgatgt agctgttgtt ggctcaattt
     1621 cagaattggc ggttgatcag attgaagcaa atccatttca accacgtact tatttcgaag
     1681 aagaagcttt gcgcgaatta gcggagtcga ttcaagaaat gggacttatt caacctgtta
     1741 ccgttcggaa actaggatac gataagtatc agttgattag cggtgaacgc cgcttccgtg
     1801 cttctcaaat ggcaggtttg cgcaccattc cagcttatat ccgaattgcc aacgatcagg
     1861 caatgcttga gatggctttg gtagagaata tccaacggga agaactcgat tcaatcgaaa
     1921 tcgctttagc ctatcaacgc ttgattgaag aggtaaaact tactcaggaa gaagtgagtg
     1981 agaaagtagc caaaaagcgt tcaacagttg ccaactattt acgtttgctc aaattaccac
     2041 ccgaaattca actcggaatc cgcgaacgta aaatctctat gggacatgct cgcgcgctcg
     2101 tagctattga agatcctaag caacagctca gtttgtacaa ccagattttg agcagtgaga
     2161 tgtcggttcg acaagtggaa gaagcaacgc gcaagaacaa acagccagaa tccaatccgg
     2221 ctcgtagtgc gaaagctatc cttcctgccg aactcaaagc tgccattaat agtctgcaca
     2281 agcgtctcga agttccagtg gatatcaaaa caactcaaga cggaaaaggt cagttaaaca
     2341 ttcggtttaa ttcacctgag gaattagctc agctactcag caaacttatt ccttgatgtg
     2401 gtttcgtttg tcgatcgtct atctgattgt tcggattctt tttccgatct caggtgctca
     2461 ggcgcaggta gatacgacat caagaggact tcgtgagtat aaaacttccc aagggaatgt
     2521 aatatctaag gttgatcaag caccaactgc taaacaagat acagttgtga aaaaagctga
     2581 agtcaaacct cctgttcatt ctcctaagaa agcagcttta ttgtcggcta tattacccgg
     2641 tgctggacag gtttataatc gtaaatactg gaaagttggc gtattagcag ctggaacagg
     2701 agcgcttatt tatggcttga attttaatca gcggaattac accttgttta aaggcgaact
     2761 catcaaacga caaaaaggtg aaggcggctt agatgttaat ttggagcaat actccaatgc
     2821 taatttgaat gaattacagg atttttatca tcggaatcgt gatttaacac tagttggatt
     2881 agctttggtt tacaccttaa atattattga tgcaacggtt gatgctcatt tatttgattt
     2941 cagcgtcaac gatgatttat cgatgcatat tcgtccccaa cccgtttatt catctctttt
     3001 gacttttccg gctcccggaa ttggattaac catgaaattt taaaccatga aacttgcact
     3061 tataggttat ggaggtatgg ggaaagccat tgaagcactg gcaattgctc gtggacatga
     3121 ggtggttttg aaggtgaata aaacatctgg ttcagcttgg aaagatcaac tttcattagc
     3181 cgatgtagcc attgaattta cccaaccaga agcagctgtg gagaatatct acacctgttt
     3241 cgaacataat atccccgttg taactggcac aactggctgg tatgctcatt tacatgaagt
     3301 gagcgaccgc tgtttagaca tgaaacaaag tctgttctat gccaccaatt tcagtattgg
     3361 tgtgaatatg ttgtttcaga tgaatcgatt tcttgcacgc ttgatggcta ctcaaaaaga
     3421 ttacgctgca accatggagg aaattcacca catccgtaaa aaagatgctc ctagtggaac
     3481 tgctatcacc ttagccgaag gaatcatagc cgagcatcca gtttacgaca cttggaaccg
     3541 ttcagaagaa tcaactcccg ataattctat tcctattcat tctatccgca ttgatgatgt
     3601 tccgggaacg catcatattg tctatcaatc ggaaatagat gaaattaaat tatcccatac
     3661 agcatttaat cgacaaggat ttgcgcttgg tgctgtgcgg gccgccgaat ttctacatgg
     3721 gaaaaacggt gtttatacta tgtctgacct tttaaatttc tcaatgcctc atggattata
     3781 agtggattat aaatattctg ctgctggtac tcttgtttat cggcctatcg aaaatcttca
     3841 caaaagcagg tcgccaagct tgggaagcat ttgttcccgg atataatttc tgggtatggc
     3901 tccgtatcat tgataaacca tggtggtggt tgattttact gcttattcca ggtgtaaatc
     3961 tcatgatggt gcttatcatg gtttttctaa ccatgcgcaa cttcgaagta gaatctccta
     4021 tagaaatcgt tggagggatt attttctttc cattttacct catctaccta ggttttggag
     4081 ataagtaccg ttttatgggt gctgattatt ggaagaaata cgaacgtacc aaagctgtcg
     4141 aatggtctga ggctattgta tttgccgtgg ttgtagcaac catcgttcga acattcttct
     4201 tcgaagcctt caccattcct acttcatcta tggagaaaac cttattggtt ggagattttc
     4261 ttttcgtgag taaggtgagc tatggtgcca aaacacctaa cactccgtta acaataccat
     4321 tcacgcatca caccatgcct tttaccgaaa cggttccttc ttattccgag tggatcgaat
     4381 taccgtattt ccgttttccg ggatttggca aggttcaaaa taacgatatc gttgttttca
     4441 attaccccga tggtgatact gtttccctca acatgcagaa catcagttat taccgactcg
     4501 ttcgttcgtt gggttgggaa aatgtaaatt ctcctgaaaa gatcaaccca tacaatggaa
     4561 aaccttttgg aaaagttgca gctcgtccgg ttgataagcg agataactac gtaaaacgct
     4621 gtgtagcgat tgctggtgat aaactggaaa tcaaaaacaa gcaactatat gtgaacgatc
     4681 agaaagctca gaatccagag atgatgcagt tttcgtatga tgtgcaattt a
//
LOCUS       DAOOQW010000189         4705 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_21713_length_4705_cov_5.443441, whole genome shotgun sequence.
ACCESSION   DAOOQW010000189 DAOOQW010000000
VERSION     DAOOQW010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4705
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21713_length_4705_cov_5.443441"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..875)
                     /locus_tag="PK281_09560"
     CDS             complement(<1..875)
                     /locus_tag="PK281_09560"
                     /inference="COORDINATES: protein motif:HMM:NF039614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HOY49629.1"
                     /translation="MKYQSSNRVLMIRPASFGYNPETAASNVFQQNLGAEYSTQALEE
                     FDAMVDLLRSEGIVIWLEADQPIPAKPDAIFPNNWLGIHPGKRVVFYPMEAENRRLER
                     DPILIERLKQDLSGYSYTDWSSHELAHEFLEGTGSVVLDHIDACIYACRSSRTNARLV
                     EELGQLLNYRTHIFDSLDEQGKPYYHTNVMLSIGTQFAVICAESIPEVERAEVLDSLR
                     KSGRQLLLISRKQVAAFAGNMLEVRNSSEEKMIILSSTALDALEASEIAFLQQFALLL
                     PVDIPTIERIGGGSVR"
     gene            complement(872..3256)
                     /locus_tag="PK281_09565"
     CDS             complement(872..3256)
                     /locus_tag="PK281_09565"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HOY49630.1"
                     /translation="MLKKILLGILLLLVLLAAGLGIWLYSLKPDYSGDLNLKGIKEEV
                     QVFYDDFGVPHIYAKNEEDAYCALGYVVAQDRLFQIEMIRRLASGRLSEVLGNSLVRT
                     DRFFRTLGINRHAKWSAEEFQKRAPEAVKKSTQAYINGINQYIENGSTPIEFRILGIE
                     KEAYTLQDVFLITGYMAFGFAEGFRIDPMVEGMYRKAGEIFMKDLELGWPKESLTIPV
                     HPRVMANAEQLSQNIDHILETYPVSPWIGSNSWVLAPKKTKNGKTLLCNDTHMGYAQP
                     SIWYEAHLEYPGFHFYGNHLSGFPFALTGHSDFCGNGLTMFENDDVDFYIEQRDGNNV
                     LFNKVPTPLKMYTEVIHVKDSSDVNLQVAESTHGPLMEQVIDNFPIYNQAVSVWWSYL
                     KYPSRSLDAVYALNHARSLDDARNAVAQIDAPGLNVMYGDKDGHIAWWAAAKLVQRRP
                     GLFSKRFLDGASGADEPLGWLDFSQNPQSEDPEVGYVYSANNQPDSIVGGLYPGYYVP
                     EFRARRIVNTLSAKDGWDIEMMRKLMTDGTSDAYPAIAKELLRILETVEPVAVRSEEA
                     NRLASWEGGHGLEASGVLIYYRWIYNILRLAMRDKIGPGLFNAYMNSHLMKTSYPELL
                     KKPESVWWDIWTTKAVEKRHHVIQQAWHKTIDELIETYGKDRSKWKWSIAHTIEHVHP
                     IGRKKPFNMLFNVGPASVSGGNEVINNIGFKIDSTGYYPATFGPAMRRIIDFKDVDHS
                     WSILPTGQSGYFMADHYDDQFDLYNKNGFRPQLMDRAEIEKQADGKFLKLSPAQ"
     gene            complement(3301..4464)
                     /locus_tag="PK281_09570"
     CDS             complement(3301..4464)
                     /locus_tag="PK281_09570"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOY49631.1"
                     /translation="MRIRTVVLLVCFLASFQTKAALAKRSSRQSVVASEVEHVYYRVK
                     ARKSSNKLEGIFQKLRATYGFNGVVLITEKNHIIYKKAFGYADLQKRDSLEIGSQFQL
                     ASVSKQFTSMAIMMLKERGLLSYYDHVTRFYPDFPYPNVTIRHLLTHRAGLADYRWFI
                     DPLWPDKNKPLSNQEMMRQFAKNKPDPYFQAGSHHSYSNTGYAILAAIVEKISGVTFS
                     SFMRQAVFEPLGMKHTLVYSKCDQDCLPGNVCGYERNGRWKAPNDCFNGITGDKNVFS
                     TVDDLYKWDQALYGNQLVKQTTLAEAFKPGSPELKGWRNYGFGWRINRTNPEKLIVYH
                     SGWWRGFRTFFMRNVTDQNSIVVLSNTVNYSINSLGEVYSMVMNGELDSLAVD"
BASE COUNT         1376 a         1130 c          873 g         1326 t
ORIGIN      
        1 cgtacactac ctcctccaat gcgttctatt gttggaatat ctactggcaa taacaatgca
       61 aattgctgaa gaaatgctat ttcactcgct tctaacgcat ccaatgcagt gctcgacaaa
      121 ataatcatct tctcctccga cgaattgcga acctccaaca tatttcccgc aaatgcggcc
      181 acttgttttc gactaatcag cagtaattgt ctgcccgatt ttcgcaaaga atctaacacc
      241 tctgcacgtt ctacttcagg aatactttcg gcgcaaataa ccgcaaactg cgtcccaata
      301 ctcaacatca cattcgtgtg gtaataaggc tttccttgtt catcgagtga atcaaaaata
      361 tgtgttcggt agttcaacaa ttgacctaat tcttctacca aacgtgcatt tgttcgagac
      421 gatcggcacg cataaataca tgcatcaata tgatccaaaa ccacactgcc agttccttct
      481 aaaaattcat gagctaactc atgcgaactc caatcggtat agctatatcc cgataaatct
      541 tgctttaatc gttcaatcaa aattggatca cgttccaagc gtcgattctc agcttccata
      601 gggtaaaaaa caacccgctt tccgggatga attcccaacc agttattcgg aaaaatcgca
      661 tcaggcttcg ccggaatagg ttgatctgct tccaaccaaa taacaattcc ttccgaacgt
      721 agtaaatcta ccatggcatc aaattcttct aatgcctgag tcgaatattc tgcccccaaa
      781 ttctgctgaa acacattgct agcagctgtt tcgggattgt acccaaagct agccggacga
      841 atcatcaata cccgattgct cgattggtac ttcattgtgc cggactcagt ttcaggaatt
      901 ttccatctgc ttgcttttca atttctgcac gatccattaa ctgcggtcgg aagccgttct
      961 tattgtacag atcgaattga tcatcatagt gatcagccat aaaataaccc gattgcccag
     1021 ttggtaatat gctccaagaa tgatccacat ctttaaagtc aataatgcgt cgcatagctg
     1081 gaccaaatgt agccggataa taacccgttg aatcgatttt gaaaccaata ttattgatta
     1141 cttcattccc acccgaaaca cttgctggac caacgttaaa gagcatgtta aatggttttt
     1201 tacgtccgat cggatgcaca tgttctattg tgtgagcaat actccatttc cattttgaac
     1261 ggtctttccc atatgtttca attaactcgt caatggtctt atgccatgct tgttgtatta
     1321 catggtgacg tttttcaaca gctttggttg tccagatatc ccaccaaacc gattcaggtt
     1381 ttttcagtaa ttcgggataa gatgttttca tcaaatgcga gttcatgtag gcattgaaca
     1441 atcccggacc aattttatct cgcatcgcca aacgtaaaat attataaatc caacggtagt
     1501 aaataagtac ccccgatgct tccaatccat gaccaccttc ccaactagcc aaacgattag
     1561 cttcttccga acgaacagca acaggttcaa ctgtttccaa aatgcgtagt aattctttcg
     1621 ctatggccgg ataagcatcg ctagttccat cggtcatgag tttgcgcatc atctctatat
     1681 cccaaccatc cttagcgctt aatgtattca cgatacgtcg agctctaaat tcgggaacat
     1741 aataacccgg atacaatcca cctacaatgc tatccggctg gttattggcc gaatacacgt
     1801 agccaacttc cggatcttct gattgaggat tctgactgaa atcgagccaa cccaatggtt
     1861 catctgctcc gctagcgcca tctaagaaac gtttgctaaa caatcccggt ctacgctgaa
     1921 ccaatttagc agcagcccac caagcaatat gtccgtcttt atcaccatac attacattca
     1981 aacccggagc atcaatttga gctacagcat ttcgagcatc atccaacgaa cgtgcgtggt
     2041 tgagcgcata caccgcatcc aacgagcgag aaggatattt caaatacgac caccataccg
     2101 aaacggcttg attgtatatt ggaaaattat caatcacttg ctccatcaaa ggaccatgcg
     2161 tcgattcggc aacttgtaaa ttcacatccg aagaatcttt cacatgaatt acttcggtgt
     2221 acatttttag tggcgttggc accttgttaa acagcacatt attaccatca cgctgctcaa
     2281 tgtaaaaatc cacatcgtcg ttttcgaaca tggttaagcc attcccacaa aaatcgctat
     2341 gtccggttag ggcaaacggg aaacccgaaa gatgatttcc gtaaaaatga aatccaggat
     2401 attcgagatg cgcttcgtac caaatagaag gctgagcata gcccatgtgc gtgtcgttgc
     2461 agagaagcgt ttttccgttt ttagttttct taggagctaa aacccaactg ttagaaccaa
     2521 tccaaggtga taccggataa gtttccaaga tatgatcgat gttctgactg agttgttcag
     2581 cattcgccat cacccgcgga tgaaccggta ttgtaagact ttccttgggc cagcctaatt
     2641 caaggtcctt catgaaaatc tcaccagcct tgcgatacat tccttcaacc atcggatcaa
     2701 tacgaaatcc ttcggcaaaa ccaaaagcca tgtagccggt aattaaaaaa acatcttgta
     2761 aggtgtaggc ttctttctca atccctaaaa tccgaaattc aataggtgtg ctgccgtttt
     2821 cgatgtattg attgataccg ttaatgtatg cttgcgtact tttcttaact gcttccgggg
     2881 cacgtttctg aaactcctct gccgaccatt tggcatgacg attaattccc aaggttcgaa
     2941 aaaaacgatc agtccgcacg agcgaattac ctaacacttc cgacaatcta ccagaagcca
     3001 agcgacgaat catttcgatt tgaaacaagc gatcttgagc tactacataa ccaagagcgc
     3061 agtaagcatc ttcttcgttt ttagcgtaaa tatgcggaac accaaaatcg tcgtaaaaaa
     3121 cttgaacttc ttctttaatc cctttcaggt tgagatcgcc cgaatagtcg ggttttaaag
     3181 aatagagcca aatgcccaat ccggcagcca gtaaaacaag aaggagcaat atcccgagaa
     3241 gaatcttttt gagcatttca aacgatttgc gggggaaatt acaaaatgtt agcggtctta
     3301 ctaatcaacc gcaagagaat cgagttctcc attcatcacc atcgaataca cttcccccaa
     3361 actgttaata ctgtaattaa cggtattgga taaaacaaca atggaattct gatcggttac
     3421 atttcgcatg aaaaacgtcc gaaatccacg ccaccagcca ctgtgataca caattaactt
     3481 ttcgggatta gtacgattaa ttcgccatcc aaacccataa ttccgccagc cttttaattc
     3541 gggacttccc ggtttaaatg cttctgccaa cgtagtttgt ttcacgagtt gattcccgta
     3601 taaagcctga tcccacttat ataaatcatc tacagtcgaa aacacatttt tatcgcccgt
     3661 aatgccatta aagcaatcgt taggagcttt ccatcttccg ttgcgttcgt agccgcaaac
     3721 attccctggc aagcaatctt gatcacattt ggagtacacg agtgtatgtt tcattcccaa
     3781 tggctcaaac accgcttgac gcatgaaaga tgaaaacgtt accccgctaa ttttctcaac
     3841 aatagcagca agaatggcat atccagtatt gctatacgaa tggtgcgagc ctgcttggaa
     3901 ataaggatcg ggtttgtttt tcgcaaactg acgcatcatt tcttgattcg aaagcggctt
     3961 gtttttatcg ggccaaagtg gatcaataaa ccaacgataa tcggctaatc cagcacgatg
     4021 cgtaagtaaa tgacgaatcg taacattcgg gtaaggaaaa tcgggataga agcgcgtaac
     4081 atgatcgtag tagctcaaca agccacgttc cttgagcatc atgatagcca tcgacgtaaa
     4141 ctgtttcgac accgaagcca gctgaaactg tgaaccaatt tcgaggctgt cgcgtttttg
     4201 cagatcggcg taaccaaacg cttttttgta gatgatgtga tttttctctg taatgagcac
     4261 tacaccatta aagccatacg tggcgcgtag tttttgaaaa atcccctcga gtttgttgga
     4321 gcttttgcgc gcctttactc ggtaatacac atgctccact tcgctagcaa ccacactttg
     4381 ccgactgctc cgttttgcca gggcagcttt cgtttggaac gaagccagaa aacaaacaag
     4441 taaaacgact gtgcgtatgc gcatgaaata ggttggtata caaaactacc ttatacgcta
     4501 ggctaatcaa ttgggttgct taaataactt ttacagtagg aaatgttaac aaacgtgtag
     4561 cgattttcga tcgcttcgct ttacgatata cgatgtacga gagaaactgc taggttgaag
     4621 aattctacta agggagttac atttggaaaa gaatgcctgt ccttccttca tcttctttat
     4681 acttcgtact ttatacttaa tactg
//
LOCUS       DAOOQW010000190         4679 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_21853_length_4679_cov_4.830450, whole genome shotgun sequence.
ACCESSION   DAOOQW010000190 DAOOQW010000000
VERSION     DAOOQW010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4679
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21853_length_4679_cov_4.830450"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1577)
                     /locus_tag="PK281_09575"
     CDS             complement(<1..1577)
                     /locus_tag="PK281_09575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49632.1"
                     /translation="MILKLQKKGLAMLLVLLSIAGLQQLQAQCASTLTQWPLTTISNF
                     TYNFQAAANNMYAGEYSVFSVVAGSKYQFSLCAIDGGGGETAYDSQISLFNATNPTVP
                     IGYADDSCGSDARITWTATFTGLLNVQVNEYNCAVNQVNTTLLYRQYQSATPVNDECF
                     SAVTLSVNSSCFGTVGTLTNATGSLPTSTCEGTNTDDVWFRVTSLNTNDLEITCSPNG
                     TGFDPVMEVFTGTTCNTMGSLGCVDNGGDGVGEGANLLAPTTGQVFYVRIFDYYTALA
                     TNPNFTICAVQQPLSGGPSNNDCAGATNLSIGTVCNTITGTLLGATASSPASTCGGTN
                     TDDVWYRATATDVGVTFGVIPNGTIDAVIEIFQGNCNALSPIGCIDATVGGETEGVDV
                     TGITPGTVIYFRVYDYNSGIAADPSFGACALWETVVTAPNDECEGATTVIAGTTCGIT
                     TSSLANSTVSTPATGCAGTNNGDIWFQVFASDTAMTVEISGTDASADLVLELFVASTF
                     GDCNTLVSLGCADFTGTG"
     gene            complement(1749..2324)
                     /gene="nadD"
                     /locus_tag="PK281_09580"
     CDS             complement(1749..2324)
                     /gene="nadD"
                     /locus_tag="PK281_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HOY49633.1"
                     /translation="MQIGLYFGSYNPIHIGHLAIAGYMAQFSNLDQVWLVVSPQNPLK
                     DKQSLLQDRHRLAMVNEAIEDIPYLRSSDIEFQLPKPSYTIHTLTYLKEKYPQHQFSL
                     IMGGDNLASFHKWKNYEQIIAEHRLYVYPRPGFEPCDLLQHPSVTIIDAPLMELSSTM
                     IRQAVKQKKDVRCFMPVKAWEYMREMHFYEK"
     gene            complement(2327..2896)
                     /gene="gmk"
                     /locus_tag="PK281_09585"
     CDS             complement(2327..2896)
                     /gene="gmk"
                     /locus_tag="PK281_09585"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014791441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HOY49634.1"
                     /translation="MSGKLIIVCAPSGAGKTTIVRHILENIPGIEFSVSATNRKQRPN
                     EVNGVDYYFIDTDEFTSKIAHDEFLEWEEVYPGTFYGTLKNEIDRIWNRGNHVIFDVD
                     VEGGLNIKRHYGKRALLVFVMPPSLDVLKERLYNRATETEKSFKTRVAKAEREMEYAK
                     HADRILVNDSLVVCLPEAVSMAQSFLSQS"
     gene            complement(2893..3765)
                     /locus_tag="PK281_09590"
     CDS             complement(2893..3765)
                     /locus_tag="PK281_09590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HOY49635.1"
                     /translation="MIKSMTGFGKSQAAIGLKTLTIDIRALNSKQLDLNLRLPQAFRE
                     KEGEVRNLVSVQLERGKVDVFVSLKNSAAAAGLTLNTEAFKVRYSTLKNLADELGADT
                     SNLFSELLKSPDLMQEEETEAEDTDWTLVLEHIKEALKQVDGFRVDEGKGLGTELLDR
                     VSSIEKLLESIAVHEESRMVSVRARLKAKIDELVVQTESSANRFEEEMIYFMEKLDIS
                     EEKQRLRAHCTYFKETSAEGTPSGRKLAFISQEMGREINTIGSKANHAEMQKIVVMMK
                     DELEKIKEQLNNVL"
     gene            3868..4491
                     /locus_tag="PK281_09595"
     CDS             3868..4491
                     /locus_tag="PK281_09595"
                     /inference="COORDINATES: protein motif:HMM:NF024934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOY49636.1"
                     /translation="MASDSLQSLLPFLDPQLRIKLAETCTLMSFPADTEILREGQYIK
                     VVPLVLKGLVKVVAPIEDKELLLYYIQPAQSCVLTLSASLSNLPSNVVARTMEESQLL
                     LIPGDMLPSLLQEFPSFNRLFYELYNTRYTELVDTIRHLVFDTLDTRLMAYLQEQSTR
                     MGSSIIKFSHRQIAEDLGTSREVISRVMKKLEKLGKVVQTPDGIEIL"
BASE COUNT         1437 a         1058 c          760 g         1424 t
ORIGIN      
        1 ccagttccgg taaaatcagc acatccaagg cttaccaaag tgttgcaatc gccaaaagta
       61 gatgctacaa acaattctaa aactaaatct gcactagcat cagtacccga aatctccaca
      121 gtcatagcag tatcagatgc aaatacttgg aaccagatgt caccattatt tgtacctgca
      181 catcctgtgg ccggagtaga aacagttgaa ttggctaaag atgatgttgt gattccgcaa
      241 gttgttcctg caatgactgt agttgctcct tcacattcgt catttggagc agtaaccact
      301 gtttcccata aagcacaagc tccaaatgat ggatcagctg caattccaga attgtaatca
      361 taaactctaa agtaaattac tgtcccagga gtgatgccag ttacatctac tccttctgtt
      421 tcaccaccaa ctgttgcatc aatacaacca attggagaaa gcgcattaca atttccttgg
      481 aaaatttcaa taactgcatc gatagttcca ttgggaataa ctccaaatgt aactcccaca
      541 tcagttgctg ttgctcgata ccatacatca tctgtatttg tacctccgca agttgatgct
      601 ggagatgatg ctgttgcacc taataaagtt cctgtaatag tattacaaac tgttccgata
      661 gaaaggttag ttgcccctgc acaatcatta ttagaaggcc ctccagatag cggctgttga
      721 acagcacaaa ttgtaaagtt cgggtttgta gctaaagcag tataataatc aaatattcga
      781 acgtaaaata cttgtccagt tgttggtgcc aacaaatttg ccccttctcc tactccatca
      841 ccaccattat caacacatcc caaacttccc attgtattac aagtagttcc agtaaaaact
      901 tccattactg gatcgaagcc tgttccgttt ggtgaacaag taatttctaa atcatttgta
      961 tttaatgatg taactcgaaa ccaaacatca tctgtatttg ttccttcaca agtagaagta
     1021 ggaagcgaac ccgtagcatt agttaaagtc cctactgttc caaaacaact cgaatttacc
     1081 gataaggtta cagcactaaa acattcatca tttactggtg ttgctgattg atattgacga
     1141 tacagcaaag ttgtatttac ttgattaaca gcacaattat attcattaac ttgaacattt
     1201 aacaatccag taaaagtcgc tgtccaagtt attcgcgcat cagaaccaca tgaatcatca
     1261 gcataaccaa taggcacagt tggatttgtt gcattaaaca aagaaatctg agaatcataa
     1321 gcagtttctc ctccacctcc atcaattgca caaagtgaaa attgatattt acttccagca
     1381 actacactaa ataccgagta ttcacctgca tacatattat ttgcagcagc ctgaaaatta
     1441 taggtaaaat tagaaattgt tgttagtggc cattgtgtta atgttgatgc acattgtgcc
     1501 tgaagttgct gcaatcctgc aattgataaa agaactagca acattgctaa tcccttcttt
     1561 tgtagtttta gtatcattag gtcagattaa agggggcgca aatgtaatac acatacccca
     1621 aaagtgttaa gttgaaaatg ttaaaaatta aaatttcatt agagaagtac acctaatctc
     1681 ccaaaataat tgatactggt tattaagtca aaagaaaatt gaccctatgc ttacctaatc
     1741 aaacctgatt atttttcata aaaatgcatc tcccgcatat attcccaagc ttttacaggc
     1801 ataaaacatc ttacgtcttt tttctgctta actgcctgtc gaatcattgt agaagataac
     1861 tccatcaaag gagcatcaat tattgttacc gatggatgtt gcaaaagatc acacggttca
     1921 aatcccggtc ttggatatac atacaaccga tgctcagcta taatctgctc ataattcttc
     1981 cacttatgga agctcgccaa attatctcct cccataatca atgagaattg atgctgagga
     2041 tatttctctt tgagataagt tagtgtatgt atcgtatatg atggcttagg taactgaaat
     2101 tcaatatcgc ttgatcttaa ataaggaatg tcctcaattg cctcatttac catggctaaa
     2161 cgatgcctgt cctgaagcaa actctgcttg tctttcaatg gattttgagg cgaaacaaca
     2221 agccacacct gatctaaatt gctaaactgt gccatataac ctgcaatggc taaatgacca
     2281 atatgaattg ggttgtacga accgaagtac agtccgattt gcatgtttaa gattgtgata
     2341 aaaacgattg cgccatgctt accgcttctg gtaaacaaac gactaaacta tcattcacca
     2401 aaatacgatc ggcatgtttt gcatactcca tttcacgttc ggcttttgca acacgtgttt
     2461 tgaagctttt ttctgtttcc gtagcccgat tatataagcg ctcttttaat acatcaagcg
     2521 atggaggcat cacaaaaacc aacaacgctc gctttccata atgacgtttg atattcaatc
     2581 cgccctctac atctacatca aaaatcacat ggttcccacg attccaaatg cggtcaatct
     2641 cattctttaa ggttccataa aaagttcctg gatacacttc ttcccactcc aaaaactcat
     2701 catgtgctat tttactcgta aactcatctg tatcaataaa gtaataatct actccattga
     2761 cttcattggg acgttgcttc ctatttgtgg ccgaaacaga gaattcaatc cccggaatat
     2821 tctccaaaat atgacgcaca atggttgtct ttccagcccc gcttggcgca catacgatga
     2881 ttagtttacc actcacaaca cattattcag ttgttcctta atcttttcca attcgtcctt
     2941 catcatcaca actatcttct gcatctcagc atgattcgcc tttgatccaa ttgtgttgat
     3001 ttctctaccc atttcctgac tgataaacgc caatttccta cccgatggtg tgccctctgc
     3061 tgatgtttct ttaaaatagg tacaatgtgc tctcaaccgt tgcttttcct ccgaaatatc
     3121 caacttctcc atgaagtaaa tcatctcctc ctcaaagcga tttgcactcg attctgtttg
     3181 aacaactaac tcatcaatct ttgccttcaa tctagctcgt acagatacca tccggctctc
     3241 ttcatgcacc gcaatcgact ccaacaattt ttcaatactt gatactcgat ctaataattc
     3301 agttccaagt ccttttcctt catccacacg aaatccatca acttgcttta gagcttcttt
     3361 gatgtgttct aatactaaag tccaatcagt atcttcagct tcagtctctt cttcttgcat
     3421 caaatcgggc gacttcaata attcagaaaa caaattactt gtgtcggcac ctagttcatc
     3481 agccagattt ttcaaagtcg aataccgaac tttaaaggct tctgtattta aggtcaaacc
     3541 agcagctgca gcagagtttt taagtgatac gaaaacatcc actttgcctc gttctaattg
     3601 aacagaaacc aaatttctaa cttctccttc tttttctcgg aaagcttgag gtaagcgtaa
     3661 attcagatct aactgtttac tattgagcgc acggatatcg attgtaagtg tcttcaagcc
     3721 aatagcagcc tgacttttcc cgaaaccggt catggatttg atcatcggcg ctaaagtagt
     3781 aaaattgatg ctttagttga tgaggggatt aaaacagaaa aaactactct tacaacttct
     3841 attttagtgc taatttccat cctaacaatg gcatcagact cgcttcaatc tttactccct
     3901 tttctagatc cacagcttag aattaaactc gcagaaacct gcacgcttat gagttttcca
     3961 gccgatacgg aaatcctacg cgaaggtcaa tacatcaaag ttgttcctct cgtactgaaa
     4021 ggtttggtaa aggttgttgc tcccatcgag gacaaagaac ttctactcta ttacatccaa
     4081 ccagcgcaaa gttgcgtact aaccttaagt gcaagcctaa gcaatcttcc aagcaatgtg
     4141 gttgctcgta caatggaaga aagtcaactt ctcctcatcc ccggagatat gcttccaagc
     4201 ctgctgcaag aattcccatc gtttaaccga ctattctacg aactctataa cacacgctac
     4261 accgaactag tcgataccat tcgtcattta gtttttgaca cgctcgacac acgtttaatg
     4321 gcatatcttc aagaacaatc aactcgaatg ggttcttcaa taatcaaatt ctcgcaccgt
     4381 caaattgctg aagatttagg tacttctcgt gaggtaatta gtcgagtgat gaagaaactt
     4441 gagaaattag gaaaggttgt tcaaacacct gacggaatag aaattttgta gtggtgactt
     4501 tagtcaccga tttgaaagtt caccatgatg taggtttgtc gatacaaaag caagcataat
     4561 attctttgct gcttttctaa aaaattgtcc aactaaaaaa acacaactat gaaagcaaac
     4621 atgggttcag ccgacagaat catccgcatt attgtggcta ttgtattcgg tgtattata
//
LOCUS       DAOOQW010000191         4609 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_22271_length_4609_cov_5.336408, whole genome shotgun sequence.
ACCESSION   DAOOQW010000191 DAOOQW010000000
VERSION     DAOOQW010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4609
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22271_length_4609_cov_5.336408"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1409
                     /locus_tag="PK281_09600"
     CDS             <1..1409
                     /locus_tag="PK281_09600"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HOY49637.1"
                     /translation="FANTPFNMVISAGHSQNTATRIITLSAPELQVNMNRYYPFKRKN
                     PIGKQKEYEKIGITYTLQSKNTISQADSLYGRPGWTQKFNNGVQHQIPISTSIQFLRF
                     FTFTPAFNYNERWYFNTIEKYYDEKTGTVASDTIRGFKSTREWNASANVSTRLYGFLK
                     LGKNTLRNVMTPQVSYRYQPDFSTRRYGFYGANGAVGYYSPYENGLYGQPSVGRQGTV
                     GITLNNNLEGKFKSKRDTTGTGLKKVVLLDAFNFSTGYNLAADSLNWQNLSINARTKL
                     FKKIDVVYSSSHDFYAFDTTSNARINTIEYDKTGHLFRNLSTSLSLTTTLASGQKQKL
                     SSQAGTDEERTDLSRNPNSYVDFTIPWSLNAGFVLGKTNTGTSTKVNQVLNVSGDLNL
                     TSKWKIGFSTGYDFEVKEFSLTTIDIFRDLHCWEMHFYWIPYGFRQSYNFTINVKSSM
                     LQDLKVNRRRQWYDLQNQ"
     gene            complement(1426..2331)
                     /locus_tag="PK281_09605"
     CDS             complement(1426..2331)
                     /locus_tag="PK281_09605"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HOY49638.1"
                     /translation="MTIREAIAILELQSPVSHDDVRKAFRAKAKLYHPDRHRDERLQE
                     QASKQFISARQASEFLLNRSEAVINSPEAQRKPDPVVRRPFRTPPPPPKIVDAPIVKE
                     LDNVVKLIQLALGNKWNSHSKKFRLNLTPGSILGRWYEELIEKHYPGEDKLNGVTFAF
                     YRFFRLLFGAIFLIAAFLLMGILGLMAAVLFFPPLIVFYALYSLYSMALETVAEELNK
                     QVKRGDITSWITARYEYLWYRTIPLALFGLACWGTITFGKTGTIYLESISYLICLPVL
                     MLVLSVTYEWLHYRRIVIRQKNQSA"
     gene            2425..3369
                     /locus_tag="PK281_09610"
     CDS             2425..3369
                     /locus_tag="PK281_09610"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008240976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HOY49639.1"
                     /translation="MKLTPARTLAEVAGLINARFSGNPDHLITGINEIHKVEPGDLSF
                     VDHPKYYDKCLNSSATTILINKEVDCPEGKGLLFSDDPFRDYNFLVDYFRPFEPAKAL
                     VNSSAKIGRGTILQPGAFVGNHVVIGEDCLIHPNVVIYDHCVIGDRVVIHAGTIIGAD
                     AFYFKRRPEGYDKMKSCGRVVIGDDVEIGAGCTIDKGVTGDTVIGRGTKFDNQIHIGH
                     DTVVGEDCLFAAQVGVAGVATIENNVIIWGQVGVSKDLTIGEGAIVLAKSGVGKSLKG
                     KTTYFGIPVAEAREKMRDLANIKRIPDILDTVYNRNKE"
     gene            3394..>4609
                     /locus_tag="PK281_09615"
     CDS             3394..>4609
                     /locus_tag="PK281_09615"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HOY49640.1"
                     /translation="MIDTVKGTNDQHYRKQLYALLQVTRAINNNFPASDLFSIYEYIL
                     GHQLGFTKFAYCSFDSIWRWPICVGALHETMSIRPETDLLPFTEMTFLAEQDNPQLQN
                     FDLVVPIQHKNQPLAYLLIGELDVESMKNERKFDLEFIQTICNIIAVAIENKRLFKEN
                     IQQEMYRRELEMAREVQAMLIPSSLPNDEVIDFAAFYQPQRQVGGDYYDFIRINEDEM
                     VFCVADVSGKGVSAAILMANFQAYLRAMVINQPDLKKLVSDLNDKVVQSAKGEKFITL
                     FIAHYNQKTRQLNYVNAGHNPPILHTPNGVEILKLGTTGLGMIEKLYRLESGDVEIPP
                     KSILVCYTDGLVEQEDDSGIDFGMEHLIEFIEQHAELNSDQINSSLVKHLATFKGDQP
                     YVDDIALLTCRFH"
BASE COUNT         1409 a          922 c          951 g         1327 t
ORIGIN      
        1 cgtttgctaa tactccattt aatatggtca tcagtgctgg gcatagtcaa aacacagcta
       61 ctagaatcat cacactttcg gctcccgaat tacaagtgaa tatgaatagg tattatcctt
      121 tcaaaaggaa gaatcctatc gggaaacaaa aggaatacga gaaaattggt attacctaca
      181 cgcttcagag taaaaatacc atttctcaag ccgattcact atatggtcgc ccaggttgga
      241 ctcagaaatt caacaatggt gttcaacatc aaattcccat ttctacttcc attcagtttc
      301 ttcggttttt cacttttact ccagcattta actacaacga acgctggtat tttaatacaa
      361 tcgaaaaata ctacgacgaa aaaacaggta cggttgcatc cgatacaatc cgcgggttta
      421 aatcaacgcg tgaatggaac gccagtgcca atgtcagcac acgtttatac ggattcctca
      481 aactcggaaa aaacacacta cgcaacgtaa tgacgcctca ggtgagttat cggtatcaac
      541 ccgattttag cacgcgtcgt tatggttttt atggagcaaa tggtgcagtt ggatattatt
      601 ctccctatga aaacggattg tatggacaac cttctgtagg ccgacaagga actgttggaa
      661 ttacattaaa caacaatctc gaagggaaat tcaaatcgaa gcgtgatact actggaacag
      721 gtttgaagaa agtagtttta ctcgacgcat tcaatttttc tactggttat aatcttgctg
      781 cagattccct taactggcag aatttatcga ttaatgcacg aaccaaacta tttaagaaga
      841 ttgatgtggt ttattcgagt tcacacgatt tttatgcttt cgatacaact tcaaatgcac
      901 gaataaatac gatcgaatac gataaaacag gacatttatt tagaaacctt tctacttcct
      961 tatccttaac aaccacgctc gcatcggggc aaaagcaaaa gctcagttca caagccggaa
     1021 ccgatgagga acgtacagat ctttcacgaa atccaaacag ttacgtcgat tttaccattc
     1081 cttggtcgct aaatgcagga tttgtattgg gtaaaaccaa cacaggaact tctacgaaag
     1141 tcaaccaggt attgaatgtt agcggggatt taaatctgac ttctaaatgg aaaatcggat
     1201 ttagtactgg gtatgatttc gaagtaaaag aattttcact tactaccatt gatattttcc
     1261 gcgatctgca ttgttgggaa atgcattttt attggattcc ttatggattc agacagagtt
     1321 ataatttcac cattaacgta aaatcatcca tgctacaaga tttgaaagtt aaccgtcgtc
     1381 gccaatggta cgacttacag aatcagtaga gaaattgaca atttattaag cagattgatt
     1441 cttctggcgt atgacgattc ttctataatg aagccattcg taagtcacgc ttaaaaccaa
     1501 catcaaaacg ggaaggcaaa tcaagtagga aatagattcc aaataaatgg ttccagtttt
     1561 accaaatgta atcgttcccc aacaagccaa accaaatagt gcaagcggaa tagtccggta
     1621 ccaaagatat tcatagcgag ccgttatcca cgaagtgata tcacctcgtt ttacttgttt
     1681 atttaattct tccgcaactg tttcgagcgc catgctatac aaactatata aagcatagaa
     1741 aacgataagt ggaggaaaga ataaaacggc tgccatcaat cctaatattc ccatcaataa
     1801 aaaggcagca atgaggaaaa tagcaccaaa caacaatcgg aaaaagcggt aaaaggcgaa
     1861 tgttacacca tttaatttgt cctctcctgg ataatgtttc tcaattaatt cctcgtacca
     1921 tcttcctaat atgctacccg gcgtgaggtt caatcgaaac ttcttcgaat gagaattcca
     1981 cttatttccc aatgccaatt gaatgagctt aaccacgttg tcgagctctt taacaatagg
     2041 cgcatccaca attttaggtg gtggtggcgg agttcgaaaa gggcgtcgaa ctactgggtc
     2101 tggttttctt tgggcttcgg gcgagttaat tactgcttcc gaacggttta aaaggaactc
     2161 acttgcttgt cgtgccgata taaattgctt ggatgcttgc tcttgtaatc gttcatcacg
     2221 atgccggtcg ggatggtaca atttagcttt agcccgaaaa gccttacgca catcgtcgtg
     2281 gctaacaggt gattgcaatt caagtattgc gattgcttcg cgaattgtca tccaaaaata
     2341 aactcagctg caaaccattt gttgtatgat agcttgcaat ttcttgctgc aaatttacac
     2401 tacctttcgg cattcttctt attcatgaaa ttaacgcctg cccgcactct ggccgaagtc
     2461 gccggactaa tcaatgctcg tttctccgga aaccctgatc atctcattac aggaatcaac
     2521 gaaattcaca aagttgaacc gggagatctt tcttttgtag accatccaaa atattacgat
     2581 aaatgcctta attcttcggc tacaaccatt ctaataaata aagaagtgga ttgtcccgaa
     2641 ggaaaaggtt tgctctttag cgacgatccg tttcgggatt acaatttcct agttgattat
     2701 ttccgcccct ttgaaccagc taaagcttta gttaattctt ccgctaaaat tggtcgggga
     2761 acaattcttc aacccggtgc ttttgttgga aatcatgtgg tgattggtga agattgcctc
     2821 atccatccga atgtggttat ttacgaccat tgtgttattg gtgatcgtgt tgtgattcac
     2881 gcaggaacca tcattggtgc cgatgctttt tactttaaac gtcgccccga aggatacgat
     2941 aaaatgaaaa gttgtggacg agttgtaatt ggtgacgatg tagaaattgg agccggatgt
     3001 accatcgata aaggcgttac aggcgataca gttattggtc gaggaacgaa attcgacaat
     3061 caaattcata ttggacacga taccgtggta ggtgaagatt gcttatttgc agctcaagta
     3121 ggtgttgcag gtgttgcaac aatcgaaaac aatgtgatta tctggggaca agtaggtgtt
     3181 tcgaaagatt tgaccatagg agaaggagct attgtgttag caaaaagtgg agtagggaaa
     3241 tcactgaaag gcaaaacaac ctactttggt attcctgtag cagaagcacg tgagaaaatg
     3301 agagatttgg ctaacattaa acgtattccc gatattctcg acacagtgta taatcgcaac
     3361 aaggaataaa ttgagctttc gaaattaacg ggtgtgatag atacggttaa aggaacaaat
     3421 gatcagcatt acaggaagca gttatatgcg ctgcttcagg ttacacgtgc aatcaataac
     3481 aacttcccgg cttctgatct attcagcatt tacgagtata ttttaggaca tcaactcggg
     3541 tttaccaagt ttgcgtattg cagttttgac agtatttggc gttggccgat atgtgtgggc
     3601 gctttgcacg aaaccatgag cattcgtccc gaaaccgatt tgctcccgtt taccgaaatg
     3661 acctttttgg ccgagcagga caatcctcag ctccagaatt tcgatttagt cgttccaatt
     3721 cagcataaaa accagccttt agcttatttg cttattggtg aattggatgt ggaatcgatg
     3781 aaaaatgagc gcaagtttga ccttgagttc atccaaacca tctgcaacat catcgccgta
     3841 gcaattgaga acaaacggct cttcaaggag aatatccaac aagaaatgta tcgtcgcgaa
     3901 ttggagatgg ctcgagaagt tcaggcgatg cttattcctt cttccttgcc taacgatgaa
     3961 gtgattgatt ttgcagcatt ttatcagcct caacgccaag ttggtggaga ttactacgat
     4021 ttcattcgta tcaatgagga tgagatggtg ttttgcgtgg cagatgtttc tggtaaagga
     4081 gtttcagcag cgatcctgat ggctaatttt caagcctatc tgcgagctat ggtgatcaat
     4141 cagcctgatc tgaaaaaact cgtgagcgac ttaaacgata aagttgttca aagtgcaaaa
     4201 ggggagaagt tcatcacgtt gttcatcgca cattacaatc agaaaacacg tcagcttaat
     4261 tacgtaaacg cgggacataa tccgcccatt ttacatactc caaacggtgt tgaaatcttg
     4321 aaattgggaa ctaccggatt aggtatgatt gagaaattgt accggctcga atcgggtgat
     4381 gtggaaattc cccctaaatc gattttggta tgttataccg atggattggt tgagcaagaa
     4441 gatgattcag gaatcgactt tgggatggaa catcttatcg agtttattga gcaacacgcc
     4501 gaactcaatt cagatcaaat aaacagctca ttagtaaaac atttagccac atttaaaggt
     4561 gatcaacctt acgtggatga tatcgcttta cttacttgtc gatttcact
//
LOCUS       DAOOQW010000192         4605 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_22288_length_4605_cov_4.372527, whole genome shotgun sequence.
ACCESSION   DAOOQW010000192 DAOOQW010000000
VERSION     DAOOQW010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4605
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22288_length_4605_cov_4.372527"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /locus_tag="PK281_09620"
     CDS             complement(<1..591)
                     /locus_tag="PK281_09620"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HOY49641.1"
                     /translation="MNLKPLLSLAVTAAEEASKEILTVYHSGDFQAEAKGDNSPLTLA
                     DRKAHESIVKNLQSSKLPLLSEEGRSIPYEERKDWDYFWMVDPLDGTKEFLKRNGEFT
                     VNIALIHKGVPILGVVAVPVTGDVFYADEIAGGFVKRDGKTTPLTKRKPTDLTQSGLR
                     IVASRSHMSDETQEFVNRLNNPNLVSQGSSLKFMLLA"
     gene            complement(690..1946)
                     /gene="cysN"
                     /locus_tag="PK281_09625"
     CDS             complement(690..1946)
                     /gene="cysN"
                     /locus_tag="PK281_09625"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013070051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HOY49642.1"
                     /translation="MSTTNNQLLRFTTAGSVDDGKSTLIGRLLYDSKSIFEDQLEAVE
                     ASSAKKGFDYVDLSLLTDGLKSEREQGITIDVAYRYFATPKRKFIIADTPGHIQYTRN
                     MVTGASTANLALILIDARKGLIEQTFRHSYIASLLKIPHVIVCVNKMDLVNYDENVYS
                     KIVDDYKAFSSKLEVSDIQFVPISALLGDNVVNRSEKMDWYQGATLLHMLETVHIESD
                     YNHIDSRFPVQYVVRPMSNEHHDFRGYAGRVAGGIFRPGEEVLVQPSGFTSKIKSIRL
                     GDEILQEAFAPMSVTITLEDEIDISRGDIISKPNNHPESEQDIDVMVCWMSQRPINIG
                     SKFFVRHMTKEVKGVLKEIQYKLDINSLHKIDNADQLTLNEIARIKIRTSQPLFFDSY
                     RKNRITGSIILVDEGTNETVAAGMIV"
     gene            complement(1974..2384)
                     /locus_tag="PK281_09630"
     CDS             complement(1974..2384)
                     /locus_tag="PK281_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007093821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HOY49643.1"
                     /translation="MKSFRDLEIFIESKRLAIEVHKLSIILPKHELYEEGSQIRRSSK
                     SITSSIVEGYGRRRYKADFIKYLIYAQSECDETIVHLDFLFETGSIMDQGIYQHLKSD
                     YDSLSKRINKFIQWVEANWNNVEETKSIKPTTGN"
     gene            complement(2546..3451)
                     /gene="cysD"
                     /locus_tag="PK281_09635"
     CDS             complement(2546..3451)
                     /gene="cysD"
                     /locus_tag="PK281_09635"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015754623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HOY49644.1"
                     /translation="MNQYYLSHLKELESEAIFVIREVVAQFEKPALLFSGGKDSIVLA
                     YLSKKAFAPARIPFPLVHIDTGHNFPETMEYRDWLVNELGVKLIVGSVQESIDKGRAK
                     EETGANASRNALQTVTLLDTIEQYKYDAAMGGARRDEEKARAKERFFSHRDEFGQWDP
                     KNQRPELWNLFNGKKSMGEHFRVFPISNWTEMDVWQYIFMENIPIPSLYFSHEREVIE
                     RDGALLSTSPYLKLKPNEVPSKKIVRFRTCGDMPITGAVESDADTLEKIIEEVASSRK
                     TERGTRADDKRSETAMEDRKKAGYF"
     gene            complement(3653..4252)
                     /gene="cysC"
                     /locus_tag="PK281_09640"
     CDS             complement(3653..4252)
                     /gene="cysC"
                     /locus_tag="PK281_09640"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007647289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HOY49645.1"
                     /translation="MADHIYPIKTKVSLQARESLMNQHATLIWFTGLSGSGKSTLAVQ
                     LEAQLHALGYKTYLLDGDNIRAGINKDLSFTDEGRVENIRRIGEVSKLMLDAGIVVLS
                     AFISPFNSDREQVKSIVGSDNYIEVFVDTPLEVCEQRDVKGLYKKARAGEVKNFTGID
                     SPYEKPEHANVVIPTHDVSVEQSMEILLKHILPIIKNKV"
     gene            complement(4252..>4605)
                     /locus_tag="PK281_09645"
     CDS             complement(4252..>4605)
                     /locus_tag="PK281_09645"
                     /inference="COORDINATES: protein motif:HMM:NF013132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HOY49646.1"
                     /translation="GIIGAGKSFGPVVVLLFLFISTTFLTALITNAAAVSIVFPIAMS
                     MAEQLQMPYTPFFVAIAFAASGDFMTPIGYQTNLMVYGPGGYTFKDFIKVGWMFTIVY
                     VVVCVGFISYFYNII"
BASE COUNT         1259 a          985 c          862 g         1499 t
ORIGIN      
        1 ggccaatagc ataaacttta aactgcttcc ctgcgaaacc agattggggt tattcaacct
       61 gtttacaaat tcctgtgtct catcactcat gtgtgagcgt gatgctacaa tgcgcaagcc
      121 ggattgagtt agatcagtgg gtttgcgctt tgtgagtggt gttgtttttc catcgcgttt
      181 tacgaaacca cctgctattt catcagcgta aaaaacatcg cctgtaaccg gtaccgccac
      241 tacaccaaga atgggaacac ctttatgtat gagcgcgatg ttgactgtaa actcaccatt
      301 acgttttaaa aattcctttg taccatcaag cggatctacc atccagaaat aatcccagtc
      361 ttttctttct tcgtatggta tgcttctacc ttcctcactt aacagtggga gtttagaaga
      421 ctgtagattt tttacgattg attcgtgtgc ttttctatcc gccagcgtaa gtggagagtt
      481 atctcctttt gcttccgcct gaaaatcgcc agaatgatat accgttagaa tttcttttga
      541 ggcttcttct gcagcggtta ctgcgagaga taagaggggt tttagattca taggaattgt
      601 tcaaataata tttcatgtct gaagtttgag gtttgaagtc tcaggttcga ggttaacatc
      661 aaacttagga catcaaacct caaacggttt tacacaatca tccccgctgc cacagtttca
      721 ttcgttcctt catctaccag aataatactt cctgtaattc tgtttttgcg gtagctgtcg
      781 aagaacaaag gttgtgacgt gcggattttt atgcgggcaa tttcattcaa ggttaactga
      841 tctgcattgt caattttatg aagactgttt atatccagct tatattgaat ttcttttaat
      901 acacctttca cttctttcgt catgtgacgc acaaagaatt tggagcctat gttgattgga
      961 cgctggctca tccagcacac cattacatcg atatcttgtt cggattccgg atggttgttg
     1021 ggctttgaaa taatatcacc acggctaatg tcaatctcat cttctaatgt tattgtaacg
     1081 gacatcggtg caaatgcctc ttgtaatatt tcatctccca accgaatcga ttttattttc
     1141 gatgtaaagc cggaaggctg cactaacacc tcttctcctg gtctgaaaat tccgcctgct
     1201 actcgccctg catatccacg aaaatcgtga tgctcattcg acattggacg aacaacatac
     1261 tgcacaggga accggctatc aatatgattg taatcacttt cgatgtgaac ggtttctagc
     1321 atgtgaagca acgtagctcc ctgataccaa tccatttttt ctgaacggtt cactacgtta
     1381 tctcctagca acgcactgat gggcacaaac tgaatatcgg aaacttcaag cttggaagaa
     1441 aatgctttat agtcgtctac aattttcgaa tacacatttt catcgtagtt caccaaatcc
     1501 attttgttta cacaaacaat cacgtgcggg attttgagaa gcgatgcaat ataggagtgt
     1561 ctgaaggttt gctcgattaa tcctttgcgt gcatcaatca atatcaatgc aagattcgca
     1621 gtggatgcac ccgtaaccat gttacgcgta tattgaatat gaccaggtgt atctgcgata
     1681 ataaacttac gcttgggtgt agcgaagtag cgataggcaa catcgatggt aataccttgt
     1741 tcgcgttcgg attttaatcc atccgttaac aacgaaaggt cgacataatc aaaccctttt
     1801 tttgcactcg atgcttctac tgcttcaagc tgatcttcaa aaattgattt actgtcgtat
     1861 agcagtcttc caatgagggt actttttccg tcatctacgc tgcctgctgt tgtgaatctt
     1921 aatagttgat tatttgtggt gctcatgttt ttaaagtttc cggtttccgg tttctagttg
     1981 ccggttgttg gtttaattga tttagtttct tctacattat tccaatttgc ttcaacccat
     2041 tggataaact tgtttattct tttgctcaat gaatcataat cacttttaag atgctgatag
     2101 atcccctgat ccattataga gcctgtttca aataaaaaat caagatggac tattgtttca
     2161 tcacattctg attgagcata tatcaaatac tttataaaat ctgccttgta tcttcttctg
     2221 ccataacctt caacaattga agatgttatt gatttagaag atcttcttat ctgactacct
     2281 tcctcataaa gctcgtgctt cggaagaatg atcgataatt tatgtacttc aatagcaagc
     2341 cttttcgatt caataaatat ttccaaatct ctaaagcttt tcatacaacg ggagttgaat
     2401 tgattgccgg tttctggtta ccagttgccg gtttgttttc ataagtgatg agtttcttct
     2461 atcctaattt atattttcgt atttctttac tttattttca aagtttcaac tgcctaaccg
     2521 gaaacctgta accggcaacc agtaactaga aataccctgc tttttttctg tcttccattg
     2581 cggtttcaga acgtttgtca tcggcacggg tgccacgttc tgtttttcgt gaagacgcta
     2641 cttcctcaat tattttttct aaagtatcgg catctgattc tacagctccg gtaatcggca
     2701 tgtcaccaca ggttctgaag cgaacaattt tctttgacgg aacttcgttc ggctttagtt
     2761 tcaaataggg tgatgttgaa agtaatgcac catcacgctc aatcacttca cgctcatgcg
     2821 aaaaatacaa ggatggaata ggtatgttct ccatgaaaat gtattgccat acatccatct
     2881 cggtccagtt tgaaatgggg aatacacgga aatgttcgcc catgcttttt ttgccattga
     2941 ataaattcca caactcaggc cgctgattct ttggatccca ttgaccaaac tcatcacgat
     3001 gtgaaaagaa acgttctttc gctcttgcct tctcttcatc gcgtcttgca ccacccatag
     3061 cagcatcata cttatattgc tcgatggtat ctaataaggt aacggtttga agtgcattgc
     3121 gactggcgtt agcaccggtc tcttcttttg cacgaccttt gtcgatactt tcctgaacag
     3181 agcccacaat cagcttcaca cctaattcat ttaccagcca atcgcggtat tccatggtct
     3241 ccggaaaatt atgacccgta tcaatatgaa caagtggaaa tggaatgcgc gcgggagcaa
     3301 atgctttctt agagagatag gccaacacga tggaatcttt tccaccggaa aataacagtg
     3361 ctggcttttc gaattgtgcc actacttcgc gtatcacaaa tatggcttct gattcgagtt
     3421 cttttaaatg gcttaggtag tattggttca cgagttcgtt gttggttgtt ggatgatggt
     3481 tgttcgttgt tcgtatgtgt tcaggtcgaa agttaaaggt taatagttaa tggttaatag
     3541 tcttaaggtt tgaagtttgg tttaacaaac ccacatccca caccccaatt aacttttaac
     3601 tattatccat taactttttc aagttcaaag ttgtaggttc ttcttatatt aatcaaacct
     3661 tatttttaat aatagggagt atatgcttca ggagtatttc catcgattgt tctacgctga
     3721 catcatgtgt gggaatgacg acatttgcgt gctcaggttt ttcgtatggc gaatcaattc
     3781 ctgtaaaatt tttcacctct cctgctctcg ctttcttata taatcccttc acatcacgct
     3841 gctcgcatac ttctaatgga gtatccacaa atacctcaat gtagttgtcg ctccccacaa
     3901 ttgattttac ttgctcgcga tctgaattga atggagaaat aaatgctgac aacacaacaa
     3961 taccggcatc cagcatgagc ttcgatactt ctccaatcct tcgtatgttt tctactcggc
     4021 cttcatcggt aaacgataaa tctttattga tgcctgcccg aatattatca ccatccagca
     4081 aataggtttt atacccaagc gcatgaagct gagcttccaa ttgtactgcc aatgttgatt
     4141 tacccgaacc tgaaagaccc gtaaaccaaa ttaaggtagc atgttggttc atcaaacttt
     4201 cgcgcgcttg taaacttacc tttgttttaa taggataaat atgatctgcc attagataat
     4261 gttatagaaa taggaaataa aaccaacgca aactactaca taaacgatgg tgaacatcca
     4321 gccaacttta ataaaatcct taaatgtgta tccaccgggg ccgtatacca ttaaatttgt
     4381 ttgatatcca atgggtgtca tgaaatcacc agaggccgca aatgcaatgg ccacaaagaa
     4441 aggggtataa ggcatttgca attgttcagc cattgacatc gctatcggaa agacaataga
     4501 aacggccgct gcattggtaa tgagcgctgt taaaaatgtg gtagaaataa ataaaaaaag
     4561 caggaccaca acgggaccga atgatttacc tgcaccaata atacc
//
LOCUS       DAOOQW010000193         4603 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_22303_length_4603_cov_4.868514, whole genome shotgun sequence.
ACCESSION   DAOOQW010000193 DAOOQW010000000
VERSION     DAOOQW010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4603
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22303_length_4603_cov_4.868514"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..853)
                     /locus_tag="PK281_09650"
     CDS             complement(<1..853)
                     /locus_tag="PK281_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOY49647.1"
                     /translation="METSNKSSLKGGEFLIRESEATSVFIPEEWNEEQRMIAQSAQDF
                     LDAEVHPNLDRIDKMEPGLMHSLLTKAGELGLMGVSVPEEYGGFGKDFNTSMLVAEVA
                     GAGFSFAVALSAHTGIGTLPILYYGNEAQKQKYIPKLTTGEWKASYCLTEPGSGSDAN
                     SGKTKAKLSDDGKHYIINGQKMWITNGGFADIYTVFAKIDDDENLTAFIVERNFPGLS
                     LNEEEKKMGIKGSSTVQVFFNDCMVPVENMLSDRQNGFKIAVNILNIGRIKLAAAAIG
                     GCKQCIST"
     gene            complement(888..2066)
                     /locus_tag="PK281_09655"
     CDS             complement(888..2066)
                     /locus_tag="PK281_09655"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HOY49648.1"
                     /translation="MSTAYIVAGFRTAVAKAPRGLFRFTRPDDLAARVIEHLMQSVPE
                     LDKDRVDDVIVGNATPEAEQGLNIGRMISLMGLKTDKVPGMTVNRYCASGLETISIAT
                     AKIQAGMADCIIAGGVESMSPIPFGGWKIVPNYEVAKNNPDYYWGMGLTAEAVAREYN
                     ISREDQDAFSLESHRRAVDAIKSGRFKDDIVPVTVEEIFLDNKEKRKTRTYVVDTDEG
                     PRADSTLEMLAKLKPVFAADGVVTAGNSSQTSDGAAFVLVMSEKMVNELGLKPIARLV
                     TYQVAGVPPRIMGIGPIEAIPKALKAAGMTLDQLNLIELNEAFASQSLAIIRTLGLDT
                     NKLNVNGGAIALGHPLGCSGAKLTVQLLNELKRRDQKYGMVTMCVGTGQGAAGIFERL
                     "
     gene            complement(2546..4372)
                     /locus_tag="PK281_09660"
                     /pseudo
     CDS             complement(2546..4372)
                     /locus_tag="PK281_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020080245.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
     gene            4388..>4603
                     /locus_tag="PK281_09665"
     CDS             4388..>4603
                     /locus_tag="PK281_09665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49649.1"
                     /translation="MEEIVEEEVEEESMEDEGGDDLDLDFINEIEKAVNQEDEEEVKK
                     IFSGETILILGGEEQYRDEYGRIVDELG"
BASE COUNT         1301 a         1070 c          858 g         1374 t
ORIGIN      
        1 ctgtcgagat acattgctta cagccaccaa tggcagcagc agccaactta atccgaccta
       61 tgttcaaaat gttgactgct atcttaaatc cattctgacg atccgaaagc atattctcaa
      121 ctggaaccat gcaatcgtta aagaaaacct gaacggtaga tgagccttta attcccatct
      181 tcttctcttc ttcatttaac gataaacccg gaaaattccg ctctacgata aatgctgtta
      241 gattttcatc atcatcaatc ttggcaaaaa cagtgtatat atctgcaaaa ccaccattgg
      301 tgatccacat tttttgtccg ttgatgatat agtgttttcc atcatccgac aatttagctt
      361 tcgtttttcc gctatttgca tccgaacccg aacctggttc agttagacaa taagaagctt
      421 tccattctcc cgtagttaat tttggaatgt acttctgttt ttgagcttca tttccataat
      481 acaaaatggg aagagtccca attcctgtat gagccgaaag tgctactgca aaagaaaaac
      541 ccgctccagc aacttctgct acgagcatcg aagtattaaa gtcttttcca aatccaccat
      601 attcttcagg aaccgaaaca cccatcaatc caagctcgcc tgctttggtt aacaaggaat
      661 gcatcaggcc gggttccatt ttatcaattc ggtcgagatt aggatgaact tctgcatcta
      721 gaaaatcctg cgccgattgg gcaatcatgc gttgttcttc gttccattct tctggaataa
      781 aaaccgaagt tgcttcactc tcgcggatta aaaattcacc gccttttaag gatgatttat
      841 tgcttgtttc catgttgttg tgaattggaa tacgtggtaa aagaaaatta taatcgttcg
      901 aaaattcctg ctgcgccttg ccccgttcct acacacatgg taaccatgcc gtatttctga
      961 tcacgacgtt tcagctcatt tagaagttgc acggtaagct tggctcctga acatcccaat
     1021 ggatgcccta aagcaatcgc tccaccgttc acattcagtt tattagtatc taaaccaagt
     1081 gtgcgaataa tagccaagga ttgagaagca aatgcttcgt ttagttcaat taaattgagt
     1141 tgatctaatg tcattcccgc cgctttcaaa gcctttggaa ttgcttctat tggaccaatt
     1201 cccataatgc gtggtggaac tcctgcaact tgatacgtca ctaaacgagc aatcggtttc
     1261 aagcctaatt cattgaccat cttttccgac atcaccaata caaacgcagc accgtcggaa
     1321 gtttgagatg aattacccgc cgttacaact ccatcagctg caaatacagg ttttaattta
     1381 gccagcattt ccaatgttga atcagcgcgc ggaccttcat ctgtatctac cacataagta
     1441 cgagttttcc gtttttcttt attgtctaga aaaatctctt cgaccgttac aggaacaata
     1501 tcatccttaa atcttcccga ttttatagca tccacagctc tgcgatgaga ttccaacgaa
     1561 aatgcatcct gatcttctcg actgatatta tattctcgag ctacagcttc agcagtcaag
     1621 cccatacccc aataataatc gggattgttt ttagccactt cgtaattcgg aacaattttc
     1681 catcctccaa aaggaattgg cgacatgctc tctactccac cagcaataat acaatcagcc
     1741 attccagctt gaattttagc tgtagcgatt gatattgttt ccaatccaga tgcacaatag
     1801 cgattaaccg tcatgcccgg aaccttatcg gttttcaagc ccatgagcga aatcattcga
     1861 ccaatattaa gtccttgctc cgcctcaggt gtagcgttac caacgattac atcgtcgacc
     1921 cgatctttgt ccaattcagg caccgattgc atcaaatgtt caatcacccg cgccgccaaa
     1981 tcatccggac gagtaaaacg aaataatccc cgtggcgcct ttgccaccgc tgttcggaat
     2041 ccggctacta tgtatgctgt actcattgtt atcagtattg agtattttgt attaggtatt
     2101 aagacccttg tctaggggaa tttcgtttgt ttcgatcatt ttcttctgaa cgaattctag
     2161 attgaagtct aaaattcatt cgcagtatgt agtcaatgtc actaattagc tcatctcgtt
     2221 gatctgtagt gataaatgag caacgatagc tgagctcaat ttgaaatctt aattcgcttg
     2281 cagaaccgca cgaaatacca atgaattggt aaaactcacg atttgaattt cgaccagctc
     2341 cttcagctat gttggaacct attgaaacta ctgctcgttt catttgtgaa gacaatccat
     2401 atgattcctc tctcggaaat ccagctgtca aagaataaac tttctctgcc aactcaaggg
     2461 ctttatccca gattctcaat ttcgttacat catgcatatt gtactatttt cttgatactt
     2521 aattcttatt acttaatact atatatcagt tcctaagcac cttcccactt gtcaatatgc
     2581 tctgaacacg ttctaaggtt ttgcgctcac cgcataggct gaggaatact tcgcgctcga
     2641 gatctagtaa gtattgttcg ctcacatcgg ttggttgaga taaatcgccg ccacaaagga
     2701 cataacctag tttttcggag attttacggt catgctctga gatataatgt ccgctgaaca
     2761 tggtattagc acctgcgtag accattccaa gtcctgtgcg acctaatacg cgaatgtctt
     2821 tacgtggaac gggttgagta tatcctgctt ctgctaattc aatagcggca cgtttcgctt
     2881 cagcaattac gcgttgtgaa tttgggatga tttcgtcatg tccttttcga agaattccta
     2941 aatcatatgc ttcggtagca gatgtactaa cttttgcttg accaatggtt aagaatcgat
     3001 cgcgcaaaac atttaattgt atatcaccat cacggtaggc atctgaggca cgcagggcaa
     3061 attctttggt tccgccacca ccaggaataa gtccaacacc aaactccact aaaccaatgt
     3121 atgtttctgc agcagcaact actttatcag catgtagatt tatttcacat cctccaccga
     3181 gtgataagcc atgtggagca gtcacgacgg gaatcgatga ataacgcacg cgcatcatgg
     3241 tattttggaa tgcgcgaatg gaaaaatcca attcgtccca ttcttgttca acggccatca
     3301 taaacaacat ggctagattc gctcccgcag agaaattggg agcatcgttt ccaatcacta
     3361 atccacggta atccttttcg gcgacttcaa tggctttctg aattcccatc aaaacatcgc
     3421 ttccaatcga gttcatttta gtatgaaact ccaaattcag aatcccatcg cctaaatcac
     3481 ttaatgtggt gcctgtgttt ttccaaagtg ttttttcctg acggatatga tctagaatga
     3541 taaatgattc ataacctgga acaggaaggt agcttttaga aggaatatca taatacaatt
     3601 tctttccgcc ttctatcttg aaaaagctag tatttcctgt tgctagaaaa tcatacaccc
     3661 attgcgctgg cttcatattc atagcttcca tgcctttaac agcatctgca acaccaataa
     3721 aatcccaact ttcgaatgga ccgacttccc agccaaagcc agctttcatg gcttcatcga
     3781 tacggaaaag ctcattggca atttcaggaa tacgattcga cacataagca aataagaggt
     3841 agaaggattt gcgataaaat tctcctgctt tatcttttcc gcctaccagt actttatacc
     3901 gatcactgag tttttcaata gttttggtcg attccaaagt ggcaaatttc actttgtttt
     3961 gaggacgata ttctaaggtg tttaaatcca aagctaagat ttccgacttc cctcctgctc
     4021 ctttcgattt cttgtaaaat ccttgtccgg ttttatcacc caaccactta ttttcattca
     4081 ttttcagaac gtactcaggc aaatcgaaca acgctttttg ctcatcgttc tgacaattag
     4141 cttttaaccc atttgcaaca ttaacaagtg tgtctaaacc aactacatcg gtagttctaa
     4201 acgtagccga tttcgggcga ccaagaacgg gaccggtaag cttgtctact tcttcaacag
     4261 ttaaatccat ctcttttacg agatgaaaca gagctagaat gccaaataca ccaatgcggt
     4321 tggcaataaa tgcaggtgtg tctttcgcta gtacggtggt ttttccgagg aagagatcga
     4381 actgccgatg gaggaaattg tcgaggaaga ggttgaggaa gaatccatgg aggacgaggg
     4441 gggagatgac ctggatctcg actttatcaa cgagatcgaa aaagccgtga accaggagga
     4501 cgaggaagag gtcaagaaaa tcttcagcgg agagaccatt ttgatcctgg ggggagaaga
     4561 gcagtacaga gatgagtatg gaagaattgt cgatgaactg ggc
//
LOCUS       DAOOQW010000194         4580 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_22455_length_4580_cov_4.882652, whole genome shotgun sequence.
ACCESSION   DAOOQW010000194 DAOOQW010000000
VERSION     DAOOQW010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4580
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22455_length_4580_cov_4.882652"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="PK281_09670"
     CDS             complement(<1..338)
                     /locus_tag="PK281_09670"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOY49650.1"
                     /translation="MKIHRAIEESFRKALKPNKVLLLLGARRTGKTWFLKNILPELGL
                     PYLFLNGDDSLVQSTFQNRTVENYTRLVGNNKLLVIDEAHRIPEIGWCLKLMVDEIEG
                     LHIIATGSSV"
     gene            complement(639..959)
                     /locus_tag="PK281_09675"
     CDS             complement(639..959)
                     /locus_tag="PK281_09675"
                     /inference="COORDINATES: protein motif:HMM:NF037832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HOY49651.1"
                     /translation="MRLLLDENLPKRLKQDFIEHEIFTVADKGWTGISNGKLLTLLID
                     NNFDALLTFDKNLQYQQNFKKYTITVLVLNASDNSYETLKRLVQKIKDVLKSDLQTGP
                     IEIL"
     gene            complement(943..1185)
                     /locus_tag="PK281_09680"
     CDS             complement(943..1185)
                     /locus_tag="PK281_09680"
                     /inference="COORDINATES: protein motif:HMM:NF016167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HOY49652.1"
                     /translation="MYRIKDYITIDKEILSGNPVFMGTRVPIQTLFDHIEKGIALDEF
                     LDDFPTVKKEQAIAVIEIASKILTSKNIQQIYETAA"
     gene            complement(1271..1774)
                     /locus_tag="PK281_09685"
     CDS             complement(1271..1774)
                     /locus_tag="PK281_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013869799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2480 family protein"
                     /protein_id="HOY49653.1"
                     /translation="MEEVIVNKVANSGLINIDLEDYYIKGARRQIDITEQLFMGQILK
                     EKDFREWLKSNNWETFKGVYVAVFCSADAIIPVWAYMLIAAQLEPFAKRIVFGDLETL
                     ESELYKEIIDSLDVSEYENQRVIIKGCGNLPVPRAAFVMLTNKLRPITKSIMYGEACS
                     TVPIYKK"
     gene            complement(1786..2103)
                     /locus_tag="PK281_09690"
     CDS             complement(1786..2103)
                     /locus_tag="PK281_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly protein"
                     /protein_id="HOY49654.1"
                     /translation="MEKTLEQLEKDVIDMIRTCYDPEIPVNIYELGLIYEVNINENRD
                     VQIVMTLTSPSCPVAETLPPDVEEKIKTVEGVNSAKVEITFEPAWDQSMMSEEAKLEL
                     GFL"
     gene            complement(2108..2524)
                     /locus_tag="PK281_09695"
     CDS             complement(2108..2524)
                     /locus_tag="PK281_09695"
                     /inference="COORDINATES: protein motif:HMM:NF014688.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HOY49655.1"
                     /translation="MSIVAKEAEIIEELDMFGDWQEKFGYVIDHGKGLPAMDDQYRIE
                     ENIVHGCQNKVYIASEFDGTHVHYVADSEAQIPRGLLSIIMRVLGDEKPEDILTADLT
                     FIDKTGIRDNLSMQRSNGLEAMIRRMKMEAAKYIKN"
     gene            complement(2521..3750)
                     /locus_tag="PK281_09700"
     CDS             complement(2521..3750)
                     /locus_tag="PK281_09700"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006988848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HOY49656.1"
                     /translation="MNTLPFDVELVRRDFPILHQEVNGNPLVYFDNGATSQKPQQVID
                     AMVKYYSTYNANIHRGVHHLSQLATSAYEQAREEIRAFLNASKTEEIIFTSGTTDGIN
                     LIAQSWGRNYLKPGDEIVVTAMEHHANIVPWQLVCEQTGAVLKVIPMDTNGELILEEA
                     RKLIGLRTRMVSIIQVSNALGTINPVREILAMAREFGAMTLIDGAQAVPHFKVDVQEL
                     DCDFYVFSAHKILGPTGVGILYGKQQLLDQLPPWKGGGDMIRTVTFEKTTYNDAPLRF
                     EAGTPNISGGIVMAEGIRYWNSLNHEAVQAYEKDLLQYGTNRLKEIEGVRIIGEAKEK
                     VAVISFIMEDLHPYDIGFILDKKGIALRTGHHCAQPVMDFFGIPGTLRASFSFYNTQA
                     EIDRMVDAIGLAKKMLQ"
     gene            complement(3763..>4580)
                     /gene="sufD"
                     /locus_tag="PK281_09705"
     CDS             complement(3763..>4580)
                     /gene="sufD"
                     /locus_tag="PK281_09705"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HOY49657.1"
                     /translation="CKLPKPVQLIELVSSPEALLIQPRVLFVSDESSEAEVIQTSIST
                     KGNASIASNSVTEILVEKNAFVNWITLQSEEHTAGHFHTTEAHVKQHGKFETVTISLG
                     SGMLRNNLNIVLDAPECEAHLYGYYHPVKTQIVDNHTLVDHRYPRCESNELYKGVVDD
                     SATAIFNGKVYVRKDAQKTNAFQSNKNILLSETGTVNTKPQLEIYADDVKCSHGSSTG
                     VLDPEQLFYLRARGISLEKARVLLLNAYAGEIIEHIPHETLRNAVAEVLANRI"
BASE COUNT         1307 a         1002 c          812 g         1459 t
ORIGIN      
        1 aacacagatg aaccagttgc tataatatgc aaaccttcaa tttcatctac catcaatttc
       61 aggcaccaac caatctcagg tatgcgatgc gcttcatcaa tcacaagcag tttgttgttg
      121 cccactaaac gggtgtaatt ttcaactgtt cggttttgaa atgtggattg aactaacgag
      181 tcatctccat tcaaaaagag gtatgggaga cctaattcag gaaggatatt ctttaaaaac
      241 caagttttac cggttcttct tgcacccaat agcagcaata ctttattggg cttcaatgcc
      301 ttacgaaagc tttcttcgat agcccgatgt attttcatac accaaagcta cacatttttg
      361 atttcctatt gacgatattt aatcaaattc agtcatcata ctgacgatat ttaatcaaat
      421 tcagtcaata gccttttcgt tttgattcaa agaatcgttg cttttcttat aaatcggcac
      481 ggtagaaatg caaccgatcc tatagacaga tgctgtttta ttttcggtta aacttggaaa
      541 tgattggcta taaaccgtat taaataaaaa tgtttcgaaa cactgtctat tcggaaaact
      601 cttttaaata tagaatttaa acttttgatt ttatcaacct atagtatctc aataggtcca
      661 gtttgcaagt cagattttaa cacgtctttt atcttttgaa ctagtctttt caatgtttca
      721 taactattgt cagaagcatt taatacaaga accgttattg tatatttttt aaaattctgc
      781 tgatactgta agtttttatc aaaggttaat aaagcgtcaa aattgttatc tattaacaat
      841 gtcagtaatt ttccattaga aattcctgtc cagcctttat ctgctacagt gaaaatttca
      901 tgttcaataa agtcttgttt taatcttttg gggagatttt catcaagcag cagtctcata
      961 gatttgctgg atgttttttg aagtcaatat tttacttgct atttcgatta cagcaatggc
     1021 ttgctccttt tttacagtag gaaaatcatc aagaaattca tcaagtgcta ttcctttctc
     1081 tatatgatcg aatagggttt gaataggaac tcgtgttccc ataaaaacag gattaccgga
     1141 tagtatttcc ttgtctatag ttatatagtc tttaattcta tacatgattc aaatatacga
     1201 aaatctacca aaaacccgtc aaatgggcat tctgtgaatt atgaagtaaa aaccaatatt
     1261 taaaggctac ctatttctta taaatcggca ccgtagaaca ggcttcacca tacatgatac
     1321 ttttcgtaat cggacgaagc ttattcgtta gcatgacaaa tgccgcccgc ggaacgggga
     1381 ggttaccgca acctttgata atcacacgtt ggttttcgta ttccgaaaca tccaaactat
     1441 caataatctc tttgtataat tcgctctcca acgtttctaa atcgccaaat acaatacgtt
     1501 tagcaaatgg ctctagctga gcagcaataa gcatatatgc ccaaacggga atgatcgcat
     1561 cagccgaaca aaaaacggct acataaacac ctttgaatgt ttcccaattg ttcgacttaa
     1621 gccattcacg aaagtctttc tctttgagaa tttgtcccat gaatagctgt tcggtaatgt
     1681 caatctggcg tcgagcacct ttgatatagt agtcttccag atcaatgtta atcagtccgc
     1741 tattagctac tttattgacg attacttctt ccatcgcggt aagaattata aaaatcctaa
     1801 ttccaattta gcttcttcgc tcatcatgct ttgatcccat gctggctcga acgtgatttc
     1861 cacttttgct gaattcactc cttctaccgt tttgattttc tcttctacat cgggaggcaa
     1921 ggtttcagca accggacaag aaggtgaagt aagcgtcatc acgatctgaa catctcgatt
     1981 ctcgttaata ttcacctcgt agattaatcc caactcgtaa atattcacgg ggatttctgg
     2041 gtcataacag gtccggatca tatcgatcac atccttctca agttgttcga gtgtcttttc
     2101 catcgactca gttctttatg tatttagcag cttccatttt catacgacga atcatggctt
     2161 ccagtccatt cgaacgctgc atcgataaat tatcgcgaat gcctgtttta tctataaatg
     2221 ttagatctgc ggtcaagata tcttcgggct tttcatcacc caaaacccgc atgataatac
     2281 tcaacaatcc acgaggaatt tgagcttcac tatcagctac ataatgcaca tgtgtaccat
     2341 caaactcgga agcgatgtac actttgttct ggcatccatg aacaatattt tcttcaattc
     2401 gatactgatc gtccattgca ggcaaaccct tgccatggtc tatcacatag ccgaatttct
     2461 cttgccagtc accaaacatg tccagctcct caattatttc agcttctttc gcaacaatgc
     2521 tcattgcaac atctttttag caaggccaat ggcatcaacc attcggtcaa tttcggcctg
     2581 agtattataa aatgaaaagg atgctcgaag cgttccgggg attccaaaaa agtccattac
     2641 gggttgagcg caatgatgac cggttcgtaa tgcaattcct tttttatcga gaataaatcc
     2701 tatgtcgtag gggtgcaaat cctccattat aaaagaaatg actgctactt tctccttcgc
     2761 ctccccaata attcgaacgc cttcaatttc tttcaaacga ttggttccgt attgcaacaa
     2821 atccttttcg tatgcttgaa cagcctcgtg attgagcgaa ttccaatacc gaattccttc
     2881 cgccattaca attcccccac taatattggg tgtgccagct tcgaaacgca aaggtgcgtc
     2941 gttgtatgta gttttttcaa acgttacagt gcgaatcatg tcgccaccac ctttccaggg
     3001 cggtaattga tcgagtaatt gctgcttgcc gtataaaatt cccacgcccg ttggacctaa
     3061 aattttatgt gccgaaaaca cgtaaaaatc acaatccaac tcctgtacat ctactttaaa
     3121 atgtggaact gcttgcgctc catcaatcaa agtcatagca ccaaactcac gagccattgc
     3181 cagtatttca cgaacgggat ttatcgttcc taaagcgttc gacacctgaa taatcgacac
     3241 catgcgtgtt ctcaggccaa tcaatttgcg ggcttcttct aaaattagct caccattggt
     3301 atccatcgga atcactttca aaacggcacc cgtttgctca catactaatt gccaagggac
     3361 aatattggcg tggtgttcca tggcagttac gacaatttca tcacccggtt tcaggtagtt
     3421 tcttccccaa gattgagcga ttagattgat gccatcagta gtaccgcttg tgaagataat
     3481 ttcttcggtt ttgctggcat tcagaaatgc tcgaatttct tcccgtgctt gttcgtaagc
     3541 cgatgttgca agttgactta aatgatgcac tccacggtga atattcgcat tgtaagtcga
     3601 ataatacttc accattgcat caataacctg ttgaggtttt tgagaggttg cgccattatc
     3661 gaaatacacc agcggattgc cattaacctc ctgatgcaag attggaaaat cccggcgaac
     3721 aagctctaca tcaaaaggaa gggtattcat ggttcagatt aatcaaattc ggttggctaa
     3781 aacttcggca acagcattcc gcaatgtttc gtgtggaatg tgttcgatta tttctcctgc
     3841 gtatgcattc aagagaagta cacgagcttt ttcaagagaa attccgcgag cgcgtaggta
     3901 gaaaagttgt tctggatcta aaacacccgt agatgaacca tgcgagcatt ttacatcatc
     3961 ggcatagatt tccaattgag gcttggtatt cacggtgcca gtctcactta ggagtatatt
     4021 tttgttgctt tgaaacgcat tggtcttttg tgcatcctta cgcacataaa cctttccatt
     4081 gaatatcgct gtggcagaat catccaccac acctttatac aactcgttgc tttcgcagcg
     4141 tggataacga tgatcaacta aggtgtgatt gtctacaatt tgcgttttaa ctggatgata
     4201 ataaccgtac aaatgcgcct cacattcagg agcgtcgagc acgatattta agttattacg
     4261 caacattccc gaacctagac taatcgttac cgtttcgaat ttgccatgtt gcttcacatg
     4321 tgcttcggtt gtatgaaaat gtcccgctgt atgttcttcc gattgaagag tgatccagtt
     4381 gacaaaagcg tttttctcca ccaaaatctc cgtcacactg ttactcgcaa tagaagcatt
     4441 tcctttggtg ctgatggatg tttgaatgac ttctgcttca gatgattcat ccgaaacaaa
     4501 taatacgcga ggttgaatca aaagtgcttc tggactactt accaattcaa ttagttgaac
     4561 cggtttaggt agtttacaat
//
LOCUS       DAOOQW010000195         4559 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_22573_length_4559_cov_5.777531, whole genome shotgun sequence.
ACCESSION   DAOOQW010000195 DAOOQW010000000
VERSION     DAOOQW010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4559
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22573_length_4559_cov_5.777531"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="PK281_09710"
     CDS             complement(<1..662)
                     /locus_tag="PK281_09710"
                     /inference="COORDINATES: protein motif:HMM:NF020858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HOY49658.1"
                     /translation="MIRANDPNLRSWVPVPENSDFPIQNLPLGVICINEHHHVATRIG
                     DHVLDLAVLNKSGLLGVDFVQQIFEQDSINACMALGKEMMRDLRNRISRLLDDKHNDL
                     RKDVDLRESCLLPISQVEVCMPLKVGNYTDFYSSIEHATNVGTMLRDPANALSPNWKH
                     IPIGYHGRASSIILSGTPVKRPKGQTKAADQDAPSFGPCQQLDFELEMAFVTCRSNPL
                     GTP"
     gene            complement(697..1968)
                     /gene="glyA"
                     /locus_tag="PK281_09715"
     CDS             complement(697..1968)
                     /gene="glyA"
                     /locus_tag="PK281_09715"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOY49659.1"
                     /translation="MKKDKVIFELIDEELHRQTEGIELIASENYVSNQVMKAAGSVLT
                     NKYAEGLPGKRYYGGCEWVDEVENIAINRLKTLFNATWANVQPHSGAQANAAVMLACL
                     NPGDKILGFDLSHGGHLTHGSPVNFSGKLYQPSFYGVERETGLIDWDKVEKTAKKEKP
                     KMIICGASAYSRDWDYARLRAIADSVGALLLADISHPSGLIAKGLLNDPLPHCHIVTS
                     TTHKTLRGPRGGVIMMGQDFENPFGLKTPKGDLRMMSALLDSGVFPGTQGGPLEHIIA
                     AKAVAFHEALTDDYMNYILRVQKNAQVMAKALVKKGYNIISGGTDNHLMLIDMRSKNV
                     TGKEAEIALGKADITVNKNMVPFDDKSPFVTSGIRVGTAAITTRGITEKEIPQIVNWI
                     DEAIVNHTDDKKLAAIGKKVNKLMSDYPLFA"
     gene            2403..3350
                     /locus_tag="PK281_09720"
     CDS             2403..3350
                     /locus_tag="PK281_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013551393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HOY49660.1"
                     /translation="MNNRITKLFGIQYPIIQAGMIWCSGWELASAVSNAGGLGIIGAG
                     SMYPDVLREQIKKCQAATDKPFGVNIPLLYPNIEEHMNILVETGVKIVFTSAGNPATW
                     TSWLKERDMTVVHVIANTKFALKCEAAGVDAIVAEGFEAGGHNGREETTTLVLIPLIR
                     QAVKIPLIAAGGIGNGAQMAACLALGAEAVQVGSRFVATPESSGHEAFKQRVITSAEG
                     DTQLSLKTLTPVRLLKNSFAQNIQQAEAQGASKETLIEMLGRGRAKKGMFEGEMEEGE
                     LEIGQVSSLIREIKPAAEVVLEIMNDCKAAIQLCYQLIH"
     gene            complement(3395..4078)
                     /locus_tag="PK281_09725"
     CDS             complement(3395..4078)
                     /locus_tag="PK281_09725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49661.1"
                     /translation="MLLFVVQATKAQDIIFLKSGEEIMAKVIAINPTDIQYKKHNDKD
                     GPLYTRYKSEVFMIKYANGTKDVFTQDNNWVEPSKQIIDANHRELKAGTQINVMLVNG
                     LNSKLVSLGQTIEFQVVNDVVIDQKTVVLGGQKIQGIIVNSEKARGLGQEGKLDIQIN
                     QVLGVDGQTIPLTGILSKEGENRTTEAVVIGALVFWPVLLLKGKEAILPAGSRFMATI
                     SQTVIVSVN"
     gene            4283..>4559
                     /locus_tag="PK281_09730"
     CDS             4283..>4559
                     /locus_tag="PK281_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008238131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HOY49662.1"
                     /translation="MLAELITIGDEILIGQIVDTNSAWMAQELNLIGVKVKQITSVSD
                     DREHILAALKEASQRANVVLITGGLGPTKDDITKATMCEYFGGGLIFS"
BASE COUNT         1352 a          959 c          902 g         1346 t
ORIGIN      
        1 ggcgttccaa gcggattcga ccgacaagta acgaatgcca tttcgagctc aaaatcgagt
       61 tgttgacatg gaccaaaact aggagcatcc tgatcagcag ctttggtttg tcctttcggg
      121 cgttttacgg gtgtgccact tagtataatg gaagacgcgc gaccgtgata accgatcgga
      181 atgtgtttcc agttgggtga aagagcattt gcaggatctc gtaacatggt tcccacattg
      241 gttgcatgct cgatacttga ataaaaatcg gtgtagttac ccaccttcaa aggcatacat
      301 acttccactt gggaaatagg taacaagcag gattcgcgca aatcaacgtc tttacgtagg
      361 tcgttgtgtt tatcatcgag caaacgagaa atacgattac gcaaatcgcg catcatctct
      421 ttccctagtg ccatacaggc attaatggaa tcttgctcaa aaatttgttg cacaaaatca
      481 actccaagca atccactttt attcaacaca gctaaatcga gcacgtgatc accaattcgg
      541 gttgccacat gatgatgctc gtttatacag attacaccca aaggaagatt ctgtatggga
      601 aaatcggaat tttcaggtac cggaacccat gaacgtaaat ttggatcgtt agcgcgtatc
      661 ataaaagtta attttaaaaa gtagatcgat ttttagttaa gcaaaaagcg gataatcact
      721 catcaactta ttcactttct taccaatagc agcaagcttt ttatcatccg tatggtttac
      781 aattgcttca tcaatccagt ttacaatttg cggaatttct ttttcggtta ttccacgtgt
      841 agtgatagca gcagtcccca cacgaattcc agaagttaca aatggtgatt tatcatcaaa
      901 tggaaccatg tttttgttca cggtgatatc ggcttttcct aatgcgattt cggcttcttt
      961 accagtcaca ttcttcgaac gcatatcaat caacatcaag tgattatcag taccaccaga
     1021 aataatattg tagccttttt tcaccaaggc ctttgccatt acctgcgcat ttttctgcac
     1081 acgtaaaatg taattcatgt aatcgtcagt caaagcttcg tggaatgcta ccgctttagc
     1141 agcgataata tgttccaacg gaccaccttg agttcccgga aatacaccag aatcaagcaa
     1201 tgccgacatc atgcgtaaat ctcccttagg tgttttcaac ccaaatggat tttcgaaatc
     1261 ttgtcccatc ataataacac caccacgcgg tccacgaagg gttttatgtg tggtagatgt
     1321 tacaatatgg caatgtggaa gaggatcgtt caataaacct ttagcaatga gtccagatgg
     1381 atgcgaaata tcagctaata ataatgctcc aactgaatcg gcaatagcac gtaaacgagc
     1441 ataatcccaa tcacgactgt atgcagaagc accacaaata atcattttag gtttttcttt
     1501 tttagccgtt ttctccactt tatcccaatc aatcaaaccc gtctcacgct ctacaccata
     1561 aaaagaaggt tgatataatt taccggaaaa attcaccggt gaaccatgcg ttaaatgtcc
     1621 gccatgactc aaatcaaatc ctaaaatttt atctcccgga ttcaagcaag ctaacataac
     1681 cgcagcattt gcctgagctc ccgaatgcgg ctgaacgttt gcccaagtag cattaaacaa
     1741 agtcttcaat cgattgatgg cgatgttttc cacttcatca acccactcac atccaccata
     1801 ataacgcttg cccggaagtc cttcggcata cttgttcgtt aataccgaac cagctgcttt
     1861 catcacctga ttactcacat aattttcgga tgcaatcagc tcaattcctt cagtttggcg
     1921 atgaagttcc tcatcgatta attcaaaaat aaccttgtct tttttcatgc tttgtggttc
     1981 aaacgagggg caaaaatagt gttttggagc ggacaacacg gaatgatttt ttactaaccc
     2041 ataacaagca ttttacacat ctgtagcttc tatacaccta gcaccttcgg acttcagtta
     2101 agaatgtttt tttgtatccc aatacaagca tattacctat cagttgcatc catcccccag
     2161 catctccgga cttcagtccg gagaatattc tagcataaaa ttccaaaaga gctatttgcc
     2221 ttatgcgaac aactattatc tatactcgga ctgaagtccg agactgctag ttagagaatg
     2281 tgagtttgtg gtatgaatgc ttcgactgaa aaccgagact gctaggtaga gaatgtgaga
     2341 ttgtggtatg aatgctacga ctgaagtccg agcttgctac atttgcactt taacttcatc
     2401 ccatgaacaa tcgcatcaca aaactcttcg gaattcaata tcctattatt caagccggaa
     2461 tgatttggtg tagcggatgg gaactggctt cggcggtgag taatgcgggc ggactgggaa
     2521 ttattggcgc aggttcgatg tatccggatg tgttacggga gcaaattaag aagtgccaag
     2581 ctgctaccga taagcctttc ggggtgaata ttccactact ctaccccaac attgaggaac
     2641 acatgaatat tctggttgag acaggcgtga agatcgtttt tacttcggct ggaaatcctg
     2701 ctacctggac ttcatggctc aaagagcgcg acatgacagt cgttcacgtg attgcgaata
     2761 ccaaatttgc gctcaaatgt gaagctgctg gtgtagatgc gatagtcgca gaaggtttcg
     2821 aagcaggcgg acataatggt cgcgaagaaa ctaccacatt agttttgatt ccactcatcc
     2881 gccaggctgt taaaatccca ttgatagctg ctggaggaat tggtaatggt gctcaaatgg
     2941 cagcttgttt ggctttggga gctgaagcgg ttcaggttgg aagtcgcttt gtggctacgc
     3001 ccgaatcgtc gggacatgaa gcttttaagc agcgtgtcat tacatctgcc gaaggcgata
     3061 cgcaactttc gctgaaaaca ttaacacctg ttcgcttgtt gaagaattcc tttgcccaaa
     3121 acatccaaca ggccgaagct caaggcgcat cgaaagaaac tttaatcgaa atgctcggtc
     3181 gcggaagagc taaaaagggc atgttcgaag gtgaaatgga agaaggtgaa ctcgagattg
     3241 gacaagtaag cagcctcatt cgagaaataa aacctgctgc tgaagtcgtt ttggaaataa
     3301 tgaatgattg caaagccgcc attcaactct gctatcaact aattcactaa taaattaatc
     3361 gtaaattact tgttgaaatc tatcagtcgt agaatcaatt tacactgaca attacagttt
     3421 gacttattgt tgccataaat ctgctgccag caggtaaaat tgcttccttg cctttcagca
     3481 aaagaactgg ccaaaaaacc aaagcaccaa ttacaactgc ctcagtagtt cgattttcac
     3541 cttctttaga taaaatccca gttaatggaa ttgtttgtcc atctactcca agtacttggt
     3601 tgatttgaat atcaagcttt ccttcctgtc ctaaacctcg tgctttttca gaattgacaa
     3661 taataccttg gattttttgt cctcctaaca cgactgtttt ttggtcaatc acgacatcat
     3721 ttacaacttg aaattctatc gtttgtccaa gcgaaacaag ttttgaattc aatccattta
     3781 caagcattac attgatctga gtacctgctt ttaattctcg atgattagca tcgataatct
     3841 gctttgaagg ttctacccaa ttattatctt gagtaaaaac atccttagtc ccattcgcat
     3901 atttaatcat gaaaacttca gatttatacc gcgtatagag tggaccatct ttatcattgt
     3961 gctttttgta ttgaatatca gttgggttta tagcaatgac tttggccatt atttcttccc
     4021 ctgatttcaa aaaaattatg tcttgcgctt ttgttgcttg aacaacgaaa agaagcataa
     4081 agagaaggaa tttcatttga ggatgtagca taaagcaaaa gtagaaatat taaactagtt
     4141 tgccattcgt gttcaaaata aagagtcttg aggagtttaa aatcttaagg taatctctgt
     4201 cagaaactca gtattaaaat ccactttttg agcgttttca actgcttgta ctgtagtatt
     4261 tttatcacac aatttttagg tcatgctggc agaattaata accattggag acgaaatatt
     4321 gataggacaa atagtcgata cgaattcggc ttggatggca caggagctca atttaattgg
     4381 ggtgaaggtg aaacaaatca catctgtttc ggacgaccgg gagcatattt tagctgcttt
     4441 gaaagaagct tctcaacgag caaatgtggt tttgattacc ggtggattag gtccaacgaa
     4501 agatgatatc accaaagcta cgatgtgcga atattttgga ggtggattga tttttagtc
//
LOCUS       DAOOQW010000196         4555 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_22606_length_4555_cov_3.886667, whole genome shotgun sequence.
ACCESSION   DAOOQW010000196 DAOOQW010000000
VERSION     DAOOQW010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4555
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22606_length_4555_cov_3.886667"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            138..2558
                     /locus_tag="PK281_09735"
     CDS             138..2558
                     /locus_tag="PK281_09735"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOY49663.1"
                     /translation="MRYSYLLLLFCFIHISTIAQEIKVINGSNNKPLSFVKISVVGEE
                     KSYYTGEDGIVIVPRKWKKKEIEVMSSGFFSQKIAPNQWNKNRITISLSEVNGYDIIR
                     KVEIVATRLRAPLSSLPSDIIHLNNPASLKPTNMADALERSGEVFVQKSQLGGGSPVI
                     RGMEANRLLLVVDGVRMNTAIFRAGHLQNLLRLDPNGASNVEILAGSGSTIYGSDALG
                     GVISLNTAAAQRALGTEKSEFHSGMTFRNSSGNLQYFDQENAMNFWFNLGFRKWATRT
                     SISYSQFGDLRQGAEAVKSEWLNTTYSQSISGQDAALQNANPLIQKRSGYSQLDIQQK
                     AVYLSKNDTQHQLNFQLSASSNVNRFDRLSELSNPTTPAYSEWYYGPETRILASYQLD
                     FYKERKWFDSGRLIAAYQYNKESRVSRRFGKSSRTSRFEEVNAYSINGDWSKNLVWAK
                     LYYGFEGYYNDVTSTASSLNVLTSEESTASTRYPAGGSSVWNGAVYLNAQKTLINKII
                     LTAGVRYTVNNLEASFGTQEFYPFPFQSAQQRNNVLCGQAGIVYTPIKKLQIRAQYSQ
                     GFRSANVDDLAKVFDSNPGTLIIPNPDLLPERTETVDGSITWNQGGRFTIQAGAFYTL
                     LHDAISTEKSSLQGADSVLYDGVLSEVRSASNIQKAAIYGLHARVGLQVFANLKLNAT
                     AHYTYGSILGDTASSPLDHIPPFYGRISIDKTINKKWGASAWMIFNGEKPLSLYNVNG
                     EDNLIYATPTGMPAWQTLNFSLQLNPKEYMQLSLACENILDMNYRVFSSGISSSGRLI
                     RIQLKYNF"
     gene            2605..2961
                     /locus_tag="PK281_09740"
     CDS             2605..2961
                     /locus_tag="PK281_09740"
                     /inference="COORDINATES: protein motif:HMM:NF017880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF952 domain-containing protein"
                     /protein_id="HOY49664.1"
                     /translation="MPPPRFIYHIVLSKEWAKQATLPEYEAASLDNEGFIHCSRLEQL
                     PATLKRFFAKQLDQVSILKIDTTLINVPLIYEAADDGSGFFPHLFGGIAHRSVVEVLI
                     PPFDFMPPTPQTKADK"
     gene            complement(2924..3760)
                     /locus_tag="PK281_09745"
     CDS             complement(2924..3760)
                     /locus_tag="PK281_09745"
                     /inference="COORDINATES: protein motif:HMM:NF014658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-6-naphthoate synthase"
                     /protein_id="HOY49665.1"
                     /translation="MKIGFSPCPNDTFIFDALINKNLQPALKAEAYLADVDELNKNAI
                     AGNLEVSKLSFGVLAAVSQQYQVIDAGSALGFGCGPLLISRTPIEANAEHINPLKIAI
                     PGKSTTANLLLSMAFPEATNKEVYVFSDIEEAVLSGKVDAGLIIHENRFTYQEKGLHK
                     IIDLGAYWEERTGLPIPLGCIAVKRSLPEEEKRAIGAAIRKSVETAFAHPEGTMNYVA
                     AHAQEMQEDVMKQHIALYVNEFSISLGEKGRAAINRLMEEGEKAGLIPPICQPLFVEL
                     EA"
     gene            complement(3847..>4555)
                     /locus_tag="PK281_09750"
     CDS             complement(3847..>4555)
                     /locus_tag="PK281_09750"
                     /inference="COORDINATES: protein motif:HMM:NF019869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOY49666.1"
                     /translation="LYKLTWDDFCAWYLEAVKPAYQQPIDKATLEATKRFFEDILRLL
                     HPFTPFVTEEIWHFLAEREEGTYLMLDRQPALATFDSNIIQQFEQIRNTIAAVRNFRN
                     EKGISPKEALQLSCSSRTESASLPFMAVLTKLANISEVNYVTEKPSGSFQLMVGTDEF
                     FLPANESMDLEAEREKMEKELVYTRGFLESVMKKLQNERFMANAKPEVVEVERRKEAD
                     ALQKIKALEEQLSSLAN"
BASE COUNT         1341 a          918 c          890 g         1406 t
ORIGIN      
        1 cagattaaag tttagcgctt tgtacgggac aacacctagg tagttccgac ggatttattc
       61 agaaacttac gtgatattta aatccctttt tcatttcaaa tagaaattag taagtctatt
      121 tttgaagaaa aaaaagcatg cgttatagct acctgttatt acttttttgt ttcatacaca
      181 tctcaacaat tgctcaagaa ataaaggtaa tcaatggctc gaataataag ccactcagct
      241 ttgtaaaaat aagtgttgta ggagaagaga agagttatta tacaggagag gacggaattg
      301 ttatcgttcc aaggaaatgg aaaaaaaaag aaatcgaagt aatgagttct gggtttttca
      361 gtcagaaaat tgctccgaat caatggaata aaaatcgaat tacgatttca ctttcagagg
      421 tgaatggata tgatatcatt aggaaggtgg aaattgtggc aacgcgactt cgtgctcctt
      481 taagtagttt gccatctgat attattcatt taaataatcc agcatcactc aagccaacca
      541 atatggcaga tgccctcgaa cgaagtggtg aagtgttcgt tcagaaaagt cagttgggtg
      601 gcggtagtcc tgtcattcga ggaatggaag ccaatcgcct tttattagtg gtagatggcg
      661 ttcgcatgaa tacagctatt ttccgagctg gacatttgca aaacctctta cgtttagatc
      721 ccaatggagc ttctaatgtt gaaattttgg caggatcagg ttctaccatt tatggaagcg
      781 atgctttggg tggtgtaatt tcgttaaata cagcagctgc tcaacgtgca ttgggaacag
      841 agaaatcgga atttcattcg ggtatgacgt tccgaaattc atccggaaat ctgcagtatt
      901 tcgatcagga aaatgccatg aatttttggt tcaacctggg gtttcgcaaa tgggcaactc
      961 gcacatcaat cagctattct cagtttggtg atctacgtca aggtgccgaa gcggtgaagt
     1021 cggaatggct caatactact tattcacaaa gtatttcagg acaagatgca gccttacaaa
     1081 atgccaatcc gctcattcaa aaacgttcgg gttattcaca actcgatatt cagcaaaaag
     1141 cagtttactt atccaagaat gatacgcagc atcaactcaa ttttcagctt tctgccagca
     1201 gcaatgtaaa tcgtttcgat cgcttgtcgg agctttctaa tccaactaca ccggcctatt
     1261 cagaatggta ttatggacca gaaacacgaa tccttgcatc ttaccaactt gatttttata
     1321 aagaacgaaa atggttcgat agtggtcgcc tcattgctgc ctatcaatac aacaaagaaa
     1381 gccgtgtttc ccgccgattt ggaaaatctt ctcgcacaag tcgtttcgaa gaagtaaatg
     1441 catattctat caatggagat tggtccaaaa atttggtttg ggcaaaactc tattatggtt
     1501 ttgaaggata ttacaacgac gtaacatcaa ccgcttcatc tcttaatgtg ttaacgagtg
     1561 aagaatctac cgctagcaca cgctatcctg caggaggaag ttctgtttgg aatggtgccg
     1621 tttacctgaa tgcacaaaaa acactgatca ataaaattat tctaacggct ggtgttcgtt
     1681 atacagtaaa taatctggaa gcctcttttg gaacacaaga attttatcct ttcccttttc
     1741 aatcggcaca acaaagaaat aatgttctct gcggacaagc aggtattgta tacactccaa
     1801 taaagaaact tcaaatcaga gctcagtatt cacaagggtt tcgttcggcc aatgtggatg
     1861 atttagccaa ggtgtttgat tctaatccgg gtactttaat cattccaaat cccgatttat
     1921 taccggagcg gaccgaaacg gttgatggtt caattacttg gaatcaaggt ggaaggttca
     1981 ccattcaagc aggtgctttt tacacgctat tacacgatgc gatatcgacc gaaaaatcaa
     2041 gcttacaagg cgctgattct gttttatatg atggtgtttt gtcggaagta cgctctgcta
     2101 gtaatatcca aaaagcagca atttatgggt tacatgctcg agttggattg caagtatttg
     2161 ctaaccttaa actcaatgcc actgctcact atacgtatgg ctcaatctta ggcgatacag
     2221 catcaagtcc tttggatcat attcctcctt tttacgggcg catatcaata gataaaacaa
     2281 taaataaaaa gtggggtgct tcagcttgga tgatttttaa tggggaaaaa ccattaagtt
     2341 tatacaatgt caacggagaa gataacctga tttatgcaac cccaactggg atgcctgctt
     2401 ggcaaacact taattttagt ttgcaattga atcccaaaga atacatgcaa ctttcattgg
     2461 cttgcgaaaa tatcctcgat atgaattatc gtgttttttc aagcggaatc agctcttcag
     2521 gaagattaat tcgcattcaa ttgaaataca acttttaatg taaatgcatg tataagagtc
     2581 tgtacttttg ttgcagttta agtcatgcca cctcctcgtt ttatctatca tattgtgctg
     2641 tccaaagaat gggcaaagca agcaacattg cctgaatacg aagctgcatc tcttgataat
     2701 gaaggtttta ttcattgctc tcgattggag caattgcctg caacactcaa gcgatttttc
     2761 gctaagcaac tagatcaggt tagtatcctt aagatagata caacgcttat taatgtgccg
     2821 cttatttatg aagctgcaga tgatggttct ggcttttttc ctcatttatt tggaggaata
     2881 gctcatcgtt cagtcgttga ggttttgatt ccgccatttg attttatgcc tccaactcca
     2941 caaacaaagg ctgacaaata ggaggaatta atcctgcttt ttcaccttct tccatcaaac
     3001 gattaattgc tgctcttcct ttttctccta aggaaatact gaactcattc acataaagtg
     3061 caatgtgttg tttcattacg tcttcttgca tttcttgcgc atgagccgcc acataattca
     3121 ttgttccttc gggatgtgca aaagcagttt ctacactttt tcggattgca gctccaatgg
     3181 ctcttttttc ctcttctggg agactacgtt taactgcaat acagcccaat ggaatgggca
     3241 agccagtgcg ttcttcccaa taagctccta aatcgataat tttatgtaaa cccttttctt
     3301 ggtaggtaaa tcggttttca tgaatgatta aacccgcatc aacttttccg ctgagcactg
     3361 cttcttcaat atccgaaaac acatatactt ccttgttcgt tgcctctgga aaggccatac
     3421 ttaagaggag attcgccgtt gtgcttttac ctggaatagc aatttttaat ggattgatat
     3481 gttcagcatt agcttcgatt ggagtccttg aaataagtaa aggtccacat ccaaatccca
     3541 aagcactgcc cgcatctatc acttgatact gctgagaaac tgccgctaga acaccaaaag
     3601 aaagcttgct gacttctaaa tttccggcta tcgcattctt gtttaattca tccacatcag
     3661 ccaaataggc ttcagctttt agcgcgggct gaaggttttt attgatgagt gcatcaaaaa
     3721 taaaggtgtc gtttgggcaa ggagagaaac caattttcat agcaattcaa caagtaaaaa
     3781 ccgattgtgt tgaaataaaa agcatccgat tgttctaaaa tcagatgctt tttcatgtgt
     3841 taaatattag ttggcaagag aacttaattg ctcttccaaa gcttttattt tctgtaaagc
     3901 atctgcttct ttccggcgct ccacttctac tacttcaggt tttgcattgg ccatgaagcg
     3961 ttcgttttgc aatttcttca tcaccgattc taagaagcca cgcgtgtaaa ccaattcctt
     4021 ttccattttc tcacgctcag cctccaaatc catgctttcg ttggctggaa ggaaaaactc
     4081 atctgttcca accatcaatt ggaaagaacc acttggcttc tcagtcacat aattcacttc
     4141 tgagatattc gccaacttcg tcaaaactgc cataaaagga agtgatgcgg attcagtgcg
     4201 tgaactgcaa cttaattgaa gcgcttcttt cggtgaaata cctttttcat ttctgaaatt
     4261 acgaactgcc gcaattgtat tacggatttg ctcgaactgc tgaatgatat tcgaatcgaa
     4321 tgtcgcaagt gctggctgac gatccaacat taagtaagta ccttcctcac gttctgctaa
     4381 gaaatgccag atttcttctg tgacaaatgg tgtgaatgga tgcaataagc gaagaatatc
     4441 ttcaaagaaa cgtttggttg cttctaaagt cgctttatcg ataggttgct gataagctgg
     4501 ttttacagct tccaagtacc acgcacagaa atcgtcccaa gtgagtttgt ataat
//
LOCUS       DAOOQW010000197         4533 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_22747_length_4533_cov_5.224877, whole genome shotgun sequence.
ACCESSION   DAOOQW010000197 DAOOQW010000000
VERSION     DAOOQW010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4533
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22747_length_4533_cov_5.224877"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(343..1167)
                     /locus_tag="PK281_09755"
     CDS             complement(343..1167)
                     /locus_tag="PK281_09755"
                     /inference="COORDINATES: protein motif:HMM:NF004825.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOY49667.1"
                     /translation="MKADAVVVITGASSGIGLECAREFFRQGCRIVMASRSLKELEGI
                     QHELDPEGKRILVIQTDVTKEEECKELIQKAVGKFGRIDVLINNAGITMRALFNDVDI
                     DVLKQLMDVNFWGMVYCTKYALPYLLESKGSIVGMSSVAGYKGLPTRSGYAATKFAME
                     GFLETLRIENRRTGVHVLIARPGFVATNIRNKMMSANGTMQGVSHKDEAKSMSAAEVS
                     REIFKAVKKRKSYMILSSTAILSFWLNKFFPALVDRLVFKHVFEEKDSPLKNYHTK"
     gene            1205..2407
                     /locus_tag="PK281_09760"
     CDS             1205..2407
                     /locus_tag="PK281_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49668.1"
                     /translation="MQLIAVTDKKTNEDFYQVSEIIYKDDKNYIPHIRQDQEKIFHPD
                     KNKLFRQGGELIRWILKDDSGNLIGRVAAFIHPKTSKSGKYKVGSMGFFECIDDSEAA
                     KLLIQTCESWLKERGMEGMDGPVNFGERDQFWGLLVENFTAPPSYTMNYNPPYYQKFF
                     EDYGFKNYFNQYVYWRDLALPAQEIFVKKANILSHDPEFRVTHMRGVKTEKIAEYFLE
                     VYNSAWGGHEGFKNMRIEQARSIINSMKVIMDPDILLFAFLKDKPIGFYLNIPELNEF
                     FKHVNGNLNWWGKLKFFYHLKFSKRKTMLGIVFGVSREHQGRGVEGALISYAGDYIVP
                     QKRYTETILTWIGDFNPKMLRVIENLGTSLYRTLVTYRIYFDPSIPFERHPILGGKNN
                     SEEHTDNE"
     gene            2526..2601
                     /locus_tag="PK281_09765"
     tRNA            2526..2601
                     /locus_tag="PK281_09765"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2559..2561,aa:Lys,seq:ttt)
     gene            2722..4155
                     /locus_tag="PK281_09770"
     CDS             2722..4155
                     /locus_tag="PK281_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOY49669.1"
                     /translation="MNENRRLSAKEKALKINLDTRLYGSFAEIGAGQETASHFFRAGG
                     ASGTVAKTMSAYDMRFSDAIYGKTNRYVCEERLLQMLNKEYGLLGKRLPFRKDSTNFF
                     AFANTVETTNFQRTNQGHGWIGVRFQLNPASEPNECIIHIVLKDNENTWQQRAIGQIG
                     VNLIFGCFYQTQEPDNLVKSLLDGIIPGTVEVDMIRLTGPDLQHIDNRLLSLKLVKYG
                     LTKTAMLGPDGSVLQPSEALYKKNALVMSGRFRPFTKTHEDMLNRATADFVEELGDEN
                     HLIVLSELSLNALIDANGDINDEDFLDRANMLCSLGHTVLISNFLDYWYLVPYLAKTT
                     RNQLVGFVLSVRNMERIFDPSQYTNLTGGILEALTMLFGSNVKAYAYPAYRRTTGEII
                     TLDSLQIAPEYQGLLRYLLDNNKIEDIRNADLSVLHINADEVLNQIKSAEQGWEKFVP
                     VRVAEIIKSNSLFDFPGDSESQLINKN"
     gene            4159..>4533
                     /locus_tag="PK281_09775"
     CDS             4159..>4533
                     /locus_tag="PK281_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOY49670.1"
                     /translation="MGFETFGFSQELLDGLDAMNFREPTPVQSQAIPEIMAGKDLIAS
                     AQTGTGKTAAFLLPIIESNLLNQTHHIDTLIIVPTRELGLQIDQQLEGFAYFVPVSSI
                     AVYGGGDGKEFEVQKRALREGTN"
BASE COUNT         1407 a          941 c          833 g         1352 t
ORIGIN      
        1 acacatgtaa gttgatgttc ttttatcact taaatagcat atgtttttta ttgtcatacc
       61 tgattttact gagaaaattc aatttctagt tagattacat ttatgtaagt aaatcatcaa
      121 tcagttcctt aattggtgta ttcagaaata aatgcgattc agaaaaatct attacgtttt
      181 taagcttctt ggattctgtc attttgatct aaatctcgat taaggcaaaa aaaaagagcg
      241 gtaaaaccgc tcttctttca cataaaccac aataaacatg aataatttaa tcacttcact
      301 tgcctgaagt tatcctgtat actatacatt aaaaacaagg tttcatttag tatgataatt
      361 ctttaaagga gaatcttttt cctcaaacac atgcttaaat accaagcgat ctacaagcgc
      421 tggaaaaaat ttatttagcc aaaaagatag gattgcagta gacgataaaa tcatatatga
      481 cttacgcttt ttcactgctt tgaatatttc tcttgaaact tcagctgccg acatcgactt
      541 tgcctcatcc ttatgcgata caccttgcat agttccgttt gccgacatca tcttgtttcg
      601 aatattcgtt gctacaaaac cgggacgagc aatcagaaca tgaactcctg ttcttctatt
      661 ttcaattctt aaggtttcta aaaaaccctc catggcaaat ttagttgcag catatccgct
      721 tcgggttggt aatcctttat aaccagcaac cgacgacatt ccaacaatag atcctttaga
      781 ttctaacaaa tagggtaaag catattttgt acaatatacc atcccccaaa aattaacatc
      841 catcaactgt ttcaatacat caatgtcaac atcgttaaat aatgcacgca tggtaattcc
      901 cgcattatta atcaacacat caattcgccc aaatttaccc acagctttct ggatgagctc
      961 tttacactcc tcttcttttg tcacatctgt ttgtatgact agaatccgtt ttccttccgg
     1021 atctaattca tgttgaattc cttccagttc ttttaaactg cgtgaagcca taacaatcct
     1081 gcatccctga cggaaaaact cccgggcaca ttctagtcct attccggacg aagctcctgt
     1141 gattacgact acagcatctg ccttcatctg aaacctgtat ttttggcaaa aatagactaa
     1201 gcctatgcag ctaattgctg ttaccgataa aaaaacgaat gaggactttt atcaagtctc
     1261 cgaaatcatt tataaggacg acaaaaacta cattcctcat atacgtcaag accaggaaaa
     1321 aatttttcac ccggataaaa ataagctatt ccgtcagggc ggtgaattaa ttcgttggat
     1381 attaaaagac gactcaggca atctgattgg aagagttgcg gccttcatac atccgaaaac
     1441 ttcaaaatcg ggaaaatata aagttggaag tatgggtttc ttcgagtgta ttgatgactc
     1501 ggaagcagcc aaactactaa ttcaaacatg cgaatcttgg ttaaaagaac gcggaatgga
     1561 aggtatggat ggccctgtga attttggcga acgagatcaa ttttgggggc ttctggtaga
     1621 gaatttcacc gcgcctccta gctacacgat gaattataat cctccttact accaaaagtt
     1681 tttcgaagat tatggattca aaaattactt caaccaatat gtttactggc gtgatttagc
     1741 attgccagca caggaaatat ttgtcaaaaa agccaacatt cttagtcacg accccgagtt
     1801 cagagtaaca cacatgcgag gagtgaagac agaaaaaatt gccgaatact ttctagaagt
     1861 atacaattcg gcttggggcg gacatgaagg ctttaaaaat atgcgaatcg aacaggctcg
     1921 ctccattatc aattcaatga aagtgattat ggatcctgat attcttttgt tcgctttctt
     1981 aaaagataaa ccgattggct tctacctcaa cattcctgaa ctaaacgagt tcttcaaaca
     2041 tgtgaacgga aaccttaatt ggtggggaaa actcaagttc ttctatcact tgaaatttag
     2101 taaacgtaaa accatgctcg gaatcgtatt tggcgtttcg cgtgaacacc aaggtagagg
     2161 tgtggaaggt gctctcatta gctatgcggg cgattatatc gtgcctcaaa aacgttacac
     2221 cgaaacaatt ttaacctgga ttggtgattt caatcctaag atgcttcgtg ttatcgaaaa
     2281 cctcggaacc tcgctctaca gaacgcttgt aacgtatcgg atctattttg acccaagcat
     2341 acctttcgaa cgacatccta ttttaggagg aaaaaataat tcagaagaac atactgataa
     2401 tgaatagtct agaaatgaat cgctagaaat atgagatttt tcgaatcttt tattttgctc
     2461 aaaccgaaat cgacttatat ttgcacccgc tttcccggta agcttgcttc cgacataaag
     2521 caggagattc agtagctcag ctggtagagc attacacttt taatgtagtg gtcctgggtt
     2581 cgaatcccag ctgaatcacc aaaatataac ccgatacttt tcttaagtat cgggttttct
     2641 atttcttaca gccagcctta gtgtgctttc tcccctattt gactaacttt gctgccttaa
     2701 ttagcaggag gtttcctgag catgaatgaa aatcgtcgcc tttcagcaaa agaaaaggct
     2761 cttaaaatta atcttgacac tcgcctgtat ggctcatttg ccgaaatagg tgccggacag
     2821 gaaactgcat cacacttttt tcgcgcgggc ggagcttctg gaacggttgc taaaactatg
     2881 tcggcttacg acatgcgttt ctccgatgcc atttacggaa aaacaaatcg ctatgtctgc
     2941 gaggaacgcc tccttcagat gctcaacaaa gaatacggat tacttggaaa acgtcttcct
     3001 tttcggaaag attcaaccaa ctttttcgca ttcgctaata ccgtcgaaac aactaatttc
     3061 caacgaacca atcaaggtca tggctggata ggtgttcgat tccaattaaa tccagcatct
     3121 gaacccaatg agtgtattat ccacattgtc ctaaaggata atgagaacac ctggcaacaa
     3181 cgcgctattg gtcaaattgg tgtaaacctg attttcggtt gcttttatca aactcaagaa
     3241 cccgacaatc tcgttaaatc acttctcgat ggtattattc cgggaacggt tgaagtagat
     3301 atgattcgct taaccggacc tgatttgcaa catatcgaca atcgcttact cagcttgaag
     3361 ttagttaagt atggactaac taaaactgct atgttgggcc ctgatggttc tgttttacaa
     3421 ccgtctgaag cgttgtacaa gaaaaatgct ttagtgatgt cgggacgatt tcgtccattt
     3481 accaaaactc acgaagacat gctcaaccga gcaacagccg actttgtgga agaacttgga
     3541 gatgaaaacc acctaatcgt tttgagtgag ctttcgctta atgcactcat tgatgccaat
     3601 ggtgatatta acgatgaaga tttcctggat cgagctaaca tgctttgttc actaggtcat
     3661 acggtattga tttctaactt cttagattac tggtatctag ttccctatct agctaaaaca
     3721 acccgaaatc aattggtagg atttgtactt agcgttcgaa acatggagcg aattttcgat
     3781 ccttcccaat acacgaattt aactggagga attctggagg cattaaccat gctttttggt
     3841 tcgaatgtaa aagcttatgc atatccagca tatcgtcgaa caacaggtga aattatcaca
     3901 cttgatagtc tccaaattgc acccgaatac caaggattac tacgctactt attagataac
     3961 aataagatcg aggatatccg aaatgccgac ttaagtgtat tacatattaa tgcggatgaa
     4021 gtattgaacc aaataaaatc agccgaacaa ggttgggaga aatttgttcc agtacgtgtt
     4081 gcagaaatca tcaaatcgaa cagcctgttt gactttcctg gtgattccga atcacaacta
     4141 attaataaaa actgaaaaat gggatttgaa acttttggct tttctcagga attactcgat
     4201 ggattagatg ccatgaattt cagggagcct actccggttc aatcccaggc cattcctgag
     4261 attatggcag gaaaggatct cattgccagt gctcaaacag gaacggggaa aactgcagcc
     4321 tttcttttac caataattga atcaaatctt ctaaatcaaa cgcatcatat cgataccctt
     4381 attatcgttc ctacacgtga attgggttta caaatcgatc aacaactaga aggctttgcc
     4441 tatttcgttc cggttagctc aattgcagtt tatggtgggg gtgatggaaa agaattcgaa
     4501 gttcaaaaac gtgctttgcg tgaaggaacc aac
//
LOCUS       DAOOQW010000198         4530 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_22768_length_4530_cov_5.592626, whole genome shotgun sequence.
ACCESSION   DAOOQW010000198 DAOOQW010000000
VERSION     DAOOQW010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4530
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22768_length_4530_cov_5.592626"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..497
                     /locus_tag="PK281_09780"
     CDS             <1..497
                     /locus_tag="PK281_09780"
                     /inference="COORDINATES: protein motif:HMM:NF015279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HOY49671.1"
                     /translation="HSMVDFFLLIAIAGAGDELQGIKRGIMEMADLVVVNKSDGDNLP
                     RAKAARQDIQRALHFFPELPSGWIPEAVLASSLESSGMEEIQDVISRFTQHVQTNGYF
                     AEHRKEQSRWWLKETIQHLLNELFFNQEEVKDKLVLTEQQVISQQVSVTEAARQLIQL
                     FRTK"
     gene            complement(500..1765)
                     /locus_tag="PK281_09785"
     CDS             complement(500..1765)
                     /locus_tag="PK281_09785"
                     /inference="COORDINATES: protein motif:HMM:NF019285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HOY49672.1"
                     /translation="MDKFHGLIGAIAILGIAFLFSNNRKAINYRLVFSGIALQSVIAI
                     LVLRIPPVTAFFQQLGHGMEKIEQFARQGASFAYGGIAVQQFDGTIGNYIGGGFVFAF
                     NVTATIILVCVLVAILYHVKIMQRVVSVIAKGMNFIMRVSGAEALSNVASAFVGQVEA
                     QVMIRPYLATMTKSELLASMSGSLACIAGGILVVYVNMGAAAGLDLAPKLIAASLMAA
                     PGALVISKILFPETEKSQTMGKVTLDVKSPYVNIIDAISHGASDGFKIAMNVIAMLIG
                     FIALVTMVDWILVHVAHLFDPNLDLSLNRIFSWLFYPLAWAMGVPSQDVGSVATLLGQ
                     KLTINEFVAFKSLASKSVPIVTEKGLLIVSIAICGFANFSSVGMQIGGIGELAPSRRA
                     DLAKLGMKALLAGTLASYLSATLAGIILG"
     gene            complement(1819..>4530)
                     /locus_tag="PK281_09790"
     CDS             complement(1819..>4530)
                     /locus_tag="PK281_09790"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOY49673.1"
                     /translation="DDEAITRAMIGFTELRTDEEKSYNIQEELFQSSTDLLKEQTALV
                     IRKLSHLSVADFLKIRKSLQESCQVAEAYFRLLGKKALEIMASAQVQETDFFQGKSGI
                     GVWFRKLANMEAGDDISPNSYVLKALIVQIWTAKKVDAQTLERIESIQDRLHDIGMEA
                     LESWKHQGPDYILNNILRDSIYSIALLNEISRVLDQIRDEQFIVHISEFNRRVADIVF
                     NEPAPFIFERLGEKYHHYLIDEFQDTSILQWQNLLPLIQNGLSENYPSLIVGDGKQAI
                     YRFRGGEVEQFARMPEPYPENLKDVQKERYTLLKGMFSPKTLDTNYRSKPEIVHFNNR
                     LYSFIQENQLPENFKNVYDDQEQLASPDKTDGYIQLEFVPADLEREDRDMHHLKRCEE
                     IIRDLIDNKKYRPRDIAILTRNNNQGTMLASHLLVAEIPVISSESLLVQNAPEVGFLI
                     SWMHILIGSNSSLNLLSVCNFLIHQHKLPYTTLESFLTQERIDEKSVLAFLKKNQFDL
                     ELSQLRKMSLLEACFRLCELFGISIQSNTYLQFFLEAVWNTGKSAALDIPLFLEYWEE
                     KKSKLSISLPQDANAVRVMTVHKSKGLQFPVVILPFAYGERPKTKNEWIDNEELLPEY
                     LSAARVKLTNTLKSTRLSELVTNEEERRALDAINLLYVATTRPEDALYILSGHSGIKG
                     SLLAGWETYLEQFASLEKPAGFAKGLCYWGDEHFENARNQESYTELSSVVHEYSAGKW
                     QKRITISRQAAKNWDVDQDKSALALGKLVHKALSYIETEDQTEEALTRLLQEGSLSIE
                     KASELKVLVESVTQHPQLNQLFKKGNRVFSERELLLPDGNIVIPDRVTERDGEITLID
                     YKTGLKRNEHQKQVSNYLKNLKELNYSSYKAYLVYLGKEIEIVEVLQ"
BASE COUNT         1343 a          931 c          885 g         1371 t
ORIGIN      
        1 tgcattcgat ggtcgatttc ttcttactga tagccattgc aggagcagga gatgaattac
       61 aaggcatcaa acgcggcatc atggaaatgg ccgatctggt tgtggtgaat aaatcagatg
      121 gtgataattt acctcgagca aaagcagcac gacaagatat tcaacgggca ttgcatttct
      181 ttcccgaatt accttctggc tggattcccg aagctgttct ggcttcttcg cttgaatcat
      241 caggcatgga agaaattcag gatgtaatta gtcgatttac ccagcatgtt cagacaaatg
      301 gatatttcgc agagcatcgc aaagagcaaa gtcgatggtg gcttaaagaa accattcaac
      361 atttattgaa cgagctgttt ttcaaccaag aagaggtgaa ggacaagcta gttctaacag
      421 agcagcaagt tatcagtcag caagtttctg ttacagaagc tgctcgacag ttgatacaac
      481 ttttcagaac aaagtaagat tagcctagaa tgatacctgc aagtgttgca gataagtagg
      541 aagctagagt tcctgctaga agggctttca ttcctaattt ggccaaatct gcacgacgag
      601 atggagctaa ttctccaatc ccaccgattt gcattcccac gctagagaag tttgcaaatc
      661 cgcaaatagc gatggataca atcaataatc ctttttcggt tacgataggt acagatttgc
      721 tagcaagact tttgaatgct acaaattcgt taatcgtaag tttttgtcca agtagggtag
      781 ctacacttcc tacatcctga gaaggaacac ccattgccca agcaagtgga tagaatagcc
      841 agctgaaaat tcggttaaga gaaagatcta gattcggatc gaatagatgt gcaacatgaa
      901 ctaaaatcca atccaccatg gtaaccaagg caatgaatcc aatcaacatg gcaatcacat
      961 tcatcgcgat tttaaaacca tcacttgcac catgtgaaat agcatcgatg atgttgacat
     1021 aaggactttt aacatccaaa gtcactttac ccattgtttg acttttttcc gtttcgggaa
     1081 acaaaatttt agagataacc aatgcacccg gtgcagccat caaactggca gcgattagtt
     1141 ttggagcaag atctaatccg gcagcagcgc ccatatttac gtaaaccacc aaaatacctc
     1201 cggcgataca agcaagactt ccgctcatac tggctaataa ttcactttta gtcatggtag
     1261 ccaggtaagg acgaatcatc acctgagctt caacttgtcc aacaaatgca cttgcaacgt
     1321 tacttaatgc ttcagctccg ctcacacgca tgataaaatt catcccttta gcaatcacag
     1381 aaacaacgcg ttgcatgatt ttcacgtggt acagaatagc tacaagcacg catacgagaa
     1441 taatggtagc tgttacgttg aaggcaaaga caaaccctcc accgatgtag tttccgattg
     1501 tgccatcgaa ttgttgcacg gcaattccac cataagcgaa ggatgctcct tgacgagcaa
     1561 actgctcaat tttctccata ccatgtccca attgttggaa gaatgcagtt actggaggaa
     1621 ttctcaaaac gagaatagca atgacgcttt gaagagctat tccgctaaaa accagacggt
     1681 aattaatggc cttgcggtta tttgaaaaaa ggaaggcgat acctaaaatg gctatagccc
     1741 cgattagtcc gtgaaattta tccataaaga caaagaaaag aaaatctgat tagcatcggt
     1801 agttttgcgt gaatcctttt attggagcac ttctacaatt tcaatttctt tccccaaata
     1861 cacgagataa gctttgtagg aagagtaatt gagttctttc aagtttttca agtaattgct
     1921 tacctgtttt tggtgttcgt tccgttttag tccggttttg taatcgatta gcgtgatttc
     1981 accatcacgt tctgttaccc gatcggggat aacaatattt ccatcgggaa gcaataattc
     2041 acgttcgcta aacacacgat ttcctttttt aaacaactga ttaagttggg gatgctgagt
     2101 aacagattcg actaaaactt ttaattcgga agctttttcg atggataaac ttccttcttg
     2161 taataatcgg gtaagtgctt cttcggtttg gtcttcggtc tcgatataag aaagtgcttt
     2221 gtgtactagt tttcctaatg ctaaagcaga tttgtcttga tctacatccc agttttttgc
     2281 agcttgtcga ctaattgtta ttcgtttctg ccatttacca gccgaatact catgtactac
     2341 gctggaaagt tcggtatatg attcttgatt gcgcgcattt tcaaaatgct catcacccca
     2401 ataacacaaa cctttcgcaa aaccagcggg tttttccaac gaagcaaatt gttcgagata
     2461 cgtttcccaa ccggctagta gtgaaccttt aattccacta tgtcccgata aaatatacaa
     2521 agcatcttcg gggcgtgtag tagctacgta aagcaaatta atagcatcca aagcacgacg
     2581 ttcttcttca ttggttacca attcagataa acgtgtggac ttaagcgtat ttgttaattt
     2641 cacgcgagct gcactcaaat attcaggtag aagttcctca ttgtcaatcc actcattttt
     2701 agtcttaggt ctttctccgt aggcgaatgg taaaataaca actggaaatt ggagcccttt
     2761 ggatttatgt acagtcatta ctcgaacggc atttgcatct tgaggtaatg aaatacttag
     2821 cttcgacttt ttctcttccc aatattctaa gaacagagga atatcaagcg ctgcagactt
     2881 tccggtgttc caaactgctt cgaggaagaa ttgtaagtag gtgttgcttt gaatgctaat
     2941 tccaaacagt tcgcataaac gaaaacatgc ttccaagagc gacattttcc tgagttgaga
     3001 caattctaaa tcgaattggt ttttcttcag gaatgctaaa acactctttt catctatgcg
     3061 ctcctgagtc aaaaatgact caagtgtagt gtagggaagt ttatgctgat gaattagaaa
     3121 attgcaaaca gatagcaggt tgagtgagct gtttgacccg attagaatat gcatccaaga
     3181 aattaggaaa ccaacttcgg gagcattttg aaccaataag gattcacttg aaataactgg
     3241 tatttcagca actaagagat gactcgctag cattgtcccc tgattgttat tccgtgttaa
     3301 aatggcaata tcacgtggac gatatttttt gttgtcgatt aaatcccgaa taatttcctc
     3361 acaacgtttg agatgatgca tatcccggtc ttctcgttct aaatcggcag gaacgaattc
     3421 taattggata tagccatcgg tcttatcggg agatgctaat tgttcctgat catcgtaaac
     3481 atttttgaag ttttcgggta gctgattttc ttgaataaac gaatatagcc ggttgttaaa
     3541 atggacaatt tcgggtttgc ttcggtagtt tgtgtccagt gtttttggac tgaacatccc
     3601 ttttagcaag gtgtatcgtt ctttctgaac atcttttagg ttttcgggat acggttcagg
     3661 catgcgagca aattgctcta cttcaccacc tcgaaatcga tatatcgctt gtttcccatc
     3721 accaacaatt aaggaaggat aattttccga taatccattt tgaattagtg gtaataaatt
     3781 ctgccactga agtattgaag tatcctgaaa ttcatcaatc aaataatgat ggtatttctc
     3841 ccccaaacgt tcgaaaataa agggcgctgg ctcgttgaaa acgatatctg caacacgtcg
     3901 gttgaattcg gaaatatgaa cgataaattg ttcatcccga atctgatcta gaacgcgact
     3961 aatctcattt aatagagcaa tggaataaat cgaatcgcgt agaatgttgt ttaagatgta
     4021 atcaggccct tgatgtttcc aactttctaa cgcttccatt ccaatatcgt gaagcctgtc
     4081 ttggatagat tcaattcttt ctaaggtttg tgcatcaact ttcttagcag tccagatttg
     4141 aactataaga gcttttaaga catagctatt tgggctgata tcatcgcctg cttccatgtt
     4201 agcaagcttt ctaaaccaaa ctccaattcc acttttgccc tgaaaaaaat cggtttcttg
     4261 aacctgtgct gatgccatta tttcgagcgc cttctttcct aataatcgaa aataggcttc
     4321 tgcaacctga caagattcct gaagtgattt acgaatcttt aaaaagtcgg caacacttaa
     4381 atgacttagt tttctgatta ctaaagctgt ttgttctttg agcaaatcgg tgctcgattg
     4441 gaacaattct tcttgaatgt tatagctctt ttcctcatcc gttcttaact cagtgaaacc
     4501 aatcatggcg cgagttatcg cttcatcatc
//
LOCUS       DAOOQW010000199         4526 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_22792_length_4526_cov_5.328562, whole genome shotgun sequence.
ACCESSION   DAOOQW010000199 DAOOQW010000000
VERSION     DAOOQW010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4526
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22792_length_4526_cov_5.328562"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /locus_tag="PK281_09795"
     CDS             complement(<1..713)
                     /locus_tag="PK281_09795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49674.1"
                     /translation="MQKRHIWFWFIFSTMLTACGGSSEPSVKALRVEESKAELPDVPV
                     RFQRFEQDLFRFSKESFTQDTLKMYQKYGSFFDLYTSQVIRIGNKHLPLFRENLLGFI
                     QDPDIRSVKAETDRLFSNTDTIKSEISTAFNRYHKAFPDSVIPAVSTLISGFNYNVVV
                     SDSNLAIGLDMYLGKNCKFYELLALPKYKVSKMNRTMIVPDAIRGWLMSNFEMDASQE
                     DLVSFMIYQGKMMYLSQQL"
     gene            812..1522
                     /locus_tag="PK281_09800"
     CDS             812..1522
                     /locus_tag="PK281_09800"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HOY49675.1"
                     /translation="MKRLFLITLLFAISATTMNAQRSFRFGMKAAPLFSWIKPDFRDA
                     PKDFAVEGGGGRIGFVWGPMAEIQLNETFLISTGLDINYTSGKLKGTLKDSLGNLVDW
                     NQLYKTRFIELPVMLKFRTKEIGYLRYFGLFGMGAGFRYSAKTEFSRTLNSTTVTNND
                     NNSDTYVNVFRGSMLVGAGVEYSLSGNTAMVGSLLFNNSLTNMFKNQTGDYITDPFNT
                     IRENGIMNYMQLNIGILF"
     gene            1575..3227
                     /locus_tag="PK281_09805"
     CDS             1575..3227
                     /locus_tag="PK281_09805"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF010588.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HOY49676.1"
                     /translation="MKIALAQINTHIGNFDTNVALIIQNIERAKRDGADLVVFPELSV
                     CGYPPLDFLEFDDFIQRCDLAVSKIAEACQDMAAIVGCPTRNPQLEGKNLFNSAIFIE
                     NGRVKQFVHKTLLPNYDVFDEYRYFEPNTSFNCVEFKNKRIALTICEDLWDIEEDKMY
                     VRWPMEELKIQQPDLIINIAASPFSYKHAESRKRVLHQTASKYKIPVFYVNTTGAQTE
                     LIFDGGSMVLNRDGNVVCELNYFQDDFRSFELDWVNTAKCVPYQANQFNSPELEAGRI
                     HQALILGIQDYFRKMGFKQAVLGLSGGVDSALVYTLAVQALGKDNVLGILLPSPYSSD
                     HSISDSLELAARLGARTEKLPIGDVFQQFQNTLASAFKGLPENLTEENLQARIRGVLL
                     MAMSNKFGYILLNTSNKSEAAVGYGTLYGDMCGGIAVIGDVYKTQVYELCRYINREEE
                     IIPINILTKAPSAELRPGQKDSDSLPDYDILDKVLFKYIEERKGPEELVQLGFEKALV
                     DRVLKLVNTNEYKRFQTPPILRISPKAFGMGRRMPLVGKYLS"
     gene            complement(3224..>4526)
                     /locus_tag="PK281_09810"
     CDS             complement(3224..>4526)
                     /locus_tag="PK281_09810"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOY49677.1"
                     /translation="DPFVRYNGKKGWSHVIRNRWFHLNNNNDNNQSNLSDVLYSEYQI
                     QRQFKDGFLSGLSLTSGILHSYTIGSSDLYKGGLSDTITEKAQSGNRNIAAYLQLNRT
                     FFTRLTLSAGFRYEHFSINNPTVSASDSTKTTKEGKPVFRAGANLRIHKATFVRASFG
                     QGYRFPTIAEKFIRTQVGPIRIYPNDSLKSETSWNAEIGVKQGFRINKFQGYFDAAYF
                     IQKFYNNIEFNFGAFGQLSDPLFGLGFASLNIGNTQVTGVDFSLAGQGNIRKTGIAML
                     IGYTHTNPISLEPNKAYPVIDISTKNVTYARSSSDSTGYLLKYRFQHVFKADMEITRG
                     KWVCGASVRYNSYMQNIDKIFEDLDEILGALQQPKPGLKAYRQAHNKGTTVVDMRLSY
                     QLNKTSKVAFVVNNLFNMEYTIRPMSVEQPRTTAIQFSLAF"
BASE COUNT         1354 a          941 c          888 g         1343 t
ORIGIN      
        1 aatagttgct gacttaaata catcattttg ccctgataaa tcataaacga aacgagatct
       61 tcttgcgagg catccatctc aaaattgctc atcaaccaac cacgaatggc atcaggaaca
      121 atcatggttc gattcatttt cgaaacttta tacttcggaa gtgccagcaa ttcataaaac
      181 ttgcaattct ttcctaaata catgtcaaga ccaatggcta ggttcgaatc cgaaaccact
      241 acattataat taaaaccaga aatgagcgtt gatacagcag gaattacact atcgggaaat
      301 gctttatggt agcgattgaa tgcggtgcta atctcagatt ttatcgtgtc ggtattcgag
      361 aataatcggt cggtttcggc ttttacggaa cggatgtcag gatcttggat aaatcctaat
      421 aggttttcac gaaataaagg aaggtgcttg tttccaattc gtataacctg actggtgtat
      481 aagtcgaaaa acgaaccgta tttctgatac atcttcagcg tgtcttgcgt gaagctttct
      541 ttcgagaacc gaaatagatc ttgctcaaac cgttgaaaac gcacaggaac gtcgggcaat
      601 tcagctttcg actcctctac tcgtagcgct tttaccgatg gttcggatga tccgccacag
      661 gcagtaagca ttgttgaaaa aatgaaccaa aaccaaatgt gtcgtttctg catgtgtccg
      721 aatcacgtta tctttgctga caacggccaa aattagtaaa tcgtaccgtg aattgcggtt
      781 ttccttaagt acccatcaac cttttatttt tatgaaacga ctttttctga ttacgctgct
      841 ctttgcaatt tctgcaacta ctatgaatgc tcagcgtagt tttcgctttg ggatgaaagc
      901 cgctccatta tttagctgga taaaacccga ttttcgtgat gctccaaaag actttgctgt
      961 agaaggcggt ggaggaagaa ttggctttgt ttgggggcca atggccgaaa tccaacttaa
     1021 tgaaactttc ctaatttcta ccggtttaga cattaattac acttcgggaa aactaaaagg
     1081 aacattaaaa gattctcttg gcaacctggt agattggaat caactttata aaacgcgctt
     1141 catcgaactt cctgttatgt tgaagttcag gaccaaagaa attggatact tacgctattt
     1201 cggcttattt gggatggggg ctggttttcg ttattctgca aaaacagaat tctctcgcac
     1261 cttaaattct acaactgtta caaacaatga caacaactcc gatacatacg taaatgtatt
     1321 tagaggttcg atgcttgtag gtgctggagt agaatattct ctttcaggaa atactgcaat
     1381 ggtgggcagc ctactattta ataactcgct cacaaacatg tttaaaaatc aaacgggtga
     1441 ctacattact gatcctttca acaccattcg cgaaaacggt atcatgaact atatgcaact
     1501 taacatcggc atcctgttct gatttacaat acttctacct ttgcagccgg atctcaaaat
     1561 ggtccggctt ttttatgaag attgccctag cacaaataaa cacgcatatc gggaatttcg
     1621 atacgaacgt tgcgctcatc attcaaaaca ttgaacgagc aaaacgcgac ggtgccgact
     1681 tagtcgtatt tcccgaacta agcgtttgtg gatatccacc tctcgacttc cttgagtttg
     1741 acgactttat tcaacgctgc gatctggcgg tgagtaaaat tgcggaagcg tgtcaagaca
     1801 tggctgcaat tgttggctgt ccaacccgaa acccacaact agaaggaaaa aacttgttta
     1861 attctgccat cttcatcgaa aacggtcgag ttaaacaatt cgttcataaa acgcttttgc
     1921 ctaattacga tgtgtttgat gagtaccggt actttgaacc aaacaccagt ttcaattgcg
     1981 tagaattcaa aaacaagcgc attgctttaa caatctgcga agacctttgg gacatcgaag
     2041 aagataaaat gtatgtgcgt tggccgatgg aagaactcaa aatccagcag cctgatttaa
     2101 tcatcaacat tgccgcttca cccttttcat acaaacacgc agaaagccgg aaacgcgtat
     2161 tgcatcaaac tgcatctaag tataaaatcc cggtatttta tgtcaatact acgggagcgc
     2221 aaactgaatt gattttcgat ggtggatcaa tggttctaaa tcgagacgga aatgtggtat
     2281 gtgaactcaa ttatttccaa gatgatttta gaagttttga gctcgattgg gttaacaccg
     2341 ccaagtgcgt tccttatcaa gccaatcaat tcaattcacc agagttagaa gccggccgaa
     2401 ttcaccaagc attaattctc ggaatccaag actacttccg aaaaatgggc ttcaaacaag
     2461 ctgtattagg acttagcggc ggtgttgatt ctgccttggt ttacacgctt gctgttcaag
     2521 ctttaggaaa agataatgta ttggggattt tacttccttc tccatattct tccgaccact
     2581 caataagcga ttcactagaa ttagccgctc gccttggagc acgtaccgaa aaacttccca
     2641 ttggagatgt ctttcaacag tttcaaaaca cgcttgcttc agcatttaag gggcttcccg
     2701 agaatttaac agaggaaaat ttacaagctc gaatccgcgg agttctctta atggctatga
     2761 gtaataagtt tggatacatc ttgctcaata cgtccaataa atcggaagct gcggttggtt
     2821 atggcactct atacggtgat atgtgcggag gaattgccgt gattggtgat gtgtacaaaa
     2881 ctcaggtgta cgaactctgt cgatacatca atcgagagga ggaaattatt cctatcaaca
     2941 tcctcacaaa agccccaagt gctgaattaa gacccggaca aaaagatagc gactcactac
     3001 ccgattatga tatccttgat aaagttcttt tcaagtatat cgaagaacga aaaggtccgg
     3061 aagagttggt ccaacttggt ttcgagaagg ccttggtcga ccgagtatta aagttggtca
     3121 acaccaatga atacaagcgt ttccaaactc ctccaatctt acggatttca cctaaagctt
     3181 ttggaatggg ccgaagaatg ccattggttg gtaaatacct ttcttaaaaa gcaagactaa
     3241 actgaatcgc tgttgttcga ggttgctcaa cactcattgg tcgaatggta tattccatat
     3301 tgaacagatt gttgactacg aatgccacct tgcttgtttt atttagctga tagcttaatc
     3361 gcatatcaac gacagttgtt cctttattat gtgcctgacg ataagccttt aagccgggtt
     3421 taggttgttg aagtgcgccc aatatttcat ccagatcttc aaaaatctta tcgatgtttt
     3481 gcatgtaact gttatatcga acactcgctc cacacaccca ttttcctcgg gtaatttcca
     3541 tgtcggcttt gaaaacatgc tgaaaacgat atttcaataa atatcctgtt gagtcgctgg
     3601 aagaacgagc ataagtaaca ttcttcgtag aaatatcaat caccggataa gccttgttcg
     3661 gctctaatga aattggatta gtatgcgtgt agccgatcaa cattgctata ccagttttgc
     3721 gaatgtttcc ttgtccagca agagaaaaat caactcccgt aacctgagtg ttaccaatgt
     3781 tcaaggatgc gaaacctaat ccgaataaag gatctgaaag ttgtccaaat gcgccaaaat
     3841 tgaattctat gttgttataa aacttctgta tgaaataagc agcatcaaaa tagccttgga
     3901 acttgttaat tctaaatcct tgcttaacac caatttcagc attccaagat gtttcagatt
     3961 ttaaactgtc gttcggatag atacgaattg gtccaacttg agtgcggata aatttctctg
     4021 caattgttgg aaaacgatat ccttgtccaa atgaagcccg aacaaatgtg gctttgtgta
     4081 tccgcaagtt cgctcctgct ctaaaaaccg gttttccttc tttagttgtt ttggtagaat
     4141 cggatgctga tacagtcggg ttatttatag aaaaatgctc atatcggaag cccgcactta
     4201 atgtcaaacg agtaaaaaag gttctgttca attgtaaata cgctgcaata tttctgttgc
     4261 ccgattgagc tttctctgta attgtgtcgg ataatccacc cttatacaaa tcagatgatc
     4321 caatagtata ggaatgtagt attccagagg tcaaactcag tccgcttaaa aaaccgtctt
     4381 taaactgacg ttgaatctga tattccgaat acaacacatc gcttaaatta ctctgattgt
     4441 tatcgttgtt gttattcaag tgaaaccacc tattccgaat aacatggctc catcctttct
     4501 ttccattgta tcgaacaaat ggatca
//
LOCUS       DAOOQW010000200         4512 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_22892_length_4512_cov_4.084810, whole genome shotgun sequence.
ACCESSION   DAOOQW010000200 DAOOQW010000000
VERSION     DAOOQW010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4512
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22892_length_4512_cov_4.084810"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..729)
                     /locus_tag="PK281_09815"
     CDS             complement(<1..729)
                     /locus_tag="PK281_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009421236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOY49678.1"
                     /translation="MSDIIKHECGIVLIRLLKPLDYYRKKYGSARYALNKLHLLMEKQ
                     HNRGQDGAGIACIKFDVEPGERYIGRFRSNAPQALREIVEKVNSYFEEIRINEPSKLN
                     DTDYLKDQVPFIGELLMGHLRYGTFGKNSIESCHPFLRQNNWRSRNLVMAGNFNMTNV
                     EELFNVLVDLGQHPKDKVDTVTVMEKIGHFLDEEVQGLFIKYKDQFTNKEVSDIIETE
                     LNLQNVLRRACKDFDGGYTMAGMIG"
     gene            1009..1254
                     /locus_tag="PK281_09820"
     CDS             1009..1254
                     /locus_tag="PK281_09820"
                     /inference="COORDINATES: protein motif:HMM:NF025088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HOY49679.1"
                     /translation="MPTILYILGWRLFFYSNEGNEPIHVHAEKAEMECKYWLLVDKFE
                     IEEANSFNLKPSSRREIRKIIFEHFDLIVDSWNKHFN"
     gene            1265..1495
                     /locus_tag="PK281_09825"
     CDS             1265..1495
                     /locus_tag="PK281_09825"
                     /inference="COORDINATES: protein motif:HMM:NF021873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HOY49680.1"
                     /translation="MVHIRKIEGIHFIGDSLCLTVDDKFYQIELNEISKKLAQADNMK
                     RMMFVLSPSGYGIHWPLLDEDISIEGLIKMKS"
     gene            1634..3880
                     /locus_tag="PK281_09830"
     CDS             1634..3880
                     /locus_tag="PK281_09830"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOY49681.1"
                     /translation="MKKSYTHVSFRAFYLLLVVFLAGVGRSVAQNDTPCDAISVPVSS
                     GTCVFTPGTSVGGTYQNNAANGGFPNCGFSGSADVWYSFVVPPSGAVAVTLEPGTIND
                     AAMSLYSGPCTNPVLIGCDDDSGLDFMPAIDRSDFTPGDVIYIRVWNNNSPTGTFGVC
                     VTESHSDCGTASILCSNLAVTSNAYGGGSNQDGDWSGVNCIPDEYQSQWFKFQVINPC
                     TFSFTLTPDAIAPGMYPDYDWTLWEVTGTPNVDFCDSFLPDIPAVACNASSSMGPNGE
                     TGVGPSGVNASEPAGPGNPYSASFSVSAGDIFYLWVNNFTNSSSGFNFQFGGDVVLNC
                     DLDTPDSTITTTSTSTCPGTCNGSATTTVANMTAPYTYSWSANAGGQNTANIHNLCAG
                     TYIVNVTDALGVVIADTVEVENTPAIAFTTGFIDNTSCHSPNGKAWVNASGGTGSLVY
                     AWNSSAVTSDTLSGLSAGSYTVTISDGNTCSVDTSIIIHTQVDYPTIESIARTDSTAC
                     GALCTNFSVIFQGGNQYFWDFGDGENSTSNHPYHCFTAPGDYDVSLIVSTDGGCSDTI
                     TAPNFIRVNANPTAHFYSVLSDPFHASFHDQTNDAVRWLWQMGDSLNTQSIEQNPEFD
                     YSAFQEYCIQLTVWDANNCMDDIHQCIEFKPELSVYVPTAFTPNGDGLNDVFYIEGYP
                     MKVMEFWVYNRWGEQLFYTQDPTSGWDGRRFQNGEISPQDLYLYKTSVKGERGEIREL
                     TGCFQLVH"
     gene            3944..>4512
                     /locus_tag="PK281_09835"
     CDS             3944..>4512
                     /locus_tag="PK281_09835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49682.1"
                     /translation="MLKYLRFLVLGLAFIPQAQAQFTSLYSVPSGSFLQDVVFADTNT
                     MMVTGGNLFRTSIDGGRNWIGLSSGGISVTAAHFPTKQFGITVGTQGLYRINDECGYF
                     WGWGNNKSIGVTKDLLDVYFINNQKGIVCGNDGTLRLTSNQGTNWTAIATGTTNHIKG
                     VWMVNDSTSFACANGGIILKVINQTVSSS"
BASE COUNT         1258 a          819 c          935 g         1500 t
ORIGIN      
        1 tccgatcata cctgccatgg tgtatccacc atcaaaatct ttgcatgctc tgcgaagtac
       61 gttttgcaaa ttcagctcag tttcaatgat gtcactgact tctttgttgg tgaactgatc
      121 cttatattta ataaataagc cttgaacttc ttcatccaaa aagtggccga tcttttccat
      181 aacggtcacg gtgtcgactt tgtctttcgg atgttgaccg aggtcgacta acacattgaa
      241 tagctcctcc acattcgtca tgttaaagtt tccggccatc accaagttgc ggctgcgcca
      301 gttgttttga cgaagaaaag ggtgacaact ttcgatgcta tttttcccga atgtcccata
      361 gcgtaaatgt cccatgagga gttcgccaat gaaaggaact tgatctttga ggtagtcggt
      421 atcgttgagt ttgctaggtt cgttaatgcg aatttcttcg aagtaagaat tcaccttttc
      481 cacaatttcg cgaagagcct gaggagcatt cgagcgaaat cgaccaatgt agcgttctcc
      541 cggttcgaca tcgaatttga tacacgctat accagcaccg tcttgaccac gattatgctg
      601 cttttccatc agcaaatgca gcttatttag cgcatatcgg gcagaaccgt atttcttgcg
      661 gtaataatca agaggtttga gcaggcggat gagaacaatt ccacactcat gtttgattat
      721 atcgctcata ggattgtccg gttgggatgg cttattccgg cgctgcaaag atagtcagtc
      781 tagatagaat gaagaaagga tatttataag caaattctaa aaaaatactc aattgtaaat
      841 tacatttcta cttctctatt ggaaagaatt tttttttgat actcttctaa atgcttgaaa
      901 tcaatataag tttgccgttt ttatttatca atatagatta agttacattg accattgggg
      961 ctattttata tctgtttata ttcttgtttt tacttatatt tgataataat gcctacaata
     1021 ctctatatac taggttggcg actctttttt tattcaaatg agggaaatga acctattcat
     1081 gttcatgcag aaaaagccga aatggaatgt aaatattggt tgttggttga taagtttgaa
     1141 attgaagaag caaattcatt taacttgaaa ccgtcatcaa gaagagaaat tcggaaaatc
     1201 atttttgagc attttgattt aattgttgat tcttggaata aacacttcaa ttaaaattag
     1261 acttatggtt catattcgta aaatagaggg tattcatttt ataggtgact cattatgtct
     1321 caccgtggat gataaatttt atcaaattga attaaatgaa atatctaaaa aattagctca
     1381 agctgataat atgaaacgaa tgatgtttgt attatctcct tcgggttatg gtatacattg
     1441 gcctttgtta gatgaggata tatctattga gggattaata aagatgaaaa gttaacttaa
     1501 tgtattaagt tctttgcagt taaatgtttg cttaggcctt tttctttgtt aaaaacagtt
     1561 gttagtattt cattttctgc ttgaatatga atattttact atttttagaa tccattcaga
     1621 gccactatca ttgatgaaaa aaagctacac gcatgtttct tttcgtgcct tctacttgct
     1681 gctagtggtt tttctggcag gagttggaag aagtgtagct caaaatgaca ctccctgcga
     1741 tgctatttcg gtgcctgtaa gttcgggaac ctgtgttttt acacccggca cttctgttgg
     1801 cggcacgtat caaaataatg cggctaatgg aggttttccg aattgtggtt tttcaggatc
     1861 ggcggatgtt tggtattcgt ttgtagttcc accttcagga gctgttgctg taaccttaga
     1921 gcctggaacc atcaatgatg ctgccatgtc tttgtattct ggaccatgta ccaaccctgt
     1981 tttaattggt tgcgacgatg atagcggatt agattttatg cctgccattg accgttctga
     2041 tttcaccccg ggtgatgtga tatacattcg tgtttggaat aataattcac ctaccggaac
     2101 atttggtgta tgtgtaaccg aatcgcattc tgattgcgga actgcttcaa ttttatgctc
     2161 caatttagct gttacttcaa atgcatatgg aggaggctcg aatcaagatg gagattggtc
     2221 aggagtgaat tgtattccgg atgaatatca atctcaatgg ttcaagtttc aagtcataaa
     2281 cccttgtaca ttcagtttta cactaactcc cgatgctatt gctcccggta tgtatcccga
     2341 ttatgactgg actttatggg aagtaacagg aactcctaac gtagattttt gcgatagctt
     2401 tttgccggat attcctgctg ttgcctgtaa tgcatccagt tcgatgggac caaatggaga
     2461 aacaggtgtt ggtcctagtg gcgtgaatgc aagtgagcca gctggtccgg gaaatccgta
     2521 tagtgcttca ttttccgttt cggcgggtga tatattttat ctctgggtaa ataatttcac
     2581 gaattcatcc tctggattta actttcaatt tggaggtgat gtagtattga attgcgattt
     2641 agatactccc gattcaacta ttacaacaac gagcacttct acttgtcctg gaacatgcaa
     2701 cggttctgca acaacaactg tagcaaacat gactgcaccc tatacatatt catggagcgc
     2761 taatgctggc ggacaaaaca ccgctaacat ccataattta tgtgcaggta cttatatcgt
     2821 aaatgtaact gatgctttgg gagttgtaat tgctgatact gttgaggttg aaaatacccc
     2881 tgcaattgct tttacaactg gatttataga taataccagt tgtcattcac ccaatggtaa
     2941 agcatgggta aatgcgagtg gtggaacggg tagtctagtt tatgcttgga attcttccgc
     3001 tgtaacttcg gataccttgt caggactaag tgctggttca tatacggtaa caattagcga
     3061 tggtaatact tgttcggttg atacttctat tatcatacat actcaagttg actatccaac
     3121 aattgaatca attgctcgca ccgattctac tgcttgtgga gcattgtgta ctaacttctc
     3181 ggtaattttt cagggaggaa atcaatattt ctgggatttt ggtgatggag aaaacagtac
     3241 atctaatcat ccatatcact gttttactgc tccaggcgat tatgatgtga gtttgattgt
     3301 atcgactgat ggtggttgta gcgatacgat aactgctccc aattttattc gggtgaatgc
     3361 taatccaaca gcacatttct attccgtttt atcggatcca tttcatgcat ctttccatga
     3421 tcaaacaaat gatgcagttc gttggttatg gcaaatggga gatagtttga acacccaatc
     3481 cattgagcaa aacccagaat ttgattactc tgctttccaa gaatactgta ttcaattaac
     3541 cgtttgggat gcgaataatt gcatggatga tattcatcaa tgtattgaat tcaaacccga
     3601 attatcggtt tatgttccaa cggcattcac acctaatgga gatggtttga atgatgtttt
     3661 ttacatcgaa gggtatccca tgaaagtgat ggagttttgg gtgtataatc gatggggaga
     3721 acaacttttt tacacccaag accctacatc aggttgggat ggaagaagat ttcagaacgg
     3781 tgaaatttcc cctcaggatt tatacttgta taaaacatct gtgaaaggag aacgcggtga
     3841 aattcgtgaa ttaaccggct gttttcaatt agttcactag gctgaaccat tttccaaaaa
     3901 catcataagg ggagctttgt caatcattcc ctttctttgt accatgttaa aatatctacg
     3961 tttcctagtt ctaggcttag cttttattcc ccaagctcaa gcacaattta ccagtctgta
     4021 tagcgtgcca tctggttctt ttttgcaaga tgttgttttt gccgatacca atactatgat
     4081 ggttactggc ggaaatctat ttcgaacctc aattgatggt ggtcgaaact ggattggttt
     4141 atcctcaggt ggtatttctg ttactgccgc gcattttcct actaagcaat ttggaattac
     4201 agttggtact cagggacttt atcgtatcaa cgacgaatgt ggttatttct ggggatgggg
     4261 aaacaacaaa tctattggcg taactaagga tttattggat gtttatttca tcaacaacca
     4321 aaaaggaatt gtatgtggaa acgatggaac attaaggttg accagcaatc aaggaactaa
     4381 ctggacagcc atagccaccg gtacaacgaa tcacataaaa ggcgtttgga tggtgaatga
     4441 ttctacttcg tttgcttgtg ctaatggagg aataattctg aaagtcatca accaaacggt
     4501 gagttcgagt tt
//
LOCUS       DAOOQW010000201         4489 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_23018_length_4489_cov_4.281461, whole genome shotgun sequence.
ACCESSION   DAOOQW010000201 DAOOQW010000000
VERSION     DAOOQW010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4489
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23018_length_4489_cov_4.281461"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2274
                     /locus_tag="PK281_09840"
     CDS             <1..2274
                     /locus_tag="PK281_09840"
                     /inference="COORDINATES: protein motif:HMM:NF036531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HOY49683.1"
                     /translation="KLSVGIKEKSGKEMEYTLALVDDGLLDLTRFKTPDPWSSFYARE
                     ALGVVSWDLYDDVIDAYSGAFESVVTIGGDAELDGKKKSTKANRFKPMVRFIGPFKLK
                     KGGSANHSISIPAYFGSARIMVVAGNPQQAYGSAEKRISIRKPLMALATLPRVVSPGE
                     TVRMPISIFALEKSVQQISISCTPNPLFTLTSPSRQTISMKSPGERLVEFEFKVANTT
                     GLAKVKVVVSSGKETAIVETELDVRTPNLPQTDVTEVILKPGQVWQNTLEVKGMAGTR
                     EASVELSGMPAINLSKRLKELTEYPHGCVEQTTSAAFPQLFLQNLVDLSEVDKKRISN
                     HVKAAIKRLALFQTSTGGMAYWPGGSQADDWSTCYAGHFLIEAQTAGYPVSSSMMKKW
                     KNYQKATSRLWKSTYTQAGFDQIQAYRLYTLALAGSPEIGAMNRMREVKSISPQARWH
                     LASAYQLAGQKETARQLLKGLSQNFSSSYYTTFGSVHRDEALALLTLSEMGMVSESYR
                     LARTVAENLGRNEWMSTQTTAWSLLALSKNNPAPKSGKRILAELTIGKKSQKISGNKP
                     LVEVPLQLDASGKSALMIKNTNQVPLYARIIRRSTFGSDQLVSANQNQLQLQVSFTDM
                     TGKDLDPSAIPQGTDFTAHLTIRNTSSTNGFNNLALSMLFASGWEINNTRVNDVANVQ
                     EGSSYEYQDIRDDRVLTYFQLLPGTSKTYSFKFNAAYVGRFYLPAILCEAMYDSTVFA
                     RSASRWVQVVRPQPKAI"
     gene            2382..>2650
                     /locus_tag="PK281_09845"
     CDS             2382..>2650
                     /locus_tag="PK281_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018942658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein beta subunit/FixA
                     family protein"
                     /protein_id="HOY49684.1"
                     /translation="MKILVCISQVPDTTSKIAFTDNNTKFSEDKVQYIVNPYDEWYAL
                     VRALELKESLGGNVTIITVGTAAVEPVIRKALAIGADDAVRIDADP"
     assembly_gap    2651..2750
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2751..2870
                     /locus_tag="PK281_09850"
     CDS             <2751..2870
                     /locus_tag="PK281_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007647977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein beta subunit/FixA
                     family protein"
                     /protein_id="HOY49685.1"
                     /translation="PLVSVKEFSLPAPKSAVKLIDPSNMDQLVELLHSEAKVI"
     gene            2873..3853
                     /locus_tag="PK281_09855"
     CDS             2873..3853
                     /locus_tag="PK281_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013550921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HOY49686.1"
                     /translation="MAVLIVTETSKGKFNKASLEAISYGAQVARQLSTTAAVLSIGAG
                     SDDELAKAGEYGVSSVLKADSAKLAHFDAQLVSKLVLAAMEKSGADVLIMSHDATGKA
                     VAPRVSAILQAGLVSGVIALPNTSGGFTVRKNVFSGKAFAEYSISTAKKIITLLPNSV
                     PVQATGGTATVTAFNEVADAFQSVLKVEERIHQTDGEIPLPGAELVVSAGRGMKGPEN
                     WGMIEDLAKTLGAATACSRPVADIGWRPHHEHVGQTGLAIRPNLYIAVGISGAIQHLA
                     GVNGSKVIVVINKDSEAPFFKAADYGVVGDAFEIVPKLNEAFRKFKAQNA"
     gene            3906..>4489
                     /locus_tag="PK281_09860"
     CDS             3906..>4489
                     /locus_tag="PK281_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HOY49687.1"
                     /translation="MKKIKLEISGLSYSQTQSGAYALVLGENGGKRRLPIIIGGFEAQ
                     AIAIELEKMAPTRPLTHDLFKSFAESFHVQVREVVIYNLVEGIFYAKLICQQDGANDV
                     EVDARTSDAIALAVRFNCPIYTYEFILSSAGILIDDADDDSDPALPPAESSSSPQPVA
                     KEPGSELSGKSKDELKQMLKAAIEDEAYEKAGKIR"
BASE COUNT         1299 a          902 c          960 g         1228 t
ORIGIN      
        1 aaactatcag ttggtatcaa ggaaaagagc ggtaaggaaa tggaatatac cttggctttg
       61 gtggatgatg gattattgga tttaactcgt ttcaaaactc ccgatccatg gtcatctttt
      121 tatgcaagag aagcacttgg cgtagtttct tgggatttgt acgatgatgt gattgatgcg
      181 tattctggag ctttcgaaag tgttgttacc attggtggag atgctgaact agacggaaaa
      241 aagaaaagca cgaaagccaa ccgattcaaa ccaatggttc gttttattgg accttttaaa
      301 cttaagaaag gcggttccgc taatcattca atcagtattc cggcatattt tggatcagct
      361 agaatcatgg ttgttgccgg taatcctcaa caagcttatg gaagtgccga aaaacgcatc
      421 agcattcgga aaccgctcat ggctttagca actttgccgc gagtggttag tccgggtgaa
      481 acggttcgta tgccaattag catttttgcc ttagaaaaat cggttcagca aataagcatt
      541 agttgtacgc ccaatccttt gtttacgctt acaagtcctt ctcgacaaac gattagcatg
      601 aaatctccgg gcgagcgttt ggtagaattt gaattcaaag ttgccaatac taccggttta
      661 gctaaagtga aagtcgttgt tagcagtggc aaggaaacag ctatcgttga aacggaatta
      721 gatgtccgca ctcctaacct accacaaaca gatgtaacgg aagtgattct aaagcccgga
      781 caagtgtggc aaaatacact ggaagtcaag ggaatggcag gaactcgaga agcatcagtt
      841 gaactttcgg gtatgccggc aatcaatcta agtaagcgct taaaagagct gactgaatat
      901 ccacatggat gcgtggaaca gactacttct gctgcttttc ctcagttatt ccttcaaaat
      961 ttagtggatt taagtgaagt tgataaaaaa cgaatcagca accacgtaaa agctgccatt
     1021 aaacgactcg ctttgtttca aacttcaacg ggcggaatgg catactggcc gggcggttca
     1081 caagctgatg attggtcaac ttgctatgca ggacattttc taatcgaagc tcaaactgca
     1141 ggttatccag ttagttcttc catgatgaag aaatggaaaa actaccaaaa agctacttct
     1201 cgtttatgga aaagcaccta cacacaagct ggtttcgatc aaatacaagc ttatcgttta
     1261 tatacgctag ctcttgccgg aagtcctgaa attggtgcga tgaatcgtat gcgggaagtg
     1321 aagtctatta gtccgcaggc acgttggcat ttagcatctg cttatcagct ggctggacaa
     1381 aaagaaacag cccgacaact tttaaaaggt ttatcacaaa acttcagttc gagttattac
     1441 accacgtttg gtagcgtaca tagggatgaa gcattggcat tattgacttt gtcggaaatg
     1501 ggaatggttt cggaatcata tcgtttggct cgaacggttg ccgaaaatct aggtagaaat
     1561 gagtggatga gcacacaaac taccgcttgg tcattacttg ctttgtcgaa gaataaccct
     1621 gcacccaaat cagggaaacg aattttagct gaattaacga ttggtaagaa atctcagaaa
     1681 ataagtggta acaagccttt ggtagaagtt cccttacaat tggatgcatc cggaaaatct
     1741 gctttgatga ttaaaaacac taatcaagtt cccctgtatg cgcgcataat tcgtcgaagt
     1801 acgtttggtt cggatcaatt ggtgagcgct aatcaaaatc agcttcaatt gcaggtgagt
     1861 tttaccgata tgactggaaa agatttagat ccttcagcca tccctcaagg caccgatttc
     1921 acagcacatt taaccattcg gaacacatct tctaccaatg gattcaacaa tctggctttg
     1981 tcgatgttat ttgcatccgg ttgggaaatt aacaatacac gtgtgaatga tgttgccaat
     2041 gtacaggaag gttcgagtta tgagtatcaa gacattcgag atgatcgcgt gttgacttat
     2101 ttccaactac ttccgggcac aagtaaaaca tattcgttca aattcaacgc cgcgtatgta
     2161 ggacgtttct atttaccagc aattttatgt gaagcgatgt acgattcaac tgtttttgca
     2221 cgatcagctt cacgctgggt tcaagttgtt agaccacaac ccaaagctat ttaagtgatt
     2281 tagaagctat ttgattttat ctcttaaagg ctatttcgaa ttactcagat tgaattttca
     2341 ctacatttgc acgcccaaaa acacacatca ataccgacga aatgaagata ctcgtttgca
     2401 tcagtcaggt acccgatacc actagcaaaa tcgcatttac cgataacaac accaagttca
     2461 gtgaagataa ggtgcaatac attgtcaacc cgtatgacga atggtatgcc ttagttcgcg
     2521 ccttggaatt aaaagaaagc ctaggaggca acgtaacgat catcacagtt ggaacagctg
     2581 ctgttgagcc tgttatcaga aaagctttag ccattggtgc tgatgatgcg gttcgaattg
     2641 atgctgatcc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cctttagtta
     2761 gtgtgaaaga attttcatta cctgctccta aatcagccgt taagctgatc gatccttcca
     2821 atatggacca acttgttgaa ctccttcatt ctgaagcaaa agttatctaa tcatggcagt
     2881 acttattgtt accgaaacct caaaaggtaa attcaataaa gcttcacttg aagccatttc
     2941 atacggagca caagttgccc gtcaactttc tacaacagct gctgttcttt cgattggcgc
     3001 tggttccgat gatgaattag caaaagctgg tgaatatggt gttagctctg tattaaaagc
     3061 agatagcgct aaactcgcac atttcgatgc tcagttagtt agcaagctag ttcttgctgc
     3121 tatggagaaa tccggagctg atgtcttaat tatgagtcac gacgctacag gtaaagctgt
     3181 tgctcctcgc gtttctgcaa tccttcaagc tggattagtt tcgggagtga tcgcacttcc
     3241 caatacttct ggtggattca ctgttcgtaa aaacgttttt tcaggaaaag catttgccga
     3301 atattccatc agcacagcaa agaaaatcat caccttattg ccaaactccg ttcctgttca
     3361 agctactgga ggaacagcaa cagtaactgc ttttaacgaa gtagctgatg catttcaatc
     3421 ggtattaaag gttgaagaac gtatccacca aacggatggc gaaattccac ttccgggtgc
     3481 tgaactagtt gtttctgccg gtcgcggtat gaaaggacct gaaaactggg gaatgattga
     3541 agatttagct aaaactttag gtgctgcaac tgcatgttct cgtcctgttg ctgatatcgg
     3601 atggcgtccg caccacgaac atgtggggca aacaggacta gctattcgtc cgaatctata
     3661 cattgctgtt ggaatttctg gagcaatcca gcatttggcc ggtgttaatg gttcgaaagt
     3721 tatcgttgta atcaacaaag attcggaagc accattcttt aaagcggccg attatggtgt
     3781 agttggtgat gcttttgaaa ttgttcctaa attgaacgaa gcttttcgta aattcaaggc
     3841 tcaaaacgcc tgatcagaat ctaattctgt cgaccgaaag tctagtactt tcttgtgaag
     3901 cccttatgaa gaaaataaag ctcgaaatat ccggactatc ctacagtcaa actcaaagtg
     3961 gagcctatgc gctagttttg ggtgaaaacg gaggaaaacg tcgccttccg ataatcatcg
     4021 gcggatttga agcacaagca attgccattg agctcgaaaa aatggctcct acccgaccgc
     4081 ttacacacga tttatttaag tcgtttgccg agtcgtttca cgttcaggtt cgagaagtgg
     4141 ttatctataa tttggtggaa ggaatattct acgccaaact tatctgccaa caagacggag
     4201 ccaatgatgt agaggttgat gctcgaactt cagatgccat tgcattagct gttcgattca
     4261 actgcccgat atacacgtac gaatttatcc tcagctctgc cggtattctt atcgacgatg
     4321 ctgatgatga tagcgatcca gctttacctc ctgccgaaag ctcttcaagt cctcaacctg
     4381 ttgccaaaga accaggtagc gaactcagcg gtaaaagcaa agacgaatta aagcaaatgc
     4441 tcaaagccgc catcgaagat gaagcttatg aaaaagctgg taagattcg
//
LOCUS       DAOOQW010000202         4425 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_23459_length_4425_cov_3.627918, whole genome shotgun sequence.
ACCESSION   DAOOQW010000202 DAOOQW010000000
VERSION     DAOOQW010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23459_length_4425_cov_3.627918"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2226
                     /locus_tag="PK281_09865"
     CDS             <1..2226
                     /locus_tag="PK281_09865"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOY49688.1"
                     /translation="VYIPAIKAGAVTNAYGFYSITLPKGTYEVQYSFVGYKTQVVSIV
                     LNANVARNLELASESTLMEEIVVSDRAIDENVIGLQMSKNVIDMAQIKKLPALFGEVD
                     IIKSVQMMPGVIYAGEGTSAFYVRGGSADQNLILIDEAPIYDPSHLLGLFSVFNADVI
                     KDSELYRGGIPARFGGRLSSILEVRTKDGNNKNYDVTGGIGTLASRLAVEGPIVKDKS
                     SFIVSGRRSYADVFLAAANQENTVFFYDVNAKVSWKFSNKDRVFAAFYAGRDKFSFGD
                     DFGFDWGNKTGTLRWNHLFNDRLFSNTTFIINSFDYKLELRDPVQGFSWTSNLQEISL
                     KNDLSYFINTNNDLTFGYHITGRKFSPGKISPTTSTSIFAPVELDQMYAIDHALYLSN
                     LQRLNDKITFDYGVRLSIFQNVGSSDVILYSDPQDNAEITRTDTVHYDAWENIKTYVN
                     LEPRLSMRYILGKGQSLKFSYNRMAQNTHLIAAGTVPLPFNTWSPSGYYLEPQLADQF
                     AIGYFRNSKNNMYEFSTEVYYKDMQHVTDFADNASIFFNEDLSTEFRQGKAVSYGIEF
                     MATKTKGKLTGMVNYTLSKAERTIEGVNLGRTFDANYDRRNVVNVQAAYELSKRWTFG
                     GTFTYSTGRPITLPTGKFEYQNYKPSVVTERNGYRLPDFHRLDLSATLTPKKNENRKW
                     KGSWVFSVYNVYNRKNPLTIYTRTAQDEDGNVIGDGTKKEARMIYLFPIFPSVTYNFK
                     F"
     gene            2233..3066
                     /locus_tag="PK281_09870"
     CDS             2233..3066
                     /locus_tag="PK281_09870"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 domain-containing protein"
                     /protein_id="HOY49689.1"
                     /translation="MKNILLIIVSIALLTACEKTIDLDLEQSESKIVIEGLVTNHTGY
                     QYVRVTRTAGFYDDGATPRVTNANVSVKDDTGNEFLFIHNPGNEEDSAGYYLPDVSFN
                     GEIGRTYTLTVEVDGEIYTAQDKLFATSGIDSLGYQINPDEEEDPKEDTKFYEALLYM
                     TEPQDEENYYLFKFFRNDSLKVYNDNDIYFADDKILGETIEGLGSPVYYALNDTARIE
                     MYSISRAGYIYYLDLQSLMNNDGGLFSQPPSNSRNNLSNGALGFFQASAVDVKTILIE
                     E"
     gene            3286..4341
                     /locus_tag="PK281_09875"
     CDS             3286..4341
                     /locus_tag="PK281_09875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49690.1"
                     /translation="MFRKLNPIFLKRIVIIFCFACVSLSGATDVFAQDDKDISIPLEK
                     FYVDRNGIGLFRKIISKVTIGISTGYGSTKFKHDLSGFGILQNPDSLPRIFAGTNSGN
                     GNTNWFNTSTYSTATSQPGSSQIDANTTKLGFKSKAFNIPIKATLHVEFDRYRIGGGY
                     SIEYTHIGTFKPTAHGDQINNYTPEVSSLWLKKYFGMVGGTVYRYYEYSLVVDAQVGG
                     YGLGNSFDKSLIKKGVFINLGATVERDFSEYFRVFVRPSYELKSYKISLPELGQSVTH
                     KFNAFYINIGASYRFPELRRCVVKTCKAQMNHAHGNREYRSRVHPIYKKQNPHYGENH
                     PVLIKYKGKNKRKIDPY"
BASE COUNT         1352 a          834 c          926 g         1313 t
ORIGIN      
        1 gtttacattc ccgccatcaa ggccggagcc gttaccaatg cttatggatt ttactccatc
       61 accctcccga aaggaacgta tgaagtgcaa tacagctttg taggctacaa aactcaagtt
      121 gtttcgattg tactaaacgc caatgtagcg cgcaaccttg aattggcgag tgagtcaacg
      181 ctcatggaag aaatcgttgt gtccgatcgg gctatcgatg agaacgttat tgggctccaa
      241 atgagcaaga atgtgattga catggcgcaa atcaaaaaac ttcctgcctt gtttggtgaa
      301 gtggacatta ttaagagtgt tcagatgatg cccggagtaa tttatgcagg agaaggtacc
      361 tctgcatttt atgttcgggg tgggagtgcc gatcaaaatt taattctcat tgacgaggcg
      421 cctatttacg atccatcaca tttacttgga ttgttttcgg tattcaatgc cgatgtgatc
      481 aaggactcgg aattatatcg tggcggaata cccgcccgtt ttggtggaag gttatcatct
      541 attttggaag tgcgcaccaa ggatggaaat aataaaaatt atgatgtcac cggtggtata
      601 gggacactcg cttctcgcct cgcggtagaa ggaccaattg taaaagataa gagttccttt
      661 atcgtttcag gcagaagatc gtatgcagat gtatttttag cagcagccaa tcaggaaaat
      721 accgttttct tttatgatgt aaatgcaaaa gtaagttgga agttcagtaa taaagaccgt
      781 gtgtttgctg cgttttatgc tggccgcgat aaattttcct ttggcgatga ctttggtttt
      841 gattggggaa ataaaacagg aacacttcgg tggaatcatt tatttaatga tcggttattt
      901 tccaacacaa cattcattat aaacagcttt gactataaat tggaattaag agatcctgtt
      961 caaggttttt cctggacttc taacttgcaa gagatcagtc tcaagaatga tctttcgtac
     1021 tttatcaaca ccaataatga tttgactttt ggatatcaca tcaccggaag gaaattttct
     1081 cccggaaaga tatcaccaac aacatctacc tctatttttg ctccggtcga gcttgatcag
     1141 atgtatgcca ttgatcatgc actttaccta agcaatctac agcggttaaa tgataagatt
     1201 acctttgatt atggagttcg gttatccatt ttccaaaacg ttggtagcag cgatgttatt
     1261 ctgtattcag atcctcagga caatgcagag attacacgta cggacacggt gcattatgat
     1321 gcatgggaaa acattaagac gtatgtaaac ctggagccac gtctctccat gcgttacatt
     1381 ttaggaaaag gtcaatctct taagttctcg tataaccgaa tggcacaaaa tactcacttg
     1441 attgcagcgg gtactgtacc cttgcccttt aatacctgga gtccgagtgg atattactta
     1501 gaacctcagc tggccgatca gtttgctatt ggctatttca gaaactcaaa aaataatatg
     1561 tatgagttct ccaccgaagt atattacaaa gacatgcagc atgttacaga ctttgccgat
     1621 aatgcttcca ttttctttaa cgaagatttg tcaaccgaat ttcgtcaggg caaagctgtt
     1681 tcatatggta tagagtttat ggctacaaaa acgaagggga aactcacggg tatggtcaac
     1741 tataccttat caaaagcaga gcgaaccatt gagggtgtaa accttgggcg aacatttgat
     1801 gcaaactacg acagaagaaa cgtagtgaac gtacaggcag cgtatgaact cagtaaacgt
     1861 tggacatttg gaggaacctt tacgtatagt acaggcagac caattacatt accaaccggg
     1921 aaatttgaat atcaaaacta taaaccgtct gtggttacgg agcgaaatgg atatcgttta
     1981 cccgattttc accgattgga tttgtcggct accttaacac caaagaaaaa tgaaaatcga
     2041 aagtggaagg gttcctgggt cttctcggtg tacaatgttt acaatcgaaa aaacccgctt
     2101 actatttata ctcgaacagc gcaggatgaa gatggaaatg ttattggtga tggcacaaaa
     2161 aaagaggcac ggatgattta cctcttcccg atattccctt ctgttaccta taactttaaa
     2221 ttttaattga tcatgaaaaa tatactttta ataatagtat ccattgccct tctgacagct
     2281 tgcgaaaaaa caatcgatct ggatttagag caatccgaat caaagattgt aatcgaaggt
     2341 ctggttacaa atcatacagg ttatcaatat gtgcgggtaa cacgaacagc cggattttat
     2401 gatgatggag caactccacg ggttaccaat gccaacgtat ccgttaaaga tgatacaggt
     2461 aatgagtttt tattcattca taatcctgga aatgaagaag attctgccgg atattattta
     2521 cctgatgtaa gcttcaatgg cgaaatcgga agaacctata cacttacagt tgaagtagat
     2581 ggagaaatct ataccgccca ggataagctg tttgcgacta gtggaatcga ttcactaggt
     2641 tatcagataa atcctgatga agaggaggat ccaaaagagg ataccaagtt ttacgaagca
     2701 ttactataca tgacggagcc acaagatgaa gagaactatt acctgtttaa attctttcgc
     2761 aacgattctc tgaaagtata caacgataat gatatttatt tcgctgatga taaaatttta
     2821 ggtgaaacga ttgaaggctt aggctctcct gtgtattatg ctctaaacga tacggcacgc
     2881 atcgagatgt acagcatttc acgcgctggt tacatctact atttggattt acaaagcctg
     2941 atgaacaatg atggtggttt gttcagtcag ccgccttcaa actcacgtaa caatctttcg
     3001 aacggagcat tgggtttttt ccaggctagc gctgtcgatg taaaaacaat cttgattgaa
     3061 gagtaattca agtattcatc cattcgcttc gcacgaagct tgtaggcgaa tggatgaagt
     3121 tactttacgt tagaggtcaa cgatcggcaa ttttgtttcc tgattacctt acatactttc
     3181 atcccgggtc tttcgttgaa aattcgtatg tcttacgaag taggatgatt acacaaattg
     3241 gtttaatttt aggcattaaa ggccattcta actggcttat attttttgtt tagaaagctt
     3301 aaccctattt ttctgaagcg tattgtcatc attttttgtt ttgcctgtgt tagcctgtct
     3361 ggtgctacgg atgtattcgc tcaggatgat aaagacatct ccattccact agaaaaattc
     3421 tatgttgatc ggaatggtat aggccttttt cgtaagatca tcagtaaagt aacaataggt
     3481 atcagtacag gctatggaag cacaaaattt aagcacgact taagcggttt tggtatactg
     3541 caaaacccgg attctttgcc ccgcattttt gcaggaacta attcaggaaa cggcaacacc
     3601 aattggttca atacttctac gtattctaca gctacttcac agccaggaag ttctcaaatt
     3661 gatgctaata caaccaaact tggtttcaag agcaaggctt ttaatatccc catcaaggca
     3721 acacttcacg ttgaatttga tcgctatcga ataggaggag ggtactccat tgaatacact
     3781 cacatcggga catttaaacc cactgctcat ggtgatcaaa tcaacaatta tactcctgaa
     3841 gtttcaagtc tatggctgaa gaaatacttt ggtatggttg gcggaacggt gtatcggtat
     3901 tatgaatatt cattggtggt tgatgcacag gttggggggt atggtttggg caatagtttt
     3961 gacaagtctt taattaaaaa gggggtcttc attaatttag gagcaactgt tgagcgtgat
     4021 ttttctgagt actttcgtgt cttcgtacgt ccatcgtatg aacttaaaag ctataaaatt
     4081 tccttacccg agcttggaca aagtgtaacg cataagttca atgcatttta cattaacata
     4141 ggtgcgagct atcgttttcc agaactgaga agatgcgtag tgaagacttg taaagctcag
     4201 atgaatcatg ctcatggtaa tcgtgaatac agaagtagag tacacccaat ctacaagaaa
     4261 cagaatccac actatgggga aaatcatcct gtcctaatca aatacaaagg aaaaaataag
     4321 agaaaaattg acccgtatta gagcaagaat tacgggttaa tagtcggcag ttatttcgcc
     4381 agaatttcat ccttttccat tcacaattaa ccattaacaa ttaac
//
LOCUS       DAOOQW010000203         4418 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_23504_length_4418_cov_4.060509, whole genome shotgun sequence.
ACCESSION   DAOOQW010000203 DAOOQW010000000
VERSION     DAOOQW010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4418
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23504_length_4418_cov_4.060509"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="PK281_09880"
     CDS             <1..225
                     /locus_tag="PK281_09880"
                     /inference="COORDINATES: protein motif:HMM:NF016162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HOY49691.1"
                     /translation="EGKQYFPHTAVKPNYFIESERHSVCPIKGTANYYHVEVNGHRQS
                     DAAHYFPNPNPRVKVIQNYISFNDDIDIEE"
     gene            complement(228..845)
                     /locus_tag="PK281_09885"
     CDS             complement(228..845)
                     /locus_tag="PK281_09885"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOY49692.1"
                     /translation="MLIRIHPVTPDPRQLKKVVEILKNGGVIIYPTDGVYAFGCDIQK
                     SRAVERVAQLKGIKPEKADFSFVFDELRHITEYTRPVDTPVYKLLKSCLPGPYTFILN
                     ANNSVPKLFKNNKRTVGIRIPDNAICRAIVTELGSPIISSSVHDEDDIIEYTTDPELI
                     HERYKDLVDAVVDGGYGALVPSTIVDCTGNVPELIRQGAGEVDFE"
     gene            complement(845..1501)
                     /gene="fsa"
                     /locus_tag="PK281_09890"
     CDS             complement(845..1501)
                     /gene="fsa"
                     /locus_tag="PK281_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019038078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HOY49693.1"
                     /translation="MKFFIDTANLDQIRDAQDLGVLDGVTTNPSLMAKEGIQGADRIL
                     KHYVDICEIVDGDVSAEVISTDYEGIIREGENLASLHPQIVVKVPMIRDGVKAIKYFT
                     KMGIRTNCTLIFSAGQALLAAKAGATYVSPFIGRLDDISTSGVGLIEQIRLIYDNYGY
                     ETEILAASIRHTMHLIECAEAGADVATCPLNVITDLLKHPLTDIGLEKFLADYKKTNG
                     "
     gene            1645..1986
                     /locus_tag="PK281_09895"
     CDS             1645..1986
                     /locus_tag="PK281_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor"
                     /protein_id="HOY49694.1"
                     /translation="MAKPKDRVNVVYSTNPNFQYETDQELEQDTLPANQQNLKISLDK
                     KARAGKQVTLIAGFIGKSDDLESLAKLLKNLCGSGGSSKDGEILIQGDHRDKILNYLI
                     SKSYKAKKIGG"
     gene            complement(1983..3116)
                     /gene="bshA"
                     /locus_tag="PK281_09900"
     CDS             complement(1983..3116)
                     /gene="bshA"
                     /locus_tag="PK281_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020081855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HOY49695.1"
                     /translation="MKIGIVCYPTFGGSGVVATELGMALAAQGHQVHFISYSQPFRLD
                     VFSGNLFYHEVPVTNYPLFEFLPYEISLTSKLVDVVMHQELDLLHVHYAIPHASAAYM
                     ARQILQKRGKRIPFITTLHGTDITLMGRDPSYQPVIEFAINESDGVTAVSDSLRQDTL
                     KYFDISREIEVIPNFIDLENYAKPSDLCFKGMYAPNGERILTHISNFRPVKRIDDVVR
                     VFERVHREVPSKLILAGDGPERGNVENLARQLGVIQDVVFLGKTKAIERILCMSDLFL
                     LTSETESFGLSSLEAMASYVPVISTNTGGIPEVNVHGYSGFLSNVGDVDDMAKNALKL
                     LKDEALMEQFKGQARKQAAKFNITEILPMYERMYSRLTGLPLN"
     gene            complement(3129..3557)
                     /locus_tag="PK281_09905"
     CDS             complement(3129..3557)
                     /locus_tag="PK281_09905"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF025034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HOY49696.1"
                     /translation="MNIALPIEIVDLRDDGMHLFITAKAGRKNCRLLLDTGASRTVFD
                     STQFQKLLPNAKLVDLESLSTGLGTDSLPGKVSSIAHFKLGDFKVIDFPVIVLDLNHV
                     NRSYESLGFKSIHGVLGSDILFKFKAEIDYGKLELRLHVN"
     gene            complement(3613..>4418)
                     /gene="mdh"
                     /locus_tag="PK281_09910"
     CDS             complement(3613..>4418)
                     /gene="mdh"
                     /locus_tag="PK281_09910"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012841129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HOY49697.1"
                     /translation="TAPINLYDSRIVGYTNDYSKTANSEVVVITSGLPRKPGMSRDDL
                     IATNAGIVKSVTENVIAHSPDAKIIVVSNPLDVMTYVAYLTAKVDARRVFGMAGILDT
                     ARYRAFLAEALHVSPKDIQAVLMGGHGDTMVPLPRYTTVSGIPVTELIDAARLDAIID
                     RTKKGGGELVNLMGTSAWYAPGAAAAQMVEAIVRDQKRIFPCCALLNGEYGLSNIYLG
                     VPVKLGKNGIEEIIELKLNAEETKLLNESAKAVKEVMDVYDAMAKSATV"
BASE COUNT         1414 a          973 c          745 g         1286 t
ORIGIN      
        1 gaaggcaaac agtattttcc acatactgcc gtaaaaccca attattttat tgaaagtgaa
       61 cgacattcgg tttgtcctat taaaggaaca gccaattatt atcatgtcga agtgaatggc
      121 caccgtcagt cagacgcagc tcattatttc cccaatccca atccaagagt taaggttatt
      181 caaaactata tcagctttaa cgacgatatt gatatcgaag aatagactta ttcgaaatca
      241 acttctcctg caccctgacg aataagttca ggcacatttc cggtacaatc tacaatagta
      301 gaaggaacca atgcaccata accaccatca accacggcat caaccaggtc tttataacgc
      361 tcatggatta gttccggatc ggtcgtatat tctatgatat catcctcatc atgaaccgag
      421 ctggatataa ttggacttcc cagttcagtt acaatagctc gacaaattgc attatcagga
      481 atacgaatac ctactgttcg tttgttgttc ttgaacaatt ttggaacact gttattcgcg
      541 ttcaaaatga aagtatacgg tccgggcaaa caactcttca atagcttata aaccggagta
      601 tctacaggac gagtatattc ggtaatatgc cttagctcat cgaaaacaaa agagaaatct
      661 gctttttcgg gttttatccc ttttaattga gcaacacgct caactgccct tgatttctga
      721 atatcacatc caaaagcata cacaccatct gttgggtaga taattacccc accattctta
      781 agaatttcaa caaccttttt taattgccga ggatcgggtg taaccggatg tattcgaata
      841 agcattaacc gtttgttttc ttgtaatcag cgagaaattt ctctagccca atatcagtaa
      901 gcggatgctt tagtaaatcg gtaattacat tcaatggaca agttgctaca tcggctccag
      961 cttctgcaca ttcaatcaaa tgcatggtgt gacgaataga agcagctaat atctccgttt
     1021 cgtagccgta gttgtcgtaa atcaaacgaa tctgctcaat taaaccaaca ccactagtag
     1081 aaatatcatc caaacgacca ataaaaggtg acacatatgt tgctcctgct ttagctgcta
     1141 ataatgcttg tccggcagaa aagatcaagg tacaattggt acgaattccc attttcgtga
     1201 aatacttgat agcctttaca ccatcacgaa tcataggaac tttaacaaca atttggggat
     1261 gaagtgaagc taaattttcg ccttcgcgga tgataccttc gtaatcggtt gaaatcactt
     1321 cagcactcac atcaccatca acaatttcac atatatcgac atagtgtttt aggatacgat
     1381 cagcaccttg aattccctct ttagccatca agctaggatt ggtagttact ccgtctaaaa
     1441 cgccaaggtc ttgtgcatca cggatctgat ctaaattagc tgtgtcgatg aaaaatttca
     1501 ttgttgtatg gttgtttgaa gcggcaaaaa tacgttaaat gaatggcatt taagtacaaa
     1561 gaaattgtcg atttatatgc agatgagctg ttcatgttag aacattaact ggagttctat
     1621 atcttcacaa cgatttctaa agtcatggct aaaccgaaag atcgagtgaa cgtggtttac
     1681 tcaaccaacc ccaattttca atacgaaact gatcaggaac ttgaacaaga taccctacca
     1741 gctaatcagc aaaacctaaa aatttcgctc gataaaaaag ctcgtgctgg taagcaagtt
     1801 actttgattg caggatttat tggcaaatcg gatgatctgg aatccttagc taaactcctt
     1861 aagaacttat gtggttctgg gggaagttct aaagatggag aaatattaat ccaaggtgat
     1921 catcgagata aaatcctgaa ttaccttatt tcaaaatcat acaaagccaa gaaaatcgga
     1981 ggctagttca aaggcaaccc cgttaatcgc gagtacatcc gttcatacat cggaagtatt
     2041 tcggtaatgt taaatttagc tgcttgtttg cgtgcttgcc ccttaaactg ttccattaaa
     2101 gcttcatcct tcaataattt caaggcattc tttgccatat catctacatc acccacattg
     2161 cttagaaagc ccgaatatcc atgtacatta acttctggaa tacctcctgt attggttgaa
     2221 ataacaggaa cataactcgc cattgcttcc aaagatgata atccgaagct ttcagtctca
     2281 gaagtcaaaa ggaataaatc agacatacat aatatccgtt cgattgcttt agtcttccct
     2341 aagaacacca catcttgaat cacacctaat tgtcgagcta gattctctac attcccacgt
     2401 tctggcccat cacccgccaa aatgagcttt gatggaactt cacgatgcac gcgctcaaat
     2461 actcgaacaa catcatcaat tcgcttcact ggtctgaaat tcgaaatatg tgtcagaatc
     2521 cgttccccat tgggtgcata cattcctttg aaacataaat cggaaggttt tgcataattc
     2581 tccaaatcaa taaaattggg aatgacttct atctcccgac taatatcgaa gtacttcaac
     2641 gtatcctggc ggagtgaatc cgaaacagct gtcaccccat cactttcatt tatagcaaat
     2701 tcaattactg gttgatagga tggatcgcgc cccatcaagg tgatatcggt tccatgcagc
     2761 gttgtgataa atggaattcg cttcccacgc ttttgcaata tctgacgagc catataagct
     2821 gcagaagcat gaggaatggc ataatgtaca tgcaaaagat ctaactcttg atgcattaca
     2881 acatccacta atttggacgt caatgaaatc tcataaggaa gaaattcaaa taagggataa
     2941 ttcgtaacgg gtacttcgtg gtaaaataaa ttccccgaaa acacatccaa cctaaatggc
     3001 tgactatacg aaataaaatg tacctgatgt ccttgagctg ctagagccat cccaagttca
     3061 gttgccacta ctccacttcc accaaaagtt ggataacaaa caattccaat tttcatcttc
     3121 ctgcaaattt agttgacatg taacctcaat tccaatttcc cataatctat ctctgcttta
     3181 aacttaaaca ggatatcact tcccaaaact ccatgaattg atttaaaacc aagtgattcg
     3241 tatgatcgat ttacatgatt caaatccagc actataactg gaaaatcaat taccttaaaa
     3301 tcacccaatt taaaatgtgc aatagaactc actttacccg gcaaagaatc agttcctagt
     3361 ccagttgata agctttctaa atcaaccaat ttagcatttg gcagtagctt ttggaattga
     3421 gttgaatcaa aaacggttct cgaagctcct gtatcaagta aaagtcgaca attttttctg
     3481 cctgctttag ctgttatgaa aagatgcatt ccatcatctc gtaaatcaac tatctcaatg
     3541 ggcaaagcta tattcatggg ccggaataaa aaaggggccc gtgagagccc cttccattag
     3601 aagaattaaa tcttaaacag tagccgattt agccattgca tcataaacat ccattacttc
     3661 tttcacagct tttgcgcttt cattaagaag tttggtttct tctgcattca atttcaattc
     3721 aataatttct tcaataccgt tctttcctaa tttaacagga acacctaagt aaatattact
     3781 caagccatat tcaccattaa gtaaagcaca gcatgggaaa attcgttttt gatcgcgtac
     3841 aattgcttct accatttgag cagcagcagc acctggtgca taccaagccg aagttcccat
     3901 tagatttact aattctcccc cacctttctt agtacggtcg ataattgcat ctaaacgtgc
     3961 tgcatcaatt aactcagtaa ctggaattcc tgatacagtt gtgtaacgtg gaagtggaac
     4021 catagtatca ccatgaccac ccatcaatac tgcttgaata tcttttggag aaacatgaag
     4081 tgcttcagct aaaaatgcac ggtaacgagc agtatctaaa atacctgcca taccaaatac
     4141 acgacgagca tcaacttttg cagtcaaata tgctacataa gtcattacat ctaatggatt
     4201 cgaaaccaca ataatcttag catctggcga atgtgcaata acattctccg ttaccgactt
     4261 cacgattcct gcattggttg caattaaatc atcccggctc attcctggtt tacgcggtaa
     4321 tcctgatgtg attacaacaa cttcagaatt agctgttttt gaataatcgt tggtataacc
     4381 cacaatacga gagtcataca gattaatcgg agcagttt
//
LOCUS       DAOOQW010000204         4401 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_23608_length_4401_cov_5.308790, whole genome shotgun sequence.
ACCESSION   DAOOQW010000204 DAOOQW010000000
VERSION     DAOOQW010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4401
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23608_length_4401_cov_5.308790"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /locus_tag="PK281_09915"
     CDS             <1..682
                     /locus_tag="PK281_09915"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOY49698.1"
                     /translation="VRAVLLDVNETNTIDTVVQQAWAFFQGVDVVVNNAGYGLTGAVE
                     ETTEAEIRAQMETNFFGALFVTKAFLPKFRLQGHGTFVQISSQAGISSTPGLGIYNAS
                     KFALEGFSEALSKEAVHLGIRVMLVEPGPFRTDWAGNSMVFSPVELAEYASSAGVMRR
                     RLSQISGNQPGDPIKGVQLIIRAVDSEKMPLRLALGNIAVDTISAKLKQVQADIETWE
                     QESRATDF"
     gene            737..4216
                     /locus_tag="PK281_09920"
     CDS             737..4216
                     /locus_tag="PK281_09920"
                     /inference="COORDINATES: protein motif:HMM:NF027232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY49699.1"
                     /translation="MLNRIFCFALFLVLGNTIQGQTFDPPILSNDPTIPEWAAKMYAP
                     SPNVLEVDRLFRAYYATHPFKQNNYTRYYKRWRRYVTPYLNDDGTIHYPDATERQAMA
                     DASRSVQVQRSNTWSFLGPIVHRRGKYAVTDENVQMSYHANVYCIDRSMSNPQVLYCG
                     TECGGVYKTVDQGVNWEYVTENNIIEDVSAISVDPLNENTVIFSAANEFWRSIDGGAN
                     WTIVGNTAFQNLNIKAYQFNFHPTNPQIVFAATNLGLYKSTDNGINWSQIFSGESMSV
                     EFKPGNSQVVYALQFNANTGISDFYKSTDGGTVFTIKPNGWFSVPAADEGLIENRGGR
                     IAVTQANPERVYVLLVGESNAAANLQLRGTIGIYSSANAGESWSFPHGQQGMPYDVNT
                     HPNLMDFDGYSSDYDQIYYNTALVCSQLDETKLLIGGLNLWRSNDGGAAYQTVGGYIG
                     NLPNMHVDLQEFKVYKTSANSEEFWFSSDGGINLSNDWCTTHESRTRGIYASNFWGMD
                     QGWNDDIIVGGRYHNGNAAYSPTFDTGLFLSIGGGESATGYVNYSPERKTYFSDIGGR
                     KIPLLQDDVIGTFSTNLYPNESYYDNASSRMLFDWNYWNIAYMGRDNVLYKSTDGGAS
                     FGPLYTFGTDATAKVYWIEQSHANPLVMYVQQVVSNVSKLWKTTDGGITFSQVALPQS
                     KRELYFTLSHTNADELWLAFTTGSNGNKVYTSTNGGTLWTNITSSALNGLSIKSIAHQ
                     AGTNGGVYVATFHNAVYYRSNTNTTWTVVGTGLPASIYPLRLMPFYQKNKLRLGSWQL
                     GVWEIDLVEPSAVIADFSSDFSSFTCPGDTLWFVNHSVCDSSATFSWSFPGATPSTST
                     SLAPKVTYAAAGTYPVTLYVTQNGQTFSITKDAFITTQTSASIPLAENFESGTLPSEW
                     KFYDDGNDGANWIISDLASSFGQGTYNMFFDNYYIDVAGKRDRFLTRKLDFSSAAAVQ
                     LSFDIAYSQYDNNYSDSLAVLVSTDCGITYSSVYLKGGDALATAPDLTSERFEPINSQ
                     WRRDTVIIWDAAGASDVIIAFENRGRYGQPIYVDKINIQTLSSVEIPIVPNVQVDVFP
                     NPVSSSLTFRLKGYSGKQADAEIIDVNGKRCKEFRVLGSQMELSVNDLAAGIYFLRVK
                     TNSGMMQKRFVKQ"
     gene            complement(4217..>4401)
                     /locus_tag="PK281_09925"
     CDS             complement(4217..>4401)
                     /locus_tag="PK281_09925"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HOY49700.1"
                     /translation="FAYGLQSSQSTINVSGSGDAEITVSDHLDVTISGSGNVHYKGQP
                     TINTSISGSGKLHHEN"
BASE COUNT         1255 a          798 c         1014 g         1334 t
ORIGIN      
        1 agttcgagct gttttgttgg atgtgaacga aacaaacaca attgatacag ttgttcaaca
       61 ggcttgggcg ttttttcaag gtgttgatgt ggttgtaaac aatgccggat atggtttaac
      121 tggagcggtt gaggaaacta ccgaagcaga aattcgggct caaatggaaa cgaacttttt
      181 cggggcttta tttgtgacaa aagcatttct acccaagttt cgacttcaag gtcatggtac
      241 tttcgtgcaa atatcatcgc aagctggaat cagcagtaca cccggtttag gaatttataa
      301 tgccagtaaa ttcgccttag aaggtttttc tgaagcattg tcgaaagaag cggtccattt
      361 aggaattcgt gttatgttgg ttgaacctgg accgtttaga accgattggg ctggaaattc
      421 aatggttttc tctcctgttg aattagctga atatgcatca agtgcaggag ttatgcgccg
      481 tcgattgagt cagatttctg gaaaccaacc gggtgatcca atcaaaggag tacaacttat
      541 tattcgagcg gttgattcag agaaaatgcc tttgcgttta gccttgggaa atattgcggt
      601 tgatacaatt agtgcgaaac tcaagcaagt gcaagccgat attgaaacgt gggaacaaga
      661 aagtcgggca actgattttt aatttactaa gcaataaaag cagaacatag ctaatttgca
      721 ggcatatttt cccgatatgt taaaccgtat tttttgtttt gcacttttcc tcgttttagg
      781 aaacactatc caagggcaaa cgtttgatcc tccaatactt tcaaacgatc ctactattcc
      841 tgaatgggcg gcaaaaatgt atgctccttc acctaatgtt ttggaagttg atcgcttgtt
      901 tagagcctat tatgcaactc atccattcaa gcaaaacaat tatacacgct attataaacg
      961 ttggagacgt tatgttacgc cctatttaaa tgatgatgga accattcatt atcccgatgc
     1021 aactgagcgt caagctatgg ctgatgcttc gcgttcggtt caggtgcaac gcagcaatac
     1081 gtggagtttc ttaggtccga ttgtacatcg tcgtgggaaa tatgctgtca ctgatgaaaa
     1141 tgtgcagatg tcgtatcatg ccaatgtgta ttgcatcgac cgttcgatga gtaatccaca
     1201 agtgttgtat tgtggaaccg aatgtggcgg agtgtataaa acggttgatc aaggtgtgaa
     1261 ttgggaatat gtgaccgaga ataatatcat tgaagatgtt tctgctatat cggttgatcc
     1321 gctaaacgag aatactgtaa ttttttctgc agcgaatgaa ttttggcgtt cgatagatgg
     1381 tggtgcaaat tggacaattg ttggaaatac agcgttccag aatctgaaca tcaaagcgta
     1441 tcaattcaat tttcatccaa caaatccaca aatcgttttt gcagcaacta atttaggact
     1501 atacaaaagc accgataatg gtataaactg gtcgcagatt tttagtggag aatcgatgtc
     1561 ggttgagttt aaacccggaa attctcaggt cgtttatgcg cttcaattca acgccaatac
     1621 tggaatttcc gatttttata agtcgaccga tggaggaact gtttttacta ttaagcccaa
     1681 cggatggttt agcgttccag cagctgacga aggattaatt gagaataggg gaggacgtat
     1741 tgccgttacg caagctaatc cagaacgtgt gtatgtcttg ttagtgggcg aaagtaatgc
     1801 agctgcaaat ttgcaactta gaggaacgat tggaatttat tcgagtgcga atgcgggtga
     1861 aagttggtcg tttcctcacg gacaacaagg aatgccctat gatgtaaata cacatcctaa
     1921 tttgatggat tttgatggat acagcagtga ttacgatcag atttattaca atacagctct
     1981 cgtatgttcg caattagatg aaacgaaact cttaattgga ggattgaatt tgtggcgtag
     2041 taatgatggt ggagctgcgt atcaaactgt tggtggatac attgggaatt tgcccaatat
     2101 gcacgtcgat ttacaggaat ttaaagttta taaaacgtct gctaacagtg aagaattttg
     2161 gtttagcagc gatggtggaa ttaatttatc gaacgattgg tgtaccacac acgaatctcg
     2221 aactcgcgga atttatgcat cgaatttttg ggggatggat caaggttgga atgatgatat
     2281 catcgttgga ggtcgctacc ataatggaaa tgcagcttat tctcccacat tcgatacagg
     2341 tttattcctt agtataggtg gtggtgaaag tgctactgga tatgtgaatt acagtccgga
     2401 acgaaagacc tatttctctg atatcggcgg acgaaaaatt ccgcttttac aggatgatgt
     2461 gattggaacg tttagcacga atctttatcc caacgaaagt tattacgata atgccagttc
     2521 acgcatgttg ttcgattgga attactggaa tatcgcttac atgggacgcg ataatgtatt
     2581 atacaaaagt accgatggtg gtgcttcttt cggtccattg tatacgtttg gaactgatgc
     2641 tactgctaaa gtttattgga ttgaacaaag tcacgctaat cctttggtaa tgtatgtgca
     2701 acaggtggtt tctaatgtga gcaaactttg gaaaactacc gatggtggaa ttacattctc
     2761 ccaagtcgcc ttaccacaaa gcaaacgtga gctctatttt acattaagtc acaccaatgc
     2821 cgatgaactt tggttagcct tcaccactgg ttccaatgga aataaagttt acacaagcac
     2881 aaatggtgga accttgtgga cgaatatcac ctcatccgca ttaaatggtt tatccataaa
     2941 atcgattgca catcaagctg gaacaaatgg tggcgtttat gtggctactt tccataatgc
     3001 agtgtattat cgttcgaata caaacacaac atggacggtt gtaggtacag gattgccagc
     3061 atccatatat ccgcttcgtt taatgccttt ttaccagaag aataaattac gtttaggttc
     3121 ttggcaattg ggagtttggg aaatagattt ggttgaacca tctgctgtaa tcgccgattt
     3181 ctcttcggat ttttcttcgt tcacttgtcc gggcgatacc ttgtggtttg tcaatcactc
     3241 tgtgtgtgat tcatctgcaa cgtttagttg gtcatttcct ggggcaacgc cttccacatc
     3301 tacgagttta gctccgaaag ttacgtatgc tgcagcagga acatatccag taacattata
     3361 tgtaactcaa aacggacaaa ccttcagcat cactaaggat gcatttatca ccacacaaac
     3421 gtccgcgagc attcctttag cagagaattt cgaaagtggc actttgcctt cggaatggaa
     3481 gttttatgat gatgggaatg atggtgcaaa ctggatcatt agtgatttag caagcagctt
     3541 tggacaagga acgtacaaca tgtttttcga taattactac atcgatgtag ctggtaaacg
     3601 tgatcgattc ttaacgcgta agcttgactt tagttcagca gctgctgttc aattgagttt
     3661 tgatatagct tactcgcaat acgataacaa ttacagtgat tcattagcag tattagtgtc
     3721 gaccgattgt ggaatcactt attcgagtgt atatctcaag ggtggagatg ctttggcaac
     3781 tgctccagat ttaacttctg aacgatttga acccataaat tctcaatgga gaagagatac
     3841 ggttataatt tgggatgcgg caggagcaag tgatgtgatt attgcttttg aaaaccgagg
     3901 tcgttatgga caaccgattt acgtcgataa aatcaacatc caaacgctta gcagtgtgga
     3961 gattccgatt gttccgaatg tgcaggtgga tgtatttcct aaccctgtga gttcatcctt
     4021 aaccttccga ttaaaaggat attcgggcaa gcaagctgat gcagaaatta tcgacgtaaa
     4081 tggtaaacgc tgtaaggagt ttcgagtgct aggttcgcag atggaattat cggtgaacga
     4141 tttagcagca ggaatttatt tccttcgggt taagaccaat tcaggtatga tgcagaagcg
     4201 gtttgtgaag caataatcag ttttcgtgat gaagctttcc tgaacccgaa atggatgtgt
     4261 tgattgttgg ttgtcctttg tagtgaacgt ttccactgcc actgatcgtt acatcgaggt
     4321 gatcgcttac tgtgatttcg gcatcgcccg agccactcac gttaatagtt gattgactcg
     4381 attgcaagcc atacgcgaaa a
//
LOCUS       DAOOQW010000205         4360 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_23879_length_4360_cov_4.605343, whole genome shotgun sequence.
ACCESSION   DAOOQW010000205 DAOOQW010000000
VERSION     DAOOQW010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4360
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23879_length_4360_cov_4.605343"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /locus_tag="PK281_09930"
     CDS             complement(<1..537)
                     /locus_tag="PK281_09930"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOY49701.1"
                     /translation="MLKQNLPNALTLSNLLCGCLAIQQLLAGNPDLVPLLMLLAGVFD
                     FFDGFAARALKVSSPIGKDLDSLADMVTFGVLPSLMAFRLIAETQLTSLLPGEEVAEF
                     FMHFPTSIALLIAVFSAYRLAKFNHDTRQSSSFIGMPTPANALFWAGLWIGCHNGTYT
                     FFNAWTLSGLSVLCSFLLV"
     gene            complement(515..1702)
                     /locus_tag="PK281_09935"
     CDS             complement(515..1702)
                     /locus_tag="PK281_09935"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOY49702.1"
                     /translation="MKRLIKKSHFDNINALRFLAFFGIFVSHCFITGNQQVEVSSLYS
                     DLRQVTGNLNSVCYSLLFLLTGFLNTWTIFEERFIYKKMNLLRFYMRRILGMLPLYFV
                     LFIVGYYILPRVDTGLPEQNFHPIGMGEYLGFYFNFNYQDTGNPLTGVMGNMWSIAVS
                     FQFILIWPILMANFRRKETLLMVLAMVAFIASAWMWSSSDTFRYSSLNIICDFVVGSY
                     LAYFSFFKYKLYIFLKKQTRRTIGFIYLAFFAVIIFIRPIRYELASIPPQLVFIIERI
                     LLTLTLGFFVFEQNFSSNSVFKLAKLKIFNYPGKHAYSMYAYHAIGIIVGYRIMQFMV
                     TEENATSVLLIEPLLAFAVTFALAIFSHEYFENKFTRRKKNYNPTREYNPVGINDAKT
                     KPA"
     gene            1882..2997
                     /locus_tag="PK281_09940"
     CDS             1882..2997
                     /locus_tag="PK281_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HOY49703.1"
                     /translation="MLNIRFLIVLIAIFAQGTTSAQTARKYSNEFLSIGVGARALGMS
                     GAYVSAASDATSGYWNPAGLTGITSKFQGALMHSEYFAGIAKYDYGSLATRLDPKSVM
                     AVSVIRFGVDDIPDTSELIDPNGNINYDRIKSFSAADYGFLFSYARQLKKEGLSVGAN
                     AKVIHRKVGDFAKSWGFGLDAGVQYNVGKWRLAAMARDITSTFNAWSYNLSQSQRDAL
                     SSTGNEIPENSLEVTLPSLTLGGARKFEFGKRFTLLTELDLQTTFDGMRNTIIRDKTF
                     SMSPVFGLEAGYRNIIFLRGGIGNLQTVQNDLNTGRETTFQPNIGLGIRIKGVQIDYA
                     LTDIGDQSTALFSNVFSLRFDINAADKKQNSSTPPSK"
     gene            3006..>3983
                     /locus_tag="PK281_09945"
     CDS             3006..>3983
                     /locus_tag="PK281_09945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49704.1"
                     /translation="MVKRYILPFLFLLLAHSIYAQPFGNEWIQYDQNYYRVKVWNDGI
                     YRIPYTALTEAIPALAGTDPRNIQVFGRGQEQAIFIPGEEDGTWDNTDYVEFFGRKNT
                     GWLDANMYPDANSHSNPNYSLISDTAVYFITWNNSISNLRMIQQTDVNFTGATPAPYF
                     INQVIYQQTSDYYFGKTDFNNSTDPEYTDGEGWSGPVFTAPLLNQITALPSTNAVGTG
                     PQANLTVVYKTISNAPWFNDHRYQMQLSNLPILDTIMEGHGSRKFTFNMNASGLGATS
                     TNFKHSYVGINDNNGVLVTSNSMFSNYVWRYPHNFNLENKTSYRMLLPDN"
     assembly_gap    3984..3993
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4015..>4360
                     /locus_tag="PK281_09950"
     CDS             4015..>4360
                     /locus_tag="PK281_09950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49705.1"
                     /translation="MTNLAASTSTVWLYDLSTGKRITVSLVGGVYKALVPNGSGSEKE
                     LYVAPESQFTTLTANDIRPVNAQGAQPSRFTDLQGFPGVEFLIITNHALGSEASSYKA
                     YRNGKFSTELVYV"
BASE COUNT         1390 a          937 c          814 g         1209 t
ORIGIN      
        1 taccaataaa aaagaacata gtaccgataa gccgcttaat gtccaagcat taaaaaatgt
       61 ataagtgcca ttgtggcaac caatccacaa tcctgcccag aatagtgcat ttgctggtgt
      121 tggcattcct ataaaggaac tactctgacg cgtatcgtga ttgaatttcg ccaaacgata
      181 agcagagaaa acagctatca ataaagcgat agaagtaggg aaatgcatga aaaactcggc
      241 aacttcttct ccgggtaata acgacgttaa ttgagtttct gcgataagtc gaaatgccat
      301 gagtgatggc aaaacaccaa atgtgaccat gtcggccaaa gaatctagat ctttcccaat
      361 tggagaggaa acctttaatg cacgagcagc aaagccatcg aaaaaatcga aaactcctgc
      421 taacaacatc aacaaaggaa ctaagtcggg attccctgcc aacagttgtt gaatagccaa
      481 acaaccgcag agcagattcg acagagttag ggcattaggc aggttttgtt ttagcatcgt
      541 ttatccctac tggattatat tcccgtgtgg ggttgtagtt cttcttgcgt cgggtgaact
      601 tgttctcgaa atactcatga ctaaaaatcg caagtgcaaa agtaaccgca aaggccaata
      661 atggctcaat taaaagcacc gatgtggcat tttcttcggt caccataaat tgcataatcc
      721 gatatccgac aattattcca atggcatgat aagcgtacat gctatatgca tgctttccgg
      781 gataattaaa aatcttgagc ttggccaact tgaataccga gtttgaacta aaattttgct
      841 caaatacaaa gaaccctagc gttaatgtaa gtagtatacg ctcaataatg aaaaccaatt
      901 gaggtggaat gctcgctaat tcatatcgga tcggacgaat gaatatgatt acggcaaaaa
      961 atgccagata aataaaacca atcgtacggc gtgtttgctt ctttaagaag atgtatagtt
     1021 tgtatttgaa aaagctgaaa taagctaagt aagaacctac tacaaaatca caaataatat
     1081 tgagcgagct gtatcgaaat gtatccgatg atgaccacat ccaagccgaa gcaataaacg
     1141 ctaccatcgc caacaccatc aacaaagttt cttttcttct gaaattagcc atcaatatgg
     1201 gccaaatcaa aataaactga aacgatacag caatcgacca catatttccc attactcccg
     1261 ttaatggatt cccggtatcc tgataattaa agttgaaata aaatcccaaa tattcaccca
     1321 tcccaattgg atgaaaattt tgttcaggca agcctgtatc tactcttggt aggatgtaat
     1381 aacctactat aaaaagaaca aaataaagcg gcaacatccc caaaattcga cgcatataaa
     1441 agcgtaaaag gttcattttc ttataaataa acctttcttc gaaaatggtc caagtgttca
     1501 aaaaccctgt tagtagaaag agtaaactgt agcaaacact attaagattg cctgtaactt
     1561 gccgaagatc tgaataaagt gaagaaacct caacctgttg atttcctgta atgaagcagt
     1621 gcgaaacaaa tatgccgaag aacgccagaa accggagtgc attgatattg tcgaaatgcg
     1681 attttttaat aagtcgtttc atgaatcaaa ttcagcatgc atttcgggta ctttatgaaa
     1741 agcaaactga tgcaaaggaa aaaaagtttc tatacctgca aaataattcc aatcggattg
     1801 cgcttaatcg ggtgaaataa cctttcaagt cattccaaac cagacaataa gattatgtta
     1861 aaattcatcc acataaacta catgctaaac attcgcttcc taattgtact aattgcaatt
     1921 tttgcgcaag gaacaacttc tgctcaaaca gctcgcaaat acagcaatga gtttctatca
     1981 attggcgttg gcgcgcgagc ccttggaatg tcaggtgcct atgtttctgc ggcttccgat
     2041 gcgacttcag gttattggaa tccagctgga ttaacaggaa ttacctccaa attccaaggc
     2101 gccctcatgc attccgagta ttttgctggt atcgccaaat acgattacgg ctcccttgct
     2161 acccgtctcg accctaaaag tgtaatggct gtttcggtga ttcgatttgg cgttgacgat
     2221 attcctgata cttccgaact tatcgatcca aacggaaaca tcaattacga ccgcataaaa
     2281 tcattctctg ccgccgatta tggtttccta ttctcctatg cccgtcaact taaaaaagaa
     2341 ggacttagcg tgggtgctaa cgcaaaagta attcaccgta aagtgggcga tttcgctaaa
     2401 tcatggggat ttggacttga tgctggtgtt caatataacg taggaaaatg gcgcttagca
     2461 gctatggcac gagatataac ttcaacattc aatgcttgga gctacaatct gagtcaatca
     2521 caacgtgatg cactttcttc tactggtaac gaaatccctg aaaactcgct tgaagttaca
     2581 cttccctctc ttactcttgg tggtgctcgc aaatttgaat tcggaaaacg tttcacgctc
     2641 ctaactgaac tagaccttca aacaactttc gatggtatgc ggaacaccat cattcgagat
     2701 aaaacttttt ctatgtcacc cgtgttcggt ttagaagctg gatatcgcaa tattatattc
     2761 ctaagaggtg gtattggcaa tctccaaacc gttcaaaatg acttgaatac tggccgcgaa
     2821 actactttcc aaccgaatat cggtttagga attcgtatca agggtgttca aatagattat
     2881 gcactaaccg acattggaga ccaaagtact gcactgtttt ccaatgtttt ttcattacga
     2941 tttgatatca acgctgcaga caaaaaacaa aattcatcga cacctccgtc taagtaggtt
     3001 tgcttatggt taaacgttac atactacctt ttctatttct tcttcttgcc cattctatct
     3061 acgcgcaacc atttggaaac gagtggattc agtatgatca aaactattat cgggttaaag
     3121 tatggaatga tggaatttac cgcatcccat atactgctct tactgaagcc attccagcat
     3181 tggcgggtac cgaccctcgt aatattcaag tattcggtcg aggtcaagaa caagccattt
     3241 ttatacctgg cgaagaagat ggaacttggg ataatactga ttatgtagaa ttcttcggaa
     3301 ggaagaacac ggggtggctc gatgccaaca tgtatcccga tgctaattca catagcaatc
     3361 ccaactattc actcatatca gatactgctg tctatttcat cacgtggaat aactccattt
     3421 ctaatcttcg gatgattcag caaacagatg tgaatttcac aggcgcaact ccagctccgt
     3481 actttattaa tcaagttatc tatcaacaaa ccagcgatta ttatttcggg aaaaccgatt
     3541 tcaacaactc caccgatcct gaatataccg atggcgaagg ttggtctggt ccagtattta
     3601 ccgctcctct actcaaccaa ataacagctt taccgagtac aaatgcagtt ggaactggtc
     3661 ctcaagccaa tctaacggtt gtctacaaaa ccatttcaaa tgctccttgg ttcaacgatc
     3721 acaggtatca gatgcaattg agcaatttgc cgattctcga taccatcatg gaaggtcatg
     3781 gtagccggaa gtttactttc aacatgaacg cttctggctt aggtgctaca tcaacaaact
     3841 tcaagcattc atacgtagga atcaatgata acaatggtgt tttagttact tctaacagta
     3901 tgttttccaa ttatgtatgg agatatcctc ataatttcaa tctagaaaac aaaacttcct
     3961 accgaatgct cttgcccgac aatnnnnnnn nnnggtacga actacttact taatatgacc
     4021 aacctagcgg cgagtacttc taccgtttgg ttgtatgatc tcagcacagg taaaagaata
     4081 actgtttcgc tcgttggtgg tgtttataaa gctctcgttc ccaacggatc aggctcagaa
     4141 aaagaactgt atgttgctcc cgaaagtcaa ttcacaacgc ttacagctaa cgacatccgt
     4201 cctgttaatg cgcaaggtgc tcaaccaagc cgatttacag atcttcaagg ttttcctgga
     4261 gttgaattct taataattac caatcatgct ttaggctccg aagcttctag ttacaaagca
     4321 taccggaacg gaaaatttag caccgaactg gtttatgtag
//
LOCUS       DAOOQW010000206         4309 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_24245_length_4309_cov_4.152562, whole genome shotgun sequence.
ACCESSION   DAOOQW010000206 DAOOQW010000000
VERSION     DAOOQW010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4309
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24245_length_4309_cov_4.152562"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            196..3087
                     /locus_tag="PK281_09955"
     CDS             196..3087
                     /locus_tag="PK281_09955"
                     /inference="COORDINATES: protein motif:HMM:NF015347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BspA family leucine-rich repeat surface protein"
                     /protein_id="HOY49706.1"
                     /translation="MKNLLSLFLVLFAYQATGQAFITTWKTNNFGVSANNQITIPTIG
                     TGYSYSIAWESVDNTASNGTIPGPITGNQTITFPSVGIYRVSITGSFPRIYFDGPNDG
                     FNNSNNDDHDKILTVEQWGSNVWKSMASAFKGCTYLTIPATDAPNLTLVTDMSSMFEG
                     NLTFNQPIEHWDVSSVTDMHKMFSYTSFDQPLKGWDVSSVTDMHEMFFASGFNKPIGT
                     WNVGSVTNMLGMFHSAIYFNKPIETWDVSNVTNMSEMFFQCTRFNQRIGSWDVSSVTD
                     MAGMFSYAPRFNEPIGSWDVSSVTDMSNMFVDATSFNQPIGAWDMSSVTNIAQMFVWA
                     ANFNQPIGAWNVSRVINMSRAFEGATNFNQSLGDWDVSNVISMGQMLNSSGLSIRNYN
                     QTLIGWAAQNVKPNVQLGALGLKYCAGEAARSKLIEDKGWIITGDSKISQPVPLPELS
                     LIHPICFGSTGTITVTIQNTDDAYSFDNGVTFQANNSKSGLTEGKYSVIILSSTGCYS
                     DSTSALINPPLDPPLPIISGSSVVCPNVTAVEYSADNDQYTYTWFVNGGTLVSQQNNT
                     IKVDWGASNFNALVKVLGVDENHCLKDTVTFPIKIQIQLKPDLPMGMDSVCYNFRSGV
                     PYQTSNTNGSMYTWFADGGIVTEGQTTPQVKVDWFDLGQYKLWLKEENTTSTDYCEGY
                     SDTLNVTVFKDLAAITMNFVSVDYEDDEKVQIEWDATLLERISDLIIVSRRIAGSNAP
                     WEIVTTLKKNVQSFLDQNVETDQNSYEYKVEGFNKCDEGLQTVIHNTIKLDGDKDEAQ
                     ELIDLFWNDYNGWNGVERYEVWRKLDADTVYRLIDVTSGDITNYLGKHGADGFVHVLR
                     IKAKKENENTISWSNEVELAFENPIDFIPNIITPNDDDENEYFTIPKLYLYPKNNLSI
                     YDRWGAPVYQRQNYTNDWNADGLPNGTYYYKLHLVKNNKIYKGWIQIVK"
     gene            complement(3143..3430)
                     /locus_tag="PK281_09960"
     CDS             complement(3143..3430)
                     /locus_tag="PK281_09960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49707.1"
                     /translation="MTLDQFTATLEEDAPPQNVTLLLQALWFEANGDWNKAHDLAQEV
                     ESADSSWVHAYLHRKDGDLGNAAYWYQQAGKPVCKKSLHDEWEEIVSAFLS"
     gene            complement(3533..>4309)
                     /locus_tag="PK281_09965"
     CDS             complement(3533..>4309)
                     /locus_tag="PK281_09965"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOY49708.1"
                     /translation="IPIASQSIGEKYHYKQDYTNANNPISKQTAENVSGSFGAGANFN
                     VGAGYMFNEYIGVELNAQYTLGKKIETSNIETYTTGSYSSSTEHIIQHYSRGIYLNPS
                     FILTTARGSKIPYGRFGLVVGSQKIMKDSTYTYTDGFYNSTGTGKGEYSGGISLGFQG
                     AVGINWMLSDKIDLFTEVNFISMTYYAEEYEVTEYNSNGYDYLPELSVSQKKTVFKKE
                     IELGESQNPDKPREELREGSAFSSLSLQVGIRFLLNGHGM"
BASE COUNT         1352 a          828 c          908 g         1221 t
ORIGIN      
        1 gaagaagaac tgaaaaagaa acgcgaagaa gttgatgcat ggaagaaaga gcaaattaaa
       61 tcaagacaac atgaatctga tttttaggaa agcaggtgag agcgaaacac attctttgaa
      121 ttggtttctt taggtctcga catcattcat tgaaatattt tcataatttc gataaccgtt
      181 aatggtaaat ccttaatgaa aaatctcctt tctctctttt tagttctctt tgcgtaccaa
      241 gcaaccgggc aagctttcat caccacctgg aaaacaaaca attttggcgt tagtgcaaat
      301 aaccaaatta ccattccaac aataggcaca gggtatagct acagtattgc ctgggagagt
      361 gtggacaaca ctgccagcaa cggcaccata cctggtccta ttaccggaaa tcaaaccata
      421 accttcccca gtgtaggtat ctaccgcgtt tctattacag gttcatttcc caggatatat
      481 tttgacggac caaatgatgg ttttaacaat agtaataatg atgatcatga taaaatactg
      541 acagtagaac aatggggaag caatgtgtgg aaaagtatgg ctagtgcttt taagggttgc
      601 acctacctga ctataccagc tactgatgcc cccaatctga cacttgtaac agatatgagt
      661 tcaatgtttg aaggtaatct cacatttaat cagcccattg aacattggga tgtgagcagt
      721 gtaacggata tgcataaaat gttcagttac accagtttcg atcaacctct taaaggctgg
      781 gatgtgagca gtgtaacaga tatgcatgaa atgttctttg catccggttt caacaagccg
      841 attggaacct ggaatgtggg gagcgtaacg aatatgctag gaatgttcca ttctgctatt
      901 tatttcaaca agccgattga gacttgggat gtgagcaacg taacaaatat gtctgaaatg
      961 ttttttcaat gcacaaggtt caaccagcga atcggatcct gggatgtgag cagcgtaacg
     1021 gatatggctg gaatgttttc ttatgctccc aggttcaacg aacctattgg aagttgggac
     1081 gtgagcagtg taacagatat gtcaaatatg tttgttgatg ccactagttt caaccaaccc
     1141 attggagctt gggatatgag cagcgtaacg aatatagcac agatgttcgt atgggcagct
     1201 aacttcaatc agccaatagg agcctggaat gtaagcaggg taataaatat gagtcgcgca
     1261 tttgaggggg ccacaaattt caaccagtcg cttggagatt gggatgtgag caacgtaata
     1321 tcgatggggc agatgttaaa ctcaagtggt cttagtattc gcaattacaa ccagacctta
     1381 attggctggg cagcgcaaaa tgtaaaaccc aatgttcaat taggggcgct tgggttaaaa
     1441 tattgtgccg gtgaggcggc tcgttccaag ctgatagagg ataaaggttg gatcataaca
     1501 ggggatagta aaattagcca acctgtacct ttacctgagc tttcactcat tcatcccatt
     1561 tgctttggtt ctactggaac cattactgtt accattcaaa atacagatga tgcttacagc
     1621 tttgataatg gcgtaacttt ccaagcgaac aatagcaaat cgggactgac tgagggtaag
     1681 tattccgtca taattctaag tagcaccggc tgctattcag acagtacatc agctttaatc
     1741 aatccacctc ttgatccgcc attacctatt atctctggca gctccgtagt atgccccaat
     1801 gttactgctg ttgagtacag tgcagataat gatcaatata cttatacctg gttcgttaat
     1861 ggcgggactc tagtgtcaca acaaaacaat acgataaaag tagattgggg cgcaagtaat
     1921 ttcaatgctt tggtgaaagt tcttggtgta gatgaaaatc attgtttaaa agataccgtc
     1981 accttcccga ttaaaatcca aatacagctc aagcctgatt tacctatggg aatggattcg
     2041 gtgtgctata acttcagatc aggcgtacct tatcagactt caaataccaa tggatcaatg
     2101 tatacatggt ttgctgatgg cggtattgta acagaaggac aaacaacacc acaagtcaaa
     2161 gtggattggt tcgatttggg tcaatataaa ctgtggttaa aagaagaaaa caccaccagc
     2221 acagattatt gtgaaggtta ttcggatacg ttaaacgtta ctgtgtttaa agatttggct
     2281 gcgatcacaa tgaattttgt gagcgtagat tatgaggatg acgaaaaagt gcagatcgaa
     2341 tgggacgcta cattgcttga gcggatctcg gatttgataa tcgtcagtag gagaattgcc
     2401 ggaagcaacg caccctggga gattgttacc acgctgaaaa aaaatgttca atctttttta
     2461 gatcagaacg ttgagaccga ccaaaatagt tatgaataca aagtagaagg atttaacaaa
     2521 tgcgatgaag gtcttcagac ggtaatccac aacaccatta agttggatgg cgacaaagac
     2581 gaagcgcaag aattgataga cctcttctgg aatgattaca atggatggaa tggcgtagaa
     2641 agatatgaag tctggagaaa attagatgcc gataccgtgt acagacttat tgatgtaacc
     2701 tcgggcgaca tcaccaatta tttagggaaa catggtgccg atggatttgt gcatgtgctg
     2761 aggatcaagg ccaagaagga aaatgaaaac acgatatcat ggtctaacga agttgaactt
     2821 gctttcgaga atccaataga ttttattccg aacatcatca ccccgaatga cgatgatgaa
     2881 aacgaatact tcacaatacc taaattatac ctatacccta aaaataattt gagcatttat
     2941 gaccgttggg gtgcaccggt ttatcagaga caaaattata ctaacgattg gaacgctgat
     3001 ggcttaccca atggcaccta ctactataag ctacatctcg taaaaaacaa taaaatctac
     3061 aagggttgga tccagatagt gaagtagagt aatactcctt cgcgaaagaa caagtcaaat
     3121 caaaacaaca cgaatccgat ttttaggaaa gaaatgcaga aacgatttct tcccactcat
     3181 catgcagcga ttttttgcaa actggtttac cggcctgctg ataccaataa gctgcattac
     3241 ctaaatcacc atctttgcga tgaaggtagg catgaaccca cgatgaatct gccgactcaa
     3301 cctcttgtgc tagatcgtgc gctttgttcc aatcaccatt ggcctcgaac caaagagctt
     3361 gtaataacag ggtaacgttt tgtggaggag catcctcttc caatgttgct gtgaattgat
     3421 caagtgtcat ggtgcaaagg taatttttgt gtcatgtttt agtagtgtct tgacaaaaaa
     3481 aattccagag aaccgtacta ctctggaatt attttcaata cgcaaacttt aattacatcc
     3541 cgtggccatt caataaaaac cgaataccga cctgaagcga taatgaacta aaggcacttc
     3601 cctcgcgtaa ttcttctctt ggcttgtctg gattttgtga ttcacctaat tcaatctctt
     3661 ttttaaatac ggttttcttt tgtgaaactg agagctcagg caaataatca tagccattgg
     3721 agttatattc tgttacctcg tattcttctg cgtaataggt catgctaatg aaattcactt
     3781 cagtgaataa atcaatcttg tcgctcagca tccaatttat accaacagcc ccttgaaaac
     3841 caagactgat acctccagag tattctccct tgccagttcc tgtagagttg tagaaaccat
     3901 cagtatacgt gtaagttgaa tccttcatta ttttttggga tccgacaact aaaccaaacc
     3961 gaccgtatgg aattttcgat cctctggcgg tagttagtat gaaagaagga tttaaataaa
     4021 tacctctgga atagtgctgg atgatatgct cggtagaact ggaatagctg ccagtggtat
     4081 aggtttcgat attactggtt tcgatttttt ttcctaatgt atattgagca ttaagttcga
     4141 caccaatata ctcgttgaac atataacctg cacccacatt aaaatttgcg ccagcgccaa
     4201 aagatcctga aacattttca gctgtttgtt tgctaatggg attgttagca ttcgtataat
     4261 cttgtttgta atgatatttt tcaccaatag attgtgatgc aataggaat
//
LOCUS       DAOOQW010000207         4283 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_24445_length_4283_cov_4.429044, whole genome shotgun sequence.
ACCESSION   DAOOQW010000207 DAOOQW010000000
VERSION     DAOOQW010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4283
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24445_length_4283_cov_4.429044"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /gene="pgi"
                     /locus_tag="PK281_09970"
     CDS             <1..275
                     /gene="pgi"
                     /locus_tag="PK281_09970"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016169968.1"
                     /note="functions in sugar metabolism in glycolysis and the
                     Embden-Meyerhof pathways (EMP) and in gluconeogenesis;
                     catalyzes reversible isomerization of glucose-6-phosphate
                     to fructose-6-phosphate; member of PGI family; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HOY49709.1"
                     /translation="RVFEGNRPTNSFLIKRINAFNLGALIAAYEHKIFVQGVIWNIFS
                     YDQWGVELGKQLANRILPELKTNELINSHDSSTNLLINRWKAMKSE"
     gene            314..757
                     /locus_tag="PK281_09975"
     CDS             314..757
                     /locus_tag="PK281_09975"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOY49710.1"
                     /translation="MTTATLTTQYGDMKIEFFDKDAPITVDNFVRLAKSGFYDGLTFH
                     RVIPDFVVQGGDPTGTGAGGPGYSIKCELTGDNQYHDRGVLSMAHAGRDTGGSQFFIC
                     HSRRNTSHLDRKHTCFGKVTEGVDIVDQIRQGDKIEKIVVHESAE"
     gene            760..1689
                     /locus_tag="PK281_09980"
     CDS             760..1689
                     /locus_tag="PK281_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018942876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HOY49711.1"
                     /translation="MYSLAIHGGAGTLARHQFTPEKEAAYHAALASALLAGETVLKNN
                     GSSLDAVEAAVRSLEDCPLFNAGRGAVFTHDKTHELDASIMEGKNLMAGAVAGVSGIA
                     HPVSLAKTIMQQSEHVMLCGKGAEQFAVEHGFALESPEYFYTELRYNQLMQVIDESGA
                     HLDHSVELPPDEKKFGTVGAVALDVHGNLAAATSTGGMTNKRWGRAGDSPIIGAGTYA
                     NNTTCAISCTGHGEFFIRSVVAYDISCLMEYAGLSLEDACRKVVNDKLVKFGGEGGLV
                     AIDKHGNICLPFNSEGMYRGWKSANSDTFTAIF"
     gene            1699..4128
                     /locus_tag="PK281_09985"
     CDS             1699..4128
                     /locus_tag="PK281_09985"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HOY49712.1"
                     /translation="MTQLFRKLLIGVPLLTAVSLFAQKEPVKPFIQSLPYDSASVPRE
                     RFVDFQHLRLEASFEPTQGKVNGWVTHRFQAIRQNVDTLFFDGPDIQIQEVNYHDQKL
                     TIRKYETGFTVVFPKALSWNSADSLSIRYVAFPRHGLYFVGWNDPTNRARKQIWSQGQ
                     GTDNRCWLPMFDNMNDKITSEMIITMAKPFKVLSNGVKLGVKDVGANQIWHYRMNKPH
                     APYLIMLGIGDYEIDARKTGSGLAVNLWYYPDWKDRVAGTYAKSTEMIEFFEKEIGVP
                     FPWENYSQIPVQEFMYGAMENTTATVFGDFFFNDARGLLDRSYVGVNAHELAHQWFGD
                     MVTARSETHHWLQESFATHYNMMYEREAFGQDYFDLARRNAQNAALNASTRDLYPVAS
                     SDAGSTRWYPKGAVVLEMLKYVVGREEFNRAINHYLKSHAYDNVDTEDLLIAFHETLG
                     YSLNWFWDQWIYRGGEPVYKVESESTKESTRFIVSQLQEKLPYVGLFKMPIVFEVHFT
                     DGSKESKTVWISEKVHNVKIDHPAGKIAAYALFDPGNQVIKAVVFNKSFDQLKQQALS
                     AQYMLDRLDAVIAMSDLPIDQKRDVLIQVYKKETFHEVREAVIEQLAKDKNKKSRQLM
                     ASSFTDSDLIIRKAAVASYDTIPKDFKKSYEGLLNELSYQLQEEVLYKLCLSFPGDRA
                     TYLEKTKSEMGNNAHNVRITWLEWSIKNGNKAAITELADLASVSFEFNTRRGALTLAK
                     ELSIFNEDFLKHALDAARSANNRMSSAGVGYLNWAYKLNPEWKTMIDSQLNTWNAQQK
                     WEYDLAKKIQK"
BASE COUNT         1285 a          835 c          932 g         1231 t
ORIGIN      
        1 tccgagtttt cgaaggaaat cgaccaacca atagttttct aatcaaacgc atcaacgcct
       61 ttaatctagg ggctttgatt gcagcatacg aacacaagat tttcgttcaa ggtgttatat
      121 ggaacatctt cagttacgat caatggggcg ttgaactcgg taaacaacta gccaatcgca
      181 tccttcccga attgaaaaca aatgaactca tcaacagcca cgattcttct actaatttgc
      241 tcatcaaccg atggaaagca atgaagtctg aatgattgtc ggcgaatact atctttgcgc
      301 aaaattttaa ttcatgacta ctgcaactct tactacacag tacggcgata tgaagattga
      361 attcttcgat aaagacgcgc ctatcacggt agataatttc gttcgcttag caaaaagcgg
      421 attttacgat ggacttacat ttcaccgtgt aattcctgat ttcgttgttc aaggtggcga
      481 tccaactgga acaggcgcag gtggaccagg ttacagcatc aaatgcgaac taacgggcga
      541 taatcaatat cacgatcgtg gagttctttc tatggctcat gctggtcgcg atacaggtgg
      601 atcacagttt ttcatttgcc acagccgtcg caatacttct cacttagacc gtaaacacac
      661 gtgttttggt aaagtaaccg aaggtgttga tattgttgat caaattcgtc aaggcgataa
      721 gattgaaaaa attgttgttc acgaaagcgc tgagtaatcg tgtatagcct cgccattcat
      781 ggtggagccg gaacgctcgc ccgccatcag ttcactcccg aaaaagaagc tgcttatcat
      841 gcagctcttg caagtgccct gttagcgggc gagactgttt tgaaaaataa tggatcatca
      901 ctcgatgcgg ttgaagctgc tgtgagatct ttagaagatt gtccgctatt taatgccgga
      961 cgaggagccg ttttcaccca tgacaaaacg catgaattag atgcgtcaat tatggaagga
     1021 aaaaatctta tggctggagc agttgctggt gtttctggca ttgctcatcc tgtttcactt
     1081 gccaaaacca tcatgcagca atccgaacat gtgatgctgt gtggaaaagg agctgaacag
     1141 tttgctgtcg aacacggatt tgcactcgaa tcacccgaat atttttatac cgaactgcgc
     1201 tacaatcagc tcatgcaggt gattgatgaa agtggagctc atctcgatca ttcggtagaa
     1261 ttacctccag atgaaaagaa atttggaacg gttggcgcag ttgcgttgga tgttcatgga
     1321 aatttagctg cagctacttc aactggcgga atgaccaata aacgctgggg cagggcagga
     1381 gacagtccaa ttattggtgc cggaacatac gcaaacaata ctacctgcgc catttcctgt
     1441 accggacacg gtgaattttt cattcgttca gttgttgctt atgacattag ttgcttgatg
     1501 gaatatgcag gattgagttt ggaggacgcc tgtagaaagg tagtcaacga taaactggta
     1561 aaatttggtg gcgaaggcgg attagttgcc atcgataaac acggaaatat ctgtttacct
     1621 tttaattccg aaggcatgta tcgtggctgg aaatccgcta attctgatac ttttacggcg
     1681 atcttttaag accgctatat gacccaactc tttcggaaac ttctaattgg agttccttta
     1741 ttgacggctg tttcgctttt tgcgcaaaag gagccggtaa aaccatttat ccaatcgctg
     1801 ccttacgatt ctgcatccgt tccgcgtgaa cgcttcgttg atttccaaca tttgcgcttg
     1861 gaagcttcct ttgaaccaac gcaaggaaaa gtaaacggtt gggtaactca tcgttttcag
     1921 gcaatccgcc aaaatgtgga tacactcttt ttcgatggtc ccgatatcca gattcaggaa
     1981 gtgaattacc acgatcaaaa acttacaatc cgtaagtatg aaacgggttt caccgtagtt
     2041 tttccgaaag cactcagctg gaattcggcc gatagcttaa gcatacgtta tgttgctttt
     2101 cctcgtcacg ggctttattt cgtaggctgg aacgatccaa caaatcgtgc tcgtaaacaa
     2161 atctggtcgc aaggacaagg aaccgacaat cgttgctggt tgccgatgtt cgataacatg
     2221 aacgataaga ttacttccga aatgatcatt acgatggcaa aaccctttaa ggtattgtcg
     2281 aatggtgtaa aactgggagt taaagatgtt ggagcaaatc aaatctggca ctaccgcatg
     2341 aataagccgc atgctccgta tttgattatg ttaggaattg gtgattatga gattgatgca
     2401 cgtaaaactg gttctggttt agcggttaac ctttggtatt atcccgattg gaaagatcgc
     2461 gtagctggca cctatgcaaa gagtaccgaa atgatagaat tctttgaaaa agagattggt
     2521 gttccgtttc cttgggaaaa ctacagtcaa attccagttc aagaatttat gtatggagcg
     2581 atggaaaata cgactgcaac agtatttggt gatttcttct tcaacgatgc ccgtggatta
     2641 ctcgaccgtt cctatgtagg tgtgaatgca catgagcttg cacatcaatg gtttggtgat
     2701 atggtaacag ctcgatcgga aacacatcat tggcttcagg aaagtttcgc cacacactat
     2761 aacatgatgt atgagcgaga agcctttgga caagattact ttgatttagc aagacgaaac
     2821 gcccaaaatg cagctttaaa tgcatctact cgtgatttat atccggttgc aagtagcgat
     2881 gctggaagca cacgctggta tcccaaaggt gcagtagtgc ttgaaatgtt gaagtatgtg
     2941 gttggacgtg aagaatttaa tcgtgctatc aatcattact tgaaatcgca tgcttacgat
     3001 aatgtcgata cggaagattt actcattgca tttcacgaaa ctttgggtta ttcgctcaat
     3061 tggttttggg atcaatggat ttatcgtggt ggagaaccag tttacaaggt agaatccgaa
     3121 agcaccaaag aatctacacg tttcatcgtt tctcaattac aagaaaaact accatatgta
     3181 ggtttgttca aaatgcccat cgtgtttgaa gtgcatttta ctgatggaag taaggaaagc
     3241 aaaacggttt ggatttcaga aaaggtgcac aatgttaaaa ttgatcatcc tgctggaaag
     3301 attgcggcat atgctctttt tgacccagga aatcaggtta ttaaggcagt tgtattcaac
     3361 aaatctttcg atcaattaaa acagcaagcg ctttcagctc aatacatgct cgatcgctta
     3421 gatgcagtaa ttgctatgag cgatttacca atcgatcaaa agcgcgatgt gttgattcag
     3481 gtttataaaa aagaaacatt tcatgaagta cgcgaagcgg ttatagaaca gcttgctaag
     3541 gataaaaaca aaaaatctcg tcaattaatg gcttcatcat ttactgattc agatttgatt
     3601 attcgaaaag ctgctgttgc ttcatatgat acaatcccga aagactttaa gaagtcttac
     3661 gaaggattac tcaatgaact tagctaccaa cttcaagaag aagtattgta caaactatgt
     3721 ttgagttttc ctggcgatcg tgcgacttac ttggaaaaaa cgaaatcaga aatgggcaac
     3781 aatgctcata atgtgcgcat tacttggctt gaatggtcaa tcaaaaacgg aaacaaagct
     3841 gcaatcaccg aattagccga cttagcttcc gtttcttttg agtttaacac cagaagaggt
     3901 gctttaacat tggcaaaaga gttaagtatt ttcaatgaag atttcctaaa acatgcgctg
     3961 gatgctgctc gtagcgcaaa caaccgaatg agcagtgctg gagttggtta tctcaattgg
     4021 gcgtataaat taaatcccga atggaaaacc atgatcgatt ctcagttaaa tacttggaac
     4081 gcacaacaga aatgggaata tgatttagcg aagaaaattc aaaagtaaaa ttgaaatctt
     4141 ttactctaaa acgcgctgca aactcaattg tagcgcgttt ttcagtttat ataggtgatt
     4201 attcataagc atctgaagtt tatgaaataa cttattgaat ttcatttctt ttcattccat
     4261 tttaattcac ctaataaact aaa
//
LOCUS       DAOOQW010000208         4273 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_24520_length_4273_cov_5.671882, whole genome shotgun sequence.
ACCESSION   DAOOQW010000208 DAOOQW010000000
VERSION     DAOOQW010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4273
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24520_length_4273_cov_5.671882"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..702)
                     /locus_tag="PK281_09990"
     CDS             complement(<1..702)
                     /locus_tag="PK281_09990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49713.1"
                     /translation="MKRLFFLICLCIGSEISLQAQVDSSGKKPITWTKELREVTIRDV
                     RKPDTLIQSPTWMIRDFHFRGDSLVLLTWEKNPDKCTIRLFDRNGKELASHKLADKPL
                     GFNKDVYDKLYLECKSATYRLDFYSGAIYLQAMEEKFYYSAIRPTVAANDELFFVSSF
                     HPQRPDFVFLKTERMSRETDTLLEVRDQHLYDLYYSEYKFLPFKTQCAIKRLSRETGQ
                     SKYDIAAQFSGFTQSL"
     gene            971..1504
                     /locus_tag="PK281_09995"
     CDS             971..1504
                     /locus_tag="PK281_09995"
                     /inference="COORDINATES: protein motif:HMM:NF024911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOY49714.1"
                     /translation="MTKRILLTGGPGFGKSSIIQELERMEYAVFHEVARQIIQDEIAS
                     GGSAVPWLDIAAFSAQVLQGRIDQYAAGENGLWFYDRGLPDIAGFLRKEKKETPEDVW
                     QLCQDFKYETQVFITPPWEAIFHRDAERKEDFAAAIKVHKALEAVYTELGYELIPVPM
                     GSIRWRAQYILNKAGIK"
     gene            1516..3426
                     /locus_tag="PK281_10000"
     CDS             1516..3426
                     /locus_tag="PK281_10000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HOY49715.1"
                     /translation="MGKPREILERFWGFNTFRPLQEEIVQSVIDGHDTLALLPTGGGK
                     SICFQVPALCLEKLCIVISPLIALMKDQVENLEKKGIQAGMISSSMKALEIDMLIDRC
                     QSGQMQFLYVSPERLKSERFREVLPNLNIGMIAVDEAHCISQWGYDFRPSYTEIAQIR
                     ELLPDIPIIALTATATPEVCDDIEKQLQFKNSKRFKKSFTRSNLAYIVRFTEDKTTQL
                     LRILQHVSGSSVVYVRNRKRCKEISEMLLQAGISASYYHAGLDGATRNYRQLQWIEGR
                     VRVMVCTNAFGMGIDKPDVRSVIHLEMPDSPEAYFQEAGRAGRDGEKAFAVLLSDEGD
                     ERDFEDRFNYAFPGFDTIKAMYNWLGNYLQLPVGSGQDQTFAFDLGLFAKRYKHHPVE
                     CYSALRFLEREGLISLQENFYQPSRIFIPVNQQEIYYFEFQNPTYEAVLKTLLRSYGG
                     LFQEYVQIREQELAERIQVPIARLIEMLNYLDKSGMISYYPAINESQIYFTLARQHPD
                     RIPISLKRYEERRTAALTRMQAMMRYVKETQTCRSKYLVDYFGEIDALRCGQCDVCLE
                     QAKAGKYEAWKDRMLLKIQQLLVVEEQSPEQIIEQLPDNPAVILEAIREWIDEGILKE
                     TETGLIRWNTPN"
     gene            3408..4004
                     /locus_tag="PK281_10005"
     CDS             3408..4004
                     /locus_tag="PK281_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HOY49716.1"
                     /translation="MEYTQLTRDWYLGEDVVKQAEFLLGKVICTQINGNYCSGIISET
                     EAYAGEIDQASHAFGGRRTARTETMYQTGGIAYIYLCYGMHHLFNVVTAPEGIPHAIL
                     IRGIQPLEGIPIMQQRRNGKAIGKNFSSGPATASQALGLHTSLNGISCLESTIWIEDR
                     NIPIRSEYISKGPRIGVDYAGDHAKWPYRFWLNQAIEI"
     gene            4032..>4273
                     /locus_tag="PK281_10010"
     CDS             4032..>4273
                     /locus_tag="PK281_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49717.1"
                     /translation="MKFINRYFGIIWGIASFLAVLFWMILLKRFDWEQWIEYSSCVCA
                     LYVFYSSRSFRGNKFRYITMASTLSLIGLGLRVMRWA"
BASE COUNT         1293 a          803 c          923 g         1254 t
ORIGIN      
        1 cagcgattga gtgaatcctg aaaattgagc tgctatatcg tatttacttt gtccagtttc
       61 tcgacttaag cgcttgattg cacactgagt tttaaagggc aagaatttat attctgaata
      121 atataaatca tataaatgtt gatcacgaac ttcaagcaat gtatcggttt cacgactcat
      181 tcgttcggtt ttcaaaaaca caaaatcggg acgttgagga tgaaaactac tgacaaagaa
      241 taactcgtcg tttgcagcaa cagtcggacg aatagcactg taataaaact tctcttccat
      301 ggcttgcaga taaatagctc cggaataaaa atccaagcga taagtggcag atttgcactc
      361 gagatacagt ttatcgtaca cgtctttatt gaatcccagc ggcttatccg caagtttgtg
      421 tgaagccaat tcctttccat ttcgatcaaa taaacgtatg gtgcatttat caggattttt
      481 ctcccaagta agaagtacta aactatctcc ccgaaaatga aaatctcgaa tcatccaagt
      541 aggcgattga attaatgtat caggcttacg aacatctcga atggtcactt cgcgtagttc
      601 tttggtccaa gtaattggct ttttaccgga agaatccact tgtgcttgaa gtgaaatttc
      661 actcccaata cacagacaaa taagaaagaa aagtcttttc ataacgattg tttttggggt
      721 gattttgact agtaaaaagg tatggtgcta attggtagat tgaaaaaaca catttagtac
      781 agtatcgctc aatcacatgc atttttcatg taataatcaa attgaaatac actcttgtag
      841 cttgataatg agttgctaag actagaacat ttatatcctt tccgacaaaa aaatacctcc
      901 aaaaatcttt cttctaaaaa gcctgcaaac caataggaaa aggcggtcaa ctgcatttac
      961 tttgcaaacg gtgaccaaac gcattctcct cacaggcgga cccggattcg ggaaatcaag
     1021 tattatccaa gaattggagc gaatggaata tgcggttttc cacgaagtag cgcgacaaat
     1081 catacaagac gaaattgctt caggaggctc ggctgttcct tggttagaca ttgcagcttt
     1141 ttcggctcag gttttacaag gtcgtatcga tcaatatgct gcaggtgaaa atggcttgtg
     1201 gttttacgac cgtggattgc ctgatattgc tggattttta cgcaaagaaa aaaaagaaac
     1261 acccgaagat gtttggcaat tgtgtcagga ttttaaatac gaaacacagg tcttcatcac
     1321 accaccttgg gaagctattt ttcatcgcga tgcggagcgg aaagaggatt ttgcagctgc
     1381 cattaaagtt cataaagcct tagaagccgt ttatacagaa ttaggatatg aattaattcc
     1441 cgttcccatg ggttctattc gttggcgtgc acaatacata ttgaataaag caggaatcaa
     1501 gtaaatttgg tcctgatggg aaagccacgc gagatacttg agcgattttg gggatttaac
     1561 acctttcgtc cgcttcagga agaaatagta caatctgtta tcgacgggca cgatacactt
     1621 gcgctattgc caactggtgg tggaaagtcg atctgctttc aagttccagc actgtgcctc
     1681 gaaaagcttt gtattgttat ctctcctttg attgctctga tgaaagatca agtggaaaat
     1741 ctggaaaaga aaggcattca agctggaatg atttcatctt ctatgaaagc gcttgagatt
     1801 gatatgctca tcgatcgctg tcagtcggga caaatgcaat ttctctatgt ttcgcccgaa
     1861 cgcttaaaga gtgagcgttt tcgtgaagtc ttgcccaatt tgaatattgg gatgatcgct
     1921 gtggatgaag cgcattgtat ctcccaatgg ggatatgatt ttcgtccgag ctataccgaa
     1981 attgctcaaa tacgcgaact tttaccggat attccaatca tagcactcac tgctactgct
     2041 actcccgaag tatgtgatga tattgagaag cagctacaat tcaagaattc caaacgattt
     2101 aagaagagtt ttacccgttc taatctggcg tatatcgtac gatttaccga agataaaaca
     2161 actcaattac tgcgaatttt acagcatgtt tcaggtagtt cggtcgtgta tgttcgtaac
     2221 cgaaaacgct gcaaggaaat ctccgaaatg ctactacagg cgggaatttc agcttcttat
     2281 tatcatgcag gtttggatgg tgcaacgcgt aattatcgcc agctacaatg gatcgaaggt
     2341 cgtgttcggg taatggtgtg tacgaatgct tttggaatgg gcatcgataa acccgatgtg
     2401 cgtagcgtga tacatcttga aatgcccgat tcacctgaag catattttca agaagcagga
     2461 cgagcggggc gtgatggtga aaaagccttt gccgttttgc ttagcgacga aggtgatgag
     2521 cgtgattttg aagatcgatt taattatgct ttccccggtt tcgacaccat caaagccatg
     2581 tacaattggt taggtaatta cctgcaattg ccggttggtt cgggacaaga ccaaacattc
     2641 gcattcgact tgggattatt cgccaaacga tacaaacatc atccggtaga atgttattct
     2701 gcacttcgat ttctcgaacg agaaggcttg attagtttgc aggaaaattt ctatcagcca
     2761 tcgcgcattt ttattcctgt caaccaacaa gaaatctatt atttcgaatt ccaaaatcca
     2821 acctatgaag ctgtattaaa aacactgctt cgctcctacg gtggcttgtt tcaggaatat
     2881 gtgcaaatac gcgaacaaga attagctgaa cgcattcaag tacctattgc gcgcttaatt
     2941 gaaatgctga attacttaga taaatcgggc atgatcagtt attatccggc tattaacgaa
     3001 tcacaaattt atttcacgct tgcccgccaa catcccgatc gaattcccat ttcactcaaa
     3061 cgttacgaag aaagaagaac agctgcactc acacgtatgc aagctatgat gcgctatgtg
     3121 aaggaaacac aaacatgtcg ctcgaaatat ttagtggatt attttggaga aatagatgca
     3181 ttgcgttgcg gacaatgtga tgtgtgttta gagcaggcga aagcaggaaa gtatgaagct
     3241 tggaaagatc ggatgttgtt gaaaattcag cagttattgg tggtggaaga acaatcaccc
     3301 gaacaaataa ttgagcagct tcccgataat ccagcagtga tattagaagc tattcgtgaa
     3361 tggattgatg aaggaatttt gaaagaaact gaaacaggat taattcgatg gaatacaccc
     3421 aattaacgcg tgattggtat ttaggcgagg atgtagttaa gcaagcggaa tttctgttag
     3481 gcaaagtgat ttgcacccaa ataaatggaa actattgtag cggaattatc agtgaaaccg
     3541 aagcttatgc gggagaaatt gatcaagcga gtcatgcttt tgggggaaga agaactgctc
     3601 gcacagaaac catgtatcaa acgggtggaa tagcctacat atatttatgt tacggaatgc
     3661 atcatttatt taatgtagtt actgctccgg aaggaattcc tcatgcgata ttaattcgtg
     3721 gaattcaacc tttagaaggt attccaatta tgcagcaacg tagaaatgga aaagcaattg
     3781 gaaagaattt tagttctggt ccagcaacag cttcgcaagc tttaggttta cacacttcct
     3841 taaatgggat ttcttgtctc gaatcgacca tttggattga agaccgaaac attcccattc
     3901 gttcagagta tatttctaaa ggaccacgca ttggtgttga ttatgctgga gatcatgcaa
     3961 aatggcccta tcgtttttgg ttaaaccaag ctattgagat ttaacttata cttgaattct
     4021 gttcgaaaac catgaaattc atcaatcgct attttggaat aatctgggga attgcctctt
     4081 ttctagcggt tttattttgg atgatattat tgaaacgttt cgattgggaa caatggatcg
     4141 aatacagttc atgtgtttgt gcgctttatg tgttttatag cagtcgttct tttcggggaa
     4201 ataagtttcg ctacatcaca atggcatcca cattatcact gataggctta ggtttgcggg
     4261 tgatgagatg ggc
//
LOCUS       DAOOQW010000209         4266 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_24573_length_4266_cov_5.304441, whole genome shotgun sequence.
ACCESSION   DAOOQW010000209 DAOOQW010000000
VERSION     DAOOQW010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4266
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24573_length_4266_cov_5.304441"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /locus_tag="PK281_10015"
     CDS             <1..627
                     /locus_tag="PK281_10015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49718.1"
                     /translation="YNFVHTMNNRGVPDTLRIDGLSTYRIVAHTLPPVEKKDVQLVAG
                     KHTIIPIDAPQGFLILKAGEGMTYRDLQCIIRKAGDMNTLDVMSFKDTRKLIVGKYDL
                     EILTLPRTNLKAVDIAQSHTTTVKIDEPGTVNFQLPLQGIASVLLEDKGQMIWVCNLS
                     ENTQQEVVRLQPGKYKAVFRGKGVRETLFTIEKNFIVQPGTSQQVVLK"
     gene            633..1586
                     /locus_tag="PK281_10020"
     CDS             633..1586
                     /locus_tag="PK281_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008612574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOY49719.1"
                     /translation="MEPFTFTDQNDTRSGFGAGLHELGKSNPNVVALCADLIGSLKMD
                     AFVKDFPERFFQAGIAEANMIGMAAGLTIGGKIPFTGTFANFSTGRVYDQIRQSVAYS
                     GKNVKICASHAGLTLGEDGATHQILEDIGLMKMLPHMVVINPCDYNQTKAATIAIADY
                     DGPVYLRFGRPKVPNFTPADQKFEIGKAVILKEGKDVSIFATGHLVWKALKAAEALWE
                     KGIDAEVINIHTIKPLDDEAVIASVGKTGCAVSAEEHQRNGGLGDSIAQLLARTLPRP
                     LEMVAVNDSFGESGTPEELMVKYGLDTVNIIEAAESAIRRR"
     gene            1618..2166
                     /locus_tag="PK281_10025"
     CDS             1618..2166
                     /locus_tag="PK281_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013622119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOY49720.1"
                     /translation="MEEFSDRELLDLFAQASTRNHAFNMLVRQYQQRLYWHIRKMVLD
                     HDDADDLLQNVFIKAFKALDNFREDSKIYTWLYKIATNECITFLNTKKKRFMVPMADV
                     EYQLSQNLETDSYFSGDRIQMKLQQAILTLPDKQRLVFNLKYFEEMKYEDMSEILGTS
                     VGALKASFHHAVKKIEEFMKRD"
     gene            2199..2648
                     /locus_tag="PK281_10030"
     CDS             2199..2648
                     /locus_tag="PK281_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49721.1"
                     /translation="MNPEETPDFIPEGFPKGNPFRVPEGYFDRFPAKLAERMEEDKKI
                     RRFPFPSLLKPAPLLAAACMLIIAGVFGYRMLSGPADPLTDDEISTYVYQEGIIDELE
                     VDDILESTDMAESTDTTPTRAYTNENEEIQYYLLDEDIDDADIINEL"
     gene            2659..3117
                     /locus_tag="PK281_10035"
     CDS             2659..3117
                     /locus_tag="PK281_10035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49722.1"
                     /translation="MKLQLIILLLILNFPGIAQKDNVRERIKSQRIAFITQKLALTPD
                     EAQKFWPVYNQYSDEIDRVKKDMNKLRRSTNDNLMSMNDKDIEKALEDDLQNQQKITD
                     LQRKYQSELKKVIPARKIAQLYKAERDFKLTLLKRMRDSGHQGPPPEEEL"
     gene            complement(3195..>4266)
                     /locus_tag="PK281_10040"
     CDS             complement(3195..>4266)
                     /locus_tag="PK281_10040"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOY49723.1"
                     /translation="AHQHTSTPAHQHTSTPAHQHTSTPAMLTIDIHTHILPEHIPNFK
                     ERFGYGGFIHLDHHKPCCARMMKDDQFFREVQDNCWDPKARMHECEHHGVHMQVLSTV
                     PVMFSYWAKGEDALAVSEFLNDHIAGIVRDYPDRFIGLGTIPMQSPKLAIRELERCMK
                     ELGMAGIQIGSNVNQLNLNEPEFFEIFQAAEELGAAIFVHPWEMFGQKDIQRYWLPWL
                     VGMPAETSRAICSMIFGGIFERLPKLRVAFAHGGGSFPLTLGRIKHGFDCRPDLVAID
                     NAVSPEKYLNRLYFDGLVHDEAAMRYLIDFAGVERIAMGSDYPFPLGEDVPGTLIKNM
                     NLSDQDKLRLLSGTAKEWLGIV"
BASE COUNT         1337 a          854 c          941 g         1134 t
ORIGIN      
        1 tacaatttcg ttcacacaat gaacaaccgt ggtgttcctg atacattaag aattgatgga
       61 ttaagcacat atcgaattgt tgcccataca ttaccacccg ttgagaaaaa agatgttcag
      121 ttagttgctg gaaaacacac gattattccc attgatgctc ctcaaggttt tttaatcctc
      181 aaagctgggg aaggcatgac ttaccgcgat ttgcagtgta tcattcgtaa agcgggcgat
      241 atgaatacct tggatgttat gtcgttcaaa gacacgcgta agttgattgt tgggaaatat
      301 gatttagaaa tattgaccct tccacgtacc aatttgaaag cagttgatat tgctcaaagt
      361 catacaacaa ctgtcaaaat cgacgagcca ggaacggtga actttcagtt gccattacaa
      421 ggaattgcta gtgttttatt ggaagataaa ggacaaatga tttgggtgtg taatctgagt
      481 gaaaacacgc aacaggaagt agtacgatta caacccggaa aatacaaagc ggtatttcgc
      541 ggtaaaggcg tgagagaaac gctattcact attgagaaaa acttcatcgt tcaaccagga
      601 acctcgcaac aggtcgtttt aaaataatgc ttatggaacc atttacattt acggatcaaa
      661 atgatacacg ctctggattt ggagctggat tgcacgaatt aggaaaaagc aatccgaatg
      721 ttgttgcgct gtgtgccgat ttaattggtt cattgaaaat ggatgcattt gtgaaggatt
      781 tcccggagcg ttttttccaa gcaggaattg ccgaagccaa tatgattggt atggcagctg
      841 gattaaccat tggaggaaag attcctttta ccggaacttt tgctaatttc tctacaggtc
      901 gcgtgtatga tcaaattcgt cagagtgttg cctattctgg gaagaatgta aaaatttgtg
      961 cttctcatgc cggtttaaca cttggtgaag atggcgctac tcatcaaata ttggaagata
     1021 ttggtttgat gaaaatgttg ccacacatgg tggtaataaa tccttgcgat tacaatcaaa
     1081 cgaaagctgc aaccatcgcc attgctgatt atgatggtcc ggtttattta cgctttggtc
     1141 gtccgaaagt gccgaacttc actccagcag atcaaaagtt tgagattgga aaagcggtga
     1201 ttttaaagga aggaaaagac gtaagtattt ttgcaacagg ccatttggtt tggaaagcat
     1261 taaaagctgc agaagcattg tgggagaagg gaatcgatgc agaagtaata aatattcata
     1321 cgattaaacc actcgatgat gaagcagtca ttgcctcggt cggtaagacc ggatgcgcag
     1381 tttcggccga ggaacatcag cggaatggag gtttaggaga tagcatagca caattgctag
     1441 cacgtacttt accacgaccg ctagaaatgg tggctgtaaa cgacagtttt ggagaaagcg
     1501 ggacgccaga agaattgatg gtgaaatacg gtttagatac tgtaaatatc atcgaggcag
     1561 ctgagtcagc tattcgccga cgctaaacca accggaaaac cagaccggag cgaacgtatg
     1621 gaagaattca gcgaccggga gctattggat ttatttgcac aagcatcaac gcgaaatcat
     1681 gcgttcaata tgctcgtgcg ccaatatcag caacggttgt actggcatat ccgcaaaatg
     1741 gtactagatc atgacgatgc ggatgatttg ctccaaaatg tatttatcaa agcatttaag
     1801 gccttagata attttcggga ggattcgaaa atctatactt ggctttataa gattgcaacc
     1861 aatgagtgca tcacgtttct gaatactaag aagaagcgat ttatggtgcc catggcggat
     1921 gtggaatatc agctatctca gaatttagaa acggatagtt atttcagtgg cgaccgcatt
     1981 caaatgaaat tacaacaagc tattctgacg ctacctgaca agcagcgact ggttttcaat
     2041 ttgaaatatt tcgaagaaat gaaatatgag gatatgtcgg agattttagg gacatccgtt
     2101 ggtgctctga aagcatcctt tcaccatgct gttaagaaga ttgaagaatt tatgaaacgc
     2161 gattaaacca agttgaatca aacgaatcac aggcaatcat gaacccagaa gaaacacccg
     2221 attttattcc agaaggcttc ccgaagggca atccatttcg ggttccggaa ggatacttcg
     2281 atcgtttccc ggctaagctg gcagaacgga tggaagaaga caagaaaatt cggcgctttc
     2341 catttccttc actactaaaa cctgcaccat tactagctgc agcgtgtatg ctaattatag
     2401 caggtgtgtt tggttataga atgttatccg gtccagctga tccactaacc gatgatgaga
     2461 tttcgaccta tgtgtatcaa gaaggaatta ttgatgaatt ggaagtggac gatatcttag
     2521 aaagtaccga tatggccgaa tcgacagata ccactccaac aagagcatat acaaacgaaa
     2581 acgaagaaat tcaatattat ctgttagatg aggacatcga cgatgcagat atcattaacg
     2641 aactttagaa accatcacat gaaactccaa ttgataatcc tgttgttaat cctgaacttt
     2701 ccaggaattg cacaaaaaga taatgttcgt gagcgaatca agtcgcaacg catcgcattt
     2761 atcactcaaa aactagcttt gacgcccgat gaagcacaaa aattctggcc ggtttacaat
     2821 cagtattccg acgaaattga tcgagttaaa aaggatatga ataagttacg tcgttccaca
     2881 aacgacaatc tgatgagcat gaatgataag gatattgaaa aagccttgga agacgacctt
     2941 cagaaccagc agaaaatcac tgacttgcaa cgtaagtatc aatcggaact caagaaagta
     3001 attcctgcta gaaaaattgc tcaactgtat aaagcagaac gggatttcaa actcaccctc
     3061 ttaaaacgca tgcgtgattc tggacatcaa ggtccgccgc cagaggaaga attgtaaatt
     3121 aaaatcacaa ccatcacagc taaaccgtct cttacgtatc gacctccttc gatgcgacaa
     3181 gaggcggttt tattttatac tataccaagc cattctttag ccgttccgct taataagcgt
     3241 aatttatctt gatcacttaa attcatgttt ttgatgagcg taccaggcac atcttcacct
     3301 agagggaaag gataatcaga gcccattgca atgcgttcca ctcctgcaaa atcgattaaa
     3361 taacgcatag ctgcttcatc gtgaacaagt ccgtcgaagt ataaacgatt gaggtatttt
     3421 tcgggactaa cagcattgtc gattgcaact aaatcggggc gacaatcgaa gccatgttta
     3481 attcgaccaa gtgtaagtgg aaacgagcca ccaccatgtg caaaagctac acgtaacttg
     3541 ggcaaacgct cgaaaattcc accgaaaatc atggaacaaa ttgcacgaga agtttcggcg
     3601 ggcattccaa ctaaccatgg taaccaataa cgttggatat ctttttggcc aaacatttcc
     3661 caaggatgaa caaatatggc agcgcctaat tcttcagcag cttggaatat ttcaaagaat
     3721 tcgggttcat tcaaattcaa ttgattgacg ttgctcccaa tttgaattcc agccattcct
     3781 aactctttca tgcaacgttc caactcacga atagccagtt tgggcgattg catcgggata
     3841 gttcctaatc caataaaacg atctggataa tcgcgaacaa taccagcaat atgatcattg
     3901 agaaattccg aaacagcgag cgcatcttcg ccttttgccc agtatgaaaa catcaccgga
     3961 accgtgctca aaacctgcat gtgcacacca tgatgctcac attcgtgcat tcttgccttc
     4021 ggatcccagc aattgtcctg cacttcccga aaaaactgat catccttcat catccgcgca
     4081 caacaaggct tgtgatgatc gagatgaata aatccaccat acccaaatcg ctccttgaaa
     4141 tttggaatgt gttcaggaag aatgtgggtg tggatgtcga tggttaacat ggctggtgtg
     4201 ctggtgtgct ggtgtgctgg tgtgctggtg tgctggtgtg ctggtgtgct tgtgtgctgg
     4261 tgtgct
//
LOCUS       DAOOQW010000210         4253 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_24677_length_4253_cov_4.891377, whole genome shotgun sequence.
ACCESSION   DAOOQW010000210 DAOOQW010000000
VERSION     DAOOQW010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4253
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24677_length_4253_cov_4.891377"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1078)
                     /locus_tag="PK281_10045"
     CDS             complement(<1..1078)
                     /locus_tag="PK281_10045"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HOY49724.1"
                     /translation="MKRLLAYIRPVHHYLPEYIFYTFLAIIFGIVNFTMLIPLLNLLF
                     STDGIPETVVQPTFSLSISYFSDLFNYYLGSFAAEKGKLYAMAFVCIVIASATLLANI
                     FRYLSTRVLVRLKLNMLQRLRNHLYEKVSGQSMVWFNKNRKGDVISTMTNDVQEIEFS
                     VISSFQTLLRDPFVIIAYFITLFYISPALTFFTILFFPISGLIIATLAKQLKKKGYFS
                     QEMLGRILQHTDETISGIRVIQSFRAIGVFRERFQNINSQFTSNSKALFNQRELASPL
                     SELLGVIVVVVVVMYGGNLVLKNELTGSLFITYLALYSQILQPAKNITSAISNMQKGF
                     VSAERIFAILDESDAILDVNQAKEI"
     gene            1086..1913
                     /locus_tag="PK281_10050"
     CDS             1086..1913
                     /locus_tag="PK281_10050"
                     /inference="COORDINATES: protein motif:HMM:NF013882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HOY49725.1"
                     /translation="MSIQEWVELGGKLQISTTFRGMNIQLFKNTNNPQNLEIGVYVLH
                     VKKGYESRAERIQRELSELGISFEYILDWDKNELNEEQLSRFFAGTMHSVSAATSCAS
                     KHIESWSKFSKSSHELALILEDDIIPDKHFLAFLTQFVEELIVLNPNLLNPVFISLEN
                     SGLQFVPAHQLEKNKFLYPVTDTRCAGAYLINRSASELLLHTLESYKMDEPVDWWLKR
                     LAEKKILHVLWSHPTIAEQGSHNGHYASSIDHKTEGVFRKFSWILKRFYKNTLNSFR"
     gene            1972..2322
                     /gene="rbfA"
                     /locus_tag="PK281_10055"
     CDS             1972..2322
                     /gene="rbfA"
                     /locus_tag="PK281_10055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOY49726.1"
                     /translation="MTHTESTRQKKVARLIQKEMAEFFLHESRNLFNGAFITVTTSRI
                     SPDLGYAKIYLSMFKVADQQALLGQINLIKKEIRKKLGEKVRHQLRIVPELEFYIDDS
                     LDYLENIDRLLKND"
     gene            2326..3552
                     /locus_tag="PK281_10060"
     CDS             2326..3552
                     /locus_tag="PK281_10060"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOY49727.1"
                     /translation="MRFPLFIALRYLFSKKSTHVINIISGISVTGILVGTCALVILLS
                     AFNGLESWVVNLYNSFDPDIRVEAKSRKFFQETELPRTKILEISGVERVMQVIEENGL
                     LTHRDAQYVCTIKGVSDELVKMSGLDTMMVEGDFRLNRGQIPMAVVGSGIAYALSLSL
                     SDLESPLDIYVPKANASFSLNPAEAFHSSSIRTAGIFEVQPEFDLKYIIVPIGFAREL
                     FHRETELSSYEIQLKPDADLDQVMKSLKALTGEGFAIKNRFQQHELLYKIINAEKWGV
                     FLILAFILLIAIFNVTGSLTMLIVDKSRDIRTLQSLGASWSVIRLIFFLEGMMISFLG
                     MSIGIVTGLVVVYLQSKFNLVLINGEDAYPVLVKGADILYISLTVLAIGALASWLPAS
                     RTLTKRRLSQLGQADR"
     gene            complement(3553..>4253)
                     /locus_tag="PK281_10065"
     CDS             complement(3553..>4253)
                     /locus_tag="PK281_10065"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOY49728.1"
                     /translation="KDIDNKKLMGQYQLTQEELQKKEDRLRDAEADLEKRKAEVEKLS
                     ADMRIYEEELNNKEKRVAELESVLAKKDSTVSALKEKVSAALLGFQDNGLSVSTRNGK
                     VYVSLEERLLFESGSTVVDAKGVDALKKLAKVLERETDINVLIEGHTDNVPIKSASIK
                     DNWDLSVLRATSIVRIITANSKVDPRSLTAAGRGEYFPIDKSNTPEARRKNRRTEIIL
                     TPNLDELFKILETN"
BASE COUNT         1327 a          739 c          886 g         1301 t
ORIGIN      
        1 gaatttcctt agcctgattc acatccaaaa tagcatccga ttcatctaaa atggcaaaga
       61 ttcgttcggc agaaacaaag cctttctgca tgtttgaaat ggctgaggtt atgtttttag
      121 ccggttgtaa aatctgtgag taaagagcta gataggtaat gaaaagtgat ccagttagtt
      181 catttttcaa gaccaaatta ccaccgtaca tgactaccac aacaactaca attactccga
      241 gtagttcgga taaaggagat gcgagctccc gttgattgaa tagcgcttta ctgttagatg
      301 taaattggga attaatgttt tgaaatcgtt cccgaaaaac accaattgca cgaaatgatt
      361 gaataacacg aatacctgaa atggtttcat ctgtatgttg caatatacga ccgagcattt
      421 cttggctgaa gtaccctttt ttcttgagtt gtttggctaa tgtggctatg attaatccag
      481 aaatcgggaa aaataaaatg gtaaaaaatg tgagagcagg tgaaatgtaa aaaagggtaa
      541 tgaaatatgc gatgataacg aatggatcac gtaaaagcgt ttgaaaagaa ctgataactg
      601 aaaattcaat ttcttgaaca tcgttggtca tggtagagat tacatcacct tttctattct
      661 tattaaacca aaccatggat tgaccggaaa ccttttcata gaggtgattt cgcaatcgtt
      721 ggagcatatt tagtttaaga cgaactagca ctcgtgtact taaatagcgg aaaatattag
      781 cgagtaaggt agctgaggca attacgatac aaacaaatgc cattgcgtag agtttacctt
      841 tctcggcggc aaaactccct aggtaataat taaataaatc actgaagtag ctaatactta
      901 atgaaaatgt aggctgaaca actgtttcag gaataccatc agtgctgaat agaagattca
      961 acaagggtat tagcatggta aaattgacga taccgaaaat gatcgccaag aaggtgtaaa
     1021 aaatatattc cggaagataa tggtgtaccg gacgaatgta tgccagcagg cgtttcaaat
     1081 ggtacatgag tattcaggaa tgggttgaat tgggcggcaa attacaaatt tctactacct
     1141 ttcgcgggat gaacattcaa ctgtttaaga acaccaataa tcctcaaaac ttagaaatag
     1201 gtgtttatgt tttacatgta aagaaaggct atgaatcgcg tgccgaaagg attcaacgcg
     1261 aattatcaga gttgggtatt tcgttcgaat atattttgga ctgggacaaa aatgagttga
     1321 atgaagagca attgagtcgc ttttttgcag gcactatgca tagtgtttcg gctgctacat
     1381 cctgtgcttc aaaacatatt gaatcctgga gtaaattttc aaagtcatca catgaacttg
     1441 ctttaatttt agaggatgac ataattccgg acaagcactt tttagctttc ttaactcaat
     1501 tcgttgaaga attaatcgtg ttaaatccca atcttttaaa tcctgttttt atatcattgg
     1561 aaaattccgg attacaattc gtacccgcac atcaacttga aaaaaacaag ttcttatatc
     1621 ctgtaaccga tacgcgatgt gcaggtgcat atctaataaa ccgatctgct tctgaattgt
     1681 tgcttcacac gctggaatca tataaaatgg atgaacctgt ggattggtgg ctcaagcgac
     1741 tggctgaaaa gaaaatatta catgttttat ggagtcatcc aacaatagct gaacaaggaa
     1801 gtcataatgg acactatgca tcttcgattg accataaaac cgaaggagta tttcgaaagt
     1861 tcagttggat acttaaacga ttttataaga acaccttaaa cagctttcga taatcgagca
     1921 taagcggaat taaaataata gctttgcttt tttaaaagaa ggcatcaaaa gatgacacat
     1981 acagaatcga cacgtcagaa aaaagtagct cgtttaattc aaaaggagat ggctgaattc
     2041 tttctgcacg aaagccgaaa cctgtttaat ggagcattca ttacagtaac aacttcacga
     2101 attagtcctg atttggggta tgcaaaaata tacctgagta tgtttaaagt tgctgatcag
     2161 caggctcttt tgggacaaat caatttgatt aaaaaagaaa tccgtaagaa gttaggagaa
     2221 aaagttcggc atcaattacg aattgttcct gagctagaat tttacataga tgattcgttg
     2281 gattatctag agaatattga ccgattgctg aaaaacgatt aagctttgcg tttccctctg
     2341 tttatcgctc tgcgttatct tttctcgaaa aagagcacac atgtgattaa catcatttcg
     2401 ggtatctcgg ttacgggtat tctcgtggga acgtgtgcat tagtaatttt actgtcggca
     2461 ttcaatggat tggaaagttg ggttgtaaac ctgtataaca gctttgatcc ggacattcgc
     2521 gttgaagcta agagtcgtaa atttttccaa gagaccgaat taccacgaac taaaatacta
     2581 gagatttctg gtgttgaacg agttatgcag gtgattgaag agaatggctt acttacacat
     2641 cgagatgctc aatatgtatg tacgataaaa ggtgtgagtg acgagctcgt gaaaatgagt
     2701 gggttggata cgatgatggt tgagggagat ttcagactaa atcgcggaca aattccaatg
     2761 gcagtagtag gaagcggaat tgcctatgcg ctttcgttaa gcttaagtga tttggaatca
     2821 ccactcgata tttatgttcc caaggcaaat gcgagcttta gtttgaaccc agctgaagcg
     2881 tttcatagta gtagtattcg cacggcgggg atatttgagg tgcagccaga gtttgacctc
     2941 aaatacatta tcgttccgat aggttttgca cgggaattat ttcatcgaga aacggaactc
     3001 agttcgtatg agatacaatt aaaacctgac gctgatttag atcaggtgat gaaatcatta
     3061 aaagctttga ccggtgaagg attcgcgata aaaaatcgtt ttcagcagca cgaattactt
     3121 tacaagatta tcaatgcaga aaaatggggt gtgtttctga ttttggcatt tatccttttg
     3181 atagccattt tcaatgtaac aggatcattg actatgttga tagtagacaa atccagagat
     3241 attcgaaccc ttcaaagttt aggtgcgagt tggagtgtga ttcgattaat attcttctta
     3301 gaaggaatga tgatttcctt tttagggatg agtattggaa tcgtaactgg tctggtggta
     3361 gtttacttac aaagcaagtt caacctagtt ttgattaacg gcgaagatgc ttatcccgtt
     3421 ttagtaaaag gagctgatat tctttacata tcccttacag tgctagcgat tggtgcacta
     3481 gcatcttggt tgcctgcttc aagaacatta acgaaacgtc gattgtcgca attaggacaa
     3541 gcagatcgtt gattaattcg tttcgagaat tttaaataat tcgtcgaggt tgggagttag
     3601 aattatttct gtccgacgat ttttacgacg cgcttctgga gtgttggatt tgtcgattgg
     3661 gaaatattca ccacgtccag cagcagtaag tgaacgagga tcaaccttcg agttagcagt
     3721 aataattcga acgattgaag tggcacgtaa cacactcaaa tcccaattgt ctttaatact
     3781 ggcacttttg attggaacat tatcggtgtg cccttcgatt aggacgttta tatctgtttc
     3841 acgctcaagc acttttgcca gttttttaag tgcatcaaca ccttttgcgt caacaacagt
     3901 actgcctgat tcgaagagta atctttcttc aagcgacaca tacactttcc cattacgagt
     3961 agaaacggaa agtccattat cttgaaaacc cagtaacgca gctgaaactt tctctttcaa
     4021 agcagaaact gttgaatcct ttttcgccaa aaccgattcc aattcagcaa cacgcttctc
     4081 tttgttattg agttcttctt cgtagatacg catatcagca ctgagttttt ctacttcagc
     4141 tttgcgtttt tctagatctg cttctgcatc gcgtaaacga tcttctttct tttgtaattc
     4201 ttcttgggta agttggtact gacccattaa cttcttgtta tcgatgtctt tac
//
LOCUS       DAOOQW010000211         4155 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_25445_length_4155_cov_4.759024, whole genome shotgun sequence.
ACCESSION   DAOOQW010000211 DAOOQW010000000
VERSION     DAOOQW010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4155
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25445_length_4155_cov_4.759024"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3404)
                     /locus_tag="PK281_10070"
     CDS             complement(<1..3404)
                     /locus_tag="PK281_10070"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HOY49729.1"
                     /translation="MSQSPLLFFRTFLLIVLFQLVACTGSYPKWIEVSPDFIPYVSAY
                     SGGIISSTSTIRVRLAQEIRGIVAFGEPVEEEYFDFEPSISGKTRWVDASTIEFIPEK
                     RLPQNKQYTAHFNLGKLYKVPEKLEDMAFSFKTIQQSAEISLGFPNTYNPDDLSRQFV
                     EGSILTADKADLETVKKTLQARQNSGELTINWSQSADQRKHYFKIEQIKRPLQTADVL
                     QVHYDGAAFDSDETNEWARDLVPQGKFQVVNATVVNDDEPRIELIFSDPLLEMQDLTG
                     LIQVAGTDLPDAVINGHVLSIYQADAVEGTTNLIVSPGILNALGKRLEVGGNYAVTFE
                     DAKPDIRFPGGGVILPGEDGMIVPFEAVSLKKADVQITQIFENNIPQFLQTNNMNGDG
                     GLKQVGKIIAIKTIPLETAHISSRKKYSAFALNLNELIKTEPGAIYRIELRFRKEYAT
                     YVCQDGNESDDMQTVSAANDFKSLNKPGYYGGEYEQEYYGDEGYEEDYDWKERNNPCS
                     STYYLNHQAATRNVLASNIGLIAKNAGDGSWQFIASDIVSAQPLSGVALRVIDFQHQQ
                     IASIKTDSKGFAYLKSSPDDAPFLLIASQGKQKGYLKLNDGEALSTSQFDVSGEEINE
                     GIKGFLYAERGVWRPGDTLFVQFMLETPKDALSGAHPIVFEMTNSRGQGVKKEVQVYD
                     PSQVIYSFPVATGYDSPTGNYTASVTIGSSVFRKNFKVETIMPNRLKIDFNFPDPTAL
                     RASQASSNVPLRVSWLHGAPATSLRANVAVTLSPSSTSFPAFKDYTFDDPSRTISTEE
                     VSVFDGLLDASGEATVNPMIRSTQQAPGQLKADFTVRVFEPGGGFSIDRFSTPYLPYT
                     SFAGVQAPKGEGWGNILTVNKQHTLNLACVNDLGKGIPGRTLSVNFYKTSYEWWWQRN
                     GNINDYYNAEYAVAVKTAKVVTNAAGKAVYQFSFNEDELATYLIRVCDETSGHCTGTT
                     IYVDDDSWMSRNTGKTQHARDLSLQSDKEKYTTGESINISFPSPSHARALVSIEDGSH
                     ILESRWIETEAGKTEFTIKALPEYAPNVYVHLTVLQPYKHANELPVRMYGLLPVFVED
                     PDTRLQPVISAPDVVRPETKLSVGIKEKSGKEMEYTLIPTDSFVSGL"
     gene            3542..3973
                     /locus_tag="PK281_10075"
     CDS             3542..3973
                     /locus_tag="PK281_10075"
                     /inference="COORDINATES: protein motif:HMM:NF022769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3127 domain-containing protein"
                     /protein_id="HOY49730.1"
                     /translation="MALEINGKLVKVMEQVTGESRNGGWVKQEFVIETFDQYPKKVCC
                     SVWGDKTEQLRRFQPGDDVKVSFNVESREYNERWYTEIRAWKLDPAGAVAPAQGQGGA
                     PAYAAPQQNTGSAPAFNQGFQTASAPASPFDNNNESDDLPF"
BASE COUNT         1172 a          845 c          876 g         1262 t
ORIGIN      
        1 agaccggaaa cgaaactatc agttggtatc aaggtatatt ccatttcctt accgctcttt
       61 tccttgatac caactgatag tttcgtttcc ggtcttacca catcaggagc agaaataacg
      121 ggttgcaatc gtgtatcagg atcttccaca aaaacgggaa gcaagccata cattcgcaca
      181 ggaagctcat ttgcgtgttt atagggttgt aatacagtca agtgaacata cacgttaggt
      241 gcatattcgg gtaatgcttt tatggtgaat tcggtttttc ctgcttcggt ttcaatccag
      301 cggctttcta atatgtgcga tccatcttca atactaacta aagcacgagc atgtgacgga
      361 cttgggaagc tgatatttat ggattcgcca gttgtgtatt tttctttgtc cgattgtaac
      421 gaaaggtcgc gtgcatgttg agttttaccc gtgttacggc tcatccagga atcatcatct
      481 acatagattg ttgttccagt gcaatgtcca ctggtttcat cgcaaacacg aataagatag
      541 gtcgctagct catcctcgtt aaaagaaaat tgataaactg ctttaccggc tgcattggta
      601 acaactttgg ccgttttaac agcaacggca tattcagcat tgtagtagtc attgatatta
      661 ccgtttcgct gccaccacca ttcataagat gttttataga agtttacact caaggttctt
      721 ccgggaattc ctttacctaa atcgtttacg catgcaagat ttaaggtatg ctgcttattc
      781 accgtgagaa tatttcccca tccttctcct ttaggtgctt gaactcctgc aaaactcgta
      841 taaggcaaat aaggagtgct gaagcgatcg atactaaacc caccacctgg ttcgaaaact
      901 cgcactgtga aatcagcttt tagttgtccg ggtgcttgtt gagtgctgcg aatcatagga
      961 ttaactgtcg cttcaccaga tgcatcgagc aaaccatcga aaacactgac ttcttcggta
     1021 gaaattgtac gagatggatc atcaaacgta taatccttaa atgctgggaa tgaagtgctt
     1081 gaaggactta atgttacagc aacattggca cgaagtgatg tagccggagc tccatgcagc
     1141 caactaactc gaagtggaac attgctactt gcttgagatg cgcgtaacgc agtaggatcg
     1201 gggaagttga agtcgatttt caaacgattc ggcatgatcg tctccacttt gaaattcttc
     1261 cggaaaacgc tgctacctat ggtgacggaa gcagtgtaat ttcctgtcgg agaatcatat
     1321 ccggtagcta cagggaaact ataaatcact tggcttggat catacacttg tacttctttt
     1381 ttaactcctt gtccacgtga attggtcatt tcaaatacaa tcggatgtgc tccagaaagc
     1441 gcatctttgg gtgtttcgag cataaactga acgaatagcg tatctcctgg tcgccacaca
     1501 ccacgctctg catagagaaa tcctttaatt ccttcgttta tttcttcacc gctcacatca
     1561 aattgactgg tcgataatgc ttcaccatcg tttaatttga gataaccttt ctgtttgcct
     1621 tgagatgcta ttagtaggaa tggagcatcg tctggactac tctttaaata agcaaatcct
     1681 ttactatcgg tttttatgga agcaatttgt tgatgttgaa aatcgatgac acgcaaagca
     1741 actcctgaaa gtggttgagc cgaaacaata tcgcttgcaa tgaactgcca agagccatct
     1801 ccagcatttt tagcaataag accgatatta gaagctaaca catttcgcgt agcagcttgg
     1861 tgatttaaat aataggttga tgaacacgga ttgttacgtt ctttccaatc ataatcttct
     1921 tcgtatcctt cgtcgccgta gtattcttgc tcatattcac ctccataata acccggttta
     1981 ttgagcgatt tgaaatcgtt tgcagcacta acggtttgca tatcatccga ttcgtttcca
     2041 tcctgacaca cataggttgc atattctttt ctaaagcgaa gttcaatccg ataaatagcg
     2101 cccggttcgg ttttaattaa ttcgttcaga tttagggcaa atgcactgta tttctttctg
     2161 cttgaaatat gagccgtttc gagcggaatg gttttgatag cgatgatttt acctacctgt
     2221 ttcaatccgc catcaccgtt catattattg gtttgaagga attgaggaat gttgttttcg
     2281 aagatttgag taatctgaac gtccgctttt ttgagcgata cagcttcaaa tggaacaatc
     2341 atcccatctt cacccggtaa aataactcct ccgccaggaa agcgaatatc tggtttagca
     2401 tcttcaaaag tgacagcgta atttccacca acttcaagtc gttttccaag tgcattcaag
     2461 attcccggtg aaacgattaa gttagttgtc ccttctactg catctgcttg gtaaatagat
     2521 aaaacatgcc cattaattac tgcatcggga aggtctgttc cagcaacttg aatcaaacca
     2581 gttaaatcct gcatttcaag aagcggatcg ctgaaaatca attcaatgcg aggctcatca
     2641 tcattcacga cggtggcatt tactacttgg aattttcctt gaggaacaag atcacgcgcc
     2701 cattcattgg tttcatctga atcgaaagct gctccatcgt aatgaacttg aaggacatcg
     2761 gcggtttgaa gcggtcgttt aatttgttca atcttaaaat aatgcttgcg ttgatcggcg
     2821 ctttggctcc agttaatggt gagttctcct gaattttgtc gagcttgtaa ggtctttttt
     2881 actgtttcta aatcggcttt atcggcggtc aggattgagc cttcaacgaa ttgtcgactt
     2941 aaatcatctg gattataagt attaggaaaa cctaaagaaa tttcggctga ttgttgaatg
     3001 gttttgaacg aaaaggccat gtcttcgagc ttttcgggaa ccttataaag tttacctaaa
     3061 ttgaaatgtg ctgtgtattg tttgttttga ggaagacgtt tttcgggaat gaactcgatt
     3121 gtactagcat ctacccaccg cgttttacct gaaatggaag gttcgaaatc gaaatattct
     3181 tcttcaactg gttctccaaa agcaacaatg ccgcgtattt cctgcgctaa tcgaacgcga
     3241 atagtgcttg ttgatgagat aatccctccc gaataggcac tgacgtaggg aataaaatcg
     3301 ggactcactt caatccactt gggataagag ccggtacagg ctactaattg gaataggacg
     3361 attaataaga atgtcctaaa aaataggaga ggagattggc tcatgcgtgt aaagtagaaa
     3421 gcacgaattt acgggctagg aggaatcaca gaagctttcc acaggaatgt tcaaaactat
     3481 ttaatcgaca gaaggcggtt aggccatttc gattggctaa ttttgagcaa aatttcatgt
     3541 tatggctttg gaaattaatg gtaagcttgt taaggtaatg gaacaagtta ctggcgaatc
     3601 acgcaatggt ggttgggtaa aacaagaatt tgtgatcgag acatttgatc aataccctaa
     3661 aaaagtatgt tgctctgttt ggggtgataa aaccgaacaa cttcgtcgct ttcaaccggg
     3721 tgatgatgtt aaggttagct ttaatgttga atctcgtgaa tataacgagc gttggtacac
     3781 cgaaattcgt gcttggaaat tagatccagc tggtgctgta gctcctgcac aaggtcaagg
     3841 tggcgctcct gcttatgctg caccacagca aaatacaggt tctgcacctg cttttaatca
     3901 aggttttcaa acagctagtg caccagcatc acctttcgat aataacaacg aaagcgacga
     3961 tcttccattc taatcgaata gtagtatgaa gcattcctta agtttcttaa aagaacttgc
     4021 aaagaacaac actcgggaat ggtttgatat tcatcgtaaa gattacgagc aatcaaaaaa
     4081 agaattaagt gatctgactg gagcagttat tcagcagatc gctttgttcg attcaagtat
     4141 tggggcttta agtcc
//
LOCUS       DAOOQW010000212         4148 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_25507_length_4148_cov_3.400195, whole genome shotgun sequence.
ACCESSION   DAOOQW010000212 DAOOQW010000000
VERSION     DAOOQW010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4148
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25507_length_4148_cov_3.400195"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1067
                     /locus_tag="PK281_10080"
     CDS             <1..1067
                     /locus_tag="PK281_10080"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOY49731.1"
                     /translation="PYVEYYLRHPSYQNYPVVGVNWLQANDYCAWRTDRVNESILIRE
                     GVLKMDAVGQVADNNFNTDAYLAGQYEGLVKNDPVDLDPSKGEGATRKVKMEDGILLP
                     RYRLPTEAEWEFAAIGLIGNTTEELITERRLYPWNGHIVRNADDSFKGEMMANFKRGR
                     GDNMGTAGKLNDNADITGEVTAYWPNDYGLYNMAGNVSEWVMDVYRPLTPEDVSDFRA
                     FRGNVYKTVVRNEDKTIADKDSLGRVKFRNVTEEESADRRNYRKSDNINYQDGDRESS
                     TSYGSEDAEEDKIMYEFGATSMINNRSRVYKGGSWKDRAYYLSPGTRRYLDERLSTDY
                     IGFRCAMTRVGSPVGLGKKK"
     gene            1125..2429
                     /gene="murF"
                     /locus_tag="PK281_10085"
     CDS             1125..2429
                     /gene="murF"
                     /locus_tag="PK281_10085"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HOY49732.1"
                     /translation="MIYSIEQLYSVFQKHPSVCTDTRKLIEGSIFFALSGPSFNGNTF
                     ASQAIEGGCSYAVIDDPAYQNGEQYLLVENTLDSLQKLATYHRRQLKTQIIGITGSNG
                     KTTTKELVRNVLAMKYKTLATFGNLNNHIGVPLTLLKIDSSIEMAVIEMGASKQGDIK
                     ELVDIAEPEFGLITNIGLAHLEGMGGPEGVIKTKTELYDYLRIHNGLVFVNSHHTVFE
                     EKSMGINQFRFGAKETDDVKGKFEGSHPYVSFKWTSQKDWSNQPLIETQLMGRYNYEN
                     ILAAVAIGVYFDVNEQDINDAIRSYSPDNNRSQLIETGKNTLIMDAYNANPTSLEAAL
                     LNFAAMEKVNKVVIIGKMMELGPTTLEQHAKIGNLAVTSGFDQVYLIGENYQISGVQG
                     ATQLFNNTEEAINWFSMHPLIDSTILIKGSRANQLEKLQPLF"
     gene            complement(2483..3622)
                     /locus_tag="PK281_10090"
     CDS             complement(2483..3622)
                     /locus_tag="PK281_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019037619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HOY49733.1"
                     /translation="MHIQLSEEHLMIQKAARDFAQNELKPGVIERDEKQQFPAEQIKM
                     LGELGFMGMMVDPKYGGSGMDTISYVLVMEELSKIDASVSVAVSVNNSLVCWGLETFG
                     SEEQKLKYLVPLAKGEKLGAFCLSEPEAGSDATSQQTTAEDKGDYYLLNGTKNWITNG
                     SSASTYIVIAQTDPSKGSRGINAFIVERGMEGFVVGAKENKLGIRGSDTHTLLFNDVK
                     VPKENRIGEDGFGFKFAMKTLSGGRIGIAAQALGIASGAYELALAYSKQRSAFGKVIS
                     QHQAIQFKLADMATEIEAARLLVFKAAWEKDQHLDYTTSGAMAKLYASRVAMETTVEA
                     VQIHGGYGFVKEYHVERLMRDAKITQIYEGTSEVQKIVIARSVLS"
     gene            3679..>4148
                     /locus_tag="PK281_10095"
     CDS             3679..>4148
                     /locus_tag="PK281_10095"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF015647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HOY49734.1"
                     /translation="MEVLSDVLTGIGVMSGTSIDGLDLIAVTFYKSNNKICYDVSASE
                     TIPYSSTWKNSFLHATELSAVGLRKLDVEFAHFISKSVLEFIKEKNWKPDFISSHGHT
                     IFHQPNSGYTHQIGCGSTIAALTGIQTVCDFRQGDVALGGQGAPLVPIGDQLLFS"
BASE COUNT         1318 a          741 c          802 g         1287 t
ORIGIN      
        1 agccttatgt tgaatattat ttacgtcacc cttcatatca aaactatcct gttgttgggg
       61 taaattggct tcaagcgaac gactattgtg cttggagaac tgaccgagtt aacgaatcga
      121 ttttgattcg tgagggtgtt ttgaaaatgg acgcggtagg tcaagttgcc gataacaact
      181 tcaatacaga tgcatactta gcaggacaat acgaaggtct tgtgaaaaac gatccagttg
      241 atcttgatcc aagtaaaggt gaaggagcaa ctcgtaaagt aaaaatggaa gatggtattc
      301 ttcttcctcg ttacagactc ccaacagaag cagaatggga atttgctgct atcggtttga
      361 tcggtaatac tactgaagaa ttaatcactg aacgtcgttt gtatccttgg aatggacata
      421 ttgttcgtaa tgcggatgat agtttcaaag gagaaatgat ggctaacttt aaacgtggtc
      481 gaggtgataa catgggtact gccggtaaat tgaatgataa tgccgacatt actggtgagg
      541 ttactgctta ctggccaaat gattatggtt tgtataacat ggctggaaac gtttctgaat
      601 gggttatgga tgtgtatcgt ccattaacac cagaagatgt atctgatttc cgtgctttcc
      661 gtggtaacgt gtacaaaacg gttgtacgta atgaggataa aacaatagcg gataaagatt
      721 cactaggtcg agttaaattc cgtaatgtaa cggaggaaga aagtgcggat cgtagaaact
      781 atcgtaaatc agataacatt aattatcaag atggtgatcg cgaatcaagt acttcatatg
      841 gttcagaaga tgctgaagaa gacaaaatca tgtatgaatt tggggctact tcaatgatca
      901 ataatcgttc tcgtgtatac aaaggtggtt cttggaaaga cagagcttat tacctttctc
      961 caggtacacg tcgttactta gatgagcgtc tttctacaga ttatattggg ttccgttgtg
     1021 caatgacacg agtaggaagc cctgttggtt taggaaagaa gaaataatat ttcacttaca
     1081 atttcaaccc cgtaaatttt acatttacgg ggtttctttt ttatatgata tactccatcg
     1141 aacaacttta ttcagtattc caaaaacatc cgagcgtttg cactgatact cggaaattaa
     1201 ttgaaggaag catttttttc gctcttagtg gcccaagttt taatggaaat acattcgcct
     1261 ctcaagccat tgaagggggt tgcagttatg cagtaatcga tgatcccgca tatcaaaatg
     1321 gagaacaata tctgcttgta gaaaatactt tggattcact tcaaaaattg gcaacatacc
     1381 atagaagaca actaaaaact caaataatag gcattactgg tagtaatgga aaaactacaa
     1441 ccaaagaatt agtaaggaat gtccttgcta tgaaatataa aacacttgcc acttttggca
     1501 atttgaataa tcatataggt gttcccctaa cattgctaaa aatagattca agtattgaaa
     1561 tggctgtcat tgaaatgggg gcaagtaaac agggggatat taaagagttg gttgatattg
     1621 ctgaacctga atttggtttg atcaccaata ttggtttagc acatcttgaa ggcatgggtg
     1681 gacctgaagg tgtaattaaa acgaaaaccg agttatatga ttatttgaga attcacaatg
     1741 gtttagtttt cgttaattct catcatacag tgtttgaaga gaaatcaatg ggtatcaacc
     1801 aattccgatt tggtgcaaaa gagactgacg atgttaaagg taagtttgaa ggctcacatc
     1861 cttatgtttc atttaaatgg acttcacaaa aagattggag caatcagcca ctaattgaga
     1921 cgcaattaat gggtcgttat aattatgaaa atatattagc ggcagtagca attggcgttt
     1981 atttcgatgt gaatgagcaa gatattaatg atgcaattcg tagctattcg cccgataata
     2041 atcgttctca gcttattgaa acgggaaaga acacacttat tatggatgca tataatgcca
     2101 atccaacaag tttagaggct gcattactca attttgcagc aatggaaaaa gtaaataagg
     2161 tggtaattat tggaaaaatg atggaattag gtccaactac tttggaacaa catgctaaaa
     2221 ttggaaattt agctgtaaca agtggtttcg accaggtata tctgattgga gaaaactatc
     2281 aaattagcgg tgtacaaggt gctacacaac tattcaataa taccgaagag gctattaatt
     2341 ggtttagtat gcatccattg attgattcaa caatattaat taaaggatca agggctaatc
     2401 aattagaaaa acttcaacct ttgttctaaa taaaaacgcc gacctctaat tgaagtcggc
     2461 gttttatctt atcggacagt tcttagctta gaactgatcg tgcaataaca attttttgca
     2521 cttcagaagt tccttcgtag atctgagtaa tttttgcatc acgcattaaa cgttcaacat
     2581 ggtattcttt aacaaaacca tacccaccat gtatttgaac tgcttcaact gttgtttcca
     2641 tggcaactcg agatgcgtat aattttgcca tcgctcctga agttgtataa tcaagatgtt
     2701 gatctttttc ccatgcagct ttaaatacaa gcaagcgtgc agcttcaatt tcagttgcca
     2761 tatcggcaag tttgaattga atagcttgat gctgagaaat cacttttcca aaagcagaac
     2821 gttgttttga ataagctaat gccaattcat aagctccaga agctattcct aaagcttgag
     2881 cagcaattcc aattcgaccg cctgaaagtg ttttcatagc gaatttaaat ccgaatccat
     2941 cctcccctat tcgattttct tttggaactt ttacatcgtt aaataacaat gtatgagtat
     3001 cagaacctcg gatgccaagt ttattttctt ttgctccaac aacaaaacct tccattcctc
     3061 gctcaacgat aaatgcattg attcctctag agccttttga aggatcggtt tgtgcaatta
     3121 ctatatatgt agaagcagaa cttccgttag taatccaatt tttagttccg ttcaataggt
     3181 agtaatctcc tttatcttcg gcagttgttt gttgcgaagt tgcatctgat ccagcttctg
     3241 gttcagataa acaaaaagca cctaattttt ctcctttagc aagtggaact aaatacttta
     3301 atttttgctc ttcagatcca aatgtttcaa gcccccagca taccaaagag ttgttcaccg
     3361 aaacagcaac tgatacagaa gcatcaattt tagatagttc ttccattacc aaaacatagg
     3421 aaatagtgtc cattccacta ccaccgtatt taggatctac catcatgccc atgaaaccaa
     3481 gttccccaag cattttaatt tgttcagcag ggaattgctg tttttcatca cgttcgataa
     3541 ccccaggttt taactcattc tgcgcgaaat ccctcgcagc tttttggatc atcagatgtt
     3601 cttcggataa ttgtatatgc atcgcttgat gtttggacgc caaaaataac agatattcat
     3661 catttactca cgctaaatat ggaagtatta tcagatgttc ttacaggaat tggagttatg
     3721 tcgggaacat ccattgatgg actcgatcta atcgctgtaa cattctataa atcaaacaat
     3781 aagatttgtt atgatgtttc tgcatctgaa acaattccct attcgagcac ttggaaaaat
     3841 tcttttttac atgcaactga actatctgca gtgggattaa gaaagttaga tgtagaattc
     3901 gctcatttca tttccaaatc agttctcgaa tttattaaag agaaaaactg gaaacctgat
     3961 tttatctctt cgcatggaca tacaatcttc catcaaccta attcaggata tacacatcaa
     4021 attggttgcg gttcaacaat cgcagcatta actggaatac aaactgtatg tgatttcaga
     4081 caaggcgatg ttgcattagg aggacaagga gctccattag ttccaattgg cgatcaattg
     4141 cttttctc
//
LOCUS       DAOOQW010000213         4139 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_25559_length_4139_cov_6.219638, whole genome shotgun sequence.
ACCESSION   DAOOQW010000213 DAOOQW010000000
VERSION     DAOOQW010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4139
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25559_length_4139_cov_6.219638"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..948
                     /locus_tag="PK281_10100"
     CDS             <1..948
                     /locus_tag="PK281_10100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49735.1"
                     /translation="ELENIGILQRRALIGHLFNQIGHNQPGELLSTPMSRSYSIGDQT
                     EVLFITEQLDSTGKFLIQSPTQLIINTITQVTKGEESDEIRFETYVTDVTTGTREFVG
                     NNIMTAYTEGITIGSDLVVADTGQKQLLHILPYSTPVFPHPFYPLQTIEGPSVEAYWN
                     DTSIVENSRIPFNCYYTSLFPARICWNNDLPFVWRDNGEGLQGRIVYLKKGEKVLGNS
                     YSVPTNSKTHIQEIGLLNQKLTLNMFAASKETVSMTTFNNKGEKISSTKLKLNAGNTP
                     FELEMIGIESGQTLRIQIQSNNKKAELIQEYQLTSRSNP"
     gene            963..1997
                     /locus_tag="PK281_10105"
     CDS             963..1997
                     /locus_tag="PK281_10105"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOY49736.1"
                     /translation="MKYKLLGQSGLRVSELCLGTMTFGLEWGWGSDKPESKEVFDVYA
                     NAGGNFVDTANRYTEGTSERWLGEFIAADRDHFVLASKYSLKDKNGDPNFAGNHRKNL
                     IRSVKESLKRLQTSHIDLLWLHAWDFTMAPEEVMRALEDLIRQGDVHHIGISDTPAWI
                     IAQANTIAQFRGWNSFVGLQVEYSLLQRTPEADLLPMAKAFGLALTPWAPLAGGALTG
                     KYLNAEAGRVKEESPRRNERANRIATEVVAIATELGVSPSHVALQWTRQRDQVVIPIV
                     GARKASQLLDNIGVVGFVLPETHFNRLQEVSQIELGFPHEFLATTGVQDVLFGGTYNQ
                     VENHQKSYLR"
     gene            2021..2797
                     /locus_tag="PK281_10110"
     CDS             2021..2797
                     /locus_tag="PK281_10110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49737.1"
                     /translation="MGKNIVLLLCLIYAGDILGQSVYIDRAIAAADHERKRPLAEAIE
                     YGFNGVSAEIELKKDGKLYCGSGTFRERYLEPLLARSNNGQAFIHPEHTDEFLLFLEV
                     KSDSSTTYAALKKEIEPYHAMLTSFEGTRKTKKPVRLVLSGKVPHQVIYSESVRRVFC
                     EDMANKLDQSHDGTYITVASLKMKKLFDWKGEGTMPNMAYHSLGSYMKNAHKLGRWVR
                     VYDLPEIPNAFDIIYEAGADYLEIKDLKSFSEYWKKRKPY"
     gene            2878..>4139
                     /locus_tag="PK281_10115"
     CDS             2878..>4139
                     /locus_tag="PK281_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49738.1"
                     /translation="MLSRVSKTIRIIWKYTRKPLLGLAAVLGIVLISAWLLAAFFEDE
                     VKAYFLQKINTELNTRVEIGEIHLSLLDHFPQASVSLQNVKADHSKPYRGAGKLLEAQ
                     SIDFSFSVWDLFSGSYSIHRIDLSNGAMNILRNSDGIINYQLLKPQEKSSSSDEKFSF
                     ELKSLHVNQMNILIRDEPSSFSTLFLVNSGQFKGAFTDDVFDLDIDTDVQLTHLKSDE
                     TSWLSDRPVSIDVGLKINKSTDLYTFTGGDIQISDLQLTVDGSIQNQSDPVFDLKLGG
                     KDLDISSFLSVLPAGYDEQIKKYRSDGKFYCSAELKGVWGKKTYPLITASFGISEGEI
                     TQKENDIQLEGVNLIGTFTNGSQHNMTSSELSLKQVRMKLRGGEVKGDLLIRNFSQPY
                     IQVKADASLALSDIQQFFPLDPLQLKSGT"
BASE COUNT         1290 a          783 c          864 g         1202 t
ORIGIN      
        1 gaacttgaaa acattggtat tcttcaacgt cgtgccttaa ttggtcattt attcaatcaa
       61 ataggtcata atcagccagg tgaattactt agtacgccaa tgagtagaag ctattcaatt
      121 ggggatcaaa ccgaagtgtt atttattacc gaacaactcg attctaccgg taaattccta
      181 atacaatcac ccactcaact aataatcaat accatcaccc aagttacaaa aggtgaagaa
      241 tcagatgaga ttcggttcga aacgtatgta acggatgtaa ctactggtac tagagaattt
      301 gttggtaaca atatcatgac agcctacaca gaaggcatta cgattggttc tgatttagtg
      361 gtagcagata ccggacaaaa acaacttctt catattctac cttattcaac tcctgtgttt
      421 cctcacccat tttatccttt gcaaacgatt gaaggacctt ctgttgaagc ctattggaat
      481 gatacaagca tcgttgaaaa cagtcgcatc ccatttaatt gctactacac tagtttattt
      541 cctgcacgca tttgttggaa caatgattta ccattcgttt ggcgtgacaa tggtgaaggt
      601 cttcaaggtc gaatagttta cctcaaaaaa ggtgaaaaag tcttagggaa ttcctattca
      661 gttccgacaa attccaaaac tcatatacaa gaaattggac tgcttaatca gaagctaacg
      721 cttaacatgt ttgctgcttc taaggaaact gtatcgatga caacatttaa taataaaggc
      781 gaaaagatta gttcaacaaa attgaaattg aatgctggta atacgccatt tgaattagag
      841 atgataggaa ttgaaagtgg acaaactctt cgtatacaga ttcaatcgaa taataaaaaa
      901 gcagagctca ttcaagagta tcaactaact tcacgatcaa atccttaacg ataaacatta
      961 aaatgaagta taagttattg ggtcagagcg ggcttcgagt ttctgaattg tgtttgggca
     1021 ccatgacatt tggattagaa tggggctggg gatcggataa accagaaagt aaggaagtgt
     1081 ttgatgtata tgcgaatgcg ggtggaaact ttgtcgacac agcaaatcga tatactgaag
     1141 ggacaagtga acggtggttg ggagaattta tcgctgctga tcgagaccat tttgtgttag
     1201 cctctaaata ttccttgaag gataaaaacg gagatccaaa ttttgcagga aatcatcgaa
     1261 aaaatctgat tcgttcggtt aaggaaagtc ttaaacgcct gcaaacttcg cacatagatt
     1321 tgctatggct ccatgcctgg gattttacca tggcacccga agaagttatg cgtgctcttg
     1381 aagatcttat ccgccaggga gatgttcatc atattggaat aagtgatacg cctgcctgga
     1441 ttattgctca agccaacacc attgctcaat ttcgtggatg gaacagcttt gttggtttgc
     1501 aagtagaata cagcttatta caacgtacgc ctgaagccga tttactgccc atggcaaaag
     1561 ctttcggatt agctctaaca ccatgggctc cgcttgctgg tggagcttta acaggtaaat
     1621 accttaatgc cgaagctgga cgcgtaaaag aagaaagtcc acgtcgaaac gaacgtgcta
     1681 accgaattgc caccgaagtt gttgctattg caactgaatt gggagttagt ccatctcatg
     1741 tggcattaca gtggactcgt cagcgtgatc aagtggtgat tcctattgta ggagctagaa
     1801 aagccagtca gttattagat aatattggtg ttgttggttt cgttttaccg gaaacacatt
     1861 ttaatcgatt gcaagaagta agtcagatag aacttggctt tcctcacgaa tttttagcaa
     1921 caacgggtgt tcaggatgta ttgtttgggg gaacatacaa ccaagtagag aaccaccaaa
     1981 aaagctatct acgttaatca attttcaaaa tctactaaac atggggaaga atattgtgct
     2041 gttgttatgt ttgatttatg ccggtgatat cctcggacaa tcggtgtata tagatcgtgc
     2101 aattgctgca gctgatcacg aacgtaaacg tccattagca gaagctatcg aatatggttt
     2161 taatggtgta tcggctgaga tagaactcaa gaaagatggc aaactatact gtggctccgg
     2221 tacattccgc gaacgctatt tagaacctct tttagcacgt tcgaataatg gacaagcatt
     2281 tattcatccc gaacatactg atgagtttct cctatttcta gaagttaaga gtgacagtag
     2341 cacgacctat gcagctttga aaaaggaaat cgaaccttat catgcgatgc ttacttcatt
     2401 tgaaggcaca cgtaaaacta agaaacccgt tcgattggtg ctttcgggaa aagtgccaca
     2461 tcaagtaatt tattccgaat cggtacgacg tgttttttgt gaggacatgg ctaacaaact
     2521 tgaccagtcg cacgatggaa catacattac agttgctagc ttaaagatga agaagctatt
     2581 cgattggaaa ggtgaaggaa caatgcccaa catggcgtat cacagtttgg gttcgtacat
     2641 gaaaaatgca cataaattgg gtcgttgggt acgtgtttac gatttgcccg aaattcctaa
     2701 tgcctttgat atcatttatg aagcgggtgc cgattacctc gaaatcaaag acctgaagag
     2761 tttttcggaa tattggaaaa agcgtaaacc ttattgaatt ccgctactaa catttaggct
     2821 ttcataatta tgtgagtaaa cctgcgttgg ggtttgaaat ccctgcaatt ttacttcatg
     2881 ctttcgcgcg tttccaaaac cattcggatt atctggaaat acacccgcaa acctttatta
     2941 ggactcgcgg ctgtactggg aattgtgctc attagtgctt ggttattggc tgcttttttt
     3001 gaagatgaag tcaaagcata tttcctccaa aaaatcaata cagagctcaa tactcgcgtc
     3061 gaaattggtg aaatacattt aagtctactc gatcactttc ctcaagccag tgtttccctg
     3121 caaaatgtaa aagccgatca ttccaaacct tatcgtggcg ctggaaaatt gctcgaagcc
     3181 caaagcatcg attttagttt tagcgtgtgg gatttattca gcggttccta tagtattcat
     3241 cgtattgacc tttccaatgg agcgatgaat attttgcgaa attccgacgg catcatcaat
     3301 tatcaattgc tcaaaccaca agagaaatca agcagttcgg acgaaaaatt tagcttcgaa
     3361 cttaaatcgc ttcacgtcaa tcaaatgaac atcctgattc gcgatgagcc ttcctccttc
     3421 tctactcttt tcttggtcaa cagcggacaa ttcaaaggag catttacgga tgatgtattt
     3481 gatctcgaca ttgataccga tgtacagcta actcatctta aaagcgacga aacttcttgg
     3541 ttaagtgata gacctgtgag tattgatgtt ggtttgaaaa tcaacaagag cactgatctt
     3601 tacacattta ccggaggaga tattcagata tcagatttac aattaacggt tgatggcagt
     3661 attcaaaatc aaagcgatcc tgtttttgat ttgaaattgg gaggtaaaga tttggacatc
     3721 agttcctttt tatcggtttt acctgccgga tacgatgaac agattaagaa atatcgcagc
     3781 gatggtaagt tttactgttc tgctgaactc aaaggtgttt ggggtaagaa aacgtatcct
     3841 ttaataacgg caagttttgg aattagtgaa ggtgaaatca cccaaaaaga aaacgatatt
     3901 caacttgaag gagtaaatct aattgggaca ttcaccaatg gctctcaaca taacatgacc
     3961 agcagtgaat tatcgctcaa acaagtgaga atgaaacttc ggggcggtga ggtaaaaggt
     4021 gatttattga tccgaaattt cagtcaacct tatatccaag tcaaagctga tgcatctctt
     4081 gctctgagcg atattcagca attcttcccg ctagatccat tgcaattaaa atcaggaac
//
LOCUS       DAOOQW010000214         4100 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_25902_length_4100_cov_4.855871, whole genome shotgun sequence.
ACCESSION   DAOOQW010000214 DAOOQW010000000
VERSION     DAOOQW010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4100
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25902_length_4100_cov_4.855871"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..574
                     /locus_tag="PK281_10120"
     CDS             <1..574
                     /locus_tag="PK281_10120"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOY49739.1"
                     /translation="HIRFKGKHCSIASLAEMKERVVTVNGLSKSFAMTGWRLGYIGAP
                     KWIAQACDKFQGQITSGTCTITQRAAIAALEADPEVIQPMVEGFHKRRNLMLGLLKEI
                     NELKVNEPEGAFYFFPDISAFLGRKWKGQPVNTSIELTMYLINEFHLATVGGEAFGCG
                     NSIRLSYATSEETIIESVKRLKAGLESLEK"
     gene            571..1575
                     /locus_tag="PK281_10125"
     CDS             571..1575
                     /locus_tag="PK281_10125"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-heptose synthase"
                     /protein_id="HOY49740.1"
                     /translation="MNQNELQRVFEQFPHKRVLIIGDVMIDSYVWGKVDRISPEAPVP
                     VVAVKQKEDRLGGAANVALNIKALGATPVLASVIGNDRGGDCFLGLLESEGLTSEGIV
                     RFPRQTTVKTRIIGNHHQLLRVDEEDDSLLAAREQDLFLNHVLKLINDSPIDVIIFED
                     YDKGVISENLIKEVVLRANQKNILTAVDPKKRNFQFYKGVYLFKPNLKEIREGLASEV
                     NPKSLESLSNIDSELRKLLHHTWSLITLSEHGMFASNGTEHVQIASRPVTISDVSGAG
                     DTVISTAALCLACGADIQLSTFLANLAGGSVCEKPGVVPVDATTLFTDAIAELKKLPE
                     "
     gene            1615..2343
                     /gene="ubiE"
                     /locus_tag="PK281_10130"
     CDS             1615..2343
                     /gene="ubiE"
                     /locus_tag="PK281_10130"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HOY49741.1"
                     /translation="MTKVTPYEHSTDSKRKQVEKMFDNISDNYDFLNRSLSFGIDIWW
                     RKKMLGKLKGIEINRLLDIATGTGDVAIAASKQLNPKSIIGVDISDGMLAVGRVKIEK
                     KGLSQIIELQNADSENLPFESNSFDAVTVAFGVRNFEHLSIGLQEMNRVLRPGGKVVI
                     LEFSRPRIFPIRQLYDFYFRYFCPWWGKLISKDNSAYRYLYESVSVFPEGDEFLTIAK
                     NSGFKDLQAQRVTFGIVSLYTGIK"
     gene            2421..3104
                     /locus_tag="PK281_10135"
     CDS             2421..3104
                     /locus_tag="PK281_10135"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HOY49742.1"
                     /translation="MLGSSATTLSAQKRKVLNLPNYDKQTIHFGFLLGTNSFNFIPTP
                     VKDLRDIDSVLTIQQGAATGFTLGIISNLHMGDNFDLRFMPTLSFGERRLDYKIRLVS
                     QNDSIINRAKRVESTFLEFPLLLKFKSSRLNNGRAYIIGGVKSVIDLASQDKVDDKGE
                     KILKLKRNDFNYEIGFGFDFYSQYFKFTPEFKMSYGLKNLLVQDNNLYTLGLSSIHSK
                     AFYISFTFE"
     gene            3108..>4100
                     /locus_tag="PK281_10140"
     CDS             3108..>4100
                     /locus_tag="PK281_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006258371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HOY49743.1"
                     /translation="MHQELQLTLSPRLGYDSQLLKEHIRQELGLLDADRLHIIPLRRS
                     IDARGINVKVNLKVAVYVNEEQPVSNIQIPSYKNVVNAPEVIIAGAGPAGLFAALRLM
                     ELGLKPIVLERGKNVRDRRRDLAAINKEQIVNPDSNYCFGEGGAGTYSDGKLYTRSNK
                     RGDIRAVLDTFFAHGAVEEILIDTHPHIGTNKLPGIIVAMRETILNHGGEVHFNTRIS
                     EFLFEGAAFKGVKSHDGTKIPAQAIILATGHSARDIYEMLHAAGMHLEFKPFALGVRA
                     EHSQELIDSIQYHCDHRGDYLPPSSYSLVEQVKERGVYSFCMCPGGIIAPCATSP"
BASE COUNT         1314 a          696 c          845 g         1245 t
ORIGIN      
        1 gcacattcgt ttcaagggca aacattgcag tatcgcatcc ttagccgaaa tgaaagagcg
       61 agttgttacc gttaatggat tatcgaaaag ttttgcaatg acaggctgga ggctaggata
      121 tattggtgct ccaaaatgga ttgctcaagc ttgtgataaa tttcaaggac aaattacttc
      181 aggcacttgc actatcacac aacgagctgc aattgcagct ttagaagctg atccagaagt
      241 aatacaaccc atggttgaag gttttcataa aagaagaaac ctcatgcttg gtttgttaaa
      301 agaaatcaat gagttaaagg tgaatgaacc tgaaggtgct ttttacttct tccctgatat
      361 ctctgctttt ctaggtagaa aatggaaagg tcaacctgta aatacgagca ttgaactaac
      421 aatgtatcta attaatgagt ttcatttagc tactgttggt ggagaagcat ttggatgtgg
      481 aaattcgatt cgattatcat atgccacttc tgaagaaaca attattgaat ctgtaaagcg
      541 acttaaagca ggattggaaa gtttagaaaa atgaaccaaa acgagctaca gcgggtattt
      601 gaacagttcc ctcataaacg agtcttgatc attggagatg taatgataga cagttatgtg
      661 tgggggaaag tggatcgcat ttctccagaa gcacctgtac cagtagttgc agtaaaacaa
      721 aaagaagatc gattaggtgg tgcagcaaat gtggcattga acatcaaagc tttaggagca
      781 acacctgttt tagcttcagt aattgggaat gatcgaggtg gtgattgttt tttaggattg
      841 ttggaatcag aaggattgac tagcgaagga attgttcgat tcccaagaca aacaaccgta
      901 aaaactagaa ttattggtaa tcatcatcag ttattacgtg tagatgagga agatgatagt
      961 ttgttagctg ctagagaaca ggatttgttt ttaaatcatg tactgaaact gattaatgat
     1021 tcgcctattg atgtaataat atttgaggac tacgacaaag gagttatctc agaaaactta
     1081 attaaagagg ttgtactacg tgcaaaccaa aagaacatat tgactgcagt agatcccaaa
     1141 aagcgaaact ttcaatttta caaaggagta tatttgttta aacccaattt aaaagagatt
     1201 cgagaaggct tagcttcaga agtaaatccg aagtcgcttg aatcactttc caatattgat
     1261 tcagaattga gaaaattgtt gcaccatact tggagtttga taacactttc agaacacggt
     1321 atgtttgcta gtaatggaac ggaacatgtt caaatagcat cacgtccagt aactatttca
     1381 gatgtaagtg gtgctggtga tacagtaatt agtacagctg ctttgtgttt ggcatgcggt
     1441 gccgatattc aactgagtac atttctagca aatttagctg gaggaagtgt atgcgaaaag
     1501 cctggagtag ttcctgtcga cgcaaccacc ttatttacag atgctattgc agaactcaag
     1561 aaattacctg aataacaatc agaacaaaga caattgaagc tttgttcctt ctatatgact
     1621 aaagtaaccc catacgaaca ttctaccgat tccaaaagaa agcaggtaga aaaaatgttc
     1681 gataatatat ctgacaatta cgactttcta aatcgttctt tatcatttgg aattgatatt
     1741 tggtggagaa aaaagatgct tggaaagttg aaagggattg aaattaatcg acttcttgat
     1801 attgccacag gaacgggtga tgttgccatt gcggcatcca aacaacttaa tccgaaatca
     1861 attattggtg ttgatatttc agatggaatg ttagctgtag gaagagtgaa aattgaaaag
     1921 aaaggactca gtcaaataat agaattacaa aatgctgatt ctgaaaactt gccatttgaa
     1981 agtaattcgt ttgatgcagt aactgtagct tttggagtac gtaatttcga acatctttcg
     2041 attgggctcc aagagatgaa tcgagttctt cgcccaggag gcaaggttgt tattctcgaa
     2101 ttttctcgcc ctagaatttt tccaattaga cagttatatg atttctattt caggtatttt
     2161 tgcccatggt gggggaaatt gatttccaaa gacaattcag catatcgata tttatatgag
     2221 tcggtaagtg tttttcctga aggagatgaa tttttaacta ttgcgaaaaa ctcaggcttt
     2281 aaagaccttc aggcacaacg tgtaactttt ggaatcgtaa gtctgtacac gggtataaaa
     2341 taaaccttat tggttttgcg ttgaaacaga ttattaccaa aacagtgcat ttcactcgat
     2401 taatcctaac tctagtattg atgttaggat cttctgctac aacgctatca gctcagaaaa
     2461 gaaaagtgtt gaatttgcct aattatgaca agcagaccat tcacttcggt ttcttgttgg
     2521 gtacgaattc atttaacttt atacctactc ctgttaaaga tttgcgtgat attgattctg
     2581 ttcttactat tcaacaagga gctgcaacag gatttacatt agggataatt tctaatctcc
     2641 acatgggaga taattttgat ttaaggttta tgccaacgtt atcctttggt gaaagaaggc
     2701 tagattataa aataagatta gtatctcaga atgattctat aatcaataga gccaaacgag
     2761 ttgaatcaac tttccttgag tttcctttac ttttaaaatt taagtcatcc cgcttaaata
     2821 acggcagagc atatataatt ggtggagtga agtctgtaat tgacttagca tctcaagata
     2881 aagtagatga taaaggcgaa aagatactaa aacttaagcg taacgatttc aattatgaaa
     2941 ttggatttgg attcgacttc tattcgcaat atttcaaatt cactcccgaa tttaagatgt
     3001 cttatggact aaaaaaccta ttagtacaag acaacaacct ttatacatta ggcttaagta
     3061 gtatccattc aaaagctttt tatatctcat ttacatttga ataagtcatg catcaggaac
     3121 tgcagttaac actcagccct cgacttggct atgattcaca gcttctcaaa gaacatattc
     3181 ggcaagaatt aggattgttg gatgctgatc gattgcatat cattcccttg agacgctcga
     3241 ttgatgcacg tggcataaat gtaaaagtga atctgaaagt tgccgtgtat gttaatgaag
     3301 agcaacctgt ctctaatatt caaattccta gttataaaaa cgtagttaat gctccagaag
     3361 ttattattgc cggagcgggt ccagctggct tatttgcagc attgcgcctt atggaattag
     3421 ggctcaaacc cattgttctt gaacgcggaa aaaatgtccg tgatcgacgt cgcgatttag
     3481 cagccatcaa caaagaacag attgtaaatc ctgattccaa ttattgcttt ggtgaaggtg
     3541 gagcgggaac ttattcagac gggaaacttt acacacgttc taataaacgc ggtgatatta
     3601 gagccgtttt ggatactttt tttgcgcatg gtgcggtaga agaaatccta attgatacgc
     3661 atcctcatat tggtaccaac aaactacctg gaatcattgt cgctatgcgc gaaaccattc
     3721 tgaatcatgg tggcgaagtg catttcaata cgcgaatcag tgaattcctt tttgaaggag
     3781 ctgcttttaa aggtgttaag agtcatgatg gaactaagat tcctgcgcaa gcaattatcc
     3841 tagctaccgg acattcggca agagacatct acgaaatgct tcatgcagca ggaatgcatc
     3901 tggaatttaa gccgtttgca ttaggtgtgc gcgctgaaca ttcacaggaa ttaattgatt
     3961 ctatccaata ccactgcgat catcgaggtg attatttacc accttcctcc tactccttgg
     4021 ttgaacaagt gaaagaaaga ggtgtatact cattttgtat gtgtcccggt ggaattattg
     4081 cgccttgtgc aacatcgcca
//
LOCUS       DAOOQW010000215         4081 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_26071_length_4081_cov_6.481123, whole genome shotgun sequence.
ACCESSION   DAOOQW010000215 DAOOQW010000000
VERSION     DAOOQW010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4081
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26071_length_4081_cov_6.481123"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1245)
                     /locus_tag="PK281_10145"
     CDS             complement(<1..1245)
                     /locus_tag="PK281_10145"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HOY49744.1"
                     /translation="MERRTLFADVIVPLALPRLFTYRVPQILAPFIQPLQRVVVPFGK
                     KKKYTAIVHHLHEKPPQGYEARYIEQLLEEEPGISPQQLQFWEWMASYYLCHLGEVMQ
                     AALPAGLKLSSETVIRAVTTLEETDTTALSDRELLILEALEKVKELSIKDVEHIAGIK
                     TVYPIIRSMIQKRLIAAEDELKDLFRHRTITLVHLHTDFQGEDALHGLLNQLKRAPKQ
                     QELILAFLSMNPGKTHDFPAMKRKELLAKTGQTPATVMQLVKKGIFVLEDVPVSRLSG
                     QADDEAFIKLSPAQEEALSQIQESFAEGRPALLHGLTGSGKTEIYIQLIEEQIQKGKQ
                     VLYMLPEIALTAQIINRLRVHFGRKVQVYHSRFSENERVEVWNAVANCDPDAGMIVLG
                     ARSSVFLPFRNLGLILVDEEHES"
     gene            1351..1617
                     /locus_tag="PK281_10150"
     CDS             1351..1617
                     /locus_tag="PK281_10150"
                     /inference="COORDINATES: protein motif:HMM:NF024086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOY49745.1"
                     /translation="MQTIYKNCQSCGMPMKRDAQGGGTNADGSRSTVYCSHCYLNGSF
                     TMPHLKVSDMQVRVKGKMREMGMPGFVAGFFTRSIPKLERWKNN"
     gene            complement(1618..2382)
                     /locus_tag="PK281_10155"
     CDS             complement(1618..2382)
                     /locus_tag="PK281_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014085185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HOY49746.1"
                     /translation="MRIVTYNVNGLRSALSKGLVEWIQATQPDLLCLQEIKSQADQLD
                     LDLFRQLGYEPYLFPAQKKGYSGVAILSKHQPDRIIEGMGMEAYDFEGRTIRLDFGDW
                     TIINTYFPSGSSGEERQAFKMQFLADYQDFINELKKERPQLIICGDYNICHRAIDIHN
                     PVSNAKSSGFLPEERAWMESFLNSGFVDSFRHLNKDPHHYTWWSYRAGARGKNLGWRI
                     DYACITGNLQDKLQRAVILPEAKHSDHCPVLIEIDL"
     gene            2594..3964
                     /locus_tag="PK281_10160"
     CDS             2594..3964
                     /locus_tag="PK281_10160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49747.1"
                     /translation="MMDGIISAQTASSLRTRKVLLQGDTIRLDSMSIVQGSFRFVQSG
                     KDSAAYRLLADEGLLVRKPGIKNTSTDSALIQFRVFPFNFTESVGNKQRQRATAEQRG
                     IYNPFVYTPSKSNENPLKFDGLNKSGSISRGISVGNNQDLSVNSTLNLQLSGKLSSDI
                     DISAAITDDNIPIQPEGNTQQLQDFDRVYIQLSNEKSKLIVGDFQITRPESYFMNFNK
                     RLQGGSFETKFSTQILDDKKFAPAQVKIGASAAVARGRFNRNQIQGIEGNQGPYRLRG
                     ANNEQFIVILSGSEKVYIDGKQLKRGQEYDYVIDYNSAELTFTSRRLITKDLRIFVEF
                     EYSDRNYARSLLFFANEVEQGKTTTRIHVYSEQDSRNQPLQQQLNDEQKLILREAGDD
                     PQLAFIPAVDSVAFSTDLILYDMRDTVVNGTVYSNILVFSTNQIRHYTKLVSVMWART
                     RAIMCR"
BASE COUNT         1153 a          837 c          895 g         1196 t
ORIGIN      
        1 cgattcgtgt tcttcatcca ccaaaatgag tcctagattt cgaaatggca aaaacacgga
       61 agagcgcgcc ccaagaacaa tcattccagc atcgggatcg caattagcga ctgcattcca
      121 tacttccaca cgttcgttct cactaaaccg actgtgatac acctgaactt ttctgccgaa
      181 atgcaccctc aaacggttga tgatttgtgc tgttagcgca atttcgggaa gcatataaag
      241 tacctgcttt cctttctgaa tctgctcttc gatgagctga atgtagattt cggttttgcc
      301 acttcctgtc aatccatgca acaaggctgg tcgaccttcg gcaaatgatt cttgtatttg
      361 cgaaagtgct tcttcctgcg ccggacttaa ctttataaat gcttcatcat cggcctgtcc
      421 actcaaacga ctcacaggaa catcttccaa aacaaaaatc ccctttttca ctaactgcat
      481 taccgtggcg ggcgtttgtc ctgttttggc gagcagttct tttcgcttca tggcagggaa
      541 atcatgcgtt ttcccaggat tcatgctcaa aaatgcgaga atgagttctt gctgtttagg
      601 agctcgtttg agttgattga gtagtccgtg taatgcatct tccccttgaa aatcagtatg
      661 aagatgaacc aaagtgatgg tgcggtgacg gaacagatcc ttgagttcat cctcggctgc
      721 tatgagccgt ttttgaatca tgctccggat gattggataa accgttttta ttccggcaat
      781 gtgttctacg tctttgatgc ttaattcctt gactttctcg agtgcttcca aaatgagaag
      841 ttcacggtcg cttagcgcag tagtgtcggt ttcttcgagt gtagtaactg cccgaatgac
      901 cgtttcactg gagagtttga gtccggctgg taatgcggct tgcatgactt cacccaaatg
      961 gcagaggtaa tacgaagcca tccattccca aaactgtaat tgttgtggag aaatgcccgg
     1021 ttcttcttcg agcaattgtt cgatataacg cgcttcgtag ccttgaggag gtttctcatg
     1081 caggtgatgc acgatggccg tgtatttttt ctttttcccg aaggggacaa ctacccgttg
     1141 aagcggttgg atgaacggtg ctaagatttg aggaacgcga taggtgaaca gtcgtggcaa
     1201 agccagcggc acgatcacat cagcaaataa ggttctgcgt tccatggttg caaagatgca
     1261 gaaatgagtt caggctttga tttttttacc gtacagcttg caatcgtttc gatttttttc
     1321 tactttaaat cactcttaac cggataaacc atgcaaacga tttataaaaa ctgtcagtct
     1381 tgcggtatgc cgatgaagcg tgatgcgcaa ggaggaggaa caaacgccga tggttcaaga
     1441 agtactgttt attgttctca ttgttacctc aacggtagct ttacaatgcc tcatctaaaa
     1501 gtaagcgaca tgcaagttcg tgtaaaaggg aaaatgcgtg aaatgggcat gcctggcttt
     1561 gttgctggct ttttcactcg aagtattccc aagctggaac gttggaaaaa caattaatta
     1621 caaatcgatt tcaatcagta ccggacaatg atcgctgtgt tttgcttcgg gaagaataac
     1681 tgcgcgttgt aatttatctt gtagatttcc tgtgatgcaa gcataatcta ttcgccaacc
     1741 caagttttta ccacgagcac cagcacgata actccaccaa gtataatgat gtggatcttt
     1801 atttaaatga cggaacgaat caacaaatcc ggaatttaga aaactctcca tccaagctct
     1861 ttcctctggc aaaaatccgc tgcttttggc gttactaact gggttgtgga tgtcgattgc
     1921 tcggtgacaa atattataat caccacaaat aattagttgt gggcgctctt tcttcaattc
     1981 attgataaaa tcctgataat cggccaggaa ttgcatttta aacgcctgac gctcttcccc
     2041 actcgatccg cttggaaaat aggtgttgat tatggtccaa tcaccaaaat caaggcggat
     2101 agttcttcct tcaaaatcgt aagcttccat tcccatacct tcaattatcc ggtcgggttg
     2161 atgcttactc aaaatagcaa caccgctata tcctttcttt tgagcaggaa ataaataagg
     2221 ctcgtatcct aactgacgga ataaatctaa atcaagctga tcggcctgac tttttatctc
     2281 ctgcaaacaa agtaaatcgg gctgagtcgc ctgaatccac tctactaatc ctttactgag
     2341 agcggaacga agtccgttta cattataggt aacaatgcgc attgaaaatg tttttgaaag
     2401 gagcaaagaa aggaaaatta atgtgctgat gtgctagtgt gttgatgtgc tggtggtggc
     2461 gatgaggcaa ttccaaacat tttatggtga atgatatata aagtacgcat tctgacgttg
     2521 atgtgcattt ggactatctt cgccggaagg gcaaattgtg cagcgtttac tactgattct
     2581 cttggttttt ctgatgatgg acgggattat ttccgcccaa acagctagta gtttgcgtac
     2641 acgaaaggtc ttgttacaag gcgatacgat tcgcttggat tcgatgagta ttgtgcaggg
     2701 gagttttcga tttgttcagt ctggaaaaga cagtgctgca tatcgtttac tggcggatga
     2761 aggattgctg gttcgaaaac ccgggataaa aaacacctca acagattctg ctctcataca
     2821 atttcgtgtg tttcctttca attttaccga atcggttgga aataagcagc gacagcgtgc
     2881 tacagccgag caacgtggta tttataatcc attcgtttat acgccttcga aaagcaacga
     2941 aaatccgctc aaattcgatg gattaaataa atcgggctct atctctagag gaatttcagt
     3001 gggaaataat caggatttat cagtcaactc gactttaaat cttcaattat ccgggaaatt
     3061 aagttccgat atcgacataa gtgctgctat tactgatgat aatattccta ttcaacccga
     3121 aggaaacacc cagcaattgc aggacttcga ccgcgtttac attcaattat ccaatgaaaa
     3181 atcgaagtta attgttgggg attttcaaat tactcgcccc gaaagctatt tcatgaattt
     3241 caataagcga ttgcaaggtg ggagtttcga aacgaagttc agtacacaga ttttagatga
     3301 taagaaattc gcgccagcac aggtgaaaat tggtgctagt gctgctgttg cacgaggacg
     3361 atttaatcga aatcaaattc aaggaattga gggaaaccaa ggtccatatc gactacgcgg
     3421 ggcgaataat gagcagttta ttgtcattct ttcgggttcg gaaaaagtgt atatcgatgg
     3481 aaaacagctc aaacgcggac aggaatatga ttatgtgatt gattacaact cagcagaatt
     3541 aacgtttacc tcgcgccgat tgatcacgaa agacttgcgg atttttgtcg agtttgaata
     3601 ttcggatcgg aattatgcgc gttcactttt gttttttgca aacgaagttg aacagggaaa
     3661 aacaactaca cgaattcatg tgtattctga gcaggatagc cgaaatcagc ctttgcagca
     3721 gcagttaaac gatgaacaaa agctcattct gagagaagct ggtgatgacc cacaattagc
     3781 ttttattcca gctgttgata gtgttgcatt ttcgactgat ttaattttat acgatatgcg
     3841 cgacactgtc gtaaatggaa ctgtttattc aaacatttta gtattctcga ccaatcagat
     3901 tcggcactat acaaagctag tttcagtaat gtgggcgcga acaagggcga ttatgtgcag
     3961 ataaatagta tcgcaaatgg acgagtttac aaatgggttg ttcctgtaaa tggaattcct
     4021 cagggaaatt acgtaccaaa aacacaactc attccaccaa taaaacgaca agtggtgact
     4081 g
//
LOCUS       DAOOQW010000216         4036 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_26490_length_4036_cov_4.095453, whole genome shotgun sequence.
ACCESSION   DAOOQW010000216 DAOOQW010000000
VERSION     DAOOQW010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4036
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26490_length_4036_cov_4.095453"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1026)
                     /locus_tag="PK281_10165"
     CDS             complement(<1..1026)
                     /locus_tag="PK281_10165"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOY49748.1"
                     /translation="MPSEFETLLVGGEKDDSEDSSEFIVKNLGLDPIIIPEMKREIDL
                     KNDYEAYRKMVKLIQDFKPDIVHTHASKAGTIGRLAAANMNVPVIIHTFHGHVFHSYF
                     GKMKTVMYKNIERNLARRSSTIIAISEKQKTELCLIHRICKPEKIRVVPLGFDLSRFT
                     ENMEEKRKSFRSRYHLADDEIAVGIIGRLVPIKNHKLFIEACKLVLDQTKNKVRFFII
                     GDGEDRLTIEAMAREAGIPFVDAMLSNDKAPLTFTSWIKDIDVACAGLDIIALTSLNE
                     GTPVSLIEAQAANKPIVTTNVGGIENVVIEGVTALLSHSKTDPKQFADLLLRLTENAD
                     LRTEMSRV"
     gene            complement(1137..3026)
                     /gene="asnB"
                     /locus_tag="PK281_10170"
     CDS             complement(1137..3026)
                     /gene="asnB"
                     /locus_tag="PK281_10170"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HOY49749.1"
                     /translation="MCGISGYYSFTPKGKEQLQHIDASVAALALRGPDGSGIFKHNSV
                     ALGHARLSIIDVSNAASQPFTDETGRWTMVFNGEFFNFQEHREALEKAGQTFRSTSDT
                     EVLLYLYIREKEACLHKINGFFAFAVYDNQTEELFIARDRYGERPLLIAQTEDGIAFA
                     SEMKSLLTFDWPRNLDNEAARQYFHLNYIPGPESIFNSVKKLQPGTWMRLNGSKIEQK
                     SWYNFPPAPTKQDISIDYTSSQKELIKLLDAAVERRLISDVPLGAFLSGGIDSSVVVA
                     LASRHTNHLNTFSIGYKDEPLFDETKYARLVVDRYKTNHTVFSLSNEDLFKHLHDVLD
                     YIDEPFADSSALAVYILSKETRKHVTVALSGDGADELFGGYRKHSAEWKMRQGGLAAS
                     TVKLLGPVWNALPKSRNSKLGDIARKLQKFSEGANLSTPERYWRWCGYSDEAYLNELW
                     AGPFNQQKGQEYKRNHLSSIGTTGDMNDMLRSDMLLVLPGDMLTKVDMMSMANSLEVR
                     APFLDVEVVDFAMRLPADYKISGAGRKRLVQDAFRSMLPEELYNRPKHGFEVPLLSWF
                     RNELNSCIFGELLSPQVLKEQGLFHLSAIEKLKKQLHSSDPGDSAAKIWALIVFQSWW
                     KKRMK"
     gene            complement(3066..>4036)
                     /locus_tag="PK281_10175"
     CDS             complement(3066..>4036)
                     /locus_tag="PK281_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOY49750.1"
                     /translation="TGARSRELPNLKQDGKKIIGYRQAMTLPKQPKSMVVVGSGAIGI
                     EFAYFYNAIGTKVTVVEFLPNIVPVEDEEVSKQLERSLKKSGISIMTDASVESVDTSS
                     DVLKVKVKTKKGEEILEADVVLSAVGIVSNIENIGLEEAGVKTDKGKVLVDDFYKTNI
                     DGIYAIGDIVKGPALAHVASAEGIICVEKIAGHHPAPLNYGNIPGCTYCSPEIASVGM
                     TEKAAREAGYEIKVGKFPFSASGKASAAGAKDGFVKVIFDAKYGEWLGCHMIGANVTE
                     MIAEAVVARNLETTGHEIIKSVHPHPTMSEAIMEAAAAAYGEVIHL"
BASE COUNT         1169 a          900 c          716 g         1251 t
ORIGIN      
        1 tacacgcgac atttctgtcc gtaaatcagc attttcggtt aagcgtaata ataaatctgc
       61 aaattgtttt ggatctgttt tagaatgtga taataaggca gttacacctt caataaccac
      121 attttctatt cctcccacat tggtagttac aataggctta ttggctgctt gagcttcaat
      181 taaacttact ggagtacctt catttaaaga tgttaatgct atgatatcca agccagcaca
      241 ggcaacatca atatctttaa tccaggatgt gaaagtgagt ggagctttat cattacttaa
      301 cattgcatca acaaatggaa tccctgcttc acgagccatt gcttcaatgg ttaatcgatc
      361 ttctccatca ccaataatga aaaaacgaac cttgtttttg gtttgatcta acaccaattt
      421 acaagcttct ataaatagtt tatgattttt aattggaacg agtcgtccaa taatgccaac
      481 cgctatttca tcatcagcca agtgatatcg actgcggaaa gacttgcgtt tctcttccat
      541 attctcggta aaacgcgaca aatcaaaccc caatggaact actcgaatct tttctggttt
      601 gcaaatacgg tgaattaaac agagttctgt cttctgtttt tcactaatag caatgattgt
      661 agaacttcga cgtgctagat ttctttcaat gtttttatac atcaccgttt tcatttttcc
      721 gaagtaggaa tggaaaacgt gtccatgaaa cgtatgtata atcacaggta cattcatatt
      781 agctgcagct aatcgaccta ttgtaccagc cttggatgcg tgtgtgtgaa caatatcggg
      841 tttgaaatct tgtatcaact tgaccatttt gcgatacgct tcatagtcgt ttttcaagtc
      901 aatctcccgc ttcatttcgg gaatgataat cggatctaac cccagatttt ttacaatgaa
      961 ttcggaacta tcttccgagt cgtccttctc tccacctacc aacaaagttt caaattccga
     1021 aggcatatac cgcgataaat acgcagcatt ataggttggt cccccgagat tgaatcgatt
     1081 tattatcctt aatattttgg gcataagtgg ttgagcttag gcgggctcaa atgtatctat
     1141 ttcattcgct ttttccacca agattgaaat acgattaaag cccaaatttt tgcagccgaa
     1201 tctcccggat cagatgagtg tagttgtttt tttaattttt caatagcaga taaatggaaa
     1261 agtccctgtt ccttaagcac ttgtggggaa agtaactcac caaaaataca ggaattcaac
     1321 tcatttctaa accaagataa taagggtact tcaaatccat gtttcggtcg attgtacaat
     1381 tcttctggta acatcgaccg aaaagcatcc tgaactaatc gcttacgacc tgctcctgaa
     1441 atcttataat ctgcaggtaa gcgcatcgca aaatcaacta cttccacatc taaaaatggt
     1501 gctcttacct ctaaactatt cgccatcgac atcatgtcca ccttagtcaa catatcaccc
     1561 ggaagtacaa gtaacatatc gcttctaagc atatcattca tgtcgcctgt tgttcctatt
     1621 gaactcaagt gatttctttt gtattcctgt cctttttgtt gattgaaagg tcctgcccat
     1681 aattcattca aataggcttc gtcggaataa ccacaccaac gccaatagcg ttcgggagtg
     1741 ctcaaatttg ctccttcgct gaatttctga agtttacgag ctatatcacc caatttacta
     1801 ttgcgtgatt tcggaagcgc attccaaact ggacctaaaa gttttaccgt actagcagct
     1861 agtccacctt gacgcatttt ccattcggcc gagtgttttc ggtaaccacc aaataattca
     1921 tccgctccat cacccgataa agccactgtt acatgtttac gagtttcctt acttaaaata
     1981 tatacagcga gagctgatga atcggcaaat ggttcatcga tataatctaa aacatcatgt
     2041 agatgcttaa acaaatcttc gttgcttaag gaaaacacgg tgtgattggt tttataccga
     2101 tccaccacca aacgagcata ttttgtttcg tcgaaaagtg gttcgtcttt ataaccaatc
     2161 gaaaaggtat tcaaatgatt cgtgtgacgg gatgccaagg ctacaactac cgaagaatct
     2221 attccaccac tgagaaatgc acctaatgga acatccgaaa tcaagcgacg ctcaacagct
     2281 gcatctaaaa gttttattag ctctttttgt gatgaagtat aatcaatgga aatgtcctgt
     2341 tttgtgggag caggagggaa gttgtaccag ctcttttgct cgattttact tccattcaag
     2401 cgcatccaag tccccggttg taatttcttt accgaattga aaatgctctc tggaccaggt
     2461 atgtaattga gatgaaaata ctgacgagca gcttcattgt ctaagtttct aggccaatcg
     2521 aaagtcagca aagatttcat ttctgaagca aaagcaatcc catcttcggt ttgagcgatt
     2581 aataaaggcc tttcgccata acgatctcgt gcaataaata gctcttctgt ttgattatca
     2641 tagaccgcaa atgcgaagaa cccgttgatt ttatgtaaac aggcttcttt ctcccgaatg
     2701 tagagataca acaaaacctc tgtatcactt gtggatcgaa aggtttgtcc tgctttttct
     2761 agtgcttcgc gatgttcttg gaaattgaaa aattccccat taaacaccat tgtccatcta
     2821 cctgtttcat cggtaaacgg ctgagaagca gcgttactaa catcgataat ggataatcga
     2881 gcatgtccga gtgctacaga attatgctta aaaatccctg atccatccgg tccacgcaaa
     2941 gcgagagcag caactgaagc atcgatatgc tgcaattgtt ctttcccttt tggggtgaat
     3001 gaatagtatc cgctaattcc gcacatatag aaaaaagccc cgatcgaaat cgaggctttt
     3061 agaaattaaa ggtgaatgac ttctccgtaa gcagcagcag cagcttccat gatagcttcc
     3121 gacatcgttg gatgcgggtg aacactttta ataatttcgt gtccggtagt ttctaagtta
     3181 cgagcaacaa cagcttcggc aatcatttcg gttacgttag caccaatcat gtggcaacct
     3241 aaccattcgc catatttagc atcgaatatc actttcacaa atccatcttt tgcaccagca
     3301 gcagatgctt tacctgatgc agagaatggg aatttcccca ctttgatttc ataacctgct
     3361 tcacgagcag ctttttcggt cataccaact gaagcgattt cggggctgca atacgtacat
     3421 cctggaatgt tgccgtaatt aagcggagct ggatgatgtc ctgcaatttt ttctacacag
     3481 ataattcctt ctgcagaagc aacgtgagcc aaagctggac ctttcacaat atcaccaatg
     3541 gcataaattc catcgatgtt ggttttatag aaatcatcta ctagcacttt gcctttgtcg
     3601 gttttaacac cagcttcttc taatccaata ttctcgatat tcgaaacaat tccaacggca
     3661 gataaaacta catccgcctc aagaatttct tctccttttt tggtttttac tttcactttc
     3721 aacacatccg aacttgtatc taccgattca acagatgcat cagtcataat ggaaatgcct
     3781 gactttttca agctacgctc caattgcttg ctcacctctt catcttcaac cggaacaata
     3841 ttcggtaaga attccaccac tgttactttc gttccaatag cattgtagaa atatgcgaac
     3901 tcaattccga tagctccaga accaactaca accatcgatt taggttgttt aggtaaagtc
     3961 attgcttgac gataaccaat gatttttttg ccgtcttgtt tcagattagg taattcgcga
     4021 ctgcgagcac ctgtag
//
LOCUS       DAOOQW010000217         4025 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_26608_length_4025_cov_4.875063, whole genome shotgun sequence.
ACCESSION   DAOOQW010000217 DAOOQW010000000
VERSION     DAOOQW010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4025
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26608_length_4025_cov_4.875063"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            97..627
                     /locus_tag="PK281_10180"
     CDS             97..627
                     /locus_tag="PK281_10180"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HOY49751.1"
                     /translation="MDHLNALDYLILAFLLWGFIAGFRRGLIMELCTLAGLFLGIWLA
                     IHFSGAAEEWMKTSQKMEGGWISFVAFLMVFVGVYIGFYFLGKALSKAVSLLMLGIIN
                     RIAGGIFGMIKMMMFSSILVLILKMGGIPLLSEELEKGSAIFKPVSAFAELVYPSISD
                     ILPEKKESPVEELLES"
     gene            681..1769
                     /locus_tag="PK281_10185"
     CDS             681..1769
                     /locus_tag="PK281_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018944891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HOY49752.1"
                     /translation="MITKEQVLDALSNVMEPDLKKDLVTLNMIQDVAVDGIKVSFTVV
                     LTTPACPLKELIHNACVNAIKHLIDMDAEVTVNMTANVTTKRGEERPLLPKVKNIIAV
                     ASGKGGVGKSTVAANLAVSLADLGARVGLVDADIYGPSIPIMFDVVDEKPMVRMVDGQ
                     QMMIPVESYGIKLLSIGFFADTSQAIVWRGPMASKALTQLFRDADWGELDYLIIDLPP
                     GTGDIHLTLVSGIPLTGAVIVSTPQRVALADAEKGISMFQLPNINVPVLGVVENMSYF
                     TPEELPDNKYYIFGKDGAKDLAEKLGVPLLGQIPLVQSIREAGDSGRPAGLQENTPVA
                     NAFLELAKNVAQQVAIRNAEVAREAVSH"
     gene            1801..2067
                     /locus_tag="PK281_10190"
     CDS             1801..2067
                     /locus_tag="PK281_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008236900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HOY49753.1"
                     /translation="MQPEEKLALKLKVEQAIDSIRPYMEADGGNITVDEITDDLVAMV
                     RLHGACSSCSMSTMTLRSGVTEAIRHAVPEIRDVVAVNMPEVAF"
     gene            2081..3307
                     /locus_tag="PK281_10195"
     CDS             2081..3307
                     /locus_tag="PK281_10195"
                     /inference="COORDINATES: protein motif:HMM:NF012363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY49754.1"
                     /translation="MNISVHISRVTYLLGLSLLSLNLSAQYDCSNDRFLQEVFTDVSV
                     TSDVEYGNAFTYSGAATILKMDVYQPTGDAFTERPLIVFAHGGSFIFGNKTSSDIVTL
                     CNKYTRLGYVCASIDYRLGYENLIPNATTATETVYRATSDMRAAIRYFRKDRATANQF
                     KIDTTNIYAAGVSAGAFMVLHLAYLDQPEEIPTAVDPVAFGGIEGLSGNDGYSSKVKA
                     IVNLCGALGDTAWINAGDTPCLSMHGTNDDVVPYGSDIINVLGISLFEVDGSHSVAAR
                     QSHIGVQNEFYTWPGQAHVPFVNSTVYMDSVYMAMTPFLANQLGCEVPTAIAPTDAPV
                     ELAMWPNPASETIYLNSSAKINQVQIFNLQGKLVQSMNPSNSTTSVSIDNLPNGMYFI
                     RVLSNSTWQTRKFIKN"
     gene            complement(3363..>4025)
                     /locus_tag="PK281_10200"
     CDS             complement(3363..>4025)
                     /locus_tag="PK281_10200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49755.1"
                     /translation="NSVTYVYHPTGQLKEEQTFRGDKLIRKEVYDNEYNAAGKETQQC
                     RYQIDSAGARVPHNAPMTISEYDNEGLILQRTVYNNKEKRKMMSWIYYKYQLDNDYKV
                     IKQTGFNEEQQEIYRNELSYTDSSIISIEYKMCSCPEKKVEKAGSRQLIFNKFGEKTK
                     ELVYAANGDLTETRTWLYDDFGNMVENQTVKVAEPAKLIKSKMILEFQVDQAQTARKP
                     TK"
BASE COUNT         1093 a          802 c          851 g         1279 t
ORIGIN      
        1 gcaaaggtat tgctctgttg gcttatcaag gttcggtcgt tcaaaattta agtggattga
       61 tgtagaatcg gcaacagagc cgcatttctt tgcagcatgg accacctaaa cgcgctcgac
      121 tacctgattc ttgccttcct actttgggga tttattgctg ggtttcgtcg aggattgatc
      181 atggagcttt gcaccttagc tggactattt ctaggaatct ggctggcaat ccatttttca
      241 ggagcggcag aagaatggat gaaaacttcg cagaaaatgg aaggtggctg gatttcattt
      301 gttgctttct tgatggtttt tgtgggcgta tatattggat tttatttcct tgggaaagcc
      361 ttgtccaaag cagtaagctt gctcatgctt ggtataataa atcgaattgc tggaggaata
      421 tttgggatga ttaaaatgat gatgttttcg agcattctgg tgttaatcct taaaatggga
      481 ggcattccat tgctttcgga agaacttgaa aaaggctctg ctatcttcaa acctgtaagc
      541 gcttttgccg aattagttta tccatccata agcgacattc ttcccgaaaa gaaagaatct
      601 cccgtagagg aattattgga atcctgaaca agcacgattg ctggctgttt atagctaaat
      661 ttgcccctca attttcccac atgattacca aagaacaagt actcgacgcg cttagcaatg
      721 ttatggaacc ggatctgaaa aaagatttgg taacgcttaa catgattcaa gatgtagcgg
      781 ttgatggaat aaaagtgtcg ttcaccgtgg ttcttaccac tccggcttgt ccgctaaaag
      841 aactcatcca taacgcctgc gtaaatgcca tcaaacattt gatcgacatg gacgctgagg
      901 ttacggtaaa catgaccgca aacgttacta ccaagcgtgg tgaagaacgc cctttgcttc
      961 ctaaagttaa aaatataatt gccgttgctt ctggaaaagg cggtgttgga aaatcaactg
     1021 ttgctgctaa tctggcggtt tcacttgccg atttaggtgc tcgagttggt ttggttgatg
     1081 ccgatattta tggtccatct attccaatca tgttcgatgt agtggatgaa aaacccatgg
     1141 ttcgcatggt tgacggacaa caaatgatga ttccggtaga aagttacggt atcaaattat
     1201 tgtcgatagg tttctttgct gacacttctc aggcaattgt ttggcgtggg ccgatggcat
     1261 ccaaagcgct aacccaactt tttcgtgatg cagattgggg cgagcttgat tacttaatta
     1321 ttgatcttcc tccaggaaca ggagatattc acttaacgct tgtcagtggc attcctctaa
     1381 caggagctgt tattgttagc acacctcaac gtgttgcttt ggcggatgca gaaaagggaa
     1441 tttcgatgtt ccaacttccg aatatcaatg ttcctgtttt gggtgtggtc gaaaacatgt
     1501 cctatttcac tcccgaagaa cttcctgaca ataaatatta cattttcgga aaagatggag
     1561 caaaggattt ggcggaaaaa ctcggtgttc cattgttagg acaaattccg ctcgtacaaa
     1621 gcattcgtga agcgggcgat agtggccgtc cagcaggatt acaggaaaac acgccggtag
     1681 caaatgcatt cctcgaatta gctaaaaatg tagctcaaca agttgccatt cgaaatgcag
     1741 aagttgctcg agaggctgta tcgcattaat tgtatatttg aaaataatcg gaacaccatc
     1801 atgcaaccgg aagaaaagtt agctcttaaa ttaaaagttg aacaggcaat cgattccatt
     1861 cgtccttata tggaggccga tggaggcaat atcacggtag atgaaattac cgatgatcta
     1921 gttgcaatgg ttcgccttca tggagcttgc agttcgtgtt caatgagcac tatgacactt
     1981 cgttctggtg ttaccgaagc aattcgacac gccgttccag aaattcggga tgttgttgcg
     2041 gttaatatgc ccgaagtcgc tttctaaatc ccttctgctt atgaatatat ctgtacacat
     2101 ttcccgagtt acttatttgc tcgggctgag cttgctatca ttaaatctca gcgcccaata
     2161 cgattgttcc aacgaccgct ttcttcagga agtatttaca gatgtttctg ttacatccga
     2221 tgtggaatat ggtaatgcgt ttacttatag tggagcagca acgatattaa aaatggatgt
     2281 ttatcagcca acgggtgatg catttaccga gcgcccgctg attgttttcg cacatggtgg
     2341 ttctttcatc tttggaaata aaaccagttc cgacattgta actttatgta acaagtatac
     2401 ccgattaggt tacgtatgtg cctctattga ctatcgtttg ggttatgaga atttgattcc
     2461 taacgcaaca actgcaaccg aaacagtata tcgtgcaacc tctgacatgc gtgcagcaat
     2521 tcgctatttc agaaaagacc gagctacagc taatcaattt aaaattgaca cgactaatat
     2581 ttatgcagca ggtgtttcag caggagcctt tatggtgctt cacttggcct acctcgatca
     2641 accggaagaa attcctacag ctgttgatcc agttgctttt ggtggaattg agggtttaag
     2701 tggaaatgat ggctactctt ctaaagttaa agcaatcgta aatctttgtg gtgctttagg
     2761 cgatactgct tggattaatg ccggcgatac accttgtctt tctatgcatg gtacaaacga
     2821 cgatgtggtt ccttatggct cagatattat caacgtactt gggatttcat tgtttgaagt
     2881 agatggaagc cattcggttg ctgctcgtca aagtcatatt ggtgttcaaa atgaatttta
     2941 cacttggccc ggacaagcgc atgtgccttt tgtgaactct actgtttata tggattcggt
     3001 atatatggcg atgacaccct ttttggctaa tcagctaggt tgtgaagttc caactgcaat
     3061 tgccccaaca gatgcaccag tagaacttgc tatgtggcca aatccagcta gcgaaacaat
     3121 ttatttgaac agttctgcta aaatcaatca agttcagatt ttcaatttgc aaggaaaatt
     3181 agttcaatcc atgaatcctt ctaattcgac aacaagtgtt tcaatcgata atctgcctaa
     3241 tggaatgtac tttattcgtg tactctccaa ttcaacttgg caaacacgaa agtttataaa
     3301 gaactaaaaa caaaaaaccc cgaacgactt atcattcggg gtttttttat tgtctttttc
     3361 gattatttcg ttggcttacg agctgtttgt gcttgatcaa cttggaactc taaaatcatt
     3421 ttcgacttga tcaattttgc tggttctgca actttaacag tttggttctc gaccatattt
     3481 ccgaaatcat catacaacca agtgcgagtt tcagttaaat caccatttgc agcataaact
     3541 agttctttgg ttttttctcc aaacttgtta aaaatcagct gacggcttcc tgctttctct
     3601 actttctttt ccggacaaga gcacattttg tattcaatac tgataataga actatcggta
     3661 taggataatt cattccgata aatctcttgt tgctcttcgt tgaaaccagt ttgcttgatt
     3721 actttataat cgttgtcgag ctgatatttg taataaatcc aactcatcat tttacgtttc
     3781 tctttgttgt tataaacggt acgctgaaga atcaaacctt cgttatcgta ttcgcttatc
     3841 gtcatgggtg cgttatgagg aacacgagcg ccagcactat caatttgata ccggcattgt
     3901 tgtgtttcct ttccggcagc attgtattca ttatcgtaaa cttcttttcg aattaattta
     3961 tcacctcgaa aagtttgttc ctcttttagt tgacctgtcg gatgatacac ataggtcaca
     4021 ctatt
//
LOCUS       DAOOQW010000218         4017 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_26688_length_4017_cov_3.527764, whole genome shotgun sequence.
ACCESSION   DAOOQW010000218 DAOOQW010000000
VERSION     DAOOQW010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4017
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26688_length_4017_cov_3.527764"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="PK281_10205"
     CDS             complement(<1..275)
                     /locus_tag="PK281_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49756.1"
                     /translation="MLTALRLFLAFYCLAATEMVSAQQVVPILNYSTNANGQVELEVA
                     STPLFYYVLEVRHSPEGAFEIATSVTMGQPGSTVITEPLGNYPLENY"
     gene            complement(799..1938)
                     /locus_tag="PK281_10210"
     CDS             complement(799..1938)
                     /locus_tag="PK281_10210"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HOY49757.1"
                     /translation="MKYLVHLCRTLVGVLFIISGLIKSNDTIGFAYKLEEYYQVFGTE
                     FLISTAVLQAMVICIVEVVLGVMLLIGYRKKLTLFLLMAMIVFFTFLTFYSAYFNKVT
                     DCGCFGDALHLTPWQSFGKDVALLILISVLVAGSKHIQQLFSNKVTLGILSIALILNI
                     AFPVYTYNYMPVKDFRPYAIGNDIIEQMNGGRPGKFDTRFVYKNLQTGKDEEMVKPPY
                     TDTINWEIDTLKWKFVDTKQIVLDPGEQAPIHDFSITSVEGSEYTDDFLKHDSLHFFL
                     VSYDLTKADRDVQPQINAFAQACEKNGIPFIGLSSSTPEAIDEFRHEVQAPYDYYITD
                     ATQLKTMIRSNPGLILLKGSKILDMWGKRTLPTFEDFRKKYPNNK"
     gene            complement(1985..2530)
                     /locus_tag="PK281_10215"
     CDS             complement(1985..2530)
                     /locus_tag="PK281_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019388783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1599 domain-containing protein"
                     /protein_id="HOY49758.1"
                     /translation="MQQTSAQFDAVIQRCKDIFLKKNTDYGTSWRIFRISSITDQLFI
                     KASRIRGIETKGMHLVEEGIEPEFIGIVNYSLMGLIQMQLGTDSELELSRERSEQLYD
                     QTVAEVKALMMQKNHDYGEAWRDMRVSSFTDMILVRLMRIKQMEDNQGATLISEGIDA
                     NYMDMINYAIFALIKISEDEA"
     gene            2628..3404
                     /gene="folP"
                     /locus_tag="PK281_10220"
     CDS             2628..3404
                     /gene="folP"
                     /locus_tag="PK281_10220"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010135267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOY49759.1"
                     /translation="MGIININEDSFFAGSRVSDSNFLQLASRHVQEGAQFLDLGASSS
                     RPGALISQASDEWKRLQPVITAVRKEFPKSIISVDTYHAQVARWAVESGADLINDISA
                     GKIDNAMFSTVAELKVPYILMHMQGRPDSMQQNPAYKDVVYEVFHELHQSLEALRQVG
                     VEDVIVDPGFGFGKSLEHNYQLLNKLDVFHKLKIPLLVGVSRKSMLTRLLDVKVDDAL
                     NGSTVLHTIAIQKGAQILRVHDVKPAVEAIRILEMIRSVK"
     gene            3447..>4017
                     /locus_tag="PK281_10225"
     CDS             3447..>4017
                     /locus_tag="PK281_10225"
                     /inference="COORDINATES: protein motif:HMM:NF039908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase"
                     /protein_id="HOY49760.1"
                     /translation="MQLLISFFITLRWLDIVDIVIVAILLYQLYSLVRGTSAVSIFFG
                     ILLFYLLWLLVKALNMQLLATILGQFIGVGVIALIIVFQQELRRFLLMVGSRGIFNKE
                     NFKGFSGLNSAAYGSLNYESIVTACQKMALQHTGALIVISRKSDLKYYANTGERLDSE
                     ITANILESIFFKNNPLHDGAVIISNNRVKA"
BASE COUNT         1217 a          729 c          831 g         1240 t
ORIGIN      
        1 tggtaatttt ctagcggata atttccaagc ggttcggtaa tcacagtgct gccaggttgc
       61 cccatagtga ctgaagttgc tatttcaaac gcgccttcag gagagtgtct cacttccaat
      121 acataataaa acaatggtgt ggaagcaacc tccagttcga cttgtccatt ggcattagtt
      181 gaataattta gtattggtac cacttgttgt gcactaacca tttccgttgc ggcgaggcaa
      241 tagaacgcaa gaaataatct taaagcagtt agcatgcgca aagcggttta aaaaaaaatg
      301 cgagaatttg acagaaatga acgcctcgag gtgtcaaaaa aatcaattca ctaaaattaa
      361 tcaataaagc tgaaaaaagc tggatttatt cgaaatcggc aatcatcatt tcattaatgc
      421 ggctgaaatg cttactaaca agtagttttg ggtggatagt gttggacaat cttccaatac
      481 ccaatttcag ttcgttgcag agggttaatt tatttacgcc tttgcatttc tgatatggac
      541 ttaaatattg aaatcgttct gttagcttat tttattcttt aataatttcc gaatttttga
      601 gcgcagtttt agtaaaatcc aatcagttaa acgctaagag aattgctact cttatcaatc
      661 aacataggat ttttacagtt actattcgat tctcaacaag ctgtccgctg gggcaaatta
      721 aaattgcatc aacaagcaaa gcttcttgag gtaaaataat ttgaagcaga atccagatgc
      781 aaacttgaaa ttttctaatt acttattgtt agggtacttt tttctaaagt cctcaaaggt
      841 cggcaaggtt ctttttcccc acatatctaa aattttggaa cctttaagta gtattaatcc
      901 gggattggag cggatcatag ttttaagttg tgttgcgtcg gtgatgtagt aatcgtaagg
      961 tgcttgtact tcgtgacgga attcgtctat tgcttcaggt gtgctagaag ataagccgat
     1021 gaatggaatc ccgtttttct cacatgcttg cgcgaaggca ttaatttgtg gttgtacgtc
     1081 tcgatctgct tttgtcaaat cgtaactaac caagaagaaa tgcagactat cgtgttttaa
     1141 gaaatcatcg gtgtattcag aaccttctac gctagtaata ctgaaatcgt gaataggagc
     1201 ttgctcaccc ggatcaagaa cgatttgttt ggtatcaaca aacttccatt ttaaggtatc
     1261 gatttcccag ttaatcgtat cggtataagg cggtttaacc atttcttcat ccttaccagt
     1321 ttgaagattt ttatacacga aacgtgtatc gaatttcccc ggacgaccac cgttcatttg
     1381 ttcgatgatg tcgttgccaa ttgcatacgg acggaaatcc ttaactggca tgtagttata
     1441 cgtataaaca gggaaagcaa tattgagaat caaggcaatg gaaagaatgc cgagtgttac
     1501 tttattacta aataattgtt gaatgtgttt gcttccagcg actaatactg aaatcagaat
     1561 aaggagagct acatcttttc cgaaagattg ccaaggagta aggtgaagtg catcaccaaa
     1621 acatccacaa tcggttactt tattgaagta agcagagtag aaggtaagga atgtgaaaaa
     1681 cacaatcatg gccattaaga ggaacagagt gagtttttta cggtatccta ttaatagcat
     1741 cacgcctaaa acaacctcta caatacaaat gaccatggct tgaagaaccg cggtagaaat
     1801 caaaaactcg gtaccaaaaa cctgataata ttcttcgagt ttataggcga aaccaatggt
     1861 gtcgtttgat ttaatcaagc cagaaataat gaaaagaacg cctactagcg tgcggcaaag
     1921 atgaacgagg tacttcatac aggttggtca aaaattataa aaaaccggcg cggcttagca
     1981 aatatcaagc ctcatcttcc gaaattttga tgagggcaaa aatagcgtaa ttgatcatgt
     2041 ccatgtaatt ggcatcaatc ccttcagaaa tgagcgtagc gccttgatta tcttccattt
     2101 gcttgatacg catgagtcga actagaatca tatcagtaaa agaactcaca cgcatatctc
     2161 gccaagcttc accataatcg tggtttttct gcatcataag tgcttttact tcagcaacag
     2221 tttgatcata aagctgttct gaacgttcac gacttaattc caattcagaa tcggttccta
     2281 attgcatttg aatcaatccc attaaagagt aattcacgat tccaataaac tcaggttcaa
     2341 taccttcttc cacaaggtgc atacctttgg tttcgattcc tctaatgcga cttgctttaa
     2401 taaaaagctg gtcggtgata gacgaaatcc gaaaaatccg ccaagaagtg ccgtaatcgg
     2461 tgttcttttt gagaaaaata tctttgcatc gttgaatgac tgcgtcgaat tgtgccgatg
     2521 tttgttgcat ggaaaccaga atcaagggca cgaaattaac atcatccccc gaaatccaca
     2581 gcattcgcat tcaaggaaag caattttcct tcgataagcc ctgtgtaatg ggcataatta
     2641 atattaacga ggactcattt tttgctggaa gtcgtgtttc tgactcgaat ttcctccaat
     2701 tagccagtcg acatgtacaa gaaggtgctc agtttttaga tttaggtgct agttcaagtc
     2761 ggccgggagc tttaattagt caggcaagtg atgagtggaa acgtttacaa cctgttatta
     2821 ctgctgtcag aaaagagttt ccaaagagta taatttcagt ggatacgtat catgcgcaag
     2881 tagcacgttg ggcggttgaa tcgggtgcag atttgatcaa tgatatttcg gctggaaaga
     2941 ttgataatgc tatgttttcg acagttgctg aattgaaagt gccctacatt ctcatgcaca
     3001 tgcaaggccg tcccgattcg atgcagcaga atccagcgta taaagatgtt gtttatgagg
     3061 tgtttcacga attgcatcaa agcttagaag cacttaggca agtaggagta gaggatgtta
     3121 ttgttgatcc cgggtttgga tttggtaaaa gcttagagca taattatcag ttattgaata
     3181 agttggatgt gtttcacaaa ctgaaaatcc cattattggt tggtgtttcg cgaaaatcaa
     3241 tgcttactcg tttattagat gtgaaagtgg atgatgcttt gaatggatca accgttttac
     3301 atacaatagc cattcaaaaa ggggctcaaa ttttacgtgt tcatgatgta aaaccagcag
     3361 ttgaagccat tcggatattg gaaatgattc gttctgtcaa ataatagagt agtgcaggct
     3421 aagcgagctt ccttacattt gccctcatgc aattgctgat ttcctttttt ataaccctgc
     3481 gctggctcga catagtcgat attgtcattg tagccatttt actctaccaa ttgtacagtt
     3541 tggtaagagg tacaagtgca gtgagtattt tctttggaat tctcttgttt tacctcctat
     3601 ggttgctcgt taaagcgcta aatatgcagc tattagcaac cattctcgga caatttattg
     3661 gagttggagt aattgcactc attatcgtat ttcaacaaga acttcgtagg tttttattga
     3721 tggttggctc tcgtggaatc ttcaacaaag aaaacttcaa aggcttttca ggattgaatt
     3781 cggcagctta tggtagttta aactacgaat cgattgtgac tgcttgtcag aagatggctt
     3841 tacagcatac cggtgctttg atagttatca gtcgtaaatc ggatttgaag tattatgcaa
     3901 atacgggtga gcggctagat tcagagataa ctgctaatat tttggaatcg atctttttta
     3961 agaacaatcc attgcatgat ggagcagtaa ttatttcgaa taaccgtgtg aaagctg
//
LOCUS       DAOOQW010000219         3978 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_27023_length_3978_cov_5.244966, whole genome shotgun sequence.
ACCESSION   DAOOQW010000219 DAOOQW010000000
VERSION     DAOOQW010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3978
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27023_length_3978_cov_5.244966"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="PK281_10230"
     CDS             complement(<1..449)
                     /locus_tag="PK281_10230"
                     /inference="COORDINATES: protein motif:HMM:NF015747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HOY49761.1"
                     /translation="MLKPKNICIIGTGKIARHLAEKLASIADYSVCIWGRNAESAQTI
                     SQETGIKLLKDISEIPASSIGILCVSDHAIQNVAHDLIGRFELIIHLSGTSSIDLLKD
                     SAAHTAVMWPNQSFTPGNIINWKNVPLCLEASDEFAHDELSELAKNLG"
     gene            complement(436..3063)
                     /gene="ccsA"
                     /locus_tag="PK281_10235"
     CDS             complement(436..3063)
                     /gene="ccsA"
                     /locus_tag="PK281_10235"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HOY49762.1"
                     /translation="MDIQYIGEHTYWGTIGHLFISLSFAAAILSAVSYWFAQTSPNDS
                     AAWKKLGRLGFRLHSLGVVGIIGVMFFILFNHYFEFHYVWQHSNKAMQMKYILSCFWE
                     GQEGSFLLWTFWNMVLGNILIRISRDWEFSTMTIVALVQVFLASMLLGVYVGDVHIGS
                     NPFILLREHPDFVGLPFIQMPDYLAKLDGRGLNPLLQNYWMTIHPPTLFLGFSLTLFP
                     FAYAIAGIWRKQYREWIAPALPWTFFGISILGTGILMGGAWAYEALSFGGFWAWDPVE
                     NASLVPWLTLVAGGHVMLINKSKGQSVMTAIFMVFITFILILYSTFLTRSGVLGESSV
                     HAFTDLGMTGQLLIYLFFFIWLPSAITLPHGTFRTIWYTVSVGLLLVSFFFGFMKLVT
                     AIFLLIAIGIMIRQVTKALPKDEEEESISSREFWMFVGALVLLISAFQITFSTSTPVI
                     NKFLAGNLAGFFGWLNGIFSSDLFTKLADANMASPKDASLHYNAWQIPFAVIVAVLMA
                     VGQFLKYKKTPMADFYKKILLPFSLAFVLTAVIGLWMEIDKPLLLLLLFASLFATLAN
                     FHYLFVVLKFKMAKAGSSVAHVGFGLVLMGALISAGKKEFISQNKSSFDLGKDFPNQE
                     NILLARNDTVQMGEFDVSYRGDSIDGINIYYNVDYYKKNAAGQPELAFRLSPLIQTNP
                     RMGNVAEPATKHYLGRDIFTHITYAKLEENTKKKDSEWKEPQDYQLHLGDTMFTSNSL
                     VILDNIQKDIPLTTSPMLQAGDLPVGAQLRVLNVNSKTLNALPLYVLRGNTTFAIDDE
                     VEASGLKFSFWKIDPETETFHIRVSEKVSNTNDFIIMKAIIFPGINILWIGSLLMAIG
                     TGMAVWDRIKFSGRKRAQA"
     gene            complement(3087..3509)
                     /locus_tag="PK281_10240"
     CDS             complement(3087..3509)
                     /locus_tag="PK281_10240"
                     /inference="COORDINATES: protein motif:HMM:NF015085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HOY49763.1"
                     /translation="MKKIHIIGILVIALAIGMILSTFSDSSTYASFKEAMEAPNQEFH
                     VVGKLNKEKPLEYNPEQNANLFGFYLIDRDGLEQKVLFNGSKPQDFEKSEQVVITGKM
                     DGQTFKADKILMKCPSKYNNGSGDMKEFKAEDNKTTSL"
     gene            complement(3506..3730)
                     /locus_tag="PK281_10245"
     CDS             complement(3506..3730)
                     /locus_tag="PK281_10245"
                     /inference="COORDINATES: protein motif:HMM:NF040113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CcmD family protein"
                     /protein_id="HOY49764.1"
                     /translation="MSFKRILLLFALFLSSGLMAQNDVEMADTFRADGKIYVVVAILS
                     IVFTGIVLFLIRLDRKISHIEKEQRKSEKE"
     gene            complement(3720..>3978)
                     /locus_tag="PK281_10250"
     CDS             complement(3720..>3978)
                     /locus_tag="PK281_10250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49765.1"
                     /translation="LLIVFLVVYPRMNKVDSLHPGNGGNPAFSSYDLDKNMRMVFYPA
                     VFGWIMTGLWIANISIRSSIVRYKQLRKQFSRPDSNPTHVI"
BASE COUNT         1202 a          836 c          851 g         1089 t
ORIGIN      
        1 cccagattct tagctagttc agataattca tcgtgagcaa attcatcgga agcttcgagg
       61 caaagcggaa catttttcca gttgatgata tttcccggag tgaagctctg attaggccac
      121 ataacagctg tgtgtgcagc gctatccttt aaaagatcga ttgatgatgt acctgaaagg
      181 tgaatgatca attcaaagcg accgattaag tcatgtgcca cattttgtat ggcatgatcg
      241 ctcacgcaaa gaatcccaat cgagctggca ggaatttctg aaatgtcttt gagcagcttg
      301 atgcctgttt cttgcgaaat agtttgagct gattcagcat ttcgtcccca aatacaaaca
      361 gaataatcgg ctatgcttgc tagtttttcg gcaaggtgac gggcaatttt acctgtcccg
      421 attatgcaga tgtttttagg cttgagcacg tttccggcct gaaaatttga ttcgatccca
      481 aactgccata cctgttccga ttgccatgag taatgaacca atccagagaa tattgattcc
      541 cggaaagatt atggctttca taatgatgaa atcgttggta ttgctcactt tttcgcttac
      601 gcggatgtga aatgtttcgg tttctggatc aattttccaa aagctgaatt tcaatccgga
      661 agcttccact tcatcatcaa tcgcaaaggt tgtatttcct cgcaaaacgt aaagtggaag
      721 tgcatttaag gttttgctat tcacattcaa aacccgaagt tgagctccaa caggtaaatc
      781 acctgcttgt aacattggtg aagtagtaag cggaatgtct ttttgaatat tatccaatat
      841 caccaaacta ttgctggtaa acatggtatc acccaaatgc aactgataat cttgcggctc
      901 tttccactcc gaatctttct tctttgtgtt ttcttccagc ttcgcatacg tgatgtgagt
      961 gaaaatatcg cgtcccaaat aatgcttcgt agctggttct gctacatttc ccatacgtgg
     1021 attagtctga ataagcggac tcaagcggaa agctaattcg ggctgaccag cagcattctt
     1081 cttgtaataa tcaacgttgt agtaaatatt gataccatcg atagagtcac cgcggtagct
     1141 aacgtcaaac tcgcccattt gaacggtgtc gtttcgggca agaaggatgt tttcttgatt
     1201 aggaaaatct ttccctaaat cgaatgagct tttgttttgg gagatgaatt ctttcttgcc
     1261 agctgaaatc aatgcaccca tcaataccaa tccaaaccca acgtgagcca cagaagaacc
     1321 agctttcgcc attttaaact tcaagaccac aaacaggtaa tggaaattag ccaaggtggc
     1381 aaacagcgaa gcgaagagta ataatagtaa gagtggttta tcaatctcca tccataaacc
     1441 aatcacagct gtcaatacaa atgctaagga aaatggcagt aaaatcttct tgtagaagtc
     1501 ggccattgga gttttcttgt atttgaggaa ttgtccaacc gccatgagaa ccgccacaat
     1561 cacagcaaaa ggaatctgcc aagcattgta gtgcaaggat gcatctttgg gtgaagccat
     1621 gttggcatcg gccaatttgg tgaacaagtc ggagctaaaa attccattca accaaccaaa
     1681 gaatccggca agatttcctg ccaagaactt gttgattacg ggagttgagg tggagaatgt
     1741 gatttgaaat gctgaaatca acagcaccaa cgcaccgaca aacatccaga attcacgcga
     1801 actgatgctt tcttcttctt cgtctttagg caatgccttc gtaacctgac gaatcatgat
     1861 tccgatagcg atgagcaaga aaatcgctgt gaccagtttc ataaatccga agaagaaact
     1921 cactaataaa agtcccacgc ttacggtgta ccagattgtt cggaacgttc cgtgtggtaa
     1981 agtaatggct gatggcaacc aaatgaagaa gaacaagtaa atgagcaact gtcccgtcat
     2041 acctaaatcg gtgaaggcat ggacagatga ttcaccgagt acgccgcttc gggtaaggaa
     2101 agtagaatag aggataagga taaacgtaat gaaaaccatg aaaatagccg tcatcactga
     2161 ttgtcctttg gatttgttga tgagcatgac gtgtccgcca gcaaccaaag tgagccaagg
     2221 aacaagagaa gcattttcaa ccggatccca agcccagaaa ccaccaaagc ttaacgcttc
     2281 atatgcccaa gcgccaccca ttaaaatccc agttcctaag atgctgattc cgaagaaagt
     2341 ccaaggaagt gctggagcta tccattcgcg gtattgtttt ctccaaattc cggcaatggc
     2401 atatgcaaac gggaatagcg tgagagagaa tccgaggaat agggtaggag gatgaatcgt
     2461 catccagtaa ttctgaagca aaggatttaa accgcgccca tcgagtttag ctagatagtc
     2521 gggcatttga atgaatggaa gtccaacaaa atcgggatgt tcgcgtagaa ggatgaatgg
     2581 attactacca atatgcacat cacccacata cacacccaaa agcatagatg ccaaaaaaac
     2641 ttgtacaagt gccacaatag tcatggtact aaattcccaa tcgcgactga tgcgaatgag
     2701 gatgtttcca agtaccatat tccagaatgt ccagagaagg aaactacctt cttgtccttc
     2761 ccagaagcag gaaaggatgt atttcatctg catggctttg ttactatgct gccacacata
     2821 atggaattcg aaatagtggt taaataggat gaaaaacatc accccgataa tccctacaac
     2881 acctaatgaa tgcaagcgaa agccgaggcg accgagtttc ttccaagctg ccgaatcgtt
     2941 cggacttgtt tgtgcaaacc aataagatac cgccgataaa atagctgcag caaaagaaag
     3001 ggaaataaac aggtgaccga ttgtccccca ataagtgtgt tctccgatat attgaatgtc
     3061 catccgcgct atcctcctgt tttaaattac aaggaggttg ttttgttatc ttctgctttg
     3121 aattccttca tatcgccact gccgttgttg tatttacttg ggcatttcat gaggatttta
     3181 tcggctttga aggtttgtcc gtccatttta cctgtgataa cgacttgttc tgatttctca
     3241 aaatcctgag gtttagaacc gttgaacaga actttttgtt cgagtccatc gcgatcgata
     3301 aggtagaatc cgaacaggtt ggcgttttgt tcgggattgt attcgagcgg tttttctttg
     3361 ttcagttttc ctaccacgtg gaattcctga ttaggagctt ccatggcttc tttgaaagaa
     3421 gcgtaagtgc tcgaatcgga gaatgtactg agtatcatac caattgctaa tgcaatgacg
     3481 agtataccga taatgtgaat ctttttcatt ctttttcaga tttacgctgt tctttttcga
     3541 tgtgactgat tttgcgatcg agacggataa ggaaaagtac aatgcctgta aacacgatag
     3601 atagaattgc aactaccacg tagattttac catcggcgcg gaacgtatca gccatttcta
     3661 catcattttg agccattagc ccagaactta gaaacaaagc gaataaaaga agtatgcgtt
     3721 taaatgacat gagttgggtt actatctgga cgtgaaaact gtttgcgtag ttgcttgtag
     3781 cgcacaatgg atgagcggat ggagatattg gcaatccaca atccggtcat aatccaacca
     3841 aaaacagctg gatagaaaac catgcgcata tttttatcga gatcgtacga gctgaatgca
     3901 ggatttccgc catttccagg atggagtgaa tcaactttat tcatacgcgg atacacaacc
     3961 aagaatacaa ttaacagc
//
LOCUS       DAOOQW010000220         3973 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_27077_length_3973_cov_4.766718, whole genome shotgun sequence.
ACCESSION   DAOOQW010000220 DAOOQW010000000
VERSION     DAOOQW010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3973
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27077_length_3973_cov_4.766718"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="PK281_10255"
     CDS             complement(<1..536)
                     /locus_tag="PK281_10255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49766.1"
                     /translation="MKYTWIISLVFVSLSTQIYAQISDYFAGNPHWRINSSCSAPYPC
                     VRDDQAEYVVVADTLMDSILWHKIQRQGISSYTYFNSPPITGECSGTFDFSEQAGFLR
                     QDSMKIYFRTLVNSTELLVYDFDLEVGDSLPITTINFQDFLVVNAVDSVLIGSTWRKR
                     IYFNEGTSWLIEGLGHNGG"
     gene            complement(564..1016)
                     /locus_tag="PK281_10260"
     CDS             complement(564..1016)
                     /locus_tag="PK281_10260"
                     /inference="COORDINATES: protein motif:HMM:NF039391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6265 family protein"
                     /protein_id="HOY49767.1"
                     /translation="MTFFLLVGETEIEQKQAFDAFKVCEGDWVFQSKEGEFHESWNYV
                     NDSLFIGNGCFIQQGDTVFSEKIRVIYSNDKMYYCALDANQNSGAEVCFPLKKFTGKR
                     WEFELKEHDFPQLIGYEKPQSDSLFAWIEGTVKEKFRKNEFSFKRISK"
     gene            complement(1040..3112)
                     /locus_tag="PK281_10265"
     CDS             complement(1040..3112)
                     /locus_tag="PK281_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015023845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 protein"
                     /protein_id="HOY49768.1"
                     /translation="MKQSILIILSALILVSCSEEKKRYSIRLFSPSHKTSISFLLSPG
                     GTPGYCVQYKDQIIVDSSSLGFELKETAPIKSDFEMIGNHLDSLNENWEQPWGEQRIV
                     NNHYNELTVNLREKIEPHRLMNLIFRSYDDGVAFRYEFPDQEAMREMTILEENTKFRL
                     KEDYTCWWAPGDWDITEHLYTNSLLSKIDATKKRNQPSLGQTYIPDNAVNTPLTMRSK
                     DGLHISIHEAALVNYSDMTLKVDTANLQLQSSLVGSDRLGYKAKVQVPFKTPWRMILL
                     SPNAAGLVDSRMILNLNEPSKVKDVSWFKPMKYTGIWWEMHLGKSSWDMASGKHGATT
                     ANAKKYIDFTAKNGLGGVLVEGWNTGWEHWIGFDDREGVFDFVTPYADYNLEEVVQYA
                     HSKGVEIIMHHETSSAPQTYEKQLDKAYALMKRLGLHTVKTGYVGKILPKGEYHHGQW
                     MINHYQKVIETAAKYQIAVDAHEPIKGTGLQRTWPNFVSREGMRGQEFNAWSSDGGNP
                     PDHLPNVVFTRMLSGPFDFTPGVFNIKLKPYKDKNQVSTTLAQQLALYVVIYSPVQMA
                     CDLIENYEGQPGFQFIRDVGTDWESSKTLAGEVGDFVTIVRQEKKTGNWFLGSITDEN
                     ARDISVDFSFLPKGIEFTADVYKDGANAHWDSNPTSLQIDHVKINSTTKLDFHLAPGG
                     GLAIAIKK"
     gene            complement(3224..>3973)
                     /locus_tag="PK281_10270"
     CDS             complement(3224..>3973)
                     /locus_tag="PK281_10270"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOY49769.1"
                     /translation="ASQSLEEHFKKVILDAMATYDVSLVAIASTILRDTTLPGNSPSI
                     FPIPSRDIKFLEDALNQMRLPRDIESYGEILKTIRFFEGNPIEGNLKPEMNNPINWQL
                     VERIPDNQQIEIHTDAGIIRAELWVNDAPGTVANFIHLIQDSFYINKRLHRVVPGFVI
                     QDGCPRGDGYGSRMETIRSEFSSRNFDEGILGMASAGPDTESCQWFITHTRTPHLNGR
                     YTAFGKVVDGMDVVHKLGIGAKIQRIELLAP"
BASE COUNT         1245 a          814 c          639 g         1275 t
ORIGIN      
        1 cctccattat gtcctaatcc ttcaattaac catgatgttc cttcattaaa ataaattctc
       61 tttctccacg tcgagcctat taaaacacta tcaactgcgt ttacaactaa aaagtcttgg
      121 aaatttatgg ttgttatagg aagtgaatca cctacttcta aatcaaaatc gtaaactaac
      181 agttcggttg aattaactaa tgttctaaaa taaatcttca tactatcctg acgaagaaag
      241 cctgcttgct ctgaaaaatc aaaggttcca ctgcattctc cagtaatggg aggtgaatta
      301 aaataggtat aacttgaaat tccttgtcgt tggattttat gccatagaat actatccatt
      361 aaggtatcag caactacaac atattcggcc tggtcatcac gaacacatgg ataaggagca
      421 ctacatgagc tattgattct ccaatgtgga ttgcccgcaa aataatcgct tatttgggca
      481 tatatttgag tgctaagact aacaaataca agactaataa tccaagtata cttcataacg
      541 aaagtttaca ttaaatataa tgttcatttc gatatacgct taaaagaaaa ttcgtttttc
      601 ctaaatttct cttttacagt accttctatc catgcaaaca gagaatctga ttgtggtttc
      661 tcatatccga ttaattgtgg aaaatcgtgc tcttttaatt caaattccca tcgcttacct
      721 gtgaatttct tcaaaggaaa acaaacttct gctccactat tttgattcgc atctaaagca
      781 cagtaataca tcttatcatt cgagtaaatc actcgaattt tctcactaaa tacggtgtca
      841 ccctgttgaa taaaacaacc atttccaata aataaactgt cattcacata gttccaagat
      901 tcatgaaatt caccttcttt cgattgaaat acccaatcac cttcacaaac tttaaaagca
      961 tcaaatgctt gtttttgttc tatttcggtc tcaccaacca atagaaagaa agtcaaaaca
     1021 aaaattgata aagactgaat catttcttaa tcgcaatagc taatcctcca ccaggagcta
     1081 aatgaaaatc aagtttagtt gtggaattaa tcttcacatg atcaatttgc aaactggttg
     1141 gattgctgtc ccaatgcgca tttgctccat ctttatacac atccgcagta aactcaattc
     1201 ctttaggtag aaaactaaaa tcaaccgaaa tatcacgtgc attttcatcc gtaatcgaac
     1261 ccaaaaacca attcccagtt ttcttctctt ggcgaacaat cgttacaaaa tcacccactt
     1321 cgcctgctaa tgttttggag ctttcccaat cagttcctac atcacgaata aattgaaagc
     1381 cgggttgtcc ttcataattc tctattaaat cacaagccat ttgaactgga ctgtaaatta
     1441 ccacatacag tgctaattgt tgagcaagtg ttgtactaac ttgatttttg tctttataag
     1501 gcttcaactt aatattgaaa actccgggag taaaatcaaa cggtccgctc aacatacgtg
     1561 taaaaaccac atttggtaag tgatcgggag gatttccacc atcgctcgac catgcattaa
     1621 attcttgacc acgcattcct tcgcgagaaa cgaaattggg ccaagtacgc tgcaatccag
     1681 tacctttaat gggttcatgt gcatctacgg caatttgata tttcgcagcc gtttcaatga
     1741 ctttctgata atgattaatc atccattgtc catgatgata ttcgccctta ggtaaaattt
     1801 taccaacgta tccagtttta actgtatgca atcccaaacg tttcatcaag gcatacgctt
     1861 tatcaagttg cttttcgtaa gtctgtggag ctgatgatgt ttcgtgatgc atgattattt
     1921 caacaccttt tgaatgcgcg tattgaacaa cttcttccaa gttataatcg gcatacgggg
     1981 tgacaaaatc gaaaactcct tctcggtcat caaaaccaat ccaatgttcc caaccggtat
     2041 tccatccttc taccaaaact ccacctaggc cattttttgc agtaaaatcg atgtattttt
     2101 tagcattggc ggttgttgca ccatgttttc cgctagccat atcccaagat gatttcccta
     2161 agtgcatttc ccaccaaatt ccggtgtatt tcatgggctt gaaccaagaa acatctttca
     2221 ctttactagg ctcattcaaa ttcaatatca tgcgcgaatc aactaagcca gcagcattgg
     2281 gactaagtaa aatcatgcgc caaggtgttt taaaaggcac ttgaactttt gctttatatc
     2341 ctaatcgatc cgaaccaacc aaagaacttt gtagttgaag attggcagta tcaaccttaa
     2401 gtgtcatatc ggaataattc accaatgctg cttcatgaat actgatgtgc aaaccatctt
     2461 tagaacgcat ggttagtggc gtattgactg cattatcggg aatatacgtt tgacctaaac
     2521 ttggttgatt ccttttttta gttgcatcaa ttttagataa aagactatta gtatataaat
     2581 gttcggtgat atcccaatcg cccggagccc accaacaggt ataatcttct ttcaaacgaa
     2641 atttggtatt ttcttcgaga atggtcattt ctcgcatggc ttcttgatca gggaattcat
     2701 aacgaaaagc aactccatca tcatacgatc taaatattag attcatcaat cgatgcggct
     2761 caattttctc tctaagatta acagttagtt cgttataatg attatttacg atgcgttgtt
     2821 ctccccaagg ttgttcccaa ttttcattga gtgaatcaag gtgatttcca atcatttcaa
     2881 aatccgactt aattggtgca gtttctttta actcaaatcc taatgaggaa ctatcaacaa
     2941 tgatttgatc tttgtattga acacaatagc caggagttcc gccagggctc aataaaaatg
     3001 aaatacttgt tttatgggaa ggagaaaata atcgaatact atatcgcttt ttttcttcgg
     3061 aacaacttac tagaattaaa gcagaaagta tgattaggat tgattgtttc attggaaaaa
     3121 ctttatagaa aattgttcga agcattgtgt ggacttcaat tatttcagcg aatataagga
     3181 ttcattactg gaagagaatt aaggtgaata gtgctaagga attttaaggt gcaagtaatt
     3241 ctattcgctg aattttagca ccaatcccta gtttatgcac tacatccatt ccatctacca
     3301 cttttccaaa agctgtatag cgaccattta aatgaggcgt tcgggtatgt gtaataaacc
     3361 actgacaact ttctgtgtcg ggcccagcac ttgccattcc taaaatgcct tcatcaaaat
     3421 ttctacttga aaattcacta cgtatggttt ccatgcgact tccataacca tctccgcgcg
     3481 gacatccatc ttgaatcaca aaacctggaa caacacgatg caaacgttta ttgatataaa
     3541 agctatcttg aatgagatga ataaaatttg ctactgttcc tggggcatcg tttacccata
     3601 attcagcccg aataattcct gcatcagtat gaatttctat ttgttgattg tctggaattc
     3661 gttcaactaa ttgccaatta attggattat tcatttcggg ttttaaattt ccttcaatgg
     3721 gatttccttc gaaaaatcga atggttttta agatttcccc ataggattca atatcacgag
     3781 gtaagcgcat ttgatttaat gcatcttcca gaaatttaat gtctctggat ggaataggaa
     3841 aaatacttgg tgaattacct ggtaaagtag tatcacgaag tattgtagat gcaatagcga
     3901 caaggcttac atcataagta gccatcgcat ctaaaataac ctttttaaaa tgttcctcga
     3961 gcgattgtga agc
//
LOCUS       DAOOQW010000221         3885 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_27858_length_3885_cov_4.580940, whole genome shotgun sequence.
ACCESSION   DAOOQW010000221 DAOOQW010000000
VERSION     DAOOQW010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3885
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27858_length_3885_cov_4.580940"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..521
                     /gene="nth"
                     /locus_tag="PK281_10275"
     CDS             <1..521
                     /gene="nth"
                     /locus_tag="PK281_10275"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOY49770.1"
                     /translation="SSDEVFSYIRSISYPNNKAKHLVGMAKMLVQDFHNQVPSDVDEL
                     VKLPGVGRKTANVIASVVYDLPAMAVDTHVFRVSARLGLTRGAKNPLQAEKQLVKHLP
                     DAIIPKAHHWLILHGRYICTARKPNCAQCGLTSICLFYAKKQQSEIVKPVLSKPTEQK
                     VKRRVSSKPIKN"
     gene            527..976
                     /gene="bcp"
                     /locus_tag="PK281_10280"
     CDS             527..976
                     /gene="bcp"
                     /locus_tag="PK281_10280"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007651142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HOY49771.1"
                     /translation="MATLKVGDKAPEIQANDQHGKPVSLKEYAGKQVILYFYPKDDTP
                     GCTAEACNLRDNYDDLLKQGFDVIGVSPDEEKKHLKFIEKYQLPFRLVADTDKKVAEA
                     YGVWGLKKFMGKEYMGVNRTTFVIDVDGNIGKIIDKVETKNHAAQII"
     gene            1113..2123
                     /gene="recA"
                     /locus_tag="PK281_10285"
     CDS             1113..2123
                     /gene="recA"
                     /locus_tag="PK281_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010516196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOY49772.1"
                     /translation="MSKDIQSDKLKALQVIIDKIDKTYGKGTIMKMGDSPIESMEVIH
                     TGSISLDLALGVQGFPKGRIIEIYGPESSGKTTLAIHAIAQSQKAGGIAAIIDAEHAF
                     DRYYAEKLGVDVENLLISQPDNGEQALEIADNLIRSGAIDIIVIDSVAALTPKSEIEG
                     EMGDSKMGLQARLMSQALRKLTANISRTGSTCIFINQLREKIGVMFGNPETTTGGNAL
                     KFYASIRLDIRRIGQIKDGEAVVGNRTRVKVVKNKVAPPFRTAEFDIIYGEGISRIGE
                     IIDLGVDNNIIKKSGSWFSYEDTKLGQGRDAVKTLLGDNPELMEEIEGRIREAIAGPV
                     EA"
     gene            2247..3251
                     /locus_tag="PK281_10290"
     CDS             2247..3251
                     /locus_tag="PK281_10290"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOY49773.1"
                     /translation="MKLRNLPFLFFAVAIFFSWVITSCGGKPENDLTTPAGIAAALAE
                     DSTNPEALHARARMYITRNRPDSALTDMLKAIARDSSVSGYFVTLGDAYLMVNQTRYT
                     RQALERAIRLEPDNKDAHMKLAELFLYVEMRQEAINEVNEVLKIDRNNPKGYYLKGII
                     YKESGDTSLAVSSFMTTIEQDPKYVLAYEQIGLIYAGARNPRAIDYYQSALRLDPKNS
                     LTRYNLGMFYQETGQIDKAMETYQELVELDKSFPNGYYNMGFILFEYKNDIKAALPMF
                     EKAASAGGKYPAAVYMIGVCQEKLGNKEQAIANYRQVLILDSRLETAREGLIRLGAKP
                     "
     gene            3317..>3885
                     /locus_tag="PK281_10295"
     CDS             3317..>3885
                     /locus_tag="PK281_10295"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOY49774.1"
                     /translation="MLSLTLPLILINAVVSISAFSKSEIMARYQFNAYMIYHRKQWYR
                     FITHAFLHADWMHLIVNMFVLYTFGQFVEMNLAYHFGSISPLIYLLLYFGAIIVSSIS
                     TYRKHKEDHWYNAVGASGAVSAVMFSSVVFGPFMKIYLYGILPLPAIIWAFIYVGYSV
                     YMSNRASDNINHDAHLWGGFYGMFLTLVL"
BASE COUNT         1177 a          737 c          816 g         1155 t
ORIGIN      
        1 cttcatcaga tgaagttttt tcttacataa ggtcaatctc gtatcccaac aataaggcga
       61 agcatttagt cggaatggcg aagatgttag ttcaagattt tcataatcaa gttccttcgg
      121 atgtagatga attagtgaag cttcccggcg taggccgaaa aacagccaat gtgatcgctt
      181 ctgttgtgta tgatttacct gccatggctg ttgatacaca tgtgttccgg gtttctgccc
      241 gtctgggatt aacacgcggc gcaaaaaatc cactccaagc tgagaagcaa ttggtcaaac
      301 acttacccga tgctatcatc ccgaaagcac atcactggtt aattcttcac ggtcgttata
      361 tatgtactgc ccgaaaaccc aattgtgctc aatgtggatt aacttctatc tgtctctttt
      421 acgctaaaaa acagcagtcg gaaattgtaa aaccggtctt atctaaacca accgaacaaa
      481 aagtaaaacg acgtgtttct tcgaaaccaa taaaaaactg aacccaatgg caacattaaa
      541 agttggcgac aaagcccccg agatacaagc aaatgatcaa catggaaagc ctgtttcatt
      601 aaaggaatat gctggaaaac aggtaatttt atacttctat cctaaagatg atactcctgg
      661 ttgcactgcc gaagcgtgta acttaagaga taattacgat gatttattga aacaaggttt
      721 tgacgtgatt ggtgtcagtc cggatgaaga gaaaaaacac ctgaaattca tcgagaaata
      781 tcagcttcca tttcgtttag tagctgatac agataagaaa gtagccgaag cttatggtgt
      841 ttggggattg aaaaaattca tgggaaaaga gtacatggga gtgaatcgga cgacttttgt
      901 aattgatgtt gacgggaata ttggcaagat aatcgacaag gtggaaacaa aaaatcacgc
      961 agctcagatt atttaaataa ttgtatatca attaaataac aggtttttca acaataatgc
     1021 tgaaaacatt tccgaattta ttttaaaata atcgtgcgcc gcattctcct tttgacgtaa
     1081 tttgtggcaa actaaccgtc aaatcatttc acatgagcaa agacattcaa tccgataaat
     1141 taaaggcact ccaagttatc attgataaaa tcgataagac atacggaaaa ggaaccatta
     1201 tgaagatggg tgattctccg attgaatcga tggaagtcat acatacagga tctatatcat
     1261 tagatttagc tttgggtgtt caaggatttc cgaaaggaag aatcattgag atttatggtc
     1321 cggagtcttc gggtaaaacc acgctagcca ttcacgctat agcacaatcg caaaaagctg
     1381 gaggaatagc tgctatcatt gatgcggaac atgcatttga tcggtattat gctgagaaat
     1441 tgggagttga tgtagaaaat ctattgatct cacaacctga taatggagaa caagctttag
     1501 aaattgccga taacctgatt cgttcagggg cgatagatat tatagtaatt gactcagttg
     1561 cggctcttac tccaaagagt gagattgaag gcgaaatggg cgattctaaa atgggcttac
     1621 aagcacgttt gatgtctcag gctttgcgta agctaacggc taatatttcc cgtactggta
     1681 gtacctgtat tttcatcaat cagttgcgtg agaaaattgg tgtgatgttc ggtaatccgg
     1741 aaacaacaac tggtggtaat gctcttaagt tctacgcttc tatccgtttg gatattcgtc
     1801 gtatcggaca aattaaagat ggtgaagctg ttgtcggaaa tcgtactcgt gtgaaagtgg
     1861 tgaagaataa agttgcacct cctttccgta cagctgagtt cgatattatt tatggagaag
     1921 gaatttctcg tattggtgag attatcgact taggtgtaga caacaacatt attaagaaaa
     1981 gtggttcttg gtttagttat gaagacacga aacttggtca aggtcgtgat gctgtgaaaa
     2041 cactattagg cgataatccc gaattgatgg aggaaataga aggtcgcatc cgtgaggcaa
     2101 ttgccggacc ggtagaggct taacatcagc acatttctca ttttaataag ccctgcaaag
     2161 acttaacagt tatttgcagg gcttttttct gacggaacat caaaaatgtt tctaaatcaa
     2221 gcctcatctt tctgtttctt tgttccatga agctgagaaa ccttccattc cttttttttg
     2281 cagttgctat tttcttcagt tgggtaatta cttcttgtgg aggtaaaccc gagaatgatc
     2341 taactactcc agctggtatc gcagcagctc ttgccgaaga ctcgaccaat ccagaagcat
     2401 tacatgcgcg tgctcgaatg tacattacgc gtaatcgtcc cgactcggca ttaaccgata
     2461 tgctcaaagc catcgctcgt gactcgagtg tgtcaggtta tttcgtgact ttaggagatg
     2521 cctatctaat ggtgaatcaa acacgctata ctcgacaagc tctagaacga gctattcgat
     2581 tagaacccga taataaagac gctcacatga aattagctga gttgtttttg tatgtcgaaa
     2641 tgcgtcaaga ggctattaat gaagtgaatg aagtactaaa aatcgatcgt aataatccta
     2701 aagggtatta cctcaaagga atcatttaca aagaatcggg cgataccagt ttagctgtat
     2761 ccagcttcat gactaccatc gaacaagatc caaaatacgt gttagcatac gaacagattg
     2821 gactaatcta cgcaggcgca agaaatccgc gagcaataga ttattaccaa tcggcactta
     2881 ggcttgatcc taaaaattcc ctcacacgtt acaatttagg aatgttctat caggaaactg
     2941 gacaaataga taaagccatg gaaacctatc aagagttggt cgaattggac aaatcttttc
     3001 ccaatggata ctacaacatg ggtttcattt tgtttgaata taaaaatgac atcaaggcag
     3061 cacttcccat gttcgaaaaa gctgcaagtg ctggtggtaa ataccctgca gctgtttata
     3121 tgattggtgt ttgtcaggaa aagctgggta acaaagaaca agctattgca aattatcgtc
     3181 aagtacttat tcttgattct agattagaaa ctgcacgcga aggtctaatt cgattaggtg
     3241 caaaacccta agttttaaca cagttaaaat ttctcatctt tccgtataca cggtaaggct
     3301 tatctatctt tgcaccatgc tttctctcac tctgcctctg attctgatca atgctgtcgt
     3361 atctatttcg gcttttagta aatccgaaat catggcgcgt tatcagttca atgcgtatat
     3421 gatttaccac cgtaaacaat ggtatcggtt tatcactcat gcttttctgc atgccgattg
     3481 gatgcatttg attgtcaata tgttcgtgtt gtacacgttt gggcagtttg tggaaatgaa
     3541 tctggcatat cactttggat ccatttcacc tctcatctat ctgctcttgt attttggagc
     3601 tatcatcgta tcttcaataa gcacttatcg caagcacaag gaagatcatt ggtataatgc
     3661 tgtgggagcc tctggtgctg tatctgctgt tatgttttcg agtgtggttt ttggaccatt
     3721 tatgaaaatc tacctgtatg gtattctgcc acttcctgct atcatatggg catttatcta
     3781 tgtgggttat agcgtttata tgagcaatcg tgcaagcgac aatatcaacc acgatgcgca
     3841 tctctggggt ggattctatg gaatgtttct cacgctggta ctcag
//
LOCUS       DAOOQW010000222         3882 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_27888_length_3882_cov_4.494382, whole genome shotgun sequence.
ACCESSION   DAOOQW010000222 DAOOQW010000000
VERSION     DAOOQW010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3882
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27888_length_3882_cov_4.494382"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /gene="prmA"
                     /locus_tag="PK281_10300"
     CDS             <1..613
                     /gene="prmA"
                     /locus_tag="PK281_10300"
                     /inference="COORDINATES: protein motif:HMM:NF001785.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006479 - protein methylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HOY49775.1"
                     /translation="PQNWNAIWEAQFDPIRVNNRCVIRAPFHPATNDGSIELVIEPKM
                     SFGTGHHATTRLMCLGLMELEVKGKRVMDMGCGTGVLGILAAKLGAVQVEGIDIEEWA
                     VENSIENASRNGVEMHIELGGAEKLPTEKTYDIFLANINRNILLQDMDFYVATLNSGA
                     TILFSGFLEPDVPALKEACSNRGLVFKEEKAEDNWRCLIFNTL"
     gene            708..3080
                     /locus_tag="PK281_10305"
     CDS             708..3080
                     /locus_tag="PK281_10305"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HOY49776.1"
                     /translation="MRSFLLALFCAFLVSAFPAGTFAQSAKKLIKEARSLSETNDFKG
                     AIDKLNQALQVEPTNGDAIFLRGTIYRKQENYPQALSDLKAAAELFPKSVVVLEELAL
                     SQIGANNNQDALITLDRMIGLSAKTSRFYHLKEETQIKTKNYEGVIQTGTAALAVEKE
                     DDYTYYQMARAVDLLGNTGLAENYYTKALAIALDTKGKRDNPQLLYPYYCGLADVQGK
                     LSKDDQAISNFSLAIKFNANDEQVLIKRGIIYSHKKEYQNAIQDFNQAISIQAKSATA
                     FYQRGIVNQLLGQFAAAINDFNQAILIDAKQSDYFASRAECYLRSNSYADAVRDYKQA
                     ISLNPENKDLVKLLADAKQKNFDANKETNNPVITIMQPKGIEKEITVRADAKTLQIQG
                     VVTDASWIRSLVVDGQSLKLAEEVINPEFSCSIDLKDKNGFKIQVTDAYLNTSTYEYT
                     ITRTEVGIPEVQFSKPFQTLERELFIENTASIMLEGRIRDESLIKSIIINGTSASFPL
                     EMKNPPFQALVNIGTKDTLVVHITDVLGNELITAYKLIREDTSGSNPMGTTWVVFIEN
                     SNYKNLQKLEGPGRDINDMKTALSNYKVDKIVHKKDATKADLDKFFSIELRDQIQKNR
                     VKSLIIWYAGHGKFLNETGYWIPVDGDNYDEYTYYSVNQLRASMQAYTMLKHVLVISD
                     ACESGPAFYMAMRDDMEPRLCDDWEVTKFRSAQVITSANKELSSDNSLFTTAFANTLN
                     NNPNDCISVETIALKVMGIVKQNQRQTPKFGKIKGLDDENGSFFFIKRKF"
     gene            3196..>3882
                     /locus_tag="PK281_10310"
     CDS             3196..>3882
                     /locus_tag="PK281_10310"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HOY49777.1"
                     /translation="MKTLLFHYSKLLSFIFAIALLASCSSCNKDCFAVKGKGDLVSKT
                     YSTNSFTSIHSAVEAHVFLTQGPVQSIEIKGQQNILDILDVKVDDGELLIGFENHCGS
                     INYDQLQVYITVPTIQAVRISGSGVFDATNTLNVESLNFHISGSARMSATVNATSKIE
                     GNISGSGELYLAGTCPTEKFTISGSGSIFAYGLQSSQSTINVSGSGDAEISVSDHLDV
                     TISGSGNVHYK"
BASE COUNT         1257 a          767 c          772 g         1086 t
ORIGIN      
        1 gcctcaaaac tggaatgcta tctgggaagc gcaattcgat cccattcggg tgaataaccg
       61 ctgtgtgatt cgagcaccgt ttcatccagc aaccaacgat ggaagcatcg aactggtgat
      121 tgaacccaaa atgtcgtttg gaaccggaca tcatgccacc actcgcttaa tgtgtttggg
      181 tttgatggaa ctggaagtga aaggaaaacg ggtgatggac atgggttgcg gaacgggtgt
      241 tttaggaatt cttgctgcta aattgggtgc tgtccaagta gaaggaatcg acattgaaga
      301 atgggcagta gaaaactcca ttgaaaatgc gtctcgaaat ggtgttgaaa tgcacatcga
      361 actaggcgga gccgaaaaac ttccgacaga aaaaacctac gatattttcc tagctaacat
      421 caatcgcaat attctgcttc aagacatgga tttctatgtt gcgactttaa actcaggtgc
      481 tacaatcctt tttagcggtt tccttgaacc agatgttcct gctttaaaag aagcttgtag
      541 taatcgagga ttagttttca aagaagaaaa agcggaagat aattggcgat gtttaatctt
      601 caacacactg taatcgaaca tgctacaatc ttaatatatt ttgccttcca gattaaaaag
      661 tgctaaactt tcatacattt gatataaccc attctaacac tacacctatg cgctcctttc
      721 tacttgctct attctgcgct ttcttggttt ctgctttccc cgctggtaca ttcgctcaat
      781 ctgccaaaaa acttattaaa gaagctcgca gcctttctga aacaaacgat ttcaaaggtg
      841 ctatcgataa attaaatcag gcattacaag tggagccaac gaatggcgat gctatttttc
      901 tgcgaggaac gatttatcga aaacaagaga attatcctca agcattaagc gatttaaaag
      961 ctgccgccga actattccca aaaagtgtgg tggttttgga agaattagct ttaagtcaaa
     1021 ttggtgctaa taacaatcaa gacgcactta ttacgctaga tcggatgatt ggtttaagtg
     1081 cgaaaaccag tcgtttttac caccttaaag aggaaaccca aatcaaaaca aagaactacg
     1141 aaggggttat tcaaacggga actgcagctt tagcggttga aaaagaggac gattacacct
     1201 attatcaaat ggctcgtgct gtcgatttac taggcaatac tggactagcc gagaattatt
     1261 acacaaaagc acttgctatt gcactcgaca ccaaaggcaa acgcgataat cctcaattac
     1321 tttatcctta ctactgtgga ttggctgatg ttcaagggaa actttcgaaa gatgatcagg
     1381 caatcagcaa tttcagttta gctataaaat ttaacgcaaa cgatgagcag gttttaatca
     1441 aacgagggat tatttactcg cacaaaaaag aatatcaaaa cgccattcag gattttaatc
     1501 aagccatcag catacaagca aaaagcgcca cagccttcta tcagcgtggt atagtaaatc
     1561 aattattagg acagtttgct gccgcgatta acgatttcaa tcaagccatt ttgattgatg
     1621 caaaacagtc ggattatttt gcttcacgtg ctgaatgtta tttaagaagt aactcatacg
     1681 cagatgcggt gagagattac aaacaagcca tttcactcaa tcctgaaaac aaagatttag
     1741 tgaaactttt agccgatgct aaacaaaaga attttgatgc gaataaggaa acaaataatc
     1801 cagttatcac catcatgcag cctaaaggaa ttgaaaaaga aattactgtt agggctgatg
     1861 ctaaaacatt gcaaattcaa ggtgtcgtga ccgatgcaag ctggattcgt tcattagtag
     1921 tagatggaca aagtctcaaa cttgccgaag aagttattaa tcctgaattt tcatgcagca
     1981 ttgatttgaa agataaaaac gggttcaaaa ttcaagttac cgacgcctac ctcaacactt
     2041 ccacatacga atacaccatt acacgcaccg aagtaggaat tccagaagtg caattctcca
     2101 aaccttttca aactttggag cgcgaattat ttatcgaaaa cacagccagc attatgttgg
     2161 aaggtcgcat tcgtgatgaa agcctcatca aatccatcat catcaacgga acttccgcca
     2221 gctttccatt ggaaatgaaa aatcctcctt tccaagcatt ggtgaatatt ggaacaaaag
     2281 acactttagt agttcacatc acagatgtac taggaaatga attgatcacc gcctataaac
     2341 tcattcgcga agacacaagt ggatccaatc ctatgggaac gacttgggta gtttttatcg
     2401 aaaatagcaa ctacaaaaac ctacaaaaac tcgaaggtcc aggccgcgat attaacgata
     2461 tgaaaacggc tttgtcgaac tataaagttg acaaaatcgt ccataagaaa gatgcaacca
     2521 aggccgattt agacaaattc ttctccattg aattacgtga tcaaatccag aaaaatcgag
     2581 ttaagtcgct aattatctgg tatgccggac atggtaaatt cctcaatgaa acgggttatt
     2641 ggattcctgt tgatggtgat aattatgacg aatacacgta ttacagcgta aatcagcttc
     2701 gtgcgtctat gcaagcatac accatgttga aacacgtatt ggtaatttcc gatgcatgcg
     2761 aatctggtcc agcattttac atggccatgc gcgacgatat ggaaccacgt ttatgcgacg
     2821 attgggaagt aactaaattc cgttcggctc aggtaattac atccgccaac aaagaacttt
     2881 ctagcgataa tagcttattc acgacagctt ttgccaatac cttaaataac aatccgaatg
     2941 attgtatttc ggtagaaaca attgcgctca aagtgatggg aattgtaaag cagaatcaac
     3001 gtcaaacgcc taagtttggt aaaatcaaag gcttggatga tgaaaatgga agcttcttct
     3061 tcatcaaacg taaattctga ttttattttc ctgatttaga gcattttgaa ctattatttc
     3121 aattttagcc tacaagctga taggggtaat ttcatcccga cgggtctttg tgaaaagtca
     3181 cataaatcac gtcgtatgaa aaccttactg tttcattatt ctaaacttct cagtttcatt
     3241 tttgccattg ctttattggc ctcttgctct tcctgtaata aagattgttt tgcagtaaaa
     3301 gggaaaggag atttagtgag taaaacgtat tcaaccaatt cgtttacctc aattcattca
     3361 gctgttgaag cacatgtgtt tttaacacaa ggtccagttc agtcaattga aataaaggga
     3421 caacaaaaca tcctcgatat tttagatgta aaagtggatg atggcgaatt actcattgga
     3481 tttgaaaatc attgcggaag tatcaattac gatcagttgc aagtttatat cactgttcct
     3541 accattcaag cggtgcgcat aagtggttcg ggtgttttcg atgcgacgaa tactctgaat
     3601 gtggaatcgc tcaacttcca tatttctggt tcggctcgta tgagtgcaac ggtaaatgcg
     3661 acctctaaaa ttgaaggaaa cataagtggt tcaggtgaac tttatttagc tggaacttgt
     3721 ccaaccgaaa agttcaccat ctcaggttca ggtagcattt tcgcgtatgg cttgcaatcg
     3781 agtcaatcaa ccattaatgt gagtggctcg ggcgatgccg aaatatccgt aagcgatcac
     3841 ctcgatgtaa cgatcagtgg cagtggaaac gttcactaca aa
//
LOCUS       DAOOQW010000223         3865 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_28067_length_3865_cov_5.253281, whole genome shotgun sequence.
ACCESSION   DAOOQW010000223 DAOOQW010000000
VERSION     DAOOQW010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3865
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28067_length_3865_cov_5.253281"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="PK281_10315"
     CDS             complement(<1..272)
                     /locus_tag="PK281_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020213433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3050 domain-containing protein"
                     /protein_id="HOY49778.1"
                     /translation="MDYSEQLKLAIESNRLENIHHSVFKQMKRMDDVKLFMQFHVYAV
                     WDFMSLLKTLQQELTCTSIPWFPKGDAEIRYLINEIVIGEESDIDSS"
     gene            complement(368..766)
                     /locus_tag="PK281_10320"
     CDS             complement(368..766)
                     /locus_tag="PK281_10320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49779.1"
                     /translation="MVEYNNHFEIHSEDRGSYTYIHASGRVKTREELLQFADKLFSEI
                     TRYNHTLIYNDMLGMDFLAKNSDYLALVESYENDDPQLVFEWKIAISIRPEFETVGSF
                     WESACYNRGFNYKSYTSEKKALNWLLEKFE"
     gene            complement(919..1317)
                     /locus_tag="PK281_10325"
     CDS             complement(919..1317)
                     /locus_tag="PK281_10325"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HOY49780.1"
                     /translation="MKTGSIEQTVVFDAEPQAVYDMLMDDQKHAAFTGSEVEMSSDVG
                     GKFVAFGGYVHGSNVELQPGKRIVQKWHFEEDGWPEDHYSTCIFEFEREGDKTILHFT
                     QTDIPANKVEALTSGWYEYYWEPIKAELVH"
     gene            1681..3462
                     /locus_tag="PK281_10330"
     CDS             1681..3462
                     /locus_tag="PK281_10330"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOY49781.1"
                     /translation="MEPTNIKDPEYFHKVVDCQYACPAHTPVPEYIRLIAQEKYDEAY
                     MVNWDSNVFPGVLGRTCDRPCEPACRRGRVEEEPVAICRLKRVAADNKGNVKNLMPQG
                     PFKPNGKKIALIGAGPASLTVSRDLAPLGYEIHLYDEQKLGGGFMRSQIPSFRLPETV
                     LNEEVNYILDLGIHTHFNHYVSSLKELLAKDYDAVFIGSGAPKGRDLPELPGRSEGDS
                     NIHIGINWLASVAFEHTNKIGKKVIVLGGGNTAMDCCRTARRLGGEEVKVVVRSPFAD
                     MKASPWEKEDAQHEDIPIIDNHVPKSFVVEHGVLKGMMFSKVHPVFENGKRKLVPTGE
                     ADVFIEADDVLVAIGQENSFPWLERDLGVEFDEWNMPIVNETTFVSTNPKVFFGGDAA
                     WGPKNVITAVAHGHQAAISIDLFCRNESLTNRPSPFVNLVSQKMGLHEWAYESEVVTD
                     VRYIVPQADKKLTLPDRKKEVELGFSKLDGYKEAQRCLNCDVQTVFTETKCIECDACM
                     DICPTSCITFVENDDETDLRASLKMPANNLSQDIYVSEPLKTTRVMVKDENVCLHCGL
                     CAERCPTSAWDMQKFFFNVTKAHTGCL"
     gene            3453..>3865
                     /locus_tag="PK281_10335"
     CDS             3453..>3865
                     /locus_tag="PK281_10335"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOY49782.1"
                     /translation="MSVNHIINDFVIRFANVNGTGSASANYLFCKAIFRMGIPVTPKN
                     IFPSNIQGLPTWYEVRVSEKGYLGRREGIDFMVSVNPQSMADDVANVRSGGYFLYDST
                     KQLHPRFIREDVNYIGIPLMEMCNNAFTDARQRQL"
BASE COUNT         1131 a          786 c          714 g         1234 t
ORIGIN      
        1 gaagaatcaa tatccgattc ttccccaata acaatttcat taattaaata ccttatttct
       61 gcatctcctt ttggaaacca aggtatgcta gtacaggtga gctcttgttg taatgtttta
      121 agtaaggaca tgaaatccca aaccgcataa acatgaaatt gcatgaacaa ctttacatca
      181 tccattcttt tcatttgctt aaacacggaa tggtgaatat tctctagtcg attcgactca
      241 attgctaatt ttaattgttc agaatagtcc attgggcttt ttcattcaga ttccagccgc
      301 aaaaataagc ttagtaataa cgatatgctt aggtgttaat ctctatattt tggatattta
      361 attttaatta ttcaaacttt tctagtaacc aattcaaagc ctttttttct gaagtatatg
      421 atttataatt gaatccacga ttgtaacatg ccgactccca aaaacttcca acagtttcaa
      481 attcaggcct aatactaata gcaattttcc attcaaatac aagttgagga tcatcatttt
      541 cataactctc aaccaaagct aagtaatcac tattcttggc cagaaaatcc atgcccagca
      601 tatcattgta aatcaatgtg tgattatagc gtgtaatctc tgaaaataat ttatctgcaa
      661 actgtaaaag ctcctcacgt gtttttacac gaccacttgc atgaatatat gtgtaactac
      721 ctcggtcttc tgagtgaatc tcaaaatggt tattatattc aaccataagc atattgattt
      781 ttaggattta caattataca cctaagccat ttcatagcag gtttttaaat cttttcgata
      841 tacttgagaa tcaggataat taccaaaatg aaagttaggt tgtgccttct tgtatcaaac
      901 taaacatgtg caataatttt aatgcactag ttcagccttt attggctccc aataatattc
      961 ataccatcca cttgttaagg cttctacctt atttgctggt atgtcggttt gtgtaaaatg
     1021 caatattgtt ttatcccctt ccctttcaaa ttcaaaaata caagttgaat aatgatcttc
     1081 tggccaacca tcttcttcga aatgccattt ttggacaata cgcttacctg gctgtaattc
     1141 tacattacta ccatgcacat atccaccgaa agcaacgaat tttccaccca catctgaact
     1201 catctcgact tctgatccag taaatgctgc gtgtttttga tcatccatca gcatgtcata
     1261 aactgcttgt ggttctgcat caaatacaac tgtttgttca attgatcccg ttttcatttt
     1321 gttgtgaatt tgaaatccta agttaaagat atcttacctt aaaaaacaag tcatcctagt
     1381 tctcttattg ctttccaaca cgactctttc agcacttccg aatttagaat ttctgaccat
     1441 agtttagtag aaaacaatcg ttagcaatga ttcttttatt cattcatttc ataaaacacc
     1501 ttctatttca ttgcttttca agggaatcga ttttttagct attatctctt tcttcttgaa
     1561 tgttggttat taaaaaactc ttattattac gaactagtta ctgattcatt tttgattcta
     1621 tcctcaaaca tagaagccta atttcagtat ctaaatgatg ctatcaatcc taacgtatac
     1681 atggagccaa ctaacattaa ggaccccgaa tactttcata aagtagttga ttgtcaatac
     1741 gcttgtcctg cccacactcc cgttcccgaa tatattcgcc tgattgctca ggagaaatac
     1801 gatgaagctt atatggttaa ctgggattcc aatgtgtttc ctggtgtttt aggtcgtacc
     1861 tgtgaccgcc cttgtgagcc tgcctgtcgt cgtggtcggg tagaagagga accagttgct
     1921 atttgccgcc tcaagcgtgt ggcagccgat aacaaaggca acgtgaaaaa tcttatgcct
     1981 caaggtcctt tcaaacctaa tggaaagaaa atagctttga ttggcgctgg accagcttct
     2041 cttactgtta gtcgtgacct tgctcctctt ggctatgaaa tccatctcta cgatgaacaa
     2101 aaactcggtg gtggtttcat gcgtagccaa attccatcat tccgccttcc ggaaactgta
     2161 ctaaacgaag aagtaaacta cattctagat ctcggaattc atacgcattt caatcattat
     2221 gtttctagct tgaaagagct tttggcaaag gattatgatg cagtttttat cggttcaggt
     2281 gcacccaaag gtcgcgatct gcctgaatta ccaggtcgtt cagaaggaga ttcaaatatt
     2341 cacattggta taaactggtt ggctagtgtt gcttttgaac acaccaataa aatcgggaag
     2401 aaagtcatcg ttttaggtgg tggaaatacg gccatggact gctgtcgaac cgctcgtcgt
     2461 cttggtggtg aagaagtgaa agtggttgtt cgtagccctt ttgctgatat gaaagcctca
     2521 ccttgggaga aagaagatgc acagcacgaa gacattccta tcatcgataa tcatgttcct
     2581 aaaagtttcg tggtggaaca tggtgttctt aaaggaatga tgttcagcaa agtacatcct
     2641 gttttcgaaa acggaaaacg taaactcgtt ccaacgggcg aagcagatgt attcattgaa
     2701 gctgatgatg tactcgttgc tatcggacaa gaaaatagtt tcccatggct agaacgtgat
     2761 ttaggtgttg aattcgacga atggaatatg ccaatcgtaa atgaaacaac attcgtttct
     2821 actaatccaa aagtgttctt tggtggcgat gctgcttggg gacccaaaaa tgtcattaca
     2881 gcagttgctc acggacatca ggctgctatc tctatcgatc ttttctgtcg gaacgaatca
     2941 ttaaccaatc gtccttcacc attcgtaaat ctagtaagtc aaaagatggg acttcatgag
     3001 tgggcgtatg aaagcgaagt ggtaactgat gttcgttaca ttgttcctca agcggataag
     3061 aagctgactc ttccagatcg aaaaaaggaa gttgaacttg gatttagcaa acttgatggt
     3121 tacaaagaag ctcaacgctg cttgaactgc gatgtacaaa ccgttttcac cgaaaccaaa
     3181 tgcatcgaat gtgacgcctg catggatatt tgtccaacat cctgcatcac cttcgtcgaa
     3241 aatgatgatg aaaccgattt gcgcgctagc ttgaaaatgc cggccaataa tttgagtcaa
     3301 gatatttatg tgtccgaacc gcttaaaacg actcgtgtca tggtgaagga cgaaaatgtg
     3361 tgtctgcatt gtggactatg tgccgaacgt tgtccaacct cagcttggga catgcagaaa
     3421 ttctttttta acgttacaaa agctcataca ggatgtctgt aaatcatatc atcaacgact
     3481 ttgtcattcg ttttgccaat gtcaatggaa caggttcggc cagtgccaat tatttgttct
     3541 gtaaagccat tttccggatg ggaattccgg ttacacctaa aaatattttc ccttccaata
     3601 ttcagggatt acctacttgg tatgaagttc gggtgagcga aaaaggctat ctcggtcgtc
     3661 gcgaaggcat cgattttatg gtgtcggtta atccgcaaag tatggccgat gatgtggcaa
     3721 atgtgcgttc tggcggatat ttcctttacg atagtaccaa gcaactacat cctcgtttta
     3781 tccgtgaaga cgttaattat attggcattc cgctcatgga aatgtgcaat aatgcattca
     3841 ctgatgcacg tcagcgacaa ttatt
//
LOCUS       DAOOQW010000224         3849 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_28226_length_3849_cov_4.059304, whole genome shotgun sequence.
ACCESSION   DAOOQW010000224 DAOOQW010000000
VERSION     DAOOQW010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3849
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28226_length_3849_cov_4.059304"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2352)
                     /locus_tag="PK281_10340"
     CDS             complement(<1..2352)
                     /locus_tag="PK281_10340"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOY49783.1"
                     /translation="MQNRFSTYNPVKGLIAGSWILFVLCLISACNPTRRLQKEEYLLN
                     RNIIINNKTIITDAELLTYVRQKPNRKIVGLWRFHLWIYNTVNQEKFKPRYEARIEKR
                     KLLNEKRKAAGKKLKDPEPLSLAKWRLTVGEAPVILDTALLNRSSKQIELLLKNHGYF
                     NAIVKDSVAFRKNRKFWKNRKNMKNAFFTIEAGTPYTIKSITYDIEDPSIRDLVMSDK
                     KNSLIATGKNFNTDDLDLERVRITEALKNKGYFAFVKNFINYKADSSLNSHQVDITLE
                     IENPVKRVAGYVDSTISYSHVRYKINNIYVLADYSLRRDSTESPDTLFFDNIHYITEG
                     KLKFRPRAIKPAITFNKGDLYSKIEADKTYRRISDFKTFKFINIQFKPSAGGDSTGLL
                     DCEIQVSPYLRNAYTIQTQGTNKAGDLGVSGDFIYQNRNFLRGLEQIEFRLNTTLEVQ
                     RIISDVNDENQTIQEFLPFNTILFGPVVSLKLPKIPRFMNFLGKSGQKTNITTSYNFQ
                     QRPDYKRNIFNLAYGFTAKSGTRVTNTFNPAEINFVNVNLGTEFSALLDKSNNLFLKN
                     SFQSQLISSMRYSFIYNSQKVGESKNFLFFQGNLENSGLVLSATRPLFQSPAKNDEGK
                     YIVFGVPFSQYLRFDTDLRYYKFMGRSSSIAMRSIVGLGIPYGNSKVMPFVKSFFGGG
                     ANGNRAWIARSLGPGGYQNPTGIRFDQIGDIKLEWNLEYRSKIYKIFEGAAFVDAGNI
                     WLRKVDTQRPLADFDLSRFYKEIAIGVGGGLRLNFDYFIIRLDV"
     gene            2399..3181
                     /locus_tag="PK281_10345"
     CDS             2399..3181
                     /locus_tag="PK281_10345"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOY49784.1"
                     /translation="MLGKNRLRELQQLHQKKFRESQGKFLAEGAKIVPELLRSNFPVE
                     EIFARDFWLSKNHALLNESKCKVTEIAQNDLERLSQLQTPQEVIALCAKPVDKLPGIQ
                     AGKLMLILDSIRDPGNMGTLIRLADWFGLDGVLATEDSVEWTNPKVIQATMGSFIRVR
                     PYYMDVASLISPTSFRLPVYAADLDGTNLYSGDYNASGFIIISNEAHGLSPELEHFVS
                     EKIHIPAFFKQGSGPESLNAAMAGAIVLGEFSRRRMKKAENG"
     gene            3193..>3849
                     /locus_tag="PK281_10350"
     CDS             3193..>3849
                     /locus_tag="PK281_10350"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HOY49785.1"
                     /translation="MYKAFIRPFLFLFSPEKAHAISVRMFKILLYVPGFEGIVRWYSN
                     GFDRRLRLDKKGLRFPNRVGLAAGFDKNATFFQDFRHLGFGFIEVGTVTPLAQDGNPQ
                     PRLFRLTEDEAIINRMGFNNLGVEAMTERLKKRPAGLIIGGNIGKNKLTPNEQAVSDY
                     LICFRELQPYVDYFAVNVSSPNTPGLRALQEKEPLKALLHALQEEARHMGADQPVLLK
                     I"
BASE COUNT         1130 a          726 c          753 g         1240 t
ORIGIN      
        1 aacgtccaat cggatgatga aataatcaaa gtttaatcga agtccaccac ctacaccaat
       61 ggcgatttct ttgtaaaagc gacttaaatc gaaatcagca agtggtcgtt gcgtatctac
      121 ttttcggagc caaatattac ctgcatcaac aaaagcagca ccttcgaaaa tcttataaat
      181 cttcgatcga tattcaaggt tccattccaa tttgatatct ccaatttggt cgaatcgaat
      241 tccagttgga ttttgataac cacctggacc aagggaacgt gcaatccaag ctcgatttcc
      301 attggcgcca ccaccgaaga aacttttcac gaaaggcatt actttactgt ttccatatgg
      361 aattcctaaa ccaacaatac tgcgcatggc aatagaagaa gatctaccca tgaatttgta
      421 ataacgcaaa tcggtatcga atcgtaaata ctgcgaaaat ggcaccccga atacgatgta
      481 ttttccttca tcgttttttg caggagattg gaatagtggg cgagttgcac ttaaaactaa
      541 accggagttt tcgaggttgc cctgaaagaa caagaagttt tttgattcac ctactttttg
      601 agaattgtag atgaaactat atcgcatgga agaaattagc tgactttgaa aagagttctt
      661 cagaaataga ttgttcgatt tgtcgagcaa tgcagagaac tctgttccca aatttacatt
      721 cacgaagttg atttcagctg ggttgaatgt attggtaact cgtgtccccg atttagctgt
      781 gaatccatat gctaagttga agatattcct cttgtaatcg ggcctttgct ggaaattgta
      841 ggatgtagtg atattcgttt tttgtccact tttccctaag aaattcatga agcgtggaat
      901 cttaggaagt ttcaaggata ctaccggtcc aaataatatg gtgttgaaag gtagaaattc
      961 ttggatggtt tggttttcgt cgtttacatc tgatataatt cgctgaacct ctaaggtcgt
     1021 attcaaccta aactcaattt gttccaatcc acgcaggaag tttcggtttt gataaataaa
     1081 atcccccgaa acccctaaat cgccagcctt atttgtccct tgagtttgta tcgtataagc
     1141 attccgcaag taaggactaa cctgtatttc gcaatcgagt aatccggtag aatcgcctcc
     1201 agcactgggt ttaaactgaa tgttgatgaa tttgaacgtt ttaaaatcgg agattctacg
     1261 ataagtttta tccgcttcaa ttttgctgta caaatctcct ttattgaatg ttatagctgg
     1321 tttaatagca cgaggacgaa attttaattt gccttcggtg atataatgga tattatcaaa
     1381 gaataaggta tcaggcgatt cggtactgtc tcttctcagg ctataatccg ctagaacata
     1441 tatgttattt atcttgtaac gaacatgcga ataactaata gtactatcca cataacctgc
     1501 aacccgctta accggatttt caatttctaa ggtaatatca acttgatgtg aattgagtga
     1561 actatcagct ttgtaattga tgaagttttt tacaaaggca aaatatccct tatttttaag
     1621 tgcttcggta attctaacac gttctagatc tagatcatca gtattgaaat tttttccagt
     1681 cgcaatcaag ctatttttct tgtcgctcat gactaaatca cgaatacttg gatcttcaat
     1741 atcataggtt atggatttga tcgtataagg agttccagct tcgatagtga aaaacgcatt
     1801 tttcatattc ttcctattct tccaaaactt cctatttttt cgaaatgcga cagaatcctt
     1861 tacaatagcg ttaaaataac cgtggttctt cagtaaaagt tcaatttgtt tggaagaccg
     1921 attgagtagt gctgtgtcga gaattacagg agcttcgcca actgttaaac gccatttggc
     1981 taaactcaaa ggttctggat cttttaattt tttgccagct gcctttcgtt tttcattcaa
     2041 tagtttccgt ttttctattc tagcctcata acgaggtttg aatttctcct gattcactgt
     2101 attgtaaatc cacaaatgga atcgccaaag tcctacaatt tttcgattgg gtttttgacg
     2161 cacataggtc aataattcag catcggtaat gattgtttta ttattgataa taatgtttcg
     2221 attgagcaga tattcctctt tttgaaggcg tcgcgtagga ttacaagcag aaattaaaca
     2281 caacacgaat agaatccatg atccggcaat caatcccttt actgggttat acgtggaaaa
     2341 ccggttttgc atccgctctt tatggtactt tagctgcttc aaaaatacgt gcttttacat
     2401 gttgggcaaa aatcgtcttc gtgaactgca acagttgcat caaaagaaat tccgcgaaag
     2461 tcaaggtaaa tttctagccg aaggagcgaa aatcgttcct gaattattgc gttcgaactt
     2521 tcctgttgaa gagatttttg cacgtgattt ttggcttagc aagaaccatg cgctcttgaa
     2581 tgaatccaag tgcaaagtaa ctgaaatcgc tcagaatgat ctcgaacgtt tgagtcagct
     2641 tcaaactcct caagaagtaa ttgcattatg tgctaagccg gttgacaaac tacctggaat
     2701 tcaagcgggt aagttaatgt tgattttaga ttccattcgt gatcccggta atatgggcac
     2761 cttaattcga ttagctgatt ggtttggttt agatggagtt ttagctacag aagacagcgt
     2821 tgaatggacc aatcctaaag tgattcaagc tacaatgggt tcatttatac gtgtgcgacc
     2881 atattatatg gatgtagctt cgcttatatc tcccacatcg tttcgattac ctgtttatgc
     2941 agccgatttg gatggaacga atttatattc gggcgattat aatgcaagtg gattcattat
     3001 tattagcaat gaagcgcatg gattaagtcc tgaacttgaa catttcgtct cagaaaagat
     3061 tcatattccg gcatttttta aacaaggttc aggtcctgaa tcgcttaatg ctgcaatggc
     3121 tggagcaatt gttcttggtg aattcagtag aagaaggatg aaaaaagcgg aaaacggtta
     3181 attttaccca caatgtataa agcatttatc cgcccatttc ttttcctttt ttctccagaa
     3241 aaagcacatg ccatttcggt tagaatgttt aaaattctgc tttatgtacc tggatttgaa
     3301 gggattgttc gttggtattc gaatggcttc gatcgtcgtt taaggttgga taaaaaagga
     3361 cttcgctttc ccaatcgagt aggattagca gcgggttttg acaagaatgc taccttcttt
     3421 caggattttc gtcatctcgg ttttggtttc atcgaagttg gaaccgtaac acctcttgcc
     3481 caagatggaa atccacaacc tcgacttttt cgtttaacag aagacgaagc tataataaac
     3541 cgtatgggct ttaataattt gggtgttgaa gccatgaccg agcgattgaa aaaacgtcca
     3601 gctggactta tcattggagg aaatattggc aagaataagt taacacctaa tgaacaagca
     3661 gtaagcgatt atctcatctg ctttcgtgaa ttacagcctt atgttgatta ttttgccgta
     3721 aatgttagct cacctaacac tccgggtttg cgcgccttac aagagaaaga gccgttaaaa
     3781 gctttgctgc atgctttgca agaagaagca cgacacatgg gagctgatca acctgtatta
     3841 ttaaaaatt
//
LOCUS       DAOOQW010000225         3847 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_28246_length_3847_cov_3.963344, whole genome shotgun sequence.
ACCESSION   DAOOQW010000225 DAOOQW010000000
VERSION     DAOOQW010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3847
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28246_length_3847_cov_3.963344"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..712)
                     /locus_tag="PK281_10355"
     CDS             complement(<1..712)
                     /locus_tag="PK281_10355"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOY49786.1"
                     /translation="MAHNNSLLSGIQQVGIGVTDAEAAFTWYRRNLGMDVPVFKDAST
                     AELMVRYTGNEARKRYAILAMNMQGGGGFEIWQYTSRVPQPAAFQIQAGDLGIFGILM
                     KCRDVKAAHASYKAKGLAVSDVFPTPDGGSRFHLSDPYGNRFTFTPGLDWFDTGKHPN
                     GGITGVQIGVSNMDHSLKFYQETLGFNQVVFDQIGPAPELGSGHFRRVLLRPNPAFGG
                     AFRRLLGAAEVELIQSMQH"
     gene            complement(717..1517)
                     /locus_tag="PK281_10360"
     CDS             complement(717..1517)
                     /locus_tag="PK281_10360"
                     /inference="COORDINATES: protein motif:HMM:NF004825.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOY49787.1"
                     /translation="MENKTALKVYWITGASSGIGEALALQLAKPNNALILSGRNEQAL
                     EKVRQACSSHTTHVAVLPFDMLDLSAFPRLIDKAISFFGHVDVLVNNAGTSQRSLAIE
                     TSEAVERKLMELDYFAPVALSKALLPHFISRKSGHLVVVSSIAGKFGFALRSTYAAAK
                     HALLGYFEALRLELKDIPIYVTVICPGRVKTNISFSAVKSDGSPHGVMDDGQRNGVSA
                     EKCARAIDKAIQRKTPEILVGKAELIPVFLRRYCPPLFYFLIKRVKAT"
     gene            1620..3212
                     /locus_tag="PK281_10365"
     CDS             1620..3212
                     /locus_tag="PK281_10365"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HOY49788.1"
                     /translation="MMTVRILLVSLLLSTNIAAQNRIQISPKPVSVKENPGEFKLSSE
                     TEIVLWGGSLKQEAVYLRDYLNQYFQLNLKIDSSGKSKASSRIVLNYDRLDHPFPGAY
                     NMTVKSDEVYIGGDNPSGVFYGIQSIIQLLPSKAGEIPVIPNCEIQDSPRFAYRGVHL
                     DVSRHFFPVEYIKRYLDYIAYHKMNVFHWHLTDDQGWRIEIKKYPLLTEVGAWRDGTL
                     IGKHPGKGYDTIRYGGFYTQEQIKEVVAYATSRHVEVIPEIEMPGHSMAAIAAYPFLS
                     TTPDKPKKVAKGWVNYGDYEYNNVLNPSDTTFKFLEDVLTEVMELFPSQYIHIGGDEC
                     SKRWWKESTFCQKLKKEKGLKDEKALQSYFIQRVEKFVNSRGKSIIGWNEILQGGLAP
                     NATVMSWMGTSGGITAAKKKHYVVMTPGSACYFDHPQRANDDSTGLGKPVLIDSVYAY
                     NPMPKVLTPEFQKYILGAQANVWTEYIGNTSKLEYMLFPRLAALSEVVWTPQKDRSFS
                     EFKKRLVKQKERYEMWGINYSK"
     gene            complement(3376..3792)
                     /locus_tag="PK281_10370"
     CDS             complement(3376..3792)
                     /locus_tag="PK281_10370"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019670491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HOY49789.1"
                     /translation="MKILILCTGNSCRSQMAEGYLRHFLGNSAQVFSAGLEAHGLNPR
                     AVQTMKEDQIDISSHTSDILDKYLNTEFDYILTVCDNANENCPYFPGKAHRIHHSFPD
                     PAKASGTEEEVMNAFRSVRNQIKEWTRELAVQFIQK"
BASE COUNT         1188 a          740 c          820 g         1099 t
ORIGIN      
        1 tgtgttgcat cgattggatt aattctactt ctgcagctcc taataaacga cggaacgcac
       61 ctccaaaagc aggattaggt cgcaataaaa cacgacgaaa atgtccgcta cctagttcgg
      121 gtgctggtcc gatttgatcg aaaactactt gattaaatcc aagcgtttct tgataaaatt
      181 ttagtgagtg atccatattt gaaacaccaa tctgaacacc ggtaattcct ccattgggat
      241 gctttcctgt gtcgaaccaa tcgagtccgg gtgtgaaggt aaaacgattg ccatacggat
      301 cgcttaagtg aaaacgagag ccgccatcgg gcgttggaaa aacgtccgaa actgctaatc
      361 cttttgcttt gtatgaagcg tgtgcggcct tcacatcgcg acatttcata aggatgccga
      421 aaattcctaa atcgcccgcc tgaatttgaa atgccgcagg ttgaggaaca cgacttgtgt
      481 attgccagat ttcgaatcca cctccgcctt gcatattcat ggcgagaatt gcatagcgtt
      541 tacgcgcttc atttccagta taacgtacca ttaattcggc agtagaagca tccttaaaaa
      601 caggaacatc catgcctaaa ttacggcgat accaagtaaa tgcagcttct gcatctgtaa
      661 ctccaattcc tacctgttgg ataccgctta atagcgaatt gttgtgtgcc ataaaatcag
      721 gtcgctttaa cgcgtttgat caaaaaataa aataaaggtg gacaatacct ccttaaaaaa
      781 accggaatca attcggcttt accaaccaaa atttcgggtg ttttacgctg tatagcttta
      841 tcgattgcac gagcgcattt ctcagcactt acaccatttc gctgaccatc atccataact
      901 ccatgaggcg agccatcgga ttttactgcc gaaaaagaaa tattggtttt gactcttccc
      961 ggacaaataa ctgtcacata aatcggaata tctttcaatt cgagacgcaa tgcttcgaag
     1021 tatcctaaca atgcgtgttt tgcagcagca taggtacttc taagtgcgaa accaaatttc
     1081 ccagcaatgc tagaaaccac aactaggtgt cctgattttc gtgaaataaa atgaggcaac
     1141 aatgctttgg aaagtgcaac aggagcaaaa taatcgagtt ccatgagctt tcgttccact
     1201 gcttcggatg tttcgatagc aagcgaacgt tgtgatgttc cggcattatt gaccaacaca
     1261 tctacatgtc cgaaaaatga aatggcttta tctatcaatc taggaaatgc agataaatcg
     1321 agcatatcga aaggtaaaac ggcaacatga gtagtatgtg aagaacaagc ctgacgtact
     1381 ttttctaagg cttgctcgtt tctaccactc agaatcagag cattattggg tttagcgagt
     1441 tgcaatgcca aagcttctcc aatacctgaa gaggcgcccg taatccaata tactttgaga
     1501 gctgttttgt tttccacgta ggggacgaaa ttagagaatt gccgcaactc acgaaaggat
     1561 gttgaatcat tcctgtcaat tatccagtta caggcttgtt taagctttaa ttttatcgca
     1621 tgatgactgt tcgaatttta cttgtaagtc tgctcctgtc gactaatatc gctgctcaaa
     1681 accgaattca aatttcaccc aaaccagtat cggtaaagga aaatccaggt gaattcaagc
     1741 ttagttcaga aacagaaatt gtcctgtggg gtggatcttt aaagcaggaa gcggtttatt
     1801 tacgtgatta cctcaaccag tattttcagt tgaatcttaa aatcgattct tcgggaaaat
     1861 ctaaagctag ttcgcgtatt gtgcttaact atgatcgatt agatcatcct tttccgggtg
     1921 cctataacat gactgttaaa agtgatgaag tatatattgg cggagataat ccgagtggag
     1981 ttttttatgg cattcaaagt atcattcagc ttttaccaag caaagcaggt gagattccag
     2041 taattcctaa ttgtgaaatt caggattctc ctcgatttgc atacagaggt gttcacttag
     2101 atgtaagccg acattttttc ccagttgaat acatcaaacg ctatctggat tacatcgcct
     2161 atcacaaaat gaatgtattt cattggcatt tgaccgatga tcaaggatgg agaatagaaa
     2221 taaagaaata tccattatta actgaagttg gagcttggcg tgatggtaca ctcataggta
     2281 aacatccagg taaaggctat gataccattc gttatggtgg attttacacg caagaacaaa
     2341 tcaaggaagt ggttgcttat gctacatcgc gacatgtaga agtgattccc gaaatagaaa
     2401 tgcccggaca tagtatggca gccattgcag cctatccatt tttaagcaca actcccgata
     2461 aacctaagaa agtagcaaaa ggctgggtaa attatggtga ttacgaatac aacaatgtgt
     2521 taaatccgag cgataccacc ttcaaatttt tagaagatgt gctgacagaa gtaatggaac
     2581 tatttccttc gcaatacatt catattggtg gtgatgaatg ttctaaacgc tggtggaagg
     2641 aatcaacgtt ttgtcagaaa ttgaagaaag aaaaggggtt gaaggatgaa aaagcgcttc
     2701 agagttattt tatacagcgg gttgagaaat tcgtgaattc gagaggcaag tcaatcattg
     2761 gctggaacga aattttacaa ggaggattag ctcccaatgc aactgttatg agttggatgg
     2821 gaacatcggg tggaataact gctgcaaaga aaaaacacta cgtcgtaatg actccgggaa
     2881 gtgcatgtta ctttgatcat ccacaacgag ctaatgatga ttccactggt ttaggaaaac
     2941 ccgttttgat agatagcgtt tatgcctata acccaatgcc taaagtgcta acaccggaat
     3001 ttcaaaagta tattttagga gcacaagcaa atgtttggac cgaatatatt ggtaatactt
     3061 ctaaactaga atacatgctt tttcctcgat tagctgcatt gagtgaagtc gtatggacac
     3121 cccaaaaaga tcgcagtttt tccgaattca aaaaacgctt ggttaagcaa aaggagcgat
     3181 atgagatgtg ggggattaat tacagtaaat gatgtgctgg tttgctgatg tgctagttaa
     3241 tgtgatggtg tgatggtgtg ctcccttcga caggctcagg gagcggagtt tctaacccac
     3301 aaagcccgcg cgctgagctt gtcgaagcga gtggttgatt atggaatcaa aaaaattaaa
     3361 ggataatagt ccaatttatt tttggatgaa ctgaactgct aattcgcgcg tccattcttt
     3421 aatttgattg cgaacggatc tgaacgcatt catcacttct tcttcggtgc cggaagcttt
     3481 ggcgggatcg gggaaactat gatgaatgcg atgagctttg cctgggaaat atggacaatt
     3541 ctcattcgca ttatcgcata cagttagaat ataatcaaat tcagtattga ggtatttatc
     3601 taggatatcg gaagtatgtg atgatatatc gatttgatcc tctttcatag tttgaactgc
     3661 tcgaggattt aaaccatgtg cttcgagtcc agctgaaaaa acttgagcag aatttcctaa
     3721 aaaatgacgc aaataacctt cagccatttg agatcgacaa gaatttccag tgcagaggat
     3781 gagaattttc atttaattac ggattatcaa ttacgaatta cgaatgggga attacgaatt
     3841 atcaatt
//
LOCUS       DAOOQW010000226         3846 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_28258_length_3846_cov_4.672910, whole genome shotgun sequence.
ACCESSION   DAOOQW010000226 DAOOQW010000000
VERSION     DAOOQW010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3846
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28258_length_3846_cov_4.672910"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..796
                     /locus_tag="PK281_10375"
     CDS             <1..796
                     /locus_tag="PK281_10375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CofH family radical SAM protein"
                     /protein_id="HOY49790.1"
                     /translation="IVGGVHPKMGLQYFASLIKKIKEIRPDLHVKAFTAVELDYMCRK
                     AKMSYAEGLQVLKDHGQDSLPGGGAEIFDEKLRAEICGDKCTATEWLEIHETAHKLGM
                     PSNATMLYGHIETFAHRIDHMERLRQLQDRTGGFNTFIPLKFRNKDNQMSAVDEVSVI
                     EDLRNYAISRIYMDNFGHIKAYWPMIGRNTAQLSLSFGVDDMDGTIDDSTKIYSMAGS
                     EEQNPSLTTPQLVEMISTAGKIPVERDTLYQVIRDYSQEPLTLEEV"
     gene            803..1306
                     /locus_tag="PK281_10380"
     CDS             803..1306
                     /locus_tag="PK281_10380"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HOY49791.1"
                     /translation="MIQSNINNQSFQVEFEKDGKTGTVNGSSFTWDLLPITTNRFNIL
                     RNNRSYTIIIESREPETNSLVLRINGKKLSVQTKDKMALLLESMGLSAISALKVNEVK
                     APMPGLVLRALVEAGDQVKKGDSLLVLEAMKMENVIKSPVDAEIAKIVVKQGQAVEKN
                     QVLIQFV"
     gene            complement(1307..2338)
                     /locus_tag="PK281_10385"
     CDS             complement(1307..2338)
                     /locus_tag="PK281_10385"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008991632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOY49792.1"
                     /translation="MTLTEEIERIKADINAIALQTVEETEQYRIKYLSRKGIVPALFE
                     RFKEVTLEERKTIGKLLNELKQKAELTWKESLEKVQDNQDTNSAIGFDPSAPGRPFQR
                     GSRHPLSVVANEINSIFSRLGFTIAEGPEIEDDWHIFSALNFPEEHPARDMQDTFFIE
                     KNPDVALRTHTSSVQIRMMESKQPPLRMLMPGRVYRNEAISARAHCFFHQVEGLYVDE
                     NVSFADLKQTLLTFSREMFGEETRIRLRPSYFPFTEPSAEMDISCTVCKGAGCNICKY
                     TGWVEILGCGMVHPNVLENCGIDSKKYTGFAFGMGIERIAILKYRVKDLRLFSQNDMR
                     FLKQFETEW"
     gene            2473..2835
                     /locus_tag="PK281_10390"
     CDS             2473..2835
                     /locus_tag="PK281_10390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49793.1"
                     /translation="MKLLSRFTLILMTLVALSTGVSCGKKDTTCTAVIKVIRTNGSAV
                     SGAKVRLTSNAGLATSKELADYLPADELTDANGLATYEFKYPGILDVEVTHIAYGSGT
                     DLIKLEVGETVNKTVTIQ"
     gene            complement(2822..3208)
                     /locus_tag="PK281_10395"
     CDS             complement(2822..3208)
                     /locus_tag="PK281_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014032488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49794.1"
                     /translation="MSSYKQIRHTESHLQELEANWKALLAELNSQFDGDLDLQAVLFL
                     IGVQELGKGVRKYTKDQKLELMHIAICTLLEPYGYYNFSGNDEDGFPHWELNEQLPPL
                     SPGQQLALMKECVLDYFSARKELTEL"
     gene            complement(3208..>3846)
                     /locus_tag="PK281_10400"
     CDS             complement(3208..>3846)
                     /locus_tag="PK281_10400"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY49795.1"
                     /translation="SLVSPVIDFSSVTSPTNISFKVAYARRSASTNDKLEVWISSNCG
                     QTWTRRYAKLGATLATTSSLITSSFTPSSTQWRTETVSVTPLAGEEHGMVKFTVIDSS
                     GNNLYLDDINLVNTPAGITEILDEYSTQIFPNPGSGDAQVNIGLFTPEEVSVSILDVT
                     GRLLGTKNLGMKSSGEHTIWLRDISGSYLASGAYFIRIEAGNQTMIRKWIGN"
BASE COUNT         1191 a          772 c          727 g         1156 t
ORIGIN      
        1 tattgtaggc ggagttcacc ccaaaatggg cttgcagtac tttgctagct taatcaagaa
       61 aatcaaagaa attcgccccg atttgcatgt gaaagctttt actgctgtcg aactcgatta
      121 catgtgccgg aaagcaaaaa tgagttatgc cgaaggactt caagttttga aagatcacgg
      181 acaagattct ttaccaggtg gcggagccga aatttttgac gaaaagctac gtgccgaaat
      241 ttgtggtgat aaatgcacag ctaccgaatg gctcgaaatt cacgaaacgg cccataaact
      301 tggaatgcct tccaatgcta ccatgttata cggccatatt gaaacgtttg cacatcgcat
      361 cgatcacatg gagcgattaa gacaattaca agatagaact ggaggtttca atacttttat
      421 tcctttgaaa tttaggaata aggataacca aatgagtgct gtagatgagg tttcggtaat
      481 tgaagattta agaaattacg ccatctctcg aatttacatg gataacttcg gtcatatcaa
      541 agcctattgg ccaatgatag gtcgaaacac cgctcaactt tctctttcat ttggtgtaga
      601 tgatatggat ggaacaatcg acgattccac caaaatctat tcaatggctg gttctgaaga
      661 gcaaaacccg agtttgacta ctcctcaact tgttgaaatg attagtacag ctggtaaaat
      721 tccagttgaa cgcgatactt tatatcaagt tattcgtgat tattctcaag aaccactaac
      781 actcgaagaa gtttgatttg ctatgattca atctaacatt aacaatcaat cctttcaggt
      841 agaattcgaa aaagatggga aaacaggaac cgtaaatggt tcttcgttta cttgggatct
      901 tcttccaatc acgaccaatc gctttaatat tctaagaaat aaccgttcct acacgattat
      961 tatcgaatca cgcgaaccag aaacgaactc gttggtattg cgcataaatg ggaagaaatt
     1021 gagcgttcaa actaaagata aaatggcgct acttctcgaa tctatgggct taagtgcaat
     1081 ttcagctttg aaagtaaatg aagtgaaagc ccctatgcca ggattggttc tgcgtgcatt
     1141 ggttgaagca ggtgatcaag tgaaaaaagg cgatagctta ctcgttctcg aagccatgaa
     1201 aatggagaac gtgattaaat cacctgttga tgccgagatt gctaagattg ttgtaaaaca
     1261 agggcaagca gttgaaaaga atcaagtttt gattcaattc gtatgattac cattcagttt
     1321 caaactgttt aagaaaacgc atatcattct gggagaacaa gcgcagatct tttactctgt
     1381 acttaagaat agcaatacgt tctattccca tcccaaaggc aaatcccgtg tatttcttgc
     1441 tgtcaatacc acagttttca agcacgttag gatgaaccat tccacagcct aggatttcaa
     1501 cccaacccgt gtatttgcaa atattgcaac cagctccttt gcaaacggta cacgaaatat
     1561 ccatttcagc tgaaggttca gtgaatggga agtaagaagg acgaagtcgg atgcgcgttt
     1621 cttctccaaa catttctctg gaaaaagtaa gtagcgtttg tttcaaatca gcaaacgaga
     1681 cattctcatc tacatataag ccttctactt ggtggaaaaa acaatgtgca cgtgctgata
     1741 ttgcctcatt ccgataaaca cgacctggca ttaacattct taatggtggc tgcttacttt
     1801 ccatcatacg aatttgtacc gatgaagtat gcgtacgtaa agccacatct ggattcttct
     1861 caataaagaa tgtatcctgc atatctcgcg caggatgttc ttctggaaaa ttgagcgcag
     1921 agaaaatgtg ccaatcatct tctatttctg gtccttcggc aatagtaaat cctaaacgag
     1981 agaatatgga attaatctca tttgcaacaa ccgataaagg gtgacgactt ccacgttgaa
     2041 atggacgacc cggagcactt gggtcaaaac cgatcgctga atttgtatct tgattgtctt
     2101 gaaccttttc taatgattct ttccaagtta actctgcttt ctgctttaat tcattcaaca
     2161 attttccaat tgtctttctt tcttctaacg taacttcctt aaaccgctca aataaagcag
     2221 gcacaattcc tttacggctt agatatttaa tgcggtattg ttccgtttct tcaacagttt
     2281 gtaaagctat agcgtttata tccgccttaa tacgctcgat ttcttcggtc aatgtcatgt
     2341 ttcactaatt cggcgctaag ataatcaacc tagtcccatc ttcgatgaaa caaagcaata
     2401 cgtaatgcgt ttactcattt tagaggtatc tctgaaagat ttttctattt ttgaacttct
     2461 caaaattcaa acatgaagct gttatcccgt tttactctca ttcttatgac tctagttgcc
     2521 ttatctacag gcgtttcctg tggtaaaaag gatacaacct gtaccgcagt cataaaagta
     2581 atccggacaa atggtagtgc cgtttcaggt gcaaaggttc gtttgacctc aaatgcaggt
     2641 ttagcaacaa gtaaagaatt agcagattat ttacctgctg atgaattaac cgatgcaaac
     2701 ggtttggcga catacgaatt taaataccca ggaattttag acgtagaagt tactcatatc
     2761 gcttatggaa gcggtactga tctaattaaa ctggaagttg gtgaaacagt aaataaaact
     2821 gttacaattc agtaagctct ttacgagctg agaagtaatc caatacacat tctttcatca
     2881 aggccaattg ttgccccgga cttaatgggg gcaattgttc gttgagttcc caatgcggga
     2941 agccatcttc atcattacct gaaaagttat aatacccata aggttcaagc aaggtgcaaa
     3001 tggcaatatg cattaattcc agcttctggt ctttcgtgta tttgcgcact ccctttccta
     3061 attcctgcac tccaattaag aaaagtacgg cttgaaggtc gaggtcacca tcaaattggg
     3121 aattaagctc cgccaataaa gccttccaat tggcttctaa ctcttgaaga tgagattcgg
     3181 tatggcggat ttgcttatag ctactcatta attaccaatc cattttctga tcatcgtttg
     3241 attgccagct tcaatacgta tgaagtatgc gccagatgcc aaatatgatc cactaatatc
     3301 gcgaagccaa attgtatgtt caccactaga tttcattcct aaattcttag tgcctaacaa
     3361 acgacctgtt acatcaagaa tagaaacgct tacttcttct ggtgtaaata aaccaatatt
     3421 tacctgagcg tcacccgatc ctggattagg aaaaatttga gtagaatatt catctaatat
     3481 ttctgtgatt ccagcaggtg tgttcaccaa attgatatcg tctaaataca aattattacc
     3541 actactatca attactgtga atttgaccat tccatgttct tctccagcta aaggtgtaac
     3601 acttactgtt tctgttctcc attgagttga cgatggagtg aatgaagatg ttattaatga
     3661 acttgtagtt gccaaggtag ctcctaattt cgcataacgg cgagtccaag tttgaccaca
     3721 attagaagaa atccaaactt ctagtttatc atttgttgaa gcacttctgc gtgcataagc
     3781 aactttaaat gaaatattgg ttggtgacgt aacagaagaa aaatcaatta caggagatac
     3841 taatga
//
LOCUS       DAOOQW010000227         3831 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_28414_length_3831_cov_4.581568, whole genome shotgun sequence.
ACCESSION   DAOOQW010000227 DAOOQW010000000
VERSION     DAOOQW010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3831
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28414_length_3831_cov_4.581568"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..773)
                     /locus_tag="PK281_10405"
     CDS             complement(<1..773)
                     /locus_tag="PK281_10405"
                     /inference="COORDINATES: protein motif:HMM:NF014045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HOY49796.1"
                     /translation="MLQPRDGVVRIGVFYDGNYFLHVSNYYNYFHQRRRRISIAGLHS
                     FIRQQIAEEMQVDYSLCHVVDAHYFRGRMSAYEASQRGNQLYNDRLFDDVLMSEGVVT
                     HYLPLRTRAGRKEEKGIDVWLALEALELSYMKKYDVLVLITSDGDFVPLIRKVNTIGT
                     RVMVLSWDFEYTDDTGREIITRTSQDLLEEVTYPVAMHEIIDSRASGNELIVGGLFVT
                     QESAPKTYENIIDKGDMKVSKTLNMKSGYGFVAYPPNNL"
     gene            complement(1164..2255)
                     /locus_tag="PK281_10410"
     CDS             complement(1164..2255)
                     /locus_tag="PK281_10410"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOY49797.1"
                     /translation="MNIRLYLLDKYIIKKFLGTFFYAVLLFGVLLPVIFDLTEKMDDI
                     LEKDIPIQELLIDYYLNFIPYFANLFTPIFGFISVVFFTSRMANQTEIVAILSSGVSF
                     KRFLRPFMISAIVVALFSFLLGSFIIPPANARRLKFEYKYLKYNWNRNFSNLHRQIAP
                     GTFVYLETYTQHSVTGFRFTQEGFKDQKMTYKMSADYIRWDSTSSSWKMDNLVIRKFI
                     GKKESIRKVALLDTTLLFTPADFAIADDDVEMMNFFELNRFIVQEQNKGSEFIDYYLL
                     ERQRRMANPISNIILALIAVPLAGRKVRGGVGLHIGIGIGISFSFVMLMQLSSVFATE
                     GGVNPALSAWIPNLIYFFVALGLIYKAPK"
     gene            complement(2252..3382)
                     /gene="tgt"
                     /locus_tag="PK281_10415"
     CDS             complement(2252..3382)
                     /gene="tgt"
                     /locus_tag="PK281_10415"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014083845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOY49798.1"
                     /translation="MHFTLAATDKHSKARAGEISTDHGKIPTPIFMPVGTAGTVKAVH
                     QRELRDDINARIILGNTYHLYLRPGLDVLRQAGGLHKFNGWDRPILTDSGGYQVFSLS
                     DNRKITEEGAMFKSHIDGSKHMFSPESVMDIQRTIGADIIMAFDECTPYPCTYDYAKH
                     SMDRTHRWLKRCIEHLDKQEPLYGYRQTLFPIVQGSTNRELRIQSAEFIAAQNRDGNA
                     IGGLSVGEPVEDMYEMTELVCSILPADKPRYLMGVGTPANILEGISQGVDMFDCVMPT
                     RNARNGMLFTSEGIINIRNEKWKTDFSAIDPLLITFADQQYTKAYLRHLFIGGEYLAG
                     MIASVHNLGFYLWLVEESRRRILDGSFATWKSEMVKKVSRRL"
     gene            3481..>3831
                     /locus_tag="PK281_10420"
     CDS             3481..>3831
                     /locus_tag="PK281_10420"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOY49799.1"
                     /translation="MIDLTLSPFIGILILSGLALLVQLYFYLFGFLRLARFKKNESTF
                     RKPPVSVVICARNEEENLKKFLPLVLDQDYPEFEVIVVNDCSWDNSYDLLKEMAVSSK
                     RLKVADIKEVEGREH"
BASE COUNT         1243 a          795 c          650 g         1143 t
ORIGIN      
        1 aacagattat taggagggta agcaacaaag ccataaccgc tcttcatatt taaggtttta
       61 gatactttca tatctccctt atctatgata ttctcgtagg tttttggagc actttcttga
      121 gtaacgaaca agccaccaac aattaattca ttgccgctag ctctgctatc aataatttcg
      181 tgcatcgcta ctggataggt cacttcttcc aataaatctt gtgaagtacg agtgatgatt
      241 tcacgtccag tatcgtcggt atattcaaaa tcccaactca ataccatcac acgtgttcca
      301 atggtattta ctttacgaat aagtggaaca aaatcgccat cagaagtaat caatacgaga
      361 acatcgtatt tcttcatgta gctcaattca agtgcttcta aagccagcca cacatcaatt
      421 cctttttcct ctttacggcc agcacgtgtt cttaatggaa ggtaatgcgt tactacgcct
      481 tccgacatta acacatcatc aaacaatcgg tcgttgtaca attgattacc gcgttgacta
      541 gcttcgtaag cactcattct cccccggaaa taatgagcat ctaccacatg acaaagacta
      601 taatccactt gcatctcttc cgcaatttgc tgacgaataa agctgtgcaa tcctgcaatg
      661 cttatacggc ggcgacgctg atggaaatag ttataataat tacttacgtg cagaaaataa
      721 ttcccgtcgt aaaaaacgcc aatacggaca actccgtctc ttggttgaag cataatttaa
      781 ttgtatttag atggttgaaa tggtttgttt taaaacgaat taattcaatt cgttaacctg
      841 tattaatctt aagaaatcgc cttgctgaaa tactgctgtg cgtagcagtt tatataagcg
      901 tattcgtctg aataataaca gaattaatga atggctgaac caaccgtctt aattaatatg
      961 ggcaaagata gcaaacagct aaatagctta atcaaaacaa acaaaaaaaa ggtagaaaag
     1021 tttatttcta attaacaatt aatattcagc aatcaaacga aatcccttat tacaagcaaa
     1081 tacaagatta tatgacaaag caaacaggaa ttcacattaa atgaatttaa ttccaacgag
     1141 gaaacatttc gatcaatcga aagctattta ggtgctttat agatgagacc aagtgcaaca
     1201 aaaaagtaaa ttaaattcgg tatccatgca gataatgctg gattcacgcc gccttcggtt
     1261 gcaaaaacag aagataattg catgagcatt acaaatgaaa aactgattcc aatacctatt
     1321 cctatatgta aaccaactcc tcctctaacc tttcgcccag ctaatggaac agcaatcaag
     1381 gctaaaataa tattagaaat aggatttgcc attcgacgtt gtcgttcaag taaataataa
     1441 tcgataaact ccgaaccttt gttctgttct tgaacgatga aacgattgag ttcaaaaaaa
     1501 ttcatcattt caacatcatc atcagcaatt gcaaaatcgg ctggggtaaa taataatgta
     1561 gtatcgagta atgcaacttt tctaatactc tccttctttc caataaattt ccttatcact
     1621 aaattatcca tcttccaact cgaagaagtt gagtcccaac gaatatagtc ggcactcatt
     1681 ttatacgtca ttttctgatc tttaaatcct tcttgagtaa atcgaaaacc tgtaactgaa
     1741 tgttgtgtat aggtttcgag atagacaaac gtccctggag caatctgacg gtgaagatta
     1801 ctaaagtttc gattccaatt atatttcagg tacttgtact cgaattttaa tcgtcgagca
     1861 tttgcaggag gaatgataaa acttcctagt aaaaacgaaa aaagcgcgac aacaattgcc
     1921 gaaatcataa atggtctaag aaatcgctta aagctgacgc cactacttaa aatcgcaacg
     1981 atttctgttt gattggccat acgtgaagtg aaaaacacca cactaataaa accaaaaata
     2041 ggagtaaata aattcgcgaa atagggaatg aaattcagat aataatcaat tagtaattcc
     2101 tgaatgggta tgtctttctc taggatgtca tccatttttt ccgttaaatc gaaaatgacg
     2161 ggtaacaata ctccaaacaa caatacagca tagaaaaaag tcccaaggaa tttttttatt
     2221 atatacttgt cgagaagata caaacgaata ttcataaacg acggctcact tttttcacca
     2281 tttcactttt ccaagtggca aaacttccgt ccaaaatacg tcttctagat tcttccacaa
     2341 gccaaagata aaatcccaaa ttatgcacac tggctatcat accagcaaga tattcacctc
     2401 caataaacaa atgtcttaaa taggcttttg tatactgctg atcggcaaaa gtgatgagta
     2461 aaggatcaat tgcggaaaaa tcagttttcc acttttcatt cctaatattg ataatgccct
     2521 cgctcgtaaa caacatccca tttcttgcat tacgcgttgg catcacacaa tcaaacatat
     2581 ctacaccttg tgagattcct tcaagaatat tagctggagt tccaactccc atcaaataac
     2641 gaggcttatc ggctggtagt attgaacata ccaactcagt catctcgtac atatcttcaa
     2701 ccggttctcc tactgataat cctccaatgg catttccatc tcgattttgc gcagcaataa
     2761 actcagcact ttgaatgcgt aattctctgt tggtacttcc ttgaacaatc ggaaataaag
     2821 tctgacgata cccatacaag ggttcttgct tatccaaatg ttcgatacaa cgctttaacc
     2881 agcgatgtgt gcgatccatg gaatgcttcg cgtaatcata tgtacaagga taaggtgtac
     2941 attcatcaaa tgccatgatg atatcggctc caatcgtacg ttgaatatcc atcaccgatt
     3001 cgggactgaa catgtgtttt gaaccatcaa tatgcgactt aaacatcgcg ccttcttcgg
     3061 ttatcttacg attatcggat aaagaaaaca cctgatatcc tccactgtcc gttaaaatag
     3121 gtctatccca gccattaaac ttatgtaagc cacctgcttg acgaagaaca tcaagtccgg
     3181 gacgtagata taagtggtag gtattaccta agatgattcg agcattgata tcatctcgaa
     3241 gttcacgttg atgcacggct ttaaccgtac cagcagttcc aaccggcata aatatcggtg
     3301 tgggtatttt accatgatct gttgaaattt ctccagctct ggctttggaa tgcttatctg
     3361 ttgctgcaag tgtaaagtgc atgctttgtt aaaaagttgc gcaaagatag accaatccat
     3421 cgcatctgag tcgattccat ccacctactt ttgtagctct ttttctgtgg cgattccgca
     3481 atgattgatc ttaccttatc accttttata ggcattctaa tactgtcggg gcttgcgctg
     3541 ctggttcagt tgtattttta tttgtttgga tttctccgac tagcccgatt caaaaaaaac
     3601 gaatcgactt ttcgtaaacc acccgtttct gtggttattt gtgctcgcaa cgaagaagaa
     3661 aacctgaaaa aatttcttcc attagtgctc gatcaggatt atcccgagtt tgaagtgatt
     3721 gttgtaaatg attgttcatg ggacaattct tatgacttac tgaaagaaat ggctgtatcg
     3781 agcaaacgac ttaaagtggc cgatattaaa gaggttgaag gtcgtgagca c
//
LOCUS       DAOOQW010000228         3797 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_28726_length_3797_cov_4.280866, whole genome shotgun sequence.
ACCESSION   DAOOQW010000228 DAOOQW010000000
VERSION     DAOOQW010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3797
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28726_length_3797_cov_4.280866"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1194)
                     /locus_tag="PK281_10425"
     CDS             complement(<1..1194)
                     /locus_tag="PK281_10425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49800.1"
                     /translation="MKNFATLLAILSATFSFAQTGNNTCASAIQICLTQPVTYPAAIN
                     AGAAEAGPDYGCLGSENNPAWFLFRIGTPGNHTITETNSNARDLDYILFGPFTNPTGN
                     CDSLDAAHTAACSYSGGATEIIDFISINSGEYYLLLVTNYSNLATNVTFTQTDGTGDY
                     DCEFEPICAVSLVTATPSACDTATNQYSVSGSVFTFNPPPTGTLTISDGIQDQVINSP
                     FNSSSNFTLNNLPSNGASVTLTAVYSAANGCQGNVIFTAPEGCLPCDVTVEATSTICE
                     GANLNFTTTFAGNATYQWTGPNSYSSTEMNPVINNISQAQAGTYTVLVTGTNCVAQRD
                     VIIDVIAAQRPQIIDIGTTVCEGDILFLSALEVPGGVFQWNGPNSFTADGRNAQVDDI
                     TPAGAG"
     gene            complement(1233..1817)
                     /locus_tag="PK281_10430"
     CDS             complement(1233..1817)
                     /locus_tag="PK281_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014790759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HOY49801.1"
                     /translation="MQLLKKLAGTEIILASGSPRRQELLNGLGIPFRVMKLDVEEQFP
                     DKLQRESIPLFLARLKAEEAKKNIQDNQLIITADTVVWLGDHVLNKPADLQEARWMLN
                     RLSGKTHEVITAVSLTSSKKSLSFYVVTEVVFRNLSEDEIMYYTETYKPLDKAGAYGI
                     QEWIGLVGVERIEGSYFNVMGLPTKELFEELLAF"
     gene            complement(1821..2816)
                     /locus_tag="PK281_10435"
     CDS             complement(1821..2816)
                     /locus_tag="PK281_10435"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglycerol-phosphate
                     geranylgeranyltransferase"
                     /protein_id="HOY49802.1"
                     /translation="MTDFLRLIRLPNLIIIALTLCGVRYGLMEPVWRHAIGDMLQQGY
                     MVQGLTLHMSLNDFILLICSIVLIAAAGYIINDYFDTRIDRINKPEQVIVGRVIKRRV
                     AMALHLSLSSLGLLIGIYLSFKVGNWKLSFIHLFSIIALWFYSTHLKKQLLSGNLLIS
                     LLAALVPITAGLYEFASGSIINLNILNAYVDGMGNSLLRKGAFLVIGYSAFAFLSNLI
                     REIVKDIEDMEGDQADGAQTLPLAVGETQAKFISICVVVFTIILLAFIQKQLWLNGFV
                     SMFWYILFAVQIPFLVLIIQIWRALQKHDYTLASRICKLVIVTGVLSMFVFRYTY"
     gene            complement(2900..3412)
                     /locus_tag="PK281_10440"
     CDS             complement(2900..3412)
                     /locus_tag="PK281_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008619455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HOY49803.1"
                     /translation="MQEKLKQIRALLFDIDGVLTDGSVTLMPDGTQIRNLHIKDCYAL
                     QLAVKKGFQVMIISGGKSEMMRERLASFGIEHIYMSVSRKIERYEDLLIECDLQESEC
                     LYMGDDIPDYEVMQRCGFAACPADAAQDIRAIAHYVSTAAGGKGAVREVLEQVLRAQD
                     KWMDADAFVW"
     gene            complement(3412..>3797)
                     /locus_tag="PK281_10445"
     CDS             complement(3412..>3797)
                     /locus_tag="PK281_10445"
                     /inference="COORDINATES: protein motif:HMM:NF022188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HOY49804.1"
                     /translation="ASDEFAHDELSELAKNLGGPFAWLTGEQRRMLHLAAVGANNFVN
                     HLMVLTEEWCLANGLDFQFLMPMIEQSAQRFKTSSPQLTQTGPASRNDQSTIALHLSM
                     LKNFPEFAEVYALLSKQISERSKRK"
BASE COUNT         1143 a          781 c          806 g         1067 t
ORIGIN      
        1 tccggcacca gcaggtgtaa tatcatcaac ctgagcattt cttccatcgg ctgtaaatga
       61 attggggcca ttccattgga aaactccacc cggaacttct aaagcactta ggaataaaat
      121 atccccttca caaactgtag ttccaatatc aataatttga ggacgttgag cagcaatcac
      181 atcaattata acatcgcgtt gagcaacaca attggtacct gtaaccaata ccgtatatgt
      241 tccagcttga gcttgggata tgttattaat caccggattc atttcggtgg aactgtatga
      301 attaggtccc gtccactgat aagttgcatt tccggcaaaa gtggtggtga aattcaagtt
      361 agcaccttcg caaatagtag aggtggcttc aacagttaca tcacatggta aacaaccttc
      421 aggtgcagta aaaataacat tcccttggca tccattagcg gcactataaa cggctgttaa
      481 tgttacgctt gcgccgtttg atgggagatt gtttagggtg aaattactcg aactattaaa
      541 aggagaattt attacttgat cttgaattcc atccgaaata gtcaatgtac ctgttggagg
      601 aggattgaac gtgaatacac ttccacttac cgaatattgg ttggttgcag tatcgcaagc
      661 agatggtgtg gcagtaacta aactaacagc acaaataggt tcaaactcac aatcgtaatc
      721 acccgttccg tcggtttggg taaaggttac gttggttgca aggttggagt aatttgtcac
      781 taacaataag tagtactcgc ccgaatttat ggaaatgaaa tcaatgattt cagtcgctcc
      841 accagaatag ctacatgctg cagtatgagc ggcatctaat gaatcacaat tacctgttgg
      901 attggtgaat ggaccaaata aaatataatc caaatcgcgt gcattggaat tcgtttcagt
      961 aatagtgtga tttccaggtg taccgatacg gaaaagaaac caagcaggat tattctcact
     1021 tcctaaacaa ccataatcag gtccggcttc agctgctcct gcattaattg ctgcagggta
     1081 tgtaacgggt tgagttaagc aaatttggat agccgaagcg caggtattat ttcctgtttg
     1141 ggcaaaagaa aaagttgccg acaaaatagc cagcagtgta gcgaagtttt tcattctggt
     1201 aagaaaggct gcaagttaac aattgccctg aattagaacg cgagaagctc ctcaaacaat
     1261 tctttggtag gtaatcccat cacgttaaaa tacgaacctt caattcgttc aactccaacc
     1321 aatccaatcc attcttgaat tccgtatgca ccggccttgt cgagcggttt gtaggtttcg
     1381 gtgtaataca tgatttcgtc ttcggaaagg tttctaaaaa cgacttcagt aactacataa
     1441 aaggaaaggc ttttcttgct cgaagtaaga ctaactgcag taatcacttc gtgtgttttt
     1501 cctgatagtc gattaagcat ccaacgtgct tcctgcaaat cggcaggttt atttaaaaca
     1561 tgatctccca accaaacaac ggtatctgca gtgatgatga gttgattatc ttgaatattc
     1621 tttttggctt cttcggcttt gagtcgggcg agaaaaagtg gaatggattc gcgttgcagc
     1681 ttatcgggaa attgctcttc tacatcaagt ttcatcactc gaaaaggaat tcctaaaccg
     1741 ttaagcaatt cttgtcgtcg aggtgatcca gaagcgagta ttatttcggt gccggcgagt
     1801 tttttcagga gttgcatgac ttaatacgta taacgaaaca cgaacatgga aagaactcct
     1861 gttacaatta ccagtttgca aatacgtgaa gctagtgtat aatcgtgttt ttgtaaggct
     1921 ctccaaattt gaataatcag gacaaggaat ggaatctgaa cagcaaaaag aatgtaccaa
     1981 aacatactga caaagccatt caaccaaagc tgtttttgaa taaatgccaa aaggatgata
     2041 gtaaaaacta ccacacaaat gctgataaac ttagcttgcg tttctcctac agccaagggc
     2101 aatgtttgag caccatcggc ttgatcgcct tccatgtctt cgatgtcttt tacgatttct
     2161 cgaatgaggt tgctcaaaaa ggcgaaagca gagtaaccaa tgactaggaa tgcacctttt
     2221 cgaagtagac tatttcccat tccatcgaca taagcattga gaatatttag gttgatgatg
     2281 gaaccagaag cgaattcgta caatccagca gtgatgggaa ctagagctgc taaaagtgat
     2341 ataagtagat tgcccgaaag gagttgtttt tttaagtgtg tggaataaaa ccacaaggct
     2401 ataatgctaa acaaatggat gaacgataat ttccaattcc ccactttgaa cgacaaataa
     2461 ataccaatga gtaatcccaa actactcaag cttaaatgca aagccattgc tactcgacgt
     2521 ttgataacgc gtcctactat aacctgttca ggtttattga tacggtcgat gcgtgtgtcg
     2581 aaatagtcgt tgatgatata acctgcagct gcaattaaaa cgatggagca gataagcaga
     2641 ataaaatcat tgaggctcat gtgcaaggtt agtccttgaa ccatataacc ttgttggagc
     2701 atatcgccaa ttgcgtggcg ccagacaggt tccattaatc catatctcac accacataag
     2761 gttaatgcga tgatgatgag attgggtaag cgaatgagtc gaaggaaatc cgtcatagcg
     2821 gtttcaaaaa tagaagaaat tctgccattc gattgtcatg gatgttaagg caatgagaac
     2881 atttaataac aagtatcgcc taccaaacga atgcatctgc atccatccat ttatcttgag
     2941 cacgaagtac ctgttcgagc acttctctta cagcaccttt tcccccagca gctgtactaa
     3001 cgtaatgtgc aattgcccta atatcttggg cagcatcggc cggacaagca gcaaaaccgc
     3061 agcgttgcat gacttcataa tcgggaatat cgtcgcccat atagaggcat tcggattctt
     3121 gcaggtcgca ttcgataagg agatcttcgt agcgttcgat tttacgggaa acgctcatgt
     3181 agatgtgttc aatcccaaaa ctggcgagac gttcgcgcat catctcggat tttccaccag
     3241 agattatcat cacctgaaaa cccttcttga cagccaattg tagtgcgtaa caatctttga
     3301 tgtgcaggtt gcggatttgt gttccatcgg gcattaatgt aacactacca tcggtgagta
     3361 cgccatcgat atcgaagagt agggcacgta tttgcttgag tttttcctgc attactttcg
     3421 tttgctacgt tccgaaattt gtttgctcaa taaggcgtaa acctctgcaa attcagggaa
     3481 attctttaac atgctcaaat gcaaggcaat ggtagattga tcgtttctac tcgccggtcc
     3541 cgtttgtgtg agttgaggtg aacttgtctt aaaacgttgt gcactttgct caatcatggg
     3601 catcagaaac tgaaaatcga gaccgtttgc taagcaccat tcttcggtga gtaccattaa
     3661 gtggttgacg aaattattcg caccaacagc agctaaatgg agcatgcggc gttgctcgcc
     3721 tgttaaccat gcgaatggac cacccagatt cttagctagt tcagataatt catcgtgagc
     3781 aaattcatcg gaagctt
//
LOCUS       DAOOQW010000229         3786 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_28848_length_3786_cov_4.703565, whole genome shotgun sequence.
ACCESSION   DAOOQW010000229 DAOOQW010000000
VERSION     DAOOQW010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3786
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28848_length_3786_cov_4.703565"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            149..637
                     /locus_tag="PK281_10450"
     CDS             149..637
                     /locus_tag="PK281_10450"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOY49805.1"
                     /translation="MKNKAVFLDRDGVVNRELGDYILRYEEFEILPELIPFLYEVKQR
                     GYKVIIITNQAGIAKGLYDHKLVDACHTFLIEELAGHGLQIDEIYYCPHHPDFGNCLC
                     RKPKSLLIEKALARFELDPSVSLMIGDKPRDVEAANGAGVRGFLVPPNPTLDQLLECL
                     NS"
     gene            634..1476
                     /locus_tag="PK281_10455"
     CDS             634..1476
                     /locus_tag="PK281_10455"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HOY49806.1"
                     /translation="MNKLICFNGTFLPEGEWMMGTSNRGFRYGDGVFETIRMQSGQLF
                     FVDDHFERLVGGMYTLGLDTTFLNPIQLCNEMLELCSRMNFAQARIRICIFRNDGGNY
                     RPTTDLCSYLITADPLENPIYTINEKGLRLGLYSEIRKPVNSLSGIKSSNALLYVLAA
                     RYARDNGWDDAVLLNEYGRICEATASNIFLVLPDKRILTPPVAEGILPGVMRKNLLRW
                     LRENGHQVEEAILLTDDFYRADEVFLSNSIQGISWVIGFRERRYFSSFSRSLVDALNT
                     RVSV"
     gene            complement(1473..2027)
                     /locus_tag="PK281_10460"
     CDS             complement(1473..2027)
                     /locus_tag="PK281_10460"
                     /inference="COORDINATES: protein motif:HMM:NF014659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HOY49807.1"
                     /translation="MNLLPPDKGRILLSEPFLNDSFFKRAVILLAENSDKGSMGFIMN
                     KPVGYQIHELISEFPEFESPVYFGGPVESNSLFFIHTVPHLIDSAFEIGDGIHYGGNY
                     EKLRNLAGMGLIQPDQVRFFAGYSGWDTGQLDFEMEEDSWLVFERPKTLLDMRPESLW
                     SELLKQSHSELAIWSNYPEDPTLN"
     gene            2217..2858
                     /gene="pdxH"
                     /locus_tag="PK281_10465"
     CDS             2217..2858
                     /gene="pdxH"
                     /locus_tag="PK281_10465"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014200997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HOY49808.1"
                     /translation="MQDRIQELRKEYRKEVLDEAATHANPIIQFKKWFQEALDVQLNE
                     PNAMMLATVNEHGRPSARIVLLKDITDIGITFYTNYLSRKGKEIAGQEYACVTFFWPE
                     LERQIRFEGKLSKVDAKISDDYFAIRPRGSQIGAWSSPQSQQIQSRAVLEENERKFIQ
                     QFEGKPVPRPDHWGGYQLEPDYVEFWQGRENRMHDRICYSLKEATWNRFRVAP"
     gene            complement(2860..>3786)
                     /locus_tag="PK281_10470"
     CDS             complement(2860..>3786)
                     /locus_tag="PK281_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase family protein"
                     /protein_id="HOY49809.1"
                     /translation="VSYGGGTAEGPAAILEASLQVDLYDPDSPEAWKSGIHLLPEDEE
                     IKEQSLVLRDRAELHIEALSKGTLQEHGEELLATISSACEQMNSSVESVCSKWLDAGK
                     LVGLLGGDHSTPLGFMQALAKRYPSFGILQIDAHMDLRIAYEGFTYSHASIMYNALKI
                     PQVTQLVQVGIRDYCEEEIHVMKNHSPRVKTFFDDQLKFNAYNGTTWKDQVAEIISHL
                     PQHVYVSFDIDGLDPKLCPNTGTPVAGGLEYHQAVYLLGELARSGKEIIGFDLNEVAP
                     GEDEWDANVGARLLFKLCVFAGISKGLFQAAR"
BASE COUNT         1110 a          744 c          808 g         1124 t
ORIGIN      
        1 aacagatcgt cttgaaacgg ccgatccaaa cgacccgcga actcgtcgat gcgcatctct
       61 ggggtggatt ctatggaatg tttctcacgc tggtactcag tcctgacatt gctcgtaatt
      121 tcattagtca aatcaccgat ttcatccgat gaagaataag gccgtttttt tagaccgaga
      181 tggggtagta aatcgggaat tgggcgatta tattcttcga tacgaggaat ttgaaattct
      241 accagagctt attccatttc tttacgaagt gaaacagcgt ggttataaag taatcatcat
      301 caccaatcaa gctggaatag ctaaaggact ttacgatcat aaattagttg atgcctgtca
      361 tactttctta atcgaagaac ttgcaggaca tggtttgcaa atagatgaga tttactattg
      421 tccacatcat cccgatttcg ggaactgctt atgtcgaaag ccgaagagtt tgctaattga
      481 gaaagcgctt gctcgtttcg aacttgatcc ttcagttagt ttgatgattg gtgataaacc
      541 tcgagatgtg gaagctgcga atggtgctgg tgttcgtggt tttttagtgc cgcccaatcc
      601 tactttagat caactacttg aatgcctgaa ttcatgaata aactgatttg tttcaatggt
      661 acgtttttac ccgaagggga atggatgatg ggcacatcaa accgtggctt tcgttatggt
      721 gatggcgtgt ttgaaacaat acgcatgcaa tcaggtcaat tgttttttgt ggatgaccac
      781 tttgaacgtc ttgttggtgg gatgtataca ttaggtctcg acactacctt tttgaatccg
      841 attcagttat gcaacgaaat gttggaattg tgcagtcgga tgaattttgc tcaagctcga
      901 atccggatct gtatttttcg aaatgacggt ggaaattatc gtccaacaac cgatttgtgt
      961 agctatttaa tcacggctga tcctttagaa aatcccatct atacgatcaa tgagaaaggc
     1021 cttcgtttag gcttgtattc tgaaattcga aagccggtaa atagtttgtc gggtattaaa
     1081 tcttctaacg ccttactcta tgttcttgca gcgcgttatg cccgtgataa tggttgggac
     1141 gatgcagttt tattgaacga atatggtcga atttgtgaag caacagcttc caatattttc
     1201 ttggttttac ctgataaacg catattaact cctcccgtgg ctgaaggaat tttaccgggt
     1261 gtgatgcgga aaaacctatt aagatggttg agagaaaatg gacatcaggt agaagaagcc
     1321 attttattga ccgatgattt ttatcgggca gatgaagtat ttctaagcaa ctcgattcaa
     1381 ggaattagtt gggttatcgg atttagagaa agacgctact tcagttcatt ctctaggagt
     1441 ttggttgatg ccttaaatac tcgagtatcg gtttagttca atgttggatc ctctggatag
     1501 tttgaccaaa ttgctaattc gctgtggctt tgtttaagca attcgctcca aagtgattcg
     1561 ggacgcatgt cgagtaaagt tttagggcgc tcaaatacca accaagaatc ttcttccatt
     1621 tcgaagtcta attgaccggt atcccaaccg gaatatccag caaagaaacg aacctgatcc
     1681 ggttgtatca atcccattcc tgcaagattc cttagttttt catagttccc accatagtga
     1741 atgccatcgc caatttcaaa agcgctatca ataagatgtg gaacggtatg gatgaagaat
     1801 agcgaattac tttctacagg tccaccgaaa tacactggac tttcaaattc agggaattcg
     1861 ctaatgagtt catgtatctg atagccaacc ggtttattca tgataaaacc catcgaacct
     1921 ttatctgaat tctcagctaa aagaataaca gctcgcttaa aaaatgaatc atttaaaaag
     1981 ggttccgaaa gcagtatcct gcctttatcg ggagggagca ggttcatttt catgggtgca
     2041 tttttttaat agaacggtaa taggaaataa aaagttactg aatgccccgc tgatagttca
     2101 atacaaaaaa ttgacattgt caggaaaaac cagattcttg ttacctgaat aatcctgata
     2161 aagatcagtt atggtttgat tcaaattgct caatttcgct gcataaaatt tcgattatgc
     2221 aagatcgtat tcaggagtta agaaaagaat atcgcaaaga agtgttggat gaagcggcga
     2281 cacatgcaaa tccaattatt caatttaaga agtggtttca agaagccttg gatgttcaac
     2341 tgaacgaacc taatgctatg atgttggcta ctgttaatga acatggaaga ccatcggcac
     2401 gcatagtttt gctaaaggac ataaccgata ttggaattac tttttatacc aattacttaa
     2461 gtcgaaaagg aaaggagata gccggacaag aatacgcatg tgtcacattc ttctggcccg
     2521 agttagagcg ccagattcga tttgaaggta aattgtcaaa agtagatgcg aagatttcgg
     2581 atgattattt tgcaatccgc ccacgcggca gtcaaattgg agcttggtct tcaccacaaa
     2641 gtcagcaaat tcagtcacgt gctgtattag aagaaaacga acgcaagttt attcaacaat
     2701 ttgagggcaa gcctgtacca cgtccagatc attggggcgg gtatcaatta gaacctgatt
     2761 atgttgaatt ctggcaaggg cgcgagaatc gtatgcacga ccggatttgt tacagtttga
     2821 aagaagctac ttggaatcgt tttcgggttg ctccttaaat taacgagctg cttggaataa
     2881 acctttagaa attccagcaa acacacatag tttgaacaac aaacgggctc cgacattagc
     2941 atcccattca tcttctccag gggcaacttc attcaaatcg aagccgataa tctcttttcc
     3001 acttcgagcc agttcaccca ataaatacac agcttggtga tattctaatc caccggcaac
     3061 tggagtaccc gtatttggac aaagcttagg atcaagccca tcgatgtcaa agctaacata
     3121 tacgtgttga ggcagatggc taatgatttc agcaacttga tctttccagg ttgtaccgtt
     3181 gtatgcgtta aatttgagtt gatcgtcgaa gaacgttttt acacgaggtg aatgattttt
     3241 catcacatga atttcctctt cgcaataatc acgaattcca acttgaacta actgagttac
     3301 ttgaggaatc ttgagtgcat tatacatgat tgatgcatgt gagtaggtaa agccttcgta
     3361 ggcaatacgt aagtccatgt gagcatcaat ttgaaggata ccaaaggaag gatatcgctt
     3421 cgctagagcc tgcataaacc ctagaggagt gctatggtca ccacccagta gaccaacaag
     3481 tttacctgca tccagccact ttgaacatac cgattctact gaggaattca tttgttcgca
     3541 agcagagcta attgtagcaa gtaactcttc cccatgttct tgaagagtcc ctttcgacaa
     3601 ggcttcaata tgtaattccg cccgatcgcg taggactaaa ctttgttctt ttatttcttc
     3661 atcctcggga agtaggtgaa tacctgattt ccatgcttcg ggagaatctg gatcgtataa
     3721 atcaacctga agactagctt ccaaaatagc tgccggacct tcggctgttc caccaccata
     3781 actaac
//
LOCUS       DAOOQW010000230         3756 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_29151_length_3756_cov_4.321805, whole genome shotgun sequence.
ACCESSION   DAOOQW010000230 DAOOQW010000000
VERSION     DAOOQW010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3756
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29151_length_3756_cov_4.321805"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(353..1378)
                     /locus_tag="PK281_10475"
     CDS             complement(353..1378)
                     /locus_tag="PK281_10475"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOY49810.1"
                     /translation="MSLFLAFWFSVCVGFLVGIFEYLFHKHYTIWVPVVVSTLTFILS
                     FFLFQFAVERFIYRKIKLIYKNIHQLKLKKGQKSKSVDLNTDIIAAVNKDVEAWAKDQ
                     KEEIEYLKNLENYRREFLGNVSHELKTPIFNIQGYIMTLLEGGLEDPSINRTYLERAE
                     KSVERMVNIVEDLEIITQFESGQLILHKEKFDIVELARDTMSSIQYSAEKNNVKLVFK
                     EVYDKPIYVKADKESVQKVLSNLLVNSIKYSKQVGETKLSFYDMDENILVEVTDNGIG
                     ISQEHIPRLFERFYRVDRSRSRDIGGTGLGLSIVKHIIEAHNQTINVRSSLGIGTTFG
                     FTLAKSR"
     gene            complement(1434..2114)
                     /locus_tag="PK281_10480"
     CDS             complement(1434..2114)
                     /locus_tag="PK281_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOY49811.1"
                     /translation="MSTEKTKILLVDDEPDILEFISYNLTKEGFEVFTSGNGKDAIRL
                     AQLERPHLIILDVMMPDLDGIETCRVIREIPEMKDVLIAFLTARNEDYSQIAGFDAGA
                     DDYINKPIKPRVLVSRVKALLRRKGNVQEEEVMDVNGFMIDREKYLIVKDNVQINLPK
                     KEFELIALLASRPGKVFTREDILKNVWGDDVVVGDRTIDVHIRKLREKLGDAYIRTIK
                     GVGYKFEF"
     gene            complement(2254..3600)
                     /locus_tag="PK281_10485"
     CDS             complement(2254..3600)
                     /locus_tag="PK281_10485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOY49812.1"
                     /translation="MSLPKTSSYGHIMRLAMPIIVGGIASNVILATDAFFMARVDEVS
                     LAAVGLAGLFYTTIYILGLGFSTGVQILIARRHGEKKYHAIGAIFDNSLIFLLGMALI
                     LWLGMEFLGPPLLKSLVKSPEVYRSAVVYLDNRAWGITFALANLAYRAFFIGVSSTGV
                     IIWSTFAMAISNVILNYGLIFGNFGLPEMGIAGAAVASSLAEIIGLIWFALHAYLGKY
                     HQTFDLFHSWKWQPDVMKTISSIASPVMFQNFVSHAGWFLFFIIIEQSGERALAVSVI
                     IRIIYMFQMVPFWGLSSASNTLVSFTIGEGRYQDVLPLLKRITWLSLALVSPFLLLNL
                     FVPELILGLAVENKNATGLISDSIPTLYVITLALSFFGPAMTWYSGVTGSGNTRTSLL
                     IETITIASYLLLAWFLGIFIKADVHVIWFTEPYYFLVLWFVSWIYMRQGTWKNKKV"
BASE COUNT         1183 a          787 c          725 g         1061 t
ORIGIN      
        1 cagaattagg agcccagttc gagtttatag tttgagcatt aagcccaaat gatgcagatg
       61 caaagacagc tgctaataga gtaacgtgtt tcatgcgttt ttttcttaaa gttattttaa
      121 agtgttgtgt tgtaattggg agagaaatca atatgtgaac aagcaaatgt gtattatgat
      181 tccacgcacg ctcataacgg tttaatacct atagagttac taatataagg ctttgtagct
      241 tacatcaagt gtaagaaata gaagagaaaa taattacaga gaggctaact cgttcagaac
      301 aaacgatttt ccttcaagta tgatagtaat aagctgtaag aaaagatggc aattagcgcg
      361 attttgctaa agtaaatccg aatgtagtgc ctattccaag ggaacttcgc acgttaatgg
      421 tttggttgtg tgcttcgatg atgtgtttca caatcgacaa ccccaaaccg gttccaccaa
      481 tgtcgcgcga acgactacga tctacccgat aaaaacgctc aaacaaccgt ggaatatgtt
      541 cctgagaaat tcctattcca ttgtcggtta cctcaacaag gatgttctca tccatatcgt
      601 aaaacgaaag tttagtctcc cctacttgct tgctatactt gatcgaattc accaacaaat
      661 tactgagcac tttctgcacg ctttctttgt cggcctttac ataaataggc ttatcgtaaa
      721 cttctttaaa taccaatttt acattattct tctctgcact atactgaata ctcgacatcg
      781 tgtctcgagc tagttcaaca atgtcgaatt tctctttgtg gaggattaat tgtccagatt
      841 cgaattgtgt aataatctct aaatcttcca caatattcac catccgctca acactctttt
      901 cagctcgttc gagataggta cgattgattg aaggatcttc taaaccacct tctagcaaag
      961 tcatgatata accttgaata ttgaaaatgg gtgttttcaa ttcgtggctc acatttccga
     1021 gaaattcacg acggtagttt tcgaggttct ttaggtattc gatttcctcc ttttgatcct
     1081 tagcccaagc ttccacatct ttgtttaccg ctgcaatgat atcggtattg agatcgaccg
     1141 atttagattt ttggcctttc ttgagtttga gttggtggat gtttttataa atcagtttga
     1201 ttttccggta gatgaaacgc tcaacggcaa actgaaaaag aaaaaacgac aagataaatg
     1261 tgagcgtact cactaccacc ggcacccaga tggtataatg cttatgaaac agatactcga
     1321 aaatacccac taagaatccc acacataccg aaaaccagaa tgcgagaaac aaggacaaac
     1381 tgcgcggtgt agacgtattg agtcgttgcg cgaatgtgat ttggggtatt gctttagaat
     1441 tcaaatttgt atcctactcc ttttatagtg cgaatgtatg catctcctag cttttcacga
     1501 agttttcgga tgtgaacatc aatagttctg tctcctacaa ccacatcatc accccataca
     1561 ttcttcaaaa tatcttctcg agtgaatact tttccgggac gagaagccaa cagagcaatg
     1621 agttcaaact ctttcttagg aaggtttatt tgaacattat ctttcacaat taggtatttc
     1681 tcccgatcga tcatgaaacc attcacatcc atcacctctt cttcttgcac atttcctttc
     1741 cggcgaagta atgctttcac acgagaaaca agtacacgag gcttgatagg tttattgatg
     1801 taatcatcgg ctcccgcatc aaacccagca atttgagaat aatcttcgtt gcgagctgtc
     1861 agaaatgcga tgagcacatc tttcatctct ggaatttcac gtattactcg acaggtttca
     1921 atgccatcta aatcgggcat catcacatcc agaatgatca aatgtggtcg ttccagttga
     1981 gcaagacgta tagcatcttt cccatttcca ctggtgaata cttcaaatcc ttccttcgtt
     2041 aaattgtaac tgataaattc cagaatatcc ggttcatcat caacaagcag gatttttgtc
     2101 ttctcagtac tcataaagta tgtgttacga tacggttgcg aaattattct acttatgcct
     2161 cgtctaagtt aagttgacgt tatgaaattg acattattat ttcgggaatg ttgatttatt
     2221 tatcaaaatc caaagaatga actagttccg acattatact ttcttgtttt tccaagttcc
     2281 ttgacgcata taaatccagg aaacaaacca aagtacgaga aaataataag gctcggtaaa
     2341 ccaaattaca tgaacatcgg cttttatgaa tatgcctaga aaccaagcca gcagtaaata
     2401 agaagcgatt gttatggttt cgattaacaa agatgtacgt gtatttcccg aaccagtaac
     2461 accgctatac caagtcatgg caggaccaaa aaaagatagc gctaaggtga taacatataa
     2521 tgtaggaata ctgtcgctga ttaatcccgt tgcattttta ttctcaacag ctaaacccaa
     2581 aatcaattct ggaacgaata aattaagcag caagaaggga gaaacaagcg ctagagatag
     2641 ccaagtgatt cgctttagca gtggaagaac atcctgatat cggccttcgc caatggtgaa
     2701 tgaaacgagt gtattggaag ccgaactcaa tccccagaat ggcaccattt gaaacatata
     2761 tatgatacga atgatgaccg aaacagctaa ggcacgttcg ccactttgct caatgatgat
     2821 gaagaacaga aaccatccag catgagagac aaaattctga aacatgacag gagatgcaat
     2881 tgaagagatg gttttcatca catcaggttg ccatttccag gaatgaaata gatcaaacgt
     2941 ttggtggtat ttgcccaagt aggcatgaag tgcaaaccaa attaagccaa tgatttcggc
     3001 taaggatgaa gctacagctg ctccggcaat gcccatttcg ggcagaccaa aatttccgaa
     3061 aatgagtccg tagttgagaa tcacattcga aatagccatc gcaaacgtgc tccagataat
     3121 aacacctgtg ctagatacac caatgaaaaa tgctctgtaa gctagattag ccaaggcgaa
     3181 ggttatgccc caagcgcggt tgtcgaggta aactacagcc gaacggtata cttcgggcga
     3241 tttcaccaac gactttaaca gcggtggccc aagaaattcc attcccaacc aaagaattag
     3301 cgccattccc agtaggaaaa tcaagctatt gtcgaaaata gctccaatgg cgtgatattt
     3361 cttttccccg tgacgacgag caataagaat ttgtacgccc gttgaaaatc ctaatccaag
     3421 aatgtagatg gtggtataaa aaagtccggc tagtccaaca gctgctagtg aaacttcatc
     3481 cactcgtgcc ataaaaaagg catcagttgc cagaatcacg ttggaggcaa taccacccac
     3541 tatgataggc atagccaatc gcatgatgtg accgtaggaa gaagttttag gtaaagacat
     3601 ccaacaaatt tagcgcacta aaacgaccaa tcctttgagg tattcacctt cgggatggta
     3661 aatgcttacg ggatgatcag caggttgagt aagttggtgc aagatgacca cttctctacc
     3721 cgcctcaata gcagcagcag taacagtatt tcggaa
//
LOCUS       DAOOQW010000231         3728 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_29446_length_3728_cov_3.023414, whole genome shotgun sequence.
ACCESSION   DAOOQW010000231 DAOOQW010000000
VERSION     DAOOQW010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3728
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29446_length_3728_cov_3.023414"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1054
                     /locus_tag="PK281_10490"
     CDS             <1..1054
                     /locus_tag="PK281_10490"
                     /inference="COORDINATES: protein motif:HMM:NF022732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3089 domain-containing protein"
                     /protein_id="HOY49813.1"
                     /translation="MLRFSIFILFLSLIFSCNRPYTGKYTPPIVAKSFADCATPPAPD
                     YSNPDHWAALPTKKDMADSVPNRSVKDGQAQAQVDVFFLHPTLYLEPGEDKKYPWNAD
                     VNDVTMNAKVDGSTILNQASVFNGSCRVYAPRYRQAHISSFFTADSKSGEAALELAYQ
                     DTKAAFQYYLEHYNQGRPFFIAGHSQGTRHAGMLIRELIEGKPLANKLIAAYVIGMPV
                     PSDFFTQVPICDSPDQTGCYITWATYQKGFYPLNYESSGFNRGKCVNPLCWTTDSTYV
                     SRNKNLGGITWKFNKVKRRINGAQSNKGILWIEKPHVPGRMFIKMDNYHVADYNLFWF
                     NIRENVRLRTEAFIRK"
     gene            1160..3496
                     /locus_tag="PK281_10495"
     CDS             1160..3496
                     /locus_tag="PK281_10495"
                     /inference="COORDINATES: protein motif:HMM:NF013360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016641 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, oxygen as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysyl oxidase family protein"
                     /protein_id="HOY49814.1"
                     /translation="MRNIATFLLLFAFAPLFSQNCTTGFSEVIVKIIPDGYPDETSWE
                     IVNGGNVVASGTTNSDTVCVPNNSCVQVLIHDSFGDGIYSPGGYWIYLNGSEIATGSA
                     FGSEAIHEVACPLGSTCSQPLAISTGNWVTQFEDTWYTYTPSTSGMYVLSTCGTNTCN
                     TALWVFESCPIVGVTDGPMGSYTFNDDAAGCGQQSSVNVMMIAGHNYIIRVGDVNNNC
                     TSPITFSFNYNGPITGCTDQTSCNFNPLATVDDGSCLYFPDPGCQGPDLAFDSTAFIT
                     SLFLGTHTTSGCDISEDCVTGYGLRYVIEFSSKINNIGTTDYYIGTPSSQPGQFNTTN
                     CHGHAHYEGYGDYRLYDSNNNIIPAGHKNGFCVMDLCGFGQYSCGDMGISAGCYDIYG
                     AGTQCQWIDITDVPAGDYRLAVIINSHHLPDALGRHEINYINNALQVCIHISRDANNV
                     PSFTVLPDCDPFVDCAGTPGGMAIMDCNNECGGMAMYGNTVEDNIVNGQDLSGYFNMF
                     QQDNAPALPCYDLNSNDALTIYDAALEQWCLNSAGTGSGMSHCNFPRDVINPQDTSGL
                     AITNANFSSNYIDVELLSPRADIVGWQFRVSGIHMTDVVALTSEIDMPMLLGKNVSRG
                     EVFGLYNGDSILHRSETANAVCRIYFDQITEDKICIESIQDIPNSIGERTVTEIYGIC
                     FDKPLGINDMPQRAHLTIMPNPAKEMAWVNVPDGSNWNGSWELLDAMGRVVKKVTPSN
                     GSQAQLYAIQLTDLLQGVYFLRIQDKTGRSAIGRLVKN"
BASE COUNT         1083 a          783 c          762 g         1100 t
ORIGIN      
        1 catgcttcgc ttttccattt tcattctttt cctttccctt atcttttctt gtaatcgtcc
       61 ttatacagga aagtacacac ctcccattgt agctaaatca tttgccgatt gcgctactcc
      121 tcctgcaccc gattacagca atcccgatca ttgggctgct ttaccaacta agaaagacat
      181 ggccgatagt gtcccaaatc gaagcgtaaa agacggacaa gctcaagcgc aagttgatgt
      241 attctttctt cacccaacgc tttatcttga acccggagaa gacaaaaaat atccttggaa
      301 tgccgacgtg aacgacgtta ctatgaatgc caaagtagat ggcagcacga ttctcaatca
      361 agcaagtgtg tttaacggtt catgccgggt gtatgcgcca cgctaccgtc aggcacacat
      421 tagttcattt ttcacagctg attccaaaag tggtgaagcg gcactcgaac tagcttatca
      481 agatactaaa gctgcttttc aatattacct cgaacattat aatcaaggac gtccgttttt
      541 catcgctgga catagtcaag gaactcgaca tgcgggtatg cttattcgtg aattaatcga
      601 gggaaaaccg ctggcgaata aacttattgc tgcttatgtg attggtatgc ctgtacctag
      661 cgactttttc acccaagtac caatttgcga cagtcccgac caaacaggct gttacatcac
      721 ttgggcaaca tatcaaaaag gattttatcc acttaactat gaaagctctg gatttaatcg
      781 tggtaaatgt gtgaatccac tgtgctggac aaccgattca acctatgtat cgcgaaataa
      841 aaatttggga ggaataacct ggaaattcaa caaggtaaaa agacgcataa atggagctca
      901 atcgaacaag ggaattttat ggattgaaaa gccacatgta cctggtagga tgttcatcaa
      961 aatggataat tatcacgtag ccgattataa tttgttttgg ttcaatatcc gtgaaaacgt
     1021 acgtttaaga accgaggctt tcatccgtaa ataaagggat cagcccattt tacattagtt
     1081 ggacgaagca gattttcaaa tagttgattc tttatttcta taaattttag taggtttgaa
     1141 tgctaatcaa tctactacta tgagaaacat cgccacgttt ctattgcttt tcgcttttgc
     1201 accgcttttt tcccaaaact gcacaacagg cttcagcgaa gtaattgtta aaatcatccc
     1261 tgatggatat ccagacgaaa cttcttggga aattgtcaat ggaggaaatg ttgttgctag
     1321 cggaaccacc aatagtgaca ccgtttgtgt tcccaataat tcttgcgttc aggttttaat
     1381 tcacgattca tttggtgatg gaatttactc acctggcgga tactggattt acctaaatgg
     1441 ctctgaaata gctaccggaa gtgctttcgg ttctgaagca attcacgaag ttgcttgtcc
     1501 gctaggctca acctgcagtc agccacttgc tattagtacc ggcaattggg tgacgcaatt
     1561 tgaagatact tggtacacat acactccttc aacatccgga atgtatgtgc tttctacttg
     1621 cggaacaaac acctgtaata ccgcactttg ggtttttgaa agctgcccaa tcgtaggtgt
     1681 aaccgatgga ccaatgggtt catatacttt taatgatgat gctgcaggct gtggtcaaca
     1741 atcgtccgta aacgtgatga tgatagctgg tcataactac atcattcgtg taggtgatgt
     1801 gaataacaac tgtacctccc caataacttt cagctttaat tataacggtc caattacagg
     1861 ttgcaccgat caaacttcat gtaacttcaa tccattggct actgttgatg atggttcatg
     1921 cttgtatttc cctgatccag gttgccaagg tcccgattta gcattcgatt ctacagcatt
     1981 cattacttct cttttccttg gtactcacac cacatcggga tgcgatattt ctgaagactg
     2041 tgtcaccgga tatggcttac gctacgtaat tgagttctcc tctaaaatca acaacatcgg
     2101 aactaccgat tattacatcg gtactccttc atctcaaccc ggacaattca ataccaccaa
     2161 ttgccacgga catgcgcatt acgaaggtta tggcgactac cgtttgtatg attccaataa
     2221 taacatcatc ccggcaggac ataaaaatgg tttttgtgta atggatttat gtggatttgg
     2281 tcaatatagc tgcggcgaca tgggtatttc ggctggatgt tatgatatct atggagcagg
     2341 aactcaatgt cagtggattg atattacaga tgttccagcg ggagattatc gtttagctgt
     2401 aattatcaat tcccatcatc ttccagatgc attgggacgt catgagataa attatatcaa
     2461 taatgcactt caagtttgca ttcatattag ccgcgacgcg aacaatgttc catcttttac
     2521 agtgcttcct gattgtgatc catttgtaga ttgcgccggc acaccaggag gaatggcaat
     2581 catggattgt aacaacgaat gtggtgggat ggcgatgtat ggtaatacgg tagaagacaa
     2641 tattgttaac gggcaagatt tgagtggtta ttttaacatg ttccaacaag ataatgctcc
     2701 agctttgcct tgctacgact tgaatagcaa cgatgcctta actatttacg atgcagctct
     2761 tgaacaatgg tgtttgaatt ctgcaggaac tggttcgggt atgtcgcatt gcaatttccc
     2821 acgcgatgta atcaatccac aagacacttc tggattagcc atcaccaatg caaatttcag
     2881 tagcaattac attgatgtag aattactttc tccacgtgcc gatatcgttg gatggcaatt
     2941 ccgtgttagt ggtattcaca tgactgatgt agttgcccta accagcgaaa ttgatatgcc
     3001 gatgctttta ggcaaaaacg tttcccgagg tgaagtattt ggcttgtaca atggcgatag
     3061 tattcttcac cgttcagaga ctgccaatgc cgtttgccgt atctactttg atcaaattac
     3121 cgaggataaa atttgcatcg aaagcattca ggatatcccg aattcaattg gcgaacgtac
     3181 agtaacagaa atctacggca tctgtttcga caaaccactt ggcatcaacg atatgcctca
     3241 acgtgcacat ttaaccatta tgccaaatcc ggctaaagaa atggcttggg ttaatgtacc
     3301 tgatggttca aactggaatg gctcttggga attactcgat gccatgggac gagtggtgaa
     3361 aaaagtaaca ccttcgaacg gaagtcaagc tcaactttat gccatccaat taaccgattt
     3421 attacagggc gtatatttcc ttcgcatcca agataaaaca ggccgaagcg caataggacg
     3481 cttggtgaag aattaagaaa aaaagcctcg gaaaaaattc cggggctttt ttttgatgta
     3541 cgatgtacgt agtacgattt acgaaatctt tgctaggaca tgcattcgtt ttccaatgaa
     3601 actcctgtag cagaattata caacctagca gtttctctcg taaatcgtat atcgtaatac
     3661 gtaaatcgaa caacgaagcg atcgtaagtc agtattaagt ataaagtacg aagtataaag
     3721 aagatgaa
//
LOCUS       DAOOQW010000232         3727 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_29452_length_3727_cov_4.508987, whole genome shotgun sequence.
ACCESSION   DAOOQW010000232 DAOOQW010000000
VERSION     DAOOQW010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3727
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29452_length_3727_cov_4.508987"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1352)
                     /gene="sppA"
                     /locus_tag="PK281_10500"
     CDS             complement(<1..1352)
                     /gene="sppA"
                     /locus_tag="PK281_10500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HOY49815.1"
                     /translation="MKSFFKIVFASMLGFLLTFFVLFLIIAAIIGASSGEKEVKLEKN
                     SILHLTFDEPIKDRGNDNPFANFSFGSMEGGKQVGLNDILDNIAKAAADEHIDGIFLD
                     VTSVPSGIATIEEIRNELIEFKKSGKFIYAYSEGFTQPSYYLATAADKIYLHPTGMAE
                     LKGLRSEVLFFKGTLDKIGVEPQVFRHGKFKSAVEPFIQDKLSDANREQIRSYIGSIW
                     NHLVDGIAKERKITPAQVQDIASRMLVTNAPAAVQFKLVDELKYRDEVLEILQKKTNA
                     KGIDEIEFVSMGKMVDVKDTRDKSGKDKIAIVFAEGDIVDGEGNDGQVGSVEFSKAIR
                     KARLDEKVKAVVLRINSPGGSALASETIWREVMLTRKVKPVIASMGNVAASGGYYIAA
                     PCDTIVASPNTITGSIGVFGLFFNARQMVNNIGVTIDTVKTNPYADIMTASRAVTAQE
                     A"
     gene            1418..1915
                     /gene="folK"
                     /locus_tag="PK281_10505"
     CDS             1418..1915
                     /gene="folK"
                     /locus_tag="PK281_10505"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HOY49816.1"
                     /translation="MKEVGIGMGSNLGNRLEILQQAVSFIKELSGEDFRISSVYDSEA
                     WGYDSLNSYYNMVLVFRTFLSADDLLESLLEIEQRLGRVRVDKSYGDRSIDLDLLFYG
                     DLIRNDEHLILPHPRMVLRRFVLEPLQEIHSSWRHPQTGLMISTILSACEDSPIVKLR
                     HSVSE"
     gene            2072..>3727
                     /locus_tag="PK281_10510"
     CDS             2072..>3727
                     /locus_tag="PK281_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49817.1"
                     /translation="MNKFTKAGLLFIIPITLLGVASCGIKKMVKDAGKISYEAKPNPL
                     ELHGDSVKVSVTGNFPAKYFHKKATLTVTPVIKYTGGEKELKSIILRGDKVEGAGQNI
                     TYTEGGKFSVSDQFLYVTGMDKCEVILRSTAQYKSKTTTLPEVSIAKGTIVTPLMLKK
                     DYQASVAVDKFDKNPTVTQKANIYYLVDSWEVRNVELKSDESDNMNRFVERAAKDGSE
                     FTKLDIYGYASPEGELSRNSKLSENRADEAYKVMLAKFKKAKLKDLEKKEGFYSKITT
                     DYEDWDGLKKMLQTSSIAGKDEAMKVITSITDPEAREEEFKKMASFDPIYEAYFPKLR
                     RAEINLVAKIKTRTDDQIRVAAIASPDSLGMEELLYGSTLLDSDDDKLKVYQSYARRF
                     PEDFRGFNNTGVIYVKQGKINEAQQEFEKASKVAGNNAQVQNNLGVVAAMKGDRKAAA
                     QFFAAAGTTAEALHNKGNLAVASGKYADATSNYGSECSFNAALAKLLAGNASGATQTL
                     DCSDDKDSAEGFYLRAVIAARSNNKDGVVSNLTKAISAKSILKE"
BASE COUNT         1164 a          626 c          787 g         1150 t
ORIGIN      
        1 gcctcttgag cagtaacggc acgtgatgca gtcataatat cagcatacgg attcgtttta
       61 actgtatcga tagtaacacc aatattattc accatttggc gagcattaaa gaacaaacca
      121 aatacaccga tagaaccggt tatcgtatta ggactagcaa caattgtatc gcatggagca
      181 gcaatgtaat aaccgcccga agcagcaaca tttcccatag aagcaataac tggcttcacc
      241 ttgcgagtta gcattacttc tctccagatt gtttcggaag caagtgcaga tccacctggt
      301 gaattaattc ttaagacaac tgctttcact ttttcatcta aacgagcttt gcggattgct
      361 ttagaaaatt caaccgaacc aacttgtccg tcatttcctt caccatcaac aatatcacct
      421 tcggcaaata cgatggcgat tttatctttt ccagatttgt cgcgtgtatc tttcacatcg
      481 accattttgc ccatagaaac gaattcaatt tcatcgattc ctttagcatt ggttttcttt
      541 tgaagtattt cgaggacttc gtcgcggtat tttagttcat ccactaattt aaactggacc
      601 gcagctggag cattggtaac aagcatgcga ctagctatat cttgaacttg agcaggagtg
      661 attttgcgtt ctttagcgat accatcaact aggtgattcc agatagatcc aatataggaa
      721 cggatttgtt cgcggtttgc atcggagagt ttatcttgaa taaaaggctc tacggcactt
      781 ttgaattttc catgacggaa aacttgcggt tctactccaa ttttatctag tgttcctttg
      841 aagaaaagca cttctgagcg cagacctttt aattcggcca tgcctgtagg atgcaaataa
      901 attttatcgg cagcagttgc taggtaatag gaaggctgag taaaaccttc actataagcg
      961 tagatgaatt tacctgattt tttgaattcg attagttcgt tgcggatttc ttcgatggtt
     1021 gcaattcctg aaggaacact tgtaacatcg aggaatattc cgtcgatatg ttcatcggct
     1081 gcggcttttg cgatattatc gaggatgtcg tttaatccga cttgctttcc gccttccata
     1141 gagccaaagc tgaagtttgc aaatggatta tcgtttcctc gatctttaat aggctcatca
     1201 aaagtgaggt gaagaatgga atttttttcg agtttgactt ctttttcacc tgagctggct
     1261 ccaataatag ctgctatgat tagaaacaag acaaagaatg tgagcaggaa gcccagcatc
     1321 gatgcgaata ctattttaaa aaatgatttc atgggttgga aaatattaag tggtgcaaat
     1381 taggaaaatg tgaattggaa aatcttagtt tcgggtcatg aaggaagttg gaattggaat
     1441 gggtagtaat ctgggcaatc gcttagaaat attgcagcaa gctgtatcat ttataaaaga
     1501 gctttcgggg gaagactttc gcatttcttc tgtgtatgat tctgaagctt ggggatatga
     1561 ttcgttgaat tcctattata atatggtgct tgtttttcgg acatttttat cggcagatga
     1621 cttattagaa tcgttgttag agattgagca gcgactagga agggttagag tagataaaag
     1681 ttacggtgat cgatcaattg atttggattt gttgttttat ggggacttaa ttagaaatga
     1741 tgagcattta attttaccac atccacgaat ggttttgcgg agatttgtat tagaaccttt
     1801 acaagaaatt cattcctcat ggagacatcc acaaactgga cttatgatat caactatttt
     1861 aagtgcatgt gaagattcgc ctattgtcaa attacgacac tcagttagtg aataaaatca
     1921 gtctatatct tacatattaa tggttaaaaa agcgataatt gcttgatgtt cttgattaac
     1981 aagtgattaa tgaaatttat acttggtgct agaatgaaaa tcatttgata cttttgcgcc
     2041 gattttagta caaacatata attggcttcg aatgaataag ttcacaaaag caggactgct
     2101 gtttattatt ccgataacac ttctgggcgt tgccagttgt ggaattaaga aaatggttaa
     2161 agatgcgggt aaaattagct atgaggctaa acctaatccg cttgaattac atggagacag
     2221 tgttaaagtt tctgtaacag gaaattttcc agctaagtac ttccataaga aagcaacttt
     2281 aactgttacg cctgtcatta agtatacagg aggagagaaa gaacttaaga gtattattct
     2341 tcgtggagat aaggtagaag gtgcaggaca gaatattact tatacagaag gagggaagtt
     2401 ctctgtatct gatcagtttt tatatgtaac aggtatggat aaatgcgaag taattcttcg
     2461 ttcaaccgcc cagtacaaat caaaaacaac cacattacct gaagtttcga tagcaaaagg
     2521 tactatcgtt acaccattga tgttgaagaa agattatcaa gcaagtgttg ctgtagataa
     2581 attcgataag aatcctactg ttactcagaa agctaacatt tactatttgg tagattcttg
     2641 ggaagttaga aatgtggagc ttaagagtga tgagtcggac aatatgaacc gttttgtaga
     2701 acgtgctgcg aaggatggtt ctgaattcac caaattggat atttacggat atgcttctcc
     2761 agaaggggaa ctttcacgta actctaagct gtccgaaaat cgtgctgatg aagcttacaa
     2821 agtaatgtta gcgaaattca aaaaagctaa gttgaaagac ctagagaaga aagaaggttt
     2881 ctattctaaa atcactactg attatgaaga ttgggatgga ttgaagaaaa tgcttcaaac
     2941 ttcatctatt gccggaaaag acgaagcaat gaaagttatc acaagcatta ctgatccaga
     3001 agctcgtgaa gaagaattta agaaaatggc ttcatttgac cctatttacg aagcttattt
     3061 ccctaaactt cgtcgtgctg aaatcaattt ggtggctaaa atcaaaacac gtacggatga
     3121 tcagattcgt gttgctgcca ttgcttcacc tgattcatta ggtatggaag aattgcttta
     3181 tggttctact ttgttggatt cggatgatga taaattgaaa gtctatcaat catatgctcg
     3241 tcgtttccct gaagatttcc gtggatttaa caatactgga gttatttacg taaaacaagg
     3301 taaaatcaac gaagctcaac aagaatttga aaaagcatcg aaagttgcgg ggaataatgc
     3361 acaggttcaa aataacctag gtgttgtagc tgcaatgaaa ggtgatcgta aagcagcagc
     3421 gcagtttttt gcagctgctg gaacaactgc tgaagcatta cataataaag gtaatcttgc
     3481 tgtagcaagt ggaaaatatg ctgatgcaac tagtaactat ggttctgagt gcagctttaa
     3541 tgcagcatta gcgaaattac ttgctggtaa tgcttcaggt gcaactcaaa cattagattg
     3601 cagtgatgat aaagatagcg ctgaaggttt ctatctacgt gctgttattg ctgctagaag
     3661 taataataag gatggagttg tttccaattt aacaaaagct atctcagcta agtcaatctt
     3721 gaaggaa
//
LOCUS       DAOOQW010000233         3723 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_29503_length_3723_cov_4.259815, whole genome shotgun sequence.
ACCESSION   DAOOQW010000233 DAOOQW010000000
VERSION     DAOOQW010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3723
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29503_length_3723_cov_4.259815"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1945
                     /gene="dnaE"
                     /locus_tag="PK281_10515"
     CDS             <1..1945
                     /gene="dnaE"
                     /locus_tag="PK281_10515"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOY49818.1"
                     /translation="VHAAGIIIAPEDLTNLIPVAVAKDSDLLVTQYEGSVIEDAGVIK
                     MDFLGLKTLTIIRDALQLIKENHGISIDIDSIPLDDVKTYELYQKGETNGTFQFESDG
                     MKKHLRDLKPDKFEDLIAMNALYRPGPIEYIPNYIKRKHGTEAVSYDVPEMEEYLKET
                     YGITVYQEQVMLLSQKLAGFTKGMADKLRKAMGKKDRKTLDELKPKFISGANEKGLDP
                     SKLEKIWTDWEAFAAYAFNKSHSTCYAFVAFQTAYLKAHYPAEFMAANLTNNLSSMDK
                     ITFFMEECKRMGLKVLGPDVNESKLKFSVNQKGQIRFGLAAVKGVGEAAVESLITERA
                     ANGHFASIFDFVKRVSLRAVNKKSIEGMAIAGAFDEFIQSNRSVFFHSEMPGKPTFLD
                     TIVKYGQSVQEGKEHAATSLFGDSSDEISIPEPIIPKAEPWHVLEMLEKEKEVVGIYL
                     SGHPLDDYRQEMKNFNFIPMAELENLEQFRSKSECKSGGIITSVGHRFTKTGKPFGSF
                     VIEDFSGSREFVLFSEKYLKLKHYLDPGNIIYINWKPNKSFRNPDGLETDILTMGVLA
                     DLKDKYTQITMQVNETEMSEVFVNALVDIFLRHPGKCSIKMILAANDATLSVQTKANF
                     KVDTNPDFLKELETLIGDRYRLN"
     gene            1999..2337
                     /locus_tag="PK281_10520"
     CDS             1999..2337
                     /locus_tag="PK281_10520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49819.1"
                     /translation="MRSFLFLGFSVIVLSCFTQQVSAQSLPTPVRFQTLQGKVYQADG
                     IQGSSIKFDSIKPFLFDITATNPNPPKELYRIHEDKLFLGKNGRFITFSTSRDTLYFQ
                     PSGTRYIKVN"
     gene            complement(2334..3041)
                     /locus_tag="PK281_10525"
     CDS             complement(2334..3041)
                     /locus_tag="PK281_10525"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HOY49820.1"
                     /translation="MTTSEVLKELKAMGSEQIRAIFINHGAPADQLYGVKVGDMKKFL
                     KPLKGNQATALELYDTGVSDAMYLAALVADGKKMTREQLQHWVETATWNMISEYSVPW
                     VASESPFALELGLKWIDDSKEHIAAAGWTTLCCWLSIVPDEEIDRSLMLNLLNRIEKE
                     IPQSANRVRYCMNTFIIGLGGYVRDLSNEAILTAKRVGQVQVYQGNTNCKVPDAASYI
                     QKMIDRGPVKKKKTAKC"
     gene            3120..>3723
                     /locus_tag="PK281_10530"
     CDS             3120..>3723
                     /locus_tag="PK281_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOY49821.1"
                     /translation="MITSEKIHSIFPGFEKDLIEQLIQVAQEKSVNSGEYLMKTGQHF
                     RNVMVVIEGTIKVYREDDEGNEFFMYYLESGQACALSMICAARFQASQLMAKAETDVT
                     LLTIPLQYMDEWMSKYKTWYYFVLENYRSRFDEVLRTLDHVAFRNMDERLVYYLKRYQ
                     DTHKTNLVKISITEIAQELNSSREVISRLLKKLNEKEIGDY"
BASE COUNT         1203 a          691 c          694 g         1135 t
ORIGIN      
        1 tgttcatgct gctggaatca tcattgcacc agaagacctt actaacctca ttcctgttgc
       61 agttgcgaaa gattccgatt tattggtaac tcagtacgaa ggttctgtaa tcgaagatgc
      121 aggtgttatc aagatggact ttttgggact gaaaaccctc accatcattc gtgatgcact
      181 tcaattaatc aaagagaatc acggaattag tatcgacatc gattccatac cgctcgatga
      241 tgtgaaaaca tacgaattgt accaaaaggg tgaaacaaat ggaacattcc agtttgaatc
      301 ggatggaatg aaaaagcatt tacgtgattt gaaacccgat aaattcgagg atttgatcgc
      361 gatgaatgct ttgtatcgac caggaccaat cgagtacatc cccaattata tcaagcgtaa
      421 acacggaact gaagctgtta gttatgacgt tcccgaaatg gaagaatacc tgaaagaaac
      481 atacgggatt accgtatatc aggaacaggt aatgttactt tctcaaaagc tagctggttt
      541 tactaaaggt atggccgata agctgcgtaa agcaatggga aagaaagatc gtaaaacgct
      601 ggatgaatta aaacctaaat tcatttcagg tgccaatgag aaaggactag atccgagtaa
      661 attggagaaa atctggaccg actgggaagc atttgctgcg tatgcattca ataaatcaca
      721 ctccacttgt tacgcatttg tagcctttca aactgcctat cttaaagccc actaccctgc
      781 cgagtttatg gcagccaact taactaacaa cctaagttct atggataaaa ttacattctt
      841 tatggaagag tgtaagcgca tgggattaaa ggttttaggt ccagatgtga atgaatcaaa
      901 attaaaattc tccgtaaatc aaaaagggca aattcgattt ggtttagctg ccgttaaagg
      961 cgtgggtgaa gctgctgtag aaagcctaat tacagagcga gctgcaaatg ggcattttgc
     1021 tagcattttc gattttgtta agcgagtaag tttacgtgct gtaaataaaa agtcaattga
     1081 aggaatggca attgccggag catttgatga atttatccag agtaatcgtt ctgtgttttt
     1141 tcattccgaa atgcccggaa aaccaacatt tctcgacaca atagtaaaat atggacaaag
     1201 tgttcaagaa ggaaaagagc acgctgcaac tagtttattt ggtgattcat cggatgaaat
     1261 ttcaattccc gaaccaatca ttcctaaagc agaaccatgg catgtgctag aaatgcttga
     1321 gaaagaaaaa gaagttgtag gtatttattt aagtggacat ccacttgatg attatcgaca
     1381 agaaatgaaa aatttcaatt tcattcctat ggctgaattg gaaaatctgg aacaatttcg
     1441 cagtaaatcg gaatgtaaaa gtggagggat tatcacatcc gttggacatc gctttaccaa
     1501 gacaggaaag cctttcggaa gttttgtgat tgaagatttc tcgggctcta gagagtttgt
     1561 tttattctca gaaaaatacc tcaaactcaa acattacctc gatccaggaa atatcattta
     1621 tatcaactgg aaacccaata aaagtttcag aaatccagat ggtctagaaa ccgatatcct
     1681 cactatgggt gtgcttgcag atttaaaaga caaatacact caaattacta tgcaggtaaa
     1741 tgaaaccgag atgagtgagg tattcgtaaa tgcattagta gacatttttc ttcgtcaccc
     1801 agggaaatgt agcattaaga tgattcttgc ggctaacgac gctactcttt cggttcaaac
     1861 gaaagctaat tttaaggtag ataccaatcc tgatttcctc aaagaattgg aaactctcat
     1921 tggtgatcga taccgattaa attaaaattt atttggtatt cagatttttt cctactttta
     1981 ttcaaataac aaacagctat gcgatctttc cttttcctag gtttttcagt aattgttctt
     2041 tcttgcttta ctcaacaggt gtctgctcaa tctttgccaa ctcctgttag atttcaaaca
     2101 ttgcaaggga aagtatatca agctgatggt attcagggtt cttcaatcaa atttgattca
     2161 attaaacctt tcctattcga cataacagct actaatccta atcctcctaa agaattatat
     2221 cgtattcatg aggacaaatt atttcttggg aaaaatggta ggtttataac atttagtact
     2281 tcaagagata cactttattt ccaaccttca ggaacacgtt acattaaagt caattagcac
     2341 ttagctgttt tcttcttttt aaccggtcct cgatcaatca ttttttgaat gtacgaagca
     2401 gcatcaggta ctttacagtt tgtattccct tgataaacct gcacctgccc tactcgcttt
     2461 gcagttaata tagcttcatt acttaaatcg cgcacgtaac ctccaaggcc gattataaac
     2521 gtattcatgc aataacgtac tcgattagcc gattgaggaa tttctttctc gatccgatta
     2581 agtaaattca acattaaact acggtcgatt tcttcatctg gcacaataga caaccaacaa
     2641 cacagtgttg tccagccagc agcagcaata tgctcttttg aatcatcaat ccatttcaat
     2701 cctaattcta acgcaaatgg gctttcggaa gctacccaag gcaccgagta ttcacttatc
     2761 atattccatg ttgcggtctc aacccaatgt tgcaattgct ctcgagtcat tttcttacca
     2821 tctgcaacta aagcagctaa atacatcgca tccgaaacac ctgtatcata gagttctaat
     2881 gcagttgctt gattaccttt taatggcttt agaaacttct tcatatcacc tactttaact
     2941 ccgtaaagct gatctgcagg cgctccatga tttatgaaaa ttgctctgat ttgctctgaa
     3001 cccattgctt tgagctcttt caacacttct gatgtagtca tacctagata ttatcaagcg
     3061 aatatactgg ttgatacaac taggtaaaat aaattggaaa tttttattga atttgcggca
     3121 tgattacttc agaaaaaatc cattcaattt tccccggttt tgaaaaagat ctcattgaac
     3181 aattaattca agttgctcaa gaaaaaagtg tgaattcagg ggaatatttg atgaaaactg
     3241 gccagcattt ccgaaatgtt atggttgtaa ttgaaggtac aattaaagtg tatcgggaag
     3301 atgatgaggg aaatgagttc tttatgtact atttagaaag tggacaagct tgtgctttat
     3361 ctatgatttg tgccgctcgt tttcaagcta gtcaactaat ggcgaaagcc gaaacagatg
     3421 ttactcttct tacaattcca ttgcaataca tggatgaatg gatgagtaag tataaaactt
     3481 ggtactattt cgtattggaa aattatcgtt ctcgttttga tgaagttctt agaacacttg
     3541 atcatgtagc atttaggaat atggatgaac gattagtgta ttacctaaag cgctatcaag
     3601 acactcataa aacaaatctg gtcaaaatat ccattaccga gatagctcaa gaattaaatt
     3661 catctcggga agttatttct agattactta aaaagctgaa tgaaaaagag attggtgact
     3721 ata
//
LOCUS       DAOOQW010000234         3686 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_29893_length_3686_cov_4.643624, whole genome shotgun sequence.
ACCESSION   DAOOQW010000234 DAOOQW010000000
VERSION     DAOOQW010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3686
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29893_length_3686_cov_4.643624"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..848
                     /locus_tag="PK281_10535"
     CDS             <1..848
                     /locus_tag="PK281_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019386673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HOY49822.1"
                     /translation="AINKHTARGTYFFDYGNAFLLEASRAGADVMANDGINFKYPSYV
                     QDIMGPMCFDFGFGPFRWVCTSGKDADLDFTDHVAKDVLEEILKHAPQEIQSQLNDNI
                     QWIKQAKSNKLVVGSKARILYANAEGRMKIAQAFNQAVREEKIGPIVLGRDHHDVSGT
                     DSPYRETSNIYDGSRFTADMAIHNVIGDSFRGATWVSIHNGGGVGWGEVINGGFGMLL
                     DGSEDADRKLKNMLHFDVNNGIARRAWARNDIAVSAIKREMELTKDLLVTLPNPVSDS
                     ILDRL"
     gene            1037..1630
                     /locus_tag="PK281_10540"
     CDS             1037..1630
                     /locus_tag="PK281_10540"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HOY49823.1"
                     /translation="MNPFKKVMLIALLTAIGSAAFAQAQFSVGLKGGLNFANLDVKSI
                     SNTYDNRTGYHAGAFALVKFTKIGIQPEVIFSQQGSTVKVNGDDIQSNFSYVNIPIMV
                     KLYTVAGINLQVGPQFGFLTNDPVVVNDNEEIEGAVKKSDISLGLGVGWDLPFGLSID
                     GRYNLGLQNVSDNSAFDIKNQVFQVSVGFKLIKLGGK"
     gene            complement(1710..2417)
                     /locus_tag="PK281_10545"
     CDS             complement(1710..2417)
                     /locus_tag="PK281_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008992878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOY49824.1"
                     /translation="MFSTDPVVRVKEASIFQDNNTVLGDISFDIEKGEFVYLVGRTGS
                     GKSSLLKTLYADLALRLGDIQVSGFNIRGIKNSEVPLLRRKLGIIFQDFQLFPDRSVD
                     ENMMFVMRATGWTDNGKMRARLSDVLMQVGLGSVEKKMPHQLSGGEQQRVVIARALLN
                     EPQILLADEPTGNLDPEVSHGIMKIFQQINKSGTAVLMATHSYGLIKKFPYRVLKCEE
                     GKVIDSAKEQFELVTDY"
     gene            complement(2518..3174)
                     /gene="fsa"
                     /locus_tag="PK281_10550"
     CDS             complement(2518..3174)
                     /gene="fsa"
                     /locus_tag="PK281_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013307258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HOY49825.1"
                     /translation="MKFFIDTGNLNEIKEAYDLGVLDGVTTNPSLMAKEGIKGAENIK
                     AHYKAICNIVDNNVSAEVIATDFDGMIKEGYELAKIDDKIVVKIPMIKDGVKALKKFS
                     SEGIRTNCTLIFSAGQALLAAKAGASYMSPFIGRLDDIGLEGMDLIAQIRLIYDNYGF
                     DTEILAASVRNTVHLLQCAEIGADVATCPLNVITGLLNHPLTDSGLAKFLADYKKVNG
                     "
     gene            3365..>3686
                     /locus_tag="PK281_10555"
     CDS             3365..>3686
                     /locus_tag="PK281_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008634514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HOY49826.1"
                     /translation="MQCGIVGLPNVGKSTLFNSISNNKAEAANFPFCTIEPNVGVITV
                     PDPRLKILEALVNPQRVLPTTIEFVDIAGLVKGASKGEGLGNQFLANIREVNAIVHVI
                     RCFDN"
BASE COUNT         1104 a          688 c          774 g         1120 t
ORIGIN      
        1 cagcgatcaa taaacacaca gcacgcggta cttatttctt tgactatgga aatgcttttt
       61 tactggaagc ttcgcgtgca ggtgcagatg taatggcaaa tgatggaatt aattttaagt
      121 atccatcata cgttcaggat atcatggggc ctatgtgttt tgattttggc tttggtccgt
      181 ttcgttgggt gtgtacctct ggtaaggatg ctgatttaga ttttacggat catgttgcca
      241 aagatgtgtt ggaagaaatt ttaaaacacg cgccacaaga aattcaatca cagttgaatg
      301 acaacattca atggatcaag caagccaaga gcaacaaact ggttgtggga tcgaaagcaa
      361 gaatattgta tgcgaatgcg gaaggcagaa tgaaaattgc tcaggcattt aatcaagcgg
      421 ttcgtgaaga aaagattgga ccaatcgtgt tgggaagaga tcatcatgat gtttccggaa
      481 cggatagtcc gtatagagaa acatccaata tttatgatgg atcaagattc actgcagata
      541 tggccattca taatgttatt ggtgattcgt tccgcggagc aacctgggtt tccattcaca
      601 atggtggagg tgtaggatgg ggagaggtaa tcaatggtgg ttttggtatg ttgttagatg
      661 gaagcgaaga cgctgaccgt aaactaaaaa atatgcttca ctttgatgta aacaacggca
      721 ttgcacgaag agcctgggcc cgaaatgata ttgcagtatc cgcgattaag agagaaatgg
      781 agctaacaaa agacctgctg gttacgttac ctaatccggt tagtgattca atcctggatc
      841 gattgtagaa atcgttaatg gttatcggtt aatcgtggga gagagattgg gatgtgatga
      901 tattgtgtca tttattggac acttcttgag gattgaacca ttaacaatta acgaataacg
      961 attaactgat aacagggtac taaaaaaaat tcggcttcgt ttttgattag aaaatatcca
     1021 aacaaaactc aaaattatga atccatttaa aaaagttatg ttgattgccc tgcttaccgc
     1081 gattggttca gcagcatttg cgcaagcaca attctcggtt ggtcttaagg gtggtcttaa
     1141 cttcgcgaac cttgatgtta aaagcattag caatacctac gacaaccgaa caggatacca
     1201 cgctggggct tttgcgttgg ttaaattcac gaagatcggc attcaaccgg aagtcatttt
     1261 ttcgcagcaa ggatctactg ttaaagtaaa tggcgatgat attcaatcta actttagtta
     1321 tgtgaatatt ccaattatgg taaagctgta taccgttgcc ggaattaatt tacaggtggg
     1381 tccacagttt ggttttttaa ccaatgatcc tgttgttgta aacgataatg aagagattga
     1441 aggggctgta aaaaagtctg atataagtct cggcttagga gtaggttggg atttaccttt
     1501 cggtcttagc attgatggtc gatataatct tggtcttcaa aacgtaagtg ataattctgc
     1561 tttcgatatt aagaatcagg tatttcaggt ttcagttgga tttaaactga tcaagctggg
     1621 aggtaaataa aggtgtaatg taaaaggaaa gaggtacgaa gtgagtaaat ccacttcgta
     1681 cctctttcct ttttttacct tcgtacgtct taataatctg taacgagttc gaattgttct
     1741 tttgctgaat caatcacttt accttcttca cattttaata cgcgatacgg aaatttctta
     1801 ataagaccat aactgtgtgt cgccatgaga actgcggttc cacttttatt aatctgttga
     1861 aaaattttca taatgccatg cgatacttcc ggatccagat tcccggtagg ttcatcggcc
     1921 aataaaatct gaggttcgtt taataatgct cgcgcaatca caacacgctg ttgttcccct
     1981 ccagaaagtt gatgcggcat tttcttttct accgaaccta aaccaacctg cattaataca
     2041 tcgcttaaac gagcacgcat ttttccatta tcagtccagc cggtagcacg cattacgaac
     2101 atcatgtttt catctaccga tcgatcgggg aaaagttgaa agtcctgaaa gatgatacca
     2161 agcttcctgc gcaataaagg aacttctgaa tttttaattc cacgaatgtt aaatcccgaa
     2221 acttgaatat cgccaaggcg caaagctaaa tcagcataaa gcgttttcag caaagaagat
     2281 ttaccagagc ccgttcgtcc aacaaggtaa acgaattcac ctttttcaat atcaaaagag
     2341 atatccccca gaacggtatt gttgtcttga aaaatactag cctcttttac gcgtacgacc
     2401 ggatcagtag aaaacatgtt taaagtgttt tggtgctttt gtgtttaggt gtttaagttc
     2461 gtaaccatat tttcaatatt taaaaacttc aaacttgaaa catcaaacta tgaacgctta
     2521 accattcacc ttcttataat ctgctaagaa ttttgcaaga ccactatcag tcaatggatg
     2581 gttcaacaaa cctgtgatta cattcaaagg gcatgttgca acatcagcac cgatttcggc
     2641 acattgaagt agatgaactg tgttacgaac tgaggcagca agaatttcgg tatcaaatcc
     2701 gtagttatca tagatcagtc tgatttgagc gatgaggtcc ataccttcca atccaatatc
     2761 atctaagcga ccaatgaatg gcgacatgta acttgctcca gcctttgcag ctaataatgc
     2821 ctgacctgca gagaatatca atgtacaatt ggtacgaata ccttcgctgc tgaatttttt
     2881 caaagcctta acgccatcct taatcattgg aattttcacg acaatcttat catcaatttt
     2941 agcaagctcg tagccttctt tgatcatgcc atcaaaatcg gttgcgatca cttccgcact
     3001 cacgttattg tcaacaatgt tacaaatggc cttataatga gctttaatat tttcggcacc
     3061 tttaatacct tcttttgcca tcagagatgg gttggtggtt acgccatcca atactcccag
     3121 gtcgtaagct tctttgattt cattcagatt gccggtgtcg ataaagaatt tcattgattt
     3181 atgctgtttg gttttaaatt tcgagggcga agttaagcat catttataga agtgcataga
     3241 aattaagttg ccagtttcag gttgccagtt cccggtaacc ggaaactggc aacccgtatt
     3301 agtgtcaatc caaaaactgc cagtatattt gcgcccttgt tctaaaatta ttttaactat
     3361 ggcattgcag tgtggaattg ttggactacc caacgttgga aaatcaaccc tttttaattc
     3421 gatttcaaat aataaggctg aagcggccaa cttccctttc tgtacaatag agccaaatgt
     3481 tggtgttatt acagttccgg atccaaggtt gaaaatatta gaggcattgg taaatcccca
     3541 aagagtattg ccgaccacga ttgagtttgt tgacatcgct ggtttagtaa agggtgcaag
     3601 caagggtgaa ggattgggaa accagttttt agcaaacatc cgggaggtaa atgccattgt
     3661 gcacgtaatt cgatgttttg ataacg
//
LOCUS       DAOOQW010000235         3670 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_30045_length_3670_cov_4.913693, whole genome shotgun sequence.
ACCESSION   DAOOQW010000235 DAOOQW010000000
VERSION     DAOOQW010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3670
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30045_length_3670_cov_4.913693"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="PK281_10560"
     CDS             complement(<1..351)
                     /locus_tag="PK281_10560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49827.1"
                     /translation="MPAGFSRYILISFVLSLLCASFAGLAASKGILVLSPTFRYGPLL
                     FFFLLSCALHYYLLRSTEGRPQRFVTAFMGVQALKMFVHLLVMTIVGFVFPAIAVHFI
                     ILYAVYYLSFTVVET"
     gene            complement(535..960)
                     /locus_tag="PK281_10565"
     CDS             complement(535..960)
                     /locus_tag="PK281_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010231525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOY49828.1"
                     /translation="MFNSKDSKSSDSMSVSGTNQILSGTMITGDIICDGNIRIDGTLK
                     GNLQAKGKVVIGPSGLIDGELNCQNADISGTVKAKVTVAEQLTLKATAKLSGDISTNK
                     LAIEPGANFTGACSMGGIVKDFTKHGAQNNKQVLQEKTA"
     gene            complement(1032..>3670)
                     /locus_tag="PK281_10570"
     CDS             complement(1032..>3670)
                     /locus_tag="PK281_10570"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOY49829.1"
                     /translation="YIFHLQKLNYIRPLLYALLVLLITGGLIGCSTKKNKWINRTFHN
                     ITSKYNGYFNGEETIKEAVATLRTGHVDNYIKVLPVYDLGSLEDSKTIIPLTDKAIKK
                     ASVVISRHSMMIKGKEYCKYIDDSYLLIGKSMFYKRDYYASLEMFAYVAREPVKNNKK
                     DPIQHRANLWLCRAYSELGMFTDARLAIDRSLNDKTLPKNIKGELYTALTDFHLKQNH
                     YAEGLETITEALKYVKKKKDRVRLLFIRGQLLQKAGEIKQASDSYAKVLKSNPTYEMS
                     FYARINMARCFDSENKKSTEIRALLAKMLKDPKNLDYKDQIYYVLGELDEKEGNETKA
                     IEQYTTSVQASTTNVNQKGMSYLAIAEIYFDQREYRISAAYYDSTISSLQKDFPDYKI
                     IENKRNSLAELVKMYEKIELYDSLLRMAKLNPAELDKKAEQIFKKEEEARRKQKEKDA
                     ILAAKLAASGGGTSAAPGIAGNGSWYFYNTSTMSFGYTEFRKVWGDRKLEDNWRRSNK
                     QTVAPISGNPDEDTTGSDVAKEEIKLTREDSIALIRKKLEKAVPKNDTMLRAYADSVM
                     EGYYDLGLIYKERLADLRKAAETYEDFLKRYPNNSNEPTIFYQLYRIYLAIPDNPKAE
                     DYKKRLINKYPNSEYTQIITDPEFFKRESLSKSEAVKFYEETYRLYQARQYAEVINRC
                     RTSETRYQGNALAPKFALIKALAIGQTRDVDAFKSSLQFVVKNYPTDSVKTKAAELLN
                     SLSKFQGTGTKDSVAAPTFSYKADTLHYYVVMVESVTLNLNDVQTRLSDLNTLYYSLK
                     DLKINPRLLGKNYQIVSVQVFLNRKEASDYMKMIDDDDLIFQEIDPNILDTFIISAAN
                     FNTLMKNSKVEEYLDFYKRVYQ"
BASE COUNT         1035 a          734 c          713 g         1188 t
ORIGIN      
        1 tgtttctacc accgtaaatg acaagtaata aacggcatac aagattataa agtgtaccgc
       61 aatggcagga aaaacaaatc caactatcgt cattacgagc aggtgaacaa acattttcaa
      121 ggcctgcaca cccataaaag cagtaacgaa acgttgcggt cgtccttcgg ttgagcgcag
      181 aagataataa tgaagggcac aacttagcag gaagaagaaa agcagaggtc cgtatcggaa
      241 agtaggacta agaaccaaga ttcctttgga agcggccaat ccggcgaaac tagcacagag
      301 cagactaagt acaaatgaaa tgaggatata acgtgaaaat ccagcaggca tcagcgtttg
      361 ggtaaggaag tgaacaacca gaacatacta gcagaaatgc ccaaaatagc tccgataaga
      421 gttagaactg gaaaccgaaa atcgaacttg ttatcagccc agtctccgag ccaagtaagc
      481 acaagaatag cacccatcat ttgaatgcct aatccggcgt aacgaccgta atcgttaagc
      541 tgttttttcc tggaggactt gtttgttgtt ttgggctcca tgcttggtga aatcttttac
      601 gataccaccc atgctgcaag caccagtaaa gttggctcct ggttcaattg ctaatttgtt
      661 ggtgctgata tcaccgctca acttagcagt tgctttgagt gtaagctgct cggctactgt
      721 aacttttgct tttacagttc ccgaaatgtc ggcattctga caattcaatt ctccatcgat
      781 caatccgcta ggtccgatta ccactttccc tttagcttga agatttcctt tcaaagtacc
      841 gtcaatgcgg atatttccat cacagataat atcgccggta atcatcgtgc cggacaaaat
      901 ctggtttgta ccggaaacgc tcatggagtc agatgactta gaatctttgg agttgaacat
      961 cttggaggtg tattttttcc tttggggcct caaagataga aagaaatttc cgctacgaaa
     1021 atgaagagtg tttattggta tacccgtttg taaaagtcga ggtattcttc cactttcgaa
     1081 tttttcatca aggtgttgaa attagctgca ctgatgataa acgtgtcgag tatattcgga
     1141 tcaatttctt ggaaaataag atcatcatca tcaatcatct tcatgtagtc gcttgcttct
     1201 ttccgattaa gaaaaacttg aaccgaaact atctgatagt ttttacccaa taatcttgga
     1261 ttaattttca agtcttttag cgagtaataa agcgtattca aatcacttaa acgggtttga
     1321 acatcgttga gattaagtgt tacactttca accattacaa cataatagtg caatgtgtcg
     1381 gctttgtatg agaatgttgg agcagcaaca ctgtctttgg tgcctgtacc ttgaaattta
     1441 cttaggctat tgagtaattc ggctgctttt gttttaaccg aatcggttgg atagtttttt
     1501 acaacaaatt gtaagctaga cttaaatgca tctacatcgc gcgtttgtcc aattgctagt
     1561 gctttaatta aagcaaattt tggagctagt gcatttcctt ggtaacgagt ttcggaggta
     1621 cggcaacgat tgattacttc ggcatattgt cgagcttggt aaagtcgata tgtttcttca
     1681 tagaacttta ctgcctcaga tttacttaaa ctttcgcgtt tgaaaaactc tggatcggta
     1741 atgatttgag tgtattctga gttaggatac ttgttaatca aacgtttttt atagtcttca
     1801 gctttaggat tatctggaat tgccaaataa attcggtaga gttgatagaa aatggttggc
     1861 tcattcgagt tattcggata acgttttaaa aaatcctcat atgtttcggc tgcttttctc
     1921 aaatcagcta atcgctcttt gtaaatcaat ccaagatcgt aatatccttc catcaccgaa
     1981 tcagcatacg cgcgcaacat ggtgtcgttt ttaggaacgg ctttttctaa ttttttacgg
     2041 attagtgcta tgctatcttc acgagttagc ttaatttctt ctttagcaac atcagatcct
     2101 gttgtgtctt catcgggatt gcccgaaata ggagcaacag tctgtttatt ggaacgtctc
     2161 cagttatctt ctaatttccg atcgccccaa actttacgga attcagtata accaaagctc
     2221 atcgtggaag tattatagaa ataccaactt ccattacctg caattcctgg tgctgctgaa
     2281 gttccaccac ctgatgccgc taattttgct gctagaattg catctttctc tttttgcttt
     2341 ctgcgtgctt cttcttcttt cttgaaaatc tgctcagcct ttttatccaa ttcagccgga
     2401 ttcaattttg ccatccgaag taaactatcg tagagctcaa tcttctcgta cattttaacc
     2461 aattcggcta agctgttacg cttgttttcg atgattttat aatctggaaa atctttttgt
     2521 aaggagctga ttgtactatc ataataagca gccgaaattc ggtactcgcg ttgatcaaaa
     2581 taaatctcag ctatagccaa ataggacatt cccttttgat tcacattcgt cgtacttgcc
     2641 tgaacagatg tggtgtattg ctcgattgct ttggtttcgt tgccctcttt ttcatccaac
     2701 tcacctaaca cataatagat ctgatctttg taatctaaat tcttcggatc cttaagcatc
     2761 tttgctaata aagctcggat ttcagtgctt ttcttattct cagaatcgaa gcagcgagcc
     2821 atattgattc gagcataaaa cgacatctca tacgttggat tgcttttcaa aaccttagca
     2881 tagctatcgg aagcttgttt tatttctcct gctttttgta gcaattgtcc gcgaataaag
     2941 agcagtctaa cccgatcttt tttctttttc acgtacttca aggcctcagt aatcgtctcc
     3001 aatccttcgg cataatgatt ttgcttcaga tggaaatcgg tcaatgcggt atacaattct
     3061 cctttaatgt ttttaggaag agttttgtcg ttgagcgatc tatcgattgc taagcgtgca
     3121 tctgtaaaca tacccaattc gctgtaggcg cgacaaagcc aaaggttggc tcggtgttga
     3181 atgggatctt ttttgttgtt tttaaccggt tcgcgtgcta cataagcaaa catctctaat
     3241 gatgcatagt agtcgcgttt gtaaaacatc gatttcccga ttagtaaata actatcgtcg
     3301 atgtatttac agtactcttt gcctttaatc atcatcgagt ggcggctaat aaccacagat
     3361 gctttcttaa tagctttatc ggttaaggga atgatggttt tggaatcttc taaagatcca
     3421 agatcataca caggaagaac cttaatgtaa ttatctacat gccctgttcg aagtgtagca
     3481 acggcctctt taatagtttc ttcaccatta aaatatccat tgtatttgga ggtgatatta
     3541 tgaaatgtcc gattaatcca cttgtttttc ttagtggaac acccgattaa tccacctgtt
     3601 ataaggagaa ctaaaagagc ataaagcaga gggcgtatgt agttgagctt ttgcaaatga
     3661 aaaatatacg
//
LOCUS       DAOOQW010000236         3665 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_30104_length_3665_cov_7.470637, whole genome shotgun sequence.
ACCESSION   DAOOQW010000236 DAOOQW010000000
VERSION     DAOOQW010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3665
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30104_length_3665_cov_7.470637"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="PK281_10575"
     CDS             complement(<1..221)
                     /locus_tag="PK281_10575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49830.1"
                     /translation="MYGQFISNGLPETRVQVDIEPGVLRFQNGAQSKELRSQDLIEVS
                     YPGKNKALLLFREGSLFLEAPNIQGTLRH"
     gene            complement(226..1158)
                     /locus_tag="PK281_10580"
     CDS             complement(226..1158)
                     /locus_tag="PK281_10580"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HOY49831.1"
                     /translation="MITIFKTANRRLEEIQKPEPGCWVNVVDPSDEELAWLIQEFPWA
                     EEYHLDPLDIDEKARAERDEDVLFTLIKIPHFQGKKDEAPFVTIPMGVIQNNDYFITI
                     TKEDNVIVNEFENNRVKNLQTQKHYRFLLLILLKTATKFLAHLREINKTVDQIEDDLQ
                     LSMRNQDIFDLLKYQKVLIYYTTALKSNELMMARLNRLQLLRTYEEDEELFQDVVTEF
                     QQAIEMTNIANNILLQMTSAYASIINNNMNKIIKTLTLITIVLYVPTLISSFFGMNVI
                     IPGQTSSSAFAWILGISLTLSATILFIFMRRRWF"
     gene            1383..>3665
                     /locus_tag="PK281_10585"
     CDS             1383..>3665
                     /locus_tag="PK281_10585"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HOY49832.1"
                     /translation="MNLRFLFILLFISSSVLSQTFVEGMVRDSAQKRPLAFVSIAVQG
                     FPEATAMSDIDGFFRILLPNGYPAQLNFSYIGYRSEQLTLESANGKSKLRVELTPSSL
                     QLRTFEFLAGENPAHRIIRETWKRRDQHDPEKLKSYRCQTYNKLVLSGRPDTAFDAKK
                     IEDQQKLKSYDSLFSKQHLFMIESSNERLFRNGKVKENVIGSKVSGLQEASVFMLALQ
                     FQPFSFYAPLVELNGQKLLNPISRNSEDVYSFVLEDTLFSGRDSVYVIRFQPRKNSTI
                     EGLKGVLYISAPDYAIQNVVAEPASDNSTLRLRVQQQYKKTETGVWFPEQLNTDMIFL
                     TVKLTGYKMYGESRTYVSKPEINPELSAKLFDEAELDVQKGSSEKSQAFWQTVRIEDL
                     TEQEQQTYYQLDSLFRAENMAPKIKAVEALVRGYIPIKWFDLELNRIMRFNNHEGFRL
                     GAGGITNDKVSRHFGIGGYIAYGFKDQVTKYGAESRIYLYPRRELELRMMYQQDVYES
                     GFQTFVNDRKPYLTEQVRQVIVRNMNGEERSEVSLGWRWLKFLRTDLFIRQAHVKTLF
                     DYQYDSLASNTRYTWLEQGASFTYCYKEKFYRNGKLKISLGGKTPVFRLMLTRGSDLS
                     LGGKTDYLRADFRMEYAYQWRRIGKTVMLLSAGMIDGNVPYARLYNGKGNLSGNADLR
                     AVSTNSFETMHMNEILTDRFASLFFQHHLGSFFHVGTYKPLFILVHNMMVGTLGNTNR
                     SAQKIISFTVPEKGYFESGLL"
BASE COUNT         1063 a          801 c          755 g         1046 t
ORIGIN      
        1 aaatggcgca gcgtaccttg aatattaggt gcttccaaaa acagacttcc ttcccgaaat
       61 aacagcaatg ctttgttctt accgggatag cttacttcaa tcaaatcttg ggaacgtaat
      121 tccttgctct gagctccatt ttgaaaacga agcacaccgg gttcaatatc aacctgaacg
      181 cgggtttcgg gtaatccgtt ggagataaac tgaccgtaca ttgttttaga accagcgtct
      241 acgcatgaaa atgaacaaaa tagtcgcact caaggtgagc gaaatgccca aaatccaagc
      301 aaatgctgag ctggatgttt gtccgggaat aatcacattc attccaaaaa agctcgaaat
      361 caaagtcggt acgtacaaca caatcgtgat taacgtaagc gttttgataa tcttgttcat
      421 gttgttattg atgatactgg cgtaggccga agtcatctgc aacaagatgt tattagcgat
      481 gttcgtcatc tcaatagcct gctggaactc agtcacaaca tcctgaaata actcttcatc
      541 ttcttcatag gtacgcaaca actgcaaacg attcaaacgc gccatcatca gctcgttcga
      601 cttcaaagca gtcgtatagt aaatgagtac tttctgatat ttcaacaaat cgaaaatatc
      661 ctgattacgc atgctcaatt gcaaatcgtc ctcaatctga tcgactgtct tattgatttc
      721 acgtaagtga gccaaaaatt tcgtggcagt tttgaggaga ataagcagca aaaaccggta
      781 atgtttctgc gtttgaaggt tcttcacccg gttattctca aactcattca caatcacatt
      841 atcttccttg gtaatggtga taaagtaatc gttgttctga ataactccca ttggaatagt
      901 cacaaatgga gcttcgtcct tcttcccctg aaaatgtgga atcttaatca aggtaaacaa
      961 gacatcctcg tcgcgctcgg cacgtgcttt ttcgtcaata tctaatggat cgaggtggta
     1021 ttcttccgcc cagggaaatt cctgtatgag ccaggcaagc tcttcgtcac tcgggtcgac
     1081 aacgtttacc cagcaaccag gttccggctt ctgaatttct tccagtctgc ggtttgcggt
     1141 tttgaagatc gtaatcatcg tccatggcag cgggttaagg ccggtatttc tgaatactca
     1201 aagcgccgca aaaataggtt ttttattgaa agcaaatacg gttcaaatca gtaggaatta
     1261 ctaaaaattg ctataaaatc attacatctt tttccaactc ataatttata ctcttttact
     1321 ccagtctttc attattacta cattctttac ctaactcatt ctcatcttgt atcttcgctg
     1381 agatgaatct tcggtttctc ttcattttac tctttatttc atcttcggtt ttaagtcaaa
     1441 cttttgtcga aggtatggtg cgcgattcag ctcaaaaacg cccgcttgct tttgttagca
     1501 ttgccgttca aggttttcct gaagcaactg cgatgagcga tatcgatggt ttctttcgca
     1561 ttctgcttcc aaacggttat cctgcacagc tcaacttttc ctacatcggc taccgaagcg
     1621 aacaactaac attggaatcg gccaatggaa aatctaaact tcgggtagaa ctcactccat
     1681 cctctcttca acttcgaaca tttgaattcc ttgcgggtga aaatcctgca catcgaatca
     1741 ttcgagaaac ttggaaacgt cgcgaccaac acgatcccga aaaacttaag tcgtaccgct
     1801 gtcaaaccta taataaactc gtgctttccg gcaggcctga tacggcattt gatgctaaga
     1861 aaatcgaaga ccaacaaaag ctaaaatcct acgatagcct atttagcaag cagcatttgt
     1921 tcatgattga atcatcgaac gaacggcttt ttcgaaatgg gaaagtgaag gaaaatgtaa
     1981 tcggatcgaa ggtttcggga ttacaagaag cgtccgtttt catgctcgcg ctccagtttc
     2041 aaccctttag tttttatgca cctttggtgg aactaaacgg acaaaaactt ctcaacccaa
     2101 ttagtcgaaa cagcgaggac gtttactcat ttgtattaga agacaccttg tttagcggtc
     2161 gcgatagtgt ttacgtaatt cgatttcaac cgcgtaaaaa ttctaccatc gaaggactaa
     2221 aaggtgtgct ttacatttcg gctcccgatt atgccattca aaatgtggtg gcagaaccgg
     2281 cttctgataa ttctacgctc cgattacggg ttcagcagca gtacaaaaaa accgaaacgg
     2341 gtgtgtggtt tccggaacag ctcaacaccg atatgatttt ccttaccgtt aagcttacag
     2401 gctataagat gtacggggaa agtcgcacct acgtgagcaa acccgagatt aatccggaac
     2461 tttctgccaa acttttcgat gaagctgaac tggatgttca aaaaggatca tcagaaaaaa
     2521 gtcaggcatt ttggcaaacg gtgcggattg aagatttaac cgaacaggaa caacagacct
     2581 attaccaact cgacagcctg ttccgcgccg aaaatatggc tcctaaaatc aaagctgttg
     2641 aagccttggt tcgtggctac attccaatca aatggttcga tctagaactc aatcgcatca
     2701 tgcgtttcaa caaccatgaa ggctttcgtt taggtgctgg aggaattacg aacgacaaag
     2761 tttctcgaca ttttggaatt ggtggctaca tcgcatacgg attcaaagat caggtcacta
     2821 agtatggagc cgagtcgcgg atttatctct atccgagacg tgagttagaa cttcggatga
     2881 tgtatcaaca agatgtgtat gaatcgggct ttcaaacatt cgtgaacgac cgcaaaccct
     2941 atctcaccga acaagtgcgt caggttattg tccgaaacat gaatggggaa gaacggagcg
     3001 aagtcagttt aggatggcga tggctgaaat ttctacgaac tgatttgttc atccgccagg
     3061 cacatgtgaa aaccttattc gattaccaat atgattcgtt agcctcgaat acgcgatata
     3121 catggctaga acagggcgct tcgtttacgt attgttacaa ggaaaaattt taccgcaatg
     3181 ggaaacttaa aatttctttg ggtggaaaaa cgcctgtttt taggttgatg ctcacgcgcg
     3241 gttcagattt gagcttaggt ggaaaaaccg attatctccg agccgatttc cggatggaat
     3301 acgcctatca atggcgacgt attgggaaaa cggtaatgct actttcggct gggatgattg
     3361 atggaaatgt gccttacgcc cgactttata acggaaaagg aaatctttcg ggaaatgctg
     3421 atttgagagc tgtttcgacc aacagtttcg aaaccatgca catgaacgaa atcttaaccg
     3481 atcgttttgc aagtttgttt ttccaacacc atttgggttc attttttcat gtaggaacgt
     3541 ataaaccact gttcatcctt gttcataata tgatggttgg aacattggga aatacgaacc
     3601 gttcggctca aaaaatcatc agctttaccg ttcccgaaaa aggatacttc gaaagcggtt
     3661 tgctc
//
LOCUS       DAOOQW010000237         3647 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_30310_length_3647_cov_3.934577, whole genome shotgun sequence.
ACCESSION   DAOOQW010000237 DAOOQW010000000
VERSION     DAOOQW010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3647
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30310_length_3647_cov_3.934577"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3140
                     /locus_tag="PK281_10590"
     CDS             <1..3140
                     /locus_tag="PK281_10590"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HOY49833.1"
                     /translation="KYKAVVFLSTTGDVLDQYQQSVFKRFIQAGGGYMGIHAAADTEY
                     EWYWYGKLVGAYFKSHPKIQDAKFKKVKSDELVEGLPDDWMRKDELYNYKKISKDINV
                     LYTLDETTYEGGENGDNHPIVWYHEFDGGRSFYTGLGHTKESYVDPQYLDHIWMGLDY
                     VIGDSKLDYTKATTKLAPEENRFNKVVLASYFDEPTEMDILPDGRIIFLERKGAVKLY
                     DPKADSIALINTFNVNTKFEDGMLGLAHDPDFAKNNWLYIYYSTPSKSANILSRFTLD
                     GSKIDMASEKEMLEVATQRESCCHTGGSITFGPDNNLYVSTGDNTSPFESDGFSPSDE
                     TPGRSPFDAQKSSSNTNDLRGKILRIHPEADGTYSIPEGNLFPKGEEKTRGEIYVMGN
                     RNPYRISIDQKTGFLYWGEVGPDAGEDKETRGIRGYDEVNQAQKPGFFGWPYFVGGNY
                     AYNEYDFENKTSAPKHDALKPINNSPNNTGKLELPPVSPPFIWYPYAASPDFPLMKEG
                     SRNAMAGPVYYSEKYKGQSDAFPDYFDGKLIIYDWMRNWMRLVSMDQNGKITDIDPFM
                     EGVQFNNIIDMAFGPDGKLYTLEYGTRWFASNPDSRLSRIDFNRGNRAPLPKLTADKT
                     VGALPLVVNISAAGTIDPDGDAITYQLEANNQTLTSADGIFNVTFDKAGIYRPKLTVK
                     DSNGSSSSADITIVAGNEPPVVSLNLTTGNSQFYLAGGSVSYSASATDKEDGSSSDGS
                     IPAHRVLVTVDYLPQGYDMTKIAQGHQRAELPGKLLIAESDCKSCHLIDKKSAGPAYR
                     DVALKYKSDVKAIEKLSDKILKGGAGVWGETPMAAHPQVSKEDAMAMVEYILSLSDVK
                     DSKGLPLKGTAKFEPAPKEGITPTSAYIITAMYDDNGALGMPSISASQTSALRAPVLN
                     GADVVEFKGGTSKRSAGPNIVVDNVKHNSSVRYQDIDLTGVDKLQFTIAEIANVSKGG
                     EIEVYLDSPTGKKLGKVSFDKSPKIELRAGITARSTSITIEPLSGKHTFVLVFKNEKA
                     GNDNLFMLSRITLEK"
     gene            3156..>3647
                     /locus_tag="PK281_10595"
     CDS             3156..>3647
                     /locus_tag="PK281_10595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49834.1"
                     /translation="MNRREFIQRSSLTAAGLMVAPAFLQAGQKGKALGLQLYTLRSTI
                     GNDPKGVLQKVASYGYTELETYGYNDGKIFGMDFTEFCNYAKSLKMKVTSGHYGLNLI
                     KGDTWKKAVDDAKKNGQDFMVMPYLQEPDRKTIDQYKTIIADLNAAGEVCNAAGIRFQ
                     YHNH"
BASE COUNT         1147 a          673 c          830 g          997 t
ORIGIN      
        1 agaagtataa agcggttgtt ttcttaagca caacaggcga tgtgttagat caataccaac
       61 agtcagtttt caaacgtttc attcaggctg ggggtggcta catgggtatt cacgctgctg
      121 ctgatacgga atacgaatgg tattggtatg gaaaactggt tggtgcttac tttaagagcc
      181 atcctaaaat tcaggatgct aaattcaaga aagtaaaatc ggatgaatta gtcgaaggac
      241 ttccagatga ctggatgcgc aaagacgaac tctacaacta caagaaaatt tcgaaggata
      301 ttaacgtgtt gtacacgctg gatgaaacta cctatgaagg tggtgagaat ggagacaatc
      361 atcccattgt atggtaccat gagtttgatg gaggtcgttc attctatacc ggtttaggtc
      421 acaccaaaga aagttacgtt gatcctcagt acctcgatca tatttggatg ggattggatt
      481 atgtaatcgg tgatagtaaa ctggattaca ccaaagctac tactaagctt gcgccagagg
      541 aaaatcgatt taacaaagtt gtattggcga gctacttcga tgagcctacc gagatggaca
      601 tcttaccgga tggtcggatt atttttctgg aacgcaaagg tgctgtgaaa ctctacgatc
      661 caaaagcgga tagtatcgca ctcatcaata cctttaatgt taacaccaag tttgaagacg
      721 gtatgttggg tctggcacac gatccggatt ttgcaaagaa taactggttg tatatttact
      781 attcaacacc ctctaaatca gcaaacattt tatcacgctt taccttggat ggttcgaaga
      841 tcgatatggc ttctgagaaa gaaatgctgg aagtagccac tcaacgcgaa agctgttgcc
      901 ataccggtgg atcaattact ttcggtccgg ataataatct ctatgtgtct acaggcgata
      961 acacgagtcc gtttgaatcc gatggattta gtccttcaga tgaaaccccg ggaagatctc
     1021 ctttcgatgc acaaaaatcg tccagtaaca caaacgactt acgcggaaaa attttacgca
     1081 ttcatcctga agcagatggt acctattcca tcccggaagg aaatcttttc ccgaaaggtg
     1141 aagagaagac ccgcggtgaa atttatgtaa tgggtaatcg taatccctat cggatttcaa
     1201 tcgatcagaa aacaggattt ttgtattggg gtgaggtggg ccctgatgct ggtgaagata
     1261 aagagactag aggtattcgc ggatatgatg aagtgaacca ggctcagaaa ccaggatttt
     1321 ttggctggcc gtatttcgtt ggaggaaact atgcttacaa tgaatatgat tttgaaaata
     1381 aaacatccgc tccaaagcat gatgcattaa agccaataaa taattcacca aacaacacag
     1441 gtaaactaga actgccacca gtcagtcctc ccttcatttg gtatccgtat gctgcgtctc
     1501 ctgattttcc attgatgaaa gaaggtagta gaaatgccat ggccggaccg gtgtattatt
     1561 ccgaaaaata taaaggtcag tccgatgctt ttccagatta tttcgatggt aaactgatca
     1621 tctacgattg gatgcgtaat tggatgcgac tggtatcgat ggatcagaat ggaaaaatta
     1681 cagacattga ccccttcatg gaaggtgttc agtttaacaa tatcattgat atggcgtttg
     1741 gtccggatgg caagctttat acattggagt acggtacacg ttggtttgcc agcaatcctg
     1801 attcacgttt gtcgagaatt gattttaaca gaggtaatcg ggcaccgtta ccaaaactaa
     1861 cagccgataa aactgtaggc gctctgcctt tggttgtcaa tatttctgct gcaggaacaa
     1921 ttgatcctga tggtgatgcg attacctatc aactggaagc aaataatcag acactaacat
     1981 ctgctgatgg aatatttaat gtaacgtttg ataaagccgg aatctatcgt cctaaactta
     2041 cggttaagga ttcaaatgga tcaagctctt cggctgacat cacaattgtt gccggtaatg
     2101 agcctccggt tgtttcttta aatttaacga caggcaacag tcagttttat ttagcgggtg
     2161 gatcagtttc gtattcagct tccgctactg ataaagaaga tggaagttca tcggatggaa
     2221 gtatacctgc ccatcgggtt ttagtaacag tcgattacct tcctcaaggt tatgatatga
     2281 caaagattgc gcagggccac caacgagcag aactacccgg taaattgttg attgcagaga
     2341 gtgattgtaa atcatgtcac ttgattgata aaaaatcagc aggaccagcc tatcgcgatg
     2401 tagcgcttaa atataagagc gatgtaaaag caattgagaa attatctgat aagatactta
     2461 aaggaggtgc aggagtttgg ggagaaacgc caatggctgc tcatccgcaa gtatcaaaag
     2521 aagatgccat ggctatggta gagtatattt tatcgttgtc tgatgttaaa gattcaaagg
     2581 gattgccatt aaaaggtact gcgaaatttg aacctgcacc taaagaaggt atcacaccaa
     2641 catcagcata cattataact gccatgtatg atgataatgg tgcgttgggt atgccatcca
     2701 tttcagcttc tcaaacttca gctcttcggg ctcctgtgtt gaatggcgct gatgtagtgg
     2761 aatttaaagg cggcacaagt aagcgttctg ccggccctaa tatagtagtt gataacgtga
     2821 agcataattc atctgttcgt tatcaagaca ttgacttgac aggtgtcgat aaattgcaat
     2881 tcacgattgc tgagatagcg aatgtatcaa aaggaggaga gattgaagtt tatctggatt
     2941 caccaaccgg taagaaatta ggtaaggtaa gttttgataa atctccgaag attgagttac
     3001 gagcaggaat tacagcacgg tcaacttcaa taacgattga gcctttatca ggtaagcata
     3061 cttttgtgct ggtattcaaa aatgaaaaag cgggaaatga caacctgttt atgctaagtc
     3121 gaatcacttt agaaaaataa ctaaaccata ccaagatgaa cagaagagaa tttattcaac
     3181 gttcttccct cacagcagca gggttgatgg tggcacccgc ttttttacaa gcaggacaaa
     3241 aaggtaaagc attgggctta caactttaca ctttgcgaag cacaataggg aacgatccaa
     3301 agggtgtact acaaaaagta gcaagctatg gatatacaga actggaaacg tatgggtata
     3361 atgatggcaa aattttcggc atggacttta ccgagttttg caattatgca aagagtttga
     3421 aaatgaaagt aacaagcggt cattacggac tcaatctgat taaaggtgat acctggaaga
     3481 aagccgttga tgatgcaaag aaaaacgggc aagattttat ggtgatgcca tatcttcaag
     3541 agccagatcg taaaactatc gatcagtata aaactattat cgcagatttg aatgcagctg
     3601 gtgaagtatg caatgcagca ggtattcgct ttcaatacca caaccac
//
LOCUS       DAOOQW010000238         3636 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_30434_length_3636_cov_4.585311, whole genome shotgun sequence.
ACCESSION   DAOOQW010000238 DAOOQW010000000
VERSION     DAOOQW010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3636
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30434_length_3636_cov_4.585311"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1008)
                     /locus_tag="PK281_10600"
     CDS             complement(<1..1008)
                     /locus_tag="PK281_10600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49835.1"
                     /translation="MAWFLFFFWLGISLILLRKLSFFKESTIRPMPLSFLFLLKVLAG
                     IIVTLIYTYYYTDRSTADMYKYLDDAQVIASALPNHPTHYLKLMTGIDENAPELTPYY
                     SEMHNWSLHSETWFEYTQTKDFNLFSSNRIITRFNALLWPVTQGNIFTHVIFMCFISF
                     SGLIALFKLLIHYHPERKTRLVLLIFLWPSVLLWTSGVLKDGLIVAAFYLMIYWLDKF
                     ISKSGNRIVLFLAIVLALILLLITKYYVVVACIPALIAWMINHFRLTIPAFRMQLLVL
                     GICFITLIKMSYSGLNPDVISMLHDKREESLKAAILGNARNQLFVDNADPGIAGFIRK
                     IP"
     gene            1096..2052
                     /locus_tag="PK281_10605"
     CDS             1096..2052
                     /locus_tag="PK281_10605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49836.1"
                     /translation="MKHSLFILCFFLCLMPIKMCMAQDVIKQPAQLKFQATYFSPRTY
                     TDTTQKFNLQTYNAELAFSLGRIQLDTGIITFNGSIKQQIQPIQLFDSDPFRLLLTSS
                     IGIRTMYLDYKRIRGYLVFSNRLQIQSDEKNWSKQSPRYAGTLMYSRMNAKKWRWNYG
                     LAYSYVYGKGFLFPLLGFIYKPDSHQTWRAVLPFLFSYQYKSQSNIFTLAWHPAGGRN
                     AITSPWGTFDGENLQLNFRQSLLGFRYGRRLFQGGIIGLEAGICSKARITLDSDTWTN
                     NLQLSFEPGFYSGIFFRLPLYRVKGSNPKELFDDYELLLDNF"
     gene            complement(2112..3356)
                     /locus_tag="PK281_10610"
     CDS             complement(2112..3356)
                     /locus_tag="PK281_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007093112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOY49837.1"
                     /translation="MMDLFEKLKLNPGPLGSYAKEVHGYFTFPKLEGEIGPRMIFRGK
                     ERLNWSLNNYLGLANHPEVRKADAEGAAQYGAALPMGARMMSGNTDLHEQLERELADF
                     EKKEDAILVNYGYQGIMSAIDALVDRHDVIVYDSESHACIIDGVRLHMGKRYVYPHND
                     ISNLETQLERATKLVAESNGAILVITEGVFGMSGDQGKLKEIVELKKKFQFRLLVDDA
                     HGFGTMGKTGAGTSEEQDCMDGVDLYFGTFAKSMALIGGFIAGEKEVIEYLRYNMRSQ
                     IFAKALPMPLVVGSLKRLQLLRTQPELKDNLWKITNALQGKLRSEGFEIGKTQSPVTP
                     VFLNGSIPEAANLIFDLRENFNIFCSMVVYPVVPKGVIMLRLIPTAVHTLEDVDYTIK
                     AFSSIKDKLKAGHYERAMEKVI"
BASE COUNT         1172 a          706 c          630 g         1128 t
ORIGIN      
        1 cggaattttt ctgataaatc ctgctattcc tggatcagca ttatcgacaa ataactgatt
       61 tcgagcatta cccaaaatag cagcttttag agattcttca cgcttatcat ggagcataga
      121 tattacatcg ggatttaatc cactatacga cattttaatc aaagtgataa agcaaattcc
      181 taacactaat aattgcattc taaatgctgg aattgttaat ctaaaatgat ttatcatcca
      241 agcaattaaa gctggaatgc aggctactac aacgtaatat ttagtaatta ataaaagtat
      301 taatgcaagg acaattgcca gaaaaagcac aattcgatta cccgattttg agataaattt
      361 atctaaccaa taaatcatca aataaaaagc tgcgacaatt aatccatctt ttaaaactcc
      421 cgaagtccac aataagacag aaggccacaa gaaaatcaac aacacaagtc tggtttttcg
      481 ttcgggatga tagtgaataa gtaatttgaa taaagcaatt aaacctgaaa aggatatgaa
      541 gcacatgaaa atcacgtgtg tgaatatgtt tccttgtgta acaggccaaa gaagggcatt
      601 gaaacgtgtt attattctat tggaactaaa taaattaaag tctttagtct gagtatattc
      661 aaaccaagtt tcggaatgca aagaccaatt atgcatttca gaataatagg gtgttaattc
      721 aggggcgttt tcatcaatac ccgtcattag ttttaaataa tgtgttggat gattgggtag
      781 ggcagaagct ataacctgtg catcatccaa gtatttatac atatcggcag tactgcgatc
      841 ggtatagtag taggtataaa taagcgttac aataattcct gctaacacct ttaacaaaaa
      901 aaggaaactt aatggcatag gtcgaatggt gctttcctta aaaaaggata gcttccgtag
      961 gagaatcaga ctaattccca accagaaaaa aaagagaaac caagccatgg caacgaagat
     1021 acataaagct ataggcttaa atcgaatttt gatttcctcg attaaacgaa ttgttttttc
     1081 gtaactctgt gctaaatgaa gcactcactt tttattttat gttttttcct ctgtttaatg
     1141 cctattaaaa tgtgcatggc tcaagatgta atcaaacaac cagctcaact taagtttcaa
     1201 gctacttatt tttctcctcg aacttatacc gatacaactc agaaatttaa ccttcaaacc
     1261 tataatgccg aactagcttt tagtcttgga agaattcaat tagatactgg catcattacc
     1321 tttaatggca gtatcaaaca acaaatacaa cccattcaac ttttcgattc agatcctttt
     1381 cgattgctat taactagttc aattggaatt agaaccatgt atctcgatta caagcgcatt
     1441 cgtggttatc tcgtttttag taatcgactt caaatacaaa gcgatgagaa aaactggtct
     1501 aagcaatcac ctagatatgc aggaactctc atgtattcac gcatgaatgc taagaaatgg
     1561 agatggaatt atggattagc ctattcgtat gtgtacggca aaggatttct atttccattg
     1621 cttggcttta tttacaaacc cgattctcat caaacctggc gagcggtttt gcccttttta
     1681 ttcagctacc aatataaatc tcaatccaat attttcacgt tggcttggca tcctgcaggt
     1741 ggtcgaaatg ctatcacatc accctgggga acttttgatg gagaaaatct gcaactgaat
     1801 tttcgtcaat cattattagg ctttagatat ggtagacgac tctttcaagg aggaattatt
     1861 ggtttggaag ctggaatatg cagtaaagct cgtattacgt tggattccga cacttggaca
     1921 aacaacctac aactaagttt cgaacccggg ttttatagcg gaatattttt ccgacttcca
     1981 ttatatcgag tgaaaggttc gaatcccaaa gaattgtttg atgattatga attattactg
     2041 gataatttct aaaaacaaaa agccccgaaa atcaaaaatt ctcggggctt tcaattttat
     2101 gaaaagatta attaaattac tttttccatt gcacgttcgt aatgaccagc tttcaatttg
     2161 tctttaatgc tggagaatgc tttaatggtg taatctacat cttctagtgt atgaacagca
     2221 gtaggaatca agcgtaacat aatcacgcct tttggtacaa ctggataaac aaccatagag
     2281 cagaaaatat tgaagttttc gcgtaaatcg aaaatcaaat tagctgcttc tgggattgat
     2341 ccattcaaga atactggagt tacaggagat tgagtttttc caatttcaaa tccttctgaa
     2401 cgtaattttc cttgaagtgc attcgtgatt ttccacaaat tatctttcaa ttcgggttga
     2461 gtacgtagta attgaagtcg tttcaaagaa ccaactacta aaggcattgg tagcgctttt
     2521 gcgaaaattt gagaacgcat gttgtagcgt aaatactcaa tcacttcttt ttcaccagcg
     2581 ataaagccac caattagtgc catcgattta gcaaatgtac cgaagtacaa atccacacca
     2641 tccatgcaat cttgctcttc ggaagttcca gcacctgttt tgcccattgt accaaaaccg
     2701 tgtgcatcat caaccaataa gcggaattgg aactttttct taagctcaac aatttctttc
     2761 aacttacctt gatctccgct cataccgaaa actccttcgg taataaccaa aatagctccg
     2821 tttgattcag caaccaattt agtagcacgt tctaattgag tttctaagtt gctgatatca
     2881 ttgtgtggat atacataacg tttacccatg tgcaaacgaa caccgtcgat aatacacgca
     2941 tgagattcag aatcgtatac aatcacatcg tgacgatcaa ctaaagcgtc aattgccgac
     3001 atgatacctt gataaccgta gtttaccaaa atagcatctt ctttcttctc gaaatcagct
     3061 aattcgcgct cgagttgctc atgtaaatcg gtatttcccg acatcattcg agctcccatt
     3121 ggcaaagctg caccatattg agctgcacct tctgcgtccg ctttacgaac ttctggatga
     3181 ttggctaatc caagataatt gttcagactc caattgagtc gttctttgcc gcggaaaatc
     3241 atccgtggtc caatttcacc ttccagttta gggaatgtaa aatatccgtg tacttctttt
     3301 gcataagatc ctagcggacc tggattcaac tttagctttt caaataagtc catgatggga
     3361 gcgtgttaag caattcgccc gcaaaattaa gaaatttcat gtctcgcata ggaaaagtca
     3421 cgaacatccg tttgtcaaca accctttctg tatttttgtg ccatgagatt tttgaaacac
     3481 ccgcttattt ttctgctttt tattgctgtt atttcttcgt catgcagtga ttatcagaag
     3541 attctgaaaa gcaacgatta tgcagccaag tatgttttgg ctaaaaaact ttacgataaa
     3601 aaagactatc agaaggctta tccgctgttg gaagag
//
LOCUS       DAOOQW010000239         3609 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_30739_length_3609_cov_4.478897, whole genome shotgun sequence.
ACCESSION   DAOOQW010000239 DAOOQW010000000
VERSION     DAOOQW010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3609
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30739_length_3609_cov_4.478897"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="PK281_10615"
     CDS             complement(<1..489)
                     /locus_tag="PK281_10615"
                     /inference="COORDINATES: protein motif:HMM:NF012646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CUB domain-containing protein"
                     /protein_id="HOY49838.1"
                     /translation="MKKLSYIVPFLLLILFGISPAKAQTYIMGAAGGTITDCAGTIEN
                     PGGYGTYVDGLDVVQTICSGSGQCIQLTFTDFGTESGYDYLTVYDGNSTLGAVLGSYT
                     GATNPGVVTTSGASGGCMTLLFHSDGSVQSTGFSASISCIPCTPAIILGQGGSISACT
                     GII"
     gene            complement(492..881)
                     /locus_tag="PK281_10620"
     CDS             complement(492..881)
                     /locus_tag="PK281_10620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49839.1"
                     /translation="MKHTLLFLALFAGSALFAQQISSTTPQLDSRASRYYSAEQIAQM
                     DALNIAQLNFIFQNSWFVNTLKPCPECPAIDTATFDVYQYVREAKTRKRVYLTVPGNP
                     IDILSYQELDVELERIKNEMNTNSSQH"
     gene            complement(965..3142)
                     /locus_tag="PK281_10625"
     CDS             complement(965..3142)
                     /locus_tag="PK281_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010229937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HOY49840.1"
                     /translation="MRKLLILLAVVLHAGLFAQPLKDLSLQEIWASPTFRPSMVMGVS
                     PMQDGQHYSTLKIDESGVSIEKYAYSTGLKVGDIYTSASITLPGNKSIQFDSYTFSKD
                     EKKLLVPTETEPIYRHSSRSAYVVIDLEKKSASLLSTGKQQYADFSPDGKQIAFVREN
                     NLFVKDLISGKETAITKDGKQNEIINGATDWVYEEEFALSQAFFWSPDGSKIAYLRFD
                     EREVKEYNMQTYGSLYPGLYTFKYPKAGEDNSLVNVYVYSVSGNSSVKMQTGDDKNLY
                     FPRLQWTSNGSELCVTRMNRHQNHLELLLANPATGSIRTLLEEKSETYIDIHDNLRFL
                     ADGKRFIWSSELGGFNHLYLYGMDGKKISALSSGNFDITEFYGINEKSGTLYFQAAAP
                     TPMDRSLFTVKLTGKGQKNLLPVNGHQNPEFSSNFSYFVLNQSSANAPADYSLYSGEG
                     KLIRSLETNTILREKLATYRLSKKEFFSFTTSTGNALNGWMIKPSNFDASKKYPVFMT
                     VYGGPGHNTVENQWEGSNYMWYQYLAQKGYIVVSVDNRGTGNRGEAFKKSTYKQLGKL
                     ETEDQIEAAKYMAAQPYVDASRIGIQGWSYGGYMSSLCITKGADVFKMAIAVAPVTNW
                     RFYDSIYTERFMQTPEENESGYDDNSPINHVARLKGKYLLIHGTGDDNVHFQNSVEIV
                     TALVKANKQFDLFFYPDKNHGIYGGNTRLHLYTKMTDYLLENL"
     gene            3309..>3609
                     /locus_tag="PK281_10630"
     CDS             3309..>3609
                     /locus_tag="PK281_10630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49841.1"
                     /translation="MKINYWHFELPFQYPFSISGGRTKTHQPSLVVCIEHQGLKGFGE
                     APAILYYNIPVEKMIADLTDRLKELENFDFQHPESFGAFAFSLFPENSFLVCALDM"
BASE COUNT         1088 a          774 c          634 g         1113 t
ORIGIN      
        1 tatgatacct gtacatgctg atatagaacc tccttgacct aaaataatag caggtgtaca
       61 aggaatacat gaaatacttg cgctaaaacc tgtactttga actgaaccat cagaatgaaa
      121 taataaggtc atacatccgc ccgaagctcc tgatgtagtt acaactccag gattagttgc
      181 tcctgtatat gatcctaata ctgcacctaa tgtactattc ccatcataaa cagttaaata
      241 atcatagcct gattctgttc caaaatcagt aaaagtcaat tgtatacatt gtccactacc
      301 ggaacaaatt gtttgaacaa catcaagccc gtctacatag gtaccatatc cacctggatt
      361 ctcaattgta ccagcacaat ccgtaatggt acctcccgca gcacccatga tatatgtttg
      421 cgcttttgct ggacttattc caaataaaat aagtagcaaa aagggaacta tatagctaag
      481 ttttttcatg attagtgctg agaagaatta gtattcattt cattcttgat acgttccaat
      541 tccacatcta attcctgata agaaaggata tcaattggat tgcctggcac agtcaaatac
      601 actcgtttac gagtttttgc ttcacgaaca tactgataca catcaaatgt tgctgtatca
      661 attgcaggac attctggaca aggtttcaag gtattcacaa accaagaatt ctggaaaatg
      721 aagtttaact gagctatatt caaagcatcc atttgagcta tctgttcagc agaataatag
      781 cgagatgcac gagaatctaa ttgaggagtt gtgctagaga tttgctgagc aaataaggca
      841 ctgcccgcaa atagggctaa gaaaagcaat gtgtgtttca cagggtttgt gttgggatta
      901 caaatatatc aaaatatcaa ttcgaaaagc acgtatgttg attagcacct atttagcagg
      961 tatatcaaag gttttcgagt agataatcgg tcatttttgt gtacaaatgt aatcgcgtat
     1021 ttccaccata aattccatga ttcttatctg ggtaaaagaa caaatcaaac tgtttattcg
     1081 cttttaccaa agctgttact atctctaccg aattttggaa atggacatta tcatcacccg
     1141 ttccatgtat caataagtac ttgcctttta aacgtgctac atggttgatt ggtgagttat
     1201 catcatagcc cgattcattc tcctctggag tttgcatgaa acgttctgtg taaatagaat
     1261 cgtaaaatct ccagttcgtt accggtgcta ctgcaatggc cattttgaac acatctgcac
     1321 ctttggttat acataaagaa ctcatgtatc caccgtaact ccaaccttga attccaatac
     1381 gtgaagcatc tacatacggc tgtgcagcca tgtattttgc cgcttcaatc tgatcttccg
     1441 tttcgagctt tcctaattgc ttataagtcg atttcttaaa ggcttcaccc ctatttcctg
     1501 taccacgatt gtccaccgaa actacgatgt agcctttttg agccaagtat tgataccaca
     1561 tgtagttgga accttcccac tgattttcaa ccgtgttgtg tccaggacca ccataaacag
     1621 tcatgaaaac tggatacttt ttacttgcat caaaattaga tggcttaatc atccagccat
     1681 tcaacgcatt tcctgtgctc gttgtaaaac tgaaaaactc cttttttgat aatcgataag
     1741 tagctagctt ttcacgcaaa atcgtattcg tttccaatga acgaatcaac tttccttcac
     1801 ccgaatataa actgtaatca gccggagcat ttgcactgct ctgattcaac acgaaatagc
     1861 tgaaattact actaaactca ggattctgat gtccgtttac tggcaataaa ttcttttgtc
     1921 cttttccggt aagcttcacc gtaaaaagac ttctatccat aggcgttgga gctgccgctt
     1981 gaaaatacaa tgtgcctgac ttttcgttga taccataaaa ctccgtgata tcgaagttgc
     2041 cactactcaa tgcgctaatc ttctttccat ccatcccata gagatacaaa tggttaaatc
     2101 cacctagttc agatgaccag ataaaacgtt tgccatcagc caaaaagcga agattatcgt
     2161 gtatgtcgat ataggtttcg gatttttctt ccaacaatgt tcggatgctt cctgttgctg
     2221 gatttgccaa caataattct aaatgatttt gatgacgatt catgcgtgtt acacacaatt
     2281 ctgaaccatt cgaagtccac tgtagacgtg gaaaatacag gtttttatca tcgccagttt
     2341 gcattttcac tgagctgttt ccggatactg aatacacata cacattcacc aatgaattgt
     2401 cctctcctgc ttttggatat ttgaacgtgt ataatcccgg atacaaacta ccataagttt
     2461 gcatgttata ctctttcact tcccgctcat cgaagcgtaa ataggcgatt ttagaaccat
     2521 cgggcgacca gaaaaatgct tgactcaagg caaattcctc ctcataaacc caatcggtag
     2581 caccgttgat aatttcgttt tgctttccgt ctttggtgat ggctgtttct ttcccggaaa
     2641 tcaaatcttt cacgaaaaga ttattttcgc gcacaaaagc gatctgcttc ccatcaggag
     2701 aaaaatcagc atactgctgc tttcctgtgc tcagtaaact agccgatttc ttttctaaat
     2761 caatcaccac ataagcgcta cggctcgaat gacgataaat aggctctgtt tcggtaggta
     2821 ccaatagctt tttctcatcc ttactaaatg tgtacgaatc gaattgaatg gatttatttc
     2881 caggaagtgt aatactagca gaagtataaa tatcgcccac tttcaaaccc gttgaatagg
     2941 catatttctc aatactaacg cctgactcat ctattttcaa ggtgctgtaa tgctgtccgt
     3001 cttgcatggg tgaaacgccc ataaccatag atggtcgaaa cgttggactt gcccaaattt
     3061 cttgtaggga aagatctttc agaggctgag caaataagcc agcatgcagt actacagcta
     3121 agagaatcag caatttacgc atagatgttt tttttaagga ccgtaaaagt aacagaatca
     3181 taccgaacac aaatgcttac aggtaaattc ggaggaatca aatcattcat tcacattcgg
     3241 gtatgaacaa ctcctaagaa aaaccgaata ctggcagttg gtttacaata cttttgaatt
     3301 cgttccccat gaagatcaac tactggcatt tcgaattacc ttttcaatat ccattttcca
     3361 tttcgggtgg aagaactaaa acgcatcaac ctagcttggt tgtatgtatt gaacatcaag
     3421 gtttgaaagg atttggggaa gctccggcaa tactttacta caacattccg gtagaaaaaa
     3481 tgattgccga tttaaccgat cgattgaagg aattagaaaa cttcgatttt cagcatcctg
     3541 aatcgtttgg agcatttgca ttttccttat ttccggaaaa ctctttcctc gtttgtgctc
     3601 tcgatatgg
//
LOCUS       DAOOQW010000240         3589 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_30962_length_3589_cov_4.928693, whole genome shotgun sequence.
ACCESSION   DAOOQW010000240 DAOOQW010000000
VERSION     DAOOQW010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3589
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30962_length_3589_cov_4.928693"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(262..1017)
                     /locus_tag="PK281_10635"
     CDS             complement(262..1017)
                     /locus_tag="PK281_10635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49842.1"
                     /translation="MSADNAKNAKSKFKINQDAYVASSLLPNPNSFSRSLYKTKIVGQ
                     NLRKVTISLPSGEFTEIASSKVHLNIGLSIIEIGDFSTETTTLDPLTKALLNFSRLLI
                     DDSAVVAVKLRSTTELDLWWTNNGPAYTHVILIGHGSPNGIHFAVDGEVYHNRLSQIF
                     EKSNSQAVFISLCCETGCQSFSGEFSKNRFCKAIIAPRETIHAAVASQFSQAMLCWSL
                     VEGKTNKIAFNRAKSTIAGAVNMTMWKNGNSMQ"
     gene            complement(1096..2040)
                     /locus_tag="PK281_10640"
     CDS             complement(1096..2040)
                     /locus_tag="PK281_10640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49843.1"
                     /translation="MTILTLSSSLFCNSTTVDFRKSIKTATDNSKISNAKVAVLRSSV
                     EELSKSAFETTIIGSPTVKAMKQNDRSCDVAIKVEIGWSQSWIAKLKERLDLVGENIG
                     NKRSLYLLNDYAPTTLARWDNGTYVPGDTLSKSIVSFSTCKETKSCINWSDKNARERA
                     YRFLGNSCGGLPNYDGLKSIFPNSWAISTETFQMSRMDVAAEVLSNDMGRDRDWKPNH
                     FLAMENLSFFLSIEMLDDSGQIVYSKEIAIDPHLALSRNSRGEISGNVLVILSDARNA
                     IDLSISVPEEIMSKISKINVESHIKSEVLEIPQYYQRG"
     gene            complement(2101..3060)
                     /locus_tag="PK281_10645"
     CDS             complement(2101..3060)
                     /locus_tag="PK281_10645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49844.1"
                     /translation="MAAFSCPNTETPFDSLVCSNPALGVINTEYISLQKSIESRGDNK
                     NLMAEILLKDVRIRKTCSDVECARRWLEEQNRLLRSIDSSGRNNQLQTKNDSTIQSIP
                     DLRDTSGAPVTKKTAKKESTKKQAQHELRNDSGRGQLPEKGSSIQNRPEAELTPSQIR
                     KLMLFFLSLLAVATVIAIIRDIVRSRANEKYFNKKTKIGYGFALAGMASIGFGAIWTE
                     YSFVAFLISSIPYLTFLHGEYMSCPSCFRWWARAQTDYEELEKWIETKTVNRQDHTKD
                     RQGRIIATHHRKEQVLVECRKVKITYGCKHCGHTWHKIKTYKK"
     gene            complement(3303..>3589)
                     /locus_tag="PK281_10650"
     CDS             complement(3303..>3589)
                     /locus_tag="PK281_10650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49845.1"
                     /translation="AIDFITSTMDAMPPLYKVNFGTSKGLLEDIMYLSEFRLTINGWI
                     YFGPIDFDRFTLGGESRIPIPEILIDDNFIDRVLSAVFDGIERNSITSLE"
BASE COUNT          983 a          740 c          689 g         1177 t
ORIGIN      
        1 gggactttca gtccctgtgg cgaacccgcc gactttagtc ggcggttgtt cagttccaaa
       61 atctgattca attggccaat ttcccgtcat attaactcct tatgcatcct taaaaatcgc
      121 atacattgtg gtgttagatg cttttattaa atggctaatt tcctgttcag gaaattgaaa
      181 tataggcgat atcattaggg cttctggcat cgcccttttt tagaaactcg ttcattaaat
      241 tgttttgcat agtgtttagt gttattgcat tgaattgcca tttttccaca tcgtcatgtt
      301 tactgcacca gctatggtgc tttttgctcg attaaaagcg attttatttg ttttgccctc
      361 aactaagctc cagcaaagca tggcttgtga gaattgtgag gccaccgcag catgaattgt
      421 ttctcttggc gctataatag ctttgcagaa ccggttttta gaaaattcac ctgaaaacga
      481 ttggcagcca gtctcgcagc aaagagaaat aaatactgcc tgtgaatttg atttttcaaa
      541 aatttgggag agtctgttgt gatagacctc cccatcaaca gcaaaatgaa taccattagg
      601 tgatccatgc ccaataagta taacatgcgt gtatgctggg ccattattgg tccaccacaa
      661 gtctagttca gtggtggaac gcaatttgac tgctacgact gctgaatcat caatcaataa
      721 tcgtgaaaaa tttagcaacg cttttgttaa cggatctagc gttgtggttt ctgtcgaaaa
      781 atcgccaatt tcgatgatgg acagcccaat atttaaatgc acttttgagc tagctatttc
      841 tgtaaattct ccacttggca gtgaaatcgt tacctttctc aaattttgtc ctacgatttt
      901 tgtcttgtaa agtgaacggg aaaagctgtt cggatttggc aagagtgatg aagctacata
      961 tgcatcttga ttaattttga attttgactt tgcattttta gcattatcag cactcatatt
     1021 gccatcctta ttgttgcgac aaagcaattg ccatttctaa tcgaagcaaa atgatagcac
     1081 gcactagctc gaagctcagc ctctttgata gtattgagga atctccaaaa cttccgattt
     1141 gatatgtgat tccacgttga tcttcgaaat ttttgacata atttcctctg gaacgctgat
     1201 cgacaaatct atggcatttc ttgcatctga aaggattact aatacattgc ctgagatttc
     1261 tccgcgtgag tttctggaaa gcgctagatg tgggtcgatg gcgatttctt tgctatatac
     1321 tatttgccct gaatcatcca gcatttctat actgaggaaa aatgacaaat tttccatcgc
     1381 taaaaagtgg tttggcttcc aatcgcgatc cctgcccata tcattagaaa gtacctcggc
     1441 tgcaacatcc attcttgaca tttggaatgt ttccgttgat attgcccatg agtttggaaa
     1501 gattgattta agaccatcat aattcggaag accaccacat gaattaccta aaaatcgata
     1561 tgctctttct cttgcatttt tgtcgctcca gttaatacag gatttagttt ctttgcaagt
     1621 cgaaaaggat acaatcgatt tcgatagggt atcgcctgga acatatgttc cattatccca
     1681 gcgagctaat gttgtcggcg cataatcatt cagcaaatac aatgatcttt tgtttccgat
     1741 gttttctcca acaagatcca atcgctcttt caattttgct atccatgact ggctccatcc
     1801 aatctcaact ttaattgcca cgtcgcatga acgatcattt tgcttcattg cttttacggt
     1861 tggacttcca ataatggtag tttcaaatgc tgatttcgaa agctcttcca cgctagaacg
     1921 caatacagca actttagcat ttgatatctt agaattatcg gtcgcagttt tgatggattt
     1981 ccgaaaatcg actgtggtgc tattgcaaaa caatgatgaa gaaagcgtta aaatagtcat
     2041 gaatgaacgt tttgttttgg aaaaagaatg cattgcgaat ctcatgtaaa tctccttatg
     2101 ttatttctta tacgttttga ttttgtgcca tgtatgacca cagtgtttgc atccgtaagt
     2161 gattttaact ttacggcact caacaagaac ctgttccttc ctgtggtgtg ttgcgattat
     2221 tctaccttgc ctatccttgg tgtgatcctg tctatttaca gtttttgttt ctatccattt
     2281 ttcaagctct tcgtaatcag tttgagccct agcccaccaa cgaaaacagg atggacagct
     2341 catatactcg ccgtgcaaaa atgtcaaata cggaatcgat gaaatgagga aggcaacaaa
     2401 tgagtattca gtccatattg caccaaatcc aatactcgcc attccagcca aggcgaatcc
     2461 atatccgatt ttggttttct tattaaaata cttttcattg gctctgcttc taacgatatc
     2521 acgaattatt gcaattactg ttgctacggc aaggagacta aggaaaaata gcatgagctt
     2581 acgaatttgg gatggagtca attcagcttc cggtctgttt tgaatcgaag aacccttttc
     2641 aggaagttgg ccccttccag aatcattacg tagctcatgt tgagcttgtt ttttggtgga
     2701 ctcctttttt gcagtctttt ttgtgactgg tgcaccagaa gtatctctca aatcaggaat
     2761 agactgaatt gtagaatcat tctttgtctg aagctggttg tttcgaccgg aagaatcgat
     2821 actccgcaat aagcgatttt gttcttcaag ccatcttctt gcacactcaa catctgaaca
     2881 tgttttccgt atgcgaacat cctttaggag aatctctgcc atcaaattct tattgtcgcc
     2941 tcttgattca atagatttct gaagagatat atattctgta ttaatcactc caagagccgg
     3001 attggagcat acaagggaat caaaaggagt ttctgtattt gggcaagaaa atgcagccaa
     3061 aaaagcaatt atccaataaa tcattatcct cacacctttc tgtttgttct ttagttaagc
     3121 gactaggctg ttctgtggtt tattcgattt gatttgcagc tcatccaccc ggagggtttc
     3181 cctaacgccc cctgttcacc tagtcgcgca catcggcata acaacgaggc gcgatctagg
     3241 tggaacgggc gtgttaggga cgatcagctg ctcatctcat gatgttcaga caaattttgg
     3301 ctctattcta agctggttat cgaattgcgt tctattccat cgaatacagc ggaaagtact
     3361 cgatcaataa aattatcatc gattaggatt tctggtatag gaattcttga ctcaccaccc
     3421 aaagtgaatc gatcaaaatc aatcggacca aaatatatcc atccgtttat ggttaaacga
     3481 aattctgaca gatacataat atcttcaaga agtcctttgc ttgtgccaaa gtttaccttg
     3541 tagaggggtg gcattgcatc catggtgctc gtaataaaat ctattgcat
//
LOCUS       DAOOQW010000241         3589 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_30964_length_3589_cov_4.345784, whole genome shotgun sequence.
ACCESSION   DAOOQW010000241 DAOOQW010000000
VERSION     DAOOQW010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3589
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30964_length_3589_cov_4.345784"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2264
                     /locus_tag="PK281_10655"
     CDS             <1..2264
                     /locus_tag="PK281_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013072012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HOY49846.1"
                     /translation="EARLFKTSLIGTEHLLLAILKDEDNIVTKTLEKFNVDYTTVRDE
                     LKNLLTEEPRAQASNNPLDDDNDDDAGSFGGGGTKKVTDTKSKTPVLDNFGRDLTKAA
                     DEGKLDPIVGREKEIERVSQILSRRKKNNPILIGEPGVGKSAIAEGLALRIVQKKVSR
                     VLFDKRVITLDLASLVAGTKYRGQFEERMKAVLNELEKSPDVILFIDEIHTIVGAGGA
                     SGSLDASNMFKPALARGEIQCIGATTLDEYRQYIEKDGALERRFQKVIVDPTSEEETF
                     QILNNIKGKYEDHHNVIYTPEALKACVTLTQRYVSDRHLPDKAIDALDEAGSRVHITN
                     IHVPQNILDIESKIEEVKAEKIQVVKSQKYEEAARLRDKEKTLIEQLDAAKKAWEEET
                     RNNRQTVTEDNVAEVVAMMTGVPTQRIAQNESSRLVKMYDEIQGKVIGQDEAIKKVVK
                     AIQRNRAGLKDPNRPIGSFIFLGPTGVGKTQLAKVLSKYLFDSDDALIRIDMSEYMEK
                     FAVSRLIGAPPGYVGYEEGGQLTEKVRRKPYSVVLLDEIEKAHPDVFNLLLQVLDDGQ
                     LTDSLGRKIDFKNAIIIMTSNIGSRQLKDFGQGVGFTTTAKATSASDHDRGVVESALK
                     KAFAPEFLNRVDDVIMFNSLSKENINEIIDIELAHLYRRIENMGYKIKLEDSARDFIV
                     EKGYDSQFGARPLKRAIQKYVEDPLAEHIIGSTLTEGDSINLSYDEELKEIQVKISKP
                     KKKKKSEDQAESE"
     gene            complement(2443..3210)
                     /locus_tag="PK281_10660"
     CDS             complement(2443..3210)
                     /locus_tag="PK281_10660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49847.1"
                     /translation="MKNRPFSCRTICLSLLALSLICFSSQANGQEEGITPVKQRTIPF
                     SIRFDGGLGFLTTPRAMKLTFNSVGDANVGTFFHIAKGVNVGLNFRYTGYQVSRNASN
                     FNDLDTIKGANGTTVISVPIHTTHNMFSPGITLGYDKWISGYSLFNFNFCTAYSLVRY
                     ANIRSLEKDYPNPPNKSVYNYNALLIEPAVNFMYFFEDHAAMSIKVSYSRLFAGFHPE
                     QIALDGGAISYEKADLGGQIQFISVGLGFVYSFKRIE"
     gene            complement(3210..>3589)
                     /locus_tag="PK281_10665"
     CDS             complement(3210..>3589)
                     /locus_tag="PK281_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009751608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOY49848.1"
                     /translation="VALTYIAAQRTFPDYSDMAEAKAMLESIARSFGYHYGKAKHVRV
                     NTISQSPTWTTAGSGVPGFKEFYNYADTMAPLGNASALDCANYCISLFSDYTRMVTMQ
                     NLFHDGGFSNMGINAAMVVPKED"
BASE COUNT         1182 a          641 c          784 g          982 t
ORIGIN      
        1 tagaagctcg gttattcaag actagtttaa taggtactga acacttattg ctagccatct
       61 taaaagatga agacaatatt gtcacaaaaa ccttagaaaa atttaatgtg gattatacaa
      121 ctgtacgaga tgagctgaag aatttgctca cagaagaacc acgtgcccaa gcttccaaca
      181 atccgctcga cgatgataat gacgacgatg ctggatcatt tggtggcggc ggaaccaaaa
      241 aagtgactga tacaaaatcg aaaactccgg ttctagataa tttcggtcgc gatttaacaa
      301 aagctgccga tgaaggaaaa cttgacccaa ttgttggtcg agaaaaagaa atcgaacgtg
      361 tttctcaaat cctttctcgt agaaagaaaa acaatccaat tctaattggt gaaccaggtg
      421 ttggtaagtc ggctattgct gaaggacttg cacttcgtat cgttcaaaag aaagtatctc
      481 gtgttttatt cgataagcga gttatcacat tagacttagc ttctttagtt gctggaacca
      541 aatatcgggg tcagtttgaa gagcgtatga aagcggtatt gaatgaatta gagaaatcgc
      601 cagatgtgat tctattcatt gatgagattc atacaatcgt tggcgctggt ggagcaagtg
      661 gatcattaga tgcatctaat atgttcaaac cagcattggc tcgtggagaa attcaatgta
      721 ttggtgctac tacgctagat gaatatcgtc agtatattga gaaagatgga gcgttagaac
      781 gtcgtttcca gaaggtgata gttgatccaa cttcggaaga agaaactttc cagattctca
      841 ataacatcaa aggaaaatac gaagatcacc acaacgtaat ttacactcct gaagctttaa
      901 aagcctgtgt aacattgact caacgttatg taagcgatcg tcatttacca gataaagcga
      961 ttgatgcttt ggatgaagca ggttcacgtg tacacattac taatatacat gtaccacaaa
     1021 acatcctcga tattgaatct aaaatcgaag aagtaaaagc tgagaaaatt caggtggtga
     1081 aaagccagaa atatgaagag gctgcccgac ttcgcgataa agagaaaacg ctcattgaac
     1141 aactcgatgc agcgaaaaaa gcttgggaag aagaaacccg aaataatcga caaacagtta
     1201 cagaggataa tgttgccgaa gttgttgcga tgatgacggg tgttccaacg caacgaattg
     1261 ctcaaaacga aagttctcgt ttggttaaga tgtacgatga aatccaaggg aaagtaatcg
     1321 gacaagatga ggccattaag aaagtagtaa aagccattca acggaatcgt gctggattga
     1381 aagatccgaa tcgtccgatt ggttcattta tcttcttggg acctactggt gtgggtaaaa
     1441 ctcaattagc taaagtgctt tcgaagtact tattcgatag tgatgatgca ctcattcgta
     1501 ttgatatgag tgagtacatg gagaaatttg ctgtttctcg tttgattgga gcgcctccag
     1561 gatatgttgg ttatgaagaa ggtggacagt taacagaaaa agttcgtcgc aaaccatatt
     1621 cagttgtctt attggatgag attgaaaaag ctcacccgga tgtattcaac ttgttattgc
     1681 aggttttgga tgatggtcaa ttgactgata gtttgggtcg taaaatcgat ttcaagaatg
     1741 ccatcatcat catgacttcg aacattggtt cacgtcagtt gaaagatttc ggacaaggag
     1801 ttggtttcac aactactgca aaagcaactt cagcttctga tcacgatcgc ggtgtggttg
     1861 aaagtgcatt gaaaaaagca tttgctcctg aattcttgaa tcgtgtggat gatgtgatta
     1921 tgtttaattc attaagcaaa gaaaacatca acgagattat tgatattgaa ttagctcatt
     1981 tgtatcgtcg tatagaaaat atgggataca aaatcaaatt ggaagattcg gctcgtgatt
     2041 tcattgtgga aaaaggatac gattctcaat ttggtgctcg tccgctaaaa cgtgcgattc
     2101 agaaatacgt agaagatcct ttggcagaac atatcattgg ttctacccta accgaaggag
     2161 attctatcaa cctatcgtat gatgaggaat tgaaagagat tcaggtaaaa atcagcaaac
     2221 cgaaaaagaa gaagaaatcg gaagatcaag ctgaatctga ataggaatta gtactaaaac
     2281 aaaagccccg gtagagttga atctatcggg gctttttatt tcaattcagt tgagtaaagt
     2341 aaatattaag tctagcaatt tgtgctagga attgaactaa tatgccttcg ctaaagctat
     2401 ggcattgatg gaggctactg cgaaaaccag gaaaccacct aattactcaa tacgcttaaa
     2461 ggaatacaca aaacctaatc ctactgagat gaactgaatt tgtccaccta gatctgcttt
     2521 ttcatacgat atagcgccac catcgagggc aatctgttca ggatgaaaac ctgcaaacaa
     2581 gcgtgaatag gaaactttga tggacatggc agcatgatct tcgaagaaat acatgaagtt
     2641 cacagctggc tcgatcaata aagcattgta gttataaacc gatttatttg gaggatttgg
     2701 atagtctttt tctaagctgc gaatattggc ataacgtact aatgaatagg cagtgcagaa
     2761 attaaaatta aacaaggaat aaccactgat ccacttatcg taaccgagcg taattcctgg
     2821 actaaacata ttatgtgtag tgtgaatggg aactgagatt acagttgtcc catttgctcc
     2881 tttaatagta tcgagatcgt tgaaattaga agcgtttcga gaaacctgat atccagtgta
     2941 acgaaaattc aaaccaacat ttacaccttt agcgatgtga aaaaaggtgc ccacattcgc
     3001 atcacctaca ctgttaaagg taagtttcat ggcgcgaggc gtagttagaa aacctaatcc
     3061 accatcgaac cgaattgaaa atggaatggt acgttgtttt actggagtaa taccttcctc
     3121 ctgcccgttc gcttgggaac taaaacaaat taaactcaaa gccagtaggc tcaggcaaat
     3181 tgtacgacag gagaaaggtc tatttttcat taatcttctt ttggaacaac catagctgca
     3241 ttgattccca tgtttgagaa tcctccatcg tggaaaagat tctgcatggt aaccatacgt
     3301 gtatagtcgg agaataggct gatgcaataa ttagcacaat ctaaagctga agcgtttcca
     3361 agtggtgcca tggtatcggc atagttgtaa aactctttga agcccggtac gccgcttcca
     3421 gcagtagtcc atgttggaga ttgcgaaatg gtatttacac gcacatgttt cgctttaccg
     3481 taatggtaac caaaactgcg ggcaatcgat tcaagcatcg ctttcgcttc ggccatatcg
     3541 ctataatctg ggaaagtacg ttgagcagca atgtaggtta gcgcaacca
//
LOCUS       DAOOQW010000242         3564 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_31273_length_3564_cov_3.893702, whole genome shotgun sequence.
ACCESSION   DAOOQW010000242 DAOOQW010000000
VERSION     DAOOQW010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3564
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31273_length_3564_cov_3.893702"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="PK281_10670"
     CDS             complement(<1..241)
                     /locus_tag="PK281_10670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49849.1"
                     /translation="MIRAAVLVLALIAFPAITTAQTSKDFTLLKLHSLAQKIGDNNPF
                     SEYNSEQMKKVRPIKIKGFDFVKLIQATDNDKREFW"
     gene            complement(619..1302)
                     /locus_tag="PK281_10675"
     CDS             complement(619..1302)
                     /locus_tag="PK281_10675"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HOY49850.1"
                     /translation="MDHIIHQLKQSLDDDFLSKPERKSLSQALEDQFLDTNQITFLRT
                     KVFELANEKVNDKNYRFILEWIKDASNVLSNYPLEKSKSDDSYFSPGDHCRNAIVQHL
                     NLAKKRLKICVFTISDDIITEAILRAKRRGVDVMVLTDNDKSFDLGSDIEKLSKAGIS
                     VKMDQTSDHMHHKFMIVDTDAVLSGSYNWTRSAARFNHENVVVMKEEGVVKSFMSEFD
                     KLWSEMSDY"
     gene            complement(1309..2493)
                     /locus_tag="PK281_10680"
     CDS             complement(1309..2493)
                     /locus_tag="PK281_10680"
                     /EC_number="4.2.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA];
                     GO_process: GO:0071268 - homocysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HOY49851.1"
                     /translation="MEETERHFETDAIREQHERSDFREHSVPLYLTSSFVFDDAEQAR
                     AMFADEIPGNIYSRYSNPNTNEFIAKLCLMEGTEDGIATASGMAAMYSSMAALLKSGD
                     HVLACRSVFGSTHQILNTIFPKFGITYSYADISEPETWESKIQPNTRMIFVETPSNPA
                     LDLIDLEWLGKLAKKHNVILNVDNCFATPYLQQPAKWGAHIVTHSATKFMDGQGRVIG
                     GAILGTKELIKEIRFFTRHTGPSMSPFNAWVLSKSLETLAVRMDRHCENSLTLAKWLE
                     GNKQVVKVKYPFLPSHPQHALAKKQMKLGGGIVTFEIKDGLDQGRRFLNALKMISHTP
                     NLGDTRTIAIHPASTTHSKLTEEERQAVGITPGLIRIAVGLEDVRDIIADISQAIEAS
                     KS"
     gene            complement(2510..2932)
                     /locus_tag="PK281_10685"
     CDS             complement(2510..2932)
                     /locus_tag="PK281_10685"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOY49852.1"
                     /translation="MITIELNRVNDAFLMEATNELGNSVRMDGSPGIGGSDLAMRPMQ
                     MLLAALGGCSSIDVISILKKQRQDLKDLKITVTGEREQNVEPSLYTEIHAHFKFYGNL
                     DADKVEKAVMLSMEKYCSVAKTLEKTAKVTHTFEIINS"
     gene            complement(2929..>3564)
                     /locus_tag="PK281_10690"
     CDS             complement(2929..>3564)
                     /locus_tag="PK281_10690"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49853.1"
                     /translation="GSANTAVRSCYIAAVLYLTIAMLPLFISLCTKHLYPQYAEGDTQ
                     LALPNMVLAHTSLPIQILFFGSLLSAIMSTTSSAILAPASILSENVMKPLLGDKYTDK
                     QLLMTTRISVLVFSTIATVMACMRSNIYELVGESSILSLVSLFVPLVMGLYWKRANTT
                     GALLSMVLGIVTWIIFEIYETSWPSLIPATLVSLAGMVVGSLARPMKTETV"
BASE COUNT         1023 a          803 c          654 g         1084 t
ORIGIN      
        1 cccaaaactc ccttttatca ttgtcggtag cttgaattaa tttaacaaaa tcaaaacctt
       61 tgatttttat cggccgtact ttcttcattt gctcagagtt gtattcacta aagggattat
      121 tatctccaat tttttgtgcc aaagaatgta actttaaaag cgtaaaatct ttactagttt
      181 gagccgtagt tattgcagga aaagcaataa gtgccaaaac taaaactgct gctcgaatca
      241 tcttacttta ctttaatcta ttctttacaa atgcaaaatc tggcaatacc ttcggtgtga
      301 gaagtgtggt agactagcct gtgtttgcga ggaagcgtga agcgcctcgc tgaacatgtt
      361 gacgaggcaa tgtctcatcc agcccatggc gggaccccca accacgaagt gctatagtgg
      421 aggcaatggt ccactatatt aagtttgttg caacattttc tcgttcattc gtatttcatt
      481 gcaaggggat tgcttcactc aatcccacac cccacgcttc cctgcctgcc ggcaggcagg
      541 agttcgcaaa cgcgatactt gcagcgaagc acacccaagc ccacacaccc gattaactat
      601 taacaattaa ccaataactt agtaatccga catctcactc cacaacttat caaactcact
      661 cataaacgac ttcaccactc cttcttcttt cataacaaca acattctcgt ggttaaatcg
      721 cgcggcactt cgtgtccagt tatagcttcc acttaatacg gcatcggtat ccacgatcat
      781 aaatttatga tgcatgtgat cactcgtctg atccatcttt accgaaattc ctgcttttga
      841 aagcttttca atatccgaac ccagatcaaa cgatttatca ttatccgtaa gcaccatcac
      901 gtccactccc cttctctttg cgcgtaatat ggcttctgtt atgatgtcat cactaatggt
      961 aaagacacaa atcttcaatc gtttttttgc aaggttcagg tgctgaacaa tagcattgcg
     1021 acaatgatcg cccggactaa aataactatc gtccgatttc gatttttcca atggatagtt
     1081 gctcaataca ttgctggcat ctttaatcca ttccagtata aaccgatagt ttttatcatt
     1141 taccttctcg ttcgctaact caaatacttt ggttcgaagg aaggtaattt gattcgtatc
     1201 aaggaattga tcttctaaag cttgcgacaa tgattttctt tctggcttgg aaagaaaatc
     1261 atcgtcaaga ctttgtttta attgatgaat gatgtgatcc attatccatc aactttttga
     1321 tgcttcaatg gcttgagaaa tatcagcaat gatatcacga acatcttcaa gaccaactgc
     1381 aatccggata agtccgggag taatacctac tgcctgacgt tcttcttcgg ttaacttcga
     1441 atgggttgtc gatgccggat gtatggcaat ggttctggta tcacctaagt ttggagtatg
     1501 tgaaatcatt ttcaatgcat tgagaaatcg tcttccctga tcaagtccat cctttatttc
     1561 gaaggtaaca atgccaccgc ctaacttcat ttgttttttt gctaatgcat gttgaggatg
     1621 cgatggtaga aatggatatt tcactttcac tacctgctta ttgccttcaa gccacttagc
     1681 aagcgttaac gaattttcgc aatggcgatc catacgcacg gccagtgtct ctaaactttt
     1741 agaaagtacc cacgcattaa aaggcgacat cgaaggacct gtgtgccgtg taaagaatcg
     1801 gatttctttt atcaattctt ttgtgcccaa tatagctcca ccaattactc taccttgtcc
     1861 atccatgaat ttggtagcgg aatgcgtaac aatgtgcgct ccccattttg caggttgttg
     1921 taagtaaggt gtagcaaaac aattgtcaac gttcagaatc acattatgct tcttcgccaa
     1981 tttacccaac cattctaaat caataagatc taaggctgga ttggatggtg tctccacaaa
     2041 aatcatccgc gtgttgggtt gaatttttga ttcccatgtt tctggttcac tgatatcagc
     2101 ataactatag gtaatgccaa actttggaaa tatagtattg agtatttgat gtgttgatcc
     2161 aaaaactgaa cggcacgcca aaacatgatc acctgatttt aataatgcgg ccatgctgct
     2221 atacattgca gccatacctg aagcagtagc aatgccgtct tcagttcctt ccatcaaaca
     2281 aagctttgcg ataaactcat ttgtgtttgg attggaatat cggctgtaaa tattgccagg
     2341 aatttcatcg gcaaacatag cgcgagcctg ctcagcatca tcaaatacaa aactggaagt
     2401 taggtatagt ggcacagaat gttcacgaaa atcagaacgc tcgtgttgtt cgcgaatagc
     2461 atcagtctca aagtgtcttt cagtttcttc catcgatgta tataaaaaat tatgaattaa
     2521 tgatttcaaa agtgtgggta accttggcgg ttttctcaag tgtcttggct acagaacaat
     2581 atttttccat ggaaagcatt accgctttct ctaccttatc tgcatccagg tttccataga
     2641 atttaaaatg ggcgtgaatt tcggtatata atgacggctc cacattctgt tcgcgctcgc
     2701 ctgttacggt aatcttcaga tcctttaaat cctgtcgttg ttttttcaaa atgctgatca
     2761 catcaatcga gctgcaaccc cctaatgccg ccaacaacat ttgcattggg cgcatggcta
     2821 aatcacttcc tccaatacca ggggaaccat ccatcctcac ggaattacct aattcattcg
     2881 ttgcctccat caggaaggca tcgtttactc tgtttaattc tatcgtaatc atactgtttc
     2941 tgttttcatt ggtctcgcta agctacccac caccatacct gccaaactca ctaatgttgc
     3001 aggtatcaaa cttggccatg atgtttcata aatttcaaat ataatccaag tcactattcc
     3061 aagcaccatt gaaagtaaag ctccagttgt attggcgcgt ttccaataca atcccattac
     3121 cagcggaacg aaaagcgaaa ctaaacttaa aatagatgac tcacccacca attcataaat
     3181 gtttgatcgc atacatgcca ttacagtggc aatggtgctg aatactaata ccgaaatgcg
     3241 agtcgtcatg agcagttgct tatccgtgta cttatcacca agaagtggct tcattacgtt
     3301 ctctgataaa atggatgcgg gcgcaagaat tgctgaactg gttgtactca ttatggctga
     3361 tagcaatgaa ccaaaaaaca aaatctgaat aggaagtgaa gtatgtgcca gcaccatatt
     3421 aggcagtgca agttgggtat caccttctgc atattgcgga tacaaatgct tggtgcacaa
     3481 acttataaag agcggtagca ttgcaatggt taaatacaaa acagcggcaa tgtagcacga
     3541 ccttacagca gtatttgctg agcc
//
LOCUS       DAOOQW010000243         3532 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_31644_length_3532_cov_3.938453, whole genome shotgun sequence.
ACCESSION   DAOOQW010000243 DAOOQW010000000
VERSION     DAOOQW010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3532
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31644_length_3532_cov_3.938453"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1026)
                     /locus_tag="PK281_10695"
     CDS             complement(<1..1026)
                     /locus_tag="PK281_10695"
                     /inference="COORDINATES: protein motif:HMM:TIGR04519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-variant-translocated molybdopterin
                     oxidoreductase"
                     /protein_id="HOY49854.1"
                     /translation="MSNSKKYWKGIEELQATPEFVKLQQNEFAEDLPVDQFLGDERIV
                     DSSTNRRDFLKFLGFSVTAASLAACETPVRKAIPYLNKPEEITPGVANYYASTYWDGN
                     DYAGVLVKTREGRPIKVDGNELCSITKGGSHARVQASVLSLYDSARIKGAMLNNKDSD
                     WKAVDAEVTSKLASIAAAGGAIRILTSSLISPSMKSAIAEFTTKYPTTQVVTYDSISY
                     KGIADSNLASFGKSFIPTYNFDKADFIVGIGCDFLANWLSPVEHARQYAETRKLNKDE
                     KKMSRHVQIESLLTVTGSNADKRIALKPSQLGLAVVNLYNKLTGSSLPSKSLDAKDAA
                     IAELAKEL"
     gene            complement(1064..2326)
                     /locus_tag="PK281_10700"
     CDS             complement(1064..2326)
                     /locus_tag="PK281_10700"
                     /inference="COORDINATES: protein motif:HMM:NF025875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HOY49855.1"
                     /translation="MHLFRSHILRVLAVFFAVALSSNTTYAQDGAKLFKGNCASCHKP
                     TDQKLVGPGLKGVKSRWTDEAKLISWIKNSAEFLKTGDAYANKLFADYGNSPMPAQAL
                     SDAEILAVINWADKGGDAPVAATPTDGNAAGTSITEAKPASNETYFYVLLGLGVLLIV
                     LINVLSTVKASLETLVNEKNGIHIEPKPVLGPIERFRFWASQNKKIVAIASLLLVSWV
                     LKQGWDGLMGIGVYQGYAPEQPIKFSHEIHAGQNKIACVYCHSGVEKSKHANIPSANV
                     CMNCHAYIQQGATTGTEEIKKIYTALDYNPETRVYGPNKKPIQWIRVHNLPDHVYFNH
                     SQHVKVGKIECAQCHGAVDSMAVVKQNSPLTMGWCIDCHRQTEVDYKDNKYYEELHKQ
                     FVNDPAYKKGDKFTIGKIGGLECSKCHY"
     gene            2604..3092
                     /locus_tag="PK281_10705"
     CDS             2604..3092
                     /locus_tag="PK281_10705"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOY49856.1"
                     /translation="MNIRFESFIKISYLIIICTVFFSFQIKAQTNDSVNTVTLNNHSI
                     LNDTLNKGSVKYNLPNGFIDLIESYKKANYASPGIDGFRVQILSDAGNNAKERAQSSL
                     VEFEQKFPGIPVYLTYQQPNFKVRCGDFRTKAEARRLLNEVSSQFPGSYVVRDFIKTP
                     KD"
     gene            complement(3089..>3532)
                     /locus_tag="PK281_10710"
     CDS             complement(3089..>3532)
                     /locus_tag="PK281_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016419786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HOY49857.1"
                     /translation="IIGFQVRPSASARKLAENEEIDMRFYSIIYKAIEEIKSAMEGML
                     APEFEEKIVCNVEVRDVFKITKVGTVAGCYVLDGKITRNTKIRLIRDGIVVYSGDLGS
                     LKRFKDDVKEVATGYECGLNIHNFNDIKEGDIIEGYENVEIKRKL"
BASE COUNT         1052 a          678 c          634 g         1168 t
ORIGIN      
        1 aagttctttt gctaactccg caatagctgc atctttagca tctaagcttt tcgaaggaag
       61 actacttcca gttagtttgt tgtaaagatt tacaacagca agtcctaatt gagaaggttt
      121 taatgcaata cgtttatcag cgtttgaacc tgttacggtc aaaagtgatt caatttgaac
      181 gtgacgagac atttttttct cgtctttatt caatttgcgt gtttcagcat attgacgagc
      241 atgttcaact gggctcagcc aatttgcaag gaaatcacat ccaattccaa caataaaatc
      301 agctttatcg aagttgtatg ttgggatgaa tgattttccg aaagaagcta agtttgaatc
      361 agcaatacct ttatacgaaa ttgaatcgta agtaacaact tgggttgttg ggtatttagt
      421 tgtgaattca gcaattgctg atttcatgga agggctgatc aaagacgaag ttaatatacg
      481 aattgctcca ccagcagctg caatgctagc tagttttgaa gttacttctg catcaacagc
      541 tttccaatca ctgtctttat tgtttagcat tgcaccttta atacgggcgc tatcgtaaag
      601 tgaaagtaca gaggcctgca cacgagcatg tgaacctcct ttagtaatag aacaaagctc
      661 gttaccatca accttgatag gacgaccttc acgtgtctta actaagacac ctgcataatc
      721 attaccatcc caataagtgg atgcataata attagcaacg cctggagtaa tttcttctgg
      781 tttattaaga taaggaattg ccttgcgaac aggagtttca catgcagcta atgaagcagc
      841 agtgacacta aatcctaaaa acttaagaaa gtctctacgg tttgttgatg aatccacgat
      901 gcgctcgtca ccgaggaatt gatcaaccgg aaggtcttct gcaaactcat tttgctgaag
      961 ttttacaaac tcaggggttg cctgaagttc ttctattcct ttccagtatt tcttcgaatt
     1021 gctcatactt cgtaaaagtc cggtttatga aggaggatgt ggatcaataa tgacatttag
     1081 aacattccaa accaccgatt ttaccaatcg tgaatttgtc gccttttttg tatgctgggt
     1141 cgtttacaaa ttgcttatgt agctcttcgt aatacttgtt gtccttgtaa tcaacttccg
     1201 tttgacggtg acaatcaata caccatccca tagttagtgg agaattttgt tttacaactg
     1261 ccatcgaatc tactgctcca tggcattgtg cacattcaat tttaccaact tttacgtgct
     1321 ggctatgatt gaaatagacg tgatcaggta gattatgaac tcgaatccat tgaataggct
     1381 tcttgttcgg gccatataca cgtgtttctg gattgtaatc tagagcggtg tatattttct
     1441 taatttcttc tgttccagtt gttgctcctt gttgaatgta tgcatgacaa ttcatacaaa
     1501 cattagctga ggggatatta gcatgcttag atttctcaac accactatgg caataaacac
     1561 atgcaatttt gttttgacca gcatggatct cgtgagaaaa tttgattggt tgttctggtg
     1621 catatccttg gtaaacacca atgcccatta gtccatccca tccttgttta agaacccagc
     1681 taactaagag aagactagct atagctacta tttttttgtt ttgagatgcc cagaaacgga
     1741 aacgttcaat tggacctaat acaggtttag gctcaatatg aataccgttt ttctcattta
     1801 ctaatgtttc cagagatgcc tttacagttg aaagaacatt aatgagaaca ataagtaaaa
     1861 cacctagtcc taataataca tagaagtatg tttcattgct agctggtttt gcctcagtta
     1921 ttgaggtccc tgctgcattt ccatcagttg gagttgcagc tacaggagca tctcctcctt
     1981 tatcagccca attgataact gctagaattt cagcatcaga taaggcttga gcaggcattg
     2041 gagaattgcc ataatcagca aaaagcttat ttgcataagc atcacctgtt ttaagaaact
     2101 ccgcagagtt cttaatccaa gaaattagtt ttgcttcatc tgtccaacgg cttttaacgc
     2161 ctttaagacc tggaccaact aatttctgat cagttggttt gtggcaggat gcgcagttgc
     2221 ctttaaacaa ctttgctcca tcttgagcat aagttgtatt agaacttaga gcaacagcaa
     2281 aaaagactgc cagaacacgt aagatatggg aacggaatag atgcatagac cagcaggtag
     2341 gatgacccct atttcagttt taacggctgc aaatttaacg gttaaccctt tcctttaaga
     2401 ttttttttga gtttgttgat aatttggaac gattctaaat aaatgaaaag ttgtgcctct
     2461 tttgaattgt attccttttg tccaataatt cagcgactta aggttgtgaa attttgtaaa
     2521 gtcatttgtt gagtccataa tattaacaaa aacataggct tatttgtgta aaaaacctga
     2581 ttagttttgc tgaaaaaaag caaatgaaca ttcgatttga atcatttatt aaaatttcct
     2641 acctgattat aatctgtacc gtcttttttt cattccagat aaaagctcaa acgaacgact
     2701 ccgttaatac tgttacatta aataatcact caattctaaa tgacacactt aataaaggtt
     2761 ctgtaaaata taatttacct aatggcttta tagatttaat agaatcgtat aaaaaggcaa
     2821 attatgcatc tcccggaatc gatgggtttc gcgttcaaat tttatccgat gctggtaata
     2881 atgctaagga aagagctcaa tcatcattag ttgagtttga acagaagttt ccaggtattc
     2941 cggtgtatct aacttatcag cagcctaatt ttaaagttag atgtggagat tttcgtacaa
     3001 aagcagaagc ccgaagactg ctaaacgaag tgtctagtca atttccgggc tcttatgttg
     3061 ttcgagattt tattaaaact ccgaaagatt aaagttttct cttaatctct acattttcat
     3121 atccttcaat gatatcacct tctttgatat cgttgaaatt gtgaatgttt aatccacatt
     3181 cgtatccagt tgcaacttct ttcacatcat ccttgaatcg ttttaaagat ccaagatctc
     3241 ctgagtaaac aacgattcca tcacgaataa gtcggatttt agtgttccga gttatttttc
     3301 catcaagcac ataacaacct gcgacagttc caactttcgt gattttaaat acatcacgaa
     3361 cttcaacatt acaaacgatt ttctcttcaa attcaggagc tagcattcct tccatcgcag
     3421 atttgatttc ctcaattgcc ttgtaaataa ttgagtagaa tcgcatgtcg atttcttcgt
     3481 tctcagctaa ttttcttgca cttgctgatg gtcgaacttg gaatcctata at
//
LOCUS       DAOOQW010000244         3416 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_33120_length_3416_cov_4.696816, whole genome shotgun sequence.
ACCESSION   DAOOQW010000244 DAOOQW010000000
VERSION     DAOOQW010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3416
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33120_length_3416_cov_4.696816"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="PK281_10715"
     CDS             <1..303
                     /locus_tag="PK281_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49858.1"
                     /translation="GIPSSKIYEYLGIGRPVLVCPGDDDILDETFSAYNQGHIANTTE
                     EAYQTLQNAFDTYLQGKYSELKADRSYTTKFARSRQAGVLAKLLNEASEKSGHSGQ"
     gene            complement(274..573)
                     /locus_tag="PK281_10720"
     CDS             complement(274..573)
                     /locus_tag="PK281_10720"
                     /inference="COORDINATES: protein motif:HMM:NF014616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HOY49859.1"
                     /translation="MLDKLMEMKKRMDEVKARLDAITVTGDADQGKVKIHMNGNKKVT
                     AVNIAPELLDTGDSEQLEDLIQVAVNRALEQAERVNESEMRSVAGGLLPGMPGLF"
     gene            655..924
                     /locus_tag="PK281_10725"
     CDS             655..924
                     /locus_tag="PK281_10725"
                     /inference="COORDINATES: protein motif:HMM:NF019246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkoF family
                     thiamine/hydroxymethylpyrimidine-binding protein"
                     /protein_id="HOY49860.1"
                     /translation="MEATIDISLYPLDEKYAAIITDFILKLKDDTDLRVEVNGLSTQI
                     FGSYDYLFEVLQTTLKPVLMAYPAVAVLKIAAGTHTKEQLPSTLK"
     gene            complement(1654..1929)
                     /locus_tag="PK281_10730"
     CDS             complement(1654..1929)
                     /locus_tag="PK281_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49861.1"
                     /translation="MFKKDNFIFGALLGAVIPMVIYLILENFVTITLNTGKTEPRFDQ
                     PTMLVLALVGNILPFRQYMQKGYYEKTGKGILLMTFVYAVVYVYLKF"
     gene            complement(1922..2692)
                     /gene="surE"
                     /locus_tag="PK281_10735"
     CDS             complement(1922..2692)
                     /gene="surE"
                     /locus_tag="PK281_10735"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008270508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HOY49862.1"
                     /translation="MSERKLILVCNDDGITAPGIRALVAVAKEFGDVVVVAPDKPQSA
                     MGHAITINATIRVNPVKVDGVIEYACSGTPVDCVKLAVNKILHRKPDLLVSGINHGSN
                     ASINVIYSGTMSAAMEGAMEQIPSIGFSLLDHSIDADFSAAQDVARILIAQALENEMP
                     DRICLNVNIPNVSKELIKGIKVCRQAIANWEEEFEERKDPSGKPYFWLTGIFKNYDSG
                     TDTDIWALENNYVSVVPCQFDITAHTTLNHFKSWEQHV"
     gene            complement(2819..3301)
                     /locus_tag="PK281_10740"
     CDS             complement(2819..3301)
                     /locus_tag="PK281_10740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49863.1"
                     /translation="MSKVIVLSGHGSWEMKDGYVTLPAKCSIKFYTCNMKTLSDGLGG
                     DIDRGIVSGLDPDQEAGPFSTVPNMRLYPPAGLLIRTPDARTWNVVDLPGPVPHDNKN
                     LQVRIANQYGGGGDLKTLLDMLRPAITGQEVTILWAACRAIGLKNAGGKSIGINTMQR
                     "
BASE COUNT         1086 a          690 c          672 g          968 t
ORIGIN      
        1 ggaattcctt ctagtaagat ctacgaatac cttggaattg gtcgtcccgt tttggtttgt
       61 ccgggtgatg atgatattct cgatgaaacg ttttcggctt ataaccaggg acatattgcc
      121 aatacaactg aagaagctta tcagacttta caaaacgcat tcgatacgta tctacaaggc
      181 aagtattctg agctcaaggc cgacagatcg tacacaacga agtttgcccg atcacgtcag
      241 gcgggcgttt tggcgaaatt actaaatgaa gcctcagaaa agtccgggca ttccgggcaa
      301 taaaccacca gcaacagagc gcatttctga ctcattcaca cgttcagctt gttcgagtgc
      361 acggttcacg gcaacttgaa ttaggtcttc gagttgttcc gaatcgccag tatcgagtag
      421 ttcgggagca atattcacgg cagtaacttt cttgttaccg ttcatgtgaa ttttgacttt
      481 cccctgatcg gcatcgcccg ttacagttat cgcatccaaa cgagctttca cttcgtccat
      541 ccgctttttc atttccatga gtttgtcgag catagctgag attttgctgc aaaactaatc
      601 aaaccgaaaa agaaaagtgc gtagaattta ggtggatatt tgcaaaaaac acacatggaa
      661 gcaacgattg atatcagctt gtatcctttg gatgaaaaat atgcagcaat cattaccgac
      721 ttcattctaa aactcaagga tgataccgat ttgcgggttg aagtaaatgg attgagtact
      781 caaattttcg ggagctatga ttacttattc gaagtacttc aaaccacgtt aaagccagta
      841 ttgatggcct atccagctgt agcggtttta aaaattgctg ctggaacaca tacaaaagag
      901 caattaccaa gtactttgaa gtagttttcc gaaaaacgac attttaatcg agttaaaaac
      961 tcgaggttat taaccaatcc acttattaac agtcagttaa taaacccaat aacattcaaa
     1021 caaaattagg ttttaagggg cttacatgaa agcccacaag ttgataacct ggtgcatatc
     1081 gagttcaaaa ctacacctcg atttttcttg cttagttcct gattttgtgc acctttgata
     1141 cagcagatga ctgcaacgtt ctttaacgca taggaaattc agtttactga aaatctcaaa
     1201 aaaacagcaa tgaaaattgc tgcacgatga aaaccaaaag ttaagatcac cacgtgattc
     1261 ctcttttgga gaagaaggca accgattctt tcatctatgt gatttcggga agaatgtttt
     1321 aaaatggaca gatagagcgc caatctcaaa tcaaatgtgt tgaaatacat atttgactag
     1381 ttcttggaag agcccatcaa caagttttaa atacattgat aatctgttcc gattaattgt
     1441 ttgaaacagc aatttaaaaa gacagaggag aacgaccaca gcacaaacca ggctcttttg
     1501 agagtcgtgc gattaaagat agtagttcaa aagccaaact gatttgttac ggcaaagaag
     1561 tttgaaaaag ggaattctgg tcaaacagga ttcccttttt ttatgtgttt tttttgagaa
     1621 gaatagaaat cagccagact taagttcttc gatttagaac ttcaaataca cataaaccac
     1681 agcatatacg aaagtcatga gaagaatacc ttttcctgtt ttctcgtaat atcctttttg
     1741 catgtattga cgaaaaggaa ggatatttcc cactaaagcc aaaacaagca tggtaggttg
     1801 atcaaaacga ggttcggttt tgcccgtatt aagggtaatg gttacgaaat tttcgagaat
     1861 gaggtaaatc accatgggaa tgacggctcc cagcaatgct ccgaaaatga aattgtcttt
     1921 cttaaacatg ttgctcccaa cttttgaaat ggttcaaagt agtatgagca gtaatatcga
     1981 actgacaagg cacaaccgac acgtagttat tttctaaggc ccaaatatcg gtatcggtgc
     2041 ctgaatcata atttttgaag attccggtta gccagaaata cggttttccc gatggatctt
     2101 ttcgctcttc gaattcctct tcccagttgg caattgcttg tcggcacact ttaattccct
     2161 tgataagttc tttactcaca ttgggaatat tgacattgag acatatacga tcgggcattt
     2221 cgttttcgag cgcttgagcg atgagaatac gagctacatc ttgagcagcc gaaaaatctg
     2281 catcaatgga atgatcgagt aaagagaaac cgatagaagg aatttgctcc atagcacctt
     2341 ccatagcagc cgacatggtt ccagaataaa tcacgttaat agaagcattc gagccgtggt
     2401 tgattccaga gaccaataaa tcgggtttgc gatgaagtat cttgttaacc gccaacttca
     2461 cgcaatcgac aggagtgcca gaacaagcat attcaatcac cccatcaacc ttaactggat
     2521 ttacgcgaat agtcgcattg attgtaattg catgtcccat tgccgattgc ggtttatcgg
     2581 gtgctacaac aactacatct ccaaattcct tcgcaacagc aaccaaggct ctaatgcccg
     2641 gagcggtaat gccatcatcg ttacaaacca gaatcaactt cctttcggac ataatcaaaa
     2701 ttttgcagcc aaattaggca ttttggcaaa gggagtagaa gaatttcctt cggaaaaagg
     2761 cataaaaaaa cgatgtactc gaatgaatac atcgttctca ataagagtat tgtattgatt
     2821 atcgttgcat ggtattaata ccaatcgatt ttccacctgc gtttttcaat ccaattgcgc
     2881 ggcaagcagc ccaaagaatg gtaacttctt gtccggttat agcaggtcgg agcatatcaa
     2941 gtaaagtttt caaatcacca ccaccaccat attgattggc aatacgaact tgtaggtttt
     3001 tattgtcgtg gggcacagga ccgggtaaat caactacatt ccaagtacgt gcatcgggag
     3061 tacgaattaa caatccagca ggtggatata aacgcatatt tggtacagtt gagaatggac
     3121 cagcttcttg atccggatct aaaccggata caattcctcg gtcgatatct ccacctaagc
     3181 catcgctaag ggtcttcata ttacacgtat aaaacttaat gctgcatttg gctggcagag
     3241 taacatagcc atctttcatt tcccaagatc catggccact taaaacgata acttttgaca
     3301 tacttagatg aaaattaatg gtaaaacaag gagtaaattg acctatcggt attttcaaat
     3361 ataaatatat ctaccagaat atagggtgat taaggaacta tgtgataaaa taatta
//
LOCUS       DAOOQW010000245         3414 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_33151_length_3414_cov_5.027985, whole genome shotgun sequence.
ACCESSION   DAOOQW010000245 DAOOQW010000000
VERSION     DAOOQW010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3414
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33151_length_3414_cov_5.027985"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            28..855
                     /locus_tag="PK281_10745"
     CDS             28..855
                     /locus_tag="PK281_10745"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOY49864.1"
                     /translation="MKVLVRLFVLGFACITSFLNAQVAPVYTDPLNVITSKDPVLIQA
                     IEFLRYGDSEGALKILLKNKTGSENSYESYFLKGIAYRQTGDLTNAIHAFTQSVHEDG
                     FFIPGFFERGNCYLYRQNFGLAVFDYDRVLLLDSTFLPAYNNRAYARIRNYGEQGNPT
                     QQLRFAKEDLNKIIEISSVRGDKPRFEYFFNIGLIDLYSSEYKLASESFSRAIQVDPS
                     ISKAYYFRGASLFLAHSYELAAVDFRTSEEMGFVHANTPEFLRIIDLVEQYQQEHKE"
     gene            852..1985
                     /locus_tag="PK281_10750"
     CDS             852..1985
                     /locus_tag="PK281_10750"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HOY49865.1"
                     /translation="MSQGKFPSKLVDRLRDREQSGLFRSLKQTDDLIDFCSNDYLGLS
                     SERSIHERVSKLISEQGTRFNGSTGSRLIRGNTYQAEELEAWLAQFHRSEAALIFNSG
                     FDANLGIFSSVPQRDDTILYDKLIHASIHDGIRLSYAKSYSFKHNDVEDVRRLIRQST
                     GQVYIAVESLYSMDGDIAPLADLASLCKETGALLIIDEAHATGVFGPEGRGLAIEDDI
                     LEQCIARIYTFGKAIGAKGAVVVGAFSLKAFLINFARSFIYTTALPIYDLLSVQAAYD
                     KLLKEPERRVSLFERIKLYKDLMKSLPDLDFIQADGPIQGVIFGSNQEAIRVSRLMQE
                     SGLDVRPILSPTVPVETERIRICIHSFNQVNDMHRLFEVLRNS"
     gene            2064..2222
                     /gene="rpmH"
                     /locus_tag="PK281_10755"
     CDS             2064..2222
                     /gene="rpmH"
                     /locus_tag="PK281_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015755253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HOY49866.1"
                     /translation="MVRTYQPSVRKRRNKHGFRERMSSANGRRVLAARRARGRKRLTV
                     SSDKLHKR"
     gene            complement(2266..3069)
                     /locus_tag="PK281_10760"
     CDS             complement(2266..3069)
                     /locus_tag="PK281_10760"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOY49867.1"
                     /translation="MEFQAGDIQWKATRFGTGKTWLIALHGYGQQASLFHHLAEICKD
                     KYSILAIDLAYHGNNDSFQKGFLFDKNYAQVWLDSILEKTGQKQIGLVGYSIGGRISL
                     SLTSWFPELISELILLAPDGVPVSTLYRLLTNNAIGNALFRTFIHSPGFAFGLIKLGV
                     WLKIIPTKVSDFYRNEISTLQKRQQLYDAWMAYRLAIPQYDIIKKYLKKGKISVVCIL
                     GRNDKVIPFTKTRKAALKFLPGIQIIELEAGHNLLSDKATKLLAPYWRA"
     gene            3161..>3414
                     /locus_tag="PK281_10765"
     CDS             3161..>3414
                     /locus_tag="PK281_10765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49868.1"
                     /translation="METLLTLFYLILFLFIVRFWKGFYLPGLPNYSAVVIYLIKFAAG
                     VALAWVYKEYYTDRSTADMYKLFDDSKFMYEALSDKPADF"
BASE COUNT          983 a          587 c          719 g         1125 t
ORIGIN      
        1 gttgattaat acaacaaatt aaggtgtatg aaagtgctag ttcgactttt cgttttaggt
       61 tttgcatgca tcactagttt tttgaatgct caagttgccc ctgtatatac tgatccttta
      121 aatgttatta cttcaaaaga tccggtatta attcaagcga ttgaattttt gagatacgga
      181 gattcggaag gtgcattaaa gattttattg aagaataaaa cagggtcgga aaactcgtat
      241 gaatcgtatt tcctaaaagg aattgcctat cgtcaaactg gtgatttgac taatgctatt
      301 cacgcattca cacaatcagt acatgaggat gggtttttta ttcctggatt ttttgaacga
      361 gggaattgct atttgtacag acagaatttt ggactagcag tatttgatta cgatcgggtt
      421 ttattgttag atagtacatt tttacctgct tataataatc gagcttatgc ccgaatccga
      481 aactatggag agcaaggaaa tcctacacaa cagttgcgat ttgcaaaaga ggatttgaat
      541 aagatcatcg aaatttcatc tgtccgtgga gacaaaccaa ggtttgagta tttctttaat
      601 attggattga tagatcttta ttcatcggaa tataaactgg catcagagag ttttagcaga
      661 gctattcaag tagatccatc tatttccaaa gcttattatt ttcgaggagc ttcattattc
      721 ttagctcatt cttatgaatt ggctgccgta gattttagaa cttccgagga gatgggtttt
      781 gttcatgcaa atactcctga gtttttaagg attatcgatt tggttgaaca atatcaacaa
      841 gaacataaag aatgagtcaa gggaaattcc ccagcaaact tgttgataga ttaagagata
      901 gggaacaatc gggtctattc cggagcttaa agcaaacgga tgatttgatt gatttttgtt
      961 cgaatgatta tctaggactt tcttccgagc ggtcgattca cgaacgtgta agcaaactta
     1021 tttcagagca aggaacccga tttaatggat caacaggatc gcgtttaatt cgtggaaata
     1081 catatcaagc ggaagaattg gaagcttggc tggctcaatt tcatcgttca gaagctgcct
     1141 tgatattcaa ttcaggattc gatgccaatt tgggaatttt ctctagtgtg cctcagcgtg
     1201 atgatacgat cttgtatgac aagttgattc atgcttcaat tcatgatggt attaggttgt
     1261 cgtatgctaa gtcatattcc tttaagcaca acgatgtaga agatgtgagg aggctaattc
     1321 gccaatcaac tggacaagtt tatatagctg tagaaagttt gtattcgatg gatggagata
     1381 ttgctcctct tgccgattta gcgtctttat gtaaagaaac tggtgcattg ttgattattg
     1441 atgaagccca tgctacaggt gttttcggcc cagaaggcag aggtttggca attgaggatg
     1501 atatccttga acaatgtata gctcggattt acacatttgg taaagctatc ggggctaaag
     1561 gagctgttgt agttggggca ttttcgctca aagcttttct catcaatttc gcccgatcat
     1621 ttatctatac aacggcttta cctatttatg atttgttaag tgttcaagca gcttatgata
     1681 aattactgaa agaaccagag cgccgagtaa gtctgtttga gcgaataaag ttgtataaag
     1741 atttaatgaa atcgctgcca gatttagatt ttattcaagc agatggtcca atacaaggag
     1801 ttattttcgg ttcgaatcaa gaagcaattc gagtttctcg attgatgcag gaaagtggat
     1861 tggatgtaag accgatactt tctccgacag tgccggttga aactgagcgg attagaattt
     1921 gcatacatag ctttaatcaa gtgaatgaca tgcatcgttt gtttgaagtt ttacgtaatt
     1981 cttagatgct tgatattttt ttagcataaa cgtttggaga tttcaaaaaa cgtcctactt
     2041 ttgcgttccc aaattttaga gccatggtaa gaacgtatca gccctcggtc cggaagagaa
     2101 gaaacaaaca cgggttccgt gaacgcatgt cgtccgctaa tggtcgtcgt gtattggctg
     2161 cacgtcgtgc ccgcggcaga aaacgtttaa cagtgtctag cgacaagctg cacaaacgtt
     2221 aatctgaacc gatatttatc cggaagcggc cttgtgccgc tttttttatg ccctccaata
     2281 aggtgctagg agttttgttg ctttgtcgct cagtaaattg tgaccggctt caagttcaat
     2341 aatttgaatc ccaggtagaa acttaagtgc tgctttacgt gtcttggtaa acggaatgac
     2401 tttatcgttt cttccaagaa tacacactac tgagattttt ccttttttca aatacttctt
     2461 gataatatca tactgaggaa tcgcaagcct gtatgccatc cacgcatcat ataattgttg
     2521 tcttttttgg agtgtgctga tttcgtttcg ataaaagtca gagacttttg tggggataat
     2581 ttttagccat actcccagtt ttattaatcc aaatgcaaag cctggtgaat gtataaaagt
     2641 tcggaacaac gcatttccta tggcattgtt ggtgagaagc ctatacagtg ttgaaacagg
     2701 aacaccatcg ggcgctaaca gaattaattc gcttatgagt tcgggaaacc aactagtaag
     2761 actaagtgag attctgcctc caattgagta gccaacgaga cctatttgtt tttgtcctgt
     2821 cttttctaaa atggaatcca accaaacctg agcgtaattc ttatcgaata gaaatccttt
     2881 ttggaaagag tcattgtttc catgataggc taagtcgatg gctaaaatgc tgtatttgtc
     2941 tttgcaaatt tctgctaggt gatggaaaag cgaagcttgt tgtccatacc catgtaaagc
     3001 aatcaaccat gtttttcctg tgccgaagcg agttgctttc cattgaatat ccccggcttg
     3061 aaattccata gcatgaatta aaaaatgaaa gtagtttaat ctctaatcat ttcaaattct
     3121 acaaacctta gactgttttt gaattaatac ctttgaaggc ttggaaacgc tactaaccct
     3181 tttttacctg attttattcc tgttcatagt caggttctgg aaaggatttt atttgcctgg
     3241 tcttccaaat tattcagctg ttgtaattta tttaatcaag tttgcggcag gtgtagcgtt
     3301 agcttgggtt tataaagagt attataccga tcgttcaaca gccgatatgt ataagctctt
     3361 cgatgattcg aaatttatgt acgaggcatt atcagataag ccagctgatt tttt
//
LOCUS       DAOOQW010000246         3403 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_33283_length_3403_cov_6.228495, whole genome shotgun sequence.
ACCESSION   DAOOQW010000246 DAOOQW010000000
VERSION     DAOOQW010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3403
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33283_length_3403_cov_6.228495"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..793)
                     /gene="hemC"
                     /locus_tag="PK281_10770"
     CDS             complement(<1..793)
                     /gene="hemC"
                     /locus_tag="PK281_10770"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HOY49869.1"
                     /translation="MARKICIGSRGSDLALWQAHFTKKQLEDLGHSVEITIIKTKGDQ
                     IQHLSFDKIEGKGFFTKELEDALLQGNIDLAVHSHKDLPTNFPPGLTISGVSYREDCS
                     ESILIRPEAYQSTLPLGLRMNARVGTSSFRRRSQLLAIRPDLEITDLRGNVPTRVDKL
                     RQGNYDAIVLASAGLNRLNLDLSDLQRQILPPHFFVPAPAQGVLAFQTRESDIEMRDI
                     IAQIHHTNVQDAIFCERSVLNQMQGGCLLPLGVFCQFTGTDYQVWA"
     gene            complement(796..2022)
                     /gene="hemA"
                     /locus_tag="PK281_10775"
     CDS             complement(796..2022)
                     /gene="hemA"
                     /locus_tag="PK281_10775"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HOY49870.1"
                     /translation="MKEFKVIAFTHRQTPLDQVGRYHLVPEERAARLSVLKQELKLDE
                     LLYLSTCNRVEFVFIYSGKADSSFLLHFFQVLFPGWTKEDSTLMLNQSQVMEGDEAIR
                     HVFHVAASLDSLVVGEREIITQIRSAHEDSAREKLSGDSIRLLMRKVIESAKEVFTDT
                     PIARNPVSVVSLAYRHLKSLQVKTEARFIIIGAGQTNNNLCKYLHKHGFRNFTVFNRS
                     FDKAQELAANIGGIALPLEDLSSFNSGFDVLVACTAASDHLVSPALYAHLLQGDTDRK
                     VVIDLAIPYDVDPHIYKEFNVTPILVESLRQAAEQNLALRHSALFQCEEIIDKHCTEF
                     RDMFRERQIERAMQEVPQKVREIREFATNSVFARELENMDDASREVLEKVLTYMEKKY
                     ISVPMKMAKEIMLEKH"
     gene            2405..2920
                     /locus_tag="PK281_10780"
     CDS             2405..2920
                     /locus_tag="PK281_10780"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOY49871.1"
                     /translation="MKKQAERLRLIRVSKSMSQENVAKALDITVGAYSKIERGVTKLS
                     LNRLADLAKIFDIELNDFIRYLNGEQDSLDRKLNIPGAGSTSNFGSSNTAVDSEVALL
                     RKIINLYDESKELNTKAVVRNMFENNNVNIGNFLDVLKDCSTNLASRKLDGNQITNYN
                     KGIERIVELLS"
     gene            3009..>3403
                     /locus_tag="PK281_10785"
     CDS             3009..>3403
                     /locus_tag="PK281_10785"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOY49872.1"
                     /translation="MHKISAVIITFNEERNISRCLDSLQGIVDEIVVVDSYSTDRTQE
                     LVENSGARFIQHTFEGHIEQKNYALSQASHDWVLSLDADEALSDQLKTAIRQVKTSIN
                     HDGYTMNRLSNYCGHWIHHGSWYPDTKLRL"
BASE COUNT         1074 a          669 c          694 g          966 t
ORIGIN      
        1 aagcccatac ttggtaatcg gttccggtaa actgacaaaa tactcctaat ggcaacaagc
       61 atccaccttg catttggttc aggacgctac gttcgcagaa aatagcatct tgcacattag
      121 tgtgatgaat ttgagcaata atatcccgca tttcgatatc cgattctcgg gtttgaaaag
      181 caagaacacc ttgagcagga gccggaacga aaaaatgtgg aggaagaatt tgtctttgca
      241 aatcgctcaa atctaaattc aagcgattga gaccagcaga tgccaagaca atagcatcgt
      301 aatttccctg acgaagttta tcgacacgag taggtacatt accacgtaaa tcggtaattt
      361 ctaaatcggg gcgaatggct agtaattgag aacgacgtcg aaaagaggat gtgccaacac
      421 gtgcattcat tctgagccca agaggcaagg ttgattgata tgcttcgggg cggataagaa
      481 tagattcgga gcaatcttcg cgataggaaa cacctgaaat ggtgagtccg ggaggaaaat
      541 tagtagggag atctttatgg gaatgtacgg cgagatcgat attgccttga aggagagcgt
      601 cttcgagttc tttggtgaag aaaccttttc cttctatttt atcgaaagaa agatgttgaa
      661 tttgatcccc tttggttttg atgatggtga tttcgactga atggccgaga tcttcgagtt
      721 gttttttggt gaagtgtgct tgccaaaggg ctaaatcact tcctctggaa ccgatacaaa
      781 tttttcgagc catgactaat gcttctccag cataatttcc tttgccattt tcatgggaac
      841 gctgatgtat tttttctcca tatatgtaag cactttttca agcacttcgc gggaagcgtc
      901 atccatgttt tctaattccc gtgcaaatac cgaattggta gcaaattctc ttatttcgcg
      961 aactttctga ggaacttcct gcatagcacg ttctatttga cgttcacgga acatatcgcg
     1021 aaattcggtg caatgcttat cgataatctc ttcgcactga aataaggcag aatggcgtaa
     1081 tgctaagttt tgttcagctg cttggcgaag gctttctact aagataggag tcacgttaaa
     1141 ttccttgtag atatgaggat caacatcata aggaatagct aaatcgatta caacttttcg
     1201 atcggtgtcg ccttgcaata aatgtgcgta tagagcaggg ctaaccaaat ggtcagaagc
     1261 agcggtgcaa gcaacaagca catcaaaacc tgaattgaaa gaacttaaat cttccaatgg
     1321 taaagcaatg ccaccaatat tagcagcaag ttcttgagct ttatcgaaac tccggttaaa
     1381 taccgtaaaa tttctaaaac catgcttatg caagtactta cagagattgt tgtttgtttg
     1441 gcccgcgcca ataatgataa acctagcttc agttttaacc tgaagagatt tcaaatgacg
     1501 ataggccaac gaaacaaccg aaactggatt gcgagcaatg ggagtatcgg taaaaacctc
     1561 tttggccgac tctatcactt tacgcatcag caaacgaata gaatccccgc ttaatttttc
     1621 tcgtgcagaa tcttcgtgtg cagaacgaat ttgagtaata atctcacgct cgccaacaac
     1681 caaagaatcg agcgaagctg caacatgaaa cacatgacga atggcttcat cgccttccat
     1741 cacttgtgac tgatttaaca tgagggttga atcttctttc gtccagccgg gaaataatac
     1801 ttggaagaaa tgaagtaaaa atgacgaatc agcctttccg ctgtatataa aaacaaattc
     1861 tacgcgatta cacgtactca aatagagcaa ctcatcaagc tttagctctt gcttcaagac
     1921 agaaaggcgt gctgctcttt cctcaggaac aaggtgataa cgcccaactt gatcaagtgg
     1981 cgtttgacga tgagtaaatg cgatgacttt gaattccttc aaaacacaat tctatgcaac
     2041 aaaagtacga gaggtggctc cgaagaatgt cattgaattg tcaaagcaag ccacagcagc
     2101 ctatttaaac taattccaaa taagccagat tgaaaatgtc aaataattgt ctgtataata
     2161 tttacgcatc caaagttcaa ccaaaaataa ccaaatttga ataataccta tggggtattt
     2221 cagctacaaa tcaccattaa aagaaaacta aggcttaaca tatttaattg atttcgtgcg
     2281 aattgggaaa tcagcagttt taattagatt tatttgtgac ttttttttct cttttgtgaa
     2341 tgaaacttaa gtcttaagaa ctcaaaattt gtagttttgt cgttcaacca tctacaacac
     2401 accgatgaaa aaacaagcag aacgactcag actaattcgg gtttcgaaga gtatgagtca
     2461 agaaaatgtg gctaaagctt tagatattac cgttggagct tattctaaaa ttgagcgcgg
     2521 ggtaacgaaa cttagcttga accgtttagc tgatttggct aaaatttttg acattgaact
     2581 caatgatttt atccgctacc taaatggtga gcaggatagc cttgatcgta aattgaatat
     2641 tcctggagct ggcagcacat caaattttgg cagttctaat acagcagtag attctgaagt
     2701 tgctttgctt cgtaaaatca tcaacctata tgatgaaagt aaggagctta acaccaaagc
     2761 ggtagttcgc aatatgtttg aaaacaacaa tgtgaatatt ggtaacttcc ttgatgtatt
     2821 aaaagattgc tcaaccaatt tggcatcccg caaattagat ggaaaccaaa ttaccaacta
     2881 caataaaggc attgagcgca ttgtagaact tttaagctaa gaataatcct catttttaaa
     2941 ggtctgtcgg gttgctggca gacctttttt attttggtca agttcaattt ttagtacgtt
     3001 tgaacaccat gcacaaaatc tcggcagtca tcatcacctt taacgaagaa cgaaacatca
     3061 gtagatgtct agattctctt caaggtattg tcgatgaaat tgttgtagtg gattcctact
     3121 ccaccgaccg aacacaggaa ttagtagaga attccggtgc tcgattcatt caacatacct
     3181 tcgaaggtca cattgagcag aagaattacg ctctttctca agcaagtcac gattgggtgc
     3241 tttcgttaga tgcagatgaa gcactttccg atcaactcaa aactgccatc cgccaagtga
     3301 aaacctcgat taatcacgac ggttatacga tgaatcgctt gagtaattac tgcggacatt
     3361 ggattcatca tggaagttgg tatcccgata cgaaactgag gct
//
LOCUS       DAOOQW010000247         3403 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_33285_length_3403_cov_5.453704, whole genome shotgun sequence.
ACCESSION   DAOOQW010000247 DAOOQW010000000
VERSION     DAOOQW010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3403
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33285_length_3403_cov_5.453704"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            148..753
                     /gene="yihA"
                     /locus_tag="PK281_10790"
     CDS             148..753
                     /gene="yihA"
                     /locus_tag="PK281_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007095057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HOY49873.1"
                     /translation="MEISTAEFVISNSDVEKCPAPRLPEYAFIGRSNVGKSSLINMLT
                     RRAKLAKTSGRPGKTQLINHFLINKNWFLVDLPGYGFARASKEQKSVFGQIITDYIAN
                     RTNLMNLFVLIDIRLEPQAIDLNFFRWLGTNGIPFVIVFTKSDKLSHNQLQSSLARYK
                     RTLQEEWEVFPTHFVTSSETSLGRDEILKFISQTNTLFTSY"
     gene            complement(750..2450)
                     /locus_tag="PK281_10795"
     CDS             complement(750..2450)
                     /locus_tag="PK281_10795"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOY49874.1"
                     /translation="MRHFCWCILLLFATKSGAQTLCQVIGAEVYAGPGLQVSINGSIA
                     LDSQAHFQNFSELHISGDLINKGQLEHSGSVFIQGNWVNDSIFQANPISLVEFNGAAQ
                     QIEGTHVCRFGQLKLSGTGDKNLLQTAEAEELDLSDRLLITDSSRIRVLSNDVQAIER
                     TSGYVISNRFGSLERALQTGVQYLYPVGSLSNYQPVFINPAGNSTYAGVRFAAVDAST
                     EGLPRTQVDTTICRSNASFFHLFYGDLAAGSSISMNVATSDVNSFPFLAQRNPQQTGV
                     WTLVPSSTTLVSGGITAVNHTLSTAIDSRAFLLVRGRPSRPIISGDTAFCKKSLNRTF
                     TITHDSTLVTNWTLASGNIVQQNDSVLIVNWGEESNGLVSAFQSDSAGCSSFPASHSV
                     ILWDNPVANFVIDAPDLPYEDVPFTLTSLSSGAVGQYWSIANLMNYSDSVIQVVFDAP
                     GVYPVLLQVVNEFGCSDTASGIIEVIEGMVIPNTFSPNGDGINDELYLINSGVQEFNL
                     KIFDRWGVLVFETNASKLSWDGKTSSGERVPAGTYMMVLSAKTANSTFEKRGALTIFD
                     "
     gene            complement(2447..2797)
                     /gene="gldC"
                     /locus_tag="PK281_10800"
     CDS             complement(2447..2797)
                     /gene="gldC"
                     /locus_tag="PK281_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014782599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldC"
                     /protein_id="HOY49875.1"
                     /translation="MSTKNTSDIILRVTLDANKVPEKIHWDATDNQVVGKEAKAMMLS
                     VWDPTEDNTLRVDLWTKEMVVDDMKKFIHQSLLLMADTLQRSTGEIAMAETMRDFGAY
                     YAEKLGLITPEEKP"
     gene            complement(2794..>3403)
                     /locus_tag="PK281_10805"
     CDS             complement(2794..>3403)
                     /locus_tag="PK281_10805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49876.1"
                     /translation="AVSTLISGFNYNVVVSDSNLAIGLDMYLGKNCKFYELLSLPKYK
                     VSKMNRTMIVPDAIRGWLMSNFEMDASQEDLVSYMIYHGKMMYLSQQLLPDKTEWNIL
                     GYTEKELSWCADNEARIWSHFIDRKLFFSKDFNDQLIYINDGPFTKGFPEEAPSRIGI
                     WLGFQIVKSYMNKYQNITLPELINDQDAHKIFNSSGYKPGRA"
BASE COUNT         1034 a          736 c          643 g          990 t
ORIGIN      
        1 aaaaatagaa gagttggccg aagccgatgc gctctataaa aatcctaaat caacatacac
       61 gcaaagtctt attgcctcca ttcctcttgg tcgccctcag gatatagacc gtgccatcga
      121 attaagaaaa atctaatttt ccccagtatg gaaatcagca cggccgaatt tgtaattagt
      181 aatagtgatg tagaaaagtg tccggctcct cgcctaccgg aatatgcctt tattgggcgc
      241 tcgaatgtgg gcaaaagttc gctgattaac atgctaactc gaagagctaa actggctaaa
      301 acatccggtc ggccaggaaa aactcaatta ataaaccact ttctgataaa taaaaactgg
      361 tttcttgtcg atcttccagg ttatggattc gctcgtgcta gtaaagaaca aaaaagcgtt
      421 tttggtcaaa tcatcaccga ttatatcgcc aatcgaacga acttgatgaa tctgtttgtc
      481 cttatcgaca ttcgtttaga accgcaagcc atcgacctca acttttttcg ttggttaggt
      541 actaatggga ttccttttgt gatagttttt accaaaagcg acaagctttc tcacaaccaa
      601 ttacagtcga gtttagctcg ttacaaaaga actttgcagg aagaatggga agtatttccg
      661 acacacttcg ttacctcatc tgaaacctct ttaggaagag atgaaatatt aaaattcatc
      721 tcacaaacca acacactttt tacttcgtac taatcgaaga ttgtaagcgc gccgcgtttt
      781 tcgaatgtac tattagccgt cttcgcagat aataccatca tgtacgttcc tgctggaact
      841 cgttctccac tgctggtttt tccatcccaa cttaatttcg aagcattcgt ttcgaaaacg
      901 agaacacccc atcgatcaaa aatcttcaag ttaaactcct gaacaccact attaatcaaa
      961 taaagctcgt cgtttattcc atctccgtta ggtgaaaatg tattcggtat caccattcct
     1021 tcaatcactt caattattcc gctggcggta tcagaacaac caaattcatt tacaacctgt
     1081 aataaaacag ggtaaactcc aggtgcatca aataccactt gaataaccga atcagaatag
     1141 ttcatcaaat tggcaattga ccaatattgc ccaacggcgc cagaacttaa actggttaag
     1201 gtgaacggaa catcttcata aggtagatcg ggtgcatcaa taacgaaatt agcaaccgga
     1261 ttatcccaca aaatcaccga atggcttgca ggaaaagaag aacatcccgc actatccgac
     1321 tggaaggctg aaactaaacc attggattct tctccccaat taacaattaa aacactgtcg
     1381 ttttgttgta caatattgcc agatgctaat gtccaattgg tgactagggt agaatcgtgt
     1441 gtaatagtaa acgtgcggtt taaacttttt ttacaaaagg ccgtatcgcc cgatatgatt
     1501 ggtcttgaag gtcgtccgcg tacaagcaaa aaggctcgac tatcaatggc agtcgacaag
     1561 gtgtgattaa cagcagttat cccgccggat actagtgttg ttgaagacgg aactagtgtc
     1621 caaacgcctg tttgttgagg atttcgctgt gctaagaatg gaaaactatt tacatctgaa
     1681 gttgccacat tcatactaat cgaacttcct gcagctaaat caccataaaa taaatggaaa
     1741 aatgatgcat tactccgaca tatggttgta tcgacttgcg ttctaggtaa tccttctgta
     1801 cttgcatcaa ccgcagcaaa acgaacaccg gcataagtag agtttcctgc aggatttata
     1861 aaaacaggtt ggtaattcga caagctacca acgggataca gatattgaac acctgtttgt
     1921 aatgcccgtt ccaaacttcc aaatcgattc gaaattacat atccgctggt tcgttcaatc
     1981 gcctgaacat cattagaaag aactcgaata cgtgatgaat cagttattaa aagtcggtcg
     2041 cttaaatcaa gctcttctgc ttcggctgtt tgaagtaaat ttttatctcc tgtgcccgag
     2101 agcttcaatt gaccaaaacg gcaaacatgc gtgccttcaa tttgctgtgc agcaccatta
     2161 aattcaacaa gcgaaattgg attagcttga aaaatcgaat cgtttaccca gtttccctga
     2221 ataaagactg agccggaatg ctcgagttgt cctttgttta taaggtctcc agaaatatgt
     2281 aattctgaaa aattttggaa atgagcttga ctgtcgagtg caatacttcc attaatgctc
     2341 acttgcaatc ccggaccagc ataaacctct gctccaatta cttggcaaag agtctgtgcg
     2401 ccagatttag tggcaaataa taaaagaatg caccaacaga aatgcctcat ggtttttctt
     2461 cgggtgttat aagtccaagt ttttcggcat aataagcacc aaaatcacgc atggtttcgg
     2521 ccatcgcaat ttctcctgtt gagcgctgca gcgtatctgc cattagaagc aaactttggt
     2581 gaatgaattt tttcatgtcg tccacaacca tttctttcgt ccataaatca actcggagcg
     2641 tattgtcttc ggttggatcc caaacagaaa gcatcattgc ttttgcttct ttcccaacaa
     2701 cctgattgtc ggtggcgtcc caatgaattt tttctggaac tttattggca tctaatgtta
     2761 ctcgaagaat gatatctgaa gtgttttttg tgctcatgcg cgaccgggtt tgtatcctga
     2821 agagttaaaa attttatgcg catcctgatc gttgattaat tcgggaagcg tgatgttttg
     2881 atatttgttc atgtagcttt ttacaatctg aaacccaagc caaataccta ttcgggaagg
     2941 agcttcctcg ggaaatcctt ttgtaaacgg accatcattg atgtaaatca actggtcgtt
     3001 gaagtcttta gagaagaata gttttcgatc gatgaaatga ctccagattc gagcttcatt
     3061 atctgcacac caactgagtt ctttctcggt atatcctaga atattccact cggttttgtc
     3121 gggtaatagt tgctgactta aatacatcat tttgccatga taaatcatat acgaaacgag
     3181 gtcttcttgt gaggcatcca tctcaaaatt gctcatcaac caaccccgaa tggcatcagg
     3241 aacaatcatg gttcgattca ttttcgaaac cttgtattta ggaagtgaca gcaattcata
     3301 aaacttgcaa ttcttgccaa ggtacatgtc aagaccgatg gctaggttcg aatccgaaac
     3361 aactacatta taattaaaac cagaaatgag cgttgataca gca
//
LOCUS       DAOOQW010000248         3336 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_34181_length_3336_cov_4.340750, whole genome shotgun sequence.
ACCESSION   DAOOQW010000248 DAOOQW010000000
VERSION     DAOOQW010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3336
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34181_length_3336_cov_4.340750"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2693
                     /locus_tag="PK281_10810"
     CDS             <1..2693
                     /locus_tag="PK281_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007649661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY49877.1"
                     /translation="YANQYYQPVNPPFYANHAGDSNLVDPNRWQQIFLDQYIDQNGNV
                     YNISPPFLGAEWGNVHPFALTDSNKTVHSRDGHDYNVYLDAGPPPLLDSTDTSGITSD
                     YKAGYLMVSIWQAQLDTADGVMWDISPGAIGNIASYPETYTAGMEFYNFIDGGDMGTG
                     RALNPKTGLPYEPNIVHRGDYARILAEFWADGPNSETPPGHWFTILNYVGDQPQFEKR
                     WRGTGEIISDLDWDVRSYFALSGAMHDAAIAAWSNKGWYDSPRPISILRWMCGKGQCS
                     DSLLPNYHRAGTPLIPGYVELVMPGDPLAGVNNEHLYKIKLFSWRGHDFIPDPDVDMA
                     GAGWILAENWWPYQRSSFVTPPFAGYMSGHSTFSRTAAELMTLMTGDPFFPGGMCEFH
                     ATQNQYLVFEEGPSEDITIQWATYQDASDQCSLSRIYGGIHPPQDDLPGRKMGMFLGP
                     NALQHAEDIFHAGLPFVQNVTIDQDLISDQLAEGFVTISVTYNEDMDQLSQPTIEFLN
                     AVVSPSLTSSTVNWSSARTCQISFLVSDLDTNITQVGVKVSGGLDLESNEQKAYFQNN
                     LFDIDTRNPVLTSSSASNALVNMSLVGNGTFMVELTYDEPMNTTLTPSIVVSPTTVTS
                     ALAFNSGMSSWINDTTYQAAYDVIGNVGEPGNINFNYALGQDAAGNFQFAESSEFDVF
                     VDTRQPLVSILTANAYELTEQEVGLEALKFISIFDEEMDQTITPTITFPGNASAQSLL
                     LFNQDASYWINEQTYLASFDLDFQSIDVLDIPVLVSGAVDLRGNSQVASSNAAFLDLL
                     LDTTSVGMNDLATSFGVTSTYPNPVLSGAQLFIRIPGEVEKLQMKVYAMNGQLIRSEQ
                     INSIANGLYAVSTNNLSAGLYFVQLSDAKRTATFKVQVTD"
     gene            complement(2755..>3336)
                     /locus_tag="PK281_10815"
     CDS             complement(2755..>3336)
                     /locus_tag="PK281_10815"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY49878.1"
                     /translation="EYEYVLTDVAGCSFSQTIELDQPDSIRTYAEFNQTTCPNAWSGF
                     VVAEGGTVPYQYQWDFFYEGETIPFQSLDDKAFTCIQSPMNIHAKYQITDMHGCTKMG
                     TQILPGIVGMADLEANLETFYPNPVQQILHLNLKSAIQKLDLFDLSGRLIQTFALTES
                     KTQEIDLSNLVSGFYLLSYKQGDEPVVKRFVKE"
BASE COUNT          968 a          631 c          691 g         1046 t
ORIGIN      
        1 attatgctaa tcagtattat caaccggtaa atccaccttt ttatgcgaat cacgcaggtg
       61 atagtaattt agtagatcct aaccgttggc agcagatttt ccttgatcag tacattgacc
      121 aaaatgggaa tgtgtacaat atttcaccgc cattcttagg tgccgaatgg ggaaatgttc
      181 atccatttgc tcttactgat tcgaacaaaa cggtacactc tcgtgatggt catgactata
      241 acgtttacct cgatgcaggc cctcctcctc ttttggattc aactgataca tccggcataa
      301 cttccgatta taaagcaggt tatttaatgg tgagtatttg gcaagcgcaa ttagatacag
      361 ccgatggcgt gatgtgggat atttctccag gcgcaattgg gaatattgct tcgtatccag
      421 aaacctatac agctggaatg gaattttaca atttcattga tggtggtgac atgggaactg
      481 ggcgagctct caatcctaaa acaggacttc catacgaacc caatattgtt catcgaggtg
      541 attatgcacg tattcttgct gagttctggg ctgatggacc taattcagaa actcctccag
      601 gacattggtt taccattttg aattatgtag gtgaccaacc tcaatttgag aaacgctggc
      661 gaggtacagg tgagataata agtgatttag attgggatgt tcgaagctat tttgctttat
      721 ctggtgcaat gcacgatgca gctattgcgg cttggagtaa taaaggatgg tacgattcac
      781 ctcgtccgat ttcgatttta cgctggatgt gtggaaaagg acaatgttcc gattcgcttt
      841 taccaaatta tcatcgtgct ggcactccac ttatccccgg ttatgtggaa ttagttatgc
      901 cgggtgatcc actggcaggc gtgaataatg agcatctgta taaaattaaa ctcttctctt
      961 ggagaggtca cgattttatc cctgatccgg atgtagatat ggcaggtgct ggttggattt
     1021 tagctgaaaa ctggtggcct taccaacgtt ctagctttgt aactccacca tttgctggtt
     1081 atatgtcggg acactctacg ttttcgcgta cagcagccga actcatgacc ttaatgactg
     1141 gagatccatt cttcccagga ggcatgtgtg aatttcatgc aactcaaaac caatatttgg
     1201 tatttgaaga aggtccaagt gaggacatca ccattcaatg ggcaacttac caagatgctt
     1261 cggatcaatg cagtttatca cgtatttatg gaggaattca ccctccgcaa gatgatcttc
     1321 ctggaagaaa aatgggaatg ttcttaggtc caaatgcact tcaacatgct gaagatattt
     1381 tccatgctgg tttgcctttt gttcaaaatg taacaatcga tcaggattta atttcggatc
     1441 aattggcgga aggatttgtc actatttcgg tgacttacaa tgaagacatg gatcaacttt
     1501 ctcaaccaac tattgagttt ttgaatgcag ttgtttctcc ttcattaact tcttcaacag
     1561 taaactggag cagtgctaga acttgtcaaa tctccttctt ggttagcgat ttagatacaa
     1621 acatcactca agttggtgtt aaggtttctg gcggattaga tttagaatcc aacgaacaga
     1681 aggcttattt ccaaaacaat ttgtttgata ttgatactag aaacccagtc ctaacttcaa
     1741 gttcagcttc taatgcgctt gtaaatatga gcttagtggg aaatggaact tttatggttg
     1801 agttgactta tgacgaaccc atgaacacta cattaacacc ttccattgtt gttagtccga
     1861 ctacagttac ttctgcattg gcattcaatt caggaatgag ttcttggatt aatgatacaa
     1921 cttaccaagc agcttatgat gtaattggaa atgtgggtga accgggaaac atcaatttca
     1981 actatgcatt aggacaagat gctgcaggta atttccaatt tgcagaatct tccgaatttg
     2041 atgtctttgt tgatacgcgt caaccacttg tttccattct tacagctaat gcttatgaat
     2101 taaccgaaca ggaagtaggc ttggaagctt taaagttcat ttccattttt gatgaagaaa
     2161 tggatcaaac tatcacacct actataacat tcccaggaaa tgcaagtgct cagtcattgc
     2221 tattattcaa ccaagatgct tcgtattgga ttaatgagca aacgtatcta gcttcgttcg
     2281 acttagattt ccaaagtatt gatgtgttgg atattccagt ccttgtaagt ggagcggtag
     2341 atttaagagg aaattctcaa gttgcaagta gcaatgctgc attcctcgat ttacttttag
     2401 atacgaccag tgttggcatg aacgatttag ctacttcgtt tggagttacg agtacttatc
     2461 caaaccctgt attatcagga gcacagctct ttattagaat accgggcgaa gtagagaaac
     2521 tacagatgaa agtttatgcg atgaatggac aattgattcg tagtgaacag ataaattcaa
     2581 ttgccaatgg cttgtatgct gtttcaacta acaatctttc ggcaggactt tattttgttc
     2641 aattgagtga tgcgaaaaga acagctacat tcaaagtaca agtgactgat taaaaattca
     2701 tttagaacga aatcaaaaaa tgcagtcggg ttatcctgac tgcatttttt taagttattc
     2761 cttaacgaac ctcttcacaa ctggctcatc tccttgttta tagctcagca aatagaatcc
     2821 agaaacaaga ttcgataaat ctatttcttg ggttttagat tcagtaagag caaatgtttg
     2881 gatgagtctt ccgcttaaat cgaatagatc gagcttttga atagctgatt ttagattgag
     2941 atgaagaatt tgttgaaccg gatttggata gaacgtttcc aagtttgctt ctagatcagc
     3001 cattccaacg ataccgggta aaatttgcgt gcccatttta gtgcagccat gcatatcggt
     3061 tatttggtat ttagcatgaa tgttcatggg tgattgaata catgtaaatg ccttatcatc
     3121 aagagattga aatggaattg tttcaccctc ataaaagaaa tcccattgat actgataagg
     3181 aacggtacca ccttcagcaa ccacgaatcc tgaccaagca ttcggacaag tggtttgatt
     3241 aaattcagca tacgttctaa tcgaatcagg ttggtcgagt tctattgttt gagagaaaga
     3301 acatcctgca acatcggtta gaacatactc atattc
//
LOCUS       DAOOQW010000249         3320 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_34387_length_3320_cov_4.387749, whole genome shotgun sequence.
ACCESSION   DAOOQW010000249 DAOOQW010000000
VERSION     DAOOQW010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3320
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34387_length_3320_cov_4.387749"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1045
                     /locus_tag="PK281_10820"
     CDS             <1..1045
                     /locus_tag="PK281_10820"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOY49879.1"
                     /translation="EAVIFEKQGDLGKALQIQKEFNELSEGLYLASLTLQNTQQRILN
                     EIEQKNNELLEKNSTISKMSKMQIGLFIALILLLIGAAFLYQLNNQHKSDLIKIAKQS
                     EELSETIQYNEIQKKELEISNDTRTKLLGLISHDIRVPMYNSLALVEAMHDYKGDPKR
                     MNEVCTEVLSGLRITTQLIDNLLFWSRKNASQIHAEFEILNLKSCIETEMKLFSIIAA
                     GKKIAVINEIPDNLNVYADSQLIRMTIRNLVMNSIKFTPDGGTISIHASRINGHTEVR
                     VKDTGLGMNSDQIAHLFDEKHAFSTNGTHGEMGTGLGLMMVKEFVEKNKGIVHVESAP
                     GQGSTFSFTLKPV"
     gene            complement(1042..3066)
                     /locus_tag="PK281_10825"
     CDS             complement(1042..3066)
                     /locus_tag="PK281_10825"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOY49880.1"
                     /translation="MSTPNLKFTNALIDSSSPYLLQHAHNPVNWYPWGEEALEKAKRE
                     NKPLLVSIGYSACHWCHVMEHESFEDEETAALMNAHFVCIKVDREERPDIDQLYMAAV
                     QLMTQKGGWPLNCFALPDGRPIYGGTYFPKENWQHVLKQIDSLYKNDWDKVQEYAGNL
                     TKGIEQMELFKVKDGVESISKNDLQESVGKWLGMIDQEEGGPNRAPKFPLPSNYLFLL
                     RYSVLTNQKHVLEHVHLTLKKMAFGGIYDQIGGGFSRYSTDVWWKVPHFEKMLYDNGQ
                     LLSLYAEAYQQYADEDYRAICMQTAEFVKRELTSKEGYFFSALDADSEGVEGKFYTWK
                     LDELKAVLSEEEYLVARVYYNLNDFGFWEHEQYILLRRDENRIVAEVLELDEIELENH
                     IRKINSKLMAARDKRVRPGLDDKLLCSWNAMMIRGLADASRIFDEEQLLVDAEKAARF
                     ILQELKDEEGRLWHTWKNGKRSVPGFLEDYAFMIDALIGLYEASYSDHWLLEARDLMY
                     TVLGDFERSASGLFYFTSSQHGVWVAKQLETSDNVQPASNSMMARNLHTLGIYLGKPE
                     WMQQTNSMLRTVRDELINYGPGYSNWGLLVLEQEYDFRELVISGIGAIKAAKQIRKKY
                     RPNLLLAASETTTLLPLFEGRNPGEKPKFYLCKGMMCESPVNSIEELNLN"
     gene            complement(3147..3296)
                     /locus_tag="PK281_10830"
     CDS             complement(3147..3296)
                     /locus_tag="PK281_10830"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOY49881.1"
                     /translation="MKESHLELVANIPGISKEKFDEIVADAEKNCPISKLLNTTISVN
                     AQLIS"
BASE COUNT         1055 a          769 c          519 g          977 t
ORIGIN      
        1 cgaggcagtc attttcgaaa agcaaggtga tctgggaaaa gcactacaaa ttcaaaaaga
       61 atttaatgaa ttatcagaag gtttatatct cgcttcgcta accttacaga atactcaaca
      121 gcgcatactc aacgaaatag aacagaagaa caacgaacta ctagagaaaa atagtacgat
      181 atcaaagatg tcgaaaatgc aaatcggttt attcatagca ctcattttat tgcttatcgg
      241 tgctgccttt ttgtatcaac taaataatca acacaaatcc gatttaatca aaatagctaa
      301 acaatctgaa gagctttctg aaacaatcca gtataacgaa atacagaaga aagaactcga
      361 aatctccaat gacacacgta ctaaattatt aggtcttatt agtcacgata ttcgtgttcc
      421 tatgtacaac tctttagctt tggtagaggc catgcacgat tacaaaggcg atcccaaacg
      481 tatgaatgag gtttgtactg aagtgctttc tggattacga attaccactc aactcatcga
      541 taatctatta ttctggtctc gaaaaaacgc ttcccaaatt catgcagaat tcgaaattct
      601 caatcttaaa tcctgcatcg aaaccgaaat gaagcttttt tctatcatag ctgcaggaaa
      661 gaaaattgct gtgataaacg aaattcccga taacctaaac gtatacgccg atagtcaact
      721 cattcgaatg accatacgaa atctagtcat gaattctatc aagtttactc ccgatggcgg
      781 aactattagc attcatgcta gtcgaataaa cggacatacc gaagtacgcg taaaagacac
      841 aggcttaggc atgaatagcg accaaattgc tcatctattc gatgaaaaac atgccttcag
      901 caccaatgga acgcacggcg aaatgggaac tggattaggt ttaatgatgg tgaaagaatt
      961 tgtagagaaa aacaaaggta ttgtgcatgt agaaagtgct cccggacaag gaagtacatt
     1021 cagctttacc cttaaacctg tttaattcaa attcaattct tctatcgagt ttacgggact
     1081 ttcacacatc atccctttac acaaataaaa cttaggcttt tctcccggat ttcttccttc
     1141 aaacaaaggg agtagggtag tagtttcgct tgctgcaagt agtaaattag gtcgatactt
     1201 ttttcgaatc tgtttggctg cttttattgc accaattcca ctgatgacta attctctaaa
     1261 gtcatattct tgctctaata ccaataagcc ccaattcgaa taacccgggc catagttaat
     1321 cagttcatct cgcacagttc gtaacatcga attcgtttgt tgcatccatt cgggtttgcc
     1381 caagtaaatt ccaagcgtgt gaagatttct cgccatcatt gaattggacg ctggctgaac
     1441 attatccgaa gtctctaact gcttagccac ccaaacacca tgttgcgacg aagtgaaata
     1501 aaacaaacca cttgcacttc gttcaaaatc gcctaaaacg gtatacatca aatcacgcgc
     1561 ttccaataac caatgatccg aatagcttgc ttcatacaag ccaatcaagg catctatcat
     1621 aaaggcataa tcttctaaaa atccaggaac gctgcgtttg ccatttttcc aagtatgcca
     1681 caatctgcct tcttcatctt tcagttcttg taagataaat cgagctgctt tttcagcatc
     1741 taccagtaat tgttcttcat caaaaatgcg tgatgcatct gccaaaccgc gaatcatcat
     1801 cgcattccaa ctacataata atttgtcatc caatcctgga cgaacgcgct tatctcgtgc
     1861 tgccatcaat ttcgaattaa tcttccgaat atgattttcc aactcgattt catctagttc
     1921 cagaacttca gctacaattc gattttcatc gcgccgcaac aagatgtatt gctcatgttc
     1981 ccaaaatccg aaatcattca aattataata aacacgtgca acaaggtatt cttcttcact
     2041 cagaactgcc ttcaactcat ccaatttcca agtgtaaaat tttccttcaa ctccttcgct
     2101 atcagcatct aatgccgaga aaaaatagcc ttcttttgat gtcagttctc gcttcacaaa
     2161 ctcagctgtt tgcatacaaa tagcccgata atcttcatcg gcatactgct gatatgcttc
     2221 cgcatacaaa ctcagtaatt gcccattatc atacaacatt ttctcaaaat gtggaacctt
     2281 ccaccacaca tccgtcgaat acctcgaaaa accaccgcca atctgatcgt aaattccacc
     2341 aaatgccatc tttttcaagg tcaaatgcac atgctctaac acatgctttt gattcgtcaa
     2401 caccgaataa cgtagcaaaa acaaataatt cgaaggcaaa gggaatttag gtgcacgatt
     2461 cggacctcct tcctcttgat caatcattcc cagccatttt cccacacttt cctgcaaatc
     2521 gtttttacta atcgattcta ctccatcttt cactttaaac aattccatct gctcaattcc
     2581 tttggtcaaa ttgccagcat attcttgcac tttgtcccaa tcatttttgt aaagcgaatc
     2641 aatctgtttc aacacatgct gccagttttc cttaggaaaa taagtacctc cgtaaatcgg
     2701 tcttccatca ggtaaagcaa aacagtttaa tggccatcca cctttttgtg tcattaattg
     2761 aacagctgcc atatataatt ggtcaatatc aggccgctcc tctcgatcaa ctttaataca
     2821 cacgaaatgt gcattcatca aagccgcagt ttcctcatcc tcaaacgatt catgttccat
     2881 cacatggcac caatgacaag ccgaataacc aatacttact aataagggtt tgttttcacg
     2941 tttggctttt tcgagcgctt cttctcccca aggataccaa ttaaccggat tgtgagcgtg
     3001 ttgcaacaaa tacggactgc ttgaatcaat taatgcattc gtgaatttga ggttgggtgt
     3061 cgacatgcca aatatttttt acaaacctac aatacaaaag caccgaaatt cacgaagaac
     3121 ttcggtgctt aattaagctg ttaaattcaa gaaattaact gtgcatttac agaaatagta
     3181 gtattgagca acttcgaaat cggacagttt ttttcagcat cagctacaat ttcatcaaat
     3241 ttctccttcg aaattcctgg aatattggcc actaattcta aatgcgattc ttttatcgaa
     3301 ccatcttcaa acgtaatggt
//
LOCUS       DAOOQW010000250         3301 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_34651_length_3301_cov_3.877696, whole genome shotgun sequence.
ACCESSION   DAOOQW010000250 DAOOQW010000000
VERSION     DAOOQW010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3301
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34651_length_3301_cov_3.877696"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1159
                     /locus_tag="PK281_10835"
     CDS             <1..1159
                     /locus_tag="PK281_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HOY49882.1"
                     /translation="PVLFAAALLLIFDRSFGTSFYLSDIYIAGEALSNTGGSPVLFQH
                     LFWFLGHPEVYIVLLPALGLSSEIIATNARKPIFGYKAMIGSMLFIAILSFIVWAHHM
                     FVTGMNPFLGSVFMLLTLIIAVPSAVKIFNYVTTLWKGNIRFTPGMLFSIGLVSFFLT
                     GGITGIFLGNSAVDIQLHDTYFVVAHFHLVMGSASFFGMMAGVYHWFPKMFGRMMNGK
                     LGTIHFWLTFVGVYLVFFPMHYIGIAGFPRRYYSWTNFDTFSGFTDLNMLVSVAAILT
                     LSAQFIFLFNFFYSIFRGRAASENPWESTTLEWTTPRHPGHGNWVGEIPSVYRWPYDY
                     SKPGSKEDFIPQTIPYSETPESNLPHENDLIKLEGDGDDAIVVKAALSTKK"
     gene            1241..2251
                     /locus_tag="PK281_10840"
     CDS             1241..2251
                     /locus_tag="PK281_10840"
                     /inference="COORDINATES: protein motif:HMM:NF014665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HOY49883.1"
                     /translation="MRSFRRLCLSTLIAVYILILVGGVVRSTGSGMGCPDWPKCFGSW
                     VPPTDVSELPENYKEIYAAYRDKKNQRFAKYLTTFGFDETASKILTNPQVLHENDFNA
                     TKTWIEYINRVVGVIIGLLIFALFIFSIRLWNKERKLTMASFATLILVGFQGWFGSFV
                     VSSNLTAWTITVHMFLALVIVGILIYLFKESNYGQNITIASTYGWVLACMAALLIQIF
                     FGTQVREGIDRVALTFERESWIDNLGRDFVIHRTFSWIVLILHVILILKLRKTEAAKI
                     FALALIMLLLGTVLAGVAMSYFAVPAFLQPIHLLLATVTFGILFMLILRVNIRSESVL
                     NN"
     gene            2253..3149
                     /gene="cyoE"
                     /locus_tag="PK281_10845"
     CDS             2253..3149
                     /gene="cyoE"
                     /locus_tag="PK281_10845"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HOY49884.1"
                     /translation="MITGGIDQLNGISLARAKGKAYWELLKPRLSFLVAFSCAFGFVL
                     ASSRIDILTLSMLFIGGFLLSGSSVIVNQIIEIDLDKLMVRTMKRPLPTARLSIRESV
                     IFAIVCFIVGVGILILFTNWLTTSLSILSMVLYSFVYTPLKRVGPIAVFVGAIPGALP
                     PLLGWTAATGLISHETLIIFGIQFIWQFPHFWAIAWVSDDDYKKAGFKLLPSGGKKDL
                     NTAVQIMIYTMFLIPMGMLPATFGITGIKSAIVATICGVLFFALTFSLMKSGDRKSAL
                     KIMFASFLYLPIVQIAYMLDKV"
BASE COUNT          810 a          622 c          734 g         1135 t
ORIGIN      
        1 ccctgtattg tttgctgccg ctttgttatt gattttcgat agaagctttg gaacaagttt
       61 ttacttgtct gatatctaca tagcaggaga ggcgctttcc aataccggag gtagccctgt
      121 tttgttccag catttgttct ggttcttagg tcaccctgaa gtgtacatcg tattgcttcc
      181 tgctcttggt ctatcttctg aaatcatagc gactaacgca cgtaaaccaa ttttcggtta
      241 caaggccatg atcggatcta tgcttttcat tgctatctta tcgttcattg tttgggcgca
      301 tcacatgttt gtaaccggta tgaatccgtt cctcggttcg gtcttcatgt tgcttacgct
      361 gattattgca gtaccctctg cggtgaagat tttcaattat gtaaccacat tatggaaagg
      421 taatatccgc tttacacccg gtatgctttt ctcaatcgga ttggtttcgt tcttcttaac
      481 gggaggtatt acaggtatct tcttaggtaa ttctgcggtt gatattcaac ttcacgatac
      541 ctatttcgtg gtggctcact ttcacttggt aatgggtagt gcatcgttct ttggtatgat
      601 ggccggggtt tatcattggt tcccaaagat gtttggacgc atgatgaacg gcaaattggg
      661 tacgatacat ttctggttaa cttttgttgg tgtatatctc gtgttcttcc caatgcatta
      721 cataggtatt gcaggtttcc caagacgtta ttattcatgg acgaattttg atacgtttag
      781 tggtttcaca gatttgaata tgctggtaag tgttgccgct attcttaccc tgagtgcaca
      841 gtttattttc ttattcaatt tcttctacag cattttccgc ggtagagcag cttcagaaaa
      901 tccatgggag tctacaacct tagaatggac aacaccgcgt catccgggtc atggaaactg
      961 ggtaggtgaa ataccttctg tttacagatg gccgtatgat tacagcaagc ctggctcgaa
     1021 agaagatttt attccgcaga ctattccata ttctgaaact cccgagtcta atttacctca
     1081 cgaaaacgat cttattaaat tggaaggtga tggagatgat gccattgtag tgaaggcggc
     1141 cctaagcact aaaaaataat ttagtatcaa ggttccagat tcaaggttca aagttataag
     1201 cattgaacat ggaatctgga accttgaact tttataaact atgaggtctt ttcgcagact
     1261 atgtctttct acactcatcg cagtttacat tctcattctt gtcggtggtg tggtacgcag
     1321 cacaggctca ggcatgggtt gtccggattg gccaaaatgt tttggtagct gggttccacc
     1381 aactgatgtg tctgagcttc ctgaaaatta taaagaaatc tacgctgctt atcgcgataa
     1441 aaagaatcaa cgttttgcaa aataccttac gacatttggt ttcgatgaaa cggcttcaaa
     1501 aatccttact aatcctcaag ttcttcacga gaatgatttc aatgcaacca aaacatggat
     1561 tgaatacatc aatcgtgtag tgggtgtgat cattgggtta ttgatttttg ccctcttcat
     1621 tttctctatt cgcctttgga ataaagaacg caagcttact atggcctctt ttgccacctt
     1681 aattttagtt ggatttcaag gatggtttgg ttcgtttgtc gtatccagta atttaaccgc
     1741 gtggacgatc acggttcata tgttcctggc tttagtcatt gtaggcattc taatttactt
     1801 gttcaaggaa agtaactacg gtcaaaacat tacgattgcc tctacctatg gatgggtact
     1861 ggcgtgcatg gctgctttgt taattcagat attttttggg acacaggtgc gcgagggcat
     1921 tgaccgagtg gccttgactt ttgagcgtga atcatggatt gacaaccttg gaagagattt
     1981 cgttattcat cgcacattct cctggattgt gctcattctg catgtgatat taatattgaa
     2041 attgcggaaa accgaggctg caaaaatttt cgcactagcg ttaatcatgt tacttttggg
     2101 gactgtttta gcaggtgttg caatgtccta ttttgctgtt ccagcgttcc ttcagccgat
     2161 tcatttgctt ttggcgaccg ttacctttgg aatactcttc atgttgatat tgcgagtaaa
     2221 catacggtct gaatcagtgt taaataattg agatgattac aggaggtata gatcagctaa
     2281 atggaatttc acttgcacgc gcaaaaggta aagcctattg ggagttgttg aaacctcgtt
     2341 tgtcttttct ggttgcattc tcctgtgcgt ttggttttgt attagcatcc tcccggatcg
     2401 atatccttac cttatcgatg cttttcatcg gaggattttt gctttccgga tcttccgtta
     2461 ttgtaaatca aattatcgaa attgatctgg ataaactcat ggtgcgaacc atgaaaagac
     2521 cgttaccaac agcacggttg tccatccgcg aaagcgtaat atttgccatt gtttgtttta
     2581 tagttggcgt aggaattctt attctcttta ccaattggct cacaacatcc ttgtcgattt
     2641 tgtcgatggt gctttacagt tttgtgtata ctccgctaaa acgagttgga ccgattgctg
     2701 tttttgttgg cgcaattcca ggggcattac ctccgctttt aggatggact gctgcgacag
     2761 gattaatatc gcatgaaacg cttattattt tcgggatcca attcatctgg cagtttccgc
     2821 atttctgggc cattgcctgg gtgtcggatg atgattataa aaaagcagga tttaaattac
     2881 ttccttccgg aggaaagaaa gatttgaaca ctgctgttca gattatgatt tacaccatgt
     2941 tcttaattcc gatgggaatg cttccggcta cctttggaat tacaggaatt aaatcggcta
     3001 ttgttgctac gatttgtggt gtgttgtttt ttgcacttac cttttcattg atgaaatcag
     3061 gtgatcggaa gtcggctttg aaaattatgt ttgcatcgtt tttatacttg ccgattgtgc
     3121 agatcgcata tatgttggat aaggtttgat gatttgttga tgagttgatt tgagactgag
     3181 aggaatttgg tgaggttgat ttggattaaa gaaaaattat ggcggcagag attaaaatag
     3241 tggaggaggc ggataagccc attggcatga acccgaagaa attcgcgctg tggttattca
     3301 t
//
LOCUS       DAOOQW010000251         3268 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_35123_length_3268_cov_4.947401, whole genome shotgun sequence.
ACCESSION   DAOOQW010000251 DAOOQW010000000
VERSION     DAOOQW010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3268
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35123_length_3268_cov_4.947401"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="PK281_10850"
     CDS             complement(<1..264)
                     /locus_tag="PK281_10850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49885.1"
                     /translation="MKKLLLVVAVVFATSLLKAQSTFTQINSLFQNSCAIGCHNGVDN
                     SGNLNLSGTDSEVYNRLVNVSPTNPAAAAKGYKLIRPGYPDRSF"
     gene            complement(274..984)
                     /locus_tag="PK281_10855"
     CDS             complement(274..984)
                     /locus_tag="PK281_10855"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOY49886.1"
                     /translation="MAYTYYECEVIQILDETPDVKRFVLRYPSEVKLDFHAGQFVMLD
                     LPIDSKYTNRSYSIASPPSSDNTLELIIVMNPQGLGTPHLFRDVTVGSKIKTTLPLGK
                     FRLDENLNRRVCFICTGTGIAPFRSMILDIFQQEKPHQEITLIAGVRSQRDLLYHEEF
                     LKLQEEKASFNYIPVLSREVWAGRSGYVHDVYKELFPMLGDHLFYLCGWANMLKEARE
                     HLQEFVLDKKQIRFESYD"
     gene            complement(1106..2977)
                     /locus_tag="PK281_10860"
     CDS             complement(1106..2977)
                     /locus_tag="PK281_10860"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HOY49887.1"
                     /translation="MATLVVGIPANAEAQQAPVIPLKDFFRNPEKVAFRISPDGKHLA
                     FMQPWENRLNIFVQEIGKPGEVQVTFAKERDIQGYFWKNNNRLVFLQDKGGNENFHLF
                     AVDSDGKNQKELTEGADVRAEIIDDLYDNDNEMIIGLNKRNPQFYDAYRININTGELT
                     MVGENPGNITGWQTDNKGVIRIATTTDGINTGVLYRNNDTEKFTELISTNFKESFSPL
                     YFDFDDKLLFVSSNIGRDRSTIIKYDPVAKKEISSLYEHPEVDVSTLLRSRKRKVITG
                     VVYDTDKSHYQFFDKQREELQKKLDTRFPGYEVSLAGQNRNEDKLLITVSSDRSYGSY
                     YFYDLTTGAFTKLADISPWLKEEDMAEMKPIKYIARDGKVINGYLTLPKGIPAKNLPV
                     VINPHGGPWARDEWGFNPEVQFLANRGYAVLQMNFRGSVGYGRAFWEASFKQWGLTMQ
                     NDITDGVNWLIEQGIADPKHVAIYGASYGGYATLAGITLTPDLYACAVDYVGVSNLFT
                     FMSTIPPYWKPYLDMMYEMVGDPEKDKELMTAASPVFHVDKIKCPLFIAQGANDPRVA
                     KAESDQMVEALKKRGIDVPYMVKDNEGHGFGNEENRMDFYREMERFFGKYLNGRVEK"
BASE COUNT          935 a          679 c          641 g         1013 t
ORIGIN      
        1 aaaactccga tcgggataac ctgggcgaat gagtttgtac ccttttgcag cagctgcagg
       61 gtttgtggga cttacattca ccaatcgatt gtacacttcc gagtctgttc cactcaaatt
      121 caaatttcct gaattgtcaa ctccattatg gcagccaata gcacacgaat tttggaataa
      181 agagttgatt tgagtgaatg tactttgagc ctttaatagg cttgttgcaa atacaactgc
      241 aacaacaagt aaaagttttt tcatgggttg ggtttagtca tatgattcga accggatttg
      301 ttttttgtcc agtacaaatt cttgtaaatg ttctcgggct tctttaagca tgtttgccca
      361 tccgcaaaga taaaatagat ggtcacctag cataggaaat aattctttgt aaacatcatg
      421 cacatatccc gaacggccag cccaaacttc gcgcgataaa acgggaatgt aattgaagct
      481 agctttttct tcctgtagtt tgaggaattc ctcgtggtag agcaaatctc gttgacttct
      541 cactccagca atgagtgtta tttcttgatg aggtttttct tgttggaata tatctagaat
      601 catcgaccta aaaggagcaa tgcctgttcc cgtgcagata aaacataccc gacggttgag
      661 gttttcatct aagcgaaatt ttccaagtgg aagtgttgtt ttgattttag aaccaactgt
      721 tacatcccga aataaatgtg gtgttcccaa accttgtgga ttcatcacaa tgatgagttc
      781 taaggtattg tcgctcgatg ggggagaggc aattgaataa cttcggttgg tgtatttgct
      841 gtcgattggt agatccaaca tcacaaactg accagcatga aaatctagct ttacttcact
      901 gggatagcgt aaaacaaagc gtttcacatc tggagtttcg tccagaatct gaattacttc
      961 acattcataa taggtatacg ccatttcaaa ccaagatact gaaaatattc atccggctca
     1021 ttttcaaaag attgaaatgg agcagactcc ataaaaaatg cccgggactt tcacatcacc
     1081 gggcaccttc aatcaattga atggtttatt tctcaactct accattcaag tatttaccaa
     1141 agaaacgctc catctcacga taaaaatcca ttcggttttc ttcgttacca aagccgtgtc
     1201 cttcgttgtc tttcaccata tatggcacat caattccgcg ttttttcaag gcttcaacca
     1261 tttggtccga ttcagctttc gctacacgtg gatcgttagc accttgagca ataaagagcg
     1321 gacatttgat tttatctaca tgaaaaacgg gtgaagcagc cgtcatcaat tctttatctt
     1381 tttcaggatc acctaccatt tcgtacatca tatcgagata tggtttccaa taaggaggaa
     1441 ttgtactcat gaatgtgaaa aggtttgaaa cacctacgta atcaactgcg cacgcatata
     1501 aatcgggtgt taatgtgata ccagcaagcg ttgcataacc accataagat gcaccataaa
     1561 tagctacatg ttttggatca gcaatgcctt gttcaatgag ccaattcaca ccatcggtaa
     1621 tgtcgttttg catggttaaa ccccattgct tgaaactagc ttcccagaaa gcacgaccat
     1681 aaccaaccga acctctaaag ttcatctgca aaactgcata tccacgattc gctaaaaact
     1741 gaacttccgg attaaagccc cactcatcgc gagcccaagg tccaccgtgc ggattaatta
     1801 ctacgggtag gtttttagct ggaatgcctt tcggtaaagt caaatatcca ttaatcactt
     1861 tgccatcgcg agcaatgtac ttgataggct tcatttcggc catatcttct tccttcaacc
     1921 aaggactgat atcagccaat tttgtgaatg ctccagttgt taaatcgtag aagtaatagc
     1981 ttccataaga acgatcgctc gaaacggtaa tcagtaattt atcctcgttg cgattttgtc
     2041 cagctaaact aacttcatag ccaggaaaac gggtatctag tttcttttga agttcctcac
     2101 gttgtttatc gaaaaactgg tagtgacttt tatctgtatc atacacaacg ccagtaatga
     2161 ctttgcgttt gcggctacga agcaaagtgc ttacatctac ttcgggatgc tcgtataaag
     2221 aagaaatctc ttttttcgct actggatcgt atttgataat cgttgatcta tcgcgtccaa
     2281 tgttggaaga aacaaaaagc aatttatcgt cgaaatcgaa atacagcgga ctaaacgatt
     2341 ctttgaaatt ggtcgagata agttcggtga atttttctgt gtcgttattt cggtaaagca
     2401 cgccggtgtt gattccatcg gtggtagtag caatacgaat aacaccttta ttgtcggttt
     2461 gccagccagt aatattccct gggttttcgc caaccattgt taattcacct gtattgatgt
     2521 tgatacggta agcatcgtaa aattgaggat tacgtttgtt cagaccgata atcatctcat
     2581 tgtcgttatc gtacaaatcg tcgatgattt cggcacgtac atctgcacct tccgtgagtt
     2641 ccttttggtt tttaccatcc gaatcaacgg caaacaagtg aaaattttcg tttccacctt
     2701 tatcttgtaa aaaaactaag cggttattat ttttccaaaa atatccttgg atatcgcgct
     2761 ctttagcgaa cgtaacttgt acttcacctg gtttaccaat ttcttgaacg aagatgttca
     2821 agcggttttc ccaaggttgc ataaaggcaa gatgttttcc atcgggagaa attcgaaatg
     2881 cgactttctc aggattacgg aaaaaatcct taagtggtat tactggagcc tgctgagcct
     2941 cggcattggc agggattccg actacgagtg tagccataag tatcgatgca ataaagtttc
     3001 tagttgtaaa cataagggac aagagtgtat gaaaggggca aattaaaggt ttcttgttta
     3061 cttgaaaatc atttgaaaaa atgaaatgtc gtttgaaatt tgaaatttag tctgttctca
     3121 gcattagtag tttgaaatga ataattgtta caaaggagca ttatttttgg ggcttccccc
     3181 gtatttgcta cgtttcactt cacaaattcg gggtcgcgtc ttcgccttta ctcctccttc
     3241 gtgcggggta tcggctcaac cgtaaacc
//
LOCUS       DAOOQW010000252         3260 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_35238_length_3260_cov_5.958814, whole genome shotgun sequence.
ACCESSION   DAOOQW010000252 DAOOQW010000000
VERSION     DAOOQW010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3260
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35238_length_3260_cov_5.958814"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(198..812)
                     /locus_tag="PK281_10865"
     CDS             complement(198..812)
                     /locus_tag="PK281_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013304650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HOY49888.1"
                     /translation="MHVAVAGNIGSGKTTLTSLLSKHYNWVPHFEDADDNPYLNDFYE
                     DMQRWSFNLQIYFLNSRFNQVNDIRKSGKHVIQDRTIYEDAYIFAPNLHAMGLMTTRD
                     FENYFTLFNLMSSFIAPPDVLIYLRASVPTLVNQIQKRGRDYENSIRLDYLKRLNERY
                     EAWISNYTVGKLMIVDVDENKFSENPEHLGEIINRIDAEINGLF"
     gene            complement(928..2157)
                     /locus_tag="PK281_10870"
     CDS             complement(928..2157)
                     /locus_tag="PK281_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOY49889.1"
                     /translation="MNKPQTWTLSNGIRVIYTLVNSPVAHCGLFINAGSRDELNHEHG
                     LAHFIEHSVFKGTPKRKAYHILSRMEDVGGEINAYTSKEETVLYGTFLSAHYQRAIDL
                     LNDIAFHSSFPEKEINKEKTVIIDEINSYKDSPSEEIYDRFEEQIYPNHSLGHDILGT
                     PAHLKKFSPADIKSFISRTWNTDQIVFSFVGNIPVSRVKAQIDRTIGLQPANLRNFNR
                     QPVPVYAPTHQIEKRKNHQAHLVMGNLAYSVKDPKRTGLILLNNLLGGPGMSSRLNLA
                     IREKYGWTYNIDSGYAIFSDTGLWSVYLGTEAAWVDRCRELVLIEMKKLREKELGVLQ
                     LQKAKNQLIGQMAIAQESNSSLMLGFAKTFLIFNKIDTFEEVVQKIESLSSKDLRDIA
                     NEVFNESQLSSLLYQGR"
     gene            complement(2150..2401)
                     /locus_tag="PK281_10875"
     CDS             complement(2150..2401)
                     /locus_tag="PK281_10875"
                     /inference="COORDINATES: protein motif:HMM:TIGR03833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YwbE family protein"
                     /protein_id="HOY49890.1"
                     /translation="MQAQPATAASSNGCYRHQLHLNQLVDIVTKRDQKTGLLTRGRIR
                     WILTSKDYHSRGIKVMLVDRTVGRVQRIVTDPELHSSNE"
     gene            complement(2367..2777)
                     /locus_tag="PK281_10880"
     CDS             complement(2367..2777)
                     /locus_tag="PK281_10880"
                     /inference="COORDINATES: protein motif:HMM:NF025442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4258 domain-containing protein"
                     /protein_id="HOY49891.1"
                     /translation="MSFGRRLRLFIFGILLGSLVVWGFLFRGRTFPAWTPEGRVLESL
                     QEHPVKISTEARCLLDCNKISDQDILKLIADGDVLFSQSDIHGKEIPEYVVEGKGASG
                     KTFKMKFRTEYLSTLLITIIPTPDASSTCDCSVK"
     gene            complement(2817..>3260)
                     /locus_tag="PK281_10885"
     CDS             complement(2817..>3260)
                     /locus_tag="PK281_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HOY49892.1"
                     /translation="ATADVVIGALRAKEGRTPIVVTDEMVSNMKFGSVIVDISIDQGG
                     CFETSQVTNHANPVFKKYGVTHYCVPNVASRVSQTASLALSNIFTPILLDMGEKGGIE
                     NYLRMNAGVRNGVYIYNGIITHKYIGETYNIPYKDLDLLIAATQF"
BASE COUNT          967 a          649 c          657 g          987 t
ORIGIN      
        1 taaaacatta aaaagccctg taacatcatc gttacagggc tttttttata tcagtttgct
       61 tcttggactt tgtatgagcg gttgaggcgg tagcccgcag tgtgcgccgt agtgaaagcg
      121 aaggcgaaca agaggactta tagcccacat agtggcaagc aaaagcacga cccgaagggg
      181 cataccccga aaaagaatca gaatagaccg ttaatttcgg cgtcgatgcg gttgatgatt
      241 tctcctaaat gctccggatt ttcgctgaat ttgttttcat ctacatccac aatcatgagt
      301 tttccaacgg tgtagttcga aatccacgct tcataacgct catttaaacg ttttaaataa
      361 tcgaggcgga tcgagttttc ataatccctt ccacgttttt ggatttgatt caccaaggtt
      421 ggaacacttg cacgaagata aatcaacaca tctggcggag cgataaagct cgacatgagg
      481 ttgaataaag tgaagtagtt ttcgaaatcg cgtgtggtca ttaaacccat cgcgtgcagg
      541 ttgggagcga agatatacgc atcttcataa atcgtacgat cctgaatgac atgcttaccg
      601 ctcttacgaa tgtcgttaac ttgattgaat cgagaattca agaagtaaat ctgcaagttg
      661 aaagaccaac gttgcatatc ttcgtaaaaa tcgttcagat agggattgtc gtctgcgtcc
      721 tcgaaatggg gaacccagtt gtagtgtttc gacaacaatg aagtgagtgt ggtctttccg
      781 gaaccaatat ttccagcaac agctacatgc atagtattta tttgagccgc ctaaaatacg
      841 ccagttcctt cgttttcaaa acccgaatgt taaaaaacac ccaatttgag ttttatttct
      901 ttcgatttaa atataaatca ctgtaaatca acgaccttga tagagtaaag aagagagttg
      961 tgactcatta aaaacttcgt tagctatatc tcgtaagtct ttggaactga gggattcaat
     1021 tttctgaact acttcctcaa atgtatctat cttattgaat atcaggaatg tcttggcaaa
     1081 tcctaacatt aaagaggaat tgctttcttg ggcaattgcc atttgaccga ttaattgatt
     1141 tttggctttt tgcaattgta aaacgcctaa ttccttttct cgaagcttct tcatttcgat
     1201 taataccaat tctcgacacc gatctaccca agcagcttcg gttcccagat atacactcca
     1261 caatccagta tcactgaaaa ttgcatagcc cgaatcgata ttatacgtcc aaccatattt
     1321 ttctcggatg gctaagttga gtcttgaact cattcctggt ccgccgagta gattgtttag
     1381 taatatcaat ccagtacgtt taggatcttt aaccgaatac gccaaattgc ccattactaa
     1441 atgcgcttga tgatttttgc gtttttcgat ttgatgggta ggagcataaa caggaaccgg
     1501 ttgtcgattg aaatttcgaa gattggcagg ttgcaatcca atggttcgat cgatttgtgc
     1561 tttaactcga gaaacgggga tgtttcctac aaatgaaaat acaatttgat cggtgttcca
     1621 agtgcggcta ataaatgatt taatatcagc aggactgaat tttttcaaat gagcaggagt
     1681 tcctaaaata tcgtgtccga gtgaatggtt cggatagatt tgttcttcaa agcgatcgta
     1741 aatttcttcg gaagggctgt ctttgtagga atttatctca tcgatgatga ccgttttttc
     1801 tttgttgatt tccttttcgg gaaaactcga atgaaaggca atgtcgttga gtaaatcaat
     1861 tgcacgctga taatgagccg atagaaaagt gccgtataaa acagtttctt ctttgctggt
     1921 ataagcatta atttctccac ccacatcttc cattcgacta agaatgtgat aagctttacg
     1981 tttgggagta cctttaaaaa cagaatgttc gataaaatgt gctaaaccgt gttcgtgatt
     2041 caattcatcg cgactaccag cattgataaa caagccacag tgagcaaccg gactatttac
     2101 taaagtgtaa ataacacgga ttccgtttga taatgtccag gtttggggtt tattcattcg
     2161 acgaatgtag ttcaggatcg gtcacaatgc gctgaacacg accaactgtt ctatccacta
     2221 acatcacttt tatgcctctc gaatggtaat ctttactggt gagaatccaa cgaatgcgac
     2281 ctcgggtaag caatcccgtt ttttgatccc gtttggttac gatgtcgacc aattgattga
     2341 ggtgcaattg atgacggtaa cagccattac ttgacgctgc agtcgcaggt tgagcttgca
     2401 tctggagttg gaataatggt aatgagtaat gtgcttaggt attcggtacg aaacttcatt
     2461 ttaaatgtct ttccagatgc acctttacct tcaacgacat attcaggaat ttcttttcca
     2521 tgtatatcac tttgactaaa aagcacatcg ccatcagcaa ttagtttgag aatatcttgg
     2581 tcactgattt tattgcaatc gagcagacaa cgagcttcag tagatatttt gactggatgt
     2641 tcttggagtg attcaagcac tcgcccctcg ggagtccaag ctgggaaagt tctaccacga
     2701 aaaaggaatc cccaaacaac taagcttcca agaagaattc cgaaaataaa taagcgaaga
     2761 cgacgaccaa aactcatgcg gcaaaagtag tagaaaatgt tcgggtaagc ctcgactcaa
     2821 aattgagttg cagcaataag taaatcaagg tctttatagg gaatgttata cgtttcgcca
     2881 atgtatttat gggtgataat accattatat atgtatacac catttcgaac tccagcattc
     2941 attcgcaaat aattttcgat accacctttt tctcccatat ctaataaaat aggagtgaag
     3001 atattactca aagcgagcga agctgtttga gatacgcgtg atgccacatt cggtacacaa
     3061 tagtgtgtaa caccgtattt tttgaaaacc ggattagcat gattggtcac ttgagaagtt
     3121 tcgaaacatc caccttggtc aattgaaata tccacgatga ctgaaccaaa tttcatgttg
     3181 ctcaccattt catcggtaac gacaatgggt gttctacctt cttttgcacg taatgctcca
     3241 ataacaacat cggcagtagc
//
LOCUS       DAOOQW010000253         3251 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_35369_length_3251_cov_6.737484, whole genome shotgun sequence.
ACCESSION   DAOOQW010000253 DAOOQW010000000
VERSION     DAOOQW010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3251
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35369_length_3251_cov_6.737484"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..917)
                     /locus_tag="PK281_10890"
     CDS             complement(<1..917)
                     /locus_tag="PK281_10890"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOY49893.1"
                     /translation="MKKIVSLYLLLFSFSTFAQTVQLSSLQDALNLARKQNNSIRSSE
                     LQNRIAQTDNALAQSGLYPSLSLTGNSDYNLKLPVQLIPAEIFGGPAGTFKSVQFGRA
                     WNSNASAVFELPLLHPDKYAQARAGKLTREQVELEQTAQQRLALQKVTAAYLNVLVLQ
                     QALVLNMELDSSATTLYDATKARFEQQLVSRVEINRSENLKNANLQQTLLLRSNLQVA
                     RQNLCILLGLNPNSDLQISDQLSKYAELSLTNLPEIKAENRPSYQATLKAEEAARWKM
                     RQNAYAALPKLSINSKYTFANQTDRLFSNT"
     gene            1224..1670
                     /gene="msrB"
                     /locus_tag="PK281_10895"
     CDS             1224..1670
                     /gene="msrB"
                     /locus_tag="PK281_10895"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HOY49894.1"
                     /translation="MSDNSTQASNPYYSRTDTTHLNVSNAEWKKVLSLEVYEISRLKG
                     TERAFTGKYWDTDKLGNYYCAACGNALFKSDAKFASSCGWPSFYEALRTNSVRYEEDR
                     TYGMVRVEVLCGRCDGHLGHIFDDGPAPTFKRFCMNSAVLDFEPLP"
     gene            1911..2504
                     /locus_tag="PK281_10900"
     CDS             1911..2504
                     /locus_tag="PK281_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HOY49895.1"
                     /translation="MKKIIRVLLLIFYLGISPIFAQTTSWQHRLSEAAISLTQQKVQY
                     DPTYFRISYPMGDVPADKGVCTDVVIRAYRKLGKDLQQLVHEDMKAHFNLYPKRWGLK
                     KTDSNIDHRRVPNLMVFFERFGTVKPKSSNANDYVAGDIVCWDLGGGITHIGLVIEKK
                     SPDGKRSLIVHNIGAGQVMEDVLFSWKIIGHYSYGGK"
     gene            2491..3180
                     /locus_tag="PK281_10905"
     CDS             2491..3180
                     /locus_tag="PK281_10905"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HOY49896.1"
                     /translation="MAESKRDLGQVLLEAQRNAFVLFSTAKSRGLICAGKSERDLNNE
                     LYDLAFELYGIRKYWHKRIVRSGANTLFPYRENPPDLMLQEDDILFFDFGPVFEDWEA
                     DVGMTFVLGNDPVKLKMLHDVEEAWKRGKTWYEAHPDCTGAELYAFTHDLALSLGWEF
                     GGPHCGHLIGRFPHERIEGEEKVNYLHPENHIQLDNPGKTGEKRHWIYEMHLVDQARQ
                     IGAFYEAILTL"
BASE COUNT          946 a          506 c          786 g         1013 t
ORIGIN      
        1 gtgttactga ataatcgatc ggtttgattg gcgaatgtat attttgaatt gattgataat
       61 ttaggaagtg cggcgtatgc attttgacgc attttccaac gtgcagcttc ttctgctttg
      121 agtgttgctt ggtaagatgg acggttttca gcttttattt cgggaagatt tgttaagctg
      181 agttcggcgt atttactgag ctgatcgcta atttgtaaat cggaattggg atttaagccg
      241 agaagaatgc agaggttttg tcgagctact tgcagattag agcgaagcaa aagtgtttgt
      301 tgcaagttgg catttttgag attttcactt cggttgattt ctactcggct aacgagttgt
      361 tgttcgaaac gcgctttggt ggcatcatat agtgtagttg cagaggaatc gagttccata
      421 ttaagcacca aagcttgttg aagaaccaga acatttaaat aggctgcagt tactttctga
      481 agagcgagtc tttgttgagc tgtttgttcc agttcaactt gttcgcgtgt gagttttcct
      541 gcacgagctt gagcgtattt atcggggtga agaagcggta attcaaaaac ggcactggca
      601 ttactgttcc aagctcgacc aaattgaacg gatttgaaag tgccagctgg tcctccaaaa
      661 atttctgccg ggataagctg aacgggtagt tttaagttat aatcgctatt tccggttagg
      721 ctgagtgatg gatataatcc gctttgagca agagcattat cggtttgagc gattcgattt
      781 tgcaattcgg aggagcggat tgaattattc tgctttcgag caagattaag tgcatcttga
      841 agtgagctaa gttgaaccgt ttgagcgaat gtgctgaacg aaaacaacaa cagataaagg
      901 gaaactatct ttttcatatt gaaaattgcg tggcaaaggt gcatggacta atagcagcta
      961 tcaaacaggg cgcaacgagg cggaagatcg tacatcgaag caaaaaaatg catattcgtg
     1021 taaggttatt tttatagcaa cgaccagatg tgtaattgaa aacaaacagt tcaaacaatc
     1081 cggatgaatg gctgttttag attcaagaca tacaaatttt aaattttgtc tctatgcgac
     1141 tgtgtatttt ttcatttgtt ttactgatga ttttttctgg cggatgtcag agtcaatcga
     1201 atcaatcaac accaactaaa tctatgagcg acaacagcac tcaagccagc aatccgtatt
     1261 attctcgaac cgacacaact catttgaatg tgagcaatgc agaatggaaa aaagtattga
     1321 gtctggaagt ttatgagatt tctcgtttaa aaggaacgga gcgtgctttt acaggtaaat
     1381 attgggatac agataaattg gggaattatt actgtgctgc ttgtggtaat gctttattta
     1441 agtcggatgc taagtttgcc agcagttgtg gatggccaag tttttacgaa gcacttcgca
     1501 cgaatagtgt gcgatatgaa gaagatcgta cctatggaat ggttcgggtt gaagtactat
     1561 gtgggagatg cgatgggcat ttgggacata ttttcgatga tggtccggcg ccaactttta
     1621 aacggttttg catgaattcg gcggtattgg attttgagcc actgccttag tttatttgag
     1681 ctattctgat ggatgaatta attgtggaat aggtatttcc gttacatatt aattcatgtt
     1741 ggatgtacgt tattaggttc aatatttcta acggttgctt gaaagaataa actttctttg
     1801 gaaatctaaa gcctctgttt aggctgcaac agggattgaa aggagctcct gcgacttgaa
     1861 agcccggggt ttttaattgt agcttgcgga gattaaaaag cgttgcccaa atgaaaaaaa
     1921 ttattcgagt tttactgctc attttttact tgggaatttc tcccattttc gcgcaaacta
     1981 caagctggca gcatcgttta tcggaagcgg ctatttcgtt aacgcaacaa aaagttcaat
     2041 atgatcctac ctattttcgc atttcctatc cgatgggtga tgtgccagca gataagggag
     2101 tttgtacaga tgtagtaatt cgagcgtatc gaaagctggg aaaggatttg caacagttag
     2161 tacatgaaga tatgaaggcg cattttaatc tttatcccaa gcgttgggga ttaaaaaaga
     2221 ccgattctaa tattgatcat cgtcgcgtac ccaatttaat ggtgtttttt gaacgtttcg
     2281 gaacggtaaa acccaagagt agcaatgcaa acgattatgt agcaggtgat atcgtttgtt
     2341 gggatttagg cggaggaatt acgcatattg gattggtaat agaaaaaaaa tcacccgatg
     2401 gaaaacgtag cttaatagtc cataatattg gggctggaca agtgatggag gatgttttat
     2461 tctcctggaa aataataggt cattacagct atggcggaaa gtaaacgcga cttaggacag
     2521 gtgctattag aagcacagcg gaatgcattt gttttattta gtacagcaaa atcgcgcgga
     2581 ttaatttgtg ctgggaaatc agagcgtgat ttaaacaatg aattatacga tttagctttt
     2641 gaattgtatg gtattcgaaa atactggcat aagcgaattg tacgttcggg agcaaatact
     2701 ttgtttcctt atcgcgaaaa tccaccagat ttaatgttgc aagaggacga tattttattc
     2761 tttgatttcg gtcctgtttt cgaagattgg gaggccgatg tagggatgac ttttgtgttg
     2821 ggaaatgatc ccgtaaaatt aaaaatgttg cacgatgtag aagaagcctg gaaacgtgga
     2881 aagacttggt acgaagcaca tccagattgt acaggtgctg aattatatgc ttttacacat
     2941 gatttagctt tatcgctcgg atgggaattt ggaggtccgc attgtggtca tttaataggg
     3001 cgatttccac acgaacgaat tgaaggagaa gaaaaggtaa attatttgca tcccgaaaat
     3061 catatacagc ttgataatcc tggtaagaca ggggagaagc gtcattggat ttacgagatg
     3121 catttagttg atcaagctag gcaaattgga gcattttacg aggcaatatt gacattgtga
     3181 ttaattatat ttgcagctct taatgcttga tagagatgtc gcttgtgatg tgctaaataa
     3241 aaatccaata c
//
LOCUS       DAOOQW010000254         3230 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_35675_length_3230_cov_3.878740, whole genome shotgun sequence.
ACCESSION   DAOOQW010000254 DAOOQW010000000
VERSION     DAOOQW010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3230
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35675_length_3230_cov_3.878740"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(599..1453)
                     /locus_tag="PK281_10910"
     CDS             complement(599..1453)
                     /locus_tag="PK281_10910"
                     /inference="COORDINATES: protein motif:HMM:NF014997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HOY49897.1"
                     /translation="MLGFYQPISSWTHLLGALITLCTGAILIRRGWGNQIRVGSLIVF
                     MIGIILMFTMSGVYHALEPGLGRQVFRRLDYAAIFVMIAGTATPIHVIMFRGWWRWGM
                     LIYLWVVAIVGLLLTVILIDQIPEWATLSVFMALGWTALASILKAGRLYGFMAIRLAL
                     LGGLFYSIGAAIDFLRWPELVPGFIGSHEIFHVFVMLGAAAHWKLIYNWADQPTHTRL
                     VFLVKEKSENELFARAVGESIFVSATSRQELRTRVKEKLDEQFHPRLIPAKIRFRYYR
                     DVVMKMGA"
     gene            complement(1588..2481)
                     /locus_tag="PK281_10915"
     CDS             complement(1588..2481)
                     /locus_tag="PK281_10915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49898.1"
                     /translation="MLYSGDSKLKPNKGFQYFLLTAFSALLVGAGALFVHNRGMLDDL
                     RAERVSSESIIHERNFALQEIRIVKNKLRASDDLNQARIDSLADLSNQLVQALKSNKN
                     LQEKIKESEEQQTTVSAYQTLKFEQDQAFQQLEKRSGELQSQNQHLKHNLHSVEQENS
                     RLTTQLDEYVNKAILFDVEARRSLTGKLTVHARNTRAIHVEFTIAKGTLEPNSKLYVT
                     LTDIQQGKGIPGKELPITLSLDGENMTIQPAALAKWEGKGAADRMELGFDLDKKLQKG
                     IYQVDVYSDSRYLGGSRLRLQ"
     gene            complement(2538..>3230)
                     /locus_tag="PK281_10920"
     CDS             complement(2538..>3230)
                     /locus_tag="PK281_10920"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOY49899.1"
                     /translation="IEEAFQMQTQFVANASHELRTPLTVLSGQLEVALLKERTPEQYQ
                     IILTSLKEDLSLLNQTANRLLLLAQADSRAENLDARPFRLDETLWQIQSEMLKRYPGA
                     EIEIGFSRIPESEFEFMITGNEQLIRVAIGNVVENALKYSNPQRCKITFDIEHSEKSV
                     IVEDKGSGIAADEHHKIFLPFYRSPSSINAPGHGLGLAMVKKILQLHKGEITLTSQKG
                     EGTTVKISFSES"
BASE COUNT         1017 a          669 c          545 g          999 t
ORIGIN      
        1 tttgtagtat ttacggatct taccgtttac cggtttttct tctttttcaa gaagtaacaa
       61 acgtaattct ggtttctcct gaagcaaggt aaaagctgtt gctaggccaa caattcctcc
      121 acctataaca atacaatcgt gattcattca attgattttg gagcacaaaa atacatgctt
      181 taaccaccga aaactaaaaa accggcttgc ttaataaggc ttaccggttt ttaacaacaa
      241 aacacttgtc ttaatttctt tcaaatgcca cttcagggga acttataacg agttaggcac
      301 cagatcataa gttcctctgc ttcaggttcg actaagcgtt gaaatgcttt acggagttct
      361 ttgataaaca aacttgcatc aaagctaacg ttctctaaaa tgactttact gtattcgagc
      421 atcgacatag acataacaca aactattaaa acgttcaaca aagccagttt gaaacgaatt
      481 attgccggaa tattgtccgg aaatggtagt attcacaaag tttaacactt tgtcaacgca
      541 tcgtacactg gatagtaaat cgtttttcga tagtgtgatt gtctttagtt tgaattcatt
      601 atgctcccat tttcataact acatctctgt agtaacgaaa acgaatttta gcagggataa
      661 gacgtggatg aaattgttca tccagcttct cttttacccg tgtacgcaat tcttgtcgtg
      721 aggttgcaga aacgaaaata gattctccaa cagcacgtgc aaaaagttca ttttcagatt
      781 tttctttcac tagaaaaacc aaacgggtat gagttggttg atcagcccaa ttgtaaatta
      841 atttccaatg tgcagcagca cctaacatca caaatacatg gaaaatctca tgtgatccaa
      901 taaaacctgg aactaactca ggccaacgta gaaaatctat tgctgctcca atagagtaaa
      961 acaatccacc taataatgcg aggcgaattg ccatgaagcc atataaacgt cctgctttta
     1021 aaatcgatgc taatgcagtc catcctaaag ccataaagac acttaatgtg gcccactcgg
     1081 gaatttgatc tattaagatt actgtcaata aaagtccaac aattgcaact acccataaat
     1141 aaatgagcat tccccatctc caccaacctc tgaacattat aacgtgaatt ggagttgctg
     1201 tacctgcaat catgacgaaa atcgcagcat aatcgagtct acgaaaaact tgtctaccta
     1261 atcctggctc caatgcatga tacacaccac tcatggtaaa catgagaata ataccaatca
     1321 taaaaacgat gagtgatccg acacgaattt ggttacccca accacgtcga atcaatatag
     1381 cgcctgtaca taaagttatc agggctccca gtaaatgagt ccagctcgat atcggttggt
     1441 aaaatcccag cataacctga agtttaaaat catggatagt tcctatacca atccttgaat
     1501 ttaatgttgc cggaaaaaag ccacgaaact tacatctgaa aatctcttat ttcagtgcta
     1561 ctgtttcgaa taatatgttt cgaattctta ttgaagtctt aaacgagaac ctcccaaata
     1621 cctcgaatcg gaataaacat ccacctgata aattcctttt tgaagcttct tatctaggtc
     1681 gaatcctaat tccatccgat cggctgcgcc ctttccttcc catttagcta aagctgctgg
     1741 ttgaatggtc atattttcac catctaatga aagtgtaatt ggaagttctt ttccgggtat
     1801 gcctttgcct tgctgaatat cagtaagagt tacatacagt tttgaattag gctctaaagt
     1861 gccttttgcg atagtaaact caacgtgtat tgcacgtgta ttacgcgcat gtacagttaa
     1921 tttaccagta agtgaccgac gagcttccac atcaaacagt attgctttat tcacatactc
     1981 atccaattga gtagtcaaac gactgttttc ttgttcaact gaatgtagat tgtgctttaa
     2041 atgctgattt tgagattgaa gctcgcctga acgtttttct aattgctgaa aagcttggtc
     2101 ttgttcaaat ttcaacgttt gataagccga aacagttgtt tgctgctcct cactttcctt
     2161 aattttctct tgaagatttt tatttgattt taaggcttga actaattgat tgctcaaatc
     2221 agctaatgag tcaattctag cttggttcaa atcgtcagaa gcacgcaatt tattcttaac
     2281 gattctgatt tcctgcaagg caaaattacg ctcatgtata atcgattctg aactaacacg
     2341 ctcagcacgg aggtcgtcca acattcctct attatgcaca aataaagctc cagctccaac
     2401 tagtaaagcc gaaaatgctg tcaacaaaaa atactgaaaa cccttgttag gttttaattt
     2461 agaatccccc gaatataaca tatttcaaat ctgagaaaat taattgacat aaccattcct
     2521 ctcagattga aattgcatta agattctgaa aaagaaatct tcacagttgt accttctccc
     2581 ttttgagatg ttagagtgat ctcgccttta tgtaattgta aaatcttttt taccatggct
     2641 aaacccaatc catgtccagg tgcattaatg gaagaaggcg atcgataaaa tggcaagaaa
     2701 attttgtggt gttcatcagc ggctataccg gatcctttat cctcaacaat aacagacttc
     2761 tcagaatgtt caatgtcgaa ggtaatttta cagcgttgag gattactgta tttgagtgca
     2821 ttttcgacca cattaccaat agcaacccga ataagttgct cattaccagt aatcataaat
     2881 tcgaattccg attctggtat tcgagaaaat ccaatttcga tttcagctcc aggatagcgt
     2941 ttcaacattt ccgattgaat ttgccataag gtttcatcta aacgaaatgg acgagcatcg
     3001 agattttcag ctcgagaatc agcctgagcc aataatagta gcctgtttgc agtttgattg
     3061 agcaaactca aatcttcctt taatgaggta agaatgattt gatattgttc aggagttctt
     3121 tcttttaaaa gtgctacttc caactgtccg gataatactg tcaaaggtgt acgcaactca
     3181 tgagatgcat tagctacgaa ttgtgtttgc atttgaaaag cttcctctat
//
LOCUS       DAOOQW010000255         3226 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_35726_length_3226_cov_5.691580, whole genome shotgun sequence.
ACCESSION   DAOOQW010000255 DAOOQW010000000
VERSION     DAOOQW010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3226
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35726_length_3226_cov_5.691580"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1021)
                     /gene="nhaD"
                     /locus_tag="PK281_10925"
     CDS             complement(<1..1021)
                     /gene="nhaD"
                     /locus_tag="PK281_10925"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter NhaD"
                     /protein_id="HOY49900.1"
                     /translation="MLNIILAVFVLGYGAIAFEHPLKINKSGSALLMGILIWTLYRIG
                     WTSDDVAGELEHHLTGIAEILFFLMGAMTIVEVIDAHDGFNLITNRINTKSKIKLLWI
                     MAILAFFLSAILDNLTTTIVMVSLARKLIPDEKQRLFFAGMTVISANAGGAWSPIGDV
                     TTTMLWIGGQVSTLNIMKVLFFPSLVCMFIPLFYVSLFEKKTLKGDLPDRTKQKLAPE
                     NKLIFSLGLGGLILVPVFKQLTHLPPYVGMMLALGIIWIAADLIHRKKGTEEQKKYSA
                     SNALMKIDMPSLLFFLGILLAVSGLESLHVLKNVATWMDTAIGNKDLIVICIGVLSAV
                     VDNVPL"
     gene            complement(1062..1619)
                     /locus_tag="PK281_10930"
     CDS             complement(1062..1619)
                     /locus_tag="PK281_10930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49901.1"
                     /translation="MLLRAFLVIAIVAGFSSCNSTAPNKKKSTEYFDNPAFFEKEALE
                     KRASKLALFKTVESEGKSEQKRLNQVDWKKELGVFSSITLNKSSYSGKYTVDSSQQAE
                     WRQITYSTIDPKLPIKRFIVSDSSGIIHSVEAFKNESSSLVDSELHWRYVPDSGFSIY
                     GLQKVTPWSHSSFRVSAIFVSPNSR"
     gene            complement(1626..3179)
                     /locus_tag="PK281_10935"
     CDS             complement(1626..3179)
                     /locus_tag="PK281_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOY49902.1"
                     /translation="MSTATASQINISPVLKQLGIEPENTGVSTGVQWYEGSGKWIESY
                     SPVDGNLIAKVKCAGEFDYEMVIKKAEEAFKVWRLMPAPKRGEIVRQMGDALREKKDA
                     LGQLVSYEMGKSLQEGLGEVQEMIDICDFAVGLSRQLYGLTMHSERPSHRMYEQWHPI
                     GIVGIISAFNFPVAVWSWNSMLAWVCGDVCVWKPSSKVPLCSIACQNIITEVLRKNNL
                     PEGVSNLVVGNAAGDLLNNDKRVPLVSFTGSTRIGRHVSQTVAGRFGRSILELGGNNA
                     IIVTENADLKMVLVGSVFGAVGTAGQRCTSTRRLIIHESIYDKVCEVLKGAYSQLNIG
                     NPLDQNNHVGPLIDTKAVEDYSNAIEKAKAEGGKVLVEGGVLTGEGYESGCYVKPCII
                     EAENHFHIVQEETFAPILYLIKYSTIDQAIELQNGVPQGLSSSIFTNNMREMEAFLSA
                     AGSDCGIANVNIGTSGAEIGGAFGGEKETGGGRESGSDAWKAYMRRQTNTINYGTALP
                     LAQGIQFNF"
BASE COUNT          963 a          692 c          623 g          948 t
ORIGIN      
        1 caaggggcac gttatcaaca actgccgata atacaccaat acaaataacg atcaggtcct
       61 tatttccgat ggcagtatcc atccacgttg ccacgttttt aagcacgtgt aacgactcca
      121 atccgcttac tgctagaagt attcccagaa agaacaataa actcggcata tcgattttca
      181 tcaatgcatt agaagctgaa tacttctttt gttcttctgt tccttttttc cgatgaatta
      241 ggtcggctgc aatccaaata ataccaaggg ctaacatcat tcctacgtaa ggaggcaaat
      301 gtgttaattg cttgaaaacg ggaaccaaaa tcaatcctcc taaaccaagt gagaatatga
      361 gtttgttttc gggcgctagt ttttgtttgg ttcgatctgg caagtctcct tttaaggtct
      421 ttttttcgaa aaggctaacg taaaagagtg gaatgaacat gcaaactaag cttggaaaaa
      481 aaagcacttt catgatattc aaagtactta cttgaccgcc aatccacagc atggttgtag
      541 taacatcacc tataggagac caggcgccac ctgcatttgc cgatataaca gtcatgccgg
      601 caaaaaacaa acgttgtttc tcatccggaa ttagctttct cgctaaggag accatcacaa
      661 tagttgtggt aaggttgtcg agaattgctg aaaggaaaaa ggccagaatt gccattatcc
      721 acagcagttt tatcttactc tttgtattga ttctattggt tattagatta aatccgtcgt
      781 gagcatcaat tacttcaaca atggtcatag cacccatcaa gaaaaataga atttcggcaa
      841 tgcccgtgag gtgatgttct aattcaccgg caacatcatc ggatgtccag ccaattcgat
      901 ataatgtcca aatcaaaatc cccattaaca gggcagaacc agatttattt attttgagcg
      961 gatgttcgaa cgcaattgcg ccatatccaa ggacaaaaac tgccagaata atattcagca
     1021 tgttggattt ttttaaataa aaagagcctt acagaacgag gctatcgaga gttgggggaa
     1081 acgaagatag cagaaaccct aaagcttgaa tgtgaccatg gggtaacttt ttgtagtcca
     1141 taaatgctaa aaccactatc tggaacgtag cgccaatgaa gctctgaatc aactaggctt
     1201 gaggattcat ttttaaaggc ttccaccgaa tgaataatac cagatgaatc actaactata
     1261 aagcgcttaa tgggcaactt gggatctatg gtagaatagg tgatttgacg ccattctgct
     1321 tgttgagatg agtcgacggt gtattttccc gaataagacg atttgtttag tgtaatcgaa
     1381 ctaaaaacac ctaattcttt cttccagtcg acttggttaa gtcgtttttg ttcgcttttc
     1441 ccttccgatt caacggtttt aaacaatgcc aactttgaag cacgtttttc taatgcctct
     1501 ttttcgaaga atgctggatt gtcgaaatac tctgtacttt tctttttatt gggcgcagtt
     1561 gaattacagg atgaaaatcc tgcaacaatt gcaatgacca aaaaggctcg gagtagcaaa
     1621 cgaaattaga agttgaactg aattccttgt gctaaaggca gcgctgttcc gtagttgatc
     1681 gtgtttgttt ggcgacgcat atatgccttc caagcatcag aaccagattc acgtcctccg
     1741 ccagtttctt tttcaccacc aaaagcacct ccgatttcgg cgcctgaagt accaatgttc
     1801 acattagcaa taccgcaatc agaacctgca gcagaaagga atgcttccat ttcgcgcatg
     1861 ttattggtaa agattgaaga agaaagtcct tgtggaaccc cgttttgaag ttcaattgct
     1921 tgatcaatcg tactgtattt gatcaaatat aaaataggtg caaacgtttc ttcttgaaca
     1981 atgtggaaat ggttttcggc ttcaatgata catggtttta catagcaacc actttcatag
     2041 ccttctcccg ttaacacacc accttcaact aatacttttc cgccttctgc ttttgctttc
     2101 tcgatagcgt tgctgtaatc ttcaacagct tttgtatcga tcaatggtcc aacgtgattg
     2161 ttttgatcaa gaggattacc aatgtttaac tggctgtatg ctcctttaag tacttcacac
     2221 actttatcgt agatgctctc atgaataatc aagcgacgag ttgatgtgca acgttgtccg
     2281 gcagtaccaa cagcaccaaa aacagaacct accaatacca ttttcaagtc ggcattttcg
     2341 gtaacgatga ttgcgttgtt tcctcctaat tcaaggattg agcgaccaaa tcgaccggca
     2401 actgtttgag acacgtggcg accgatacga gttgatcccg tgaaagaaac aagcggaacg
     2461 cgtttgtcgt tgttaagtaa atcacctgcg gcattaccaa ctactaagtt agaaacacct
     2521 tctggtaagt tgtttttacg caatacttcg gtgatgatat tttgacaagc aatagagcaa
     2581 agcggaactt tagaagaagg tttccaaacg caaacatcac cacaaaccca tgctagcatt
     2641 gaattccaag accaaacagc aactggaaaa ttgaatgctg aaataattcc aacgattccg
     2701 ataggatgcc attgctcata catgcggtga gatggacgtt cgctgtgcat ggtaagacca
     2761 tataactggc gagatagacc aactgcaaaa tcacagatgt cgatcatttc ttgaacttca
     2821 cccaaacctt cttgaagact tttccccatt tcgtaggaaa ctaattgtcc aagtgcatct
     2881 ttcttttcgc gaagcgcatc gcccatttga cgaacaatct ctcctcgttt tggagctggc
     2941 attaatcgcc aaactttaaa agcttcttct gcttttttga tgaccatttc gtagtcaaac
     3001 tctccagcac atttcacttt agcaattaaa ttcccatcaa caggagaata agattctatc
     3061 cattttccag aaccttcgta ccattgaaca ccagttgaaa cgccagtatt ttcaggctct
     3121 attcccaatt gttttaatac tggagagata ttgatttgtg atgctgttgc agttgacata
     3181 gcaggtgaat tttgtggtgc aaaagtatga attattcagg ctcgac
//
LOCUS       DAOOQW010000256         3219 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_35827_length_3219_cov_4.257901, whole genome shotgun sequence.
ACCESSION   DAOOQW010000256 DAOOQW010000000
VERSION     DAOOQW010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3219
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35827_length_3219_cov_4.257901"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(325..3204)
                     /locus_tag="PK281_10940"
     CDS             complement(325..3204)
                     /locus_tag="PK281_10940"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOY49903.1"
                     /translation="MKNILSFLSILVLSINLSAQQFELKGRIIDKVSSEGIVGANVGL
                     YRMPDSTLITGIQTDANGNFIIPNVPGGMYRAQVTFVGYSTMRFPVRMNENKDIGTKS
                     MESTMKNLKGIEVEGAQQRAVQKGDTTEINADAFKVNPDATAEDLVKKMPGITVDNGV
                     VKAGGEEVKKVLIDGKEFFGDDASMALKNLPAEVVNKIQFFDKMSDQAQFSGFDDGNS
                     KRTMNVVTRKGMKNAQFGKIYAGGGTDDRYNAGLNMNFFKGNRRFTVLGQSNNINQQN
                     FSSQDLLGVMGSGGGGGGMGGGGMGRMMGMAPGANDPSNFMVGQSNGINTTHSLGLNY
                     SDSLSPKVKLSGSYFFNNSANATSKNIDRDYYLNDSTNQRYSESNTGWSNNLNHRLNL
                     RVEINLDTNNSIIYTPRLSWQANQNGSDVMGNTIRNAIDSISSTATNNVSRNTGYSMS
                     HNVLLRHKFKKFGRTISLTLNSDNSEKNGLSGLGSSNSFLGLIDTTITLKQQSGTYSN
                     AQNYSSNIMFTEPLGKVAQLFLNYSPTLAFNNSSKETNRLNPLSQEFTSFDTLLSNRY
                     NNTITTQKGGAGIRIRTAKLMMVFNANAQQVVLDGTQTFPQAFAIKRTFNNIVPFAMM
                     NYKFSSSTNLRVFYRSNTNVPSISQLQSVVDNSNPLQLSTGNTNLKQEYSQSISTRFA
                     SANLKSSRAFFANISSTFTNNYIANSTFLANSDTLLDGIQIRKGSQLTRPVNLNGYMS
                     VNSLLTYSLPVKWIKSTLNLQGGVNYTLKPGEINGLKNETSTITYNGGLVIASNISEK
                     IDFTLTYNGGWNQVQSTLQPTLNNNYVVQTAGFKWNWLPWKGLVLNTEINHSRYDGLG
                     TGFNQNFTLWNAGIGYKFLKGKSGELRATAFDILNQNTSISRSITETYVEDNRTKVLN
                     RYFMLTFTFTFRKFNGMKMPEVPTDPHGGPGGPGGHPMGPPPVGR"
BASE COUNT          938 a          632 c          576 g         1073 t
ORIGIN      
        1 ttcttgtctt gaaacaagaa agtaaccaaa gaagttcaag tctggacgat gctgcatacg
       61 cacaatgcct ttttccgacc attttactta gacaagcatt ttccaaatct atgtcgaaac
      121 acaccctcgc cgcccagaca tagccaacgc actgatccgt ttctaatcct aattttagtt
      181 taaataagtt aaattaaagg tattgcatat gtattgatta ttgggtttag agataaatat
      241 attaaaggat aattctaatt tttaactgca caactgatat aaaaaatccc gaaatacact
      301 agcatttcgg gatttttaat tgaattatct tccaacagga ggaggaccca ttggatgtcc
      361 acctggaccg cctgggccac catgaggatc agtaggcact tcaggcattt tcattccatt
      421 aaattttcgg aatgtaaatg tgaatgttag catgaaataa cgattcaata ctttggtgcg
      481 gttatcttcc acatacgtct cggtaatgct tcggcttatg cttgtattct gattcaatat
      541 atcgaaagct gtagcgcgta attcccctga ttttcctttc aagaatttat atccaattcc
      601 ggcgttccaa agggtgaagt tttgattaaa tccagtacct aaaccatcgt aacgagaatg
      661 gttaatttcg gtgtttaaga ctagaccttt ccacggtaac caattccatt tgaatccggc
      721 agtttgcact acgtaattgt tattcagtgt tggctgtaag gtgctttgaa cttgattcca
      781 gcctccatta taagttaggg taaaatcgat tttctcactg atattactag ctattactaa
      841 gcctccattg tatgtgattg tgctagtttc gtttttcaaa ccgttaattt caccgggttt
      901 caatgtatag tttacaccac cttgtagatt tagcgtgctt tttatccatt ttactggcaa
      961 actatacgtt aatagagaat ttacactcat gtaaccattc aaattaacag gtcgtgttag
     1021 ttgtgaacct tttctaattt gaataccatc aagaagcgtg tcactatttg ctaagaaagt
     1081 agaattagca atgtaattat tggtaaatgt gctactgata ttagcaaaga aggcccgact
     1141 acttttcaaa ttagcagaag caaaacgggt gcttattgat tgagaatatt cctgttttaa
     1201 atttgtattt cctgttgata gttgaagtgg attgctatta tctacaacac tttgcaattg
     1261 cgaaatagaa ggaacgttag tattgctgcg ataaaaaaca cgaagatttg tagaactact
     1321 gaatttgtaa ttcatcatcg caaaaggaac gatgttatta aacgtacgtt tgatggcaaa
     1381 tgcttgagga aaggtttgtg ttccatctaa tactacttgt tgagcgttgg cattaaatac
     1441 catcattaat ttagcagtac gaatacgaat accggcacca cctttttgag ttgtgatcgt
     1501 attgttatac cgattactca ataaggtatc gaaagaagta aattcctgac ttaagggatt
     1561 tagtcgattt gtttctttag atgaattatt gaatgccaag gtcggagaat aattcaaaaa
     1621 caattgagca accttaccca aaggctcggt aaacatgatg ttgctggagt aattctgagc
     1681 attactgtaa gttccagatt gctgtttaag tgtgatggtt gtgtctatta atcctaaaaa
     1741 tgaattggat gaacctaagc ctgacaatcc attcttctcc gaattatccg aattcaacgt
     1801 aagtgaaatg gtacgtccga acttcttgaa tttatgacgt aataatacgt tatggctcat
     1861 tgaatagccc gtatttcgtg aaacattgtt tgtagcagtt gaactaattg aatcaattgc
     1921 atttcggatg gtgttaccca ttacatccga accattttga tttgcttgcc aacttaatcg
     1981 tggagtgtaa ataatggaat tattggtatc taaatttatt tcaacacgta gatttaagcg
     2041 gtgatttaag ttattactcc aacctgtatt tgattcacta tatcgttgat tagtggaatc
     2101 atttaaataa taatcccgat caatattttt acttgtagca tttgcactgt tattgaaaaa
     2161 atagctaccg cttagtttta ctttcggact caatgaatca ctgtaattta agcctaagga
     2221 atgcgtggta ttaataccgt ttgattgtcc taccatgaaa ttagaagggt catttgcacc
     2281 tggcgccatt cccatcatac ggcccattcc tccgccgccc attccgccgc cgccgccgcc
     2341 gcttcccatc actcctaata aatcttggct ggagaagttt tgttgattga tgttattgct
     2401 ttgacctaaa acagtaaaac gacgatttcc tttgaaaaaa ttcatgttta aacctgcatt
     2461 gtagcggtcg tcggtaccac caccagcata aattttccca aactgagcat tcttcatccc
     2521 cttacgtgta actacgttca tggtacgctt ggaattacca tcatcaaaac cactaaactg
     2581 ggcttgatcg ctcattttat caaaaaactg aattttattt accacttcag ctggcaaatt
     2641 ttttaatgcc atggatgcat catcaccaaa aaactctttc ccatcgatga gtactttttt
     2701 cacctcttcc ccacccgctt tcacaacacc attgtcgacc gtgattcctg gcattttctt
     2761 taccaaatcc tcagctgtag cgtctggatt tactttgaat gcatcggcat ttatttcggt
     2821 tgtatcgcct ttttgaacag ctctttgttg cgcaccttcc acctcaattc ctttgagatt
     2881 tttcatggtg ctttccatag atttggtacc aatgtccttg ttttcgttca ttcgaacagg
     2941 aaaacgcatg gtagaatagc caacaaaagt gacttgagcg cgatacattc ctcctggaac
     3001 attggggatg atgaaatttc catttgcatc tgtttgaata ccagtaatta aagtagaatc
     3061 gggcattcga tataatccca cattggcacc gactattcct tcggagctaa ctttatcaat
     3121 aattcgccct ttcaactcaa actgttgcgc cgataggttt atagataaaa caaggattga
     3181 aaggaagctg agtatgtttt tcatttatgt tatcaaaga
//
LOCUS       DAOOQW010000257         3189 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_36272_length_3189_cov_6.490428, whole genome shotgun sequence.
ACCESSION   DAOOQW010000257 DAOOQW010000000
VERSION     DAOOQW010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3189
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36272_length_3189_cov_6.490428"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(289..1743)
                     /locus_tag="PK281_10945"
     CDS             complement(289..1743)
                     /locus_tag="PK281_10945"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HOY49904.1"
                     /translation="MKTISLFFTAAFALAATCVNAQGSLERKPLETFNTLVVKGAVDV
                     YVNQTNESGISASASDLTSLQYSVKNGTLEINGGSEGHIYVHTQGLSRIEVYGNSDVY
                     SSDTLKGSSLAIVNSGAGDATLLLNYTDVDVNVTGSADVKLSGTASNLKADVSGAGDL
                     RAYKMPVSYATVKLSGSGDAQINASKSIQGNVGGAGTLYHQGEPEKMEVEVTGAGEVK
                     KSNALVNGDTTRIKFGNKKIIILEEDGKSKVEIGDDVIVDGEIQNKEEEKPKKDHPQS
                     IWSGFEMAINGYMNPNNSFNMDSVNSAYSLNYNKSIAVNFNFWEVHGKILKNNIYVTT
                     GLGAEINNYRFDKNVKMLSDTTPTVAVVEAGKDYDKSKLVTGYLNAPLYLTFATNKFS
                     NGKRLSISPGVTAGWKFTSYNKRVVNENGDREKSHNHDDFNLNPFRVNASVRVGYGSF
                     ILFANYALTPLFQKNEGPDLTAFSAGIRVVGFGK"
     gene            complement(2054..>3189)
                     /locus_tag="PK281_10950"
     CDS             complement(2054..>3189)
                     /locus_tag="PK281_10950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49905.1"
                     /translation="VEGLMEFTLPAEEPVKVDFKSSLLKDEADVKEHFEHLCLAYYDK
                     SISSEEKNELDFILQQKPHWEKEFKAFGFTYFQSEPEIEFAAKASLKKQFQLEGSFDD
                     QAVKAIEGLLSEDDRRAFEQSMEGDASKLYAWKAFQRTVLVKENLEFPAKDQLYQQVG
                     GGRVMPIWTRWVAAAASIALLIGAFTLLNREEVPTGIAGIRPDTGIVVRPVKAVEQVE
                     SNEMIPVNPKVQPRESSPKRIDQEKVVPEIQLNESVADLTPKRALILEDFELPDDYLA
                     ISPEQNIRIQPLMPTAQNQPEFLSPGEFVIQKAKGLLRNKRIDVEKPIQVIKEDGLAE
                     TSYKSIERITRGNVNINREKTQTGSRVTGFSIGSLEFSRSTNR"
BASE COUNT          905 a          719 c          514 g         1051 t
ORIGIN      
        1 ttattcgtaa atgtgtattt ttcaaaggtg ctcatgaggg cttcgccgtt gcatactttc
       61 ttgtgtcaag acaagaaagt atggcgtcgc agacaaacat gaatgataaa aagacaatat
      121 gcgctaacag tcatatttca ttgcttttta gaagtaatta aattgataag tcaatcgaac
      181 aaatagtatt agttgataag ctcatctata ttcaaaatcc ataaaaaact cccgccaacc
      241 tttcggatga cgggagctca caaacacctt tctgaggatc aggtgaaatt atttaccaaa
      301 accaaccact cggatgcccg cagaaaaagc agtaagatct ggaccttcgt tcttttggaa
      361 tagtggtgtt aaagcgtagt tagcgaatag aataaatgaa ccgtaaccaa cacgaacaga
      421 tgcattaacg cggaatggat tcaagttgaa atcatcgtgg ttatgacttt tttcacgatc
      481 tccattttca ttcaccacac gtttgttgta agaagtgaat ttccaaccag ctgtaactcc
      541 aggagagatg ctcaaacgtt ttccattact gaatttgtta gtagcaaaag tcaagtataa
      601 gggagcattc aaataaccag ttaccaattt acttttatca taatcttttc ccgcttctac
      661 aacagcaacg gtaggtgttg tatctgataa catctttaca tttttatcaa atcgataatt
      721 gttaatctct gcacccaaac cagttgttac atagatgttg tttttcaaaa ttttaccatg
      781 cacttcccaa aagttaaagt tcacagcaat cgatttgttg tagtttaacg agtatgccga
      841 gttcacgcta tccatgttga agctgttatt aggattcata tagccattaa tcgccatttc
      901 aaatcccgac cagatacttt gtggatgatc ctttttaggt ttctcttctt ctttattctg
      961 aatctctcca tcaacaatta catcatcacc aatctccacc ttcgatttac catcttcttc
     1021 tagaatgata atcttcttat ttccgaactt aattcgtgtg gtatctccat tcactaatgc
     1081 attcgatttt ttaacttctc cagcaccagt tacttctact tccattttct caggctcgcc
     1141 ttggtgataa agcgttccag ctccgcccac atttccttgt atcgacttgc tagcatttat
     1201 ttgagcatct cctgatcctg atagttttac agtagcatat gatacaggca ttttatacgc
     1261 tctcaaatct ccagctcccg atacatctgc ttttaaatta ctcgctgtac cactcaattt
     1321 aacatcagcc gatccagtca cattcacatc tacatcggtg tagttcaaca ataatgtagc
     1381 atcgcctgct ccagaattaa caatcgctaa actgctacct tttaatgtat ccgaactgta
     1441 tacatccgaa ttaccataca cctcaatgcg tgaaagtcct tgtgtgtgca cgtatatatg
     1501 tccttccgat ccgccattaa tttcaagcgt tccgttttta accgagtact gaagtgaagt
     1561 taaatcactc gccgaagcac taattcccga ttcattggtt tgatttacat atacatcaac
     1621 agcacctttt actacaagtg tgttaaatgt ttctagtggc ttgcgttcca aacttccctg
     1681 agcgttcacg caagtagcag caagtgcaaa tgccgcagta aaaaagagtg atatcgtttt
     1741 cataattgaa tgttcttggt gggtttcggt cataagaaga ttattggtta ttctttgtta
     1801 caattccggt aacattttct taaaataatt cacaaaagcc tatttttatg cgcattacaa
     1861 aacaggggaa gcttcattaa acctgataca ttctttattt ggggcttccc cctgctaagc
     1921 atttcactcg tttatttctt cctgcaaggg ggcgcgtcat cgctcaattc ttcgctctac
     1981 cgctaagcca tttcattcca taaaaaaagc gcctcgttaa tatgtaacga agcgctcctc
     2041 tgctaatttt ctcttatcgg ttagttgatc tactaaattc cagcgaacca atactaaatc
     2101 ctgtaacccg acttcccgtt tgtgttttct cccggttaat attcacattg cctcgtgtga
     2161 tacgctcaat gctcttataa ctggtttcag ctaatccatc ttccttgatt acctgtatcg
     2221 gtttctctac atcaattcgc ttgtttcgaa gcaatccttt tgctttttga attacgaatt
     2281 cacccggact taaaaactct ggctgattct gagccgttgg catcaatggt tgaatgcgaa
     2341 tattttgctc gggacttatc gctaaataat catccggaag ttcaaaatct tccaatatca
     2401 aagctcgctt aggagttaaa tctgctacgc tttcatttaa ctgaatttca ggtacgacct
     2461 tttcttgatc tatgcgttta ggagagcttt ctcttggttg aactttcggg tttactggaa
     2521 tcatttcatt gctttcaact tgctcaacag ctttaaccgg acgaacaaca atacctgtat
     2581 caggacgaat acctgcaata ccagttggaa cttcttcccg attgagtagt gtaaatgccc
     2641 ctattaataa agcaatagac gctgcagctg caacccagcg agtccaaatt ggcataactc
     2701 taccgccacc aacttgctga tacaattgat ctttagcagg aaattccaaa ttttctttaa
     2761 caagtactgt tcgttgaaat gctttccaag cgtaaagttt agatgcatcg ccttccatac
     2821 tttgttcaaa agcacgacga tcatcttcac tcaacaaacc ttcaattgct ttaacagctt
     2881 ggtcatcaaa actgccttcc aattgaaact gtttcttcaa ggaagcttta gcagcaaact
     2941 caatttcagg ttctgattga aagtaagtaa atccaaatgc cttgaattct ttttcccaat
     3001 gtggtttctg ttgaagtata aaatctaact cgttcttctc ttccgagcta attgacttat
     3061 catagtaagc taaacacaaa tgctcaaaat gttcttttac atctgcctca tctttgagca
     3121 aactactttt gaaatcaact ttcacgggct cttcagcagg taaagtgaat tccattaagc
     3181 cttctacat
//
LOCUS       DAOOQW010000258         3187 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_36303_length_3187_cov_6.575670, whole genome shotgun sequence.
ACCESSION   DAOOQW010000258 DAOOQW010000000
VERSION     DAOOQW010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3187
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36303_length_3187_cov_6.575670"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..567)
                     /locus_tag="PK281_10955"
     CDS             complement(<1..567)
                     /locus_tag="PK281_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49906.1"
                     /translation="MQLSTNRFFLFLFILISLSVNSQTTISQARLQALNSSVTIRGII
                     TNGSEIGIIRYIQDPTGGLAIYSSSFASQLNIGDSIEVSGTLVEFSGLLEINPVTNLL
                     PVSVGNALPQPQVVSPSLLSEQYESELVRLNGVIFGNGGSLFAANTSYGFSSGLEQST
                     IYVRTGSPLIGQMIPFGAVDLIGICSQFN"
     gene            complement(704..2119)
                     /locus_tag="PK281_10960"
     CDS             complement(704..2119)
                     /locus_tag="PK281_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020081046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOY49907.1"
                     /translation="MTKSDFIGLLEKHLGFPPTEGQALLFRNLTEFLSKPESNRVFVI
                     RGYAGTGKTSIVRSLVKTLPLTSLRTVLLAPTGRAAKVLSAYARQPAYTIHKKIYFAQ
                     SGDLGMHFAMQQNKHRRTVFIVDEASMIGGENFETEQNLLGDLIQYVTSGDECRLILI
                     GDSAQLPPVGLEESPALDSKELRDHYFLQVDESRLTEVMRQRLESGILFNATQIRNQL
                     DAGVNTFPHLVAEGFTDVLRIGGMELEDALNSAYSKYGTDEVLVVTRSNKNANLYNKQ
                     IRARIRWQEEEISSGDHLMVVKNNYHWLPETSGPGFIANGDTMEIQRLGKRYEKGGFR
                     FADAQVRLIDYPDEPDLEVRVLLNTLDIEQASLPWPEQKKLREVIAEDYADVPTKTAR
                     MKLLKADPFLNALQVKFAYAVTCHKAQGGQWKCVFVDQGYVTEELLDRNYLRWLYTAA
                     TRAQDRLYLVNFHAGFFGDAE"
     gene            2171..3040
                     /locus_tag="PK281_10965"
     CDS             2171..3040
                     /locus_tag="PK281_10965"
                     /inference="COORDINATES: protein motif:HMM:NF024271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3822 family protein"
                     /protein_id="HOY49908.1"
                     /translation="MFHPSPLVRYADPAFTQERTRHCRLRLQVSEQELTLAVYDPLAD
                     TFPLIERYPIRKGYAQLKPQEALARILQTHALTRLEFLSVEIILITETYTLAPGALFD
                     ATKAEDLLKLNHILKPDDTLSWDRLEAQDVYFIYAYPSAWKQVIETQFPQARIRHYAS
                     ILASGLLPDSIGQTTVFVHVQDFREDIIAVVDGKLRLFNSYSFQSPEDFLYFILLAYD
                     RLELNRETVHLCLLGEVESGSAIFASCHKYIREISFKSRRSDGSVPNPEGEVDALPAH
                     FYFNLLHSGDEDY"
     gene            3027..>3187
                     /locus_tag="PK281_10970"
     CDS             3027..>3187
                     /locus_tag="PK281_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014791796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HOY49909.1"
                     /translation="MRIISGKWRGKIIQAPENLPVRPTTDMAKESLFNWMSTRIDLED
                     ARVLDLFAGT"
BASE COUNT          914 a          708 c          599 g          966 t
ORIGIN      
        1 attaaattga gagcaaattc ctatcaagtc aactgctcca aatggaatca tttgtccgat
       61 taatggagaa cctgttctaa catatatggt cgattgttca agtcctgaac tgaaaccata
      121 ggatgtattt gccgcaaata aacttcctcc atttccgaat attactccgt ttagacgtac
      181 aagctcactt tcatattgtt cactcaaaag tgatggactt actacttgag gctgaggtaa
      241 tgcatttcca actgaaactg gaagaagatt agtaacagga ttaatttcta atagtccaga
      301 aaattctact agtgtaccac ttacttcaat actatctcct atatttaatt ggctagcaaa
      361 actgctacta tatatagcta atccaccagt aggatcttgg atgtatcgaa taatacctat
      421 ttcggatcca ttagtaatga ttcctcttat tgttactgaa ctgttaagag cctgaagtct
      481 tgcttgacta attgttgttt gactgttaac agataacgaa ataagaataa agagaaaaag
      541 aaaaaatcga ttggttgaaa gttgcatacc cttgttttat tataggtttg aaagaattac
      601 ctacatgaac aaaggttatt gccttgaatc taattcaagt taattataaa ttatgcttaa
      661 caatctttaa ggttatgaat acgtatttga attgtttcct tttctattct gcatccccaa
      721 aaaatccagc atggaaattc accaaataca aacgatcctg cgcacgcgtt gccgctgtat
      781 acaaccaacg taaataattc cgatcaagta attcttccgt cacatatcct tgatctacaa
      841 acacacattt ccattgtccg ccttgagctt tgtgacacgt tactgcataa gcaaacttca
      901 cttgtaaggc atttaaaaat ggatctgctt tcaacagctt catacgagcc gtttttgttg
      961 ggacatccgc ataatcctca gctatcactt cccgtaattt cttctgctcg ggccagggta
     1021 aactagcttg ttcaatatct aacgtattta ataaaactcg aacttctaag tcaggttcat
     1081 cgggataatc aatcagtcga acttgtgcat ccgcgaagcg aaaaccacct ttctcatatc
     1141 gtttacccaa gcgttgaatt tccatggtat caccattggc aataaagccc ggaccgctcg
     1201 tttcgggaag ccagtgatag ttattcttta ccaccatcaa atggtcgccg ctgctaattt
     1261 cctcttcctg ccagcgaatt cgtgcccgga tttgtttgtt atacagattc gcattcttgt
     1321 tcgatcgggt aaccaccaaa acttcgtcgg ttccatattt cgaataagcc gaattcaagg
     1381 catcctccaa ttccatccca ccaatacgca gcacatcggt aaatccttcc gccaccaaat
     1441 gaggaaaagt attcactcct gcatccaact gatttcgaat ctgtgtagca ttaaacaaaa
     1501 tcccactctc aagccgctga cgcatcactt cagtaagccg actttcatcc acctgcaaaa
     1561 aataatgatc acgtagttct ttgctatcga gtgccggact ttcttctaat ccaacgggcg
     1621 gcaactgagc gctatcgcca atcaatatca atcgacattc atcaccgctt gttacatatt
     1681 gaatcaaatc gcccaagagg ttttgctcgg tttcgaaatt ctctccacca atcatcgaag
     1741 cctcatccac aataaataca gtacgtcgat gcttattttg ctgcatggcg aagtgcattc
     1801 ctaaatcacc actttgggca aaataaattt tcttgtgaat ggtataagcc ggttgacgcg
     1861 cataagccga taaaaccttc gctgctcggc ctgttggtgc aagtaaaact gtcctcaaac
     1921 tcgttaaggg cagcgttttt accaatgatc gaacaatact cgttttccca gtacccgcgt
     1981 agcctcgaat gacaaatact cggttcgatt caggtttact taaaaattca gtgaggttac
     2041 gaaatagcaa tgcctgccct tcagtaggag ggaagccgag gtgcttttct agcagtccga
     2101 taaagtcgct cttggtcaat ccgtggtttt ggtctaaact tgccaaagat agcgagctct
     2161 tctatatata atgtttcatc caagtccact tgttcgatat gccgacccgg cttttactca
     2221 ggaacgcacg cgtcattgtc gattgcgctt acaggtttcg gagcaagaac tcacgttggc
     2281 ggtttatgat cctttggccg atacctttcc tttgattgaa cgatatccca ttcgaaaagg
     2341 atatgcgcaa ctcaaacctc aggaagcact tgcccgtatt ttacaaacgc atgcactaac
     2401 ccgcttagaa tttctatcgg tcgaaattat cctcatcacc gaaacctata cactagcacc
     2461 gggagcttta tttgatgcaa ctaaagcaga agatttactt aagctaaatc atattctaaa
     2521 acccgacgat acgcttagtt gggatcgact tgaagcgcag gatgtgtatt tcatttatgc
     2581 ctatccaagt gcgtggaaac aagtcattga aacgcaattt cctcaagctc gaattcgtca
     2641 ttatgcttcg attttagcaa gtggattatt acccgattcg attggacaaa ccaccgtatt
     2701 tgttcatgtt caggatttta gggaagatat aattgccgtg gtggatggaa aactacgctt
     2761 gtttaatagc tactcgtttc aaagtccaga ggattttctc tatttcattt tgttggctta
     2821 cgatcgtcta gaactcaatc gtgaaaccgt tcatctatgt ttattgggtg aagtggaatc
     2881 gggttcagct atttttgctt cttgtcataa gtacattcga gaaatcagtt ttaaatcacg
     2941 acgttcggat ggttcggtgc ctaatcccga gggggaagta gatgccttac ctgctcattt
     3001 ttattttaat ttattgcatt cgggagatga ggattattag cggaaaatgg cgtgggaaaa
     3061 ttattcaggc acctgagaat ttgcctgtaa gaccaacgac cgacatggcc aaggaaagct
     3121 tgttcaattg gatgtcgacc cgaatcgatt tagaagatgc acgggtgttg gatttatttg
     3181 caggaac
//
LOCUS       DAOOQW010000259         3174 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_36513_length_3174_cov_4.361334, whole genome shotgun sequence.
ACCESSION   DAOOQW010000259 DAOOQW010000000
VERSION     DAOOQW010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36513_length_3174_cov_4.361334"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3174)
                     /locus_tag="PK281_10975"
     CDS             complement(<1..>3174)
                     /locus_tag="PK281_10975"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HOY49910.1"
                     /translation="IRIRDISAKLNNFLIIDDSLSGKISNLKFREESGFVLNKFNADF
                     IVAYTMMDFTNLIIETPNSKLDGKVHFDYDTYDDLSDFIQKVQMRGKIRKSSIDLDEL
                     AVFAPELRGLKQQISYSGGFKGTIDHLKGFNTMLSFGEDSRFIGSFIMDGLPDTEKMF
                     FHYNIAELQTSKADLEQLQMYPFHLGEKLVLPEMTQKLGLISYRGNLTGYLDDVKAEG
                     QFSTDVGDLATDLRVWYRPELDKYAYAGRFGTQEVQLGELLGLKPHLGNLAMTTEING
                     VGFNEEQLEAEINSTIPFIQLNGYTYTNITALGIVDGKTYDGRLKVNDPNLRLDFSGL
                     VDLSSEVPDFDFHAGVDRINLTALNLVQRDSALIVSTEINSKFTGKTIDQVEGQIEMA
                     QTSVLYGTQRYRMEDLLFEAVGEPEAKSIHLYSDMLDANISGAFRLASLPDAISKVLN
                     SFLPSYTALKVDEAQDFNQNFRYSVKIKDIELLSDLFFPTIKVGTGTTISGDFASMSN
                     TLNMDVEAGKLLVSGVRFEHFSTHAHAENKHLKLQAGAQHMFITDSVQVNFVQVNSDA
                     ITDSLDLNFIWASRKSLDAPDARLNARASFAGSKINLKLLPSLILIEDTLWQVNEENS
                     IVIDTGLVQFNNLSFTHQNEFVRLDGKVSERESDELDIILDNFQLRNLNPFIAESGIH
                     LEGSTRGIISLADLLGRPFFKSDLDVKNIRVNGDLIGDGLITSKWDPKTERILLDGQI
                     KAGSVPKLAFNGHYIPSKQKNNIDMQLAMNNIQLSLFKKYLEDIFSDVSGLADGAIHL
                     TGSPDEPITNGTITLKRTAVTIGLLNTRYFFAHEFNVTKNLIQAKNIQITDENSNSGK
                     LDIKISHQYFDDFYFDVFLKANSLQALNTNESQSDLFYGKANATGTFSATGPLDNIVM
                     NISAKTEKGTVFYLPLTGTSDVSQQDFITFEKKGEVKQLKKLSSRKVTSKGYELNFNL
                     EVTPDAEAFLLFDPKVGDMIKGSGSANLRMEVTEAGVFNIYGDYIIDNGDYLFTLQNV
                     INKKFVVQKGGVISFKGDPYDADINLTA"
BASE COUNT          966 a          633 c          557 g         1018 t
ORIGIN      
        1 tgcggttaga tttatatctg catcgtatgg atcacctttg aaactaataa ctccgccctt
       61 ttgaacgaca aactttttat taattacatt ttgaagggtg aataaataat ctccattatc
      121 aataatataa tccccataaa tattaaaaac tccagcttcg gtaacctcca ttcgtaagtt
      181 cgcggatcca ctacctttaa tcatatcgcc cactttcggg tcgaataata aaaatgcttc
      241 tgcatctggt gtcacctcca aattgaaatt aagttcatag cctttactgg tgacttttct
      301 agaactcaat ttcttaagtt gctttacctc gccttttttc tcaaaagtga tgaaatcttg
      361 ctgactcaca tcacttgttc ccgtaagcgg tagataaaac actgtgccct tctccgtttt
      421 ggcactaatg ttcatgacaa tattatcgag cggaccggtt gccgagaatg tacctgtggc
      481 attagctttc ccgtaaaaca aatcactttg actttcattt gtatttaaag cttgtaagga
      541 attagctttc agaaatacat caaaataaaa atcatcgaag tattggtgac taattttaat
      601 atccagtttt cctgaattgg aattctcgtc agtaatctga atattcttgg cttgtatgag
      661 gttttttgtc acgttaaatt cgtgcgcaaa gaaatatcgg gtatttaata atccaatcgt
      721 aacggctgtt cgtttaagtg tgattgttcc attggtaata ggctcatcgg gcgatcctgt
      781 tagatgtatt gctccatcag ctaaaccact cacatcggag aaaatatctt cgaggtattt
      841 cttgaataag gatagttgga tattattcat agccaactgc atatcgatgt tattcttttg
      901 cttggaagga atataatgtc cgttaaaagc taatttagga acagagcctg ctttaatttg
      961 accgtctaat aatatacgtt cggttttagg atcccattta ctagtaatta atccatcacc
     1021 aattaaatca ccatttacac gaatattctt gacatccaaa tcacttttga aaaatggccg
     1081 acccaataaa tcggcgaggg aaataattcc acgtgtagaa ccttcaagat ggatacctga
     1141 ttcggcaata aatggattca aattcctcaa ttggaaatta tcgaggatta tatcgagttc
     1201 gtctgattca cgctcactta ctttaccgtc taaacgaaca aattcgtttt gatgcgtaaa
     1261 tgaaaggtta ttgaattgta ctaaacctgt atctatgaca atagaatttt cctcattcac
     1321 ttgccataag gtgtcttcga tgaggataag tgatggtagt aattttagat taatcttgga
     1381 gcctgcaaat gaagcacgag catttaatcg agcatcagga gcatccaaac ttttacgaga
     1441 tgcccagata aaattcaaat ccaaactatc ggttatagca tccgaattca cttgtacgaa
     1501 attcacttga acactgtcgg taataaacat gtgttgtgca ccagcctgca atttcaggtg
     1561 tttgttctcg gcatgtgcat gggtagaaaa atgttcaaat cggactccag aaacgagcaa
     1621 ctttccagct tctacatcca tgtttagtgt attactcatg gatgcaaaat cacccgaaat
     1681 tgttgttcca gtacctactt taattgttgg gaaaaataaa tcagaaagga gttctatgtc
     1741 tttaatttta accgaatacc ggaagttctg attgaaatct tgcgcttcgt caactttaag
     1801 agctgtgtaa ctagggagaa agctatttaa gactttagag attgcatctg gcaaacttgc
     1861 taaacggaat gctccactga tattcgcatc taacatatcg gaatacaagt ggatcgattt
     1921 tgcttcaggc tcgccaactg cttcaaatag caaatcttcc attcggtaac gttgcgtacc
     1981 atacaaaacg gatgtttgag ccatttcgat ttgtccttct acttgatcaa tcgttttccc
     2041 ggtgaactta ctgttgattt cggtggatac gattaatgct gaatcacgtt gaactaaatt
     2101 caaggctgtt aaattgatcc gatctacccc tgcatgaaaa tcaaaatctg gcacctcgga
     2161 agaaagatca actaaacctg aaaaatccaa acgcaaattc ggatcattca cttttaatct
     2221 accatcataa gttttcccat ccacgatgcc taaagcagta atatttgtat aggtatatcc
     2281 atttaattga atgaatggaa tagtgctatt tatttcagct tctaactgtt cttcattaaa
     2341 accaacacca tttatttcgg tagtcatggc tagattacct aggtgaggtt ttaaacctag
     2401 taattcacct aattgaactt cttgcgttcc aaatcgtcct gcatatgcat atttatctaa
     2461 ttcaggtcga taccaaacac gaagatcggt tgccaaatca cccacatctg tcgaaaattg
     2521 cccttctgct tttacatcat ccaaataacc agtcaaattc ccgcggtagg aaattagtcc
     2581 cagtttttga gtcatttcgg gcaatacgag tttctctcct aaatggaaag gatacatttg
     2641 aagctgctcc aaatcggctt tagaagtttg caactcagca atgttataat ggaaaaacat
     2701 cttctcggta tcgggtaaac cgtccatgat aaagcttcca ataaaacgac tatcctctcc
     2761 aaaactcaac atcgtgttga agcccttgag atgatcaatt gttcctttaa atcctcccga
     2821 ataagaaatt tgttgtttca aaccacgtag ttcgggtgca aaaacggcta actcatccaa
     2881 atcgattgat gatttacgga ttttaccgcg catctggact ttttgaatga agtcgcttag
     2941 atcgtcatag gtatcgtaat cgaaatggac tttcccatcg agtttactat ttggtgtttc
     3001 aataatgaga ttggtaaaat ccatcatggt ataggccaca atgaaatcgg cgttgaattt
     3061 atttagtaca aaaccacttt cttctcggaa ttttagattg gaaattttcc cagaaagtga
     3121 gtcatcgata atgaggaaat tattcagttt ggcactgatg tcacgaattc gaat
//
LOCUS       DAOOQW010000260         3172 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_36549_length_3172_cov_6.224896, whole genome shotgun sequence.
ACCESSION   DAOOQW010000260 DAOOQW010000000
VERSION     DAOOQW010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3172
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36549_length_3172_cov_6.224896"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..863
                     /locus_tag="PK281_10980"
     CDS             <1..863
                     /locus_tag="PK281_10980"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOY49911.1"
                     /translation="KDKSLLNLIEQLADSEFQLSQSARNWKQDSLLYRQGAESRNSFE
                     KAQESYQYQVKKHQILTDKYQRELENSNAQIRQYAASVELKQKNLNLVRNTYEMLTIT
                     APEDGQLSAFHVEVGEYKNKGENLGQLDIQKGYKIRASIDEHYLPRVNKGQIARFDFN
                     GSTHQLQVEVIYPQVKNGRFEADLNFIQAVPSEVRKGQSIQLQLALGGSKPALQLPRS
                     AYMQKSGGNWVYVLNPDSQTAERRSIRTGRQNTDSVEILEGLSEGEQVIISSYDTFGE
                     SPTLVLKNEH"
     gene            863..1555
                     /locus_tag="PK281_10985"
     CDS             863..1555
                     /locus_tag="PK281_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013620748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOY49912.1"
                     /translation="MLIQTQQLCKIYHTEEIETTSLNDVNLSISEGEFVAIMGPSGCG
                     KSTLLNILGMLDASSSGNYVFAGTDVSSLHEKQRAEIRKNNIGFIFQSFNLIDELNVR
                     ENIELPLLYLGLDGQERKNRVDQVLEHMQIAHRAKHFPQQLSGGQQQRVAVSRAFVSR
                     PKLILADEPTGNLDSRNGEEVMRLLTELNESGTTVVMVTHSPDHAEYAHRVIQLFDGR
                     IVSENLKRSFHA"
     gene            1548..>3172
                     /locus_tag="PK281_10990"
     CDS             1548..>3172
                     /locus_tag="PK281_10990"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOY49913.1"
                     /translation="MLKQILLISWRTLLRNRLVSSITIFGLAAGITSCILIFLFVDTE
                     LSFDKGWKDADRIYRVNEVIQLQDKQDPFALTSFIVGEEIKKNVPAVEDLARLYLIGE
                     QTAWYDGKNFTVEYNYFADSSFFNILNFPFVQGDPKTALSKPKTVVISERLAQQIFGA
                     EEPMGKLIRYARNSYTVTGIIKTTEIPNHLCDVDALMSMSSMAPETRQAMSADYMQLS
                     SLTYLKVKSGIQKAVLEKQINDWSERVISPWLKENQLNGKVEFHAQLVQDIHFDTFYI
                     YDLVRKGNKTYVIIFALVAVFLLLIACFNYMNLTTAQAVKRAREVAVKKVAGASRGQL
                     ARQFLGESFFLTLIAFLLSIALVELLLPGFNQITDNDSSLLDSLQGGHFWIFILLMLV
                     LAGLAGGSYPAFYLARLQPMQVLRSNAMSTGKGLKNPGRSLGKILVILQFGISVAIIT
                     ATGVVLMQLHFLRNHELGFDKENVVSLSFPAGDTALAYKVDAMRNELKKDPNVLGFAT
                     GYHVPGKKTGRILFYYSFHGKQENQPINMSFAD"
BASE COUNT         1008 a          622 c          648 g          894 t
ORIGIN      
        1 cgaaagataa aagtttactc aacttaatcg aacagcttgc cgattcggag tttcagctta
       61 gtcaatcggc tcgaaattgg aaacaagatt cactactcta ccgacaaggt gctgaatcac
      121 gaaatagctt cgaaaaagca caagaatcct atcaatatca ggttaagaaa catcagatct
      181 taactgataa atatcaacga gaattagaaa atagcaacgc tcaaattcgc cagtacgcag
      241 catcggtcga attaaaacag aagaatttaa atttagttag aaacacatac gaaatgctca
      301 ccatcacagc tcctgaagat ggtcaattaa gtgcttttca tgttgaagtg ggtgaataca
      361 aaaacaaggg tgaaaatctc ggacaactcg acattcagaa aggatataaa attcgcgcaa
      421 gcattgatga acattatctc ccacgagtga acaaaggtca aatcgcacgt ttcgatttca
      481 atggttctac acatcaatta caagttgaag tcatctatcc gcaagtaaaa aatggacgat
      541 tcgaagcgga tttaaatttc atccaagctg taccatccga agtgagaaaa ggtcaatcga
      601 ttcaattaca actcgcatta ggaggaagca aacccgcttt acaacttcca cgctcggcat
      661 acatgcaaaa atcaggtgga aattgggtgt atgtgttaaa tccagattcc caaacagccg
      721 aacgcagatc gattcgaact ggccgtcaaa acaccgattc ggttgaaatt ctcgaaggtc
      781 tttcagaagg cgaacaagtc attatttcct cttacgacac atttggtgaa agtcccacac
      841 tagtactaaa aaacgaacac tgatgcttat acaaactcag caactctgca aaatttatca
      901 caccgaagaa atcgaaacga cctctttgaa tgatgtcaat ttatcgatta gtgaaggtga
      961 atttgttgcc attatgggac catcgggttg tggcaaatct acgctgctca atattttggg
     1021 aatgttagat gcatcaagtt ctggaaacta tgtttttgca ggtactgatg tctcctctct
     1081 ccacgaaaaa caacgtgcag aaatccgaaa aaacaacatt ggtttcatct tccaaagttt
     1141 caatctgatt gatgaattaa atgttcggga gaacatcgaa ctcccactac tctatctcgg
     1201 actcgatggg caggaacgta aaaaccgcgt tgatcaagtg ctcgaacata tgcagattgc
     1261 ccatcgtgct aaacatttcc cacaacaatt atcgggtgga caacaacaac gtgtagccgt
     1321 ttcacgagca tttgtaagtc gaccaaaact cattctagcc gatgagccaa cagggaattt
     1381 agattctcga aatggtgaag aagtgatgcg cttattgacc gaattaaatg aatcgggaac
     1441 aacggtggta atggttaccc actcgcccga tcatgctgaa tacgcacatc gagtaattca
     1501 attattcgac ggaagaattg tttcggaaaa cctaaaaaga tcgttccatg cttaagcaaa
     1561 tcctgctcat ctcttggcgg acattgcttc gaaaccggct ggtatcaagc atcaccattt
     1621 tcggtttagc cgcaggaatc acttcgtgta ttctaatatt cctgtttgtt gatacagaat
     1681 taagtttcga taaaggctgg aaagatgccg atcgaattta tcgggtgaat gaagtaattc
     1741 agttgcagga taaacaagat ccatttgcgc tgacttcttt catcgttggg gaagaaataa
     1801 aaaagaatgt tcctgcagtt gaagatttag ctcgactata tcttatcggc gaacaaactg
     1861 cttggtacga tggtaaaaac ttcacggttg aatataatta cttcgccgat tctagctttt
     1921 tcaatattct caattttcca tttgtacaag gcgatcctaa aacagcactt agtaagccta
     1981 aaacagtcgt tatctcggaa cgattagcac aacaaatatt cggtgccgaa gaacctatgg
     2041 gcaaactcat tcgctatgcc cgaaattcgt ataccgttac tggaataatt aaaacaacag
     2101 aaatacccaa tcacctctgt gatgtagatg ctttgatgtc gatgagttcg atggcgccag
     2161 aaacgcgtca agctatgtcg gccgactaca tgcaattatc gagtttgact tacttaaaag
     2221 ttaaatcggg aattcaaaaa gcagtacttg aaaaacaaat taacgattgg tcggagcgag
     2281 ttattagtcc ttggttgaag gaaaatcaat taaatggtaa agttgagttt cacgcgcaat
     2341 tggttcagga tatccatttc gacacatttt acatttatga tttagttcga aaaggcaata
     2401 aaacctatgt gatcattttt gccttggtag cagttttctt gcttttaatt gcctgtttca
     2461 attacatgaa tctcaccact gcacaagcgg tgaaaagagc acgtgaagtt gcggtgaaga
     2521 aagtggctgg agcaagccga ggacagttag ctcgacagtt tttgggtgaa tcgtttttcc
     2581 tcacactaat tgcattttta ctcagtattg cattagtaga attgttactg cccggtttta
     2641 atcaaattac tgataacgac agttctttac tggattcact tcaaggcgga catttttgga
     2701 ttttcatttt attgatgttg gtacttgctg gtttagctgg cggaagttat ccggcctttt
     2761 acttagcacg cttgcaaccc atgcaggtat tacgttccaa tgcgatgagc acaggaaaag
     2821 gactaaaaaa tccgggacgt tcattgggta aaatattagt cattcttcag ttcggaattt
     2881 cggtggcgat aattactgca actggagttg tattgatgca attacatttc ctacgtaatc
     2941 acgaattagg ttttgacaag gagaatgttg tttccttgag ttttccagcc ggagatactg
     3001 cattagctta taaagtggat gcgatgcgga atgaattgaa gaaagatccg aatgtacttg
     3061 gatttgctac tggctatcat gttccgggta aaaaaacagg gcgaattcta ttctactatt
     3121 cgtttcatgg gaagcaagaa aatcagccta tcaatatgag ttttgctgat ta
//
LOCUS       DAOOQW010000261         3164 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_36685_length_3164_cov_5.528466, whole genome shotgun sequence.
ACCESSION   DAOOQW010000261 DAOOQW010000000
VERSION     DAOOQW010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3164
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36685_length_3164_cov_5.528466"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="PK281_10995"
     CDS             complement(<1..396)
                     /locus_tag="PK281_10995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49914.1"
                     /translation="MKNANLFRLSALIMASLLSFSAGAQDGFVLDNPGKLFKKQDHRQ
                     APPLKTRYIFGVGEVSGREYLKQQDLVDPKGNVKSSGLFSEDGNKSGDLRYTYDAAGK
                     LTKTELKFIGKNIKEVTQFDANGKAVKKDI"
     gene            551..952
                     /locus_tag="PK281_11000"
     CDS             551..952
                     /locus_tag="PK281_11000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49915.1"
                     /translation="MIKDIHTPEVTDIAIAVVREAGEEGDVWNVYLVNLKDVAIESVL
                     VSSTGYGTVNDEPVKTSVLRHFLDRVGPMSFVKIEPIIEDVFVLNNEYWVSFYIDKVI
                     HDKKFIFLPGTINEAFFTSVPIVFKKGVMIK"
     gene            990..1715
                     /locus_tag="PK281_11005"
     CDS             990..1715
                     /locus_tag="PK281_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019671312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HOY49916.1"
                     /translation="MLREIPLTDILFIDIETIPAYPSFSEAPIQEQEFWKKKADTLNR
                     NQPYDVEEFYQRAGIYAEFGRVVCVSTGRLRPLTAKNNLRLEIRSFIGADENRLLREL
                     TKSLDKFNSRTQRLCAHNGKEFDFPYLSRRFLINRLELPKCLNNAGKKPWEIMHLDTL
                     DIWKFGDYKHYTSLDRLASLFGIESPKSEMDGSMVAKAWYQENLPDSIRKYCEADVLT
                     LVKVFLHIRDQSISREITVEYCR"
     gene            1865..2308
                     /locus_tag="PK281_11010"
     CDS             1865..2308
                     /locus_tag="PK281_11010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49917.1"
                     /translation="MMKNEDGLFRGVNIGDDPDQVQRAEGIAPSDFNQNVYSYDGSIG
                     ESGSYTIRYGFENGKVYEILVSATFDENAEGLNMLNGFRDYYNEVYGLYEKEGGYLVW
                     KTKSNSGNPDVMIEMVDESEFSDFGQFSLSMYHQLPPAKQVPLNQ"
     gene            2308..>3164
                     /locus_tag="PK281_11015"
     CDS             2308..>3164
                     /locus_tag="PK281_11015"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOY49918.1"
                     /translation="MSVILDINNLSVEFDSGQGVVRAVDSVNYQIQEGESVGLAGESG
                     SGKTVSAMMMLGLLPANSAKVVSGEILYTNAEGKTIDLIKISEKERRKIRGKEIGMIF
                     QEPASSLNPVLTCGEQIAETLRTHLGLSDSAAKTRTLELLQEVKLNDAARIASSFPHE
                     LSGGQKQRVMIAMAISCNPRMLIADEPTTALDVTVQKSILLLLEQLRRKYAMSLLFIT
                     HDLAVLAGLADRILIMRQGKVVEDNSIREVFLHPKNPYTQGLLSCRPTIDERPERLPT
                     VEEFLKSAHT"
BASE COUNT          951 a          640 c          653 g          920 t
ORIGIN      
        1 gatgtctttt ttaacagcct ttccattagc atcaaattga gttacctctt tgatgttttt
       61 accaatgaat ttgagttctg ttttggtgag tttgcccgca gcatcgtatg tgtaacgcaa
      121 atcaccactc ttatttccat cttccgaaaa caagccgcta gattttacat tgcctttagg
      181 gtcaactaaa tcttgctgtt tcaagtattc tcgaccgcta acttctccta cgccaaaaat
      241 ataacgtgtc ttaagaggag gggcttgacg atgatcctgt ttcttaaaca gcttgccggg
      301 attgtccaac acaaaaccat cttgtgcgcc tgctgaaaaa ctcagcaaac tcgccatgat
      361 caaggcggac aaacgaaata agttggcgtt tttcatacgc atggggcatt aattatagaa
      421 ctaaactaac tcctattttc atgtgaaaca agtaattaac aaagttttca acaagacata
      481 cggataattc tttccgtttg ttacaatacc gacagttttc cttagcctat atttaccgct
      541 ctgcagaaac atgataaaag acattcacac acccgaagta accgatattg ccattgctgt
      601 tgtgcgcgaa gctggcgaag aaggagatgt ttggaatgtg tatttggtga acttgaaaga
      661 tgtagccata gaaagtgttt tggttagttc aaccggctat ggaaccgtca atgatgaacc
      721 cgttaaaaca tccgttctcc ggcattttct cgaccgagtt gggccgatga gctttgtcaa
      781 gattgagcca attatcgaag atgtttttgt tttaaataat gaatattggg tgagtttcta
      841 tatcgacaaa gtaatacatg ataagaaatt cattttttta ccaggaacaa tcaacgaagc
      901 gttttttact tccgttccaa tcgtctttaa aaaaggtgtg atgatcaaat aggtcatcca
      961 tattcaattc tgttattaat caaatcatta tgctaagaga aattcctctg acggatattc
     1021 tatttataga tatcgaaacc attccagctt atcccagttt ttcggaagct cccattcaag
     1081 aacaggagtt ttggaagaaa aaagcggata cgctcaatcg aaaccagcca tacgatgtgg
     1141 aagaattcta ccaacgtgct ggaatatatg ctgaatttgg ccgggtagtt tgtgtttcaa
     1201 caggtaggct tcgcccattg acagccaaaa ataaccttcg attagaaatt agaagtttta
     1261 taggggctga tgaaaacaga ttgcttcgcg aactaaccaa aagcctagat aaatttaatt
     1321 cgcgaacaca acgcctttgt gctcataatg gtaaagaatt cgacttccca tatttatcga
     1381 gacggttctt aatcaatcga ctagagcttc caaaatgttt gaataatgct gggaaaaagc
     1441 cttgggaaat catgcatctc gacacattag acatttggaa attcggagat tataagcatt
     1501 acacctcgct cgatcgactg gccagtttat ttggaatcga atctcccaaa tcagaaatgg
     1561 atggtagcat ggtagcaaaa gcttggtatc aggaaaactt acccgattct attagaaagt
     1621 attgcgaagc cgatgtgctc acgttagtaa aagtcttctt acatattcgg gatcaatcca
     1681 tatcacgaga aataacagtt gaatactgtc ggtaagcagt ctgcgttcta gcatgtattt
     1741 gtgctaactt gcgacacgca tttaacctat gtctttcaaa ctctcttttt ctgcgattgt
     1801 tttgctttta gttgtttcct cttgtagggg aagacttgaa aagcacggtg aacttttcgc
     1861 cgatatgatg aagaacgaag atggcctttt tcgaggagta aatatcggcg acgatcctga
     1921 tcaggttcaa cgagcagaag gaattgcacc atccgacttc aatcaaaacg tatattctta
     1981 cgatggttct attggagaat cgggttctta taccattcgt tacggtttcg aaaacggaaa
     2041 agtgtacgag attttagtca gcgcgacctt cgatgaaaat gccgaaggtt tgaacatgct
     2101 taacggattt cgtgattatt acaacgaagt gtatggccta tatgaaaaag aaggcggcta
     2161 cctcgtgtgg aaaacaaaat ccaattctgg aaatccagat gtgatgattg aaatggtcga
     2221 cgaaagtgag tttagcgact ttggtcagtt ttcgctttcg atgtatcatc aattacctcc
     2281 agccaaacaa gttccactta atcagtaatg tcagtcattc ttgacataaa caacttaagt
     2341 gtcgaatttg actctggtca aggcgttgtt cgtgctgttg attctgtcaa ctatcaaatt
     2401 caggaaggcg aatcggtagg tttagcaggc gaatcgggct ctggaaaaac ggtatcggca
     2461 atgatgatgt tgggtttgct accagcaaat tctgcaaagg ttgtttcagg ggaaattctt
     2521 tatacaaacg cagaaggaaa aaccatcgac ttaattaaga tttccgaaaa ggaacgacga
     2581 aaaattcgtg gtaaagaaat cggaatgata ttccaagagc cggcttcttc gctcaatcct
     2641 gtattgacat gtggggaaca aatagccgaa acacttcgaa cgcatcttgg tctttcggat
     2701 tcagcagcaa agactcgaac attagagttg cttcaagaag ttaaactcaa cgacgccgct
     2761 cgaattgcat cttcctttcc ccacgaatta tctggcggtc aaaagcaacg ggtgatgatt
     2821 gccatggcaa tttcgtgcaa cccgagaatg ctcatagccg atgagcctac aaccgctcta
     2881 gatgttaccg ttcaaaaaag cattttactg ctccttgaac agcttcgccg gaaatatgcg
     2941 atgagcctac tttttatcac gcacgacctt gcggtattgg caggactggc tgatcgtatt
     3001 ttaatcatgc gacaaggtaa agttgtagaa gataattcca ttcgcgaagt gtttcttcac
     3061 cctaaaaatc catacaccca aggacttctt tcctgccgac caacaatcga tgagcgtccc
     3121 gaaaggcttc ctacagtaga ggaatttctt aaatcggcac atac
//
LOCUS       DAOOQW010000262         3161 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_36740_length_3161_cov_5.748873, whole genome shotgun sequence.
ACCESSION   DAOOQW010000262 DAOOQW010000000
VERSION     DAOOQW010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3161
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36740_length_3161_cov_5.748873"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="PK281_11020"
     CDS             complement(<1..453)
                     /locus_tag="PK281_11020"
                     /inference="COORDINATES: protein motif:HMM:NF039776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HOY49919.1"
                     /translation="MRTNCLIKLSLLFFVMLLGFIGNSYGQYCAPQYNYPCEQSTGSP
                     TADYINNFSTSNGITNIVNNNTACNGQPNNFIYYSNMTVTATQGCSFNVSMQCGSTYQ
                     QGFGIWVDWNQDLDYSDAGEYAYNSGASGFQVFNGTIQVPATALVGTTR"
     gene            complement(505..711)
                     /gene="xseB"
                     /locus_tag="PK281_11025"
     CDS             complement(505..711)
                     /gene="xseB"
                     /locus_tag="PK281_11025"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HOY49920.1"
                     /translation="MATNLTYEEAFSELKEIALAIENEAVTVDVLAEKVKRASELIGI
                     CQLKLRSTEKEINQIIKKMEGSDD"
     gene            complement(689..2134)
                     /gene="xseA"
                     /locus_tag="PK281_11030"
     CDS             complement(689..2134)
                     /gene="xseA"
                     /locus_tag="PK281_11030"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOY49921.1"
                     /translation="MMSTTQPGIRLSEFTALIRQTLEDRFAGQTFWIIAEISNLNHYA
                     RTHTFYFHLIEKEEGSGQIKAEVSAVAFSQAAYEIEGFEQLTGQVFKNGIQVLINVSV
                     NYHPVHGLKVNLNKIDASFTIGQLQQQREATLAKLLADNPDAVKLIQGRYHTRNQEVP
                     LPVVIQNIAVISSENSAGLQDFRHTLAENSFGYQFEIRLFQTLVQGDEQGKQLVDRLI
                     EIFRAEKKYDIVVIVRGGGAQTDFLMFDAYPLARAVARFPIPIITGLGHQKDVSVCDL
                     MAHTALKTPTKAAEFILAHNRNFEENILQFQHQILIRSQQILIREKERIARTNQQIQR
                     STGLLISKHSESLNSTRQKVISQSTGLVYVHARNLNLLSRQMSTRPALIVGKAKHELS
                     TYIKELQAGLTRFTQKQENYLGHYKTVFKLLSPATTLKRGFALVLDQGRILTDASSIK
                     KGDSVQVYLNGSELDVTITKKQKNNGDQFDL"
     gene            2221..>3161
                     /locus_tag="PK281_11035"
     CDS             2221..>3161
                     /locus_tag="PK281_11035"
                     /inference="COORDINATES: protein motif:HMM:NF039639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HOY49922.1"
                     /translation="MKFKESWFIVTWWLMIIASILPFLILAWFNWPASDDYFDWMIVQ
                     NQGLLDTIKSYYLNWGGRYTSYALVFLLNPLHWGENSSMCWLTFLQLGVFLYLCFGIS
                     RVLVSTNSSSTLRGLSFGLLTIFWWAYLPRPAELLYWFTGSMTYLPGLLGIYYWYHLN
                     HVERLFGLTKRVIWFALPFVIAGTNELNVVLMGLVLCWSMYDSTEDRKKFVLPAIFFF
                     LGSCFALLSPGNSGRSGFFETASGLPVHDLGFTLKQSSILAFDSISNLLFKTPVLLIG
                     AVLGFIPSETLKKKHPIGLLVIASILVPIVLYTPFCFG"
BASE COUNT          883 a          566 c          633 g         1079 t
ORIGIN      
        1 tcgggtagtt ccgactaagg ctgtagcagg aacctgaatt gtaccattaa acacttgaaa
       61 accggaagca cccgaattgt atgcatattc acctgcatca gaataatcta aatcctgatt
      121 ccaatctacc caaattccga aaccttgttg ataggttgat ccacattgca tggaaacatt
      181 aaacgaacag ccttgagtag ctgtaacagt catgtttgaa taataaataa aattatttgg
      241 ctgcccgtta catgcagtat tattattaac gatgttggtt attccgttac tggtagagaa
      301 attattaatg taatcggcag ttggactacc agtactttgc tcacaagggt aattatattg
      361 aggtgcacaa tattgcccat agctatttcc tatgaagcct aaaagcatca caaaaaacag
      421 taaagacaat ttaatcaagc agttagtgcg catcgatttg ctaagttagc atttctggat
      481 aataaacaac aggttaaatt gagattaatc gtccgaacct tccatctttt tgatgatttg
      541 atttatttcc ttttcggttg aacggagttt aagttgacaa attccaatta attcggaagc
      601 acgcttaact ttttcagcca aaacatctac cgtaaccgcc tcattttcta ttgctagagc
      661 aatttccttt aattccgaaa aagcttcttc ataagtcaaa ttggtcgcca ttattttttt
      721 gttttttagt aattgttaca tcgagttcag agccgttcaa atatacctga acagaatcac
      781 cttttttgat acttgaagca tctgttaaaa ttcgtccttg atctaaaacc aaagcaaatc
      841 cgcgttttag agtagtagcc ggacttaata atttgaaaac ggttttataa tgccccaaat
      901 agttttcttg tttttgggta aaccgagtta aaccggcttg caattctttg atataagtag
      961 aaagttcatg ttttgctttt ccaacaatta aagcaggtcg cgtactcatt tgtcgagata
     1021 gcaaattcag gtttcgagca tgaacatata ctaaacctgt actctgagaa ataactttct
     1081 gtcgagttga atttagggat tcagaatgtt ttgatatcaa taatcccgtt gacctttgaa
     1141 tctgttggtt tgtgcgtgca attcgttcct tttcacgtat taatatttgc tgagagcgga
     1201 tgaggatttg atgttgaaac tgcaagatat tttcctcgaa attcctgttg tgagctaaga
     1261 tgaattcagc agctttagtt ggtgttttta aagcagtatg agccatcaga tcacaaaccg
     1321 aaacatcttt ctgatgccct aaaccagtaa tgattggaat agggaaacgt gcaactgcac
     1381 gagcaagtgg ataagcatca aacattagaa agtctgtttg agcaccaccg ccacgaacga
     1441 ttacgacaat atcatatttc ttttccgctc gaaaaatttc aatgaggcga tcgactagtt
     1501 gtttcccttg ctcatcgcct tgtacaagcg tttggaatag gcggatttcg aattggtaac
     1561 caaacgaatt ctcggctaat gtgtgtcgga aatcttgtaa cccagcgcta ttttccgaag
     1621 aaataactgc aatgttttga atgacaacgg gcaatggtac ttcttgattt ctcgtatggt
     1681 agcggccttg aattaattta accgcatcag gattatcagc tagaagttta gctaaagttg
     1741 cttcgcgttg ttgttgcaat tgaccaatgg tgaagcttgc atcaatttta ttcagattaa
     1801 cttttagtcc gtgaacggga tgatagttta ccgaaacatt tattaatact tgaatcccat
     1861 ttttgaagac ttgtccggtt aattgctcaa aaccttctat ttcatatgcg gcttgagaaa
     1921 aagctacagc tgagacttcc gctttaattt gtcctgaacc ttcctccttt tcaattaaat
     1981 gaaagtagaa tgtgtgcgtt cgagcgtaat gattgaggtt ggagatttct gcaataatcc
     2041 aaaatgtttg tccggcaaaa cgatcttcca acgtttgacg aatcaaagct gtaaattcag
     2101 aaagacgaat tccgggttgg gttgtggaca tcatttggaa tcgtgaaaat acattaagag
     2161 cgcagtcgaa agacgattgt tgaagaattg ttgcttctac agatttgcgt tctttgtttt
     2221 atgaagttca aagagagttg gtttattgtg acctggtggc tgatgataat cgcttctatc
     2281 ctaccctttt tgatacttgc ctggttcaat tggccagcat cggatgatta tttcgattgg
     2341 atgatagtgc aaaatcaagg tttgcttgat acaattaaat cctattacct caattgggga
     2401 ggaagatata ccagctatgc tcttgtcttt ttgctgaatc ccttgcattg gggagaaaat
     2461 tcgtccatgt gctggttgac tttccttcaa ttgggagtgt tcctgtattt gtgttttgga
     2521 attagtcgag tcttagtaag tacgaattct agtagcactt tacgagggct ttcatttggt
     2581 ttattgacca ttttttggtg ggcatatttg cctcgtcctg ctgagttatt gtactggttt
     2641 acaggcagca tgacctactt accgggtttg ttgggaattt attattggta tcatttgaat
     2701 catgttgaac gtttatttgg attaacaaag cgtgtaattt ggtttgcatt gccctttgtt
     2761 attgcgggaa caaatgagtt aaatgtagtg ttgatggggt tagtgctttg ttggagtatg
     2821 tacgattcaa ctgaggatcg aaaaaagttc gttctgccag ctatattttt ctttctaggt
     2881 tcatgcttcg cacttctctc tcctggtaat agtggacgct ctggcttttt tgaaactgca
     2941 tcaggattgc ccgttcacga ccttggattt acacttaaac aaagttcaat tttagcattc
     3001 gattctattt cgaatctact atttaaaact ccggttctgt tgattggtgc tgtcttagga
     3061 tttattccat cggaaacatt aaaaaagaaa catccaattg gattacttgt aattgctagc
     3121 atattggttc caattgtctt gtatacacca ttttgctttg g
//
LOCUS       DAOOQW010000263         3152 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_36875_length_3152_cov_4.749758, whole genome shotgun sequence.
ACCESSION   DAOOQW010000263 DAOOQW010000000
VERSION     DAOOQW010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3152
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36875_length_3152_cov_4.749758"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="PK281_11040"
     CDS             complement(<1..656)
                     /locus_tag="PK281_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49923.1"
                     /translation="MKEALLLVKSINRGDRQLILTTAFAALMLSSKAYFGSASIFEPW
                     LNSESGSLQLQIADYFYTGVFASLRQLLFWAGCCVLFYLLIPMLFRRFVWPDSPSLFQ
                     LNIPRSHWKIYGYMLLFMVLPLIGASFLKSFQNTYPFVFIPQDSDFTKTLLIWEIGYL
                     FQFIAVEYFFRGFLLFSFEKHLGNKAVLIPLIPYVMIHFGKPFPETIGAILAGIVLGT
                     "
     gene            793..2283
                     /locus_tag="PK281_11045"
     CDS             793..2283
                     /locus_tag="PK281_11045"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOY49924.1"
                     /translation="MNSSPNPLSIRLFKRFLGFIFLFILGNSYAQELQILGVDSSQYP
                     NIRLSIAYKGKIRFESDQLILKQDEKNLPFTIRESAPGSAPEKGRAVFFLVEASGNTN
                     GKGLIDLREGIIGSMDNLDTKDLLNIGWFGSYDADSTNMRLITPHFTTGQANIRSLIS
                     SKLFAKPDSLNRSDLYKNIVAGLDYVSKEPDLPQNLLYIVLSTARNNSTSPLSSSDCI
                     GKAKDLGIPIFSITYLVNDSAYSSGMMTRISARTGGKNVQARTQIEIINAITDFFNAP
                     VPTSMQESVYDVLFKANTDVNPSRAKIDISFRGNRQIIVVADPNAGSLIPQDYKQYLW
                     YSIGILGLIVLIMLLINLFSRRGSRATEEEAASPEVEVETPLAIRKNVEKSIPEKKVE
                     NQVREQVESKPAGPVVLVSLQGRTTPFPLVKVETTLGRHDTNDIALPEQTVTGKHAVI
                     RMENGTITISDLGSTNGTFVNGERIRTHEIKAGDRFRLGNVELTLK"
     gene            2294..3115
                     /locus_tag="PK281_11050"
     CDS             2294..3115
                     /locus_tag="PK281_11050"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOY49925.1"
                     /translation="MKQVCRIGRNESNDLVIQDRAANDFHAIIEVNPEGLVFIRDLTT
                     HYGTFVNGKRILHCQLFPGDEVQIGFSRIDWVAHSGIPDPINELEIRPNFSVPVKQVT
                     QQVRETIQGQSEERIEKSYSGQASKVLAGVMLERESYSDRLEKELFSPQTGMELKETN
                     PSIELKPEKKIEPEEVIEEKTNEPVQAFNSEIEPIPAQEEEVPQPNLVQSEPSISLEN
                     KSETKKVDAVAQQKTTQITSTKSSKKPFSQTGQILLVLLATLLLLVAGWLLGQLS"
BASE COUNT         1042 a          610 c          616 g          884 t
ORIGIN      
        1 attgttccta atacaatacc agctaaaata gcaccaatcg tctctggaaa tggttttcca
       61 aagtgaatca tcacatatgg gataagtgga atcaggactg ctttgttacc cagatgtttc
      121 tcaaatgaaa aaagcagaaa gccacgaaag aagtattcaa cagctataaa ctgaaataaa
      181 taacctatct cccaaattaa gagcgtttta gtaaaatctg aatcctgtgg aatgaaaacg
      241 aacggataag tgttttgaaa tgatttcaaa aatgaagctc ctatgagagg caaaaccatg
      301 aatagaagca tgtatccata gattttccaa tgagaacgtg gaatgttaag ttgaaacaat
      361 gaaggtgaat ccggccaaac gaatcggcga aaaagcatgg ggattaataa gtaaaaaagc
      421 acacaacagc ctgcccaaaa tagcaactga cgtaaactag caaacactcc agtatagaaa
      481 tagtcggcta tttgaagctg caaacttcct gattcggagt tcaaccaagg ttcaaaaata
      541 ctcgcagatc caaagtatgc ttttgaacta agcatcaatg ctgcaaaggc tgtggtgaga
      601 attaattgcc ggtcgccccg gtttatactt ttgactaaca acaaggcttc cttcatcgct
      661 gtaaaaattg taaataattc tgtttataac tgcctgtagg tataaatttc aatgcctctg
      721 ttttttttgc acatttgaac gcaacggatg agtatgccag cattcgcgaa cgcctttcaa
      781 acattgtact tgttgaactc ttctccaaac ccattatcca tacgtttatt caaacggttt
      841 cttggattca ttttcttgtt tattctgggt aattcttatg ctcaagaatt acagattctt
      901 ggtgtggatt cttctcaata tcctaacatc cgcttatcta ttgcttataa aggcaaaata
      961 cgatttgagt cggatcagtt aatcctcaaa caagacgaga aaaatcttcc cttcaccatt
     1021 cgtgaaagtg cgccaggatc agcaccagaa aaagggcgtg cagttttctt cctagttgag
     1081 gcttcgggaa acaccaacgg aaaaggactt attgatcttc gtgaaggtat cattggttcg
     1141 atggacaatc tcgacaccaa agatttgctc aatattggtt ggtttggaag ctatgatgca
     1201 gatagcacca acatgcgtct tattactcca catttcacga caggtcaggc gaatattaga
     1261 tcactcattt ctagtaaatt attcgctaaa ccagattcat taaataggtc cgatctgtat
     1321 aaaaacattg tggcaggttt agattatgtt tcaaaagaac ccgatctccc tcaaaatctc
     1381 ttgtatatcg tattgagcac tgctcgaaat aacagtactt ctcccctttc ttcctccgat
     1441 tgtattggaa aagccaaaga tttaggcatt ccaatttttt ctatcaccta tttggtaaat
     1501 gatagtgctt acagttcagg aatgatgact cgtatatcag cacgaacagg tggtaaaaat
     1561 gtacaggcgc ggactcaaat tgaaatcatc aacgctatta ctgatttctt taatgcaccg
     1621 gtgcctacct ctatgcaaga atcggtgtat gatgtgcttt tcaaagctaa tacggatgta
     1681 aatccaagtc gtgctaaaat tgacattagt tttcgaggta atcgccaaat cattgtcgtt
     1741 gctgatccca acgcgggtag tttgattcca caggattaca agcaatatct ttggtattcg
     1801 attggaattt taggtctcat cgtattgatt atgttgctga ttaatttgtt ctcccgtcgt
     1861 ggatctcgtg caacagaaga agaagctgca agtccagaag ttgaagtaga aactcctctt
     1921 gcaatcagaa aaaatgtgga gaaatcaatt cccgaaaaga aagttgagaa tcaagttcgt
     1981 gagcaagttg aaagtaaacc agctggccca gtagtattag ttagtcttca gggtagaact
     2041 acaccatttc ctttagttaa ggtagaaaca acacttggtc gacacgatac gaatgatatt
     2101 gcattacccg aacaaaccgt tactggtaaa catgctgtta ttcgaatgga gaatggaacc
     2161 atcacgatta gtgatttagg tagtacaaat ggaacttttg tgaatggtga gcgtatccgt
     2221 acacacgaaa tcaaagcggg tgatcgtttc cgattaggaa atgtggaact aacgctcaag
     2281 tgaaatatca ttcatgaaac aagtttgccg aatcggacga aatgaatcga acgatctagt
     2341 cattcaagac cgggcagcaa acgattttca tgcaatcatt gaagttaatc ctgaaggatt
     2401 agttttcatt cgcgacttaa ccacccatta cggaactttt gttaacggta agaggattct
     2461 acattgtcaa ctttttcccg gagatgaagt tcaaattggt ttttcacgaa tcgattgggt
     2521 agcacattct ggaataccag atccaattaa tgaattagag attcgcccaa attttagcgt
     2581 tcccgttaag caagtcaccc aacaagtaag ggaaaccatt caaggacaat cggaagaacg
     2641 aattgaaaaa tcatacagcg gacaagcatc taaagtctta gctggtgtga tgttagaacg
     2701 tgaatcgtat tcagatcgac ttgaaaagga gcttttttct ccccaaacgg gaatggaact
     2761 caaggaaact aatccatcta ttgagttaaa acctgaaaag aagatagaac cagaagaagt
     2821 aatagaggaa aaaacgaatg agccagttca agctttcaat tcagaaatag agccgattcc
     2881 agctcaagaa gaggaagtgc cacaacccaa tttagttcaa tcagaaccta gcatttcact
     2941 tgaaaataag tctgaaacta aaaaggtaga tgctgtcgct caacagaaaa ctactcaaat
     3001 aacttcaact aagtcaagca aaaaaccgtt ttctcaaaca ggacaaatcc ttttggtact
     3061 gcttgcaact ctgttattat tagtagcagg ttggttatta gggcaattga gctaattatt
     3121 tctctgccat cgaaagcgca ccattcagat ac
//
LOCUS       DAOOQW010000264         3106 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_37606_length_3106_cov_4.621763, whole genome shotgun sequence.
ACCESSION   DAOOQW010000264 DAOOQW010000000
VERSION     DAOOQW010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3106
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37606_length_3106_cov_4.621763"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="PK281_11055"
     CDS             <1..298
                     /locus_tag="PK281_11055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49926.1"
                     /translation="LGVLRTVQDFIAPFWQFLHVQFNLEYTEIDSTFNPRRMVLRSQV
                     NTTVGGVRQSRSVFEIELNTNRISRFSCKTGSKRIQAFAVDELSELHQPSSSVN"
     gene            307..918
                     /locus_tag="PK281_11060"
     CDS             307..918
                     /locus_tag="PK281_11060"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOY49927.1"
                     /translation="MKSIILFLSLILVGFQTALATDEKELAAFKTSYSLETKSEFTKA
                     IAVMKEVYREDSYEINIRLGYLCYEAGQFTQSMTYYQNAISLKPYSIEAKLGYVLPAS
                     GLVNWDQVIVQYKKVLEIDAQNSLVNFRMGMIYYSRKDYPTAQKYFERVMNLYPFDYD
                     SVVMNAWSYLQMGKLREGKILFTKALLIRPDDSSALEGLSLIK"
     gene            975..1562
                     /locus_tag="PK281_11065"
     CDS             975..1562
                     /locus_tag="PK281_11065"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HOY49928.1"
                     /translation="MRILKKTALIVIAFIAMVLVIALFLPKTYSISRFIEINSSTEVV
                     YNQVSDFSTWKEWDPWSRNDQRVKIQLNGIPGKPGHQRHYMGGEMGEGKMTISACTPY
                     SLLISKIEVTEPATLQFEDIWTFDGTENGTKVTWTSKGDLDYPFGRLYGLMINQLLGN
                     EMADGLDSLKSYTEAMPEEPITNPHELLIEQDHAD"
     gene            1626..2252
                     /locus_tag="PK281_11070"
     CDS             1626..2252
                     /locus_tag="PK281_11070"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOY49929.1"
                     /translation="MKATEKRIQILQAAKACFTQYGYDKTTLDDIGKRLGLNKSSLYY
                     YFKNKEEIFTAVVVQETDDILVELQQEMSVHHEPEEKIQLYMRKRLGYYRKVLSLHQL
                     TAESVRQIQPGFHALIHDILQREIAFVSRELMLMDPQLSDAMVKRVAELIISSADAIK
                     HEEVIYNRLSMNDTPDYQRIEKDTELLIRLILHGVRQTVRKHYPEMAE"
     gene            2452..3009
                     /gene="msrA"
                     /locus_tag="PK281_11075"
     CDS             2452..3009
                     /gene="msrA"
                     /locus_tag="PK281_11075"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008990288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HOY49930.1"
                     /translation="MTAQPEQKTDTAYLAAGCFWCIEAIFQQLDGVISVSSGYTAGQV
                     KNPSYKEVCTGLTGHAEAARIIYDPAKLSFDELLEVFWKTHDPTTLNRQGADVGTQYR
                     SGIYYINDEQKATAEKYKKELDASGAFEKPIVTEIVPFDGAFYVAEDYHQNYYNQNGE
                     QGYCRMVIQPKVEKFKKVFPDKLKK"
BASE COUNT          976 a          614 c          632 g          884 t
ORIGIN      
        1 actaggtgtt ttgagaaccg ttcaagattt cattgctcct ttttggcagt tcctgcacgt
       61 tcagtttaat ctcgaataca ccgagattga tagcacgttc aatcctcgtc gcatggtatt
      121 acgatctcag gtaaatacaa ctgtgggcgg tgttcgtcaa agtaggagtg ttttcgaaat
      181 tgaacttaac acgaaccgta tcagtcgttt ttcttgtaaa actggttcca agagaattca
      241 agcttttgct gtggatgaac tcagcgagct acatcaacct tcttcatcag tcaactaatc
      301 aaacctatga aatctatcat cctttttcta agcctaatcc ttgttggctt tcaaactgct
      361 ctagctaccg acgaaaaaga actagctgca ttcaagacta gctattcgct cgaaacaaaa
      421 tccgaattca ccaaagctat tgctgtgatg aaagaagtgt atcgggaaga ttcgtatgaa
      481 atcaatatac gattgggcta tttatgctat gaagctggac agtttactca aagtatgact
      541 tattaccaaa atgccatcag cctaaaaccc tattcaatag aagctaaatt gggttatgtg
      601 ttaccggctt ctggattagt aaattgggat caagtaattg ttcagtataa aaaggtgttg
      661 gagattgatg cccaaaattc cttagtcaat ttccgaatgg ggatgattta ctatagtcgt
      721 aaagattatc caacagctca gaaatatttt gaacgggtga tgaatttata tccgttcgat
      781 tatgatagcg tagtgatgaa cgcttggagt tatttgcaaa tgggcaaact gcgtgaagga
      841 aaaatcctat ttacaaaggc acttttgatt cgtcccgatg atagctccgc tttggaagga
      901 ttaagcttga tcaaataatc ctaccgtata tcttctgact attttgctat ttttgaaaac
      961 ctagcaaaac aggcatgcgg atactgaaaa aaacagcact catagtcatt gcctttattg
     1021 caatggtgct agttattgca ctctttcttc caaagaccta ttcgataagt cggtttatag
     1081 aaatcaatag ctccaccgaa gttgtgtata atcaggtgag cgattttagc acgtggaaag
     1141 aatgggatcc ttggagtcgt aacgaccaac gagtgaaaat tcaactcaat ggaattcccg
     1201 gaaaacccgg acatcaacgt cattatatgg gtggtgaaat gggcgaaggc aagatgacaa
     1261 taagcgcttg tacaccttat tcgcttttga tcagcaaaat tgaagtaaca gagcctgcaa
     1321 ctttacaatt cgaagatatc tggacttttg atgggaccga aaacggcact aaagtaacct
     1381 ggactagcaa aggcgattta gattatccat ttggtcgctt gtatggattg atgattaatc
     1441 aactgttagg caatgaaatg gctgatggtt tagattcgtt aaaatcatac accgaagcta
     1501 tgcccgaaga acctatcacc aacccacacg agctactgat agaacaagat cacgccgatt
     1561 aaaacttttc gaacaatcag ttcatctgtt tgaactttca atacatttgt agcaattctt
     1621 cctttatgaa agctacagaa aaacgtatcc aaattcttca agctgcaaaa gcctgtttta
     1681 cccaatacgg ttacgataaa acaacgctgg atgatattgg taaacgccta ggtttgaata
     1741 aatcttcgct ctactactac tttaaaaaca aggaggagat ttttactgct gtggtggttc
     1801 aagaaacaga tgatattctt gtagaacttc aacaggaaat gtcggtgcac cacgagccgg
     1861 aggagaagat tcagctttat atgcgtaaac gtttgggtta ctaccgtaaa gtgttgagcc
     1921 tacaccaact tacagccgaa agcgttcgac aaatacaacc gggatttcat gctttgattc
     1981 atgatattct tcaacgtgaa attgcatttg tatcgcgcga gctcatgttg atggatcctc
     2041 aattgagcga tgccatggta aaacgtgttg ctgaacttat catttcgtcg gctgatgcca
     2101 ttaagcacga agaagtaatc tacaaccgat tgagcatgaa cgacacgccc gattatcaac
     2161 gtatcgaaaa agataccgag ttgctgattc gcctcattct tcatggtgtt cgacaaacgg
     2221 tgagaaaaca ttatcccgaa atggctgaat aaattttagg tttagtgtaa ggttggaaca
     2281 aaatctccga caaggcttaa ccatttcatc agaaatgtgt ttcatcttaa atacaagcct
     2341 atgcacctgt acaaaaaatt tagattttta atgattcttc tcgggatcag ttctggatta
     2401 caagcctgtt cttctggaac caattcaact attgctaccc aatttcctga tatgactgcc
     2461 caacccgaac aaaaaaccga taccgcttac ttagccgccg gatgcttttg gtgcatcgaa
     2521 gctatctttc aacaactcga tggagttata tctgttagtt caggatatac tgctggacaa
     2581 gtaaaaaacc cgagttataa agaagtatgc accggactaa ctggtcatgc cgaagctgca
     2641 cgtatcattt acgatccagc caaacttagc tttgatgagt tgctcgaagt attttggaaa
     2701 acgcacgatc caactacatt aaatcgacaa ggtgccgatg tgggaactca gtaccgatct
     2761 gggatttatt acatcaacga tgagcaaaaa gcaactgctg agaaatacaa aaaggagcta
     2821 gatgcatcgg gtgcttttga gaaaccgatt gtaaccgaga ttgtcccgtt tgatggagca
     2881 ttttacgtgg ccgaagatta tcatcagaac tactacaacc aaaatggcga acaaggttat
     2941 tgccgcatgg tgatacaacc gaaagtggag aagtttaaaa aggtatttcc cgataaactg
     3001 aagaaataaa tcccaagcag gagcattaaa ctcctgcttt ttttatgcgg tttgaatgct
     3061 ggttttccga cgaattagcc acaatccgaa aaaggttagt aagcca
//
LOCUS       DAOOQW010000265         3103 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_37666_length_3103_cov_4.878281, whole genome shotgun sequence.
ACCESSION   DAOOQW010000265 DAOOQW010000000
VERSION     DAOOQW010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3103
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37666_length_3103_cov_4.878281"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..823
                     /locus_tag="PK281_11080"
     CDS             <1..823
                     /locus_tag="PK281_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011710406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HOY49931.1"
                     /translation="LKERLEKELEKNLALRVQPTNSADSFLVFGRGILHLSILIETMR
                     REGYELQVGQPQVIIKEVGDVKHEPVEVLTIDVPEEYSGKVIELVSQRKGELRVMEPK
                     GDMQHLEFEIPARGIIGLRNHLLTASAGEAIVAHRFKDYEPWKGEIPQRTNGVMIAKD
                     PGKSIAYSIDKLQDRGYFFVDPGEDVYTGMIIGEQNKAGDMVVNITQEKKLTNMRASG
                     SDDKMRIAPAIKFSLEEAMEYIQKDEYVEITPSALRIRKIHLDENTRKRAAAQLS"
     gene            complement(940..1956)
                     /locus_tag="PK281_11085"
     CDS             complement(940..1956)
                     /locus_tag="PK281_11085"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY49932.1"
                     /translation="MKFFTLFAATFLTVSMSLHAQTINSDWVSSPGVNHVNHLVTLSG
                     SIDPGPAGSGQSWNYGSLVSDSMYTEFVVPPANAAGNSNFPTATVAFVSNEDVSSYNA
                     FYTVDANVWASIGAYVASTDGNILVNYTNPMDMLHFPASLNSTFTDNYAMTFTAGPLS
                     YSRTGTNVTVMDASGTLNTPAGTFSNVLRMMSVQSYVDNFGIPLPPPAGEGTTTVYNW
                     ISPDYPGVILLSITINESEGASNTSVFYVNPTEVGFDQIASSTIGIYPNPANDQVRVT
                     ADMNITRVEVMDLSGRMVTTQSISSNQSQALVSTSALNNGVYILRAVNSNGNSTSTRL
                     VVAH"
     gene            complement(2170..>3103)
                     /locus_tag="PK281_11090"
     CDS             complement(2170..>3103)
                     /locus_tag="PK281_11090"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY49933.1"
                     /translation="FSQTYNVANISGSISPGSTGASQSWNFASLSSDSVYTETYGSPA
                     AAPGGSSFPTSTVASQWTDGSIQSNNFYLVSSSLMQVVGSFTTTSFGNITISFSDPMD
                     YLRFPCNLNDAYVDSFYSSINATTGLSFQQNGRNSVTVDGSGSLTTPVGTFTNVLRVK
                     SVEEYTNFGLPPIPGSSNTGTITNYYWISSSHPGYYLMRYTIEETPTTVPDTSISYVN
                     PGQVGINDLDVADLNITPNPANELIAVNFTSGQELAGVEVLDLSGRVVAKESIIGKMN
                     IAVVSTSHLNNGMYILRATNAHGASVTTKIFIAH"
BASE COUNT         1030 a          550 c          664 g          859 t
ORIGIN      
        1 cttgaaagag cgtttagaga aggaattgga gaaaaatcta gccttacgag ttcaaccaac
       61 aaactccgct gattcattct tggtattcgg acgtggtatt cttcacttgt caatcttgat
      121 cgagacgatg cgtcgcgaag gttatgaact tcaggttgga caacctcagg taatcatcaa
      181 agaagttggt gatgtgaaac atgagccagt tgaagttttg acgattgatg taccagaaga
      241 atattcaggt aaagtgattg agttggtaag tcagcgtaaa ggtgaacttc gcgtgatgga
      301 accgaaaggc gacatgcaac atttggagtt tgagattcca gctcgtggaa ttatcggctt
      361 gcgtaatcac ttgttaacgg catcagctgg agaagcaatt gtagcacatc gttttaaaga
      421 ttacgagcct tggaaaggtg aaattccgca acgtaccaat ggagtaatga ttgcgaaaga
      481 tccaggtaaa tcgattgctt attcgattga taaattgcag gatcgtggat atttctttgt
      541 cgatccaggt gaggatgtct atactggaat gatcattggc gagcagaata aagcaggtga
      601 tatggtggta aacatcaccc aagagaaaaa actaacgaac atgcgtgcat ctggatcaga
      661 tgataagatg cgtattgctc cagctatcaa attctcatta gaagaagcga tggagtatat
      721 tcagaaagat gagtatgtag aaattactcc gagtgcatta cgtattcgta agatacattt
      781 ggatgagaat acgcgtaaac gtgcagccgc acagttatcg taatccagta ctattctaat
      841 tgaaaacgcc cgaagcagat tttgcttcgg gcgttttttt ttgtgaaaaa caaaagccgg
      901 aaaatttaaa tattccggct ctcgatcgtt tagaaaagat tagtgagcaa caactaaacg
      961 tgttgaagtt gagttgccgt ttgaatttac agcacgaagg atatatacac cattattcaa
     1021 agcggaagta gaaacaagtg cttgcgattg attagaagaa atagattgtg tagttaccat
     1081 tcgaccgctt aagtccatca cttcgacacg agtaatattc atatcggcag ttacgcgaac
     1141 ctgatcgtta gctggattag gataaatgcc aatagtagaa cttgcaattt gatcaaatcc
     1201 aacttcagta ggattcacat aaaaaacaga agtattgctc gctccttcag attcattaat
     1261 tgtaattgac agtaaaataa cgccaggata atcaggtgaa atccagttat acactgtagt
     1321 agttccttcg cctgcaggag gaggaagtgg aatgccgaaa ttgtcaacat acgattgtac
     1381 ggacatcatt cttagcacat tgctgaaagt acctgcagga gtatttagtg ttcctgatgc
     1441 atccataaca gttacattag tgcctgttcg tgaatagctt agaggacctg cagtaaaagt
     1501 catagcatag ttatctgtaa aagtggagtt caaactcgct ggaaaatgga gcatatccat
     1561 tgggttggtg tagtttacca agatgttacc atcggtagaa gctacataag caccaattga
     1621 agcccaaaca ttagcatcca cagtatagaa tgcattatac gatgaaacat cttcatttga
     1681 aacgaaggca acagttgctg tagggaagtt tgaattacct gcagcattcg ctgggggaac
     1741 aacaaactcg gtatacatgg aatcagatac taaacttccg taattccagc tctgacctga
     1801 accagcagga cctggatcaa tcgaaccact tagagttact aaatgattga cgtgatttac
     1861 tccgggtgaa gacacccagt cagaattaat cgtttgagca tgaaggctca tcgatacagt
     1921 taagaatgtg gctgcaaata gggtaaaaaa cttcatgtgt tttttctttt aaaaatagat
     1981 tagagaagcg aaactgacaa attaacttcc tgaaagaatg aacattcaaa agtgaataca
     2041 aaggctctta ctcgccttat tttcaattct atatacagat tgaaaaatca aatattccaa
     2101 gaagcgtgat tctgaacaaa aaaaccggtc cattaaatga accggttgaa atagtaactc
     2161 aagttagact tagtgtgcaa tgaagatttt agtagtaaca gaagcgccgt gtgcattagt
     2221 tgcacgaagg atatacattc cgttatttaa gtgagaagta gaaacaactg caatgttcat
     2281 tttaccaatg atagattctt tagcaactac tcgtccgctt aaatccaata cttcaactcc
     2341 agctagttct tgtccagaag taaagttaac agctataagt tcgttagctg gatttggtgt
     2401 gatgttcaag tctgccacat ctaaatcatt tataccaact tgtcccggat ttacataaga
     2461 aatagaagta tctggaactg ttgttggagt ttcttcaatt gtatagcgca tcaaataata
     2521 acctggatgg ctagaagaaa tccaatagta attagtgatt gttcccgtat tagaagaacc
     2581 tggaattgga ggcaaaccaa agtttgtgta ttcttcaact gatttaacgc gaagcacgtt
     2641 agtaaatgta ccaacaggtg tagttaacga acctgaacca tcaactgtaa ctgagtttcg
     2701 gccattttgt tgaaacgata aacctgtagt tgcattgata gaagaataaa aagaatcaac
     2761 ataagcatca ttcaaattac aaggaaaacg gaggtaatcc attggatctg aaaagctaat
     2821 ggtaatatta ccaaagcttg tcgtcgtgaa acttccaact acttgcatca aactagatga
     2881 aactaaatag aagttattgg attgaataga accatcagtc cattgcgaag caaccgttga
     2941 agttgggaaa ctcgatcctc ctggtgcagc tgccggactt ccatatgttt cggtataaac
     3001 cgagtcggaa gatagagatg caaagttcca gctttgagaa gcacctgtac ttcccggact
     3061 tattgagcca ctaatattag caacattata tgtttgagaa aac
//
LOCUS       DAOOQW010000266         3091 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_37870_length_3091_cov_4.089592, whole genome shotgun sequence.
ACCESSION   DAOOQW010000266 DAOOQW010000000
VERSION     DAOOQW010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3091
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37870_length_3091_cov_4.089592"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..793)
                     /locus_tag="PK281_11095"
     CDS             complement(<1..793)
                     /locus_tag="PK281_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007646563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOY49934.1"
                     /translation="MAEEMLEDDKSKNGAYGADSIQVLEGLEAVRKRPSMYIGDTGVR
                     GLHHLVYEVVDNSIDEALAGYCKNIQVQINEDNSITVQDDGRGIPVEMHQKEKRSALE
                     VVMTVLHAGGKFDKDSYKVSGGLHGVGVSCVNALSTMLHVTVERDGKKYEQEYSIGKP
                     LYNVKEIGTSSKTGTRVHFMPDDSIFTSTVYNYSTLANRMRELAYLNKGIRIQLTDLR
                     ELNEQGEPSTEEFYSEGGLREFVDYLDATREKLIDEPIYMEGEKQG"
     gene            1070..2461
                     /locus_tag="PK281_11100"
     CDS             1070..2461
                     /locus_tag="PK281_11100"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOY49935.1"
                     /translation="MDRRTLLRHLLLGLPVGMALPTLLSSCKDDDFIDGAQFQGKVII
                     IGAGASGIYAAHLLLKYGIDVTVLEASNKTGGRIQSNNSFVDITLEMGAEEIHGRKSV
                     LYDLAQYFAPSRLNEVTGDDYYWISNQLRTEKFIIEAEELNGGGATLFNILESYGSYP
                     GSDMLLSEYLTAFPLDPRLELIANALTGNEYGSSNDRIGMLALKEAEAAYSSGLENFN
                     FKTGTYWNLFETAFADAINTVTLNAPVTSIDYSGNQVLVKTNNGTEYQANRVLVTVPL
                     TMLKNNSIGFVPALPSNKLQAISKIDMGIGIKITLQFSAAFWLDGTSSIVGGLKVPEY
                     WVTSNGKDTGFSYLTAFLMGPSAEELALLSHQQAVQDIIAELVTMFPSGAVASKFTGA
                     FVYKNWADEPFIGGAYSFPSITSTGQREILASPVNSKVYFAGEATNYNGHLATVHGAM
                     ESGFRAVKEILQA"
     gene            2458..>3091
                     /locus_tag="PK281_11105"
     CDS             2458..>3091
                     /locus_tag="PK281_11105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49936.1"
                     /translation="MKKLLILSCLFGISFLTSAQNYLHLNGGYSIMNPKEWNKTIATY
                     NFTRPWLKNELTTLHRDWSYGLGYSGVIARGMFISPEANYTKFTSQSTNENINTTIKL
                     NWLRGSLYLDLYPLEFNLDSVGFKTRPFVRLGGGATCLLPRITQNDSLTTVDDEPYNP
                     VIWSYQLTGGVGCRFYLAKHIDLIPMVLYHYQPKVNLEDFSYALHGSRLPG"
BASE COUNT          907 a          611 c          582 g          991 t
ORIGIN      
        1 ttccttgttt ttcaccttcc atgtaaattg gttcgtcgat caacttttcg cgtgtagcat
       61 caagataatc aacgaattcg cgcaaaccac cttctgagta aaactcttca gttgaaggct
      121 caccttgctc attcaattca cgtaaatcag tcaattgaat acgaataccc ttattcaaat
      181 atgctaactc gcgcatacga ttggctaggg tagaatagtt gtaaacagta cttgtgaaaa
      241 tactatcatc cggcataaag tgaacacgag taccagtttt agaactagta ccaatttctt
      301 tcacattata caatggcttt ccaatggaat attcttgttc gtatttctta ccgtcacgct
      361 ctaccgtaac atgtaacatg gtcgaaaggg cgttaacaca acttacccca acaccatgca
      421 aacctcctga taccttgtag gaatccttgt cgaatttccc tccagcatgt aaaacggtca
      481 ttactacctc caaggcagat cgtttttcct tttggtgcat ttccaccgga ataccacgac
      541 catcatcctg aacagtaatc gaattatctt catttatctg aacttggata ttcttacaat
      601 atcctgccag cgcctcatcg atcgagttat ctactacctc gtaaaccaaa tggtgtaaac
      661 cacgaacacc ggtgtcgcca atgtacatgg acggacgttt acgaacggct tcaagtcctt
      721 ctagaacctg aatactgtcg gcaccatatg caccgttttt cgatttatcg tcttccagca
      781 tctcttctgc catattcttt tttctaattg atcagtagga atcgtattgc tacgtgtgat
      841 agctttgttt aaagccgtgc aaatatagca aaatcgggca tattaggact atgaatcaag
      901 cagtcaaacg cttatttttt cacagataat taacattcct gattcggttt atttaataac
      961 tcattttcaa gctttgggat tggtttattc aacaaaacac tgtgtgaaat ctttgtgttt
     1021 gattgttaga atactaacca agcaacttaa attgggataa aatttcagca tggataggag
     1081 aacacttctt aggcatttgc tactcggatt accagtaggt atggcattac caactttgtt
     1141 gtcttcatgt aaggatgatg atttcattga tggagcccaa tttcaaggga aagttatcat
     1201 cataggagct ggtgcatctg gtatttatgc tgcgcatttg cttttgaaat atggaattga
     1261 tgttaccgtt ttggaagctt ccaataaaac aggcggaaga attcaatcta acaactcctt
     1321 tgtcgacatt actttggaaa tgggtgcaga ggaaattcat ggacgtaaat cggttctgta
     1381 tgatttagct caatattttg caccttctcg tttaaacgaa gtgacaggtg atgattatta
     1441 ctggattagc aatcaattac gaactgagaa atttataatt gaagcagaag agttaaatgg
     1501 aggcggtgct acattattca acattttaga aagctacggt tcctatccag gtagcgatat
     1561 gttattgagt gaatatttga cagcttttcc tttagatcct agattagaat taatagccaa
     1621 tgctcttact ggtaacgaat atggtagcag taacgaccga attggaatgt tagcattgaa
     1681 ggaagctgaa gcagcgtatt cttctggcct agaaaatttc aattttaaaa caggcactta
     1741 ctggaattta tttgaaacag cttttgcaga tgcaatcaac actgttacat taaatgcacc
     1801 tgtgacatct atcgattatt cgggtaatca agtgcttgtc aaaacgaata atggtaccga
     1861 atatcaagct aatcgtgtat tagtaactgt acccttgact atgttaaaaa acaatagcat
     1921 cggtttcgtt ccggctttac caagcaataa acttcaagct atttccaaga ttgatatggg
     1981 gattggtatc aaaatcactt tacagttttc agcagctttt tggttagatg gaacaagttc
     2041 aatagttggt ggattaaaag tcccagaata ttgggtcact tcgaacggca aggacactgg
     2101 tttttcttat ctgacagcct ttttgatggg gccttctgcc gaagaactag ccttattatc
     2161 tcaccaacaa gctgtacaag atatcattgc tgaacttgtt actatgtttc cttctggtgc
     2221 agtagcaagt aaattcacgg gggcatttgt atataagaat tgggctgatg aaccttttat
     2281 tggtggtgca tattcgtttc cttcaatcac tagtacaggt caaagagaaa tcttggcttc
     2341 accggtaaat tctaaagtat attttgcggg agaagcaact aattacaatg gacatttagc
     2401 taccgttcat ggagctatgg aatcgggttt tagagctgtt aaagaaatac tgcaagcatg
     2461 aaaaaactac tgattttatc ctgtttgttt ggaatttctt tcctcacttc agcccaaaat
     2521 tatctgcatc taaatggtgg atattccatc atgaatccaa aagagtggaa caaaactatt
     2581 gctacttaca acttcactcg accttggctt aagaatgaac tcacaacttt acatcgggat
     2641 tggtcctatg gtttaggata ttcgggagtt atagctcgag gaatgtttat ttctcccgaa
     2701 gccaattaca ctaagtttac ttcacaatcc accaacgaga acatcaacac aacaattaaa
     2761 ctcaactggt tgagaggttc tctttatctc gatttatatc cgctggaatt caatctcgac
     2821 agcgttggat ttaaaacacg accatttgtt cgattaggtg gtggcgcaac ttgtctgttg
     2881 cctcgaatca ctcaaaacga ttcacttaca actgtcgatg atgaacctta caatcctgtt
     2941 atttggtcat atcaactaac tggtggtgtt ggttgtcggt tttatttggc caaacatatt
     3001 gatctaatcc caatggttct ttatcattat cagcctaaag tcaatctgga agacttttcc
     3061 tatgcacttc atgggagtcg attgcctgga t
//
LOCUS       DAOOQW010000267         3065 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_38341_length_3065_cov_3.662791, whole genome shotgun sequence.
ACCESSION   DAOOQW010000267 DAOOQW010000000
VERSION     DAOOQW010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3065
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38341_length_3065_cov_3.662791"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1941
                     /locus_tag="PK281_11110"
     CDS             <1..1941
                     /locus_tag="PK281_11110"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY49937.1"
                     /translation="SSASFAQKAQIGEPSKALKNIKMKRPVHNHLRTSELPLFPVQAI
                     NPTVASNPGRSFGPEVVIGQTMYDLQTNNSVNYRIENFGDGTLSAIWTFSNQSTSWTD
                     RGMAYHHFDGTAWTKNPAYQDAGNITRIEELRTGFGSLARVANVGDIVVSHETAINAL
                     HVSRNTSLTDEAWSTSNNSHAEMPLIWPRMRTGGPDGKTVHVIAVTEPSGGTFTGTPY
                     YGINGCMLYNRSTDGGATFDKLMVQLPGVDSTIFASFGGDSYAMDVKGNTVAIVAGDL
                     TSRVQMWKSTDNGETWTSRTVLPFQYEPWSDATLTDFDGNGTVDSFLVDTANNVYELE
                     RIETSDGSFAIVIDNNDKVHVYYGLGYMSNDDVTDGNVSYYPGANGIMYWNEDFAVDS
                     LPQLIGGTVDRDGDSLVDVSIYFTGGTTVPYGSGMSGFPSAGVDADNTLYLSYAAYIE
                     GIDYYYLGTGPSFKHLFLQKSTDGGATWTDPKDIVGDELSGFDQLAEYQYASIARNVD
                     NNVHIVYQKDYTPGSAVTIDNAATHPFDIPNDIVYMSVTKNFDQVGIAKVNSNVNGLA
                     VMPNPASDLATLKFDLNSSENVSVNVVNMLGQRVMNLNSQHVAAGQHSISLSTNQLHA
                     GIYLVNVTAGSTSSSVKMIVKH"
     gene            complement(2120..2830)
                     /gene="bshB1"
                     /locus_tag="PK281_11115"
     CDS             complement(2120..2830)
                     /gene="bshB1"
                     /locus_tag="PK281_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013552091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB1"
                     /protein_id="HOY49938.1"
                     /translation="MKLDILAFGAHPDDIEISAGGLIISEVLQGKKVGLIDLTRGEMG
                     TRGTPEIRDEESRVAAEIMGVSVRENLGLADGLFEINHENTFKVIDAIRRYQPDIVIT
                     NAPHDRHPDHGRAATLVAEACFLAGLFKMQKEGVETQAWRPRAIFQYIQFLHHTPHFI
                     YDISEVLETKIAAIKAHKSQFWNPESTEPQTLIASEHFFQNLTSRASENGIQSGFKFG
                     EPFLTVRFPGVQSLEVLR"
BASE COUNT          911 a          583 c          646 g          925 t
ORIGIN      
        1 tccagcgcta gcttcgcaca gaaagcgcaa attggagagc cgagcaaagc actcaaaaac
       61 atcaagatga agcgaccggt tcataatcac cttcgcacat cagaattacc acttttccct
      121 gttcaggcaa tcaatccaac agtggcaagt aatccgggac gatcattcgg tccagaagtg
      181 gttattggtc aaactatgta cgatttacaa acgaacaatt cggtgaacta ccgtattgag
      241 aattttggag atggtacttt atctgccatt tggacattta gtaatcaatc tacttcttgg
      301 acagatcgtg gaatggctta tcatcatttt gatggtactg cttggacaaa aaatcctgct
      361 taccaagatg caggtaacat tacacgaatt gaagaacttc gtacaggttt tggttctttg
      421 gctcgtgtag ctaacgtagg agatatcgtt gtttctcacg aaacagcaat caatgcatta
      481 catgttagtc gtaacacttc attaacagat gaagcatggt ctacatctaa taactcacat
      541 gcagaaatgc cattaatttg gccacgtatg cgtacgggtg gtcctgatgg aaaaacagtt
      601 cacgtaattg ctgttacaga accttctgga ggaacattta caggaacacc ttattatggt
      661 attaacggat gtatgttata caatcgttca actgatggtg gagctacttt cgataaatta
      721 atggttcagc ttccaggtgt tgattcaacc attttcgcaa gttttggagg tgattcttat
      781 gcgatggatg tgaaaggaaa cacagtagca attgttgctg gtgatcttac ttcacgtgtt
      841 caaatgtgga aatcaacaga taatggtgaa acttggacta gccgtacagt tcttccattt
      901 cagtatgagc cttggagcga tgcaacatta accgattttg atggtaatgg tacagtagat
      961 tcatttttgg tagatactgc aaacaacgtt tatgaattag agcgtattga aacaagcgat
     1021 ggttctttcg caatcgtgat tgataacaac gataaagttc atgtatacta tggtcttggt
     1081 tatatgtcga atgatgatgt aactgacggg aatgtatcat actatccagg tgcaaatggt
     1141 atcatgtatt ggaacgaaga cttcgctgtt gattcacttc cacaattaat cggtggaact
     1201 gttgaccgtg atggagatag tttagttgat gtttcgattt actttactgg tggtacaact
     1261 gtaccttacg gttctggtat gtctggcttc ccaagtgctg gtgttgatgc agataatact
     1321 ctttaccttt cttatgctgc atacatcgaa ggtatcgatt actactactt aggaactggt
     1381 ccttcattca aacacttgtt cttacaaaaa tcaactgatg gtggtgcaac ttggacagat
     1441 cccaaagaca tcgttggaga tgagctttca ggtttcgatc aattagctga atatcaatat
     1501 gcttcaattg cacgtaatgt agataacaac gttcacattg tgtatcaaaa agactacaca
     1561 ccaggttcgg ctgtaactat tgataatgcg gcaactcacc cttttgatat tccgaatgat
     1621 atcgtttaca tgtcggttac caaaaatttc gatcaagtag gtattgctaa agttaattca
     1681 aatgtgaacg gattggctgt tatgcctaat cctgcatctg atttggcaac attgaaattc
     1741 gaccttaaca gcagcgaaaa tgtttctgtt aatgtggtta atatgcttgg tcaacgtgtt
     1801 atgaatttga atagtcaaca tgtagctgct ggacaacaca gcatctcttt atcaactaat
     1861 caacttcatg caggtattta tttagttaac gtaactgcag gttctactag cagcagcgtt
     1921 aaaatgattg ttaagcacta agctaaacat atcctcataa gaaagccgga ctgttatgca
     1981 gtccggcttt tttattggct ataatccaag aaaattgaat agaattatat acacacgttc
     2041 tattcgaaca aatatgcgat aagctttctc aaagacatga tgaaagctga gaattaaaag
     2101 gggaaatcga gtgaaatgac tagcgtaaaa cctctagact ttgaacacca ggaaatcgaa
     2161 cagttagaaa aggttctccg aacttaaaac ccgattgaat accgttttca gaagcccgag
     2221 aagtgagatt ttggaagaaa tgctccgaag caatgagtgt ttgcggctcg gttgattctg
     2281 gattccagaa ttgtgatttg tgtgctttaa ttgcagcaat tttagtttcg agtacttccg
     2341 atatatcgta tatgaaatgt ggagtatgat gtaaaaattg aatgtattgg aatattgctc
     2401 gaggacgcca agcttgtgtc tcaactccct ctttttgcat cttaaacaaa cctgccaaaa
     2461 aacacgcttc agctactaaa gttgcggcac gaccatgatc cggatggcga tcgtgtgggg
     2521 catttgttat gacaatatcg ggttgataac gacgaatagc atcaattact ttaaaggtat
     2581 tttcgtgatt gatttcgaaa agtccatcag ccaatcctaa attctctctc actgaaacac
     2641 ccataatttc ggcagctaca cgcgattcct catcccgaat ctcgggtgtg ccacgtgtcc
     2701 ccatctctcc tcgagttagg tcgattaatc ctactttttt gccttgtagc acctccgaaa
     2761 taatcaatcc gccagcacta atttcaatgt catccggatg tgctccgaaa gcgagaatat
     2821 cgagtttcat gggtaaatat tggattgcaa agtaaatgaa ttagaacgag tagcttgagt
     2881 ccttttttgt acgtttggag ccatgaatat atccttcttt ggagcagccc gaaccgttac
     2941 cggtagcaag catgtaatca gttatggtaa aggaaaaaaa gtactgctcg actgcggact
     3001 ttttcaaggt cacggacaag acagcgacga gtggaatcga tcctttgggt ttgatccgat
     3061 gagta
//
LOCUS       DAOOQW010000268         3012 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_39295_length_3012_cov_5.940480, whole genome shotgun sequence.
ACCESSION   DAOOQW010000268 DAOOQW010000000
VERSION     DAOOQW010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39295_length_3012_cov_5.940480"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="PK281_11120"
     CDS             <1..205
                     /locus_tag="PK281_11120"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY49939.1"
                     /translation="NEQINILRSGSAKATLELSDVTGKIVLSSLLVDKVNTINTTQLA
                     TGTYMATIRQNGSVKTVKVVVTH"
     gene            525..2459
                     /locus_tag="PK281_11125"
     CDS             525..2459
                     /locus_tag="PK281_11125"
                     /inference="COORDINATES: protein motif:HMM:NF025486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49940.1"
                     /translation="MNLRLSILFLLFSALHASAQFGTSNTSTGSMKSGFDSSGVSRDT
                     SRVELAGLELSETGVRKLQRITYSLDRFQFYLPVYQSQYINSTIGNNGTAIQNLNFRP
                     TFNSGFNWGFNSFSAFTLDLKDLQFYDAQSPYTEAAYTQGGKQEAFFKIRHIQNVGKS
                     FNFGLEFQRVNSEGTYLRQTAAHSALRFNFWYRPGKERYQILAGINYHKGASFENGGI
                     TAEGDSTFLTNTQSNRQLYAVNLVKARNKIFKNGFLVRQTYDLVQVKKDSSGTVLNPA
                     ILRLQNTFQYTLYRHSYDDENPNNSYYSTILDTNRTFVDYYNPQWENEIALLKLSALE
                     DSAQQMHWEAKAFLKQQGAHVWMEIPVNGINSRIKIFNQSVGGKINYHFRPAFSVQGS
                     TELFFAGYNQGDISLSGSIHLKPSNNWGFIAGVESFSQEPVYQLRKFDSNFGSWDNNS
                     FKKINLLRVFGTISLDRIHTDLSVSNQLISNWVFMGLNANPEQANSVLNVLRAEVSNK
                     IRVQKWYLYSRILIQQTNGNSVLSLPLVQYQESIFREGKIRKTTPWRIGIDLIGCTSF
                     QANAYQPQSGLFYLQNQKKNSGLFQANFYISAKIRRARIFAMLEHANFGIANNQSYVI
                     PYYPLPDRLIKVGFNWVFFD"
     gene            2509..>3012
                     /locus_tag="PK281_11130"
     CDS             2509..>3012
                     /locus_tag="PK281_11130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49941.1"
                     /translation="MRSILTTLIICLTSLQAFSQVQPGGIAFLSMQCDAPDGFAIVTL
                     QDIPAGTTISFTDNGWSGSALFTNENTVIWTAPATDVPIGTVIIFREDTISGAGHNTE
                     LITGPGTATGELAGLSIDGDQILAYTGTSANPTFVAAISTNMFLSTCNITGSGNSNYT
                     CLPDPLEE"
BASE COUNT          927 a          601 c          577 g          907 t
ORIGIN      
        1 gaatgagcaa atcaacatcc tacgaagcgg ttctgctaaa gctactcttg agcttagcga
       61 cgttaccgga aaaattgtat tgagctctct gcttgtcgat aaagtgaaca ccattaacac
      121 cactcaactc gcaactggta cttacatggc aacaattcgc cagaacggtt cagtaaaaac
      181 agttaaggtt gtagttactc actaatctgt tctaaataat cttgaaaagg ctgtccccaa
      241 aaaggacagc cttttttttc ctgaaatcag tcaatatcct gaacaaaatc agcgttcaaa
      301 ttgtctacta gtatcattaa cgaatcgaaa ggaggtgtgc atgtctaatt atagaatttc
      361 ggcgcacatc ctttgcaccg gtctgtaatg caaaaccggt cgacagacca acaaggtttt
      421 aatcctgccc gtcggcggga ttttttgtta ttaatacctg ctaaaaaaca ggaagcgttg
      481 agtagtttca tctgcaacaa gctaaatctg ctacttttgc cgcaatgaat ttacgcttgt
      541 ccattttgtt tctacttttt tctgctctgc atgcatctgc acagttcggg acaagtaata
      601 cgagcactgg ttccatgaaa tcgggtttcg attcttcagg tgttagtcga gatacttctc
      661 gggttgaact agctggtttg gaactgagcg aaacaggagt taggaaatta caacgcatca
      721 cctattcgct cgatcgattt caattttacc ttcctgttta tcaatcacaa tacattaaca
      781 gcacgatagg aaataacgga acagcaattc agaacctaaa ttttcgtccg acttttaata
      841 gtggatttaa ctggggattt aattcctttt cggcttttac tttagatcta aaggacctac
      901 aattttacga cgcccaatcg ccttataccg aagcagcata cacgcaagga ggaaaacaag
      961 aggcattttt caaaatacgt catatccaaa acgttggtaa atcattcaac tttggtttag
     1021 aattccaacg cgtgaattcg gaaggaacct atttacgcca aaccgcagca cattcagcct
     1081 tgcgtttcaa cttttggtac cgacctggaa aagaacggta tcaaatttta gctggaatca
     1141 actaccataa aggcgcatca ttcgaaaatg gtggaataac tgctgaaggc gattctacct
     1201 ttctaacaaa tacacagtcg aaccgacaac tttatgccgt taatctggta aaagcacgga
     1261 ataaaatttt caaaaacgga ttcttggttc gacaaaccta tgatctagtt caggtaaaaa
     1321 aagattcttc aggaactgtt ctcaatccag ctattctacg cttacaaaac acctttcagt
     1381 atacgttgta tcgacattca tacgacgatg aaaacccgaa taattcatac tattcgacca
     1441 tccttgatac gaacagaact tttgttgatt attacaatcc tcagtgggag aatgaaatag
     1501 cattgttaaa attgagtgct ttagaagact ctgctcaaca aatgcactgg gaagctaagg
     1561 cttttttaaa gcaacaagga gctcatgtgt ggatggaaat tccagtaaat gggatcaata
     1621 gccgaattaa aatcttcaat caaagtgttg gaggaaaaat caattatcat ttccgtccag
     1681 ctttctccgt tcaaggttca accgaattgt tttttgcagg atataatcag ggagatatta
     1741 gtttgtctgg ttccattcat ttaaaaccaa gtaataattg gggttttata gctggagtgg
     1801 aaagttttag tcaagaaccg gtatatcaac ttcggaaatt cgattccaat tttggctctt
     1861 gggataacaa ttcgtttaag aaaattaatc tattacgtgt ttttggaaca atatcacttg
     1921 atcgcattca tacagactta agtgttagca atcaattaat tagcaattgg gttttcatgg
     1981 gcttgaatgc taatcccgaa caagctaatt cagtattaaa tgtccttagg gctgaggttt
     2041 cgaataaaat tcgggtgcaa aaatggtatc tgtatagtcg gatattaatt cagcagacca
     2101 atggaaattc ggtcttgagt ttaccactgg ttcaatatca agagagcatt ttcagagaag
     2161 gaaaaatccg taagacaaca ccttggcgta ttggtatcga tttgattgga tgcacctcct
     2221 ttcaagccaa tgcttatcag cctcagagtg gtttatttta cttgcaaaat caaaagaaga
     2281 actcgggatt gtttcaagct aatttttaca taagtgctaa aattcgtcgt gctcggattt
     2341 ttgctatgct cgaacatgcc aatttcggta ttgcgaataa tcaatcctat gtaattccct
     2401 attatccact acccgaccgc ttgattaaag ttggtttcaa ttgggtattt ttcgattaga
     2461 agcaggaaga tttttcagta ttagcttaca tttgtacaaa ctcgacacat gagatcaatt
     2521 cttactactt tgattatttg cctgactagc ctacaagctt tttctcaggt acagcctggc
     2581 ggtattgctt ttttatcgat gcaatgcgac gctcccgatg gatttgctat tgttactttg
     2641 caagatattc cagctggaac aaccattagt ttcactgata atggatggag cggatcggca
     2701 cttttcacaa acgaaaacac agtaatctgg actgctccgg caacagatgt tccaattgga
     2761 acggttatca tctttcggga agataccatt tctggtgcag gacataacac tgaactcatt
     2821 actggaccag gaacggcaac aggagaatta gctggtttat cgattgatgg agatcagatt
     2881 ctagcttaca ccggaactag cgccaatcca acattcgttg ctgcaataag tacaaatatg
     2941 ttcttaagta cttgcaacat aactggaagt ggtaattcaa attatacctg cctacctgat
     3001 ccactcgaag aa
//
LOCUS       DAOOQW010000269         2981 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_39869_length_2981_cov_5.953862, whole genome shotgun sequence.
ACCESSION   DAOOQW010000269 DAOOQW010000000
VERSION     DAOOQW010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2981
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39869_length_2981_cov_5.953862"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(172..597)
                     /locus_tag="PK281_11135"
     CDS             complement(172..597)
                     /locus_tag="PK281_11135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49942.1"
                     /translation="MNTIKIAILFTVLFFLALTSCNKYEDGPFLSFVSRNERVANTWK
                     MDKAIENGVDVTSRYDRYVVTLTKSGSASLTAHFTLLSLEYDYVTNGTWNFTSNDDKL
                     ELNYENDLVDATYVILKLKEKELWLRQENTELELHLAPE"
     gene            761..1027
                     /locus_tag="PK281_11140"
     CDS             761..1027
                     /locus_tag="PK281_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007650148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HOY49943.1"
                     /translation="MTNNDILKKLRIALHLRNDQIVEILQLVNFRMTASELSAFFRSE
                     DHPNYKNCGDQVLRNFLNGLIIHMRGPMDNKDGSKLEESSDSEN"
     gene            complement(1024..2118)
                     /locus_tag="PK281_11145"
     CDS             complement(1024..2118)
                     /locus_tag="PK281_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOY49944.1"
                     /translation="MIYLFLVLIFFTGVFLFLNTPRFGKTPKGKRLKRIQKLPNYQQG
                     KFQNINLTPDLTQGATYGSVMREILFHKDKRNVPSLPIPAQKIDLFSIPAEEDCIVWF
                     GHSSYYLQIGEKKFLVDPVLNGTASPIAATTRSFKGSDQYTVNDIPNPDYLIITHDHW
                     DHLDYRTVKALQSRTNKVVCPIGVGEHLEHWGYKNAQLIEHNWYESEELEPGFSIHFT
                     PARHFSGRGFKRQSSLWTSYVLKTPNKRLFLGGDSGYDSHFKEIGKQFGPFDLAILEC
                     GQYNKNWEYIHMMPEQVVQAALDLKTEYLFPVHWGKFSLSMHAWDEPIKRVTQAAKDH
                     SLKLMLPQIGEKNPLEGKDYQEFWWESLAD"
     gene            complement(2171..2566)
                     /gene="mutT"
                     /locus_tag="PK281_11150"
     CDS             complement(2171..2566)
                     /gene="mutT"
                     /locus_tag="PK281_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008992559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HOY49945.1"
                     /translation="MIQVTCAILIEEHRILAVRRSMSMNRPGKWEFPGGKIESGETAE
                     QCLIRELQEELAIRVEVIQPLTPTQFNYPDISIQLLPFFVKRVSGEIQLCEHDTFKWV
                     SQTELNQLDWAEADIPIVEEIYSKIDFAS"
     gene            complement(2569..>2981)
                     /locus_tag="PK281_11155"
     CDS             complement(2569..>2981)
                     /locus_tag="PK281_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201843.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HOY49946.1"
                     /translation="ALAGRRPRILVAKMGQDGHDRGAKVISTGFADIGFDVDIGPLFQ
                     TPAEVAKQALENDVHILGVSSLAGGHKTLIPQVIEELKKAGRPDIVVVAGGVIPQQDY
                     NFLFESGVAAVFGPGTSIPLAAQEVLGLLIEGYS"
BASE COUNT          942 a          593 c          530 g          916 t
ORIGIN      
        1 cataaatctc ggtatgactt tgagaaaaac taaaggtggt ttgagcaaat aataaaataa
       61 aaatatagta cttcatgaga tttagctttc atcaaaaata gcaaataaat gctcagaaat
      121 tttcatttcc aagcacttaa ggttgagtaa aaccgaatgg ttaaactact attattcagg
      181 agctaaatgc aattcaagtt ccgtattttc ttgtcgaagc cacagctctt tctctttaag
      241 tttcaaaata acataagtag catcaactaa atcattttca taatttaatt ccaatttatc
      301 atcattactc gtgaaattcc aagtgccatt tgttacatag tcatattcta gacttagcag
      361 agtaaaatga gcagttaagc tagctgatcc cgattttgtt aaagtaacaa catagcgatc
      421 ataacgagat gtcacatcta caccattttc gattgctttg tccattttcc atgtatttgc
      481 tacacgttcg ttacgcgaga caaaacttaa aaaaggacca tcttcatatt tattacaaga
      541 agttaaggca agaaaaaata aaacagtaaa aagaatggca attttaatgg tattcatgtg
      601 gttgatttat tgatttaggt tccatcaaac ctccgaatta tcaacccatt acagttatac
      661 tcttatgtta taaagttgaa taaatcacac atcaataccg aataatcttt cggataatta
      721 gaaatactgc ttgtatattt gcctatctat tggaaaaagc atgacaaata atgacattct
      781 gaaaaagtta cgaattgcac ttcatctaag aaatgatcaa attgtagaaa ttcttcaatt
      841 agtcaatttt cgtatgactg catccgaatt aagtgccttt ttccgtagtg aggatcatcc
      901 gaattataag aattgcggcg atcaagtttt acgtaatttc ttaaacggac ttattatcca
      961 catgcgtggt ccaatggata ataaagacgg ctctaagcta gaagagtcaa gcgattcaga
     1021 gaattagtct gctaaactct cccaccaaaa ttcttggtaa tctttacctt ctagtgggtt
     1081 tttctctccg atttgaggca acattaattt aagagaatgg tctttagcgg cttgtgttac
     1141 gcgctttatc ggttcgtccc aagcatgcat cgacaaacta aatttccccc aatgaaccgg
     1201 aaataaatat tcggttttca agtccaaagc tgcttgaact acctgttctg gcatcatgtg
     1261 aatatactcc cagttcttgt tgtattgtcc acattcgagt atggctaaat cgaatggacc
     1321 aaattgcttt cctatttctt tgaagtgcga atcataaccg ctatcaccac ctaaaaacaa
     1381 gcgtttattg ggtgttttca atacatacga agtccagagc gacgattgac gtttgaatcc
     1441 tcgtccggaa aaatgtcgtg caggagtgaa atgaatgcta aaacccggtt ccagctcctc
     1501 cgattcgtac caattgtgct ctatgagttg ggcattttta tatccccagt gttctagatg
     1561 ctcgccaaca ccaatggggc aaacgacttt atttgttcgg gattgcaagg cttttacagt
     1621 tcggtaatca agatgatccc aatgatcatg cgtaataatt aaataatcag gattggggat
     1681 atcgtttaca gtatactgat cacttccttt gaaactacga gtagtagcag caattgggga
     1741 agcagtgcca tttaaaacgg gatcgaccaa gaacttcttc tctcctattt gcagataata
     1801 ggatgaatgt ccaaaccaaa cgatacaatc ttcttcggct ggtatggaaa agagatcaat
     1861 tttttgagca ggaatcggta aagaaggaac attccttttg tctttatgaa acaagatttc
     1921 ccgcataacc gaaccatacg tggcaccttg agttaaatcg ggtgttagat tgatattttg
     1981 aaattttccc tgctgataat tgggtaattt ctgaattcgt tttaagcgtt tccctttggg
     2041 tgttttacca aaacgaggtg tatttaagaa taaaaaaacg ccagtaaaaa atattaaaac
     2101 aagaaataag taaatcatga tcaagctcat tttgagcgta tgttcaacag tttaaaagct
     2161 gagatgtttt ttaggaagca aaatcgattt tcgaatagat ttcttctaca ataggaatat
     2221 cagcttcggc ccagtcgagt tgatttaatt cggtttgaga aacccatttg aatgtatcat
     2281 gttcgcataa ttgaatttca cctgaaaccc ttttcacgaa aaaaggcagc agttgaatgc
     2341 ttatatcggg atagttgaat tgtgtgggtg ttaatggctg aattacttcc acacgaatcg
     2401 ctaattcttc ctgcaattcg cgaatcaaac attgctctgc cgtttcacct gactcgattt
     2461 taccacctgg aaattcccat tttcccggac gattcatcga catactccta cgaactgcca
     2521 gaatgcgatg ttcttcaatt aaaatggcac aggttacctg aatcatccct acgaataccc
     2581 ctcaatcagc aaacctaaaa cttcctgtgc tgcaagagga atgctggttc caggtccaaa
     2641 cactgccgcc acacctgact caaataagaa attgtaatcc tgttgtggaa ttactcctcc
     2701 agcaacaaca acaatatcag gtcgaccagc tttcttcaat tcttcaatta cttgaggaat
     2761 gagggtttta tgtccgccag ctaatgagga aacacctaaa atatgcacat cattttcgag
     2821 cgcttgttta gccacttcgg ctggagtttg gaatagtggc ccgatatcga catcaaaacc
     2881 aatatccgcg aaacctgtac taataacttt ggcaccacga tcgtgaccat cttgtcccat
     2941 tttagctacc aaaatccgtg gtcttctacc agctaaagcg g
//
LOCUS       DAOOQW010000270         2958 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_40301_length_2958_cov_5.229073, whole genome shotgun sequence.
ACCESSION   DAOOQW010000270 DAOOQW010000000
VERSION     DAOOQW010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2958
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40301_length_2958_cov_5.229073"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /locus_tag="PK281_11160"
     CDS             complement(<1..433)
                     /locus_tag="PK281_11160"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HOY49947.1"
                     /translation="MERAVITGTGAITPLGNSTESFLNNVFAGVSGAGPITRFDASAF
                     KTRFACEVKDFDPTQYFERNESRKYDRFSWYAVAAADEALKMAGISRDNIDVHRAGVI
                     FGSGNGGIETFDQQLIEFAANPTNPRFNPYFIPKMLVNMPSG"
     gene            complement(437..1006)
                     /locus_tag="PK281_11165"
     CDS             complement(437..1006)
                     /locus_tag="PK281_11165"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOY49948.1"
                     /translation="MKIAQERLLELGETLIRTRGFNAISYADLSQKLQVKNAAIHYHY
                     PTKEDLGIAIIRFNREKFAQFCQHMIKREFKPDEQIRRYIRIYSSSLQDAKVCLVGSL
                     GPDFTTFGPAMQNELKNLTSEILDWFTQVLEQGRNEGIFQFKVEPQIQALLIITNLTS
                     ALILSKIMGPTAFHQITDSVMSSLLKTQP"
     gene            complement(1072..1854)
                     /locus_tag="PK281_11170"
     CDS             complement(1072..1854)
                     /locus_tag="PK281_11170"
                     /inference="COORDINATES: protein motif:HMM:NF013575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine synthase A"
                     /protein_id="HOY49949.1"
                     /translation="MRYFFHIGYHGINYRGWQRQPNIIGVQTILEEVLSKVLKENIVV
                     IGCGRTDAQVHASQYFFHIDVKEPWDFDLKFRLNHTLPNDIAVFDILEVGPDQHARFD
                     AIQRDYDYFLHFYKDPFLAKHSSYYRDENLNIPEMQRAAALLPNYSDYFAFCRSPEKY
                     EHTLCFVKSSELISNTKGDRLRFHISSNRYLGSMIRILVGKFLEIGRGEISVDEFESY
                     FHQRKSAPLVIPAHPQGLYLSKVTYPYLDLKPKPIFSFLDFD"
     gene            complement(1867..2520)
                     /locus_tag="PK281_11175"
     CDS             complement(1867..2520)
                     /locus_tag="PK281_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOY49950.1"
                     /translation="MSNSLFIQKTIDYIQVELINAEAGHDWQHIKRVWTNALHIAQFE
                     QVNNLVVELAALLHDIADSKFHHGDETIGPKKAREFLESIHVDENIIQHVVLIIQHIS
                     FKGGNFNREFTSPELQVVQDADRLDAIGAIGIARAFHYGGFKNREIFNPAIQPKLNMN
                     REEYKNDTGPTINHFYEKLLLLKDRMNTQTGKELAEQRHQFMQNFLDQFHAEMEGKR"
     gene            2676..>2958
                     /locus_tag="PK281_11180"
     CDS             2676..>2958
                     /locus_tag="PK281_11180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49951.1"
                     /translation="MEADKINRKKKGQIYLDKKSDLSSEDILLLKMEVLSSYSDESLL
                     LLDKELKVVLFNDRFAELNSKYLKLNVEQGNSIFDYVRPERIDWMKQVYA"
BASE COUNT          893 a          500 c          626 g          939 t
ORIGIN      
        1 cacccgaagg catatttacg agcattttgg gaatgaaata cggattgaat cgagggttgg
       61 taggattcgc cgcgaattcg atgagttgtt gatcgaaggt ttcgatacca ccattaccag
      121 aaccgaaaat cactcctgca cgatgaacgt cgatgttatc tcgagaaata cctgccattt
      181 ttaaggcttc atcagctgca gcgactgcat accaagagaa gcgatcgtat ttacggcttt
      241 cgttgcgttc aaaatactga gttgggtcga aatcttttac ctcacaagca aaacgtgttt
      301 taaaagcaga tgcatcaaag cgggtaattg gaccagcacc tgagactcca gcaaacacat
      361 tattcaaaaa ggactcggta gaattgccta gcggagtgat agcgccagtg ccagtaatga
      421 cagcacgttc catatattag ggttgggttt ttagtaggga agacatgaca gaatcggtaa
      481 tttgatgaaa ggctgtcggt cccattattt tagacaagat gagagctgaa gtaagattgg
      541 taatgataag caatgcttgt atttgcggtt cgactttgaa ttgaaaaatc ccttcatttc
      601 ttccctgttc taggacttga gtaaaccaat cgaggatttc ggaagtaagg tttttaagtt
      661 cattttgcat ggctggtcca aatgtggtaa aatcaggacc gagtgaacca acaagacaca
      721 ctttcgcatc ttgtaagctg gaagagtaaa ttcggatgta tcggcggatt tgttcatcag
      781 gtttgaattc acgtttaatc atgtgttgac agaactgagc gaatttttcc cgattgaatc
      841 gaatgatggc aatgcctaaa tcttctttgg tagggtagtg atagtgaata gctgcatttt
      901 tcacctgcag tttttggctc aaatcggcat agctgattgc gttgaaacct cgggtacgga
      961 tgagtgtttc acctagttca agcaaacgtt cttgtgcaat tttcacggca caaacttact
     1021 tactaataag ttagtttgca aactatattc aaatcttctc gtatctatcg attaatcgaa
     1081 atccaaaaac gaaaaaatag gtttgggttt gagatctaaa tagggataag tgactttcga
     1141 tagataaagt ccttgtggat gagcaggtat gacgagaggt gctgatttgc gttgatgaaa
     1201 atagctttcg aattcatcca cagagatttc accgcgtcca atttccagaa acttcccgac
     1261 gagaattcgg atcatgcttc ctaaatagcg attggaacta atgtggaagc gtagtctgtc
     1321 gcccttggta ttgcttatta gttcggatga tttgacgaaa caaagtgtgt gttcgtattt
     1381 ttcgggagat cggcaaaagg cgaaataatc cgaatagttt ggtaaaagcg ctgctgcacg
     1441 ttgcatttcc ggaatgttta gattttcatc gcgataatag gagctgtgtt tggcgaggaa
     1501 aggatcttta tagaaatgta gaaaatagtc gtaatcacgt tgaatcgcat caaatcgggc
     1561 gtgctgatct ggtccaactt caagaatgtc gaacacagct atgtcattag gtaaagtgtg
     1621 attgagacga aatttaagat caaaatccca gggttctttt acatcaatgt gaaagaaata
     1681 ttgagaagca tgaacctgcg catcggtcct gccacagcct attacaacga tattttcctt
     1741 gagcacttta gaaagaactt cttctaaaat ggtttgaaca ccaatgatat taggttgtct
     1801 ttgccagcct cggtaattga tcccgtgata tcctatatgg aagaaataac gcacggtgta
     1861 aaagtactat cgttttccct ccatttcagc atgaaattga tcaagaaagt tctgcataaa
     1921 ctgatgacgt tgttcggcaa gttctttacc tgtttgtgta ttcattcgat ctttcaacaa
     1981 tagcaatttc tcatagaagt gattgattgt aggtccagtg tcgtttttat attcctcccg
     2041 attcatgttt agtttaggtt gaatagctgg attaaaaatt tctctgtttt taaatccccc
     2101 ataatgaaag gctcgtgcta ttccaatggc gccaatagca tcaagtcggt cggcatcttg
     2161 aacaacttgt agttcgggtg aagtaaattc tcgattaaaa tttcctcctt taaatgaaat
     2221 gtgttgaata attaaaacga catgttggat gatattttca tctacatgga tggattccag
     2281 aaattctctt gctttcttag gcccaattgt ttcgtcgcca tgatggaatt ttgaatcagc
     2341 tatatcatga agaagagcgg ctaattcaac cactaagtta tttacttgct caaactgagc
     2401 tatatgtagg gcattggtcc atactcgttt tatatgttgc caatcgtgtc ctgcttctgc
     2461 atttattaat tcaacttgaa tgtaatcaat ggttttttga atgaatagag aattgctcat
     2521 gatctaagtc attttatttt ataaaatgag tgaaaacacc tatgtaaata tcctcatttt
     2581 acttagggtt taacacatag ttggttacct tggtaaaata tttcataatg gaaatttgat
     2641 atgctaattt caacatagtg aattactgaa ttaatatgga agcagacaag attaatagga
     2701 agaagaaagg acaaatatat cttgataaaa aaagcgattt gtcttcagag gatattcttt
     2761 tattgaaaat ggaagtttta agttcctatt cagatgagag ccttttatta ttagataaag
     2821 aactgaaagt tgttttattt aatgaccgat ttgcagaact taattccaag tatttaaaat
     2881 taaatgttga acaagggaat tctatatttg attatgtaag acccgagcgt attgattgga
     2941 tgaagcaggt ttatgctt
//
LOCUS       DAOOQW010000271         2956 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_40329_length_2956_cov_6.076870, whole genome shotgun sequence.
ACCESSION   DAOOQW010000271 DAOOQW010000000
VERSION     DAOOQW010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2956
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40329_length_2956_cov_6.076870"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1785)
                     /locus_tag="PK281_11185"
     CDS             complement(<1..1785)
                     /locus_tag="PK281_11185"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HOY49952.1"
                     /translation="MALNRHNAPVWIIFLGDLFTCFVSIWAAYLLRFNFRIPADEIVT
                     LPLVFTVVLVVRAVSALVFQTHRGIIRFTNTKDVERIFLSVAAASAIFIAINPVMFWI
                     EKHYLIPYSIIIIDGILSVFLMVIARILIKSILYEFSQERKEKTRVVIYGAGDTGLTA
                     KRAIERDAGAKYKVISYIDDNPQKIGKKVQGISIRSSADLKEILDNYDVRLLIIAIEN
                     LPANTKQSVIELCLANNTKVFNVPAVKSWINGELSFKQIKKININDLLEREPIHLDTH
                     LIQENLSGKTVLVSGAAGSIGSELVKQILPFKPGKLILLDQAESPLYEIERELLDELF
                     VKQIEVVLGDIRSEERMRRVFDHFKPDVVFHAAAYKHVPLMESNPSEAILTNVLGTRN
                     LADLSAEFGVKKFVMVSTDKAVNPTNVMGASKRIAEMYVQSLGQTCSTRFVTTRFGNV
                     LGSNGSVIPIFKKQIERGGPVTVTHPEITRYFMTIPEACQLVLEAGTIGQGGEIFIFD
                     MGKSVKIVDLVRKMIKLYGLTVGKDIEIVYTGLRPGEKLYEELLNDKENTLPTHHPQI
                     MRAKVQEFDHAIVRAQVDDLILMFSNQNN"
     gene            2062..>2956
                     /locus_tag="PK281_11190"
     CDS             2062..>2956
                     /locus_tag="PK281_11190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49953.1"
                     /translation="MHILFIVPYPTGKAASQRFRFEQYYRVLDEKGIAYTIKPFLREE
                     IWNILYLPGRFPRKAMAILGGLFRRFFLLFQLGKYDVIFIHREATPIGPPFFEYFASR
                     WLKKKLVYDFDDAIWIPNYSEANSGFSFLKGYSNVKHICSWASVISCGNQYLCSYARQ
                     YNDKAEIVFNPTTIDTENLHNRVKNQDSERLVIGWTGSHSTIRYLHDLIPVIQKLEKE
                     FEFDFVVISDLPLEVEMKSLVFRKWKKESEIEDLLEFNMGVMPLLDDKWAKGKCGFKA
                     LQYMSLGIPALVSPVGVNTDIV"
BASE COUNT          840 a          604 c          612 g          900 t
ORIGIN      
        1 gttgttttgg ttcgagaaca tcagaattaa atcatccacc tgtgctcgaa caattgcatg
       61 atcgaattcc tgcaccttag ctcgcatgat ttgcggatga tgtgtaggta acgtgttctc
      121 tttatcgttc aacaattcct cgtacaattt ttcacctgga cgtaatcctg tgtacacaat
      181 ctcaatatcc ttcccaaccg ttagtccgta aagcttaatc atcttcctca ctaaatcgac
      241 aatcttaact gatttaccca tgtcgaaaat gaagatttct cccccctgcc ctatcgttcc
      301 agcctcaaga accaactgac aggcttctgg aatagtcatg aaataccggg tgatttcggg
      361 atgcgttacc gttactggtc cgcctcgttc gatttgcttc ttaaaaatgg gaatcaccga
      421 accattcgaa ccgagtacat ttccaaaacg tgtggtgaca aatcgagttg aacaggtttg
      481 tccgagcgat tgcacataca tttctgcaat gcgtttagaa gcgcccatca cattggtagg
      541 attcacagcc ttatcggtcg aaaccatgac aaatttcttc accccaaatt ctgccgacaa
      601 atcagccaaa ttacgagtac ctaaaacgtt cgttagaatg gcttcggaag gattactttc
      661 cataagcgga acatgcttgt atgctgccgc atggaaaact acatcgggtt tgaaatgatc
      721 gaatacccga cgcatacgtt cttcgctacg gatatccccc aaaaccactt cgatttgctt
      781 gacaaacaac tcatctagaa gctcgcgttc aatttcatac agcggacttt cagcctgatc
      841 gagtaaaatg agttttccgg gtttgaaggg taaaatttgt ttcaccaatt cgcttccaat
      901 ggagccagca gcgcccgata caaggaccgt tttaccagaa agattttctt gaataaggtg
      961 agtatcgagg tgaatcggtt cacgttcgag taaatcgttg atgttgattt tcttaatctg
     1021 cttgaaacta agttctccgt tgatccagct tttaactgct ggtacgttga acaccttagt
     1081 attattagct aagcagagtt cgatgacgga ttgttttgta tttgcaggaa gattttcgat
     1141 ggctataatg agcaagcgta catcgtaatt atcgaggatt tccttgagat cggccgaaga
     1201 acgaatgctg attccttgga cctttttccc aattttttgg ggattatcat cgatgtagga
     1261 aattactttg tacttcgcac ccgcatcacg ctcaatggca cgtttggcgg tcaaacctgt
     1321 atcaccagca ccataaatta caacccgagt tttttccttg cgttcttgag agaattcgta
     1381 gagtatcgat ttaatcagga tacgcgcaat aaccatgaga aacacactca aaatcccatc
     1441 gatgatgatg atggaatatg gaatgaggta atgtttctca atccagaaca ttacaggatt
     1501 gatggctatg aaaatggctg aagctgccgc gacagataag aatatccgct ctacatcttt
     1561 ggtattagta aaccgaatga ttccacgatg tgtttgaaac accaatgccg aaacagcacg
     1621 aacaactaaa acaaccgtaa aaacgagtgg tagcgtaaca atctcatcgg caggaatgcg
     1681 gaaattaaaa cgaagcagat aagctgccca tatagaaaca aagcacgtaa ataaatcgcc
     1741 tagaaagatt atccagaccg gtgcattatg tcgattcaat gccatgtgcc aaatataacc
     1801 atttaaaaag aggagaaagt gattggtaaa aggtattaag taattagtat taagtattaa
     1861 gaaatgactg agattagcta tctccctgca accttatact tcatactctc tactttgtac
     1921 tttgttctct ctactttgta ctttgttctc tccactctgt actttccact ctgtacttta
     1981 tactttgtac tatctactct atactttgta ctcatttatg tttcgcgatt ttacttaggt
     2041 ttgcactcct ttcgataaag catgcacata ctttttatcg taccatatcc aacaggcaaa
     2101 gcagcgagtc agcggtttcg ctttgagcaa tattaccggg tgctggatga aaagggaatt
     2161 gcctacacga tcaagccttt tctgagagag gaaatttgga atattctcta tttaccggga
     2221 cgatttcctc ggaaggcaat ggcgattttg ggaggattgt ttcgacgttt cttcttactg
     2281 tttcaattgg gtaaatacga tgtgattttt attcaccgcg aggcaactcc aattgggccg
     2341 ccttttttcg aatatttcgc ttctcgttgg ctgaaaaaga agttggtata cgattttgat
     2401 gatgccattt ggattcctaa ttattccgag gctaacagtg gattttcatt tctgaaaggc
     2461 tattccaatg tgaaacatat ttgctcctgg gctagtgtaa tttcctgtgg gaatcagtat
     2521 ttgtgctcgt atgctcgtca gtataacgat aaggctgaaa tcgtttttaa tccaacaaca
     2581 atcgataccg aaaatctgca caatcgagta aaaaatcaag attcagagcg tttggtaatt
     2641 ggttggacgg gttcccattc gaccattcgt tatttgcacg atttaatacc ggtgattcag
     2701 aaattggaaa aggagtttga attcgatttt gtggtgattt cggatttacc gctggaagtc
     2761 gagatgaaat ctctggtgtt ccgtaaatgg aaaaaagagt cggaaattga agatttgctt
     2821 gagtttaaca tgggtgtgat gccattgttg gatgataaat gggcaaaagg aaaatgtgga
     2881 tttaaggctt tacaatacat gtcgttggga ataccagcct tggtttctcc agtgggcgtg
     2941 aataccgata ttgtgg
//
LOCUS       DAOOQW010000272         2926 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_40886_length_2926_cov_5.694183, whole genome shotgun sequence.
ACCESSION   DAOOQW010000272 DAOOQW010000000
VERSION     DAOOQW010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40886_length_2926_cov_5.694183"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="PK281_11195"
     CDS             <1..194
                     /locus_tag="PK281_11195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49954.1"
                     /translation="LMALFYSLVLEKDIEAVNDMLLGLLALPFGLLSSWLFYRYLSNI
                     WSKEKTSNSIDILDQDLLN"
     gene            252..2024
                     /gene="ggt"
                     /locus_tag="PK281_11200"
     CDS             252..2024
                     /gene="ggt"
                     /locus_tag="PK281_11200"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HOY49955.1"
                     /translation="MKSSHIFICLILLLGHFPSLSAQVFSKQVAGNYSYSDDGKNGKS
                     TVGFYSDKPSVVAENGMVSSAHPLASQIGVDMLKKGGNAVDAAVAVFFALAVVHPSAG
                     NIAGGGFMLFRDVAGNTTSLDFREKAPLAATTDMYLDSSGNIIPNKSTDGIFAVGVPG
                     SVDGMVTAHEKYGNLSWEELLLPAILLAEIGHALTEKEAQGLNQTRADFIQHNPTNSY
                     YRQSKPWKAGDIFIQKDLAASLRRIQTKKRAGFYSGETACLLLQTMQTKQGIISQQDL
                     DDYHSVWRKPIQGKYRNYSVISMAPPSSGGIGLIQLLNMVEPYPLARWGCNSDSSTMV
                     MIEAERRVYADRSTYPGDPDFIRVPVDTLISKTYCARRMKDFSFSHATPSDSILAGYI
                     PNYESDQTTHFSIVDSSGNAVAITTTLNGSFGSKVCVDGAGFLLNNEMDDFSSKAGAP
                     NAYGLTGSLANRIEPGKRMLSSMTPTFVEQDGKLLLVLGTPGGSTIITSVFQTLINVV
                     DYGMTMQQSVNALKFHQQWKPEATRFEKGTFSKQTLRFLKQHGQKMEQLSGTIGRMDC
                     IRVLPDGKLEGGADPRSDNTAIGF"
     gene            2139..>2926
                     /locus_tag="PK281_11205"
     CDS             2139..>2926
                     /locus_tag="PK281_11205"
                     /inference="COORDINATES: protein motif:HMM:NF037626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HOY49956.1"
                     /translation="MFLYVLTSPEILQLYHNHPALKQLLSRIQENAASRLLVKGMAGS
                     QSSFVAAGLFQELAKPLLVIIPDREEAAYFLNDLQCLLPDANVMFFPASYKKPYEPES
                     ADGNNKLLRAEVLNSINSDKRPPIIVSYPEALFERVVTHGNLKKNTFKLKRGERLSIE
                     FVHDFLHEYDFDRVDFVVEPGQFAIRGGILDVFSYSDDEPYRIEFFGEEVDSIRAFNP
                     VDQLSTRNLEFIHIVPDIQGKILRESRESFLEFIPENSLIWIRET"
BASE COUNT          818 a          601 c          599 g          908 t
ORIGIN      
        1 tcttaatggc acttttttac tcactagtgc ttgaaaaaga catagaagcg gttaatgata
       61 tgttattagg cttgttggct ttaccttttg gacttctttc atcttggtta ttttaccgat
      121 atttaagtaa tatttggtcg aaagaaaaga cttctaattc aatcgatatt ttagatcagg
      181 atttattgaa ttaagcatta tcaaatattt catttgagct acatttcctt tttagcgtgt
      241 atcttgttca aatgaaatca agtcacattt tcatttgttt gattcttttg ctggggcatt
      301 ttccctcgtt atcagcacaa gtattttcaa agcaagttgc tggtaattat tcatacagcg
      361 atgatgggaa aaatgggaaa tcaactgttg gattttactc cgataaacct agtgttgtgg
      421 ctgaaaatgg gatggtttcc tcggcacatc ctttagcttc tcaaattgga gttgatatgc
      481 tgaaaaaagg tggaaatgcc gtagatgcag ccgttgccgt gttttttgct ctagccgttg
      541 ttcatccttc tgccggaaat attgctggtg gtggttttat gctttttcgc gatgttgctg
      601 gaaatacaac ttcactcgat tttcgggaga aagcaccctt ggctgctaca accgatatgt
      661 acctcgattc atccggaaat attattccca ataaaagcac ggacggaatt tttgctgtcg
      721 gtgttcccgg ttcggttgat ggaatggtta ctgcacacga gaaatacggc aatctcagct
      781 gggaagaatt gcttttacct gccatattac tagcagaaat cggacatgca ctcaccgaaa
      841 aagaagccca aggtttaaat caaacgcggg ctgattttat tcagcacaat cctacgaatt
      901 cctactaccg acaatcaaaa ccctggaagg ctggagatat tttcattcag aaagatctcg
      961 ctgcttcact tcgacgcatt caaacgaaaa aacgtgcagg attttattcg ggtgaaactg
     1021 cttgcttatt acttcaaacc atgcaaacta agcagggaat tatttctcag caagatttgg
     1081 atgactatca ctcagtttgg agaaaaccca ttcaaggaaa atatcgcaac tattcggtca
     1141 tttcaatggc tccaccttca agcggaggaa ttggattaat tcaacttctc aacatggttg
     1201 aaccatatcc tttagcacgt tggggctgca attccgattc ttctaccatg gttatgatcg
     1261 aagccgaacg tcgtgtgtat gccgatcgtt ctacttatcc cggagatccc gattttattc
     1321 gagttccagt tgatactctc atcagtaaaa cctattgtgc acgaagaatg aaggattttt
     1381 cattttcaca tgcaactcct tcagattcca tacttgctgg ttatattccg aactacgaaa
     1441 gtgatcaaac gacacacttt tcaatcgtcg attcatccgg taatgctgtc gccataacca
     1501 ctacgctcaa tggttcattt ggaagcaaag tgtgtgtgga tggagctgga tttttgttga
     1561 ataacgaaat ggatgatttt agttccaaag cgggggctcc caatgcatac ggactaacag
     1621 gttcactagc caaccgaatc gagcctggga aacgaatgct ttcatccatg acgcccacat
     1681 ttgttgaaca agatggcaaa ttgcttttag tgttaggaac accaggtggt tcaacgatta
     1741 tcacatccgt ttttcaaaca ttaattaatg tggttgatta cggaatgacc atgcaacaaa
     1801 gtgtaaatgc gttaaaattt catcaacaat ggaaacccga agcgactcgt tttgaaaaag
     1861 gcacattttc gaaacaaacg ttgcgctttc tgaaacaaca cggacaaaaa atggaacagc
     1921 tttctgggac aattggcaga atggattgta ttcgtgtact acctgatggt aaattagaag
     1981 gaggagctga tcctcgttcc gacaatacag ctattggatt ttaaggtcag attaaatttt
     2041 taactgtcgg atatttcttc tttaagggct gaagctttcc ttatattcgc gctgctaaac
     2101 ccgcagaaca ttcttccccg agaaccgttc tgctttcttt gtttctgtac gttttgactt
     2161 cacccgaaat acttcagctt tatcacaatc atccggcact taaacaactg ctgagtcgca
     2221 ttcaagaaaa tgccgcaagc cgtttactag tgaagggaat ggcaggttcg caatcttcat
     2281 ttgtggctgc tggattattt caggaattag ctaaaccgct tttagttatc atccccgatc
     2341 gggaagaagc ggcatatttt ctcaacgatt tgcaatgctt gttgccggat gcgaatgtga
     2401 tgttctttcc agcttcctat aaaaagccat acgaacccga aagtgctgat gggaataaca
     2461 aattgctacg tgctgaagtc ctaaatagca tcaattccga taaacgtcct cccattattg
     2521 tttcctatcc cgaagcgctt ttcgaacgtg tggtgacgca tggaaacttg aagaaaaaca
     2581 ccttcaaact caagcgtggc gaacgtttat ccatcgagtt tgtgcatgac tttttgcatg
     2641 aatacgattt tgatcgggtt gattttgtgg ttgaacccgg acaattcgcc attcgtggag
     2701 gaattctaga tgtgttttct tattcggatg atgagcctta ccgaatcgag ttttttggtg
     2761 aagaagtcga ctccattcgt gcttttaatc cagtagatca gttatctacc cgaaacctcg
     2821 aattcattca catcgttccc gatattcaag gtaaaatact tcgagaaagt cgcgagagtt
     2881 ttctggaatt catccccgaa aattcactca tctggattcg agaaac
//
LOCUS       DAOOQW010000273         2908 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_41201_length_2908_cov_5.221171, whole genome shotgun sequence.
ACCESSION   DAOOQW010000273 DAOOQW010000000
VERSION     DAOOQW010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41201_length_2908_cov_5.221171"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="PK281_11210"
     CDS             complement(<1..360)
                     /locus_tag="PK281_11210"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOY49957.1"
                     /translation="MLKRFWSATKYYFFLAIGLLLLYIVFKDVDFRWMWNEIKHAHFG
                     WIFISFICGFLAMASRAWRWRIMLEPLGYKPGFWNCFNGVSIGYLSNIALPRMGEVIR
                     CTILNQTNGIPVTKLFGT"
     gene            complement(367..717)
                     /locus_tag="PK281_11215"
     CDS             complement(367..717)
                     /locus_tag="PK281_11215"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016479237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HOY49958.1"
                     /translation="MHIQVLKSKIHRVTVTEADLNYIGSVTIDQDLMDAANLIENEKV
                     QVLNMHNGERLETYVIKGERGSGVICMNGPAARKVSVGDIIIIISYAMMDFEAAKSFS
                     PWVIFPDTDTNKLK"
     gene            complement(764..1609)
                     /gene="panC"
                     /locus_tag="PK281_11220"
     CDS             complement(764..1609)
                     /gene="panC"
                     /locus_tag="PK281_11220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HOY49959.1"
                     /translation="MILFRAIPELRQYIQDIRNSGKTLGFVPTMGALHAGHLSLIEKS
                     RLENDITLLSIFVNPTQFNESSDFTNYPRTEEKDLDLIRSLQIEAVFLPTTEEMYRSG
                     VKDLLDFPLDGLDRVMEGKYREGHFEGVVTIVDKFFALIEPDKAYFGLKDFQQLAIIR
                     YLAQKRYPSIQIVPCEIVRESDGLAMSSRNVLLQPEERKKAVLISQVLFSIQDLWKSR
                     PIAEIIDLAKQKFEHSSLDLEYLEIADSANLQSLEDYSGKPAVACIAARIGKVRLIDN
                     CELPA"
     gene            1857..2690
                     /locus_tag="PK281_11225"
     CDS             1857..2690
                     /locus_tag="PK281_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011711384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HOY49960.1"
                     /translation="MTKTRVLFVSQEITPYLPETHLSKVARELPQAIQERGREIRTFM
                     PRYGCINERRNQLHEVIRLSGMNLIVDDNDQPLIIKVASIQAARMQVYFIDNEEYFQR
                     KFIVTDKNDQFYADNDERMLFFCKGVIETVKKLGWSPDVIHCQGWMAALLPILIKRNF
                     KDDPLFAESRMVVSLLDDSFSGNMDHRLAEKLIMDGIDKEDVTLLKEPSYLNLMRHAI
                     SFADGVIVAQENASEDLIQFAAQLKKPLLGLQTDETYADAYSAFYDEVLLGEPVAEMA
                     E"
     gene            2713..>2908
                     /locus_tag="PK281_11230"
     CDS             2713..>2908
                     /locus_tag="PK281_11230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49961.1"
                     /translation="MKKFRLTYPEHSFWSVPGFLLFSCLFIGFNACKQPDLGLEVQNP
                     SDAINLQKKDDFTVRTTLERE"
BASE COUNT          869 a          603 c          579 g          857 t
ORIGIN      
        1 ggtgccgaat aatttggtga ctggaattcc attcgtttga ttgagaatag tacagcgaat
       61 tacctcaccc atacgcggaa gggcgatatt tgaaagatat cctatcgata caccattgaa
      121 gcagttccag aaaccaggtt tgtaaccaag cggttcaagc atgattctcc aacgccaagc
      181 acgtgatgcc atagccaaga aaccacaaat gaaggaaatg aaaatccatc caaaatgagc
      241 gtgttttatt tcattccaca tccagcggaa atccacgtct ttaaaaacaa tgtagagcaa
      301 cagtagcccg atcgccagaa agaaataata tttagtggcc gaccagaagc gcttcaacat
      361 gatagtttat ttcagtttgt tggtgtcggt gtcggggaaa ataacccaag gagagaagga
      421 tttagcggct tcaaaatcca tcatagcata ggaaataatg atgatgatat ctccaacact
      481 cactttgcgg gcagcaggac cattcatgca aataacacca gaacctcgct cacctttaat
      541 cacgtatgtt tcgagacgtt ctccattatg catgttcaag acctgcactt tttcattttc
      601 aatcagatta gcggcatcca tcaaatcttg atcgatagta acgcttccga tataattcag
      661 atcagcttcg gtaacagtga cccggtgaat tttcgatttt aaaacctgaa tatgcataaa
      721 cgggcgcaaa cctacggcaa atacctcgaa tccacaatgc gactcaggca ggtaattcac
      781 aattatcaat cagccgaacc tttccaattc tcgctgcaat gcaggcaaca gctggtttcc
      841 ctgaataatc ttcgagcgat tgtagattgg cagaatcggc tatctcaaga tattctaagt
      901 ccaaacttga atgctcaaac ttttgtttag ccaagtcgat tatttcagct attggtcgtg
      961 atttccacaa atcctgaata gaaaacaaaa cttgagaaat gagaactgct ttcttgcgtt
     1021 cctccggttg taaaagtaca ttccgcgaac tcatcgctaa accatctgat tctctaacaa
     1081 tttcgcaagg aactatttga atacttggat atcgtttctg agctaagtat cggataatag
     1141 ctaattgttg aaaatccttc aatccaaaat aagctttatc aggctctatc aaagcgaaga
     1201 atttatctac aatcgttacc actccttcga aatgaccttc tcggtacttc ccttccatca
     1261 ccctatctaa accatccaaa gggaagtcaa gtagatcttt aacgcccgaa cgatacattt
     1321 cttcggtagt aggaagaaaa acggcttcga tttgaagcga acgaattaaa tctaggtctt
     1381 tttcctctgt acgaggataa tttgtgaagt cggacgactc attaaactga gttggattga
     1441 caaaaatgct cagcaatgta atgtcatttt cgaggcggga tttctcaatt aaagagagat
     1501 gtccggcatg aagagcgccc atagtaggca caaagccaag ggttttaccc gaattacgaa
     1561 tatcttgaat gtattgccta agctcgggaa tggctcgaaa tagaatcatc tagaaaaaag
     1621 aaaatgcatg cctcgatcaa atggctaatc tccgcttaat ccgtcaatta catggtcaga
     1681 ggctgcaaag ctaacggatt gaccttgatt ttaaccaaat ttttttcgta attttgtaat
     1741 ctgatttacc aaatcaccta acatggatta tttgtgccgt agttttttta gttccggctt
     1801 tccttattgg aatgaatcca tgaaatcttc ctccttattc tcttaattga cttgctatga
     1861 cgaagacaag agtattgttt gtttcacagg agattacccc gtatttgccg gaaacgcatc
     1921 tttctaaagt agctcgtgag ttgcctcagg ccattcagga gcgcggacgt gagattcgta
     1981 ccttcatgcc ccgttacggt tgtatcaacg aacgccggaa ccaattacat gaagtaatcc
     2041 gcttatccgg aatgaatctt attgtggacg acaatgatca gccattaatt attaaagtgg
     2101 cttctattca ggctgcgcgg atgcaggtct atttcatcga taacgaagag tattttcaac
     2161 gtaaattcat tgtaaccgac aagaatgatc agttttatgc cgacaatgat gagcgcatgt
     2221 tgtttttctg caaaggtgtt atcgagacgg ttaaaaaact aggttggtct cctgatgtaa
     2281 tccattgtca aggttggatg gcagcattgc ttccaatttt aatcaaacgt aatttcaaag
     2341 acgatccgtt gtttgctgaa agtcgtatgg ttgtttctct tttagatgat tcattctcag
     2401 gaaatatgga tcatcgttta gctgagaaac ttattatgga tggcattgat aaagaagatg
     2461 ttactctatt gaaagagccg agctatttga atttaatgcg tcatgcaata tcttttgctg
     2521 atggcgttat tgtagcgcaa gaaaatgcat ctgaagactt aattcaattt gctgctcaat
     2581 taaagaaacc attacttggc cttcaaacag atgaaaccta tgcagatgca tattctgcat
     2641 tttatgacga ggttctcttg ggggagccgg tcgccgaaat ggctgagtaa ttaccgattt
     2701 ttacagtatc ccttgaaaaa attccgcctt acatacccgg aacattcctt ttggagtgtt
     2761 ccgggtttcc tgttgtttag ctgccttttt attggcttca atgcgtgtaa acaacctgac
     2821 cttggtctag aagttcaaaa cccttccgat gcaattaatc ttcagaagaa agatgacttc
     2881 acggttcgta ccactttgga gcgggaag
//
LOCUS       DAOOQW010000274         2901 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_41365_length_2901_cov_4.154252, whole genome shotgun sequence.
ACCESSION   DAOOQW010000274 DAOOQW010000000
VERSION     DAOOQW010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41365_length_2901_cov_4.154252"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="PK281_11235"
     CDS             complement(<1..276)
                     /locus_tag="PK281_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HOY49962.1"
                     /translation="MDQSQQNHLKSYGIAYWILQHELELHWLVNYRGGSFLVGNSKAV
                     ENECIIRGVSYQMIADAQANAILLEIAAPEVNMDAVKLEKAPKVAVYS"
     gene            complement(630..2204)
                     /gene="dnaB"
                     /locus_tag="PK281_11240"
     CDS             complement(630..2204)
                     /gene="dnaB"
                     /locus_tag="PK281_11240"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HOY49963.1"
                     /translation="MKENTPQTNSDRKKRIYTGVNVNPYDAGIGKLPPQAVDVEEAVL
                     GALMLDRDSLANVIDSLKPETFYRDEHQRIFGAIFELFASSKPVDMLTVVQELKKRGE
                     LDMVGGVPYITSLTNRVASTANIEYHTRILNQKQIQRELIRVSSDIIKDAYEESTDAL
                     ELLDKAEKNLFAVAEGNIKKNFSKMSELITEAKKQIEKAGQQQDGLSGIPSGFLALDR
                     FTAGWQKSDLVILAARPGMGKTAFVLSLARNAAVDYKRPIAVFSLEMSSVQLVMRLIS
                     GEAELPGEKLKKGDLQQHEWHQLDSKIRELTEAPLFIDDTPALSIFELRAKARRLKAN
                     HDIQMIIIDYLQLMTAGGDNRGGGNREQEISQISRSLKGIAKELEIPVIALSQLSRNV
                     EQRGGTKVPQLSDLRESGAIEQDADMVMFIYRPEYYNITENEDGSPTNGLAEIHIAKH
                     RNGALGVVPLKYIGHLAKFTDMEGTHSLNPNMRPSGLMPNVNFDSSVQETITVGSKMN
                     SASGNKDNWDPDNVPF"
     gene            complement(2339..>2901)
                     /locus_tag="PK281_11245"
     CDS             complement(2339..>2901)
                     /locus_tag="PK281_11245"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HOY49964.1"
                     /translation="FARILGKLAQLTDSGIAIHLFTGNHDMWAFGYLEKELGLIIHRK
                     PEVTELQGKKLFLGHGDGLGPGDYGYKFIKKVFASKVCQWLFARLHPNFGIGLADFWS
                     RKSRAANPEKEEFLGEDNEWLLIFSKEQLQRQPNLDYFIFGHRHLPLDLLVGQECRYV
                     NLGDWFHWCSYAELRDGNLSLKYFNE"
BASE COUNT          859 a          638 c          483 g          921 t
ORIGIN      
        1 agagtaaaca gctactttgg gtgctttttc gagcttaacc gcatccatat tgacttccgg
       61 agcagctatt tccaataaaa ttgcatttgc ctgtgcatca gcaatcattt gataactaac
      121 acctcttata atgcattcgt tttcaaccgc tttagaattt cctacaagaa aactaccacc
      181 acgataatta actagccaat gcaattccaa ttcatgctgt agaatccaat aagcaatgcc
      241 gtatgatttt aaatgattct gctgactttg atccatagga attaggatcg acgccgattt
      301 caagctttgg ccccctaaac atagaaccaa caaaagcaac aatacgattc gtttcataag
      361 taagcaacgt gaattgttga acggtatttt ttctaaagtg ttctaaaacg aatgagaatc
      421 tgtgaatttt tgggcgtacc cgtataaaag tttcgctatg ctacacttta atccggtcgc
      481 gttttacgct tcaagtcctc cttcgtattc gattcactta aattttaaac ttaaatttta
      541 gcttcgacca atatacaagc attttcactc agtccgggct atccgcttca accgcttacg
      601 caacaataca actaaaacta gctagctact taaaatggaa cattatcagg atcccaattg
      661 tctttattac ctgaagctga gttcatttta gaacctactg ttatcgtttc ctgaaccgag
      721 ctgtcaaagt ttacattagg cattaaaccc gaaggacgca tattcggatt tagcgaatgt
      781 gttccttcca tatcagtaaa cttggcgaga tgtccgatgt atttaagcgg cacaactcct
      841 aaagctccat ttctatgctt tgcaatgtga atttcagcta agccatttgt tggactacca
      901 tcttcattct cagtaatatt atagtattcc ggacgataga tgaacatgac catatcagca
      961 tcctgctcaa tagctcccga ttcacgtaaa tcgctcaatt gaggcacttt ggttcctcct
     1021 cgttgctcca cattacgact taactgagat agggcaataa caggtatctc taattccttt
     1081 gcaatacctt taagagacct tgagatttga gagatttctt gttctcggtt tccaccgcct
     1141 cgattatctc cgccagctgt catcaattgc aaataatcga tgatgatcat ttgaatatca
     1201 tggttggctt ttaatcttct ggcttttgca cgtaactcaa aaatggatag ggcaggggtg
     1261 tcgtcaataa ataaaggagc ttcggttagt tctctaattt tggaatccaa ctgatgccat
     1321 tcatgttgct gtaaatctcc ttttttcagt ttctcaccag gtaattcagc ttctccagaa
     1381 atcaaacgca taaccaactg aacactcgac atctccagag agaacactgc aattggacgt
     1441 ttataatcga cagctgcatt tcgtgcaaga gacaaaacga atgctgtctt acccatacct
     1501 ggacgagcag ccaaaataac caaatcggat ttctgccaac ctgctgtaaa acgatctaaa
     1561 gccaagaaac ctgaaggaat accacttaat ccatcttgtt gttgtcctgc cttttcgatt
     1621 tgtttttttg cttctgtaat gagctcactc attttagaga agttcttttt gatgttacct
     1681 tctgcaacag caaataagtt cttctctgct ttatctaaca attcaagagc atcagtgctt
     1741 tcttcgtatg cgtctttaat aatatcactc gaaactcgaa ttaattcacg ctgtatctgt
     1801 ttctgattta agatacgggt atgatattca atgtttgctg ttgaggcaac tcggttggtt
     1861 aaggaggtaa tataaggtac accgccaacc atgtccaact cgccacgttt ttttagctct
     1921 tgaacgactg ttaacatatc aacaggtttc gaagaggcaa acaattcgaa aatcgccccg
     1981 aaaattcgtt gatgttcatc ccgataaaac gtctcgggtt ttaacgaatc aatcacattt
     2041 gctagagaat cacgatcaag catcaaggct cctaaaacag cttcttccac atcaactgct
     2101 tgaggtggta acttacctat tccagcatca tatggattaa cattgacacc ggtataaatc
     2161 cgtttttttc tatcagaatt tgtttgtgga gtgttctctt tcatcggatg acaaatatac
     2221 cattcatcaa cctcttaacg gagattgttt ataaatcatt attgcatttt tcagaatggt
     2281 tgaattttaa atttaatgtt gaaattcaac tgcttatgta tgtgcttttg tacttgtctt
     2341 attcgttgaa gtacttcaga ctcaaattac catctctcaa ttctgcatag gaacaccaat
     2401 ggaaccaatc acccaaatta acataccgac attcttgacc aaccaaaaga tcaagtggta
     2461 aatgccgatg accaaatatg aaataatcca aatttggttg tcgttgcaac tgttctttgg
     2521 agaatatcag taaccattca ttatcttctc caagaaattc ttctttctcg ggattagctg
     2581 cacgactttt ccttgaccaa aaatcggcca gtccaattcc aaaatttgga tgcaaccgag
     2641 caaaaagcca ttgacaaact ttgctagcga aaaccttctt tatgaatttg tatccataat
     2701 ctccagggcc aagtccatca ccatgtccga gaaaaagctt tttaccttgt aattcagtta
     2761 cttcaggttt tcgatgtatg atgagcccaa gttctttttc taagtaacca aaagcccaca
     2821 tatcgtgatt cccggtaaat aaatgaatcg caattccaga atcagtcaat tgagctaact
     2881 ttcctaaaat tcgggcgaaa t
//
LOCUS       DAOOQW010000275         2810 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_43219_length_2810_cov_5.253358, whole genome shotgun sequence.
ACCESSION   DAOOQW010000275 DAOOQW010000000
VERSION     DAOOQW010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2810
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43219_length_2810_cov_5.253358"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..921)
                     /locus_tag="PK281_11250"
     CDS             complement(<1..921)
                     /locus_tag="PK281_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017842962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOY49965.1"
                     /translation="MEIPTKYTPASTEDKWYKRWMDSGCFRSTPDNREPFTIVIPPPN
                     VTGVLHMGHMLNNTIQDVLIRRARMQGKNACWVPGTDHASIATEAKVVSKLRAEGIEK
                     NSLTREEFLSHAWEWKEKHGGIILEQLKKLGASCDWERTKFTMDPPLSEAVIDVFIDL
                     YKKGLVYRGVRMINWDPSAQTALSDEEVNYKETPSKLYYVKYQTEDGSDSVTIATTRP
                     ETILGDTAVCVHPEDERYSQLVGKNIMVPLINRAIPVIADEYVDREFGTGALKVTPAH
                     DVNDYMLGIKYNLPVIDVIASDGSMSPAATL"
     gene            1160..1591
                     /locus_tag="PK281_11255"
     CDS             1160..1591
                     /locus_tag="PK281_11255"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HOY49966.1"
                     /translation="MKKIFAMLLFSGSLTLAAQPATTDLNWTPTATFTKTEHDFGKIP
                     EGPKVNTEFEFKNTGKEPIIVTNAAAGCGCTTPEFEKTPIAPGKTGKIKVGFNSEGRP
                     GNFTKDVTVTFTSGVNSDKTGTTKITIKGSVTPKAAEPAKH"
     gene            1698..2171
                     /locus_tag="PK281_11260"
     CDS             1698..2171
                     /locus_tag="PK281_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014033424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HOY49967.1"
                     /translation="MKRSLLSMAFLAMGFVALHAQTPQPASTEQNWTPAIEFEKETHD
                     YGTIKNGADGTCEFKFKNTGKEPFVISNARGSCGCTVPDWPKEPIGPGKSGVVKVSYD
                     TKRSGAINKNVTLTIQNMAKDKTGTKMIYIKGNVEPPPADAGMPEKKSGMAPLEK"
     gene            2233..>2810
                     /locus_tag="PK281_11265"
     CDS             2233..>2810
                     /locus_tag="PK281_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOY49968.1"
                     /translation="MPSISAKGKAMPSSPIRKLVPFAEAAKKRGVKIYHLNIGQPDIE
                     TPEVALNAIRNTDLKVIEYSHSAGFESYRRKLAAYYKQNHIDVDHEQILITTGGSEAL
                     QMAIMTCMDPGDEIIIPEPFYANYLSFACSAGAKVVPVKTSIDTGFKLPPIEEFESLI
                     TPRTKAIIISNPGNPTGMIYSREDLEKLRDII"
BASE COUNT          873 a          553 c          564 g          820 t
ORIGIN      
        1 taaggttgca gcaggactca tacttccatc actcgcaatc acatcgatta caggtaggtt
       61 gtatttaatc ccaagcatat agtcgtttac atcatgcgca ggagttactt taagcgctcc
      121 tgtaccaaat tcacgatcaa catattcatc cgcaatcacc ggaatagccc gattgataag
      181 tggtaccatg atgttcttgc ctacgagttg gctataacgt tcgtcttctg gatgaacaca
      241 cactgcagta tcacctaaaa tcgtttcagg acgagttgtg gcaatagtta cagaatcaga
      301 accatcttct gtttgatact taacgtagta tagtttggat ggcgtttcct tgtagtttac
      361 ttcttcatcc gaaagggcag tttgagcaga aggatcccaa ttgatcatgc gcacaccacg
      421 gtaaaccagt ccttttttat aaaggtcgat gaatacatct ataactgctt cgctcaaagg
      481 cggatccatg gtgaatttgg tgcgttccca atcgcatgaa gcgcctagtt tctttagctg
      541 ttctaagata attcctccgt gtttttcttt ccattcccaa gcatggctga ggaattcctc
      601 acgtgtaagg ctgttttttt caatgccttc agcgcggagt ttacttacca cttttgcttc
      661 agttgcaatt gaggcatggt ctgtaccagg aacccagcaa gcattttttc cttgcatgcg
      721 agcacgacga atcaatacat cttgaatggt attgttcaac atgtgtccca tgtgaagcac
      781 tccagtaaca ttgggtggag gaatgacaat cgtaaatggc tcacgattat ctggagtaga
      841 acggaagcaa ccgctatcca tccagcgttt gtaccatttg tcttcggtgc ttgcaggggt
      901 atatttcgta gggatttcca tagtatttct tggggacgca aaaataggtg aatttgccac
      961 gctgagaaac aggaaactaa agaggtgttt tttatggatt tcatctttta attgatacag
     1021 ttgtatgttt taatctagag aaatgattgt ttaagcattg aaaattagtc agttgggtat
     1081 ttttagccgt gtaattaact tattgatttt tggcttggta attgctaatg ccgccccgaa
     1141 tttccaacga cttatcatta tgaaaaaaat cttcgcaatg ctgctatttt caggcagcct
     1201 gactttggca gctcaacctg caacaactga tttaaactgg acaccaacag cgacatttac
     1261 taaaacggaa catgatttcg gtaaaattcc tgaaggtccc aaagtgaata cagaatttga
     1321 atttaagaat accggaaaag aaccaattat cgtaaccaat gcagcagctg gttgcggatg
     1381 tacaactcca gaatttgaaa aaacgccaat tgcaccgggt aaaactggaa aaataaaagt
     1441 tggttttaat tctgaaggcc gtccaggaaa ctttactaag gatgttaccg taacatttac
     1501 tagtggtgta aacagtgata aaactggaac aactaagatt actattaaag gttcagttac
     1561 acctaaggca gccgaacctg caaaacacta ataatttaat tctgagaatc ggatactttg
     1621 tttaacattg ttgacgctgc atcagaagca aaaagaattt taaatttgtc atcacttaat
     1681 ctcaaaacaa caccaaaatg aaacgatccc tgttatcaat ggcgtttctg gcaatgggct
     1741 tcgttgctct tcatgcccag acgccacaac cggcatccac tgaacaaaat tggacgccag
     1801 ccattgaatt tgaaaaagaa acacatgatt atggtacaat taaaaatggt gctgatggaa
     1861 catgtgaatt caaattcaag aacaccggaa aagagccgtt tgtaatttct aatgcacgtg
     1921 gtagctgtgg ttgtacagtt ccagattggc caaaagaacc aatcggacca ggtaaatcag
     1981 gtgttgtgaa agttagttat gacacgaaac gttctggtgc cattaacaaa aatgttactt
     2041 tgactatcca aaacatggcg aaagataaaa ctggaaccaa aatgatttac atcaaaggta
     2101 acgttgaacc gccaccagca gatgcaggaa tgcctgaaaa gaaaagtgga atggctccac
     2161 ttgaaaaata aggaatcctc cccgaaaatt ttcatctttg caccccgact caatgtcggg
     2221 gttttttgtt ttatgccaag tatatcagct aaaggaaagg caatgccttc ctctcccatc
     2281 cggaaactcg ttccttttgc agaagctgca aagaaaagag gtgttaaaat ttatcatttg
     2341 aatatcggac aaccagatat tgaaacacct gaagtagctt taaatgccat tcgtaataca
     2401 gatcttaaag taattgaata cagtcatagt gctggatttg aaagttatcg tagaaagtta
     2461 gcagcttact acaaacaaaa ccacattgat gttgaccatg agcagatact cataacaact
     2521 ggtggttcgg aagcacttca aatggcaatc atgacttgta tggatccagg agatgagatt
     2581 attattcctg aaccatttta tgctaattat ttgagttttg cttgttctgc tggggctaaa
     2641 gtcgttcctg ttaaaactag tattgataca ggttttaagc tgccccctat tgaggaattt
     2701 gaatcgttaa taacacctcg aacaaaagcg attatcattt caaatccagg taatcctact
     2761 ggaatgattt attctcgtga ggacttagaa aagcttcgtg atataatctt
//
LOCUS       DAOOQW010000276         2790 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_43648_length_2790_cov_4.074954, whole genome shotgun sequence.
ACCESSION   DAOOQW010000276 DAOOQW010000000
VERSION     DAOOQW010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2790
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43648_length_2790_cov_4.074954"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2790
                     /locus_tag="PK281_11270"
     CDS             <1..>2790
                     /locus_tag="PK281_11270"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOY49969.1"
                     /translation="SSYKAYRNGKFSTELVYVDELFDQFSYGITQHPLAIRNFANTAL
                     AYWSTKPQYMLLLGKSISAHFIRTDPQAWSECLVPTYGFPSSDILLTAGLNGALPYEP
                     ALATGRIAAKNGADVTAYLNKLQEYEGNEPALWMKQILHFGGGQSQGEQQTYLNYLST
                     YKNTIEDTLFGGTVYTYQKTSSAPIEQTQSAIIADYINNGVSLMTFFGHATGSGFDVS
                     IDDPASYSNQGKYPFLIGNSCYAGDIHQPPGFGFSQSEEFTLIPNRGTIGFIASVSLG
                     IPNQLHTYTDSLYKYIGQSHYGDPVGLNMKRVCHEIYSEDPFRKAVVLETTLHGDPGL
                     AMNHADYPDYQTSAPDVYFNPTDVTTEVDSFQIKIVVTNVGRAVSQPVTVEVVRNFPG
                     SGLTTTYSRVMPNLFYRDTLSITMPVTDLVKGGVGINTFNIRVDPLNQQTELSEVNNN
                     ITATLIIRSAEVLPVWPYEFALVPNDTITLKASTGDPFAPVRTYIFELDTTDLFNSPF
                     KITNSITQAGGVLSWSHPITLQDSMVYFWRVSPDSIAGGSYRWKESSFQYIPGKRGWS
                     QDHFFQYKRNTFNQINYLRPQRYFDFVTNAKEISCYAYGYPWEISELWATSYKIDAEV
                     WADAGCSLDGAVYIAVIDSVTLLPWLSPQNCWDIGNQFGQFNNTCQCKGGNRMANFVF
                     RNEGSASEMNAMRDMINSVPNGHYILAYTWIYGNYQTWDESLYQAFEDLGADSVRYLP
                     NEYPWIFLAKKGHPESAIELIADSANANLVLSAPMYNSWVSGDFTSTLIGPGSGWDTL
                     SWKAKNFDNPLDSIRIQVKGIQANGTEDIILEATIGSTGDVNLAPLIGTTAYSYLKLN
                     AFMRDDSLRTPVQLDRWQVTFDPVPECALNPLADLYLNNDTIQQGDSLRLAISIQNIS
                     ELPMDSMLVAYWIQDA"
BASE COUNT          801 a          579 c          571 g          839 t
ORIGIN      
        1 tctagttaca aagcataccg gaacggaaaa tttagcaccg aactggttta tgtagatgag
       61 ctttttgatc aattttcata tggaataacc caacacccgc tcgccattcg aaatttcgct
      121 aatacagcac ttgcctattg gagcaccaag cctcaataca tgttgctttt aggaaaatct
      181 atctctgctc atttcattcg aaccgatcct caagcttggt ccgaatgctt agttcctacc
      241 tacggttttc cttctagtga tatactttta accgcgggat tgaatggtgc gcttccctat
      301 gaaccggctc ttgcaactgg acgaattgcc gctaaaaacg gagctgatgt tacggcatac
      361 ctcaataaac ttcaggaata cgaaggaaat gaacccgcgc tttggatgaa acaaattctc
      421 catttcggtg gaggacaaag tcagggtgaa caacaaacgt atctgaatta cttatctact
      481 tataagaata cgattgaaga taccttgttc ggaggaacag tttataccta tcaaaaaacg
      541 agttccgctc caattgaaca aacccaaagt gccatcattg ccgattacat caataatggt
      601 gtttcgctca tgaccttctt tggacatgct accggaagtg gctttgatgt gagtattgac
      661 gatcctgctt catacagtaa tcaaggtaaa tatccattcc taattggaaa ctcatgttat
      721 gcaggtgata ttcaccaacc tcctggattt ggtttcagtc aaagtgagga atttacactc
      781 atcccgaatc ggggaactat tggtttcatt gcttctgttt cgctcggaat tcccaatcaa
      841 cttcatactt acaccgattc actctacaaa tacataggac aaagccatta tggcgatccg
      901 gttggtttga atatgaaacg ggtttgtcat gaaatatatt ccgaagatcc tttccgtaaa
      961 gctgttgtac tcgaaactac attacacggc gatcccggct tagccatgaa ccacgccgac
     1021 tatcctgatt atcaaaccag tgccccggat gtgtatttca atccaactga tgtaacaacc
     1081 gaagtggatt cattccaaat taaaatagtc gttacaaatg tgggaagggc ggtaagtcag
     1141 cctgttactg tcgaagtagt tcgaaatttc cccgggtctg gtttaacaac tacctattcg
     1201 cgtgttatgc cgaatttgtt ttaccgcgat acactttcta tcaccatgcc ggttactgat
     1261 ctagtaaaag gaggtgtggg aatcaacaca ttcaatattc gggttgaccc acttaatcaa
     1321 caaaccgagc tttctgaggt aaataataac atcacagcaa ctttaattat tcgctctgcc
     1381 gaggtattac cagtttggcc gtatgaattt gccttagttc caaacgatac catcaccctc
     1441 aaagcatcta ccggagatcc ttttgctcct gtaagaacct atattttcga actcgatact
     1501 accgacttat ttaatagtcc gtttaaaatc accaattcca taacacaagc gggtggagta
     1561 ctgagttggt cgcatccaat tacactgcaa gatagcatgg tctatttttg gcgcgtatct
     1621 cccgattcaa ttgcaggtgg aagttatcgc tggaaagaaa gttcgtttca atatatccct
     1681 ggaaaaagag gctggtcaca ggatcatttc tttcaatata agaggaacac cttcaatcag
     1741 attaattact taaggcctca acgttacttc gactttgtaa caaacgcgaa ggaaatttct
     1801 tgctatgcct atggctatcc ttgggaaatt agtgagttgt gggctacttc ctataaaatt
     1861 gacgctgaag tttgggcgga tgcgggttgt agtttagatg gtgcggtata tatagctgta
     1921 atcgattctg taactctgtt gccttggctt tccccacaaa actgttggga tattggaaat
     1981 cagtttggac agtttaataa tacctgtcaa tgtaaaggag gaaatcgtat ggccaatttc
     2041 gttttccgta acgaaggctc tgcatcggag atgaatgcca tgcgagatat gataaatagt
     2101 gttccgaatg gacattatat tctagcctat acttggattt atgggaacta tcaaacttgg
     2161 gatgaaagct tgtatcaggc ttttgaagat ctaggagctg atagtgttcg atatttacca
     2221 aacgaatacc cttggatttt cttagctaaa aaaggtcatc ccgaatctgc aatcgaatta
     2281 atcgccgatt ctgctaatgc taatttggtt ttaagtgctc ccatgtataa cagttgggta
     2341 agtggcgatt tcacttctac cttaattggt ccaggttctg gatgggatac cttaagctgg
     2401 aaagccaaga atttcgataa tccgctagat agtattcgta ttcaggtgaa aggaattcag
     2461 gcaaatggaa ctgaagatat catcttagaa gctactatag gctcaacggg tgatgtaaat
     2521 cttgctccac ttattggcac cactgcttat agttatttga agttaaatgc ctttatgcgg
     2581 gatgattcac tgagaactcc agtacagtta gatcgctggc aagtaacttt tgaccctgta
     2641 ccagaatgtg cattgaatcc attagctgat ttgtacttga acaacgatac cattcaacag
     2701 ggcgatagtc ttcgcttagc tatttctatt caaaacatta gcgaattgcc gatggatagc
     2761 atgttggttg cttattggat tcaagatgcg
//
LOCUS       DAOOQW010000277         2764 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_44222_length_2764_cov_5.060908, whole genome shotgun sequence.
ACCESSION   DAOOQW010000277 DAOOQW010000000
VERSION     DAOOQW010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44222_length_2764_cov_5.060908"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /locus_tag="PK281_11275"
     CDS             complement(<1..620)
                     /locus_tag="PK281_11275"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HOY49970.1"
                     /translation="MLQSSIRLLTTKKSAFEQFRLGWIAFLLTILTNLHAQSPFGFIY
                     NSSIQAYDLNDSNPLSNAWTGGFNAPQFNECELNGDNLLDLIVFDRTGNKLLCFTRNS
                     DGNSFRFAPEFESVFPKLHDWMSLRDYDKDGKPDIFASGGNGIEIYRNTSPSTTTTQF
                     SKVVDLVYSDYGPSSLNLYVSPVDFPAIDDVDGDGDLDIVTFYILGT"
     gene            complement(586..1116)
                     /locus_tag="PK281_11280"
     CDS             complement(586..1116)
                     /locus_tag="PK281_11280"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010135851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HOY49971.1"
                     /translation="MTIQDKVKATIRDVADFPKPGILFKDITPILENPLLCREITVEL
                     ARAYENISIDAIVGVESRGFLFGMLLAQQFNVPFIPVRKAGKLPYETIQYSYNLEYGS
                     ATVEMHVDALKPEWNVLVHDDLLATGGTAMAAAELIQQQRAHVAGFSFLVELDFLNGR
                     DALQKYAPIVDSLINY"
     gene            complement(1113..>1409)
                     /locus_tag="PK281_11285"
     CDS             complement(1113..>1409)
                     /locus_tag="PK281_11285"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOY49972.1"
                     /translation="GGFYSITQLSEIKGFSDSLLTCVKIQVEIDSSRIQKIKINHISL
                     EEIQKHPYCWYGVGKSIVNYRLQHGPFASVEDLKKIYTLRPEIYAKLVHYIIIE"
     assembly_gap    1410..1509
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1627..2535)
                     /locus_tag="PK281_11290"
     CDS             complement(1627..2535)
                     /locus_tag="PK281_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOY49973.1"
                     /translation="MKRILITGSNGLLGQKLVYKSVGRPGVELIATAKGENRLILREG
                     YTYVPMDIGNKADVDSVIDQYRPDVVINTAAMTNVDACETDKEGCVLLNMTAVEYLVE
                     ACNRNKAHFIHLSTDFIFDGENGPYDEEAEANPVSYYGWSKAEAERIVKERSNDWTIL
                     RTILVFGLVDHMSRSNVVLWAKGALEKGAPINVVDDQFRMPTLAEDLAEGCLLAAEKH
                     ANGIFNISGKDYMSVLELVHQVADFYGYDKSIINTVSSSTLNQPAKRPPRTGFDLSKS
                     ERVLGYHPHSFREGLAILDEQLKKYQ"
BASE COUNT          781 a          541 c          581 g          761 t
ORIGIN      
        1 gttccgagga tgtaaaatgt gacaatatcc aaatcaccat caccatccac atcatcgatt
       61 gcagggaaat ctaccggact aacataaagg ttcagagaac ttggcccata gtctgaataa
      121 accaaatcta ccaccttaga aaattgagtg gtagtagtgc taggcgaagt gttacgatag
      181 atttcaattc catttccacc ggaagcgaaa atatcgggtt tgccgtcctt atcgtaatca
      241 cgaaggctca tccagtcgtg aagtttagga aatacggact caaattcggg agcgaatcgg
      301 aacgagtttc cgtcactatt acgagtaaag caaagcagtt tatttccggt acgatcgaaa
      361 acgattaggt cgagcagatt gtcgccattg agttcacatt cgttgaattg tggtgcatta
      421 aaaccgcccg tccatgcatt ggataaagga ttggaatcgt ttaggtcgta tgcttgaatg
      481 ctggagttat aaataaatcc aaagggcgat tgtgcatgca gatttgtcaa gatggttagc
      541 aaaaacgcaa tccatcccaa gcgaaactgc tcgaaagctg atttcttagt agttaataag
      601 cgaatcgacg attggagcat atttctggag tgcatctcga ccattaagga agtcgagttc
      661 aactaagaaa gagaatccag ctacatgagc tcgttgttgt tgaatgagtt cggctgcagc
      721 catggcagtt ccgcctgtag caagcaagtc gtcatgtaca agaacattcc attcgggttt
      781 gagcgcatcc acatgcattt ctactgtagc agaaccgtac tctaagttat aggaatattg
      841 tattgtttcg tatggtagtt ttcctgcttt acggacagga ataaaaggca cgttaaattg
      901 ttgtgcgagt agcattccaa aaaggaaacc gcgactttct acacctacaa ttgcgtcaat
      961 ggaaatgttc tcataagctc gagccaattc taccgtaatt tctctgcata aaagcggatt
     1021 ttcgagaata ggggtgatgt ctttgaagag aattcctggt ttaggaaaat ccgccacatc
     1081 gcggatggta gcttttactt tgtcctgaat ggtcattcaa ttataatgta atggaccagt
     1141 tttgcataga tttcgggacg aagggtatag attttcttta agtcttcgac cgaagcaaat
     1201 ggtccgtgtt ggaggcggta atttacaatg cttttgccca cgccatacca acaataaggg
     1261 tgtttttgaa tttcttcgag tgaaatatga ttgattttga ttttttgaat gcgggaagag
     1321 tcgatttcta cttgaatctt cacgcaggta aggagcgaat cgctgaagcc ttttatttct
     1381 gacagttgcg tgatggaata aaatccaccn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnc gggagaaatg caggaattct cgaatcaatg ttttcatgtc ggttgtttgg
     1561 ataaacttcc gaaactgaaa tcgatggatt cgtctgtgaa acgtttatat acgtttaaaa
     1621 cgattattac tggtacttct tcagctgctc atccaaaata gcaagtcctt cccggaagct
     1681 gtgaggatga tatcctaaaa cacgttccga cttagataaa tcgaatccag tacgtggcgg
     1741 acgtttagcc ggttgattta aagtgctaga tgaaacggtg ttgatgatgc ttttgtcgta
     1801 gccataaaaa tcggccactt gatgcaccaa ttcaagtacg ctcatatagt ctttgcccga
     1861 tatattgaaa atgccgttag catgtttctc tgctgctagc aaacaacctt cagctaaatc
     1921 ttcagccagt gttggcatgc gaaactgatc atcaaccaca ttgattggtg ctcctttttc
     1981 gagagcacct tttgcccaaa gaaccacatt cgaacggctc atgtgatcga ctaatccaaa
     2041 aactaaaatg gtacgtaaaa ttgtccaatc gtttgaacgt tcttttacaa ttcgctccgc
     2101 ttcggctttc gaccagccat aataagatac cggattggct tctgcttcct catcgtacgg
     2161 accattctcc ccatcaaaaa tgaaatcggt tgaaaggtga atgaaatgcg ccttgttccg
     2221 attgcaggct tctaccaaat attcaacagc agtcatattc agcaggacgc atccttcttt
     2281 atcggtttca caggcatcca cattcgtcat ggcagctgtg ttaatcacca catccggacg
     2341 atattgatca atcacgctat ctacatcggc tttgttacca atgtccatag gaacataggt
     2401 gtatccttcg cgcagaatga gtcggttttc acctttcgca gtggctatca attcaacccc
     2461 tgggcgacca acacttttgt aaacgagttt ttgtccgagt aatccgttgc ttccggtaat
     2521 gaggatacgt ttcatgccct atgagtggat agattttggg gtgaaaatac cgaaaaggaa
     2581 actaggagta taaagtaaaa agtaataaag tacaaagtag ttgagtataa agtagaaaag
     2641 tataaagtag aaaagtataa agtagaaaag taaaaagtag gaaagtatta agactgtttt
     2701 aaactcgggc attcgttgac ctcacccccg gcccacttgc cactatgtgg tctccgtacc
     2761 gaag
//
LOCUS       DAOOQW010000278         2747 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_44603_length_2747_cov_3.785661, whole genome shotgun sequence.
ACCESSION   DAOOQW010000278 DAOOQW010000000
VERSION     DAOOQW010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44603_length_2747_cov_3.785661"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..723)
                     /gene="clpX"
                     /locus_tag="PK281_11295"
     CDS             complement(<1..723)
                     /gene="clpX"
                     /locus_tag="PK281_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013305245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HOY49974.1"
                     /translation="MERIKEKCSFCGRSKKETAILIAGLDAFICDVCVTQAYQIVTEE
                     LAERKGGTVKQELNLLKPLEIKLHLDEYVIGQDEAKKVLSVAVYNHYKRLSQKNFKID
                     EDTEIEKSNIILVGETGTGKTLLARTISRILNVPFCIADATVLTEAGYVGEDVESILA
                     RLLQAADYNVEAAQRGIVFIDEVDKIARKSDNPSITRDVSGEGVQQGLLKLLEGAVVN
                     VPPQGGRKHPEQKLIPVDTKNIL"
     gene            complement(730..1404)
                     /gene="clpP"
                     /locus_tag="PK281_11300"
     CDS             complement(730..1404)
                     /gene="clpP"
                     /locus_tag="PK281_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004569159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HOY49975.1"
                     /translation="MFHNDEFRKYAVKHRGISSSSLDSYTSITSDYISPTIIEERQLN
                     VAQMDVFSRLMMDRIIFLGVGINDYVANIVQAQLLFLESVDAKKDIQIYMNTPGGSVY
                     AGLGIYDTMQIIAPDVATICTGMAASMGAVLLCAGAEGKRTALKHARVMIHQPMGGAQ
                     GQASDIEITAREIQKLKKELYDIIAGHSGQTYDKVEKDSDRDYWMTAEEAKAYGMIDE
                     ILARKK"
     gene            1629..2747
                     /gene="ahcY"
                     /locus_tag="PK281_11305"
                     /pseudo
     CDS             1629..2747
                     /gene="ahcY"
                     /locus_tag="PK281_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013597859.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
BASE COUNT          784 a          579 c          556 g          828 t
ORIGIN      
        1 aagaatattt tttgtatcca ccgggatgag tttttgttcc ggatgtttac gtcccccttg
       61 aggaggaaca ttcacaactg caccttccaa tagctttaac aatccttgtt gtacaccttc
      121 accagaaacg tcacgtgtga tagaaggatt atcactttta cgggcaattt tatctacctc
      181 gtcgataaat acaattcctc gctgagcagc ttctacatta taatcggcag cctgaagcaa
      241 gcgggcaaga atgctttcca catcctcgcc cacatatcct gcttcggtta aaacggtagc
      301 atcagcaata cagaatggta cattgagtat gcgtgaaata gtccgcgcaa gcaatgtttt
      361 tcctgtacct gtttctccta ccaaaatgat attcgacttt tcgatttcgg tatcctcgtc
      421 gatcttgaaa ttcttctgag acaaacgttt gtaatggttg taaaccgcaa ccgaaagcac
      481 ttttttagct tcatcctgac caataacata ttcgtcgaga tgcaacttta tttcaagagg
      541 tttaagaaga ttgagttctt gcttaacagt tccacctttc cgttcagcta gttcttccgt
      601 cactatctga taggcttgtg ttacacacac gtcacagata aatgcatcca aaccagcaat
      661 cagaatagcg gtttcctttt tactgcgtcc acaaaacgaa catttctcct taatcctttc
      721 cattctatct tactttttac gcgctagaat ctcgtcaatc ataccgtaag ctttagcttc
      781 ttcggctgtc atccaataat cgcgatcgct gtctttttct actttgtcgt aggtttgtcc
      841 tgaatgtccg gcaataatgt cgtacaattc ttttttcaac ttctgaattt cacgagcagt
      901 gatttcgata tcagaagctt gcccttgagc tccacccatt ggttggtgaa tcatcacacg
      961 agcgtgtttc aaagcagtac gttttccttc ggcaccagca caaagtaata cagctcccat
     1021 agaagctgcc attccggtac aaattgttgc aacatcagga gcgataatct gcatggtatc
     1081 gtaaataccc aatccagcat ataccgaacc tcccggagta ttcatatata tctgaatatc
     1141 ttttttagca tcaacacttt ctaagaaaag caattgagct tgtacaatgt tggctacgta
     1201 atcgttaatt cccacaccta aaaagataat acgatccatc atcaaacgcg aaaacacatc
     1261 catttgtgcc acgttaagct gacgctcttc gatgatagta ggggagatgt agtcggaagt
     1321 gattgatgta tagctgtcca gcgaactgga tgaaatgccg cgatgcttaa ccgcgtattt
     1381 tctgaactcg tcgttatgaa acataggaga attgaatttg taaaggaacg aaaattttcc
     1441 ggcgggtttt cagcaattat gccagtgccg gaatactgac aatatggcga tgcttagagg
     1501 gcacctaatt tccggacaag tttctcaaag tgtttgggct tctgagcgca tccttcagaa
     1561 agatttcaac acaaaaccgg ttaattgcct gaagatgtat tacctttgcg cccgaaatcg
     1621 ataaactcat ggaaaataac gtagctgtgg aaaactacat tccttttaaa gtaaaagaca
     1681 tctcactagc tgagtggggt cgtaaagaaa tccgtttagc ggaagcagaa atgccaggat
     1741 tgatggcact tcgcgaagaa tttggaacaa ctaaaccgct tcaaggagct cgtgttgctg
     1801 gatgtcttca catgacaatt caaacagccg ttttgattga aacattggtt gttttaggtg
     1861 ctgaagttac ttggtcttct tgtaatattt tcagtactca ggatcatgct gctgctgcaa
     1921 ttgctgctgc gggtattcct gtttatgctt ggaaaggcat gactgctgaa gaatttgact
     1981 ggtgtatcga acaaactctt ttcttcggtc cagatcgtaa accattaaac atgattttgg
     2041 atgatggtgg agatttgacc aatttggtat ttgataaata tcctgaatta gctcaaggaa
     2101 tccgtggtct ttctgaagaa acgactaggt gttcaccgtt tatatgagcg tatgaaaaaa
     2161 ggaactctac ttgttcctgc tatcaacatc aacgattcag ttaccaaatc taaattcgat
     2221 aacaaatacg gttgccgtga gtctttagtt gacgcaattc gtcgtgctac tgatgtcatg
     2281 atggctggaa aagtagcagt tgttgctggt tacggtgatg ttggaaaagg ttctgctgat
     2341 tccttgaaaa actcaggtgt acgtgttatc gttactgaga ttgaccctat ctgtgcactt
     2401 caagctgcaa tggaaggtta cgaagtgaaa aaaatggaaa cggctatcaa agaagctgat
     2461 atcgtggtta ctgcaacagg taacatgaat atcgttactg agcgtcactt ccgtgcattg
     2521 aaagacaaag caatcgtttg taacattggt cacttcgata cagaaatcga tatggcttgg
     2581 ttaaacggag catacggaca aacaaaagac accatcaaac cacaagttga cttatacaac
     2641 atcgatggca aagaaattat cgttcttgct gaaggtcgtt tggtgaattt aggatgtgct
     2701 actggtcacc cttcatttgt aatgtctaat tcattcacca atcaagt
//
LOCUS       DAOOQW010000279         2735 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_44868_length_2735_cov_6.607463, whole genome shotgun sequence.
ACCESSION   DAOOQW010000279 DAOOQW010000000
VERSION     DAOOQW010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2735
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44868_length_2735_cov_6.607463"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            23..1414
                     /locus_tag="PK281_11310"
     CDS             23..1414
                     /locus_tag="PK281_11310"
                     /inference="COORDINATES: protein motif:HMM:NF020390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HOY49976.1"
                     /translation="MTEAQKTAHLLRRAGFGYSLRDKSNLSFDEALTRLFSSNNTYEE
                     LYLQGEERVTMAELRKMTKEERREILDDLAANVKKLNLDWLKIMGKADGQLREKMSLF
                     WHGHFAVRVRGFAQVESYINTLRKYALGNFGDLLMAMSKEPAMLRFLNNVQNKKNSPN
                     ENFAREVMELFTLGRGNYTEHDIKDAARAFTGWGVNDSDEFELKVNQHDTGSKTIFGK
                     TGNWEGEDVIRMILERDETSIYITRKIYRFFVNDQVNETHVAALAKDFKASNYDIPKL
                     LKQLFTADWFYADENQGAHIKSPVELIVELNRTFGISYDNAQPLLAVQRVLGQTLFFP
                     PNVSGWAGGRNWIDNSTLITRMNLPKKLAEAGGLDMSFKSMDDESPNEFQQASQLKNK
                     FNCRFNWELFEAKFADVATENLWQAVSENLLASKIKPDLPDYLKQKLTNPRPEIIRLM
                     ALYLVRLPEYQLA"
     gene            1418..2608
                     /locus_tag="PK281_11315"
     CDS             1418..2608
                     /locus_tag="PK281_11315"
                     /inference="COORDINATES: protein motif:HMM:NF019040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HOY49977.1"
                     /translation="MKRRQFLQLSGLTTTAALIPGFLKAFETNLSALSGKRIVIVQLS
                     GGNDGLNTLVPFSNNIYYEARPRLAIAADQVLKISDDAGFHPALLGMKYLYDEGMMSI
                     YNSVGYPNPDRSHFRSMDIWHTASNSDEYLNTGWIGRLLDSNCKGTEHAWFAIEADDT
                     LSLAMKGSRRSGFAVKDPKRLHAALSDPFYQQVQVGKQASEQESELSFLYKTLASTTS
                     GADYIYEKSQAKPSSASYPQHELGKSLKEISGLIQAGSESRIYYTSISGFDTHVRQAE
                     TQQRLFQVVGDSLKSFCMDLKASGNFNDTVVMVFSEFGRRVKQNASNGTDHGTANSVF
                     LLGGNLKNAGLRNAMPDLTKLDDGDLIHQLDFRRIYASLLDQGIGFDSTQILGRKFDS
                     LDFI"
BASE COUNT          820 a          469 c          647 g          799 t
ORIGIN      
        1 ttgaatagca aattctcatt ttatgacaga agcacagaaa actgcccatt tacttcgaag
       61 agccggtttt ggttattcct taagagataa aagcaatttg agctttgatg aagcattaac
      121 tcgtttattc tcaagcaata acacttacga agagttgtat ttgcaaggtg aagaacgagt
      181 tacgatggct gagcttcgga aaatgacgaa ggaagaacgc agagaaatcc tagacgattt
      241 agctgcaaac gtgaagaaat taaatctcga ttggcttaaa atcatgggga aagcagatgg
      301 acagcttcga gaaaaaatgt cgctgttctg gcatggtcat tttgctgttc gcgttcgcgg
      361 attcgctcag gttgaatcgt atatcaacac tttacgtaaa tatgctttgg ggaattttgg
      421 tgatttgttg atggctatgt cgaaagagcc agcgatgttg cgtttcttga ataatgtgca
      481 gaataagaag aatagtccga atgagaattt tgctcgcgaa gtgatggaac ttttcactct
      541 tggacgagga aattataccg aacacgatat aaaagatgca gctcgagcat ttactggatg
      601 gggcgtgaac gattcggatg agtttgaatt gaaggtcaac cagcacgata cgggtagtaa
      661 aacaattttc gggaaaacgg gaaattggga aggagaagat gtgattcgga tgattttgga
      721 acgagatgaa acctcaattt atatcacccg aaaaatctat cgcttttttg tcaatgatca
      781 ggtgaatgaa acgcatgtag cggcattagc caaggatttc aaagcatcga actacgacat
      841 tccgaaatta ctcaaacaac ttttcacggc tgattggttt tatgccgatg aaaaccaagg
      901 tgcgcatatc aaatcgccgg tagaattgat tgttgagcta aaccgaacgt ttggaatttc
      961 atacgacaat gctcagcctt tgctagctgt tcagcgagtt ttaggacaaa ctttgttttt
     1021 ccctcccaat gtttcgggtt gggctggtgg aagaaattgg atagataatt caactctcat
     1081 tactcgaatg aatcttccca aaaaattagc agaagcgggt ggtttggaca tgagttttaa
     1141 aagcatggac gatgaaagtc cgaacgaatt tcaacaggct tcacagttga aaaacaagtt
     1201 taattgtcgg tttaattggg aattattcga agccaaattt gccgatgtag ctaccgaaaa
     1261 cctatggcaa gctgtgtcgg agaatttatt agcaagcaag atcaaacctg atttaccgga
     1321 ttatttaaaa caaaagttaa cgaatccgcg accggagatt attcgcttaa tggctcttta
     1381 cttagttcgt ttacctgaat accaactcgc ttgaattatg aaacgtcgtc aatttcttca
     1441 actaagtggt ttaacaacta ctgccgcttt gattccaggt tttctaaaag cctttgaaac
     1501 gaatttgagt gctttaagtg ggaaacgtat tgtgattgtt cagctttcgg gcgggaatga
     1561 tggtttgaat acgctcgttc cgtttagcaa caatatctat tatgaagctc gtccgcgttt
     1621 ggcgattgca gccgatcagg tattaaagat tagtgatgat gcgggcttcc accctgccct
     1681 attgggaatg aaatacttgt acgacgaggg aatgatgagc atttacaatt cggttggata
     1741 tccgaatcca gatcgttcgc attttcgatc tatggatatt tggcacaccg ccagtaattc
     1801 ggacgaatac ctcaacacgg gttggattgg acgtttgctc gattcgaact gcaaaggcac
     1861 agagcatgct tggttcgcta ttgaagcaga tgatacgttg tcgttggcta tgaaaggaag
     1921 tcggcgaagc ggttttgcgg tgaaagatcc caagcgttta catgcagctt tatcggatcc
     1981 attttatcag caagtacagg ttgggaaaca agcttcggaa caagaaagtg agttatcgtt
     2041 tttatataaa acccttgctt caaccacttc tggtgcagat tacatttatg aaaaatcgca
     2101 agcaaaacct tcgagcgctt cgtatccgca acacgaatta ggtaaaagct tgaaagagat
     2161 ttcgggattg atacaagcag gaagtgaatc gcgtatttac tatacgagta tttcgggttt
     2221 cgatacacat gtaaggcagg cagagacaca gcaacgcttg tttcaggtgg tgggagattc
     2281 gctcaaaagt ttctgtatgg atttgaaagc atcggggaat tttaatgata cggtggtgat
     2341 ggtattttcg gaattcgggc gacgagtgaa acagaacgct agtaatggaa cagaccatgg
     2401 aacggcgaat tcggtatttc tgttaggtgg aaatttgaaa aatgccggat tgcgaaatgc
     2461 gatgcccgat ttaacgaaat tggatgatgg agatttgatt catcagcttg attttaggcg
     2521 catttatgcc agtttgttgg atcaaggaat tggctttgat tcgactcaaa tattaggaag
     2581 aaaatttgac agtttagatt ttatttaacg cattgtgcgg ttgaaactac ggttttatat
     2641 tttcatttca agcaatgaaa taaagctgtt aaagcatctt tcaataaatc attcatgttt
     2701 gtctacgacg tgttactttc ttgtcttgaa acaag
//
LOCUS       DAOOQW010000280         2727 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_45057_length_2727_cov_5.094311, whole genome shotgun sequence.
ACCESSION   DAOOQW010000280 DAOOQW010000000
VERSION     DAOOQW010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2727
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45057_length_2727_cov_5.094311"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /locus_tag="PK281_11320"
     CDS             <1..523
                     /locus_tag="PK281_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOY49978.1"
                     /translation="ILFICGGAFDGIDKKIARRIQSQTIGYATNKDTEVIDKENLLKY
                     INPQDLKSFGLIPELIGRLPVLSHLDPLDKDALKRILTEPKNALIKQYKKLFSIDHVD
                     VDFEDNVLDFIVEKANEFKLGARGLRSICEGIMTDLMFELPSDPSIKKYTVTLKYAKA
                     KFDKLKLNHLKAA"
     gene            complement(527..1117)
                     /locus_tag="PK281_11325"
     CDS             complement(527..1117)
                     /locus_tag="PK281_11325"
                     /inference="COORDINATES: protein motif:HMM:NF025224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HOY49979.1"
                     /translation="MSKLGILILFLFTFTLGVNAQPDYTTEGKVFTSLKEASLQPDSV
                     FRLKLRRKNYKEIPPEILKFKNLRELDLISNNIKRIPSDIGKLEKLEILRLERNQIDV
                     VGKEIGNLPNLKYLDLGKNRIQALPYEIGNLSSLEFLQIWGNEITLIPESIQKLEKLK
                     YLDMRAILLTESEKEDVLEQLPNTEVLLSPGCNCGK"
     gene            complement(1114..2046)
                     /locus_tag="PK281_11330"
     CDS             complement(1114..2046)
                     /locus_tag="PK281_11330"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HOY49980.1"
                     /translation="MQVHYSLESFPKLSFPVVTTGTFDGVHLGHQTILNRLQELARKY
                     NGQSVVITFHPHPRQVLRPDDHSLQLLTTLPEKIARLEVSGIDHLLVIPFNKEFAELS
                     SQDFIRQVIVEAVNTRKLVIGYDHHFGKNREGSFEHLRQFGPQYGFEVEEIPAQDIDQ
                     VAVSSTKIREALSRGDIHTANRYLGYAYPFNGKVVRGKQLGRTIGFPTANLELEGLGK
                     LIPANGVYAVMVNSGSGIFNGMMNIGFRPTVSDEKVRSIEVHLLDFEGDLYEQNMKVK
                     LIARIRDEKRFASVDELRDQLKLDRVQALKYLSV"
     gene            2273..>2727
                     /locus_tag="PK281_11335"
     CDS             2273..>2727
                     /locus_tag="PK281_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015481896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HOY49981.1"
                     /translation="MALSTGKIVQVIGPVIDVSFDTQSNKLPNILDALEVTRADGQKL
                     IMECQQHIGEDTVRAISMDATDGLSRGMVVTATGQPITMPIGDQVKGRLFNVVGEAID
                     GIGATDSKGGYSIHREPPRFEDLSTSKEILFTGIKVIDLIEPYSKGGKIG"
BASE COUNT          829 a          545 c          555 g          798 t
ORIGIN      
        1 catcctattt atatgtggtg gtgctttcga tggaattgat aagaaaattg cccgtcgtat
       61 tcagagtcag accattggtt atgcgaccaa taaagacaca gaggttatcg acaaagaaaa
      121 tcttcttaaa tacatcaacc cacaagattt aaaaagcttt ggattaattc ctgagcttat
      181 tgggcgttta cctgtactta gtcatttaga tccactcgat aaagatgccc taaaacgtat
      241 tcttaccgaa ccaaaaaacg cattgattaa acagtacaaa aagctgttta gcattgatca
      301 tgtggacgtt gacttcgaag ataacgtatt agattttatt gtagagaaag ctaacgaatt
      361 caaattagga gcacgtggtt tacgatctat ttgtgaaggt attatgaccg atttgatgtt
      421 cgaattacct tctgatccta gcattaagaa gtacacggta acacttaaat atgctaaggc
      481 taagttcgat aagttgaaac ttaaccattt gaaagccgct taaacgctac tttccacaat
      541 tacaaccggg tgataaaagc acttctgtat taggaagttg ttcgagcacg tcttcttttt
      601 ccgattcggt gaggagaatg gcgcgcatgt cgagatattt tagtttctct aacttttgaa
      661 tgctctccgg gatgagcgtt atttcattcc cccatatttg aagaaactca agagaactca
      721 aattgccaat ttcgtagggc aatgcttgaa ttcggttctt tcctaaatcg aggtatttga
      781 gattgggcag attgccgatt tctttaccaa ctacatcaat ttgatttcgt tccaaccgaa
      841 gaatttcgag tttttcgagt ttccctatat cacttggaat acgtttaatg ttgttagaaa
      901 tgagatccag ttcgcgcagg tttttaaatt tcagaatttc gggagggatt tctttgtagt
      961 tcttacggcg cagcttcaac ctaaaaacag aatcgggttg gagactcgct tctttcaaac
     1021 tggtaaatac ttttccttcg gtggtatagt cgggttgagc atttacacca agagtaaatg
     1081 tgaacagaaa taagataaga atacccagtt tactcatacc gaaagatact tcagggcttg
     1141 cacccgatct aattttagtt gatcacgtag ctcgtccaca ctagcgaaac gtttttcatc
     1201 ccgaattcgt gcaatgagtt tcactttcat gttttgttcg tacaaatctc cttcaaaatc
     1261 gagcaaatga acttcaatac tacgaacctt ttcatccgaa acagttggcc ggaagccaat
     1321 attcatcatt ccattaaaaa taccacttcc cgaattaacc atcacagcat aaacgccatt
     1381 ggccggaata agtttcccta atccttcgag ttcaagattt gccgttggaa atccaattgt
     1441 tcgaccgagt tgtttacctc gaacaacctt tccattgaaa ggatacgcat aacctaaata
     1501 acgattggca gtatgaatat cgccccgcga aagtgcttct ctaattttgg tagagctaac
     1561 ggctacttga tcgatgtctt gagctggaat ttcctctact tcaaaaccat attgaggtcc
     1621 aaattggcgc aaatgttcaa atgaaccttc tcggtttttc ccaaagtggt gatcataacc
     1681 aataactagc ttccgagtat taacagcttc tacaattacc tggcggatga aatcctgcga
     1741 gcttaattca gcaaactctt tattaaaagg aattaccagc aaatgatcta tccccgaaac
     1801 ctctaaacga gctatctttt cgggaagtgt ggttaaaagc tggagtgaat gatcatcggg
     1861 acgaagaacc tgacgcggat gcggatgaaa ggtgataaca accgactgtc cgttgtattt
     1921 gcgggctaat tcctgaagtc ggtttaagat agtttgatgc ccgagatgaa caccatcaaa
     1981 ggttccagta gtaactacgg gaaaagaaag tttaggaaaa gactcgagag aataatgtac
     2041 ctgcacggat gctatgttgc tgacaaacct aaatgaaaaa tggcgagaat acaaccgcat
     2101 cggggaatgg gaatgaacaa gaggttaata acacaaaaaa ttgatactgt gtcgttttgc
     2161 acattccatc atttttcttg ttcatttttt acaaaatact gttttacgcc aaaaatttcg
     2221 attaatttcg cgccggattt cagggttacc tgattcaatt aaaaaactcc ttatggccct
     2281 aagtactggc aaaatcgtac aggtcatcgg tccggtgatc gatgttagct ttgatacgca
     2341 aagcaacaaa ctacctaata tcctagatgc acttgaagta actcgtgccg atggtcagaa
     2401 gctgattatg gaatgtcagc agcacattgg tgaggacaca gttcgtgcaa tctccatgga
     2461 tgcaaccgac ggtttaagcc gcggaatggt ggtaacagca acaggacagc ctatcactat
     2521 gccgattggc gatcaagtga aaggtcgttt gtttaacgta gtaggtgaag ctatcgacgg
     2581 aattggagct accgacagta aaggtggtta ttcgattcac cgtgagccac cacgtttcga
     2641 ggatctttct acttcaaaag aaattctctt cacaggtatt aaagtaatcg acttgatcga
     2701 gccttattca aaaggtggta aaatcgg
//
LOCUS       DAOOQW010000281         2678 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_46143_length_2678_cov_4.595501, whole genome shotgun sequence.
ACCESSION   DAOOQW010000281 DAOOQW010000000
VERSION     DAOOQW010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46143_length_2678_cov_4.595501"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..534)
                     /gene="rimM"
                     /locus_tag="PK281_11340"
     CDS             complement(4..534)
                     /gene="rimM"
                     /locus_tag="PK281_11340"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HOY49982.1"
                     /translation="MKQEQCFQLGVFTRKVGTDGRLSLQLDTDQPEAYTKTESVFVEL
                     HGKLVPFFVLSFRLQPSNSAHIKLEDVDNAEQADSLTGCLVFLPLEKLPPLSGTKFYY
                     HEIIGWKIEDLTSGLSAGIIKDVLENGPNDLFQLTNEGQEVLIPVAEDWIVKVDRELG
                     VISMNLPEGLLEVNRK"
     gene            complement(549..1100)
                     /locus_tag="PK281_11345"
     CDS             complement(549..1100)
                     /locus_tag="PK281_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOY49983.1"
                     /translation="MPVKIRLQRHGKKSYAFYHIVIADGRAPRDGKFIEKLGTYNPNT
                     NPATIELDQAKALTWLKNGAQPTETARAILSYKGVLMQLHLDKGVAKGALTEEQAAAK
                     FQKWMDEKTAKIDGKINKLAEGKSDHYKKRLADEAAKNAARAAAIIAKNSPVAEAVEA
                     DSAAEEVSETPEAPAAEETPAAE"
     gene            1377..>2678
                     /gene="lysS"
                     /locus_tag="PK281_11350"
     CDS             1377..>2678
                     /gene="lysS"
                     /locus_tag="PK281_11350"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOY49984.1"
                     /translation="MSEQLSELEIQRRQSLEELINLGINPYPAELFPVSVSAKEVNEK
                     FVKDSGDYQEVSIAGRIMTRRIMGNATFAVLQDSSDRIQIYIRRDDICPGEDKTLYNT
                     VFKKLLDIGDIIGVTGYAFSTQTGETTIHVTSLKVLTKSLRPLPIVKEKDGEVYDAFA
                     DPDLRYRQRYVDLIVNHQVKDTFIKRTQLTNSIRQYLNTCGYLEVETPILQPLYGGAA
                     ARPFKTHHNTLDMTLYMRIANELYLKRLIVGGFDGVYEFAKDFRNEGMSRFHNPEFTQ
                     VELYVAYKDYNWMMDLVEEMVEKVALDIHGKTEVQVGENLINFQRPWKRMTMYEAIHQ
                     YTGVDISEMDEATMAAAARKMNIQIDASMGRGKIIDEIFGETCEPHLIQPTFITDYPV
                     EMSPLAKKHRSKPGLVERFEGICNGKEICNAFSELNDPIDQR"
BASE COUNT          734 a          520 c          548 g          876 t
ORIGIN      
        1 gttttatttc ctgtttactt ccaacaaacc ttcaggtaga ttcatcgaaa tcacacccaa
       61 ttcccgatct actttcacaa tccaatcttc tgcaacagga atgagaactt cttgtccttc
      121 gtttgtgagt tgaaacaggt cgtttggacc attttcaagt acgtctttta tgatgccagc
      181 agataaccca gaagtgagat cttctatttt ccaaccaata atttcgtggt aatagaattt
      241 ggtaccgcta aggggaggaa gtttttcgag cggtagaaaa actaagcagc cagttaatga
      301 atctgcttgt tcggcattat cgacatcttc caatttaata tgtgcagaat tggaaggttg
      361 aagtcggaat gataaaacga aaaacggaac cagttttccg tggagttcca caaatactga
      421 ttccgttttt gtataggctt caggttgatc ggtatcgagt tgcagactta atctgccatc
      481 ggttcctacc ttccgcgtaa agactccaag ctgaaaacac tgttcctgct tcatgagtct
      541 tttataaatt attctgctgc tggtgtttct tctgctgcag gtgcttctgg tgtttcagaa
      601 acttcttcag cagcgctatc agcttctaca gcttcggcaa caggtgagtt tttagctatg
      661 attgcagcag cacgtgcagc attcttagca gcttcatctg ccaaacgttt tttgtagtgg
      721 tctgatttgc cttcagctaa tttgttgatt ttaccgtcga ttttagctgt tttctcgtcc
      781 atccatttct ggaatttcgc agcagcttgt tcttcagtta aagctccttt tgcaacacct
      841 ttatctaagt gtagttgcat caaaacacct ttgtagctaa ggattgcacg tgccgtttct
      901 gttggttgtg ctccgttttt aagccatgtt agtgcttttg cttgatccag ttcgatagta
      961 gctggatttg tgtttggatt gtacgtgcct agtttttcaa taaacttccc atctcgaggt
     1021 gcacgaccat cggcaatcac aatatgataa aaagcataac tctttttacc atgtcgttgt
     1081 aatctgattt ttactggcat ttgttgtttt ttattgattc aaattgggcg gcaaagatat
     1141 aaattttcag attcccaaat gccttgctat tgaatattgt ccatttctaa agtcgaaacg
     1201 ttcatttact tcgggatttt cttgcggcaa gttggtttcg atcctaaaag tacatcatcc
     1261 acaacccttg atattgttgc gataatttga ttttgcacga gaataattca tccaagtaat
     1321 cggcttatta gttcattact atttctcaat tacctttgca gcccgatttt accctcatga
     1381 gcgaacaact ttctgaactt gaaattcaac gacgccaaag tcttgaagag ctaatcaatc
     1441 ttggcatcaa tccttatcct gccgaactat ttcctgtaag tgtttctgct aaggaagtga
     1501 atgagaagtt tgtcaaggac tctggagatt atcaagaagt ctcaattgcc gggcgcatca
     1561 tgacacgccg aatcatgggg aatgcaactt ttgctgttct tcaagattca agtgatcgta
     1621 tacagatata tatacgtcgc gatgatattt gtccgggcga agacaaaaca ctttacaata
     1681 cggtatttaa gaaactactt gatatcggtg atattattgg tgtgacaggt tatgcgttct
     1741 ctacccaaac gggcgaaacg accatacacg ttacttcttt aaaagtattg accaaatccc
     1801 ttcgtccgct tcctattgtt aaggaaaaag atggtgaagt gtatgatgct tttgctgatc
     1861 ctgatttacg ttaccgtcaa cgttatgtgg atttgatcgt gaatcatcag gtaaaagaca
     1921 catttattaa gcgtactcaa ctaacgaact cgattcgtca gtatttgaat acgtgtggat
     1981 atttggaagt agaaacacct attcttcaac cattatatgg tggagctgca gcccgtccgt
     2041 ttaaaacgca tcacaataca ctcgacatga cgctttacat gcgtattgcg aatgagttgt
     2101 atttgaaacg tttaattgta ggtggatttg atggtgttta tgagtttgcg aaagatttcc
     2161 ggaacgaagg aatgagtcgt tttcataatc cagaatttac tcaggtagag ctttatgtag
     2221 cttacaagga ttataactgg atgatggatt tagtggaaga aatggttgaa aaagtagctc
     2281 ttgatattca tggtaaaaca gaagttcaag tgggcgaaaa cttgatcaat ttccaacgtc
     2341 catggaaacg tatgacgatg tacgaggcga ttcaccaata tactggtgtg gatatttcgg
     2401 agatggatga agctactatg gctgctgctg ctcgtaaaat gaatattcaa attgatgctt
     2461 ccatgggtag agggaaaatc atcgatgaaa tctttggtga aacctgtgag cctcatttaa
     2521 tacaacctac tttcattacc gattatccgg ttgaaatgtc accacttgct aagaaacatc
     2581 gtagcaaacc aggtttagtt gaacgcttcg aaggaatctg taacggtaag gaaatttgta
     2641 acgctttctc tgagcttaac gatccgattg atcaacgc
//
LOCUS       DAOOQW010000282         2658 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_46633_length_2658_cov_5.271994, whole genome shotgun sequence.
ACCESSION   DAOOQW010000282 DAOOQW010000000
VERSION     DAOOQW010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2658
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46633_length_2658_cov_5.271994"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            162..917
                     /locus_tag="PK281_11355"
     CDS             162..917
                     /locus_tag="PK281_11355"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOY49985.1"
                     /translation="MKAEDNYIHGMDWTFQHEKGLGSLTNYRSYQYNLVHSFMGKNIL
                     EVGSGDRGFTAEILKSGIKLDRLVSIEPSDGLYAQHQDNFRFPDNVRFDNEDLFNLKP
                     ETYGKFDTVVFIHVLEHIEKHREALDHTATLIEKGGYVLIEVPALPFLFSGHDESLGH
                     YRRYTKKMLREAINPDLYETEKMWYNDPIGVAGSFLFFKVLKRKLNTSEGVSLVKNQG
                     GVYDKYVIPFERKIEKYITFPFGLSLTAVLKRK"
     gene            complement(981..2303)
                     /locus_tag="PK281_11360"
     CDS             complement(981..2303)
                     /locus_tag="PK281_11360"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase, eukaryotic"
                     /protein_id="HOY49986.1"
                     /translation="MPSSLKQKFSEKNSALAANLKSTMAEYGTLKIGEITLGQVYSGM
                     KGMIGMITETSKLDPEEGIRFRGLSIPELRDKLPKAPGGNEPLPEGIFYLMLTGEVPT
                     DFQVRELSADWESRSQVPEHVFKMLDALPIYTHPMTQFSAAVLAMRTDSVFAHEYEKG
                     INKKDYWDPMFEDSMNLIARLPRVASYIYRRTYHNNVQIEPLKGADWSANFAHMMGYD
                     SHEVAELMRLYLTIHADHEGGNVSAHSTHLVGSALSDAYYSFSAGLNGLAGPLHGLAN
                     QEVIRWILELQKDLGGGLPTKEQIEQYVNKTLAEGKVVPGYGHAVLRKTDPRFIAQME
                     FAKKYMPDDDIVNIVWRVYEVVPEILGSIKKIQNPWPNVDAHSGALLIHYGIKEYDFF
                     TALFGVSRALGVLAQLTISRAMGLPIERPGSITTDWIKAKIASSQVNS"
BASE COUNT          812 a          522 c          486 g          838 t
ORIGIN      
        1 aagcggttgt tatcggcata gtactattgt ctgtaatgcc catggttatt ggctatttgc
       61 gacaccgact ttcaaaaaaa taagtcgatt aacgagcaaa cgaacacgtt ttcggccaag
      121 gaattaatac ttacatttga aaaaatcaaa agcacaagag aatgaaagca gaggataatt
      181 atatccatgg aatggactgg acctttcagc acgaaaaagg gctgggctca ttaactaact
      241 atcgttctta tcaatacaac ttggttcatt cctttatggg aaagaatatt ttggaggttg
      301 gaagcggaga tcgtggtttt actgctgaaa ttctaaaatc aggaattaag cttgatcgtt
      361 tggtttctat tgaaccttca gatggtttat atgcccaaca tcaagataac tttcgttttc
      421 ctgataatgt acgtttcgat aacgaggatt tattcaacct aaaacccgaa acatacggca
      481 agttcgatac tgtcgtgttc attcacgtgt tagagcatat cgaaaaacat cgtgaagcgc
      541 ttgatcatac agctacacta atcgaaaaag gtggttacgt tttaattgaa gttcccgcgc
      601 ttccatttct tttttctggt cacgacgaat cactaggaca ttaccgtcgg tatactaaga
      661 aaatgcttcg cgaagccatt aatccggatc tatatgaaac cgagaaaatg tggtataatg
      721 atccaattgg tgttgctggc tcattcttgt tcttcaaagt gctaaaacgc aaactcaata
      781 cttccgaagg tgtttctctt gtgaaaaacc aaggtggagt ttatgataaa tacgtaattc
      841 cgtttgaacg taagattgaa aaatacatca ctttcccatt tggtctcagt cttactgcag
      901 ttttaaaaag gaagtagacc tttataaaaa taaaaaaccc ggatcctagt atagaatccg
      961 ggttttttat tgctgaatct ttaggaattc acttgagaac tggcaatttt agctttaatc
     1021 caatcggttg tgattgaacc gggacgttca attggtaatc ccattgctcg actaatagta
     1081 agttgggcta acacacctaa ggctcttgaa acaccaaata aggcagtaaa gaaatcgtat
     1141 tctttaattc cataatgtat taaaagtgca cccgaatgtg catccacatt gggccaaggg
     1201 ttttgtatct tcttaataga acctaaaatc tctggaacaa cttcatacac gcgccaaaca
     1261 atattcacga tatcatcatc aggcatgtat ttcttggcaa attccatttg agctatgaaa
     1321 cgaggatctg tttttctaag aacagcatga ccgtatccag gaacaacttt tccttcggcg
     1381 agtgttttat ttacgtattg ttcgatttgt tcttttgttg gaagtcctcc gcctaaatct
     1441 ttctgaagtt ccaatatcca acgaattact tcttgattag ccaatccgtg aagtgggcca
     1501 gctaaaccat ttaaacctgc agaaaatgaa tagtaagcat cacttaatgc cgatcctacc
     1561 aaatgggtag aatgagccga aacatttcca ccttcatgat cagcatgtat agttaaatac
     1621 aaacgcatca attcggcaac ctcatgacta tcatagccca tcatatgagc aaaattcgca
     1681 gaccaatcag ctccttttaa tggctcaatc tgaacgttat tatgataagt tcttctgtat
     1741 atgtatgaag caactcgtgg taatcgagca attaaattca ttgaatcctc aaacattgga
     1801 tcccaataat cttttttgtt gatgcctttt tcatactcat gtgcaaatac cgaatcggta
     1861 cgcatggcca aaactgcagc gctaaactga gtcatcggat gcgtataaat tggtaatgca
     1921 tctagcattt taaaaacatg ctcaggtacc tgacttctac tttcccaatc agcactaagt
     1981 tcacgaactt gaaaatccgt tggaacttct cctgtcaaca taagataaaa aataccttca
     2041 ggaagcggct catttccgcc tggggctttg ggtagtttat ctctcagctc tggaatactt
     2101 aatccacgaa aacgaatgcc ttcctctgga tccaatttgg atgtttcggt aatcatccca
     2161 atcatccctt tcatcccaga ataaacttgt cctaaagtaa tctcgccaat tttcaatgtg
     2221 ccgtattcag ccattgtgga ttttaaatta gcagcaagtg cactattttt ttctgagaat
     2281 ttttgcttta aagatgatgg catagaaggt ttaaaaaagg ttatttaaaa agcgctacaa
     2341 gtatactcaa ttcattcccc tactaacagt ttttaattgt atttgttttc catattcgaa
     2401 ggctgataaa tcgatattca ctcttgtttt cctttttaaa agccagtata gaaaatatgc
     2461 tacatttggt tgggtattgt tttattcatt cttatccgta aatgattaat gtcacgaagt
     2521 cgttcctgcc tgatttggag gaatatgtga agtatctaga aggcatctgg gaaagagggc
     2581 acctcactaa tcatggtccg ttggtgctag aattagaatc tcaaattcgc cagtttttgg
     2641 gtgtaaagca cttctttt
//
LOCUS       DAOOQW010000283         2625 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_47451_length_2625_cov_4.873152, whole genome shotgun sequence.
ACCESSION   DAOOQW010000283 DAOOQW010000000
VERSION     DAOOQW010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47451_length_2625_cov_4.873152"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(144..2072)
                     /locus_tag="PK281_11365"
     CDS             complement(144..2072)
                     /locus_tag="PK281_11365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="STN and carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HOY49987.1"
                     /translation="MDRVVKLVLGFLILFGSVLCGQEDPLNRRISIKLAQVSTSDAIQ
                     AIAQKGRFLLSYNASGIDEQKLTSVNAQNKTVRFALEQALGSGYTFRSGGSHVIIIPK
                     TQPKTERRKELYVLSGQIKDAATGKTISSASITEVGEYNSTLSDKQGKYTLPINPETD
                     YARILISRKNYRDTVLILKPEEVHAIEIQLRPLPIISELNPLPASIPIELADNGLFQA
                     VVSEEQQAFSENHPFYENRTFQVSFLPTLGSNRNFSGMIENHLSLNLLGGYSMALSGV
                     ELAGVFNITRKHVKGLQISGLMNITGGEVDGVQIAGFMNNNIGAIRGVQTAGFYNLSM
                     DSLRGVQAAGFFNMARNRVRGVQAAGFMNISGKELSGVQAAGFMNLCGKSGHGSQFAG
                     FANIATGDMYGLQTAGFLNLSTGSMHGAQLTSGVNITVDTTFTVQVAGIANFARWIKG
                     SQVSTLFNIGGKVGGSQLGLVNVCDTIKGISVGFISIVRKGMHQFEISTSDVNQVLFT
                     LRTGTHRFYNVISTGMFRPEDPSFVTFGYGAGTEFRPGKKFYFGIDLVGSMVFNESLK
                     LNVTPDLWGRSGLYLGYRPSKRIGFFAGPTFNVYRIDDSNSTGNRPAIGRDELFTTRQ
                     GTSSLSGWIGWQAGIRLF"
     gene            complement(2056..>2625)
                     /locus_tag="PK281_11370"
     CDS             complement(2056..>2625)
                     /locus_tag="PK281_11370"
                     /inference="COORDINATES: protein motif:HMM:NF027664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4974 domain-containing protein"
                     /protein_id="HOY49988.1"
                     /translation="YSSNYGKPDRKTELKGEAFYDVTRNVNSPFVIQAGEAYIKVLGT
                     SFSINSIQQNDVAIQVSTGKVLVYDGEHEQTSTDSLAVILLPGQEAHFSEAGKPIKTK
                     SIPIENIRYSYDKTLVFENSDLKAVCELLEKIFGAKIQFKREEISNCRLTATFKNQNL
                     TDILLIISETFNITVVKDSQSFILDGPGC"
BASE COUNT          751 a          567 c          476 g          831 t
ORIGIN      
        1 tttccccatc gggtattctt gagcataaag taaagcgcgt actgttctga ttttattttc
       61 ggacatgatg tgtcattttt agaattactt gtacgtacat gaaatttatg ccagtttgca
      121 attcctgaca aactgtctct catctaaaat aatcggatac ccgcttgcca accaatccat
      181 cccgaaagtg aagatgtacc ttgtcttgta gtgaatagtt catctcttcc aattgcgggg
      241 cgattaccag tgctattcga atcatcaatt cggtaaacat taaaagttgg tcctgcgaag
      301 aatccaattc gtttgctggg tctgtaacct aaatacaatc ctgatcttcc ccataaatcg
      361 ggggtgacat ttagtttcaa actctcattg aagaccatcg atccaactaa atcaattccg
      421 aagtagaatt ttttacctgg gcgaaattcg gttccagcac catatccaaa tgttacgaaa
      481 gaaggatcct cagggcgaaa catacctgtg cttataacat tgtagaatcg atgcgttccg
      541 gttctgagtg tgaataatac ttgattcaca tccgaagtag aaatctcgaa ttgatgcatt
      601 cctttacgga caatactgat aaagcctacc gaaattcctt tgatagtgtc gcaaacattc
      661 accaatccta attggcttcc gccgactttt cctccaatat tgaataatgt agaaacctgg
      721 gaacctttta tccagcgggc aaaattggca attccagcta cttgaacggt aaaggttgta
      781 tcaactgtaa tatttactcc cgatgttagc tgtgcaccat gcatgcttcc tgtgcttaag
      841 ttgaggaatc cagcagtttg caaaccatac atgtcgccag ttgctatatt ggcaaaacct
      901 gcgaattgag aaccatgtcc tgatttacca cacaagttca tgaatccagc agcctgaact
      961 cccgataatt ccttgcccga aatattcatg aatccagcag cttgaacacc tcggacccga
     1021 tttctagcca tgttaaagaa tccagcagcc tgaacacctc ttaatgaatc catggaaaga
     1081 ttataaaatc ctgcagtttg aacaccccga atggctccaa tgttattatt catgaagcca
     1141 gcgatttgaa ctccatccac ttctccaccg gtaatattca tcaagccact tatctgcaag
     1201 cctttcacat gttttcgagt gatattaaac actccggcta actcaacacc acttaatgcc
     1261 atcgaatatc ctcccaataa attcaaagaa aggtggtttt caatcatccc tgaaaaattc
     1321 cggttgctgc ctaaagtggg aagaaatgat acctgaaaag ttcgattctc ataaaaaggg
     1381 tgattctcag aaaatgcctg ttgctcttct gatacaaccg cttgaaataa tccgttatcg
     1441 gctaattcaa ttggaatgct agctggaagc ggatttagtt ccgaaattat tggaagtgga
     1501 cgcaattgta tttctatcgc atgaacttct tccggcttca aaatcaaaac ggtatcccga
     1561 tagtttttcc gactaattaa tatgcgagca tagtctgttt cgggattgat tggtaaggtg
     1621 tatttgcctt gtttatcgct caatgtggaa ttgtattctc ctacttccgt gatagatgcg
     1681 gatgaaattg ttttcccagt agcggcatct tttatttgtc cactcaacac atacagctct
     1741 tttcttcgct ctgttttggg ttgtgtttta gggatgatga tgacatgact tcctcccgaa
     1801 cgaaacgtat aacccgaacc taatgcttgt tcaagggcaa aacggacagt tttattttga
     1861 gcatttactg aagtaagctt ctgttcatca attcctgaag cattgtaact tagcagaaac
     1921 cgaccttttt gagcaatcgc ttgaattgca tctgaagtgc tgacctgtgc taacttaatc
     1981 gaaatccttc tatttaatgg atcttcctgt ccacaaagga cagaaccgaa caagattaaa
     2041 aaacctagaa caagtttaac aacccggtcc atctaatata aaggattgag aatctttcac
     2101 tacggttatg ttgaacgttt ccgaaatgat caataatata tcagttagat tctgattttt
     2161 gaaagttgct gttaatctac aattggagat ttcttcgcgt ttaaattgga ttttagcccc
     2221 aaagattttt tctaataact cgcaaactgc ctttaaatca ctgttctcga agacaagggt
     2281 tttatcataa ctatatcgga tgttttctat tggaatcgac ttggttttta tgggtttgcc
     2341 agcctcagaa aaatgagctt cttgtcctgg tagtaatatc actgccaacg aatcggttga
     2401 ggtttgctcg tgttctccat catatacaag cacttttccg gtgctcactt gaatcgccac
     2461 atcattttgc tgaattgaat ttatactaaa tgaagttccg agtactttga tatatgcttc
     2521 acccgcttga attacgaatg gactattcac attgcgtgtc acatcataga aagcttctcc
     2581 tttcaattct gtttttctat ccggttttcc ataattggat gaata
//
LOCUS       DAOOQW010000284         2602 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_48061_length_2602_cov_5.504908, whole genome shotgun sequence.
ACCESSION   DAOOQW010000284 DAOOQW010000000
VERSION     DAOOQW010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48061_length_2602_cov_5.504908"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1098
                     /gene="atpD"
                     /locus_tag="PK281_11375"
     CDS             <1..1098
                     /gene="atpD"
                     /locus_tag="PK281_11375"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009745944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HOY49989.1"
                     /translation="IDLIEPYSKGGKIGLFGGAGVGKTVLIQELINNIAKNYGGLSVF
                     AGVGERTREGNDLLREFIESDVINYGKEFKHSMEEGGWDLSKVDKKSLEESKATLVFG
                     QMNEPPGARARVALSGLTMAEYFRDGDEQSGGRDILFFIDNIFRFTQAGSEVSALLGR
                     MPSAVGYQPTLATEMGQMQERITSTKRGSITSVQAVYVPADDLTDPAPATTFAHLDAT
                     TVLNRKISELGIYPAVDPLDSTSRILSRDVVGDAHYDTAQRVKETLQRYKELQDIIAI
                     LGMDELSEEDKLTVSRARRVQRFLSQPFFVATQFTGLEGRFVQIEDTIKGFNMIMDGE
                     VDEYPEAAFNLVGTIEEAIEKGKKLLAESAN"
     gene            1110..1346
                     /gene="atpC"
                     /locus_tag="PK281_11380"
     CDS             1110..1346
                     /gene="atpC"
                     /locus_tag="PK281_11380"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010182116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HOY49990.1"
                     /translation="MHLEIISPDKKLYEDEVTTLTLPGIDGSFGILDKHAPLISALKK
                     GKIKVTDKASARHVFEINGGVVEVLNNKVIVLAE"
     gene            1428..1709
                     /locus_tag="PK281_11385"
     CDS             1428..1709
                     /locus_tag="PK281_11385"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOY49991.1"
                     /translation="MEYTLVDNSAEQQFEFLLEDGEKAFVTYRIKEGVVYLLHTSVPE
                     HFEGKGIASQLAKKVFSHLKESGTLAKLYCAYLRTYVERHPEWEIILVA"
     gene            1718..2182
                     /locus_tag="PK281_11390"
     CDS             1718..2182
                     /locus_tag="PK281_11390"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018280035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HOY49992.1"
                     /translation="MVSDEFYMGEALREAKKAFAADEVPIGCVIVWKDRIIARAFNRT
                     EQLKDFTAHAEMQAFTSASDYLNNKYLNECTLYVTLEPCVMCSGASFWTQLGKIVFGA
                     SDLKRGVSAISSRILHPSTEVVSGVMEQECGTLLSEFFQKKRKKDSGTPLQD"
     gene            2300..>2602
                     /locus_tag="PK281_11395"
     CDS             2300..>2602
                     /locus_tag="PK281_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009413136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOY49993.1"
                     /translation="MQQIRNIAIIAHVDHGKTTLVDKILHQTKLFRENQQFGELILDN
                     NDLERERGITILSKNVSVRYKDVKINIIDTPGHADFGGEVERVLNMADGVLLLVDAF"
BASE COUNT          776 a          506 c          595 g          725 t
ORIGIN      
        1 atcgacttga tcgagcctta ttcaaaaggt ggtaaaatcg gtcttttcgg tggtgccggt
       61 gtaggtaaaa cagtattgat tcaggagttg atcaacaaca tcgctaagaa ctacggaggt
      121 ttatctgtgt ttgcgggtgt tggtgagcgt actcgtgaag gaaatgactt attgcgcgag
      181 ttcatcgaat cggacgttat caactatggt aaagagttca agcattcgat ggaagaaggc
      241 ggatgggatc tttctaaagt ggataaaaaa tcattggaag aatcaaaagc taccttggtg
      301 ttcggtcaga tgaacgagcc tccaggagca cgtgctcgtg tggcactttc tggtcttact
      361 atggcggaat atttccgtga tggtgatgag caatcaggtg gacgtgatat ccttttcttc
      421 atcgataaca tcttccgttt cacgcaagca ggatctgagg tatcggcact tttaggtcgt
      481 atgccatcag cggtaggtta ccaaccaact ttggcaacag agatggggca gatgcaggag
      541 cgaatcactt caactaaacg tggatctatt acctctgtac aagcggtata cgtacctgcg
      601 gatgacttga cggatccggc accggcaaca acatttgccc acttggatgc tacaacggta
      661 ttgaatcgta aaatttctga gcttggtatt tacccagctg tggatccact cgattcaact
      721 tcacgtatcc tttctcgtga tgtagttggt gatgcacatt acgatactgc acaacgcgta
      781 aaagaaacac ttcaacgtta caaagaactt caagatatca tcgccatcct tggtatggac
      841 gagctttctg aagaagataa attgactgta agtcgtgcac gtcgtgttca acgtttcctt
      901 tctcaaccat tctttgtagc aactcagttt acaggtttgg aaggtcgttt cgttcaaatc
      961 gaagacacca tcaaaggttt caacatgatt atggatggag aagtggatga atatccagaa
     1021 gctgcattca acttggttgg aacaattgag gaagcgattg aaaaaggtaa aaaactattg
     1081 gctgaatcgg ctaattaatc caatcgcaca tgcatctgga gatcatatca cccgataaaa
     1141 agttgtacga agacgaagtg acaaccttaa cccttccggg gattgatgga tcatttggta
     1201 ttctcgacaa acatgctccg cttatctctg ccttgaaaaa aggaaagatt aaagtaactg
     1261 acaaagcatc tgctcgtcac gtatttgaaa tcaatggagg agtggtagaa gtgttgaaca
     1321 acaaagtgat tgttcttgct gaataatcgt aactaataat atcgaaaggc tcacccgaaa
     1381 cggtgggcct ttttttattc caatgattat cttcaccaaa aatatatatg gaatacacct
     1441 tagtagataa cagcgcggag caacaatttg aatttctctt ggaagatgga gaaaaggcat
     1501 tcgtcaccta tcggataaag gaaggagtag tttatttgtt acatacatcg gttccagaac
     1561 atttcgaagg aaaaggaatt gcttctcagt tagctaagaa agtgttcagc catttgaaag
     1621 aatcgggcac tttggcaaaa ttgtattgtg catacttgag aacctatgtg gaacgtcatc
     1681 ccgaatggga aatcatttta gtagcctaaa ctgaaaaatt gtgagtgacg aattttacat
     1741 gggagaagct cttcgtgagg cgaaaaaggc ctttgcagcc gatgaagttc ctattggatg
     1801 cgtgattgtg tggaaagacc gaattattgc ccgagccttt aatcgtaccg aacagctaaa
     1861 agattttact gctcatgccg aaatgcaggc atttacaagc gcatccgatt atctgaataa
     1921 taaatacctg aatgaatgca cgctttatgt gactttagaa ccctgcgtga tgtgcagcgg
     1981 agcttcgttt tggactcaat taggtaaaat tgtatttggg gcaagtgatc tgaaacgagg
     2041 tgtttcggct atctcttcac gcattttaca tccttcaacc gaggtggttt cgggtgtgat
     2101 ggagcaagaa tgtggcacat tactcagcga attcttccag aagaaaagaa agaaagattc
     2161 cggaactcct ttacaggact aatttcccac cgagttcatc tccccataaa cagctcattc
     2221 aatttttaac attttgctct gagccgaatg ctgctttagt cagtaaaaat gactactttt
     2281 gcgccgccaa aaacctgaca tgcagcaaat acggaatatt gcaattattg cccacgttga
     2341 ccacggtaag acaaccctcg ttgacaaaat tctacatcaa acgaaactgt ttcgcgagaa
     2401 tcagcagttc ggagaattga ttcttgataa taatgacctc gaacgtgagc gcggaattac
     2461 gattttgtcg aaaaacgtat ccgttcgata caaagatgtt aaaatcaaca tcattgatac
     2521 accaggccac gccgactttg gcggagaagt agagcgtgta ttaaatatgg ctgatggggt
     2581 tcttttattg gttgatgcct tt
//
LOCUS       DAOOQW010000285         2594 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_48260_length_2594_cov_5.626625, whole genome shotgun sequence.
ACCESSION   DAOOQW010000285 DAOOQW010000000
VERSION     DAOOQW010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2594
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48260_length_2594_cov_5.626625"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1432
                     /locus_tag="PK281_11400"
     CDS             <1..1432
                     /locus_tag="PK281_11400"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HOY49994.1"
                     /translation="SRIYRLMFELDLSYSILLVVCLIAACAFEFVNGFHDTANAVATV
                     IYTNSLKPWVAVIWSGFWNFLGVFWGGIAVAVGIINLLPVETLVDQNIAHSLAMVLAL
                     LITAIFWNLLTWYFGIPCSSSHTLIGSILGVGLAYSLLPEASGDAVNWGKAQDIGLSL
                     LVSPFFGFSATIILMYLIRTFTKKTESGESLFKEPKKNQPPPLWIRGILVITCTLVSF
                     FHGSNDGQKGVGLIMLILIGVVPAFFALNANFTPSDLPQSLQKIENVISTIDSTKLSE
                     PDRKKLVESITMNERLETRFASLRDINDIPKQERFLVRKDIMVMDRNLNSLAKKDEVK
                     LSEAEKKTVKDELKKVRGVTDYSPTWVLLLISLSLGLGTMIGWKRIVKTVGEKIGKEH
                     LTYAQGASAEIVASITIGLSSNYGWPVSTTHVLSSGIAGSMVANKGVKNLQPATIRNI
                     LAAWFLTLPVVMLMAGTLFLLFRSIF"
     gene            1488..2483
                     /locus_tag="PK281_11405"
     CDS             1488..2483
                     /locus_tag="PK281_11405"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HOY49995.1"
                     /translation="MKTLFYILAIVTLISCAQKEMKTIGTIERIDPALDAIIQGEPKV
                     EVIAEGFKWSEGPIWIKEQNLLLFSDVPTNTIYKWTAEKGKEVYLTPSGYTGTSTHYS
                     NEPGSNGITLNSDGKLVLCQHGDRRVALMDAPLTAPEAKFITLVDNYQGKKLNSPNDA
                     VFRSNGDLFLTDPPYGLMKQENDSTREIPFQGVFKVSNGVASLITDSLTRPNGIAFLP
                     GEKTLIVANSDPGKATWYAFDLLENDSVANARIFYDATENTKTEKGLPDGLKIDKQGN
                     VFASGPGGIWIFNKDGKVLGKIKISEPTSNCALADDDKTLYATSNMYVVRIKLRD"
BASE COUNT          775 a          548 c          547 g          724 t
ORIGIN      
        1 gagccgaatt taccgactta tgttcgaact agacctgtcc tactccatcc tccttgttgt
       61 atgtcttatt gctgcctgtg cgttcgaatt cgtcaatgga ttccacgata cagccaatgc
      121 tgtggcaacc gtaatttata ccaactcact aaagccttgg gtagcggtaa tctggtcggg
      181 attctggaat ttccttggtg tgttctgggg aggtattgct gttgcagtag gtatcatcaa
      241 cctgttacct gtagaaacac tggtcgatca aaacattgcg catagtttgg cgatggtact
      301 cgccctatta atcactgcta ttttctggaa cttgctcacc tggtactttg gtattccatg
      361 ctccagctct cataccctta ttggttctat attaggtgtc ggacttgcat actccttatt
      421 accagaagca tcaggtgatg cggtgaactg gggaaaagct caggacattg gactgtcatt
      481 gttggtttct ccgttttttg gattctccgc taccatcatt ttaatgtatc tcattcgaac
      541 attcacaaaa aaaacggaat caggagaaag cctgtttaaa gagccaaaga aaaatcaacc
      601 accaccactg tggattcggg gtatactcgt aatcacttgc acgttggtga gtttctttca
      661 cggatcaaac gatggacaaa aaggtgtagg ccttatcatg ttgatcttaa taggagttgt
      721 gcctgccttc tttgcattga atgcgaattt cactccctct gatttacccc aatctttaca
      781 gaaaattgaa aatgttattt caacaatcga ttctaccaaa ctatcggaac cggatcggaa
      841 aaagttagta gaatccatta caatgaatga gcgtttggaa accaggtttg cttcactacg
      901 cgatattaat gacattccaa agcaagagcg tttccttgtg cgtaaagata ttatggtgat
      961 ggatcgcaac ttaaattcac tcgctaaaaa agatgaagta aagttgagtg aagcggaaaa
     1021 gaaaaccgtg aaagacgaac tgaaaaaagt acgtggcgtt acagattact ctcctacctg
     1081 ggttcttctt ctgatctcac tctcgcttgg cctcggcacc atgattggtt ggaaacgtat
     1141 cgtgaaaact gttggtgaga aaatcggaaa agagcacctt acctatgcac agggagcaag
     1201 cgcggaaatt gttgcatcca taaccattgg tctctccagc aactatggat ggccggtaag
     1261 tacaacccac gtattatcat cgggaattgc cggaagtatg gtggcgaata aaggcgttaa
     1321 aaatcttcaa cctgcaacca ttcgaaatat tctggcagcg tggttcttaa ccttacctgt
     1381 tgtaatgctc atggccggga cattattcct gttattccgc tccattttct agaatcgttt
     1441 tttactctgc aaaagtcatc ttattttcag gaaaataaat cctgaatatg aagactcttt
     1501 tttacattct tgccatcgtg acattaatct cgtgcgcaca aaaagaaatg aaaaccatcg
     1561 gaaccattga gcgaatcgat ccggcattag atgcgatcat tcaaggtgaa cctaaagttg
     1621 aagtcattgc tgaaggtttt aaatggagtg aaggtccaat ttggataaaa gaacaaaatc
     1681 tgcttttgtt ctctgatgtt cccaccaaca cgatttataa atggacggcc gaaaaaggaa
     1741 aagaggttta ccttactcct tccggataca ctggcacatc aacacactac agcaatgagc
     1801 ccggctccaa tggaattacc ttaaacagcg atgggaaact tgtgctttgc cagcatggcg
     1861 atagacgagt tgcattgatg gatgccccac tcacagcgcc tgaagccaag ttcataacac
     1921 tcgttgataa ctatcaagga aaaaaattga acagcccgaa tgatgcagtg tttagaagca
     1981 atggcgattt gtttttaact gaccctcctt atggattaat gaaacaagaa aatgattcta
     2041 ctcgcgagat tccgtttcaa ggtgtcttta aagtttcgaa tggcgtagcg agtttgatca
     2101 ccgattcact tacgcgtcct aacgggattg catttttacc gggagagaaa acactcatcg
     2161 ttgcaaactc cgatcctgga aaagcaacct ggtatgcatt cgatcttttg gaaaatgatt
     2221 cagtagcaaa cgcacgaata ttttatgatg ccactgaaaa caccaaaacc gaaaaaggat
     2281 tacccgatgg tttaaaaatt gacaagcaag gaaatgtttt cgcgagcgga ccaggcggca
     2341 tctggatttt caacaaagac ggtaaggtat taggtaagat taaaatatca gaacccacat
     2401 caaactgcgc tctggcagat gatgacaaaa cactttatgc tacttctaat atgtatgtgg
     2461 tgcgaatcaa gctgcgggat tagatgaatc ttacaaaata aatttccatg actttagggg
     2521 aagcccgaat catcgtaacc gaactcacaa aaagcccaag cttactccgc cacatgcgaa
     2581 gtatagaatt ggta
//
LOCUS       DAOOQW010000286         2552 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_49350_length_2552_cov_5.381258, whole genome shotgun sequence.
ACCESSION   DAOOQW010000286 DAOOQW010000000
VERSION     DAOOQW010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49350_length_2552_cov_5.381258"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1308)
                     /locus_tag="PK281_11410"
     CDS             complement(<1..1308)
                     /locus_tag="PK281_11410"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOY49996.1"
                     /translation="MILRFSSFFLFLLISANTFAQSQEDSLFIRRIFDQALTDSRCYQ
                     VLEHLCTKVPSRLSGSPGAAQAVAYMLQVMKEEKFDVSSLQPVMVPHWVRGQESRVEV
                     RQKGAPVKKLQSCALGGSIGGKVSAGVIEVQQFEDLEKLGEAGIKGKIVFFSRPMEPR
                     HIHTFEAYGGCVDQRWAGPSKASKYGAVATLVRSVSLREDEFPHTGSMAYDSLYPKIP
                     ALAISTADASWLSAALKKNSSLRLEVESFSKLLPDTLSHNVVAQLTGSSKPNEFLITG
                     GHLDAWDNGQGAHDDGAGCVQSMEAIRILHELGYKPRHSMRAVMFMNEENGLRGGKRY
                     AELADSLQEKHIYAIETDRGGFTPRGFYIDSDNPETVAKIAAYRSLLEPYGLHDFKKG
                     GGGADIGPLKKQGTVCLGYIPDSQRYFDVHHAASDTFDKVNKRE"
     gene            complement(1383..1646)
                     /locus_tag="PK281_11415"
     CDS             complement(1383..1646)
                     /locus_tag="PK281_11415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49997.1"
                     /translation="MSLTIEQQNELNRLNEVFRRFDSQMNASDVQADDLVHQFMMEEF
                     PLVNPGKAHMQKLADMRRSILEKRNDSPIQQLDASGDIQLSFE"
     gene            1828..>2552
                     /locus_tag="PK281_11420"
     CDS             1828..>2552
                     /locus_tag="PK281_11420"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HOY49998.1"
                     /translation="METIQTEITGSIATIRLNRGKANAINAQMANELLDTLLELKAND
                     QVRGVLLTGHGEFFSAGLDVIELYHYDENQIIDFWKVFDRLIRRLVSFPKPLVAAITG
                     HCPAGGCILTLGCDYRVMAEGKYRIGLNEIPLGIVVPETIYHLYSFVIGRGHAYQSLL
                     EGKLHTAEEALAIGLVNETCDLAEVEARGKVQLEKYLQLSTAVWAQSKLNFRNAILHR
                     LTSDFKTAFEPTLKQWWSPETRA"
BASE COUNT          776 a          532 c          532 g          712 t
ORIGIN      
        1 ttctcgcttg ttaaccttat cgaaggtgtc ggaagcagca tgatggacat caaaataacg
       61 ctgtgaatca gggatatatc ctaaacaaac cgttccttgt tttttgagcg gaccaatatc
      121 ggctccgcca ccaccttttt tgaaatcatg aagtccgtat ggttctaata aagaacgata
      181 agcagcaatt ttagctactg tttcaggatt atctgaatcg atataaaatc cacgtggtgt
      241 gaatccaccg cgatcggttt cgatggcata aatgtgcttt tcttgcaatg aatcggctag
      301 ttcggcatac cgtttcccgc cacgcaaacc attttcctca ttcataaaca taacggcacg
      361 catcgaatga cgaggcttat aacccagttc gtgtaggatt cgaattgcct ccatactttg
      421 tacacaaccg gctccatcgt cgtgagcacc ttgtccatta tcccaagcat ctaaatgtcc
      481 gccagtaata aggaattcat tgggtttgga agaaccggta agttgcgcaa ccacattatg
      541 ggagagtgtg tcaggaagta gtttggagaa actttcgacc tctaaacgaa gtgaagaatt
      601 tttctttaat gcggcactta accaagatgc atcagctgta ctaattgcaa gagctggaat
      661 ctttgggtaa agtgaatcgt aagccattga accagtatgc ggaaactcat cctcccgcaa
      721 agaaacactt cgaactaagg ttgcaaccgc gccatatttg gatgctttgg atggtccagc
      781 ccaacgttga tctacacatc caccatatgc ttcgaatgtg tgaatatgac gaggctccat
      841 tggccgacta aaaaacacga ttttcccttt aattcctgct tctcccaact tttctaaatc
      901 ttcaaattgt tgaacttcaa tcacaccagc agagactttt ccaccaatac ttccgcctag
      961 ggcacaggat tgtagttttt taacaggagc tcctttttgc cttacttcta ccctactttc
     1021 ttgtccgcgc acccaatgtg gcaccattac aggctgcaag ctagagacat cgaatttttc
     1081 ttccttcata acttgtagca tataagctac agcttgagca gctccgggcg aaccacttaa
     1141 tcgagaagga acttttgtac ataaatgctc tagtacttgg tagcagcggc tatcggttaa
     1201 agcctgatcg aaaatccgac gaataaagag ggaatcttcc tgagattgag caaaagtgtt
     1261 tgcagaaatc agcaggaata aaaagaagct agaaaagcga agtatcatgg aagtaaatgc
     1321 ttgggcaaaa aaaaatccgg aagaacgggt ctttcggatc gaaatatcgg aagtatcggg
     1381 atttattcga aactcaattg aatatctcca ctagcatcta attgctgaat cggagaatcg
     1441 ttgcgttttt cgagaatgga gcgacgcata tcagctaact tctgcatgtg tgctttacca
     1501 ggattaacaa gtggaaattc ttccatcatg aactgatgta ctaaatcatc tgcctgcaca
     1561 tcagatgcat tcatctgaga atcgaaacga cgaaacactt cattcaggcg gttcaattcg
     1621 ttttgttgtt caattgtgag tgacatgttg gattgtttat acatcctctg ataatgaagc
     1681 taatagcaac aggatgcaga tttggttcta acaaaagtaa caggactttt taaatatgac
     1741 aagcatatga cattcagtta tcaacaatgt aggttaagaa ccttgtcagt agccacaatt
     1801 aggtattttt acccttttag catacgtatg gaaaccattc aaacagagat taccggatca
     1861 atagccacca ttcgattaaa tcgtgggaaa gccaatgcca tcaatgcaca aatggcgaat
     1921 gagcttttag ataccttgtt ggaattaaag gcaaatgatc aagttagagg tgtattacta
     1981 accggacatg gagaattttt ctcggcagga ttagatgtta tagaactgta tcattacgac
     2041 gaaaaccaga ttattgattt ttggaaagta ttcgatcgtt taattagaag attagtttcc
     2101 tttcccaaac cacttgtagc agcaataaca ggccattgtc cggctggtgg ttgcatatta
     2161 actttagggt gtgattatcg ggtgatggca gaaggcaagt accgaattgg attaaatgaa
     2221 attccacttg ggattgttgt cccagagacg atataccatt tgtacagctt tgtgatagga
     2281 agaggacatg cgtatcaaag tctgttagaa ggaaaacttc atacagcaga agaagcttta
     2341 gcaattggat tagtgaatga aacctgcgac ttggccgaag tagaagcgcg aggaaaagtt
     2401 cagttagaaa aatacctcca actcagcacc gccgtttggg ctcagagtaa actcaatttc
     2461 cgtaatgcga ttcttcaccg cttaacatcc gatttcaaaa ccgcattcga acctacctta
     2521 aaacaatggt ggtcgcccga gacacgagct at
//
LOCUS       DAOOQW010000287         2552 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_49352_length_2552_cov_4.870645, whole genome shotgun sequence.
ACCESSION   DAOOQW010000287 DAOOQW010000000
VERSION     DAOOQW010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49352_length_2552_cov_4.870645"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(156..875)
                     /locus_tag="PK281_11425"
     CDS             complement(156..875)
                     /locus_tag="PK281_11425"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY49999.1"
                     /translation="MSKKKESIYVLLEGECISVYFLESPGSSPITPLTQVLKIDSMGV
                     HSVDECFEFLKRYNLSIDRYFYGISGHVKKNFPRHYFKIRFNGAHNLGAKAIKIKLAQ
                     WHVDQAERYRSGYSILTNFKTDEFKAVEWYKKALEWWPHPGAMLSLAYCYAFGARTEK
                     QVNQAIELFERVTLVDATYFHKQSDRKYYIEIIQGEAYYYLGYIFKIGLGIPIDLAKS
                     QGYFQLGKELKNRRCEEELMT"
     gene            complement(934..2472)
                     /locus_tag="PK281_11430"
     CDS             complement(934..2472)
                     /locus_tag="PK281_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013552355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HOY50000.1"
                     /translation="MLTKVFGSAVSGINATTITVEVTVTQGIKFFLVGLPDSAVKESQ
                     QRIESALLLNGYRMPGKKIVVNMAPADIRKEGSSYDLTIAIGIAAASGQMPAERLSEF
                     IIMGELSLDGGILPIRGALPIAVQARKEGYKALILPAENAAEAAIVSDLAVYGASNLS
                     EVIRFLSGELDLEPVVVDPEKEFAAANLFSEVDFSDVKGQENIKRAMEIAAAGGHNVI
                     LIGPPGAGKTMLAKRLPSILPAFTLEEALETTKIHSVAGKLGKHTSLLSSRPFRNPHH
                     TVSDVALVGGGGHPQPGEISLAHNGVLFLDELPEFKRTVLEVMRQPLEDRIVTISRAR
                     STVEYPASFMLIASMNPCPCGFYNHPEKECVCSPGVVQKYLNRVSGPLLDRIDLHVEV
                     TPVKFDELTTVRNSEPSSSIRERVIQARAIQKERFEEVPGVHCNAQMNSRMLRNWCKI
                     GPDGNQLLKTAMERLSLSARAYDRILKVSRTIADLEGSPQIGLAHISEAIQLRSLDRE
                     NWAG"
BASE COUNT          733 a          576 c          486 g          757 t
ORIGIN      
        1 ctctaggcct atcagcagag aaagcaatcc acataaaagc agatttaata catctgccgg
       61 aattcgacta atgagttcca tttgttaaaa ataggcaatc attcttcata aacccatgat
      121 attagtagcg tttccttcaa tataacccga aaaaattagg tcattaattc ttcttcgcac
      181 ctcctattct ttaactcttt tcccaattga aaatagcctt gtgatttggc taaatctata
      241 ggaattccca agcctatttt gaatatataa cctaaataat aataagcttc tccttggatg
      301 atttcgatgt agtatttcct gtcggattgt ttatgaaaat acgtcgcatc cactagtgta
      361 actcgttcaa acaattcgat agcttgattt acttgctttt ctgttctcgc accgaaggca
      421 taacaatagg ctaacgacag cattgctcca ggatgtggcc accattcgag tgctttttta
      481 taccattcaa ctgctttgaa ctcgtcggtt ttaaagtttg tcaaaatcga atatccacta
      541 cgataccgtt ctgcttgatc aacatgccat tgagccaact ttattttaat cgcctttgct
      601 cccaaattgt gcgctccatt aaatcggatt ttgaaatagt gacgagggaa attctttttt
      661 acatgtcctg atattccata gaaataacgg tctattgaca aattatatcg cttcaaaaat
      721 tcgaagcact catcaaccga atgaactccc atcgaatcaa ttttcagcac ttgtgttagg
      781 ggagtaatcg gacttgaacc agggctttcc agaaagtaaa ccgaaatgca ttcaccttct
      841 aataacacat aaatcgattc ctttttcttg gacattatca tcaacagatc aagaaacaaa
      901 tatactctta gctcgatttt aaacttgatt tcctcaacca gcccaatttt ctcgatctaa
      961 acttctcaac tgaatagctt ccgaaatgtg agctaatcca atttgaggcg aaccttctaa
     1021 atcagcaatc gttcgtgaaa cctttaaaat tcgatcatac gctcgtgctg aaagtgaaag
     1081 cctttccata gctgttttga gtaattgatt tccatcggga cctattttgc accaattgcg
     1141 tagcattcgt gaattcatct gtgcattgca atgtactccg ggcacttctt cgaaacgctc
     1201 tttttgtatg gctcgtgctt gtattacgcg ttcgcggatg cttgagctag gttcggaatt
     1261 tcgaacggtg gttaattcat caaactttac tggagtaact tctacatgca aatcaatccg
     1321 atctaacaag ggtcctgaaa cacggttgag gtatttttgt accacacccg gactgcatac
     1381 acattctttc tccggatgat tgtaaaatcc acacggacaa ggattcatag atgcaatcag
     1441 cataaaactg gcaggatatt caactgttga acgggcgcgc gaaatggtca caatccgatc
     1501 ttccaagggt tgacgcatta cttccagtac cgtgcgttta aattcgggta gttcatctaa
     1561 gaatagaact ccattatggg ccagtgaaat ctctcccggc tgaggatgtc cgccgccacc
     1621 aacgagtgct acatcagaaa ctgtatgatg cggatttcgg aatgggcggg aagagagtaa
     1681 tgacgtatgt ttcccgagtt tgcctgctac cgaatgtatt ttggttgttt ctaaagcctc
     1741 ttccaatgtg aaagcgggaa gtatagacgg aagtcgtttg gcaagcatgg ttttcccagc
     1801 tccgggtgga ccaatcaaaa tgacattatg ccctcctgct gcggcaatct ccatagctcg
     1861 tttgatgttt tcttgtcctt tcacatccga gaaatccact tcagaaaata gatttgcagc
     1921 agcaaattct ttttcgggat ctaccacgac tggttctaaa tctaattcac ccgataaaaa
     1981 gcgaattacc tccgaaagat tacttgctcc atatacggct aagtcgctaa caatagccgc
     2041 ttcagctgca ttttcggcag gtaaaatcaa ggctttgtat ccttcttttc tcgcttgaac
     2101 ggcaatgggc aatgctccac gaatgggtaa aattccgcca tcaagggata attctcccat
     2161 gatgataaat tccgacagcc gctcggcagg catttgtcca gatgctgctg caatgccaat
     2221 agctattgtt aaatcgtagg aagaaccttc ttttcgaata tcggcaggtg ccatattcac
     2281 aacaattttc ttcccgggca tgcggtaacc attgagaagt agagccgatt cgattcgttg
     2341 ctgactttct tttacggcac tatcgggtaa tcctacaagg aagaatttaa ttccttgagt
     2401 tacggttact tcaaccgtaa tggtggtggc attaataccc gaaacggcac tgccaaaaac
     2461 tttggtaagc atgatattcc atcaaacagc gacacaaaac ccggcgctat tcaggtacgg
     2521 aacaaacgaa aacggaacaa gatgcaagaa cg
//
LOCUS       DAOOQW010000288         2536 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_49789_length_2536_cov_5.272874, whole genome shotgun sequence.
ACCESSION   DAOOQW010000288 DAOOQW010000000
VERSION     DAOOQW010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49789_length_2536_cov_5.272874"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..872)
                     /locus_tag="PK281_11435"
     CDS             complement(<1..872)
                     /locus_tag="PK281_11435"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HOY50001.1"
                     /translation="MIELGKFSLPVLYLVYIAGMFLISLVINGILIKFSMNLGAKNED
                     NQVRWASTTKPAFGGVSFFLLFLVSIVFFAIFFGNGQILLNAQFLGLLTASSLGFLMG
                     LADDAYNTKPFLKFAVQFLCALLFILTNNCIQTFPWEWLNITLTFLWVVGMMNSINML
                     DNMDAITASVSTVICIGLAMILVFRGDFLNLDLVLLLGVIAGIGGFLYYNWNPSRMYM
                     GDSGSQFLGAFLAGMSIHFLWNAPDYYGQLIPEKQILLVALLFIVPISDTTTVSINRL
                     KRGQSPFVGGRDHTT"
     gene            complement(884..1435)
                     /gene="rfbC"
                     /locus_tag="PK281_11440"
     CDS             complement(884..1435)
                     /gene="rfbC"
                     /locus_tag="PK281_11440"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HOY50002.1"
                     /translation="MRIEQVPISGLMIISPNVFEDPRGYFFESYSKKAFEEAGITEEF
                     VQSNQSLSQKGVLRGLHFQHPPHAQSKLVRVIQGSVLDVALDIRKGSPTYGQHFSIVL
                     SAQNKTMFYVPVGFAHGFLTLEDNTIFSYKCGNYYNKPAEDGILWNDSDLNIEWNVEN
                     PILSEKDKVNTLFRDFNSPFTHP"
     gene            complement(1438..>2536)
                     /locus_tag="PK281_11445"
     CDS             complement(1438..>2536)
                     /locus_tag="PK281_11445"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HOY50003.1"
                     /translation="IEFVRLQKLNEISERYYNRLVEIKSELTIYNAGLTSENTVLETS
                     STPVSPYFPSRRIVALGALIGWILVSLGLVVIRYLFHNEITSLNEIMKYLEVPMLGIV
                     PNYKDVIPISQLLVDKKPKSIIAESLRSIRSNLEFLSHTDGPKVLAITSTISGEGKTF
                     VALNLAGIIAFSEKKVIILDLDMRKPKIHVGFGVPNDKGMSTILINRNSIDDCIFKSS
                     LNNLDFITAGPVPPNPSELIISSRMLEVIQELKTKYDVIVIDNPPVGLVTDGIRVIKM
                     ADYPIYVFRENFSKRNFVQNVKKLIRDNNIKNMSIILNSVDIKRSGYGYSGVYDYDYG
                     YGYGYGFGYYDEDIRREKFSLKEFFRKIISK"
BASE COUNT          855 a          477 c          459 g          745 t
ORIGIN      
        1 gtagtatggt ctcgaccgcc aacaaaggga ctttgaccac gttttaaacg gttaatcgat
       61 acggttgttg tgtcactaat tggcacaatg aaaagtagag caacgagaag aatttgtttt
      121 tcggggataa gttgaccgta gtagtcgggt gcgttccaga ggaagtgaat tgacattcca
      181 gctaaaaatg ctcctaagaa ttgtgagcca ctatcgccca tgtacattcg agaaggattc
      241 cagttataat aaaggaaacc tccaatccct gcaataactc caagaagtaa tactaaatcg
      301 aggttaagga aatcacctcg aaatactagg atcatggcta aaccaatgca tattacagta
      361 gaaacgctag ctgtaatagc atccatatta tcaagcatat tgatggaatt catcattcca
      421 acaacccata aaaaggttag tgtaatattt aaccactccc atggaaacgt ttgaatgcaa
      481 ttatttgtca agatgaacaa caatgcgcac aaaaactgaa cagcaaactt gaggaatggt
      541 ttggtattgt atgcatcatc agctagtccc ataaggaaac caagtgaact agcagttaga
      601 agacctagga attgggcatt tagaagaatt tgtccatttc caaagaaaat ggcgaaaaat
      661 actatggaca cgagaaacaa aaggaagaaa gaaactcctc caaaagcagg tttggtagtt
      721 gaagcccaac gtacttgatt atcttcgttt ttagctccca aattcattga gaacttaatt
      781 agaattccat tgattacaag ggaaatcagg aacattccgg caatataaac cagataaaga
      841 acaggtaagg aaaattttcc cagctcaatc attctttcag gtattatgga tgagtgaagg
      901 gtgaattaaa gtcgcgaaat agcgtattta ctttgtcttt ttcagataga ataggatttt
      961 ccacattcca ctctatgttt aaatctgagt cgttccagag aataccatct tcagcaggtt
     1021 tgttataata attaccgcat ttataagaga aaatggtgtt gtcttcaagc gttaaaaatc
     1081 cgtgagcaaa cccaacagga acataaaaca tggttttgtt ttgcgcactc agtacaatcg
     1141 aaaaatgctg tccataagtt ggagaacctt ttctaatatc caaagctaca tctagcacag
     1201 aaccttgaat tactcgtaca agtttggatt gagcgtgagg tgggtgttga aaatgcaaac
     1261 cacgcaaaac acctttttgg gaaaggcttt gattactttg gacaaactct tccgtgatac
     1321 cagcttcttc aaacgctttt ttactgtatg attcgaagaa atatccacgt ggatcctcaa
     1381 acacattggg tgaaataatc atcaaccctg aaataggtac ttgctcaatt ctcatactta
     1441 tttacttatg atttttcgga agaattcttt gagactgaat ttctcacgac ggatatcttc
     1501 atcataatat ccaaaaccgt aaccgtaacc gtagccataa tcgtaatcat aaactccact
     1561 gtaaccatag cccgaacgct tgatatcaac agagttaagg ataatcgaca tgtttttgat
     1621 gttattatct cgaattagtt tttttacatt ctgaacaaaa tttcgtttgg agaaattctc
     1681 cctaaaaacg taaatcggat aatcagccat tttaatcact cgaattccgt cagtcaccaa
     1741 tccaacaggt ggattatcaa tcactatcac atcgtattta gttttcaatt cttggattac
     1801 ttccaacata cgtgaactaa taattagttc agaaggattt ggaggaactg ggccagcagt
     1861 aatgaaatcc aagttgttca aactcgattt gaatatacag tcatcaatcg aatttctatt
     1921 gatgaggatt gtactcattc ctttgtcatt aggaacacca aaaccaacgt ggattttagg
     1981 tttcctcatg tctaagtcga gaatgataac tttcttctcc gaaaatgcga taatccctgc
     2041 aagattcagg gctacaaagg tttttccctc acccgaaatt gtagatgtaa ttgctaaaac
     2101 ttttggtcca tcagtatgag aaaggaattc gagattggat cgaatggaac gaagtgattc
     2161 agcaatgatg gactttggtt ttttatccac caataattgc gaaattggaa taacgtcctt
     2221 gtaatttgga acgataccta acataggtac ttcaagatac ttcatgattt cattcaacga
     2281 agtaatctca ttatggaata aatatcggat tacaactaat cctaaactca ctaatatcca
     2341 acctatcaaa gctcctaatg ctactatacg gcgactagga aaatagggag aaacaggagt
     2401 tgatgaagtt tcaagtacag tgttttcaga tgttaatcct gcattgtaaa tagttaattc
     2461 cgacttgatc tcaaccaatc tattgtaata ccgttcactg atttcattta atttctgaag
     2521 tcgaacaaac tcaata
//
LOCUS       DAOOQW010000289         2528 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_50023_length_2528_cov_4.261626, whole genome shotgun sequence.
ACCESSION   DAOOQW010000289 DAOOQW010000000
VERSION     DAOOQW010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50023_length_2528_cov_4.261626"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /locus_tag="PK281_11450"
     CDS             complement(<1..651)
                     /locus_tag="PK281_11450"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOY50004.1"
                     /translation="MNILVRISLFLLVSTLHVSAQVHIEDYHTGQLYVRLNESFPLAT
                     SRIPEREIATFFDSYQVQFGITEVKAAFWFSKSASNRIYRISFNQRALTEQFIKQLEA
                     SGKVIYAERIPISRKALVPNDLGPNSSATGGQWYLYKIKAQQAWDIQAGGVASVKVGV
                     VDDAVDYTHPELSGVILPGYDIASDDFDVMPPDPNWDHGTHICGLIGANTNNSAGMA"
     gene            complement(670..1527)
                     /locus_tag="PK281_11455"
     CDS             complement(670..1527)
                     /locus_tag="PK281_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HOY50005.1"
                     /translation="MIHITECPRDAMQGIHTFIPTAEKISYLQQLLKVGFPVLDAGSF
                     VSPKAIPQLADTHEVFDALDMSATQTKILAIVANARGASEAVAVDKVSILGFPFSVSE
                     TFQQRNTNSSIAQSLSRVEEIIELCASRNKELVIYLSMAFGNPYGDEWSPELVAKWAK
                     TLEGMGVKNLSLADTTGISEIQGISTLYTTIQAELSSANLSMHLHSLPQDVALKAEAA
                     YNAGCRYFDSALKGFGGCPMASDTLTGNMATEVLIQWANVNQIETGINHLELKEAERQ
                     ANFLFDRYL"
     gene            complement(1511..>2528)
                     /locus_tag="PK281_11460"
     CDS             complement(1511..>2528)
                     /locus_tag="PK281_11460"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HOY50006.1"
                     /translation="FLFSPEKAHAISVRLFKILLYVPGFEGIVRWYSNGFDRRLRLDK
                     KGLRFPNRVGLAAGFDKNATFFQDFRHLGFGFIEVGTVTPLAQNGNPQPRLFRLTEDE
                     AIINRMGFNNLGVEAMTERLKKRPAGLIIGGNIGKNKLTPNEHAVSDYLICFRELQPY
                     VDYFAVNVSSPNTPGLRALQEKEPLKALLNALQEEARHMGADQPILLKIAPDLNEQEL
                     LDIVEIVQECELAGVIATNTTISRTGLKTSAEKIEKIGAGGLSGKPVKNRSTEVIKFL
                     REHLGSEPLIIGVGGIHSADDAWEKLNSGADLIQLYTGFIYEGPSLIRAIHQKCIANH
                     DTYN"
BASE COUNT          754 a          531 c          478 g          765 t
ORIGIN      
        1 agccatgcct gctgaattat tcgtattggc tccaattaac ccacaaatat gtgtcccatg
       61 atcccaattc ggatcgggtg gcatgacgtc aaaatcgtcg gatgcaatat catagccggg
      121 taaaataact cccgataatt caggatgagt ataatctact gcatcatcta caactccaac
      181 ttttaccgat gcaacgcctc ctgcttgtat atcccaagct tgttgcgctt taattttata
      241 caaataccat tgtccgccag ttgcactcga attaggacct aaatcattcg gaactaaagc
      301 tttccttgaa attggaattc gctccgcata aatcactttt cctgaagctt ctaattgctt
      361 aataaactgt tcggttaaag ctctttggtt aaaggaaata cgataaatac ggtttgaggc
      421 tgattttgaa aaccaaaaag ctgcttttac ttctgttatt ccaaactgaa cttgatagga
      481 atcaaaaaat gtagctattt ctcgttctgg aattcgtgaa gttgctaaag gaaacgactc
      541 gttaagtcta acatacaatt gtccggtatg ataatcttca atatgaactt gagccgatac
      601 atgaagtgtc gacacaagta aaaacagaga aatacgaacg agtatgttca tgcaagtgta
      661 atattctgat taaagataac gatcaaaaag gaaatttgct tgtctttcgg cttccttcaa
      721 ctccaaatgg ttaatgccgg tttcaatttg attaacattt gcccattgaa ttaatacttc
      781 ggtagccata tttccggtaa gtgtgtcgct tgccatggga caaccaccaa aaccttttaa
      841 agctgaatcg aaataccggc aaccagcatt ataggctgct tctgccttca aagcaacatc
      901 ttgtggaagg ctgtgaagat gcatggataa gttggcagat gataattccg cttggatagt
      961 agtatagagg gttgatattc cttggatttc tgaaattccc gttgtgtcgg ctaaggatag
     1021 attcttaaca cccattcctt ccaaggtttt agcccatttt gcgaccagtt cgggcgacca
     1081 ttcatcccca tatggatttc caaatgccat ggagagataa atgactaact ctttatttcg
     1141 gctggcacag agttcaataa tttcctctac ccgactgagt gattgagcga tcgatgaatt
     1201 tgtatttcgt tgttgaaagg tttcagaaac cgaaaaagga aatcccaaaa tgcttacttt
     1261 atccactgct acagcttctg atgcaccacg ggcatttgct acaatagcaa ggatttttgt
     1321 ttgagtagcc gacatatcaa gggcatcaaa aacctcatga gtatcagcta attgaggaat
     1381 tgctttgggt gaaacgaaac tccccgcatc caaaactgga aatccaactt ttagcaactg
     1441 ctgtaagtag gaaatttttt cggcagttgg aataaatgta tgaatgcctt gcatcgcatc
     1501 acgcggacat tcagttatat gtatcatggt tagctatgca tttttgatga atggcacgta
     1561 ttagtgatgg gccttcatag atgaaacctg tgtacaattg aattaagtcg gcacctgaat
     1621 taagcttttc ccaagcatca tcagctgagt gaattcctcc cacaccaatg attagtggtt
     1681 cgcttcctaa atgttcgcgc aaaaacttta tgacttcagt agaacgattt ttaactggct
     1741 ttccacttaa tccaccagct ccaattttct caattttttc agccgaagtt ttaagtcctg
     1801 tgcgacttat cgtagtatta gttgcaatga cacctgccaa ttcgcattct tgaacaattt
     1861 caacgatatc aagcaattcc tgctcattca aatcaggagc aattttcaat aatatcggtt
     1921 gatcagcacc catatgtcga gcttcttctt gcaaagcatt taataatgct tttaatggtt
     1981 ctttctcttg taatgcgcgt aaacccggtg tgttaggtga gctaacgttt acggcaaaat
     2041 aatctacata aggttgtaat tctcgaaagc aaattagata atcacttaca gcatgttcgt
     2101 taggtgtgag tttattcttt ccaatatttc ctccgatgat taaacctgct ggacgctttt
     2161 tcaatcgctc ggtcatggcc tcaactccca aattattaaa acccatgcgg ttaataatgg
     2221 cttcatcttc ggtcaaacga aaaagtcgcg gctgtggatt tccattttga gctaatggag
     2281 ttaccgttcc aacttcaatg aaaccaaaac cgagatgacg aaaatcttgg aagaatgtag
     2341 cattcttatc aaatccagct gctaatccta cgcggttggg aaagcgaagt ccttttttat
     2401 ccaaccttaa gcgccgatcg aacccattcg aataccaacg aacaattcct tcaaatccag
     2461 gcacataaag cagaatctta aacaatcgaa ctgagatggc gtgtgctttt tcaggagaaa
     2521 aaaggaaa
//
LOCUS       DAOOQW010000290         2518 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_50319_length_2518_cov_3.419001, whole genome shotgun sequence.
ACCESSION   DAOOQW010000290 DAOOQW010000000
VERSION     DAOOQW010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2518
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50319_length_2518_cov_3.419001"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            64..933
                     /locus_tag="PK281_11465"
                     /pseudo
     CDS             64..933
                     /locus_tag="PK281_11465"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
     gene            927..1544
                     /locus_tag="PK281_11470"
     CDS             927..1544
                     /locus_tag="PK281_11470"
                     /inference="COORDINATES: protein motif:HMM:TIGR03570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HOY50007.1"
                     /translation="MLIVGAGGFAKQLLPLIDRLDLSKQVVFFDDYSNDTTSLISRNF
                     HVIRSEEELKQYFAHGDLRFVNAVGGPVNRLKIHQKMQGLGAEPISLIDPDSSISQYE
                     VEIGTGCCILQQVVIEPGVSIGKGCLINLNSLLTHDVSIGDFSEISPSVTILGGAKIG
                     HSTFVGAGAIILPKIEIGDNCVVGAGAVVNRSVPSGHRVVGVPAR"
     gene            1556..2503
                     /locus_tag="PK281_11475"
     CDS             1556..2503
                     /locus_tag="PK281_11475"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOY50008.1"
                     /translation="MRLISFILPVFRNKGSISITCEKLASLMHESHTDLDFEIILIND
                     GSDDGSWEEIQEVSQLNPRIKALSFSRNFGQVPAIIAGTQHCTGDAAVIMSADLQDPV
                     SLITDMIAAWKDNSQIVVCYRMDREDSVMANMTSAVFYRFMKIANPKMPKGGFDFVML
                     DRAAIDVFASIDERNRFFQGDILWMGFNIRFLPYKRIKRTIGKSQWSFGKKLKYFIDG
                     SLNTSYMPIRFMSIAGIGTAFIGFLYAFIVLYARMINQIPFKGYAPIVILILVIGGMI
                     MIMLGIIGEYLWRIYDETRKRPLYIVKDKAGNWEEKPKN"
BASE COUNT          727 a          444 c          494 g          853 t
ORIGIN      
        1 cttctttttt gtaaataatg gtactattgc ccttcaaata gctataaagc agctggttta
       61 tcgggtgatg ttattacaac tcctttctct tatgtagcaa ctacgagttg ccttgtttgg
      121 gaaaattgta agcctgtttt cgctgatatt gatccgcttt cgcttacggt tactgctcaa
      181 actatcgaaa aagttatcac acctcaaact acaggtattt taattaccca tgtttatgga
      241 aacccatgtc cagttgagga aatcgaagca ctcgcaaaac gtaaaggtct taaagtaatt
      301 tacgatgcag ctcacgcttt tggtgtagag tataataatc agtctgtttt aaattatggt
      361 gatatttcca ctttaagctt tcatgcaact aagcttttcc acacaatcga aggtggcgga
      421 attattacga atgatgatga attagcccat aagataagtt acatgcgtaa tttcggacac
      481 aatggaccag aagcattttt tggtgttggt gtaaatggga aaagttctga gtttcaagca
      541 gcaatgggct tgtgtttaat gcccaaaatc aatcagataa tagaatctcg taaatcactt
      601 tctgatctgt atacttccag tttagctgga ttaccaattc aacacttgaa aattcaagcc
      661 ggtacaacta aatacaatta tgcatattac ccaattgtat ttcaaacaga aaccgatttg
      721 cttaaagtag ttaaggcgct gaacgagaaa caaataatcc ctagacgata tttctatcca
      781 tcgttgaata cattaaatta tgtgacgaat aatttagttg acgattatgc ttcagaaatt
      841 tctaaaagag tgctctgctt accactttat cccgacttgg caagcgaaca cattcttacc
      901 atttccgatt gtatccgttc tcaaatatgc tgatagttgg tgctggtggt tttgcaaaac
      961 aacttctacc tctaatcgat cgattagatt taagtaagca ggttgttttt tttgatgatt
     1021 atagcaatga taccacctca cttatttcac gtaatttcca tgttattcgt agcgaagagg
     1081 aattaaaaca gtatttcgct catggtgatt tgcgttttgt taatgctgtt ggcggtcctg
     1141 taaatcggtt gaaaatacac caaaaaatgc aaggtttggg tgccgaaccc attagcttaa
     1201 ttgatccaga ttctagtatt tcccagtatg aagttgaaat tggtacaggt tgttgtattc
     1261 ttcaacaggt tgttatcgaa cccggagttt ccattggtaa aggttgttta attaatctaa
     1321 attcacttct cactcacgat gtttctatag gtgatttttc ggaaatttct ccaagcgtta
     1381 ccatactggg aggtgcgaaa atagggcatt cgacctttgt tggggctggt gctattattc
     1441 ttcccaaaat agaaataggt gataattgtg tagttggtgc tggggcagtc gtaaaccgtt
     1501 ctgtaccttc ggggcatcgt gttgtaggtg ttcctgctcg ataaacttgt tacatatgcg
     1561 attaatttcc ttcattcttc ctgtattccg aaacaaaggc tctataagca ttacctgcga
     1621 aaagcttgct tcgttaatgc acgaatcaca tactgatttg gattttgaaa tcatccttat
     1681 taacgatggt tcagatgatg gatcttggga agaaattcaa gaagttagtc aacttaatcc
     1741 tcgaattaaa gctctttcct tttctaggaa ttttggacaa gtgccagcta taattgctgg
     1801 aactcaacat tgtactggcg atgccgctgt tattatgtcg gccgatttgc aagatccagt
     1861 aagcctaatt actgatatga ttgcggcttg gaaggataat agtcaaatcg ttgtttgcta
     1921 tcgaatggat cgtgaagatt cagttatggc taacatgact tcagccgttt tctatcggtt
     1981 catgaaaatc gcaaacccta aaatgcctaa aggtggtttc gatttcgtta tgctagatcg
     2041 agctgctatt gatgtttttg catctattga tgaaaggaat cgcttttttc agggggatat
     2101 tctttggatg ggattcaata ttcgtttcct tccatataaa cgaataaagc ggacaattgg
     2161 gaaatctcaa tggagttttg gtaaaaagct taaatacttc atcgatggtt cccttaatac
     2221 atcttatatg cctattcggt ttatgtctat tgccggaata gggactgctt tcatcggctt
     2281 tttatatgct tttattgtcc tttatgctcg catgattaat caaattcctt ttaaaggata
     2341 tgcacctata gttattctaa tattagtaat tggaggtatg attatgatca tgctaggtat
     2401 cattggagaa tacctatggc ggatttatga tgaaactcgt aaacgtcctt tgtatatcgt
     2461 caaggataaa gcaggcaatt gggaagaaaa acctaaaaat taatatccac ttacaagt
//
LOCUS       DAOOQW010000291         2500 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_50828_length_2500_cov_3.662577, whole genome shotgun sequence.
ACCESSION   DAOOQW010000291 DAOOQW010000000
VERSION     DAOOQW010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50828_length_2500_cov_3.662577"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..595
                     /locus_tag="PK281_11480"
     CDS             <1..595
                     /locus_tag="PK281_11480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY50009.1"
                     /translation="LEGLAYRLIPIKSPSGRGDNFGRINTSLMYDNMMNKFAWGGVDK
                     KEVYMDENNIRMTMNLRSNFDRLASALLQEGKKDSAIKVVDRAFEVLPERNVPYNFFV
                     VQLAEDYYQAGVNDKADKVLKRYADIIEQELVYYYSQKPKIMKKFDSEIQRNMSIFKF
                     MLDVARRGKRDELVKDFDARFRKLESRYVPVAGGGQN"
     gene            602..1225
                     /locus_tag="PK281_11485"
     CDS             602..1225
                     /locus_tag="PK281_11485"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004569711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOY50010.1"
                     /translation="MSLVQPTGLLRKLMPGAIWRMPASGKVVYLTFDDGPTPDVTNQV
                     LAMLQTANASATFFCIGKNIVANPDLFSQITLLGHQVGNHSFSHKNGWKTDFQSYLSD
                     VQECDKWVKTGLFRPPYGRLSIRQFIYLRKRYRIILWDVLSMDYDVKLSPDQCLKNVL
                     NHVRPGSIITFHDSVKAWPRLKEILPLVLSDLTSQGYRFETLFPIKF"
     gene            1256..2410
                     /locus_tag="PK281_11490"
     CDS             1256..2410
                     /locus_tag="PK281_11490"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOY50011.1"
                     /translation="MDKETETGYFDPIRPFYDHEVSEAIDRLLQQASFHTALRYVFPD
                     VSSEDVQLRLRSLKSVDEFQSKIISNAVAAIIRSSTSGVRSSGLDKLDPKKAYLFISN
                     HRDIVLDSALLNYLLHMDEFPTTRIAIGSNLLQKSWIEDLVKLNKNFIVHRNVQARQA
                     YDYSLRLSRYIRHSITKENSSVWIAQREGRSKDGYDQTQAGLLKMFGMSTDDGPAGYQ
                     ELNIVPVSISYELEPCAGMKAFEKYTKAQTGSYEKGEGEDLKSMQMGIARPKGRVQFS
                     FGEPLSKEEIQLAFETGNRNEAMKKMAERIDSHIVSNYRLYPYNYIAYDLLHESKEFV
                     SNYSDKDKLAFESYLNEELLPLKGDAELLKKHLLNIYANPLVRKKEMKLI"
BASE COUNT          776 a          442 c          497 g          785 t
ORIGIN      
        1 attggaaggt ttagcttacc gattgattcc tattaagagt ccatctggca gaggcgataa
       61 ttttggtcgt atcaatacct cgctcatgta cgataacatg atgaacaaat ttgcttgggg
      121 tggtgtagat aagaaagagg tttatatgga tgaaaataac attcgtatga ccatgaatct
      181 ccgcagtaat ttcgatcgtt tagcttcagc attgcttcaa gaaggaaaga aagattcagc
      241 cattaaagtg gttgatcgtg ctttcgaggt tttacctgaa cgaaatgttc cttacaattt
      301 ctttgtagtt cagttggcag aagattatta tcaagcaggt gtgaacgaca aagccgataa
      361 ggtgttgaaa cgctatgccg atatcatcga acaagaattg gtatactact acagccagaa
      421 accgaaaatc atgaagaaat tcgattcgga aattcagcgt aacatgtcca ttttcaaatt
      481 catgttggat gttgcacgaa gaggaaaacg tgatgaattg gtgaaagatt tcgatgctcg
      541 tttccgaaaa ctcgaaagta gatatgttcc cgttgctggc ggcggacaga attaatttac
      601 catgagcctt gtccaaccga ctggcttatt aagaaaacta atgcccggag ctatttggcg
      661 tatgccagct tcgggcaaag ttgtttatct cacatttgat gatgggccaa cacccgatgt
      721 aaccaatcag gttctagcta tgctgcagac tgcaaatgcc tctgctacct ttttttgtat
      781 tggaaaaaat attgttgcta atcccgattt attctcacaa ataactttgt taggtcacca
      841 agttggaaat cattcttttt cccataaaaa cggctggaaa actgattttc agagttattt
      901 atccgatgtc caagaatgcg ataaatgggt aaaaaccgga ttatttcgtc cgccttatgg
      961 tagattatct atccgtcaat ttatttatct gcgtaagcgt tatcgaatta ttctatggga
     1021 tgttttatcg atggattatg atgtgaaact cagtccagat caatgtttga agaatgtctt
     1081 aaatcatgtt cgtccaggtt ccattattac ctttcacgat tcggtgaaag cttggcctcg
     1141 cttgaaagaa atattacccc ttgtattaag cgatttaact agtcaaggct accgatttga
     1201 aacactcttt ccaattaaat tttgatgtaa ttaaaaaact tcgttctttg cccacatgga
     1261 caaggaaaca gaaacgggat attttgatcc catccgccct ttttacgatc acgaagtatc
     1321 ggaagcaatt gatcgtttat tgcaacaagc ttcatttcac actgcactgc gatatgtatt
     1381 tccggatgtg agtagtgaag atgttcaact tcgtttgcga tcacttaaat cggtagatga
     1441 gtttcagtca aagatcattt caaatgctgt tgcagctatt attcgtagtt ctacatcagg
     1501 cgttcgctca tcgggtttag ataaactcga tcctaagaag gcttacctat ttatttccaa
     1561 ccatcgagat attgttcttg attcagcttt gttgaattac ctattacata tggatgagtt
     1621 tcctacaact cgaattgcca ttggtagtaa tcttctacaa aaatcttgga ttgaagattt
     1681 agttaaactg aataagaatt tcattgtaca tcgcaatgtt caggctcgtc aagcttatga
     1741 ctattcacta agattatcac gttatatccg tcattccatc actaaagaaa attcatctgt
     1801 atggattgcc caaagagaag gacgttcgaa agatggatat gatcaaactc aggctggttt
     1861 attgaaaatg tttggaatga gtaccgatga tggtcctgcc ggatatcaag aattaaatat
     1921 tgttccggtt agtatctctt atgaattaga accttgtgct ggaatgaaag cttttgagaa
     1981 atatacaaaa gcacaaacgg gttcctatga gaagggtgaa ggcgaagatt tgaagagtat
     2041 gcaaatggga atcgctcgtc caaaaggtcg ggtacaattt agttttggtg agcctttatc
     2101 taaagaagaa attcagcttg cttttgaaac tggaaacaga aatgaggcta tgaagaaaat
     2161 ggctgaacgc atcgattcac acattgtttc taattatcgt ttatatccat ataattatat
     2221 tgcatacgat cttttacacg aatcaaaaga attcgtaagc aattattcgg ataaagataa
     2281 attggcattt gaatcctatc tcaatgaaga gttgttacca ttgaaagggg atgccgaatt
     2341 attgaaaaaa cacttgctta atatttacgc aaatccctta gtgcgaaaaa aggaaatgaa
     2401 attaatttaa ttactatccg ataaatccaa ttttacggtg attcccatcg caaaatggtt
     2461 tattacttga tgctccacaa cggcataatg caacggttcc
//
LOCUS       DAOOQW010000292         2484 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_51294_length_2484_cov_4.684232, whole genome shotgun sequence.
ACCESSION   DAOOQW010000292 DAOOQW010000000
VERSION     DAOOQW010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51294_length_2484_cov_4.684232"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="PK281_11495"
     CDS             complement(<1..501)
                     /locus_tag="PK281_11495"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOY50012.1"
                     /translation="MENPPLQLLKQCRGNDRKAHFSLYEWCFNDLISICRRYYKNDDE
                     LKSAVNMSFLKLIQSMDTIIQKYDELVFYHWMKRITVNHIIDEFRKNKKYREQIDLRE
                     QDELLRNTEDLGAEEYTWKENLETIQRAIDSLPEMSKTVFNLYAIDGYKHEEIGELLN
                     ISANTSK"
     gene            complement(556..1974)
                     /locus_tag="PK281_11500"
     CDS             complement(556..1974)
                     /locus_tag="PK281_11500"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOY50013.1"
                     /translation="MKKLLTLALGLILSSTMLNAQFLCGFVYQGDPTTGTYYFSADPA
                     SGDSLAYMYQWTFNNGSVVVNGPYAQNQYDQSTVDVVTLTIYNTSDSSIYCTGTQEVV
                     VTVDSSSGSECPISYQVSPANPNTYIFWVPGANYPATWTFDNGTTTTSGNEVPFTFSD
                     PGYHTICMNITGGGFTCNDCVEIFIPSDSTMTPGCSADFWASTSALVGYYIPTGYNYS
                     NTATYSWNFGDGQSSTEMYPYHEYTTSGNYEVCLTVTDGSCSENSCQPVFIPESNTVP
                     TDSSCYAGFVVTQDNPFEVTVVNAASGLDLNFVWTLSSNGISITSEGAFPTIEVDSTG
                     GFVFCLTVSNGNGCEATYCDSIIVGPNGNIGGRMRSNGFTINIVSPQVLTGYTLGMEE
                     SNEVAFNLYPNPFSEVLNLNTAGKEFKSYSIYSVDGKRVQAGSINGTVQTLNTSDLSQ
                     GVYVLTLTDAKGNSQVQKVVKK"
     gene            complement(2116..>2484)
                     /locus_tag="PK281_11505"
     CDS             complement(2116..>2484)
                     /locus_tag="PK281_11505"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOY50014.1"
                     /translation="TGSFEVRLQVQNEFGCTDDALQTIHIGGFTAFYIPKAFTPGNSD
                     GLNDVFLPKSTGMAPTGFEMSIYDRWGHLVFYSDSWEKGWDGTIDGKPVPMDTYVCKI
                     RYYDKLGNGNDHIGAVTVTD"
BASE COUNT          787 a          474 c          453 g          770 t
ORIGIN      
        1 tttggatgtg ttggctgata tatttaataa ttcacctatt tcttcgtgtt tatagccatc
       61 aattgcatac agattaaata cagttttact catttctgga aggctatcta ttgctcgttg
      121 tattgtttct aaattttctt tccaggtata ttcttcggct cctaaatctt ccgtatttcg
      181 gagtaattca tcttgttctc gtaaatcaat ttgttctcgg tattttttgt ttttccggaa
      241 ttcatcgatg atgtgattga ctgtaattcg tttcatccag tgataaaaaa ctaattcatc
      301 gtatttctgg attatggtat ccatggattg aatgagcttt aagaaactca tgtttaccgc
      361 tgattttaat tcatcgtcgt ttttataata cctacgacaa atgctaatca gatcattaaa
      421 acaccactca tacaaagaaa aatgggcctt tcggtcattt cccctgcatt gcttaagaag
      481 ctgaagtggt ggattttcca tgaatgaaaa aaagctcctc gccacaccga cgaggagcta
      541 tgattgagag agaacttatt ttttcacaac tttctgaact tgactgttgc ctttagcatc
      601 tgtcaaagtc aacacataaa caccttgact taaatcagaa gtgttaagtg tttgaactgt
      661 tccattgatt gaaccagctt gaacacgttt tccatctaca gaatagattg aatacgattt
      721 gaattcttta cctgctgtgt tcaagttcaa tacttcgctg aatggatttg gatataggtt
      781 gaaagctact tcattgcttt cttccatacc taaagtgtat ccagttaata cttgcggact
      841 tacaatgttg attgtaaatc cattagaacg catacgtcca ccgatgttac catttggacc
      901 aacaatgatt gaatcgcagt aagtagcttc acaaccattt ccattagaaa ctgtcaagca
      961 gaatacgaaa ccaccagttg aatcaacttc gatagtaggg aaagcacctt cggatgtgat
     1021 agaaattcca ttagaagaaa gtgtccaaac aaagtttaag tctaaacctg aagctgcatt
     1081 aacaactgtc acttcaaatg gattgtcttg ggtgacaacg aaaccagcgt agcaagaaga
     1141 atcggttgga acagtattac tttctggaat aaatacaggt tggcaagagt tttcagaaca
     1201 agaaccatca gtaacagtta aacaaacttc atagtttcca gaagtagtgt attcatgata
     1261 tggatacatt tctgttgaag attgaccatc accaaaattc caagaatatg tagcagtatt
     1321 tgagtagtta taacctgttg ggatgtaata accaactaaa gcagacgtag aagcccagaa
     1381 atcagcactg caacctggag tcattgttga atcgctagga atgaagattt ctacacaatc
     1441 gttgcaagtg aaaccacctc ctgtaatgtt catacagatt gtatgataac caggatctga
     1501 aaatgtaaat ggaacttcgt ttccagatgt agttgttgtt ccattgtcga aagtccatgt
     1561 tgcaggataa ttggcacccg gtacccaaaa aatgtaggta tttggatttg ctggagatac
     1621 ttgatacgaa attgggcatt cactaccgct tgacgaatca actgtaacaa caacttcctg
     1681 agttcctgta caataaatag aagaatccga agtattataa atggtaagag ttacaacatc
     1741 aactgtagat tgatcgtatt gattttgagc atatggacca ttaacaacta cagatccatt
     1801 attaaatgtc cattgataca tgtaagccaa cgaatcacct gaagcaggat cagcagagaa
     1861 ataatacgtt cctgttgtag gatcgccttg gtaaacaaag ccgcacaaga attgcgcgtt
     1921 tagcatagta ctggacaaaa tcaagcccag agcaagagtt aggagttttt tcatgttttt
     1981 ttagatttaa cgttctgccc tcatacacgg ttgtgtttaa aaaagggttt acacgcaaat
     2041 ctacaaaaca atccagctta gtccactgaa ctcattatca gctaaataat ttcagaaaat
     2101 aattcaaata accagttaat cggtcacggt tactgcacca atatggtcgt ttccgtttcc
     2161 gagtttatcg taataacgga ttttacacac ataggtatcc atcggtacag gttttccatc
     2221 aatggttcca tcccaacctt tttcccagct atcggaatag aatactaaat gtccccagcg
     2281 atcgtaaatg ctcatctcaa atcctgtagg agccattcct gttgatttag gaaggaaaac
     2341 atcatttaaa ccatccgagt ttccaggagt gaatgcttta ggaatataga aagcagtaaa
     2401 tccgccaata tgaatggttt gtagggcatc atcggtacaa ccaaattcat tttgtacttg
     2461 aagtctaact tcgaaagaac ctgt
//
LOCUS       DAOOQW010000293         2470 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_51733_length_2470_cov_4.556936, whole genome shotgun sequence.
ACCESSION   DAOOQW010000293 DAOOQW010000000
VERSION     DAOOQW010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51733_length_2470_cov_4.556936"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..791)
                     /locus_tag="PK281_11510"
     CDS             complement(<1..791)
                     /locus_tag="PK281_11510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY50015.1"
                     /translation="MIKHSRFRFLSFLLIGTGILFQSCSTEENTAWDIDVLGPLAETH
                     LSVADIVADSLIISEPGAPVVIRYVSKYNLIPNDSLYQIPDTAFNNSFSLPLTVNLPA
                     GFQVLNLSQLIKFAYKDAQLTEAVIESGTATFEVQSMLGDKVYFNYSIPKAFKNGIPL
                     NLYDEEIQAGSEQSPSSVQKTIDLSGYYLDLRGDNGTLANQLKVNLDAKLNPTGTGAA
                     AIANQALFNYTNKFIAIRPHYARGYLGSSSFTGSDYLNLSDMRKLS"
     gene            complement(887..1741)
                     /locus_tag="PK281_11515"
     CDS             complement(887..1741)
                     /locus_tag="PK281_11515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY50016.1"
                     /translation="MKAIPFSLRASALAAATLLISFSSCRKDSDNDSDTNASKNESVA
                     ERYFNELNDISDQAAKTGDLSSFKLAADEGVLLSNCATITFDTSGTVSATNPDTIIID
                     FGTSCLGNDLKTRSGKIIISTTGRYRDQGTVITITPLNYFVNNNQILGTRTVTNTGNN
                     AQGQPTYTVQVDGSIVLANNGGTITWTASRVRTWTEGFNTPLLFSDDVFSVTGSSNGT
                     KVSGDTWTSQITSPLVHKRSCHQTVSGTLVVTPSNRPQRTVDFGSGDCDNQVTVTING
                     NTYTINIQ"
     gene            complement(1804..2277)
                     /locus_tag="PK281_11520"
     CDS             complement(1804..2277)
                     /locus_tag="PK281_11520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOY50017.1"
                     /translation="MRNGTDAGVLSEAGAPAIADPGASLLALAHDMGIPVVPIPGPSA
                     IMLSIMGSGLNGQQFTFHGYLPIDKIARTRKLRELEQISFQTGYAQFFIETPYRNTAL
                     LEDCFQVLNPATRLCIGVNLTLQDGWIRTIPVSRWKQNKPAINKLPAVFGILKSS"
BASE COUNT          751 a          454 c          515 g          750 t
ORIGIN      
        1 gaaagttttc gcatatcaga caaattcaaa taatcacttc cagtaaatga actacttcct
       61 aaataaccac gggcatagtg ggggcgaatc gcaataaact tattggtata attgaacaat
      121 gcttgattag ctattgctgc agcacctgtc cctgttgggt taagtttagc atctaagttt
      181 acttttaatt gattggctaa ggtgccatta tcaccacgca aatcgaggta ataaccactt
      241 aaatcgatgg tcttttggac agatgaaggt gattgttcac ttcccgcttg aatctcttca
      301 tcatacaaat tgagtggtat tccatttttg aatgctttag gaatgctgta attaaagtat
      361 actttgtcac ccaacatact ttgaacttca aatgtggcag tgcccgattc aattacagct
      421 tcggttaatt gagcatcttt ataagcaaac ttgataagct gagaaagatt caatacctga
      481 aatccagctg gaagatttac agttagtggt aaactaaaag agttgttaaa agctgtgtca
      541 ggtatctgat agagtgaatc gttaggaatc aaattgtatt tactcacata acgaatcact
      601 acaggagcac caggctcgct aataatgagc gaatcggcca caatatcggc aactgataaa
      661 tgagtttctg ccaaaggccc taatacatct atatcccaag ctgtattttc ttccgttgaa
      721 caagattgga aaagtatacc tgttccaatg agtagaaatg aaaggaaacg aaaacgcgaa
      781 tgtttaatca tgcggtgtaa aaatagccaa aaaactgggc tgtagggcac gaaaaaaggc
      841 agcctaagct gccttttgta tctcgtttta ttctgtaatt aagatattac tgaatattga
      901 tggtgtatgt atttccgttt atggtaactg ttacctgatt gtcgcaatca ccagatccaa
      961 aatctaccgt acgttgcgga cgattagatg gtgttaccac taaagttcca ctaactgttt
     1021 ggtgacagct acgtttatgc accaatggcg atgtaatctg tgaagtccaa gtatcgccgc
     1081 taacttttgt tccattcgaa gaaccggtca cactaaatac atcatctgaa aaaagaagtg
     1141 gtgtattgaa tccttcagtc catgtacgaa cacgagaagc tgtccaagtg attgttcctc
     1201 cattattagc tagtacaata ctaccatcaa cttgaactgt ataagttggc tgaccttgag
     1261 cattattacc tgtattcgtt actgttcgtg tacctaaaat ctgattgtta ttcacgaaat
     1321 agttgagtgg agtgattgtg ataactgttc cttgatctcg gtagcgacct gtagtgctga
     1381 tgatgatttt acctgaacga gtttttaaat cattccctaa gcaacttgtt ccaaagtcga
     1441 tgataattgt atcgggattg gttgctgaaa cagttccgga agtatcgaaa gtaattgtag
     1501 cacaattgga taggagaaca ccttcgtcgg ctgcaagctt gaatgaagat aaatcgcctg
     1561 ttttagcagc ttgatctgaa atgtcattta gttcgttgaa gtaacgttca gcaacacttt
     1621 cgtttttaga agcattggta tcgctatcgt tatctgaatc ttttctacat gaactgaaag
     1681 aaatcagcaa tgtagctgca gccagagcag aggcgcgcaa agaaaaagga attgctttca
     1741 tagtttttgg gtttttatag ctacctgacg gaagtattcg aataaggttt aaacgaaaat
     1801 acttcaggaa gattttagta ttccgaaaac tgcaggtaat ttattaatag ctggtttgtt
     1861 ttgtttccag cggctaaccg gaatggtccg aatccaacca tcttgaagtg ttagattaac
     1921 gccaatacaa agtcgcgtag ctggatttaa aacttgaaaa caatcttcga gtaatgctgt
     1981 gtttcgatag ggagtttcga taaagaactg agcataaccg gtttgaaacg aaatttgttc
     2041 taattccctt agttttcggg tacgtgcaat tttatcaatg ggtaaataac catgaaacgt
     2101 aaattgttgt ccgtttaatc ccgagcccat gatactgagc atgatagccg aagggccagg
     2161 aataggaaca accggaattc ccatatcatg cgctaatgca agaagactgg ctccaggatc
     2221 agcaatggca ggagctccag cttcagaaag aacacctgca tctgtaccat ttcgcacaag
     2281 tgttagtagt tgaagttgtt cttctatacg agtgtctttg tcaacgcgat gtaaggtgat
     2341 atcatcgaaa gaacctgtaa atcctgcttt tcgtaggaaa cggcgagcac ttttttcgtt
     2401 ttcggtgaag taaactttta gctcttgtgg ctgggaaaca ggtttattac aagagctaaa
     2461 agtttacttc
//
LOCUS       DAOOQW010000294         2460 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_52017_length_2460_cov_4.702703, whole genome shotgun sequence.
ACCESSION   DAOOQW010000294 DAOOQW010000000
VERSION     DAOOQW010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52017_length_2460_cov_4.702703"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..496)
                     /locus_tag="PK281_11525"
     CDS             complement(<1..496)
                     /locus_tag="PK281_11525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY50018.1"
                     /translation="MKVRYFSLLILFIFLSCSKEKRSDKAFGNMQDFVIHISNYAHEL
                     NSDFIIIPQNGIELAFNDQDPANGLNLAYLNAIDGVGVEELFYNGVYALDAERLSMLQ
                     QVKQSKKVLVSEYVNDDAFISDAYIQNEVESFICFTRTSQNFDYIQIPDTIPNENNLN
                     IMQLS"
     gene            complement(556..>2460)
                     /locus_tag="PK281_11530"
     CDS             complement(556..>2460)
                     /locus_tag="PK281_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOY50019.1"
                     /translation="TALQLLPQDYAFPYYRDDSMLLGIGMQPYDLMLQLMAKLDDPFS
                     GGRTYYCHPSLRDADKPKIPHQSSATGMQAIPATGTAMGFWYKENVALNDPQITDLPL
                     VVCSLGDASVTEGEVAEAFQMAALKQLPILYLVQDNGWDISANAKETRAQSAYDYIKG
                     FNGIEAISIDGANFSECYEALSKVIHTIRSERRPFLVHAKVPLLNHHTSGVRMEWYRD
                     DLEEARSRDPYPVLIQQLLEAGFTQSELDVLEQSAKAKVQADYDRAIQAEDPRPEDLF
                     THDFAPTPITEESGNRTPQRDDKVLMVDCALFAVEELMRKHPECLLYGQDVGARLGGV
                     FREAATLAQKFGDERVFNTPIQEAFIVGSTVGMSAVGLKPIVEVQFADYIWPGLNQLF
                     TEVARSCYLTNGKWPVSMILRVPIGAYGSGGPYHSSSVESILANIRGIKIVYPSNGAD
                     LKGLMKAAYYDPNPVVILEHKGLYWSKVKGTDEARTIEPAEDYILPLGKAAIVQEIEE
                     QKTRETISVITYGMGVHWALNASKGMKDKVEIIDLRTIYPLDEKAIFASVRKTKKCLV
                     VTEESVSNSFARSLSGLIQEQCFESLDGPVCVIGSENLPAIPLNSTLEQTMIPNIDKV
                     KKAMEDLLRY"
BASE COUNT          760 a          537 c          450 g          713 t
ORIGIN      
        1 gagaaagctg cataatgttt aaattattct cattgggaat ggtatccgga atttgaatat
       61 agtcgaagtt ctgactggtt cgagtaaagc aaataaatga ttctacttca ttttgaatat
      121 atgcatccga aataaatgca tcatcattaa catattcaga aaccagaacc tttttactct
      181 gttttacttg ctgaagcata gataaacgct ccgcatctaa agcataaact ccattataaa
      241 acaattcctc cactcctact ccatcaatag cgtttaaata cgctaagttt agcccatttg
      301 caggatcttg atcattaaat gctaattcaa ttccattctg aggtatgatg ataaaatcgg
      361 aattcaattc atgcgcataa ttcgaaatat gaatgacaaa atcctgcata tttccaaatg
      421 ccttatccga acgcttttcc ttggaacaag agagaaaaat gaaaagaata agtagtgaaa
      481 aatagcggac tttcatttaa acagaatcgg aataatttaa gtgaaaaccg attgaatttg
      541 cgctacaaac taaaatcaat aacgcaataa atcctccatt gctttcttca ctttatcaat
      601 attcggaatc atggtttgct ctaaagtgct attgagcgga attgccggta aattctctga
      661 accaatcaca caaacaggtc catcgagtga ttcaaaacac tgttcttgaa ttaatcctga
      721 aaggcttcta gcaaaggaat tactgactga ttcttctgta acaactaagc atttctttgt
      781 cttacgaaca gaagcaaaaa tggctttttc atccaaagga taaatggtgc gcaaatcaat
      841 gatttcgact ttatccttca tccctttgga agcattcaac gcccaatgca cgcccattcc
      901 ataagtaata acggatatag tttcacgtgt tttttgttct tcaatttcct gaactatggc
      961 tgcttttccg agcggtaaaa tataatcctc agctggttca atggttcgcg cttcatcggt
     1021 gccttttacc ttagaccaat acaagccttt gtgttccaaa atcaccaccg gattcggatc
     1081 gtaataagca gctttcatca atcctttcaa atccgcaccg ttgcttggat aaacgatttt
     1141 aattcctcga atgttagcta aaatactttc tactgaagat gagtgatatg gaccaccact
     1201 tccataggcg ccaattggaa cacgcaaaat catgctcacc ggccattttc cgttagtcaa
     1261 gtagcaagaa cgtgccactt cggtaaaaag ttgattcaaa ccaggccaga tataatcggc
     1321 gaattgtact tctacaatag gtttcaatcc aactgctgac attcccaccg ttgaaccaac
     1381 aataaaggct tcttgaatag gcgtattgaa tacacgttcg tcgccaaatt tctgagcaag
     1441 tgtggcagct tcgcggaata caccacctaa acgagctcct acatcttgtc cgtataataa
     1501 gcactcagga tgtttgcgca tgagttcttc cacagcaaac aatgcacaat caaccatcaa
     1561 cactttatcg tctcgttgag gcgttcgatt tccactttcc tcggtaatag gcgtgggtgc
     1621 aaaatcatgg gtaaataaat cttctggacg cggatcttct gcctgaatag cacgatcgta
     1681 atcggcttgt actttcgcct tggcagattg ctccaataca tctaattcag attgtgtaaa
     1741 tccagcttct aataattgtt gaataagcac tggataagga tcacgggaac gagcttcttc
     1801 taaatcgtcg cgataccatt ccatccgcac acccgatgta tggtgattga gcaagggcac
     1861 ttttgcatgc actaagaaag gacgacgttc gctacgaatg gtgtgaatga ccttcgataa
     1921 agcctcataa cattcactga aattagctcc atcaatagag atagcttcaa ttccattaaa
     1981 tcctttgata taatcgtacg cactttgagc tcgggtttct ttggcattag cagaaatatc
     2041 ccaaccattg tcttgtacta aatacaaaat aggtaactgc ttcaaagccg ccatttgaaa
     2101 ggcttccgct acttcgcctt cggtaacgga tgcatctcct aaagaacaaa ccactaaggg
     2161 taaatcggtg atttggggat cgtttaatgc gacattttct ttgtaccaaa atcccatggc
     2221 agttcccgtt gcaggaatag cttgcatccc agttgcggat gattgatgcg gaattttagg
     2281 tttatcggca tcgcgcaaac tggggtgaca gtaataagtt ctaccacctg aaaaaggatc
     2341 atccagtttg gccattaatt gcaacatcaa atcataaggc tgcatgccaa ttccaagcaa
     2401 catagaatcg tcgcggtaat aaggaaatgc atagtcttga ggaagcaatt gcaaggccgt
//
LOCUS       DAOOQW010000295         2441 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_52625_length_2441_cov_5.388097, whole genome shotgun sequence.
ACCESSION   DAOOQW010000295 DAOOQW010000000
VERSION     DAOOQW010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52625_length_2441_cov_5.388097"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="PK281_11535"
     CDS             complement(<1..309)
                     /locus_tag="PK281_11535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY50020.1"
                     /translation="MRVLLILFIWLTLIPVELDAQSPAIDSLLNKIRLSRNESEKLDL
                     VLALCRRNDTHLDTFNRYAFIARDLAAHSNDPVKVAYAQYNVAYAYYFSNANDSARLV
                     I"
     gene            455..1543
                     /locus_tag="PK281_11540"
     CDS             455..1543
                     /locus_tag="PK281_11540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY50021.1"
                     /translation="MKKIGLLILSFSLSLFSASAQSILRYTPSNGSGEVYTNLDSAYA
                     DAQNDDILYLSGNTFQFSSNAVEKRLQWVGAGVNSDSTIATGYTRINPINSSTISFII
                     RPNAGGSSFTGINFGGFRLNDCDVAGNINLVTFSRCMFMTDWISLSYNSNYAGSQLNY
                     RFQECIFNTFVNGNGFSSNATSNITFDNCLFSKGLFRLRTGTYAFNNCVFWATLDSDP
                     ISQNSGCTFNSCIFWDGANANLLGGYMNESNVFDHCVFSHATIPVGGSGANPTIVNSC
                     IANVTNLFVDTNGNYNYEDGDDYRLVAGSEALTAAAAGGQCGIYGGASPAKPGNVPYN
                     PHVSSKNIAPATVNGLLNVNITTSAQSH"
     gene            1563..2300
                     /locus_tag="PK281_11545"
     CDS             1563..2300
                     /locus_tag="PK281_11545"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY50022.1"
                     /translation="MKAKSVMILFVALVASFGSFAQTMSAYEYWFDMDYANRTNVSIS
                     GGTTVNINASLNTESLSQGSHTLWFHTQSSDGRWSVPVSSPFVKGNNAIIGVEYWYDN
                     NYSTKEYMDLTPSQGEDYSLGLPVSGITIGNHQISISFVDNEQIRSVPISTSFYYDGS
                     VLGISDLKNMAGLHLFPNPANEEIQLSGLADYNHLVIYDIQGKVVLNQSTSGDTEKLK
                     IESLSPGLYTILLLSETQHTTLSFIKN"
BASE COUNT          759 a          448 c          487 g          747 t
ORIGIN      
        1 aatgaccaaa cgagcgctat cgttcgcatt ggagaaataa tacgcataag ctacattata
       61 ctgagcataa gcaactttca caggatcatt tgagtgtgct gctaaatcgc gtgcaataaa
      121 agcatagcgg ttaaaggtgt cgagatgcgt gtcgtttcgg cgacataaag ccaacactaa
      181 atcgagtttt tcggattcgt ttctacttaa tcggatttta ttcagtaggc tatcaatagc
      241 aggactttga gcatctaatt caactggaat tagcgtcaac caaataaaaa gaatcaatag
      301 aactctcatc accagttccg aaaattaacg agagataaat ctattggatt tcagggaatg
      361 gtttacatca gctaaatcac taatacaaat tgattattga tggagcattt tgaaagctgt
      421 caatttgtgt cattcattcc taacacatat tgtcatgaaa aaaatcggac ttttaattct
      481 aagcttttcg ttaagcttat tttcagcttc tgcccagagt atattaagat acacaccatc
      541 gaatggttcg ggagaagtgt atactaacct tgattcggcc tacgccgatg cacaaaacga
      601 cgacattctt tatttgtcgg gaaacacgtt tcagtttagt tccaatgcag tagaaaagcg
      661 cttacaatgg gttggagcag gtgtgaattc cgattcaact atcgcaactg gctacacgcg
      721 aatcaaccca attaattcaa gtacaatctc atttattatt cgcccaaatg caggaggttc
      781 ttcatttaca ggaatcaatt ttggtggatt tcgtttaaac gattgcgatg tagctgggaa
      841 catcaactta gtaacgttta gtcgatgcat gtttatgacc gattggataa gtcttagtta
      901 caactcgaat tacgctggat ctcaactcaa ttaccgtttt caagaatgta tcttcaatac
      961 gtttgttaat ggaaatggat tttcatctaa tgcaacttct aacataacct ttgataattg
     1021 tctattctcc aaaggattat ttagattacg tactggaaca tacgccttca acaactgtgt
     1081 tttctgggca actctagata gtgatcctat atctcaaaac tcaggatgta cttttaatag
     1141 ttgcattttt tgggatggag cgaatgctaa tcttcttggg ggatacatga atgaaagtaa
     1201 tgtgttcgac cattgcgttt tctcccatgc aaccattcca gttggtggtt caggtgctaa
     1261 ccctacaatt gtcaactcgt gtattgccaa cgtaaccaac ctttttgttg atactaacgg
     1321 aaattataac tacgaagatg gcgacgacta tcgattagtt gctggtagcg aagcattaac
     1381 ggcagcagcg gcaggtgggc aatgtggtat ctatggtggt gctagtccag ctaaaccggg
     1441 aaatgtgcct tacaatccac atgtgagcag caaaaacatt gctccagcaa ctgtaaatgg
     1501 tttactcaat gtaaacatca ccacatcggc tcaaagtcat taatcgctta acactagaaa
     1561 ttatgaaagc gaaatcagta atgatcctat ttgttgcgct agtagccagc tttggaagtt
     1621 ttgctcaaac catgtctgca tatgaatatt ggtttgatat ggattatgcc aatcgcacga
     1681 atgtgagtat ttcgggtgga accacggtaa atatcaatgc atcattaaat accgaatcgt
     1741 taagccaagg aagtcacacg ttgtggtttc acacgcaaag cagcgatgga agatggtcgg
     1801 tgccagttag cagtccgttt gtgaaaggca acaatgcaat tataggtgtt gaatattggt
     1861 acgataacaa ctacagtaca aaagagtaca tggatttaac acctagtcaa ggagaagatt
     1921 attccttggg tttaccagtg agtgggatta ctatcggtaa ccatcagatt tcaataagtt
     1981 ttgtagataa tgagcaaata cgaagcgttc cgattagtac atcattttac tacgatggta
     2041 gtgttttagg aatttcagat ttaaaaaata tggctggatt acacctattt cccaatcccg
     2101 cgaatgaaga aattcagtta agcggattag cagattataa ccatttagtg atttatgaca
     2161 ttcaaggaaa agtagtttta aatcaatcta catctggaga tactgagaag ctaaaaatcg
     2221 aaagtttatc tcctggttta tacaccattt tactgctcag tgaaactcag cacacgaccc
     2281 tgagttttat caaaaactaa gagcgttttt gagagaatgg tgttgatgct caggttagag
     2341 ttccgttaat ctggaattct tcctgagcat tctaatttta taagaattgt ggctaattgt
     2401 tttcgaatta tgctagccta tttttatttt ccaacaacaa c
//
LOCUS       DAOOQW010000296         2426 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_53087_length_2426_cov_3.808098, whole genome shotgun sequence.
ACCESSION   DAOOQW010000296 DAOOQW010000000
VERSION     DAOOQW010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53087_length_2426_cov_3.808098"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1272
                     /locus_tag="PK281_11550"
     CDS             <1..1272
                     /locus_tag="PK281_11550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY50023.1"
                     /translation="VFGEDKKSQSNSIAATKPINAETDTFKVGRQRLYQTAYYPDFLV
                     TQLDRGFLNQTYQPYTGTGYQNPSINGLFRIGLADLFEDFRITGGVRLAANLTGNEYL
                     LAFQTFKKRLDKTILFHRQGLQSNPAEGARVMMHTISGKLSYPFSEIARVEGTLSYRN
                     DRNVYLATDLSNLSRANTYNHWAQLKTEFVLDNSFPVGLNMMTGTRLKITGEHYHRLD
                     QAKTSVSILGADFRQYGRITREMIWAVRLAGATSFGPNKLLFYLGGVDSWFIPRFDNS
                     VSVDETQKYIFQTLGTNVRGFYQNIRNGSSFAVFNGEVRWNLIRYAFKYPLKSELLNN
                     LQVIGFTDVGTAFTGSSPYSDKNTFNQKTIESGPIKVVLKNQREPIVAGFGFGVRTKI
                     LGYFVRLDMARGLEDGTLLPALFHLSFTTDF"
     gene            1275..1661
                     /locus_tag="PK281_11555"
     CDS             1275..1661
                     /locus_tag="PK281_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOY50024.1"
                     /translation="MTKKTPMDLTIVLLLLLIGLIAGFASGLVGIGGGIVIVPALVYF
                     LSLSQHNAQGISIGMLLMPVGFLAAINYYKAGQFNMKYSLIIGATFVVGAWLGSKVSL
                     SIDQQMMKRIFGVFVLLMALKMIFGK"
     gene            1658..>2426
                     /locus_tag="PK281_11560"
     CDS             1658..>2426
                     /locus_tag="PK281_11560"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HOY50025.1"
                     /translation="MSISPEQLKLLVKEEAQQLFPLIRTIRRHLHQHPELSFEEFETS
                     KYLQQILQNHALSFTNGWAGTGIRAVLTGNGQGKTLALRGDMDALPIQETSQTEYTSQ
                     HTGVMHACGHDVHSACVLGASIILQKLREHWAGEVLVVFQPGEEKLPGGARLMLEAGV
                     FEDKVPSSVMAQHVFPSLPAGKVGFRAGTYMASTDEIYITVRGKGGHGAMPQQVNDPI
                     LASAQIILALQQVTSRLALPGVPTVLTIGKVIAEGATN"
BASE COUNT          692 a          452 c          552 g          730 t
ORIGIN      
        1 gtgtttggag aagacaagaa atctcaatcg aattctattg cagccaccaa accgattaac
       61 gccgaaacgg atacgttcaa agttggtcgt cagcgattat atcaaaccgc ttattatccc
      121 gatttcttag ttacacagtt agatcgaggc tttctcaatc aaacctatca gccatatacg
      181 ggaacaggat atcagaatcc atccatcaat ggattattcc gaataggatt agctgattta
      241 tttgaggatt ttcgaattac tggaggcgtt cgtttagctg ccaatttaac tggaaacgag
      301 tatttactgg catttcaaac gtttaagaaa cggctcgaca aaaccatact ttttcatcgc
      361 caaggcttgc agagtaaccc agctgaagga gcgcgagtga tgatgcatac gattagtggc
      421 aaactatcgt accccttttc tgaaatagct cgagtggaag gaactttatc gtatcgtaac
      481 gatcggaatg tttatctagc aactgattta tcgaatttat ctcgcgctaa tacgtataat
      541 cattgggcgc aattaaagac cgaatttgta ttggataatt cctttccggt tggattaaat
      601 atgatgacag gcacacgctt gaaaatcacg ggagagcatt atcatcggct cgatcaggca
      661 aaaacctcgg taagtatttt gggggcagat tttcgtcaat atggacgcat cactcgcgaa
      721 atgatttggg cggttcgttt agctggtgcc acttcgtttg gaccgaataa gctgttattt
      781 tatttaggcg gagttgattc ttggtttatt ccacgatttg acaattctgt ttcggttgat
      841 gaaacccaga agtacatttt ccaaacgttg ggaaccaatg ttcggggatt ttaccaaaat
      901 attcggaatg gttcaagctt cgccgtgttt aatggagaag taaggtggaa tttgattcgc
      961 tatgcgttta aatatccact caaatcggag ttgttaaaca acttgcaagt aattggtttt
     1021 acggatgttg gaacagcttt tacgggatct tctccttact ccgacaagaa tactttcaac
     1081 caaaaaacga ttgaaagtgg tcctattaaa gtagttttga aaaatcaacg cgaaccaatt
     1141 gttgctggat ttggttttgg ggtgagaaca aaaattcttg gttactttgt aagacttgat
     1201 atggctagag gtctagagga tggcacgctg ctaccagctt tgtttcattt aagtttcaca
     1261 acagattttt aaacatgaca aaaaaaactc ctatggattt aacaattgta ttgttgctgc
     1321 ttttgattgg tttaattgca ggattcgcta gcggattggt gggaattgga ggagggatag
     1381 taatcgtacc tgcacttgtt tattttctta gtttgagtca acataatgca cagggaattt
     1441 ccataggaat gctactgatg cccgttgggt ttttagctgc catcaattat tacaaagctg
     1501 ggcaattcaa catgaaatat agcttaatca taggcgccac attcgttgtt ggagcttggt
     1561 tagggtcgaa agtttctttg tcgattgatc aacaaatgat gaagcgaatt ttcggtgtat
     1621 ttgtgcttct gatggctttg aaaatgattt tcggtaaatg agtatcagtc ccgaacaact
     1681 taaactattg gtgaaagaag aagcgcaaca gctttttcca ctcattcgta ctattcgccg
     1741 tcatctgcac caacatcctg aattatcatt cgaggaattc gaaactagca aatacctgca
     1801 acaaatactg caaaatcatg cattgtcgtt taccaatggt tgggcaggaa ctggtattcg
     1861 agcagttttg actggaaacg gacagggaaa gacgctcgct ttaagaggcg atatggatgc
     1921 tttacccatt caagaaactt ctcaaacaga atacacatcc caacacactg gtgtaatgca
     1981 tgcttgcgga catgatgtgc attcggcttg tgtgttgggc gctagtataa ttcttcaaaa
     2041 gctacgtgaa cactgggctg gagaagtttt agtggtgttt caaccgggtg aggaaaaact
     2101 tcccggagga gcgcgattaa tgctcgaagc tggagtgttc gaagacaaag ttccatcatc
     2161 agtaatggcg caacatgttt ttccgtcttt acctgccgga aaggttggtt ttcgtgccgg
     2221 tacctatatg gcttcaactg atgagattta cattacagta agaggcaaag gtggacatgg
     2281 agcgatgcct caacaagtga acgatcccat tttagcttct gcacaaataa ttttggcctt
     2341 gcaacaggtg actagtcgtt tggctttacc gggcgttcct actgttttaa cgatagggaa
     2401 agtaatagcc gaaggtgcta ccaatg
//
LOCUS       DAOOQW010000297         2401 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_53856_length_2401_cov_4.791986, whole genome shotgun sequence.
ACCESSION   DAOOQW010000297 DAOOQW010000000
VERSION     DAOOQW010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53856_length_2401_cov_4.791986"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..973)
                     /locus_tag="PK281_11565"
     CDS             complement(<1..973)
                     /locus_tag="PK281_11565"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HOY50026.1"
                     /translation="MTYIRPKFRHPELPKNDLGYSVDYYEGSLSTLCAGCGHDSISAG
                     IIQAAYEMNIEPHRLAKLSGIGCSSKTPAYFLSNSHGFNSVHGRMPSVATGANMANRD
                     LIYLGVSGDGDSASIGMGQFVHVIRRNLNMVYIVMNNGCYGLTKGQDSATADIGSKNK
                     SGNTNLFQSIDLASLAIELGATFVAQSFSGDKSQLVPLIKAAMKHPGFAFINVISPCV
                     TFNNNVGSTKSYDFVREHVEATATIDFVPEMSEITTSYNEGTMKAIELHDGTYLQLHK
                     LNQEWDPSDRFSAITAVQKAKVNNEILTGLLYLNPDAVDLHEMISTSS"
     gene            complement(998..>2401)
                     /locus_tag="PK281_11570"
     CDS             complement(998..>2401)
                     /locus_tag="PK281_11570"
                     /inference="COORDINATES: protein motif:HMM:NF013974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOY50027.1"
                     /translation="RQRQLFKNIIYVGALSALLNIDFSVFESLLEEQFKGKEKLIAPN
                     IKALKMGVDYIHANHAFPLDIRVERRDLVGDSILIDGNSACGLGAVYGGATVAAWYPI
                     TPSTSVVEAFMNYSDEFRVDPITGKRNVAIIQAEDELSAIGMVIGANWNGARSFTATS
                     GPGLSLMNEFLGLAYFAEIPTVLIDVQRTGPSTGMPTRTQQSDILEGAYASHGDTKHI
                     MLFPSTPKECFDMTVDAFDLTEQLQTPVILMTDLDLGMNDHMSKPFQWDDHREYKRGK
                     VLDAEALEDIERFGRYLDVDGDGIAYRTYPGTHPTKGSYFTRGTSRDEYAVYTENGDV
                     YEKNMDRLIKKWNTAKDYVPAPDLYQDSNTSKYGVLFFGTSQYSTEEAIDILKADGVN
                     LDAIRIKSFPFNKTVEDFIHSHDKVFVVEQNRDAQMKSLLMIELAANPQKLVSVLNYN
                     GLPITADYIVKSIQAAI"
BASE COUNT          725 a          504 c          469 g          703 t
ORIGIN      
        1 ttgaagaagt gcttatcatt tcatgcaaat caacagcatc cggatttaaa tataaaagcc
       61 ccgtaaggat ttcgttattc acttttgctt tttgcaccgc tgtaatggcc gaaaaacgat
      121 cagaaggatc ccattcttga ttgagtttat gcagctgcaa ataggttcca tcgtgcaact
      181 caattgcttt catggtgcct tcgttataag aagtagtaat ctcagacatt tcaggaacaa
      241 aatcgattgt ggctgttgct tccacatgtt cgcgtacgaa gtcgtatgat ttagtagaac
      301 ccacgttatt attgaatgtc acacaaggtg aaatcacatt aataaatgca aatcctggat
      361 gtttcattgc agctttaatg agcggaacaa gctgtgattt atcacccgaa aaactttgag
      421 caacaaacgt agcacctagt tcaatggcca aactggctaa atcaatcgac tgaaataaat
      481 tggtatttcc tgatttattt ttcgaaccaa tatcagctgt agcagaatct tgtcctttcg
      541 ttagtccata acaaccgtta ttcatcacga tatacaccat gttgagattt cggcggatga
      601 catgaacaaa ctgtcccatt ccgattgatg cagaatcgcc atcacccgaa acacctaaat
      661 aaataaggtc acgatttgcc atgttagctc ccgtagcaac cgaaggcata cgtccgtgta
      721 cactgttgaa accgtgcgaa ttacttagaa aataagcagg cgttttagat gaacaaccga
      781 taccagaaag cttagccaaa cgatgcggtt cgatattcat ttcgtatgca gcttgaataa
      841 tacctgcact gatagaatcg tgtccacagc ctgcacataa agttgaaagt gaaccttcgt
      901 aataatcgac cgaataacct aaatcgtttt taggaagttc ggggtgacgg aatttcgggc
      961 gaatgtatgt catggtgatg ttgcctgatt ggaaaaatta aatagcagcc tgaattgatt
     1021 taacaatata atcggctgta attggtagtc cgttgtaatt gagtaccgac actaattttt
     1081 gaggattagc agccagttct atcatgagca aacttttcat ttgcgcatca cgattttgct
     1141 ctacaacaaa tactttatcg tgagagtgaa taaagtcttc taccgtttta ttgaatggga
     1201 atgattttat tcgaatagca tctagattca caccatctgc ttttaaaata tcaatcgcct
     1261 cttcggttga atactgggat gttccaaaga ataaaactcc gtatttcgaa gtattggaat
     1321 cttgatatag atctggagcc ggcacataat ctttggcggt attccatttc ttaatcaatc
     1381 gatccatatt tttctcatac acatcaccat tttcggtata tacagcatat tcgtcacgtg
     1441 aagttccacg cgtgaaatag gatcctttgg ttggatgtgt tcccggatac gtgcgataag
     1501 cgataccatc accatctaca tcgaggtaac ggccaaaacg ctcaatatct tctagtgctt
     1561 ctgcatccaa tacttttcca cgtttgtatt cccgatgatc gtcccattga aatggttttg
     1621 acatgtgatc attcatcccc aaatcaagat cggtcataag aatgacagga gtttgcagct
     1681 gctcagttaa gtcgaacgca tctaccgtca tatcaaaaca ctctttcgga gtagaaggaa
     1741 atagcatgat atgttttgta tcaccatgcg aagcataagc gccttccaaa atatcggatt
     1801 gctgtgtacg agtaggcatt ccagtagaag gaccagttcg ttgcacatca attaacacgg
     1861 taggaatttc agcaaaataa gctagtccga ggaattcatt catgagcgaa agccctgggc
     1921 cgctggtggc tgtaaatgaa cgtgctccat tccaattagc gccaatcacc attccgattg
     1981 cagaaagctc atcttcggct tgaatgatgg ctacatttcg ttttccggtg atgggatcaa
     2041 ctcggaattc atcagaataa ttcatgaaag cttctaccac cgaagtggaa ggagtaattg
     2101 ggtaccatgc agctacagtt gcgccaccat aaacagcacc taaaccacaa gctgaatttc
     2161 catcaattaa aatagaatca ccaactaaat cgcggcgctc aacccgaata tccagtggaa
     2221 atgcatgatt tgcatgaata taatcaacac ccattttcaa tgctttgatg ttgggtgcga
     2281 tgagtttttc tttgcctttg aattgctctt ccaacaagct ttcgaaaaca ctgaaatcga
     2341 tattaagcaa ggctgataat gcacctacgt aaataatgtt tttgaataat tgtcgctgac
     2401 g
//
LOCUS       DAOOQW010000298         2394 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_54103_length_2394_cov_4.764429, whole genome shotgun sequence.
ACCESSION   DAOOQW010000298 DAOOQW010000000
VERSION     DAOOQW010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54103_length_2394_cov_4.764429"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="PK281_11575"
     CDS             <1..606
                     /locus_tag="PK281_11575"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HOY50028.1"
                     /translation="ASGGYYIAAPCDTIVASPNTITGSIGVFGLFFNARQMVNNIGVT
                     IDTVKTNPYADIMTASRAVTAQEAEVIQKEIERIYDNFVTRVSEGRHMTKANVDSIAQ
                     GRVWSGVDAKRLGLVDVFGGLDKAIEIAAKKAGLTSYRIQELPYQEDPFQKFMKQLGG
                     DSEAAILQKTLGDSYRYYQQLEHLRAMKSYQARLPYELELY"
     gene            682..2352
                     /locus_tag="PK281_11580"
     CDS             682..2352
                     /locus_tag="PK281_11580"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOY50029.1"
                     /translation="MTNKEISRALKLIASLGELHDENPFKLKSFTNAAFNVDRAGDAL
                     EGKTLEELIQIPGIGKSIAQVILDIIHTGTTTDLAHYASITPAGVIEMLGIKGIGPKK
                     VATIWKELGIESPGELLYACHENRLVELKGFGAKTQESIKIALEYRLANQGKYHYAEA
                     ETVANELIRLIHEQDDSLQIAFTGELRRFMEVIENIEVLFVSNVHELELILQQIPSLQ
                     IESVLEDSIVVKFAEKYSMYLICCEEDQYEREQLLRTGPPEHLEELDLDPEEPVESET
                     SFYEERGFPFILPELRDLPVDNAASIDIEKLVVWKDIRGILHNHSTYSDGANTLEEMA
                     IACRDLGMEYLGICDHSKSAFYAGGLSIEKVIQQHKEIDQLNLKLGPFKIFKGIESDI
                     LSDGSLDYPNEILASFDLVVASVHSILNMDVDRATSRLITAIENPYTTILGHPTGRLL
                     LSRKGYPIDHKKVIDACAANGVIMELNAHPYRLDIDWRWIPYCIEKGVQISINPDAHR
                     KEGYTDMQYGINVARKAGLTHDSLFNSKSLPEMQSFLSSKVNTQHRNS"
BASE COUNT          764 a          473 c          461 g          696 t
ORIGIN      
        1 gcttcgggcg gttattacat tgctgctcct tgtgatacta ttgttgctag cccgaatacg
       61 attactggtt caattggtgt atttggttta ttcttcaatg ctcgccaaat ggtgaataat
      121 attggtgtta ctatcgatac agtaaaaacc aatccgtatg ctgatatcat gactgcatct
      181 cgcgccgtta cagctcaaga agccgaagta atccaaaaag aaattgagcg tatttatgac
      241 aatttcgtta cccgtgtttc tgaaggccgt cacatgacta aagcgaatgt agattctatt
      301 gctcaaggtc gcgtttggtc tggtgtagat gcaaaacgtt taggtttagt tgacgttttt
      361 ggaggattag acaaagccat cgagattgca gctaaaaaag caggtttaac ttcataccgt
      421 attcaagaat taccttatca ggaagatcca tttcaaaaat tcatgaagca attaggtggt
      481 gattcagaag ctgcgattct tcaaaaaaca ttaggtgatt catatcgcta ctatcagcaa
      541 ttagaacact tacgtgcgat gaaatcctat caagcacgat taccatacga gttagaactt
      601 tactaattcc taattcttaa attgaaacgc tagtgataac ttcggttttc attagcgttt
      661 ctgttttaac cattcaccaa aatgaccaat aaagaaattt cccgtgcttt aaagttgatt
      721 gcctccttag gtgaattaca cgacgaaaat ccgttcaagc taaaatcatt taccaatgca
      781 gcttttaatg tagatcgggc cggtgatgct ttggaaggaa agaccttgga agaattaatt
      841 caaattccag gaattggtaa aagcatcgct caggttattt tagatattat acacactgga
      901 actactaccg atctcgccca ttatgcttct ataacaccag ccggagttat tgaaatgctt
      961 ggaattaaag gaattgggcc aaaaaaagtg gctactatct ggaaagaact aggcattgaa
     1021 tctcccggag aattactgta tgcctgtcat gaaaaccgtt tagttgagct aaaaggattt
     1081 ggtgccaaaa ctcaagaatc catcaagata gcacttgaat atcgtttggc aaatcagggt
     1141 aaatatcatt atgccgaagc cgaaacagtt gctaatgagc tcattcgatt aatacacgaa
     1201 caagacgatt cacttcaaat tgcattcact ggagaactcc gcagattcat ggaagtaatt
     1261 gaaaacatcg aagtattatt tgtctcaaat gttcatgaac ttgaattgat tcttcaacag
     1321 attccaagtt tgcaaattga gtcagtactg gaagatagta tcgtcgtcaa attcgctgaa
     1381 aagtattcca tgtacttaat ctgttgcgaa gaagatcaat acgagagaga gcagctttta
     1441 cgaaccggtc cacctgaaca tttagaagaa ctcgatttag atcccgaaga acccgttgaa
     1501 tcggaaacta gcttttatga agaacgggga tttcctttta ttcttcctga attgcgcgat
     1561 ttacctgttg ataatgctgc atcaattgat atagaaaaac tggttgtttg gaaagatatt
     1621 cgaggcattt tacacaatca cagtacatac agcgacggag ccaatacatt agaagagatg
     1681 gcaattgctt gtcgcgattt agggatggaa tacctcggca tttgtgacca cagcaaatcg
     1741 gcattttatg cgggtggatt aagcatcgaa aaagtaattc agcaacataa agagatagat
     1801 caactgaatc taaaattagg tcctttcaag attttcaaag gaatcgaaag cgacatccta
     1861 agcgatggtt cgctcgatta tccaaatgaa attcttgcct catttgattt agttgttgct
     1921 tctgtccatt ccattttaaa tatggatgtt gatcgagcaa caagccgact aattacagct
     1981 attgaaaacc catacaccac catccttggt catccaacag gtagactatt actttcgcgt
     2041 aaaggctatc caatcgacca taaaaaagta attgatgcct gtgccgcaaa tggagtaatt
     2101 atggaattaa atgcccatcc gtatcgctta gatattgact ggcgttggat accctactgc
     2161 attgaaaaag gagttcaaat ctccatcaat cccgacgccc atcgaaaaga aggatatacc
     2221 gatatgcaat atggcatcaa cgtcgcccga aaagccggac tcacacacga ttccctcttc
     2281 aattcaaaat ccctccccga aatgcaatcc ttcctttcat cgaaagttaa tacccaacat
     2341 cgtaattcgt aattcgtaat tcgtaattcg taattcgtaa ttcgtaattc gtaa
//
LOCUS       DAOOQW010000299         2386 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_54354_length_2386_cov_4.271557, whole genome shotgun sequence.
ACCESSION   DAOOQW010000299 DAOOQW010000000
VERSION     DAOOQW010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54354_length_2386_cov_4.271557"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="PK281_11585"
     CDS             <1..560
                     /locus_tag="PK281_11585"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HOY50030.1"
                     /translation="KHAVITSVDRDDLKDGGSIIWVETIQAIRQASPGTTLETLIPDF
                     MGKWENLQRIIDIRPEIVSHNLETVRRLTKQVRIQAQFDRSLEVLRRLKEGGCNTKSG
                     VMLGLGETETEVLETIDELAQNKVDILTLGQYLQPTPKHLPVIDFITPEKFAEYREYA
                     LTKGFRYVESGPLVRSSYHAEKHLF"
     gene            723..1766
                     /gene="gap"
                     /locus_tag="PK281_11590"
     CDS             723..1766
                     /gene="gap"
                     /locus_tag="PK281_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOY50031.1"
                     /translation="MSKIRVAINGFGRIGRISFRAILSKSNIEVVAINDLTNVHTLAH
                     LLKYDSVHGRLPVAVNATENSIEAGGHNMRVFAEKDPANLPWKELNVDVVIESTGFFL
                     DKESAGKHLTAGAKRVIISAPAKGDVETVVLGVNDQILAKNEAILSNASCTTNCAAPM
                     VKVLDDAFGVEAGLLTTIHAYTGDQHLVDAPHNDLRRSRSAALSIIPTSTGAAKAIAL
                     ILPHLKGKLDGLAMRVPIPAGSMTDLTVKLKKEASVAEINAAFKKAADESMKGILEYT
                     EDPIVSVDIVGNPHSCIIDSGLTMVMGQTVKVVGWYDNETGYSNRIADLIEKMAALPT
                     FSQNGLNQEPVTA"
     gene            1881..>2386
                     /locus_tag="PK281_11595"
     CDS             1881..>2386
                     /locus_tag="PK281_11595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY50032.1"
                     /translation="MKTLSSVEKLDVANIGLMLISCLLAFILPFELFLFSYAVLGPLH
                     YLTEIGWLHKKGYYTTGKYDFVFLGILSIFITIGFIGLNDSLKDLATGLVYFGFLSAL
                     MMVLIKDNTLKIISIILILFSSILINKYTFFETFFAIYLPTIIHVFFFTGLFILYGAL
                     KNKSWLGIL"
BASE COUNT          733 a          447 c          487 g          719 t
ORIGIN      
        1 ttaaacatgc cgttattact tcggtcgatc gggatgattt aaaagacgga ggttccatca
       61 tttgggtcga aacgattcaa gctattcgtc aagcaagccc tggtacaacg ctcgaaacac
      121 tcatccccga tttcatgggg aaatgggaga atctgcaacg tattatcgac atccgaccag
      181 aaatcgtttc tcataattta gaaaccgttc gccgactaac caaacaagtt cgcattcagg
      241 ctcaatttga tcgaagctta gaagtacttc gtcgcttaaa agaaggtggt tgcaacacta
      301 aatcgggcgt tatgcttggc ttaggcgaaa ccgaaacgga agtgctagaa acaatcgatg
      361 aactagcgca gaacaaagtc gatattctca ccttgggaca gtatcttcaa ccaactccca
      421 aacatcttcc ggtgattgat tttatcacac ccgaaaaatt cgccgaatac cgcgaatatg
      481 cgttaacaaa aggattcagg tatgtagaaa gtggaccatt ggttcgttcc tcgtatcatg
      541 ctgaaaaaca tttgttttag ttgctgaatt agcaaaaccg aaacaaaagc gatactgtac
      601 ccaacgaatc cgaaataaat cggaagtgtt aataattatt taaccgcaat tagggataat
      661 tatttgcata agtagttttc aaagtgtaat tttgacactc aataagattt aaaaccatca
      721 acatgagtaa aatcagagta gctataaatg gattcggtcg aattggacga attagttttc
      781 gggctatctt atctaaatcg aatatagaag ttgttgcgat aaatgattta acgaacgtac
      841 atacacttgc tcatttgcta aagtatgatt cggttcatgg acgcttacct gttgcggtga
      901 atgcgactga gaattctatc gaagcaggtg gacataacat gcgcgttttt gctgaaaaag
      961 atccggcaaa ccttccatgg aaagagttga atgtggatgt tgtgattgaa tcgacaggtt
     1021 tctttttgga taaggaatcg gctggaaaac acttgacagc aggagctaaa cgtgtaatta
     1081 tttctgcacc tgcaaaaggc gatgtggaaa cggttgtttt aggtgtgaat gaccaaattc
     1141 tggctaaaaa cgaggctata ctttcgaatg catcttgcac gacaaattgt gctgcaccaa
     1201 tggttaaagt actggatgat gcatttggtg tagaagcagg attgcttact accattcacg
     1261 catacacagg agatcaacat ttggttgatg cgcctcacaa cgatttacgt cgttcccgtt
     1321 cggcggcatt gtcaatcatt ccaacaagta caggtgctgc aaaagcaatt gctcttattc
     1381 ttcctcattt aaaaggaaag ttagatggat tggccatgcg cgttcctatt cctgctggat
     1441 cgatgaccga tttgacagta aaattgaaaa aagaagctag tgttgcagaa atcaatgctg
     1501 catttaagaa agctgcagat gaaagcatga aaggtatttt ggaatacacc gaagacccaa
     1561 ttgtttctgt agatatagtt ggaaatccac attcgtgtat catcgattcg ggcctcacta
     1621 tggtgatggg acaaacggtg aaagtggtag gatggtacga caatgaaacc ggttattcga
     1681 accggattgc tgatctgata gagaaaatgg ctgcattgcc aactttttca cagaacggcc
     1741 tgaaccaaga acctgtaact gcataatata ggttagtttg attttgggaa acggagatcc
     1801 cggtagcttt gctaaccggg attttctatt ttatatcaaa gctgtactaa aaattaagct
     1861 ttctactaac ttcgtgcatc atgaaaacac tatcctccgt tgaaaaatta gatgtggcca
     1921 acattggact catgctaatt tcttgcctac tcgcatttat actaccgttt gaattatttc
     1981 ttttttctta cgccgtttta ggtccacttc actacttaac tgaaataggt tggctccata
     2041 aaaaaggata ttatacaact ggtaaatacg attttgtgtt tcttggaatt ctcagcatat
     2101 tcatcacaat tggttttatt ggattaaatg attcgttaaa agacttagct acagggcttg
     2161 tatattttgg gttcctttcg gctttaatga tggtgctcat taaagacaat acactcaaaa
     2221 ttataagcat catactgata ttattttcgt caatcctgat aaataaatac accttttttg
     2281 agacgttttt cgccatctat cttcctacta tcattcatgt ctttttcttt acaggcttat
     2341 tcattttata cggtgcattg aaaaataaaa gttggttagg aatcct
//
LOCUS       DAOOQW010000300         2376 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_54673_length_2376_cov_3.961224, whole genome shotgun sequence.
ACCESSION   DAOOQW010000300 DAOOQW010000000
VERSION     DAOOQW010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54673_length_2376_cov_3.961224"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(288..1985)
                     /gene="ggt"
                     /locus_tag="PK281_11600"
     CDS             complement(288..1985)
                     /gene="ggt"
                     /locus_tag="PK281_11600"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002681098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HOY50033.1"
                     /translation="MINRIIPLLLVGLVVACQSVPTKNTERHVGLITNSAMVVSAHPL
                     ASKAGADVMRKGGNAVDAAIATQLALAVVHPSAGNIGGGGFMVIRQKDGSLNTLDYRE
                     KAPSAATSTMYLNEQGDVIPGLSESGQLSAGIPGTVAGLVESHARYGKLSWKELVQPA
                     IDLALYGFLLTQKEANGLNYIQEDLKKYNSILPQFLINEKWEAGDSIRYIDLGHALER
                     IRDRGLAGFYEGKTADDIVAEMQRGNGLITHEDLKNYKPVWRAPIVASYKEYKIISMP
                     PPSSGGIALAQLMRSVENYPIKDWGHNNTKTVHLMVEAERRAYADRSVYLGDPDFYDV
                     PVQQLTDKKYIDDRMNTFDAKTATPSKAIREGVFILKESDQTTHLSIVDSEGNAVSIT
                     TTLNDSYGSKVVVAGSGFILNDEMDDFSVKPGVPNMYGAVGGEANKIEAAKRMLSSMT
                     PTIVEKNGKLFMVVGTPGGTTIITSVFQTILNVVEFKMTMQEAVSAKRFHSQWLPDEV
                     FPENGTMDSVTIKKLESMGHTLQFRGEIGRVDAILVTPDGKLEGGADPRGDDAAAGF"
     gene            2027..>2376
                     /locus_tag="PK281_11605"
     CDS             2027..>2376
                     /locus_tag="PK281_11605"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOY50034.1"
                     /translation="MDWETLSCFTYTKQPLYSMSLRKEKAARLKIHVLDHTLKLIGKK
                     SFEDLYVDDICEKVKISKVTLFKYFPQKEDILLYYFRMWCFSRSVDLNQKPKEGMQGI
                     YYLFDKASEECELHP"
BASE COUNT          673 a          547 c          438 g          718 t
ORIGIN      
        1 gatataaagg aaatcatcat caaactctac cttaaaaact tttaagtgag catctatcca
       61 tttataaatt aagaagccca gaatgtgaaa aagtatactg ttgagaataa aatactgtcc
      121 ttgctgctgc gtagtgaacc aagcataatg gcaataaaca aacaggttat gatgaaaata
      181 aaaccatacc gatagaactc cagataacga gtcgataaat ttcttacacg cgatttttgt
      241 atcatctccg aaaacggttc ttagttaaac tattacttca agtaaaatta gaacccggct
      301 gcagcatcat cacctcttgg atctgcacct ccttctaatt ttccatcagg agtaacaagt
      361 atggcatcta ctcgtccgat ctcacctctg aattgtagcg tatgacccat gctctctaat
      421 ttctttatgg tgacgctatc catcgttccg ttttccggaa acacttcatc gggcaaccac
      481 tgactgtgaa accgtttggc tgacactgct tcctgcatcg tcattttaaa ttcaacaacg
      541 tttaaaatag tttggaatac tgaagtaatg atggttgttc caccaggtgt acctaccacc
      601 ataaagagtt taccattctt ctctacaatg gttggtgtca ttgaactcag catccgttta
      661 gcggcctcaa ttttattggc ctctcctcct actgcgccat acatattcgg cacaccaggc
      721 ttcacactaa agtcatccat ttcatcattc aaaataaaac ccgatcctgc caccactact
      781 ttcgatccgt aactatcatt tagtgttgtc gtaatcgaaa cggcattgcc ttctgaatct
      841 acaattgata agtgagtggt ttgatccgat tcctttagta tgaacacacc ttctcgtatt
      901 gctttgcttg gtgttgctgt ctttgcatcg aatgtattca tgcgatcatc aatgtatttt
      961 ttgtcggtga gttgctgtac cggcacatca taaaaatcgg gatcacctaa ataaacggat
     1021 cgatcagcat atgctctgcg ttccgcttca accatgaggt gaactgtctt ggtgttgtta
     1081 tgtccccagt cttttatcgg ataattttct actgaccgca taagctgcgc caatgcaata
     1141 cctccgctcg atggtggtgg catcgaaatg attttgtatt ccttgtatga ggcaacaatt
     1201 ggagcacgcc aaacgggctt gtagttttta agatcttcat gcgttatcaa tccatttcca
     1261 cgctgcattt cggcaacaat atcatcagct gttttcccct cgtaaaatcc ggcaaggcca
     1321 cgatcgcgaa tgcgctccag tgcatgacct aagtcaatgt aacgaatgct atcgccagcc
     1381 tcccactttt cgttaatcaa aaactgaggc aatatggaat tgtatttttt cagatcttcc
     1441 tgaatgtagt tcaatccatt cgcttccttt tgggtaagga gaaaaccata taaagcaaga
     1501 tcaatcgcag gctgaacaag ttctttccag gacaattttc cataacgcgc atgcgattca
     1561 accaaaccag ctacggttcc tggaatacct gcagaaagtt gtccgctttc actcaagcct
     1621 ggtatcacat ctccttgctc atttaaatac atggtacttg ttgccgctga tggagctttc
     1681 tcccgatagt ccaatgtatt gagtgaacca tctttttgtc tgatcaccat aaagccacct
     1741 ccacctatat tacctgctga gggatgcact actgctaatg cgagttgggt cgcaatggct
     1801 gcatcaactg cattgccacc tttgcgcatc acatcggctc cggctttcga agcaagtgga
     1861 tgagctgaga ccaccatagc tgaattcgta atcagaccaa catgtctctc cgtattcttt
     1921 gttggaaccg actggcatgc gactactaat ccgacaagta aaagtggaat tatacgattt
     1981 atcatttggc ttttggttaa gtccctataa aagtattaat tccatcatgg attgggaaac
     2041 cctctcctgt tttacctata ctaaacaacc cctctactca atgagcctgc gaaaagaaaa
     2101 ggctgcacga cttaaaatcc atgtgcttga tcatacctta aaacttatcg gcaaaaaatc
     2161 gtttgaagat ttgtatgtag atgatatttg tgagaaggta aaaatatcga aagttacctt
     2221 gttcaaatac tttcctcaaa aggaagatat cttactgtat tactttagaa tgtggtgctt
     2281 ctcgcgatcg gttgatctga atcaaaagcc aaaggaaggt atgcagggaa tttattactt
     2341 atttgataaa gccagcgaag aatgtgaact ccatcc
//
LOCUS       DAOOQW010000301         2341 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_55849_length_2341_cov_3.797900, whole genome shotgun sequence.
ACCESSION   DAOOQW010000301 DAOOQW010000000
VERSION     DAOOQW010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55849_length_2341_cov_3.797900"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /locus_tag="PK281_11610"
     CDS             complement(<1..472)
                     /locus_tag="PK281_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014032673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family peptidase"
                     /protein_id="HOY50035.1"
                     /translation="MEKPVDSRRKQFIRKLRHKYRLVVMNDETFEEKYSLKLSQLNLF
                     TALGLLSIVLIILTTFVIAFTPLREYIPGYSSDVRMRRNLLVLAEKVDSLKKSTEDKD
                     ALLDNIQRVMNGSVQTGPTKPRQSTKPDMNAVKLTASEKETQFREQVEEQDRYNL"
     gene            599..>2341
                     /locus_tag="PK281_11615"
     CDS             599..>2341
                     /locus_tag="PK281_11615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOY50036.1"
                     /translation="MQKLNYIRPLLYALLVLLITGGLIGCSTKKNKWLNRTFHNITSK
                     YNGYFNGEETIKEAVATLRTGHVDNYIKVLPVYDLGSLEDSKTIIPLTDKAIKKASVV
                     ISRHSMMIKGKEYCKYIDDSYLLIGKSMFYKRDYYASLEMFAYVAREPVKNNKKDPIQ
                     HRANIWLCRAYSELGMFTDARLAIDRSLNDKTLPKNIKGELYTALTDFHLKQNHYAEG
                     LESITEALKYVKKKKDRVRLLFIRGQLLQKAGEIKQASDSYSKVLKSNPTYEMSFYAR
                     INIARCFDSENKKSTEIRALLAKMLADPKNIDYKDQIYYVLGELDEKEGNETRAIEQY
                     TTSVQASTTNVNQKGMSYLAIAEIYFDQREYRIAAAYYDSTISSLQKDFPDYKIIENK
                     RNSLAELVKMYEKIELYDSLLSMAKLNPAELDKKAEQIFKKEEEARRKQKEKDAILAA
                     KLAASGGNTSAAPGIAGNGSWYFYNTSTMSFGYTEFRKVWGDRKLEDNWRRSNKQTVA
                     PISGNPDEDTTGSDVAKEEIKLTREDSIALIRKKLEKSVPKNDTMLRAYADSVMEGYY
                     DLGLIYKERLADLKK"
BASE COUNT          782 a          432 c          464 g          663 t
ORIGIN      
        1 tcaagttgta gcggtcttgt tcttcaacct gttcccgaaa ctgagtttct ttttctgacg
       61 ctgtaagttt tacggcattc atatcgggtt ttgtgctttg tcgtggctta gttggacccg
      121 tttgcacact cccattcatt actcgttgaa tattatccaa taaggcatct ttatcttccg
      181 ttgatttttt taatgaatca actttttcgg ccaagactaa caaatttctt cgcatacgaa
      241 cgtcgcttga atatccggga atgtattcgc gaagaggcgt aaatgcaatg acaaaagtgg
      301 ttaatatgat taatacaatt gacaacaaac ccagagcagt aaagagattg agctgagata
      361 atttgaggga atatttctcc tcaaacgtct catcattcat tactaccaaa cggtatttat
      421 gacgtaattt ccggatgaat tgcttccggc ggctatcaac aggtttttcc atgaccgctg
      481 ctaagttctg aaattcactc cggattgttt gaaattgaat ggattcatcc ttaaaatagc
      541 ggaaattaaa ataattttgc gctccaacag ttatttagcc gcaacgtata tttttcattt
      601 gcaaaagctc aactacatac gcccactgct ttatgctctt ttagttctcc ttataacagg
      661 cggattgata gggtgttcca ctaagaagaa taagtggctt aaccgaacat ttcataatat
      721 cacctctaaa tacaatggct attttaatgg agaagaaacc ataaaagagg ctgtcgctac
      781 acttcgaact ggacatgttg ataattatat taaggttctt cctgtgtatg atcttggttc
      841 attagaagat tccaaaacca ttattccctt aacagataaa gcaattaaga aagcatcggt
      901 ggttattagt cgacactcga tgatgattaa aggcaaagag tactgtaaat acatcgacga
      961 tagttacttg cttatcggaa aatcgatgtt ttacaaacgc gactactacg catcattaga
     1021 aatgtttgct tatgtagctc gcgaaccagt aaaaaacaac aaaaaagatc ctattcaaca
     1081 ccgcgcgaac atttggcttt gtcgtgccta tagtgaattg ggaatgttta cagatgctcg
     1141 tttagccata gatcgatcac tcaacgataa aactcttccc aaaaacatca agggagaatt
     1201 gtataccgca ttaaccgatt tccatctgaa gcaaaatcat tatgctgaag gcttggagtc
     1261 gattaccgag gcattgaaat atgtgaaaaa gaagaaagat cgggttagat tgctctttat
     1321 tcgtggacaa ttgctacaaa aagcaggaga aataaaacaa gcatccgata gctattcaaa
     1381 ggttctaaaa agcaatccca cgtatgagat gtctttttat gcacgaatta acatagctcg
     1441 atgcttcgat tcagagaata agaaaagcac agaaatacgg gcattgttgg caaaaatgct
     1501 tgctgatcca aaaaacatcg attacaagga tcagatttat tatgtgttgg gagaattgga
     1561 tgaaaaagag ggtaacgaaa cccgggctat cgagcaatac accacatctg ttcaagcaag
     1621 taccacgaat gtgaatcaaa aaggaatgtc ctatttggcg attgctgaga tttattttga
     1681 ccaacgtgaa tatcgaattg ctgcagctta ttacgatagt acaatcagtt cattacaaaa
     1741 ggactttccc gattataaaa tcatcgagaa caagcggaac agcttagccg aattggttaa
     1801 aatgtatgag aagattgagc tttacgacag tttacttagt atggcaaaat tgaatcctgc
     1861 agaattagat aaaaaggcag agcagatttt caagaaagaa gaagaagcac gtaggaaaca
     1921 aaaagagaaa gatgcaatct tagcagccaa attagctgca tctggtggca atacttctgc
     1981 tgctccagga attgccggta atggaagttg gtatttctac aatacttcca ccatgagttt
     2041 tggttataca gaattccgca aagtttgggg cgatcggaaa ttggaagaca actggagacg
     2101 ttctaataaa caaacagtag ctccaatttc aggcaatccc gatgaagaca caaccggttc
     2161 tgatgttgca aaagaagaaa tcaagctaac acgtgaagat agtattgcat taatccgcaa
     2221 aaaactagaa aagtccgttc ctaaaaacga caccatgttg cgtgcttatg ccgattcggt
     2281 aatggaaggc tattacgacc ttggattgat ttacaaagaa cgattagctg atttaaaaaa
     2341 a
//
LOCUS       DAOOQW010000302         2312 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_56874_length_2312_cov_4.828533, whole genome shotgun sequence.
ACCESSION   DAOOQW010000302 DAOOQW010000000
VERSION     DAOOQW010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56874_length_2312_cov_4.828533"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /locus_tag="PK281_11620"
     CDS             complement(<1..578)
                     /locus_tag="PK281_11620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY50037.1"
                     /translation="MPKLLAIAFFLIGISGLVKAQSGATKLVFSPTYNGKSIQPGKAI
                     YVSELQDSVSLDDFRFYISEVKFISPDGSIFPFSKHHFLCDFQHPESLQIACPPIISN
                     ESESVLWFQIGIDSTTSSSGAFGQDLDPVHGMYWAWNSGYINVKIQGQSPKCPARNHQ
                     FEYHLGGYLVPNNAITSIQRRIIPGKDIQIEI"
     gene            818..1159
                     /locus_tag="PK281_11625"
     CDS             818..1159
                     /locus_tag="PK281_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018944298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOY50038.1"
                     /translation="MNIENTKAQMRKGVLEFCILSVLSNGDAYTSDIINRLKESKLIV
                     VEGTLYPLLTRLKNTGLLSYRWEESTSGPPRKYYGLTENGQRVLSELRISWDELVASV
                     AQTQISSPSKS"
     gene            1163..>2312
                     /locus_tag="PK281_11630"
     CDS             1163..>2312
                     /locus_tag="PK281_11630"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HOY50039.1"
                     /translation="MNKTISINLSGMLFNLEEAAYERLHRYLQQIRKSFEGTDGQDEI
                     VGDIESRIAELFAERLKNKQVILEQEVIDVIAIMGEPETYKDEEEAPTSGSNPGNQNY
                     DAYQSRRLFRDPDDAVLGGVCSGLGYYFGLDPVWLRLAFVIALFFAGSGPLLYIILWI
                     VLPKANSTAEKLQMRGEQVNIENIERRIKEEAARFRERAEAFGEEARQGFKNANVHGR
                     LGSFMEEFSAVFLGFFRRIILFMGRSFGVFLIFLGGVLLFAFLSTVFNSGNLISINDE
                     DGISSFSVQAFFGTFFSSPDQQKLFLVGLSLVILAPIIGLLLGGLRLVLHPRLRMGWF
                     GPVNGMLFLAGLVCCIVAGAMLVGEFSAKGRMIENVALSTPVGDTLELA"
BASE COUNT          669 a          465 c          506 g          672 t
ORIGIN      
        1 tcgatttcta tttgaatgtc tttcccagga ataattctgc gctgaataga cgtaatcgca
       61 ttgttgggaa ctaaatatcc acctaaatga tattcaaatt gatggtttcg agccggacat
      121 ttaggacttt gaccttgaat ttttacgttg atgtaaccac tattccaagc ccaatacatg
      181 ccatgaactg ggtcgagatc ttgtccgaaa gcaccggaag aactagtggt gctatcaatt
      241 ccgatttgaa accaaagcac ggattctgat tcgttcgaaa taattggagg acaagctatt
      301 tggagcgatt cgggatgttg aaaatcgcag aggaagtgat gcttcgaaaa gggaaaaatg
      361 gatccatctg gcgaaatgaa ttttacttcg gatatataga atcgaaaatc gtcaaggcta
      421 acactgtcct gcagttcgga aacataaatt gcttttccag gctgaataga tttcccattg
      481 tatgtgggac tgaaaaccaa cttcgttgct ccagattgag ccttcaccaa tccagaaata
      541 ccaatcagaa agaaagcaat agcaaggagt ttaggcatcc ccgaaaatta acattttaga
      601 attgaatatc gaaacttcaa taaataagga atcttaaaca agagtcgatt ttacctgaat
      661 ggtttagccg atttgaaaat agaaattgaa ggaggatctc gcaggattca aaaatattat
      721 aaatttattg agctgtattt caaatactta aataaataat ccatccagta ccttgcatcg
      781 catagtaccg tgttaagata agttctatgt ttgcactatg aatatcgaaa ataccaaggc
      841 gcagatgcga aaaggagtgc tggagttctg catactctcg gttttgtcga atggtgatgc
      901 ctacaccagc gacatcatca accgcctcaa agaatctaaa ctcattgtgg ttgaaggaac
      961 tttatatccg ctgctaaccc gacttaaaaa taccggcttg ctcagttacc gttgggaaga
     1021 aagcacttcc ggtccgcctc gaaaatatta cggattaacc gaaaatggac agcgtgtttt
     1081 gtccgaactc cgcatcagct gggacgagct ggtagcatct gtagctcaaa cccaaatttc
     1141 ttcaccttcg aaatcctgac ccatgaataa gactattagc attaacctca gcggcatgtt
     1201 gtttaacctc gaagaagctg cttacgagcg acttcatcgt tacttacaac aaatccgcaa
     1261 atcgttcgaa ggaaccgacg ggcaagatga aattgtgggt gatattgaaa gtcggatagc
     1321 tgaattattc gccgaacgac ttaaaaataa gcaggtcatc ctcgaacagg aagtgataga
     1381 tgtgattgct atcatgggtg aacccgaaac ctataaggat gaagaagaag ctcctacatc
     1441 gggctctaat ccaggaaatc agaactacga tgcgtatcag tcgcgccgct tatttcgcga
     1501 tccggacgat gccgtattag gtggtgtgtg ttcaggatta ggatattatt ttggacttga
     1561 tccagtttgg ttgcgtttag cttttgtgat agccttgttc tttgcaggtt caggtcctct
     1621 actctacatc atcctctgga ttgttttacc aaaagccaat agcaccgccg agaaacttca
     1681 aatgcgcggt gagcaagtca acatcgaaaa catcgaacgt cgcataaaag aagaagctgc
     1741 tcgctttcgt gaacgcgccg aagcctttgg tgaagaagcc cgtcaaggtt ttaaaaatgc
     1801 taatgttcac ggtcgtttag gtagcttcat ggaagaattt tcagccgtat ttctaggctt
     1861 cttccgaaga ataatcctat tcatgggacg atcatttgga gttttcctca ttttcctagg
     1921 tggtgtgcta ctcttcgctt ttttgagtac cgttttcaat tcaggaaacc tcatatctat
     1981 caatgatgaa gatggtattt caagcttttc agttcaggct ttctttggaa ctttcttctc
     2041 ctctcccgat caacaaaaac tgtttctggt aggactttca ctcgtcattt tagctccaat
     2101 tatcggtttg cttttaggtg gattgcgatt ggtccttcat ccacgtttac gcatgggttg
     2161 gtttggacca gtgaatggaa tgctctttct agcaggttta gtttgttgca ttgtagctgg
     2221 agctatgttg gtgggcgagt ttagtgcaaa aggacgcatg atagaaaatg tggctctatc
     2281 aacaccagta ggcgatacct tagagttggc tg
//
LOCUS       DAOOQW010000303         2300 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_57363_length_2300_cov_3.239198, whole genome shotgun sequence.
ACCESSION   DAOOQW010000303 DAOOQW010000000
VERSION     DAOOQW010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57363_length_2300_cov_3.239198"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="PK281_11635"
     CDS             <1..598
                     /locus_tag="PK281_11635"
                     /inference="COORDINATES: protein motif:HMM:NF015587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HOY50040.1"
                     /translation="SFGAIFALFYATNGMNSIIESFNASIFIKESRPDWKIRLVSLAL
                     TIILSLLLVLAFTLMVVTNYGLEYLINHHLITYGINYVLIWLGKWVIFYGLLFFSVAF
                     LYYLGPSKNARAGFFSIGALATSFLILLLLIGFSFFVGNFGTYNTVYGSVGALIAVMV
                     LINLNAMLLLAGYEFNAGVYHLHRKRKGRISNEEANLY"
     gene            633..>2300
                     /locus_tag="PK281_11640"
     CDS             633..>2300
                     /locus_tag="PK281_11640"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HOY50041.1"
                     /translation="MRALLLRILFSALAPLALSAQQLPYDPIHPPSSYRNSDNPQYWG
                     NRMHFPGYWQQDVEYSIKAIVDEKTDIVTATEELKYYNNSPDTLTFVYFHLYQNAFQP
                     GSYCSALHEANDYPIVYGKYEKDKLGTVVSNLQVNGQVVKTELENTILKVFLPYPLMP
                     NSAVVFSMDFKTYFDYQGSIRRRMKEFGSYGSKHYDGVHWYPRMAVYDRKFGWCTDQH
                     LGKEFYGDFGSYDVELEFSDNYVMEATGYLLNRDEVLPADLRQKLDIKNFANKPWEEA
                     PSVITPYNPAKRKTWKFHAENVHDFAWTADPNYRIGEAEWNGIQMVALVQEPHAARWQ
                     NAADYTAKIIETYSRDFGMYVYNKMVVADARDGMEYPMLTLDGGADPNYRGLLAHEVG
                     HNWFFGMVGNNETYRASLDEGFTQFLTVWAQEHIDGPYALKGKFANKYTAKYTKPEEV
                     RYARALYGYMRDAIREDETTLNTHSDDFQGAVGHGGGYGNVYYKTATMLFNLQYLLGD
                     TLFSNAMKNYFNEWKIAHPYFEDFRNSIINYTHSDLNWFFDQWLETFK"
BASE COUNT          660 a          467 c          462 g          711 t
ORIGIN      
        1 ttcatttgga gccatcttcg cattgttcta tgctaccaat gggatgaatt caattattga
       61 atcattcaat gcaagtattt tcattaaaga atcccgtccc gactggaaaa tccgactagt
      121 ctctttagct ttaactatca tcctttccct tttgctcgtt ttggcattta cgctcatggt
      181 tgtgacgaat tatgggctcg aatacctcat caatcatcac ttaataacat acgggattaa
      241 ctatgtattg atttggttag ggaagtgggt gattttctat ggcttgctct ttttctcggt
      301 tgcttttctc tattatctcg gtccatctaa aaatgcaaga gctggatttt tctccattgg
      361 agcattagca acaagttttc tgatcctcct gctattgatc ggattttcat tctttgtcgg
      421 taatttcgga acctataata ctgtttacgg ttctgtagga gcattgatcg cagtcatggt
      481 gctcattaac ctaaatgcca tgctattatt agctggatat gaattcaatg caggtgttta
      541 tcatcttcat aggaaaagaa aaggacgtat ttctaacgaa gaagctaatc tttactaagc
      601 attcactatt tttcgcggta aattctattt gcatgcgagc acttctactg cgtatccttt
      661 ttagcgctct tgcgccattg gctctttctg ctcaacaact tccttacgat ccaattcatc
      721 caccaagttc ttatcggaat tctgataatc ctcaatactg gggaaatcgc atgcattttc
      781 ccggctattg gcaacaagat gttgaatata gtattaaagc tatagtagac gaaaaaacag
      841 atatcgttac tgcaactgaa gagttaaaat actacaataa ctcgccagat acattgacgt
      901 ttgtgtactt tcacttgtac caaaatgcct ttcaacccgg ctcatattgt tcggcactcc
      961 acgaagccaa cgactacccg attgtttatg ggaaatacga aaaagataaa ctgggcacag
     1021 ttgtaagcaa cttgcaagtg aacgggcagg tagtaaaaac agaactcgag aacactattc
     1081 tgaaagtttt cctcccatat cctttgatgc cgaattcagc agttgtgttt agcatggatt
     1141 tcaaaacgta tttcgattat caaggttcaa ttcgccgtcg aatgaaagag tttggttcat
     1201 acggctctaa gcattacgat ggtgtgcatt ggtatcctcg aatggctgtg tatgaccgca
     1261 aatttggttg gtgtaccgat caacatttag ggaaggaatt ctatggtgat tttggttctt
     1321 acgatgtaga actcgagttt tccgataact acgtgatgga agcaacaggt tacttgctga
     1381 accgtgatga agtattacca gccgatttac gtcagaaact cgacatcaaa aatttcgcaa
     1441 acaaaccttg ggaagaagct ccttctgtca ttactcctta taatccagct aaacggaaaa
     1501 cttggaagtt ccacgccgaa aatgtgcacg actttgcttg gacagctgat cctaattatc
     1561 gcattggtga agccgaatgg aatggaattc aaatggttgc acttgttcaa gaaccacatg
     1621 ctgctcgttg gcaaaatgcc gcagactaca ctgctaaaat catcgaaacc tacagccgtg
     1681 attttggaat gtatgtatac aataaaatgg ttgtggctga tgcacgcgat ggtatggaat
     1741 atccaatgct aaccctcgat ggaggtgctg atccaaacta tcgcggttta ttagctcacg
     1801 aagttggaca taattggttt tttggaatgg tggggaataa cgaaacctac cgcgcttctc
     1861 tggatgaagg ttttacccaa tttttaacgg tttgggctca agaacatatc gatggtcctt
     1921 atgcgctcaa aggtaaattc gcgaataagt acacagctaa atacaccaaa cccgaagaag
     1981 tacgctatgc tcgtgcatta tatggataca tgcgggatgc aattcgcgaa gatgaaacaa
     2041 ccttaaacac gcattcggat gattttcagg gtgcggttgg tcatggtggc ggatatggaa
     2101 acgtatatta taaaacggct accatgctgt ttaatttaca atacctatta ggtgacacct
     2161 tgttttccaa tgcaatgaag aactatttca acgaatggaa aatagcccat ccgtattttg
     2221 aagatttccg caattcaatc atcaactata cacattccga tctcaactgg ttctttgatc
     2281 aatggctcga aacttttaaa
//
LOCUS       DAOOQW010000304         2299 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_57405_length_2299_cov_3.277184, whole genome shotgun sequence.
ACCESSION   DAOOQW010000304 DAOOQW010000000
VERSION     DAOOQW010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57405_length_2299_cov_3.277184"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1875)
                     /gene="alaS"
                     /locus_tag="PK281_11645"
     CDS             complement(<1..>1875)
                     /gene="alaS"
                     /locus_tag="PK281_11645"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOY50042.1"
                     /translation="KKDAIAWAWELLTEVYKIDKSRLYVTVFEGDTSENLAFDQEAFD
                     TWKELIDPSRIINGNKKDNFWEMGDTGPCGPCSEIHVDIRSDEERARIDGKELVNASH
                     PQVIEIWNNVFMEFNRKADGSLEKLPAQHVDTGMGFERLCVVLQGKQSNYDTDVFQPL
                     ISFLENLSGKTYKNNEKDDIAMRVIADHIRTISFAIADGQLPSNNKAGYVIRRILRRA
                     VRYGYTFLGLKEPFMYTMVDTLADQMGEQFPEINSQRGLIKNVMHEEEQSFLRTLETG
                     INRFNDFVAKNTNHAQVPGDFAFELFDTYGFPIDLTELMAREQNLAVDMIGFNENLQK
                     QKARSRSAAVVEAGDWIELSEDDDEEFIGYDYLEAQVKITRYREVTAKGKKQFQLIFN
                     ITPFYAESGGQVGDSGILKDGNDIINIFDTKREHGVIVHFADKLPANLQASFKAEVHK
                     GKRISTMRNHTATHLMHAALRQVLGTHVEQKGSLVNDQNLRFDFSHFSKLSKEETRQI
                     EAIVNEQIRANILRDVQVLPIEEARKTGAMALFGEKYGDLVRVVTFDKSFSVELCGGT
                     HVPATGQIGYFRIVSESAVAAGVRRIEAITGEAAELQILEDANLVEQIKEVLKGAKDP
                     L"
     assembly_gap    1876..1885
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1886..2174)
                     /locus_tag="PK281_11650"
                     /pseudo
     CDS             complement(1886..2174)
                     /locus_tag="PK281_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023970.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
BASE COUNT          701 a          491 c          384 g          713 t
ORIGIN      
        1 taacggatct ttagctcctt taaggacttc tttgatttgt tctaccaagt ttgcatcctc
       61 tagaatctgc aattcagcag cttctccagt aatagcttcg attcttcgaa ctcctgcagc
      121 aactgcgctt tctgacacaa tcctaaaata accaatctgt ccagttgcag gtacatgggt
      181 tccgccgcat aattcaacag aaaacgactt atcaaatgtg actactcgaa ctaaatcacc
      241 atacttctct ccaaaaagag ccattgctcc tgttttacgt gcttcttcaa tgggtaaaac
      301 ttgaacatca cgaagaatat tcgcacgaat ttgttcattc acaattgctt caatctggcg
      361 agtttcttct ttactcaatt tagaaaagtg agaaaagtcg aaacgtaaat tctgatcatt
      421 taccaaagaa cctttttgtt ctacatgagt tcccaaaact tgacgaagag cagcatgcat
      481 caagtgagta gctgtatgat tacgcatcgt tgaaatgcgc tttcctttat gtacttcagc
      541 tttaaaactc gcttgtaaat tggcaggtag tttatcagca aaatgaacga ttactccatg
      601 ttcccgtttt gtatcaaaga tgtttattat gtcattccca tcttttaaaa tgcccgaatc
      661 gccaacctgt ccaccacttt cggcataaaa cggagtaatg ttaaatatga gttggaactg
      721 tttttttcct ttcgcagtaa cttctctgta tcgtgtaatt tttacttgag cttccaaata
      781 atcataaccg ataaattctt catcatcatc ttccgaaagt tcaatccaat cacctgcctc
      841 aactactgca gctgaacgtg aacgcgcctt ttgtttttgc agattctcgt taaagccaat
      901 catatcaaca gctaaattct gctcgcgagc catcaactcg gtcaagtcga tcgggaaacc
      961 atacgtatca aataactcaa atgcaaaatc acccggtact tgagcgtgat tagtattctt
     1021 tgctacaaag tcgttaaatc gattgattcc agtttctagt gtgcgtaaaa aagattgctc
     1081 ttcttcatgc atcacatttt taattaaacc tcgttgtgaa ttaatttcag gaaactgttc
     1141 gcccatctga tccgcaagcg tatctaccat ggtgtacata aatggctctt tcaatccaag
     1201 aaatgtataa ccataacgaa cagcacggcg cagaatccga cgaataacat atcccgcttt
     1261 attattgcta ggaagttgtc catcagcaat agcaaatgaa atggtacgaa tgtgatcggc
     1321 aataacgcgc atggcgatat catccttttc gttgttttta tatgtctttc cactaaggtt
     1381 ttcaaggaaa gaaataagtg gctggaaaac atccgtatcg taattcgatt gttttccttg
     1441 aagaacgacg cacaaacgtt cgaaacccat tccagtatca acgtgttgag caggcaattt
     1501 ttctaagcta ccatctgcct tccggttgaa ttccatgaag acattattcc atatttcaat
     1561 aacctgtgga tgactagcat tcacgagttc tttgccatca atacgtgctc gctcttcatc
     1621 acttcgaatg tcaacatgga tctcagaaca aggtccgcat ggtccagtat cgcccatttc
     1681 ccagaaatta tctttcttat ttccattgat aattcgagaa ggatcaataa gctctttcca
     1741 agtatcaaaa gcttcttgat caaaagctaa attctcggat gtatcacctt cgaaaacagt
     1801 aacatataat cgagatttat caattttata aacttcagta agaagctccc aagcccaagc
     1861 aatagcatct ttcttnnnnn nnnnnaagac caattgccta acatttcaaa catagtatgg
     1921 tgataagtat ccacaccaac ttcttctaaa tcattgtgct ttcctgaaac acgcaaacat
     1981 ttttgtgtgt ctgcaatcct tgggtataca ataggagaat ttcctaaaaa aagatcttta
     2041 aactgattca tcccagcatt tgtgaacatc agcgttggat cattcttaat gaccataggt
     2101 gctgaaggaa cgatttgatg cccttttgaa gcgaaaaaat cgaggaatgt ttgccggatt
     2161 tgcttggatt gcatactgaa ttaagaaaat ttatgatttc tgaaacggtg gcaaaggtag
     2221 taaaacagga tgcatatgat taaaaactga ttgacttagc ctacaataaa gattgtataa
     2281 atgaaaggat ttctaaatt
//
LOCUS       DAOOQW010000305         2283 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_57986_length_2283_cov_4.459156, whole genome shotgun sequence.
ACCESSION   DAOOQW010000305 DAOOQW010000000
VERSION     DAOOQW010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57986_length_2283_cov_4.459156"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..151)
                     /locus_tag="PK281_11655"
     CDS             complement(<1..151)
                     /locus_tag="PK281_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004917142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HOY50043.1"
                     /translation="MFTGIVESLGTVVKRKDEGNNTTFSLRCDFTSELQVDQSVAHNG
                     VCLTVT"
     gene            complement(153..>2283)
                     /locus_tag="PK281_11660"
     CDS             complement(153..>2283)
                     /locus_tag="PK281_11660"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOY50044.1"
                     /translation="NPGAFDPDGDSISYRMVDCRGEDGLIIPGFSQPQASSSFTLNAI
                     TGDLIWDAPLPNGVGEYNVAFVIEEWRHGILIGSVTRDMQISVVPCSNTPPQIEALPD
                     LCVDAGTILNFPVNATDSDSPIQSITLSATGGPFQFAPPGNAVFAPVTQMGFVSQQFI
                     WETSCAHVRKQPYSFSFKVIDNGNPNLSDFETMQIRVVAQAPENLQAVSAGSTIQLSW
                     EESPCNQAIGYAIYRKAGFYGYVHGPCETGVPAYTGYTFLTQVQGVTTLSYTDPGPLL
                     PGSSYCYMVIALFADGAESYASNEACVQLDKILPVITNVSVNTTASSSGELYIAWSKP
                     TAIDPVQFPGPFQYIIERAEGLSGTSFTLVDSLQSLDDTTYVDNNVLINTVGSAWRYR
                     ISLKNMSPDNVVEVGKSEAAASVFLSASGLDNQVNLSWQTNVPWQNDTFIVFRKLPTA
                     LQFDSLGYSITNQYSDLGLANGVTYCYRVKSKGKYSGAGFIDPILNWSQEVCAEAKDT
                     QAPCAPLAFISPNCDSLKNSITWEFPNDTSCFSDVVSIRVYFSTTSTGTWQLISEFPF
                     GTNQPFIHSPGDFVAGCYAVTAVDSFMNESELIRNCVDNCPVYELPNTFSPNNDGLND
                     AFQPFPWAFIAQVKMSIFNRWGQLVFETEDPDILWNGVNQTNNQKVPDGVYFYVCEVY
                     EDRLDGLVQRNLKGTIQVFGNSSKNGN"
BASE COUNT          693 a          504 c          455 g          631 t
ORIGIN      
        1 gagtaacagt taagcacaca ccattatgtg ctacactttg atccacctga agctccgatg
       61 taaaatcaca acgtaggctg aaagtcgtgt tattaccttc atccttgcgc ttgactacag
      121 ttcctaatga ttcaactata cctgtaaaca tcctagtttc cattttttga tgaattccca
      181 aaaacctgaa tggtcccttt taaattcctt tgaactaagc cgtccaaacg atcttcatac
      241 acctcacata cataaaagta tacaccatcg ggaaccttct gattgttcgt ttgatttact
      301 ccattccaga ggatgtcagg atcttccgtc tcaaaaacta actgtcccca acgattaaat
      361 atgctcattt tcacctgtgc aataaaagcc caaggaaaag gttggaatgc atcgtttagc
      421 ccatcgttat ttggagaaaa ggtgttaggt aattcgtaaa ccggacaatt atctacacaa
      481 tttcgaatca gctcactttc attcatgaat gaatcaacgg cagtaactgc ataacatccg
      541 gcaacaaaat ctccaggaga gtgaataaat ggctgattgg ttccaaacgg aaattctgaa
      601 ataagctgcc aagttccagt gcttgtggtg gagaaataaa ctcgaatact taccacatca
      661 gaaaaacaag aagtatcgtt cgggaattcc caggtaatgc tgttttttaa ggaatcacaa
      721 tttgggctga tgaatgctaa tggcgcacac ggagcttgcg tatcctttgc ttcggcgcat
      781 acttcttgtg accaatttag aatcggatcg ataaatcctg caccactgta ttttcctttc
      841 gatttcaccc gatagcaata agtaacgcca ttggctaagc ctaaatcgct gtattgattg
      901 gtaatactgt aacccaatga atcgaattga agggcagttg gtagttttct aaatacgatg
      961 aaagtatcgt tttgccaagg tacattcgtc tgccaagaaa ggtttacttg gttatcaagc
     1021 ccactggctg ataagaaaac agatgcggcc gcttcgcttt ttccaacttc caccacatta
     1081 tccggactca tatttttcaa cgaaatccta tatctccaag ctgaaccaac cgtattgatt
     1141 aacacgttgt tatccacgta tgttgtgtca tcaagcgatt gcaaggaatc aactagcgtg
     1201 aagctcgttc cacttaatcc ttctgcacgt tcaataatgt attgaaacgg tccgggaaac
     1261 tgaactggat caatagctgt aggtttggac caagcaatgt ataattcacc agaactactt
     1321 gcggttgtgt taacgctcac attggtaatt acaggaagga ttttatcgag ctgcacgcat
     1381 gcttcattcg aggcataact ttcggcacca tcagcaaata aggctatcac catataacaa
     1441 taagaagagc ctggaagcaa tggacctgga tcggtgtatg aaagtgtagt aacaccttga
     1501 acttgcgtta aaaatgtata ccccgtataa gctggaacac ccgtttcgca aggaccgtga
     1561 acgtatccat aaaatccagc tttgcggtaa atggcatatc caatggcttg gttacacgga
     1621 ctttcttccc aacttaattg aatcgtactt cctgccgata cagcttgtag gttttcgggt
     1681 gcttgtgcaa ccacgcgaat ttgcatggtt tcaaaatccg ataggttggg atttccatta
     1741 tcaatcacct taaagctgaa agaataaggt tgtttcctaa cgtgagcaca tgatgtttcc
     1801 caaatgaatt gctggctaac aaaacccatt tgagttacgg gagcaaaaac tgcatttcct
     1861 gggggggcga attggaaagg accacccgtc gcacttaatg tgatcgattg aattggactg
     1921 tcggaatcgg tagcatttac gggaaaattc aagatagttc cagcatctac acataaatca
     1981 ggtaatgctt caatttgagg tggagtattg gagcaaggaa caacactaat ttgcatatcg
     2041 cgagtaacgg aaccaatcag gataccgtgg cgccattctt cgatgacgaa tgccacgttg
     2101 tattcaccta cgccattggg caaaggagca tcccaaatca aatctccagt aatagcattg
     2161 agtgtaaaac tcgaactcgc ttgaggctga gaaaaacctg gaatgatcaa tccatcctca
     2221 cctcgacaat caaccattcg ataagaaatg ctatcgccat caggatcaaa tgctccagga
     2281 tta
//
LOCUS       DAOOQW010000306         2238 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_59711_length_2238_cov_4.795694, whole genome shotgun sequence.
ACCESSION   DAOOQW010000306 DAOOQW010000000
VERSION     DAOOQW010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59711_length_2238_cov_4.795694"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /gene="lepB"
                     /locus_tag="PK281_11665"
     CDS             <1..318
                     /gene="lepB"
                     /locus_tag="PK281_11665"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009388933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOY50045.1"
                     /translation="ILINGALATSYTFKQDYYFMMGDNRHNSADSRFWGFVPHDHIVG
                     KAVFIWLSRSTNRSFPNIRTERVFSFIRNDGVSSSYFLHIMIPLVLLILGWNNRNRFR
                     TQH"
     gene            complement(378..995)
                     /locus_tag="PK281_11670"
     CDS             complement(378..995)
                     /locus_tag="PK281_11670"
                     /inference="COORDINATES: protein motif:HMM:NF012697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine-cytidine kinase"
                     /protein_id="HOY50046.1"
                     /translation="MSRRPYVVGISGGSASGKTSFLKNLREALPEGAACIVSQDNYYL
                     PKEEQLKDENNQINFDLPTSIHRSAFHNDLCKLIRGEKVVLQEYTFNNASHSSQTIEL
                     TPAPIIIMEGLFIFHYEEIRNQLDLKVYIDAREDLKLARRLRRDKDERGYPEETVLYQ
                     WNNHVMPSYHQYLRPYRDDADIIVTNNISYDKGLLVLVNHLRSIC"
     gene            complement(1191..2060)
                     /locus_tag="PK281_11675"
     CDS             complement(1191..2060)
                     /locus_tag="PK281_11675"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOY50047.1"
                     /translation="MKSLDLTATILVPVDFSDITKPALAHAELYATIFKKPITLVHLI
                     DKGWGDKEHELKLEEEEALSKLQNFADDITNESGIEVSVLVRRGDFHEGIGELAEELN
                     ATLVVMGTKGIKGLQRWTGSHAMKVIKNTKDVPFIVVQDMPKRNDLKTVVLPFSFTVE
                     SRQKLAWAIHIAKMFQCHFLLLAEHQTDEFIQHKMQNNITFAKKYLDQHGCSYSVETA
                     SGSTAFHKEIIIYASAKDADLILIMTDEDREFTEYFTGTHEQDIIANERKLPVMVMNP
                     VDNMQILGAAMFQ"
BASE COUNT          647 a          445 c          417 g          729 t
ORIGIN      
        1 attctaatta atggtgcact tgcaacttca tacacattta agcaggatta ctatttcatg
       61 atgggtgata atcgtcataa ttcggccgat tctcgttttt ggggatttgt accacacgat
      121 cacattgtag ggaaagccgt atttatctgg ttaagtcgtt ccaccaatcg cagcttccca
      181 aatattcgta ccgagcgtgt atttagcttt attcgcaacg atggtgtgag tagttcgtat
      241 ttcttgcaca tcatgattcc actcgtatta ctcatcttag ggtggaacaa ccggaatcgt
      301 ttccgaacac agcattaaga ttgaattcaa aagaaaaatc cccgtcagtt tttaccggcg
      361 gggatttttt atttctctta acagatgctt cgtaagtgat tgactaatac gagcaatcct
      421 ttgtcgtagc tgatgttgtt ggttacaatt atatcagcat catcgcgata ggggcgaagg
      481 tattgatgat aacttggcat cacatggtta ttccattgat acaaaactgt ttcttcggga
      541 tagccacgtt catctttatc gcgacgtaaa cgacgagcta atttcaaatc ttctcgcgca
      601 tcaatataaa ccttcaaatc aagttgattc ctgatttcct cataatgaaa gatgaacaaa
      661 ccttccatga tgatgatggg tgcaggagtt aattcaatcg tttgagaaga atgtgaagcg
      721 ttgttgaaag tgtactcttg taacacaact ttttcgcctc gaatcaattt acataaatcg
      781 ttgtgaaacg ctgagcgatg gatagaagtt ggtaaatcga aatttatctg attgttttca
      841 tccttcaatt gctcttcttt aggaagataa taattgtctt gcgatacaat acaagcagct
      901 ccttcgggca atgcttctcg caagtttttg agaaaggatg tttttcccga agcgcttccg
      961 ccagagattc caactacgta aggtcttctg ctcatcggga cgcgaaatta cattttcttc
     1021 ctcaataaat tcgccctatt aatgcaatat ccaagtaaaa tttacggaat gaattgctta
     1081 cttgtttatc agtcgattaa gcctcaataa ttaattgatt tcgaaatgta tggacaaaaa
     1141 aaatgcagct agaattgaat ctaactgcat ttttaaatgg tttgttatgc ttattggaac
     1201 attgctgctc caagaatctg catattatca actggattca tcaccataac gggtaatttc
     1261 ctttcgttgg caataatatc ttgctcgtga gttccagtaa agtactcggt gaattcacga
     1321 tcttcatcgg tcatgattaa aatcaaatca gcatcttttg cagaagcata aatgataatc
     1381 tctttatgaa atgctgtcga tccgctcgca gtttctactg aataactaca tccgtgttga
     1441 tccaaatact tcttagcaaa cgtgatgttg ttttgcattt tgtgctgaat aaactcatcc
     1501 gtttgatgtt cagctaataa caagaagtga cattggaaca ttttagcaat gtgaatcgcc
     1561 catgccaatt tctgacgact ttcaaccgtg aatgaaaatg gcaaaacaac cgttttcaaa
     1621 tcattgcgtt taggcatatc ctgaacaaca atgaatggaa catccttcgt gtttttaatc
     1681 actttcatgg catgactacc cgtccagcgc tgaagtcctt taataccttt tgttcccatt
     1741 accaccaaag tagcgttgag ttcttcggct aattcaccaa ttccttcgtg aaaatcacca
     1801 cgacgaacta aaacggaaac ttcaattcca ctttcattcg taatatcatc tgcgaaattc
     1861 tgaagtttag acaatgcctc ttcttcttct aatttcaact catgttcctt atcaccccag
     1921 cctttatcga ttaaatgtac tagcgtaatc ggttttttga agatggtcgc atacaattct
     1981 gcatgtgcca atgccggttt agtgatgtca gaaaagtcaa cagggacaag gattgtagcc
     2041 gttaaatcaa gggatttcat tccagaaata gcattattag tttgccgctc gaaattaagg
     2101 tattcagact tactttcgcg tagtcttgaa aaaaattgat ttgcaatgga ggaactgaga
     2161 ggaaacccta aagttattag ggcttggact attttcgact gggctaattc ggtgtatcag
     2221 ctgagtatta cttcggct
//
LOCUS       DAOOQW010000307         2156 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_63137_length_2156_cov_4.630176, whole genome shotgun sequence.
ACCESSION   DAOOQW010000307 DAOOQW010000000
VERSION     DAOOQW010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63137_length_2156_cov_4.630176"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /locus_tag="PK281_11680"
     CDS             complement(<1..627)
                     /locus_tag="PK281_11680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY50048.1"
                     /translation="MKKTSAIEMNFMISQFSKIALFCLLLVMGFHSASARKIYSKAPG
                     GNWTSAGSWTAGIVPAANDTVYIQGGHTISISSNEGSNSTYMFVIVVGTLDMTNNGKL
                     SLSGSSYVIVENSGSILGNGNSDTISIGTGGAEYSGGQGNVTGPSYVGNGHTPASGEG
                     TNGCGCYNSVSSCAITSSAGYTVYVTVTAKSLVKPATCPFGYNYNVTLD"
     gene            683..1459
                     /locus_tag="PK281_11685"
     CDS             683..1459
                     /locus_tag="PK281_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOY50049.1"
                     /translation="MSVIKTHNTSSILIVTISRGEALNALNVDVLDGLHKIIQEAYQN
                     SNVKGIILTGEGEKAFVAGADIKELSSLNKEQAFAMARKGQLLFKQIEDFPKPIIAAV
                     NGFALGGGCELAMACHIRIATENAKFGQPEVNLGIIPGYGGTQRLTQLVGRGKALELI
                     MTADFISAQEAKAIGLVNHVVENRDELMDLATKMMTKILSKSPVAISQVIKSVNAGFN
                     GDEIGYQTEAESFAACAASEDFKEGTAAFVEKRKANFKGV"
     gene            1580..>2156
                     /locus_tag="PK281_11690"
     CDS             1580..>2156
                     /locus_tag="PK281_11690"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOY50050.1"
                     /translation="MGKLKRLAGDTVLYGLGSILPKFLNFLLVLLHTDVFLPAEYGII
                     TNLYAYVTFINVLFTFGMETAYFRFASKPGADEKKIFNLTQTVVVSISLILSLIIIGF
                     AQPIAIQLDIESQSNYVIWIVAILFIDSVVAIPFARLRLQNRPLKFAFGRILNILILV
                     GLNVYFLIYAYDQSIGVAYVFIANLTANVFYL"
BASE COUNT          658 a          418 c          423 g          657 t
ORIGIN      
        1 gtcaagcgta acattatagt tataaccaaa aggacaagtc gctggcttta ctaagctttt
       61 ggcagtaacc gttacgtaaa cagtataacc ggcacttgat gtaatcgcgc aagaacttac
      121 agaattgtaa catccacatc catttgtgcc ctcaccagat gcaggagtat gaccatttcc
      181 aacatagctt ggtcctgtta cgtttccttg acctccagaa tactctgcac cccctgtacc
      241 aattgatatt gtatcactat tgccatttcc taaaatgcta ccactattct caacgatgac
      301 ataagaggaa ccactcaagc ttaacttacc gttattggtc atgtcaagag ttccaacaac
      361 aatcacaaac atataggtag aattgctacc ctcattacta gaaattgaaa tggtatgtcc
      421 tccttgaatg tatacagtat cattagcggc aggtacaatt ccggcagtcc aacttccggc
      481 actagtccaa ttacctccag gggctttaga ataaatcttt cgtgccgaag cagaatgaaa
      541 acccatcacg agaagtaaac aaaagagtgc tattttagag aattgagaaa tcataaaatt
      601 catttcaatc gcagaggtct ttttcataat tattttgagt ccttccaaaa gctggataag
      661 ttgcattact aaatagatcc ccatgtctgt aatcaaaaca cataatacct cctccattct
      721 gatcgtgacc atttcacgag gagaagcatt gaatgcatta aatgttgatg tactggatgg
      781 gctgcacaaa attatacaag aagcttacca aaacagcaat gtgaaaggaa taatcttaac
      841 aggggaggga gaaaaggctt ttgttgcagg agcggatata aaagagcttt catctctcaa
      901 caaagaacaa gcgtttgcaa tggcacgaaa aggccaatta ttgtttaagc agattgaaga
      961 ttttcccaaa ccaattattg cagcagtaaa tggttttgct ttgggaggtg gatgtgagtt
     1021 ggctatggca tgccacattc gtattgcaac agaaaatgcc aaatttggac aaccggaagt
     1081 aaatctggga attataccgg gttatggagg aacacagcga ttaacgcaat tagtaggtag
     1141 aggtaaggct cttgaactga tcatgactgc tgatttcatt tcagcacaag aagcaaaagc
     1201 aattggattg gtgaatcacg tagtagaaaa ccgtgacgaa cttatggatc ttgccacaaa
     1261 gatgatgact aaaattcttt cgaaatcgcc tgttgccatt tcgcaagtca tcaaatcggt
     1321 aaatgctggt tttaatggtg atgagatcgg gtaccaaacc gaagctgaaa gttttgcggc
     1381 ttgtgcagca tctgaagatt ttaaagaagg tacggctgcg tttgtggaga aacgaaaagc
     1441 taattttaaa ggagtgtaat atttaatacg aatagtaatt agtgatgagt gattagcgac
     1501 tgatgatttt tgatttgatt ctttactgaa tgattacgaa ataccaaatc acccttaact
     1561 gataactgat taccgaacaa tgggtaaact caagcggctt gcaggcgata cagtccttta
     1621 tggacttggt tctatcttac ctaagtttct caacttctta ctggttcttt tacacacgga
     1681 tgtttttctt ccggctgagt atggtattat aaccaatctg tatgcctatg ttacctttat
     1741 caatgtgctc tttacgtttg gaatggaaac tgcatatttt cgttttgctt ctaagccagg
     1801 agccgatgag aagaaaattt tcaatcttac ccaaactgtc gtagttagta taagtttaat
     1861 tctctcgtta attattattg gttttgcaca acccatagca attcaactgg atatagaaag
     1921 tcagtcgaat tatgttatct ggattgtcgc tattttattt attgattcag ttgtggctat
     1981 tcccttcgct cgcctccgcc tccaaaaccg tccattgaaa tttgcgttcg gaagaatttt
     2041 aaacattctg attctggttg gccttaatgt ttacttcctg atatatgcct acgatcaatc
     2101 tattggtgta gcgtatgttt ttatcgccaa tctcaccgcc aatgtttttt acctgt
//
LOCUS       DAOOQW010000308         2128 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_64356_length_2128_cov_4.265798, whole genome shotgun sequence.
ACCESSION   DAOOQW010000308 DAOOQW010000000
VERSION     DAOOQW010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64356_length_2128_cov_4.265798"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1223
                     /locus_tag="PK281_11695"
     CDS             <1..1223
                     /locus_tag="PK281_11695"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOY50051.1"
                     /translation="NLKRFLCKQIEMTNKIVRAGIIGSGFAARFHFEALRRVFSVQIE
                     VVGAYSKSPDELVEFTSKRNIKCFESMEELISASDVLHVCTPPSTHEPIAVEVLKLNK
                     HVIIEKPFTGYFGDGTKEFNGDNFDRELGLKKSLESVKRMLDAEQASEGRIMYAENWV
                     YAPAIQKERELIEKTKAQILWIQAQQSHSGSHSSDYGQWRLSGGGSLMGKGCHPLAAS
                     IYLKHVEGRVRNGIPIRPKTVSARTHTITRMSGYKNEGHLRDTYKDIEDYASVHIVFE
                     DGTFADIVASELLQGGVKNYVEVHANNHRTICNIAPNNAMQTYNPREENFNDVYVVEK
                     TGTKQGWSFISPDEAWFNGYQHEMDAFYKSLSFNEPIESGSRLAADVVTTIYSAYVSA
                     DLQGAEVSISIFNR"
     gene            1291..>2128
                     /locus_tag="PK281_11700"
     CDS             1291..>2128
                     /locus_tag="PK281_11700"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOY50052.1"
                     /translation="MVLNKSSDTTPVKKYRWIILSLVFFATTINYLDRQVISLLKDDY
                     LEPLFGWNESDYANIVIAFQITYALGMIGSGFMIDRIGTKLGYALSLTVWSLASIGHA
                     LAKSTFGFMMARGVLGVSEAGNFPAAIKTVAEWFPKKERALAAGIFNSGTNVGAILAP
                     LSVPLIAVTLGWEWAFIITGAIGLIWLIFWWWLYESPQNHKKISKQELDYINSDEPDD
                     TTASDQLAKVKWVTLLGFKQTWAFALLKFFTDPIWWFILFWLPSFLNKQYGMTKMELA
                     FPI"
BASE COUNT          644 a          403 c          490 g          591 t
ORIGIN      
        1 ggaatctgaa aagattcctg tgtaaacaaa tagaaatgac aaataagata gttcgggcag
       61 gaatcattgg ttccgggttt gcagcacgat ttcatttcga ggcgttaaga cgagtcttca
      121 gtgtgcagat tgaagtggta ggtgcctatt caaaatctcc agacgagtta gttgaattta
      181 cttccaagcg aaacatcaaa tgcttcgaga gcatggagga gctcatttca gcatcggatg
      241 ttctgcatgt ttgtacgcca ccttctacgc acgagccaat tgctgttgaa gtattgaagc
      301 tgaataaaca tgtcattatt gaaaaaccat ttaccggata tttcggagac ggaacaaaag
      361 aatttaacgg agacaatttt gatcgtgagc ttggattgaa gaaatcactt gaaagcgtca
      421 agcgaatgct cgatgccgaa caagcaagtg aaggaaggat catgtatgct gaaaactggg
      481 tatatgcacc ggccattcaa aaggaaaggg agctgatcga aaaaacgaaa gcacaaattc
      541 tttggataca ggcgcaacaa tcgcattccg gttcacattc atccgactat ggacaatggc
      601 gtctttccgg tggtggatcg ttaatgggaa agggttgtca cccgttagca gcttctattt
      661 atttaaaaca cgttgaagga cgagttagaa atggaatacc tattcgtccg aaaacggtat
      721 cggcaagaac gcataccatc acaagaatgt cgggatacaa aaatgaagga catcttcgcg
      781 acacgtacaa ggatatagaa gattatgcat ccgtgcatat cgtatttgag gatggaacct
      841 ttgctgatat tgttgcaagc gaattattgc agggcggtgt aaaaaattat gtcgaagtcc
      901 acgccaataa tcatcgcacc atttgcaaca tcgcaccgaa taacgccatg cagacgtaca
      961 atccgaggga agaaaatttt aatgacgtat acgtagtaga aaaaaccgga accaagcaag
     1021 gatggtcatt tatatctcct gacgaagcat ggttcaatgg ctatcaacac gagatggacg
     1081 ctttttataa atcattatca tttaatgaac caattgaaag cggcagtcgg ttggcagcgg
     1141 acgtagtgac cacgatctat tctgcctatg tatcagcgga tctccagggt gctgaagtgt
     1201 ctatttcaat ttttaacaga taagatttta gtacagatga tactcaactc attaatcgtg
     1261 gcaacatctt aaactagtga aatctaaaaa atggtgttaa ataaatcctc cgatacaact
     1321 cccgtaaaaa aatacagatg gattatactt tcattggtat tttttgcgac caccattaat
     1381 tacctcgacc gccaggtaat cagtctccta aaagatgatt acctcgagcc actgtttggc
     1441 tggaatgaat ccgactacgc caacattgtt atagcattcc agatcaccta tgcattgggt
     1501 atgataggtt ctggttttat gatcgatcgt attggaacca aactgggtta cgcactttca
     1561 ttgacggtgt ggagtttggc ttcaattggt catgcgttgg ctaaatcaac ttttgggttt
     1621 atgatggcga ggggtgtgtt aggagtgagt gaggctggaa attttccagc cgccatcaaa
     1681 accgttgctg aatggtttcc aaaaaaagaa cgggcactgg cagccggtat ttttaattca
     1741 ggcacaaatg taggcgccat tttagcaccg ctctctgttc cactaattgc ggttacgtta
     1801 gggtgggagt gggcgtttat tattaccggt gccattggac ttatctggtt gatcttttgg
     1861 tggtggttgt acgagagtcc acaaaatcat aaaaagattt caaaacaaga attggattac
     1921 atcaacagtg acgaacctga tgatacaaca gcgtctgatc agcttgcaaa agttaagtgg
     1981 gttaccctgt tgggtttcaa acagacgtgg gcgtttgcat tgttaaagtt ttttactgac
     2041 cccatctggt ggtttatttt attctggctc ccttcattcc tgaataagca atatggcatg
     2101 accaaaatgg aattagcttt tccaatag
//
LOCUS       DAOOQW010000309         2119 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_64752_length_2119_cov_5.094961, whole genome shotgun sequence.
ACCESSION   DAOOQW010000309 DAOOQW010000000
VERSION     DAOOQW010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64752_length_2119_cov_5.094961"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..969)
                     /locus_tag="PK281_11705"
     CDS             complement(<1..969)
                     /locus_tag="PK281_11705"
                     /inference="COORDINATES: protein motif:HMM:NF040245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HOY50053.1"
                     /translation="MADTLEGQSLKEKLLAGFHIRKAALLPDNTILNGFRDKAAESFS
                     RLGFPGKKHEEYRYLQVEKLFTSELNQTLSPEEIDLPNESDVLNHPLLVEDAYRLVML
                     NGIFSKKLSRIEGLPTGVVMGSLAEALRNNDAHALTNLGTLAKADSDSFLALNSALFQ
                     DGIYCYIPSNCKLPKPVQLIELVSSPEALLIQPRVLFVSDESSEAEVIQTSISTKGNT
                     SIASNSVTEILVEKNAQVNWITLQSEEHTAGHFHTTEAHVKQHGKFETVTISLGSGML
                     RNNLNIVLDAPECEAHLYGYYHPVNTQIVDNHTLVDHRYPRCESNEL"
     gene            complement(981..1736)
                     /gene="sufC"
                     /locus_tag="PK281_11710"
     CDS             complement(981..1736)
                     /gene="sufC"
                     /locus_tag="PK281_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019385971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HOY50054.1"
                     /translation="MLSIRNLHARVEEKEILKGFSLDVKPGEVHAIMGPNGSGKSTLA
                     SVLAGREDYQVTGGEVIFEGKDLLALAPEDRAREGIFLAFQYPVEIPGVSITNFLKTA
                     IAEQREYRGLPPMEPKEFLQLMKEKKAYVEIESNLTNRSVNEGFSGGEKKRNEIFQMA
                     MLNPKLAILDETDSGLDIDALRVVANGVNRLKSPENSAIVITHYQRLLDYIVPDVVHV
                     MYDGRIVKTGGKELALELEEKGYDWIKKELAAL"
     gene            complement(1926..>2119)
                     /locus_tag="PK281_11715"
     CDS             complement(1926..>2119)
                     /locus_tag="PK281_11715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY50055.1"
                     /translation="NHSRIPYLTPDGQNKTLEGDLSMTLFTNNPFSVYDTIKYEVTVV
                     DRALHRSNQIKTADIILSK"
BASE COUNT          607 a          463 c          393 g          656 t
ORIGIN      
        1 caattcgttg ctttcgcagc gcggatagcg atgatcaacc aaggtatgat tatcaacaat
       61 ttgcgtattc accggatgat aataaccata caaatgcgct tcacattcgg gagcatcgag
      121 cacgatattt aagttattac gcaacatgcc cgaacctaaa ctaatcgtta cagtttcgaa
      181 tttgccatgt tgcttcacat gagcttcggt ggtatgaaaa tgtcctgcag tatgttcttc
      241 cgattgaaga gtaatccagt ttacctgagc atttttctcc accaaaatct ccgtcacact
      301 gttactagca attgacgtat ttcctttggt actgatggat gtttgaatga cttctgcttc
      361 tgaagattca tccgaaacaa ataacacgcg aggttgaatc aataaagctt ctggactact
      421 taccaattca attagttgaa ccggtttagg tagtttacaa tttgatggga tgtaacaata
      481 aattccatct tgaaacaacg cagagtttaa tgctagaaac gaatcagaat cagcttttgc
      541 aagtgttccc agattagtta atgcgtgggc atcgttgttt cgtaatgctt ctgctaaact
      601 tcccatcact acacctgttg gcaaaccttc gattcgggat agtttttttg aaaatatccc
      661 atttaacatc acaagtcgat atgcatcttc aaccaataaa ggatgattga gtacatcaga
      721 ttcgttaggt aaatcgattt cctctggact gagtgtttga ttcagttcag atgtaaatag
      781 tttttcaact tgcagatagc ggtattcctc gtgttttttc cctgggaaac ctaaacggga
      841 aaacgattct gctgccttat cccgaaagcc attaagaata gtgttatcgg gcaataaggc
      901 tgctttccgg atatgaaatc cagctagcag cttttctttt aacgattgtc cttcgagtgt
      961 atcggccatg ttgcggacaa ttataaagca gctaattctt tcttaatcca gtcgtaacct
     1021 ttttcttcca gctcgagtgc taattctttt ccgccagttt taacaatgcg accatcatac
     1081 atcacatgca ccacgtcagg aacgatataa tcgagcaaac gttggtagtg agtaatgaca
     1141 attgcagaat tttcgggcga tttcaagcgg tttacaccat tggcaaccac acgtaaagcg
     1201 tcaatatcga gtcctgaatc ggtttcatct aaaatggcca atttcggatt gagcattgcc
     1261 atttgaaaaa tctcatttcg tttcttttca ccacccgaga aaccttcgtt aacagagcga
     1321 ttggtcaggt tggattcaat ttctacataa gcctttttct ccttcatcaa ctgaagaaat
     1381 tcttttggct ccatgggagg aagtccacgg tattcgcgtt gttcagcgat agcagtcttc
     1441 aaaaagtttg taatactaac gcccggaatc tccaccggat actgaaaagc aagaaaaatt
     1501 ccttcgcggg cgcgatcttc aggcgctaaa gcaagtaagt cttttccttc gaaaataact
     1561 tctcctcccg ttacttgata atcttctctc ccagctagaa ccgaagccaa tgtggatttc
     1621 ccagaaccat tcggtcccat aatcgcatga acttctcccg gttttacatc taatgaaaaa
     1681 cccttcagaa tctctttttc ttctacacgt gcgtgcaggt tacgaatact cagcatttag
     1741 catgtttttg gaacagcctt gtttaaaacc gttctaaata aggccgcaaa ggtaacacaa
     1801 tcgcaacgat taggttcatt tcaattttga aaaaagcaag gttttcattt caaaatgatc
     1861 ctgaacttcg gcctgtttcc ctttcagaat gagcgattcc tatgataaat tctcctaagc
     1921 agcaattatt tgctaagaat aatatcggcc gtttttatct gattacttct atgcaaagcc
     1981 cgatcaacaa ccgttacttc atacttaata gtatcgtaaa ccgaaaaagg attattggta
     2041 aataatgtca tgctcaaatc accctcgagc gtcttgtttt gtccatcagg agtgagatac
     2101 ggaatgcgac tatgattac
//
LOCUS       DAOOQW010000310         2088 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_66274_length_2088_cov_2.434825, whole genome shotgun sequence.
ACCESSION   DAOOQW010000310 DAOOQW010000000
VERSION     DAOOQW010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66274_length_2088_cov_2.434825"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2088)
                     /gene="rpoC"
                     /locus_tag="PK281_11720"
     CDS             complement(<1..>2088)
                     /gene="rpoC"
                     /locus_tag="PK281_11720"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002668742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOY50056.1"
                     /translation="EVTEKKVRRERMGHIQLVVPVAHIWYFRSLPNKIGYLLGMPTKK
                     LDSIIYYEKYVVIQAGIADNTATAATGELLIAQGELISEEEYLDLLEKLPRENQLLED
                     SDPNKFIAKMGAEAIYDMLTRLDLDALSYDLRHKANTDTSQQRKNEALKRLQIVESFR
                     ASKMRNKPEWMIVKIVPVIPPELRPLVPLDGGRFATSDLNDLYRRVIIRNNRLKRLIE
                     IKAPEVILRNEKRMLQESVDSLFDNSRKSSAVKTEANRPLKSLSDSLKGKQGRFRQNL
                     LGKRVDYSARSVIVVGPELKMHECGLPKDMAAELFKPFVIRKLIERGIVKTVKSAKKI
                     VDRKDPVIWDILEYVMKGHPVLLNRAPTLHRLGIQAFQPKMIEGKAIQLHPLSCTAFN
                     ADFDGDQMAVHLPLGNEAVLEAQMLMLASHNILNPANGAPITVPSQDMVLGLYYITKP
                     RTGALGEGLIFYSPEEAEIAYNEKKVTLHAWIKVRTKDLNENGEIVTKLIETTVGRIL
                     FNKCVPIEVGYINELLSKKSLRDIIGNVIDVCGVARTAQFLDDIKDLGYYMAFKGGLS
                     FNLGDVIIPPEKEVLVKEGNAEVEEIMNNYNMGFITNNERYNQIIDTWTHVNSKLSNI
                     LMKQLSSDNQGFNSVYMMLDSGARGSKEQIRQLSGMRGLMAKPQKSGATGGEIIENPI
                     LSNFKEGLSVLEYF"
BASE COUNT          581 a          447 c          408 g          652 t
ORIGIN      
        1 aaaatactca agtaccgaaa gaccttcttt aaagtttgat aaaatcgggt tttcgataat
       61 ttcacctccg gttgcacctg atttttgagg ttttgccatc aaaccacgca tacctgaaag
      121 ctgacgaatt tgttccttag aaccacgcgc tccagaatcc aacatcatgt acacagagtt
      181 gaatccctga ttgtcgcttg ataattgttt catcaaaata ttcgacaatt tcgagtttac
      241 gtgtgtccat gtatcgatga tctgattgta acgttcgttg ttggttatga atcccatgtt
      301 gtagttattc ataatttctt caacttcggc attaccttct tttaccaaaa cttctttttc
      361 gggtgggata ataacgtcac ctaagttaaa cgacaatcca cctttgaaag ccatgtagta
      421 acccaaatct ttgatatcat cgaggaattg agctgtacgg gcaactccgc atacatcgat
      481 aacattaccg ataatatcac gaagcgattt tttcgaaagt aattcgttga tgtaacctac
      541 ttcaatcgga acacatttgt tgaataaaat tcgtccaaca gtagtttcga ttaattttgt
      601 tactatttcg ccattttcat ttaaatcttt ggttcttacc ttgatccatg catgaagcgt
      661 aactttcttc tcattataag ctatttcagc ttcttcagga gaatagaaaa ttaagccttc
      721 gcctaaagcc cctgtgcgtg gtttcgtaat ataatacaat ccaagcacca tgtcctgaga
      781 aggtaccgta ataggagctc cgttggctgg gttcaaaata ttatgtgatg caagcataag
      841 catttgagct tcgagcactg cttcgtttcc aagaggcaag tgaacggcca tctggtcacc
      901 atcaaagtcg gcgttgaaag ccgtacacga aagtgggtga agctgaatag cttttccttc
      961 aatcattttg ggctggaaag cctgaatacc caaacggtgc aaagtcggag cacggttgag
     1021 cagtacagga tgtcctttca tcacgtactc cagaatatcc cagataacag gatccttgcg
     1081 atcaacgatt ttcttcgctg atttcacagt ttttacaatg ccgcgttcaa tcagtttgcg
     1141 aataacaaac ggtttgaaaa gttcggctgc catatcttta ggtaagccgc actcgtgcat
     1201 cttcaattcc ggtccaacga cgattaccga acgtgcagaa taatcaacac gtttacctaa
     1261 taagttctga cggaaacgtc cttgtttccc tttcaacgaa tctgaaagag atttcaacgg
     1321 gcgattggct tctgttttaa cagcgctcga tttacgtgag ttatcgaaca atgagtcaac
     1381 agattcctga agcatacgtt tttcattacg taaaattact tctggcgctt taatttcaat
     1441 cagtcgtttc agacggttgt tacggataat tacgcggcga tacaggtcgt tcaaatcgga
     1501 ggtggcaaaa cgaccaccat ccaatggcac caatggacgt aattcgggcg gaataaccgg
     1561 aacaattttg acaatcatcc attctggttt attgcgcatt ttcgaagcgc ggaacgattc
     1621 tacaatttgc agacgtttaa gtgcttcgtt tttacgttgc tgagaagtat cggtatttgc
     1681 tttgtgacgt aaatcgtatg ataaagcatc taaatcgaga cgcgttagca tatcgtagat
     1741 agcttctgca cccattttag caatgaattt atttggatcg ctgtcttcaa gtaattgatt
     1801 ttcgcgtggc aatttttcga gtaaatcgag gtattcttct tccgaaatta actcgccctg
     1861 agcaatcagg agttcacctg ttgcagcagt agcagtattg tcggcaatac ctgcctgaat
     1921 aaccacatat ttttcgtaat atatgattga atctaatttt tttgttggca ttcccagcaa
     1981 ataaccgatt ttattcggca atgaacggaa gtaccaaatg tgagctacag gcactaccaa
     2041 ctggatgtgt cccattcgct cgcgacgaac ttttttctct gttacttc
//
LOCUS       DAOOQW010000311         2043 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_68440_length_2043_cov_4.917002, whole genome shotgun sequence.
ACCESSION   DAOOQW010000311 DAOOQW010000000
VERSION     DAOOQW010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68440_length_2043_cov_4.917002"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="PK281_11725"
     CDS             <1..464
                     /locus_tag="PK281_11725"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HOY50057.1"
                     /translation="NLGISKNTVDRYLDLLQKVFVIQKLQGFSRNLRKEVTRHARYYF
                     TDNGIRNALINNFNALPLRNDVGELWENYLMMERLKFQSYTNLHSLNYFWRTYQQQEI
                     DWVEDRNGKLYAYECKWKDSGKVKIPSAWKDAYPESEFSVISPSNYLNWIQ"
     gene            461..820
                     /locus_tag="PK281_11730"
     CDS             461..820
                     /locus_tag="PK281_11730"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HOY50058.1"
                     /translation="MKTRLLAFRFAIQGIVHGLKTEAHLRFHVLAAILVIGFGCWFSI
                     SPIEWCLVTIAMGIVISAELINAALERLADRITKEEDPLIGQAKDLGAGAVLIVSIMA
                     VCIAFGVFGPYIYRLLS"
     gene            893..1624
                     /locus_tag="PK281_11735"
     CDS             893..1624
                     /locus_tag="PK281_11735"
                     /inference="COORDINATES: protein motif:HMM:NF018766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HOY50059.1"
                     /translation="MTPYFVRRLHVYFKSHNRAGVAISFSASIILSFVLLAARIWRTG
                     ELTYLFLVWNLFLAVIPLGLSTLLTVYRLRHKSHLISYSLTITWLLFLPNAFYIITDL
                     FHLYPRPGVPQRFDLVLILSFAWNGIMLGCASIFDMHALIKERFTWLTGWLFAGTTLF
                     LSSFGIYLGRFDRWNSWDLLHHPIRLFSDVLHYISTPSDYPSVWGVTLCYTLLMWVLY
                     ATVYQLTRVQSGRYRAEMVADKTLR"
     gene            complement(1684..>2043)
                     /locus_tag="PK281_11740"
     CDS             complement(1684..>2043)
                     /locus_tag="PK281_11740"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY50060.1"
                     /translation="IPGETSALFTPEPVNASYFVQTTAPNGCSAISEPFHYNVDGFEQ
                     INSTSLNFYPNPANELIFIQNSGISTSFQLCDITGRILKTSSITTNSIISVSLGDLTP
                     GIYWLKTPSNTQRLVIF"
BASE COUNT          533 a          368 c          481 g          661 t
ORIGIN      
        1 ggaatttggg aataagtaaa aacacggttg atcgttacct cgatttgcta cagaaagtgt
       61 tcgtcattca aaagcttcaa ggatttagca ggaatttgcg gaaagaagtc acccgacatg
      121 cacgttatta ctttacagat aacggaattc gaaatgcgct gataaataat ttcaatgcgc
      181 tacccttgcg aaatgacgta ggtgaacttt gggaaaatta cctgatgatg gaacggttga
      241 agtttcagtc atacaccaat ttacattcgt tgaattattt ctggcgcacc tatcagcaac
      301 aggaaattga ttgggtggaa gatcgcaatg gaaagctgta tgcgtatgaa tgcaaatgga
      361 aagattcagg taaagtgaaa attccttcgg cttggaaaga tgcctatcca gaatctgagt
      421 ttagcgttat ttctcctagc aattacctca actggataca atgaaaacca ggcttttagc
      481 atttcgattt gccattcaag gtattgtgca tggcttgaaa acagaagcac atctgcgttt
      541 tcacgtactg gcagcaattc ttgttattgg ttttggttgc tggttttcta tttctcccat
      601 cgaatggtgc ttggtaacaa ttgctatggg catagtgatt tcggctgagt tgattaatgc
      661 tgcattggag cgattagccg accgaattac gaaagaagaa gatcctttga ttggtcaagc
      721 caaagattta ggagcaggtg cagttctaat cgtgtcgatt atggctgtat gcattgcctt
      781 tggggtgttt ggtccttata tctatcgtct gttgagctga agaatcgtta aaaaatcata
      841 atgttggcac tttagcagga cggtaaacgt taaaggagct cgcaattgat ttatgactcc
      901 ttatttcgtc cgtcgtttac acgtttattt caaatcacat aaccgagctg gcgtagctat
      961 tagcttttca gcttcgatta ttttaagctt tgttttgctg gcagctcgca tttggagaac
     1021 gggcgaactc acctatcttt ttctagtgtg gaacctgttt ttggcagtca ttccattggg
     1081 tttatctact ttgttaacgg tgtatcgttt gcgccataaa agccatttga tttcgtatag
     1141 tttgacgatt acttggttgc tctttttacc gaatgccttt tacatcatta ccgatttatt
     1201 ccacctgtat cctcgaccgg gcgtgccgca gaggttcgat ttagtcttga ttttatcgtt
     1261 tgcatggaat ggaattatgc tgggttgcgc ttctatattc gacatgcatg ccttgattaa
     1321 ggaacgattt acgtggttga caggttggtt gtttgcaggc acaacgctgt tcttgagcag
     1381 ctttggaatt tacctcggtc ggtttgatcg ctggaattct tgggatttgt tgcatcatcc
     1441 gatacggtta tttagtgatg tgttgcatta tatcagcaca ccttcggatt acccaagtgt
     1501 ttggggcgtt actttatgtt atacgttgtt gatgtgggtg ttgtatgcaa cagtttatca
     1561 attaacgcgt gtgcaaagtg ggcgctatcg tgccgaaatg gtagctgata aaacacttcg
     1621 ttagcaatac cttggcttac aaaacgacag aatggtttag taatggaagc ccccgatcac
     1681 ctatcaaaaa atcactaaac gttgtgtgtt gcttggggtt ttaagccagt aaattccagg
     1741 tgttaagtca cctaaactca cgctaatgat gctattagta gtgatactgc tagtttttaa
     1801 gatgcgaccg gtgatatcgc aaagctgaaa cgaagttgaa attccagagt tttggatgaa
     1861 aatcaattcg ttagcaggat tgggatagaa attcaggctg gtcgaattga tttgttcgaa
     1921 accatcaacg ttataatgga acggttccga aatagctgaa catccattgg gcgctgtggt
     1981 ttgaacaaag tagctggcat tcaccggttc gggtgtgaat agcgctgatg tttcacccgg
     2041 aat
//
LOCUS       DAOOQW010000312         2032 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_68996_length_2032_cov_4.268589, whole genome shotgun sequence.
ACCESSION   DAOOQW010000312 DAOOQW010000000
VERSION     DAOOQW010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68996_length_2032_cov_4.268589"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..646)
                     /locus_tag="PK281_11745"
     CDS             complement(<1..646)
                     /locus_tag="PK281_11745"
                     /inference="COORDINATES: protein motif:HMM:NF013214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HOY50061.1"
                     /translation="MYQDPIDQLTLSQSSDTAQAAFMTKVYGWMTIALVITALAAMYT
                     AMTPALIEFIFSSKLVFYGLIIGELLLVGSLVMAVQKMSPMVASLVFFGYAALNGLTL
                     ASIFFLFTAGSIALTFFITAGTFAVMSIYGYTTKADLTRIGNLALMALVGVIIASIAN
                     YFLQNETLYWIISYLGVAIFVGLVAYDTQKIKNISATMDIQSSEGKRASVIGALT"
     gene            complement(765..>2032)
                     /locus_tag="PK281_11750"
     CDS             complement(765..>2032)
                     /locus_tag="PK281_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018943577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HOY50062.1"
                     /translation="LAERMRPSTLEGYVGQTHLLGEGSVLAKLISQKLPLPSLLLWGP
                     PGVGKTTLARLLAEASEAPFFTLSAIASGVKEVREIIDMAARQGGLLRKPVLFIDEIH
                     RFSKSQQDSLLGAVERGIVTLIGATTENPSFEVIPALLSRCQVYVLQSLGKEELLGML
                     HRSMREDRFLKFREIQLVETDALMRYSGGDARKLLNLFELVVNTHTGDDPVQITNQSV
                     TAVLQENQARYDKSGEQHYDIVSAFIKSMRGSDPNAAVYYLARMIEGGEDPKFIARRM
                     LILSSEDIGNANPTALIMANNCFQAVNVIGYPECSLILSQTAIYLACSAKSNASYKAI
                     GAARKLVEETGDLPVPLHLRNAPTKLMKELGYGDNYQYAHQFENNFIDLEFLPEQIKG
                     NVLFDPGANARENEMREHLRRLWKEKYGY"
BASE COUNT          612 a          426 c          396 g          598 t
ORIGIN      
        1 aagttagcgc accaattaca gaagcacgtt ttccttcgct cgactgaata tccattgtag
       61 cactgatatt tttgattttt tgcgtatcgt atgctaccaa tccaacgaag attgcaacac
      121 ctaaatagct gataatccaa taaagtgttt cattttgaag gaagtagtta gcaatagatg
      181 caatgataac acctactaaa gccatcaatg ctaaatttcc gattcgagtt aaatcagctt
      241 tggtagtgta tccataaatg ctcatcaccg caaatgttcc tgctgtaatg aaaaacgtta
      301 aggcgataga acctgcagta aacaagaaaa atatagatgc gagtgtaagt ccgtttaatg
      361 ctgcatatcc gaagaaaact aaagaagcaa ccatcggcga cattttctgt acagccatta
      421 caagtgaacc aaccagtaga agttctccaa ttattaatcc gtaaaatacc aatttactgc
      481 tgaagataaa ttcaatcaaa gcgggtgtca tggctgtgta catagccgct aatgcagtaa
      541 taactaaggc gatagtcatc cagccatata ctttagtcat aaacgctgcc tgagcagtat
      601 cgctgctttg gctgagtgtt aactgatcaa ttggatcctg atacatagtg ttttgttgtt
      661 tgggttaaag gtatatagaa gcgagtaatc tgaaaaacaa ttgtcgatta atgcaagaat
      721 tagaatttac tcgtttttcg atttatgaag taggattgta aatcttagta tccatacttc
      781 tctttccaaa gccttctgag gtgttctctc atttcatttt cacgcgcatt ggcacccgga
      841 tcgaaaagta cattcccctt gatttgttca ggtaggaact ctaaatcgat aaaattgttt
      901 tcgaactgat gtgcatattg gtaattgtcg ccatatccca gctctttcat gagtttggtt
      961 ggcgcatttc tgaggtgaag tggaaccggt aaatcgcctg tttcttccac taatttccga
     1021 gcggctccaa tggctttata ggatgcatta ctctttgcag agcaggcaag ataaatggca
     1081 gtttgactta gaattagcga gcattcagga taaccaatca cattcactgc ttgaaaacag
     1141 ttatttgcca taattaaagc tgtaggattc gcattaccaa tgtcttctga tgatagaatc
     1201 aacatacgtc gtgcaatgaa tttaggatct tctccacctt caatcatacg cgctaaataa
     1261 tacaccgctg cattgggatc gcttccccgc atacttttga taaaagctga aacaatgtcg
     1321 taatgctgtt ctcccgattt atcgtaacga gcttgattct cttgtaagac agccgttaca
     1381 ctttgattgg taatttgaac gggatcgtca cctgtatgtg tattgacaac taattcgaat
     1441 aaattcaaca gtttgcgagc atcaccaccc gaatagcgca tcaaggcatc ggtttctacc
     1501 aattgaattt ctcggaattt caagaaacga tcttctctca tcgaacgatg caacattccc
     1561 aacaattctt cttttccaag cgattgaagc acataaacct gacaacgcga caaaagtgct
     1621 ggaatcactt caaacgaagg gttttctgta gtggcgccaa tcaaggttac aattcctcgt
     1681 tcaacggcac ccaataacga atcttgttgc gatttactaa agcgatgaat ctcatcgata
     1741 aataaaactg gcttgcgtaa caaaccacct tgtcgggcgg ccatgtcgat aatctcgcga
     1801 acttctttaa cgccagaagc aattgcacta agagtgaaaa aaggtgcttc gcttgcttct
     1861 gctaaaagtc gcgcaagagt agttttaccc acacctggag gtccccagag taggagagaa
     1921 ggcaatggta gtttctgact tatgagtttt gctagaaccg aaccttcacc taataagtga
     1981 gtttgtccaa cgtatccttc taaggtcgaa ggacgcattc tttctgcaag tg
//
LOCUS       DAOOQW010000313         2028 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_69212_length_2028_cov_4.213887, whole genome shotgun sequence.
ACCESSION   DAOOQW010000313 DAOOQW010000000
VERSION     DAOOQW010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69212_length_2028_cov_4.213887"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            234..1217
                     /locus_tag="PK281_11755"
     CDS             234..1217
                     /locus_tag="PK281_11755"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006257250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HOY50063.1"
                     /translation="MRTIQFREALREALSEEMRRDERVFLMGEEVAEYDGAYKVSQGM
                     LAEFGPKRVIDTPIAELGFAGIGVGAAMNGLRPVIEFMTFNFSLVAIDQVINSAAKMM
                     SMSGGQFTVPIVFRGPTASAGMLSSQHSQAFDNWYANTPGLKVIVPSNPADAKGLLKS
                     AIRDNDPVIFMESEQMYGDKGEVPEGEYLIEIGKADIKRAGNDVTIVSFGKIMKACYL
                     AADELEKEGVSCEIIDLRTIRPIDYDTVIESVKKTNRLVIVEEAWPLGSIATEVTFKV
                     QKDAFDYLDAPILRVTSADVPLPYAPTLIDAALPNPSKIIAAVKQVMYTAK"
     gene            1343..>2028
                     /locus_tag="PK281_11760"
     CDS             1343..>2028
                     /locus_tag="PK281_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HOY50064.1"
                     /translation="MENFIYIIPALGVIGLIWMAIQSAWVSKQDAGDAKMQELAGYIA
                     DGAMAFLKAEWKVLSFFVVFVAILLAYSGTVHEVNGKEIHSSWIISIAFVIGAVFSAT
                     AGYIGMKVATKANVRTTQAARTSLKQALKVSFTGGTVMGLGVAGLAVLGLGGLFIVFL
                     KMFHVVSVDSNEMRTAIEVLTGFSLGAESIALFARVGGGIYTKAADVGADLVGKVEAG
                     IPEDDVRNPAT"
BASE COUNT          579 a          361 c          445 g          643 t
ORIGIN      
        1 agaaaaggtt gaattgttag gtgcaattta aaagttatgc cttttacttt gacatttatc
       61 tacttatgta ttgaaaaaaa acttccaaat aagcttgcga attggcatat cgataatttt
      121 taacacttga aaggtaaaag tcgttttata tcatcaaacc actaaaaaaa attgggtgtt
      181 tacttgtctg aggtatttct ttcttagatt tgccaccgtt tgaaaccaaa actatgagaa
      241 caatacaatt ccgggaagct ttacgtgaag cacttagcga agaaatgcgt cgtgatgaac
      301 gtgtcttttt aatgggtgaa gaagtagccg aatacgatgg tgcatacaaa gttagtcaag
      361 gtatgcttgc cgaattcggt cccaaacgcg taatcgatac tcctatcgcg gaattaggtt
      421 ttgcaggaat tggtgtagga gcagctatga atggcttgcg tcccgttatc gaattcatga
      481 cgtttaactt ctcacttgtt gctatcgacc aagtgattaa ctcggctgct aaaatgatgt
      541 cgatgtcggg cggacaattt actgttccaa tcgtatttcg cggtccaact gcttctgctg
      601 gaatgttaag ttctcaacat agtcaggctt ttgataattg gtatgctaat actccaggat
      661 tgaaagttat tgttccctcg aatccggctg atgcgaaagg cttacttaaa agtgctattc
      721 gcgataacga tccagttatt ttcatggaaa gtgagcagat gtatggcgat aaaggtgaag
      781 ttcctgaagg tgagtacctg attgaaattg gaaaagccga catcaaacgt gctggtaatg
      841 atgtaacaat agtttccttt ggtaaaatca tgaaagcctg ttatctagct gctgatgaac
      901 tagaaaaaga aggtgtttct tgtgagatta tcgacttacg caccattcgt ccaatcgatt
      961 atgataccgt tattgaatcg gttaaaaaaa ccaaccgttt ggttattgta gaagaagctt
     1021 ggccacttgg tagtattgca actgaagtaa cgtttaaagt tcaaaaagat gcattcgatt
     1081 acctcgatgc tccaattctt cgtgtgacat ctgcggatgt tcctctgcct tatgctccaa
     1141 cacttataga cgcagctttg ccgaatccaa gcaaaattat tgctgctgtg aagcaagtaa
     1201 tgtatactgc taaataagta gaatacattc attatcaacg cgtaacaagg atgattgagc
     1261 ggaacgtttt tccactcaaa tattcttgta tttttgcgct cccaatttct attgttaaga
     1321 aacgttaaat ctcaaatctc atatggagaa cttcatttac atcattcctg ctctaggcgt
     1381 gattggctta atctggatgg caattcagtc tgcttgggtt agtaagcaag atgctggcga
     1441 tgccaaaatg caagaattag ctggctacat tgccgacggt gccatggctt tcctgaaagc
     1501 tgaatggaaa gtactaagct ttttcgtcgt ttttgttgct atccttttag catattcagg
     1561 aaccgttcat gaagttaatg gtaaagagat tcactctagc tggatcattt caattgcctt
     1621 tgtcattgga gctgtatttt cagcaacagc tggatatatc ggaatgaaag tagctaccaa
     1681 agctaacgtt cgtactacac aagctgctcg tactagctta aaacaagctt tgaaagtttc
     1741 ctttactggt ggaactgtta tgggcttagg tgttgctggt ttagctgttc taggtttagg
     1801 tggattgttc atcgtattcc ttaaaatgtt ccatgttgtt agtgtcgaca gcaatgaaat
     1861 gagaactgct atcgaagtat tgactggttt ctcattagga gctgaatcaa tcgcattatt
     1921 tgctcgtgtt ggtggtggta tctatactaa agctgccgat gtgggagctg acttagtagg
     1981 taaggtagaa gctggaattc cagaagatga tgtgcgtaac cctgcaac
//
LOCUS       DAOOQW010000314         2006 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_70372_length_2006_cov_3.602255, whole genome shotgun sequence.
ACCESSION   DAOOQW010000314 DAOOQW010000000
VERSION     DAOOQW010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70372_length_2006_cov_3.602255"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="PK281_11765"
     CDS             <1..285
                     /locus_tag="PK281_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese ABC transporter ATP-binding protein"
                     /protein_id="HOY50065.1"
                     /translation="PFVGVDAATEQSIMDMLRRMKAAGKTIVVVHHDLHTAPEYFDWL
                     VLLNASLIASGPLAEVFTDELLKVTYSGKLTVLSQVGDQLRERNFPVRES"
     gene            282..1580
                     /locus_tag="PK281_11770"
     CDS             282..1580
                     /locus_tag="PK281_11770"
                     /inference="COORDINATES: protein motif:HMM:NF013143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HOY50066.1"
                     /translation="MNSQQLIDFLLLREPNVLLVVAGSLILCGMAAAVGCFTFLRKRA
                     LIGDAISHSVLPGVCLAFILTGEKSPLYFLIGAILSGMLSLVIMEGLSRSRMVRTDTS
                     IALMLSVFFGTGIVLLTSIQHGGNAAQAGLDKFLFGKAASITQEDIWLFSISAASILG
                     ILILFFRGFTAISFDEEYAVSLGFPVKWLRFCLSFITVWAVAVGIQAVGVVLMSALLI
                     TPALAARMWTHSLRIMIALALVFGMISGYIGAFVSYTAPAMPTGPWIVVIATLLAGIS
                     MLFAPQRGLIAQWIERSKNRNKTLEENILKLFYQIGEKAGEFRKPVLEEDLINYRAFS
                     NQQLRKGLKLLLSKKLLTMNLHAWQLTEQGVLEAQRIVRLHRLWELYLQQYLNLDPDH
                     VHDDAEAMEHVITPEIEAQLDELLGYPVIDPHNTLIPPRV"
     gene            1577..>2006
                     /locus_tag="PK281_11775"
     CDS             1577..>2006
                     /locus_tag="PK281_11775"
                     /inference="COORDINATES: protein motif:HMM:NF013143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HOY50067.1"
                     /translation="MTLNDFWIVLTASLISLCCVIPGTFLVLRRMAMFGDAISHAVLP
                     GLVIAYMISGSRASMYMLPGAAASGLLVTFLIEWVNKRMRIQSDAAIGLIYTFLFAIG
                     VILISAYTAQTDLDQDCVLYGEIAYVPFDLLISDAGNSLGP"
BASE COUNT          538 a          358 c          457 g          653 t
ORIGIN      
        1 ccatttgttg gagttgatgc cgcaaccgaa caatcgatca tggatatgct tcgtagaatg
       61 aaagcagcag gtaaaaccat cgttgtcgtt catcatgatc ttcatactgc acctgaatat
      121 ttcgattggt tggttttatt aaatgcttca cttatcgcta gtggcccatt agccgaagta
      181 tttacagacg aattattgaa agtaacctat agcggaaaac taacagtatt atcgcaagta
      241 ggtgatcaat tacgcgaacg aaactttccg gtacgcgaat catgaacagt cagcaactca
      301 ttgactttct tctattgaga gaacccaatg tactactcgt agtagcgggt tcactcattt
      361 tatgtggaat ggctgctgca gtgggttgtt ttaccttttt acgcaaacgc gctttaattg
      421 gagatgccat ttctcacagc gttttaccgg gtgtttgttt agcattcata cttactggag
      481 agaaaagccc gctgtatttt ttgattggtg ctatacttag tgggatgctc agtttggtca
      541 ttatggaagg attaagcagg agtcgtatgg ttagaaccga cacttccata gcacttatgt
      601 tatcggtgtt tttcgggaca ggtattgttt tgctcacatc cattcaacat ggaggtaatg
      661 cagcacaggc cggattagat aaattcttat ttgggaaagc tgcatctatt acacaagaag
      721 atatttggtt gttttctatt tcagcagcat ccattttagg aattcttatt ctcttttttc
      781 gaggatttac agccatttca tttgatgaag aatatgctgt atcgcttggt tttcctgtta
      841 agtggctgcg attttgtctt tcgtttataa ctgtttgggc agtcgctgtt ggaatacagg
      901 cagttggtgt tgtgttaatg tcggcattat taattacacc cgctttagca gctcggatgt
      961 ggactcattc acttcgaatt atgatagcat tagccttagt attcgggatg atttcgggat
     1021 atatcggggc ttttgtttcc tatactgcac ctgcaatgcc gacaggacct tggattgtgg
     1081 ttattgcaac cctattagct ggtatatcga tgttgtttgc gcctcaacgc ggattgatag
     1141 cacaatggat agagcgttca aaaaacagaa ataaaacatt agaagagaat atcttgaaac
     1201 tgttttatca gataggagag aaggcgggag aatttagaaa gccagttttg gaggaagacc
     1261 taataaacta ccgtgcattt tccaatcaac aattgagaaa aggattgaaa ttgttgcttt
     1321 cgaagaaatt gttgacgatg aatttacatg cctggcaatt gacagaacaa ggtgttttag
     1381 aagcgcagcg aatcgttcgt ttgcatcgat tatgggaatt gtatttacag caatatctca
     1441 atctcgatcc tgatcatgtg catgatgatg ctgaggcaat ggagcatgtc atcaccccag
     1501 aaattgaagc acaattagat gagttattag gctatccagt tattgatcca cataatacct
     1561 taattccgcc acgggtatga cgttgaacga tttttggata gtattgacgg ctagtttaat
     1621 ctcactttgt tgtgttatac cgggaacttt tttggttttg cggcgcatgg ctatgtttgg
     1681 agatgcgatt tcacacgccg tattacccgg attggtgatt gcatatatga taagcggatc
     1741 gcgagcaagt atgtatatgt tgcccggagc agcagcaagt ggattactgg ttacatttct
     1801 aatcgaatgg gtgaataaac ggatgcggat tcagagtgat gccgccattg gactaattta
     1861 tactttcctt tttgccattg gagttattct tatttcggca tacacagcgc aaacagattt
     1921 agaccaagat tgtgtcttat atggtgaaat agcctacgtg ccttttgatt tactcataag
     1981 cgatgcagga aattctttag gtccac
//
LOCUS       DAOOQW010000315         1970 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_72241_length_1970_cov_5.524804, whole genome shotgun sequence.
ACCESSION   DAOOQW010000315 DAOOQW010000000
VERSION     DAOOQW010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1970
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72241_length_1970_cov_5.524804"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            282..1577
                     /locus_tag="PK281_11780"
     CDS             282..1577
                     /locus_tag="PK281_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOY50068.1"
                     /translation="MTPRRSFLKNLGISALSIPFLQQWNTVEAAELETHFESFSLDQA
                     ENDEDFWYRIQQSYSVSPNIINLNNGGVSPQPKVVQETFENFNRLSNEGPSYYMWRIL
                     DQGREPLRNSLAELAGCSADELAINRNATEALGTIIMGIPLEKGDEIVLSRWDYPNMI
                     NAWKWREKRDGIKLVWVEHPHTSENADELTKRYTDAFTSKTKVVHITHLINWNGQVIP
                     VAKIAQKAREKGILSISDSAHSFAHLDFKIPDLQVDFCGTSLHKWLCAPFGSGLLYVR
                     KDKIANLAPLFPGEKPESEDIRKFETLGTRSFPTELAIGRAIDFHNGIGSTRKFERLH
                     QLKTYWTEKVKTLPGVTVHTPASAQFSGAIGLFSIEGMKPSEIEPELFKPYGIHTVGI
                     DWNNISGVRVTPHVYTPFADLDVLVKAITTIAEKAQKKG"
     gene            complement(1589..>1970)
                     /locus_tag="PK281_11785"
     CDS             complement(1589..>1970)
                     /locus_tag="PK281_11785"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY50069.1"
                     /translation="STSSSGSISYSIGQVAYTAATGTTGSINQGVQQPYDIEIITGVE
                     HTEIEMTVFPNPALEQLQLTISTTNTENYSIQLFDAQGKLVLLNSKIQPINSIPVGDF
                     ISGIYFLSVFEHSTLVKSYRIVRP"
BASE COUNT          586 a          419 c          390 g          575 t
ORIGIN      
        1 tcgccttccc gattttacat cgggacgctc tagccaaatg agctaattcc cctttattac
       61 tatctcattc tggtcgcctt cccgatttta catcgggacg ctctagccaa atgagctaat
      121 tccccttttg atgagggttg caaaaatagg aaaatctata aggttttaag ttcattataa
      181 atttaagctc attgaaagca taatcttatt cattcctccc aactaagacc attttcttct
      241 ggatataatt ctatttttat aaaatactaa gtctctattt tatgactcct cgccgctcct
      301 ttcttaaaaa cctaggtatt tctgcacttt cgataccatt tctccaacaa tggaatacgg
      361 tcgaagcggc tgaattggaa acacatttcg aatccttttc tttagatcag gctgaaaatg
      421 acgaggattt ctggtatcga attcaacaat cgtattcggt ttctcccaat atcatcaacc
      481 tcaacaatgg tggtgttagt ccgcagccaa aggtggttca ggaaaccttc gagaacttca
      541 atcgactgag caacgaaggt ccatcgtatt acatgtggcg catattggat caaggtcgcg
      601 agccacttcg taattccctt gcagaattag cgggctgttc ggccgatgaa cttgcaatca
      661 accgaaatgc aaccgaggca ctgggaacta tcatcatggg aattccattg gaaaaaggtg
      721 atgaaattgt gcttagccgt tgggattatc ccaatatgat taatgcttgg aaatggcggg
      781 aaaaacgaga tggaatcaaa ctcgtttggg tggagcatcc tcatacttct gaaaatgccg
      841 atgagcttac aaaacgctac accgacgcat ttacgtccaa aaccaaagtc gtccatatta
      901 cgcacttgat caattggaac ggacaagtaa ttccggttgc gaaaattgct caaaaagccc
      961 gcgaaaaggg aatcttatcc atttcagata gtgcacattc ttttgcccat ctcgatttca
     1021 aaattcccga tttacaggtt gatttttgcg gaaccagttt acataaatgg ctttgtgcac
     1081 cgtttggctc aggtttactt tacgttcgaa aggataaaat tgccaatcta gcacctttgt
     1141 ttcccggtga aaaacccgaa agtgaagaca tccggaaatt cgaaaccctg ggcacacgtt
     1201 catttcctac cgaattagcg attggtcgtg ccatcgactt tcataatgga atcggcagta
     1261 cacgtaaatt cgagcgtttg catcaactca aaacatactg gacagagaaa gtcaaaacac
     1321 ttcccggagt aactgttcat acaccagcat cggctcaatt cagcggtgct attggattat
     1381 tctccatcga aggaatgaaa ccttccgaga tagaacctga gttgttcaaa ccctatggaa
     1441 ttcatacagt aggtattgat tggaataata tctcaggagt acgcgttacc ccgcatgttt
     1501 acacaccttt tgccgatttg gatgtgctcg tgaaagcaat tactacgatt gctgaaaagg
     1561 ctcagaagaa aggttgagtg attgctaatt atggtcgaac aattcgatac gatttcacca
     1621 gagttgaatg ctcaaaaaca cttaagaagt agattccaga aataaaatca ccaactggaa
     1681 tgctgttgat tggttgaatt ttactgttca acaaaacaag ttttccttga gcatcaaaga
     1741 gttgaatcga ataattctct gtattagtag tacttatggt taattgtagt tgctccaaag
     1801 caggattcgg aaacacagtc atttcaatct cggtatgttc aactcctgta attatttcaa
     1861 tatcataagg ctgctgaaca ccttgattta ttgaacctgt tgtgcctgtt gccgctgtgt
     1921 aagcaacttg gccaattgaa taggaaattg aacccgaaga agatgtgcta
//
LOCUS       DAOOQW010000316         1969 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_72312_length_1969_cov_4.703762, whole genome shotgun sequence.
ACCESSION   DAOOQW010000316 DAOOQW010000000
VERSION     DAOOQW010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72312_length_1969_cov_4.703762"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..628
                     /locus_tag="PK281_11790"
     CDS             <1..628
                     /locus_tag="PK281_11790"
                     /inference="COORDINATES: protein motif:HMM:NF026018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HOY50070.1"
                     /translation="VAHAWEKNDLENIRDIYYKSARYLFLLGGLLFLLVTLNAPFLFN
                     WLPDTYYQGVPVVAILSLGALFNLMTGSNTAIIFNSNRFTSGAIALVLVALLNLVLLY
                     VLIPQYGLIGAAWATCIASFTYNTFKFSFIYWRFKLQPFDKRTVSIVLAIIAAYGISS
                     ILPLSTHVLLNVFLHSLMACLVYGVIIWYSRAADDLKHMIPFLKKSKH"
     gene            complement(730..>1969)
                     /locus_tag="PK281_11795"
     CDS             complement(730..>1969)
                     /locus_tag="PK281_11795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY50071.1"
                     /translation="NSRKMPYIVRFDSRVSNTPDWHRYEFNIRPDLWYNFYDGVKFGL
                     HINGNYMNYKHVFDATFWLNTGLQKIKPGFDQDYSSFNPFSFKINYANPTDKILKGSS
                     VNASVRYLDGLAAAQLGFRIQNRKRNTSFYSYFKSMYRPDSSDVYYLINRTEWNPRQW
                     NNTVNVGLDHDYSYKRGNGRINLHMRTSGSGSDYDYSQLSLSVVNYNRLGKFNFNTRF
                     FAQIGTGTNLAPESALYLAGANPESLMDNKFTRSVGFFPQDWNGYGATTNHFQHGGGL
                     NLRGYAGYLAPYKRADGTSVLTYKSNSGVAVNAELEFDRLFPIRPRFTRNWLKINSYL
                     FADAGFIQSNLSNEKMAFASPRADAGLGLAFTIKKWGPLSMEKPLTFRLDMPFFLSRT
                     PDLSPDYVQYRWLVGVNRAF"
BASE COUNT          566 a          362 c          412 g          629 t
ORIGIN      
        1 agtggcgcat gcttgggaaa agaacgattt agagaacatt cgtgatatct actataaatc
       61 ggctcgatac ctctttctac taggaggatt attgttttta ctagtcacgt tgaatgcccc
      121 tttcctattt aattggttgc ctgataccta ttatcaagga gtaccggttg tggcaatatt
      181 gagtctagga gctttgttta atttgatgac aggttctaat actgctatta tttttaatag
      241 taatcggttt actagtggtg cgattgcatt ggttctcgta gcgcttttaa atcttgtttt
      301 actctatgta ctcattcccc aatatggatt aattggtgct gcttgggcta catgtatagc
      361 tagttttacc tacaatactt ttaagttctc gtttatttac tggcggttta agttgcaacc
      421 ttttgataag agaacagttt cgattgttct tgcaattatt gctgcttatg gcatttcttc
      481 cattttacct ttaagtactc atgtgctatt gaatgtattc ctacactcct taatggcttg
      541 tttggtttat ggagtaatta tttggtatag ccgtgctgcg gatgatttaa agcatatgat
      601 tccttttcta aagaaatcga agcattagat attacacgaa attgggcaag ggattgaagc
      661 aaggtgccgt gcaccgcgaa aagcccggtt tatgccccca aaaacaatta ctctgatttc
      721 ttttagcagt taaaaagcgc gatttactcc aactaaccaa cggtattgaa cataatctgg
      781 cgacaaatca ggtgttcggc tgaggaaaaa tggcatatct aatcggaagg ttaaaggttt
      841 ttccatgctt aatggccccc atttcttgat tgtaaatgca agccccaatc cagcatcggc
      901 acgaggtgag gcaaatgcca ttttctcgtt cgataaatta gactgaatga atcctgcatc
      961 ggcaaagaga tatgagttaa tttttaacca gttgcgggta aatcgtggac gaattgggaa
     1021 taagcgatcg aattctagtt cggcatttac agctacaccg gaattgcttt tataggttaa
     1081 cacactggtt ccatcggcac gtttataagg tgccaaataa cctgcataac cacgcaaatt
     1141 gagtccgcca ccatgttgaa agtgattggt agttgctccg taaccattcc aatcttgcgg
     1201 gaaaaatcca actgaacgag tgaatttatt atccatcaaa ctttcgggat tcgctccagc
     1261 gagatataaa gcagattctg gagctagatt cgttcccgta ccaatttgtg caaagaaacg
     1321 ggtgttgaaa ttgaacttac caagtcggtt ataattaact actgatagac tcaattgaga
     1381 ataatcataa tcggaacctg aaccagaagt tcgcatgtgc aagttaatac gtccatttcc
     1441 acgtttgtat gaataatcgt gatctaaacc aacattaacg gtattattcc actgacgcgg
     1501 attccattcg gtacgattaa ttaagtagta cacgtcggaa gaatcgggac gatacatact
     1561 cttgaaatag ctgtaaaagc tggtatttct tttccggttt tgaatacgga agcccaattg
     1621 tgcagcagct aaaccatcga gataacgtac agaagcattg actgatgagc ctttcaggat
     1681 tttatctgta ggattggcgt agttgatttt gaatgagaat ggattaaagc tgctgtaatc
     1741 ttgatcgaag cccggtttga ttttttgtaa acctgtattt aaccaaaatg tagcatcaaa
     1801 tacatgctta taattcatgt agtttccatt gatgtgtaat ccgaatttaa caccatcgta
     1861 gaaattgtac cataggtcgg gacgaatatt gaattcatat cgatgccaat cgggagtgtt
     1921 ggaaacgcgt gaatcgaagc gaacgatgta gggcatttta cgactgtta
//
LOCUS       DAOOQW010000317         1934 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_74372_length_1934_cov_3.766897, whole genome shotgun sequence.
ACCESSION   DAOOQW010000317 DAOOQW010000000
VERSION     DAOOQW010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1934
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74372_length_1934_cov_3.766897"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="PK281_11800"
     CDS             <1..308
                     /locus_tag="PK281_11800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY50072.1"
                     /translation="NHDNTLTADEQIHNLKMTIGSIREIEYDGDLKIEATMTAKEVET
                     AFWDTFGLDIQVFRKSGNAWLVTTSTDSRTLLEQNEIAREMNNPAENPELPDYQEHE"
     gene            complement(331..702)
                     /locus_tag="PK281_11805"
     CDS             complement(331..702)
                     /locus_tag="PK281_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020212924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HOY50073.1"
                     /translation="MSLKKACLVLVLIFSALFVDAQKDSLYKQTGMASYYAHKFHGRK
                     TASGEIFSKDSLTAAHKTLPFGTRIRVTNLKNNQSVIVRVNDRGMKGTNRIVDLSPAA
                     AKQIDMIRHGLLKVRIEELPK"
     gene            827..1852
                     /locus_tag="PK281_11810"
     CDS             827..1852
                     /locus_tag="PK281_11810"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOY50074.1"
                     /translation="MQQSPTLLLTGATGLLGSHALINLVKQGLQVRALVRPDSQYRER
                     LSEIFNLYKLDSSLLNSQVEFFEGDMLDLAALEDALIGIDSVIHCAASVRNTDKTEMN
                     NINIEGTSNLVNLCLDYSIKWFLHVSSVATLGPNPEGLVDEDYFFKFNPRLSNYALSK
                     YRAEQEVWRAIEEGLPAVIINPTYIIGASKSDTSSSAIFKYAKQGITFYTNGLGGYVD
                     VSDVAEVISKLYQKRISGKRYIVSAENWETFDFLKSVSQAMHSNIPKRAYHDWMGIPA
                     SIFFGLRKLFTGKTATFDRQVVRMAQSRNAYDNSRIVVETGIVFTPIRDSILASAERM
                     NRLLAEN"
BASE COUNT          619 a          342 c          385 g          588 t
ORIGIN      
        1 aaaaccacga taatacccta actgccgacg agcagataca caatcttaaa atgactattg
       61 gctcaattcg agaaatcgaa tatgatggtg atttaaagat tgaagcaaca atgactgcaa
      121 aagaagtcga aactgcattt tgggatacgt ttggacttga catacaagta tttcgtaaat
      181 caggtaatgc ttggttagta acaactagta ccgattcacg cacactttta gagcaaaatg
      241 aaattgctcg tgaaatgaat aatccggccg aaaatcccga gttgcccgat tatcaagagc
      301 acgaataaga ttcaacaagg gaatcaaagc ttatttagga agttcttcaa ttcgaacttt
      361 caataaacca tgtcgaatca tatcgatttg tttagctgca gctggactta aatctacgat
      421 tcgattagtt cctttcatgc ctcgatcatt tactcgtaca ataaccgact gattgttttt
      481 cagattggta actcgaatgc gtgttccaaa aggcaatgtt ttatgagcag ctgttaggct
      541 atcttttgag aatatttctc cgctggcagt tttacgtccg tgaaatttat gggcgtaata
      601 acttgccatt ccggtttgtt tgtataagct atctttttga gcatcaacga acaaagcaga
      661 gaatatcaac accaaaacca gacatgcttt cttcaagctc atagaatgtt ttttggtgaa
      721 aataaacaat ctgtaactaa tagtatttct gtaggacaaa ctctgcaaaa aactcctgct
      781 aattctggta tttagcagcg gaaattaagt agtttgcacg accatcatgc aacaatctcc
      841 tactctactt ttaacaggtg ctactggact tctaggaagt catgccttga ttaatttggt
      901 aaagcaagga ttacaggttc gtgctttagt ccgccctgat agtcaatatc gtgagcgcct
      961 aagtgagata ttcaacttat acaaactaga ttcatcactc ttaaattctc aggttgagtt
     1021 ttttgaagga gacatgttgg atttagctgc attagaagat gcattaattg gaattgattc
     1081 ggttatccat tgtgctgcaa gtgttcgaaa tacagataaa accgaaatga acaacatcaa
     1141 tattgaagga acttcaaact tggtaaacct atgtttggat tattctatca aatggttttt
     1201 acatgtaagt tcagttgcca cacttggacc gaatccagaa ggtttggtgg atgaagatta
     1261 tttcttcaag ttcaatcccc gattatccaa ttatgcctta tcgaagtatc gtgctgaaca
     1321 agaagtctgg cgagcgatag aagaaggatt gcctgctgtt ataattaatc caacttatat
     1381 tattggagca agtaaatctg atacgagtag cagtgccata tttaaatatg ctaaacaagg
     1441 aattactttt tatacaaatg gtttaggtgg atatgtggat gtttctgatg ttgcagaagt
     1501 tatatccaaa ctttaccaaa aaagaataag tggtaaacga tacattgttt ctgctgaaaa
     1561 ttgggaaacc ttcgattttc taaaatcagt ttcgcaggct atgcattcca atattcctaa
     1621 acgagcatat cacgattgga tgggaattcc ggcaagtata ttcttcggac tccggaaatt
     1681 atttacgggg aaaacagcca catttgatcg gcaagttgtc cggatggcac agagtagaaa
     1741 tgcttacgat aatagtagga ttgtagttga aacagggatt gtattcacac ctattcgaga
     1801 ttcgattcta gcttcagcag aacgtatgaa tcgtttgttg gcagagaatt agaagaatcc
     1861 ttgttgccat aggcttagta aatccatgct gatagcgatg ctaaagtgaa cacaaatacc
     1921 gggaatgatt gacc
//
LOCUS       DAOOQW010000318         1909 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_75842_length_1909_cov_4.996764, whole genome shotgun sequence.
ACCESSION   DAOOQW010000318 DAOOQW010000000
VERSION     DAOOQW010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75842_length_1909_cov_4.996764"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1328)
                     /locus_tag="PK281_11815"
     CDS             complement(<1..1328)
                     /locus_tag="PK281_11815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOY50075.1"
                     /translation="MRILPKFSVRIRLIIATVLVATVSFVSFSFKDDYFEISKNLDIF
                     ATLFREVNLYYVDEIQPGQMMKTGIDAMLESLDPYTNYIPEDDIEDARFMTTGQYGGI
                     GALIRTKDDFIVVSEPYEDAPAAKADLRAGDVILEIEGRSTKGKTSSDVVKVLKGQPN
                     TPVKLLIKREGEEKPLEKTIVREEIKVNNVPYYGMVDDKIGYIKLTGFTQDAGREVKD
                     ALLKLKENDKLEGVVLDLRGNPGGLLREAINIVNVFVDKNLDIVSTKGKVKDWDKTYK
                     SLNSPVDLNIPLAVLVNRSSASASEIVSGSIQDLDRGVIVGQRSFGKGLVQTSRPLSY
                     NTQLKITTSKYYIPSGRCIQAKDYSHRNEDGSVGTVPDSMIKEFTTRAGRKVYDGGGV
                     IPDVSMPVKEYTPISRSVMSNNIIFDFATQYRLKQKTSSKPNLETSTSSL"
     gene            complement(1343..1564)
                     /gene="yidD"
                     /locus_tag="PK281_11820"
     CDS             complement(1343..1564)
                     /gene="yidD"
                     /locus_tag="PK281_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009642598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOY50076.1"
                     /translation="MAQLLAKLVIALIRVYQLVISPVLPIACRYTPTCSVYGIEAVKK
                     YGPFKGTIMAIKRFLSCNPWGGHGHDPVP"
     gene            complement(1545..>1909)
                     /gene="rnpA"
                     /locus_tag="PK281_11825"
     CDS             complement(1545..>1909)
                     /gene="rnpA"
                     /locus_tag="PK281_11825"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HOY50077.1"
                     /translation="LSGRTTINEVFSLGKRFTIYPYRIIWRFRTPDKLPIIRFGITVP
                     KKISKKAVVRNRIKRCTREAYRTAKQDFIENWDAGQNPIDVFLVYTGNSETCTVELQE
                     KIILILKRLTATNGPTPR"
BASE COUNT          551 a          396 c          382 g          580 t
ORIGIN      
        1 ttcaatgagg atgtggatgt ttcaaggttt ggtttggatg aggttttttg ctttaagcgg
       61 tattgagtag caaaatcaaa gatgatatta ttgctcatca cactacgtga aatgggagtg
      121 tattctttca caggcatgct cacatcagga ataacaccac caccatcata cactttacga
      181 cctgcacgag tggtgaattc tttgatcatg gaatctggaa ccgttcccac gctaccatct
      241 tcatttcggt gagaataatc tttcgcttga atacagcgac cacttggaat gtaatattta
      301 gaagtggtga tttttagttg agtattgtat gagagaggac gtgaagtctg aaccaagcct
      361 tttccaaaac tacgttgacc aacaataaca cctctatcca aatcttgaat tgaaccgctt
      421 acaatttcag aagcagaagc cgaagaacgg ttaactaata ctgctaaagg aatgttaagg
      481 tcaacaggcg aattgagcga tttgtaggtt ttgtcccaat ctttcacttt gcctttggta
      541 ctaacgatat ccaaattttt atcgacgaat acattcacaa tgttgatggc ttcacgaagt
      601 aacccaccag gatttccacg taaatcgagg acaacacctt cgagtttatc attttctttc
      661 agctttaata aagcatcttt cacttctcga cctgcatctt gagtaaaacc agtgagtttg
      721 atgtacccaa ttttatcatc caccattccg taataaggaa cattgttcac tttaatttct
      781 tcacgaacaa tggttttttc gagaggtttc tcttcacctt cccgcttaat taagagttta
      841 acaggagtat ttggctgacc ttttagcact ttaaccacat ctgaactggt ttttcctttt
      901 gtgcttcggc cttcaatttc caaaatcaca tcacctgcac gtaaatctgc ttttgctgca
      961 ggagcatctt cataaggttc cgaaaccact atgaaatcgt ctttggtacg aatgagcgct
     1021 ccaattccac catattgtcc ggtagtcatg aaacgtgcgt cttcaatatc gtcttcggga
     1081 atgtaattgg tatacggatc aagcgattcg agcatagcat cgatgccagt tttcatcatc
     1141 tgtcccggtt gaatttcatc tacatagtag agatttactt ctcggaaaag cgtggcgaaa
     1201 atgtcgaggt tcttggagat ttcgaaataa tcgtccttga agctaaagct tacaaaagaa
     1261 acagtagcaa ccagaactgt tgcgataatt aggcggattc gaaccgaaaa cttaggtagg
     1321 attctcatgt ttaaatttta tgtcaaggta ccgggtcgtg gccgtgtcct ccccagggat
     1381 tgcaactcag aaacctctta attgccataa tagtaccttt aaaaggacca tattttttta
     1441 ccgcttcaat tccataaacc gaacaggtag gagtatagcg gcaagcaatt ggaagcaccg
     1501 gcgaaatcac cagctgatac acccgaatta gggcaattac cagcttagcg aggagttggg
     1561 ccattcgtgg ctgttaaacg tttcaaaatt aggattattt tctcctgaag ttcaaccgta
     1621 caggtttcag agtttccggt ataaacgaga aaaacatcaa tcgggttttg tccggcatcc
     1681 caattttcga taaaatcctg tttagcggtg cgataagctt ctcgagtaca gcgtttaatt
     1741 cgatttcgga caactgcttt tttcgaaatt tttttcggaa cagtaatgcc aaatcgaata
     1801 atgggaagct tatcgggagt tcgaaaacgc caaataatcc ggtagggata aatagtgaat
     1861 cgtttaccaa gactaaagac ttcgttgatt gtggttctac ccgacaatc
//
LOCUS       DAOOQW010000319         1883 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_77480_length_1883_cov_3.710066, whole genome shotgun sequence.
ACCESSION   DAOOQW010000319 DAOOQW010000000
VERSION     DAOOQW010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1883
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77480_length_1883_cov_3.710066"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="PK281_11830"
     CDS             <1..243
                     /locus_tag="PK281_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013622836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HOY50078.1"
                     /translation="VMKLGMAHPMGPLQLADFIGLDVCLSILRVLQDGFGNPKYAPSP
                     LLVNMVMANQLGVKSGVGFYDYSAGIKQARVADQFA"
     gene            complement(240..878)
                     /locus_tag="PK281_11835"
     CDS             complement(240..878)
                     /locus_tag="PK281_11835"
                     /inference="COORDINATES: protein motif:HMM:NF025492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4294 domain-containing protein"
                     /protein_id="HOY50079.1"
                     /translation="MRIISFSLIFLSFSILGRAQILLKERIKLVQDTPAQPTGFKVSY
                     IITDSGDTLPVVTLHPVSISEKRSFSSKREERKWSRLKRDVAKVYPYSKVAGKKLKEY
                     NDMMIGKSEREQKKMLRVAEEDLKKEFEGDIRNMTLNQGRILIKLIDRETGNTSYGLV
                     KDLRGSFQAFFWQSLARIFKTNLKSSYDPNNNAEDKMIEDIIESIEDGTFVN"
     gene            complement(904..1296)
                     /locus_tag="PK281_11840"
     CDS             complement(904..1296)
                     /locus_tag="PK281_11840"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOY50080.1"
                     /translation="MRYAETDQMGYVYYGQYATYFEVARVEAMKALGISYRSLEEAGT
                     MMPVTDYHIKYKKPALYDEEIRIQTDIQELPAFRIRFHYSSYNEKNELLNTAETELVF
                     IDKVRNRPVKAPELLLQALKPYFKSTGI"
     gene            1396..>1883
                     /locus_tag="PK281_11845"
     CDS             1396..>1883
                     /locus_tag="PK281_11845"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HOY50081.1"
                     /translation="MYTHKVIHTVIHALVFSLLTACSTTNQPHSVAKQNIIIGQGGAT
                     ASKQSIEEMIVPYRIPLQQTMAEVLVTSDAPAVKALPESSLGNLVCDLMLSQAGKITG
                     KQPDIAFYNTGGLRIELPAGDITVSMIYELAPFENELETIEILGKDLPILFEQISKRK
                     GGP"
BASE COUNT          543 a          372 c          376 g          592 t
ORIGIN      
        1 gttatgaaac tcggcatggc acatccaatg ggacctttgc aactagccga cttcattggc
       61 ttggatgtgt gtttatctat tttacgcgta ttgcaagatg gatttggtaa tccgaaatat
      121 gcaccatcac cactcttagt taatatggta atggcaaatc aattaggtgt gaaatcggga
      181 gttggttttt acgactattc cgcaggcatc aaacaagctc gcgtagctga tcagtttgct
      241 taattcacaa acgttccgtc ttcaatcgac tcgatgatat cttcaatcat cttatcctca
      301 gcgttattgt tgggatcgta agacgatttc aagttagttt tgaatatgcg tgcaagcgat
      361 tgccagaaaa atgcttgaaa cgaaccgcgt aagtctttta ctagtccgta agaagtattt
      421 ccggtttctc ggtcgatgag ttttataaga attcgtcctt gatttaaggt catgttacga
      481 atatcgcctt cgaattcttt tttcagatct tcctcggcaa ctcgtaacat tttcttttgt
      541 tcgcgctccg atttacctat catcatatcg ttgtattctt ttagcttttt accagcaact
      601 ttagaatacg gataaacttt ggcaacatct cgtttgagtc ggctccactt acgttcttcg
      661 cgtttactcg aaaaactccg tttctccgaa atgctaaccg gatgtaatgt aacaacaggc
      721 aaagtatcgc ctgagtcggt aataatatag gaaactttaa agccagttgg ctgagcagga
      781 gtatcttgta ctaatttgat tcgttctttc aacaagatct gagcacgacc caatatagaa
      841 aaggaaagaa aaatcaggga aaatgagatg atccgcatag tttgttttcg gaggcctgcc
      901 aatttatatg ccagttgatt taaaataagg ctttaatgcc tgaagcaata attcgggagc
      961 tttcaccgga cgattccgaa ctttatcgat aaataccaat tctgtttcgg cggtgttcag
     1021 tagttcattc ttttcgttgt acgaactata atgaaagcgg attcgaaaag caggtaattc
     1081 ttgtatatct gtttgaattc tgatttcctc atcatacaaa gcaggcttct tatacttaat
     1141 atggtaatct gtcactggca tcatcgttcc agcttcctct aacgatcgat aacttattcc
     1201 aagtgctttc atggcttcaa cacgtgcaac ttcaaagtag gtagcatact gtccgtagta
     1261 tacatatccc atctgatcgg tttctgcata acgcactcgc agaagggttt gatgtgtaaa
     1321 cacagtattt tattttttgg agttgacagg taaaggaaat gatgtaaatt ttcgggtttc
     1381 ttcttccctc ccgttttgta tacacataaa gttatccata cagttattca cgcactggtt
     1441 ttcagtttat tgacagcctg ttcgacaacc aatcaacccc attcggttgc gaaacagaac
     1501 atcataattg gacaaggtgg agctacagca agcaaacaat cgatagaaga aatgattgtt
     1561 ccctaccgaa ttccattaca gcaaaccatg gcagaagtgc ttgtaacgag tgatgcacca
     1621 gctgttaaag cgctccccga aagcagtttg gggaatttgg tttgtgattt aatgctcagt
     1681 caagcaggaa aaataactgg aaagcaacct gatattgcat tctataatac gggaggatta
     1741 cgaattgaac ttccggcagg tgatattact gtttctatga tatatgagtt agctccattc
     1801 gaaaacgagc tcgaaaccat tgaaattcta ggaaaagatt tacctatatt gtttgagcag
     1861 atatctaaaa gaaaaggagg acc
//
LOCUS       DAOOQW010000320         1821 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_81623_length_1821_cov_5.453567, whole genome shotgun sequence.
ACCESSION   DAOOQW010000320 DAOOQW010000000
VERSION     DAOOQW010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1821
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81623_length_1821_cov_5.453567"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="PK281_11850"
     CDS             <1..534
                     /locus_tag="PK281_11850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY50082.1"
                     /translation="RLNESGNFQVFRSPEKLSNQGISEAEMGEIQVYLDQCRLVCNKR
                     ENRVRQEKEQVIRRLGESYTQRVLLATNEQLSQLVLHKDELTKIKLNDRFELIRNFEP
                     AYHMPDAGGFAKGGFYSPYKRFGNLALEVRWANVLVIWLMTVVLSVMLYFDGLKKLLN
                     LPSYLRMLLKEKSSKGA"
     gene            complement(531..905)
                     /locus_tag="PK281_11855"
     CDS             complement(531..905)
                     /locus_tag="PK281_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HOY50083.1"
                     /translation="MDAIAIEMSPKTPLVNLSANGMMEFKGRSIPENSIEFYTPVFNW
                     IDKYSIKPLPKTSVHIQLEYFNTSSSKCLLDIFKKLQTLHQSGKSEVTVNWFYEEDDE
                     DMLEAGEDYQAIIKVPFVMTKI"
     gene            complement(909..1454)
                     /locus_tag="PK281_11860"
     CDS             complement(909..1454)
                     /locus_tag="PK281_11860"
                     /inference="COORDINATES: protein motif:HMM:NF038262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SiaB family protein kinase"
                     /protein_id="HOY50084.1"
                     /translation="MFDIYDFYEKMEDGKIMLSFKGEITSELLTSILHIMESKLDNIG
                     EEPRLRKKVYNVLVECLQNLYHHNDGLTDDPKANQRSALFLIGRSDNKTYKIITGNYI
                     LNTNVEAFRARIDKINAMSKDELKDYYIKTLNNEEFSGKGGGGLGLIDIARKSGQKLE
                     YSHTPIDDTYSFFSLLIKVIQ"
     gene            complement(1472..>1821)
                     /locus_tag="PK281_11865"
     CDS             complement(1472..>1821)
                     /locus_tag="PK281_11865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY50085.1"
                     /translation="NSQSRSALFRPGQTSKLSFTAFKGNEYRMTCGAENSLLNGGAIA
                     FKVMDAKSKKVIFDSTTDNAATFEFLCDNSLNLVLEITLPEAATAKESAKKVTYGCVG
                     FLLESRPALQTGF"
BASE COUNT          537 a          360 c          356 g          568 t
ORIGIN      
        1 cgattgaatg aatcgggaaa tttccaggtg tttagatcac ccgaaaaact aagcaatcaa
       61 ggtatttcgg aagctgaaat gggggagatt caagtttatc tggatcaatg tcgattagta
      121 tgtaataaac gggagaaccg cgttcgtcaa gaaaaggaac aggtaatccg cagactagga
      181 gaatcatata ctcagcgtgt tttacttgct acgaatgagc agttgagtca gttggtatta
      241 cacaaagacg aactcaccaa gattaagctg aacgatcgtt ttgaactcat ccgtaatttc
      301 gaaccagcct atcacatgcc cgatgcggga ggatttgcaa aaggtggatt ctattctccg
      361 tataaacgat ttgggaatct ggcactagaa gttcgctggg ctaacgttct ggtgatttgg
      421 ttgatgacgg tcgttttaag tgtaatgctc tatttcgatg gattgaaaaa gctgctcaac
      481 cttccctcct atttacgcat gctactaaaa gaaaaaagct ccaaaggagc ttagattttt
      541 gtcatgacaa aaggcacttt gatgatagcc tggtaatctt caccggcttc caacatgtct
      601 tcatcatctt cttcgtagaa ccaattaaca gtcacttccg attttcctga ttgatgcagc
      661 gtttgcaact ttttgaaaat atcgagcaga catttcgatg aactggtatt aaagtactca
      721 agctgaatgt gtacactcgt tttaggcaat ggcttgatgc tgtatttatc aatccagtta
      781 aaaacaggtg tataaaactc aatggaattt tctggtatag aacgtccctt aaattccatc
      841 attccattcg cgctaagatt taccagcgga gtttttggag acatttcaat tgcaatcgcg
      901 tccatttctt attgtattac tttaatcaac aaactgaaaa acgagtaagt atcatcaatt
      961 ggagtatgac tgtattctag cttctgtccc gatttacggg caatatctat cagtcctaat
     1021 cctccgccac ctttacccga aaactcctcg ttattgaggg ttttgatgta ataatcctta
     1081 agctcatctt tactcatcgc gttaatctta tcgattcgag cacgaaaagc ttccacgttg
     1141 gtattgagaa tataattccc agtaatgatc ttataggttt tattatcgct tctaccgatg
     1201 agaaataaag ccgaacgttg attggctttg ggatcatcag ttaatccatc gttatgatga
     1261 tagaggtttt gtaaacactc caccaataca ttgtagactt tctttcttaa tcgcggctct
     1321 tctccaatat tatcgagttt cgactccata atatgtaata tggatgtgag cagttcggac
     1381 gtgatttctc ccttaaaaga cagcatgatt ttgccgtcct ccattttttc gtagaaatcg
     1441 tatatatcga acatgttacg cgaagttccg attagaaacc tgtttgtaaa gctgggcgac
     1501 tttctaacaa aaagcccaca caaccataag taactttctt agccgattct ttagcagttg
     1561 cggcttcagg taatgtaatc tctaaaacaa gatttaaaga attgtcacac aagaattcaa
     1621 atgtggcagc attatctgtt gtgctatcga aaattacttt cttcgatttg gcatccatca
     1681 ctttgaaggc aattgcacca ccattcaaaa gagaattttc agcaccgcaa gtcatgcggt
     1741 attcgttgcc tttgaatgca gtaaaagaaa gtttagaagt ttgacctgga cggaacaagg
     1801 cacttctaga ttgtgagtta t
//
LOCUS       DAOOQW010000321         1793 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_83660_length_1793_cov_7.338895, whole genome shotgun sequence.
ACCESSION   DAOOQW010000321 DAOOQW010000000
VERSION     DAOOQW010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1793
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83660_length_1793_cov_7.338895"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..161
                     /locus_tag="PK281_11870"
     CDS             <1..161
                     /locus_tag="PK281_11870"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HOY50086.1"
                     /translation="LEKCKNNEQVMQVGIEWGIQQSKELIAAGVPCIHYYTMGKSEAV
                     KRIVKGAY"
     gene            complement(155..1660)
                     /locus_tag="PK281_11875"
     CDS             complement(155..1660)
                     /locus_tag="PK281_11875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY50087.1"
                     /translation="MKWIVWLLFLTCLKVGYSQQIQAGELSGIPRDFYPIRILGMVDG
                     NVLVLRANSETRTGFIKDIRVEVYDSLSLKKKTEWNFSGLFQDKQQFFPEDVRIWNGQ
                     VCIFGSAYDKQKKENQLQVIPVSSQGKLLDAKPLQKTKTSHFDFNHRRFRILGEPGQD
                     RLGVVSLVDSEEQGIPKVQVNLYDLNFNEKKQFESYLPFQGKKPEMVDWMIDSPGNLH
                     VLIRGNKSGDTLDRVFALYAFPVMSDEVIEYQLDIPGKIISGLDMSLNADDKLLVCGL
                     IRDDFQAKENVSGLFYLRIDRETGLVESKGIHRMDAGFKSLFAGENATTGLEDFSGFK
                     MREIVSGKGGNLLLVAENEDIHPECENDYRTGIEICHMHYRYGKILVVSFNQTGGVDW
                     YQTLAKAQHSIDDEGGFLSFIQSKSSTGNLEFIYNGAIGKTGRADEVMENPEKSQLQM
                     VSVNSNGQLSEPNLASSYPVMPAVSWIERKGSILTLSQDNGSARLVRFSNQ"
BASE COUNT          545 a          418 c          288 g          542 t
ORIGIN      
        1 aactcgaaaa gtgtaagaat aacgaacaag tgatgcaggt aggaattgaa tggggaatac
       61 aacaatctaa agagcttatt gccgcaggtg ttccttgtat tcactattac accatgggaa
      121 aatcagaagc cgtaaaacgt attgtaaaag gcgcttattg attgctaaat cgaactaatc
      181 gagcactgcc attatcttga gataaagtta gaattgaacc ttttcgttca atccatgaaa
      241 cggccggcat tacagggtaa cttgaagcta aattaggttc tgataattgt ccgttgctat
      301 ttacggagac catttgtaat tggctctttt cgggattttc cattacttca tctgccctgc
      361 ccgttttccc aattgctccg ttatagataa actccaaatt tcctgtggag gatttagatt
      421 gtataaatga aagaaatcct ccttcatcat caatcgaatg ctgtgcttta gccaaggttt
      481 gataccaatc cactccgcca gtttgattga atgaaactac tagaatcttc ccatagcgat
      541 aatgcatgtg gcatatctcg attccagttc ggtaatcgtt ttcgcattcg ggatgaatgt
      601 cctcattttc ggccactagc aataaattcc cgcctttgcc agaaacaatt tctctcattt
      661 taaatccaga aaaatcttct aatccagttg ttgcattttc accagcaaat aaggacttaa
      721 atccagcatc catccggtga atccctttgc tttcaaccaa tccagtttct cgatcaattc
      781 gtaggtaaaa caatcccgaa acattctcct tcgcctgaaa atcgtcgcga attaatccac
      841 agaccaataa tttatcatca gcatttaacg acatatccaa tcccgaaata atcttacccg
      901 gaatatctaa ctggtattcg ataacctcat cactcatcac aggaaaagca tacaaagcaa
      961 aaactcgatc taaagtatcg cccgatttat tgccccgaat caatacatgc aaattacctg
     1021 gtgagtcaat catccaatca accatttcgg gcttctttcc ttgaaatggc agatacgatt
     1081 caaactgttt cttttcatta aaattcaggt cgtataaatt cacctgaacc tttggaattc
     1141 cttgttcctc tgaatcaact aaactaacaa cgcccaaacg atcttgtccc ggttctccta
     1201 agattcgaaa tcgacgatga ttaaaatcga aatggctcgt ctttgttttt tgaagtggtt
     1261 ttgcatctag caatttacct tggctagaaa caggaattac ttgtaattga ttttccttct
     1321 tttgcttatc gtatgcagat ccaaaaatgc aaacctgtcc gttccaaatc cgaacatcct
     1381 ccggaaaaaa ctgctgttta tcttgaaata agccgctgaa attccattcg gttttcttct
     1441 ttaatgaaag cgaatcgtac acttcaactc gaatgtcttt aatgaatccg gtgcgtgttt
     1501 cagaattagc acgaagtaca agtacattac catctaccat ccctaaaatt cggataggat
     1561 aaaaatcgcg gggaattccg cttagctcac ctgcttgaat ttgctgagaa taaccaactt
     1621 tcaaacaagt caaaaacaga agccaaacta tccacttcat gcgctagcct tcttcaaaac
     1681 atttccagca gatgtgttct tattcgccaa ctgaccacaa gctgcatcta tatctttccc
     1741 cctacttctc cgcacattca caatgactcc tttttgctcc aaaaacgtag tga
//
LOCUS       DAOOQW010000322         1717 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_89616_length_1717_cov_3.566185, whole genome shotgun sequence.
ACCESSION   DAOOQW010000322 DAOOQW010000000
VERSION     DAOOQW010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89616_length_1717_cov_3.566185"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /gene="rimM"
                     /locus_tag="PK281_11880"
     CDS             <1..327
                     /gene="rimM"
                     /locus_tag="PK281_11880"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HOY50088.1"
                     /translation="EDVDNAEQADSLTGCSVFLPLEKLPPLSGTKFYYHEVIGWKIED
                     LTSGLSAGIIKDVLENGPNDLFQLTNEGQEVLIPVADDWIVKVDRELGVISMNLPEGL
                     LEVNRK"
     gene            345..1082
                     /locus_tag="PK281_11885"
     CDS             345..1082
                     /locus_tag="PK281_11885"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOY50089.1"
                     /translation="MSNSWFQFKQFKVEQDKCAMKVGTDGVLLGAWAPLDNVRSVVDL
                     GSGTGLISLMIAQRSKARITGIESDMDAATQSAENFVHSPWPERLESVWADVHEMSTL
                     WAGQFDLAVCNPPFFQGHVRPSNAPRNQARHLLSPKTEIKKTWFEAAYKITKETGRAA
                     FIFPYEFAHEWLSEAESAGWYVQIRVNVRGHAGKEYKRVLVYLVKHPVAIQEEELCIE
                     SEERSVYTAAFGQLVSDFYLDARIETV"
     gene            1184..>1717
                     /locus_tag="PK281_11890"
     CDS             1184..>1717
                     /locus_tag="PK281_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOY50090.1"
                     /translation="MNYDLIVIGSGPGGYVAAIRASQLGLKVAVVEREELGGICLNWG
                     CIPTKALLKSAQVFEYLQHAADYGIKLNGEAEADFESMVKRSRGVADGMSKGIQFLLK
                     KNKIDTIIGFGKITKDKKVEVKGVDGKTTQYTAKSIVLATGARSRELPNLKQDGKKII
                     GYRQAMTLPKQPKSMVVV"
BASE COUNT          534 a          318 c          405 g          460 t
ORIGIN      
        1 gaagatgtcg ataatgccga acaagcagat tctcttaccg gctgttcggt ttttcttccg
       61 ctcgaaaaac ttcctcctct tagcggaact aaattctatt accacgaagt gattggctgg
      121 aaaatagaag atcttacttc aggtttatct gctggtatca ttaaagatgt ccttgaaaat
      181 ggtccaaacg acctgtttca acttacaaac gaaggacaag aagttctcat tccagttgca
      241 gacgactgga ttgtgaaagt agatcgggaa ttaggtgtga tttcgatgaa tctacctgaa
      301 ggtttattgg aagtaaacag gaaataaaac tggccgacaa tcacatgtct aacagctggt
      361 ttcaattcaa acaatttaaa gtcgagcaag ataaatgtgc catgaaagtg ggcaccgacg
      421 gcgtattgct tggtgcttgg gctccacttg ataatgttcg atcagttgtt gatttaggtt
      481 ctggaactgg actcatatca cttatgattg ctcagcgatc gaaagctcga attactggaa
      541 tcgaatcgga tatggatgca gcaacgcaat ctgccgaaaa tttcgtgcat tctccttggc
      601 cagaacgtct cgaaagtgtt tgggctgatg tgcatgaaat gagcacttta tgggctggac
      661 agtttgattt agccgtgtgt aatcctccct tttttcaagg acatgttcgt ccgtcgaatg
      721 ctcctcgaaa tcaagcaaga catttattaa gtcccaagac agagattaag aaaacttggt
      781 ttgaagcggc ttacaaaatc acaaaagaaa cgggacgggc ggcgtttatc tttccttatg
      841 aatttgcaca tgaatggctc agcgaggcag aatcggcagg ttggtatgtt caaattcgag
      901 tgaatgtgcg aggacatgct ggaaaagaat acaaacgagt tttggtttat ttggtgaagc
      961 atcctgttgc tattcaggag gaagaattgt gtatcgaaag tgaagaacgt tcggtgtata
     1021 ccgctgcatt tgggcaattg gtgagcgatt tctatttaga tgcacgaatt gaaacagtct
     1081 gaaaaacctg ttcagaaccc gataaagaaa cgcggagaat acatgatttt cgcttaattt
     1141 cgccgccgat ccggaacaat ccggagctaa gcatatattc cgaatgaact acgatcttat
     1201 tgtaataggt agcggcccgg gaggctatgt agctgccatc cgtgcatccc aattgggact
     1261 aaaagtagcg gttgttgaac gtgaagaact aggtggaatc tgcctcaatt ggggatgtat
     1321 tccaacgaaa gcactattga agagtgctca agtgtttgaa tatctgcaac atgctgctga
     1381 ttatggaatc aaattaaacg gtgaagccga agctgatttc gaaagcatgg ttaaacgtag
     1441 ccgtggcgtt gccgatggaa tgagtaaagg catccaattc ctcctgaaga aaaataaaat
     1501 cgataccatc atcggatttg gaaaaattac caaagacaaa aaagtagaag taaaaggagt
     1561 tgatggaaaa acaactcaat acacagctaa aagcatcgta cttgctacag gtgctcgcag
     1621 tcgagaattg ccgaatttga aacaagacgg taaaaaaatc ataggttatc gtcaagcaat
     1681 gactttacct aaacaaccta aatcgatggt tgtagtt
//
LOCUS       DAOOQW010000323         1677 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_93073_length_1677_cov_6.102959, whole genome shotgun sequence.
ACCESSION   DAOOQW010000323 DAOOQW010000000
VERSION     DAOOQW010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93073_length_1677_cov_6.102959"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1316
                     /locus_tag="PK281_11895"
     CDS             <1..1316
                     /locus_tag="PK281_11895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY50091.1"
                     /translation="LLSALVWTSISIAQMDPSYGQGGTLKLNEDGSHYLKFTLTNQVW
                     ARYNESNPGTTVEGKPKSETFDIGIRRSRIQMYGAINSRSFIYVQLGMNNFNHLSDRK
                     VGLFFHDAIAEYSVVKNKLSIGGGLTNWVGLSRLSNASIVTFMPMDSPIFPFATIDAT
                     DQFNRKLSLYAKGKLGKLDYRLALTDPMNIEKSSLYDPAKTISTNSQFATGAANKQFQ
                     GYLMWQFLDQESNLTPYTVGSYLGSKKVFNVGAGYIFQHDALWRRTANSDTVHTDMTL
                     LGADVFLDMPLRKEAGDAITAYASVYSNNYGSGYLRNNGIMNPATGTVAGQASFNGPG
                     NAFPLFGTGTVYYTQIGYLLGKGWTKPEKLRIQPYFCYMLGDYDRLNKPMQMTNAGIN
                     FFLNNHNTKITLDWQQRPVYLNAVTGPLPFSGRKNCVTLQFQVAI"
     gene            1339..>1677
                     /locus_tag="PK281_11900"
     CDS             1339..>1677
                     /locus_tag="PK281_11900"
                     /inference="COORDINATES: protein motif:HMM:NF021578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na-translocating system protein MpsB"
                     /protein_id="HOY50092.1"
                     /translation="MKKEYSSFDEHRVLHDLKHYLPAQAPLKDFIHHNTLHAFQDRKF
                     HTAIRSASEMFGYRVSLKLEEYRELYSQNRVREDVLEHVIARRMGIANVAKWKDRMLN
                     KEYDTTTSPRI"
BASE COUNT          499 a          309 c          367 g          502 t
ORIGIN      
        1 ttctactgtc tgctttagtg tggacaagta tcagtattgc tcaaatggat ccaagctatg
       61 gacagggcgg tacgcttaaa ttaaatgagg atggaagcca ttacttgaaa tttacactta
      121 ccaatcaggt atgggctcga tataatgaaa gtaatcccgg tactacggtc gagggaaaac
      181 caaaaagtga aacgttcgat attggtattc gaagaagccg aattcagatg tatggagcta
      241 tcaacagtcg ttcttttatc tacgttcagt tgggtatgaa taatttcaat cacttgagcg
      301 accgaaaagt gggtttgttt ttccatgatg ccattgccga gtattcggtt gtaaaaaaca
      361 aattgagcat tggtggagga ttgaccaatt gggttggact ttcgcgcttg tcgaatgcta
      421 gtattgtgac gtttatgcca atggatagtc cgattttccc attcgctaca atcgatgcaa
      481 cggatcaatt caaccggaaa ttaagcttgt atgcaaaagg aaagctcgga aaacttgatt
      541 atcgacttgc cttgaccgat ccgatgaata tcgaaaaatc gagtttgtat gatcctgcca
      601 aaaccatctc tacgaattct caatttgcta caggagctgc gaataaacaa tttcaaggct
      661 atttaatgtg gcagtttttg gatcaagaaa gtaacctaac tccctacacg gttggtagtt
      721 atttaggaag caaaaaggtt tttaacgttg gagctggtta tatttttcag cacgatgctt
      781 tgtggcgcag aactgctaat tccgatacag ttcatacgga tatgactttg ttgggagctg
      841 atgtattcct cgatatgcct ttgcgtaaag aagctggaga tgcaattaca gcttatgctt
      901 cggtttatag taataattat ggttcgggtt atttaaggaa taatggaatc atgaatcctg
      961 caacaggaac ggtagctgga caagcttcct ttaacggtcc ggggaacgca tttcctttat
     1021 ttggaacggg aacagtttac tatactcaaa ttgggtatct gttaggaaaa ggctggacca
     1081 agcctgaaaa gcttcgtatt caaccttatt tttgttacat gttaggagat tatgatcgac
     1141 tcaacaaacc gatgcaaatg accaatgcgg gtattaattt cttcctcaat aaccataaca
     1201 cgaaaattac actcgattgg caacaaaggc cagtttatct taatgctgta acaggtccat
     1261 taccctttag cggcagaaag aattgtgtaa cacttcagtt tcaggtggct atttgattca
     1321 tccgttgaaa tcatgattat gaaaaaagaa tattcttcgt tcgatgagca ccgagtgcta
     1381 cacgatttga agcattattt gccagctcag gctcctttaa aggactttat ccatcacaat
     1441 acattacacg catttcaaga ccggaaattc cataccgcca ttcgtagtgc ttcggaaatg
     1501 tttggttatc gggtttcgtt gaaattagag gagtaccgcg agttatacag tcagaataga
     1561 gttcgtgaag atgtgcttga acatgtcatt gctcgtcgaa tgggcattgc taatgtagca
     1621 aaatggaaag atcgtatgct caacaaagag tacgatacaa ccacttctcc tcgtatt
//
LOCUS       DAOOQW010000324         1655 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_95166_length_1655_cov_3.860000, whole genome shotgun sequence.
ACCESSION   DAOOQW010000324 DAOOQW010000000
VERSION     DAOOQW010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95166_length_1655_cov_3.860000"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            42..656
                     /locus_tag="PK281_11905"
     CDS             42..656
                     /locus_tag="PK281_11905"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOY50093.1"
                     /translation="MYLSKYCFSIFLFFSVLTSQAQTTALDWYNQGTAKGLAEEYSEA
                     IDCFNNALKLQPNFEDALYNRGLCLFRKKQYKTALIDFNSIIEKNPESARSYNLRGQI
                     KSALGDHKGALVDFNDAITLVNNDPSFYYNRALSSYSLGTYAEAIADLNVCLNLESKN
                     AKAYYKRGIIQYVSGSKEAGCIDLGKSADLGYPEAFDIIRIYCR"
     gene            671..1588
                     /locus_tag="PK281_11910"
     CDS             671..1588
                     /locus_tag="PK281_11910"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOY50094.1"
                     /translation="MKKKRSRISGIFSYPLLGLIYAVSVLPFPVLYFISDGIYLLIYR
                     LAGYRVQVTRKNLKTALPNLTEKELLLVEKRFYHHLCDVIVETVKSFTISPKQLKKRM
                     RLLNPELLAHYHQHGRKMIAVTGHYGNWEWAAITLPLQSDYVPQGVYLSIKDPVMNRA
                     MIRSRSRFGINLLEAGKIFQELENRKDQLSITGFVADQSPSKPEKGVWLEFLHQETVF
                     AKGTEKYAALYDMAVAFGKIRKIKRGYYTLEYEVVTENAALNPEGDVTVKHAAILERT
                     IQEQPEFWLWSHKRWKHRRPEDARIFGRS"
BASE COUNT          527 a          330 c          321 g          477 t
ORIGIN      
        1 cggacgaaaa gtaaaacctg tagaacctaa aaaagcaccg ggtgtattta agtaaatatt
       61 gtttttccat tttcctcttt ttctctgtac taacaagcca agctcaaaca acagcgctcg
      121 attggtacaa tcaaggtact gcaaaaggct tagctgaaga gtattccgag gcaattgatt
      181 gctttaacaa cgcattaaaa cttcaaccca attttgaaga tgcgttatat aatcgtggct
      241 tatgtttatt tcgcaagaag cagtataaaa ctgcattaat cgatttcaat tcaatcattg
      301 aaaagaaccc tgaaagtgct cgttcctata atcttcgtgg tcaaataaag agtgcactag
      361 gtgatcataa aggcgcgctt gtcgatttta atgatgcgat tactttagta aataatgacc
      421 cttcgtttta ctacaaccgt gcgctttcta gctattcttt gggaacatat gcagaagcca
      481 tagctgactt aaatgtttgt ttaaacttgg aatctaaaaa tgcaaaagct tattacaaaa
      541 gaggaatcat tcaatatgtt tctggtagta aagaagctgg ttgcatcgat ttaggtaaat
      601 cggccgattt gggatatcca gaagctttcg atattatccg catttattgc cggtaagatt
      661 ttcccgtaag atgaagaaga aaaggagccg gatttcggga atattctcat accctttatt
      721 aggattgatt tatgccgtat cggtgttacc ctttcctgtt ctttacttca tttcggatgg
      781 aatttactta ttgatttatc ggttagcggg atatagagta caagtcaccc gaaaaaatct
      841 taaaactgct cttcctaacc ttactgaaaa agaattactc ctagtcgaga aacgcttcta
      901 tcaccacttg tgcgatgtaa ttgttgagac ggtgaaatct ttcaccatat ctcctaaaca
      961 gctcaaaaaa cgtatgcgac ttctaaatcc tgagttgctt gctcactatc atcaacatgg
     1021 acgtaaaatg atagctgtaa ctggacatta tggtaattgg gaatgggctg caatcacact
     1081 tccattacaa agcgactatg ttcctcaagg tgtgtattta tcgattaaag atccagtcat
     1141 gaatcgagct atgatacgct cgagaagccg tttcggtatt aacctgcttg aagccggtaa
     1201 aattttccaa gaactcgaaa atcgtaaaga tcaactaagt attaccggat ttgtagccga
     1261 tcaaagtcct tctaaacccg aaaaaggtgt ttggctcgaa ttccttcatc aagaaacagt
     1321 tttcgcaaaa ggcacagaga aatatgcagc actgtatgac atggcagttg cttttggaaa
     1381 aatcagaaag attaaacgag gctactacac acttgaatac gaagtcgtta ccgaaaacgc
     1441 agcactaaac cctgaaggtg atgttactgt caagcatgcc gcaattctag aacgtactat
     1501 tcaagaacaa cccgaattct ggctttggtc acataagcgc tggaaacacc gccgtccaga
     1561 agatgctcgt atatttggac gatcatgaac gccaacgaac cacttgcaga aagaatgcgt
     1621 ccttcgacct tagaaggata cgttggacaa actca
//
LOCUS       DAOOQW010000325         1646 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_96045_length_1646_cov_3.033312, whole genome shotgun sequence.
ACCESSION   DAOOQW010000325 DAOOQW010000000
VERSION     DAOOQW010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96045_length_1646_cov_3.033312"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..918)
                     /locus_tag="PK281_11915"
     CDS             complement(<1..918)
                     /locus_tag="PK281_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007842280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOY50095.1"
                     /translation="METLESTQKLVARPHFKSRYDNFIGGKFVAPVNGEYFDNISPID
                     GLPFTQAARSTKEDIELAIEAAWRAFPSWSKTSVTDRSNMLLKIADVMEKNLELLATI
                     ETIDNGKAIRETRAADLPLAIDHFRYYAGVIRGEESTVSELDHNTLSLNIHEPIGVVG
                     QIIPWNFPLLMAAWKIAPALAAGCCTIVKPAEQTPTSLIVLMELIAGILPDGVLNVVT
                     GFGPEAGKPLAQHPRLSKVAFTGETTTGRLIMQYASENLIPVTMELGGKSPNIFFPSI
                     VDADDEFFDKAIEGAVLFALNQGEVCTCPS"
     gene            1081..>1646
                     /locus_tag="PK281_11920"
     CDS             1081..>1646
                     /locus_tag="PK281_11920"
                     /inference="COORDINATES: protein motif:HMM:NF018431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOY50096.1"
                     /translation="MQPSFTPFAPALRKPEDLITMVENRTVISRPQFELNVFETHLQA
                     DNVRLQFDDLVFTSMLRGKKVMHLKGDNHFEYYPGESVILGPGEEMLIDFPEAKVSNP
                     TQCLALAVNQDIIRQTITLLNENYPKSEDGEIWDIAWNQFHFRNTDQLADTLSRLVKL
                     SFEPHQSRDLLANLSIQELIVRLLQTQAR"
BASE COUNT          502 a          329 c          319 g          496 t
ORIGIN      
        1 tgaaggacaa gtacacactt caccttgatt caaggcaaat aataccgcac cttcaatggc
       61 tttgtcgaag aattcatcat cggcatctac aatacttggg aagaaaatgt taggagattt
      121 tccgcctaat tccatcgtta caggaatcaa gttttcggaa gcatactgca taatcaaacg
      181 tccagtagtt gtttctcctg tgaaagcaac tttactcaaa cgtggatgtt gagcaagtgg
      241 ttttccagct tccggaccga aacctgttac cacgttcaaa acaccatccg gtaaaatacc
      301 agcaataagt tccattaaca cgatcaaact tgttggcgtt tgttcagctg gttttacaat
      361 ggtacagcat cctgcagcca atgctggtgc gattttccat gcagccatca atagaggaaa
      421 gttccaagga atgatttgtc caacaacacc aattggctcg tggatattca aagacaaagt
      481 gttgtgatca agttcagaaa cggtactttc ttcaccgcga ataacaccag cataatagcg
      541 gaaatggtca attgccaatg gaagatccgc tgcgcgtgtt tcacgaattg ctttaccatt
      601 atcgatggtt tcgatagttg ctaaaagctc tagattcttt tccatcacat cagcaatctt
      661 caacaacatg ttactacgat cagtaacgga tgttttcgac caagatggaa atgctctcca
      721 agctgcttca atcgctaatt caatatcttc ttttgttgaa cgtgctgcct gtgtaaaagg
      781 caatccatcg ataggtgaaa tgttatcgaa atattctcca ttaactggtg cgacaaactt
      841 gccaccaata aagttgtcgt aacgactttt gaaatgtggt ctagccacta atttttgggt
      901 tgattcaagt gtttccataa gagtgatgat tcgttttaac aagattaacc ttaggaaatt
      961 ggactatgct tgagtattgt atcaaaggac tttacaatcc tctcattgtg tatttttaac
     1021 gtcaacaaac cgcttacaat tattaaattg ctttccgtat cacaaactac ctacgtatac
     1081 atgcaaccat cattcactcc atttgcacca gcacttcgta agcccgaaga tttgattaca
     1141 atggttgaaa accggacggt tatttctcga ccacaatttg aattaaatgt gtttgaaaca
     1201 catcttcaag cagataatgt tcgattgcag tttgatgatt tagttttcac ctcgatgttg
     1261 agagggaaaa aagtgatgca tttaaaagga gataatcatt ttgaatatta ccctggtgag
     1321 tcggtgattt taggtcccgg agaagaaatg ttgattgatt tccccgaagc aaaagtgagt
     1381 aatccaactc aatgcttggc tttggctgtt aatcaggata ttattcgtca aaccattacc
     1441 ttattgaatg aaaattatcc taaatcggaa gatggagaga tatgggatat tgcttggaat
     1501 cagtttcatt ttagaaatac ggatcaatta gccgatacgc tgagtcgatt ggttaaattg
     1561 agctttgagc cacatcaatc gcgcgattta ctagcaaatt tgtcgataca ggaattgatc
     1621 gttcgactgc tacaaacaca agcacg
//
LOCUS       DAOOQW010000326         1609 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_99578_length_1609_cov_3.646718, whole genome shotgun sequence.
ACCESSION   DAOOQW010000326 DAOOQW010000000
VERSION     DAOOQW010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99578_length_1609_cov_3.646718"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..823)
                     /locus_tag="PK281_11925"
     CDS             complement(<1..823)
                     /locus_tag="PK281_11925"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOY50097.1"
                     /translation="MNRYHPILRFRALLCLILLLSACTEKRDLKQNTVVVHFLAEPSG
                     LHPTNDNNAYQRFVFQCTQKRLMSIDLRDQKLHPDVLASYPEIQPDSLSYRCKIRSDI
                     RWDDGNPMSVDDILFTFKVITCPLVNNPDGKSYFNKLIAADAIEGTKDEFTLRLSKRY
                     YDNLSLLSYVIVLQEKFYDPAGLLRAYSVSDFQEKKELMTSDSTLQAFAAKFNAADYG
                     RVPALLNGLGPYAVSEWNTGSSIVLKRKTNWWGVNSNSPYEKAFPEKIIFQIIKDM"
     gene            complement(888..1262)
                     /locus_tag="PK281_11930"
     CDS             complement(888..1262)
                     /locus_tag="PK281_11930"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HOY50098.1"
                     /translation="MMSSVDRYIESRPNSEQAVFFCTRRLILESAPGMMEKISYKIPF
                     YYYLGPFCYLNPYKGGLDLGFFYGLDLSNEQGILESRDRKQVQSIPIYTLDDYYEKEL
                     ALREILQEALLVSQIRHSKKKP"
     gene            complement(1263..>1609)
                     /locus_tag="PK281_11935"
     CDS             complement(1263..>1609)
                     /locus_tag="PK281_11935"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY50099.1"
                     /translation="NTYDVELIVKNDCGSDTIQTTINVIKIVGIEDVNANQLKFYPNP
                     VQDQISIHNPWKNNQLVQVNMYDSVGKLVLKNTSNDSLIQLSLKDLAPGIYYLQLVQG
                     QTILKSRFVHTR"
BASE COUNT          481 a          324 c          316 g          488 t
ORIGIN      
        1 ccatgtcttt gataatttga aagatgattt tttcggggaa tgctttctcg tagggtgaat
       61 tactattcac tccccaccag tttgtcttac gttttagaac gatggaagag ccagtattcc
      121 attcactaac ggcataaggt ccaagtccgt tgagtaaagc tggaactcgt ccgtaatcag
      181 ctgcattgaa cttggcggca aatgcttgta aggtagaatc gctcgtcata agttccttct
      241 tttcctgaaa atcgctcacc gaataggcgc gtaataaacc agcaggatcg tagaactttt
      301 cttgtagaac tattacatag ctcaacaaac tgaggttatc gtagtaacgt ttactaagtc
      361 ggagtgtaaa ctcgtcctta gtgccttcga tggcatcagc agctatgagt ttattgaagt
      421 aagatttccc atctgggtta tttactaagg gacaggtaat gactttgaat gtaaagagta
      481 tatcatctac actcattgga tttccatcgt cccaacgaat atcggaacgg attttacagc
      541 ggtacgaaag actatcaggt tgaatttcgg ggtaggaggc aagcacatcg ggatgaagtt
      601 tttgatccct taaatcgatg gacatcaacc ttttttgcgt gcactgaaaa acaaatcgct
      661 gataggcgtt attgtcgttt gtgggatgta atccactcgg ctcagctaga aaatgaacca
      721 cgactgtatt ctgcttcaga tctcgcttct cggtacaggc cgacaaaagc aaaatcagac
      781 ataataaagc ccgaaaacgc aggattgggt gatagcgatt catttgcttc aaacataccc
      841 caaaatcgct caggaaaaca aactacggcg gtgattttga cgtgttttta tggttttttt
      901 ttcgagtgac gaatttgact aaccagcaaa gcttcttgaa ggatttcacg aagggctagt
      961 tctttctcat aataatcatc taacgtgtaa ataggaatac tctgaacctg ctttctatcg
     1021 cgactttcca agattccttg ttcgttagat aaatctaacc cataaaagaa acccaagtct
     1081 aaaccacctt tgtagggatt taggtagcaa aaaggtccca gataatagta aaacggaatc
     1141 ttatacgata tcttttccat catacccgga gccgactcta atatcaacct tcgagtacag
     1201 aaaaacacgg cttgctccga attgggtctg ctttcgatat agcggtcaac gctactcatc
     1261 atttatcggg tatgaacaaa acgggatttc aaaatcgttt gtccttgaac aagctgcagg
     1321 taatatattc ccggagccaa atctttcaac gacaattgaa ttaaggaatc gttcgaagtg
     1381 tttttcaaaa ccaatttgcc caccgaatca tacatattta cctgaaccaa ttgattattt
     1441 ttccatggat tatgaatgga aatttgatct tgaacagggt tcgggtaaaa ctttagttga
     1501 ttagcgttaa catcctctat accaacaatt ttaataacat taattgttgt ttgaatcgta
     1561 tcacttccac agtcgttttt aacgattaat tctacatcgt aagtattgt
//
LOCUS       DAOOQW010000327         1420 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_122181_length_1420_cov_3.715018, whole genome shotgun
            sequence.
ACCESSION   DAOOQW010000327 DAOOQW010000000
VERSION     DAOOQW010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1420
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122181_length_1420_cov_3.715018"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /locus_tag="PK281_11940"
     CDS             complement(<1..637)
                     /locus_tag="PK281_11940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY50100.1"
                     /translation="MRNQPLRIFCLWILVFLSSLAQAQTTSSCFRIESILVDACGNDE
                     GQNEMVRFLVGPNALNTADLNVAWATSGNSWDGVCQNASTSAKVAAMNATIEHCGLLK
                     EPTGGVLPANSKVILISGINFNPALNSFAGLADTLYVIFHCSTEIIGNFANVGSGIRN
                     LSMTFNSPSSCTQTVSYDRSLLLGGDGALAEYTQTGVCSYGNNGCTAPYIVP"
     gene            complement(729..>1420)
                     /locus_tag="PK281_11945"
     CDS             complement(729..>1420)
                     /locus_tag="PK281_11945"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HOY50101.1"
                     /translation="DYPDQNGQLDDWIELYNADTQTVSIAGYYITDLISNPTKFQFPQ
                     ITAQTTMEPGSFKVLWADQNTIQGPLHLNFTLSATGGFVGLFAPDGETLIDGAQYPAM
                     SEGNSYGRYLDGADNWKLLFYATPGAPNSDSIPPVGILSGIDFESGISLFPNPANQQV
                     NVVKTGEALTGSINLYVMDLQGRKITAPVNNLIFGKRWTIDTSTLPSGSYILVMQSRD
                     GIRNARFSKTN"
BASE COUNT          444 a          301 c          265 g          410 t
ORIGIN      
        1 caggtacaat gtaaggagca gtacaaccat tattaccgta cgaacaaact cccgtttgcg
       61 tatattctgc taaagctcca tcgccaccaa gtaataaaga tcggtcgtat gaaacagttt
      121 gagtacaaga agatggacta ttaaaagtca tggataaatt ccgaatccct gaaccaacat
      181 tcgcaaaatt cccaataatc tcggttgaac aatggaaaat cacataaagc gtatccgcta
      241 aaccagcgaa tgaatttaaa gctggattga agttgattcc tgagatcagg atcactttcg
      301 agttagcagg caatactcca ccggtaggtt ctttcaataa tccacaatgc tcgatagtag
      361 cattcatggc agctacttta gcgcttgttg aagcattctg acaaacacca tcccagctat
      421 ttccagaagt agcccaagct acattgagat cggcagtatt aagggcattg ggaccgacaa
      481 gaaaacggac catttcgttt tgtccttcat cgttcccaca ggcatcaact aagatggact
      541 caatgcgaaa acatgaagag gttgtttgag cttgcgctaa gctgctcaag aacactaaaa
      601 tccacaaaca gaaaattctc agaggttgat tcctcatgtg cgttaaaacg gaacaataca
      661 aaggtaatgt aaatacgaaa accttgtctt attttattgt taatccgatt tcaactgtat
      721 gttccgaatt aattggtttt cgagaatcga gcatttcgga ttccatctct actttgcatc
      781 accaaaatat acgaaccgct tggtaatgtt gatgtatcta tcgtccaacg tttaccaaaa
      841 atcaagttat ttactggagc cgtgattttt cttccttgca aatccataac atacaggtta
      901 atcgaacccg ttaaggcttc tcctgtttta accacattta cttgctgatt tgcaggattt
      961 gggaataatg aaataccact ttcgaagtca attcctgata aaattccaac tggaggaatg
     1021 ctatccgagt taggcgctcc gggtgtagca taaaacaaca atttccaatt atcagcgcca
     1081 tccaaataac gaccatagga attgccttca ctcattgctg gatattgagc accatcaatt
     1141 agcgtttctc catcgggtgc aaataaacct acaaaaccac ctgttgctga taaagtaaag
     1201 ttgaggtgta aaggaccttg aatcgtgttt tgatcagccc ataacacttt gaaacttccc
     1261 ggttccatgg tagtttgagc tgttatttgt ggaaactgaa atttcgtagg attgcttatc
     1321 aaatcagtaa tgtagtaacc agcaatactt accgtttgtg tatcggcatt gtataactct
     1381 atccaatcat ctaattgtcc gttttgatct ggataatcgg
//
LOCUS       DAOOQW010000328         1347 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_133450_length_1347_cov_4.159443, whole genome shotgun
            sequence.
ACCESSION   DAOOQW010000328 DAOOQW010000000
VERSION     DAOOQW010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1347
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133450_length_1347_cov_4.159443"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            447..947
                     /locus_tag="PK281_11950"
     CDS             447..947
                     /locus_tag="PK281_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014085311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY50102.1"
                     /translation="MKKLLALGAFCLIAQMSFAQYALNKGQAQFNAGVGLSSWGVPVY
                     AGFDIGVHRDISVGGEMTFRSYYHGYDNNRYHHSIVGLAGNGNYHFNTVLNMPRNWDF
                     YAGLNLGFYFWNSDNDYRGDHTSGLQLGAQVGGRYYFTDRFGLNLEVGGAPSLSGGKF
                     GITLKL"
BASE COUNT          388 a          253 c          278 g          428 t
ORIGIN      
        1 aatgtagcct ggatgccagc taacctggta tacgctatgg aaaccattat gttgattctg
       61 tttggcgtgg cgtggctcgt aaagggaaaa tcaaaaccaa ccgaggtttt attgaaaagc
      121 atttagccaa cgccagttcc agcaaacgct actgtaaatt tatagatcct taacaaatag
      181 tgactcttaa gtacacgatc actattgggc aattaagata tctttttcat tccttacttt
      241 aagtcgaatt ccagcgcttt tcatcttatt gggtgaacat ttctattgag aagtaacgta
      301 gaattctgtg gcactttatc acttgtgtgg ttcattctcc tcactctgcc ccatcagttg
      361 gtctcaaaaa gtcgatttaa aatcggtatg atgatttcat aagcatgtag aaagtgatcc
      421 ttccatcaca atctaaaaaa cgaaatatga aaaagttact cgcactcggt gcattctgtt
      481 tgatcgcaca aatgagtttt gctcaatatg cattgaataa aggacaagct cagttcaatg
      541 ctggcgttgg attgtcatcc tggggagtgc ctgtatatgc aggctttgac atcggagttc
      601 atcgcgatat atccgtggga ggagaaatga ctttccgatc ctactaccat ggctatgata
      661 ataaccgata tcatcatagc attgtaggtt tggcaggtaa cggtaattat catttcaata
      721 ccgtattgaa tatgccaaga aattgggatt tctatgccgg cctaaatctt ggattctact
      781 tttggaattc agacaatgac tatcgcggtg atcacacttc agggctacaa ctcggagcac
      841 aggttggtgg gcgctattat ttcactgacc gatttggttt aaacttagaa gtaggtggcg
      901 caccttccct ttcgggtggc aagtttggaa ttacgcttaa gctttaatcg aactaagtgt
      961 aaagtagaat gcatgagaga atttatttct tttgtgcatt cctttcattt ttcagccgaa
     1021 gaatttaccg gtttaccttt gcgggctcat tcttaatcct aacaacaata ttctccatta
     1081 atcgctttag attgttcggt aaactaaatt gtatgggaga agacgcagag gcggttgtct
     1141 tttcatagcc agccagaatc cgtggatcaa tcaatccatt catggtatat aaagatgctt
     1201 gtctggcgaa atcatttctg gttttcattt gaatatcatt ttacaataca aagaaacggt
     1261 gattagatct tgtaggtgct tgtaagtaat gaaatcgtaa aactagtcga tgcaatgcgt
     1321 tcaaaatgaa atcttaagca aacggtt
//
LOCUS       DAOOQW010000329         1140 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_176455_length_1140_cov_2.623963, whole genome shotgun
            sequence.
ACCESSION   DAOOQW010000329 DAOOQW010000000
VERSION     DAOOQW010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_176455_length_1140_cov_2.623963"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1047
                     /locus_tag="PK281_11955"
     CDS             <1..1047
                     /locus_tag="PK281_11955"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOY50103.1"
                     /translation="VSGTSTGAIISGLILAGKSAIEIEELYKSLVTRVFKKRSLTSSR
                     TINPPEFDKVNYRDELRQLFTDETLEYLSKQSGIDCLFTSKDMAAGEEVFFTAIQNNG
                     SKGTYKSSLMRGVLEATMSAPTYFLPFERFIDGGTTTFNNPVLAAAMEAFYYDGKGKY
                     TPENTTIFSFGTGTCLRFVDPNKTSNPDGIDAFFWLNYVMDESSKDASEMQVNMLRSG
                     LIPHLDFRRYQLSLDMNTLNKMPDKDISKHGIKNKEWLHQLTDKELAKIDMADVTKFG
                     LMEAIGEAAADFICPPAESQIPLANRSGNWFQKDLLAPNSKSRGALVSARGNIAQIQS
                     HLSNPAWIDSVKTE"
BASE COUNT          374 a          218 c          216 g          332 t
ORIGIN      
        1 gtttctggca cgtcaacagg agctattatt tcgggattaa ttctcgcagg aaaatcggct
       61 atcgaaattg aagaactata taaatcatta gtcactcggg tatttaagaa acgatcgtta
      121 acatcaagta ggacaataaa tcctcccgaa tttgataaag taaattatcg ggatgaatta
      181 cgacaacttt tcaccgacga aaccttagaa tatctttcta agcaatcggg tatcgactgt
      241 ttattcacat ccaaagatat ggcagctggt gaagaagtat tctttacggc tatccaaaat
      301 aatggctcaa aaggaactta caaaagttca ttaatgcgag gtgttttaga ggctactatg
      361 tcggctccaa cgtattttct accttttgaa cggttcattg atggaggaac tacaaccttt
      421 aacaatccgg ttttagcggc tgcaatggaa gcattctatt acgatggaaa aggaaaatac
      481 acacccgaaa acacgaccat tttcagcttt ggaacaggaa cctgtcttcg ctttgttgat
      541 ccaaacaaaa catccaatcc tgatggtatt gatgcatttt tctggctcaa ttatgtcatg
      601 gatgaatcga gtaaagatgc tagtgaaatg caagtaaaca tgcttcgaag tggcttgatt
      661 cctcatctcg attttagaag atatcagcta tcgctcgata tgaatacctt gaacaaaatg
      721 cctgataagg atatcagtaa acatggaatt aaaaacaaag aatggttgca tcaattaacg
      781 gataaagaac tcgccaaaat cgatatggct gatgtaacta aattcggact catggaagct
      841 ataggtgaag cagcagccga ttttatttgt ccacctgctg aatcacaaat tcctcttgcc
      901 aatcgcagcg gaaattggtt tcaaaaagat ttattagcac ctaattccaa atcgcgtggg
      961 gctcttgttt cggctcgcgg taatattgca caaatacaaa gtcatttatc gaatcctgct
     1021 tggattgatt ccgtcaaaac agaataaaac tcttcattct tattcatccg attcccaaga
     1081 tttggtattc gaaaaatcac ggaataataa ttttccaaaa tcttcgggat tgctagtata
//
LOCUS       DAOOQW010000330         1064 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_198607_length_1064_cov_4.407334, whole genome shotgun
            sequence.
ACCESSION   DAOOQW010000330 DAOOQW010000000
VERSION     DAOOQW010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_198607_length_1064_cov_4.407334"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..799)
                     /locus_tag="PK281_11960"
     CDS             complement(<1..799)
                     /locus_tag="PK281_11960"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:NF016084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HOY50104.1"
                     /translation="MSDGAEHLVRVGITQGDFNGIGYEIIMKTFMDHGMLELCTPIVY
                     GVHKAATQHRKFLEKNEFNFFGIHDATKAAKHHCNFINLTDIDVGLDFGKATPAAGAW
                     AVKSLQRAMEDLQAGLIDVLVTAPFNKSTVQGPDFNFPGHTEFLAERTNSKDFLMLLV
                     SENLRVGTVTGHVPIQQVAGLLSTEKILSKLKVMNQSLLQDFGIRKPRIAVLGLNPHA
                     GDSGLIGNEENQIIIPAINQANNEGILAFGPYSADGFFGSGTWKKFDA"
     gene            complement(796..>1064)
                     /locus_tag="PK281_11965"
     CDS             complement(796..>1064)
                     /locus_tag="PK281_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase M17"
                     /protein_id="HOY50105.1"
                     /translation="ERLVEFPLWDDYSELLKSDIADIKNIGGITAGANTAGKFLERFT
                     DYPWVHLDIAGPAFVESPDSYRGKGGTGSGVRLLFDFIANKAKA"
BASE COUNT          299 a          248 c          189 g          328 t
ORIGIN      
        1 ccgcatcaaa ctttttccac gtcccacttc caaaaaatcc atcagccgaa taaggtccaa
       61 aagctagaat tccttcgtta ttcgcttgat tgatagctgg gatgatgatt tgattttctt
      121 cgtttcctat caaaccacta tcgccagcat gtggatttaa tccaagcaca gcaattctag
      181 gtttacgaat tccaaaatct tgcaacaagg attgattcat caccttaagc ttactcagaa
      241 tcttttcggt acttaataat cctgcaactt gctgaatggg tacatgtccg gttacagttc
      301 caacacgaag attttcactt accaaaagca tcaaaaaatc tttgctgtta gttcgttcgg
      361 ctagaaattc tgtatgacct gggaaattaa aatctggacc ttgaaccgtg cttttgttaa
      421 atggtgcagt aactaaaacg tcaatcaaac ctgcttgtaa atcttccata gcgcgctgca
      481 atgactttac agcccatgca cctgctgctg gagtagcttt tccaaaatca agtcctacgt
      541 cgatatcagt aaggttgata aaattgcaat gatgtttagc cgctttagtt gcatcgtgaa
      601 ttccgaaaaa attgaattcg tttttctcta agaatttccg gtgttgtgtg gcagccttgt
      661 gaacaccata aacaatgggt gtacaaagtt cgagcatacc atgatccatg aaggttttca
      721 tgataatctc atacccaatt ccattaaaat ctccttgcgt aattcctacc cgtacaaggt
      781 gttcagctcc gtcgctcatg cttttgcttt gttcgctatg aaatcaaata acaatcgaac
      841 tcccgatcct gttccacctt ttccacgata tgaatcgggc gactctacga atgctggtcc
      901 ggcgatgtcc aaatgcaccc aaggataatc ggtaaatcgc tcaaggaatt tccctgcagt
      961 attcgctccg gctgttattc ctccaatatt ttttatgtcg gcaatatcgc ttttgagtaa
     1021 ttcggaataa tcatcccaaa gcggaaattc aaccaatcgt tcgt
//
LOCUS       DAOOQW010000331         1009 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_2561
            NODE_217771_length_1009_cov_4.300839, whole genome shotgun
            sequence.
ACCESSION   DAOOQW010000331 DAOOQW010000000
VERSION     DAOOQW010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864636
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,395
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,364
            CDSs (with protein)               :: 2,364
            Genes (RNA)                       :: 20
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_217771_length_1009_cov_4.300839"
                     /isolate="AMR_MDS_2561"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="PK281_11970"
     CDS             complement(<1..344)
                     /locus_tag="PK281_11970"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HOY50106.1"
                     /translation="MSLIGNFLSPGARIFKLLAYNGTRDELRGLGLVDWLSKRVIPDD
                     PQVVVKEVLVEGMDSAWFEPKKQMSDKVMLYFHGGGYCVCSWQTHRSLISRLSRAAGM
                     KTLAVNYRLAPE"
     gene            complement(366..>1009)
                     /locus_tag="PK281_11975"
     CDS             complement(366..>1009)
                     /locus_tag="PK281_11975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY50107.1"
                     /translation="LAKYLFIAAKEDNASLNTHQEKAFFSHIFERIQLQRDLHFHTQT
                     SIDTLDYSGDGLNTGSKLVLAAAGKKRRELGTQIPTLSLPQNFSQIHIALPGVLTISG
                     AKFQSYSDAEIQMHELIASIPDSLEQTFPLIVVCDDAEFTAQTFNNWVWVTFTRSNPS
                     HDIYGVDAFQKNKHWGCNGPLIIDARIKPHHAPVLELDSNVESRVDSILSQIN"
BASE COUNT          316 a          204 c          195 g          294 t
ORIGIN      
        1 ttttcgggag ctaatcggta gttcacggct aatgttttca tacctgctgc cctactcaat
       61 cgactaatta aactacgatg cgtttgccaa gaacatacac aatatccacc tccgtgaaaa
      121 taaagcatga ctttatctga catttgtttc ttaggctcaa accatgccga atccattcct
      181 tccacaagta cttctttaac aacaacttga ggatcatcgg gaatgacacg tttagaaagc
      241 caatcgacca aacctaatcc acgtaattca tcgcgggtac cgttataggc aagcaattta
      301 aaaatgcgag cacccggact taagaaattt ccaatgagcg acatggttta aaaataagaa
      361 atcgattaat ttatttgtga aaggattgaa tctactctac tttcgacatt cgaatcgagt
      421 tccaacactg gagcgtgatg aggtttgatt cgagcgtcga taataagtgg accattgcag
      481 ccccaatgtt tatttttctg aaacgcatcc acaccatata tatcatgtga aggattacta
      541 cgagtaaacg taacccaaac ccaattgttg aacgtttggg cagtaaattc tgcatcatcg
      601 caaacaacta ttagcggaaa agtctgttcc aaactatctg gaatactagc tatcagttcg
      661 tgcatttgaa tttcagcatc cgaatatgat tgaaatttag caccgctaat tgtaagaact
      721 ccaggtaatg caatatgaat ttgagagaaa ttttggggta gtgaaagggt aggaatttga
      781 gtgccaagtt cccttcgttt ttttccagcc gcggctaaaa ccaatttcga tccggtattc
      841 aacccgtcgc cactataatc aagtgtatcg atactggttt gagtatgaaa atgcaaatca
      901 cgttgaagct gtatgcgttc gaaaatgtgc gaaaaaaatg ctttttcctg atgggtgtta
      961 agagatgcat tatcttcttt agctgcaata aataggtatt tggcaagac
//
